LOCUS       BBa_K1230016            1741 bp    DNA     linear       13-Apr-2026
DEFINITION  pLacI-rbs-MarA-rbs-GFP-double terminator-pLsrA-rbs-LL-37
ACCESSION   BBa_K1230016
VERSION     BBa_K1230016.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    1..220
                     /label=BBa_J04500
     CDS             227..619
                     /label=MarA
     misc_feature    614..619
                     /label=stop
     misc_feature    227..229
                     /label=start
     misc_feature    358..358
                     /label=G to C mutation
     CDS             227..619
                     /label=BBa_K1230000
     misc_feature    1..619
                     /label=BBa_K1230004
     RBS             628..642
                     /label=RBS-1\Strong
     RBS             635..639
                     /label=RBS
     misc_feature    628..642
                     /label=BBa_B0030
     RBS             628..642
                     /label=BBa_B0030
     CDS             649..1368
                     /label=GFP protein
     CDS             649..1368
                     /label=BBa_E0040
     stem_loop       1388..1431
                     /label=stem_loop
     misc_feature    1377..1456
                     /label=BBa_B0010
     terminator      1377..1456
                     /label=BBa_B0010
     stem_loop       1472..1491
                     /label=T7 TE
     misc_feature    1498..1498
                     /label=stop
     polyA_site      1492..1505
                     /label=polya
     misc_feature    1465..1505
                     /label=BBa_B0012
     terminator      1465..1505
                     /label=BBa_B0012
     misc_feature    628..1505
                     /label=BBa_E0840
     RBS             1625..1636
                     /label=RBS BBa_B0034
     promoter        1514..1615
                     /label=BBa_K117002
     misc_feature    628..1615
                     /label=BBa_K1230008
     CDS             1622..1741
                     /label=LL-37
     misc_feature    1622..1624
                     /label=Start
     misc_feature    1735..1741
                     /label=Stop
     misc_feature    1622..1741
                     /label=BBa_K1230001
     misc_feature    628..1741
                     /label=BBa_K1230012
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatga cgatgtccag
      241 acgcaatact gacgctatta ccattcatag cattttggac tggatcgagg acaacctgga
      301 atcgccactg tcactggaga aagtgtcaga gcgttcgggt tactccaaat ggcacctcca
      361 acggatgttt aaaaaagaaa ccggtcattc attaggccaa tacatccgca gccgtaagat
      421 gacggaaatc gcgcaaaagc tgaaggaaag taacgagccg atactctatc tggcagaacg
      481 atatggcttc gagtcgcaac aaactctgac ccgaaccttc aaaaattact ttgatgttcc
      541 gccgcataaa taccggatga ccaatatgca gggcgaatcg cgctttttac atccattaaa
      601 tcattacaac agctaataat actagagatt aaagaggaga aatactagat gcgtaaagga
      661 gaagaacttt tcactggagt tgtcccaatt cttgttgaat tagatggtga tgttaatggg
      721 cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa catacggaaa acttaccctt
      781 aaatttattt gcactactgg aaaactacct gttccatggc caacacttgt cactactttc
      841 ggttatggtg ttcaatgctt tgcgagatac ccagatcata tgaaacagca tgactttttc
      901 aagagtgcca tgcccgaagg ttatgtacag gaaagaacta tatttttcaa agatgacggg
      961 aactacaaga cacgtgctga agtcaagttt gaaggtgata cccttgttaa tagaatcgag
     1021 ttaaaaggta ttgattttaa agaagatgga aacattcttg gacacaaatt ggaatacaac
     1081 tataactcac acaatgtata catcatggca gacaaacaaa agaatggaat caaagttaac
     1141 ttcaaaatta gacacaacat tgaagatgga agcgttcaac tagcagacca ttatcaacaa
     1201 aatactccaa ttggcgatgg ccctgtcctt ttaccagaca accattacct gtccacacaa
     1261 tctgcccttt cgaaagatcc caacgaaaag agagaccaca tggtccttct tgagtttgta
     1321 acagctgctg ggattacaca tggcatggat gaactataca aataataata ctagagccag
     1381 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt
     1441 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt
     1501 ttatatacta gagcacaaca tcaactgagt gcatgcctta tttaatcatg taactattct
     1561 tacgacttca aacgtcaaac agtcttaaca cttatttaat taaaaagagg agaaatacta
     1621 gatgctgctg ggtgatttct tccggaaatc taaagagaag attggcaaag agtttaaaag
     1681 aattgtccag agaatcaagg attttttgcg gaatcttgta cccaggacag agtcctaata
     1741 a
//