iGEM 2019 Distribution Plate 5 Well 22P
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member: BBa_K1689014

iGEM 2019 Distribution Plate 5 Well 6O
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member: BBa_K1399000

iGEM 2019 Distribution Plate 5 Well 16H
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iGEM 2019 Distribution Plate 5 Well 22H
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member: BBa_K1689006

iGEM 2019 Distribution Plate 5 Well 12F
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member: BBa_K1640012

iGEM 2019 Distribution Plate 5 Well 24G
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member: BBa_K1351021

iGEM 2019 Distribution Plate 5 Well 18E
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member: BBa_K1438002

iGEM 2019 Distribution Plate 5 Well 23E
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member: BBa_K1321162

iGEM 2019 Distribution Plate 5 Well 14F
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member: BBa_K1602008

iGEM 2019 Distribution Plate 5 Well 4K
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member: BBa_K1434000

iGEM 2019 Distribution Plate 5 Well 10N
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member: BBa_K1696015

iGEM 2019 Distribution Plate 5 Well 9O
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iGEM 2019 Distribution Plate 5 Well 14I
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iGEM 2019 Distribution Plate 5 Well 15O
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iGEM 2019 Distribution Plate 5 Well 18J
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iGEM 2019 Distribution Plate 5 Well 13A
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iGEM 2019 Distribution Plate 5 Well 21G
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iGEM 2019 Distribution Plate 5 Well 11F
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member: BBa_K1758325

iGEM 2019 Distribution Plate 5 Well 24B
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member: BBa_K1689015

iGEM 2019 Distribution Plate 5 Well 17L
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iGEM 2019 Distribution Plate 5 Well 7G
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iGEM 2019 Distribution Plate 5 Well 19B
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iGEM 2019 Distribution Plate 5 Well 22N
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member: BBa_K1689013

iGEM 2019 Distribution Plate 5 Well 1A
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member: N-carrier

iGEM 2019 Distribution Plate 5 Well 17H
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member: BBa_K1598003

iGEM 2019 Distribution Plate 5 Well 1B
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member: BBa_K1763000

iGEM 2019 Distribution Plate 5 Well 17B
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member: Art175

iGEM 2019 Distribution Plate 5 Well 24F
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member: BBa_K1689017

iGEM 2019 Distribution Plate 5 Well 11A
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member: BBa_K1315010

iGEM 2019 Distribution Plate 5 Well 8I
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member: BBa_K1399004

iGEM 2019 Distribution Plate 5 Well 21C
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member: BBa_K1321120

iGEM 2019 Distribution Plate 5 Well 19G
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member: BBa_K1321352

iGEM 2019 Distribution Plate 5 Well 12D
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member: BBa_K1640006

iGEM 2019 Distribution Plate 5 Well 7K
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member: BBa_K1362005

iGEM 2019 Distribution Plate 5 Well 17J
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member: BBa_K1604021

iGEM 2019 Distribution Plate 5 Well 8C
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member: BBa_K1399002

iGEM 2019 Distribution Plate 5 Well 4M
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member: BBa_K1434001

iGEM 2019 Distribution Plate 5 Well 6H
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member: BBa_K1763438

iGEM 2019 Distribution Plate 5 Well 7O
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iGEM 2019 Distribution Plate 5 Well 11K
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member: BBa_K1497010

iGEM 2019 Distribution Plate 5 Well 4D
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member: mLacI

iGEM 2019 Distribution Plate 5 Well 20O
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member: Intein

iGEM 2019 Distribution Plate 5 Well 14E
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member: BBa_K1325005

iGEM 2019 Distribution Plate 5 Well 18B
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member: xynA-PDZli

iGEM 2019 Distribution Plate 5 Well 21B
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member: BBa_K1789006

iGEM 2019 Distribution Plate 5 Well 10J
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member: BBa_K1696009

iGEM 2019 Distribution Plate 5 Well 11H
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member: BBa_K1758330

iGEM 2019 Distribution Plate 5 Well 17F
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member: BBa_K1598001

iGEM 2019 Distribution Plate 5 Well 1C
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member: BBa_K1362054

iGEM 2019 Distribution Plate 5 Well 9A
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member: Smt3-mDNMT

iGEM 2019 Distribution Plate 5 Well 20N
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member: BBa_K1825005

iGEM 2019 Distribution Plate 5 Well 8E
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member: BBa_K1399003

iGEM 2019 Distribution Plate 5 Well 15K
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member: BBa_K1321337

iGEM 2019 Distribution Plate 5 Well 5N
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member: BBa_K1585312

iGEM 2019 Distribution Plate 5 Well 18C
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member: BFRM52H

iGEM 2019 Distribution Plate 5 Well 3J
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member: BBa_K1699002

iGEM 2019 Distribution Plate 5 Well 12E
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member: BBa_K1343014

iGEM 2019 Distribution Plate 5 Well 19I
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member: BBa_K1321353

iGEM 2019 Distribution Plate 5 Well 20M
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member: aGal

iGEM 2019 Distribution Plate 5 Well 1E
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member: BBa_K1362055

iGEM 2019 Distribution Plate 5 Well 10L
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member: BBa_K1696013

iGEM 2019 Distribution Plate 5 Well 10G
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member: BBa_K1399013

iGEM 2019 Distribution Plate 5 Well 11D
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member: BBa_K1758323

iGEM 2019 Distribution Plate 5 Well 11M
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member: BBa_K1497011

iGEM 2019 Distribution Plate 5 Well 16E
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member: BBa_K1393001

iGEM 2019 Distribution Plate 5 Well 15I
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version: 1
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member: BBa_K1321009

iGEM 2019 Distribution Plate 5 Well 18K
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member: BBa_K1472610

iGEM 2019 Distribution Plate 5 Well 13N
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member: BBa_K1583100

iGEM 2019 Distribution Plate 5 Well 22F
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member: BBa_K1689005

iGEM 2019 Distribution Plate 5 Well 24L
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member: Mcfp3

iGEM 2019 Distribution Plate 5 Well 21E
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persistent identity: iGEM 2019 Plate5 Well21E
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version: 1
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member: BBa_K1321121

iGEM 2019 Distribution Plate 5 Well 13D
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version: 1
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member: BBa_K1758360

iGEM 2019 Distribution Plate 5 Well 19D
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persistent identity: iGEM 2019 Plate5 Well19D
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version: 1
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member: BBa_K1632013

iGEM 2019 Distribution Plate 5 Well 24D
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persistent identity: iGEM 2019 Plate5 Well24D
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version: 1
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member: BBa_K1689016

iGEM 2019 Distribution Plate 5 Well 14H
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persistent identity: iGEM 2019 Plate5 Well14H
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version: 1
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member: BBa_K1602009

iGEM 2019 Distribution Plate 5 Well 16G
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version: 1
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member: BBa_K1460004

iGEM 2019 Distribution Plate 5 Well 15M
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version: 1
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member: BBa_K1321338

iGEM 2019 Distribution Plate 5 Well 11J
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version: 1
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member: BBa_K1758331

iGEM 2019 Distribution Plate 5 Well 9G
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member: BBa_K1315005

iGEM 2019 Distribution Plate 5 Well 15J
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member: DsbDspBx

iGEM 2019 Distribution Plate 5 Well 1G
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member: BBa_K1362056

iGEM 2019 Distribution Plate 5 Well 20K
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member: EABase

iGEM 2019 Distribution Plate 5 Well 15P
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persistent identity: iGEM 2019 Plate5 Well15P
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member: Fla-Art175

iGEM 2019 Distribution Plate 5 Well 4O
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persistent identity: iGEM 2019 Plate5 Well4O
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member: BBa_K1445000

iGEM 2019 Distribution Plate 5 Well 4H
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version: 1
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member: T7 express

iGEM 2019 Distribution Plate 5 Well 14C
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persistent identity: iGEM 2019 Plate5 Well14C
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version: 1
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member: BBa_K1325002

iGEM 2019 Distribution Plate 5 Well 19F
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persistent identity: iGEM 2019 Plate5 Well19F
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version: 1
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member: BBa_K1632018

iGEM 2019 Distribution Plate 5 Well 3N
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persistent identity: iGEM 2019 Plate5 Well3N
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version: 1
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member: BBa_K1699004

iGEM 2019 Distribution Plate 5 Well 7J
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persistent identity: iGEM 2019 Plate5 Well7J
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version: 1
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member: BBa_K1582029

iGEM 2019 Distribution Plate 5 Well 9B
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persistent identity: iGEM 2019 Plate5 Well9B
display id: iGEM_2019_Plate5_Well9B
version: 1
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member: BBa_K1758120

iGEM 2019 Distribution Plate 5 Well 18O
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persistent identity: iGEM 2019 Plate5 Well18O
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version: 1
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member: BBa_K1523022

iGEM 2019 Distribution Plate 5 Well 24O
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persistent identity: iGEM 2019 Plate5 Well24O
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version: 1
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member: BBa_J04450

iGEM 2019 Distribution Plate 5 Well 23L
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persistent identity: iGEM 2019 Plate5 Well23L
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version: 1
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member: BBa_K1806003

iGEM 2019 Distribution Plate 5 Well 16L
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member: RBS-Scaffo

iGEM 2019 Distribution Plate 5 Well 8K
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persistent identity: iGEM 2019 Plate5 Well8K
display id: iGEM_2019_Plate5_Well8K
version: 1
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member: BBa_K1399006

iGEM 2019 Distribution Plate 5 Well 15H
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persistent identity: iGEM 2019 Plate5 Well15H
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version: 1
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member: MccS

iGEM 2019 Distribution Plate 5 Well 12K
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persistent identity: iGEM 2019 Plate5 Well12K
display id: iGEM_2019_Plate5_Well12K
version: 1
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member: BBa_K1334000

iGEM 2019 Distribution Plate 5 Well 22C
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persistent identity: iGEM 2019 Plate5 Well22C
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version: 1
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member: BBa_K1343019

iGEM 2019 Distribution Plate 5 Well 23F
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persistent identity: iGEM 2019 Plate5 Well23F
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version: 1
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member: BBa_K1632010

iGEM 2019 Distribution Plate 5 Well 6I
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persistent identity: iGEM 2019 Plate5 Well6I
display id: iGEM_2019_Plate5_Well6I
version: 1
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member: BBa_K1423006

iGEM 2019 Distribution Plate 5 Well 7E
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persistent identity: iGEM 2019 Plate5 Well7E
display id: iGEM_2019_Plate5_Well7E
version: 1
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member: SspDnaB(C)

iGEM 2019 Distribution Plate 5 Well 7F
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persistent identity: iGEM 2019 Plate5 Well7F
display id: iGEM_2019_Plate5_Well7F
version: 1
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member: tFba1

iGEM 2019 Distribution Plate 5 Well 20I
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persistent identity: iGEM 2019 Plate5 Well20I
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member: NAGA

iGEM 2019 Distribution Plate 5 Well 3P
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persistent identity: iGEM 2019 Plate5 Well3P
display id: iGEM_2019_Plate5_Well3P
version: 1
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member: BBa_K1699005

iGEM 2019 Distribution Plate 5 Well 6G
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persistent identity: iGEM 2019 Plate5 Well6G
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member: BBa_K1467204

iGEM 2019 Distribution Plate 5 Well 18P
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persistent identity: iGEM 2019 Plate5 Well18P
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version: 1
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member: BBa_K1602055

iGEM 2019 Distribution Plate 5 Well 6A
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persistent identity: iGEM 2019 Plate5 Well6A
display id: iGEM_2019_Plate5_Well6A
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member: BBa_K1478000

iGEM 2019 Distribution Plate 5 Well 18N
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persistent identity: iGEM 2019 Plate5 Well18N
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member: BBa_K1602051

iGEM 2019 Distribution Plate 5 Well 9I
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persistent identity: iGEM 2019 Plate5 Well9I
display id: iGEM_2019_Plate5_Well9I
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member: BBa_K1315006

iGEM 2019 Distribution Plate 5 Well 3L
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persistent identity: iGEM 2019 Plate5 Well3L
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version: 1
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member: BBa_K1699003

iGEM 2019 Distribution Plate 5 Well 4F
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persistent identity: iGEM 2019 Plate5 Well4F
display id: iGEM_2019_Plate5_Well4F
version: 1
owned by: myers
member: mLacI

iGEM 2019 Distribution Plate 5 Well 18M
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persistent identity: iGEM 2019 Plate5 Well18M
display id: iGEM_2019_Plate5_Well18M
version: 1
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member: pBad/araC

iGEM 2019 Distribution Plate 5 Well 15D
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persistent identity: iGEM 2019 Plate5 Well15D
display id: iGEM_2019_Plate5_Well15D
version: 1
owned by: myers
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iGEM 2019 Distribution Plate 5 Well 1P
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iGEM 2019 Distribution Plate 5 Well 13K
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iGEM 2019 Distribution Plate 5 Well 3B
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iGEM 2019 Distribution Plate 5 Well 14G
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iGEM 2019 Distribution Plate 5 Well 8D
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iGEM 2019 Distribution Plate 5 Well 24P
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iGEM 2019 Distribution Plate 5 Well 11G
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iGEM 2019 Distribution Plate 5 Well 7D
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iGEM 2019 Distribution Plate 5 Well 6F
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iGEM 2019 Distribution Plate 5 Well 16N
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iGEM 2019 Distribution Plate 5 Well 17E
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iGEM 2019 Distribution Plate 5 Well 5C
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iGEM 2019 Distribution Plate 5 Well 15N
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iGEM 2019 Distribution Plate 5 Well 16J
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iGEM 2019 Distribution Plate 5 Well 12C
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iGEM 2019 Distribution Plate 5 Well 9K
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iGEM 2019 Distribution Plate 5 Well 2A
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iGEM 2019 Distribution Plate 5 Well 2P
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iGEM 2019 Distribution Plate 5 Well 11B
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iGEM 2019 Distribution Plate 5 Well 11O
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iGEM 2019 Distribution Plate 5 Well 20G
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iGEM 2019 Distribution Plate 5 Well 21P
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iGEM 2019 Distribution Plate 5 Well 14K
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iGEM 2019 Distribution Plate 5 Well 5E
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iGEM 2019 Distribution Plate 5 Well 19C
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iGEM 2019 Distribution Plate 5 Well 4B
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iGEM 2019 Distribution Plate 5 Well 7H
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iGEM 2019 Distribution Plate 5 Well 15L
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iGEM 2019 Distribution Plate 5 Well 5J
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iGEM 2019 Distribution Plate 5 Well 9L
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iGEM 2019 Distribution Plate 5 Well 13M
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iGEM 2019 Distribution Plate 5 Well 9P
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iGEM 2019 Distribution Plate 5 Well 5G
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iGEM 2019 Distribution Plate 5 Well 6C
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iGEM 2019 Distribution Plate 5 Well 11C
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iGEM 2019 Distribution Plate 5 Well 10K
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iGEM 2019 Distribution Plate 5 Well 21F
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iGEM 2019 Distribution Plate 5 Well 1K
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iGEM 2019 Distribution Plate 5 Well 13L
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iGEM 2019 Distribution Plate 5 Well 18G
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iGEM 2019 Distribution Plate 5 Well 3K
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iGEM 2019 Distribution Plate 5 Well 11I
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iGEM 2019 Distribution Plate 5 Well 17A
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iGEM 2019 Distribution Plate 5 Well 9M
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iGEM 2019 Distribution Plate 5 Well 8M
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iGEM 2019 Distribution Plate 5 Well 21N
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iGEM 2019 Distribution Plate 5 Well 3I
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iGEM 2019 Distribution Plate 5 Well 11E
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iGEM 2019 Distribution Plate 5 Well 23G
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iGEM 2019 Distribution Plate 5 Well 22O
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iGEM 2019 Distribution Plate 5 Well 23O
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iGEM 2019 Distribution Plate 5 Well 15G
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iGEM 2019 Distribution Plate 5 Well 16K
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iGEM 2019 Distribution Plate 5 Well 1I
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iGEM 2019 Distribution Plate 5 Well 7I
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iGEM 2019 Distribution Plate 5 Well 7B
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iGEM 2019 Distribution Plate 5 Well 19E
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iGEM 2019 Distribution Plate 5 Well 16D
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iGEM 2019 Distribution Plate 5 Well 3O
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iGEM 2019 Distribution Plate 5 Well 21D
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iGEM 2019 Distribution Plate 5 Well 1H
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iGEM 2019 Distribution Plate 5 Well 22K
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iGEM 2019 Distribution Plate 5 Well 17C
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iGEM 2019 Distribution Plate 5 Well 3M
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iGEM 2019 Distribution Plate 5 Well 14O
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iGEM 2019 Distribution Plate 5 Well 13J
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iGEM 2019 Distribution Plate 5 Well 12A
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iGEM 2019 Distribution Plate 5 Well 10D
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iGEM 2019 Distribution Plate 5 Well 18H
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iGEM 2019 Distribution Plate 5 Well 9E
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iGEM 2019 Distribution Plate 5 Well 19A
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iGEM 2019 Distribution Plate 5 Well 3H
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iGEM 2019 Distribution Plate 5 Well 4N
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iGEM 2019 Distribution Plate 5 Well 1F
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iGEM 2019 Distribution Plate 5 Well 15C
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iGEM 2019 Distribution Plate 5 Well 19L
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iGEM 2019 Distribution Plate 5 Well 2H
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iGEM 2019 Distribution Plate 5 Well 22B
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iGEM 2019 Distribution Plate 5 Well 1N
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iGEM 2019 Distribution Plate 5 Well 19M
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iGEM 2019 Distribution Plate 5 Well 13G
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iGEM 2019 Distribution Plate 5 Well 24E
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iGEM 2019 Distribution Plate 5 Well 3E
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iGEM 2019 Distribution Plate 5 Well 22M
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iGEM 2019 Distribution Plate 5 Well 6P
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iGEM 2019 Distribution Plate 5 Well 14D
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iGEM 2019 Distribution Plate 5 Well 16A
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iGEM 2019 Distribution Plate 5 Well 7C
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iGEM 2019 Distribution Plate 5 Well 22G
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iGEM 2019 Distribution Plate 5 Well 2G
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iGEM 2019 Distribution Plate 5 Well 8H
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iGEM 2019 Distribution Plate 5 Well 14P
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iGEM 2019 Distribution Plate 5 Well 20P
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iGEM 2019 Distribution Plate 5 Well 13E
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iGEM 2019 Distribution Plate 5 Well 18F
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iGEM 2019 Distribution Plate 5 Well 17M
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iGEM 2019 Distribution Plate 5 Well 2F
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iGEM 2019 Distribution Plate 5 Well 20C
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iGEM 2019 Distribution Plate 5 Well 8B
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iGEM 2019 Distribution Plate 5 Well 23C
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iGEM 2019 Distribution Plate 5 Well 4L
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iGEM 2019 Distribution Plate 5 Well 17P
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iGEM 2019 Distribution Plate 5 Well 2J
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iGEM 2019 Distribution Plate 5 Well 9D
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iGEM 2019 Distribution Plate 5 Well 19O
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iGEM 2019 Distribution Plate 5 Well 14B
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iGEM 2019 Distribution Plate 5 Well 13B
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iGEM 2019 Distribution Plate 5 Well 16B
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iGEM 2019 Distribution Plate 5 Well 20A
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iGEM 2019 Distribution Plate 5 Well 19J
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iGEM 2019 Distribution Plate 5 Well 17K
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iGEM 2019 Distribution Plate 5 Well 1M
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iGEM 2019 Distribution Plate 5 Well 22I
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iGEM 2019 Distribution Plate 5 Well 8F
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iGEM 2019 Distribution Plate 5 Well 8N
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iGEM 2019 Distribution Plate 5 Well 10M
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iGEM 2019 Distribution Plate 5 Well 13H
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iGEM 2019 Distribution Plate 5 Well 17D
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iGEM 2019 Distribution Plate 5 Well 16I
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iGEM 2019 Distribution Plate 5 Well 11P
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iGEM 2019 Distribution Plate 5 Well 7L
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iGEM 2019 Distribution Plate 5 Well 14N
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iGEM 2019 Distribution Plate 5 Well 5H
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iGEM 2019 Distribution Plate 5 Well 18D
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iGEM 2019 Distribution Plate 5 Well 9N
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iGEM 2019 Distribution Plate 5 Well 3G
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iGEM 2019 Distribution Plate 5 Well 8O
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iGEM 2019 Distribution Plate 5 Well 5D
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iGEM 2019 Distribution Plate 5 Well 2L
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iGEM 2019 Distribution Plate 5 Well 12O
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iGEM 2019 Distribution Plate 5 Well 21H
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iGEM 2019 Distribution Plate 5 Well 3A
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iGEM 2019 Distribution Plate 5 Well 19P
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iGEM 2019 Distribution Plate 5 Well 4G
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iGEM 2019 Distribution Plate 5 Well 21M
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iGEM 2019 Distribution Plate 5 Well 23P
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iGEM 2019 Distribution Plate 5 Well 2O
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iGEM 2019 Distribution Plate 5 Well 17I
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iGEM 2019 Distribution Plate 5 Well 21I
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iGEM 2019 Distribution Plate 5 Well 12I
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iGEM 2019 Distribution Plate 5 Well 10F
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iGEM 2019 Distribution Plate 5 Well 1O
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iGEM 2019 Distribution Plate 5 Well 6E
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iGEM 2019 Distribution Plate 5 Well 12B
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iGEM 2019 Distribution Plate 5 Well 2C
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iGEM 2019 Distribution Plate 5 Well 9C
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iGEM 2019 Distribution Plate 5 Well 23D
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iGEM 2019 Distribution Plate 5 Well 4A
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iGEM 2019 Distribution Plate 5 Well 6L
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iGEM 2019 Distribution Plate 5 Well 1J
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iGEM 2019 Distribution Plate 5 Well 20J
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iGEM 2019 Distribution Plate 5 Well 24I
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iGEM 2019 Distribution Plate 5 Well 3D
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iGEM 2019 Distribution Plate 5 Well 15E
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iGEM 2019 Distribution Plate 5 Well 18A
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iGEM 2019 Distribution Plate 5 Well 3F
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iGEM 2019 Distribution Plate 5 Well 2N
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iGEM 2019 Distribution Plate 5 Well 1L
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iGEM 2019 Distribution Plate 5 Well 4I
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iGEM 2019 Distribution Plate 5
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iGEM 2019 Distribution Plate 5 Well 4P
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iGEM 2019 Distribution Plate 5 Well 23J
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iGEM 2019 Distribution Plate 5 Well 5F
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iGEM 2019 Distribution Plate 5 Well 15A
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iGEM 2019 Distribution Plate 5 Well 23B
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iGEM 2019 Distribution Plate 5 Well 19K
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iGEM 2019 Distribution Plate 5 Well 21K
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iGEM 2019 Distribution Plate 5 Well 21O
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iGEM 2019 Distribution Plate 5 Well 19N
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iGEM 2019 Distribution Plate 5 Well 16M
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iGEM 2019 Distribution Plate 5 Well 23H
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iGEM 2019 Distribution Plate 5 Well 16O
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iGEM 2019 Distribution Plate 5 Well 7P
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iGEM 2019 Distribution Plate 5 Well 6N
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iGEM 2019 Distribution Plate 5 Well 6B
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iGEM 2019 Distribution Plate 5 Well 4E
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iGEM 2019 Distribution Plate 5 Well 24K
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iGEM 2019 Distribution Plate 5 Well 6D
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iGEM 2019 Distribution Plate 5 Well 4C
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iGEM 2019 Distribution Plate 5 Well 18I
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iGEM 2019 Distribution Plate 5 Well 12L
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iGEM 2019 Distribution Plate 5 Well 9H
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iGEM 2019 Distribution Plate 5 Well 23K
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iGEM 2019 Distribution Plate 5 Well 22D
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iGEM 2019 Distribution Plate 5 Well 22E
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iGEM 2019 Distribution Plate 5 Well 13I
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iGEM 2019 Distribution Plate 5 Well 3C
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iGEM 2019 Distribution Plate 5 Well 18L
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iGEM 2019 Distribution Plate 5 Well 21L
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iGEM 2019 Distribution Plate 5 Well 13C
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iGEM 2019 Distribution Plate 5 Well 16C
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iGEM 2019 Distribution Plate 5 Well 20L
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iGEM 2019 Distribution Plate 5 Well 12H
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iGEM 2019 Distribution Plate 5 Well 10B
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iGEM 2019 Distribution Plate 5 Well 9J
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iGEM 2019 Distribution Plate 5 Well 10E
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iGEM 2019 Distribution Plate 5 Well 15B
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iGEM 2019 Distribution Plate 5 Well 17N
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iGEM 2019 Distribution Plate 5 Well 15F
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iGEM 2019 Distribution Plate 5 Well 5O
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iGEM 2019 Distribution Plate 5 Well 12N
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iGEM 2019 Distribution Plate 5 Well 10P
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iGEM 2019 Distribution Plate 5 Well 20F
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iGEM 2019 Distribution Plate 5 Well 14A
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iGEM 2019 Distribution Plate 5 Well 12J
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iGEM 2019 Distribution Plate 5 Well 13F
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iGEM 2019 Distribution Plate 5 Well 2D
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iGEM 2019 Distribution Plate 5 Well 12M
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iGEM 2019 Distribution Plate 5 Well 24J
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version: 1
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member: BBa_K1592000

iGEM 2019 Distribution Plate 5 Well 7M
type: Collection
persistent identity: iGEM 2019 Plate5 Well7M
display id: iGEM_2019_Plate5_Well7M
version: 1
owned by: myers
member: SspDnaB(N)

iGEM 2019 Distribution Plate 5 Well 5P
type: Collection
persistent identity: iGEM 2019 Plate5 Well5P
display id: iGEM_2019_Plate5_Well5P
version: 1
owned by: myers
member: pBar1

iGEM 2019 Distribution Plate 5 Well 10C
type: Collection
persistent identity: iGEM 2019 Plate5 Well10C
display id: iGEM_2019_Plate5_Well10C
version: 1
owned by: myers
member: BBa_K1399011

iGEM 2019 Distribution Plate 5 Well 21J
type: Collection
persistent identity: iGEM 2019 Plate5 Well21J
display id: iGEM_2019_Plate5_Well21J
version: 1
owned by: myers
member: BBa_K1789011

iGEM 2019 Distribution Plate 5 Well 20E
type: Collection
persistent identity: iGEM 2019 Plate5 Well20E
display id: iGEM_2019_Plate5_Well20E
version: 1
owned by: myers
member: BBa_K1349004

iGEM 2019 Distribution Plate 5 Well 20B
type: Collection
persistent identity: iGEM 2019 Plate5 Well20B
display id: iGEM_2019_Plate5_Well20B
version: 1
owned by: myers
member: BBa_K1602056

iGEM 2019 Distribution Plate 5 Well 2M
type: Collection
persistent identity: iGEM 2019 Plate5 Well2M
display id: iGEM_2019_Plate5_Well2M
version: 1
owned by: myers
member: BBa_K1491018

iGEM 2019 Distribution Plate 5 Well 10I
type: Collection
persistent identity: iGEM 2019 Plate5 Well10I
display id: iGEM_2019_Plate5_Well10I
version: 1
owned by: myers
member: BBa_K1445001

iGEM 2019 Distribution Plate 5 Well 16F
type: Collection
persistent identity: iGEM 2019 Plate5 Well16F
display id: iGEM_2019_Plate5_Well16F
version: 1
owned by: myers
member: HIC genera

iGEM 2019 Distribution Plate 5 Well 5B
type: Collection
persistent identity: iGEM 2019 Plate5 Well5B
display id: iGEM_2019_Plate5_Well5B
version: 1
owned by: myers
member: BBa_K1800002

iGEM 2019 Distribution Plate 5 Well 11N
type: Collection
persistent identity: iGEM 2019 Plate5 Well11N
display id: iGEM_2019_Plate5_Well11N
version: 1
owned by: myers
member: BBa_K1758335

iGEM 2019 Distribution Plate 5 Well 16P
type: Collection
persistent identity: iGEM 2019 Plate5 Well16P
display id: iGEM_2019_Plate5_Well16P
version: 1
owned by: myers
member: aes-SH3lig

iGEM 2019 Distribution Plate 5 Well 12P
type: Collection
persistent identity: iGEM 2019 Plate5 Well12P
display id: iGEM_2019_Plate5_Well12P
version: 1
owned by: myers
member: RBS-xylC

iGEM 2019 Distribution Plate 5 Well 7A
type: Collection
persistent identity: iGEM 2019 Plate5 Well7A
display id: iGEM_2019_Plate5_Well7A
version: 1
owned by: myers
member: sfGFP_T65C

iGEM 2019 Distribution Plate 5 Well 10A
type: Collection
persistent identity: iGEM 2019 Plate5 Well10A
display id: iGEM_2019_Plate5_Well10A
version: 1
owned by: myers
member: BBa_K1399010

iGEM 2019 Distribution Plate 5 Well 11L
type: Collection
persistent identity: iGEM 2019 Plate5 Well11L
display id: iGEM_2019_Plate5_Well11L
version: 1
owned by: myers
member: BBa_K1758333

iGEM 2019 Distribution Plate 5 Well 4J
type: Collection
persistent identity: iGEM 2019 Plate5 Well4J
display id: iGEM_2019_Plate5_Well4J
version: 1
owned by: myers
member: BBa_K1763423

iGEM 2019 Distribution Plate 5 Well 10H
type: Collection
persistent identity: iGEM 2019 Plate5 Well10H
display id: iGEM_2019_Plate5_Well10H
version: 1
owned by: myers
member: BBa_K1696005

iGEM 2019 Distribution Plate 5 Well 8L
type: Collection
persistent identity: iGEM 2019 Plate5 Well8L
display id: iGEM_2019_Plate5_Well8L
version: 1
owned by: myers
member: BBa_K1850010

iGEM 2019 Distribution Plate 5 Well 22L
type: Collection
persistent identity: iGEM 2019 Plate5 Well22L
display id: iGEM_2019_Plate5_Well22L
version: 1
owned by: myers
member: BBa_K1689012

iGEM 2019 Distribution Plate 5 Well 1D
type: Collection
persistent identity: iGEM 2019 Plate5 Well1D
display id: iGEM_2019_Plate5_Well1D
version: 1
owned by: myers
member: BBa_K1763001

iGEM 2019 Distribution Plate 5 Well 14L
type: Collection
persistent identity: iGEM 2019 Plate5 Well14L
display id: iGEM_2019_Plate5_Well14L
version: 1
owned by: myers
member: BBa_K1602011

