BBa_K091193
type: ComponentDefinition
persistent identity: BBa K091193
display id: BBa_K091193
version: 1
was generated by: iGEM to SBOL conversion
description: pLux/cI+pLac/Mnt+LacI+LuxR
created: 2008-08-10T11:00:00Z
modified: 2015-05-08T01:08:39Z
mutable provenance: Registry
owned by: james
owned by: myers
mutable description: This is one half of the sender-receiver device for the modified promoters. The device used AI-I and AI-2 signaling molecules to transmit information between cells.
discontinued: true
dominant: false
experience: experience/Fails
group u list: _191_
m user id: 0
owner id: 3129
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable notes: none
star: false
creator: Andrew Gordon
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_B0034
component: BBa_K091104
component: BBa_K091109
component: BBa_B0012
component: BBa_C0051
component: BBa_I13453
component: BBa_B0011
component: BBa_C0012
component: BBa_B0010
component: BBa_C0062
component: BBa_K091107
component: BBa_C0072
sequence annotation: BBa_K091107
sequence annotation: BBa_B0034
sequence annotation: BBa_K091109
sequence annotation: BBa_C0072
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_K091104
sequence annotation: BBa_C0051
sequence annotation: BBa_B0011
sequence annotation: BBa_I13453
sequence annotation: BBa_C0012
sequence annotation: BBa_C0062
sequence: https://synbiohub.org/public/igem/BBa_K091193_sequence/1

BBa_B0034
type: Component
persistent identity: component1971418
display id: component1971418
version: 1
top level: BBa_K091193
definition: BBa_B0034
access: public

BBa_K091104
type: Component
persistent identity: component1971416
display id: component1971416
version: 1
top level: BBa_K091193
definition: BBa_K091104
access: public

BBa_B0034
type: Component
persistent identity: component1971421
display id: component1971421
version: 1
top level: BBa_K091193
definition: BBa_B0034
access: public

BBa_K091109
type: Component
persistent identity: component1971419
display id: component1971419
version: 1
top level: BBa_K091193
definition: BBa_K091109
access: public

BBa_B0012
type: Component
persistent identity: component1971426
display id: component1971426
version: 1
top level: BBa_K091193
definition: BBa_B0012
access: public

BBa_C0051
type: Component
persistent identity: component1971424
display id: component1971424
version: 1
top level: BBa_K091193
definition: cI lam
access: public

BBa_I13453
type: Component
persistent identity: component1971432
display id: component1971432
version: 1
top level: BBa_K091193
definition: BBa_I13453
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BBa_B0011
type: Component
persistent identity: component1971430
display id: component1971430
version: 1
top level: BBa_K091193
definition: BBa_B0011
access: public

BBa_C0012
type: Component
persistent identity: component1971436
display id: component1971436
version: 1
top level: BBa_K091193
definition: lacI
access: public

BBa_B0034
type: Component
persistent identity: component1971434
display id: component1971434
version: 1
top level: BBa_K091193
definition: BBa_B0034
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BBa_B0012
type: Component
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display id: component1971441
version: 1
top level: BBa_K091193
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1971439
display id: component1971439
version: 1
top level: BBa_K091193
definition: BBa_B0010
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BBa_B0034
type: Component
persistent identity: component1971447
display id: component1971447
version: 1
top level: BBa_K091193
definition: BBa_B0034
access: public

BBa_I13453
type: Component
persistent identity: component1971445
display id: component1971445
version: 1
top level: BBa_K091193
definition: BBa_I13453
access: public

BBa_B0010
type: Component
persistent identity: component1971454
display id: component1971454
version: 1
top level: BBa_K091193
definition: BBa_B0010
access: public

BBa_C0062
type: Component
persistent identity: component1971450
display id: component1971450
version: 1
top level: BBa_K091193
definition: luxr
access: public

BBa_B0012
type: Component
persistent identity: component1971456
display id: component1971456
version: 1
top level: BBa_K091193
definition: BBa_B0012
access: public

BBa_K091107
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display id: component1971396
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top level: BBa_K091193
definition: BBa_K091107
access: public

BBa_K091109
type: Component
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display id: component1971399
version: 1
top level: BBa_K091193
definition: BBa_K091109
access: public

BBa_B0034
type: Component
persistent identity: component1971398
display id: component1971398
version: 1
top level: BBa_K091193
definition: BBa_B0034
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BBa_C0072
type: Component
persistent identity: component1971405
display id: component1971405
version: 1
top level: BBa_K091193
definition: mnt (s)
access: public

