LOCUS       BBa_I13501               843 bp    DNA     linear       05-Apr-2026
DEFINITION  Screening plasmid intermediate
ACCESSION   BBa_I13501
VERSION     BBa_I13501.1  
FEATURES             Location/Qualifiers
     CDS             1..675
                     /label=mrfp1
     misc_feature    682..706
                     /label=Help:Barcodes
     CDS             1..681
                     /label=BBa_E1010
     stem_loop       726..769
                     /label=stem_loop
     misc_feature    715..794
                     /label=BBa_B0010
     terminator      715..794
                     /label=BBa_B0010
     stem_loop       810..829
                     /label=T7 TE
     misc_feature    836..836
                     /label=stop
     polyA_site      830..843
                     /label=polya
     misc_feature    803..843
                     /label=BBa_B0012
     terminator      803..843
                     /label=BBa_B0012
ORIGIN
        1 atggcttcct ccgaagacgt tatcaaagag ttcatgcgtt tcaaagttcg tatggaaggt
       61 tccgttaacg gtcacgagtt cgaaatcgaa ggtgaaggtg aaggtcgtcc gtacgaaggt
      121 acccagaccg ctaaactgaa agttaccaaa ggtggtccgc tgccgttcgc ttgggacatc
      181 ctgtccccgc agttccagta cggttccaaa gcttacgtta aacacccggc tgacatcccg
      241 gactacctga aactgtcctt cccggaaggt ttcaaatggg aacgtgttat gaacttcgaa
      301 gacggtggtg ttgttaccgt tacccaggac tcctccctgc aagacggtga gttcatctac
      361 aaagttaaac tgcgtggtac caacttcccg tccgacggtc cggttatgca gaaaaaaacc
      421 atgggttggg aagcttccac cgaacgtatg tacccggaag acggtgctct gaaaggtgaa
      481 atcaaaatgc gtctgaaact gaaagacggt ggtcactacg acgctgaagt taaaaccacc
      541 tacatggcta aaaaaccggt tcagctgccg ggtgcttaca aaaccgacat caaactggac
      601 atcacctccc acaacgaaga ctacaccatc gttgaacagt acgaacgtgc tgaaggtcgt
      661 cactccaccg gtgcttaata acgctgatag tgctagtgta gatcgctact agagccaggc
      721 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
      781 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt
      841 ata
//
LOCUS       BBa_K1197009             961 bp    DNA     linear       05-Apr-2026
DEFINITION  Phyp + RBS (SpoVG) + RFP + DT
ACCESSION   BBa_K1197009
VERSION     BBa_K1197009.1  
FEATURES             Location/Qualifiers
     misc_feature    1..21
                     /label=Lac0
     promoter        37..41
                     /label=-35
     promoter        60..65
                     /label=-10
     misc_feature    72..92
                     /label=LacO
     promoter        1..92
                     /label=BBa_K606040
     RBS             101..112
                     /label=rbs
     RBS             101..112
                     /label=BBa_K143021
     CDS             119..793
                     /label=mrfp1
     misc_feature    800..824
                     /label=Help:Barcodes
     CDS             119..799
                     /label=BBa_E1010
     stem_loop       844..887
                     /label=stem_loop
     misc_feature    833..912
                     /label=BBa_B0010
     terminator      833..912
                     /label=BBa_B0010
     stem_loop       928..947
                     /label=T7 TE
     misc_feature    954..954
                     /label=stop
     polyA_site      948..961
                     /label=polya
     misc_feature    921..961
                     /label=BBa_B0012
     terminator      921..961
                     /label=BBa_B0012
     terminator      833..961
                     /label=BBa_B0015
     CDS             119..961
                     /label=BBa_K606012
     misc_feature    101..961
                     /label=BBa_K606013
ORIGIN
        1 aaatgtgagc actcacaatt cattttgcaa aagttgttga ctttatctac aaggtgtggc
       61 ataatgtgtg taattgtgag cggataacaa tttactagag aaaggtggtg aatactagat
      121 ggcttcctcc gaagacgtta tcaaagagtt catgcgtttc aaagttcgta tggaaggttc
      181 cgttaacggt cacgagttcg aaatcgaagg tgaaggtgaa ggtcgtccgt acgaaggtac
      241 ccagaccgct aaactgaaag ttaccaaagg tggtccgctg ccgttcgctt gggacatcct
      301 gtccccgcag ttccagtacg gttccaaagc ttacgttaaa cacccggctg acatcccgga
      361 ctacctgaaa ctgtccttcc cggaaggttt caaatgggaa cgtgttatga acttcgaaga
      421 cggtggtgtt gttaccgtta cccaggactc ctccctgcaa gacggtgagt tcatctacaa
      481 agttaaactg cgtggtacca acttcccgtc cgacggtccg gttatgcaga aaaaaaccat
      541 gggttgggaa gcttccaccg aacgtatgta cccggaagac ggtgctctga aaggtgaaat
      601 caaaatgcgt ctgaaactga aagacggtgg tcactacgac gctgaagtta aaaccaccta
      661 catggctaaa aaaccggttc agctgccggg tgcttacaaa accgacatca aactggacat
      721 cacctcccac aacgaagact acaccatcgt tgaacagtac gaacgtgctg aaggtcgtca
      781 ctccaccggt gcttaataac gctgatagtg ctagtgtaga tcgctactag agccaggcat
      841 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg
      901 gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat
      961 a
//