iGEM 2019 Distribution Plate 5 Well 20H
type: Collection
persistent identity: iGEM 2019 Plate5 Well20H
display id: iGEM_2019_Plate5_Well20H
version: 1
owned by: myers
member: eYFP_SH3li

iGEM 2019 Distribution Plate 5 Well 6M
type: Collection
persistent identity: iGEM 2019 Plate5 Well6M
display id: iGEM_2019_Plate5_Well6M
version: 1
owned by: myers
member: BBa_K1365020

iGEM 2019 Distribution Plate 5 Well 8P
type: Collection
persistent identity: iGEM 2019 Plate5 Well8P
display id: iGEM_2019_Plate5_Well8P
version: 1
owned by: myers
member: BBa_K1850013

iGEM 2019 Distribution Plate 5 Well 19H
type: Collection
persistent identity: iGEM 2019 Plate5 Well19H
display id: iGEM_2019_Plate5_Well19H
version: 1
owned by: myers
member: BBa_K1789000

iGEM 2019 Distribution Plate 5 Well 2B
type: Collection
persistent identity: iGEM 2019 Plate5 Well2B
display id: iGEM_2019_Plate5_Well2B
version: 1
owned by: myers
member: BBa_K1806004

iGEM 2019 Distribution Plate 5 Well 20D
type: Collection
persistent identity: iGEM 2019 Plate5 Well20D
display id: iGEM_2019_Plate5_Well20D
version: 1
owned by: myers
member: BBa_K1602058

iGEM 2019 Distribution Plate 5 Well 6J
type: Collection
persistent identity: iGEM 2019 Plate5 Well6J
display id: iGEM_2019_Plate5_Well6J
version: 1
owned by: myers
member: BBa_K1763439

iGEM 2019 Distribution Plate 5 Well 5M
type: Collection
persistent identity: iGEM 2019 Plate5 Well5M
display id: iGEM_2019_Plate5_Well5M
version: 1
owned by: myers
member: BBa_K1362060

iGEM 2019 Distribution Plate 5 Well 5I
type: Collection
persistent identity: iGEM 2019 Plate5 Well5I
display id: iGEM_2019_Plate5_Well5I
version: 1
owned by: myers
member: BBa_K1362204

iGEM 2019 Distribution Plate 5 Well 9F
type: Collection
persistent identity: iGEM 2019 Plate5 Well9F
display id: iGEM_2019_Plate5_Well9F
version: 1
owned by: myers
member: BBa_K1758204

iGEM 2019 Distribution Plate 5 Well 24H
type: Collection
persistent identity: iGEM 2019 Plate5 Well24H
display id: iGEM_2019_Plate5_Well24H
version: 1
owned by: myers
member: BBa_K1689018

iGEM 2019 Distribution Plate 5 Well 5A
type: Collection
persistent identity: iGEM 2019 Plate5 Well5A
display id: iGEM_2019_Plate5_Well5A
version: 1
owned by: myers
member: TerDnaE3(C

iGEM 2019 Distribution Plate 5 Well 24A
type: Collection
persistent identity: iGEM 2019 Plate5 Well24A
display id: iGEM_2019_Plate5_Well24A
version: 1
owned by: myers
member: mRdRP

iGEM 2019 Distribution Plate 5 Well 22J
type: Collection
persistent identity: iGEM 2019 Plate5 Well22J
display id: iGEM_2019_Plate5_Well22J
version: 1
owned by: myers
member: BBa_K1689007

iGEM 2019 Distribution Plate 5 Well 14J
type: Collection
persistent identity: iGEM 2019 Plate5 Well14J
display id: iGEM_2019_Plate5_Well14J
version: 1
owned by: myers
member: BBa_K1602010

iGEM 2019 Distribution Plate 5 Well 23I
type: Collection
persistent identity: iGEM 2019 Plate5 Well23I
display id: iGEM_2019_Plate5_Well23I
version: 1
owned by: myers
member: BBa_K1321163

iGEM 2019 Distribution Plate 5 Well 23A
type: Collection
persistent identity: iGEM 2019 Plate5 Well23A
display id: iGEM_2019_Plate5_Well23A
version: 1
owned by: myers
member: BBa_K1321154

iGEM 2019 Distribution Plate 5 Well 10O
type: Collection
persistent identity: iGEM 2019 Plate5 Well10O
display id: iGEM_2019_Plate5_Well10O
version: 1
owned by: myers
member: BBa_K1331002

iGEM 2019 Distribution Plate 5 Well 21A
type: Collection
persistent identity: iGEM 2019 Plate5 Well21A
display id: iGEM_2019_Plate5_Well21A
version: 1
owned by: myers
member: BBa_K1321333

iGEM 2019 Distribution Plate 5 Well 13O
type: Collection
persistent identity: iGEM 2019 Plate5 Well13O
display id: iGEM_2019_Plate5_Well13O
version: 1
owned by: myers
member: BBa_K1321090

iGEM 2019 Distribution Plate 5 Well 8A
type: Collection
persistent identity: iGEM 2019 Plate5 Well8A
display id: iGEM_2019_Plate5_Well8A
version: 1
owned by: myers
member: BBa_K1399001

iGEM 2019 Distribution Plate 5 Well 22A
type: Collection
persistent identity: iGEM 2019 Plate5 Well22A
display id: iGEM_2019_Plate5_Well22A
version: 1
owned by: myers
member: BBa_K1446002

iGEM 2019 Distribution Plate 5 Well 17O
type: Collection
persistent identity: iGEM 2019 Plate5 Well17O
display id: iGEM_2019_Plate5_Well17O
version: 1
owned by: myers
member: BBa_K1321347

iGEM 2019 Distribution Plate 5 Well 12G
type: Collection
persistent identity: iGEM 2019 Plate5 Well12G
display id: iGEM_2019_Plate5_Well12G
version: 1
owned by: myers
member: ONBY RS

iGEM 2019 Distribution Plate 5 Well 14M
type: Collection
persistent identity: iGEM 2019 Plate5 Well14M
display id: iGEM_2019_Plate5_Well14M
version: 1
owned by: myers
member: BBa_K1424000

iGEM 2019 Distribution Plate 5 Well 8G
type: Collection
persistent identity: iGEM 2019 Plate5 Well8G
display id: iGEM_2019_Plate5_Well8G
version: 1
owned by: myers
member: BBa_K1399005

iGEM 2019 Distribution Plate 5 Well 5K
type: Collection
persistent identity: iGEM 2019 Plate5 Well5K
display id: iGEM_2019_Plate5_Well5K
version: 1
owned by: myers
member: BBa_K1362205

iGEM 2019 Distribution Plate 5 Well 13P
type: Collection
persistent identity: iGEM 2019 Plate5 Well13P
display id: iGEM_2019_Plate5_Well13P
version: 1
owned by: myers
member: BBa_K1659501

iGEM 2019 Distribution Plate 5 Well 2E
type: Collection
persistent identity: iGEM 2019 Plate5 Well2E
display id: iGEM_2019_Plate5_Well2E
version: 1
owned by: myers
member: BBa_K1491009

iGEM 2019 Distribution Plate 5 Well 6K
type: Collection
persistent identity: iGEM 2019 Plate5 Well6K
display id: iGEM_2019_Plate5_Well6K
version: 1
owned by: myers
member: BBa_K1365000

iGEM 2019 Distribution Plate 5 Well 2K
type: Collection
persistent identity: iGEM 2019 Plate5 Well2K
display id: iGEM_2019_Plate5_Well2K
version: 1
owned by: myers
member: BBa_K1491017

iGEM 2019 Distribution Plate 5 Well 8J
type: Collection
persistent identity: iGEM 2019 Plate5 Well8J
display id: iGEM_2019_Plate5_Well8J
version: 1
owned by: myers
member: Tamura

iGEM 2019 Distribution Plate 5 Well 5L
type: Collection
persistent identity: iGEM 2019 Plate5 Well5L
display id: iGEM_2019_Plate5_Well5L
version: 1
owned by: myers
member: BBa_K1585310

BBa_K1758105
type: ComponentDefinition
persistent identity: BBa K1758105
display id: BBa_K1758105
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: mRFP generator under control of T7 promoter
creator: Team Bielefeld-CeBiTec 2015
created: 2015-08-26T11:00:00Z
modified: 2015-09-18T04:41:35Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2180_
m user id: 27935
owner id: 26563
owning group id: 9
status: status/Planning
bookmark: false
mutable description: mRFP is synthesized when T7-polymerase is induced.
mutable notes: ---
mutable provenance: Both subparts were obtained from iGEM distribution plates.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_I719005
component: BBa_K516030
sequence annotation: BBa_I719005
sequence annotation: BBa_K516030
sequence: https://synbiohub.org/public/igem/BBa_K1758105_sequence/1

BBa_I719005
type: Component
persistent identity: component2439070
display id: component2439070
version: 1
top level: BBa_K1758105
access: public
definition: https://synbiohub.org/public/igem/BBa_I719005/1

BBa_K516030
type: Component
persistent identity: component2439084
display id: component2439084
version: 1
top level: BBa_K1758105
access: public
definition: https://synbiohub.org/public/igem/BBa_K516030/1

BBa_I719005
type: SequenceAnnotation
persistent identity: annotation2439070
display id: annotation2439070
version: 1
top level: BBa_K1758105
location: https://synbiohub.org/public/igem/BBa_K1758105/annotation2439070/range2439070/1
component: BBa_I719005

https://synbiohub.org/public/igem/BBa_K1758105/annotation2439070/range2439070/1
type: Range
persistent identity: range2439070
display id: range2439070
version: 1
direction: direction/reverse
top level: BBa_K1758105
start: 1
end: 23
orientation: inline

BBa_K516030
type: SequenceAnnotation
persistent identity: annotation2439084
display id: annotation2439084
version: 1
top level: BBa_K1758105
location: https://synbiohub.org/public/igem/BBa_K1758105/annotation2439084/range2439084/1
component: BBa_K516030

https://synbiohub.org/public/igem/BBa_K1758105/annotation2439084/range2439084/1
type: Range
persistent identity: range2439084
display id: range2439084
version: 1
direction: direction/reverse
top level: BBa_K1758105
start: 32
end: 895
orientation: inline

BBa_K1321200
type: ComponentDefinition
persistent identity: BBa K1321200
display id: BBa_K1321200
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23101+B0034+VHb (Vitreoscilla haemoglobin)
creator: Xenia Spencer-Milnes
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:09:52Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1696_
m user id: 0
owner id: 20780
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: J23101+RBS+VhB. TBC
mutable notes: J23101+RBS+VhB. TBC
mutable provenance: J23101+RBS+VhB. TBC
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: Start
sequence annotation: Stop
sequence annotation: B0034
sequence annotation: J23101 constitutive
sequence annotation: VHb
sequence: https://synbiohub.org/public/igem/BBa_K1321200_sequence/1

Start
type: SequenceAnnotation
persistent identity: annotation2422496
display id: annotation2422496
version: 1
top level: BBa_K1321200
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1321200/annotation2422496/range2422496/1

https://synbiohub.org/public/igem/BBa_K1321200/annotation2422496/range2422496/1
type: Range
persistent identity: range2422496
display id: range2422496
version: 1
direction: direction/forward
top level: BBa_K1321200
start: 64
end: 66
orientation: inline

Stop
type: SequenceAnnotation
persistent identity: annotation2422497
display id: annotation2422497
version: 1
top level: BBa_K1321200
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1321200/annotation2422497/range2422497/1

https://synbiohub.org/public/igem/BBa_K1321200/annotation2422497/range2422497/1
type: Range
persistent identity: range2422497
display id: range2422497
version: 1
direction: direction/forward
top level: BBa_K1321200
start: 502
end: 504
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2422493
display id: annotation2422493
version: 1
top level: BBa_K1321200
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1321200/annotation2422493/range2422493/1

https://synbiohub.org/public/igem/BBa_K1321200/annotation2422493/range2422493/1
type: Range
persistent identity: range2422493
display id: range2422493
version: 1
direction: direction/forward
top level: BBa_K1321200
start: 44
end: 55
orientation: inline

J23101 constitutive
type: SequenceAnnotation
persistent identity: annotation2422494
display id: annotation2422494
version: 1
top level: BBa_K1321200
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1321200/annotation2422494/range2422494/1

https://synbiohub.org/public/igem/BBa_K1321200/annotation2422494/range2422494/1
type: Range
persistent identity: range2422494
display id: range2422494
version: 1
direction: direction/forward
top level: BBa_K1321200
start: 1
end: 35
orientation: inline

VHb
type: SequenceAnnotation
persistent identity: annotation2422498
display id: annotation2422498
version: 1
top level: BBa_K1321200
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1321200/annotation2422498/range2422498/1

https://synbiohub.org/public/igem/BBa_K1321200/annotation2422498/range2422498/1
type: Range
persistent identity: range2422498
display id: range2422498
version: 1
direction: direction/forward
top level: BBa_K1321200
start: 64
end: 504
orientation: inline

BBa_K1438002
type: ComponentDefinition
persistent identity: BBa K1438002
display id: BBa_K1438002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Hu_Ferritin
creator: Johann Bauerfeind
created: 2014-10-05T11:00:00Z
modified: 2015-07-22T02:13:57Z
discontinued: false
dominant: false
group u list: _1816_
m user id: 4206
owner id: 19714
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Human ferritins do loads more iron atoms then bacterial ferritins. Furthermore they are forming metall cores that are less contaminated with phosphates then e.g. bacterial ferritins. Due to this fact, this should result in a purer iron crystal with an improved magnetic moment.
mutable notes: This design was provided by iGEM Calgary and the HuFerritin as a single protein coding biobrick was constructed by iGEM Berlin as this biobrick was not accessible in this version in the registry.
mutable provenance: iGEM Calgary 2013 from a synthesized codon optimized construct
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1438002_sequence/1

BBa_K1321346
type: ComponentDefinition
persistent identity: BBa K1321346
display id: BBa_K1321346
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CBDclos fused to sfGFP in RFC 25
creator: Chris N Micklem
created: 2014-10-07T11:00:00Z
modified: 2015-06-17T12:30:31Z
discontinued: false
dominant: false
group u list: _1696_
m user id: 4206
owner id: 20830
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: asdf
mutable notes: asdf
mutable provenance: asdf
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_B0105
component: BBa_K1321337
component: BBa_K1321002
sequence annotation: BBa_B0105
sequence annotation: BBa_K1321002
sequence annotation: BBa_K1321337
sequence: https://synbiohub.org/public/igem/BBa_K1321346_sequence/1

BBa_B0105
type: Component
persistent identity: component2415123
display id: component2415123
version: 1
top level: BBa_K1321346
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_K1321337
type: Component
persistent identity: component2415125
display id: component2415125
version: 1
top level: BBa_K1321346
access: public
definition: BBa_K1321337

BBa_K1321002
type: Component
persistent identity: component2415121
display id: component2415121
version: 1
top level: BBa_K1321346
access: public
definition: https://synbiohub.org/public/igem/BBa_K1321002/1

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2415123
display id: annotation2415123
version: 1
top level: BBa_K1321346
location: https://synbiohub.org/public/igem/BBa_K1321346/annotation2415123/range2415123/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_K1321346/annotation2415123/range2415123/1
type: Range
persistent identity: range2415123
display id: range2415123
version: 1
direction: direction/reverse
top level: BBa_K1321346
start: 301
end: 306
orientation: inline

BBa_K1321002
type: SequenceAnnotation
persistent identity: annotation2415121
display id: annotation2415121
version: 1
top level: BBa_K1321346
location: https://synbiohub.org/public/igem/BBa_K1321346/annotation2415121/range2415121/1
component: BBa_K1321002

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type: Range
persistent identity: range2415121
display id: range2415121
version: 1
direction: direction/reverse
top level: BBa_K1321346
start: 1
end: 300
orientation: inline

BBa_K1321337
type: SequenceAnnotation
persistent identity: annotation2415125
display id: annotation2415125
version: 1
top level: BBa_K1321346
location: https://synbiohub.org/public/igem/BBa_K1321346/annotation2415125/range2415125/1
component: BBa_K1321337

https://synbiohub.org/public/igem/BBa_K1321346/annotation2415125/range2415125/1
type: Range
persistent identity: range2415125
display id: range2415125
version: 1
direction: direction/reverse
top level: BBa_K1321346
start: 307
end: 1017
orientation: inline

SspDnaB(N*
type: ComponentDefinition
persistent identity: BBa K1362142
display id: BBa_K1362142
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS + SspDnaB N-intein RFC[105] non-splicing control construct (with His6)
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B??scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch??fer, Carolin Schmelas, Silvan Schmitz, Max Waldha
created: 2014-10-08T11:00:00Z
modified: 2015-05-08T01:10:05Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1738_
m user id: 0
owner id: 12377
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: ???
mutable notes: ???
mutable provenance: ???
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1362430
component: BBa_K1362408
component: BBa_K1362437
component: BBa_K1362417
component: BBa_G0000
component: BBa_K1362090
component: BBa_K1362414
component: BBa_K1362421
component: BBa_K1362416
component: BBa_K1362422
sequence annotation: BBa_K1362417
sequence annotation: BBa_G0000
sequence annotation: BBa_K1362414
sequence annotation: BBa_K1362090
sequence annotation: BBa_K1362421
sequence annotation: BBa_K1362422
sequence annotation: BBa_K1362416
sequence annotation: BBa_K1362437
sequence annotation: BBa_K1362430
sequence annotation: BBa_K1362408
sequence: https://synbiohub.org/public/igem/BBa_K1362142_sequence/1

BBa_K1362430
type: Component
persistent identity: component2410649
display id: component2410649
version: 1
top level: SspDnaB(N*
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362430/1

BBa_K1362408
type: Component
persistent identity: component2410643
display id: component2410643
version: 1
top level: SspDnaB(N*
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362408/1

BBa_K1362437
type: Component
persistent identity: component2410653
display id: component2410653
version: 1
top level: SspDnaB(N*
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362437/1

BBa_K1362417
type: Component
persistent identity: component2410646
display id: component2410646
version: 1
top level: SspDnaB(N*
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362417/1

BBa_G0000
type: Component
persistent identity: component2410619
display id: component2410619
version: 1
top level: SspDnaB(N*
access: public
definition: https://synbiohub.org/public/igem/BBa_G0000/1

BBa_K1362090
type: Component
persistent identity: component2410618
display id: component2410618
version: 1
top level: SspDnaB(N*
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362090/1

BBa_K1362414
type: Component
persistent identity: component2410620
display id: component2410620
version: 1
top level: SspDnaB(N*
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362414/1

BBa_K1362421
type: Component
persistent identity: component2410651
display id: component2410651
version: 1
top level: SspDnaB(N*
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362421/1

BBa_K1362416
type: Component
persistent identity: component2410642
display id: component2410642
version: 1
top level: SspDnaB(N*
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362416/1

BBa_K1362422
type: Component
persistent identity: component2410641
display id: component2410641
version: 1
top level: SspDnaB(N*
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362422/1

BBa_K1362417
type: SequenceAnnotation
persistent identity: annotation2410646
display id: annotation2410646
version: 1
top level: SspDnaB(N*
location: https://synbiohub.org/public/igem/BBa_K1362142/annotation2410646/range2410646/1
component: BBa_K1362417

https://synbiohub.org/public/igem/BBa_K1362142/annotation2410646/range2410646/1
type: Range
persistent identity: range2410646
display id: range2410646
version: 1
direction: direction/reverse
top level: SspDnaB(N*
start: 1161
end: 1166
orientation: inline

BBa_G0000
type: SequenceAnnotation
persistent identity: annotation2410619
display id: annotation2410619
version: 1
top level: SspDnaB(N*
location: https://synbiohub.org/public/igem/BBa_K1362142/annotation2410619/range2410619/1
component: BBa_G0000

https://synbiohub.org/public/igem/BBa_K1362142/annotation2410619/range2410619/1
type: Range
persistent identity: range2410619
display id: range2410619
version: 1
direction: direction/reverse
top level: SspDnaB(N*
start: 30
end: 35
orientation: inline

BBa_K1362414
type: SequenceAnnotation
persistent identity: annotation2410620
display id: annotation2410620
version: 1
top level: SspDnaB(N*
location: https://synbiohub.org/public/igem/BBa_K1362142/annotation2410620/range2410620/1
component: BBa_K1362414

https://synbiohub.org/public/igem/BBa_K1362142/annotation2410620/range2410620/1
type: Range
persistent identity: range2410620
display id: range2410620
version: 1
direction: direction/reverse
top level: SspDnaB(N*
start: 36
end: 38
orientation: inline

BBa_K1362090
type: SequenceAnnotation
persistent identity: annotation2410618
display id: annotation2410618
version: 1
top level: SspDnaB(N*
location: https://synbiohub.org/public/igem/BBa_K1362142/annotation2410618/range2410618/1
component: BBa_K1362090

https://synbiohub.org/public/igem/BBa_K1362142/annotation2410618/range2410618/1
type: Range
persistent identity: range2410618
display id: range2410618
version: 1
direction: direction/reverse
top level: SspDnaB(N*
start: 1
end: 29
orientation: inline

BBa_K1362421
type: SequenceAnnotation
persistent identity: annotation2410651
display id: annotation2410651
version: 1
top level: SspDnaB(N*
location: https://synbiohub.org/public/igem/BBa_K1362142/annotation2410651/range2410651/1
component: BBa_K1362421

https://synbiohub.org/public/igem/BBa_K1362142/annotation2410651/range2410651/1
type: Range
persistent identity: range2410651
display id: range2410651
version: 1
direction: direction/reverse
top level: SspDnaB(N*
start: 1191
end: 1194
orientation: inline

BBa_K1362422
type: SequenceAnnotation
persistent identity: annotation2410641
display id: annotation2410641
version: 1
top level: SspDnaB(N*
location: https://synbiohub.org/public/igem/BBa_K1362142/annotation2410641/range2410641/1
component: BBa_K1362422

https://synbiohub.org/public/igem/BBa_K1362142/annotation2410641/range2410641/1
type: Range
persistent identity: range2410641
display id: range2410641
version: 1
direction: direction/reverse
top level: SspDnaB(N*
start: 39
end: 1121
orientation: inline

BBa_K1362416
type: SequenceAnnotation
persistent identity: annotation2410642
display id: annotation2410642
version: 1
top level: SspDnaB(N*
location: https://synbiohub.org/public/igem/BBa_K1362142/annotation2410642/range2410642/1
component: BBa_K1362416

https://synbiohub.org/public/igem/BBa_K1362142/annotation2410642/range2410642/1
type: Range
persistent identity: range2410642
display id: range2410642
version: 1
direction: direction/reverse
top level: SspDnaB(N*
start: 1122
end: 1125
orientation: inline

BBa_K1362437
type: SequenceAnnotation
persistent identity: annotation2410653
display id: annotation2410653
version: 1
top level: SspDnaB(N*
location: https://synbiohub.org/public/igem/BBa_K1362142/annotation2410653/range2410653/1
component: BBa_K1362437

https://synbiohub.org/public/igem/BBa_K1362142/annotation2410653/range2410653/1
type: Range
persistent identity: range2410653
display id: range2410653
version: 1
direction: direction/reverse
top level: SspDnaB(N*
start: 1195
end: 1197
orientation: inline

BBa_K1362430
type: SequenceAnnotation
persistent identity: annotation2410649
display id: annotation2410649
version: 1
top level: SspDnaB(N*
location: https://synbiohub.org/public/igem/BBa_K1362142/annotation2410649/range2410649/1
component: BBa_K1362430

https://synbiohub.org/public/igem/BBa_K1362142/annotation2410649/range2410649/1
type: Range
persistent identity: range2410649
display id: range2410649
version: 1
direction: direction/reverse
top level: SspDnaB(N*
start: 1167
end: 1190
orientation: inline

BBa_K1362408
type: SequenceAnnotation
persistent identity: annotation2410643
display id: annotation2410643
version: 1
top level: SspDnaB(N*
location: https://synbiohub.org/public/igem/BBa_K1362142/annotation2410643/range2410643/1
component: BBa_K1362408

https://synbiohub.org/public/igem/BBa_K1362142/annotation2410643/range2410643/1
type: Range
persistent identity: range2410643
display id: range2410643
version: 1
direction: direction/reverse
top level: SspDnaB(N*
start: 1126
end: 1160
orientation: inline

BBa_K1789001
type: ComponentDefinition
persistent identity: BBa K1789001
display id: BBa_K1789001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: IaaH
creator: Xinyuan Qiu
created: 2015-09-13T11:00:00Z
modified: 2015-09-14T05:49:28Z
discontinued: false
dominant: false
group u list: _2214_
m user id: 21134
owner id: 21134
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This part is the coding sequence of the enzyme Indoleacetimide hydrolase Iaa H. This enzyme catalyses the hydrolysis of indoleacetamide to indoleacetate and ammonia. This part is gathered from the 2-step pathway in Pseudomonas savastanoi producing the auxin, indole-3-acetic acid.
mutable notes: In order to get the whole sequence of IAAH, we removed the RBS and other irrelevant sequences in K515100 when designing the PCR primers.
mutable provenance: This part is gained from the part BBa_K515100 by PCR.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: IaaH
sequence: https://synbiohub.org/public/igem/BBa_K1789001_sequence/1

IaaH
type: SequenceAnnotation
persistent identity: annotation2454035
display id: annotation2454035
version: 1
top level: BBa_K1789001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1789001/annotation2454035/range2454035/1

https://synbiohub.org/public/igem/BBa_K1789001/annotation2454035/range2454035/1
type: Range
persistent identity: range2454035
display id: range2454035
version: 1
direction: direction/forward
top level: BBa_K1789001
start: 1
end: 1335
orientation: inline

BBa_K1332001
type: ComponentDefinition
persistent identity: BBa K1332001
display id: BBa_K1332001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Histidine tag (8 AA) and RFP
creator: Kenta Nomura
created: 2014-09-24T11:00:00Z
modified: 2015-09-17T07:39:06Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1707_
m user id: 20591
owner id: 21011
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part consists of histidine tag and RFP. His-tag is useful for purifying RFP.
mutable notes: None
mutable provenance: BBa_E1010
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1332001_sequence/1

BBa_K1321353
type: ComponentDefinition
persistent identity: BBa K1321353
display id: BBa_K1321353
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NiBP fused to CBDclos driven by T7
creator: Chris N Micklem
created: 2014-10-07T11:00:00Z
modified: 2015-06-17T12:32:03Z
discontinued: false
dominant: false
group u list: _1696_
m user id: 4206
owner id: 20830
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: asdf
mutable notes: asdf
mutable provenance: asdf
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1319106
component: BBa_K1321343
component: BBa_K1321338
sequence annotation: BBa_K1319106
sequence annotation: BBa_K1321338
sequence annotation: BBa_K1321343
sequence: https://synbiohub.org/public/igem/BBa_K1321353_sequence/1

BBa_K1319106
type: Component
persistent identity: component2415405
display id: component2415405
version: 1
top level: BBa_K1321353
access: public
definition: https://synbiohub.org/public/igem/BBa_K1319106/1

BBa_K1321343
type: Component
persistent identity: component2415410
display id: component2415410
version: 1
top level: BBa_K1321353
access: public
definition: BBa_K1321343

BBa_K1321338
type: Component
persistent identity: component2415403
display id: component2415403
version: 1
top level: BBa_K1321353
access: public
definition: BBa_K1321338

BBa_K1319106
type: SequenceAnnotation
persistent identity: annotation2415405
display id: annotation2415405
version: 1
top level: BBa_K1321353
location: https://synbiohub.org/public/igem/BBa_K1321353/annotation2415405/range2415405/1
component: BBa_K1319106

https://synbiohub.org/public/igem/BBa_K1321353/annotation2415405/range2415405/1
type: Range
persistent identity: range2415405
display id: range2415405
version: 1
direction: direction/reverse
top level: BBa_K1321353
start: 44
end: 58
orientation: inline

BBa_K1321338
type: SequenceAnnotation
persistent identity: annotation2415403
display id: annotation2415403
version: 1
top level: BBa_K1321353
location: https://synbiohub.org/public/igem/BBa_K1321353/annotation2415403/range2415403/1
component: BBa_K1321338

https://synbiohub.org/public/igem/BBa_K1321353/annotation2415403/range2415403/1
type: Range
persistent identity: range2415403
display id: range2415403
version: 1
direction: direction/reverse
top level: BBa_K1321353
start: 1
end: 43
orientation: inline

BBa_K1321343
type: SequenceAnnotation
persistent identity: annotation2415410
display id: annotation2415410
version: 1
top level: BBa_K1321353
location: https://synbiohub.org/public/igem/BBa_K1321353/annotation2415410/range2415410/1
component: BBa_K1321343

https://synbiohub.org/public/igem/BBa_K1321353/annotation2415410/range2415410/1
type: Range
persistent identity: range2415410
display id: range2415410
version: 1
direction: direction/reverse
top level: BBa_K1321353
start: 59
end: 540
orientation: inline

BBa_K1321351
type: ComponentDefinition
persistent identity: BBa K1321351
display id: BBa_K1321351
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CBDcenA with linker fused to NiBP in Freiburg format (RFC 25)
creator: Chris N Micklem
created: 2014-10-07T11:00:00Z
modified: 2015-06-17T12:31:45Z
discontinued: false
dominant: false
group u list: _1696_
m user id: 4206
owner id: 20830
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: asdf
mutable notes: asdf
mutable provenance: asdf
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_B0105
component: BBa_K1321009
component: BBa_K1321339
sequence annotation: BBa_B0105
sequence annotation: BBa_K1321009
sequence annotation: BBa_K1321339
sequence: https://synbiohub.org/public/igem/BBa_K1321351_sequence/1

BBa_B0105
type: Component
persistent identity: component2430309
display id: component2430309
version: 1
top level: BBa_K1321351
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_K1321009
type: Component
persistent identity: component2430311
display id: component2430311
version: 1
top level: BBa_K1321351
access: public
definition: BBa_K1321009

BBa_K1321339
type: Component
persistent identity: component2430307
display id: component2430307
version: 1
top level: BBa_K1321351
access: public
definition: BBa_K1321339

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2430309
display id: annotation2430309
version: 1
top level: BBa_K1321351
location: https://synbiohub.org/public/igem/BBa_K1321351/annotation2430309/range2430309/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_K1321351/annotation2430309/range2430309/1
type: Range
persistent identity: range2430309
display id: range2430309
version: 1
direction: direction/reverse
top level: BBa_K1321351
start: 409
end: 414
orientation: inline