BBa_B0034
type: Component
persistent identity: component1971401
display id: component1971401
version: 1
top level: BBa_K091193
definition: BBa_B0034
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BBa_B0012
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display id: component1971409
version: 1
top level: BBa_K091193
definition: BBa_B0012
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BBa_B0010
type: Component
persistent identity: component1971407
display id: component1971407
version: 1
top level: BBa_K091193
definition: BBa_B0010
access: public

BBa_K091107
type: SequenceAnnotation
persistent identity: annotation1971396
display id: annotation1971396
version: 1
top level: BBa_K091193
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component: BBa_K091107

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orientation: inline

BBa_B0034
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component: BBa_B0034

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BBa_K091109
type: SequenceAnnotation
persistent identity: annotation1971399
display id: annotation1971399
version: 1
top level: BBa_K091193
location: https://synbiohub.org/public/igem/BBa_K091193/annotation1971399/range1971399/1
component: BBa_K091109

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orientation: inline

BBa_B0034
type: SequenceAnnotation
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display id: annotation1971401
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top level: BBa_K091193
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BBa_C0072
type: SequenceAnnotation
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display id: annotation1971405
version: 1
top level: BBa_K091193
location: https://synbiohub.org/public/igem/BBa_K091193/annotation1971405/range1971405/1
component: BBa_C0072

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persistent identity: range1971405
display id: range1971405
version: 1
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BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1971407
display id: annotation1971407
version: 1
top level: BBa_K091193
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component: BBa_B0010

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BBa_B0012
type: SequenceAnnotation
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display id: annotation1971409
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top level: BBa_K091193
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orientation: inline

BBa_K091104
type: SequenceAnnotation
persistent identity: annotation1971416
display id: annotation1971416
version: 1
top level: BBa_K091193
location: https://synbiohub.org/public/igem/BBa_K091193/annotation1971416/range1971416/1
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display id: range1971416
version: 1
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BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1971418
display id: annotation1971418
version: 1
top level: BBa_K091193
location: https://synbiohub.org/public/igem/BBa_K091193/annotation1971418/range1971418/1
component: BBa_B0034

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BBa_K091109
type: SequenceAnnotation
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display id: annotation1971419
version: 1
top level: BBa_K091193
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display id: range1971419
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BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1971421
display id: annotation1971421
version: 1
top level: BBa_K091193
location: https://synbiohub.org/public/igem/BBa_K091193/annotation1971421/range1971421/1
component: BBa_B0034

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persistent identity: range1971421
display id: range1971421
version: 1
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orientation: inline

BBa_C0051
type: SequenceAnnotation
persistent identity: annotation1971424
display id: annotation1971424
version: 1
top level: BBa_K091193
location: https://synbiohub.org/public/igem/BBa_K091193/annotation1971424/range1971424/1
component: BBa_C0051

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BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1971426
display id: annotation1971426
version: 1
top level: BBa_K091193
location: https://synbiohub.org/public/igem/BBa_K091193/annotation1971426/range1971426/1
component: BBa_B0012

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display id: range1971426
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orientation: inline

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation1971430
display id: annotation1971430
version: 1
top level: BBa_K091193
location: https://synbiohub.org/public/igem/BBa_K091193/annotation1971430/range1971430/1
component: BBa_B0011

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persistent identity: range1971430
display id: range1971430
version: 1
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direction: direction/forward
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orientation: inline

BBa_I13453
type: SequenceAnnotation
persistent identity: annotation1971432
display id: annotation1971432
version: 1
top level: BBa_K091193
location: https://synbiohub.org/public/igem/BBa_K091193/annotation1971432/range1971432/1
component: BBa_I13453

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persistent identity: range1971432
display id: range1971432
version: 1
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direction: direction/reverse
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orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1971434
display id: annotation1971434
version: 1
top level: BBa_K091193
location: https://synbiohub.org/public/igem/BBa_K091193/annotation1971434/range1971434/1
component: BBa_B0034

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persistent identity: range1971434
display id: range1971434
version: 1
top level: BBa_K091193
direction: direction/reverse
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orientation: inline

BBa_C0012
type: SequenceAnnotation
persistent identity: annotation1971436
display id: annotation1971436
version: 1
top level: BBa_K091193
location: https://synbiohub.org/public/igem/BBa_K091193/annotation1971436/range1971436/1
component: BBa_C0012

