BBa_K1725103
type: ComponentDefinition
persistent identity: BBa K1725103
display id: BBa_K1725103
version: 1
was generated by: iGEM to SBOL conversion
description: SrpR repressible promoter + strong RBS + RFP + terminator + PhlF repressible promoter + weak RBS + G
created: 2015-09-09T11:00:00Z
modified: 2015-09-18T07:38:53Z
mutable provenance: .
owned by: james
owned by: myers
mutable description: .
discontinued: false
dominant: false
experience: experience/None
group u list: _2146_
m user id: 27310
owner id: 27284
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: .
star: false
creator: Mhairi Davidson
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_K1725002
component: BBa_K1725102
sequence annotation: BBa_K1725102
sequence annotation: BBa_K1725002
sequence: https://synbiohub.org/public/igem/BBa_K1725103_sequence/1

BBa_K1725002
type: Component
persistent identity: component2453147
display id: component2453147
version: 1
top level: BBa_K1725103
definition: BBa_K1725002
access: public

BBa_K1725102
type: Component
persistent identity: component2453140
display id: component2453140
version: 1
top level: BBa_K1725103
definition: BBa_K1725102
access: public

BBa_K1725102
type: SequenceAnnotation
persistent identity: annotation2453140
display id: annotation2453140
version: 1
top level: BBa_K1725103
location: https://synbiohub.org/public/igem/BBa_K1725103/annotation2453140/range2453140/1
component: BBa_K1725102

https://synbiohub.org/public/igem/BBa_K1725103/annotation2453140/range2453140/1
type: Range
persistent identity: range2453140
display id: range2453140
version: 1
top level: BBa_K1725103
direction: direction/reverse
start: 1
end: 939
orientation: inline

BBa_K1725002
type: SequenceAnnotation
persistent identity: annotation2453147
display id: annotation2453147
version: 1
top level: BBa_K1725103
location: https://synbiohub.org/public/igem/BBa_K1725103/annotation2453147/range2453147/1
component: BBa_K1725002

https://synbiohub.org/public/igem/BBa_K1725103/annotation2453147/range2453147/1
type: Range
persistent identity: range2453147
display id: range2453147
version: 1
top level: BBa_K1725103
direction: direction/reverse
start: 948
end: 1742
orientation: inline

BBa_K1725102
type: ComponentDefinition
persistent identity: BBa K1725102
display id: BBa_K1725102
version: 1
was generated by: iGEM to SBOL conversion
description: SrpR repressible promoter + strong RBS + RFP + terminator
created: 2015-09-09T11:00:00Z
modified: 2015-09-18T07:28:28Z
mutable provenance: .
owned by: james
owned by: myers
mutable description: .
discontinued: false
dominant: false
experience: experience/None
group u list: _2146_
m user id: 27310
owner id: 27284
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: .
star: false
creator: Mhairi Davidson
type: DnaRegion
role: SO:0000804
role: partType/Reporter
component: BBa_K516030
component: BBa_K1725020
sequence annotation: BBa_K1725020
sequence annotation: BBa_K516030
sequence: https://synbiohub.org/public/igem/BBa_K1725102_sequence/1

BBa_K516030
type: Component
persistent identity: component2453100
display id: component2453100
version: 1
top level: BBa_K1725102
definition: BBa_K516030
access: public

BBa_K1725020
type: Component
persistent identity: component2453086
display id: component2453086
version: 1
top level: BBa_K1725102
definition: P-srpR
access: public

BBa_K1725020
type: SequenceAnnotation
persistent identity: annotation2453086
display id: annotation2453086
version: 1
top level: BBa_K1725102
location: https://synbiohub.org/public/igem/BBa_K1725102/annotation2453086/range2453086/1
component: BBa_K1725020

https://synbiohub.org/public/igem/BBa_K1725102/annotation2453086/range2453086/1
type: Range
persistent identity: range2453086
display id: range2453086
version: 1
top level: BBa_K1725102
direction: direction/reverse
start: 1
end: 67
orientation: inline

BBa_K516030
type: SequenceAnnotation
persistent identity: annotation2453100
display id: annotation2453100
version: 1
top level: BBa_K1725102
location: https://synbiohub.org/public/igem/BBa_K1725102/annotation2453100/range2453100/1
component: BBa_K516030

https://synbiohub.org/public/igem/BBa_K1725102/annotation2453100/range2453100/1
type: Range
persistent identity: range2453100
display id: range2453100
version: 1
top level: BBa_K1725102
direction: direction/reverse
start: 76
end: 939
orientation: inline

BBa_K1725002
type: ComponentDefinition
persistent identity: BBa K1725002
display id: BBa_K1725002
version: 1
was generated by: iGEM to SBOL conversion
description: PhlF repressible promoter + weak RBS + GFP
created: 2015-08-10T11:00:00Z
modified: 2016-07-23T11:17:58Z
mutable provenance: PhlF promoter from "Genomic Mining of Prokaryotic Repressors for Orthogonal Logic Gates" Stanton et al http://www.nature.com/nchembio/journal/v10/n2/full/nchembio.1411.html
owned by: james
owned by: myers
mutable description: The promoter is on normally, and off when the PhlF repressor (K1725041) is bound to it. We measured the strength of the promoter with GFP and two different ribosome binding sites (B0032 and B0034), and compared to R0040 and K1725020.
discontinued: false
dominant: false
experience: experience/Works
group u list: _2146_
m user id: 27285
owner id: 27284
owning group id: 9
status: status/Planning
bookmark: true
mutable notes: .
star: false
creator: Mhairi Davidson
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_E5501
component: BBa_K1725000
sequence annotation: BBa_K1725000
sequence annotation: BBa_E5501
sequence: https://synbiohub.org/public/igem/BBa_K1725002_sequence/1

BBa_E5501
type: Component
persistent identity: component2435050
display id: component2435050
version: 1
top level: BBa_K1725002
definition: BBa_E5501
access: public

BBa_K1725000
type: Component
persistent identity: component2435045
display id: component2435045
version: 1
top level: BBa_K1725002
definition: P-phlF
access: public

BBa_K1725000
type: SequenceAnnotation
persistent identity: annotation2435045
display id: annotation2435045
version: 1
top level: BBa_K1725002
location: https://synbiohub.org/public/igem/BBa_K1725002/annotation2435045/range2435045/1
component: BBa_K1725000

https://synbiohub.org/public/igem/BBa_K1725002/annotation2435045/range2435045/1
type: Range
persistent identity: range2435045
display id: range2435045
version: 1
top level: BBa_K1725002
direction: direction/reverse
start: 1
end: 48
orientation: inline

BBa_E5501
type: SequenceAnnotation
persistent identity: annotation2435050
display id: annotation2435050
version: 1
top level: BBa_K1725002
location: https://synbiohub.org/public/igem/BBa_K1725002/annotation2435050/range2435050/1
component: BBa_E5501

https://synbiohub.org/public/igem/BBa_K1725002/annotation2435050/range2435050/1
type: Range
persistent identity: range2435050
display id: range2435050
version: 1
top level: BBa_K1725002
direction: direction/reverse
start: 57
end: 795
orientation: inline

P-phlF
type: ComponentDefinition
persistent identity: BBa K1725000
display id: BBa_K1725000
version: 1
was generated by: iGEM to SBOL conversion
description: PhlF repressible promoter
created: 2015-08-05T11:00:00Z
modified: 2016-07-23T11:16:25Z
mutable provenance: From "Genomic Mining of Prokaryotic Repressors for Orthogonal Logic Gates" Stanton et al http://www.nature.com/nchembio/journal/v10/n2/full/nchembio.1411.html
owned by: james
owned by: myers
mutable description: The promoter is on normally, and off when the PhlF repressor (K1725041) is bound to it. We measured the strength of the promoter with GFP and two different ribosome binding sites (B0032 and B0034), and compared to R0040 and K1725020.
discontinued: false
dominant: false
experience: experience/Works
group u list: _2146_
m user id: 27285
owner id: 27284
owning group id: 9
status: status/Planning
bookmark: true
mutable notes: We used the promotor sequence from "Genomic Mining of Prokaryotic Repressors for Orthogonal Logic Gates" Stanton et al http://www.nature.com/nchembio/journal/v10/n2/full/nchembio.1411.html They based the promoter sequence on J23119, then overlapped the operator sequence of the PhlF repressor over the -10 site.
star: false
creator: Mhairi Davidson
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35 site
sequence annotation: K1725040 operator sequence
sequence annotation: -10 site
sequence: https://synbiohub.org/public/igem/BBa_K1725000_sequence/1

-35 site
type: SequenceAnnotation
persistent identity: annotation2476166
display id: annotation2476166
version: 1
top level: P-phlF
location: https://synbiohub.org/public/igem/BBa_K1725000/annotation2476166/range2476166/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_K1725000/annotation2476166/range2476166/1
type: Range
persistent identity: range2476166
display id: range2476166
version: 1
top level: P-phlF
direction: direction/forward
start: 14
end: 19
orientation: inline

K1725040 operator sequence
type: SequenceAnnotation
persistent identity: annotation2461855
display id: annotation2461855
version: 1
top level: P-phlF
location: https://synbiohub.org/public/igem/BBa_K1725000/annotation2461855/range2461855/1
role: feature/operator
role: SO:0000057

https://synbiohub.org/public/igem/BBa_K1725000/annotation2461855/range2461855/1
type: Range
persistent identity: range2461855
display id: range2461855
version: 1
top level: P-phlF
direction: direction/forward
start: 19
end: 48
orientation: inline

-10 site
type: SequenceAnnotation
persistent identity: annotation2476167
display id: annotation2476167
version: 1
top level: P-phlF
location: https://synbiohub.org/public/igem/BBa_K1725000/annotation2476167/range2476167/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_K1725000/annotation2476167/range2476167/1
type: Range
persistent identity: range2476167
display id: range2476167
version: 1
top level: P-phlF
direction: direction/forward
start: 37
end: 42
orientation: inline

P-srpR
type: ComponentDefinition
persistent identity: BBa K1725020
display id: BBa_K1725020
version: 1
was generated by: iGEM to SBOL conversion
description: SrpR repressible promoter
created: 2015-08-06T11:00:00Z
modified: 2015-09-18T03:58:50Z
mutable provenance: From "Genomic Mining of Prokaryotic Repressors for Orthogonal Logic Gates" Stanton et al http://www.nature.com/nchembio/journal/v10/n2/full/nchembio.1411.html
owned by: james
owned by: myers
mutable description: The promoter is on normally, and off when the SrpR repressor (K1725061) is bound to it. We measured the strength of the promoter with GFP and two different ribosome binding sites (B0032 and B0034), and compared to R0040 and K1725000.
discontinued: false
dominant: false
experience: experience/None
group u list: _2146_
m user id: 20827
owner id: 27284
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: We used the promotor sequence from "Genomic Mining of Prokaryotic Repressors for Orthogonal Logic Gates" Stanton et al http://www.nature.com/nchembio/journal/v10/n2/full/nchembio.1411.html They based the promoter sequence on J23119, then overlapped the operator sequence of the SrpR repressor over the -10 site.
star: false
creator: Mhairi Davidson
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: K1725060 operator sequence
sequence: https://synbiohub.org/public/igem/BBa_K1725020_sequence/1

K1725060 operator sequence
type: SequenceAnnotation
persistent identity: annotation2461859
display id: annotation2461859
version: 1
top level: P-srpR
location: https://synbiohub.org/public/igem/BBa_K1725020/annotation2461859/range2461859/1
role: feature/operator
role: SO:0000057

https://synbiohub.org/public/igem/BBa_K1725020/annotation2461859/range2461859/1
type: Range
persistent identity: range2461859
display id: range2461859
version: 1
top level: P-srpR
direction: direction/forward
start: 38
end: 67
orientation: inline

BBa_E5501
type: ComponentDefinition
persistent identity: BBa E5501
display id: BBa_E5501
version: 1
was generated by: iGEM to SBOL conversion
description: B0032.E0040
created: 2005-05-16T11:00:00Z
modified: 2015-08-31T04:07:27Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: B0032.E0040
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 61
owning group id: 1
sample status: In stock
status: status/Available
bookmark: true
star: false
creator: jcbraff
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_B0032
component: BBa_E0040
sequence annotation: BBa_B0032
sequence annotation: BBa_E0040
sequence: https://synbiohub.org/public/igem/BBa_E5501_sequence/1

BBa_B0032
type: Component
persistent identity: component1489098
display id: component1489098
version: 1
top level: BBa_E5501
definition: BBa_B0032
access: public

BBa_E0040
type: Component
persistent identity: component1489101
display id: component1489101
version: 1
top level: BBa_E5501
definition: GFP
access: public

