BBa_K1014014
type: ComponentDefinition
persistent identity: BBa K1014014
display id: BBa_K1014014
version: 1
was generated by: iGEM to SBOL conversion
description: hrpL AND Gate device
created: 2013-09-01T11:00:00Z
modified: 2015-05-08T01:08:42Z
mutable provenance: E.coli
owned by: james
owned by: myers
mutable description: hrpL AND Gate device
discontinued: false
dominant: false
group u list: _999_1320_
m user id: 0
owner id: 11931
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Jcat
star: false
creator: Xiangmiao Zeng
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_K1014011
component: BBa_K1014005
component: BBa_K1014003
sequence annotation: BBa_K1014003
sequence annotation: BBa_K1014005
sequence annotation: BBa_K1014011
sequence: https://synbiohub.org/public/igem/BBa_K1014014_sequence/1

BBa_K1014011
type: Component
persistent identity: component2334427
display id: component2334427
version: 1
top level: BBa_K1014014
definition: BBa_K1014011
access: public

BBa_K1014005
type: Component
persistent identity: component2334413
display id: component2334413
version: 1
top level: BBa_K1014014
definition: BBa_K1014005
access: public

BBa_K1014003
type: Component
persistent identity: component2334397
display id: component2334397
version: 1
top level: BBa_K1014014
definition: BBa_K1014003
access: public

BBa_K1014003
type: SequenceAnnotation
persistent identity: annotation2334397
display id: annotation2334397
version: 1
top level: BBa_K1014014
location: https://synbiohub.org/public/igem/BBa_K1014014/annotation2334397/range2334397/1
component: BBa_K1014003

https://synbiohub.org/public/igem/BBa_K1014014/annotation2334397/range2334397/1
type: Range
persistent identity: range2334397
display id: range2334397
version: 1
top level: BBa_K1014014
direction: direction/reverse
start: 1
end: 1169
orientation: inline

BBa_K1014005
type: SequenceAnnotation
persistent identity: annotation2334413
display id: annotation2334413
version: 1
top level: BBa_K1014014
location: https://synbiohub.org/public/igem/BBa_K1014014/annotation2334413/range2334413/1
component: BBa_K1014005

https://synbiohub.org/public/igem/BBa_K1014014/annotation2334413/range2334413/1
type: Range
persistent identity: range2334413
display id: range2334413
version: 1
top level: BBa_K1014014
direction: direction/reverse
start: 1178
end: 2384
orientation: inline

BBa_K1014011
type: SequenceAnnotation
persistent identity: annotation2334427
display id: annotation2334427
version: 1
top level: BBa_K1014014
location: https://synbiohub.org/public/igem/BBa_K1014014/annotation2334427/range2334427/1
component: BBa_K1014011

https://synbiohub.org/public/igem/BBa_K1014014/annotation2334427/range2334427/1
type: Range
persistent identity: range2334427
display id: range2334427
version: 1
top level: BBa_K1014014
direction: direction/reverse
start: 2393
end: 3474
orientation: inline

BBa_K1014003
type: ComponentDefinition
persistent identity: BBa K1014003
display id: BBa_K1014003
version: 1
was generated by: iGEM to SBOL conversion
description: HrpR Protein Generator(S)
created: 2013-08-28T11:00:00Z
modified: 2015-05-08T01:08:42Z
mutable provenance: E.coli
owned by: james
owned by: myers
mutable description: This device will produce HrpR protein treated with IPTG under strong RBS.
discontinued: false
dominant: false
experience: experience/None
group u list: _999_1320_
m user id: 0
owner id: 11931
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: JCAT
star: false
creator: Xiangmiao Zeng
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_B0012
component: BBa_B0010
component: BBa_K1014001
component: BBa_B0030
component: BBa_R0011
sequence annotation: BBa_R0011
sequence annotation: BBa_B0030
sequence annotation: BBa_K1014001
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K1014003_sequence/1

BBa_B0012
type: Component
persistent identity: component2333378
display id: component2333378
version: 1
top level: BBa_K1014003
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component2333376
display id: component2333376
version: 1
top level: BBa_K1014003
definition: BBa_B0010
access: public

BBa_K1014001
type: Component
persistent identity: component2333375
display id: component2333375
version: 1
top level: BBa_K1014003
definition: BBa_K1014001
access: public

BBa_B0030
type: Component
persistent identity: component2333373
display id: component2333373
version: 1
top level: BBa_K1014003
definition: BBa_B0030
access: public