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persistent identity: range1971436
display id: range1971436
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orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1971439
display id: annotation1971439
version: 1
top level: BBa_K091193
location: https://synbiohub.org/public/igem/BBa_K091193/annotation1971439/range1971439/1
component: BBa_B0010

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display id: range1971439
version: 1
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BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1971441
display id: annotation1971441
version: 1
top level: BBa_K091193
location: https://synbiohub.org/public/igem/BBa_K091193/annotation1971441/range1971441/1
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display id: range1971441
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BBa_I13453
type: SequenceAnnotation
persistent identity: annotation1971445
display id: annotation1971445
version: 1
top level: BBa_K091193
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component: BBa_I13453

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BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1971447
display id: annotation1971447
version: 1
top level: BBa_K091193
location: https://synbiohub.org/public/igem/BBa_K091193/annotation1971447/range1971447/1
component: BBa_B0034

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display id: range1971447
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BBa_C0062
type: SequenceAnnotation
persistent identity: annotation1971450
display id: annotation1971450
version: 1
top level: BBa_K091193
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BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1971454
display id: annotation1971454
version: 1
top level: BBa_K091193
location: https://synbiohub.org/public/igem/BBa_K091193/annotation1971454/range1971454/1
component: BBa_B0010

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display id: range1971454
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BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1971456
display id: annotation1971456
version: 1
top level: BBa_K091193
location: https://synbiohub.org/public/igem/BBa_K091193/annotation1971456/range1971456/1
component: BBa_B0012

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display id: range1971456
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BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

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type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0011
type: ComponentDefinition
persistent identity: BBa B0011
display id: BBa_B0011
version: 1
was generated by: iGEM to SBOL conversion
description: LuxICDABEG (+/-)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from luxICDABEG operon terminator of Vibrio fischeri <genbank>AF170104</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Bidirectional transcriptional terminator consisting of a 22 bp stem-loop.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: In the naturally-occuring sequence there is a mismatch in the stem of the stem loop. This can be corrected via an A->G mutation (at position 40 -- sequence coordinate/not MFOLD coordinate). The above sequence does not reflect this mutation (but the MFOLD image does). This terminator's location cannot be found using some inverted repeat detectors like PALINDROME because it is too short and contains a mismatch. This one was found with the help of Tom Knight. It lies between two coding regions that point towards eachother.
star: true
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0011
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0011_sequence/1

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation7019
display id: annotation7019
version: 1
top level: BBa_B0011
location: https://synbiohub.org/public/igem/BBa_B0011/annotation7019/range7019/1
role: SO:0000804
role: feature/BioBrick

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type: Range
persistent identity: range7019
display id: range7019
version: 1
top level: BBa_B0011
direction: direction/forward
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orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation1683
display id: annotation1683
version: 1
top level: BBa_B0011
location: https://synbiohub.org/public/igem/BBa_B0011/annotation1683/range1683/1
role: SO:0000313
role: feature/stem loop

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type: Range
persistent identity: range1683
display id: range1683
version: 1
top level: BBa_B0011
direction: direction/forward
start: 13
end: 35
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

cI lam
type: ComponentDefinition
persistent identity: BBa C0051
display id: BBa_C0051
version: 1
was generated by: iGEM to SBOL conversion
description: cI repressor from E. coli phage lambda (+LVA)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999).
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Coding region for the cI repressor based on cI repressor from bacteriophage lambda modified with an LVA tail for rapid degradation of the protein. cI repressor binds to the cI regulator (BBa_R0051).
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: References (unparsed) here: A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000 Octamerization of CI repressor is needed for effective repression of PRM and efficient switching from lysogeny. Ian B. Dodd,1 Alison J. Perkins, Daniel Tsemitsidis, and J. Barry Egan , Genes and Development (Vol 15, No. 22) 3013-3022: 2001 References (unparsed) here: A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000 Octamerization of CI repressor is needed for effective repression of PRM and efficient switching from lysogeny. Ian B. Dodd,1 Alison J. Perkins, Daniel Tsemitsidis, and J. Barry Egan , Genes and Development (Vol 15, No. 22) 3013-3022: 2001 BBa_C0051 cI repressor is based on the cI repressor from the Elowitz's repressilator. It has been modified to include a rapid degradation LAA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.
star: true
creator: Vinay S Mahajan, Brian Chow, Peter Carr, Voichita Marinescu and Alexander D. Wissner-Gross
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: cI lambda
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0051_sequence/1

