BBa_K176092
type: ComponentDefinition
persistent identity: BBa K176092
display id: BBa_K176092
version: 1
was generated by: iGEM to SBOL conversion
description: Cell density controller: pCon max->luxI-LVA+pCon 0.70->luxR+pLux/Tet->lacZalpha-ccdB(weak RBS)
created: 2009-08-09T11:00:00Z
modified: 2015-05-08T01:11:02Z
mutable provenance: K176081 K176063
owned by: james
owned by: myers
mutable description: Cell Death = (Cell Density * pCon max) + Input AHL
discontinued: false
dominant: false
experience: experience/None
group u list: _282_
m user id: 0
owner id: 3908
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Input: AHL Output1: AHL Output2: Cell Density Design: pCon max(J23119) generates LuxI-LVA, LuxI-LVA generates Output1 AHL, the concentration of AHL in the medium depends on both LuxI-LVA level per cell and cell density. pCon 0.70(J23101) generates LuxR. pLux/Tet(K176000) generates ccdB to kill the cell, activated by LuxR and (Output1 AHL generated by LuxI-LVA + Input AHL), repressed by TetR and no aTc.
star: false
creator: Danqian Liu, Hao Jiang
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K176063
component: BBa_K176081
sequence annotation: BBa_K176081
sequence annotation: BBa_K176063
sequence: https://synbiohub.org/public/igem/BBa_K176092_sequence/1

BBa_K176063
type: Component
persistent identity: component2306934
display id: component2306934
version: 1
top level: BBa_K176092
definition: BBa_K176063
access: public

BBa_K176081
type: Component
persistent identity: component2306912
display id: component2306912
version: 1
top level: BBa_K176092
definition: BBa_K176081
access: public

BBa_K176081
type: SequenceAnnotation
persistent identity: annotation2306912
display id: annotation2306912
version: 1
top level: BBa_K176092
location: https://synbiohub.org/public/igem/BBa_K176092/annotation2306912/range2306912/1
component: BBa_K176081

https://synbiohub.org/public/igem/BBa_K176092/annotation2306912/range2306912/1
type: Range
persistent identity: range2306912
display id: range2306912
version: 1
top level: BBa_K176092
direction: direction/reverse
start: 1
end: 1908
orientation: inline

BBa_K176063
type: SequenceAnnotation
persistent identity: annotation2306934
display id: annotation2306934
version: 1
top level: BBa_K176092
location: https://synbiohub.org/public/igem/BBa_K176092/annotation2306934/range2306934/1
component: BBa_K176063

https://synbiohub.org/public/igem/BBa_K176092/annotation2306934/range2306934/1
type: Range
persistent identity: range2306934
display id: range2306934
version: 1
top level: BBa_K176092
direction: direction/reverse
start: 1917
end: 2553
orientation: inline

BBa_K176063
type: ComponentDefinition
persistent identity: BBa K176063
display id: BBa_K176063
version: 1
was generated by: iGEM to SBOL conversion
description: PoPS->RBS(weak)+lacZalpha-ccdB+T
created: 2009-08-06T11:00:00Z
modified: 2015-05-08T01:11:02Z
mutable provenance: K176061 B0015
owned by: james
owned by: myers
mutable description: Generate lacZalpha-ccdB (weak)
discontinued: false
dominant: false
experience: experience/None
group u list: _282_
m user id: 0
owner id: 3908
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: weak RBS + lacZalpha-ccdB coding + strong terminator
star: false
creator: Zongxiao He, Hao Jiang
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0012
component: BBa_B0010
component: BBa_K176003
component: BBa_B0031
sequence annotation: BBa_B0031
sequence annotation: BBa_K176003
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K176063_sequence/1

BBa_B0012
type: Component
persistent identity: component2015454
display id: component2015454
version: 1
top level: BBa_K176063
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component2015452
display id: component2015452
version: 1
top level: BBa_K176063
definition: BBa_B0010
access: public

BBa_K176003
type: Component
persistent identity: component2015451
display id: component2015451
version: 1
top level: BBa_K176063
definition: lacZa-ccdB
access: public

BBa_B0031
type: Component
persistent identity: component2015438
display id: component2015438
version: 1
top level: BBa_K176063
definition: BBa_B0031
access: public

BBa_B0031
type: SequenceAnnotation
persistent identity: annotation2015438
display id: annotation2015438
version: 1
top level: BBa_K176063
location: https://synbiohub.org/public/igem/BBa_K176063/annotation2015438/range2015438/1
component: BBa_B0031

https://synbiohub.org/public/igem/BBa_K176063/annotation2015438/range2015438/1
type: Range
persistent identity: range2015438
display id: range2015438
version: 1
top level: BBa_K176063
direction: direction/reverse
start: 1
end: 14
orientation: inline

BBa_K176003
type: SequenceAnnotation
persistent identity: annotation2015451
display id: annotation2015451
version: 1
top level: BBa_K176063
location: https://synbiohub.org/public/igem/BBa_K176063/annotation2015451/range2015451/1
component: BBa_K176003

https://synbiohub.org/public/igem/BBa_K176063/annotation2015451/range2015451/1
type: Range
persistent identity: range2015451
display id: range2015451
version: 1
top level: BBa_K176063
direction: direction/reverse
start: 21
end: 500
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2015452
display id: annotation2015452
version: 1
top level: BBa_K176063
location: https://synbiohub.org/public/igem/BBa_K176063/annotation2015452/range2015452/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K176063/annotation2015452/range2015452/1
type: Range
persistent identity: range2015452
display id: range2015452
version: 1
top level: BBa_K176063
direction: direction/forward
start: 509
end: 588
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2015454
display id: annotation2015454
version: 1
top level: BBa_K176063
location: https://synbiohub.org/public/igem/BBa_K176063/annotation2015454/range2015454/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K176063/annotation2015454/range2015454/1
type: Range
persistent identity: range2015454
display id: range2015454
version: 1
top level: BBa_K176063
direction: direction/forward
start: 597
end: 637
orientation: inline

BBa_K176081
type: ComponentDefinition
persistent identity: BBa K176081
display id: BBa_K176081
version: 1
was generated by: iGEM to SBOL conversion
description: cell density->PoPS: pCon max->luxI-LVA+pCon 0.70->luxR+pLux/Tet->PoPS
created: 2009-08-09T11:00:00Z
modified: 2015-05-08T01:11:02Z
mutable provenance: K176018 K176028
owned by: james
owned by: myers
mutable description: Output PoPS = Cell Density + Input AHL
discontinued: false
dominant: false
experience: experience/None
group u list: _282_
m user id: 0
owner id: 3908
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: Input: AHL Output1: AHL Output2: PoPS Design: pCon max(J23119) generates LuxI-LVA, LuxI-LVA generates Output1 AHL, the concentration of AHL in the medium depends on both LuxI-LVA level per cell and cell density. pCon 0.70(J23101) generates LuxR. pLux/Tet(K176000) generates Output2 PoPS, activated by LuxR and (Output1 AHL generated by LuxI-LVA + Input AHL), repressed by TetR and no aTc
star: false
creator: Danqian Liu, Hao Jiang
type: DnaRegion
role: SO:0000804
role: partType/Device
component: BBa_K176028
component: BBa_K176018
sequence annotation: BBa_K176018
sequence annotation: BBa_K176028
sequence: https://synbiohub.org/public/igem/BBa_K176081_sequence/1

BBa_K176028
type: Component
persistent identity: component2293081
display id: component2293081
version: 1
top level: BBa_K176081
definition: BBa_K176028
access: public

BBa_K176018
type: Component
persistent identity: component2293056
display id: component2293056
version: 1
top level: BBa_K176081
definition: BBa_K176018
access: public

BBa_K176018
type: SequenceAnnotation
persistent identity: annotation2293056
display id: annotation2293056
version: 1
top level: BBa_K176081
location: https://synbiohub.org/public/igem/BBa_K176081/annotation2293056/range2293056/1
component: BBa_K176018

https://synbiohub.org/public/igem/BBa_K176081/annotation2293056/range2293056/1
type: Range
persistent identity: range2293056
display id: range2293056
version: 1
top level: BBa_K176081
direction: direction/reverse
start: 1
end: 841
orientation: inline

BBa_K176028
type: SequenceAnnotation
persistent identity: annotation2293081
display id: annotation2293081
version: 1
top level: BBa_K176081
location: https://synbiohub.org/public/igem/BBa_K176081/annotation2293081/range2293081/1
component: BBa_K176028

https://synbiohub.org/public/igem/BBa_K176081/annotation2293081/range2293081/1
type: Range
persistent identity: range2293081
display id: range2293081
version: 1
top level: BBa_K176081
direction: direction/reverse
start: 850
end: 1908
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

BBa_B0031
type: ComponentDefinition
persistent identity: BBa B0031
display id: BBa_B0031
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.2 (weak) -- derivative of BBa_0030
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Medium RBS based on Ron Weiss thesis. Strength considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-1" in figure 4-14 of thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Cho
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0031_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23316
display id: annotation23316
version: 1
top level: BBa_B0031
location: https://synbiohub.org/public/igem/BBa_B0031/annotation23316/range23316/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0031/annotation23316/range23316/1
type: Range
persistent identity: range23316
display id: range23316
version: 1
top level: BBa_B0031
direction: direction/forward
start: 7
end: 10
orientation: inline

BBa_K176018
type: ComponentDefinition
persistent identity: BBa K176018
display id: BBa_K176018
version: 1
was generated by: iGEM to SBOL conversion
description: pCon max(J23119)->RBS+luxI-LVA+T
created: 2009-06-30T11:00:00Z
modified: 2015-05-08T01:11:01Z
mutable provenance: J23119 K082014
owned by: james
owned by: myers
mutable description: pCon(J23119)+luxI(K082014)
discontinued: false
dominant: false
experience: experience/Works
group u list: _282_
m user id: 0
owner id: 3908
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: pCon(J23119) generate LuxI
star: false
creator: Danqian Liu, Chao Li, Hao Jiang
type: DnaRegion
role: SO:0000804
role: partType/Signalling
component: BBa_J23119
component: BBa_K082014
sequence annotation: BBa_J23119
sequence annotation: BBa_K082014
sequence: https://synbiohub.org/public/igem/BBa_K176018_sequence/1

BBa_J23119
type: Component
persistent identity: component2255168
display id: component2255168
version: 1
top level: BBa_K176018
definition: BBa_J23119
access: public

BBa_K082014
type: Component
persistent identity: component2255183
display id: component2255183
version: 1
top level: BBa_K176018
definition: BBa_K082014
access: public

