BBa_K1361000
type: ComponentDefinition
persistent identity: BBa K1361000
display id: BBa_K1361000
version: 1
was generated by: iGEM to SBOL conversion
description: Curli Fiber generator under Pbad induction with positive regulation
created: 2014-10-02T11:00:00Z
modified: 2015-05-08T01:10:03Z
mutable provenance: CsgAC,CsgB collected by PCR from genome of E coli DH5alpha respectively. Other genes are collected from Biobricks Attribution Kit.
owned by: james
owned by: myers
mutable description: We assembled this part in order to examine the concept that change of cell motility by control of CheZ chemotaxis regulator could disturb pre-expressed curli fiber structure. If the inducer occurs, in this case_acacia, CsgB protein will secrete into extracellular and act as nucleator to recruit soluble CsgA monomer into amyloid fibres, and the transcription of CheZ will be downgrade via cI-Plamda NOT gate. If induction condition disappears at a certain point, suppression of CheZ will be discharged. As the result, cells will be more moveable thus disturb or inhibit the establishment of curli structure. And in our final device, the modified CsgA that has ability to absorb nanogold, thus the dynamic detection could achieve by monitoring current change between two electrodes in the culture.
discontinued: false
dominant: false
group u list: _1737_
m user id: 0
owner id: 16915
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: The PstI loci within CsgA sequence has been replaced from CTGCAG to CTGCCG. And for nano gold absorbability, 6xLinker+2xCys(GGCAGCGGTGGCAGCGGTGGCGGT TGTTGC) and LPFFD(CTGCCGTTCTTTGAT) were added at the C-treminal of CsgA protein respectively. CsgA with7xhis tag at C-treminal were also used for Au-NTA-Ni absorbing.
star: false
creator: Shoujie Sun
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_K206000
component: BBa_K880005
component: BBa_B0015
component: BBa_K1361997
component: BBa_S0103
component: BBa_K629003
component: BBa_B0034
component: BBa_R0051
component: BBa_K1361998
sequence annotation: BBa_K206000
sequence annotation: BBa_S0103
sequence annotation: BBa_K1361997
sequence annotation: BBa_B0015
sequence annotation: BBa_K880005
sequence annotation: BBa_K1361998
sequence annotation: BBa_R0051
sequence annotation: BBa_B0034
sequence annotation: BBa_K629003
sequence: https://synbiohub.org/public/igem/BBa_K1361000_sequence/1

BBa_K206000
type: Component
persistent identity: component2392767
display id: component2392767
version: 1
top level: BBa_K1361000
definition: pBAD
access: public

BBa_K880005
type: Component
persistent identity: component2392789
display id: component2392789
version: 1
top level: BBa_K1361000
definition: BBa_K880005
access: public

BBa_B0015
type: Component
persistent identity: component2392785
display id: component2392785
version: 1
top level: BBa_K1361000
definition: BBa_B0015
access: public

BBa_K1361997
type: Component
persistent identity: component2392778
display id: component2392778
version: 1
top level: BBa_K1361000
definition: BBa_K1361997
access: public

BBa_S0103
type: Component
persistent identity: component2392774
display id: component2392774
version: 1
top level: BBa_K1361000
definition: BBa_S0103
access: public

BBa_K629003
type: Component
persistent identity: component2392810
display id: component2392810
version: 1
top level: BBa_K1361000
definition: cheZ
access: public

BBa_B0034
type: Component
persistent identity: component2392803
display id: component2392803
version: 1
top level: BBa_K1361000
definition: BBa_B0034
access: public

BBa_R0051
type: Component
persistent identity: component2392798
display id: component2392798
version: 1
top level: BBa_K1361000
definition: cI lam
access: public

BBa_K1361998
type: Component
persistent identity: component2392796
display id: component2392796
version: 1
top level: BBa_K1361000
definition: BBa_K1361998
access: public

BBa_K206000
type: SequenceAnnotation
persistent identity: annotation2392767
display id: annotation2392767
version: 1
top level: BBa_K1361000
location: https://synbiohub.org/public/igem/BBa_K1361000/annotation2392767/range2392767/1
component: BBa_K206000

https://synbiohub.org/public/igem/BBa_K1361000/annotation2392767/range2392767/1
type: Range
persistent identity: range2392767
display id: range2392767
version: 1
top level: BBa_K1361000
direction: direction/reverse
start: 1
end: 130
orientation: inline

