BBa_K1260005
type: ComponentDefinition
persistent identity: BBa K1260005
display id: BBa_K1260005
version: 1
was generated by: iGEM to SBOL conversion
description: pbad-ScFv (whole device)
created: 2014-05-23T11:00:00Z
modified: 2015-05-08T01:09:45Z
mutable provenance: It is constructed by four basic parts: BBa_K206001 BBa_B0030 BBa_K1260000 BBa_B0015.
owned by: myers
owned by: james
mutable description: It is a fully constructed device, made up of a L+arabinose weak promoter, a strong RBS, a ScFv coding sequence, and a double terminator.
discontinued: false
dominant: false
experience: experience/None
group u list: _1618_
m user id: 0
owner id: 19163
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: The expression of this device is very high, for the cds have been improved.
star: false
creator: Zhengfeng Liu
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K1260000
component: BBa_B0030
component: BBa_K206001
component: BBa_B0015
sequence annotation: BBa_K206001
sequence annotation: BBa_B0030
sequence annotation: BBa_K1260000
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K1260005_sequence/1

BBa_K1260000
type: Component
persistent identity: component2378298
display id: component2378298
version: 1
top level: BBa_K1260005
definition: BBa_K1260000
access: public

BBa_B0030
type: Component
persistent identity: component2378288
display id: component2378288
version: 1
top level: BBa_K1260005
definition: BBa_B0030
access: public

BBa_K206001
type: Component
persistent identity: component2378286
display id: component2378286
version: 1
top level: BBa_K1260005
definition: BBa_K206001
access: public

BBa_B0015
type: Component
persistent identity: component2378305
display id: component2378305
version: 1
top level: BBa_K1260005
definition: BBa_B0015
access: public

BBa_K206001
type: SequenceAnnotation
persistent identity: annotation2378286
display id: annotation2378286
version: 1
top level: BBa_K1260005
location: https://synbiohub.org/public/igem/BBa_K1260005/annotation2378286/range2378286/1
component: BBa_K206001

https://synbiohub.org/public/igem/BBa_K1260005/annotation2378286/range2378286/1
type: Range
persistent identity: range2378286
display id: range2378286
version: 1
top level: BBa_K1260005
direction: direction/reverse
start: 1
end: 130
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2378288
display id: annotation2378288
version: 1
top level: BBa_K1260005
location: https://synbiohub.org/public/igem/BBa_K1260005/annotation2378288/range2378288/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K1260005/annotation2378288/range2378288/1
type: Range
persistent identity: range2378288
display id: range2378288
version: 1
top level: BBa_K1260005
direction: direction/forward
start: 139
end: 153
orientation: inline

BBa_K1260000
type: SequenceAnnotation
persistent identity: annotation2378298
display id: annotation2378298
version: 1
top level: BBa_K1260005
location: https://synbiohub.org/public/igem/BBa_K1260005/annotation2378298/range2378298/1
component: BBa_K1260000

https://synbiohub.org/public/igem/BBa_K1260005/annotation2378298/range2378298/1
type: Range
persistent identity: range2378298
display id: range2378298
version: 1
top level: BBa_K1260005
direction: direction/reverse
start: 160
end: 1089
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2378305
display id: annotation2378305
version: 1
top level: BBa_K1260005
location: https://synbiohub.org/public/igem/BBa_K1260005/annotation2378305/range2378305/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1260005/annotation2378305/range2378305/1
type: Range
persistent identity: range2378305
display id: range2378305
version: 1
top level: BBa_K1260005
direction: direction/reverse
start: 1098
end: 1226
orientation: inline

BBa_B0030
type: ComponentDefinition
persistent identity: BBa B0030
display id: BBa_B0030
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.1 (strong) -- modified from R. Weiss
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _44_46_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS-1\Strong
sequence annotation: BBa_B0030
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0030_sequence/1

RBS-1\Strong
type: SequenceAnnotation
persistent identity: annotation1701
display id: annotation1701
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
type: Range
persistent identity: range1701
display id: range1701
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation7025
display id: annotation7025
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
type: Range
persistent identity: range7025
display id: range7025
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1702
display id: annotation1702
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
type: Range
persistent identity: range1702
display id: range1702
version: 1
top level: BBa_B0030
direction: direction/forward
start: 8
end: 12
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_K1260000
type: ComponentDefinition
persistent identity: BBa K1260000
display id: BBa_K1260000
version: 1
was generated by: iGEM to SBOL conversion
description: AFB1 ScFv with a his-tag, a SH3 ligand and a signal peptide
created: 2014-04-24T11:00:00Z
modified: 2015-05-08T01:09:45Z
mutable provenance: The part is constructed artificially.
owned by: james
owned by: myers
mutable description: AFB1 ScFv with a his-tag, a SH3 ligand and a signal peptide. The signal peptide is at the beginning of the sequence. With the part, you can let your E.coli produce antibody to target the aflatoxin B1.
discontinued: false
dominant: false
experience: experience/None
group u list: _1618_
m user id: 0
owner id: 19163
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: We have improved the codons of the coding sequence.
star: false
creator: Zhengfeng Liu
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: start
sequence annotation: ssDsbA
sequence annotation: linker 1
sequence annotation: AFB1-ScFv
sequence annotation: 6*his-tag
sequence annotation: linker 2
sequence annotation: SH3 Ligand
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_K1260000_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation2372753
display id: annotation2372753
version: 1
top level: BBa_K1260000
location: https://synbiohub.org/public/igem/BBa_K1260000/annotation2372753/range2372753/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_K1260000/annotation2372753/range2372753/1
type: Range
persistent identity: range2372753
display id: range2372753
version: 1
top level: BBa_K1260000
direction: direction/forward
start: 1
end: 3
orientation: inline