BBa_R0011
type: Component
persistent identity: component2333367
display id: component2333367
version: 1
top level: BBa_K1014003
definition: lacI+pL
access: public

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation2333367
display id: annotation2333367
version: 1
top level: BBa_K1014003
location: https://synbiohub.org/public/igem/BBa_K1014003/annotation2333367/range2333367/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_K1014003/annotation2333367/range2333367/1
type: Range
persistent identity: range2333367
display id: range2333367
version: 1
top level: BBa_K1014003
direction: direction/forward
start: 1
end: 54
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2333373
display id: annotation2333373
version: 1
top level: BBa_K1014003
location: https://synbiohub.org/public/igem/BBa_K1014003/annotation2333373/range2333373/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K1014003/annotation2333373/range2333373/1
type: Range
persistent identity: range2333373
display id: range2333373
version: 1
top level: BBa_K1014003
direction: direction/forward
start: 64
end: 78
orientation: inline

BBa_K1014001
type: SequenceAnnotation
persistent identity: annotation2333375
display id: annotation2333375
version: 1
top level: BBa_K1014003
location: https://synbiohub.org/public/igem/BBa_K1014003/annotation2333375/range2333375/1
component: BBa_K1014001

https://synbiohub.org/public/igem/BBa_K1014003/annotation2333375/range2333375/1
type: Range
persistent identity: range2333375
display id: range2333375
version: 1
top level: BBa_K1014003
direction: direction/reverse
start: 85
end: 1032
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2333376
display id: annotation2333376
version: 1
top level: BBa_K1014003
location: https://synbiohub.org/public/igem/BBa_K1014003/annotation2333376/range2333376/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K1014003/annotation2333376/range2333376/1
type: Range
persistent identity: range2333376
display id: range2333376
version: 1
top level: BBa_K1014003
direction: direction/forward
start: 1041
end: 1120
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2333378
display id: annotation2333378
version: 1
top level: BBa_K1014003
location: https://synbiohub.org/public/igem/BBa_K1014003/annotation2333378/range2333378/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K1014003/annotation2333378/range2333378/1
type: Range
persistent identity: range2333378
display id: range2333378
version: 1
top level: BBa_K1014003
direction: direction/forward
start: 1129
end: 1169
orientation: inline

BBa_K1014011
type: ComponentDefinition
persistent identity: BBa K1014011
display id: BBa_K1014011
version: 1
was generated by: iGEM to SBOL conversion
description: RFP Protein Generator(S)
created: 2013-08-28T11:00:00Z
modified: 2015-05-08T01:08:42Z
mutable provenance: E.coli
owned by: james
owned by: myers
mutable description: This device will produce RFP protein when hrpL promoter is on under medium RBS.
discontinued: false
dominant: false
experience: experience/None
group u list: _999_1320_
m user id: 0
owner id: 11931
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: JCAT
star: false
creator: Xiangmiao Zeng
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0010
component: BBa_E1010
component: BBa_B0012
component: BBa_B0030
component: BBa_K1014002
sequence annotation: BBa_K1014002
sequence annotation: BBa_B0030
sequence annotation: BBa_E1010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K1014011_sequence/1

BBa_B0010
type: Component
persistent identity: component2333521
display id: component2333521
version: 1
top level: BBa_K1014011
definition: BBa_B0010
access: public

BBa_E1010
type: Component
persistent identity: component2333520
display id: component2333520
version: 1
top level: BBa_K1014011
definition: mRFP1
access: public

BBa_B0012
type: Component
persistent identity: component2333523
display id: component2333523
version: 1
top level: BBa_K1014011
definition: BBa_B0012
access: public

BBa_B0030
type: Component
persistent identity: component2333516
display id: component2333516
version: 1
top level: BBa_K1014011
definition: BBa_B0030
access: public

BBa_K1014002
type: Component
persistent identity: component2333514
display id: component2333514
version: 1
top level: BBa_K1014011
definition: BBa_K1014002
access: public

BBa_K1014002
type: SequenceAnnotation
persistent identity: annotation2333514
display id: annotation2333514
version: 1
top level: BBa_K1014011
location: https://synbiohub.org/public/igem/BBa_K1014011/annotation2333514/range2333514/1
component: BBa_K1014002

https://synbiohub.org/public/igem/BBa_K1014011/annotation2333514/range2333514/1
type: Range
persistent identity: range2333514
display id: range2333514
version: 1
top level: BBa_K1014011
direction: direction/reverse
start: 1
end: 210
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2333516
display id: annotation2333516
version: 1
top level: BBa_K1014011
location: https://synbiohub.org/public/igem/BBa_K1014011/annotation2333516/range2333516/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K1014011/annotation2333516/range2333516/1
type: Range
persistent identity: range2333516
display id: range2333516
version: 1
top level: BBa_K1014011
direction: direction/forward
start: 219
end: 233
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2333520
display id: annotation2333520
version: 1
top level: BBa_K1014011
location: https://synbiohub.org/public/igem/BBa_K1014011/annotation2333520/range2333520/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_K1014011/annotation2333520/range2333520/1
type: Range
persistent identity: range2333520
display id: range2333520
version: 1
top level: BBa_K1014011
direction: direction/reverse
start: 240
end: 945
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2333521
display id: annotation2333521
version: 1
top level: BBa_K1014011
location: https://synbiohub.org/public/igem/BBa_K1014011/annotation2333521/range2333521/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K1014011/annotation2333521/range2333521/1
type: Range
persistent identity: range2333521
display id: range2333521
version: 1
top level: BBa_K1014011
direction: direction/forward
start: 954
end: 1033
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2333523
display id: annotation2333523
version: 1
top level: BBa_K1014011
location: https://synbiohub.org/public/igem/BBa_K1014011/annotation2333523/range2333523/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K1014011/annotation2333523/range2333523/1
type: Range
persistent identity: range2333523
display id: range2333523
version: 1
top level: BBa_K1014011
direction: direction/forward
start: 1042
end: 1082
orientation: inline

BBa_K1014005
type: ComponentDefinition
persistent identity: BBa K1014005
display id: BBa_K1014005
version: 1
was generated by: iGEM to SBOL conversion
description: HrpS Protein Generator(S)
created: 2013-08-31T11:00:00Z
modified: 2015-05-08T01:08:42Z
mutable provenance: E.coli
owned by: james
owned by: myers
mutable description: This device will produce HrpS protein constitutively under strong RBS and is repressed by TetR.
discontinued: false
dominant: false
group u list: _999_1320_
m user id: 0
owner id: 11931
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: JCAT
star: false
creator: Xiangmiao Zeng
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_R0040
component: BBa_K1014000
component: BBa_B0030
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_R0040
sequence annotation: BBa_B0030
sequence annotation: BBa_K1014000
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K1014005_sequence/1

BBa_R0040
type: Component
persistent identity: component2334296
display id: component2334296
version: 1
top level: BBa_K1014005
definition: p(tetR)
access: public

BBa_K1014000
type: Component
persistent identity: component2334304
display id: component2334304
version: 1
top level: BBa_K1014005
definition: BBa_K1014000
access: public

BBa_B0030
type: Component
persistent identity: component2334302
display id: component2334302
version: 1
top level: BBa_K1014005
definition: BBa_B0030
access: public

BBa_B0012
type: Component
persistent identity: component2334307
display id: component2334307
version: 1
top level: BBa_K1014005
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component2334305
display id: component2334305
version: 1
top level: BBa_K1014005
definition: BBa_B0010
access: public

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2334296
display id: annotation2334296
version: 1
top level: BBa_K1014005
location: https://synbiohub.org/public/igem/BBa_K1014005/annotation2334296/range2334296/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K1014005/annotation2334296/range2334296/1
type: Range
persistent identity: range2334296
display id: range2334296
version: 1
top level: BBa_K1014005
direction: direction/forward
start: 1
end: 54
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2334302
display id: annotation2334302
version: 1
top level: BBa_K1014005
location: https://synbiohub.org/public/igem/BBa_K1014005/annotation2334302/range2334302/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K1014005/annotation2334302/range2334302/1
type: Range
persistent identity: range2334302
display id: range2334302
version: 1
top level: BBa_K1014005
direction: direction/forward
start: 63
end: 77
orientation: inline

