BBa_K1407015
type: ComponentDefinition
persistent identity: BBa K1407015
display id: BBa_K1407015
version: 1
was generated by: iGEM to SBOL conversion
description: LasR+LasR/PAI1 Inducible promoter+GFP+RhlR+RhlR/PAI2 Inducible promoter+RFP
created: 2014-10-09T11:00:00Z
modified: 2015-05-08T01:10:18Z
mutable provenance: Existing parts on the registry.
owned by: james
owned by: myers
mutable description: It's composed of seven sections:constitutive production of LasR,LasR/PAI1 Inducible promoter with RBS(B0034)and GFP,terminator,constitutive promoter,RhlR protein generator ,RhlR/PAI2 inducible promoter with RBS (B0030) and RFP,Terminator.When PAI1 exsits,the part could be inducibled and expresses GFP protein;When PAI2 exsits,the part could be inducibled and expresses RFP protein.
discontinued: false
dominant: false
group u list: _1785_
m user id: 0
owner id: 23052
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: None.
star: false
creator: Ye Chen
type: DnaRegion
role: partType/Composite
role: SO:0000804
sequence annotation: BBa_J23100
sequence annotation: BBa_K553003
sequence annotation: BBa_K1407008
sequence annotation: BBa_B0034
sequence annotation: conserved
sequence annotation: BBa_C0179
sequence annotation: BBa_B0010
sequence annotation: BBa_B0015
sequence annotation: stem_loop
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence annotation: BBa_R0079
sequence annotation: BBa_K575009
sequence annotation: OP2
sequence annotation: OP1
sequence annotation: -35
sequence annotation: -10
sequence annotation: GFP protein
sequence annotation: BBa_E0040
sequence annotation: BBa_K1407011
sequence annotation: BBa_J23119
sequence annotation: BBa_I0466
sequence annotation: rhlR
sequence annotation: BBa_C0071
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence annotation: BBa_K575014
sequence annotation: RhlR
sequence annotation: BBa_R0071
sequence annotation: start
sequence annotation: BBa_B0030

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2413211
display id: annotation2413211
version: 1
top level: BBa_K1407015
location: https://synbiohub.org/public/igem/BBa_K1407015/annotation2413211/range2413211/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K1407015/annotation2413211/range2413211/1
type: Range
persistent identity: range2413211
display id: range2413211
version: 1
top level: BBa_K1407015
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_K553003
type: SequenceAnnotation
persistent identity: annotation2413212
display id: annotation2413212
version: 1
top level: BBa_K1407015
location: https://synbiohub.org/public/igem/BBa_K1407015/annotation2413212/range2413212/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K1407015/annotation2413212/range2413212/1
type: Range
persistent identity: range2413212
display id: range2413212
version: 1
top level: BBa_K1407015
direction: direction/reverse
start: 1
end: 921
orientation: inline

BBa_K1407008
type: SequenceAnnotation
persistent identity: annotation2413240
display id: annotation2413240
version: 1
top level: BBa_K1407015
location: https://synbiohub.org/public/igem/BBa_K1407015/annotation2413240/range2413240/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_K1407015/annotation2413240/range2413240/1
type: Range
persistent identity: range2413240
display id: range2413240
version: 1
top level: BBa_K1407015
direction: direction/reverse
start: 1
end: 1969
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2413213
display id: annotation2413213
version: 1
top level: BBa_K1407015
location: https://synbiohub.org/public/igem/BBa_K1407015/annotation2413213/range2413213/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K1407015/annotation2413213/range2413213/1
type: Range
persistent identity: range2413213
display id: range2413213
version: 1
top level: BBa_K1407015
direction: direction/reverse
start: 44
end: 55
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation2413214
display id: annotation2413214
version: 1
top level: BBa_K1407015
location: https://synbiohub.org/public/igem/BBa_K1407015/annotation2413214/range2413214/1
role: feature/conserved
role: SO:0000330

https://synbiohub.org/public/igem/BBa_K1407015/annotation2413214/range2413214/1
type: Range
persistent identity: range2413214
display id: range2413214
version: 1
top level: BBa_K1407015
direction: direction/forward
start: 48
end: 51
orientation: inline

