Yhbj fused to T25 domain of CyaA with cAMP-induced RFP reporter
The RNase adapter protein YhbJ fused to the T25-domain of the adenylate cyclase, CyaA, from Bordetella pertussis. This construct can be used in the two-hybrid screening of protein-protein interactions between YhbJ and another x protein fused to the T18 domain. If a random set of peptide aptamers is conjugated to the T18-domain, one can screen for specific peptide aptamers against YhbJ.
mRFP generator controlled by PcstA (glucose-repressible promoter)
Escherichia coli str. K-12 substr. MG1655
Lambda cI is controlled by promoter PcstA,which can be activated by CRP-cAMP complex.therefore protein lambda cI will be expressed under low glucose concentration.This generator contains a RFP after the promoter as a reporter.
Built by Josh as an intermediate in screening plasmid construction.
This is the promoter for the ''Eschaerichia coli'' JM109 ''cstA'' gene. It includes the CRP-binding site and the RNA polymerase-binding site. Low glucose concentration results in increased activity by adenylate cyclase. cAMP binds to the cAMP receptor protein, which, in its bound form, is able to associate with the promoter and promote transcription of the downstream gene. (''cstA'' encodes the carbon starvation protein.)
T25 domain of cyaA from Bordetella pertussis (IPTG inducible)
The T25 domain of CyaA to be used in the bacterial two-hybrid system. The expression of the gene is under control of a lac operator and is induced by IPTG. When protein-coding genes is suffixed to the T25 and T18 domains of the bacterial two-hybrid system, the interaction of these two proteins can be examined. If the protein associates, T25 and T18 associates too, and convert ATP to cAMP.
8 aa linker without BamHI restriction site and TEV recognition site
This part is similar to the 10 aa linker with BamHI restriction site and TEV recognition site, but without the initial BamHI restriction site.
RBS (Elowitz 1999) -- defines RBS efficiency
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Transcription terminator for the E.coli RNA polymerase.
Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
Transcriptional terminator consisting of a 64 bp stem-loop.
**highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)
Campbell et al., PNAS v99 p7877 URL
monomeric RFP: Red Fluorescent Protein. Excitation peak: 584 nm Emission peak: 607 nm
TAATAA double stop codon added (DE). Four silent mutations made to remove three EcoRI sites and one PstI site: A28G, A76G, A349G, G337A.
T25 domain of CyaA from Bordetella pertussis
This part codes for the T25 domain of the adenylate cyclase, CyaA, from Bordetella pertussis. When associated with the T18 domain of CyaA, the two domains together become active and catalyzes the conversion of ATP to cAMP
Conversion of the iGEM parts registry to SBOL2.1