##gff-version 3
##sequence-region BBa_K1022125 1 4876
BBa_K1022125	.	engineered_region	1	1260	.	+	0	ID=BBa_K1022105;Name=BBa_K1022105
BBa_K1022125	.	engineered_region	421	1260	.	+	0	ID=BBa_P0440;Name=BBa_P0440;Parent=BBa_K1022105
BBa_K1022125	.	terminator	1132	1211	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_P0440
BBa_K1022125	.	engineered_region	1132	1211	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1022125	.	stem_loop	1143	1186	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1022125	.	ribosome_entry_site	421	432	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_P0440
BBa_K1022125	.	conserved_region	425	428	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1022125	.	CDS	439	1098	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_P0440
BBa_K1022125	.	CDS	442	1058	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K1022125	.	tag	1059	1092	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K1022125	.	engineered_tag	1099	1123	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K1022125	.	terminator	1220	1260	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_P0440
BBa_K1022125	.	engineered_region	1220	1260	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1022125	.	polyA_site	1247	1260	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1022125	.	stem_loop	1227	1246	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1022125	.	stop_codon	1253	1253	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1022125	.	sequence_feature	1	412	.	+	0	ID=BBa_K1022102;Name=BBa_K1022102;Parent=BBa_K1022105
BBa_K1022125	.	tag	43	363	.	+	0	ID=annotation2334996;Name=His6:SUMO;Parent=BBa_K1022102
BBa_K1022125	.	ribosome_entry_site	29	42	.	+	0	ID=annotation2334995;Name=34;Parent=BBa_K1022102
BBa_K1022125	.	sequence_feature	364	412	.	+	0	ID=annotation2334997;Name=Signiferin;Parent=BBa_K1022102
BBa_K1022125	.	promoter	1	28	.	+	0	ID=annotation2334994;Name=T7;Parent=BBa_K1022102
BBa_K1022125	.	engineered_region	1269	4876	.	+	0	ID=BBa_K1022123;Name=BBa_K1022123
BBa_K1022125	.	engineered_region	1269	3083	.	+	0	ID=BBa_K1022115;Name=BBa_K1022115;Parent=BBa_K1022123
BBa_K1022125	.	sequence_feature	2269	3083	.	+	0	ID=BBa_K1022107;Name=BBa_K1022107;Parent=BBa_K1022115
BBa_K1022125	.	promoter	2269	2317	.	+	0	ID=annotation2335074;Name=cI;Parent=BBa_K1022107
BBa_K1022125	.	ribosome_entry_site	2326	2342	.	+	0	ID=annotation2335075;Name=34;Parent=BBa_K1022107
BBa_K1022125	.	sequence_feature	2343	3083	.	+	0	ID=annotation2335076;Name=Ulp;Parent=BBa_K1022107
BBa_K1022125	.	engineered_region	1269	2260	.	+	0	ID=BBa_K1022108;Name=BBa_K1022108;Parent=BBa_K1022115
BBa_K1022125	.	terminator	2132	2260	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K1022108
BBa_K1022125	.	terminator	2220	2260	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1022125	.	engineered_region	2220	2260	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1022125	.	polyA_site	2247	2260	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1022125	.	stem_loop	2227	2246	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1022125	.	stop_codon	2253	2253	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1022125	.	terminator	2132	2211	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1022125	.	engineered_region	2132	2211	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1022125	.	stem_loop	2143	2186	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1022125	.	promoter	1269	1342	.	+	0	ID=BBa_J13002;Name=BBa_J13002;Parent=BBa_K1022108
BBa_K1022125	.	ribosome_entry_site	1331	1342	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J13002
BBa_K1022125	.	conserved_region	1335	1338	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1022125	.	promoter	1269	1322	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_J13002
BBa_K1022125	.	engineered_region	1269	1322	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K1022125	.	non_covalent_binding_site	1294	1312	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K1022125	.	non_covalent_binding_site	1269	1287	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K1022125	.	promoter	1288	1293	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K1022125	.	promoter	1311	1316	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K1022125	.	CDS	1349	2123	.	+	0	ID=BBa_C0051;Name=BBa_C0051;Parent=BBa_K1022108
BBa_K1022125	.	engineered_tag	2099	2123	.	+	0	ID=annotation2213991;Name=Help:Barcodes;Parent=BBa_C0051
BBa_K1022125	.	CDS	1352	2059	.	+	0	ID=annotation23334;Name=cI lambda;Parent=BBa_C0051
BBa_K1022125	.	tag	2060	2092	.	+	0	ID=annotation23335;Name=LVA;Parent=BBa_C0051
