LOCUS BBa_K1152004 6132 bp DNA linear 29-Apr-2026 DEFINITION Expression cassette for NRPS that synthesizes a Pro-Leu-Dipeptide ACCESSION BBa_K1152004 VERSION BBa_K1152004.1 FEATURES Location/Qualifiers promoter 162..167 /label=-10 promoter 138..143 /label=-35 misc_feature 174..174 /label=start CDS 228..2090 /label=TycB1dComdC CDS 2091..5963 /label=TycC6 misc_feature 6098..6117 /label=T7 TE misc_feature 6124..6124 /label=stop ORIGIN 1 gcaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac 61 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact 121 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg 181 agcggataac aatttcacac atactagaga aagaggagaa atactagatg gattgcgtgg 241 caaacaattc gggagtcgag ctttgccaga ttccgttgct gacagaagca gaaactagcc 301 agctgttggc aaagcgtacg gaaacagcgg ctgactatcc tgccgcaacc atgcacgagc 361 tgttttcgcg gcaggcagaa aaaacgcctg agcaagtggc ggtagtcttc gcggatcagc 421 acctgacgta tcgggagctg gatgaaaaat ccaatcagct cgcccgcttt ttgcgcaaaa 481 aaggcattgg cacgggcagt cttgtcggca cgctgctgga tcgctcgctg gacatgatcg 541 tcggaatcct cggcgtcttg aaggcaggcg gcgcatttgt gccgatcgac ccggagttgc 601 ctgccgaacg aatcgcttac atgctgacgc atagcagagt tccattggtc gtgacgcaaa 661 atcatttgcg ggcaaaagtg accacgccta cagaaacaat tgacatcaac acagcggtga 721 tcggggaaga gagccgcgcc cctatcgaat cgctcaatca gccgcatgac ttgttttaca 781 tcatctatac gtccggaacg acagggcaac cgaaaggcgt catgctggag catcgcaaca 841 tggcgaacct gatgcatttt acgtttgatc agacgaacat cgcttttcat gaaaaagtgt 901 tgcagtatac cacgtgcagc tttgatgttt gctaccagga aattttctcc acgctgctat 961 ccgggggcca gctctacctg atcacgaacg agctgagacg gcatgtggaa aagctgtttg 1021 ctttcatcca ggaaaagcag atcagcattt tgtctctccc ggtgtccttc ctgaaattta 1081 tttttaacga acaagactac gcgcaaagct tcccgcgttg tgtcaaacat atcatcacgg 1141 ccggggaaca actcgtcgtc acacacgagc tgcaaaagta tctgcgccag catcgcgtat 1201 ttttgcacaa tcactacggc ccgtcggaga cgcatgtggt gacgacatgc acgatggacc 1261 cgggacaggc gataccagag ctgccgccca tcggaaagcc gatcagcaac acaggcattt 1321 acattttgga tgaagggctg caattgaagc cggaggggat cgtcggggag ttgtacattt 1381 ccggcgcaaa cgtaggaaga gggtatttgc accagccgga gctgaccgcg gagaagtttc 1441 tcgacaatcc gtatcagcca ggcgaaagaa tgtaccgaac gggtgatctg gccctttggt 1501 tgccggatgg ccagctcgaa tttttgggcc gaatcgacca tcaggtaaaa atcaggggcc 1561 atcgcatcga gctgggagag atcgaatcgc gcctgctcaa ccatcccgcc atcaaggaag 1621 cggtggttat cgaccgagca gacgagacag gcggcaagtt tttgtgcgcc tatgtcgtcc 1681 tgcaaaaagc gctcagcgac gaagagatgc gggcatactt ggcgcaagcg ttgccggagt 1741 atatgatccc ttcctttttc gtgacgctgg agcggattcc agtcacgccg aacggaaaaa 1801 cagacaggcg agctttgccg