BBa_C0179
type: SequenceAnnotation
persistent identity: annotation2413215
display id: annotation2413215
version: 1
top level: BBa_K1407015
location: https://synbiohub.org/public/igem/BBa_K1407015/annotation2413215/range2413215/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K1407015/annotation2413215/range2413215/1
type: Range
persistent identity: range2413215
display id: range2413215
version: 1
top level: BBa_K1407015
direction: direction/reverse
start: 62
end: 784
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2413217
display id: annotation2413217
version: 1
top level: BBa_K1407015
location: https://synbiohub.org/public/igem/BBa_K1407015/annotation2413217/range2413217/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_K1407015/annotation2413217/range2413217/1
type: Range
persistent identity: range2413217
display id: range2413217
version: 1
top level: BBa_K1407015
direction: direction/forward
start: 793
end: 872
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2413216
display id: annotation2413216
version: 1
top level: BBa_K1407015
location: https://synbiohub.org/public/igem/BBa_K1407015/annotation2413216/range2413216/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K1407015/annotation2413216/range2413216/1
type: Range
persistent identity: range2413216
display id: range2413216
version: 1
top level: BBa_K1407015
direction: direction/reverse
start: 793
end: 921
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation2413218
display id: annotation2413218
version: 1
top level: BBa_K1407015
location: https://synbiohub.org/public/igem/BBa_K1407015/annotation2413218/range2413218/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_K1407015/annotation2413218/range2413218/1
type: Range
persistent identity: range2413218
display id: range2413218
version: 1
top level: BBa_K1407015
direction: direction/forward
start: 804
end: 847
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2413219
display id: annotation2413219
version: 1
top level: BBa_K1407015
location: https://synbiohub.org/public/igem/BBa_K1407015/annotation2413219/range2413219/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_K1407015/annotation2413219/range2413219/1
type: Range
persistent identity: range2413219
display id: range2413219
version: 1
top level: BBa_K1407015
direction: direction/forward
start: 881
end: 921
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation2413220
display id: annotation2413220
version: 1
top level: BBa_K1407015
location: https://synbiohub.org/public/igem/BBa_K1407015/annotation2413220/range2413220/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_K1407015/annotation2413220/range2413220/1
type: Range
persistent identity: range2413220
display id: range2413220
version: 1
top level: BBa_K1407015
direction: direction/forward
start: 888
end: 907
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation2413221
display id: annotation2413221
version: 1
top level: BBa_K1407015
location: https://synbiohub.org/public/igem/BBa_K1407015/annotation2413221/range2413221/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_K1407015/annotation2413221/range2413221/1
type: Range
persistent identity: range2413221
display id: range2413221
version: 1
top level: BBa_K1407015
direction: direction/forward
start: 908
end: 921
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2413222
display id: annotation2413222
version: 1
top level: BBa_K1407015
location: https://synbiohub.org/public/igem/BBa_K1407015/annotation2413222/range2413222/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K1407015/annotation2413222/range2413222/1
type: Range
persistent identity: range2413222
display id: range2413222
version: 1
top level: BBa_K1407015
direction: direction/forward
start: 914
end: 914
orientation: inline

BBa_R0079
type: SequenceAnnotation
persistent identity: annotation2413223
display id: annotation2413223
version: 1
top level: BBa_K1407015
location: https://synbiohub.org/public/igem/BBa_K1407015/annotation2413223/range2413223/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_K1407015/annotation2413223/range2413223/1
type: Range
persistent identity: range2413223
display id: range2413223
version: 1
top level: BBa_K1407015
direction: direction/reverse
start: 930
end: 1086
orientation: inline

BBa_K575009
type: SequenceAnnotation
persistent identity: annotation2413224
display id: annotation2413224
version: 1
top level: BBa_K1407015
location: https://synbiohub.org/public/igem/BBa_K1407015/annotation2413224/range2413224/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_K1407015/annotation2413224/range2413224/1
type: Range
persistent identity: range2413224
display id: range2413224
version: 1
top level: BBa_K1407015
direction: direction/reverse
start: 930
end: 1832
orientation: inline

OP2
type: SequenceAnnotation
persistent identity: annotation2413225
display id: annotation2413225
version: 1
top level: BBa_K1407015
location: https://synbiohub.org/public/igem/BBa_K1407015/annotation2413225/range2413225/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_K1407015/annotation2413225/range2413225/1
type: Range
persistent identity: range2413225
display id: range2413225
version: 1
top level: BBa_K1407015
direction: direction/forward
start: 975
end: 992
orientation: inline

OP1
type: SequenceAnnotation
persistent identity: annotation2413226
display id: annotation2413226
version: 1
top level: BBa_K1407015
location: https://synbiohub.org/public/igem/BBa_K1407015/annotation2413226/range2413226/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_K1407015/annotation2413226/range2413226/1
type: Range
persistent identity: range2413226
display id: range2413226
version: 1
top level: BBa_K1407015
direction: direction/forward
start: 1035
end: 1054
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2413227
display id: annotation2413227
version: 1
top level: BBa_K1407015
location: https://synbiohub.org/public/igem/BBa_K1407015/annotation2413227/range2413227/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_K1407015/annotation2413227/range2413227/1
type: Range
persistent identity: range2413227
display id: range2413227
version: 1
top level: BBa_K1407015
direction: direction/forward
start: 1046
end: 1051
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2413228
display id: annotation2413228
version: 1
top level: BBa_K1407015
location: https://synbiohub.org/public/igem/BBa_K1407015/annotation2413228/range2413228/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_K1407015/annotation2413228/range2413228/1
type: Range
persistent identity: range2413228
display id: range2413228
version: 1
top level: BBa_K1407015
direction: direction/forward
start: 1069
end: 1074
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2413229
display id: annotation2413229
version: 1
top level: BBa_K1407015
location: https://synbiohub.org/public/igem/BBa_K1407015/annotation2413229/range2413229/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_K1407015/annotation2413229/range2413229/1
type: Range
persistent identity: range2413229
display id: range2413229
version: 1
top level: BBa_K1407015
direction: direction/reverse
start: 1095
end: 1106
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation2413230
display id: annotation2413230
version: 1
top level: BBa_K1407015
location: https://synbiohub.org/public/igem/BBa_K1407015/annotation2413230/range2413230/1
role: feature/conserved
role: SO:0000330

https://synbiohub.org/public/igem/BBa_K1407015/annotation2413230/range2413230/1
type: Range
persistent identity: range2413230
display id: range2413230
version: 1
top level: BBa_K1407015
direction: direction/forward
start: 1099
end: 1102
orientation: inline

GFP protein
type: SequenceAnnotation
persistent identity: annotation2413231
display id: annotation2413231
version: 1
top level: BBa_K1407015
location: https://synbiohub.org/public/igem/BBa_K1407015/annotation2413231/range2413231/1
role: feature/protein
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K1407015/annotation2413231/range2413231/1
type: Range
persistent identity: range2413231
display id: range2413231
version: 1
top level: BBa_K1407015
direction: direction/forward
start: 1113
end: 1832
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2413232
display id: annotation2413232
version: 1
top level: BBa_K1407015
location: https://synbiohub.org/public/igem/BBa_K1407015/annotation2413232/range2413232/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_K1407015/annotation2413232/range2413232/1
type: Range
persistent identity: range2413232
display id: range2413232
version: 1
top level: BBa_K1407015
direction: direction/reverse
start: 1113
end: 1832
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2413233
display id: annotation2413233
version: 1
top level: BBa_K1407015
location: https://synbiohub.org/public/igem/BBa_K1407015/annotation2413233/range2413233/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_K1407015/annotation2413233/range2413233/1
type: Range
persistent identity: range2413233
display id: range2413233
version: 1
top level: BBa_K1407015
direction: direction/forward
start: 1841
end: 1920
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2413234
display id: annotation2413234
version: 1
top level: BBa_K1407015
location: https://synbiohub.org/public/igem/BBa_K1407015/annotation2413234/range2413234/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K1407015/annotation2413234/range2413234/1
type: Range
persistent identity: range2413234
display id: range2413234
version: 1
top level: BBa_K1407015
direction: direction/reverse
start: 1841
end: 1969
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation2413235
display id: annotation2413235
version: 1
top level: BBa_K1407015
location: https://synbiohub.org/public/igem/BBa_K1407015/annotation2413235/range2413235/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_K1407015/annotation2413235/range2413235/1
type: Range
persistent identity: range2413235
display id: range2413235
version: 1
top level: BBa_K1407015
direction: direction/forward
start: 1852
end: 1895
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2413236
display id: annotation2413236
version: 1
top level: BBa_K1407015
location: https://synbiohub.org/public/igem/BBa_K1407015/annotation2413236/range2413236/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K1407015/annotation2413236/range2413236/1
type: Range
persistent identity: range2413236
display id: range2413236
version: 1
top level: BBa_K1407015
direction: direction/forward
start: 1929
end: 1969
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation2413237
display id: annotation2413237
version: 1
top level: BBa_K1407015
location: https://synbiohub.org/public/igem/BBa_K1407015/annotation2413237/range2413237/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_K1407015/annotation2413237/range2413237/1
type: Range
persistent identity: range2413237
display id: range2413237
version: 1
top level: BBa_K1407015
direction: direction/forward
start: 1936
end: 1955
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation2413238
display id: annotation2413238
version: 1
top level: BBa_K1407015
location: https://synbiohub.org/public/igem/BBa_K1407015/annotation2413238/range2413238/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_K1407015/annotation2413238/range2413238/1
type: Range
persistent identity: range2413238
display id: range2413238
version: 1
top level: BBa_K1407015
direction: direction/forward
start: 1956
end: 1969
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2413239
display id: annotation2413239
version: 1
top level: BBa_K1407015
location: https://synbiohub.org/public/igem/BBa_K1407015/annotation2413239/range2413239/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K1407015/annotation2413239/range2413239/1
type: Range
persistent identity: range2413239
display id: range2413239
version: 1
top level: BBa_K1407015
direction: direction/forward
start: 1962
end: 1962
orientation: inline

BBa_K1407011
type: SequenceAnnotation
persistent identity: annotation2413274
display id: annotation2413274
version: 1
top level: BBa_K1407015
location: https://synbiohub.org/public/igem/BBa_K1407015/annotation2413274/range2413274/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K1407015/annotation2413274/range2413274/1
type: Range
persistent identity: range2413274
display id: range2413274
version: 1
top level: BBa_K1407015
direction: direction/reverse
start: 1978
end: 3895
orientation: inline

BBa_J23119
type: SequenceAnnotation
persistent identity: annotation2413241
display id: annotation2413241
version: 1
top level: BBa_K1407015
location: https://synbiohub.org/public/igem/BBa_K1407015/annotation2413241/range2413241/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K1407015/annotation2413241/range2413241/1
type: Range
persistent identity: range2413241
display id: range2413241
version: 1
top level: BBa_K1407015
direction: direction/reverse
start: 1978
end: 2012
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2413243
display id: annotation2413243
version: 1
top level: BBa_K1407015
location: https://synbiohub.org/public/igem/BBa_K1407015/annotation2413243/range2413243/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_K1407015/annotation2413243/range2413243/1
type: Range
persistent identity: range2413243
display id: range2413243
version: 1
top level: BBa_K1407015
direction: direction/forward
start: 2021
end: 2032
orientation: inline

BBa_I0466
type: SequenceAnnotation
persistent identity: annotation2413242
display id: annotation2413242
version: 1
top level: BBa_K1407015
location: https://synbiohub.org/public/igem/BBa_K1407015/annotation2413242/range2413242/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K1407015/annotation2413242/range2413242/1
type: Range
persistent identity: range2413242
display id: range2413242
version: 1
top level: BBa_K1407015
direction: direction/reverse
start: 2021
end: 2962
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation2413244
display id: annotation2413244
version: 1
top level: BBa_K1407015
location: https://synbiohub.org/public/igem/BBa_K1407015/annotation2413244/range2413244/1
role: feature/conserved
role: SO:0000330

https://synbiohub.org/public/igem/BBa_K1407015/annotation2413244/range2413244/1
type: Range
persistent identity: range2413244
display id: range2413244
version: 1
top level: BBa_K1407015
direction: direction/forward
start: 2025
end: 2028
orientation: inline

rhlR
type: SequenceAnnotation
persistent identity: annotation2413245
display id: annotation2413245
version: 1
top level: BBa_K1407015
location: https://synbiohub.org/public/igem/BBa_K1407015/annotation2413245/range2413245/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K1407015/annotation2413245/range2413245/1
type: Range
persistent identity: range2413245
display id: range2413245
version: 1
top level: BBa_K1407015
direction: direction/forward
start: 2039
end: 2761
orientation: inline