BBa_J23119
type: SequenceAnnotation
persistent identity: annotation2255168
display id: annotation2255168
version: 1
top level: BBa_K176018
location: https://synbiohub.org/public/igem/BBa_K176018/annotation2255168/range2255168/1
component: BBa_J23119

https://synbiohub.org/public/igem/BBa_K176018/annotation2255168/range2255168/1
type: Range
persistent identity: range2255168
display id: range2255168
version: 1
top level: BBa_K176018
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_K082014
type: SequenceAnnotation
persistent identity: annotation2255183
display id: annotation2255183
version: 1
top level: BBa_K176018
location: https://synbiohub.org/public/igem/BBa_K176018/annotation2255183/range2255183/1
component: BBa_K082014

https://synbiohub.org/public/igem/BBa_K176018/annotation2255183/range2255183/1
type: Range
persistent identity: range2255183
display id: range2255183
version: 1
top level: BBa_K176018
direction: direction/reverse
start: 44
end: 841
orientation: inline

lacZa-ccdB
type: ComponentDefinition
persistent identity: BBa K176003
display id: BBa_K176003
version: 1
was generated by: iGEM to SBOL conversion
description: lacZalpha-ccdB coding sequence
created: 2009-07-02T11:00:00Z
modified: 2015-07-09T11:37:13Z
mutable provenance: PCR from the plasmid pluxCcdB3(a gift from Prof. Lingchong You). Forward Primer: GTTTCT TCTAG ATG accatgattacgg attcactggccgtcgttttac Reverse Primer: GTTTCTTC CTGCAG CGGCCGC T ACTAGT A TTATTA tattccccagaacatcaggtta Digest with XbaI and SpeI, ligate into pSB1A3.
owned by: james
owned by: myers
mutable description: A fusion protein of lacZalpha(I732006) and ccdB(K145151). It should have the function of ccdB to cause E. coli cells to die, but have less toxicity because of the lacZalpha part. It should also have the function of lacZalpha as a reporter for measurement. This part is similar to J32026 but the multiple cloning sites are eliminated. It is constructed by PCR from the plasmid pluxCcdB3(a gift from Prof. Lingchong You).
discontinued: true
dominant: false
experience: experience/Works
group u list: _282_
m user id: 4206
owner id: 3908
owning group id: 9
sample status: It's complicated
status: status/Deleted
bookmark: true
mutable notes: The part is similar to BBa_J32026 and the original coding sequence in pluxCcdB3, but the multiple cloning sites are eliminated in order to avoid site-specific mutagenesis. The multiple cloning sites are from lacZalpha in pZErO-2. After eliminate the MCS, the lacZalpha part is identical to the N-terminal of the wild type gene(BBa_I732006 & BBa_I732005), and fused with the ccdB part (BBa_K145151) with a 3aa linker.
star: false
creator: Zongxiao He, Hao Jiang
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: lacZalpha-ccdB
sequence annotation: Start
sequence annotation: a part of lacZalpha (I732006)
sequence annotation: 177bp and a in J32026
sequence annotation: M13 -20 primer
sequence annotation: M13 -40 primer
sequence annotation: M13 -47 primer (K176059)
sequence annotation: g and 69bp in I732006
sequence annotation: ccdB (K145151)
sequence annotation: at in K145151
sequence annotation: VRccdB primer (K176058)
sequence annotation: c in K145151
sequence annotation: Stop
sequence: https://synbiohub.org/public/igem/BBa_K176003_sequence/1

lacZalpha-ccdB
type: SequenceAnnotation
persistent identity: annotation2020665
display id: annotation2020665
version: 1
top level: lacZa-ccdB
location: https://synbiohub.org/public/igem/BBa_K176003/annotation2020665/range2020665/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K176003/annotation2020665/range2020665/1
type: Range
persistent identity: range2020665
display id: range2020665
version: 1
top level: lacZa-ccdB
direction: direction/forward
start: 1
end: 474
orientation: inline

Start
type: SequenceAnnotation
persistent identity: annotation2008001
display id: annotation2008001
version: 1
top level: lacZa-ccdB
location: https://synbiohub.org/public/igem/BBa_K176003/annotation2008001/range2008001/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_K176003/annotation2008001/range2008001/1
type: Range
persistent identity: range2008001
display id: range2008001
version: 1
top level: lacZa-ccdB
direction: direction/forward
start: 1
end: 3
orientation: inline

a part of lacZalpha (I732006)
type: SequenceAnnotation
persistent identity: annotation2008000
display id: annotation2008000
version: 1
top level: lacZa-ccdB
location: https://synbiohub.org/public/igem/BBa_K176003/annotation2008000/range2008000/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K176003/annotation2008000/range2008000/1
type: Range
persistent identity: range2008000
display id: range2008000
version: 1
top level: lacZa-ccdB
direction: direction/forward
start: 1
end: 165
orientation: inline

177bp and a in J32026
type: SequenceAnnotation
persistent identity: annotation2008002
display id: annotation2008002
version: 1
top level: lacZa-ccdB
location: https://synbiohub.org/public/igem/BBa_K176003/annotation2008002/range2008002/1
role: feature/mutation
role: SO:0001059

https://synbiohub.org/public/igem/BBa_K176003/annotation2008002/range2008002/1
type: Range
persistent identity: range2008002
display id: range2008002
version: 1
top level: lacZa-ccdB
direction: direction/forward
start: 16
end: 16
orientation: inline

M13 -20 primer
type: SequenceAnnotation
persistent identity: annotation2008003
display id: annotation2008003
version: 1
top level: lacZa-ccdB
location: https://synbiohub.org/public/igem/BBa_K176003/annotation2008003/range2008003/1
role: feature/primer binding
role: SO:0005850

https://synbiohub.org/public/igem/BBa_K176003/annotation2008003/range2008003/1
type: Range
persistent identity: range2008003
display id: range2008003
version: 1
top level: lacZa-ccdB
direction: direction/reverse
start: 21
end: 37
orientation: inline