cI lambda
type: SequenceAnnotation
persistent identity: annotation23334
display id: annotation23334
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_C0051/annotation23334/range23334/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_C0051/annotation23334/range23334/1
type: Range
persistent identity: range23334
display id: range23334
version: 1
top level: cI lam
direction: direction/forward
start: 4
end: 711
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation23335
display id: annotation23335
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_C0051/annotation23335/range23335/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_C0051/annotation23335/range23335/1
type: Range
persistent identity: range23335
display id: range23335
version: 1
top level: cI lam
direction: direction/forward
start: 712
end: 744
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213991
display id: annotation2213991
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_C0051/annotation2213991/range2213991/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_C0051/annotation2213991/range2213991/1
type: Range
persistent identity: range2213991
display id: range2213991
version: 1
top level: cI lam
direction: direction/forward
start: 751
end: 775
orientation: inline

lacI
type: ComponentDefinition
persistent identity: BBa C0012
display id: BBa_C0012
version: 1
was generated by: iGEM to SBOL conversion
description: lacI repressor from E. coli (+LVA)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: represillator of Elowitz and Leibler (2000)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Coding region for the LacI protein with an LVA degradation tail and without an RBS. LacI binds to the pLac regulator <bb_part>BBa_R0010</bb_part> and PLlac01 hybrid regulator <bb_part>BBa_R0011</bb_part> and inhibits transcription. IPTG (Isopropylthiogalactoside) binds to LacI and inhibits its operation, therefore promoting transcription. A rapid degredation tail (LVA) has been added to improve the High to Low performance of this part.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: References (unparsed) here: Elowitz, M.B., Leibler, S. A synthetic oscillatory network of transcriptional regulators. Nature 403, 335-338 (2000). [ELOW00] References (unparsed) here: Elowitz, M.B., Leibler, S. A synthetic oscillatory network of transcriptional regulators. Nature 403, 335-338 (2000). [ELOW00] Sequence taken from the repressilator of Elowitz and Leibler (2000). The obtained sequence was compared to the wild-type sequence for LacI obtained through a database search. The sequence had been modified from the wild-type in that wild-type GTG start was changed to an ATG start (note, actual ORF in E.coli has several GTG starts it would seem). The LVA tag has been added for quicker degradation. Incompatible with systems containing LacI, lactose, or IPTG.
star: true
creator: Grace Kenney, Daniel Shen, Neelaksh Varshney, Samantha Sutton
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: BBa_C0012
sequence annotation: lacI-LVA
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0012_sequence/1

BBa_C0012
type: SequenceAnnotation
persistent identity: annotation7031
display id: annotation7031
version: 1
top level: lacI
location: https://synbiohub.org/public/igem/BBa_C0012/annotation7031/range7031/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_C0012/annotation7031/range7031/1
type: Range
persistent identity: range7031
display id: range7031
version: 1
top level: lacI
direction: direction/forward
start: 1
end: 1128
orientation: inline

lacI-LVA
type: SequenceAnnotation
persistent identity: annotation1723
display id: annotation1723
version: 1
top level: lacI
location: https://synbiohub.org/public/igem/BBa_C0012/annotation1723/range1723/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_C0012/annotation1723/range1723/1
type: Range
persistent identity: range1723
display id: range1723
version: 1
top level: lacI
direction: direction/forward
start: 1
end: 1128
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation1722
display id: annotation1722
version: 1
top level: lacI
location: https://synbiohub.org/public/igem/BBa_C0012/annotation1722/range1722/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_C0012/annotation1722/range1722/1
type: Range
persistent identity: range1722
display id: range1722
version: 1
top level: lacI
direction: direction/forward
start: 1090
end: 1128
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213988
display id: annotation2213988
version: 1
top level: lacI
location: https://synbiohub.org/public/igem/BBa_C0012/annotation2213988/range2213988/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_C0012/annotation2213988/range2213988/1
type: Range
persistent identity: range2213988
display id: range2213988
version: 1
top level: lacI
direction: direction/forward
start: 1129
end: 1153
orientation: inline

BBa_I13453
type: ComponentDefinition
persistent identity: BBa I13453
display id: BBa_I13453
version: 1
was generated by: iGEM to SBOL conversion
description: Pbad promoter
created: 2005-05-24T11:00:00Z
modified: 2015-08-31T04:07:34Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: PBad promoter from I0500 without AraC.
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jkm
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I13453_sequence/1