BBa_K1014000
type: SequenceAnnotation
persistent identity: annotation2334304
display id: annotation2334304
version: 1
top level: BBa_K1014005
location: https://synbiohub.org/public/igem/BBa_K1014005/annotation2334304/range2334304/1
component: BBa_K1014000

https://synbiohub.org/public/igem/BBa_K1014005/annotation2334304/range2334304/1
type: Range
persistent identity: range2334304
display id: range2334304
version: 1
top level: BBa_K1014005
direction: direction/reverse
start: 84
end: 1070
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2334305
display id: annotation2334305
version: 1
top level: BBa_K1014005
location: https://synbiohub.org/public/igem/BBa_K1014005/annotation2334305/range2334305/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K1014005/annotation2334305/range2334305/1
type: Range
persistent identity: range2334305
display id: range2334305
version: 1
top level: BBa_K1014005
direction: direction/forward
start: 1079
end: 1158
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2334307
display id: annotation2334307
version: 1
top level: BBa_K1014005
location: https://synbiohub.org/public/igem/BBa_K1014005/annotation2334307/range2334307/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K1014005/annotation2334307/range2334307/1
type: Range
persistent identity: range2334307
display id: range2334307
version: 1
top level: BBa_K1014005
direction: direction/forward
start: 1167
end: 1207
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

mRFP1
type: ComponentDefinition
persistent identity: BBa E1010
display id: BBa_E1010
version: 1
was generated by: iGEM to SBOL conversion
description: **highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)
created: 2004-07-27T11:00:00Z
modified: 2015-08-31T04:07:26Z
mutable provenance: Campbell et al., PNAS v99 p7877 URL
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: monomeric RFP: Red Fluorescent Protein. Excitation peak: 584 nm Emission peak: 607 nm
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 52
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: TAATAA double stop codon added (DE). Four silent mutations made to remove three EcoRI sites and one PstI site: A28G, A76G, A349G, G337A.
star: true
creator: Drew Endy
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: mrfp1
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_E1010_sequence/1

mrfp1
type: SequenceAnnotation
persistent identity: annotation1014044
display id: annotation1014044
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
type: Range
persistent identity: range1014044
display id: range1014044
version: 1
top level: mRFP1
direction: direction/forward
start: 1
end: 675
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214014
display id: annotation2214014
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
type: Range
persistent identity: range2214014
display id: range2214014
version: 1
top level: mRFP1
direction: direction/forward
start: 682
end: 706
orientation: inline

p(tetR)
type: ComponentDefinition
persistent identity: BBa R0040
display id: BBa_R0040
version: 1
was generated by: iGEM to SBOL conversion
description: TetR repressible promoter
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: Lutz, R., Bujard, H., Nucleic Acids Research (1997) 25, 1203-1210.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Sequence for pTet inverting regulator driven by the TetR protein.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0040
sequence annotation: TetR 1
sequence annotation: -35
sequence annotation: TetR 2
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0040_sequence/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1986784
display id: annotation1986784
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
type: Range
persistent identity: range1986784
display id: range1986784
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 54
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1986783
display id: annotation1986783
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
type: Range
persistent identity: range1986783
display id: range1986783
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1986785
display id: annotation1986785
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
type: Range
persistent identity: range1986785
display id: range1986785
version: 1
top level: p(tetR)
direction: direction/forward
start: 20
end: 25
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation1986786
display id: annotation1986786
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
type: Range
persistent identity: range1986786
display id: range1986786
version: 1
top level: p(tetR)
direction: direction/forward
start: 26
end: 44
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986787
display id: annotation1986787
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
type: Range
persistent identity: range1986787
display id: range1986787
version: 1
top level: p(tetR)
direction: direction/forward
start: 43
end: 48
orientation: inline

BBa_B0030
type: ComponentDefinition
persistent identity: BBa B0030
display id: BBa_B0030
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.1 (strong) -- modified from R. Weiss
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _44_46_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS-1\Strong
sequence annotation: BBa_B0030
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0030_sequence/1

RBS-1\Strong
type: SequenceAnnotation
persistent identity: annotation1701
display id: annotation1701
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
type: Range
persistent identity: range1701
display id: range1701
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation7025
display id: annotation7025
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
type: Range
persistent identity: range7025
display id: range7025
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1702
display id: annotation1702
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
type: Range
persistent identity: range1702
display id: range1702
version: 1
top level: BBa_B0030
direction: direction/forward
start: 8
end: 12
orientation: inline

lacI+pL
type: ComponentDefinition
persistent identity: BBa R0011
display id: BBa_R0011
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (lacI regulated, lambda pL hybrid)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: represillator of Elowitz and Leibler (2000)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong promotion that can nevertheless be tightly repressed by LacI, the Lac inhibitor (i.e. repressor) (<bb_part>BBa_C0010</bb_part>) ([LUTZ97]). The activity of the promoter can be regulated over a >600-fold range by IPTG in E.Coli DH5-alpha-Z1 (same paper reference).
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: hybrid promoter design to create strong promoter that is, at the same time, highly repressible. note that the upstream operator installed in this hybrid is slightly different than the one in the original source (Lutz and Bujard, 1997). the most upstream operator region is slightly truncated in the represillator version, so that both operators in the hybrid are the same sequence. see references for details. also, the sequence has been truncated after the transcriptional start site. LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and increase transcription. This part is incompatible with environments containing lactose or lactose analogs.
star: true
creator: Neelaksh Varshney, Grace Kenney, Daniel Shen, Samantha Sutton
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0011
sequence annotation: lac O1
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0011_sequence/1

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation7064
display id: annotation7064
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation7064/range7064/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0011/annotation7064/range7064/1
type: Range
persistent identity: range7064
display id: range7064
version: 1
top level: lacI+pL
direction: direction/forward
start: 1
end: 54
orientation: inline

lac O1
type: SequenceAnnotation
persistent identity: annotation1999
display id: annotation1999
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation1999/range1999/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_R0011/annotation1999/range1999/1
type: Range
persistent identity: range1999
display id: range1999
version: 1
top level: lacI+pL
direction: direction/forward
start: 3
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2000
display id: annotation2000
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2000/range2000/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0011/annotation2000/range2000/1
type: Range
persistent identity: range2000
display id: range2000
version: 1
top level: lacI+pL
direction: direction/forward
start: 20
end: 25
orientation: inline

lac O1
type: SequenceAnnotation
persistent identity: annotation2001
display id: annotation2001
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2001/range2001/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_R0011/annotation2001/range2001/1
type: Range
persistent identity: range2001
display id: range2001
version: 1
top level: lacI+pL
direction: direction/forward
start: 26
end: 42
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2002
display id: annotation2002
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2002/range2002/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0011/annotation2002/range2002/1
type: Range
persistent identity: range2002
display id: range2002
version: 1
top level: lacI+pL
direction: direction/forward
start: 43
end: 48
orientation: inline

BBa_K1014001
type: ComponentDefinition
persistent identity: BBa K1014001
display id: BBa_K1014001
version: 1
was generated by: iGEM to SBOL conversion
description: HrpR Gene
created: 2013-08-26T11:00:00Z
modified: 2015-05-08T01:08:42Z
mutable provenance: E.coli
owned by: james
owned by: myers
mutable description: This codes for HrpR protein which is needed for hrpL AND Gate
discontinued: false
dominant: false
group u list: _999_1320_
m user id: 0
owner id: 11931
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: ATGTCTACCGACATCGACAACGACGTTCTGACCTGCTGCAACGTTACCGCTCTGTCTGCTGGTCACCAGATCGTTATGAACTCTGTTCTGATGGACATGGACCTGCTGCTGTGCGGTGAAACCGGTACCGGTAAAGACACCCTGGCTTCTCGTATCCACGAACTGTCTTCTCGTACCGGTCCGTTCGTTGGTATGAACTGCGCTGCTATCCCGGAATCTCTGGCTGAATCTCAGCTGTTCGGTGTTGTTAACGGTGCTTTTACTGGTGTCTGCCGTGCTCGTGAAGGTTACATCGAAGCTTCTTCTGGTGGTACCCTGTACCTGGACGAAATCGACTCTATGCCGCTGTCTCTGCAAGCTAAACTGCTGCGTGTTCTGGAATCTCGTGGTGTTGAACGTCTGGGTTCTACCGACTTCATCCCGCTGGACCTGCGTGTTATCGCTTCTGCTCAGCGTCCGCTGGACGAACTGGTTGAACAGGGTCTGTTCCGTCGTGACCTGTTCTTCCGTCTGAACGTTCTGACCCTGCAACTGCCGGCTCTGCGTAAACGTCGTGAACAGATCCTGCCGCTGTTCGACCAGTTCACCCAGGACGTTGCTGCTGAATCTGGTCGTTCTGTTCCGACCCTGGACAACCGTCGTGTTCAGATCCTGCTGTCTCACGACTGGCCGGGTAACGTTCGTGAACTGAAATCTGCTGCTAAACGTTTCGTTCTGGGTCTGCCGCTGCTGGGTGCTGAACCGGTTGAAGCTCGTGACCCGGTTACCGGTCTGCGTATGCAGATGCGTGTTATCGAAAAAATGCTGATCCAGGACGCTCTGAAACGTCACCGTCACAACTTCGACGCTGTTCTGGAAGAACTGGAACTGCCGCGTCGTACCCTGTACCACCGTATGAAAGAACTGGGTGTTGCTTCTCACATCGACCTGGTTGCTGAATCTTAATAA
star: false
creator: Xiangmiao Zeng
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1014001_sequence/1

BBa_K1014000
type: ComponentDefinition
persistent identity: BBa K1014000
display id: BBa_K1014000
version: 1
was generated by: iGEM to SBOL conversion
description: HrpS Gene
created: 2013-08-27T11:00:00Z
modified: 2015-05-08T01:08:42Z
mutable provenance: E.coli
owned by: james
owned by: myers
mutable description: This codes for HrpS protein which is needed for hrpL AND Gate
discontinued: false
dominant: false
experience: experience/None
group u list: _1320_
m user id: 0
owner id: 11931
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: JCAT
star: false
creator: Xiangmiao Zeng
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1014000_sequence/1

BBa_K1014002
type: ComponentDefinition
persistent identity: BBa K1014002
display id: BBa_K1014002
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter hrpL
created: 2013-08-27T11:00:00Z
modified: 2015-05-08T01:08:42Z
mutable provenance: E.coli
owned by: james
owned by: myers
mutable description: This promoter will be active,when both HrpS and HrpR are present.
discontinued: false
dominant: false
group u list: _999_1320_
m user id: 0
owner id: 11931
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: n/a
star: false
creator: Xiangmiao Zeng
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K1014002_sequence/1

https://synbiohub.org/public/igem/BBa_K1014014_sequence/1
type: Sequence
persistent identity: BBa K1014014 sequence
display id: BBa_K1014014_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagattaaagaggagaaatactagatgtctaccgacatcgacaacgacgttctgacctgctgcaacgttaccgctctgtctgctggtcaccagatcgttatgaactctgttctgatggacatggacctgctgctgtgcggtgaaaccggtaccggtaaagacaccctggcttctcgtatccacgaactgtcttctcgtaccggtccgttcgttggtatgaactgcgctgctatcccggaatctctggctgaatctcagctgttcggtgttgttaacggtgcttttactggtgtctgccgtgctcgtgaaggttacatcgaagcttcttctggtggtaccctgtacctggacgaaatcgactctatgccgctgtctctgcaagctaaactgctgcgtgttctggaatctcgtggtgttgaacgtctgggttctaccgacttcatcccgctggacctgcgtgttatcgcttctgctcagcgtccgctggacgaactggttgaacagggtctgttccgtcgtgacctgttcttccgtctgaacgttctgaccctgcaactgccggctctgcgtaaacgtcgtgaacagatcctgccgctgttcgaccagttcacccaggacgttgctgctgaatctggtcgttctgttccgaccctggacaaccgtcgtgttcagatcctgctgtctcacgactggccgggtaacgttcgtgaactgaaatctgctgctaaacgtttcgttctgggtctgccgctgctgggtgctgaaccggttgaagctcgtgacccggttaccggtctgcgtatgcagatgcgtgttatcgaaaaaatgctgatccaggacgctctgaaacgtcaccgtcacaacttcgacgctgttctggaagaactggaactgccgcgtcgtaccctgtaccaccgtatgaaagaactgggtgttgcttctcacatcgacctggttgctgaatcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagattaaagaggagaaatactagatgaccatccgtaactctaacgaatctgttcagtctctgcactgcgcttctcacgaatcttctctgacccacccgtctgaagacatccacggttctctgtcttctctgatccacaccatcgctccgctgaacgttgacatcgttctggaaggtgaaaccggtaccggtaaagacaccctggctcgtcgtatccaccagctgtctggttgcgctggtccgctggttgctgttaactgcgctgctgttccggaaaccctggctgaatctgaactgttcggtgttgtttctggtgcttacaccggtgcttctcgttctcgtgctggttacctggaaaccgctgacaaaggtatcctgttcctggacgaaatcgactctatgccgctgaccctgcaagctaaaatgctgcgtgttctggaatctcgtggtatcgaacgtctgggttctacccagttcaaaccggttgacatgcgtgttatcgttgctacccagaccccgctgcaaaaactggttgacgaaggtcgtttccgtcgtgacctgtacttccgtctggacaccgttaaaatccagctgccgaccctgcgttctcgtaccgaaatcatcctgccgctgttcgaacgtttcctgctggaagcttctgctcgtctgaaacgtccggctccgggtctgtctgctatcatccaggaacagctgctgatgcacggttggccgggtaacatccgtgaactgaaagctgctgctga…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1014003_sequence/1
