LOCUS       BBa_K1151013             545 bp    DNA     linear       02-Mar-2026
DEFINITION  Hpn coding device (BBa_J04500 + BBa_K1151001 + BBa_B0015)
ACCESSION   BBa_K1151013
VERSION     BBa_K1151013.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    1..220
                     /label=BBa_J04500
     CDS             227..408
                     /label=BBa_K1151001
     stem_loop       428..471
                     /label=stem_loop
     misc_feature    417..496
                     /label=BBa_B0010
     terminator      417..496
                     /label=BBa_B0010
     stem_loop       512..531
                     /label=T7 TE
     misc_feature    538..538
                     /label=stop
     polyA_site      532..545
                     /label=polya
     misc_feature    505..545
                     /label=BBa_B0012
     terminator      505..545
                     /label=BBa_B0012
     terminator      417..545
                     /label=BBa_B0015
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgg cacaccatga
      241 agaacaacac ggcggacacc accaccatca ccatcacaac accaccacca ctaccatggc
      301 ggcgaacacc accatcacca ccacagctct catcatgaag aaggttgttg tagcactagc
      361 gatagtcatc atcaagaaga aggttgctgc cacgggcatc acgagtaata ctagagccag
      421 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt
      481 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt
      541 ttata
//