ssDsbA
type: SequenceAnnotation
persistent identity: annotation2372754
display id: annotation2372754
version: 1
top level: BBa_K1260000
location: https://synbiohub.org/public/igem/BBa_K1260000/annotation2372754/range2372754/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K1260000/annotation2372754/range2372754/1
type: Range
persistent identity: range2372754
display id: range2372754
version: 1
top level: BBa_K1260000
direction: direction/forward
start: 1
end: 69
orientation: inline

linker 1
type: SequenceAnnotation
persistent identity: annotation2372756
display id: annotation2372756
version: 1
top level: BBa_K1260000
location: https://synbiohub.org/public/igem/BBa_K1260000/annotation2372756/range2372756/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K1260000/annotation2372756/range2372756/1
type: Range
persistent identity: range2372756
display id: range2372756
version: 1
top level: BBa_K1260000
direction: direction/forward
start: 70
end: 87
orientation: inline

AFB1-ScFv
type: SequenceAnnotation
persistent identity: annotation2372755
display id: annotation2372755
version: 1
top level: BBa_K1260000
location: https://synbiohub.org/public/igem/BBa_K1260000/annotation2372755/range2372755/1
role: feature/protein
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K1260000/annotation2372755/range2372755/1
type: Range
persistent identity: range2372755
display id: range2372755
version: 1
top level: BBa_K1260000
direction: direction/forward
start: 88
end: 819
orientation: inline

6*his-tag
type: SequenceAnnotation
persistent identity: annotation2372757
display id: annotation2372757
version: 1
top level: BBa_K1260000
location: https://synbiohub.org/public/igem/BBa_K1260000/annotation2372757/range2372757/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_K1260000/annotation2372757/range2372757/1
type: Range
persistent identity: range2372757
display id: range2372757
version: 1
top level: BBa_K1260000
direction: direction/forward
start: 820
end: 837
orientation: inline

linker 2
type: SequenceAnnotation
persistent identity: annotation2372758
display id: annotation2372758
version: 1
top level: BBa_K1260000
location: https://synbiohub.org/public/igem/BBa_K1260000/annotation2372758/range2372758/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K1260000/annotation2372758/range2372758/1
type: Range
persistent identity: range2372758
display id: range2372758
version: 1
top level: BBa_K1260000
direction: direction/forward
start: 847
end: 891
orientation: inline

SH3 Ligand
type: SequenceAnnotation
persistent identity: annotation2372759
display id: annotation2372759
version: 1
top level: BBa_K1260000
location: https://synbiohub.org/public/igem/BBa_K1260000/annotation2372759/range2372759/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K1260000/annotation2372759/range2372759/1
type: Range
persistent identity: range2372759
display id: range2372759
version: 1
top level: BBa_K1260000
direction: direction/forward
start: 891
end: 924
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2372760
display id: annotation2372760
version: 1
top level: BBa_K1260000
location: https://synbiohub.org/public/igem/BBa_K1260000/annotation2372760/range2372760/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K1260000/annotation2372760/range2372760/1
type: Range
persistent identity: range2372760
display id: range2372760
version: 1
top level: BBa_K1260000
direction: direction/forward
start: 925
end: 930
orientation: inline

BBa_K206001
type: ComponentDefinition
persistent identity: BBa K206001
display id: BBa_K206001
version: 1
was generated by: iGEM to SBOL conversion
description: pBAD weak
created: 2009-10-16T11:00:00Z
modified: 2015-05-08T01:11:23Z
mutable provenance: Site-directed mutagenesis on <partinfo>I13453</partinfo> with the following primers: Forward: TAATCTTATGGATAAAAAAGCTATGGCATAGC Reverse: GCGGATCCTACCTGACGCTTTTTATC
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Weaker version of wild type pBAD (<partinfo>I13453</partinfo>).
discontinued: false
dominant: false
experience: experience/Works
group u list: _307_
m user id: 0
owner id: 4172
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: No special considerations
star: false
creator: Amelia Hardjasa
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: promoter
sequence annotation: AraI1
sequence annotation: AraI2
sequence: https://synbiohub.org/public/igem/BBa_K206001_sequence/1

promoter
type: SequenceAnnotation
persistent identity: annotation2049255
display id: annotation2049255
version: 1
top level: BBa_K206001
location: https://synbiohub.org/public/igem/BBa_K206001/annotation2049255/range2049255/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_K206001/annotation2049255/range2049255/1
type: Range
persistent identity: range2049255
display id: range2049255
version: 1
top level: BBa_K206001
direction: direction/forward
start: 1
end: 131
orientation: inline