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1489098
display id: annotation1489098
version: 1
top level: BBa_E5501
location: https://synbiohub.org/public/igem/BBa_E5501/annotation1489098/range1489098/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_E5501/annotation1489098/range1489098/1
type: Range
persistent identity: range1489098
display id: range1489098
version: 1
top level: BBa_E5501
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1489101
display id: annotation1489101
version: 1
top level: BBa_E5501
location: https://synbiohub.org/public/igem/BBa_E5501/annotation1489101/range1489101/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_E5501/annotation1489101/range1489101/1
type: Range
persistent identity: range1489101
display id: range1489101
version: 1
top level: BBa_E5501
direction: direction/forward
start: 20
end: 739
orientation: inline

BBa_K516030
type: ComponentDefinition
persistent identity: BBa K516030
display id: BBa_K516030
version: 1
was generated by: iGEM to SBOL conversion
description: mRFP protein generator TERM+ RBS+(B0030)
created: 2011-09-07T11:00:00Z
modified: 2015-05-08T01:12:32Z
mutable provenance: Registry
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: mRFP protein generator TERM+ RBS+(B0030)
discontinued: false
dominant: false
experience: experience/Works
group u list: _681_
m user id: 0
owner id: 4150
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: --
star: false
creator: Nicolo' Politi, Federica Sampietro, Davide Bianchini
type: DnaRegion
role: SO:0000804
role: partType/Reporter
component: BBa_B0015
component: BBa_E1010
component: BBa_B0030
sequence annotation: BBa_B0030
sequence annotation: BBa_E1010
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K516030_sequence/1

BBa_B0015
type: Component
persistent identity: component2250583
display id: component2250583
version: 1
top level: BBa_K516030
definition: BBa_B0015
access: public

BBa_E1010
type: Component
persistent identity: component2250576
display id: component2250576
version: 1
top level: BBa_K516030
definition: mRFP1
access: public

BBa_B0030
type: Component
persistent identity: component2250572
display id: component2250572
version: 1
top level: BBa_K516030
definition: BBa_B0030
access: public

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2250572
display id: annotation2250572
version: 1
top level: BBa_K516030
location: https://synbiohub.org/public/igem/BBa_K516030/annotation2250572/range2250572/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K516030/annotation2250572/range2250572/1
type: Range
persistent identity: range2250572
display id: range2250572
version: 1
top level: BBa_K516030
direction: direction/forward
start: 1
end: 15
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2250576
display id: annotation2250576
version: 1
top level: BBa_K516030
location: https://synbiohub.org/public/igem/BBa_K516030/annotation2250576/range2250576/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_K516030/annotation2250576/range2250576/1
type: Range
persistent identity: range2250576
display id: range2250576
version: 1
top level: BBa_K516030
direction: direction/reverse
start: 22
end: 727
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2250583
display id: annotation2250583
version: 1
top level: BBa_K516030
location: https://synbiohub.org/public/igem/BBa_K516030/annotation2250583/range2250583/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K516030/annotation2250583/range2250583/1
type: Range
persistent identity: range2250583
display id: range2250583
version: 1
top level: BBa_K516030
direction: direction/reverse
start: 736
end: 864
orientation: inline

BBa_B0032
type: ComponentDefinition
persistent identity: BBa B0032
display id: BBa_B0032
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.3 (medium) -- derivative of BBa_0030
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: RBS-3\Weak
sequence annotation: BBa_B0032
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0032_sequence/1

RBS-3\Weak
type: SequenceAnnotation
persistent identity: annotation1709
display id: annotation1709
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
type: Range
persistent identity: range1709
display id: range1709
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation7027
display id: annotation7027
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
type: Range
persistent identity: range7027
display id: range7027
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1710
display id: annotation1710
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
type: Range
persistent identity: range1710
display id: range1710
version: 1
top level: BBa_B0032
direction: direction/forward
start: 7
end: 10
orientation: inline

mRFP1
type: ComponentDefinition
persistent identity: BBa E1010
display id: BBa_E1010
version: 1
was generated by: iGEM to SBOL conversion
description: **highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)
created: 2004-07-27T11:00:00Z
modified: 2015-08-31T04:07:26Z
mutable provenance: Campbell et al., PNAS v99 p7877 URL
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: monomeric RFP: Red Fluorescent Protein. Excitation peak: 584 nm Emission peak: 607 nm
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 52
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: TAATAA double stop codon added (DE). Four silent mutations made to remove three EcoRI sites and one PstI site: A28G, A76G, A349G, G337A.
star: true
creator: Drew Endy
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: mrfp1
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_E1010_sequence/1