BBa_K1321009
type: SequenceAnnotation
persistent identity: annotation2430311
display id: annotation2430311
version: 1
top level: BBa_K1321351
location: https://synbiohub.org/public/igem/BBa_K1321351/annotation2430311/range2430311/1
component: BBa_K1321009

https://synbiohub.org/public/igem/BBa_K1321351/annotation2430311/range2430311/1
type: Range
persistent identity: range2430311
display id: range2430311
version: 1
direction: direction/reverse
top level: BBa_K1321351
start: 415
end: 590
orientation: inline

BBa_K1321339
type: SequenceAnnotation
persistent identity: annotation2430307
display id: annotation2430307
version: 1
top level: BBa_K1321351
location: https://synbiohub.org/public/igem/BBa_K1321351/annotation2430307/range2430307/1
component: BBa_K1321339

https://synbiohub.org/public/igem/BBa_K1321351/annotation2430307/range2430307/1
type: Range
persistent identity: range2430307
display id: range2430307
version: 1
direction: direction/reverse
top level: BBa_K1321351
start: 1
end: 408
orientation: inline

BBa_K1343020
type: ComponentDefinition
persistent identity: BBa K1343020
display id: BBa_K1343020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Plux->Green Renilla Luciferase->lacI
creator: Tal Naor, Ricarina Rabinovitz, Ittai Rubinstein, Yair Maimon
created: 2014-10-05T11:00:00Z
modified: 2015-05-08T01:10:00Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1718_
m user id: 0
owner id: 20669
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: The promoter Plux is induced by the binding of an AHL-luxR dimer. This bio-brick leads to the synthesis of the enzyme Green Renilla Luciferase, which catalyses a luminescent reaction. lacI can be used to repress the promoter Plac.
mutable notes: r
mutable provenance: BBa_R0062: Plux, BBa_B0030: Strong RBS, BBa_B0030: Strong RBS, BBa_B0010:T1 (terminator) from E. coli.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K1343020_sequence/1

BBa_K1602012
type: ComponentDefinition
persistent identity: BBa K1602012
display id: BBa_K1602012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: yjhG
creator: Leon Kraus, Jonathan Pletzer-Zelgert, Jonas Sindlinger, Carmen Sakas Gandullo, Sebastian Holzner, Elena Tonchevska, Mirjam Haun
created: 2015-09-13T11:00:00Z
modified: 2015-09-18T06:39:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2019_
m user id: 26220
owner id: 25878
owning group id: 9
status: status/Planning
bookmark: false
mutable description: BLANK
mutable notes: BLANK
mutable provenance: BLANK
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1602012_sequence/1

EABase
type: ComponentDefinition
persistent identity: BBa K1483001
display id: BBa_K1483001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Endo-β-Galactosidase
creator: Philipp H. O. Mayer and Philip Roessler
created: 2014-10-05T11:00:00Z
modified: 2015-05-08T01:10:41Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1863_
m user id: 0
owner id: 21259
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Encodes for an enzyme, that is capable of cleaving A- and B-antigens in the human ABO-Bloodgroup system. As the name suggests, the galactose and connected saccharide residues are cleaved from the antigen, leaving behind the the Oh-Antigen which is characteristic of the Bombay-blood type. Part in RFC25.
mutable notes: Part in RFC25, in order to ease fusion with other parts, for example immobilisation tags.
mutable provenance: The protein encoded by this sequence naturally occurs in Clostridium perfringens. This sequence was optimized for codon usage in E. coli K12 and synthesized.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Endo-Beta-Galactosidase
sequence: https://synbiohub.org/public/igem/BBa_K1483001_sequence/1

Endo-Beta-Galactosidase
type: SequenceAnnotation
persistent identity: annotation2396228
display id: annotation2396228
version: 1
top level: EABase
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1483001/annotation2396228/range2396228/1

https://synbiohub.org/public/igem/BBa_K1483001/annotation2396228/range2396228/1
type: Range
persistent identity: range2396228
display id: range2396228
version: 1
direction: direction/forward
top level: EABase
start: 1
end: 2397
orientation: inline

BBa_K1321337
type: ComponentDefinition
persistent identity: BBa K1321337
display id: BBa_K1321337
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: sfGFP in Freiburg format (RFC 25)
creator: Chris N Micklem
created: 2014-10-07T11:00:00Z
modified: 2016-01-26T02:10:16Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 4206
owner id: 20830
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: sfGFP in Freiburg format (RFC 25) to allow for easy use in fusion proteins.
mutable notes: OEPCR
mutable provenance: Made from existing part.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: sfGFP
sequence: https://synbiohub.org/public/igem/BBa_K1321337_sequence/1

sfGFP
type: SequenceAnnotation
persistent identity: annotation2417826
display id: annotation2417826
version: 1
top level: BBa_K1321337
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1321337/annotation2417826/range2417826/1

https://synbiohub.org/public/igem/BBa_K1321337/annotation2417826/range2417826/1
type: Range
persistent identity: range2417826
display id: range2417826
version: 1
direction: direction/forward
top level: BBa_K1321337
start: 1
end: 711
orientation: inline

BBa_K1321333
type: ComponentDefinition
persistent identity: BBa K1321333
display id: BBa_K1321333
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSB-AraC-pBAD
creator: Laura de Arroyo Garcia
created: 2014-10-07T11:00:00Z
modified: 2015-06-17T12:27:41Z
discontinued: false
dominant: false
group u list: _1696_
m user id: 4206
owner id: 21121
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This regulatory part is made up of the Arabinose-Inducible promoter, pBAD, and its transcriptional inhibitor/activator, AraC. AraC inhibits gene expression in the absence of Arabinose by binding to the operator units present within the AraC and pBAD promoter sequences, and producing a DNA loop that prevents the RNA polymerase and CAP from binding and initiating transcription. When Arabinose is supplied, this binds to the AraC dimer and releases it from the operator site found in the pBAD promoter, at the same time it enhances transcription by allowing binding to the enhancer sites upstream.
mutable provenance: This part was cloned out of existing templates (such as BBa_K325218, BBa_325219, all of which contain sfGFP downstream of the pBAD promoter)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: pBAD
sequence annotation: PC
sequence annotation: AraC
sequence: https://synbiohub.org/public/igem/BBa_K1321333_sequence/1

pBAD
type: SequenceAnnotation
persistent identity: annotation2429239
display id: annotation2429239
version: 1
top level: BBa_K1321333
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1321333/annotation2429239/range2429239/1

https://synbiohub.org/public/igem/BBa_K1321333/annotation2429239/range2429239/1
type: Range
persistent identity: range2429239
display id: range2429239
version: 1
direction: direction/forward
top level: BBa_K1321333
start: 1116
end: 1210
orientation: inline

PC
type: SequenceAnnotation
persistent identity: annotation2429238
display id: annotation2429238
version: 1
top level: BBa_K1321333
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1321333/annotation2429238/range2429238/1

https://synbiohub.org/public/igem/BBa_K1321333/annotation2429238/range2429238/1
type: Range
persistent identity: range2429238
display id: range2429238
version: 1
direction: direction/reverse
top level: BBa_K1321333
start: 1042
end: 1115
orientation: inline

AraC
type: SequenceAnnotation
persistent identity: annotation2429237
display id: annotation2429237
version: 1
top level: BBa_K1321333
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1321333/annotation2429237/range2429237/1

https://synbiohub.org/public/igem/BBa_K1321333/annotation2429237/range2429237/1
type: Range
persistent identity: range2429237
display id: range2429237
version: 1
direction: direction/reverse
top level: BBa_K1321333
start: 1
end: 1041
orientation: inline

SspDnaX(N)
type: ComponentDefinition
persistent identity: BBa K1362110
display id: BBa_K1362110
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS + SspDnaX N-intein RFC[105] assembly construct (with His6)
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B??scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch??fer, Carolin Schmelas, Silvan Schmitz, Max Waldha
created: 2014-10-08T11:00:00Z
modified: 2015-05-08T01:10:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1738_
m user id: 0
owner id: 12377
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: ???
mutable notes: ???
mutable provenance: ???
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1362430
component: BBa_K1362421
component: BBa_K1362414
component: BBa_K1362422
component: BBa_K1362415
component: BBa_K1362402
component: BBa_K1362417
component: BBa_G0000
component: BBa_K1362090
component: BBa_K1362437
sequence annotation: BBa_K1362417
sequence annotation: BBa_K1362414
sequence annotation: BBa_K1362421
sequence annotation: BBa_K1362402
sequence annotation: BBa_K1362430
sequence annotation: BBa_K1362437
sequence annotation: BBa_G0000
sequence annotation: BBa_K1362422
sequence annotation: BBa_K1362090
sequence annotation: BBa_K1362415
sequence: https://synbiohub.org/public/igem/BBa_K1362110_sequence/1

BBa_K1362430
type: Component
persistent identity: component2410353
display id: component2410353
version: 1
top level: SspDnaX(N)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362430/1

BBa_K1362421
type: Component
persistent identity: component2410355
display id: component2410355
version: 1
top level: SspDnaX(N)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362421/1

BBa_K1362414
type: Component
persistent identity: component2410324
display id: component2410324
version: 1
top level: SspDnaX(N)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362414/1

BBa_K1362422
type: Component
persistent identity: component2410345
display id: component2410345
version: 1
top level: SspDnaX(N)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362422/1

BBa_K1362415
type: Component
persistent identity: component2410346
display id: component2410346
version: 1
top level: SspDnaX(N)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362415/1

BBa_K1362402
type: Component
persistent identity: component2410347
display id: component2410347
version: 1
top level: SspDnaX(N)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362402/1

BBa_K1362417
type: Component
persistent identity: component2410350
display id: component2410350
version: 1
top level: SspDnaX(N)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362417/1

BBa_G0000
type: Component
persistent identity: component2410323
display id: component2410323
version: 1
top level: SspDnaX(N)
access: public
definition: https://synbiohub.org/public/igem/BBa_G0000/1

BBa_K1362090
type: Component
persistent identity: component2410322
display id: component2410322
version: 1
top level: SspDnaX(N)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362090/1

BBa_K1362437
type: Component
persistent identity: component2410357
display id: component2410357
version: 1
top level: SspDnaX(N)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362437/1

BBa_K1362417
type: SequenceAnnotation
persistent identity: annotation2410350
display id: annotation2410350
version: 1
top level: SspDnaX(N)
location: https://synbiohub.org/public/igem/BBa_K1362110/annotation2410350/range2410350/1
component: BBa_K1362417

https://synbiohub.org/public/igem/BBa_K1362110/annotation2410350/range2410350/1
type: Range
persistent identity: range2410350
display id: range2410350
version: 1
direction: direction/reverse
top level: SspDnaX(N)
start: 1503
end: 1508
orientation: inline

BBa_K1362414
type: SequenceAnnotation
persistent identity: annotation2410324
display id: annotation2410324
version: 1
top level: SspDnaX(N)
location: https://synbiohub.org/public/igem/BBa_K1362110/annotation2410324/range2410324/1
component: BBa_K1362414

https://synbiohub.org/public/igem/BBa_K1362110/annotation2410324/range2410324/1
type: Range
persistent identity: range2410324
display id: range2410324
version: 1
direction: direction/reverse
top level: SspDnaX(N)
start: 36
end: 38
orientation: inline

BBa_K1362421
type: SequenceAnnotation
persistent identity: annotation2410355
display id: annotation2410355
version: 1
top level: SspDnaX(N)
location: https://synbiohub.org/public/igem/BBa_K1362110/annotation2410355/range2410355/1
component: BBa_K1362421

https://synbiohub.org/public/igem/BBa_K1362110/annotation2410355/range2410355/1
type: Range
persistent identity: range2410355
display id: range2410355
version: 1
direction: direction/reverse
top level: SspDnaX(N)
start: 1533
end: 1536
orientation: inline

BBa_K1362402
type: SequenceAnnotation
persistent identity: annotation2410347
display id: annotation2410347
version: 1
top level: SspDnaX(N)
location: https://synbiohub.org/public/igem/BBa_K1362110/annotation2410347/range2410347/1
component: BBa_K1362402

https://synbiohub.org/public/igem/BBa_K1362110/annotation2410347/range2410347/1
type: Range
persistent identity: range2410347
display id: range2410347
version: 1
direction: direction/reverse
top level: SspDnaX(N)
start: 1126
end: 1502
orientation: inline

BBa_K1362430
type: SequenceAnnotation
persistent identity: annotation2410353
display id: annotation2410353
version: 1
top level: SspDnaX(N)
location: https://synbiohub.org/public/igem/BBa_K1362110/annotation2410353/range2410353/1
component: BBa_K1362430

https://synbiohub.org/public/igem/BBa_K1362110/annotation2410353/range2410353/1
type: Range
persistent identity: range2410353
display id: range2410353
version: 1
direction: direction/reverse
top level: SspDnaX(N)
start: 1509
end: 1532
orientation: inline

BBa_K1362437
type: SequenceAnnotation
persistent identity: annotation2410357
display id: annotation2410357
version: 1
top level: SspDnaX(N)
location: https://synbiohub.org/public/igem/BBa_K1362110/annotation2410357/range2410357/1
component: BBa_K1362437

https://synbiohub.org/public/igem/BBa_K1362110/annotation2410357/range2410357/1
type: Range
persistent identity: range2410357
display id: range2410357
version: 1
direction: direction/reverse
top level: SspDnaX(N)
start: 1537
end: 1539
orientation: inline

BBa_G0000
type: SequenceAnnotation
persistent identity: annotation2410323
display id: annotation2410323
version: 1
top level: SspDnaX(N)
location: https://synbiohub.org/public/igem/BBa_K1362110/annotation2410323/range2410323/1
component: BBa_G0000

https://synbiohub.org/public/igem/BBa_K1362110/annotation2410323/range2410323/1
type: Range
persistent identity: range2410323
display id: range2410323
version: 1
direction: direction/reverse
top level: SspDnaX(N)
start: 30
end: 35
orientation: inline

BBa_K1362422
type: SequenceAnnotation
persistent identity: annotation2410345
display id: annotation2410345
version: 1
top level: SspDnaX(N)
location: https://synbiohub.org/public/igem/BBa_K1362110/annotation2410345/range2410345/1
component: BBa_K1362422

https://synbiohub.org/public/igem/BBa_K1362110/annotation2410345/range2410345/1
type: Range
persistent identity: range2410345
display id: range2410345
version: 1
direction: direction/reverse
top level: SspDnaX(N)
start: 39
end: 1121
orientation: inline

BBa_K1362090
type: SequenceAnnotation
persistent identity: annotation2410322
display id: annotation2410322
version: 1
top level: SspDnaX(N)
location: https://synbiohub.org/public/igem/BBa_K1362110/annotation2410322/range2410322/1
component: BBa_K1362090

https://synbiohub.org/public/igem/BBa_K1362110/annotation2410322/range2410322/1
type: Range
persistent identity: range2410322
display id: range2410322
version: 1
direction: direction/reverse
top level: SspDnaX(N)
start: 1
end: 29
orientation: inline

BBa_K1362415
type: SequenceAnnotation
persistent identity: annotation2410346
display id: annotation2410346
version: 1
top level: SspDnaX(N)
location: https://synbiohub.org/public/igem/BBa_K1362110/annotation2410346/range2410346/1
component: BBa_K1362415

https://synbiohub.org/public/igem/BBa_K1362110/annotation2410346/range2410346/1
type: Range
persistent identity: range2410346
display id: range2410346
version: 1
direction: direction/reverse
top level: SspDnaX(N)
start: 1122
end: 1125
orientation: inline

BBa_K1763425
type: ComponentDefinition
persistent identity: BBa K1763425
display id: BBa_K1763425
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: MaSp2-3(T7)
creator: Vinson Lam
created: 2015-09-14T11:00:00Z
modified: 2015-09-15T12:19:04Z
discontinued: false
dominant: false
group u list: _2185_
m user id: 27136
owner id: 27136
owning group id: 9
status: status/Planning
bookmark: false
mutable description: MaSp2 3-mer in a T7-RBS expression vector (BBa_K552998)
mutable notes: no.
mutable provenance: Nephila clavipes
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K525998
component: BBa_K1763424
sequence annotation: BBa_K1763424
sequence annotation: BBa_K525998
sequence: https://synbiohub.org/public/igem/BBa_K1763425_sequence/1

BBa_K525998
type: Component
persistent identity: component2456224
display id: component2456224
version: 1
top level: BBa_K1763425
access: public
definition: https://synbiohub.org/public/igem/BBa_K525998/1

BBa_K1763424
type: Component
persistent identity: component2456225
display id: component2456225
version: 1
top level: BBa_K1763425
access: public
definition: BBa_K1763424

BBa_K1763424
type: SequenceAnnotation
persistent identity: annotation2456225
display id: annotation2456225
version: 1
top level: BBa_K1763425
location: https://synbiohub.org/public/igem/BBa_K1763425/annotation2456225/range2456225/1
component: BBa_K1763424

https://synbiohub.org/public/igem/BBa_K1763425/annotation2456225/range2456225/1
type: Range
persistent identity: range2456225
display id: range2456225
version: 1
direction: direction/reverse
top level: BBa_K1763425
start: 39
end: 395
orientation: inline

BBa_K525998
type: SequenceAnnotation
persistent identity: annotation2456224
display id: annotation2456224
version: 1
top level: BBa_K1763425
location: https://synbiohub.org/public/igem/BBa_K1763425/annotation2456224/range2456224/1
component: BBa_K525998

https://synbiohub.org/public/igem/BBa_K1763425/annotation2456224/range2456224/1
type: Range
persistent identity: range2456224
display id: range2456224
version: 1
direction: direction/reverse
top level: BBa_K1763425
start: 1
end: 32
orientation: inline

BBa_K1362056
type: ComponentDefinition
persistent identity: BBa K1362056
display id: BBa_K1362056
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Myristoilation signal sequence as RFC[105] N-terminal insert
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B??scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch??fer, Carolin Schmelas, Silvan Schmitz, Max Waldha
created: 2014-10-07T11:00:00Z
modified: 2015-05-08T01:10:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1738_
m user id: 0
owner id: 22830
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1362423
component: BBa_K1362425
component: BBa_I712026
component: BBa_K1362444
component: BBa_K1362415
sequence annotation: BBa_K1362423
sequence annotation: BBa_I712026
sequence annotation: BBa_K1362415
sequence annotation: BBa_K1362444
sequence annotation: BBa_K1362425
sequence: https://synbiohub.org/public/igem/BBa_K1362056_sequence/1

BBa_K1362423
type: Component
persistent identity: component2409722
display id: component2409722
version: 1
top level: BBa_K1362056
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362423/1

BBa_K1362425
type: Component
persistent identity: component2409718
display id: component2409718
version: 1
top level: BBa_K1362056
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362425/1

BBa_I712026
type: Component
persistent identity: component2409719
display id: component2409719
version: 1
top level: BBa_K1362056
access: public
definition: https://synbiohub.org/public/igem/BBa_I712026/1

BBa_K1362444
type: Component
persistent identity: component2409720
display id: component2409720
version: 1
top level: BBa_K1362056
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362444/1

BBa_K1362415
type: Component
persistent identity: component2409721
display id: component2409721
version: 1
top level: BBa_K1362056
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362415/1

BBa_K1362423
type: SequenceAnnotation
persistent identity: annotation2409722
display id: annotation2409722
version: 1
top level: BBa_K1362056
location: https://synbiohub.org/public/igem/BBa_K1362056/annotation2409722/range2409722/1
component: BBa_K1362423

https://synbiohub.org/public/igem/BBa_K1362056/annotation2409722/range2409722/1
type: Range
persistent identity: range2409722
display id: range2409722
version: 1
direction: direction/reverse
top level: BBa_K1362056
start: 51
end: 57
orientation: inline

BBa_I712026
type: SequenceAnnotation
persistent identity: annotation2409719
display id: annotation2409719
version: 1
top level: BBa_K1362056
location: https://synbiohub.org/public/igem/BBa_K1362056/annotation2409719/range2409719/1
component: BBa_I712026

https://synbiohub.org/public/igem/BBa_K1362056/annotation2409719/range2409719/1
type: Range
persistent identity: range2409719
display id: range2409719
version: 1
direction: direction/reverse
top level: BBa_K1362056
start: 8
end: 28
orientation: inline

BBa_K1362415
type: SequenceAnnotation
persistent identity: annotation2409721
display id: annotation2409721
version: 1
top level: BBa_K1362056
location: https://synbiohub.org/public/igem/BBa_K1362056/annotation2409721/range2409721/1
component: BBa_K1362415

https://synbiohub.org/public/igem/BBa_K1362056/annotation2409721/range2409721/1
type: Range
persistent identity: range2409721
display id: range2409721
version: 1
direction: direction/reverse
top level: BBa_K1362056
start: 47
end: 50
orientation: inline

BBa_K1362444
type: SequenceAnnotation
persistent identity: annotation2409720
display id: annotation2409720
version: 1
top level: BBa_K1362056
location: https://synbiohub.org/public/igem/BBa_K1362056/annotation2409720/range2409720/1
component: BBa_K1362444

https://synbiohub.org/public/igem/BBa_K1362056/annotation2409720/range2409720/1
type: Range
persistent identity: range2409720
display id: range2409720
version: 1
direction: direction/reverse
top level: BBa_K1362056
start: 29
end: 46
orientation: inline

BBa_K1362425
type: SequenceAnnotation
persistent identity: annotation2409718
display id: annotation2409718
version: 1
top level: BBa_K1362056
location: https://synbiohub.org/public/igem/BBa_K1362056/annotation2409718/range2409718/1
component: BBa_K1362425

https://synbiohub.org/public/igem/BBa_K1362056/annotation2409718/range2409718/1
type: Range
persistent identity: range2409718
display id: range2409718
version: 1
direction: direction/reverse
top level: BBa_K1362056
start: 1
end: 6
orientation: inline

BBa_K1497026
type: ComponentDefinition
persistent identity: BBa K1497026
display id: BBa_K1497026
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PDZ-Domain
creator: Sascha Hein, Rene Sahm
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:45Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1877_
m user id: 0
owner id: 12466
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: d
mutable notes: d
mutable provenance: d
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1497026_sequence/1

DspB
type: ComponentDefinition
persistent identity: BBa K1659200
display id: BBa_K1659200
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Dispersin B, an antibiofilm enzyme that specifically hydrolyses PGA polymers
creator: Wei Chung Kong
created: 2015-08-27T11:00:00Z
modified: 2015-09-12T03:59:05Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2077_
m user id: 24524
owner id: 24524
owning group id: 9
status: status/Planning
bookmark: false
mutable description: to be updated
mutable notes: to be updated
mutable provenance: to be updated
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: stop
sequence annotation: start
sequence annotation: Hisx6
sequence annotation: DspB
sequence: https://synbiohub.org/public/igem/BBa_K1659200_sequence/1

stop
type: SequenceAnnotation
persistent identity: annotation2449112
display id: annotation2449112
version: 1
top level: DspB
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1659200/annotation2449112/range2449112/1

https://synbiohub.org/public/igem/BBa_K1659200/annotation2449112/range2449112/1
type: Range
persistent identity: range2449112
display id: range2449112
version: 1
direction: direction/forward
top level: DspB
start: 1105
end: 1110
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2449109
display id: annotation2449109
version: 1
top level: DspB
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1659200/annotation2449109/range2449109/1

https://synbiohub.org/public/igem/BBa_K1659200/annotation2449109/range2449109/1
type: Range
persistent identity: range2449109
display id: range2449109
version: 1
direction: direction/forward
top level: DspB
start: 1
end: 3
orientation: inline

Hisx6
type: SequenceAnnotation
persistent identity: annotation2449111
display id: annotation2449111
version: 1
top level: DspB
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1659200/annotation2449111/range2449111/1

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DspB
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BBa_K1321154
type: ComponentDefinition
persistent identity: BBa K1321154
display id: BBa_K1321154
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CBDclos fused to metallothionein Fmt
creator: Gabriella Santosa
created: 2014-10-08T11:00:00Z
modified: 2015-06-17T12:23:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 4206
owner id: 20961
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: GATC
mutable notes: GATC
mutable provenance: GATC
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_B0105
component: BBa_K1321012
component: BBa_K1321002
sequence annotation: BBa_B0105
sequence annotation: BBa_K1321002
sequence annotation: BBa_K1321012
sequence: https://synbiohub.org/public/igem/BBa_K1321154_sequence/1

BBa_B0105
type: Component
persistent identity: component2415055
display id: component2415055
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

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display id: component2415057
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_K1321012/1

BBa_K1321002
type: Component
persistent identity: component2415053
display id: component2415053
version: 1
top level: BBa_K1321154
access: public
definition: https://synbiohub.org/public/igem/BBa_K1321002/1

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2415055
display id: annotation2415055
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component: BBa_B0105

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BBa_K1321002
type: SequenceAnnotation
persistent identity: annotation2415053
display id: annotation2415053
version: 1
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location: https://synbiohub.org/public/igem/BBa_K1321154/annotation2415053/range2415053/1
component: BBa_K1321002

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display id: range2415053
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type: SequenceAnnotation
persistent identity: annotation2415057
display id: annotation2415057
version: 1
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location: https://synbiohub.org/public/igem/BBa_K1321154/annotation2415057/range2415057/1
component: BBa_K1321012

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type: Range
persistent identity: range2415057
display id: range2415057
version: 1
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start: 307
end: 504
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BBa_K1349008
type: ComponentDefinition
persistent identity: BBa K1349008
display id: BBa_K1349008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: sh3pep-lza
creator: Alexia Satouf, Clara Bouyx, Aimeric Agaoua, Lambert Antoni, Vincent Castel
created: 2014-10-05T11:00:00Z
modified: 2015-05-08T01:10:01Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1724_
m user id: 0
owner id: 6328
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: SH3pep-LZA is a link wich mediates proteins interactions.
mutable notes: xxx
mutable provenance: Amplified from pWW2181,Whitaker et al. PNAS 2012
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1349008_sequence/1

BBa_K1486022
type: ComponentDefinition
persistent identity: BBa K1486022
display id: BBa_K1486022
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Renilla Luciferase
creator: EPFL iGem team 2014
created: 2014-09-14T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part is the full renilla luciferase. The split protein has also been submitted as biobricks: BBa_K1486021.
mutable notes: to edit
mutable provenance: To edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K1486020
component: BBa_B0034
component: BBa_I0500
component: BBa_K1486019
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sequence annotation: BBa_B0034
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sequence: https://synbiohub.org/public/igem/BBa_K1486022_sequence/1

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type: Component
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display id: component2383475
version: 1
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access: public
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BBa_B0034
type: Component
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display id: component2383473
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access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_I0500
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display id: component2383471
version: 1
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access: public
definition: pBad/araC

BBa_K1486019
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version: 1
top level: BBa_K1486022
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486019/1

BBa_I0500
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version: 1
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BBa_K1486020
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display id: range2383475
version: 1
direction: direction/reverse
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start: 1553
end: 2158
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BBa_K1632011
type: ComponentDefinition
persistent identity: BBa K1632011
display id: BBa_K1632011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: fimE (wild-type)
creator: Riku Shinohara
created: 2015-08-17T11:00:00Z
modified: 2015-09-15T01:08:25Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2049_
m user id: 24760
owner id: 22687
owning group id: 9
status: status/Planning
bookmark: false
mutable description: a
mutable notes: a
mutable provenance: a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1632011_sequence/1

BBa_K1362111
type: ComponentDefinition
persistent identity: BBa K1362111
display id: BBa_K1362111
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS + SspDnaX C-intein RFC[105] assembly construct (with His6)
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B??scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch??fer, Carolin Schmelas, Silvan Schmitz, Max Waldha
created: 2014-10-08T11:00:00Z
modified: 2015-05-08T01:10:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1738_
m user id: 0
owner id: 12377
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: ???
mutable notes: ???
mutable provenance: ???
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1362419
component: BBa_K1362422
component: BBa_K1362418
component: BBa_K1362465
component: BBa_K1362467
component: BBa_K1362090
component: BBa_K1362414
component: BBa_G0000
component: BBa_K1362403
component: BBa_K1362431
component: BBa_K1362421
sequence annotation: BBa_K1362422
sequence annotation: BBa_K1362418
sequence annotation: BBa_K1362421
sequence annotation: BBa_G0000
sequence annotation: BBa_K1362465
sequence annotation: BBa_K1362431
sequence annotation: BBa_K1362419
sequence annotation: BBa_K1362467
sequence annotation: BBa_K1362403
sequence annotation: BBa_K1362090
sequence annotation: BBa_K1362414
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version: 1
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access: public
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BBa_K1362422
type: Component
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display id: component2410391
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_K1362422/1

BBa_K1362418
type: Component
persistent identity: component2410367
display id: component2410367
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_K1362418/1

BBa_K1362465
type: Component
persistent identity: component2410394
display id: component2410394
version: 1
top level: BBa_K1362111
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362465/1

BBa_K1362467
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persistent identity: component2410368
display id: component2410368
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_K1362467/1

BBa_K1362090
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persistent identity: component2410361
display id: component2410361
version: 1
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definition: https://synbiohub.org/public/igem/BBa_K1362090/1

BBa_K1362414
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persistent identity: component2410363
display id: component2410363
version: 1
top level: BBa_K1362111
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362414/1

BBa_G0000
type: Component
persistent identity: component2410362
display id: component2410362
version: 1
top level: BBa_K1362111
access: public
definition: https://synbiohub.org/public/igem/BBa_G0000/1

BBa_K1362403
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persistent identity: component2410369
display id: component2410369
version: 1
top level: BBa_K1362111
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362403/1

BBa_K1362431
type: Component
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display id: component2410366
version: 1
top level: BBa_K1362111
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362431/1

BBa_K1362421
type: Component
persistent identity: component2410393
display id: component2410393
version: 1
top level: BBa_K1362111
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362421/1

BBa_K1362422
type: SequenceAnnotation
persistent identity: annotation2410391
display id: annotation2410391
version: 1
top level: BBa_K1362111
location: https://synbiohub.org/public/igem/BBa_K1362111/annotation2410391/range2410391/1
component: BBa_K1362422

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type: Range
persistent identity: range2410391
display id: range2410391
version: 1
direction: direction/reverse
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start: 84
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orientation: inline

BBa_K1362418
type: SequenceAnnotation
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location: https://synbiohub.org/public/igem/BBa_K1362111/annotation2410367/range2410367/1
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version: 1
direction: direction/reverse
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start: 60
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orientation: inline

