LOCUS       BBa_K1820020            1125 bp    DNA     linear       13-Apr-2026
DEFINITION  P335-Φ31 inducible middle promoter + RBS+ sfGFP + term
ACCESSION   BBa_K1820020
VERSION     BBa_K1820020.1  
FEATURES             Location/Qualifiers
     misc_feature    144..144
                     /label=Transcription Start Site A
     misc_feature    1..6
                     /label=BamHI
     misc_feature    132..137
                     /label=-10 Region A
     misc_feature    172..177
                     /label=-10 Region B
     misc_feature    185..185
                     /label=Transcription Start Site B
     promoter        1..245
                     /label=BBa_K1820000
     misc_feature    258..261
                     /label=conserved
     RBS             254..265
                     /label=BBa_B0034
     misc_feature    1..265
                     /label=BBa_K1820001
     misc_feature    272..274
                     /label=start codon
     CDS             275..985
                     /label=sfGFP
     misc_feature    985..988
                     /label=stop codon
     misc_feature    272..988
                     /label=BBa_K1820004
     stem_loop       1008..1051
                     /label=stem_loop
     misc_feature    997..1076
                     /label=BBa_B0010
     terminator      997..1076
                     /label=BBa_B0010
     stem_loop       1092..1111
                     /label=T7 TE
     misc_feature    1118..1118
                     /label=stop
     polyA_site      1112..1125
                     /label=polya
     misc_feature    1085..1125
                     /label=BBa_B0012
     terminator      1085..1125
                     /label=BBa_B0012
     terminator      997..1125
                     /label=BBa_B0015
     misc_feature    272..1125
                     /label=BBa_K1820006
ORIGIN
        1 ggatccgtgt cacataactg agcgccaata ctttagaaag agaagagcaa tacttgagaa
       61 gtatgatgag atatgtgacg gcttctggta atttgtcacc ttttgggcga aaactgacaa
      121 gataaatgtt gtattatagt atcatcaaat aaaacaaata aagccagcgg atatattctg
      181 ttggcttttt gtgtggagaa agtgaggtga cctcccatag cattacgtgc tgaccgtact
      241 ggtgctacta gagaaagagg agaaatacta gatgtcaaaa ggagaagagc tgttcacagg
      301 tgttgtgccg attctcgttg agcttgacgg agatgtaaac ggacacaaat tctctgttcg
      361 cggtgaaggt gaaggagatg caacaaacgg caagctgaca ttgaagttta tttgcacaac
      421 tggaaagctg ccggttcctt ggccgacact tgtaacgacg ctgacttacg gcgttcaatg
      481 cttctctcgt tatccagacc acatgaaacg ccatgatttc ttcaaatctg caatgcctga
      541 aggctacgtt caagagcgta cgatcagctt caaagatgac ggaacgtaca aaacaagagc
      601 agaagtgaag tttgaaggtg acacacttgt gaaccgcatt gaattgaaag gcattgattt
      661 caaagaagat ggaaacatcc ttggacacaa acttgaatac aacttcaaca gccacaacgt
      721 atacatcact gctgacaaac aaaaaaacgg catcaaagca aacttcaaaa tccgtcataa
      781 cgtagaggac ggttctgttc agcttgctga tcattatcag caaaatacac cgatcggtga
      841 cggcccggtt cttcttcctg ataaccatta tttatcaact caaagcgtat tatcaaaaga
      901 cccaaatgaa aagcgtgacc acatggtgct gcttgaattt gtgacagctg ctggtatcac
      961 tcacggcatg gatgagcttt ataagtaata ctagagccag gcatcaaata aaacgaaagg
     1021 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact
     1081 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttata
//