BBa_K1755303
type: ComponentDefinition
persistent identity: BBa K1755303
display id: BBa_K1755303
version: 1
was generated by: iGEM to SBOL conversion
description: cobalt sensor
created: 2015-04-19T11:00:00Z
modified: 2016-01-11T01:58:18Z
owned by: james
owned by: myers
mutable description: K540001 + K1755003
discontinued: false
dominant: false
group u list: _2177_
m user id: 4206
owner id: 21246
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Haotian Wang
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_K540001
component: BBa_K1755001
component: BBa_B0034
component: BBa_B0012
component: BBa_B0010
component: BBa_K1755003
sequence annotation: BBa_K540001
sequence annotation: BBa_K1755003
sequence annotation: BBa_B0034
sequence annotation: BBa_K1755001
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K1755303_sequence/1

BBa_K540001
type: Component
persistent identity: component2431317
display id: component2431317
version: 1
top level: BBa_K1755303
definition: BBa_K540001
access: public

BBa_K1755001
type: Component
persistent identity: component2431321
display id: component2431321
version: 1
top level: BBa_K1755303
definition: BBa_K1755001
access: public

BBa_B0034
type: Component
persistent identity: component2431318
display id: component2431318
version: 1
top level: BBa_K1755303
definition: BBa_B0034
access: public

BBa_B0012
type: Component
persistent identity: component2431325
display id: component2431325
version: 1
top level: BBa_K1755303
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component2431322
display id: component2431322
version: 1
top level: BBa_K1755303
definition: BBa_B0010
access: public

BBa_K1755003
type: Component
persistent identity: component2431329
display id: component2431329
version: 1
top level: BBa_K1755303
definition: BBa_K1755003
access: public

BBa_K540001
type: SequenceAnnotation
persistent identity: annotation2431317
display id: annotation2431317
version: 1
top level: BBa_K1755303
location: https://synbiohub.org/public/igem/BBa_K1755303/annotation2431317/range2431317/1
component: BBa_K540001

https://synbiohub.org/public/igem/BBa_K1755303/annotation2431317/range2431317/1
type: Range
persistent identity: range2431317
display id: range2431317
version: 1
top level: BBa_K1755303
direction: direction/reverse
start: 1
end: 426
orientation: inline

BBa_K1755003
type: SequenceAnnotation
persistent identity: annotation2431329
display id: annotation2431329
version: 1
top level: BBa_K1755303
location: https://synbiohub.org/public/igem/BBa_K1755303/annotation2431329/range2431329/1
component: BBa_K1755003

https://synbiohub.org/public/igem/BBa_K1755303/annotation2431329/range2431329/1
type: Range
persistent identity: range2431329
display id: range2431329
version: 1
top level: BBa_K1755303
direction: direction/reverse
start: 435
end: 1243
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2431318
display id: annotation2431318
version: 1
top level: BBa_K1755303
location: https://synbiohub.org/public/igem/BBa_K1755303/annotation2431318/range2431318/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1755303/annotation2431318/range2431318/1
type: Range
persistent identity: range2431318
display id: range2431318
version: 1
top level: BBa_K1755303
direction: direction/reverse
start: 435
end: 446
orientation: inline

BBa_K1755001
type: SequenceAnnotation
persistent identity: annotation2431321
display id: annotation2431321
version: 1
top level: BBa_K1755303
location: https://synbiohub.org/public/igem/BBa_K1755303/annotation2431321/range2431321/1
component: BBa_K1755001

https://synbiohub.org/public/igem/BBa_K1755303/annotation2431321/range2431321/1
type: Range
persistent identity: range2431321
display id: range2431321
version: 1
top level: BBa_K1755303
direction: direction/reverse
start: 453
end: 1106
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2431322
display id: annotation2431322
version: 1
top level: BBa_K1755303
location: https://synbiohub.org/public/igem/BBa_K1755303/annotation2431322/range2431322/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K1755303/annotation2431322/range2431322/1
type: Range
persistent identity: range2431322
display id: range2431322
version: 1
top level: BBa_K1755303
direction: direction/forward
start: 1115
end: 1194
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2431325
display id: annotation2431325
version: 1
top level: BBa_K1755303
location: https://synbiohub.org/public/igem/BBa_K1755303/annotation2431325/range2431325/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K1755303/annotation2431325/range2431325/1
type: Range
persistent identity: range2431325
display id: range2431325
version: 1
top level: BBa_K1755303
direction: direction/forward
start: 1203
end: 1243
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

