LOCUS       BBa_K1140002             964 bp    DNA     linear       12-Mar-2026
DEFINITION  pLac + 32 oC RNA thermometer + GFP(LVA)
ACCESSION   BBa_K1140002
VERSION     BBa_K1140002.1  
FEATURES             Location/Qualifiers
     promoter        1..56
                     /label=pLac
     stem_loop       65..147
                     /label=RNA thermometer
     CDS             154..912
                     /label=GFP+LVA
     stem_loop       920..964
                     /label=T7 terminator
ORIGIN
        1 cgttgacacc atcgaatggc gcaaaacctt tcgcggtatg gcatgatagc gcccggtact
       61 agagccgggc gcccttcggg ggcccggcgg agacgggcgc cggaggtgtc cgacgcctgc
      121 tcgtccagtc tttgctcagt ggaggattac tagatgcgta aaggagaaga acttttcact
      181 ggagttgtcc caattcttgt tgaattagat ggtgatgtta atgggcacaa attttctgtc
      241 agtggagagg gtgaaggtga tgcaacatac ggaaaactta cccttaaatt tatttgcact
      301 actggaaaac tacctgttcc atggccaaca cttgtcacta ctttcggtta tggtgttcaa
      361 tgctttgcga gatacccaga tcatatgaaa cagcatgact ttttcaagag tgccatgccc
      421 gaaggttatg tacaggaaag aactatattt ttcaaagatg acgggaacta caagacacgt
      481 gctgaagtca agtttgaagg tgataccctt gttaatagaa tcgagttaaa aggtattgat
      541 tttaaagaag atggaaacat tcttggacac aaattggaat acaactataa ctcacacaat
      601 gtatacatca tggcagacaa acaaaagaat ggaatcaaag ttaacttcaa aattagacac
      661 aacattgaag atggaagcgt tcaactagca gaccattatc aacaaaatac tccaattggc
      721 gatggccctg tccttttacc agacaaccat tacctgtcca cacaatctgc cctttcgaaa
      781 gatcccaacg aaaagagaga ccacatggtc cttcttgagt ttgtaacagc tgctgggatt
      841 acacatggca tggatgaact atacaaaagg cctgctgcaa acgacgaaaa ctacgcttta
      901 gtagcttaat aatactagag cataacccct tggggcctct aaacgggtct tgaggggttt
      961 tttg
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