BBa_K1362421
type: SequenceAnnotation
persistent identity: annotation2410393
display id: annotation2410393
version: 1
top level: BBa_K1362111
location: https://synbiohub.org/public/igem/BBa_K1362111/annotation2410393/range2410393/1
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persistent identity: range2410393
display id: range2410393
version: 1
direction: direction/reverse
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start: 1167
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orientation: inline

BBa_G0000
type: SequenceAnnotation
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top level: BBa_K1362111
location: https://synbiohub.org/public/igem/BBa_K1362111/annotation2410362/range2410362/1
component: BBa_G0000

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type: Range
persistent identity: range2410362
display id: range2410362
version: 1
direction: direction/reverse
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start: 30
end: 35
orientation: inline

BBa_K1362465
type: SequenceAnnotation
persistent identity: annotation2410394
display id: annotation2410394
version: 1
top level: BBa_K1362111
location: https://synbiohub.org/public/igem/BBa_K1362111/annotation2410394/range2410394/1
component: BBa_K1362465

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type: Range
persistent identity: range2410394
display id: range2410394
version: 1
direction: direction/reverse
top level: BBa_K1362111
start: 1171
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orientation: inline

BBa_K1362431
type: SequenceAnnotation
persistent identity: annotation2410366
display id: annotation2410366
version: 1
top level: BBa_K1362111
location: https://synbiohub.org/public/igem/BBa_K1362111/annotation2410366/range2410366/1
component: BBa_K1362431

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type: Range
persistent identity: range2410366
display id: range2410366
version: 1
direction: direction/reverse
top level: BBa_K1362111
start: 39
end: 59
orientation: inline

BBa_K1362419
type: SequenceAnnotation
persistent identity: annotation2410370
display id: annotation2410370
version: 1
top level: BBa_K1362111
location: https://synbiohub.org/public/igem/BBa_K1362111/annotation2410370/range2410370/1
component: BBa_K1362419

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type: Range
persistent identity: range2410370
display id: range2410370
version: 1
direction: direction/reverse
top level: BBa_K1362111
start: 80
end: 83
orientation: inline

BBa_K1362467
type: SequenceAnnotation
persistent identity: annotation2410368
display id: annotation2410368
version: 1
top level: BBa_K1362111
location: https://synbiohub.org/public/igem/BBa_K1362111/annotation2410368/range2410368/1
component: BBa_K1362467

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type: Range
persistent identity: range2410368
display id: range2410368
version: 1
direction: direction/reverse
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start: 64
end: 65
orientation: inline

BBa_K1362403
type: SequenceAnnotation
persistent identity: annotation2410369
display id: annotation2410369
version: 1
top level: BBa_K1362111
location: https://synbiohub.org/public/igem/BBa_K1362111/annotation2410369/range2410369/1
component: BBa_K1362403

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type: Range
persistent identity: range2410369
display id: range2410369
version: 1
direction: direction/reverse
top level: BBa_K1362111
start: 66
end: 79
orientation: inline

BBa_K1362090
type: SequenceAnnotation
persistent identity: annotation2410361
display id: annotation2410361
version: 1
top level: BBa_K1362111
location: https://synbiohub.org/public/igem/BBa_K1362111/annotation2410361/range2410361/1
component: BBa_K1362090

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type: Range
persistent identity: range2410361
display id: range2410361
version: 1
direction: direction/reverse
top level: BBa_K1362111
start: 1
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BBa_K1362414
type: SequenceAnnotation
persistent identity: annotation2410363
display id: annotation2410363
version: 1
top level: BBa_K1362111
location: https://synbiohub.org/public/igem/BBa_K1362111/annotation2410363/range2410363/1
component: BBa_K1362414

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type: Range
persistent identity: range2410363
display id: range2410363
version: 1
direction: direction/reverse
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start: 36
end: 38
orientation: inline

BBa_K1497010
type: ComponentDefinition
persistent identity: BBa K1497010
display id: BBa_K1497010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Dihydroflavonol 4-reductase (DFR)
creator: Bastian Wagner, Christian Mende, Andreas Schmidt, Max Dombrowsky
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1877_
m user id: 0
owner id: 12466
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: d
mutable notes: d
mutable provenance: d
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1497010_sequence/1

BBa_K1491016
type: ComponentDefinition
persistent identity: BBa K1491016
display id: BBa_K1491016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lac Inducible Codon Optimized KillerRed
creator: Danielle Peters
created: 2014-08-03T11:00:00Z
modified: 2015-05-08T01:10:44Z
discontinued: false
dominant: false
group u list: _1871_
m user id: 0
owner id: 22521
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Codon optimized KillerRed with WTLac promoter allows for induction of KillerRed expression with IPTG.
mutable notes: Lac inducible codon optimized KillerRed
mutable provenance: The original KillerRed is BBa_K1184000.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1491015
component: BBa_J04500
sequence annotation: BBa_J04500
sequence annotation: BBa_K1491015
sequence: https://synbiohub.org/public/igem/BBa_K1491016_sequence/1

BBa_K1491015
type: Component
persistent identity: component2380355
display id: component2380355
version: 1
top level: BBa_K1491016
access: public
definition: BBa_K1491015

BBa_J04500
type: Component
persistent identity: component2380353
display id: component2380353
version: 1
top level: BBa_K1491016
access: public
definition: https://synbiohub.org/public/igem/BBa_J04500/1

BBa_J04500
type: SequenceAnnotation
persistent identity: annotation2380353
display id: annotation2380353
version: 1
top level: BBa_K1491016
location: https://synbiohub.org/public/igem/BBa_K1491016/annotation2380353/range2380353/1
component: BBa_J04500

https://synbiohub.org/public/igem/BBa_K1491016/annotation2380353/range2380353/1
type: Range
persistent identity: range2380353
display id: range2380353
version: 1
direction: direction/reverse
top level: BBa_K1491016
start: 1
end: 220
orientation: inline

BBa_K1491015
type: SequenceAnnotation
persistent identity: annotation2380355
display id: annotation2380355
version: 1
top level: BBa_K1491016
location: https://synbiohub.org/public/igem/BBa_K1491016/annotation2380355/range2380355/1
component: BBa_K1491015

https://synbiohub.org/public/igem/BBa_K1491016/annotation2380355/range2380355/1
type: Range
persistent identity: range2380355
display id: range2380355
version: 1
direction: direction/reverse
top level: BBa_K1491016
start: 227
end: 967
orientation: inline

pFig2c
type: ComponentDefinition
persistent identity: BBa K1829002
display id: BBa_K1829002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pFig2c: Mating factor alpha responsive promoter
creator: Nicholas Suen
created: 2015-09-08T11:00:00Z
modified: 2015-09-14T11:40:07Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2255_
m user id: 18557
owner id: 27842
owning group id: 9
status: status/Planning
bookmark: false
mutable description: pFig2c is a mating factor alpha responsive promoter, meaning that it is activated by the presence of mating factor alpha. It is endogenous in S. Cerevisiae cells and is followed by the Fig2 gene. The Fig2 gene is a part of the mating pathway in S. Cerevisiae, it contributes to maintaining cell wall integrity when the cells project ("shmoo") towards each other.
mutable notes: We changed nucleeotide 22 from adenine to thymine in order to eliminate the illegal Pst1 cut site.
mutable provenance: pFig2c is found in the genomic sequence of MATa S. cerevisiae.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K1829002_sequence/1

BBa_K1806005
type: ComponentDefinition
persistent identity: BBa K1806005
display id: BBa_K1806005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7+ ibPB RNA Thermometer + RFP
creator: İBRAHİM YASİR ORHAN
created: 2015-09-16T11:00:00Z
modified: 2015-09-21T11:27:36Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _973_2231_
m user id: 11156
owner id: 11153
owning group id: 9
status: status/Planning
bookmark: true
mutable description: The part is designed to produce RFP in higher temperatures. The RNA Thermometer acts as a heat-shock RBS.
mutable notes: none
mutable provenance: The part is composed of distinct parts from various origins.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence: https://synbiohub.org/public/igem/BBa_K1806005_sequence/1

SspGyrB(N)
type: ComponentDefinition
persistent identity: BBa K1362120
display id: BBa_K1362120
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS + SspGyrB N-intein RFC[105] assembly construct (with His6)
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B??scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch??fer, Carolin Schmelas, Silvan Schmitz, Max Waldha
created: 2014-10-08T11:00:00Z
modified: 2015-05-08T01:10:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1738_
m user id: 0
owner id: 12377
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: ???
mutable notes: ???
mutable provenance: ???
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1362414
component: BBa_K1362406
component: BBa_K1362437
component: BBa_K1362417
component: BBa_G0000
component: BBa_K1362090
component: BBa_K1362415
component: BBa_K1362430
component: BBa_K1362422
component: BBa_K1362421
sequence annotation: BBa_K1362417
sequence annotation: BBa_K1362430
sequence annotation: BBa_K1362406
sequence annotation: BBa_K1362437
sequence annotation: BBa_K1362421
sequence annotation: BBa_K1362415
sequence annotation: BBa_K1362422
sequence annotation: BBa_K1362414
sequence annotation: BBa_K1362090
sequence annotation: BBa_G0000
sequence: https://synbiohub.org/public/igem/BBa_K1362120_sequence/1

BBa_K1362414
type: Component
persistent identity: component2410399
display id: component2410399
version: 1
top level: SspGyrB(N)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362414/1

BBa_K1362406
type: Component
persistent identity: component2410422
display id: component2410422
version: 1
top level: SspGyrB(N)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362406/1

BBa_K1362437
type: Component
persistent identity: component2410432
display id: component2410432
version: 1
top level: SspGyrB(N)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362437/1

BBa_K1362417
type: Component
persistent identity: component2410425
display id: component2410425
version: 1
top level: SspGyrB(N)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362417/1

BBa_G0000
type: Component
persistent identity: component2410398
display id: component2410398
version: 1
top level: SspGyrB(N)
access: public
definition: https://synbiohub.org/public/igem/BBa_G0000/1

BBa_K1362090
type: Component
persistent identity: component2410397
display id: component2410397
version: 1
top level: SspGyrB(N)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362090/1

BBa_K1362415
type: Component
persistent identity: component2410421
display id: component2410421
version: 1
top level: SspGyrB(N)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362415/1

BBa_K1362430
type: Component
persistent identity: component2410428
display id: component2410428
version: 1
top level: SspGyrB(N)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362430/1

BBa_K1362422
type: Component
persistent identity: component2410420
display id: component2410420
version: 1
top level: SspGyrB(N)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362422/1

BBa_K1362421
type: Component
persistent identity: component2410430
display id: component2410430
version: 1
top level: SspGyrB(N)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362421/1

BBa_K1362417
type: SequenceAnnotation
persistent identity: annotation2410425
display id: annotation2410425
version: 1
top level: SspGyrB(N)
location: https://synbiohub.org/public/igem/BBa_K1362120/annotation2410425/range2410425/1
component: BBa_K1362417

https://synbiohub.org/public/igem/BBa_K1362120/annotation2410425/range2410425/1
type: Range
persistent identity: range2410425
display id: range2410425
version: 1
direction: direction/reverse
top level: SspGyrB(N)
start: 1572
end: 1577
orientation: inline

BBa_K1362430
type: SequenceAnnotation
persistent identity: annotation2410428
display id: annotation2410428
version: 1
top level: SspGyrB(N)
location: https://synbiohub.org/public/igem/BBa_K1362120/annotation2410428/range2410428/1
component: BBa_K1362430

https://synbiohub.org/public/igem/BBa_K1362120/annotation2410428/range2410428/1
type: Range
persistent identity: range2410428
display id: range2410428
version: 1
direction: direction/reverse
top level: SspGyrB(N)
start: 1578
end: 1601
orientation: inline

BBa_K1362406
type: SequenceAnnotation
persistent identity: annotation2410422
display id: annotation2410422
version: 1
top level: SspGyrB(N)
location: https://synbiohub.org/public/igem/BBa_K1362120/annotation2410422/range2410422/1
component: BBa_K1362406

https://synbiohub.org/public/igem/BBa_K1362120/annotation2410422/range2410422/1
type: Range
persistent identity: range2410422
display id: range2410422
version: 1
direction: direction/reverse
top level: SspGyrB(N)
start: 1126
end: 1571
orientation: inline

BBa_K1362437
type: SequenceAnnotation
persistent identity: annotation2410432
display id: annotation2410432
version: 1
top level: SspGyrB(N)
location: https://synbiohub.org/public/igem/BBa_K1362120/annotation2410432/range2410432/1
component: BBa_K1362437

https://synbiohub.org/public/igem/BBa_K1362120/annotation2410432/range2410432/1
type: Range
persistent identity: range2410432
display id: range2410432
version: 1
direction: direction/reverse
top level: SspGyrB(N)
start: 1606
end: 1608
orientation: inline

BBa_K1362421
type: SequenceAnnotation
persistent identity: annotation2410430
display id: annotation2410430
version: 1
top level: SspGyrB(N)
location: https://synbiohub.org/public/igem/BBa_K1362120/annotation2410430/range2410430/1
component: BBa_K1362421

https://synbiohub.org/public/igem/BBa_K1362120/annotation2410430/range2410430/1
type: Range
persistent identity: range2410430
display id: range2410430
version: 1
direction: direction/reverse
top level: SspGyrB(N)
start: 1602
end: 1605
orientation: inline

BBa_K1362415
type: SequenceAnnotation
persistent identity: annotation2410421
display id: annotation2410421
version: 1
top level: SspGyrB(N)
location: https://synbiohub.org/public/igem/BBa_K1362120/annotation2410421/range2410421/1
component: BBa_K1362415

https://synbiohub.org/public/igem/BBa_K1362120/annotation2410421/range2410421/1
type: Range
persistent identity: range2410421
display id: range2410421
version: 1
direction: direction/reverse
top level: SspGyrB(N)
start: 1122
end: 1125
orientation: inline

BBa_K1362422
type: SequenceAnnotation
persistent identity: annotation2410420
display id: annotation2410420
version: 1
top level: SspGyrB(N)
location: https://synbiohub.org/public/igem/BBa_K1362120/annotation2410420/range2410420/1
component: BBa_K1362422

https://synbiohub.org/public/igem/BBa_K1362120/annotation2410420/range2410420/1
type: Range
persistent identity: range2410420
display id: range2410420
version: 1
direction: direction/reverse
top level: SspGyrB(N)
start: 39
end: 1121
orientation: inline

BBa_K1362414
type: SequenceAnnotation
persistent identity: annotation2410399
display id: annotation2410399
version: 1
top level: SspGyrB(N)
location: https://synbiohub.org/public/igem/BBa_K1362120/annotation2410399/range2410399/1
component: BBa_K1362414

https://synbiohub.org/public/igem/BBa_K1362120/annotation2410399/range2410399/1
type: Range
persistent identity: range2410399
display id: range2410399
version: 1
direction: direction/reverse
top level: SspGyrB(N)
start: 36
end: 38
orientation: inline

BBa_K1362090
type: SequenceAnnotation
persistent identity: annotation2410397
display id: annotation2410397
version: 1
top level: SspGyrB(N)
location: https://synbiohub.org/public/igem/BBa_K1362120/annotation2410397/range2410397/1
component: BBa_K1362090

https://synbiohub.org/public/igem/BBa_K1362120/annotation2410397/range2410397/1
type: Range
persistent identity: range2410397
display id: range2410397
version: 1
direction: direction/reverse
top level: SspGyrB(N)
start: 1
end: 29
orientation: inline

BBa_G0000
type: SequenceAnnotation
persistent identity: annotation2410398
display id: annotation2410398
version: 1
top level: SspGyrB(N)
location: https://synbiohub.org/public/igem/BBa_K1362120/annotation2410398/range2410398/1
component: BBa_G0000

https://synbiohub.org/public/igem/BBa_K1362120/annotation2410398/range2410398/1
type: Range
persistent identity: range2410398
display id: range2410398
version: 1
direction: direction/reverse
top level: SspGyrB(N)
start: 30
end: 35
orientation: inline

BBa_K1763004
type: ComponentDefinition
persistent identity: BBa K1763004
display id: BBa_K1763004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Major Spidroin Protein 2 (MaSp2) with sticky ends CA
creator: Vinson Lam
created: 2015-06-01T11:00:00Z
modified: 2015-09-18T10:53:36Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2185_
m user id: 27136
owner id: 27136
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This part contains the core sequence of MaSp2 that has been assembled with a 5'-CGTG-3' overhang on the 5' end of the sequence and 3'-TCAA-5' overhang on the 3' end of the sequence. This part was designed for use with ICA, along with parts BBa_K1763003 and BBa_K1763004 as an efficient method to assemble multiple MaSp2 sequences together. This setup is to show that this particular set of sticky ends is suitable for ICA. Before using in ICA, this part should be digested with BsaI, a type IIs restriction enzyme, which cuts DNA outside of its recognition site.
mutable notes: The gene sequence for MaSp2 is particularly GC-rich, which may complicate PCR.
mutable provenance: This part is derived from the MaSp2 gene found in the spider N. Clavipes.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: BsaI recognition site (reverse)
sequence annotation: MaSp2 Core
sequence annotation: BsaI recognition site (forward)
sequence annotation: C sticky end
sequence annotation: A sticky end
sequence: https://synbiohub.org/public/igem/BBa_K1763004_sequence/1

BsaI recognition site (reverse)
type: SequenceAnnotation
persistent identity: annotation2432570
display id: annotation2432570
version: 1
top level: BBa_K1763004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1763004/annotation2432570/range2432570/1

https://synbiohub.org/public/igem/BBa_K1763004/annotation2432570/range2432570/1
type: Range
persistent identity: range2432570
display id: range2432570
version: 1
direction: direction/reverse
top level: BBa_K1763004
start: 114
end: 119
orientation: inline

MaSp2 Core
type: SequenceAnnotation
persistent identity: annotation2473129
display id: annotation2473129
version: 1
top level: BBa_K1763004
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763004/annotation2473129/range2473129/1

https://synbiohub.org/public/igem/BBa_K1763004/annotation2473129/range2473129/1
type: Range
persistent identity: range2473129
display id: range2473129
version: 1
direction: direction/forward
top level: BBa_K1763004
start: 10
end: 108
orientation: inline

BsaI recognition site (forward)
type: SequenceAnnotation
persistent identity: annotation2432569
display id: annotation2432569
version: 1
top level: BBa_K1763004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1763004/annotation2432569/range2432569/1

https://synbiohub.org/public/igem/BBa_K1763004/annotation2432569/range2432569/1
type: Range
persistent identity: range2432569
display id: range2432569
version: 1
direction: direction/forward
top level: BBa_K1763004
start: 1
end: 5
orientation: inline

C sticky end
type: SequenceAnnotation
persistent identity: annotation2463764
display id: annotation2463764
version: 1
top level: BBa_K1763004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1763004/annotation2463764/range2463764/1

https://synbiohub.org/public/igem/BBa_K1763004/annotation2463764/range2463764/1
type: Range
persistent identity: range2463764
display id: range2463764
version: 1
direction: direction/forward
top level: BBa_K1763004
start: 7
end: 10
orientation: inline

A sticky end
type: SequenceAnnotation
persistent identity: annotation2463765
display id: annotation2463765
version: 1
top level: BBa_K1763004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1763004/annotation2463765/range2463765/1

https://synbiohub.org/public/igem/BBa_K1763004/annotation2463765/range2463765/1
type: Range
persistent identity: range2463765
display id: range2463765
version: 1
direction: direction/forward
top level: BBa_K1763004
start: 109
end: 112
orientation: inline

BBa_K1758311
type: ComponentDefinition
persistent identity: BBa K1758311
display id: BBa_K1758311
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: mRFP under control of the chromium responsive promoter
creator: Team Bielefeld-CeBiTec 2015
created: 2015-08-29T11:00:00Z
modified: 2015-09-20T08:45:00Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2180_
m user id: 26931
owner id: 26630
owning group id: 9
status: status/Planning
bookmark: false
mutable description: chromium responsive Promoter with RFP for detecition
mutable notes: ...
mutable provenance: chromium VI responsive promoter of ????Ochrobactrum tritici???? 5bvl1
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: ChrP
sequence annotation: mRFP
sequence: https://synbiohub.org/public/igem/BBa_K1758311_sequence/1

ChrP
type: SequenceAnnotation
persistent identity: annotation2440065
display id: annotation2440065
version: 1
top level: BBa_K1758311
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1758311/annotation2440065/range2440065/1

https://synbiohub.org/public/igem/BBa_K1758311/annotation2440065/range2440065/1
type: Range
persistent identity: range2440065
display id: range2440065
version: 1
direction: direction/forward
top level: BBa_K1758311
start: 1
end: 165
orientation: inline

mRFP
type: SequenceAnnotation
persistent identity: annotation2440064
display id: annotation2440064
version: 1
top level: BBa_K1758311
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1758311/annotation2440064/range2440064/1

https://synbiohub.org/public/igem/BBa_K1758311/annotation2440064/range2440064/1
type: Range
persistent identity: range2440064
display id: range2440064
version: 1
direction: direction/forward
top level: BBa_K1758311
start: 171
end: 848
orientation: inline

BBa_K1758203
type: ComponentDefinition
persistent identity: BBa K1758203
display id: BBa_K1758203
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: His-tagged arsenic repressor fused to superfolder GFP under the control of T7 promoter
creator: Team Bielefeld-CeBiTec 2015
created: 2015-08-28T11:00:00Z
modified: 2015-09-18T05:53:38Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2180_
m user id: 26913
owner id: 26913
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Uses T7 promotor and a strong rbs (BBa_K525998), the arsenic repressor linked with superfolding GFP (sfGFP) with a His-Tag. By linking sfGFP we want to detect bound protein via fluorescence.
mutable notes: We clone arsR in T7-RBS-sfGFP and added a His-Tag via PCR and Gibson Assembly.
mutable provenance: This is a composite part which enables a high protein expression and an easy purification of arsR fused to superfolding GFP (sfGFP). The green fluorescence of the culture is a sign for protein expression.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: RBS
sequence annotation: BBa_B0034
sequence annotation: T7 Promoter
sequence annotation: His-Tag
sequence annotation: BBa_B0010
sequence annotation: BBa_K525998
sequence annotation: arsR
sequence annotation: sfGFP
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K1758203_sequence/1

RBS
type: SequenceAnnotation
persistent identity: annotation2440038
display id: annotation2440038
version: 1
top level: BBa_K1758203
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1758203/annotation2440038/range2440038/1

https://synbiohub.org/public/igem/BBa_K1758203/annotation2440038/range2440038/1
type: Range
persistent identity: range2440038
display id: range2440038
version: 1
direction: direction/forward
top level: BBa_K1758203
start: 24
end: 27
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2440036
display id: annotation2440036
version: 1
top level: BBa_K1758203
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1758203/annotation2440036/range2440036/1

https://synbiohub.org/public/igem/BBa_K1758203/annotation2440036/range2440036/1
type: Range
persistent identity: range2440036
display id: range2440036
version: 1
direction: direction/forward
top level: BBa_K1758203
start: 21
end: 32
orientation: inline

T7 Promoter
type: SequenceAnnotation
persistent identity: annotation2440039
display id: annotation2440039
version: 1
top level: BBa_K1758203
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1758203/annotation2440039/range2440039/1

https://synbiohub.org/public/igem/BBa_K1758203/annotation2440039/range2440039/1
type: Range
persistent identity: range2440039
display id: range2440039
version: 1
direction: direction/forward
top level: BBa_K1758203
start: 1
end: 23
orientation: inline

His-Tag
type: SequenceAnnotation
persistent identity: annotation2440040
display id: annotation2440040
version: 1
top level: BBa_K1758203
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1758203/annotation2440040/range2440040/1

https://synbiohub.org/public/igem/BBa_K1758203/annotation2440040/range2440040/1
type: Range
persistent identity: range2440040
display id: range2440040
version: 1
direction: direction/forward
top level: BBa_K1758203
start: 1123
end: 1146
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2440043
display id: annotation2440043
version: 1
top level: BBa_K1758203
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1758203/annotation2440043/range2440043/1

https://synbiohub.org/public/igem/BBa_K1758203/annotation2440043/range2440043/1
type: Range
persistent identity: range2440043
display id: range2440043
version: 1
direction: direction/forward
top level: BBa_K1758203
start: 1155
end: 1234
orientation: inline

BBa_K525998
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role: SO:0000804
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start: 1
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arsR
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sfGFP
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role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1758203/annotation2440042/range2440042/1

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BBa_B0012
type: SequenceAnnotation
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top level: BBa_K1758203
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1758203/annotation2440044/range2440044/1

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start: 1243
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BBa_K1497013
type: ComponentDefinition
persistent identity: BBa K1497013
display id: BBa_K1497013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0034-DFR
creator: Bastian Wagner, Christian Mende, Andreas Schmidt, Max Dombrowsky
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1877_
m user id: 0
owner id: 12466
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: a
mutable notes: a
mutable provenance: a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_B0034
component: BBa_K1497010
sequence annotation: BBa_K1497010
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K1497013_sequence/1

BBa_B0034
type: Component
persistent identity: component2398836
display id: component2398836
version: 1
top level: BBa_K1497013
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1497010
type: Component
persistent identity: component2398837
display id: component2398837
version: 1
top level: BBa_K1497013
access: public
definition: BBa_K1497010

BBa_K1497010
type: SequenceAnnotation
persistent identity: annotation2398837
display id: annotation2398837
version: 1
top level: BBa_K1497013
location: https://synbiohub.org/public/igem/BBa_K1497013/annotation2398837/range2398837/1
component: BBa_K1497010

https://synbiohub.org/public/igem/BBa_K1497013/annotation2398837/range2398837/1
type: Range
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BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2398836
display id: annotation2398836
version: 1
top level: BBa_K1497013
location: https://synbiohub.org/public/igem/BBa_K1497013/annotation2398836/range2398836/1
component: BBa_B0034

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type: Range
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TerDnaE3(C
type: ComponentDefinition
persistent identity: BBa K1362131
display id: BBa_K1362131
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS + TerDnaE3 C-intein RFC[105] assembly construct (with His6)
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B??scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch??fer, Carolin Schmelas, Silvan Schmitz, Max Waldha
created: 2014-10-08T11:00:00Z
modified: 2015-05-08T01:10:05Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1738_
m user id: 0
owner id: 12377
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: ???
mutable notes: ???
mutable provenance: ???
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_G0000
component: BBa_K1362419
component: BBa_K1362431
component: BBa_K1362422
component: BBa_K1362418
component: BBa_K1362405
component: BBa_K1362465
component: BBa_K1362414
component: BBa_K1362467
component: BBa_K1362090
component: BBa_K1362421
sequence annotation: BBa_K1362419
sequence annotation: BBa_K1362465
sequence annotation: BBa_K1362422
sequence annotation: BBa_G0000
sequence annotation: BBa_K1362421
sequence annotation: BBa_K1362405
sequence annotation: BBa_K1362431
sequence annotation: BBa_K1362467
sequence annotation: BBa_K1362090
sequence annotation: BBa_K1362418
sequence annotation: BBa_K1362414
sequence: https://synbiohub.org/public/igem/BBa_K1362131_sequence/1

BBa_G0000
type: Component
persistent identity: component2410510
display id: component2410510
version: 1
top level: TerDnaE3(C
access: public
definition: https://synbiohub.org/public/igem/BBa_G0000/1

BBa_K1362419
type: Component
persistent identity: component2410518
display id: component2410518
version: 1
top level: TerDnaE3(C
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362419/1

BBa_K1362431
type: Component
persistent identity: component2410514
display id: component2410514
version: 1
top level: TerDnaE3(C
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362431/1

BBa_K1362422
type: Component
persistent identity: component2410539
display id: component2410539
version: 1
top level: TerDnaE3(C
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362422/1

BBa_K1362418
type: Component
persistent identity: component2410515
display id: component2410515
version: 1
top level: TerDnaE3(C
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362418/1

BBa_K1362405
type: Component
persistent identity: component2410517
display id: component2410517
version: 1
top level: TerDnaE3(C
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362405/1

BBa_K1362465
type: Component
persistent identity: component2410542
display id: component2410542
version: 1
top level: TerDnaE3(C
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362465/1

BBa_K1362414
type: Component
persistent identity: component2410511
display id: component2410511
version: 1
top level: TerDnaE3(C
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362414/1

BBa_K1362467
type: Component
persistent identity: component2410516
display id: component2410516
version: 1
top level: TerDnaE3(C
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362467/1

BBa_K1362090
type: Component
persistent identity: component2410509
display id: component2410509
version: 1
top level: TerDnaE3(C
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362090/1

BBa_K1362421
type: Component
persistent identity: component2410541
display id: component2410541
version: 1
top level: TerDnaE3(C
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362421/1

BBa_K1362419
type: SequenceAnnotation
persistent identity: annotation2410518
display id: annotation2410518
version: 1
top level: TerDnaE3(C
location: https://synbiohub.org/public/igem/BBa_K1362131/annotation2410518/range2410518/1
component: BBa_K1362419

https://synbiohub.org/public/igem/BBa_K1362131/annotation2410518/range2410518/1
type: Range
persistent identity: range2410518
display id: range2410518
version: 1
direction: direction/reverse
top level: TerDnaE3(C
start: 80
end: 83
orientation: inline

BBa_K1362465
type: SequenceAnnotation
persistent identity: annotation2410542
display id: annotation2410542
version: 1
top level: TerDnaE3(C
location: https://synbiohub.org/public/igem/BBa_K1362131/annotation2410542/range2410542/1
component: BBa_K1362465

https://synbiohub.org/public/igem/BBa_K1362131/annotation2410542/range2410542/1
type: Range
persistent identity: range2410542
display id: range2410542
version: 1
direction: direction/reverse
top level: TerDnaE3(C
start: 1171
end: 1172
orientation: inline

BBa_K1362422
type: SequenceAnnotation
persistent identity: annotation2410539
display id: annotation2410539
version: 1
top level: TerDnaE3(C
location: https://synbiohub.org/public/igem/BBa_K1362131/annotation2410539/range2410539/1
component: BBa_K1362422

https://synbiohub.org/public/igem/BBa_K1362131/annotation2410539/range2410539/1
type: Range
persistent identity: range2410539
display id: range2410539
version: 1
direction: direction/reverse
top level: TerDnaE3(C
start: 84
end: 1166
orientation: inline