BBa_S0103
type: SequenceAnnotation
persistent identity: annotation2392774
display id: annotation2392774
version: 1
top level: BBa_K1361000
location: https://synbiohub.org/public/igem/BBa_K1361000/annotation2392774/range2392774/1
component: BBa_S0103

https://synbiohub.org/public/igem/BBa_K1361000/annotation2392774/range2392774/1
type: Range
persistent identity: range2392774
display id: range2392774
version: 1
top level: BBa_K1361000
direction: direction/reverse
start: 139
end: 931
orientation: inline

BBa_K1361997
type: SequenceAnnotation
persistent identity: annotation2392778
display id: annotation2392778
version: 1
top level: BBa_K1361000
location: https://synbiohub.org/public/igem/BBa_K1361000/annotation2392778/range2392778/1
component: BBa_K1361997

https://synbiohub.org/public/igem/BBa_K1361000/annotation2392778/range2392778/1
type: Range
persistent identity: range2392778
display id: range2392778
version: 1
top level: BBa_K1361000
direction: direction/reverse
start: 938
end: 1393
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2392785
display id: annotation2392785
version: 1
top level: BBa_K1361000
location: https://synbiohub.org/public/igem/BBa_K1361000/annotation2392785/range2392785/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1361000/annotation2392785/range2392785/1
type: Range
persistent identity: range2392785
display id: range2392785
version: 1
top level: BBa_K1361000
direction: direction/reverse
start: 1402
end: 1530
orientation: inline

BBa_K880005
type: SequenceAnnotation
persistent identity: annotation2392789
display id: annotation2392789
version: 1
top level: BBa_K1361000
location: https://synbiohub.org/public/igem/BBa_K1361000/annotation2392789/range2392789/1
component: BBa_K880005

https://synbiohub.org/public/igem/BBa_K1361000/annotation2392789/range2392789/1
type: Range
persistent identity: range2392789
display id: range2392789
version: 1
top level: BBa_K1361000
direction: direction/reverse
start: 1539
end: 1593
orientation: inline

BBa_K1361998
type: SequenceAnnotation
persistent identity: annotation2392796
display id: annotation2392796
version: 1
top level: BBa_K1361000
location: https://synbiohub.org/public/igem/BBa_K1361000/annotation2392796/range2392796/1
component: BBa_K1361998

https://synbiohub.org/public/igem/BBa_K1361000/annotation2392796/range2392796/1
type: Range
persistent identity: range2392796
display id: range2392796
version: 1
top level: BBa_K1361000
direction: direction/reverse
start: 1600
end: 2446
orientation: inline

BBa_R0051
type: SequenceAnnotation
persistent identity: annotation2392798
display id: annotation2392798
version: 1
top level: BBa_K1361000
location: https://synbiohub.org/public/igem/BBa_K1361000/annotation2392798/range2392798/1
component: BBa_R0051

https://synbiohub.org/public/igem/BBa_K1361000/annotation2392798/range2392798/1
type: Range
persistent identity: range2392798
display id: range2392798
version: 1
top level: BBa_K1361000
direction: direction/forward
start: 2455
end: 2503
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2392803
display id: annotation2392803
version: 1
top level: BBa_K1361000
location: https://synbiohub.org/public/igem/BBa_K1361000/annotation2392803/range2392803/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1361000/annotation2392803/range2392803/1
type: Range
persistent identity: range2392803
display id: range2392803
version: 1
top level: BBa_K1361000
direction: direction/reverse
start: 2512
end: 2523
orientation: inline

BBa_K629003
type: SequenceAnnotation
persistent identity: annotation2392810
display id: annotation2392810
version: 1
top level: BBa_K1361000
location: https://synbiohub.org/public/igem/BBa_K1361000/annotation2392810/range2392810/1
component: BBa_K629003

https://synbiohub.org/public/igem/BBa_K1361000/annotation2392810/range2392810/1
type: Range
persistent identity: range2392810
display id: range2392810
version: 1
top level: BBa_K1361000
direction: direction/reverse
start: 2530
end: 3174
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

cI lam
type: ComponentDefinition
persistent identity: BBa R0051
display id: BBa_R0051
version: 1
was generated by: iGEM to SBOL conversion
description: promoter (lambda cI regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: The cI regulated promoter is based on the pR promtoer from bacteriohage lambda. The promoter has two two DNA binding sites for lambda cI repressor <bb_part>BBa_C0051</bb_part>. cI binding results in repression of transcription. The specific sequence used here is based on the cI repressible promoter used in the Elowitz repressilator (and references therein).
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: In order to address concerns about the promoter transcribing in the reverse direction, we have removed the -35 and -10 signals responsible for the promoter activity in the reverse direction. (More details needed here! DE, 2/24/03) Incompatible with host expressing cI repressor.
star: true
creator: Vinay S Mahajan, Brian Chow, Peter Carr, Voichita Marinescu and Alexander D. Wissner-Gross
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0051
sequence annotation: OR2
sequence annotation: -35
sequence annotation: OR1
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0051_sequence/1