BBa_C0071
type: SequenceAnnotation
persistent identity: annotation2413246
display id: annotation2413246
version: 1
top level: BBa_K1407015
location: https://synbiohub.org/public/igem/BBa_K1407015/annotation2413246/range2413246/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K1407015/annotation2413246/range2413246/1
type: Range
persistent identity: range2413246
display id: range2413246
version: 1
top level: BBa_K1407015
direction: direction/forward
start: 2039
end: 2800
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation2413247
display id: annotation2413247
version: 1
top level: BBa_K1407015
location: https://synbiohub.org/public/igem/BBa_K1407015/annotation2413247/range2413247/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_K1407015/annotation2413247/range2413247/1
type: Range
persistent identity: range2413247
display id: range2413247
version: 1
top level: BBa_K1407015
direction: direction/forward
start: 2762
end: 2794
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2413248
display id: annotation2413248
version: 1
top level: BBa_K1407015
location: https://synbiohub.org/public/igem/BBa_K1407015/annotation2413248/range2413248/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_K1407015/annotation2413248/range2413248/1
type: Range
persistent identity: range2413248
display id: range2413248
version: 1
top level: BBa_K1407015
direction: direction/forward
start: 2801
end: 2825
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2413249
display id: annotation2413249
version: 1
top level: BBa_K1407015
location: https://synbiohub.org/public/igem/BBa_K1407015/annotation2413249/range2413249/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K1407015/annotation2413249/range2413249/1
type: Range
persistent identity: range2413249
display id: range2413249
version: 1
top level: BBa_K1407015
direction: direction/forward
start: 2834
end: 2913
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation2413250
display id: annotation2413250
version: 1
top level: BBa_K1407015
location: https://synbiohub.org/public/igem/BBa_K1407015/annotation2413250/range2413250/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_K1407015/annotation2413250/range2413250/1
type: Range
persistent identity: range2413250
display id: range2413250
version: 1
top level: BBa_K1407015
direction: direction/forward
start: 2845
end: 2888
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2413251
display id: annotation2413251
version: 1
top level: BBa_K1407015
location: https://synbiohub.org/public/igem/BBa_K1407015/annotation2413251/range2413251/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_K1407015/annotation2413251/range2413251/1
type: Range
persistent identity: range2413251
display id: range2413251
version: 1
top level: BBa_K1407015
direction: direction/forward
start: 2922
end: 2962
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation2413252
display id: annotation2413252
version: 1
top level: BBa_K1407015
location: https://synbiohub.org/public/igem/BBa_K1407015/annotation2413252/range2413252/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_K1407015/annotation2413252/range2413252/1
type: Range
persistent identity: range2413252
display id: range2413252
version: 1
top level: BBa_K1407015
direction: direction/forward
start: 2929
end: 2948
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation2413253
display id: annotation2413253
version: 1
top level: BBa_K1407015
location: https://synbiohub.org/public/igem/BBa_K1407015/annotation2413253/range2413253/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_K1407015/annotation2413253/range2413253/1
type: Range
persistent identity: range2413253
display id: range2413253
version: 1
top level: BBa_K1407015
direction: direction/forward
start: 2949
end: 2962
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2413254
display id: annotation2413254
version: 1
top level: BBa_K1407015
location: https://synbiohub.org/public/igem/BBa_K1407015/annotation2413254/range2413254/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_K1407015/annotation2413254/range2413254/1
type: Range
persistent identity: range2413254
display id: range2413254
version: 1
top level: BBa_K1407015
direction: direction/forward
start: 2955
end: 2955
orientation: inline

BBa_K575014
type: SequenceAnnotation
persistent identity: annotation2413257
display id: annotation2413257
version: 1
top level: BBa_K1407015
location: https://synbiohub.org/public/igem/BBa_K1407015/annotation2413257/range2413257/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K1407015/annotation2413257/range2413257/1
type: Range
persistent identity: range2413257
display id: range2413257
version: 1
top level: BBa_K1407015
direction: direction/reverse
start: 2971
end: 3758
orientation: inline

RhlR
type: SequenceAnnotation
persistent identity: annotation2413255
display id: annotation2413255
version: 1
top level: BBa_K1407015
location: https://synbiohub.org/public/igem/BBa_K1407015/annotation2413255/range2413255/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_K1407015/annotation2413255/range2413255/1
type: Range
persistent identity: range2413255
display id: range2413255
version: 1
top level: BBa_K1407015
direction: direction/forward
start: 2971
end: 2990
orientation: inline