type: Sequence
persistent identity: BBa K1014003 sequence
display id: BBa_K1014003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagattaaagaggagaaatactagatgtctaccgacatcgacaacgacgttctgacctgctgcaacgttaccgctctgtctgctggtcaccagatcgttatgaactctgttctgatggacatggacctgctgctgtgcggtgaaaccggtaccggtaaagacaccctggcttctcgtatccacgaactgtcttctcgtaccggtccgttcgttggtatgaactgcgctgctatcccggaatctctggctgaatctcagctgttcggtgttgttaacggtgcttttactggtgtctgccgtgctcgtgaaggttacatcgaagcttcttctggtggtaccctgtacctggacgaaatcgactctatgccgctgtctctgcaagctaaactgctgcgtgttctggaatctcgtggtgttgaacgtctgggttctaccgacttcatcccgctggacctgcgtgttatcgcttctgctcagcgtccgctggacgaactggttgaacagggtctgttccgtcgtgacctgttcttccgtctgaacgttctgaccctgcaactgccggctctgcgtaaacgtcgtgaacagatcctgccgctgttcgaccagttcacccaggacgttgctgctgaatctggtcgttctgttccgaccctggacaaccgtcgtgttcagatcctgctgtctcacgactggccgggtaacgttcgtgaactgaaatctgctgctaaacgtttcgttctgggtctgccgctgctgggtgctgaaccggttgaagctcgtgacccggttaccggtctgcgtatgcagatgcgtgttatcgaaaaaatgctgatccaggacgctctgaaacgtcaccgtcacaacttcgacgctgttctggaagaactggaactgccgcgtcgtaccctgtaccaccgtatgaaagaactgggtgttgcttctcacatcgacctggttgctgaatcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1014011_sequence/1
type: Sequence
persistent identity: BBa K1014011 sequence
display id: BBa_K1014011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gccggattatgtccgctgagtgggtcacggtcccggatcagttcccttgcgaagctgaccgatgtttttgatgccaaaagctgttgtggcaaaaaacggtttgcgcaaagttttgtattacaaagaatttcacattttaaatatatctttataaatcaatcagttatttctatttttaagctggcatggttatcgctatagggcttgtactactagagattaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1014005_sequence/1
type: Sequence
persistent identity: BBa K1014005 sequence
display id: BBa_K1014005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagattaaagaggagaaatactagatgaccatccgtaactctaacgaatctgttcagtctctgcactgcgcttctcacgaatcttctctgacccacccgtctgaagacatccacggttctctgtcttctctgatccacaccatcgctccgctgaacgttgacatcgttctggaaggtgaaaccggtaccggtaaagacaccctggctcgtcgtatccaccagctgtctggttgcgctggtccgctggttgctgttaactgcgctgctgttccggaaaccctggctgaatctgaactgttcggtgttgtttctggtgcttacaccggtgcttctcgttctcgtgctggttacctggaaaccgctgacaaaggtatcctgttcctggacgaaatcgactctatgccgctgaccctgcaagctaaaatgctgcgtgttctggaatctcgtggtatcgaacgtctgggttctacccagttcaaaccggttgacatgcgtgttatcgttgctacccagaccccgctgcaaaaactggttgacgaaggtcgtttccgtcgtgacctgtacttccgtctggacaccgttaaaatccagctgccgaccctgcgttctcgtaccgaaatcatcctgccgctgttcgaacgtttcctgctggaagcttctgctcgtctgaaacgtccggctccgggtctgtctgctatcatccaggaacagctgctgatgcacggttggccgggtaacatccgtgaactgaaagctgctgctgaacgttgggttctgggtctgtctccggttccgatcgcttctgacggtgacgttcaggttatgggtgaagacaccccgctgacctctctgaaagttcgtctgcgtcgtatagagaggttcctgatccaggaggctctgcaacgtaacgaccactgcatcgacaccgttgttaacgaactgggtatcccgaaacgtaccctgtaccaccgtatcaaactgctgaacgttgctgctcgtggtctgtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E1010_sequence/1
type: Sequence
persistent identity: BBa E1010 sequence
display id: BBa_E1010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0040_sequence/1
type: Sequence