mrfp1
type: SequenceAnnotation
persistent identity: annotation1014044
display id: annotation1014044
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
type: Range
persistent identity: range1014044
display id: range1014044
version: 1
top level: mRFP1
direction: direction/forward
start: 1
end: 675
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214014
display id: annotation2214014
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
type: Range
persistent identity: range2214014
display id: range2214014
version: 1
top level: mRFP1
direction: direction/forward
start: 682
end: 706
orientation: inline

BBa_B0030
type: ComponentDefinition
persistent identity: BBa B0030
display id: BBa_B0030
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.1 (strong) -- modified from R. Weiss
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _44_46_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS-1\Strong
sequence annotation: BBa_B0030
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0030_sequence/1

RBS-1\Strong
type: SequenceAnnotation
persistent identity: annotation1701
display id: annotation1701
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
type: Range
persistent identity: range1701
display id: range1701
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation7025
display id: annotation7025
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
type: Range
persistent identity: range7025
display id: range7025
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1702
display id: annotation1702
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
type: Range
persistent identity: range1702
display id: range1702
version: 1
top level: BBa_B0030
direction: direction/forward
start: 8
end: 12
orientation: inline

GFP
type: ComponentDefinition
persistent identity: BBa E0040
display id: BBa_E0040
version: 1
was generated by: iGEM to SBOL conversion
description: green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
created: 2004-09-29T11:00:00Z
modified: 2016-01-26T02:09:38Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: GFP (mut3b) [note that this part does not have a barcode]
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 4206
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jcbraff
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: GFP protein
sequence: https://synbiohub.org/public/igem/BBa_E0040_sequence/1

GFP protein
type: SequenceAnnotation
persistent identity: annotation1934520
display id: annotation1934520
version: 1
top level: GFP
location: https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
type: Range
persistent identity: range1934520
display id: range1934520
version: 1
top level: GFP
direction: direction/forward
start: 1
end: 720
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

https://synbiohub.org/public/igem/BBa_K1725103_sequence/1
type: Sequence
persistent identity: BBa K1725103 sequence
display id: BBa_K1725103_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gattcgttaccaattgacagctagctcagtcctaggtatatacatacatgcttgtttgtttgtaaactactagagattaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagaggattcgttaccaattgacatgatacgaaacgtaccgtatcgttaaggttactagagtcacacaggaaagtactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1725102_sequence/1
type: Sequence
persistent identity: BBa K1725102 sequence
display id: BBa_K1725102_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gattcgttaccaattgacagctagctcagtcctaggtatatacatacatgcttgtttgtttgtaaactactagagattaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1725002_sequence/1
type: Sequence
persistent identity: BBa K1725002 sequence
display id: BBa_K1725002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gattcgttaccaattgacatgatacgaaacgtaccgtatcgttaaggttactagagtcacacaggaaagtactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1725000_sequence/1
type: Sequence
persistent identity: BBa K1725000 sequence
display id: BBa_K1725000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gattcgttaccaattgacatgatacgaaacgtaccgtatcgttaaggt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1725020_sequence/1
type: Sequence
persistent identity: BBa K1725020 sequence
display id: BBa_K1725020_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gattcgttaccaattgacagctagctcagtcctaggtatatacatacatgcttgtttgtttgtaaac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E5501_sequence/1
type: Sequence
persistent identity: BBa E5501 sequence
display id: BBa_E5501_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaagtactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K516030_sequence/1
type: Sequence
persistent identity: BBa K516030 sequence
display id: BBa_K516030_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0032_sequence/1
type: Sequence
persistent identity: BBa B0032 sequence
display id: BBa_B0032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E1010_sequence/1
type: Sequence
persistent identity: BBa E1010 sequence
display id: BBa_E1010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0030_sequence/1
type: Sequence
persistent identity: BBa B0030 sequence
display id: BBa_B0030_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0040_sequence/1
type: Sequence
persistent identity: BBa E0040 sequence
display id: BBa_E0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: myers
owned by: james
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z