BBa_R0071
type: SequenceAnnotation
persistent identity: annotation2413256
display id: annotation2413256
version: 1
top level: BBa_K1407015
location: https://synbiohub.org/public/igem/BBa_K1407015/annotation2413256/range2413256/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_K1407015/annotation2413256/range2413256/1
type: Range
persistent identity: range2413256
display id: range2413256
version: 1
top level: BBa_K1407015
direction: direction/reverse
start: 2971
end: 3023
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2413258
display id: annotation2413258
version: 1
top level: BBa_K1407015
location: https://synbiohub.org/public/igem/BBa_K1407015/annotation2413258/range2413258/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K1407015/annotation2413258/range2413258/1
type: Range
persistent identity: range2413258
display id: range2413258
version: 1
top level: BBa_K1407015
direction: direction/forward
start: 2989
end: 2994
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2413259
display id: annotation2413259
version: 1
top level: BBa_K1407015
location: https://synbiohub.org/public/igem/BBa_K1407015/annotation2413259/range2413259/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K1407015/annotation2413259/range2413259/1
type: Range
persistent identity: range2413259
display id: range2413259
version: 1
top level: BBa_K1407015
direction: direction/forward
start: 3012
end: 3017
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2413260
display id: annotation2413260
version: 1
top level: BBa_K1407015
location: https://synbiohub.org/public/igem/BBa_K1407015/annotation2413260/range2413260/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_K1407015/annotation2413260/range2413260/1
type: Range
persistent identity: range2413260
display id: range2413260
version: 1
top level: BBa_K1407015
direction: direction/forward
start: 3023
end: 3023
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2413261
display id: annotation2413261
version: 1
top level: BBa_K1407015
location: https://synbiohub.org/public/igem/BBa_K1407015/annotation2413261/range2413261/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K1407015/annotation2413261/range2413261/1
type: Range
persistent identity: range2413261
display id: range2413261
version: 1
top level: BBa_K1407015
direction: direction/forward
start: 3032
end: 3046
orientation: inline

RBS-1\Strong
type: SequenceAnnotation
persistent identity: annotation2413262
display id: annotation2413262
version: 1
top level: BBa_K1407015
location: https://synbiohub.org/public/igem/BBa_K1407015/annotation2413262/range2413262/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_K1407015/annotation2413262/range2413262/1
type: Range
persistent identity: range2413262
display id: range2413262
version: 1
top level: BBa_K1407015
direction: direction/forward
start: 3032
end: 3046
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2413263
display id: annotation2413263
version: 1
top level: BBa_K1407015
location: https://synbiohub.org/public/igem/BBa_K1407015/annotation2413263/range2413263/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_K1407015/annotation2413263/range2413263/1
type: Range
persistent identity: range2413263
display id: range2413263
version: 1
top level: BBa_K1407015
direction: direction/forward
start: 3039
end: 3043
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2413265
display id: annotation2413265
version: 1
top level: BBa_K1407015
location: https://synbiohub.org/public/igem/BBa_K1407015/annotation2413265/range2413265/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_K1407015/annotation2413265/range2413265/1
type: Range
persistent identity: range2413265
display id: range2413265
version: 1
top level: BBa_K1407015
direction: direction/reverse
start: 3053
end: 3758
orientation: inline