persistent identity: BBa R0040 sequence
display id: BBa_R0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0030_sequence/1
type: Sequence
persistent identity: BBa B0030 sequence
display id: BBa_B0030_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0011_sequence/1
type: Sequence
persistent identity: BBa R0011 sequence
display id: BBa_R0011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1014001_sequence/1
type: Sequence
persistent identity: BBa K1014001 sequence
display id: BBa_K1014001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtctaccgacatcgacaacgacgttctgacctgctgcaacgttaccgctctgtctgctggtcaccagatcgttatgaactctgttctgatggacatggacctgctgctgtgcggtgaaaccggtaccggtaaagacaccctggcttctcgtatccacgaactgtcttctcgtaccggtccgttcgttggtatgaactgcgctgctatcccggaatctctggctgaatctcagctgttcggtgttgttaacggtgcttttactggtgtctgccgtgctcgtgaaggttacatcgaagcttcttctggtggtaccctgtacctggacgaaatcgactctatgccgctgtctctgcaagctaaactgctgcgtgttctggaatctcgtggtgttgaacgtctgggttctaccgacttcatcccgctggacctgcgtgttatcgcttctgctcagcgtccgctggacgaactggttgaacagggtctgttccgtcgtgacctgttcttccgtctgaacgttctgaccctgcaactgccggctctgcgtaaacgtcgtgaacagatcctgccgctgttcgaccagttcacccaggacgttgctgctgaatctggtcgttctgttccgaccctggacaaccgtcgtgttcagatcctgctgtctcacgactggccgggtaacgttcgtgaactgaaatctgctgctaaacgtttcgttctgggtctgccgctgctgggtgctgaaccggttgaagctcgtgacccggttaccggtctgcgtatgcagatgcgtgttatcgaaaaaatgctgatccaggacgctctgaaacgtcaccgtcacaacttcgacgctgttctggaagaactggaactgccgcgtcgtaccctgtaccaccgtatgaaagaactgggtgttgcttctcacatcgacctggttgctgaatcttaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1014000_sequence/1
type: Sequence
persistent identity: BBa K1014000 sequence
display id: BBa_K1014000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaccatccgtaactctaacgaatctgttcagtctctgcactgcgcttctcacgaatcttctctgacccacccgtctgaagacatccacggttctctgtcttctctgatccacaccatcgctccgctgaacgttgacatcgttctggaaggtgaaaccggtaccggtaaagacaccctggctcgtcgtatccaccagctgtctggttgcgctggtccgctggttgctgttaactgcgctgctgttccggaaaccctggctgaatctgaactgttcggtgttgtttctggtgcttacaccggtgcttctcgttctcgtgctggttacctggaaaccgctgacaaaggtatcctgttcctggacgaaatcgactctatgccgctgaccctgcaagctaaaatgctgcgtgttctggaatctcgtggtatcgaacgtctgggttctacccagttcaaaccggttgacatgcgtgttatcgttgctacccagaccccgctgcaaaaactggttgacgaaggtcgtttccgtcgtgacctgtacttccgtctggacaccgttaaaatccagctgccgaccctgcgttctcgtaccgaaatcatcctgccgctgttcgaacgtttcctgctggaagcttctgctcgtctgaaacgtccggctccgggtctgtctgctatcatccaggaacagctgctgatgcacggttggccgggtaacatccgtgaactgaaagctgctgctgaacgttgggttctgggtctgtctccggttccgatcgcttctgacggtgacgttcaggttatgggtgaagacaccccgctgacctctctgaaagttcgtctgcgtcgtatagagaggttcctgatccaggaggctctgcaacgtaacgaccactgcatcgacaccgttgttaacgaactgggtatcccgaaacgtaccctgtaccaccgtatcaaactgctgaacgttgctgctcgtggtctgtaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1014002_sequence/1
type: Sequence
persistent identity: BBa K1014002 sequence
display id: BBa_K1014002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gccggattatgtccgctgagtgggtcacggtcccggatcagttcccttgcgaagctgaccgatgtttttgatgccaaaagctgttgtggcaaaaaacggtttgcgcaaagttttgtattacaaagaatttcacattttaaatatatctttataaatcaatcagttatttctatttttaagctggcatggttatcgctatagggcttgtac
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z