BBa_K1755001
type: ComponentDefinition
persistent identity: BBa K1755001
display id: BBa_K1755001
version: 1
was generated by: iGEM to SBOL conversion
description: ribB coding sequence
created: 2015-04-19T11:00:00Z
modified: 2015-05-08T01:11:00Z
mutable provenance: You can find it from almost all E.coli cells. More infomation you can find from this website: http://www.ncbi.nlm.nih.gov/gene/12931678
owned by: james
owned by: myers
mutable description: ribB，the 3,4 dihydroxy-2-butanone-4-phosphate synthase。 We get this part from E.coli genome by PCR. It can increase the electric output by synthesis of riboflavin.
discontinued: false
dominant: false
group u list: _2177_
m user id: 0
owner id: 21246
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: The primer is below: F:5'-3' CGT TCTAGA TGAATCAGACGCT 60.46 45 22 R:5'-3' GGT ACTAGT TCAGCTGGCTTAC 60.66 50 22
star: false
creator: Haotian Wang
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1755001_sequence/1

BBa_K1755003
type: ComponentDefinition
persistent identity: BBa K1755003
display id: BBa_K1755003
version: 1
was generated by: iGEM to SBOL conversion
description: strong RBS + K1755002
created: 2015-04-19T11:00:00Z
modified: 2015-06-01T08:47:09Z
owned by: james
owned by: myers
mutable description: B0034+ribB+B0015
discontinued: false
dominant: false
group u list: _2177_
m user id: 21246
owner id: 21246
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Haotian Wang
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0032
component: BBa_K1755002
sequence annotation: BBa_B0032
sequence annotation: BBa_K1755002
sequence: https://synbiohub.org/public/igem/BBa_K1755003_sequence/1

BBa_B0032
type: Component
persistent identity: component2432415
display id: component2432415
version: 1
top level: BBa_K1755003
definition: BBa_B0032
access: public

BBa_K1755002
type: Component
persistent identity: component2432425
display id: component2432425
version: 1
top level: BBa_K1755003
definition: BBa_K1755002
access: public

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2432415
display id: annotation2432415
version: 1
top level: BBa_K1755003
location: https://synbiohub.org/public/igem/BBa_K1755003/annotation2432415/range2432415/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K1755003/annotation2432415/range2432415/1
type: Range
persistent identity: range2432415
display id: range2432415
version: 1
top level: BBa_K1755003
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_K1755002
type: SequenceAnnotation
persistent identity: annotation2432425
display id: annotation2432425
version: 1
top level: BBa_K1755003
location: https://synbiohub.org/public/igem/BBa_K1755003/annotation2432425/range2432425/1
component: BBa_K1755002

https://synbiohub.org/public/igem/BBa_K1755003/annotation2432425/range2432425/1
type: Range
persistent identity: range2432425
display id: range2432425
version: 1
top level: BBa_K1755003
direction: direction/reverse
start: 20
end: 776
orientation: inline

BBa_K540001
type: ComponentDefinition
persistent identity: BBa K540001
display id: BBa_K540001
version: 1
was generated by: iGEM to SBOL conversion
description: rcn, cobalt-sensitive promoter
created: 2011-09-14T11:00:00Z
modified: 2015-07-14T12:34:23Z
mutable provenance: PCR from E.coli MC4100 genome.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Rcn is a promoter that is activated by cobalt. A repressor is fixed to the promoter by default, and cobalt binds to this repressor, which activates the gene under control of this promoter. Rcn is also activated by nickel, but we have not characterized this aspect.
discontinued: false
dominant: false
experience: experience/Works
group u list: _706_
m user id: 4206
owner id: 8419
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: An internal iGEM restriction site had to be mutated by QuickChange mutagenesis.
star: false
creator: Mathilde Dumond
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: RcnR
sequence: https://synbiohub.org/public/igem/BBa_K540001_sequence/1

RcnR
type: SequenceAnnotation
persistent identity: annotation2129035
display id: annotation2129035
version: 1
top level: BBa_K540001
location: https://synbiohub.org/public/igem/BBa_K540001/annotation2129035/range2129035/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K540001/annotation2129035/range2129035/1
type: Range
persistent identity: range2129035
display id: range2129035
version: 1
top level: BBa_K540001
direction: direction/reverse
start: 1
end: 279
orientation: inline

BBa_B0032
type: ComponentDefinition
persistent identity: BBa B0032
display id: BBa_B0032
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.3 (medium) -- derivative of BBa_0030
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: RBS-3\Weak
sequence annotation: BBa_B0032
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0032_sequence/1

RBS-3\Weak
type: SequenceAnnotation
persistent identity: annotation1709
display id: annotation1709
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
type: Range
persistent identity: range1709
display id: range1709
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation7027
display id: annotation7027
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
type: Range
persistent identity: range7027
display id: range7027
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1710
display id: annotation1710
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
type: Range
persistent identity: range1710
display id: range1710
version: 1
top level: BBa_B0032
direction: direction/forward
start: 7
end: 10
orientation: inline

BBa_K1755002
type: ComponentDefinition
persistent identity: BBa K1755002
display id: BBa_K1755002
version: 1
was generated by: iGEM to SBOL conversion
description: ribB+B0015
created: 2015-04-19T11:00:00Z
modified: 2015-06-01T08:38:11Z
owned by: james
owned by: myers
mutable description: ribB with double terminators
discontinued: false
dominant: false
group u list: _2177_
m user id: 21246
owner id: 21246
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Haotian Wang
type: DnaRegion
role: SO:0000316
role: partType/Coding
component: BBa_B0014
component: BBa_K1755001
sequence annotation: BBa_K1755001
sequence annotation: BBa_B0014
sequence: https://synbiohub.org/public/igem/BBa_K1755002_sequence/1

BBa_B0014
type: Component
persistent identity: component2432413
display id: component2432413
version: 1
top level: BBa_K1755002
definition: BBa_B0014
access: public

BBa_K1755001
type: Component
persistent identity: component2432406
display id: component2432406
version: 1
top level: BBa_K1755002
definition: BBa_K1755001
access: public

BBa_K1755001
type: SequenceAnnotation
persistent identity: annotation2432406
display id: annotation2432406
version: 1
top level: BBa_K1755002
location: https://synbiohub.org/public/igem/BBa_K1755002/annotation2432406/range2432406/1
component: BBa_K1755001

https://synbiohub.org/public/igem/BBa_K1755002/annotation2432406/range2432406/1
type: Range
persistent identity: range2432406
display id: range2432406
version: 1
top level: BBa_K1755002
direction: direction/reverse
start: 1
end: 654
orientation: inline

BBa_B0014
type: SequenceAnnotation
persistent identity: annotation2432413
display id: annotation2432413
version: 1
top level: BBa_K1755002
location: https://synbiohub.org/public/igem/BBa_K1755002/annotation2432413/range2432413/1
component: BBa_B0014

https://synbiohub.org/public/igem/BBa_K1755002/annotation2432413/range2432413/1
type: Range
persistent identity: range2432413
display id: range2432413
version: 1
top level: BBa_K1755002
direction: direction/reverse
start: 663
end: 757
orientation: inline

BBa_B0014
type: ComponentDefinition
persistent identity: BBa B0014
display id: BBa_B0014
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0012-B0011)
created: 2003-07-15T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0012 and BBa_B0011
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
component: BBa_B0011
component: BBa_B0012
sequence annotation: BBa_B0012
sequence annotation: BBa_B0011
sequence: https://synbiohub.org/public/igem/BBa_B0014_sequence/1

BBa_B0011
type: Component
persistent identity: component939311
display id: component939311
version: 1
top level: BBa_B0014
definition: BBa_B0011
access: public

BBa_B0012
type: Component
persistent identity: component939303
display id: component939303
version: 1
top level: BBa_B0014
definition: BBa_B0012
access: public

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation939303
display id: annotation939303
version: 1
top level: BBa_B0014
location: https://synbiohub.org/public/igem/BBa_B0014/annotation939303/range939303/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0014/annotation939303/range939303/1
type: Range
persistent identity: range939303
display id: range939303
version: 1
top level: BBa_B0014
direction: direction/forward
start: 1
end: 41
orientation: inline

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation939311
display id: annotation939311
version: 1
top level: BBa_B0014
location: https://synbiohub.org/public/igem/BBa_B0014/annotation939311/range939311/1
component: BBa_B0011

https://synbiohub.org/public/igem/BBa_B0014/annotation939311/range939311/1
type: Range
persistent identity: range939311
display id: range939311
version: 1
top level: BBa_B0014
direction: direction/forward
start: 50
end: 95
orientation: inline

BBa_B0011
type: ComponentDefinition
persistent identity: BBa B0011
display id: BBa_B0011
version: 1
was generated by: iGEM to SBOL conversion
description: LuxICDABEG (+/-)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from luxICDABEG operon terminator of Vibrio fischeri <genbank>AF170104</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Bidirectional transcriptional terminator consisting of a 22 bp stem-loop.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: In the naturally-occuring sequence there is a mismatch in the stem of the stem loop. This can be corrected via an A->G mutation (at position 40 -- sequence coordinate/not MFOLD coordinate). The above sequence does not reflect this mutation (but the MFOLD image does). This terminator's location cannot be found using some inverted repeat detectors like PALINDROME because it is too short and contains a mismatch. This one was found with the help of Tom Knight. It lies between two coding regions that point towards eachother.
star: true
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0011
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0011_sequence/1

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation7019
display id: annotation7019
version: 1
top level: BBa_B0011
location: https://synbiohub.org/public/igem/BBa_B0011/annotation7019/range7019/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0011/annotation7019/range7019/1
type: Range
persistent identity: range7019
display id: range7019
version: 1
top level: BBa_B0011
direction: direction/forward
start: 1
end: 46
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation1683
display id: annotation1683
version: 1
top level: BBa_B0011
location: https://synbiohub.org/public/igem/BBa_B0011/annotation1683/range1683/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0011/annotation1683/range1683/1
type: Range
persistent identity: range1683
display id: range1683
version: 1
top level: BBa_B0011
direction: direction/forward
start: 13
end: 35
orientation: inline

https://synbiohub.org/public/igem/BBa_K1755303_sequence/1
type: Sequence
persistent identity: BBa K1755303 sequence
display id: BBa_K1755303_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttattattatttgatatatgaatccagcaccttcagaacgacatccagatcttcttcacgttttagctcatccccctggtgaacgatgtgttccgtcagatgacctttaatcacttcccgcatcagaccgtttaccgcgccacggatagcagcaatctgttgtaaaactgctgcgcattcgtgcggctcgtcgagcattttcttgagcgccacgacctggccctgaatcttactggcacgcgctttcagtttctgtttatcacggattgtatgagacatggcaacacctggttaacaagaatatgaaaaatcatagcactattaatctactggggggtagtatcaggtactgggggggagtagaatcagattgccgaattaatactaagaattattatcatgaccgaatttacaactcttcttcagtactagagaaagaggagaaatactagatgaatcagacgctactttcctcttttggtacgcctttcgaacgtgttgaaaatgcactggctgcgctgcgtgaaggacgcggtgtaatggtgcttgatgatgaagaccgtgaaaacgaaggtgatatgatcttcccggcagaaaccatgactgttgagcagatggcgctgaccattcgccacggtagcggtattgtttgcctgtgcattactgaagatcgccgtaaacaactcgatctgccaatgatggtagaaaataacaccagcgcctatggcaccggttttaccgtgaccattgaagcagctgaaggtgtgactaccggtgtttctgccgctgaccgtattacgaccgttcgcgcagcgattgccgatggcgcaaaaccgtcagatctgaatcgtcctggccacgttttcccacttcgcgctcaggcaggtggtgtactgacgcgtggcggtcatactgaagcaactattgatctgatgacgctggcaggctttaaaccggctggtgtactgtgtgagctgactaatgacgatggcacgatggcgcgtgcaccagagtgtattgagtttgccaataaacacaatatggcgctcgtgactattgaagacctggtggcataccgtcaggcacatgagcgtaaagccagctgatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1755001_sequence/1
type: Sequence
persistent identity: BBa K1755001 sequence
display id: BBa_K1755001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaatcagacgctactttcctcttttggtacgcctttcgaacgtgttgaaaatgcactggctgcgctgcgtgaaggacgcggtgtaatggtgcttgatgatgaagaccgtgaaaacgaaggtgatatgatcttcccggcagaaaccatgactgttgagcagatggcgctgaccattcgccacggtagcggtattgtttgcctgtgcattactgaagatcgccgtaaacaactcgatctgccaatgatggtagaaaataacaccagcgcctatggcaccggttttaccgtgaccattgaagcagctgaaggtgtgactaccggtgtttctgccgctgaccgtattacgaccgttcgcgcagcgattgccgatggcgcaaaaccgtcagatctgaatcgtcctggccacgttttcccacttcgcgctcaggcaggtggtgtactgacgcgtggcggtcatactgaagcaactattgatctgatgacgctggcaggctttaaaccggctggtgtactgtgtgagctgactaatgacgatggcacgatggcgcgtgcaccagagtgtattgagtttgccaataaacacaatatggcgctcgtgactattgaagacctggtggcataccgtcaggcacatgagcgtaaagccagctga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1755003_sequence/1
type: Sequence
persistent identity: BBa K1755003 sequence
display id: BBa_K1755003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaagtactagatgaatcagacgctactttcctcttttggtacgcctttcgaacgtgttgaaaatgcactggctgcgctgcgtgaaggacgcggtgtaatggtgcttgatgatgaagaccgtgaaaacgaaggtgatatgatcttcccggcagaaaccatgactgttgagcagatggcgctgaccattcgccacggtagcggtattgtttgcctgtgcattactgaagatcgccgtaaacaactcgatctgccaatgatggtagaaaataacaccagcgcctatggcaccggttttaccgtgaccattgaagcagctgaaggtgtgactaccggtgtttctgccgctgaccgtattacgaccgttcgcgcagcgattgccgatggcgcaaaaccgtcagatctgaatcgtcctggccacgttttcccacttcgcgctcaggcaggtggtgtactgacgcgtggcggtcatactgaagcaactattgatctgatgacgctggcaggctttaaaccggctggtgtactgtgtgagctgactaatgacgatggcacgatggcgcgtgcaccagagtgtattgagtttgccaataaacacaatatggcgctcgtgactattgaagacctggtggcataccgtcaggcacatgagcgtaaagccagctgatactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K540001_sequence/1
type: Sequence
persistent identity: BBa K540001 sequence
display id: BBa_K540001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttattattatttgatatatgaatccagcaccttcagaacgacatccagatcttcttcacgttttagctcatccccctggtgaacgatgtgttccgtcagatgacctttaatcacttcccgcatcagaccgtttaccgcgccacggatagcagcaatctgttgtaaaactgctgcgcattcgtgcggctcgtcgagcattttcttgagcgccacgacctggccctgaatcttactggcacgcgctttcagtttctgtttatcacggattgtatgagacatggcaacacctggttaacaagaatatgaaaaatcatagcactattaatctactggggggtagtatcaggtactgggggggagtagaatcagattgccgaattaatactaagaattattatcatgaccgaatttacaactcttcttcag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0032_sequence/1
type: Sequence
persistent identity: BBa B0032 sequence
display id: BBa_B0032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1755002_sequence/1
type: Sequence
persistent identity: BBa K1755002 sequence
display id: BBa_K1755002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: myers
owned by: james
elements: atgaatcagacgctactttcctcttttggtacgcctttcgaacgtgttgaaaatgcactggctgcgctgcgtgaaggacgcggtgtaatggtgcttgatgatgaagaccgtgaaaacgaaggtgatatgatcttcccggcagaaaccatgactgttgagcagatggcgctgaccattcgccacggtagcggtattgtttgcctgtgcattactgaagatcgccgtaaacaactcgatctgccaatgatggtagaaaataacaccagcgcctatggcaccggttttaccgtgaccattgaagcagctgaaggtgtgactaccggtgtttctgccgctgaccgtattacgaccgttcgcgcagcgattgccgatggcgcaaaaccgtcagatctgaatcgtcctggccacgttttcccacttcgcgctcaggcaggtggtgtactgacgcgtggcggtcatactgaagcaactattgatctgatgacgctggcaggctttaaaccggctggtgtactgtgtgagctgactaatgacgatggcacgatggcgcgtgcaccagagtgtattgagtttgccaataaacacaatatggcgctcgtgactattgaagacctggtggcataccgtcaggcacatgagcgtaaagccagctgatactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0014_sequence/1
type: Sequence
persistent identity: BBa B0014 sequence
display id: BBa_B0014_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0011_sequence/1
type: Sequence
persistent identity: BBa B0011 sequence
display id: BBa_B0011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agagaatataaaaagccagattattaatccggcttttttattattt
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z