M13 -40 primer
type: SequenceAnnotation
persistent identity: annotation2008005
display id: annotation2008005
version: 1
top level: lacZa-ccdB
location: https://synbiohub.org/public/igem/BBa_K176003/annotation2008005/range2008005/1
role: feature/primer binding
role: SO:0005850

https://synbiohub.org/public/igem/BBa_K176003/annotation2008005/range2008005/1
type: Range
persistent identity: range2008005
display id: range2008005
version: 1
top level: lacZa-ccdB
direction: direction/reverse
start: 41
end: 57
orientation: inline

M13 -47 primer (K176059)
type: SequenceAnnotation
persistent identity: annotation2008004
display id: annotation2008004
version: 1
top level: lacZa-ccdB
location: https://synbiohub.org/public/igem/BBa_K176003/annotation2008004/range2008004/1
role: feature/primer binding
role: SO:0005850

https://synbiohub.org/public/igem/BBa_K176003/annotation2008004/range2008004/1
type: Range
persistent identity: range2008004
display id: range2008004
version: 1
top level: lacZa-ccdB
direction: direction/reverse
start: 41
end: 64
orientation: inline

g and 69bp in I732006
type: SequenceAnnotation
persistent identity: annotation2008006
display id: annotation2008006
version: 1
top level: lacZa-ccdB
location: https://synbiohub.org/public/igem/BBa_K176003/annotation2008006/range2008006/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_K176003/annotation2008006/range2008006/1
type: Range
persistent identity: range2008006
display id: range2008006
version: 1
top level: lacZa-ccdB
direction: direction/forward
start: 165
end: 165
orientation: inline

ccdB (K145151)
type: SequenceAnnotation
persistent identity: annotation2008008
display id: annotation2008008
version: 1
top level: lacZa-ccdB
location: https://synbiohub.org/public/igem/BBa_K176003/annotation2008008/range2008008/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_K176003/annotation2008008/range2008008/1
type: Range
persistent identity: range2008008
display id: range2008008
version: 1
top level: lacZa-ccdB
direction: direction/forward
start: 172
end: 477
orientation: inline

at in K145151
type: SequenceAnnotation
persistent identity: annotation2008007
display id: annotation2008007
version: 1
top level: lacZa-ccdB
location: https://synbiohub.org/public/igem/BBa_K176003/annotation2008007/range2008007/1
role: feature/mutation
role: SO:0001059

https://synbiohub.org/public/igem/BBa_K176003/annotation2008007/range2008007/1
type: Range
persistent identity: range2008007
display id: range2008007
version: 1
top level: lacZa-ccdB
direction: direction/forward
start: 172
end: 173
orientation: inline

VRccdB primer (K176058)
type: SequenceAnnotation
persistent identity: annotation2011971
display id: annotation2011971
version: 1
top level: lacZa-ccdB
location: https://synbiohub.org/public/igem/BBa_K176003/annotation2011971/range2011971/1
role: feature/primer binding
role: SO:0005850

https://synbiohub.org/public/igem/BBa_K176003/annotation2011971/range2011971/1
type: Range
persistent identity: range2011971
display id: range2011971
version: 1
top level: lacZa-ccdB
direction: direction/reverse
start: 188
end: 215
orientation: inline

c in K145151
type: SequenceAnnotation
persistent identity: annotation2008009
display id: annotation2008009
version: 1
top level: lacZa-ccdB
location: https://synbiohub.org/public/igem/BBa_K176003/annotation2008009/range2008009/1
role: SO:1000008
role: feature/s mutation

https://synbiohub.org/public/igem/BBa_K176003/annotation2008009/range2008009/1
type: Range
persistent identity: range2008009
display id: range2008009
version: 1
top level: lacZa-ccdB
direction: direction/forward
start: 255
end: 255
orientation: inline

Stop
type: SequenceAnnotation
persistent identity: annotation2008010
display id: annotation2008010
version: 1
top level: lacZa-ccdB
location: https://synbiohub.org/public/igem/BBa_K176003/annotation2008010/range2008010/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K176003/annotation2008010/range2008010/1
type: Range
persistent identity: range2008010
display id: range2008010
version: 1
top level: lacZa-ccdB
direction: direction/forward
start: 475
end: 480
orientation: inline

BBa_K176028
type: ComponentDefinition
persistent identity: BBa K176028
display id: BBa_K176028
version: 1
was generated by: iGEM to SBOL conversion
description: TetR repressible AHL->PoPS Receiver (Very High LuxR): pCon 0.70->luxR+pLux/Tet->PoPS
created: 2009-07-19T11:00:00Z
modified: 2015-05-08T01:11:02Z
mutable provenance: K176025 K176000
owned by: james
owned by: myers
mutable description: AHL -> PoPS Receiver TetR repressible Similar to F2620, but use pLux/Tet hybrid promoter.
discontinued: false
dominant: false
experience: experience/Works
group u list: _282_
m user id: 0
owner id: 3908
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: Input: AHL Output: PoPS Design: pCon 0.70(J23101) generate LuxR pLux/Tet(K176000) generate PoPS output, activated by LuxR and AHL, repressed by TetR and no aTc
star: false
creator: Danqian Liu, Chao Li, Hao Jiang
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_K176000
component: BBa_K176025
sequence annotation: BBa_K176025
sequence annotation: BBa_K176000
sequence: https://synbiohub.org/public/igem/BBa_K176028_sequence/1