BBa_G0000
type: SequenceAnnotation
persistent identity: annotation2410510
display id: annotation2410510
version: 1
top level: TerDnaE3(C
location: https://synbiohub.org/public/igem/BBa_K1362131/annotation2410510/range2410510/1
component: BBa_G0000

https://synbiohub.org/public/igem/BBa_K1362131/annotation2410510/range2410510/1
type: Range
persistent identity: range2410510
display id: range2410510
version: 1
direction: direction/reverse
top level: TerDnaE3(C
start: 30
end: 35
orientation: inline

BBa_K1362421
type: SequenceAnnotation
persistent identity: annotation2410541
display id: annotation2410541
version: 1
top level: TerDnaE3(C
location: https://synbiohub.org/public/igem/BBa_K1362131/annotation2410541/range2410541/1
component: BBa_K1362421

https://synbiohub.org/public/igem/BBa_K1362131/annotation2410541/range2410541/1
type: Range
persistent identity: range2410541
display id: range2410541
version: 1
direction: direction/reverse
top level: TerDnaE3(C
start: 1167
end: 1170
orientation: inline

BBa_K1362405
type: SequenceAnnotation
persistent identity: annotation2410517
display id: annotation2410517
version: 1
top level: TerDnaE3(C
location: https://synbiohub.org/public/igem/BBa_K1362131/annotation2410517/range2410517/1
component: BBa_K1362405

https://synbiohub.org/public/igem/BBa_K1362131/annotation2410517/range2410517/1
type: Range
persistent identity: range2410517
display id: range2410517
version: 1
direction: direction/reverse
top level: TerDnaE3(C
start: 66
end: 79
orientation: inline

BBa_K1362431
type: SequenceAnnotation
persistent identity: annotation2410514
display id: annotation2410514
version: 1
top level: TerDnaE3(C
location: https://synbiohub.org/public/igem/BBa_K1362131/annotation2410514/range2410514/1
component: BBa_K1362431

https://synbiohub.org/public/igem/BBa_K1362131/annotation2410514/range2410514/1
type: Range
persistent identity: range2410514
display id: range2410514
version: 1
direction: direction/reverse
top level: TerDnaE3(C
start: 39
end: 59
orientation: inline

BBa_K1362467
type: SequenceAnnotation
persistent identity: annotation2410516
display id: annotation2410516
version: 1
top level: TerDnaE3(C
location: https://synbiohub.org/public/igem/BBa_K1362131/annotation2410516/range2410516/1
component: BBa_K1362467

https://synbiohub.org/public/igem/BBa_K1362131/annotation2410516/range2410516/1
type: Range
persistent identity: range2410516
display id: range2410516
version: 1
direction: direction/reverse
top level: TerDnaE3(C
start: 64
end: 65
orientation: inline

BBa_K1362090
type: SequenceAnnotation
persistent identity: annotation2410509
display id: annotation2410509
version: 1
top level: TerDnaE3(C
location: https://synbiohub.org/public/igem/BBa_K1362131/annotation2410509/range2410509/1
component: BBa_K1362090

https://synbiohub.org/public/igem/BBa_K1362131/annotation2410509/range2410509/1
type: Range
persistent identity: range2410509
display id: range2410509
version: 1
direction: direction/reverse
top level: TerDnaE3(C
start: 1
end: 29
orientation: inline

BBa_K1362418
type: SequenceAnnotation
persistent identity: annotation2410515
display id: annotation2410515
version: 1
top level: TerDnaE3(C
location: https://synbiohub.org/public/igem/BBa_K1362131/annotation2410515/range2410515/1
component: BBa_K1362418

https://synbiohub.org/public/igem/BBa_K1362131/annotation2410515/range2410515/1
type: Range
persistent identity: range2410515
display id: range2410515
version: 1
direction: direction/reverse
top level: TerDnaE3(C
start: 60
end: 63
orientation: inline

BBa_K1362414
type: SequenceAnnotation
persistent identity: annotation2410511
display id: annotation2410511
version: 1
top level: TerDnaE3(C
location: https://synbiohub.org/public/igem/BBa_K1362131/annotation2410511/range2410511/1
component: BBa_K1362414

https://synbiohub.org/public/igem/BBa_K1362131/annotation2410511/range2410511/1
type: Range
persistent identity: range2410511
display id: range2410511
version: 1
direction: direction/reverse
top level: TerDnaE3(C
start: 36
end: 38
orientation: inline

Smt3-mDNMT
type: ComponentDefinition
persistent identity: BBa K1362500
display id: BBa_K1362500
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Smt3-Murine DNMT1(731-1602) fusion protein (with His6)
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Büscher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schäfer, Carolin Schmelas, Silvan Schmitz, Max Waldha
created: 2014-10-07T11:00:00Z
modified: 2015-05-08T01:10:06Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1738_
m user id: 0
owner id: 22951
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: TODO
mutable provenance: * Smt3: ''Saccharomyces cerevisiae'' * DNMT1: ''Mus musculus''
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1362449
component: BBa_K1362448
component: BBa_K1362458
component: BBa_K1362450
component: BBa_K1362456
component: BBa_J70594
component: BBa_K1362429
component: BBa_K1362446
sequence annotation: BBa_K1362448
sequence annotation: BBa_K1362450
sequence annotation: BBa_K1362446
sequence annotation: BBa_J70594
sequence annotation: BBa_K1362458
sequence annotation: BBa_K1362456
sequence annotation: BBa_K1362449
sequence annotation: BBa_K1362429
sequence: https://synbiohub.org/public/igem/BBa_K1362500_sequence/1

BBa_K1362449
type: Component
persistent identity: component2410950
display id: component2410950
version: 1
top level: Smt3-mDNMT
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362449/1

BBa_K1362448
type: Component
persistent identity: component2410952
display id: component2410952
version: 1
top level: Smt3-mDNMT
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362448/1

BBa_K1362458
type: Component
persistent identity: component2410949
display id: component2410949
version: 1
top level: Smt3-mDNMT
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362458/1

BBa_K1362450
type: Component
persistent identity: component2410955
display id: component2410955
version: 1
top level: Smt3-mDNMT
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362450/1

BBa_K1362456
type: Component
persistent identity: component2410951
display id: component2410951
version: 1
top level: Smt3-mDNMT
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362456/1

BBa_J70594
type: Component
persistent identity: component2410957
display id: component2410957
version: 1
top level: Smt3-mDNMT
access: public
definition: https://synbiohub.org/public/igem/BBa_J70594/1

BBa_K1362429
type: Component
persistent identity: component2410954
display id: component2410954
version: 1
top level: Smt3-mDNMT
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362429/1

BBa_K1362446
type: Component
persistent identity: component2410948
display id: component2410948
version: 1
top level: Smt3-mDNMT
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362446/1

BBa_K1362448
type: SequenceAnnotation
persistent identity: annotation2410952
display id: annotation2410952
version: 1
top level: Smt3-mDNMT
location: https://synbiohub.org/public/igem/BBa_K1362500/annotation2410952/range2410952/1
component: BBa_K1362448

https://synbiohub.org/public/igem/BBa_K1362500/annotation2410952/range2410952/1
type: Range
persistent identity: range2410952
display id: range2410952
version: 1
direction: direction/reverse
top level: Smt3-mDNMT
start: 58
end: 72
orientation: inline

BBa_K1362450
type: SequenceAnnotation
persistent identity: annotation2410955
display id: annotation2410955
version: 1
top level: Smt3-mDNMT
location: https://synbiohub.org/public/igem/BBa_K1362500/annotation2410955/range2410955/1
component: BBa_K1362450

https://synbiohub.org/public/igem/BBa_K1362500/annotation2410955/range2410955/1
type: Range
persistent identity: range2410955
display id: range2410955
version: 1
direction: direction/reverse
top level: Smt3-mDNMT
start: 367
end: 2982
orientation: inline

BBa_K1362446
type: SequenceAnnotation
persistent identity: annotation2410948
display id: annotation2410948
version: 1
top level: Smt3-mDNMT
location: https://synbiohub.org/public/igem/BBa_K1362500/annotation2410948/range2410948/1
component: BBa_K1362446

https://synbiohub.org/public/igem/BBa_K1362500/annotation2410948/range2410948/1
type: Range
persistent identity: range2410948
display id: range2410948
version: 1
direction: direction/reverse
top level: Smt3-mDNMT
start: 1
end: 12
orientation: inline

BBa_J70594
type: SequenceAnnotation
persistent identity: annotation2410957
display id: annotation2410957
version: 1
top level: Smt3-mDNMT
location: https://synbiohub.org/public/igem/BBa_K1362500/annotation2410957/range2410957/1
component: BBa_J70594

https://synbiohub.org/public/igem/BBa_K1362500/annotation2410957/range2410957/1
type: Range
persistent identity: range2410957
display id: range2410957
version: 1
direction: direction/reverse
top level: Smt3-mDNMT
start: 2983
end: 2988
orientation: inline

BBa_K1362458
type: SequenceAnnotation
persistent identity: annotation2410949
display id: annotation2410949
version: 1
top level: Smt3-mDNMT
location: https://synbiohub.org/public/igem/BBa_K1362500/annotation2410949/range2410949/1
component: BBa_K1362458

https://synbiohub.org/public/igem/BBa_K1362500/annotation2410949/range2410949/1
type: Range
persistent identity: range2410949
display id: range2410949
version: 1
direction: direction/reverse
top level: Smt3-mDNMT
start: 13
end: 30
orientation: inline

BBa_K1362456
type: SequenceAnnotation
persistent identity: annotation2410951
display id: annotation2410951
version: 1
top level: Smt3-mDNMT
location: https://synbiohub.org/public/igem/BBa_K1362500/annotation2410951/range2410951/1
component: BBa_K1362456

https://synbiohub.org/public/igem/BBa_K1362500/annotation2410951/range2410951/1
type: Range
persistent identity: range2410951
display id: range2410951
version: 1
direction: direction/reverse
top level: Smt3-mDNMT
start: 40
end: 57
orientation: inline

BBa_K1362449
type: SequenceAnnotation
persistent identity: annotation2410950
display id: annotation2410950
version: 1
top level: Smt3-mDNMT
location: https://synbiohub.org/public/igem/BBa_K1362500/annotation2410950/range2410950/1
component: BBa_K1362449

https://synbiohub.org/public/igem/BBa_K1362500/annotation2410950/range2410950/1
type: Range
persistent identity: range2410950
display id: range2410950
version: 1
direction: direction/reverse
top level: Smt3-mDNMT
start: 31
end: 39
orientation: inline

BBa_K1362429
type: SequenceAnnotation
persistent identity: annotation2410954
display id: annotation2410954
version: 1
top level: Smt3-mDNMT
location: https://synbiohub.org/public/igem/BBa_K1362500/annotation2410954/range2410954/1
component: BBa_K1362429

https://synbiohub.org/public/igem/BBa_K1362500/annotation2410954/range2410954/1
type: Range
persistent identity: range2410954
display id: range2410954
version: 1
direction: direction/reverse
top level: Smt3-mDNMT
start: 73
end: 366
orientation: inline

BBa_K1497032
type: ComponentDefinition
persistent identity: BBa K1497032
display id: BBa_K1497032
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Scaffold (G-S-P-His-Cys)
creator: Sascha Hein, Rene Sahm, Malte Blumenroth, Tobias Gabriel, Sebastian Palluk
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:46Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1877_
m user id: 0
owner id: 12466
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Scaffold (G-S-P-His-Cys)
mutable notes: Scaffold (G-S-P-His-Cys)
mutable provenance: Scaffold (G-S-P-His-Cys)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1497032_sequence/1

BBa_K1321092
type: ComponentDefinition
persistent identity: BBa K1321092
display id: BBa_K1321092
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Phytochelatin (PC) EC20 fused to CBDcex
creator: Xenia Spencer-Milnes
created: 2014-10-06T11:00:00Z
modified: 2015-06-17T12:17:32Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 4206
owner id: 20780
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: PhytoC+CBD7. TBC
mutable notes: PhytoC+CBD7. TBC
mutable provenance: PhytoC+CBD7. TBC
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1321005
component: BBa_K1321003
component: BBa_B0105
sequence annotation: BBa_B0105
sequence annotation: BBa_K1321005
sequence annotation: BBa_K1321003
sequence: https://synbiohub.org/public/igem/BBa_K1321092_sequence/1

BBa_K1321005
type: Component
persistent identity: component2414618
display id: component2414618
version: 1
top level: BBa_K1321092
access: public
definition: BBa_K1321005

BBa_K1321003
type: Component
persistent identity: component2414621
display id: component2414621
version: 1
top level: BBa_K1321092
access: public
definition: https://synbiohub.org/public/igem/BBa_K1321003/1

BBa_B0105
type: Component
persistent identity: component2414620
display id: component2414620
version: 1
top level: BBa_K1321092
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2414620
display id: annotation2414620
version: 1
top level: BBa_K1321092
location: https://synbiohub.org/public/igem/BBa_K1321092/annotation2414620/range2414620/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_K1321092/annotation2414620/range2414620/1
type: Range
persistent identity: range2414620
display id: range2414620
version: 1
direction: direction/reverse
top level: BBa_K1321092
start: 124
end: 129
orientation: inline

BBa_K1321005
type: SequenceAnnotation
persistent identity: annotation2414618
display id: annotation2414618
version: 1
top level: BBa_K1321092
location: https://synbiohub.org/public/igem/BBa_K1321092/annotation2414618/range2414618/1
component: BBa_K1321005

https://synbiohub.org/public/igem/BBa_K1321092/annotation2414618/range2414618/1
type: Range
persistent identity: range2414618
display id: range2414618
version: 1
direction: direction/reverse
top level: BBa_K1321092
start: 1
end: 123
orientation: inline

BBa_K1321003
type: SequenceAnnotation
persistent identity: annotation2414621
display id: annotation2414621
version: 1
top level: BBa_K1321092
location: https://synbiohub.org/public/igem/BBa_K1321092/annotation2414621/range2414621/1
component: BBa_K1321003

https://synbiohub.org/public/igem/BBa_K1321092/annotation2414621/range2414621/1
type: Range
persistent identity: range2414621
display id: range2414621
version: 1
direction: direction/reverse
top level: BBa_K1321092
start: 130
end: 456
orientation: inline

BBa_K1491017
type: ComponentDefinition
persistent identity: BBa K1491017
display id: BBa_K1491017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Codon Optimized Supernova
creator: Danielle Peters
created: 2014-08-03T11:00:00Z
modified: 2015-05-08T01:10:44Z
discontinued: false
dominant: false
group u list: _1871_
m user id: 0
owner id: 22521
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Supernova is the monomeric version of KillerRed (BBa_K1184000). It has been codon optimized for E. coli.
mutable notes: Monomeric version of KillerRed
mutable provenance: The original KillerRed is BBa_K11840000.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: 6x His Tag
sequence: https://synbiohub.org/public/igem/BBa_K1491017_sequence/1

6x His Tag
type: SequenceAnnotation
persistent identity: annotation2380356
display id: annotation2380356
version: 1
top level: BBa_K1491017
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1491017/annotation2380356/range2380356/1

https://synbiohub.org/public/igem/BBa_K1491017/annotation2380356/range2380356/1
type: Range
persistent identity: range2380356
display id: range2380356
version: 1
direction: direction/forward
top level: BBa_K1491017
start: 718
end: 735
orientation: inline

BBa_K1334002
type: ComponentDefinition
persistent identity: BBa K1334002
display id: BBa_K1334002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: HxlR+P-formaldehyde
creator: Haoyuan Sun
created: 2014-09-26T11:00:00Z
modified: 2015-05-08T01:09:58Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1709_
m user id: 0
owner id: 22430
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: A right promoter which can sense formaldehyde while there is a left promoter which can erpress the HxlR protein theoretically.The HxlR protein can enhance the function of the P-formaldehyde.
mutable notes: The start codon of the HxlR protein is UUG.
mutable provenance: We get this part from Bacillus subtilis 168.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: HxlR coding sequence
sequence annotation: rbs
sequence annotation: Formaldehyde promoter
sequence annotation: weak promoter
sequence annotation: HxlR protein binding site 2
sequence annotation: HxlR protein binding site 1
sequence: https://synbiohub.org/public/igem/BBa_K1334002_sequence/1

HxlR coding sequence
type: SequenceAnnotation
persistent identity: annotation2387313
display id: annotation2387313
version: 1
top level: BBa_K1334002
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1334002/annotation2387313/range2387313/1

https://synbiohub.org/public/igem/BBa_K1334002/annotation2387313/range2387313/1
type: Range
persistent identity: range2387313
display id: range2387313
version: 1
direction: direction/reverse
top level: BBa_K1334002
start: 1
end: 363
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2387242
display id: annotation2387242
version: 1
top level: BBa_K1334002
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1334002/annotation2387242/range2387242/1

https://synbiohub.org/public/igem/BBa_K1334002/annotation2387242/range2387242/1
type: Range
persistent identity: range2387242
display id: range2387242
version: 1
direction: direction/forward
top level: BBa_K1334002
start: 579
end: 586
orientation: inline

Formaldehyde promoter
type: SequenceAnnotation
persistent identity: annotation2387265
display id: annotation2387265
version: 1
top level: BBa_K1334002
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1334002/annotation2387265/range2387265/1

https://synbiohub.org/public/igem/BBa_K1334002/annotation2387265/range2387265/1
type: Range
persistent identity: range2387265
display id: range2387265
version: 1
direction: direction/forward
top level: BBa_K1334002
start: 534
end: 570
orientation: inline

weak promoter
type: SequenceAnnotation
persistent identity: annotation2387310
display id: annotation2387310
version: 1
top level: BBa_K1334002
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1334002/annotation2387310/range2387310/1

https://synbiohub.org/public/igem/BBa_K1334002/annotation2387310/range2387310/1
type: Range
persistent identity: range2387310
display id: range2387310
version: 1
direction: direction/reverse
top level: BBa_K1334002
start: 422
end: 470
orientation: inline

HxlR protein binding site 2
type: SequenceAnnotation
persistent identity: annotation2387311
display id: annotation2387311
version: 1
top level: BBa_K1334002
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1334002/annotation2387311/range2387311/1

https://synbiohub.org/public/igem/BBa_K1334002/annotation2387311/range2387311/1
type: Range
persistent identity: range2387311
display id: range2387311
version: 1
direction: direction/forward
top level: BBa_K1334002
start: 514
end: 538
orientation: inline

HxlR protein binding site 1
type: SequenceAnnotation
persistent identity: annotation2387312
display id: annotation2387312
version: 1
top level: BBa_K1334002
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1334002/annotation2387312/range2387312/1

https://synbiohub.org/public/igem/BBa_K1334002/annotation2387312/range2387312/1
type: Range
persistent identity: range2387312
display id: range2387312
version: 1
direction: direction/forward
top level: BBa_K1334002
start: 489
end: 513
orientation: inline

BBa_K1315005
type: ComponentDefinition
persistent identity: BBa K1315005
display id: BBa_K1315005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: The clp gene from Xanthomonas campestris
creator: Dimitrios Michailidis
created: 2014-09-24T11:00:00Z
modified: 2015-05-08T01:09:50Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1690_
m user id: 0
owner id: 21812
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: c-di-GMP Repressed DNA Binding Protein (Clp) Quorum sensing and bacterial cell signalling are mechanisms known to be utilised by bacteria to regulate their virulence. The RpfC-RpfG sensory system has been found to activate the production of virulence factors like extracellular enzymes and polysaccharides in Xanthomonas campestris. It has been also suggested that this system is involved in linking environmental stimuli, including DSF, with pathogenicity in Xanthomonas campestris. DSF is a cis-unsaturated fatty acid from the DSF family. The RpfC-RpfG two-component system is the most well understood one and is present in all Xanthomonads. http://www.ncbi.nlm.nih.gov/pubmed/?term=r+ryan+dsf+family http://www.ncbi.nlm.nih.gov/pubmed/?term=A+two-component+system+involving+an+HD-GYP+domain+protein+links+cell%E2%80%93cell+signalling+to+pathogenicity+gene+expression+in+Xanthomonas+campestris
mutable notes: The sequence had 1 PstI site that was mutated out by site-directed mutagenesis.
mutable provenance: The source of this part is Xanthomonas campestris pv. campestris 8004 genomic DNA which was provided by the Robert Ryan lab in the Molecular Microbiology department of the University of Dundee, UK.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Clp
sequence: https://synbiohub.org/public/igem/BBa_K1315005_sequence/1

Clp
type: SequenceAnnotation
persistent identity: annotation2386527
display id: annotation2386527
version: 1
top level: BBa_K1315005
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1315005/annotation2386527/range2386527/1

https://synbiohub.org/public/igem/BBa_K1315005/annotation2386527/range2386527/1
type: Range
persistent identity: range2386527
display id: range2386527
version: 1
direction: direction/forward
top level: BBa_K1315005
start: 1
end: 696
orientation: inline

BBa_K1602014
type: ComponentDefinition
persistent identity: BBa K1602014
display id: BBa_K1602014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0034-yagE
creator: Leon Kraus, Jonathan Pletzer-Zelgert, Jonas Sindlinger, Carmen Sakas Gandullo, Sebastian Holzner, Elena Tonchevska, Mirjam Haun
created: 2015-09-15T11:00:00Z
modified: 2015-09-18T07:26:18Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2019_
m user id: 25990
owner id: 22022
owning group id: 9
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_K1602011
component: BBa_B0034
sequence annotation: BBa_K1602011
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K1602014_sequence/1

BBa_K1602011
type: Component
persistent identity: component2462504
display id: component2462504
version: 1
top level: BBa_K1602014
access: public
definition: BBa_K1602011

BBa_B0034
type: Component
persistent identity: component2462503
display id: component2462503
version: 1
top level: BBa_K1602014
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1602011
type: SequenceAnnotation
persistent identity: annotation2462504
display id: annotation2462504
version: 1
top level: BBa_K1602014
location: https://synbiohub.org/public/igem/BBa_K1602014/annotation2462504/range2462504/1
component: BBa_K1602011

https://synbiohub.org/public/igem/BBa_K1602014/annotation2462504/range2462504/1
type: Range
persistent identity: range2462504
display id: range2462504
version: 1
direction: direction/reverse
top level: BBa_K1602014
start: 19
end: 927
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2462503
display id: annotation2462503
version: 1
top level: BBa_K1602014
location: https://synbiohub.org/public/igem/BBa_K1602014/annotation2462503/range2462503/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1602014/annotation2462503/range2462503/1
type: Range
persistent identity: range2462503
display id: range2462503
version: 1
direction: direction/reverse
top level: BBa_K1602014
start: 1
end: 12
orientation: inline

BBa_K1315008
type: ComponentDefinition
persistent identity: BBa K1315008
display id: BBa_K1315008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: The cblD promoter from Burkholderia
creator: Dimitrios Michailidis
created: 2014-09-25T11:00:00Z
modified: 2015-05-08T01:09:50Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1690_
m user id: 0
owner id: 21812
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is a promoter that is activated by the OmpR-like two-component response regulator BCAM0228 (Bba_K1315007) in the BCAM0227-BCAM0228 sensor system. The Burkholderia diffusible signal (BDSF) is being picked up by the BCAM0227 sensor kinase that is hypothesised to eventually lead in involvement of BCAM0228. BCAM0228 is then activating cblD.
mutable notes: The sequence did not have any restriction sites to remove so it was immediately PCRed out of the genome and had the registry prefix and suffix added to it. It was then ligated into the pSB1C3 vector
mutable provenance: The source of this part is Burkholderia cenocepacia J2315 genomic DNA which was provided by the Robert Ryan lab in the Molecular Microbiology department of the University of Dundee, UK.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: cblD
sequence: https://synbiohub.org/public/igem/BBa_K1315008_sequence/1

cblD
type: SequenceAnnotation
persistent identity: annotation2387049
display id: annotation2387049
version: 1
top level: BBa_K1315008
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1315008/annotation2387049/range2387049/1

https://synbiohub.org/public/igem/BBa_K1315008/annotation2387049/range2387049/1
type: Range
persistent identity: range2387049
display id: range2387049
version: 1
direction: direction/forward
top level: BBa_K1315008
start: 1
end: 250
orientation: inline

BBa_K1689017
type: ComponentDefinition
persistent identity: BBa K1689017
display id: BBa_K1689017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: F3-dCas9
creator: Wei weijia
created: 2015-09-15T11:00:00Z
modified: 2015-09-27T01:38:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2107_
m user id: 26531
owner id: 26531
owning group id: 9
status: status/Planning
bookmark: false
mutable description: pSB1C3-6H-F3-dCas9, fusion protein of F3 segment of DHFR fused with dCas9, the His tag facilitates protein purification
mutable notes: The coding sequence of this fusion protein is too long, you'd better amplify is separately.
mutable provenance: E.coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: His tag
sequence annotation: dCas9
sequence annotation: F3-dCas9
sequence annotation: start
sequence annotation: random spacer
sequence annotation: stop
sequence annotation: F3
sequence annotation: GGGGS*2 Linker
sequence: https://synbiohub.org/public/igem/BBa_K1689017_sequence/1

His tag
type: SequenceAnnotation
persistent identity: annotation2469977
display id: annotation2469977
version: 1
top level: BBa_K1689017
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1689017/annotation2469977/range2469977/1

https://synbiohub.org/public/igem/BBa_K1689017/annotation2469977/range2469977/1
type: Range
persistent identity: range2469977
display id: range2469977
version: 1
direction: direction/forward
top level: BBa_K1689017
start: 37
end: 54
orientation: inline

dCas9
type: SequenceAnnotation
persistent identity: annotation2469982
display id: annotation2469982
version: 1
top level: BBa_K1689017
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1689017/annotation2469982/range2469982/1

https://synbiohub.org/public/igem/BBa_K1689017/annotation2469982/range2469982/1
type: Range
persistent identity: range2469982
display id: range2469982
version: 1
direction: direction/forward
top level: BBa_K1689017
start: 298
end: 4398
orientation: inline

F3-dCas9
type: SequenceAnnotation
persistent identity: annotation2469975
display id: annotation2469975
version: 1
top level: BBa_K1689017
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1689017/annotation2469975/range2469975/1

https://synbiohub.org/public/igem/BBa_K1689017/annotation2469975/range2469975/1
type: Range
persistent identity: range2469975
display id: range2469975
version: 1
direction: direction/forward
top level: BBa_K1689017
start: 34
end: 4401
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2477790
display id: annotation2477790
version: 1
top level: BBa_K1689017
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1689017/annotation2477790/range2477790/1

https://synbiohub.org/public/igem/BBa_K1689017/annotation2477790/range2477790/1
type: Range
persistent identity: range2477790
display id: range2477790
version: 1
direction: direction/forward
top level: BBa_K1689017
start: 34
end: 36
orientation: inline

random spacer
type: SequenceAnnotation
persistent identity: annotation2477789
display id: annotation2477789
version: 1
top level: BBa_K1689017
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1689017/annotation2477789/range2477789/1

https://synbiohub.org/public/igem/BBa_K1689017/annotation2477789/range2477789/1
type: Range
persistent identity: range2477789
display id: range2477789
version: 1
direction: direction/forward
top level: BBa_K1689017
start: 1
end: 33
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2477791
display id: annotation2477791
version: 1
top level: BBa_K1689017
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1689017/annotation2477791/range2477791/1

https://synbiohub.org/public/igem/BBa_K1689017/annotation2477791/range2477791/1
type: Range
persistent identity: range2477791
display id: range2477791
version: 1
direction: direction/forward
top level: BBa_K1689017
start: 4398
end: 4401
orientation: inline

F3
type: SequenceAnnotation
persistent identity: annotation2469978
display id: annotation2469978
version: 1
top level: BBa_K1689017
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1689017/annotation2469978/range2469978/1

https://synbiohub.org/public/igem/BBa_K1689017/annotation2469978/range2469978/1
type: Range
persistent identity: range2469978
display id: range2469978
version: 1
direction: direction/forward
top level: BBa_K1689017
start: 55
end: 267
orientation: inline

GGGGS*2 Linker
type: SequenceAnnotation
persistent identity: annotation2469979
display id: annotation2469979
version: 1
top level: BBa_K1689017
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1689017/annotation2469979/range2469979/1

https://synbiohub.org/public/igem/BBa_K1689017/annotation2469979/range2469979/1
type: Range
persistent identity: range2469979
display id: range2469979
version: 1
direction: direction/forward
top level: BBa_K1689017
start: 268
end: 297
orientation: inline

random spacer
type: SequenceAnnotation
persistent identity: annotation2477792
display id: annotation2477792
version: 1
top level: BBa_K1689017
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1689017/annotation2477792/range2477792/1

https://synbiohub.org/public/igem/BBa_K1689017/annotation2477792/range2477792/1
type: Range
persistent identity: range2477792
display id: range2477792
version: 1
direction: direction/forward
top level: BBa_K1689017
start: 4402
end: 4472
orientation: inline

HIC genera
type: ComponentDefinition
persistent identity: BBa K1602020
display id: BBa_K1602020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Inducible generator of Humicola insolens cutinase (HIC) with N-terminal His-Tag
creator: Sebastian Barthel, Steven Pilger, Jonas Sindlinger, Bianca Reisinger, Sven Jager
created: 2015-09-15T11:00:00Z
modified: 2015-09-26T03:05:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2019_
m user id: 12512
owner id: 22022
owning group id: 9
status: status/Planning
bookmark: true
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K1602018
component: BBa_I719005
component: BBa_B0034
sequence annotation: BBa_I719005
sequence annotation: BBa_K1602018
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K1602020_sequence/1

BBa_K1602018
type: Component
persistent identity: component2462409
display id: component2462409
version: 1
top level: HIC genera
access: public
definition: https://synbiohub.org/public/igem/BBa_K1602018/1

BBa_I719005
type: Component
persistent identity: component2462406
display id: component2462406
version: 1
top level: HIC genera
access: public
definition: https://synbiohub.org/public/igem/BBa_I719005/1

BBa_B0034
type: Component
persistent identity: component2462408
display id: component2462408
version: 1
top level: HIC genera
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_I719005
type: SequenceAnnotation
persistent identity: annotation2462406
display id: annotation2462406
version: 1
top level: HIC genera
location: https://synbiohub.org/public/igem/BBa_K1602020/annotation2462406/range2462406/1
component: BBa_I719005

https://synbiohub.org/public/igem/BBa_K1602020/annotation2462406/range2462406/1
type: Range
persistent identity: range2462406
display id: range2462406
version: 1
direction: direction/reverse
top level: HIC genera
start: 1
end: 23
orientation: inline