BBa_R0051
type: SequenceAnnotation
persistent identity: annotation7067
display id: annotation7067
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R0051/annotation7067/range7067/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_R0051/annotation7067/range7067/1
type: Range
persistent identity: range7067
display id: range7067
version: 1
top level: cI lam
direction: direction/forward
start: 1
end: 49
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation2025
display id: annotation2025
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2025/range2025/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0051/annotation2025/range2025/1
type: Range
persistent identity: range2025
display id: range2025
version: 1
top level: cI lam
direction: direction/forward
start: 1
end: 17
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2023
display id: annotation2023
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2023/range2023/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0051/annotation2023/range2023/1
type: Range
persistent identity: range2023
display id: range2023
version: 1
top level: cI lam
direction: direction/forward
start: 15
end: 20
orientation: inline

OR1
type: SequenceAnnotation
persistent identity: annotation2024
display id: annotation2024
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2024/range2024/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0051/annotation2024/range2024/1
type: Range
persistent identity: range2024
display id: range2024
version: 1
top level: cI lam
direction: direction/forward
start: 25
end: 41
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2022
display id: annotation2022
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2022/range2022/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0051/annotation2022/range2022/1
type: Range
persistent identity: range2022
display id: range2022
version: 1
top level: cI lam
direction: direction/forward
start: 38
end: 43
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_S0103
type: ComponentDefinition
persistent identity: BBa S0103
display id: BBa_S0103
version: 1
was generated by: iGEM to SBOL conversion
description: B0034.C0051
created: 2003-12-04T12:00:00Z
modified: 2015-05-08T01:14:17Z
owned by: james
owned by: myers
part status: Released HQ 2013
discontinued: false
dominant: true
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: false
creator: Caitlin Conboy and Jennifer Braff
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_B0034
component: BBa_C0051
sequence annotation: BBa_B0034
sequence annotation: BBa_C0051
sequence: https://synbiohub.org/public/igem/BBa_S0103_sequence/1

BBa_B0034
type: Component
persistent identity: component939748
display id: component939748
version: 1
top level: BBa_S0103
definition: BBa_B0034
access: public

BBa_C0051
type: Component
persistent identity: component939758
display id: component939758
version: 1
top level: BBa_S0103
definition: cI lam
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation939748
display id: annotation939748
version: 1
top level: BBa_S0103
location: https://synbiohub.org/public/igem/BBa_S0103/annotation939748/range939748/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_S0103/annotation939748/range939748/1
type: Range
persistent identity: range939748
display id: range939748
version: 1
top level: BBa_S0103
direction: direction/forward
start: 1
end: 12
orientation: inline

BBa_C0051
type: SequenceAnnotation
persistent identity: annotation939758
display id: annotation939758
version: 1
top level: BBa_S0103
location: https://synbiohub.org/public/igem/BBa_S0103/annotation939758/range939758/1
component: BBa_C0051

https://synbiohub.org/public/igem/BBa_S0103/annotation939758/range939758/1
type: Range
persistent identity: range939758
display id: range939758
version: 1
top level: BBa_S0103
direction: direction/forward
start: 19
end: 768
orientation: inline

cheZ
type: ComponentDefinition
persistent identity: BBa K629003
display id: BBa_K629003
version: 1
was generated by: iGEM to SBOL conversion
description: cheZ, chemotaxis regulator, protein phosphatase for CheY
created: 2011-10-02T11:00:00Z
modified: 2015-05-08T01:12:56Z
mutable provenance: genome of E. Coli BL21 plys strain
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Polar localization is dependent on CheA-short. CheZ is a cytosolic phosphatase which functions in the chemotaxis signal transduction complex. Plays an important role in bacterial chemotaxis signal transduction pathway by accelerating the dephosphorylation of phosphorylated CheY (CheY-P).
discontinued: false
dominant: false
experience: experience/Works
group u list: _801_
m user id: 0
owner id: 9022
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: how to control the proper expression of cheZ
star: false
creator: Zilong WANG, Yi ZHENG
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: CheZ
sequence annotation: fwd primer
sequence annotation: start
sequence annotation: rev primer
sequence annotation: TGA to TAA
sequence annotation: terminator
sequence: https://synbiohub.org/public/igem/BBa_K629003_sequence/1

