LOCUS       BBa_K1260005            1226 bp    DNA     linear       24-Apr-2026
DEFINITION  pbad-ScFv (whole device)
ACCESSION   BBa_K1260005
VERSION     BBa_K1260005.1  
FEATURES             Location/Qualifiers
     promoter        1..131
                     /label=promoter
     misc_feature    40..57
                     /label=AraI1
     misc_feature    61..78
                     /label=AraI2
     promoter        1..130
                     /label=BBa_K206001
     RBS             139..153
                     /label=RBS-1\Strong
     RBS             146..150
                     /label=RBS
     misc_feature    139..153
                     /label=BBa_B0030
     RBS             139..153
                     /label=BBa_B0030
     misc_feature    160..162
                     /label=start
     misc_feature    160..228
                     /label=ssDsbA
     CDS             247..978
                     /label=AFB1-ScFv
     misc_feature    229..246
                     /label=linker 1
     misc_feature    979..996
                     /label=6*his-tag
     misc_feature    1006..1050
                     /label=linker 2
     misc_feature    1050..1083
                     /label=SH3 Ligand
     misc_feature    1084..1089
                     /label=stop
     CDS             160..1089
                     /label=BBa_K1260000
     stem_loop       1109..1152
                     /label=stem_loop
     misc_feature    1098..1177
                     /label=BBa_B0010
     terminator      1098..1177
                     /label=BBa_B0010
     stem_loop       1193..1212
                     /label=T7 TE
     misc_feature    1219..1219
                     /label=stop
     polyA_site      1213..1226
                     /label=polya
     misc_feature    1186..1226
                     /label=BBa_B0012
     terminator      1186..1226
                     /label=BBa_B0012
     terminator      1098..1226
                     /label=BBa_B0015
ORIGIN
        1 acattgatta tttgcacggc gtcacacttt gctatgccat agctttttta tccataagat
       61 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca taccgttttt
      121 ttgggctagc tactagagat taaagaggag aaatactaga tgaaaaagac agctatcgcg
      181 attgcagtgg cactggctgg tttcgctacc gtagcgcagg ccactagagg tagcggcagc
      241 ggtagcacta gagcacaggt gaaactgcaa cagagcggaa ccgaagttgt taaaccgggg
      301 gcaagcgtta agctgagctg taaggcaagc ggttatattt ttaccagcta tgatattgat
      361 tgggttcgtc agaccccgga acagggtctg gaatggattg gttggatttt tccgggtgaa
      421 ggtagcaccg aatataatga aaaattcaaa ggtcgtgcaa ccctgagcgt agataaaagc
      481 agcagcaccg catatatgga actgaccagg ctgaccagcg aagatagcgc agtttatttt
      541 tgtgcacgtg gtgattatta tcgtcgttat tttgacctgt ggggtcaggg taccaccgtt
      601 accgtttcct caggtggagg cggttcaggc ggaggtggct ctggcggtgg cggatcggat
      661 attgaactga cccagagccc ggcaattatg agcgcaagcc cgggtgaacg tgtcaccatg
      721 acctgtagcg caagcagcag cattcgttat atttattggt atcagcagaa accgggtagc
      781 agcccgcgtc tgctgattta tgatacaagc aatgttgcac cgggtgttcc ttttcgtttt
      841 agcggtagcg gtagcggtac cagctatagc ctgaccatta atcgtatgga agcagaagat
      901 gcagcaacct attattgtca ggaatggagc ggttatccgt acacctttgg tggtggtacc
      961 aaactggaac tgaaacgtca tcatcatcat catcacacta gagctgaggc cgccgcaaaa
     1021 gaagcagcag ctaaggaagc tgcggcgaag ccccctccag cgctgccgcc aaaacgtcgc
     1081 cggtaataat actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc
     1141 ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc
     1201 ttcgggtggg cctttctgcg tttata
//