BBa_K1602018
type: SequenceAnnotation
persistent identity: annotation2462409
display id: annotation2462409
version: 1
top level: HIC genera
location: https://synbiohub.org/public/igem/BBa_K1602020/annotation2462409/range2462409/1
component: BBa_K1602018

https://synbiohub.org/public/igem/BBa_K1602020/annotation2462409/range2462409/1
type: Range
persistent identity: range2462409
display id: range2462409
version: 1
direction: direction/reverse
top level: HIC genera
start: 50
end: 652
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2462408
display id: annotation2462408
version: 1
top level: HIC genera
location: https://synbiohub.org/public/igem/BBa_K1602020/annotation2462408/range2462408/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1602020/annotation2462408/range2462408/1
type: Range
persistent identity: range2462408
display id: range2462408
version: 1
direction: direction/reverse
top level: HIC genera
start: 32
end: 43
orientation: inline

BBa_K1639001
type: ComponentDefinition
persistent identity: BBa K1639001
display id: BBa_K1639001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Trigger RNA
creator: Mustafa Yılmaz
created: 2015-09-11T11:00:00Z
modified: 2015-09-18T06:50:31Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2056_
m user id: 20835
owner id: 20835
owning group id: 9
status: status/Planning
bookmark: true
mutable description: This part required for producing reporter flourescence protein GFP from Toehold-GFP(BBa_K1639000) composite part.
mutable notes: This part synthetase with strong T7 Promoter downstream of Trigger RNA. There is also a T7 Terminator after Trigger RNA.
mutable provenance: Wyss Institute for Biologically Inspired Engineering. Sequence is taken from supplementary data of article: "Toehold Switches: De-Novo-Designed Regulators of Gene Expression". (PMID: 25417166)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence annotation: T7 Promoter
sequence annotation: T7 Terminator
sequence annotation: Trigger RNA
sequence: https://synbiohub.org/public/igem/BBa_K1639001_sequence/1

T7 Promoter
type: SequenceAnnotation
persistent identity: annotation2452874
display id: annotation2452874
version: 1
top level: BBa_K1639001
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1639001/annotation2452874/range2452874/1

https://synbiohub.org/public/igem/BBa_K1639001/annotation2452874/range2452874/1
type: Range
persistent identity: range2452874
display id: range2452874
version: 1
direction: direction/forward
top level: BBa_K1639001
start: 1
end: 19
orientation: inline

T7 Terminator
type: SequenceAnnotation
persistent identity: annotation2452876
display id: annotation2452876
version: 1
top level: BBa_K1639001
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1639001/annotation2452876/range2452876/1

https://synbiohub.org/public/igem/BBa_K1639001/annotation2452876/range2452876/1
type: Range
persistent identity: range2452876
display id: range2452876
version: 1
direction: direction/forward
top level: BBa_K1639001
start: 84
end: 130
orientation: inline

Trigger RNA
type: SequenceAnnotation
persistent identity: annotation2452875
display id: annotation2452875
version: 1
top level: BBa_K1639001
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1639001/annotation2452875/range2452875/1

https://synbiohub.org/public/igem/BBa_K1639001/annotation2452875/range2452875/1
type: Range
persistent identity: range2452875
display id: range2452875
version: 1
direction: direction/forward
top level: BBa_K1639001
start: 22
end: 83
orientation: inline

BBa_K1321348
type: ComponentDefinition
persistent identity: BBa K1321348
display id: BBa_K1321348
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: sfGFP fused to dCBD with linker in RFC 25
creator: Chris N Micklem
created: 2014-10-07T11:00:00Z
modified: 2015-06-17T12:30:41Z
discontinued: false
dominant: false
group u list: _1696_
m user id: 4206
owner id: 20830
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: asdf
mutable notes: asdf
mutable provenance: asdf
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1321337
component: BBa_B0105
component: BBa_K1321340
sequence annotation: BBa_K1321340
sequence annotation: BBa_K1321337
sequence annotation: BBa_B0105
sequence: https://synbiohub.org/public/igem/BBa_K1321348_sequence/1

BBa_K1321337
type: Component
persistent identity: component2415136
display id: component2415136
version: 1
top level: BBa_K1321348
access: public
definition: BBa_K1321337

BBa_B0105
type: Component
persistent identity: component2415138
display id: component2415138
version: 1
top level: BBa_K1321348
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_K1321340
type: Component
persistent identity: component2415140
display id: component2415140
version: 1
top level: BBa_K1321348
access: public
definition: BBa_K1321340

BBa_K1321340
type: SequenceAnnotation
persistent identity: annotation2415140
display id: annotation2415140
version: 1
top level: BBa_K1321348
location: https://synbiohub.org/public/igem/BBa_K1321348/annotation2415140/range2415140/1
component: BBa_K1321340

https://synbiohub.org/public/igem/BBa_K1321348/annotation2415140/range2415140/1
type: Range
persistent identity: range2415140
display id: range2415140
version: 1
direction: direction/reverse
top level: BBa_K1321348
start: 718
end: 1083
orientation: inline

BBa_K1321337
type: SequenceAnnotation
persistent identity: annotation2415136
display id: annotation2415136
version: 1
top level: BBa_K1321348
location: https://synbiohub.org/public/igem/BBa_K1321348/annotation2415136/range2415136/1
component: BBa_K1321337

https://synbiohub.org/public/igem/BBa_K1321348/annotation2415136/range2415136/1
type: Range
persistent identity: range2415136
display id: range2415136
version: 1
direction: direction/reverse
top level: BBa_K1321348
start: 1
end: 711
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2415138
display id: annotation2415138
version: 1
top level: BBa_K1321348
location: https://synbiohub.org/public/igem/BBa_K1321348/annotation2415138/range2415138/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_K1321348/annotation2415138/range2415138/1
type: Range
persistent identity: range2415138
display id: range2415138
version: 1
direction: direction/reverse
top level: BBa_K1321348
start: 712
end: 717
orientation: inline

BBa_K1585211
type: ComponentDefinition
persistent identity: BBa K1585211
display id: BBa_K1585211
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0034.HPS
creator: Laura Grabowski
created: 2015-08-24T11:00:00Z
modified: 2015-08-28T08:21:36Z
discontinued: false
dominant: false
group u list: _2002_
m user id: 19522
owner id: 24505
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This is the translational unit of 3-hexulose-6-phosphate synthase (HPS) from Bacillus methanolicus MGA3. It is codon optimized for E.coli.
mutable notes: RFC10 compatible
mutable provenance: Bacillus methanolicus MGA3
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1585201
component: BBa_B0034
sequence annotation: BBa_K1585201
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K1585211_sequence/1

BBa_K1585201
type: Component
persistent identity: component2439453
display id: component2439453
version: 1
top level: BBa_K1585211
access: public
definition: https://synbiohub.org/public/igem/BBa_K1585201/1

BBa_B0034
type: Component
persistent identity: component2439452
display id: component2439452
version: 1
top level: BBa_K1585211
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1585201
type: SequenceAnnotation
persistent identity: annotation2439453
display id: annotation2439453
version: 1
top level: BBa_K1585211
location: https://synbiohub.org/public/igem/BBa_K1585211/annotation2439453/range2439453/1
component: BBa_K1585201

https://synbiohub.org/public/igem/BBa_K1585211/annotation2439453/range2439453/1
type: Range
persistent identity: range2439453
display id: range2439453
version: 1
direction: direction/reverse
top level: BBa_K1585211
start: 19
end: 657
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2439452
display id: annotation2439452
version: 1
top level: BBa_K1585211
location: https://synbiohub.org/public/igem/BBa_K1585211/annotation2439452/range2439452/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1585211/annotation2439452/range2439452/1
type: Range
persistent identity: range2439452
display id: range2439452
version: 1
direction: direction/reverse
top level: BBa_K1585211
start: 1
end: 12
orientation: inline

BBa_K1497009
type: ComponentDefinition
persistent identity: BBa K1497009
display id: BBa_K1497009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: F3H
creator: Bastian Wagner, Christian Mende, Andreas Schmidt, Max Dombrowsky
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1877_
m user id: 0
owner id: 12466
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: d
mutable notes: d
mutable provenance: d
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1497009_sequence/1

BBa_K1509000
type: ComponentDefinition
persistent identity: BBa K1509000
display id: BBa_K1509000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Coding for the trans-acting regulator SmtB
creator: Tong Li
created: 2014-09-25T11:00:00Z
modified: 2015-07-23T03:26:04Z
discontinued: false
dominant: false
group u list: _1889_
m user id: 4206
owner id: 24032
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: In 1993, Huckle and co-workers reported the isolation and analysis of the smt locus from Synechococcus sp.. Gene smtB, one of the components of the locus, encodes a transcriptional repressor of gene smtA, with which is required for normal tolerance to Zn2+ and Cd2+. The product of smtB functions by binding to the smt operator-promoter(smtO-P) and dissociating in the presence of Zn2+ due to the classical helix-turn-helix motif similar to many DNA-binding proteins. It can also be genomically rearranged when induced by Cd2+, leading to a deletion within smtB because of a highly iterated palindrome (HIP1), a highly represented octanucleotides(5′-GCGATC-GC-3′) which traverse both borders of the excised element. These two mechanisms both cause the elevated expression of smtA.
mutable notes: According to the mechanisms of smt locus represented above, gene smtB should function with the accompany of smt operator-promoter. Previous cases selected smtA whose product functions by encoding MT-like to sequester metal ions, while our case intended to take advantage of the trigger lies in this locus. By imitating former researches, in which a promoter less lacZ gene was fused upstream of smtA, we respectively splice two selected reporter genes and sequence from smt operator-promoter to smtB, equivalent to replacing smtA with reporter genes, to visualize the effects of metal ions on the locus.
mutable provenance: Metallothioneins(MTs), discovered in horse kidney in 1957 by Margoshes and Vallee are identified as low-molecular weight and sulphydryl rich proteins which bind metal ions in metal-thiolate clusters and whose synthesis increases in response to elevated concentrations of certain metals. In 1988, Olafson and co-workers reported the amino acid sequence of a metal-associated protein isolated from cyanobacterium Synechococcus sp.. With the function of binding metal ions remaining, it only has less than 20% homology in amino acid sequence with known MTs in eukaryotes and thus we call it class II MT or on some occasions MT-like. Owing to the works of Robinson and Huckle, the corresponding locus of Synechococcus PCC7942, smt locus was isolated and analyzed. It includes smtA which encodes the class II MT and a divergently transcribed gene smtB which encodes a trans-acting, autoregulatory repressor that exerts metal ion-inducible negative control over smtA transcription. Between them lies a 100-bp operator-promoter region(smt O-P, BBa_K1509001), which is responsible for the divergent orientation of smtA and smtB.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: smtB
sequence: https://synbiohub.org/public/igem/BBa_K1509000_sequence/1

smtB
type: SequenceAnnotation
persistent identity: annotation2387081
display id: annotation2387081
version: 1
top level: BBa_K1509000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1509000/annotation2387081/range2387081/1

https://synbiohub.org/public/igem/BBa_K1509000/annotation2387081/range2387081/1
type: Range
persistent identity: range2387081
display id: range2387081
version: 1
direction: direction/forward
top level: BBa_K1509000
start: 1
end: 301
orientation: inline

BBa_K1850013
type: ComponentDefinition
persistent identity: BBa K1850013
display id: BBa_K1850013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pAra - fim
creator: Lydia Goldberg
created: 2015-09-14T11:00:00Z
modified: 2015-09-16T11:00:54Z
discontinued: false
dominant: false
group u list: _2277_
m user id: 26421
owner id: 26421
owning group id: 9
status: status/Planning
bookmark: false
mutable description: placeholder
mutable notes: placeholder
mutable provenance: placeholder
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: fimI
sequence annotation: fimA
sequence annotation: pAra
sequence annotation: fimC
sequence annotation: fimG
sequence annotation: fimF
sequence annotation: fimD
sequence: https://synbiohub.org/public/igem/BBa_K1850013_sequence/1

fimI
type: SequenceAnnotation
persistent identity: annotation2463811
display id: annotation2463811
version: 1
top level: BBa_K1850013
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1850013/annotation2463811/range2463811/1

https://synbiohub.org/public/igem/BBa_K1850013/annotation2463811/range2463811/1
type: Range
persistent identity: range2463811
display id: range2463811
version: 1
direction: direction/forward
top level: BBa_K1850013
start: 814
end: 1353
orientation: inline

fimA
type: SequenceAnnotation
persistent identity: annotation2463809
display id: annotation2463809
version: 1
top level: BBa_K1850013
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1850013/annotation2463809/range2463809/1

https://synbiohub.org/public/igem/BBa_K1850013/annotation2463809/range2463809/1
type: Range
persistent identity: range2463809
display id: range2463809
version: 1
direction: direction/forward
top level: BBa_K1850013
start: 201
end: 749
orientation: inline

pAra
type: SequenceAnnotation
persistent identity: annotation2463810
display id: annotation2463810
version: 1
top level: BBa_K1850013
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1850013/annotation2463810/range2463810/1

https://synbiohub.org/public/igem/BBa_K1850013/annotation2463810/range2463810/1
type: Range
persistent identity: range2463810
display id: range2463810
version: 1
direction: direction/forward
top level: BBa_K1850013
start: 1
end: 130
orientation: inline

fimC
type: SequenceAnnotation
persistent identity: annotation2463812
display id: annotation2463812
version: 1
top level: BBa_K1850013
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1850013/annotation2463812/range2463812/1

https://synbiohub.org/public/igem/BBa_K1850013/annotation2463812/range2463812/1
type: Range
persistent identity: range2463812
display id: range2463812
version: 1
direction: direction/forward
top level: BBa_K1850013
start: 1390
end: 2115
orientation: inline

fimG
type: SequenceAnnotation
persistent identity: annotation2463816
display id: annotation2463816
version: 1
top level: BBa_K1850013
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1850013/annotation2463816/range2463816/1

https://synbiohub.org/public/igem/BBa_K1850013/annotation2463816/range2463816/1
type: Range
persistent identity: range2463816
display id: range2463816
version: 1
direction: direction/forward
top level: BBa_K1850013
start: 5371
end: 5874
orientation: inline

fimF
type: SequenceAnnotation
persistent identity: annotation2463815
display id: annotation2463815
version: 1
top level: BBa_K1850013
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1850013/annotation2463815/range2463815/1

https://synbiohub.org/public/igem/BBa_K1850013/annotation2463815/range2463815/1
type: Range
persistent identity: range2463815
display id: range2463815
version: 1
direction: direction/forward
top level: BBa_K1850013
start: 4828
end: 5358
orientation: inline

fimD
type: SequenceAnnotation
persistent identity: annotation2463813
display id: annotation2463813
version: 1
top level: BBa_K1850013
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1850013/annotation2463813/range2463813/1

https://synbiohub.org/public/igem/BBa_K1850013/annotation2463813/range2463813/1
type: Range
persistent identity: range2463813
display id: range2463813
version: 1
direction: direction/forward
top level: BBa_K1850013
start: 2182
end: 4818
orientation: inline

BBa_K1321343
type: ComponentDefinition
persistent identity: BBa K1321343
display id: BBa_K1321343
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NiBP fused to CBDclos in RFC 25
creator: Chris N Micklem
created: 2014-10-07T11:00:00Z
modified: 2015-06-17T12:26:16Z
discontinued: false
dominant: false
group u list: _1696_
m user id: 4206
owner id: 20830
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: adsdf
mutable notes: asdf
mutable provenance: asdf
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1321009
component: BBa_B0105
component: BBa_K1321002
sequence annotation: BBa_K1321009
sequence annotation: BBa_B0105
sequence annotation: BBa_K1321002
sequence: https://synbiohub.org/public/igem/BBa_K1321343_sequence/1

BBa_K1321009
type: Component
persistent identity: component2415098
display id: component2415098
version: 1
top level: BBa_K1321343
access: public
definition: BBa_K1321009

BBa_B0105
type: Component
persistent identity: component2415100
display id: component2415100
version: 1
top level: BBa_K1321343
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_K1321002
type: Component
persistent identity: component2415101
display id: component2415101
version: 1
top level: BBa_K1321343
access: public
definition: https://synbiohub.org/public/igem/BBa_K1321002/1

BBa_K1321009
type: SequenceAnnotation
persistent identity: annotation2415098
display id: annotation2415098
version: 1
top level: BBa_K1321343
location: https://synbiohub.org/public/igem/BBa_K1321343/annotation2415098/range2415098/1
component: BBa_K1321009

https://synbiohub.org/public/igem/BBa_K1321343/annotation2415098/range2415098/1
type: Range
persistent identity: range2415098
display id: range2415098
version: 1
direction: direction/reverse
top level: BBa_K1321343
start: 1
end: 176
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2415100
display id: annotation2415100
version: 1
top level: BBa_K1321343
location: https://synbiohub.org/public/igem/BBa_K1321343/annotation2415100/range2415100/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_K1321343/annotation2415100/range2415100/1
type: Range
persistent identity: range2415100
display id: range2415100
version: 1
direction: direction/reverse
top level: BBa_K1321343
start: 177
end: 182
orientation: inline

BBa_K1321002
type: SequenceAnnotation
persistent identity: annotation2415101
display id: annotation2415101
version: 1
top level: BBa_K1321343
location: https://synbiohub.org/public/igem/BBa_K1321343/annotation2415101/range2415101/1
component: BBa_K1321002

https://synbiohub.org/public/igem/BBa_K1321343/annotation2415101/range2415101/1
type: Range
persistent identity: range2415101
display id: range2415101
version: 1
direction: direction/reverse
top level: BBa_K1321343
start: 183
end: 482
orientation: inline

BBa_K1399003
type: ComponentDefinition
persistent identity: BBa K1399003
display id: BBa_K1399003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RFP from Discosoma striata (coral) with DAS-ssrA degradation tag
creator: Anna Stikane
created: 2014-09-18T11:00:00Z
modified: 2015-05-08T01:10:16Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1777_
m user id: 0
owner id: 22477
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: Mutant RFP from Discosoma striata (coral) (see part BBa_E1010) with added DAS-ssrA degradation tag (part BBa_M0052). The tag increases RFP turn-over rate, thus providing better temporal resolution of red fluorescence. In the same time, maximal fluorescence amplitudes will be lower as newly formed protein is degraded as soon as it is formed. The tag encodes peptide sequence AANDENYADAS and is recognized by ClpA and ClpX unfoldases and ClpX mediator SspB.[1] ClpA and ClpX then form a proteosome-like complex with ClpP protease and the protein is degraded.[1] The final three residues of the tag determines the strength of interaction with ClpX and thus the final protein degradation rate.[2] The DAS tag is reported to have low affinity to ClpX thus its mediated degradation very much depends on the concentration of SspB (ClpX mediator).[1] However, be aware that exact protein degradation rate is influenced by multiple other factors: ClpXP and ClpAP protease concentrations, protein stability, Km of binding to the protease, temperature [3]. References: [1] Flynn, J. M. et al. Overlapping recognition determinants within the ssrA degradation tag allow modulation of proteolysis. Proc. Natl. Acad. Sci. U. S. A. 98, 10584???9 (2001). [2] Andersen, J. B. et al. New unstable variants of green fluorescent protein for studies of transient gene expression in bacteria. Appl. Environ. Microbiol. 64, 2240???6 (1998). [3] Purcell, O., Grierson, C. S., Bernardo, M. Di & Savery, N. J. Temperature dependence of ssrA-tag mediated protein degradation. J. Biol. Eng. 6, 10 (2012).
mutable notes: The tag was attached to RFP using PCR and MABEL (mutagenesis with blunt-end ligation), thus avoiding introduction of additonal residues and restriction site. Different parts of the tag are recognized by different proteins, for example, the final 3 residues (AAV in this case) are recognised by ClpX, whereas first 4 residues of the tag are required for efficient SspB binding.[1] Thus modifications of these critical residues alter the efficacy with what different proteases bind to it.
mutable provenance: RFP comes from part BBa_E1010, tag sequence was obtained from part BBa_M0052, but same tag was also used in paper by Andersen et al., (1998).[2]
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: DAS-ssrA tag
sequence annotation: RFP
sequence annotation: start
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_K1399003_sequence/1

DAS-ssrA tag
type: SequenceAnnotation
persistent identity: annotation2383905
display id: annotation2383905
version: 1
top level: BBa_K1399003
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1399003/annotation2383905/range2383905/1

https://synbiohub.org/public/igem/BBa_K1399003/annotation2383905/range2383905/1
type: Range
persistent identity: range2383905
display id: range2383905
version: 1
direction: direction/forward
top level: BBa_K1399003
start: 676
end: 708
orientation: inline

RFP
type: SequenceAnnotation
persistent identity: annotation2383904
display id: annotation2383904
version: 1
top level: BBa_K1399003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1399003/annotation2383904/range2383904/1

https://synbiohub.org/public/igem/BBa_K1399003/annotation2383904/range2383904/1
type: Range
persistent identity: range2383904
display id: range2383904
version: 1
direction: direction/forward
top level: BBa_K1399003
start: 4
end: 675
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2383903
display id: annotation2383903
version: 1
top level: BBa_K1399003
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1399003/annotation2383903/range2383903/1

https://synbiohub.org/public/igem/BBa_K1399003/annotation2383903/range2383903/1
type: Range
persistent identity: range2383903
display id: range2383903
version: 1
direction: direction/forward
top level: BBa_K1399003
start: 1
end: 3
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2383906
display id: annotation2383906
version: 1
top level: BBa_K1399003
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399003/annotation2383906/range2383906/1

https://synbiohub.org/public/igem/BBa_K1399003/annotation2383906/range2383906/1
type: Range
persistent identity: range2383906
display id: range2383906
version: 1
direction: direction/forward
top level: BBa_K1399003
start: 709
end: 711
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2383907
display id: annotation2383907
version: 1
top level: BBa_K1399003
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399003/annotation2383907/range2383907/1

https://synbiohub.org/public/igem/BBa_K1399003/annotation2383907/range2383907/1
type: Range
persistent identity: range2383907
display id: range2383907
version: 1
direction: direction/forward
top level: BBa_K1399003
start: 712
end: 714
orientation: inline

BBa_K1758107
type: ComponentDefinition
persistent identity: BBa K1758107
display id: BBa_K1758107
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SYFP2 under T7 promoter + RBS
creator: Team Bielefeld-CeBiTec 2015
created: 2015-08-28T11:00:00Z
modified: 2015-09-18T04:42:23Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2180_
m user id: 27935
owner id: 26563
owning group id: 9
status: status/Planning
bookmark: false
mutable description: SYFP2 is synthesized when T7-polymerase is induced.
mutable notes: ---
mutable provenance: 3A assembly of [http://parts.igem.org/Part:BBa_K525998 BBa_K525998] and [http://parts.igem.org/Part:BBa_K864100 BBa_K864100]
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K525998
component: BBa_K864100
sequence annotation: BBa_K864100
sequence annotation: BBa_K525998
sequence: https://synbiohub.org/public/igem/BBa_K1758107_sequence/1

BBa_K525998
type: Component
persistent identity: component2439947
display id: component2439947
version: 1
top level: BBa_K1758107
access: public
definition: https://synbiohub.org/public/igem/BBa_K525998/1

BBa_K864100
type: Component
persistent identity: component2439949
display id: component2439949
version: 1
top level: BBa_K1758107
access: public
definition: https://synbiohub.org/public/igem/BBa_K864100/1

BBa_K864100
type: SequenceAnnotation
persistent identity: annotation2439949
display id: annotation2439949
version: 1
top level: BBa_K1758107
location: https://synbiohub.org/public/igem/BBa_K1758107/annotation2439949/range2439949/1
component: BBa_K864100

https://synbiohub.org/public/igem/BBa_K1758107/annotation2439949/range2439949/1
type: Range
persistent identity: range2439949
display id: range2439949
version: 1
direction: direction/reverse
top level: BBa_K1758107
start: 39
end: 761
orientation: inline

BBa_K525998
type: SequenceAnnotation
persistent identity: annotation2439947
display id: annotation2439947
version: 1
top level: BBa_K1758107
location: https://synbiohub.org/public/igem/BBa_K1758107/annotation2439947/range2439947/1
component: BBa_K525998

https://synbiohub.org/public/igem/BBa_K1758107/annotation2439947/range2439947/1
type: Range
persistent identity: range2439947
display id: range2439947
version: 1
direction: direction/reverse
top level: BBa_K1758107
start: 1
end: 32
orientation: inline

BBa_K1758314
type: ComponentDefinition
persistent identity: BBa K1758314
display id: BBa_K1758314
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Chromium responsive promoter under T7-promoter with UTR-sfGFP
creator: Team Bielefeld-CeBiTec 2015
created: 2015-09-07T11:00:00Z
modified: 2016-01-06T03:34:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2180_
m user id: 4206
owner id: 26630
owning group id: 9
status: status/Planning
bookmark: false
mutable description: T7 controlled Chromium responsive promoter with 5??untranslated region and sfGFP for detection
mutable notes: ...
mutable provenance: ...
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_I719005
component: BBa_K1758312
sequence annotation: BBa_I719005
sequence annotation: BBa_K1758312
sequence: https://synbiohub.org/public/igem/BBa_K1758314_sequence/1

BBa_I719005
type: Component
persistent identity: component2474963
display id: component2474963
version: 1
top level: BBa_K1758314
access: public
definition: https://synbiohub.org/public/igem/BBa_I719005/1

BBa_K1758312
type: Component
persistent identity: component2474969
display id: component2474969
version: 1
top level: BBa_K1758314
access: public
definition: BBa_K1758312

BBa_I719005
type: SequenceAnnotation
persistent identity: annotation2474963
display id: annotation2474963
version: 1
top level: BBa_K1758314
location: https://synbiohub.org/public/igem/BBa_K1758314/annotation2474963/range2474963/1
component: BBa_I719005

https://synbiohub.org/public/igem/BBa_K1758314/annotation2474963/range2474963/1
type: Range
persistent identity: range2474963
display id: range2474963
version: 1
direction: direction/reverse
top level: BBa_K1758314
start: 1
end: 23
orientation: inline

BBa_K1758312
type: SequenceAnnotation
persistent identity: annotation2474969
display id: annotation2474969
version: 1
top level: BBa_K1758314
location: https://synbiohub.org/public/igem/BBa_K1758314/annotation2474969/range2474969/1
component: BBa_K1758312

https://synbiohub.org/public/igem/BBa_K1758314/annotation2474969/range2474969/1
type: Range
persistent identity: range2474969
display id: range2474969
version: 1
direction: direction/reverse
top level: BBa_K1758314
start: 32
end: 1104
orientation: inline

BBa_K1692006
type: ComponentDefinition
persistent identity: BBa K1692006
display id: BBa_K1692006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: UbiX with T7 Promoter
creator: Daniel Kunin
created: 2015-08-02T11:00:00Z
modified: 2015-08-03T01:00:08Z
discontinued: false
dominant: false
group u list: _2110_
m user id: 26209
owner id: 26209
owning group id: 9
status: status/Planning
bookmark: false
mutable description: UbiX with T7 Promoter
mutable notes: None...
mutable provenance: Escherichia coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K525998
component: BBa_K1692005
sequence annotation: BBa_K1692005
sequence annotation: BBa_K525998
sequence: https://synbiohub.org/public/igem/BBa_K1692006_sequence/1

BBa_K525998
type: Component
persistent identity: component2433981
display id: component2433981
version: 1
top level: BBa_K1692006
access: public
definition: https://synbiohub.org/public/igem/BBa_K525998/1

BBa_K1692005
type: Component
persistent identity: component2433982
display id: component2433982
version: 1
top level: BBa_K1692006
access: public
definition: BBa_K1692005

BBa_K1692005
type: SequenceAnnotation
persistent identity: annotation2433982
display id: annotation2433982
version: 1
top level: BBa_K1692006
location: https://synbiohub.org/public/igem/BBa_K1692006/annotation2433982/range2433982/1
component: BBa_K1692005

https://synbiohub.org/public/igem/BBa_K1692006/annotation2433982/range2433982/1
type: Range
persistent identity: range2433982
display id: range2433982
version: 1
direction: direction/reverse
top level: BBa_K1692006
start: 39
end: 608
orientation: inline

BBa_K525998
type: SequenceAnnotation
persistent identity: annotation2433981
display id: annotation2433981
version: 1
top level: BBa_K1692006
location: https://synbiohub.org/public/igem/BBa_K1692006/annotation2433981/range2433981/1
component: BBa_K525998

https://synbiohub.org/public/igem/BBa_K1692006/annotation2433981/range2433981/1
type: Range
persistent identity: range2433981
display id: range2433981
version: 1
direction: direction/reverse
top level: BBa_K1692006
start: 1
end: 32
orientation: inline

BBa_K1696003
type: ComponentDefinition
persistent identity: BBa K1696003
display id: BBa_K1696003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: L-lactate producing part1
creator: Yue Liu
created: 2015-09-10T11:00:00Z
modified: 2015-09-11T11:49:17Z
discontinued: false
dominant: false
group u list: _2114_
m user id: 16881
owner id: 16881
owning group id: 9
status: status/Planning
bookmark: false
mutable description: We choose a strong promoter+ strong RBS to construct L-Lactate part. The NAD+-dependent L-lactate dehydrogenase gene is from Lactobacillus casei.
mutable notes: Strong promoter+strong RBS
mutable provenance: It's our design based on BBa_K1696001.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: B0014
sequence annotation: ldhE-Lc
sequence annotation: J23100
sequence annotation: B0034
sequence: https://synbiohub.org/public/igem/BBa_K1696003_sequence/1

B0014
type: SequenceAnnotation
persistent identity: annotation2449376
display id: annotation2449376
version: 1
top level: BBa_K1696003
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1696003/annotation2449376/range2449376/1

https://synbiohub.org/public/igem/BBa_K1696003/annotation2449376/range2449376/1
type: Range
persistent identity: range2449376
display id: range2449376
version: 1
direction: direction/forward
top level: BBa_K1696003
start: 1064
end: 1158
orientation: inline

ldhE-Lc
type: SequenceAnnotation
persistent identity: annotation2449375
display id: annotation2449375
version: 1
top level: BBa_K1696003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1696003/annotation2449375/range2449375/1

https://synbiohub.org/public/igem/BBa_K1696003/annotation2449375/range2449375/1
type: Range
persistent identity: range2449375
display id: range2449375
version: 1
direction: direction/forward
top level: BBa_K1696003
start: 69
end: 1049
orientation: inline