CheZ
type: SequenceAnnotation
persistent identity: annotation2154425
display id: annotation2154425
version: 1
top level: cheZ
location: https://synbiohub.org/public/igem/BBa_K629003/annotation2154425/range2154425/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K629003/annotation2154425/range2154425/1
type: Range
persistent identity: range2154425
display id: range2154425
version: 1
top level: cheZ
direction: direction/forward
start: 1
end: 645
orientation: inline

fwd primer
type: SequenceAnnotation
persistent identity: annotation2154421
display id: annotation2154421
version: 1
top level: cheZ
location: https://synbiohub.org/public/igem/BBa_K629003/annotation2154421/range2154421/1
role: SO:0005850
role: feature/primer binding

https://synbiohub.org/public/igem/BBa_K629003/annotation2154421/range2154421/1
type: Range
persistent identity: range2154421
display id: range2154421
version: 1
top level: cheZ
direction: direction/forward
start: 1
end: 20
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2154422
display id: annotation2154422
version: 1
top level: cheZ
location: https://synbiohub.org/public/igem/BBa_K629003/annotation2154422/range2154422/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_K629003/annotation2154422/range2154422/1
type: Range
persistent identity: range2154422
display id: range2154422
version: 1
top level: cheZ
direction: direction/forward
start: 1
end: 3
orientation: inline

rev primer
type: SequenceAnnotation
persistent identity: annotation2154423
display id: annotation2154423
version: 1
top level: cheZ
location: https://synbiohub.org/public/igem/BBa_K629003/annotation2154423/range2154423/1
role: SO:0005850
role: feature/primer binding

https://synbiohub.org/public/igem/BBa_K629003/annotation2154423/range2154423/1
type: Range
persistent identity: range2154423
display id: range2154423
version: 1
top level: cheZ
direction: direction/forward
start: 624
end: 645
orientation: inline

TGA to TAA
type: SequenceAnnotation
persistent identity: annotation2155668
display id: annotation2155668
version: 1
top level: cheZ
location: https://synbiohub.org/public/igem/BBa_K629003/annotation2155668/range2155668/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_K629003/annotation2155668/range2155668/1
type: Range
persistent identity: range2155668
display id: range2155668
version: 1
top level: cheZ
direction: direction/forward
start: 643
end: 645
orientation: inline

terminator
type: SequenceAnnotation
persistent identity: annotation2154424
display id: annotation2154424
version: 1
top level: cheZ
location: https://synbiohub.org/public/igem/BBa_K629003/annotation2154424/range2154424/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_K629003/annotation2154424/range2154424/1
type: Range
persistent identity: range2154424
display id: range2154424
version: 1
top level: cheZ
direction: direction/forward
start: 643
end: 645
orientation: inline

BBa_K880005
type: ComponentDefinition
persistent identity: BBa K880005
display id: BBa_K880005
version: 1
was generated by: iGEM to SBOL conversion
description: Strong promoter, strong RBS combination for high expression levels of proteins
created: 2012-09-28T11:00:00Z
modified: 2015-05-08T01:13:40Z
mutable provenance: It is a combination of strong promoter (J23100) and RBS (B0034).
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: -J23100+B0034 -Strong promoter, strong RBS combination for high expression levels of proteins Consensus constitutive promoter and RBS sequence-produces strongest possible expression.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1142_
m user id: 0
owner id: 9403
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Enter design considerations.
star: false
creator: Josh Atkinson, Mike Ferguson, and Ben Parker
type: DnaRegion
role: partType/Generator
role: SO:0000804
component: BBa_B0034
component: BBa_J23100
sequence annotation: BBa_J23100
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K880005_sequence/1

BBa_B0034
type: Component
persistent identity: component2204230
display id: component2204230
version: 1
top level: BBa_K880005
definition: BBa_B0034
access: public

BBa_J23100
type: Component
persistent identity: component2204228
display id: component2204228
version: 1
top level: BBa_K880005
definition: BBa_J23100
access: public

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2204228
display id: annotation2204228
version: 1
top level: BBa_K880005
location: https://synbiohub.org/public/igem/BBa_K880005/annotation2204228/range2204228/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K880005/annotation2204228/range2204228/1
type: Range
persistent identity: range2204228
display id: range2204228
version: 1
top level: BBa_K880005
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2204230
display id: annotation2204230
version: 1
top level: BBa_K880005
location: https://synbiohub.org/public/igem/BBa_K880005/annotation2204230/range2204230/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K880005/annotation2204230/range2204230/1
type: Range
persistent identity: range2204230
display id: range2204230
version: 1
top level: BBa_K880005
direction: direction/reverse
start: 44
end: 55
orientation: inline

BBa_K1361998
type: ComponentDefinition
persistent identity: BBa K1361998
display id: BBa_K1361998
version: 1
was generated by: iGEM to SBOL conversion
description: Curli Fiber assembly associated protein CsgA, CsgC
created: 2014-10-02T11:00:00Z
modified: 2015-05-08T01:10:04Z
mutable provenance: PCR on genomic DNA from E coli. DH5alpha strain. And site-directed mutation by overlap PCR primers.
owned by: james
owned by: myers
mutable description: CsgA, CsgC genes are directly PCRed from E coli. DH5alpha genome as a whole. There is a scar sequence between CsgA and CsgC coding region that contains a promoter sequence may constitutively transcript CsgC gene. CsgA protein is the major subunit of Curli Fiber. CsgC may regulate CsgG outer membrane assembly of channel protein CsgG and pore activity through modification of C230 in CsgG.
discontinued: false
dominant: false
group u list: _1737_
m user id: 0
owner id: 16915
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: The PstI loci within CsgA sequence has been replaced from CTGCAG to CTGCCG.
star: false
creator: Shoujie Sun
type: DnaRegion
role: partType/Composite
role: SO:0000804
sequence annotation: CsgA
sequence annotation: start
sequence annotation: stop
sequence annotation: CsgC
sequence: https://synbiohub.org/public/igem/BBa_K1361998_sequence/1

CsgA
type: SequenceAnnotation
persistent identity: annotation2392512
display id: annotation2392512
version: 1
top level: BBa_K1361998
location: https://synbiohub.org/public/igem/BBa_K1361998/annotation2392512/range2392512/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K1361998/annotation2392512/range2392512/1
type: Range
persistent identity: range2392512
display id: range2392512
version: 1
top level: BBa_K1361998
direction: direction/forward
start: 1
end: 456
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2392513
display id: annotation2392513
version: 1
top level: BBa_K1361998
location: https://synbiohub.org/public/igem/BBa_K1361998/annotation2392513/range2392513/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_K1361998/annotation2392513/range2392513/1
type: Range
persistent identity: range2392513
display id: range2392513
version: 1
top level: BBa_K1361998
direction: direction/forward
start: 1
end: 3
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2392514
display id: annotation2392514
version: 1
top level: BBa_K1361998
location: https://synbiohub.org/public/igem/BBa_K1361998/annotation2392514/range2392514/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K1361998/annotation2392514/range2392514/1
type: Range
persistent identity: range2392514
display id: range2392514
version: 1
top level: BBa_K1361998
direction: direction/forward
start: 454
end: 456
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2392516
display id: annotation2392516
version: 1
top level: BBa_K1361998
location: https://synbiohub.org/public/igem/BBa_K1361998/annotation2392516/range2392516/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_K1361998/annotation2392516/range2392516/1
type: Range
persistent identity: range2392516
display id: range2392516
version: 1
top level: BBa_K1361998
direction: direction/forward
start: 515
end: 517
orientation: inline

CsgC
type: SequenceAnnotation
persistent identity: annotation2392515
display id: annotation2392515
version: 1
top level: BBa_K1361998
location: https://synbiohub.org/public/igem/BBa_K1361998/annotation2392515/range2392515/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K1361998/annotation2392515/range2392515/1
type: Range
persistent identity: range2392515
display id: range2392515
version: 1
top level: BBa_K1361998
direction: direction/forward
start: 515
end: 847
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2392517
display id: annotation2392517
version: 1
top level: BBa_K1361998
location: https://synbiohub.org/public/igem/BBa_K1361998/annotation2392517/range2392517/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_K1361998/annotation2392517/range2392517/1
type: Range
persistent identity: range2392517
display id: range2392517
version: 1
top level: BBa_K1361998
direction: direction/forward
start: 845
end: 847
orientation: inline

BBa_K1361997
type: ComponentDefinition
persistent identity: BBa K1361997
display id: BBa_K1361997
version: 1
was generated by: iGEM to SBOL conversion
description: CsgB, curlin nucleator protein, minor subunit in curli complex
created: 2014-10-02T11:00:00Z
modified: 2015-05-08T01:10:04Z
mutable provenance: PCR on genomic DNA from E coli. DH5alpha strain.
owned by: james
owned by: myers
mutable description: CsgB is a secretive protein that attached cells' outer membranes. It plays key role in curli fiber polymerization. CsgB is the nucleator for CsgA aggregation in vivo, that facilitate the transformation of soluble CsgA into polymer fiber.
discontinued: false
dominant: false
group u list: _1737_
m user id: 0
owner id: 16915
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: CsgB can be designed under inductively control while make CsgACEFG genes constitutively express respectly. Using this method could achieve the immediate synthesis of curli fiber after inducer has been added.
star: false
creator: Shoujie Sun
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: start
sequence annotation: CsgB
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_K1361997_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation2392539
display id: annotation2392539
version: 1
top level: BBa_K1361997
location: https://synbiohub.org/public/igem/BBa_K1361997/annotation2392539/range2392539/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_K1361997/annotation2392539/range2392539/1
type: Range
persistent identity: range2392539
display id: range2392539
version: 1
top level: BBa_K1361997
direction: direction/forward
start: 1
end: 3
orientation: inline

CsgB
type: SequenceAnnotation
persistent identity: annotation2392541
display id: annotation2392541
version: 1
top level: BBa_K1361997
location: https://synbiohub.org/public/igem/BBa_K1361997/annotation2392541/range2392541/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K1361997/annotation2392541/range2392541/1
type: Range
persistent identity: range2392541
display id: range2392541
version: 1
top level: BBa_K1361997
direction: direction/forward
start: 1
end: 456
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2392540
display id: annotation2392540
version: 1
top level: BBa_K1361997
location: https://synbiohub.org/public/igem/BBa_K1361997/annotation2392540/range2392540/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K1361997/annotation2392540/range2392540/1
type: Range
persistent identity: range2392540
display id: range2392540
version: 1
top level: BBa_K1361997
direction: direction/forward
start: 454
end: 456
orientation: inline

pBAD
type: ComponentDefinition
persistent identity: BBa K206000
display id: BBa_K206000
version: 1
was generated by: iGEM to SBOL conversion
description: pBAD strong
created: 2009-10-13T11:00:00Z
modified: 2015-05-08T01:11:23Z
mutable provenance: The sequence was obtained by applying all mutations that upregulated AraC binding and subsequent promoter activity listed in reference [1].
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: pBAD is an E.coli promoter that is induced by L-arabinose. K206000 is a mutagenized pBAD promoter that is responsive to lower concentrations of arabinose than wild type (<partinfo>I13453</partinfo>) and, additionally, exhibits a higher maximum of protein expression as measured by coupling to a fluorescent reporter.
discontinued: false
dominant: false
experience: experience/Works
group u list: _307_
m user id: 0
owner id: 4172
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: There were no special design considerations.
star: false
creator: Amelia Hardjasa
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: promoter
sequence annotation: AraI1
sequence annotation: AraI2
sequence: https://synbiohub.org/public/igem/BBa_K206000_sequence/1

promoter
type: SequenceAnnotation
persistent identity: annotation2049252
display id: annotation2049252
version: 1
top level: pBAD
location: https://synbiohub.org/public/igem/BBa_K206000/annotation2049252/range2049252/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K206000/annotation2049252/range2049252/1
type: Range
persistent identity: range2049252
display id: range2049252
version: 1
top level: pBAD
direction: direction/forward
start: 1
end: 131
orientation: inline

AraI1
type: SequenceAnnotation
persistent identity: annotation2049253
display id: annotation2049253
version: 1
top level: pBAD
location: https://synbiohub.org/public/igem/BBa_K206000/annotation2049253/range2049253/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_K206000/annotation2049253/range2049253/1
type: Range
persistent identity: range2049253
display id: range2049253
version: 1
top level: pBAD
direction: direction/forward
start: 40
end: 57
orientation: inline