mrfp1
type: SequenceAnnotation
persistent identity: annotation2413264
display id: annotation2413264
version: 1
top level: BBa_K1407015
location: https://synbiohub.org/public/igem/BBa_K1407015/annotation2413264/range2413264/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K1407015/annotation2413264/range2413264/1
type: Range
persistent identity: range2413264
display id: range2413264
version: 1
top level: BBa_K1407015
direction: direction/forward
start: 3053
end: 3727
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2413266
display id: annotation2413266
version: 1
top level: BBa_K1407015
location: https://synbiohub.org/public/igem/BBa_K1407015/annotation2413266/range2413266/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_K1407015/annotation2413266/range2413266/1
type: Range
persistent identity: range2413266
display id: range2413266
version: 1
top level: BBa_K1407015
direction: direction/forward
start: 3734
end: 3758
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2413267
display id: annotation2413267
version: 1
top level: BBa_K1407015
location: https://synbiohub.org/public/igem/BBa_K1407015/annotation2413267/range2413267/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K1407015/annotation2413267/range2413267/1
type: Range
persistent identity: range2413267
display id: range2413267
version: 1
top level: BBa_K1407015
direction: direction/forward
start: 3767
end: 3846
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2413268
display id: annotation2413268
version: 1
top level: BBa_K1407015
location: https://synbiohub.org/public/igem/BBa_K1407015/annotation2413268/range2413268/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_K1407015/annotation2413268/range2413268/1
type: Range
persistent identity: range2413268
display id: range2413268
version: 1
top level: BBa_K1407015
direction: direction/reverse
start: 3767
end: 3895
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation2413269
display id: annotation2413269
version: 1
top level: BBa_K1407015
location: https://synbiohub.org/public/igem/BBa_K1407015/annotation2413269/range2413269/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_K1407015/annotation2413269/range2413269/1
type: Range
persistent identity: range2413269
display id: range2413269
version: 1
top level: BBa_K1407015
direction: direction/forward
start: 3778
end: 3821
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2413270
display id: annotation2413270
version: 1
top level: BBa_K1407015
location: https://synbiohub.org/public/igem/BBa_K1407015/annotation2413270/range2413270/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K1407015/annotation2413270/range2413270/1
type: Range
persistent identity: range2413270
display id: range2413270
version: 1
top level: BBa_K1407015
direction: direction/forward
start: 3855
end: 3895
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation2413271
display id: annotation2413271
version: 1
top level: BBa_K1407015
location: https://synbiohub.org/public/igem/BBa_K1407015/annotation2413271/range2413271/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_K1407015/annotation2413271/range2413271/1
type: Range
persistent identity: range2413271
display id: range2413271
version: 1
top level: BBa_K1407015
direction: direction/forward
start: 3862
end: 3881
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation2413272
display id: annotation2413272
version: 1
top level: BBa_K1407015
location: https://synbiohub.org/public/igem/BBa_K1407015/annotation2413272/range2413272/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_K1407015/annotation2413272/range2413272/1
type: Range
persistent identity: range2413272
display id: range2413272
version: 1
top level: BBa_K1407015
direction: direction/forward
start: 3882
end: 3895
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2413273
display id: annotation2413273
version: 1
top level: BBa_K1407015
location: https://synbiohub.org/public/igem/BBa_K1407015/annotation2413273/range2413273/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_K1407015/annotation2413273/range2413273/1
type: Range
persistent identity: range2413273
display id: range2413273
version: 1
top level: BBa_K1407015
direction: direction/forward
start: 3888
end: 3888
orientation: inline

https://synbiohub.org/public/igem/BBa_K1407015_sequence/1
type: Sequence
persistent identity: BBa K1407015 sequence
display id: BBa_K1407015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtacagtgctagctactagagaaagaggagaaatactagatggccttggttgacggttttcttgagctggaacgctcaagtggaaaattggagtggagcgccatcctccagaagatggcgagcgaccttggattctcgaagatcctgttcggcctgttgcctaaggacagccaggactacgagaacgccttcatcgtcggcaactacccggccgcctggcgcgagcattacgaccgggctggctacgcgcgggtcgacccgacggtcagtcactgtacccagagcgtactgccgattttctgggaaccgtccatctaccagacgcgaaagcagcacgagttcttcgaggaagcctcggccgccggcctggtgtatgggctgaccatgccgctgcatggtgctcgcggcgaactcggcgcgctgagcctcagcgtggaagcggaaaaccgggccgaggccaaccgtttcatagagtcggtcctgccgaccctgtggatgctcaaggactacgcactgcaaagcggtgccggactggccttcgaacatccggtcagcaaaccggtggttctgaccagccgggagaaggaagtgttgcagtggtgcgccatcggcaagaccagttgggagatatcggttatctgcaactgctcggaagccaatgtgaacttccatatgggaaatattcggcggaagttcggtgtgacctcccgccgcgtagcggccattatggccgttaatttgggtcttattactctctaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagaggcccctcgctgagcgcgtcccggagctgggggcaacctagctgccacctgcttttctgctagctattccagcgaaaacatacagatttccggcgaaatcaaggctacctgccagttctggcaggtttggccgcgggttctttttggtacacgaaagctactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttgacagctagctcagtcctagg…
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z