AraI1
type: SequenceAnnotation
persistent identity: annotation2049256
display id: annotation2049256
version: 1
top level: BBa_K206001
location: https://synbiohub.org/public/igem/BBa_K206001/annotation2049256/range2049256/1
role: feature/operator
role: SO:0000057

https://synbiohub.org/public/igem/BBa_K206001/annotation2049256/range2049256/1
type: Range
persistent identity: range2049256
display id: range2049256
version: 1
top level: BBa_K206001
direction: direction/forward
start: 40
end: 57
orientation: inline

AraI2
type: SequenceAnnotation
persistent identity: annotation2049257
display id: annotation2049257
version: 1
top level: BBa_K206001
location: https://synbiohub.org/public/igem/BBa_K206001/annotation2049257/range2049257/1
role: feature/operator
role: SO:0000057

https://synbiohub.org/public/igem/BBa_K206001/annotation2049257/range2049257/1
type: Range
persistent identity: range2049257
display id: range2049257
version: 1
top level: BBa_K206001
direction: direction/forward
start: 61
end: 78
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

https://synbiohub.org/public/igem/BBa_K1260005_sequence/1
type: Sequence
persistent identity: BBa K1260005 sequence
display id: BBa_K1260005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acattgattatttgcacggcgtcacactttgctatgccatagcttttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagattaaagaggagaaatactagatgaaaaagacagctatcgcgattgcagtggcactggctggtttcgctaccgtagcgcaggccactagaggtagcggcagcggtagcactagagcacaggtgaaactgcaacagagcggaaccgaagttgttaaaccgggggcaagcgttaagctgagctgtaaggcaagcggttatatttttaccagctatgatattgattgggttcgtcagaccccggaacagggtctggaatggattggttggatttttccgggtgaaggtagcaccgaatataatgaaaaattcaaaggtcgtgcaaccctgagcgtagataaaagcagcagcaccgcatatatggaactgaccaggctgaccagcgaagatagcgcagtttatttttgtgcacgtggtgattattatcgtcgttattttgacctgtggggtcagggtaccaccgttaccgtttcctcaggtggaggcggttcaggcggaggtggctctggcggtggcggatcggatattgaactgacccagagcccggcaattatgagcgcaagcccgggtgaacgtgtcaccatgacctgtagcgcaagcagcagcattcgttatatttattggtatcagcagaaaccgggtagcagcccgcgtctgctgatttatgatacaagcaatgttgcaccgggtgttccttttcgttttagcggtagcggtagcggtaccagctatagcctgaccattaatcgtatggaagcagaagatgcagcaacctattattgtcaggaatggagcggttatccgtacacctttggtggtggtaccaaactggaactgaaacgtcatcatcatcatcatcacactagagctgaggccgccgcaaaagaagcagcagctaaggaagctgcggcgaagccccctccagcgctgccgccaaaacgtcgccggtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0030_sequence/1
type: Sequence
persistent identity: BBa B0030 sequence
display id: BBa_B0030_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1260000_sequence/1
type: Sequence
persistent identity: BBa K1260000 sequence
display id: BBa_K1260000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaaaagacagctatcgcgattgcagtggcactggctggtttcgctaccgtagcgcaggccactagaggtagcggcagcggtagcactagagcacaggtgaaactgcaacagagcggaaccgaagttgttaaaccgggggcaagcgttaagctgagctgtaaggcaagcggttatatttttaccagctatgatattgattgggttcgtcagaccccggaacagggtctggaatggattggttggatttttccgggtgaaggtagcaccgaatataatgaaaaattcaaaggtcgtgcaaccctgagcgtagataaaagcagcagcaccgcatatatggaactgaccaggctgaccagcgaagatagcgcagtttatttttgtgcacgtggtgattattatcgtcgttattttgacctgtggggtcagggtaccaccgttaccgtttcctcaggtggaggcggttcaggcggaggtggctctggcggtggcggatcggatattgaactgacccagagcccggcaattatgagcgcaagcccgggtgaacgtgtcaccatgacctgtagcgcaagcagcagcattcgttatatttattggtatcagcagaaaccgggtagcagcccgcgtctgctgatttatgatacaagcaatgttgcaccgggtgttccttttcgttttagcggtagcggtagcggtaccagctatagcctgaccattaatcgtatggaagcagaagatgcagcaacctattattgtcaggaatggagcggttatccgtacacctttggtggtggtaccaaactggaactgaaacgtcatcatcatcatcatcacactagagctgaggccgccgcaaaagaagcagcagctaaggaagctgcggcgaagccccctccagcgctgccgccaaaacgtcgccggtaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K206001_sequence/1
type: Sequence
persistent identity: BBa K206001 sequence
display id: BBa_K206001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acattgattatttgcacggcgtcacactttgctatgccatagcttttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z