BBa_K176000
type: Component
persistent identity: component2262163
display id: component2262163
version: 1
top level: BBa_K176028
definition: pLux/Tet
access: public

BBa_K176025
type: Component
persistent identity: component2262156
display id: component2262156
version: 1
top level: BBa_K176028
definition: LuxR
access: public

BBa_K176025
type: SequenceAnnotation
persistent identity: annotation2262156
display id: annotation2262156
version: 1
top level: BBa_K176028
location: https://synbiohub.org/public/igem/BBa_K176028/annotation2262156/range2262156/1
component: BBa_K176025

https://synbiohub.org/public/igem/BBa_K176028/annotation2262156/range2262156/1
type: Range
persistent identity: range2262156
display id: range2262156
version: 1
top level: BBa_K176028
direction: direction/reverse
start: 1
end: 979
orientation: inline

BBa_K176000
type: SequenceAnnotation
persistent identity: annotation2262163
display id: annotation2262163
version: 1
top level: BBa_K176028
location: https://synbiohub.org/public/igem/BBa_K176028/annotation2262163/range2262163/1
component: BBa_K176000

https://synbiohub.org/public/igem/BBa_K176028/annotation2262163/range2262163/1
type: Range
persistent identity: range2262163
display id: range2262163
version: 1
top level: BBa_K176028
direction: direction/reverse
start: 988
end: 1059
orientation: inline

BBa_J23119
type: ComponentDefinition
persistent identity: BBa J23119
display id: BBa_J23119
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-23T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: Overlap extension of synthetic oligonucleotides
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Later
discontinued: false
dominant: true
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence: https://synbiohub.org/public/igem/BBa_J23119_sequence/1

BBa_K082014
type: ComponentDefinition
persistent identity: BBa K082014
display id: BBa_K082014
version: 1
was generated by: iGEM to SBOL conversion
description: C0261-B0015
created: 2008-10-28T12:00:00Z
modified: 2015-05-08T01:08:35Z
mutable provenance: none
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: none
discontinued: false
dominant: false
experience: experience/Works
group u list: _228_
m user id: 0
owner id: 1560
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: none
star: false
creator: Zhen Xu , Binjie Xu
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0015
component: BBa_C0261
sequence annotation: BBa_C0261
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K082014_sequence/1

BBa_B0015
type: Component
persistent identity: component2249441
display id: component2249441
version: 1
top level: BBa_K082014
definition: BBa_B0015
access: public

BBa_C0261
type: Component
persistent identity: component2249434
display id: component2249434
version: 1
top level: BBa_K082014
definition: BBa_C0261
access: public

BBa_C0261
type: SequenceAnnotation
persistent identity: annotation2249434
display id: annotation2249434
version: 1
top level: BBa_K082014
location: https://synbiohub.org/public/igem/BBa_K082014/annotation2249434/range2249434/1
component: BBa_C0261

https://synbiohub.org/public/igem/BBa_K082014/annotation2249434/range2249434/1
type: Range
persistent identity: range2249434
display id: range2249434
version: 1
top level: BBa_K082014
direction: direction/reverse
start: 1
end: 661
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2249441
display id: annotation2249441
version: 1
top level: BBa_K082014
location: https://synbiohub.org/public/igem/BBa_K082014/annotation2249441/range2249441/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K082014/annotation2249441/range2249441/1
type: Range
persistent identity: range2249441
display id: range2249441
version: 1
top level: BBa_K082014
direction: direction/reverse
start: 670
end: 798
orientation: inline

pLux/Tet
type: ComponentDefinition
persistent identity: BBa K176000
display id: BBa_K176000
version: 1
was generated by: iGEM to SBOL conversion
description: pLux/Tet Hybrid Promoter: (LuxR+,TetR-)->PoPS
created: 2009-05-05T11:00:00Z
modified: 2015-05-08T01:11:00Z
mutable provenance: Tet Operator sequence from BBa_R0040
owned by: james
owned by: myers
mutable description: sense TWO INPUTS, activation by AHL and repression by tetR
discontinued: false
dominant: false
experience: experience/Works
group u list: _282_
m user id: 0
owner id: 4010
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: How to arrange the two parts together?
star: false
creator: Danqian Liu, Chao Li, Hao Jiang
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: luxR/HSL
sequence annotation: R0062
sequence annotation: -35
sequence annotation: -10
sequence annotation: +1
sequence annotation: tetR
sequence: https://synbiohub.org/public/igem/BBa_K176000_sequence/1

luxR/HSL
type: SequenceAnnotation
persistent identity: annotation2002612
display id: annotation2002612
version: 1
top level: pLux/Tet
location: https://synbiohub.org/public/igem/BBa_K176000/annotation2002612/range2002612/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_K176000/annotation2002612/range2002612/1
type: Range
persistent identity: range2002612
display id: range2002612
version: 1
top level: pLux/Tet
direction: direction/forward
start: 1
end: 20
orientation: inline

R0062
type: SequenceAnnotation
persistent identity: annotation2002607
display id: annotation2002607
version: 1
top level: pLux/Tet
location: https://synbiohub.org/public/igem/BBa_K176000/annotation2002607/range2002607/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K176000/annotation2002607/range2002607/1
type: Range
persistent identity: range2002607
display id: range2002607
version: 1
top level: pLux/Tet
direction: direction/forward
start: 1
end: 53
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2002608
display id: annotation2002608
version: 1
top level: pLux/Tet
location: https://synbiohub.org/public/igem/BBa_K176000/annotation2002608/range2002608/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_K176000/annotation2002608/range2002608/1
type: Range
persistent identity: range2002608
display id: range2002608
version: 1
top level: pLux/Tet
direction: direction/forward
start: 20
end: 25
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2002609
display id: annotation2002609
version: 1
top level: pLux/Tet
location: https://synbiohub.org/public/igem/BBa_K176000/annotation2002609/range2002609/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_K176000/annotation2002609/range2002609/1
type: Range
persistent identity: range2002609
display id: range2002609
version: 1
top level: pLux/Tet
direction: direction/forward
start: 42
end: 47
orientation: inline