aagccggaag gaagtgccaa gacgaaagcg gattacgtcg 1861 ccccgacgac tgagctggaa caaaagctgg tcgcgatttg ggagcaaatt cttggcgtgt 1921 cgccgatcgg cattcaggat cattttttca cgctgggcgg ccattcgtta aaagcgattc 1981 agctcatttc ccgcatccaa aaggaatgcc aggcggatgt cccgctgcgc gtcctgtttg 2041 agcaaccgac gattcaagcg ctggcagcgt atgtggaagg cggggaggaa gggaatgtct 2101 tctcgatcga gcctgtgcaa aagcaagcgt actatccggt ctcctcggca caaaagcgca 2161 tgtacatcct cgatcaattt gagggagtcg gcatcagcta caacatgccg tcgactatgc 2221 tgatcgaagg caagctggag cgaacacggg tagaagcggc gttccagcgc ttgattgcgc 2281 gacatgaaag cctgcgcact tcgtttgccg tcgtcaacgg agagcctgtg caaaacattc 2341 acgaggacgt tccgtttgcg cttgcctatt cggaagtcac agaacaggag gcgcgcgaac 2401 tcgtttcttc tctcgtgcag ccgttcgatc tggaggtcgc accactcatc cgcgtgtcgc 2461 tgctgaaaat cggcgaggat cgttacgtgc tctttaccga catgcatcac agcatttccg 2521 atggcgtatc ctccggcatt cttttggcag agtgggtgca gctgtaccag ggtgacgttt 2581 tgccggagct gcgtatccag tacaaggact ttgctgtgtg gcaacaagag ttttcccagt 2641 cggctgcctt ccacaagcag gaagcgtact ggttgcaaac gtttgccgat gacattcctg 2701 tgctgaactt gccgaccgat ttcacccgcc ccagcaccca aagctttgcc ggggatcagt 2761 gcacgatcgg cgcgggcaaa gcgctcacgg aaggcttgca ccagttggcg caggcgacgg 2821 gaacgacttt gtacatggtt ttgctcgccg cgtacaacgt gctgctcgcc aagtatgccg 2881 ggcaggagga catcatcgtc ggcacgccga ttacaggcag atcccatgcc gatctcgaac 2941 cgatcgtcgg catgttcgtg aacaccttgg cgatgcgaaa caaaccgcag cgcgaaaaga 3001 cttttagcga gtttttgcaa gaagtcaagc aaaatgcgct ggatgcgtac ggccatcagg 3061 attacccgtt tgaagaactg gtggaaaagc tcgcgatcgc gcgcgatttg agccgaaatc 3121 cgctgtttga caccgtgttt acgttccaaa acagcacgga agaggtcatg acgctgcctg 3181 aatgcacgct tgcgccgttt atgacggacg aaacaggcca gcacgccaag ttcgacttga 3241 ctttcagcgc tacggaagag cgggaagaaa tgacgattgg cgtggagtac agcacaagct 3301 tgtttacgcg ggaaacgatg gaacggttca gccgccactt cctgacgatt gcagcgagca 3361 tcgtgcaaaa tccgcacatc cgtctgggcg agatcgacat gcttttgcca gaagaaaaac 3421 agcagatttt ggccgggttc aacgatacgg cagtcagcta tgcgctggac aaaacgctgc 3481 accagctatt cgaagagcag gtcgacaaaa caccggatca ggcagcgctt ctctttagcg 3541 agcaatcgct gacgtacagc gaactgaacg agcgagcaaa cagactggca agggtcctgc 3601 gcgcaaaagg agtcggaccg gaccgtctgg tagcgatcat ggcggagcgc tcgccggaaa 3661 tggtgatcgg tattctcggt attttgaagg caggcggcgc ttatgttccc gtcgatcccg 3721 gctatccgca ggagcgcatt cagtacctgc tcgaagatag caacgcagcc ctgctgctca 3781 gccaggcgca tctgttgccg ctgttggccc aggtgtcaag cgagctgccg gagtgccttg 3841 atctgaacgc tgaactggat gccggactga gcggctccaa cctgccagct gtcaaccaac 3901 cgactgacct tgcctacgtc atctatacat ccggtacgac cggcaagccg aagggtgtca 3961 tgatcccgca