##gff-version 3
##sequence-region BBa_K1135003 1 1003
BBa_K1135003	.	promoter	1	131	.	+	0	ID=BBa_K118011;Name=BBa_K118011
BBa_K1135003	.	non_covalent_binding_site	1	42	.	+	0	ID=annotation1972435;Name=cAMP receptor protein binding site;Parent=BBa_K118011
BBa_K1135003	.	non_covalent_binding_site	101	131	.	+	0	ID=annotation1972436;Name=RNA polymerase binding site;Parent=BBa_K118011
BBa_K1135003	.	engineered_region	140	1003	.	+	0	ID=BBa_K516030;Name=BBa_K516030
BBa_K1135003	.	terminator	875	1003	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K516030
BBa_K1135003	.	terminator	875	954	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1135003	.	stem_loop	886	929	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1135003	.	engineered_region	875	954	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1135003	.	terminator	963	1003	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1135003	.	stem_loop	970	989	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1135003	.	stop_codon	996	996	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1135003	.	engineered_region	963	1003	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1135003	.	polyA_site	990	1003	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1135003	.	ribosome_entry_site	140	154	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_K516030
BBa_K1135003	.	engineered_region	140	154	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K1135003	.	ribosome_entry_site	147	151	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K1135003	.	ribosome_entry_site	140	154	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K1135003	.	CDS	161	866	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_K516030
BBa_K1135003	.	engineered_tag	842	866	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K1135003	.	CDS	161	835	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
>BBa_K1135003
actcggttaacggagtgatcgagttaacattgttaagttaaatattggtttcaactccgatttacatggttgctgtgtt
gttaaattgtacaaagatgttatagaaacaaaatgtaacatctctatggacatactagagattaaagaggagaaatact
agatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacga
gttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggt
ccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgaca
tcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgt
taccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtcc
gacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctga
aaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaa
aaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatc
gttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctac
tagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaac
gctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata