BBa_J119077
type: ComponentDefinition
persistent identity: BBa J119077
display id: BBa_J119077
version: 1
was generated by: iGEM to SBOL conversion
description: tRNA-pLacI-RBS-T7RNAP (mutant 4 and tRNA 91)
created: 2012-03-08T12:00:00Z
modified: 2015-08-31T04:08:27Z
mutable provenance: MWSU and Davidson
owned by: james
owned by: myers
mutable description: This part contains T7RNAP with an inserted oligo causing a frameshift mutation. The tRNA is a five base frameshift suppressor.
discontinued: false
dominant: false
experience: experience/None
group u list: _613_
m user id: 0
owner id: 10415
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Michel Conn
type: DnaRegion
role: partType/Project
role: SO:0000804
component: BBa_K199191
component: BBa_J119058
sequence annotation: BBa_K199191
sequence annotation: BBa_J119058
sequence: https://synbiohub.org/public/igem/BBa_J119077_sequence/1

BBa_K199191
type: Component
persistent identity: component2170531
display id: component2170531
version: 1
top level: BBa_J119077
definition: BBa_K199191
access: public

BBa_J119058
type: Component
persistent identity: component2170536
display id: component2170536
version: 1
top level: BBa_J119077
definition: BBa_J119058
access: public

BBa_K199191
type: SequenceAnnotation
persistent identity: annotation2170531
display id: annotation2170531
version: 1
top level: BBa_J119077
location: https://synbiohub.org/public/igem/BBa_J119077/annotation2170531/range2170531/1
component: BBa_K199191

https://synbiohub.org/public/igem/BBa_J119077/annotation2170531/range2170531/1
type: Range
persistent identity: range2170531
display id: range2170531
version: 1
top level: BBa_J119077
direction: direction/reverse
start: 1
end: 1069
orientation: inline

BBa_J119058
type: SequenceAnnotation
persistent identity: annotation2170536
display id: annotation2170536
version: 1
top level: BBa_J119077
location: https://synbiohub.org/public/igem/BBa_J119077/annotation2170536/range2170536/1
component: BBa_J119058

https://synbiohub.org/public/igem/BBa_J119077/annotation2170536/range2170536/1
type: Range
persistent identity: range2170536
display id: range2170536
version: 1
top level: BBa_J119077
direction: direction/reverse
start: 1078
end: 3904
orientation: inline

BBa_J119058
type: ComponentDefinition
persistent identity: BBa J119058
display id: BBa_J119058
version: 1
was generated by: iGEM to SBOL conversion
description: pLacI-RBS-T7 RNAP (2SAT mutant 4)
created: 2012-01-26T12:00:00Z
modified: 2015-08-31T04:08:26Z
mutable provenance: Existing part and synthetic oligos.
owned by: james
owned by: myers
mutable description: T7 RNAP gene has frameshift mutation inserted that prevents it from being expressed and causing RFP expression. Suppression by 5 nt anticodon tRNAs is expected to rescue the RFP phenotype. The frameshift mutation is of the form (a??? or b) and (a or c???).
discontinued: false
dominant: false
experience: experience/None
group u list: _613_435_
m user id: 0
owner id: 606
owning group id: 61
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: The frameshift mutation is of the form (a??? or b) and (a or c???).
star: false
creator: Cody Barta
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: pLacI
sequence annotation: B0030
sequence annotation: T7 RNAP
sequence annotation: 22 nt frameshift mutation
sequence: https://synbiohub.org/public/igem/BBa_J119058_sequence/1

pLacI
type: SequenceAnnotation
persistent identity: annotation2169869
display id: annotation2169869
version: 1
top level: BBa_J119058
location: https://synbiohub.org/public/igem/BBa_J119058/annotation2169869/range2169869/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_J119058/annotation2169869/range2169869/1
type: Range
persistent identity: range2169869
display id: range2169869
version: 1
top level: BBa_J119058
direction: direction/forward
start: 1
end: 56
orientation: inline

B0030
type: SequenceAnnotation
persistent identity: annotation2169870
display id: annotation2169870
version: 1
top level: BBa_J119058
location: https://synbiohub.org/public/igem/BBa_J119058/annotation2169870/range2169870/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_J119058/annotation2169870/range2169870/1
type: Range
persistent identity: range2169870
display id: range2169870
version: 1
top level: BBa_J119058
direction: direction/forward
start: 68
end: 82
orientation: inline

T7 RNAP
type: SequenceAnnotation
persistent identity: annotation2169871
display id: annotation2169871
version: 1
top level: BBa_J119058
location: https://synbiohub.org/public/igem/BBa_J119058/annotation2169871/range2169871/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_J119058/annotation2169871/range2169871/1
type: Range
persistent identity: range2169871
display id: range2169871
version: 1
top level: BBa_J119058
direction: direction/forward
start: 89
end: 2810
orientation: inline

22 nt frameshift mutation
type: SequenceAnnotation
persistent identity: annotation2169872
display id: annotation2169872
version: 1
top level: BBa_J119058
location: https://synbiohub.org/public/igem/BBa_J119058/annotation2169872/range2169872/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_J119058/annotation2169872/range2169872/1
type: Range
persistent identity: range2169872
display id: range2169872
version: 1
top level: BBa_J119058
direction: direction/forward
start: 115
end: 126
orientation: inline

BBa_K199191
type: ComponentDefinition
persistent identity: BBa K199191
display id: BBa_K199191
version: 1
was generated by: iGEM to SBOL conversion
description: pLac+tRNA CCACC+pLac+tRNA AGGAC+pLac+tRNA CUACC
created: 2010-07-21T11:00:00Z
modified: 2015-05-08T01:11:21Z
mutable provenance: BioBricks
owned by: james
owned by: myers
mutable description: pLac+tRNA CCACC+pLac+tRNA AGGAC+pLac+tRNA CUACC
discontinued: false
dominant: false
group u list: _295_
m user id: 0
owner id: 5111
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: N/A
star: false
creator: Eric Sawyer
type: DnaRegion
role: SO:0000834
role: partType/RNA
component: BBa_K199014
component: BBa_R0010
component: BBa_K199048
component: BBa_K199045
sequence annotation: BBa_R0010
sequence annotation: BBa_K199048
sequence annotation: BBa_K199014
sequence annotation: BBa_K199045
sequence: https://synbiohub.org/public/igem/BBa_K199191_sequence/1

BBa_K199014
type: Component
persistent identity: component2075361
display id: component2075361
version: 1
top level: BBa_K199191
definition: BBa_K199014
access: public

BBa_R0010
type: Component
persistent identity: component2075344
display id: component2075344
version: 1
top level: BBa_K199191
definition: LacI
access: public

BBa_R0010
type: Component
persistent identity: component2075353
display id: component2075353
version: 1
top level: BBa_K199191
definition: LacI
access: public

BBa_K199048
type: Component
persistent identity: component2075352
display id: component2075352
version: 1
top level: BBa_K199191
definition: BBa_K199048
access: public

BBa_R0010
type: Component
persistent identity: component2075362
display id: component2075362
version: 1
top level: BBa_K199191
definition: LacI
access: public

BBa_K199045
type: Component
persistent identity: component2075370
display id: component2075370
version: 1
top level: BBa_K199191
definition: BBa_K199045
access: public

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2075344
display id: annotation2075344
version: 1
top level: BBa_K199191
location: https://synbiohub.org/public/igem/BBa_K199191/annotation2075344/range2075344/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K199191/annotation2075344/range2075344/1
type: Range
persistent identity: range2075344
display id: range2075344
version: 1
top level: BBa_K199191
direction: direction/forward
start: 1
end: 200
orientation: inline

BBa_K199048
type: SequenceAnnotation
persistent identity: annotation2075352
display id: annotation2075352
version: 1
top level: BBa_K199191
location: https://synbiohub.org/public/igem/BBa_K199191/annotation2075352/range2075352/1
component: BBa_K199048

https://synbiohub.org/public/igem/BBa_K199191/annotation2075352/range2075352/1
type: Range
persistent identity: range2075352
display id: range2075352
version: 1
top level: BBa_K199191
direction: direction/reverse
start: 209
end: 351
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2075353
display id: annotation2075353
version: 1
top level: BBa_K199191
location: https://synbiohub.org/public/igem/BBa_K199191/annotation2075353/range2075353/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K199191/annotation2075353/range2075353/1
type: Range
persistent identity: range2075353
display id: range2075353
version: 1
top level: BBa_K199191
direction: direction/forward
start: 360
end: 559
orientation: inline

BBa_K199014
type: SequenceAnnotation
persistent identity: annotation2075361
display id: annotation2075361
version: 1
top level: BBa_K199191
location: https://synbiohub.org/public/igem/BBa_K199191/annotation2075361/range2075361/1
component: BBa_K199014

https://synbiohub.org/public/igem/BBa_K199191/annotation2075361/range2075361/1
type: Range
persistent identity: range2075361
display id: range2075361
version: 1
top level: BBa_K199191
direction: direction/reverse
start: 568
end: 710
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2075362
display id: annotation2075362
version: 1
top level: BBa_K199191
location: https://synbiohub.org/public/igem/BBa_K199191/annotation2075362/range2075362/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K199191/annotation2075362/range2075362/1
type: Range
persistent identity: range2075362
display id: range2075362
version: 1
top level: BBa_K199191
direction: direction/forward
start: 719
end: 918
orientation: inline

BBa_K199045
type: SequenceAnnotation
persistent identity: annotation2075370
display id: annotation2075370
version: 1
top level: BBa_K199191
location: https://synbiohub.org/public/igem/BBa_K199191/annotation2075370/range2075370/1
component: BBa_K199045

https://synbiohub.org/public/igem/BBa_K199191/annotation2075370/range2075370/1
type: Range
persistent identity: range2075370
display id: range2075370
version: 1
top level: BBa_K199191
direction: direction/reverse
start: 927
end: 1069
orientation: inline

LacI
type: ComponentDefinition
persistent identity: BBa R0010
display id: BBa_R0010
version: 1
was generated by: iGEM to SBOL conversion
description: promoter (lacI regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
star: true
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: end of LacI coding region (inactive)
sequence annotation: BBa_R0010
sequence annotation: CAP binding site
sequence annotation: -35
sequence annotation: -10
sequence annotation: LacI binding site
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_R0010_sequence/1

end of LacI coding region (inactive)
type: SequenceAnnotation
persistent identity: annotation1961221
display id: annotation1961221
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
type: Range
persistent identity: range1961221
display id: range1961221
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 88
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1961222
display id: annotation1961222
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
type: Range
persistent identity: range1961222
display id: range1961222
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 200
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation1961223
display id: annotation1961223
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
type: Range
persistent identity: range1961223
display id: range1961223
version: 1
top level: LacI
direction: direction/forward
start: 89
end: 126
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1961224
display id: annotation1961224
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
type: Range
persistent identity: range1961224
display id: range1961224
version: 1
top level: LacI
direction: direction/forward
start: 137
end: 142
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1961225
display id: annotation1961225
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
type: Range
persistent identity: range1961225
display id: range1961225
version: 1
top level: LacI
direction: direction/forward
start: 161
end: 166
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation1961226
display id: annotation1961226
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
type: Range
persistent identity: range1961226
display id: range1961226
version: 1
top level: LacI
direction: direction/forward
start: 166
end: 200
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1961227
display id: annotation1961227
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
type: Range
persistent identity: range1961227
display id: range1961227
version: 1
top level: LacI
direction: direction/forward
start: 173
end: 173
orientation: inline

BBa_K199014
type: ComponentDefinition
persistent identity: BBa K199014
display id: BBa_K199014
version: 1
was generated by: iGEM to SBOL conversion
description: AGGAC tRNA Suppressor (Produces Serine)
created: 2009-07-01T11:00:00Z
modified: 2015-05-08T01:11:18Z
mutable provenance: Synthesized from oligonucleotides and sequence verified.
owned by: james
owned by: myers
mutable description: A serine tRNA was modified to have the anticodon loop 5' CUGUCCUAA 3'so that the anticodon GUCCA could be used to decode a frameshift mutation of AGGAC in a mRNA.
discontinued: false
dominant: false
experience: experience/None
group u list: _295_
m user id: 0
owner id: 5115
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: None.
star: false
creator: William Vernon
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence annotation: 5 nt anticodon
sequence: https://synbiohub.org/public/igem/BBa_K199014_sequence/1

5 nt anticodon
type: SequenceAnnotation
persistent identity: annotation2007934
display id: annotation2007934
version: 1
top level: BBa_K199014
location: https://synbiohub.org/public/igem/BBa_K199014/annotation2007934/range2007934/1
role: feature/mutation
role: SO:0001059

https://synbiohub.org/public/igem/BBa_K199014/annotation2007934/range2007934/1
type: Range
persistent identity: range2007934
display id: range2007934
version: 1
top level: BBa_K199014
direction: direction/forward
start: 46
end: 50
orientation: inline

BBa_K199045
type: ComponentDefinition
persistent identity: BBa K199045
display id: BBa_K199045
version: 1
was generated by: iGEM to SBOL conversion
description: CUACC tRNA Suppressor (Produces Serine)
created: 2009-07-19T11:00:00Z
modified: 2015-05-08T01:11:19Z
mutable provenance: Assembly from oligos
owned by: james
owned by: myers
mutable description: A serine tRNA was modified to have the anticodon loop 5' GUGGUAGAA 3'so that the anticodon GGUAG could be used to decode a frameshift mutation of CUACC in a mRNA.
discontinued: false
dominant: false
experience: experience/None
group u list: _295_
m user id: 0
owner id: 5115
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: NO
star: false
creator: William Vernon
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence annotation: 5nt anti codon
sequence: https://synbiohub.org/public/igem/BBa_K199045_sequence/1

5nt anti codon
type: SequenceAnnotation
persistent identity: annotation2011866
display id: annotation2011866
version: 1
top level: BBa_K199045
location: https://synbiohub.org/public/igem/BBa_K199045/annotation2011866/range2011866/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K199045/annotation2011866/range2011866/1
type: Range
persistent identity: range2011866
display id: range2011866
version: 1
top level: BBa_K199045
direction: direction/forward
start: 46
end: 50
orientation: inline

BBa_K199048
type: ComponentDefinition
persistent identity: BBa K199048
display id: BBa_K199048
version: 1
was generated by: iGEM to SBOL conversion
description: CCACC tRNA Suppressor (Produces Serine)
created: 2009-07-19T11:00:00Z
modified: 2015-05-08T01:11:19Z
mutable provenance: Oligo assembly
owned by: james
owned by: myers
mutable description: A serine tRNA was modified to have the anticodon loop 5' UUGGUGGAA 3'so that the anticodon GGUGG could be used to decode a frameshift mutation of CCACC in a mRNA.
discontinued: false
dominant: false
experience: experience/None
group u list: _295_
m user id: 0
owner id: 5115
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: no
star: false
creator: William Vernon
type: DnaRegion
role: partType/RNA
role: SO:0000834
sequence annotation: 5nt anti codon
sequence: https://synbiohub.org/public/igem/BBa_K199048_sequence/1

5nt anti codon
type: SequenceAnnotation
persistent identity: annotation2011945
display id: annotation2011945
version: 1
top level: BBa_K199048
location: https://synbiohub.org/public/igem/BBa_K199048/annotation2011945/range2011945/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K199048/annotation2011945/range2011945/1
type: Range
persistent identity: range2011945
display id: range2011945
version: 1
top level: BBa_K199048
direction: direction/forward
start: 46
end: 50
orientation: inline

https://synbiohub.org/public/igem/BBa_J119077_sequence/1
type: Sequence
persistent identity: BBa J119077 sequence
display id: BBa_J119077_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagggatccaattcggagagatgccggagcggctgaacggaccggtttggtggaaaccggagtaggggcaactctaccgggggttcaaatccccctctctccgccactgcatatccttagcgaaagctaaggattttttttaagcttactagagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagggatccaattcggagagatgccggagcggctgaacggaccggtctgtcctaaaccggagtaggggcaactctaccgggggttcaaatccccctctctccgccactgcatatccttagcgaaagctaaggattttttttaagcttactagagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagggatccaattcggagagatgccggagcggctgaacggaccggtgtggtagaaaccggagtaggggcaactctaccgggggttcaaatccccctctctccgccactgcatatccttagcgaaagctaaggattttttttaagcttactagagcgttgacaccatcgaatggcgcaaaacctttcgcggtatggcatgatagcgcccggcgtctcggcgaattaaagaggagaaagtaggcatgaacacgagagaccacgcgatcgcgccaccgctaccaggacgctaccaggcgcgccaggtctctgctggaagacgtcacgattaacatcgctaagaacgacttctctgacatcgaactggctgctatcccgttcaacactctagctgaccattacggtgagcgtttagctcgcgaacagttggcccttgagcatgagtcttacgagatgggtgaagcacgcttccgcaagatgtttgagcgtcaacttaaagctggtgaggttgcggataacgctgccgccaagcctctcatcactaccctactccctaagatgattgcacgcatcaacgactggtttgaggaagtgaaagctaagcgcggcaagcgcccgacagccttccagttcctgcaagaaatcaagccggaagccgtagcgtacatcaccattaagaccactctagcttgcctaaccagtgctgacaatacaaccgttcaggctgtagcaagcgcaatcggtcgggccattgaggacgaggctcgcttcggtcgtatccgtgaccttgaagctaagcacttcaagaaaaacgtcgaggaacaactcaacaagcgcgtagggcacgtctacaagaaagcatttatgcaagttgtcgaggctgacatgctctctaagggtctactcggtggcgaggcgtggtcctcgtggcataaggaggactctattcatgtaggagtacgctgcatcgagatgctcattgagtcaaccggaatggttagcttacaccgccaaaatgctggcgtagtaggtcaagactctgagactatcgaactcgcacctgaatacgctgaggctatc…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J119058_sequence/1
type: Sequence
persistent identity: BBa J119058 sequence
display id: BBa_J119058_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cgttgacaccatcgaatggcgcaaaacctttcgcggtatggcatgatagcgcccggcgtctcggcgaattaaagaggagaaagtaggcatgaacacgagagaccacgcgatcgcgccaccgctaccaggacgctaccaggcgcgccaggtctctgctggaagacgtcacgattaacatcgctaagaacgacttctctgacatcgaactggctgctatcccgttcaacactctagctgaccattacggtgagcgtttagctcgcgaacagttggcccttgagcatgagtcttacgagatgggtgaagcacgcttccgcaagatgtttgagcgtcaacttaaagctggtgaggttgcggataacgctgccgccaagcctctcatcactaccctactccctaagatgattgcacgcatcaacgactggtttgaggaagtgaaagctaagcgcggcaagcgcccgacagccttccagttcctgcaagaaatcaagccggaagccgtagcgtacatcaccattaagaccactctagcttgcctaaccagtgctgacaatacaaccgttcaggctgtagcaagcgcaatcggtcgggccattgaggacgaggctcgcttcggtcgtatccgtgaccttgaagctaagcacttcaagaaaaacgtcgaggaacaactcaacaagcgcgtagggcacgtctacaagaaagcatttatgcaagttgtcgaggctgacatgctctctaagggtctactcggtggcgaggcgtggtcctcgtggcataaggaggactctattcatgtaggagtacgctgcatcgagatgctcattgagtcaaccggaatggttagcttacaccgccaaaatgctggcgtagtaggtcaagactctgagactatcgaactcgcacctgaatacgctgaggctatcgcaacccgtgcaggggcgctggctggcatctctccgatgttccaaccttgcgtagttcctcctaagccgtggactggcattactggtggtggctattgggctaacggtcgtcgtcctctagcgctggtgcgtactcacagtaagaaagcactgatgcgctacgaggacgtttacatgcctgaggtgtacaaagcgattaacattgcgcaaaacaccgcatggaaaatcaacaagaaagtcctagcggtcgccaacgtaatcaccaagtggaagcattgtccggtcgaggacatccctgcgattgagcgtgaagaactcccgatgaaaccggaggacatcgacatgaatcctgaggctctcaccgcgtggaaacgtgctgccgctgctgtgtaccgcaaggacaaggctcgcaagtctcgccgtatcagccttgagttcatgcttgagcaagccaataagtttgctaaccataaggccatctggttcccttacaacatggactggcgcggtcgtgtttacgctgtgtcaatgttcaacccgcaaggtaacgatatgaccaaaggactgcttacgctggcgaaaggtaaaccaatcggtaaggaaggttactactggctgaaaatccacggtgcaaactgtgcgggtgtcgataaggttccgttccctgagcgcatcaagttcattgaggaaaaccacgagaacatcatggcttgcgctaagtctccactggagaacacttggtgggctgagcaagattctccgttctgcttccttgcgttctgctttgagtacgctggggtacagcaccacggcctgagctataactgctcccttccgctggcgtttgacgggtcttgctccggcatccagcacttctccgcgatgctccgagatgaggtaggtggtcgcgcggttaacttgcttcctagtgaaaccgttcaggacatctacgggattgttgctaagaaagtcaacgagattctacaagcagacgcaatcaatgggaccgataacgaagtagttaccgtgaccgatgagaacactggtgaaatctctgagaaagtcaagctgggcact…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K199191_sequence/1
type: Sequence
persistent identity: BBa K199191 sequence
display id: BBa_K199191_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagggatccaattcggagagatgccggagcggctgaacggaccggtttggtggaaaccggagtaggggcaactctaccgggggttcaaatccccctctctccgccactgcatatccttagcgaaagctaaggattttttttaagcttactagagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagggatccaattcggagagatgccggagcggctgaacggaccggtctgtcctaaaccggagtaggggcaactctaccgggggttcaaatccccctctctccgccactgcatatccttagcgaaagctaaggattttttttaagcttactagagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagggatccaattcggagagatgccggagcggctgaacggaccggtgtggtagaaaccggagtaggggcaactctaccgggggttcaaatccccctctctccgccactgcatatccttagcgaaagctaaggattttttttaagct
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0010_sequence/1
type: Sequence
persistent identity: BBa R0010 sequence
display id: BBa_R0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K199014_sequence/1
type: Sequence
persistent identity: BBa K199014 sequence
display id: BBa_K199014_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggatccaattcggagagatgccggagcggctgaacggaccggtctgtcctaaaccggagtaggggcaactctaccgggggttcaaatccccctctctccgccactgcatatccttagcgaaagctaaggattttttttaagct
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K199045_sequence/1
type: Sequence
persistent identity: BBa K199045 sequence
display id: BBa_K199045_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggatccaattcggagagatgccggagcggctgaacggaccggtgtggtagaaaccggagtaggggcaactctaccgggggttcaaatccccctctctccgccactgcatatccttagcgaaagctaaggattttttttaagct
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K199048_sequence/1
type: Sequence
persistent identity: BBa K199048 sequence
display id: BBa_K199048_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggatccaattcggagagatgccggagcggctgaacggaccggtttggtggaaaccggagtaggggcaactctaccgggggttcaaatccccctctctccgccactgcatatccttagcgaaagctaaggattttttttaagct
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z