J23100
type: SequenceAnnotation
persistent identity: annotation2449370
display id: annotation2449370
version: 1
top level: BBa_K1696003
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1696003/annotation2449370/range2449370/1

https://synbiohub.org/public/igem/BBa_K1696003/annotation2449370/range2449370/1
type: Range
persistent identity: range2449370
display id: range2449370
version: 1
direction: direction/forward
top level: BBa_K1696003
start: 1
end: 35
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2449374
display id: annotation2449374
version: 1
top level: BBa_K1696003
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1696003/annotation2449374/range2449374/1

https://synbiohub.org/public/igem/BBa_K1696003/annotation2449374/range2449374/1
type: Range
persistent identity: range2449374
display id: range2449374
version: 1
direction: direction/forward
top level: BBa_K1696003
start: 51
end: 62
orientation: inline

BBa_K1355002
type: ComponentDefinition
persistent identity: BBa K1355002
display id: BBa_K1355002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Mercury ions detector device
creator: Luna Barroco de Lacerda
created: 2014-10-05T11:00:00Z
modified: 2015-05-08T01:10:02Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1731_
m user id: 0
owner id: 15499
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: We used the biobrick (BBa_E0840) available in the database of the iGEM since 2004 to be connected to our ???essential biobrick??? (BBa_K1355001) and - together - form a system capable of biodetect mercury in bacteria. In the presence of mercury, The MerR protein will bind to it and MerT, MerP and the Green Fluorescent Protein (GFP) protein will be expressed and the biodetection will begin! The MerP and MerT protein are responsible for the transport of mercury from the periplasm to the cytoplasm and the GFP will act as rep??rter to alerting the presence of mercury in the middle and the higher concentration of mercury is, higher will be the GFP expression. And If there is no mercury in the middle, the synthesis of MerT, MerP and GFP protein will be repressed by the MerR repressor protein.
mutable notes: For this construct, we followed these steps: Extraction of plasmid DNA and DNA quantification of bacteria transformed with the Essential Biobrick (BBa_K1355001) and the bacteria transformed with the GFP Biobrick (BBa_E0840); Verifying the electrophoretic profile of the extracted plasmid DNA; Restriction enzyme digestion for Essential Biobrick with SpeI and PstI and for GFP Biobrick with PstI and XbaI; Checking the electrophoretic profile of digested samples; Fragment of interest purification from the gel; Ligation the gel purification of GFP gene and digested Essential Biobrick; Transformation of ligation in DH5-alpha; Plasmid DNA extraction from bacteria transformed; Check the electrophoretic profile to see results of samples linked (no fragments); Digestion of Essential Biobrick + GFP Biobrick with EcoRI and PstI, aiming to analyze the fragment size to be isolated; Checking the electrophoretic profile of the digested sample to obtain results showing that the isolated fragment is the junction of Essential Biobrick +GFP Biobrick ;
mutable provenance: BBa_K1355001, BBa_E0840
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_E0840
component: BBa_K1355001
sequence annotation: BBa_K1355001
sequence annotation: BBa_E0840
sequence: https://synbiohub.org/public/igem/BBa_K1355002_sequence/1

BBa_E0840
type: Component
persistent identity: component2415239
display id: component2415239
version: 1
top level: BBa_K1355002
access: public
definition: https://synbiohub.org/public/igem/BBa_E0840/1

BBa_K1355001
type: Component
persistent identity: component2415228
display id: component2415228
version: 1
top level: BBa_K1355002
access: public
definition: https://synbiohub.org/public/igem/BBa_K1355001/1

BBa_K1355001
type: SequenceAnnotation
persistent identity: annotation2415228
display id: annotation2415228
version: 1
top level: BBa_K1355002
location: https://synbiohub.org/public/igem/BBa_K1355002/annotation2415228/range2415228/1
component: BBa_K1355001

https://synbiohub.org/public/igem/BBa_K1355002/annotation2415228/range2415228/1
type: Range
persistent identity: range2415228
display id: range2415228
version: 1
direction: direction/reverse
top level: BBa_K1355002
start: 1
end: 1189
orientation: inline

BBa_E0840
type: SequenceAnnotation
persistent identity: annotation2415239
display id: annotation2415239
version: 1
top level: BBa_K1355002
location: https://synbiohub.org/public/igem/BBa_K1355002/annotation2415239/range2415239/1
component: BBa_E0840

https://synbiohub.org/public/igem/BBa_K1355002/annotation2415239/range2415239/1
type: Range
persistent identity: range2415239
display id: range2415239
version: 1
direction: direction/reverse
top level: BBa_K1355002
start: 1198
end: 2075
orientation: inline

BBa_K1355003
type: ComponentDefinition
persistent identity: BBa K1355003
display id: BBa_K1355003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Mercury ions accumulator device
creator: Luna Barroco de Lacerda, Maria Clara Astolfi
created: 2014-10-05T11:00:00Z
modified: 2015-05-08T01:10:02Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1731_
m user id: 0
owner id: 15499
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: The Bioaccumulative biobrick is composed of our ???essential biobrick??? (BBa_K1355001) + a Metallothionein (BBa_K346004) available in the database of the iGEM since 2010.In the presence of mercury, The MerR protein will bind to it and MerT, MerP and Metal Binding Protein (MBP) proteins are synthesized and bioaccumulation will start! The MerP and MerT protein are responsible for the transport of mercury from the periplasm to the cytoplasm, leading him to MBP! The MBP binds to the mercury in the cytoplasm, rendering it inactive.
mutable notes: For this construct, we followed these steps: Extraction of plasmid DNA and DNA quantification of bacteria transformed with the Essential Biobrick (BBa_K1355001) and the bacteria transformed with the MBP Biobrick (BBa_K346004); Verifying the electrophoretic profile of the extracted plasmid DNA; Restriction enzyme digestion for Essential Biobrick with SpeI and PstI and for MBP Biobrick with PstI and XbaI; Checking the electrophoretic profile of digested samples; Purification of the Fragment of interest from the gel; Ligation the gel purification of MBP gene and digested Essential Biobrick; Transformation of ligation in DH5-alpha; Plasmid DNA extraction from bacteria transformed; Check the electrophoretic profile to see results of samples linked (no fragments); Digestion of Essential Biobrick + MBP Biobrick with EcoRI and PstI, aiming to analyze the fragment size to be isolated; Checking the electrophoretic profile of the digested sample to obtain results showing that the isolated fragment is the junction of Essential Biobrick +MBP Biobrick ;
mutable provenance: BBa_K1355001; BBa_K346004
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K346004
component: BBa_K1355001
sequence annotation: BBa_K1355001
sequence annotation: BBa_K346004
sequence: https://synbiohub.org/public/igem/BBa_K1355003_sequence/1

BBa_K346004
type: Component
persistent identity: component2415272
display id: component2415272
version: 1
top level: BBa_K1355003
access: public
definition: https://synbiohub.org/public/igem/BBa_K346004/1

BBa_K1355001
type: Component
persistent identity: component2415267
display id: component2415267
version: 1
top level: BBa_K1355003
access: public
definition: https://synbiohub.org/public/igem/BBa_K1355001/1

BBa_K1355001
type: SequenceAnnotation
persistent identity: annotation2415267
display id: annotation2415267
version: 1
top level: BBa_K1355003
location: https://synbiohub.org/public/igem/BBa_K1355003/annotation2415267/range2415267/1
component: BBa_K1355001

https://synbiohub.org/public/igem/BBa_K1355003/annotation2415267/range2415267/1
type: Range
persistent identity: range2415267
display id: range2415267
version: 1
direction: direction/reverse
top level: BBa_K1355003
start: 1
end: 1189
orientation: inline

BBa_K346004
type: SequenceAnnotation
persistent identity: annotation2415272
display id: annotation2415272
version: 1
top level: BBa_K1355003
location: https://synbiohub.org/public/igem/BBa_K1355003/annotation2415272/range2415272/1
component: BBa_K346004

https://synbiohub.org/public/igem/BBa_K1355003/annotation2415272/range2415272/1
type: Range
persistent identity: range2415272
display id: range2415272
version: 1
direction: direction/reverse
top level: BBa_K1355003
start: 1198
end: 1676
orientation: inline

BBa_K1499000
type: ComponentDefinition
persistent identity: BBa K1499000
display id: BBa_K1499000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: wssF gene from wss operon
creator: Jotthe Kannappan
created: 2014-08-26T11:00:00Z
modified: 2015-05-08T01:10:46Z
discontinued: false
dominant: false
group u list: _1879_
m user id: 0
owner id: 22152
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: To do
mutable notes: NA
mutable provenance: The wss operon was extracted from P.fluorescens, an organism that produces cellulose acetate.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Stop Codon: TGA
sequence annotation: Start Codon
sequence annotation: wssF
sequence: https://synbiohub.org/public/igem/BBa_K1499000_sequence/1

Stop Codon: TGA
type: SequenceAnnotation
persistent identity: annotation2381640
display id: annotation2381640
version: 1
top level: BBa_K1499000
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1499000/annotation2381640/range2381640/1

https://synbiohub.org/public/igem/BBa_K1499000/annotation2381640/range2381640/1
type: Range
persistent identity: range2381640
display id: range2381640
version: 1
direction: direction/forward
top level: BBa_K1499000
start: 752
end: 754
orientation: inline

Start Codon
type: SequenceAnnotation
persistent identity: annotation2381639
display id: annotation2381639
version: 1
top level: BBa_K1499000
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1499000/annotation2381639/range2381639/1

https://synbiohub.org/public/igem/BBa_K1499000/annotation2381639/range2381639/1
type: Range
persistent identity: range2381639
display id: range2381639
version: 1
direction: direction/forward
top level: BBa_K1499000
start: 86
end: 88
orientation: inline

wssF
type: SequenceAnnotation
persistent identity: annotation2381638
display id: annotation2381638
version: 1
top level: BBa_K1499000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1499000/annotation2381638/range2381638/1

https://synbiohub.org/public/igem/BBa_K1499000/annotation2381638/range2381638/1
type: Range
persistent identity: range2381638
display id: range2381638
version: 1
direction: direction/forward
top level: BBa_K1499000
start: 86
end: 754
orientation: inline

BBa_K1399001
type: ComponentDefinition
persistent identity: BBa K1399001
display id: BBa_K1399001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RFP from Discosoma striata (coral) with LVA-ssrA degradation tag
creator: Anna Stikane
created: 2014-09-17T11:00:00Z
modified: 2015-05-08T01:10:15Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1777_
m user id: 0
owner id: 22477
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: Mutant RFP from Discosoma striata (coral) (see part BBa_E1010) with added LVA-ssrA degradation tag. The tag increases RFP turn-over rate, thus providing better temporal resolution of red fluorescence. In the same time, maximal fluorescence amplitudes will be lower as newly formed protein is degraded as soon as it is formed. This tag is commonly attached to repressor proteins for use in various gene networks (e.g., oscillators). The tag encodes peptide sequence AANDENYALVA and is recognized by ClpA and ClpX unfoldases and ClpX mediator SspB.[1] ClpA and ClpX then form a proteosome-like complex with ClpP protease and the protein is degraded.[1] The final three residues of the tag determines the strength of interaction with ClpX and thus the final protein degradation rate.[2] The LVA tag is reported to lead to fast protein degradation, degrading GFP with rate -0.018 per minute.[2] However, be aware that exact protein degradation rate depends on multiple factors: ClpXP and ClpAP protease and SspB mediator concentrations; protein stability; Km of binding to the protease; temperature [3]. References: [1] Flynn, J. M. et al. Overlapping recognition determinants within the ssrA degradation tag allow modulation of proteolysis. Proc. Natl. Acad. Sci. U. S. A. 98, 10584???9 (2001). [2] Andersen, J. B. et al. New unstable variants of green fluorescent protein for studies of transient gene expression in bacteria. Appl. Environ. Microbiol. 64, 2240???6 (1998). [3] Purcell, O., Grierson, C. S., Bernardo, M. Di & Savery, N. J. Temperature dependence of ssrA-tag mediated protein degradation. J. Biol. Eng. 6, 10 (2012).
mutable notes: The tag was attached to RFP using PCR and MABEL (mutagenesis with blunt-end ligation), thus avoiding introduction of additonal residues and restriction sites. Different parts of the tag are recognized by different proteins, for example, the final 3 residues (LVA in this case) are recognised by ClpX, whereas first 4 residues of the tag are required for efficient SspB binding.[1] Thus modifications of these critical residues alter the efficacy with what different proteases bind to it.
mutable provenance: RFP comes from part BBa_E1010, tag sequence was obtained from paper by Andersen et al., (1998).[2]
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: cds
sequence annotation: LVA-ssrA tag
sequence annotation: stop
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_K1399001_sequence/1

cds
type: SequenceAnnotation
persistent identity: annotation2383873
display id: annotation2383873
version: 1
top level: BBa_K1399001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1399001/annotation2383873/range2383873/1

https://synbiohub.org/public/igem/BBa_K1399001/annotation2383873/range2383873/1
type: Range
persistent identity: range2383873
display id: range2383873
version: 1
direction: direction/forward
top level: BBa_K1399001
start: 4
end: 675
orientation: inline

LVA-ssrA tag
type: SequenceAnnotation
persistent identity: annotation2383874
display id: annotation2383874
version: 1
top level: BBa_K1399001
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1399001/annotation2383874/range2383874/1

https://synbiohub.org/public/igem/BBa_K1399001/annotation2383874/range2383874/1
type: Range
persistent identity: range2383874
display id: range2383874
version: 1
direction: direction/forward
top level: BBa_K1399001
start: 676
end: 708
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2383876
display id: annotation2383876
version: 1
top level: BBa_K1399001
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399001/annotation2383876/range2383876/1

https://synbiohub.org/public/igem/BBa_K1399001/annotation2383876/range2383876/1
type: Range
persistent identity: range2383876
display id: range2383876
version: 1
direction: direction/forward
top level: BBa_K1399001
start: 712
end: 714
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2383875
display id: annotation2383875
version: 1
top level: BBa_K1399001
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399001/annotation2383875/range2383875/1

https://synbiohub.org/public/igem/BBa_K1399001/annotation2383875/range2383875/1
type: Range
persistent identity: range2383875
display id: range2383875
version: 1
direction: direction/forward
top level: BBa_K1399001
start: 709
end: 711
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2383872
display id: annotation2383872
version: 1
top level: BBa_K1399001
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1399001/annotation2383872/range2383872/1

https://synbiohub.org/public/igem/BBa_K1399001/annotation2383872/range2383872/1
type: Range
persistent identity: range2383872
display id: range2383872
version: 1
direction: direction/forward
top level: BBa_K1399001
start: 1
end: 3
orientation: inline

BBa_K1315007
type: ComponentDefinition
persistent identity: BBa K1315007
display id: BBa_K1315007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: The BCAM0228 Response Regulator from Burkholderia
creator: Dimitrios Michailidis
created: 2014-09-25T11:00:00Z
modified: 2015-05-08T01:09:50Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1690_
m user id: 0
owner id: 21812
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is the coding sequence of the BCAM0228 two-component regulator of the BDSF sensory BCAM0227-BCAM0228 system. This system is found in Burkholderia spp.
mutable notes: The sequence had 1 EcoRI site that was mutated out by site-directed mutagenesis.
mutable provenance: The source of this part is Burkholderia cenocepacia J2315 genomic DNA which was provided by the Robert Ryan lab in the Molecular Microbiology department of the University of Dundee, UK.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: BCAM0228
sequence: https://synbiohub.org/public/igem/BBa_K1315007_sequence/1

BCAM0228
type: SequenceAnnotation
persistent identity: annotation2386928
display id: annotation2386928
version: 1
top level: BBa_K1315007
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1315007/annotation2386928/range2386928/1

https://synbiohub.org/public/igem/BBa_K1315007/annotation2386928/range2386928/1
type: Range
persistent identity: range2386928
display id: range2386928
version: 1
direction: direction/forward
top level: BBa_K1315007
start: 1
end: 888
orientation: inline

BBa_K1699005
type: ComponentDefinition
persistent identity: BBa K1699005
display id: BBa_K1699005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: gRNA for dCas9-VP64 targeting synthetic activation promoter pMLPm under U6 promoter
creator: Emil Ruvinov
created: 2015-08-08T11:00:00Z
modified: 2015-08-09T10:07:27Z
discontinued: false
dominant: false
group u list: _2117_
m user id: 24826
owner id: 24826
owning group id: 9
status: status/Planning
bookmark: false
mutable description: gRNA for dCas9-VP64 targeting synthetic activation promoter pMLPm under U6 promoter
mutable notes: Design
mutable provenance: Source
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: gRNA for pMLPm
sequence annotation: Human U6 promoter
sequence annotation: gRNA scaffold for SpCas9
sequence: https://synbiohub.org/public/igem/BBa_K1699005_sequence/1

gRNA for pMLPm
type: SequenceAnnotation
persistent identity: annotation2434622
display id: annotation2434622
version: 1
top level: BBa_K1699005
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1699005/annotation2434622/range2434622/1

https://synbiohub.org/public/igem/BBa_K1699005/annotation2434622/range2434622/1
type: Range
persistent identity: range2434622
display id: range2434622
version: 1
direction: direction/forward
top level: BBa_K1699005
start: 256
end: 275
orientation: inline

Human U6 promoter
type: SequenceAnnotation
persistent identity: annotation2434621
display id: annotation2434621
version: 1
top level: BBa_K1699005
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1699005/annotation2434621/range2434621/1

https://synbiohub.org/public/igem/BBa_K1699005/annotation2434621/range2434621/1
type: Range
persistent identity: range2434621
display id: range2434621
version: 1
direction: direction/forward
top level: BBa_K1699005
start: 1
end: 249
orientation: inline

gRNA scaffold for SpCas9
type: SequenceAnnotation
persistent identity: annotation2434623
display id: annotation2434623
version: 1
top level: BBa_K1699005
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1699005/annotation2434623/range2434623/1

https://synbiohub.org/public/igem/BBa_K1699005/annotation2434623/range2434623/1
type: Range
persistent identity: range2434623
display id: range2434623
version: 1
direction: direction/forward
top level: BBa_K1699005
start: 276
end: 359
orientation: inline

BBa_K1343014
type: ComponentDefinition
persistent identity: BBa K1343014
display id: BBa_K1343014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Plux-green renilla luciferase
creator: Tal Naor, Ricarina Rabinovitz, Ittai Rubinstein, Yair Maimon
created: 2014-09-26T11:00:00Z
modified: 2015-05-08T01:09:59Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1718_
m user id: 0
owner id: 20512
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: E.coli optimized green renilla luciferase under LuxR-AHL regulated promoter
mutable notes: To assamble this part, and others like it, we used a specialized Gibson Barcode System to allow for a modular construction of gates with various components using the same Gibson reaction conditions, allowing for the assembly of a large number of constructs in parallel to each other
mutable provenance: Placeholder
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: barcode 5
sequence annotation: barcode 2
sequence annotation: green renilla luciferase
sequence annotation: BBa_B0030: Strong RBS
sequence annotation: barcode 3
sequence annotation: BBa_R0062: P lux
sequence annotation: barcode 1
sequence annotation: BBa_B0010:T1 (terminator) from E. coli
sequence annotation: barcode 4
sequence annotation: T7 terminator
sequence annotation: barcode 6
sequence: https://synbiohub.org/public/igem/BBa_K1343014_sequence/1

barcode 5
type: SequenceAnnotation
persistent identity: annotation2428055
display id: annotation2428055
version: 1
top level: BBa_K1343014
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_K1343014/annotation2428055/range2428055/1

https://synbiohub.org/public/igem/BBa_K1343014/annotation2428055/range2428055/1
type: Range
persistent identity: range2428055
display id: range2428055
version: 1
direction: direction/forward
top level: BBa_K1343014
start: 1123
end: 1144
orientation: inline

barcode 2
type: SequenceAnnotation
persistent identity: annotation2428054
display id: annotation2428054
version: 1
top level: BBa_K1343014
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_K1343014/annotation2428054/range2428054/1

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type: Range
persistent identity: range2428054
display id: range2428054
version: 1
direction: direction/forward
top level: BBa_K1343014
start: 1101
end: 1122
orientation: inline

green renilla luciferase
type: SequenceAnnotation
persistent identity: annotation2428052
display id: annotation2428052
version: 1
top level: BBa_K1343014
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1343014/annotation2428052/range2428052/1

https://synbiohub.org/public/igem/BBa_K1343014/annotation2428052/range2428052/1
type: Range
persistent identity: range2428052
display id: range2428052
version: 1
direction: direction/forward
top level: BBa_K1343014
start: 143
end: 1078
orientation: inline

BBa_B0030: Strong RBS
type: SequenceAnnotation
persistent identity: annotation2428051
display id: annotation2428051
version: 1
top level: BBa_K1343014
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1343014/annotation2428051/range2428051/1

https://synbiohub.org/public/igem/BBa_K1343014/annotation2428051/range2428051/1
type: Range
persistent identity: range2428051
display id: range2428051
version: 1
direction: direction/forward
top level: BBa_K1343014
start: 122
end: 136
orientation: inline

barcode 3
type: SequenceAnnotation
persistent identity: annotation2428050
display id: annotation2428050
version: 1
top level: BBa_K1343014
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_K1343014/annotation2428050/range2428050/1

https://synbiohub.org/public/igem/BBa_K1343014/annotation2428050/range2428050/1
type: Range
persistent identity: range2428050
display id: range2428050
version: 1
direction: direction/forward
top level: BBa_K1343014
start: 100
end: 121
orientation: inline

BBa_R0062: P lux
type: SequenceAnnotation
persistent identity: annotation2428047
display id: annotation2428047
version: 1
top level: BBa_K1343014
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1343014/annotation2428047/range2428047/1

https://synbiohub.org/public/igem/BBa_K1343014/annotation2428047/range2428047/1
type: Range
persistent identity: range2428047
display id: range2428047
version: 1
direction: direction/forward
top level: BBa_K1343014
start: 1
end: 55
orientation: inline

barcode 1
type: SequenceAnnotation
persistent identity: annotation2428049
display id: annotation2428049
version: 1
top level: BBa_K1343014
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_K1343014/annotation2428049/range2428049/1

https://synbiohub.org/public/igem/BBa_K1343014/annotation2428049/range2428049/1
type: Range
persistent identity: range2428049
display id: range2428049
version: 1
direction: direction/forward
top level: BBa_K1343014
start: 78
end: 99
orientation: inline

BBa_B0010:T1 (terminator) from E. coli
type: SequenceAnnotation
persistent identity: annotation2428056
display id: annotation2428056
version: 1
top level: BBa_K1343014
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1343014/annotation2428056/range2428056/1

https://synbiohub.org/public/igem/BBa_K1343014/annotation2428056/range2428056/1
type: Range
persistent identity: range2428056
display id: range2428056
version: 1
direction: direction/forward
top level: BBa_K1343014
start: 1145
end: 1224
orientation: inline

barcode 4
type: SequenceAnnotation
persistent identity: annotation2428053
display id: annotation2428053
version: 1
top level: BBa_K1343014
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_K1343014/annotation2428053/range2428053/1

https://synbiohub.org/public/igem/BBa_K1343014/annotation2428053/range2428053/1
type: Range
persistent identity: range2428053
display id: range2428053
version: 1
direction: direction/forward
top level: BBa_K1343014
start: 1079
end: 1100
orientation: inline

T7 terminator
type: SequenceAnnotation
persistent identity: annotation2428057
display id: annotation2428057
version: 1
top level: BBa_K1343014
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1343014/annotation2428057/range2428057/1

https://synbiohub.org/public/igem/BBa_K1343014/annotation2428057/range2428057/1
type: Range
persistent identity: range2428057
display id: range2428057
version: 1
direction: direction/forward
top level: BBa_K1343014
start: 1225
end: 1271
orientation: inline

barcode 6
type: SequenceAnnotation
persistent identity: annotation2428048
display id: annotation2428048
version: 1
top level: BBa_K1343014
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_K1343014/annotation2428048/range2428048/1

https://synbiohub.org/public/igem/BBa_K1343014/annotation2428048/range2428048/1
type: Range
persistent identity: range2428048
display id: range2428048
version: 1
direction: direction/forward
top level: BBa_K1343014
start: 56
end: 77
orientation: inline

BBa_K1321121
type: ComponentDefinition
persistent identity: BBa K1321121
display id: BBa_K1321121
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Smt metallothionein fused to CBDclos
creator: Gabriella Santosa
created: 2014-10-08T11:00:00Z
modified: 2015-06-17T12:22:33Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 4206
owner id: 20961
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: GATC
mutable notes: GATC
mutable provenance: GATC
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0105
component: BBa_K1321011
component: BBa_K1321002
sequence annotation: BBa_B0105
sequence annotation: BBa_K1321011
sequence annotation: BBa_K1321002
sequence: https://synbiohub.org/public/igem/BBa_K1321121_sequence/1

BBa_B0105
type: Component
persistent identity: component2414705
display id: component2414705
version: 1
top level: BBa_K1321121
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_K1321011
type: Component
persistent identity: component2414703
display id: component2414703
version: 1
top level: BBa_K1321121
access: public
definition: https://synbiohub.org/public/igem/BBa_K1321011/1

BBa_K1321002
type: Component
persistent identity: component2414706
display id: component2414706
version: 1
top level: BBa_K1321121
access: public
definition: https://synbiohub.org/public/igem/BBa_K1321002/1

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2414705
display id: annotation2414705
version: 1
top level: BBa_K1321121
location: https://synbiohub.org/public/igem/BBa_K1321121/annotation2414705/range2414705/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_K1321121/annotation2414705/range2414705/1
type: Range
persistent identity: range2414705
display id: range2414705
version: 1
direction: direction/reverse
top level: BBa_K1321121
start: 166
end: 171
orientation: inline

BBa_K1321011
type: SequenceAnnotation
persistent identity: annotation2414703
display id: annotation2414703
version: 1
top level: BBa_K1321121
location: https://synbiohub.org/public/igem/BBa_K1321121/annotation2414703/range2414703/1
component: BBa_K1321011

https://synbiohub.org/public/igem/BBa_K1321121/annotation2414703/range2414703/1
type: Range
persistent identity: range2414703
display id: range2414703
version: 1
direction: direction/reverse
top level: BBa_K1321121
start: 1
end: 165
orientation: inline

BBa_K1321002
type: SequenceAnnotation
persistent identity: annotation2414706
display id: annotation2414706
version: 1
top level: BBa_K1321121
location: https://synbiohub.org/public/igem/BBa_K1321121/annotation2414706/range2414706/1
component: BBa_K1321002

https://synbiohub.org/public/igem/BBa_K1321121/annotation2414706/range2414706/1
type: Range
persistent identity: range2414706
display id: range2414706
version: 1
direction: direction/reverse
top level: BBa_K1321121
start: 172
end: 471
orientation: inline

BBa_K1806001
type: ComponentDefinition
persistent identity: BBa K1806001
display id: BBa_K1806001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Helicobacter pylori catalase
creator: İBRAHİM YASİR ORHAN
created: 2015-09-15T11:00:00Z
modified: 2015-09-17T07:10:18Z
discontinued: false
dominant: false
group u list: _2231_
m user id: 11153
owner id: 11153
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This part is the coding sequence for the Helicobacter pylori catalase, which effectively catalyses the breakdown of hydrogen peroxide. The breakdown of hydrogen peroxide, which is an exothermic process, will be utilized to provide heat to the medium to increase temperature to counteract a shift that decreases the temperature of the host environment.
mutable notes: There are no specific design considerations that were made.
mutable provenance: Helicobacter pylori XZ274 http://www.ncbi.nlm.nih.gov/protein/AFJ81452.1 GenBank: AFJ81452.1
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1806001_sequence/1

BBa_K1402010
type: ComponentDefinition
persistent identity: BBa K1402010
display id: BBa_K1402010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lac Promoter
creator: Chen Xi
created: 2014-09-28T11:00:00Z
modified: 2015-05-08T01:10:16Z
discontinued: false
dominant: false
group u list: _1780_
m user id: 0
owner id: 16730
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part is an inverting regulator sensitive to LacI (BBa_C0010, BBa_C0012, etc.) The T7-lac promoter is a hybrid regulatory region consisting of the T7 promoter of T7 phage next to LacO. The hybrid design allows for strong promotion that can nevertheless be:  repressed by LacI  induced by lac or IPTG, showing a high expression level.  CAP protein will not influence the expression. Strong promoter. Only E.Coli BL21(DE3) can use this part.
mutable notes: no
mutable provenance: E.coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K1402010_sequence/1

BBa_K1592000
type: ComponentDefinition
persistent identity: BBa K1592000
display id: BBa_K1592000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LIP2 prepro(signal peptide)
creator: Shuyan Tang
created: 2015-08-27T11:00:00Z
modified: 2015-09-18T09:23:12Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2009_
m user id: 20267
owner id: 20267
owning group id: 9
status: status/Planning
bookmark: false
mutable description: The pre-region of lipase 2 from Yarrowia lipolytica corresponds to the signal sequence; the dipeptides XA and XP are substrates for diamino-peptidase; the dibasic KR cleavage site is substrate for Xpr6p endoproteinase.
mutable notes: No.
mutable provenance: It have been contributed from our lab.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: LIP2 pro
sequence annotation: LIP2 pre
sequence annotation: 4 XA or XP
sequence annotation: Start codon
sequence annotation: KR cleavage site
sequence: https://synbiohub.org/public/igem/BBa_K1592000_sequence/1

LIP2 pro
type: SequenceAnnotation
persistent identity: annotation2444447
display id: annotation2444447
version: 1
top level: BBa_K1592000
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K1592000/annotation2444447/range2444447/1

https://synbiohub.org/public/igem/BBa_K1592000/annotation2444447/range2444447/1
type: Range
persistent identity: range2444447
display id: range2444447
version: 1
direction: direction/forward
top level: BBa_K1592000
start: 64
end: 93
orientation: inline

LIP2 pre
type: SequenceAnnotation
persistent identity: annotation2444445
display id: annotation2444445
version: 1
top level: BBa_K1592000
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K1592000/annotation2444445/range2444445/1

https://synbiohub.org/public/igem/BBa_K1592000/annotation2444445/range2444445/1
type: Range
persistent identity: range2444445
display id: range2444445
version: 1
direction: direction/forward
top level: BBa_K1592000
start: 1
end: 39
orientation: inline