AraI2
type: SequenceAnnotation
persistent identity: annotation2049254
display id: annotation2049254
version: 1
top level: pBAD
location: https://synbiohub.org/public/igem/BBa_K206000/annotation2049254/range2049254/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_K206000/annotation2049254/range2049254/1
type: Range
persistent identity: range2049254
display id: range2049254
version: 1
top level: pBAD
direction: direction/forward
start: 61
end: 78
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

cI lam
type: ComponentDefinition
persistent identity: BBa C0051
display id: BBa_C0051
version: 1
was generated by: iGEM to SBOL conversion
description: cI repressor from E. coli phage lambda (+LVA)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999).
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Coding region for the cI repressor based on cI repressor from bacteriophage lambda modified with an LVA tail for rapid degradation of the protein. cI repressor binds to the cI regulator (BBa_R0051).
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: References (unparsed) here: A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000 Octamerization of CI repressor is needed for effective repression of PRM and efficient switching from lysogeny. Ian B. Dodd,1 Alison J. Perkins, Daniel Tsemitsidis, and J. Barry Egan , Genes and Development (Vol 15, No. 22) 3013-3022: 2001 References (unparsed) here: A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000 Octamerization of CI repressor is needed for effective repression of PRM and efficient switching from lysogeny. Ian B. Dodd,1 Alison J. Perkins, Daniel Tsemitsidis, and J. Barry Egan , Genes and Development (Vol 15, No. 22) 3013-3022: 2001 BBa_C0051 cI repressor is based on the cI repressor from the Elowitz's repressilator. It has been modified to include a rapid degradation LAA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.
star: true
creator: Vinay S Mahajan, Brian Chow, Peter Carr, Voichita Marinescu and Alexander D. Wissner-Gross
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: cI lambda
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0051_sequence/1

cI lambda
type: SequenceAnnotation
persistent identity: annotation23334
display id: annotation23334
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_C0051/annotation23334/range23334/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_C0051/annotation23334/range23334/1
type: Range
persistent identity: range23334
display id: range23334
version: 1
top level: cI lam
direction: direction/forward
start: 4
end: 711
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation23335
display id: annotation23335
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_C0051/annotation23335/range23335/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_C0051/annotation23335/range23335/1
type: Range
persistent identity: range23335
display id: range23335
version: 1
top level: cI lam
direction: direction/forward
start: 712
end: 744
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213991
display id: annotation2213991
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_C0051/annotation2213991/range2213991/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_C0051/annotation2213991/range2213991/1
type: Range
persistent identity: range2213991
display id: range2213991
version: 1
top level: cI lam
direction: direction/forward
start: 751
end: 775
orientation: inline

BBa_J23100
type: ComponentDefinition
persistent identity: BBa J23100
display id: BBa_J23100
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-03T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: Isolated from library of promoters
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Replace later
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23100_sequence/1