BBa_K091104
type: ComponentDefinition
persistent identity: BBa K091104
display id: BBa_K091104
version: 1
was generated by: iGEM to SBOL conversion
description: pLac/Mnt Hybrid Promoter
created: 2008-06-18T11:00:00Z
modified: 2015-05-08T01:08:37Z
mutable provenance: This part comes from the Mnt promoter and the LacI promoter
owned by: james
owned by: myers
mutable description: This promoter is a modified version of the Mnt promoter that is also responsive to LacI. The promoter should be repressed by Mnt repressor. It should also be repressed by LacI, and in the absence of Mnt repressor, should be induced by IPTG.
discontinued: false
dominant: false
experience: experience/None
group u list: _191_
m user id: 0
owner id: 3080
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: Mnt repressor binds as a tetramer to two half-operator sites. Introduction of a LacI binding site between the Mnt promoter -10 sequence and the start site for transcription should allow for repression by LacI. Accordingly, the hybrid promoter was designed by 1) Remove all bases of mnt promoter 3??? to -10: gagtcgtattaattt will be replaced by tgtgtggaattgtga, and 2) Position lacI binding site (composed of an inverted repeat) from the lacI regulated promoter (R0010) immediately 3??? to truncated mnt promoter.
star: true
creator: Robert Cool
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: Mnt
sequence annotation: -10
sequence annotation: LacI
sequence: https://synbiohub.org/public/igem/BBa_K091104_sequence/1

Mnt
type: SequenceAnnotation
persistent identity: annotation1963743
display id: annotation1963743
version: 1
top level: BBa_K091104
location: https://synbiohub.org/public/igem/BBa_K091104/annotation1963743/range1963743/1
role: feature/operator
role: SO:0000057

https://synbiohub.org/public/igem/BBa_K091104/annotation1963743/range1963743/1
type: Range
persistent identity: range1963743
display id: range1963743
version: 1
top level: BBa_K091104
direction: direction/forward
start: 1
end: 52
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1963737
display id: annotation1963737
version: 1
top level: BBa_K091104
location: https://synbiohub.org/public/igem/BBa_K091104/annotation1963737/range1963737/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_K091104/annotation1963737/range1963737/1
type: Range
persistent identity: range1963737
display id: range1963737
version: 1
top level: BBa_K091104
direction: direction/forward
start: 47
end: 52
orientation: inline

LacI
type: SequenceAnnotation
persistent identity: annotation1963738
display id: annotation1963738
version: 1
top level: BBa_K091104
location: https://synbiohub.org/public/igem/BBa_K091104/annotation1963738/range1963738/1
role: feature/operator
role: SO:0000057

https://synbiohub.org/public/igem/BBa_K091104/annotation1963738/range1963738/1
type: Range
persistent identity: range1963738
display id: range1963738
version: 1
top level: BBa_K091104
direction: direction/forward
start: 53
end: 87
orientation: inline

BBa_K091109
type: ComponentDefinition
persistent identity: BBa K091109
display id: BBa_K091109
version: 1
was generated by: iGEM to SBOL conversion
description: LuxS
created: 2008-06-18T11:00:00Z
modified: 2015-05-08T01:08:37Z
mutable provenance: The LuxS sequence was pulled from E. coli MG1655 by predesigned primers.
owned by: james
owned by: myers
mutable description: LuxS is a synthase for the AI-2 quorum sensing system. This protein is involved in the production of small molecules that are readily absorbed by multiple species of bacteria. LuxS can be used as part of a positive feedback loop in cell to cell signaling.
discontinued: false
dominant: false
experience: experience/Works
group u list: _191_
m user id: 0
owner id: 3129
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Nucleotide position 54 changed from A to T in order to destroy a PstI site; this is a sense mutation Also changed stop codon from TAG to TAA (recommended in Registry Help)
star: false
creator: Andrew Gordon
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K091109_sequence/1

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

luxr
type: ComponentDefinition
persistent identity: BBa C0062
display id: BBa_C0062
version: 1
was generated by: iGEM to SBOL conversion
description: luxR repressor/activator, (no LVA?)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: V. fischeri <genbank>AF170104</genbank>
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: In complex with HSL, LuxR binds to the Lux promoter, activating transcription from Pr <bb_part>BBa_R0062</bb_part>, and repressing transcription from Pl <bb_part>BBa_R0063</bb_part>. The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the Lux activator, LuxR complexed to HSL. Two molecules of LuxR protein form a complex with two molecules the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: 2 silent point mutants were introduced in the coding sequence to remove internal XbaI and PstI sites. Mutation sites were chosen to replace codons commonly used in E. coli with codons used at a similar frequency.
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: luxR
sequence annotation: prefix
sequence annotation: BBa_C0062
sequence annotation: T
sequence annotation: A
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0062_sequence/1

luxR
type: SequenceAnnotation
persistent identity: annotation1766
display id: annotation1766
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1766/range1766/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_C0062/annotation1766/range1766/1
type: Range
persistent identity: range1766
display id: range1766
version: 1
top level: luxr
direction: direction/forward
start: 1
end: 750
orientation: inline

prefix
type: SequenceAnnotation
persistent identity: annotation1762
display id: annotation1762
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1762/range1762/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_C0062/annotation1762/range1762/1
type: Range
persistent identity: range1762
display id: range1762
version: 1
top level: luxr
direction: direction/forward
start: 1
end: 2
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation7039
display id: annotation7039
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation7039/range7039/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_C0062/annotation7039/range7039/1
type: Range
persistent identity: range7039
display id: range7039
version: 1
top level: luxr
direction: direction/forward
start: 1
end: 756
orientation: inline

T
type: SequenceAnnotation
persistent identity: annotation1764
display id: annotation1764
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1764/range1764/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_C0062/annotation1764/range1764/1
type: Range
persistent identity: range1764
display id: range1764
version: 1
top level: luxr
direction: direction/forward
start: 174
end: 174
orientation: inline

A
type: SequenceAnnotation
persistent identity: annotation1765
display id: annotation1765
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1765/range1765/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_C0062/annotation1765/range1765/1
type: Range
persistent identity: range1765
display id: range1765
version: 1
top level: luxr
direction: direction/forward
start: 492
end: 492
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213986
display id: annotation2213986
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation2213986/range2213986/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_C0062/annotation2213986/range2213986/1
type: Range
persistent identity: range2213986
display id: range2213986
version: 1
top level: luxr
direction: direction/forward
start: 757
end: 781
orientation: inline

BBa_K091107
type: ComponentDefinition
persistent identity: BBa K091107
display id: BBa_K091107
version: 1
was generated by: iGEM to SBOL conversion
description: pLux/cI Hybrid Promoter
created: 2008-06-18T11:00:00Z
modified: 2015-05-08T01:08:37Z
mutable provenance: this part comes from LuxR promoter and cI lambda promoter.
owned by: james
owned by: myers
mutable description: This hybrid promoter is designed to respond to activation by LuxR when it is bound to AHL and to repression by the phage lambda cI repressor. The repression needs to win over activation.
discontinued: false
dominant: false
experience: experience/None
group u list: _191_
m user id: 0
owner id: 3130
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: This part is based on the idea of replacing the region of the Lux promoter downstream of the -35 with the OR2 cI half-operator, added LuxR-10 region. Based on what we have found up to now, promoters which are constructed just by inserting OR1/2 downstream of Lux are all very leaky.
star: true
creator: Xiao Zhu
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Lux Box
sequence annotation: Lux -35
sequence annotation: cI OR2
sequence annotation: Lux -10
sequence: https://synbiohub.org/public/igem/BBa_K091107_sequence/1

Lux Box
type: SequenceAnnotation
persistent identity: annotation1963750
display id: annotation1963750
version: 1
top level: BBa_K091107
location: https://synbiohub.org/public/igem/BBa_K091107/annotation1963750/range1963750/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_K091107/annotation1963750/range1963750/1
type: Range
persistent identity: range1963750
display id: range1963750
version: 1
top level: BBa_K091107
direction: direction/forward
start: 1
end: 20
orientation: inline