tcaaggaatc gtgaactgct tgcagtggag aagagacgaa tacgggttcg 4021 ggccgagtga caaggcgttg caagtgttct cctttgcctt cgacggtttt gtagccagct 4081 tgttcgctcc gctgctcgga ggggcaacgt gcgtgttgcc gcaagaagca gctgccaaag 4141 acccggtcgc gctgaaaaaa ctgatggccg caacggaagt cacccattac tacggcgtac 4201 cgagtctgtt ccaggccatt ctcgattgct cgacgacaac cgacttcaat cagttgcgtt 4261 gcgtcacttt gggcggcgag aagctgcctg tgcagcttgt gcaaaaaaca aaagaaaagc 4321 atccggcaat cgagatcaac aacgagtacg gcccgacgga aaacagcgtc gtcaccacca 4381 tctcgcgctc gattgaagcg gggcaagcga tcacgattgg ccgaccgctt gcgaacgtcc 4441 aagtctacat tgtagatgag cagcatcact tgcagccgat tggcgtggtc ggtgagctgt 4501 gcatcggcgg agccgggctt gccagaggct atctgaacaa accggagctg accgcagaga 4561 agtttgtcgc aaatccgttc cgaccaggcg agcgcatgta caaaacaggc gacttggtaa 4621 aatggcggac ggatggcacg atcgagtaca tcggccgcgc agacgaacag gtcaaggtga 4681 gagggtatcg catcgagatc ggcgagatcg agagcgccgt actcgcttac cagggcatcg 4741 atcaagcggt ggtcgttgcg cgagacgatg acgctacggc tggttcctat ctttgcgcct 4801 actttgtcgc agcaacagcc gtgtccgtat ccggcttgag aagccatctg gccaaagagc 4861 tgcctgctta catgattccg agctatttcg tcgagctgga tcagctgccg ctttccgcca 4921 atggaaaagt ggatcgcaaa gctttgccga agccgcaaca gtccgatgcg accacgcgcg 4981 aatacgtggc cccgaggaat gcgaccgaac agcaactggc agccatctgg caagaagttt 5041 tgggagtaga gccaatcggc atcaccgacc agttctttga actcggagga cattccttaa 5101 aagctacgct gttgattgcc aaagtgtatg agtacatgca aatcgagctg ccgctgaatc 5161 tcatcttcca gtatccgacg atcgaaaagg tggccgattt catcacgcat aagcgctttg 5221 agagcagata cggcacagcc attttgttaa atcaggagac ggcgcgaaac gtattttgct 5281 tcacgccgat cggcgcacaa agcgtgtact accagaagct tgcggcggaa attcaaggcg 5341 tctctttgta cagctttgat ttcatccagg atgacaaccg gatggagcag tatatcgcgg 5401 cgatcaccgc aattgatcca agcggtccgt acacgctcat gggctactcc tcgggaggca 5461 atctggcttt tgaagtggcg aaagaactgg aggagcgggg ctatggcgtc accgacatca 5521 tcttgttcga ctcgtactgg aaagacaagg cgattgagcg gactgtcgcg gaaacagaaa 5581 acgacattgc ccagctattc gccgagattg gcgaaaacac cgagatgttc aacatgacgc 5641 aagaagactt ccagctgtac gccgccaatg agtttgtcaa gcaaagcttc gttcgcaaaa 5701 cggtcagcta tgtgatgttc cataacaatc tggtcaatac cggaatgacc actgccgcga 5761 tccacctcat ccaatccgag ctggaagcag acgaggaagc tccggtggca gccaagtgga 5821 acgaatcagc ctgggcaaac gcaacgcaac gactgctgac gtacagcggg cacggaatcc 5881 actcgcgcat gctggcgggc gattacgcgt cgcaaaatgc ttcgattttg caaaacatcc 5941 tgcaagaact gttcatcctg aaataataac gctgatagtg ctagtgtaga tcgctactag 6001 agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg 6061 ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt 6121 ctgcgtttat at //