https://synbiohub.org/public/igem/BBa_K1361000_sequence/1
type: Sequence
persistent identity: BBa K1361000 sequence
display id: BBa_K1361000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagaaagaggagaaatactagatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagatgaaaaacaaattgttatttatgatgttaacaatactgggtgcgcctgggattgcagccgcagcaggttatgatttagctaattcagaatataacttcgcggtaaatgaattgagtaagtcttcatttaatcaggcagccataattggtcaagctgggactaataatagtgctcagttacggcagggaggctcaaaacttttggcggttgttgcgcaagaaggtagtagcaaccgggcaaagattgaccagacaggagattataaccttgcatatattgatcaggcgggcagtgccaacgatgccagtatttcgcaaggtgcttatggtaatactgcgatgattatccagaaaggttctggtaataaagcaaatattacacagtatggtactcaaaaaacggcaattgtagtgcagagacagtcgcaaatggctattcgcgtgacacaacgttaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttgacggctagctcagtcctaggtacagtgctagctactagagaaagaggagaaatactagatgaaacttttaaaagtagcagcaattgcagcaatcgtattctccggtagcgctctggcaggtgttgttcctcagtacggcggcggcggtaaccacggtggtggcggtaataatagcggcccaaattctgagctgaacatttaccagtacggtggcggtaactctgcacttgctctgcaaactgatgcccgtaactctgacttgactattacccagcatggcggcggtaatggtgcagatgttggtcagggctcagatgacagctcaatcgatctgacccaacgtggcttcggtaacagcgctactcttgatcagtggaacggcaaaaattctgaaatgacggttaaacagttcggtggtggcaacggtgccgcagttgaccagactgcatctaactcctc…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0051_sequence/1
type: Sequence
persistent identity: BBa R0051 sequence
display id: BBa_R0051_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taacaccgtgcgtgttgactattttacctctggcggtgataatggttgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S0103_sequence/1
type: Sequence
persistent identity: BBa S0103 sequence
display id: BBa_S0103_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K629003_sequence/1
type: Sequence
persistent identity: BBa K629003 sequence
display id: BBa_K629003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgatgcaaccatcaatcaaacctgctgacgagcattcagctggcgatatcattgcgcgcatcggcagcctgacgcgtatgctgcgcgacagtttgcgggaactggggctggatcaggccattgccgaagcggcggaagccatccccgatgcgcgcgatcgtttgtactatgttgtgcagatgaccgcccaggctgcggagcgggcgctgaacagtgttgaggcgtcacaaccgcatcaggatcaaatggagaaatcagcaaaagcgttaacccaacgttgggatgactggtttgccgatccgattgaccttgccgacgcccgtgaactggtaacagatacacgacaatttctggcagatgtacccgcgcataccagctttactaacgcgcaactgctggaaatcatgatggcgcaggattttcaggatctcaccgggcaggtcattaagcggatgatggatgtcattcaggagatcgaacgccagttgctgatggtgctgttggaaaacatcccggaacaggagtcgcgtccaaaacgtgaaaaccagagtttgcttaatggacctcaggtcgataccagcaaagccggtgtggtagccagtcaggatcaggtggacgatttgttggatagtcttggattttaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K880005_sequence/1
type: Sequence
persistent identity: BBa K880005 sequence
display id: BBa_K880005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtacagtgctagctactagagaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1361998_sequence/1
type: Sequence
persistent identity: BBa K1361998 sequence
display id: BBa_K1361998_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaacttttaaaagtagcagcaattgcagcaatcgtattctccggtagcgctctggcaggtgttgttcctcagtacggcggcggcggtaaccacggtggtggcggtaataatagcggcccaaattctgagctgaacatttaccagtacggtggcggtaactctgcacttgctctgcaaactgatgcccgtaactctgacttgactattacccagcatggcggcggtaatggtgcagatgttggtcagggctcagatgacagctcaatcgatctgacccaacgtggcttcggtaacagcgctactcttgatcagtggaacggcaaaaattctgaaatgacggttaaacagttcggtggtggcaacggtgccgcagttgaccagactgcatctaactcctccgtcaacgtgactcaggttggctttggtaacaacgcgaccgctcatcagtactaatacatcatttgtattacagaaacagggcgcaagccctgttttttttcgggagaagaatatgaatacgttattactccttgcggcactttccagtcagataacctttaatacgacccagcaaggggatgtgtataccattattcctgaagtcactcttactcaatcttgtctgtgcagagtacaaatattgtccctgcgcgaaggcagttcagggcaaagtcagacgaagcaagaaaagaccctttcattgcctgctaatcaacccattgctttgacgaagttgagtttaaatatttccccggacgatcgggtgaaaatagttgttactgtttctgatggacagtcacttcatttatcacaacaatggccgccctcttcagaaaagtcttaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1361997_sequence/1
type: Sequence
persistent identity: BBa K1361997 sequence
display id: BBa_K1361997_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaaaacaaattgttatttatgatgttaacaatactgggtgcgcctgggattgcagccgcagcaggttatgatttagctaattcagaatataacttcgcggtaaatgaattgagtaagtcttcatttaatcaggcagccataattggtcaagctgggactaataatagtgctcagttacggcagggaggctcaaaacttttggcggttgttgcgcaagaaggtagtagcaaccgggcaaagattgaccagacaggagattataaccttgcatatattgatcaggcgggcagtgccaacgatgccagtatttcgcaaggtgcttatggtaatactgcgatgattatccagaaaggttctggtaataaagcaaatattacacagtatggtactcaaaaaacggcaattgtagtgcagagacagtcgcaaatggctattcgcgtgacacaacgttaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K206000_sequence/1
type: Sequence
persistent identity: BBa K206000 sequence
display id: BBa_K206000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0051_sequence/1
type: Sequence
persistent identity: BBa C0051 sequence
display id: BBa_C0051_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23100_sequence/1
type: Sequence
persistent identity: BBa J23100 sequence
display id: BBa_J23100_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtacagtgctagc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z