BBa_K1022125	.	engineered_region	3092	4876	.	+	0	ID=BBa_K1022109;Name=BBa_K1022109;Parent=BBa_K1022123
>BBa_K1022125
taatacgactcactatagggagagaaagaggagaaatactagatggctagcatgcatcatcatcatcatcattcggact
cagaagtcaatcaagaagctaagccagaggtcaagccagaagtcaagcctgagactcacatcaatttaaaggtgtccga
tggatcttcagagatcttcttcaagatcaaaaagaccactcctttaagaaggctgatggaagcgttcgctaaacgtcag
ggtaaggaaatggactccttaagattcttgtacgacggtattaggattcaagctgatcagacccctgaagatttggaca
tggatgataacgatattattgaggctcacagagaacagaccggtggtatcatcggtcacctgatcaaaaccgcgctggg
tatgctgggtctgtaattactagagaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagc
gcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagc
ctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatac
tcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttacta
agtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattag
cctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttg
cgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattatta
cgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggat
tagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgata
gtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgtttta
tctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatact
agagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaat
actagatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaa
aaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgcttta
tttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaattta
gcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagta
ccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgg
gtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggct
ccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagc
cagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaaccca
cagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgt
ttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagcc
aggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctct
actagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtaacaccgtgcgtgttgactatt
ttacctctggcggtgataatggttgcttaccggaaagaggagaaatactagatggctagccttgttcctgaattaaatg
aaaaagacgatgaccaagtacaaaaagctttggcatcgagagaaaatactcagttaatgaatagagataatatagagat
aacagtacgtgattttaagaccttggcaccacgaagatggctaaatgacactatcattgagttttttatgaaatacatt
gaaaaatctacccctaatacagtggcgtttaattcatttttctataccaatttatcagaaaggggttatcaaggcgtcc
ggaggtggatgaagagaaagaagacacaaattgataaacttgataaaatctttacaccaataaatttgaaccaatccca
ctgggcgttgggcataattgatttaaaaaagaaaactataggttacgtagattcattatcgaatggtccaaatgctatg
agtttcgctatactgactgacttgcaaaaatatgttatggaggaaagtaagcatacaataggagaagactttgatttga
ttcatttagattgtccgcagcaaccaaatggctacgactgtggaatatatgtttgtatgaatactctctatggaagtgc
agatgcgccattggattttgattataaagatgcgattaggatgagaagatttattgcccatttgattttaaccgacgct
ttaaaacgggatccgaactcgagctccgtcgacaagcttgcggccgcactcgagcaccaccaccaccaccactgagtac
attactagagcttaaaaggagggtctatggcagcacctagaatatcattttcgccctctgatattctatttggtgttct
cgatcgcttgttcaaagataacgctaccgggaaggttcttgcttcccgggtagctgtcgtaattcttttgtttataatg
gcgattgtttggtataggggagatagtttctttgagtactataagcaatcaaagtatgaaacatacagtgaaattattg
aaaaggaaagaactgcacgctttgaatctgtcgccctggaacaactccagatagttcatatatcatctgaggcagactt
tagtgcggtgtattctttccgccctaaaaacttaaactattttgttgatattatagcatacgaaggaaaattaccttca
acaataagtgaaaaatcacttggaggatatcctgttgataaaactatggatgaatatacagttcatttaaatggacgtc
attattattccaactcaaaatttgcttttttaccaactaaaaagcctactcccgaaataaactacatgtacagttgtcc
atattttaatttggataatatctatgctggaacgataaccatgtactggtatagaaatgatcatataagtaatgaccgc
cttgaatcaatatgtgctcaggcggccagaatattaggaagggctaaataattatactagagatacttaggaggtatta
tgaatatatttgaaatgttacgtatagatgaaggtcttagacttaaaatctataaagacacagaaggctattacactat
tggcatcggtcatttgcttacaaaaagtccatcacttaatgctgctaaatctgaattagataaagctattgggcgtaat
tgcaatggtgtaattacaaaagatgaggctgaaaaactctttaatcaggatgttgatgctgctgttcgcggaatcctga
gaaatgctaaattaaaaccggtttatgattctcttgatgcggttcgtcgctgtgcattgattaatatggttttccaaat
gggagaaaccggtgtggcaggatttactaactctttacgtatgcttcaacaaaaacgctgggatgaagcagcagttaac
ttagctaaaagtagatggtataatcaaacacctaatcgcgcaaaacgagtcattacaacgtttagaactggcacttggg
acgcgtataaaaatctataaagctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttc
gttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgttta
tatactagagttgacagctagctcagtcctagggactatgctagctactagaggataggaggcctttatggccttaaaa
gcaacagcactttttgccatgctaggattgtcatttgttttatctccatcgattgaagcgaatgtcgatcctcattttg
ataaatttatggaatctggtattaggcacgtttatatgctttttgaaaataaaagcgtagaatcgtctgaacaattcta
tagttttatgagaacgacctataaaaatgacccgtgctcttctgattttgaatgtatagagcgaggcgcggagatggca
caatcatacgctagaattatgaacattaaattggagactgaatgaaattactagagccaggcatcaaataaaacgaaag
gctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc