##gff-version 3
##sequence-region BBa_K1230013 1 2871
BBa_K1230013	.	engineered_region	1	2244	.	+	0	ID=BBa_K1230010;Name=BBa_K1230010
BBa_K1230013	.	engineered_region	1236	2244	.	+	0	ID=BBa_K1230003;Name=BBa_K1230003;Parent=BBa_K1230010
BBa_K1230013	.	CDS	1236	1358	.	+	0	ID=BBa_K875009;Name=BBa_K875009;Parent=BBa_K1230003
BBa_K1230013	.	start_codon	1236	1238	.	+	0	ID=annotation2200494;Name=start;Parent=BBa_K875009
BBa_K1230013	.	engineered_region	1367	2244	.	+	0	ID=BBa_E0840;Name=BBa_E0840;Parent=BBa_K1230003
BBa_K1230013	.	terminator	2204	2244	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0840
BBa_K1230013	.	polyA_site	2231	2244	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1230013	.	engineered_region	2204	2244	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1230013	.	stem_loop	2211	2230	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1230013	.	stop_codon	2237	2237	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1230013	.	ribosome_entry_site	1367	1381	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_E0840
BBa_K1230013	.	ribosome_entry_site	1367	1381	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K1230013	.	ribosome_entry_site	1374	1378	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K1230013	.	engineered_region	1367	1381	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K1230013	.	CDS	1388	2107	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_E0840
BBa_K1230013	.	CDS	1388	2107	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K1230013	.	terminator	2116	2195	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0840
BBa_K1230013	.	stem_loop	2127	2170	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1230013	.	engineered_region	2116	2195	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1230013	.	engineered_region	1	1229	.	+	0	ID=BBa_K1230009;Name=BBa_K1230009;Parent=BBa_K1230010
BBa_K1230013	.	ribosome_entry_site	1	12	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K1230009
BBa_K1230013	.	conserved_region	5	8	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1230013	.	promoter	21	1229	.	+	0	ID=BBa_K808000;Name=BBa_K808000;Parent=BBa_K1230009
BBa_K1230013	.	non_covalent_binding_site	929	944	.	+	0	ID=annotation2200758;Name=AraO;Parent=BBa_K808000
BBa_K1230013	.	non_covalent_binding_site	1086	1097	.	+	0	ID=annotation2200759;Name=AraO1;Parent=BBa_K808000
BBa_K1230013	.	CDS	21	899	.	+	0	ID=annotation2200757;Name=AraC;Parent=BBa_K808000
BBa_K1230013	.	promoter	1173	1201	.	+	0	ID=annotation2200760;Name=AraPromoter;Parent=BBa_K808000
BBa_K1230013	.	engineered_region	2253	2871	.	+	0	ID=BBa_K1230004;Name=BBa_K1230004
BBa_K1230013	.	CDS	2479	2871	.	+	0	ID=BBa_K1230000;Name=BBa_K1230000;Parent=BBa_K1230004
BBa_K1230013	.	point_mutation	2610	2610	.	+	0	ID=annotation2335368;Name=G to C mutation;Parent=BBa_K1230000
BBa_K1230013	.	stop_codon	2866	2871	.	+	0	ID=annotation2335366;Name=stop;Parent=BBa_K1230000
BBa_K1230013	.	CDS	2479	2871	.	+	0	ID=annotation2335365;Name=MarA;Parent=BBa_K1230000
BBa_K1230013	.	start_codon	2479	2481	.	+	0	ID=annotation2335367;Name=start;Parent=BBa_K1230000
BBa_K1230013	.	engineered_region	2253	2472	.	+	0	ID=BBa_J04500;Name=BBa_J04500;Parent=BBa_K1230004
BBa_K1230013	.	ribosome_entry_site	2461	2472	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J04500
BBa_K1230013	.	conserved_region	2465	2468	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1230013	.	promoter	2253	2452	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_J04500
BBa_K1230013	.	start_codon	2425	2425	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K1230013	.	CDS	2253	2340	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K1230013	.	promoter	2413	2418	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K1230013	.	non_covalent_binding_site	2418	2452	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K1230013	.	non_covalent_binding_site	2341	2378	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K1230013	.	engineered_region	2253	2452	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K1230013	.	promoter	2389	2394	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
>BBa_K1230013
aaagaggagaaatactagagttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgc
tcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggt
ggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgcta
atccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaa
aattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactc
gttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttcccct
tgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtat
tggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattc
gcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaat
tctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggt
cgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccgg
cagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgc
atcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattc
tgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattga
ttatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgcttttta
tcgcaactctctactgtttctccatacccgttttttgggctaactactagatgctgctgggcgatttttttcgcaaaag
caaagaaaaaattggcaaagaatttaaacgcattgtgcagcgcattaaagattttctgcgcaacctggtgccgcgcacc
gaaagctaataataatactagagattaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgt
cccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaaca
tacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcg
gttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaagg
ttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgat
acccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaataca
actataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaa
cattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttacca
gacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagt
ttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaac
gaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactgg
ctcaccttcgggtgggcctttctgcgtttatatactagagcaatacgcaaaccgcctctccccgcgcgttggccgattc
attaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcac
tcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcac
acatactagagaaagaggagaaatactagatgacgatgtccagacgcaatactgacgctattaccattcatagcatttt
ggactggatcgaggacaacctggaatcgccactgtcactggagaaagtgtcagagcgttcgggttactccaaatggcac
ctccaacggatgtttaaaaaagaaaccggtcattcattaggccaatacatccgcagccgtaagatgacggaaatcgcgc
aaaagctgaaggaaagtaacgagccgatactctatctggcagaacgatatggcttcgagtcgcaacaaactctgacccg
aaccttcaaaaattactttgatgttccgccgcataaataccggatgaccaatatgcagggcgaatcgcgctttttacat
ccattaaatcattacaacagctaataa