Lux -35
type: SequenceAnnotation
persistent identity: annotation1963751
display id: annotation1963751
version: 1
top level: BBa_K091107
location: https://synbiohub.org/public/igem/BBa_K091107/annotation1963751/range1963751/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K091107/annotation1963751/range1963751/1
type: Range
persistent identity: range1963751
display id: range1963751
version: 1
top level: BBa_K091107
direction: direction/forward
start: 20
end: 25
orientation: inline

cI OR2
type: SequenceAnnotation
persistent identity: annotation1963752
display id: annotation1963752
version: 1
top level: BBa_K091107
location: https://synbiohub.org/public/igem/BBa_K091107/annotation1963752/range1963752/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_K091107/annotation1963752/range1963752/1
type: Range
persistent identity: range1963752
display id: range1963752
version: 1
top level: BBa_K091107
direction: direction/forward
start: 26
end: 43
orientation: inline

Lux -10
type: SequenceAnnotation
persistent identity: annotation1963753
display id: annotation1963753
version: 1
top level: BBa_K091107
location: https://synbiohub.org/public/igem/BBa_K091107/annotation1963753/range1963753/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K091107/annotation1963753/range1963753/1
type: Range
persistent identity: range1963753
display id: range1963753
version: 1
top level: BBa_K091107
direction: direction/forward
start: 44
end: 49
orientation: inline

mnt (s)
type: ComponentDefinition
persistent identity: BBa C0072
display id: BBa_C0072
version: 1
was generated by: iGEM to SBOL conversion
description: mnt repressor (strong) from Salmonella phage P22 (+LVA)
created: 2004-01-28T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: enterobacteriophage p22
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: -- No description --
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: wild type dimeric repressor from enterobacteriophage p22 with dissociation constant of 2.5*10^-12. sequence was translated with the E.Coli K12 codon usage table, and nucleotides 95-97 was changed from AAT to AAC to avoid the cut site GAATTC (94-99). Identification of Functionally Important Residues in the DNA Binding Region of the MNT Repressor Kendall, KL; Sauer, RT The Journal of Biological Chemistry, 264(23):13706-13710, 1989
star: true
creator: crackdots
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: mnt
sequence annotation: T
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0072_sequence/1

mnt
type: SequenceAnnotation
persistent identity: annotation308499
display id: annotation308499
version: 1
top level: mnt (s)
location: https://synbiohub.org/public/igem/BBa_C0072/annotation308499/range308499/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_C0072/annotation308499/range308499/1
type: Range
persistent identity: range308499
display id: range308499
version: 1
top level: mnt (s)
direction: direction/forward
start: 1
end: 249
orientation: inline

T
type: SequenceAnnotation
persistent identity: annotation308502
display id: annotation308502
version: 1
top level: mnt (s)
location: https://synbiohub.org/public/igem/BBa_C0072/annotation308502/range308502/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_C0072/annotation308502/range308502/1
type: Range
persistent identity: range308502
display id: range308502
version: 1
top level: mnt (s)
direction: direction/forward
start: 95
end: 97
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation308503
display id: annotation308503
version: 1
top level: mnt (s)
location: https://synbiohub.org/public/igem/BBa_C0072/annotation308503/range308503/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_C0072/annotation308503/range308503/1
type: Range
persistent identity: range308503
display id: range308503
version: 1
top level: mnt (s)
direction: direction/forward
start: 250
end: 282
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214010
display id: annotation2214010
version: 1
top level: mnt (s)
location: https://synbiohub.org/public/igem/BBa_C0072/annotation2214010/range2214010/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_C0072/annotation2214010/range2214010/1
type: Range
persistent identity: range2214010
display id: range2214010
version: 1
top level: mnt (s)
direction: direction/forward
start: 289
end: 313
orientation: inline