+1
type: SequenceAnnotation
persistent identity: annotation2002611
display id: annotation2002611
version: 1
top level: pLux/Tet
location: https://synbiohub.org/public/igem/BBa_K176000/annotation2002611/range2002611/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_K176000/annotation2002611/range2002611/1
type: Range
persistent identity: range2002611
display id: range2002611
version: 1
top level: pLux/Tet
direction: direction/forward
start: 53
end: 53
orientation: inline

tetR
type: SequenceAnnotation
persistent identity: annotation2002610
display id: annotation2002610
version: 1
top level: pLux/Tet
location: https://synbiohub.org/public/igem/BBa_K176000/annotation2002610/range2002610/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_K176000/annotation2002610/range2002610/1
type: Range
persistent identity: range2002610
display id: range2002610
version: 1
top level: pLux/Tet
direction: direction/forward
start: 54
end: 72
orientation: inline

LuxR
type: ComponentDefinition
persistent identity: BBa K176025
display id: BBa_K176025
version: 1
was generated by: iGEM to SBOL conversion
description: pCon 0.70(J23101)->RBS+luxR+T
created: 2009-07-15T11:00:00Z
modified: 2015-05-08T01:11:01Z
mutable provenance: J23101 I0462
owned by: james
owned by: myers
mutable description: pCon 0.70(J23101)+luxR(I0462)
discontinued: false
dominant: false
experience: experience/Works
group u list: _282_
m user id: 0
owner id: 3908
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: pCon 0.70(J23101) generate LuxR
star: false
creator: Danqian Liu, Chao Li, Hao Jiang
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_J23101
component: BBa_I0462
sequence annotation: BBa_J23101
sequence annotation: BBa_I0462
sequence: https://synbiohub.org/public/igem/BBa_K176025_sequence/1

BBa_J23101
type: Component
persistent identity: component2255337
display id: component2255337
version: 1
top level: LuxR
definition: BBa_J23101
access: public

BBa_I0462
type: Component
persistent identity: component2255352
display id: component2255352
version: 1
top level: LuxR
definition: LuxR
access: public

BBa_J23101
type: SequenceAnnotation
persistent identity: annotation2255337
display id: annotation2255337
version: 1
top level: LuxR
location: https://synbiohub.org/public/igem/BBa_K176025/annotation2255337/range2255337/1
component: BBa_J23101

https://synbiohub.org/public/igem/BBa_K176025/annotation2255337/range2255337/1
type: Range
persistent identity: range2255337
display id: range2255337
version: 1
top level: LuxR
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_I0462
type: SequenceAnnotation
persistent identity: annotation2255352
display id: annotation2255352
version: 1
top level: LuxR
location: https://synbiohub.org/public/igem/BBa_K176025/annotation2255352/range2255352/1
component: BBa_I0462

https://synbiohub.org/public/igem/BBa_K176025/annotation2255352/range2255352/1
type: Range
persistent identity: range2255352
display id: range2255352
version: 1
top level: LuxR
direction: direction/reverse
start: 44
end: 979
orientation: inline

BBa_C0261
type: ComponentDefinition
persistent identity: BBa C0261
display id: BBa_C0261
version: 1
was generated by: iGEM to SBOL conversion
description: AHL-making Enzyme, luxI (+RBS)
created: 2004-08-22T11:00:00Z
modified: 2015-08-31T04:07:24Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: B0034.C0061
discontinued: false
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 159
owning group id: 7
sample status: In stock
status: status/Available
bookmark: true
star: true
creator: robertb
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_C0061
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_C0061
sequence: https://synbiohub.org/public/igem/BBa_C0261_sequence/1

BBa_C0061
type: Component
persistent identity: component1060413
display id: component1060413
version: 1
top level: BBa_C0261
definition: luxI
access: public

BBa_B0034
type: Component
persistent identity: component1060403
display id: component1060403
version: 1
top level: BBa_C0261
definition: BBa_B0034
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1060403
display id: annotation1060403
version: 1
top level: BBa_C0261
location: https://synbiohub.org/public/igem/BBa_C0261/annotation1060403/range1060403/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_C0261/annotation1060403/range1060403/1
type: Range
persistent identity: range1060403
display id: range1060403
version: 1
top level: BBa_C0261
direction: direction/forward
start: 1
end: 12
orientation: inline

BBa_C0061
type: SequenceAnnotation
persistent identity: annotation1060413
display id: annotation1060413
version: 1
top level: BBa_C0261
location: https://synbiohub.org/public/igem/BBa_C0261/annotation1060413/range1060413/1
component: BBa_C0061

https://synbiohub.org/public/igem/BBa_C0261/annotation1060413/range1060413/1
type: Range
persistent identity: range1060413
display id: range1060413
version: 1
top level: BBa_C0261
direction: direction/forward
start: 19
end: 636
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

LuxR
type: ComponentDefinition
persistent identity: BBa I0462
display id: BBa_I0462
version: 1
was generated by: iGEM to SBOL conversion
description: luxR Protein Generator
created: 2003-12-04T12:00:00Z
modified: 2015-08-31T04:07:29Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Produces LuxR protein which can sense 3OC6HSL in the media and activate transcription from R0062, the right hand Lux promoter
discontinued: false
dominant: false
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Caitlin Conboy and Jennifer Braff
type: DnaRegion
role: partType/Generator
role: SO:0000804
component: BBa_B0034
component: BBa_B0010
component: BBa_C0062
component: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_C0062
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_I0462_sequence/1

BBa_B0034
type: Component
persistent identity: component943166
display id: component943166
version: 1
top level: LuxR
definition: BBa_B0034
access: public

BBa_B0010
type: Component
persistent identity: component943189
display id: component943189
version: 1
top level: LuxR
definition: BBa_B0010
access: public

BBa_C0062
type: Component
persistent identity: component943181
display id: component943181
version: 1
top level: LuxR
definition: luxr
access: public

BBa_B0012
type: Component
persistent identity: component943199
display id: component943199
version: 1
top level: LuxR
definition: BBa_B0012
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation943166
display id: annotation943166
version: 1
top level: LuxR
location: https://synbiohub.org/public/igem/BBa_I0462/annotation943166/range943166/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I0462/annotation943166/range943166/1
type: Range
persistent identity: range943166
display id: range943166
version: 1
top level: LuxR
direction: direction/forward
start: 1
end: 12
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation943181
display id: annotation943181
version: 1
top level: LuxR
location: https://synbiohub.org/public/igem/BBa_I0462/annotation943181/range943181/1
component: BBa_C0062

https://synbiohub.org/public/igem/BBa_I0462/annotation943181/range943181/1
type: Range
persistent identity: range943181
display id: range943181
version: 1
top level: LuxR
direction: direction/forward
start: 19
end: 774
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation943189
display id: annotation943189
version: 1
top level: LuxR
location: https://synbiohub.org/public/igem/BBa_I0462/annotation943189/range943189/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I0462/annotation943189/range943189/1
type: Range
persistent identity: range943189
display id: range943189
version: 1
top level: LuxR
direction: direction/forward
start: 808
end: 887
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation943199
display id: annotation943199
version: 1
top level: LuxR
location: https://synbiohub.org/public/igem/BBa_I0462/annotation943199/range943199/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I0462/annotation943199/range943199/1
type: Range
persistent identity: range943199
display id: range943199
version: 1
top level: LuxR
direction: direction/forward
start: 896
end: 936
orientation: inline

BBa_J23101
type: ComponentDefinition
persistent identity: BBa J23101
display id: BBa_J23101
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-03T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: later
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: later
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23101_sequence/1

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

luxI
type: ComponentDefinition
persistent identity: BBa C0061
display id: BBa_C0061
version: 1
was generated by: iGEM to SBOL conversion
description: autoinducer synthetase for AHL
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: V. fischeri <genbank>AF170104</genbank>
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Synthesizes 3OC6HSL, which binds to LuxR. The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the Lux repressor, LuxR. Two molecules of LuxR protein form a complex with two molecules the signalling compound HSL. This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: An LVA tail (sequence: AANDENYALVA) was added to increase protein degradation. .
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: BBa_C0061
sequence annotation: luxI
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0061_sequence/1

BBa_C0061
type: SequenceAnnotation
persistent identity: annotation7038
display id: annotation7038
version: 1
top level: luxI
location: https://synbiohub.org/public/igem/BBa_C0061/annotation7038/range7038/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_C0061/annotation7038/range7038/1
type: Range
persistent identity: range7038
display id: range7038
version: 1
top level: luxI
direction: direction/forward
start: 1
end: 618
orientation: inline

luxI
type: SequenceAnnotation
persistent identity: annotation1761
display id: annotation1761
version: 1
top level: luxI
location: https://synbiohub.org/public/igem/BBa_C0061/annotation1761/range1761/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_C0061/annotation1761/range1761/1
type: Range
persistent identity: range1761
display id: range1761
version: 1
top level: luxI
direction: direction/forward
start: 1
end: 579
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation1760
display id: annotation1760
version: 1
top level: luxI
location: https://synbiohub.org/public/igem/BBa_C0061/annotation1760/range1760/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_C0061/annotation1760/range1760/1
type: Range
persistent identity: range1760
display id: range1760
version: 1
top level: luxI
direction: direction/forward
start: 580
end: 611
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213985
display id: annotation2213985
version: 1
top level: luxI
location: https://synbiohub.org/public/igem/BBa_C0061/annotation2213985/range2213985/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_C0061/annotation2213985/range2213985/1
type: Range
persistent identity: range2213985
display id: range2213985
version: 1
top level: luxI
direction: direction/forward
start: 619
end: 643
orientation: inline

luxr
type: ComponentDefinition
persistent identity: BBa C0062
display id: BBa_C0062
version: 1
was generated by: iGEM to SBOL conversion
description: luxR repressor/activator, (no LVA?)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: V. fischeri <genbank>AF170104</genbank>
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: In complex with HSL, LuxR binds to the Lux promoter, activating transcription from Pr <bb_part>BBa_R0062</bb_part>, and repressing transcription from Pl <bb_part>BBa_R0063</bb_part>. The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the Lux activator, LuxR complexed to HSL. Two molecules of LuxR protein form a complex with two molecules the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: 2 silent point mutants were introduced in the coding sequence to remove internal XbaI and PstI sites. Mutation sites were chosen to replace codons commonly used in E. coli with codons used at a similar frequency.
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: luxR
sequence annotation: prefix
sequence annotation: BBa_C0062
sequence annotation: T
sequence annotation: A
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0062_sequence/1

luxR
type: SequenceAnnotation
persistent identity: annotation1766
display id: annotation1766
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1766/range1766/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_C0062/annotation1766/range1766/1
type: Range
persistent identity: range1766
display id: range1766
version: 1
top level: luxr
direction: direction/forward
start: 1
end: 750
orientation: inline

prefix
type: SequenceAnnotation
persistent identity: annotation1762
display id: annotation1762
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1762/range1762/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_C0062/annotation1762/range1762/1
type: Range
persistent identity: range1762
display id: range1762
version: 1
top level: luxr
direction: direction/forward
start: 1
end: 2
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation7039
display id: annotation7039
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation7039/range7039/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_C0062/annotation7039/range7039/1
type: Range
persistent identity: range7039
display id: range7039
version: 1
top level: luxr
direction: direction/forward
start: 1
end: 756
orientation: inline

T
type: SequenceAnnotation
persistent identity: annotation1764
display id: annotation1764
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1764/range1764/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_C0062/annotation1764/range1764/1
type: Range
persistent identity: range1764
display id: range1764
version: 1
top level: luxr
direction: direction/forward
start: 174
end: 174
orientation: inline

A
type: SequenceAnnotation
persistent identity: annotation1765
display id: annotation1765
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1765/range1765/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_C0062/annotation1765/range1765/1
type: Range
persistent identity: range1765
display id: range1765
version: 1
top level: luxr
direction: direction/forward
start: 492
end: 492
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213986
display id: annotation2213986
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation2213986/range2213986/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_C0062/annotation2213986/range2213986/1
type: Range
persistent identity: range2213986
display id: range2213986
version: 1
top level: luxr
direction: direction/forward
start: 757
end: 781
orientation: inline

https://synbiohub.org/public/igem/BBa_K176092_sequence/1
type: Sequence
persistent identity: BBa K176092 sequence
display id: BBa_K176092_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacagctagctcagtcctaggtataatgctagctactagagaaagaggagaaatactagatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtttacagctagctcagtcctaggtattatgctagctactagagaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaatatccctatcagtgatagagatactagagtcacacaggaaacctactagatgaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K176063_sequence/1
type: Sequence
persistent identity: BBa K176063 sequence
display id: BBa_K176063_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaacctactagatgaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctatacgtacggcagtttaaggtttacacctataaaagagagagccgttatcgtctgtttgtggatgtacagagtgatattattgacacgccggggcgacggatggtgatccccctggccagtgcacgtctgctgtcagataaagtctcccgtgaactttacccggtggtgcatatcggggatgaaagctggcgcatgatgaccaccgatatggccagtgtgccggtctccgttatcggggaagaagtggctgatctcagccaccgcgaaaatgacatcaaaaacgccattaacctgatgttctggggaatataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K176081_sequence/1
type: Sequence
persistent identity: BBa K176081 sequence
display id: BBa_K176081_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacagctagctcagtcctaggtataatgctagctactagagaaagaggagaaatactagatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtttacagctagctcagtcctaggtattatgctagctactagagaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaatatccctatcagtgatagaga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0031_sequence/1
type: Sequence
persistent identity: BBa B0031 sequence
display id: BBa_B0031_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaacc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K176018_sequence/1
type: Sequence
persistent identity: BBa K176018 sequence
display id: BBa_K176018_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacagctagctcagtcctaggtataatgctagctactagagaaagaggagaaatactagatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K176003_sequence/1
type: Sequence
persistent identity: BBa K176003 sequence
display id: BBa_K176003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctatacgtacggcagtttaaggtttacacctataaaagagagagccgttatcgtctgtttgtggatgtacagagtgatattattgacacgccggggcgacggatggtgatccccctggccagtgcacgtctgctgtcagataaagtctcccgtgaactttacccggtggtgcatatcggggatgaaagctggcgcatgatgaccaccgatatggccagtgtgccggtctccgttatcggggaagaagtggctgatctcagccaccgcgaaaatgacatcaaaaacgccattaacctgatgttctggggaatataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K176028_sequence/1
type: Sequence
persistent identity: BBa K176028 sequence
display id: BBa_K176028_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacagctagctcagtcctaggtattatgctagctactagagaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaatatccctatcagtgatagaga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23119_sequence/1
type: Sequence
persistent identity: BBa J23119 sequence
display id: BBa_J23119_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacagctagctcagtcctaggtataatgctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K082014_sequence/1
type: Sequence
persistent identity: BBa K082014 sequence
display id: BBa_K082014_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K176000_sequence/1
type: Sequence
persistent identity: BBa K176000 sequence
display id: BBa_K176000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaatatccctatcagtgatagaga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K176025_sequence/1
type: Sequence
persistent identity: BBa K176025 sequence
display id: BBa_K176025_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacagctagctcagtcctaggtattatgctagctactagagaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0261_sequence/1
type: Sequence
persistent identity: BBa C0261 sequence
display id: BBa_C0261_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I0462_sequence/1
type: Sequence
persistent identity: BBa I0462 sequence
display id: BBa_I0462_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23101_sequence/1
type: Sequence
persistent identity: BBa J23101 sequence
display id: BBa_J23101_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacagctagctcagtcctaggtattatgctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0061_sequence/1
type: Sequence
persistent identity: BBa C0061 sequence
display id: BBa_C0061_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0062_sequence/1
type: Sequence
persistent identity: BBa C0062 sequence
display id: BBa_C0062_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcac
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z