https://synbiohub.org/public/igem/BBa_K091193_sequence/1
type: Sequence
persistent identity: BBa K091193 sequence
display id: BBa_K091193_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acctgtaggatcgtacaggtttacgtaacaccgtgcgtgttgatatagtcgaataaatactagagaaagaggagaaatactagatgccgttgttagatagcttcacagtcgatcatacccggatggaagcgcctgctgttcgggtggcgaaaacaatgaacaccccgcatggcgacgcaatcaccgtgttcgatctgcgcttctgcgtgccgaacaaagaagtgatgccagaaagagggatccataccctggagcacctgtttgctggttttatgcgtaaccatcttaacggtaatggtgtagagattatcgatatctcgccaatgggctgccgcaccggtttttatatgagtctgattggtacgccagatgagcagcgtgttgctgatgcctggaaagcggcaatggaagacgtgctgaaagtgcaggatcagaatcagatcccggaactgaacgtctaccagtgtggcacttaccagatgcactcgttgcaggaagcgcaggatattgcgcgtagcattctggaacgtgacgtacgcatcaacagcaacgaagaactggcactgccgaaagagaagttgcaggaactgcacatctaatactagagaaagaggagaaatactagatggcccgggatgatcctcacttcaattttcgtatgccaatggaagtaagagagaaattgaaatttagagcagaggcaaacggacggagcatgaactctgagcttttgcaaatcgtacaagatgccctaagcaaaccgtcaccagtcactgggtaccgcaatgatgcggaacgactcgccgatgagcagagcgagttagtgaagaagatggtcttcgatacactgaaggatctttataaaaaaaccaccgctgcaaacgacgaaaactacgctttagtagcttaataaccctgatagtgctagtgtagatccctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagctcgaggtgagtgcacagtactaggtccacggtgacctagatctcctatagttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgccgttgttagatagcttcacagtcgatcatacccggatggaagcgcctgctgttcgggtggcgaaaacaatgaacaccccgcatggcgacgcaatcaccgtgttcgatctgcgcttctgcgtgccgaacaaagaagtgatgccagaaagagggatccataccctggagcacctgtttgctggttttatgcgtaaccatcttaacggtaatggtgtagagattatcgatatctcgccaatgggctgccgcaccggtttttatatgagtctgattggtacgccagatgagcagcgtgttgctgatgcctggaaagcggcaatggaagacgtgctgaaagtgcaggatcagaatcagatcccggaactgaacgtctaccagtgtggcacttaccagatgcactcgttgcaggaagcgcaggatattgcgcgtagcattctggaacgtgacgtacgcatcaacagcaacgaagaactggcactgccgaaagagaagttgcaggaactgcacatctaatactagagaaagaggagaaatactagatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgaga…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0011_sequence/1
type: Sequence
persistent identity: BBa B0011 sequence
display id: BBa_B0011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agagaatataaaaagccagattattaatccggcttttttattattt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0051_sequence/1
type: Sequence
persistent identity: BBa C0051 sequence
display id: BBa_C0051_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0012_sequence/1
type: Sequence
persistent identity: BBa C0012 sequence
display id: BBa_C0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I13453_sequence/1
type: Sequence
persistent identity: BBa I13453 sequence
display id: BBa_I13453_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K091104_sequence/1
type: Sequence
persistent identity: BBa K091104 sequence
display id: BBa_K091104_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctcgaggtgagtgcacagtactaggtccacggtgacctagatctcctatagttgtgtggaattgtgagcggataacaatttcacaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K091109_sequence/1
type: Sequence
persistent identity: BBa K091109 sequence
display id: BBa_K091109_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgccgttgttagatagcttcacagtcgatcatacccggatggaagcgcctgctgttcgggtggcgaaaacaatgaacaccccgcatggcgacgcaatcaccgtgttcgatctgcgcttctgcgtgccgaacaaagaagtgatgccagaaagagggatccataccctggagcacctgtttgctggttttatgcgtaaccatcttaacggtaatggtgtagagattatcgatatctcgccaatgggctgccgcaccggtttttatatgagtctgattggtacgccagatgagcagcgtgttgctgatgcctggaaagcggcaatggaagacgtgctgaaagtgcaggatcagaatcagatcccggaactgaacgtctaccagtgtggcacttaccagatgcactcgttgcaggaagcgcaggatattgcgcgtagcattctggaacgtgacgtacgcatcaacagcaacgaagaactggcactgccgaaagagaagttgcaggaactgcacatctaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0062_sequence/1
type: Sequence
persistent identity: BBa C0062 sequence
display id: BBa_C0062_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K091107_sequence/1
type: Sequence
persistent identity: BBa K091107 sequence
display id: BBa_K091107_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acctgtaggatcgtacaggtttacgtaacaccgtgcgtgttgatatagtcgaataaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0072_sequence/1
type: Sequence
persistent identity: BBa C0072 sequence
display id: BBa_C0072_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcccgggatgatcctcacttcaattttcgtatgccaatggaagtaagagagaaattgaaatttagagcagaggcaaacggacggagcatgaactctgagcttttgcaaatcgtacaagatgccctaagcaaaccgtcaccagtcactgggtaccgcaatgatgcggaacgactcgccgatgagcagagcgagttagtgaagaagatggtcttcgatacactgaaggatctttataaaaaaaccaccgctgcaaacgacgaaaactacgctttagtagcttaataaccctgatagtgctagtgtagatccc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z