4 XA or XP
type: SequenceAnnotation
persistent identity: annotation2444446
display id: annotation2444446
version: 1
top level: BBa_K1592000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1592000/annotation2444446/range2444446/1

https://synbiohub.org/public/igem/BBa_K1592000/annotation2444446/range2444446/1
type: Range
persistent identity: range2444446
display id: range2444446
version: 1
direction: direction/forward
top level: BBa_K1592000
start: 40
end: 63
orientation: inline

Start codon
type: SequenceAnnotation
persistent identity: annotation2455904
display id: annotation2455904
version: 1
top level: BBa_K1592000
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1592000/annotation2455904/range2455904/1

https://synbiohub.org/public/igem/BBa_K1592000/annotation2455904/range2455904/1
type: Range
persistent identity: range2455904
display id: range2455904
version: 1
direction: direction/forward
top level: BBa_K1592000
start: 1
end: 3
orientation: inline

KR cleavage site
type: SequenceAnnotation
persistent identity: annotation2444448
display id: annotation2444448
version: 1
top level: BBa_K1592000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1592000/annotation2444448/range2444448/1

https://synbiohub.org/public/igem/BBa_K1592000/annotation2444448/range2444448/1
type: Range
persistent identity: range2444448
display id: range2444448
version: 1
direction: direction/forward
top level: BBa_K1592000
start: 94
end: 99
orientation: inline

BBa_K1789010
type: ComponentDefinition
persistent identity: BBa K1789010
display id: BBa_K1789010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TALE3
creator: Xinyuan Qiu
created: 2015-09-13T11:00:00Z
modified: 2015-09-14T07:28:23Z
discontinued: false
dominant: false
group u list: _2214_
m user id: 21134
owner id: 21134
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This is a engineered TAL effector that can bind with the DNA sequence 5'-CTTTCCACAAATAG-3'
mutable notes: The part sequence should be able to correctly and efficiently bind with corresponding DNA, so we used bioinformatics tools to help us designing this sequence.
mutable provenance: This part is built and standardized by ourselves using GATEWAY technique.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: DNA Binding Domain
sequence annotation: TALE3
sequence: https://synbiohub.org/public/igem/BBa_K1789010_sequence/1

DNA Binding Domain
type: SequenceAnnotation
persistent identity: annotation2454136
display id: annotation2454136
version: 1
top level: BBa_K1789010
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1789010/annotation2454136/range2454136/1

https://synbiohub.org/public/igem/BBa_K1789010/annotation2454136/range2454136/1
type: Range
persistent identity: range2454136
display id: range2454136
version: 1
direction: direction/forward
top level: BBa_K1789010
start: 508
end: 1872
orientation: inline

TALE3
type: SequenceAnnotation
persistent identity: annotation2454135
display id: annotation2454135
version: 1
top level: BBa_K1789010
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1789010/annotation2454135/range2454135/1

https://synbiohub.org/public/igem/BBa_K1789010/annotation2454135/range2454135/1
type: Range
persistent identity: range2454135
display id: range2454135
version: 1
direction: direction/forward
top level: BBa_K1789010
start: 1
end: 2088
orientation: inline

BBa_K1491007
type: ComponentDefinition
persistent identity: BBa K1491007
display id: BBa_K1491007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS-mOrange-His
creator: Danielle Peters
created: 2014-07-14T11:00:00Z
modified: 2015-05-08T01:10:44Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1871_
m user id: 0
owner id: 22521
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: mOrange with 6x His tag(BBa_K1491006) and a strong RBS (BBa_B0034). It can be used as a reporter.
mutable notes: RBS+mOrange+His
mutable provenance: RBS is BBa_B0034 and mOrange is BBa_E2050
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0034
component: BBa_K1491006
sequence annotation: BBa_K1491006
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K1491007_sequence/1

BBa_B0034
type: Component
persistent identity: component2380104
display id: component2380104
version: 1
top level: BBa_K1491007
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1491006
type: Component
persistent identity: component2380107
display id: component2380107
version: 1
top level: BBa_K1491007
access: public
definition: https://synbiohub.org/public/igem/BBa_K1491006/1

BBa_K1491006
type: SequenceAnnotation
persistent identity: annotation2380107
display id: annotation2380107
version: 1
top level: BBa_K1491007
location: https://synbiohub.org/public/igem/BBa_K1491007/annotation2380107/range2380107/1
component: BBa_K1491006

https://synbiohub.org/public/igem/BBa_K1491007/annotation2380107/range2380107/1
type: Range
persistent identity: range2380107
display id: range2380107
version: 1
direction: direction/reverse
top level: BBa_K1491007
start: 19
end: 765
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2380104
display id: annotation2380104
version: 1
top level: BBa_K1491007
location: https://synbiohub.org/public/igem/BBa_K1491007/annotation2380104/range2380104/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1491007/annotation2380104/range2380104/1
type: Range
persistent identity: range2380104
display id: range2380104
version: 1
direction: direction/reverse
top level: BBa_K1491007
start: 1
end: 12
orientation: inline

BBa_K1602050
type: ComponentDefinition
persistent identity: BBa K1602050
display id: BBa_K1602050
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RRlocked
creator: Christian Sator,Stefan Zens, Max Zander, Benedikt Spannenkrebs, Sebastian Jaeger
created: 2015-09-15T11:00:00Z
modified: 2015-09-25T05:13:21Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _2019_
m user id: 23028
owner id: 26220
owning group id: 9
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K1602050_sequence/1

BBa_K1598003
type: ComponentDefinition
persistent identity: BBa K1598003
display id: BBa_K1598003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS-ChAT-6xHis-Terminator
creator: Marta Napiorkowska
created: 2015-08-26T11:00:00Z
modified: 2015-08-28T10:29:27Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2015_
m user id: 27259
owner id: 27180
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This part consists of B0034 RBS, human choline acetyltransferase (CHAT) gene with C-terminal His tag, and B0015 terminator.
mutable notes: - human CHAT gene (UniProt accession no: P28329) was codon-optimized for E.coli and provided with C-terminal 6xHis tag - due to its length, the sequence was synthesized as two gBlock fragments with 30 bp overlap between each other and with 20 bp overlaps with respective PSB1C3 ends - the construct was cloned into PSB1C3 using Gibson Assembly
mutable provenance: DNA synthesis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: Terminator B0015
sequence annotation: RBS B0034
sequence annotation: CHAT
sequence: https://synbiohub.org/public/igem/BBa_K1598003_sequence/1

Terminator B0015
type: SequenceAnnotation
persistent identity: annotation2438654
display id: annotation2438654
version: 1
top level: BBa_K1598003
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1598003/annotation2438654/range2438654/1

https://synbiohub.org/public/igem/BBa_K1598003/annotation2438654/range2438654/1
type: Range
persistent identity: range2438654
display id: range2438654
version: 1
direction: direction/forward
top level: BBa_K1598003
start: 1941
end: 2069
orientation: inline

RBS B0034
type: SequenceAnnotation
persistent identity: annotation2438652
display id: annotation2438652
version: 1
top level: BBa_K1598003
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1598003/annotation2438652/range2438652/1

https://synbiohub.org/public/igem/BBa_K1598003/annotation2438652/range2438652/1
type: Range
persistent identity: range2438652
display id: range2438652
version: 1
direction: direction/forward
top level: BBa_K1598003
start: 1
end: 12
orientation: inline

CHAT
type: SequenceAnnotation
persistent identity: annotation2438653
display id: annotation2438653
version: 1
top level: BBa_K1598003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1598003/annotation2438653/range2438653/1

https://synbiohub.org/public/igem/BBa_K1598003/annotation2438653/range2438653/1
type: Range
persistent identity: range2438653
display id: range2438653
version: 1
direction: direction/forward
top level: BBa_K1598003
start: 19
end: 1932
orientation: inline

GRE3
type: ComponentDefinition
persistent identity: BBa K1602004
display id: BBa_K1602004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Aldose reductase (GRE3) from Saccharomyces cerevisiae S288c
creator: Alexandra Goretzki, Tanja Habeck, Nina Kuschik-Maczollek, Laurin Monnheimer, Steven Pilger, Bianca Reisinger, Daniel Stumpf, Sebastian Barthel
created: 2015-08-31T11:00:00Z
modified: 2015-09-25T01:11:55Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2019_
m user id: 12512
owner id: 25989
owning group id: 9
status: status/Planning
bookmark: false
mutable description: BLANK
mutable notes: BLANK
mutable provenance: Saccharomyces cerevisiae
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1602004_sequence/1

BBa_K1434000
type: ComponentDefinition
persistent identity: BBa K1434000
display id: BBa_K1434000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cAMP receptor protein (CRP) mutant
creator: Greta Jasulaityte
created: 2014-09-14T11:00:00Z
modified: 2015-05-08T01:10:25Z
discontinued: false
dominant: false
group u list: _1812_
m user id: 0
owner id: 20624
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Escherichia coli CRP protein is a cAMP-activated global transcription factor. CRP protein binds cAMP and leads to the activation of transcription of more than one hundred promoters. This protein enhances the possibility of RNA polymerase holoenzyme binding and initiating transcription.
mutable notes: Introduced a mutation from ACTTCA to ATAATT.
mutable provenance: GenBank
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1434000_sequence/1

BBa_K1399000
type: ComponentDefinition
persistent identity: BBa K1399000
display id: BBa_K1399000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RFP from Discosoma striata (coral) with AAV-ssrA degradation tag
creator: Anna Stikane
created: 2014-09-16T11:00:00Z
modified: 2015-05-08T01:10:15Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1777_
m user id: 0
owner id: 22477
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: Mutant RFP from Discosoma striata (coral) (see part E1010) with added AAV-ssrA degradation tag (part I11012). The tag increases RFP turn-over rate, thus providing better temporal resolution of red fluorescence. In the same time, maximal fluorescence amplitudes will be lower as newly formed protein is degraded as soon as it is formed. The tag encodes peptide sequence AANDENYAAAV and is recognized by ClpA and ClpX unfoldases and ClpX mediator SspB.[1] ClpA and ClpX then form a proteosome-like complex with ClpP protease and the protein is degraded.[1] The final three residues of the tag determines the strength of interaction with ClpX and thus the final protein degradation rate.[2] The AAV tag is reported to lead to reasonably fast protein degradation, degrading GFP with rate -0.012 per minute.[2] However, be aware that exact protein degradation rate depends on multiple factors: 1) ClpXP and ClpAP protease and SspB mediator concentrations; 2) Protein stability; 3) Km of binding to the protease; 4) Temperature [3]. [1] Flynn, J. M. et al. Overlapping recognition determinants within the ssrA degradation tag allow modulation of proteolysis. Proc. Natl. Acad. Sci. U. S. A. 98, 10584???9 (2001). [2] Andersen, J. B. et al. New unstable variants of green fluorescent protein for studies of transient gene expression in bacteria. Appl. Environ. Microbiol. 64, 2240???6 (1998). [3]Purcell, O., Grierson, C. S., Bernardo, M. Di & Savery, N. J. Temperature dependence of ssrA-tag mediated protein degradation. J. Biol. Eng. 6, 10 (2012).
mutable notes: The tag was attached to RFP using PCR and MABEL (mutagenesis with blunt-end ligation), thus avoiding introduction of additonal residues and restriction site. Different parts of the tag are recognized by different proteins, for example, the final 3 residues (AAV in this case) are recognised by ClpX, whereas first 4 residues of the tag are required for efficient SspB binding.[1] Thus modifications of these critical residues alter the efficacy with what different proteases bind to it.
mutable provenance: RFP comes from part E1010, tag sequence was obtained from part I11012, but same tag was also usead in paper by Andersen et al., (1998).[2]
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: cds
sequence annotation: stop
sequence annotation: AAV-ssrA tag
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_K1399000_sequence/1

cds
type: SequenceAnnotation
persistent identity: annotation2383855
display id: annotation2383855
version: 1
top level: BBa_K1399000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1399000/annotation2383855/range2383855/1

https://synbiohub.org/public/igem/BBa_K1399000/annotation2383855/range2383855/1
type: Range
persistent identity: range2383855
display id: range2383855
version: 1
direction: direction/forward
top level: BBa_K1399000
start: 4
end: 675
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2383857
display id: annotation2383857
version: 1
top level: BBa_K1399000
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399000/annotation2383857/range2383857/1

https://synbiohub.org/public/igem/BBa_K1399000/annotation2383857/range2383857/1
type: Range
persistent identity: range2383857
display id: range2383857
version: 1
direction: direction/forward
top level: BBa_K1399000
start: 709
end: 711
orientation: inline

AAV-ssrA tag
type: SequenceAnnotation
persistent identity: annotation2383856
display id: annotation2383856
version: 1
top level: BBa_K1399000
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1399000/annotation2383856/range2383856/1

https://synbiohub.org/public/igem/BBa_K1399000/annotation2383856/range2383856/1
type: Range
persistent identity: range2383856
display id: range2383856
version: 1
direction: direction/forward
top level: BBa_K1399000
start: 676
end: 708
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2383854
display id: annotation2383854
version: 1
top level: BBa_K1399000
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1399000/annotation2383854/range2383854/1

https://synbiohub.org/public/igem/BBa_K1399000/annotation2383854/range2383854/1
type: Range
persistent identity: range2383854
display id: range2383854
version: 1
direction: direction/forward
top level: BBa_K1399000
start: 1
end: 3
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2383858
display id: annotation2383858
version: 1
top level: BBa_K1399000
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399000/annotation2383858/range2383858/1

https://synbiohub.org/public/igem/BBa_K1399000/annotation2383858/range2383858/1
type: Range
persistent identity: range2383858
display id: range2383858
version: 1
direction: direction/forward
top level: BBa_K1399000
start: 712
end: 714
orientation: inline

His-Scaffo
type: ComponentDefinition
persistent identity: BBa K1602027
display id: BBa_K1602027
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Protein scaffold with N-terminal HisTag
creator: Sebastian Barthel, Niklas Hummel, Jasmin Weber, Michelle Zoeller, Marcus Geissler, Stefan Bohn, Simon Linder
created: 2015-09-15T11:00:00Z
modified: 2015-09-16T06:19:29Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2019_
m user id: 22022
owner id: 22022
owning group id: 9
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1602027_sequence/1

BBa_K1321347
type: ComponentDefinition
persistent identity: BBa K1321347
display id: BBa_K1321347
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: dCBD with N-terminal linker in RFC 25
creator: Chris N Micklem
created: 2014-10-07T11:00:00Z
modified: 2015-05-08T01:09:53Z
discontinued: true
dominant: false
experience: experience/None
group u list: _1696_
m user id: 0
owner id: 20830
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: asdf
mutable notes: asdf
mutable provenance: asdf
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: dCBD
sequence: https://synbiohub.org/public/igem/BBa_K1321347_sequence/1

dCBD
type: SequenceAnnotation
persistent identity: annotation2414642
display id: annotation2414642
version: 1
top level: BBa_K1321347
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1321347/annotation2414642/range2414642/1

https://synbiohub.org/public/igem/BBa_K1321347/annotation2414642/range2414642/1
type: Range
persistent identity: range2414642
display id: range2414642
version: 1
direction: direction/forward
top level: BBa_K1321347
start: 1
end: 366
orientation: inline

BBa_K1640018
type: ComponentDefinition
persistent identity: BBa K1640018
display id: BBa_K1640018
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CHlM
creator: Meghan Cook
created: 2015-09-09T11:00:00Z
modified: 2016-10-20T07:15:09Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2057_
m user id: 7708
owner id: 28565
owning group id: 9
status: status/Planning
bookmark: true
mutable description: n/a
mutable notes: n/a
mutable provenance: n/a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: ChlM
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_K1640018_sequence/1

ChlM
type: SequenceAnnotation
persistent identity: annotation2474919
display id: annotation2474919
version: 1
top level: BBa_K1640018
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1640018/annotation2474919/range2474919/1

https://synbiohub.org/public/igem/BBa_K1640018/annotation2474919/range2474919/1
type: Range
persistent identity: range2474919
display id: range2474919
version: 1
direction: direction/forward
top level: BBa_K1640018
start: 20
end: 862
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2474918
display id: annotation2474918
version: 1
top level: BBa_K1640018
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1640018/annotation2474918/range2474918/1

https://synbiohub.org/public/igem/BBa_K1640018/annotation2474918/range2474918/1
type: Range
persistent identity: range2474918
display id: range2474918
version: 1
direction: direction/forward
top level: BBa_K1640018
start: 1
end: 11
orientation: inline

BBa_K1399002
type: ComponentDefinition
persistent identity: BBa K1399002
display id: BBa_K1399002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RFP from Discosoma striata (coral) with LAA-ssrA degradation tag (wt)
creator: Anna Stikane
created: 2014-09-18T11:00:00Z
modified: 2015-05-08T01:10:15Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1777_
m user id: 0
owner id: 22477
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: Mutant RFP from Discosoma striata (coral) (see part BBa_E1010) with added LAA-ssrA degradation tag (part BBa_M0050). The tag increases RFP turn-over rate, thus providing better temporal resolution of red fluorescence. In the same time, maximal fluorescence amplitudes will be lower as newly formed protein is degraded as soon as it is formed. The tag encodes peptide sequence AANDENYALAA and is recognized by ClpA and ClpX unfoldases and ClpX mediator SspB.[1] ClpA and ClpX then form a proteosome-like complex with ClpP protease and the protein is degraded.[1] The final three residues of the tag determines the strength of interaction with ClpX and thus the final protein degradation rate.[2] The LAA tag is E. coli wild type ssrA tag and is reported to lead to very fast protein degradation, degrading GFP with rate -0.018 per minute.[2] However, be aware that exact protein degradation rate depends on multiple factors: ClpXP and ClpAP protease and SspB mediator concentrations; Protein stability; Km of binding to the protease; Temperature [3]. Mutant RFP from Discosoma striata (coral) (see part BBa_E1010) with added LAA-ssrA degradation tag (part BBa_M0050). The tag increases RFP turn-over rate, thus providing better temporal resolution of red fluorescence. In the same time, maximal fluorescence amplitudes will be lower as newly formed protein is degraded as soon as it is formed. The tag encodes peptide sequence AANDENYALAA and is recognized by ClpA and ClpX unfoldases and ClpX mediator SspB.[1] ClpA and ClpX then form a proteosome-like complex with ClpP protease and the protein is degraded.[1] The final three residues of the tag determines the strength of interaction with ClpX and thus the final protein degradation rate.[2] The LAA tag is E. coli wild type ssrA tag and is reported to lead to very fast protein degradation, degrading GFP with rate -0.018 per minute.[2] However, be aware that exact protein degradation rate depends on multiple factors: ClpXP and ClpAP protease a…
mutable notes: The tag was attached to RFP using PCR and MABEL (mutagenesis with blunt-end ligation), thus avoiding introduction of additonal residues and restriction site. Different parts of the tag are recognized by different proteins, for example, the final 3 residues (AAV in this case) are recognised by ClpX, whereas first 4 residues of the tag are required for efficient SspB binding.[1] Thus modifications of these critical residues alter the efficacy with what different proteases bind to it.
mutable provenance: RFP comes from part BBa_E1010, tag sequence was obtained from part BBa_M0050, but same tag was also used in paper by Andersen et al., (1998).[2]
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: tag
sequence annotation: stop
sequence annotation: start
sequence annotation: RFP
sequence: https://synbiohub.org/public/igem/BBa_K1399002_sequence/1

tag
type: SequenceAnnotation
persistent identity: annotation2383900
display id: annotation2383900
version: 1
top level: BBa_K1399002
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1399002/annotation2383900/range2383900/1

https://synbiohub.org/public/igem/BBa_K1399002/annotation2383900/range2383900/1
type: Range
persistent identity: range2383900
display id: range2383900
version: 1
direction: direction/forward
top level: BBa_K1399002
start: 676
end: 708
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2383901
display id: annotation2383901
version: 1
top level: BBa_K1399002
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399002/annotation2383901/range2383901/1

https://synbiohub.org/public/igem/BBa_K1399002/annotation2383901/range2383901/1
type: Range
persistent identity: range2383901
display id: range2383901
version: 1
direction: direction/forward
top level: BBa_K1399002
start: 709
end: 711
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2383898
display id: annotation2383898
version: 1
top level: BBa_K1399002
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1399002/annotation2383898/range2383898/1

https://synbiohub.org/public/igem/BBa_K1399002/annotation2383898/range2383898/1
type: Range
persistent identity: range2383898
display id: range2383898
version: 1
direction: direction/forward
top level: BBa_K1399002
start: 1
end: 3
orientation: inline

RFP
type: SequenceAnnotation
persistent identity: annotation2383899
display id: annotation2383899
version: 1
top level: BBa_K1399002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1399002/annotation2383899/range2383899/1

https://synbiohub.org/public/igem/BBa_K1399002/annotation2383899/range2383899/1
type: Range
persistent identity: range2383899
display id: range2383899
version: 1
direction: direction/forward
top level: BBa_K1399002
start: 4
end: 675
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2383902
display id: annotation2383902
version: 1
top level: BBa_K1399002
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399002/annotation2383902/range2383902/1

https://synbiohub.org/public/igem/BBa_K1399002/annotation2383902/range2383902/1
type: Range
persistent identity: range2383902
display id: range2383902
version: 1
direction: direction/forward
top level: BBa_K1399002
start: 712
end: 714
orientation: inline

BBa_K1349000
type: ComponentDefinition
persistent identity: BBa K1349000
display id: BBa_K1349000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: serA(1-->1008bp)
creator: Alexia Satouf, Clara Bouyx, Aimeric Agaoua, Lambert Antoni, Vincent Castel
created: 2014-10-05T11:00:00Z
modified: 2015-05-08T01:10:01Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1724_
m user id: 0
owner id: 6328
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This construct encodes a truncated version of E. coli W3110 SerA ( D-3-phosphoglycerate dehydrogenase), missing the last 75 aa of the full sequence, and without stop codon. SerA is requiered for serine biosynthesis. Following the work of Peters-Wendisch et al. 2005, this mutated version of the enzyme is expected to be no-longer inhibited by Serine, and to allow the production of a high serine concentration.
mutable notes: The part was obtained by PCR from E.coli strain W3110 and SLIC assembly. The construction removed the EcoR1 restriction sites in the gene by the silent mutations of a GAA codon to a GAG codon.
mutable provenance: Amplified by PCR from E.coli W3110 genomic DNA.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1349000_sequence/1

BBa_K1349007
type: ComponentDefinition
persistent identity: BBa K1349007
display id: BBa_K1349007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cusR-lza
creator: Alexia Satouf, Clara Bouyx, Aimeric Agaoua, Lambert Antoni, Vincent Castel
created: 2014-10-05T11:00:00Z
modified: 2015-05-08T01:10:01Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1724_
m user id: 0
owner id: 6328
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Response regulator of the cusCFBA-cusRS divergent operons fused with a leucine zipper.
mutable notes: xxx
mutable provenance: Amplified from pWW2021,Whitaker et al. PNAS 2012
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1349007_sequence/1

BBa_K1399006
type: ComponentDefinition
persistent identity: BBa K1399006
display id: BBa_K1399006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP (mut3b) with LAA-ssrA degradation tag
creator: Anna Stikane
created: 2014-09-18T11:00:00Z
modified: 2015-05-08T01:10:16Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1777_
m user id: 0
owner id: 22477
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: GFP (mut3b) (see part BBa_E0040) with added LAA-ssrA degradation tag (see part BBa_M0050). The tag increases GFP turn-over rate, thus providing better temporal resolution of green fluorescence. In the same time, maximal fluorescence amplitudes will be lower as newly formed protein is degraded as soon as it is formed. The tag encodes E.coli wild type ssrA peptide sequence AANDENYALAA and is recognized by ClpA and ClpX unfoldases and ClpX mediator SspB.[1] ClpA and ClpX then form a proteosome-like complex with ClpP protease and the protein is degraded.[1] The final three residues of the tag determines the strength of interaction with ClpX and thus the final protein degradation rate.[2] The LAA tag is reported to lead to fast protein degradation, degrading GFP with rate -0.018 per minute.[2] However, be aware that exact protein degradation rate depends on multiple factors: ClpXP and ClpAP protease and SspB mediator concentrations, protein stability, Km of binding to the protease, temperature [3]. ===References=== [1] Flynn, J. M. et al. Overlapping recognition determinants within the ssrA degradation tag allow modulation of proteolysis. Proc. Natl. Acad. Sci. U. S. A. 98, 10584???9 (2001). [2] Andersen, J. B. et al. New unstable variants of green fluorescent protein for studies of transient gene expression in bacteria. Appl. Environ. Microbiol. 64, 2240???6 (1998). [3] Purcell, O., Grierson, C. S., Bernardo, M. Di & Savery, N. J. Temperature dependence of ssrA-tag mediated protein degradation. J. Biol. Eng. 6, 10 (2012).
mutable notes: The tag was attached to GFP using PCR and MABEL (mutagenesis with blunt-end ligation), thus avoiding introduction of additional residues and restriction site. Different parts of the tag are recognized by different proteins, for example, the final 3 residues (LAA in this case) are recognized by ClpX, whereas first 4 residues of the tag are required for efficient SspB binding.[1] Thus modifications of these critical residues alter the efficacy with what different proteases bind to it.
mutable provenance: GFP comes from part BBa_E1010, the tag sequence was obtained from part BBa_M0050.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: GFP (mut3b)
sequence annotation: start
sequence annotation: stop
sequence annotation: LAA-ssrA
sequence: https://synbiohub.org/public/igem/BBa_K1399006_sequence/1

GFP (mut3b)
type: SequenceAnnotation
persistent identity: annotation2383919
display id: annotation2383919
version: 1
top level: BBa_K1399006
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1399006/annotation2383919/range2383919/1

https://synbiohub.org/public/igem/BBa_K1399006/annotation2383919/range2383919/1
type: Range
persistent identity: range2383919
display id: range2383919
version: 1
direction: direction/forward
top level: BBa_K1399006
start: 4
end: 714
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2383918
display id: annotation2383918
version: 1
top level: BBa_K1399006
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1399006/annotation2383918/range2383918/1

https://synbiohub.org/public/igem/BBa_K1399006/annotation2383918/range2383918/1
type: Range
persistent identity: range2383918
display id: range2383918
version: 1
direction: direction/forward
top level: BBa_K1399006
start: 1
end: 3
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2383922
display id: annotation2383922
version: 1
top level: BBa_K1399006
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399006/annotation2383922/range2383922/1

https://synbiohub.org/public/igem/BBa_K1399006/annotation2383922/range2383922/1
type: Range
persistent identity: range2383922
display id: range2383922
version: 1
direction: direction/forward
top level: BBa_K1399006
start: 751
end: 753
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2383921
display id: annotation2383921
version: 1
top level: BBa_K1399006
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399006/annotation2383921/range2383921/1

https://synbiohub.org/public/igem/BBa_K1399006/annotation2383921/range2383921/1
type: Range
persistent identity: range2383921
display id: range2383921
version: 1
direction: direction/forward
top level: BBa_K1399006
start: 748
end: 750
orientation: inline

LAA-ssrA
type: SequenceAnnotation
persistent identity: annotation2383920
display id: annotation2383920
version: 1
top level: BBa_K1399006
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1399006/annotation2383920/range2383920/1

https://synbiohub.org/public/igem/BBa_K1399006/annotation2383920/range2383920/1
type: Range
persistent identity: range2383920
display id: range2383920
version: 1
direction: direction/forward
top level: BBa_K1399006
start: 715
end: 747
orientation: inline

BBa_K1763010
type: ComponentDefinition
persistent identity: BBa K1763010
display id: BBa_K1763010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Major Spidroin Protein 1 (MaSp1) with Sticky Ends AB
creator: Vinson Lam
created: 2015-07-26T11:00:00Z
modified: 2015-09-16T10:43:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2185_
m user id: 27136
owner id: 27136
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This part contains the core sequence of MaSp1 that has been assembled with a 5'-AGTT-3' overhang on the 5' end of the sequence and 3'-ACAG-5' overhang on the 3' end of the sequence. This part was designed for use with ICA, along with parts BBa_K1763003 and BBa_K1763004 as an efficient method to assemble multiple MaSp sequences together. This setup is to show that this particular set of sticky ends is suitable for ICA. Before using in ICA, this part should be digested with BsaI, a type IIs restriction enzyme, which cuts DNA outside of its recognition site.
mutable notes: The gene sequence for MaSp2 is particularly GC-rich, which may complicate PCR.
mutable provenance: This part is derived from the MaSp2 gene found in the spider N. Clavipes.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: BsaI recognition site (reverse)
sequence annotation: MaSp1 Core
sequence annotation: BsaI recognition site (forward)
sequence annotation: A sticky end
sequence annotation: B sticky end
sequence: https://synbiohub.org/public/igem/BBa_K1763010_sequence/1

BsaI recognition site (reverse)
type: SequenceAnnotation
persistent identity: annotation2433553
display id: annotation2433553
version: 1
top level: BBa_K1763010
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1763010/annotation2433553/range2433553/1

https://synbiohub.org/public/igem/BBa_K1763010/annotation2433553/range2433553/1
type: Range
persistent identity: range2433553
display id: range2433553
version: 1
direction: direction/forward
top level: BBa_K1763010
start: 114
end: 119
orientation: inline

MaSp1 Core
type: SequenceAnnotation
persistent identity: annotation2473356
display id: annotation2473356
version: 1
top level: BBa_K1763010
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763010/annotation2473356/range2473356/1

https://synbiohub.org/public/igem/BBa_K1763010/annotation2473356/range2473356/1
type: Range
persistent identity: range2473356
display id: range2473356
version: 1
direction: direction/forward
top level: BBa_K1763010
start: 10
end: 108
orientation: inline

BsaI recognition site (forward)
type: SequenceAnnotation
persistent identity: annotation2433552
display id: annotation2433552
version: 1
top level: BBa_K1763010
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1763010/annotation2433552/range2433552/1

https://synbiohub.org/public/igem/BBa_K1763010/annotation2433552/range2433552/1
type: Range
persistent identity: range2433552
display id: range2433552
version: 1
direction: direction/forward
top level: BBa_K1763010
start: 1
end: 5
orientation: inline

A sticky end
type: SequenceAnnotation
persistent identity: annotation2463772
display id: annotation2463772
version: 1
top level: BBa_K1763010
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1763010/annotation2463772/range2463772/1

https://synbiohub.org/public/igem/BBa_K1763010/annotation2463772/range2463772/1
type: Range
persistent identity: range2463772
display id: range2463772
version: 1
direction: direction/forward
top level: BBa_K1763010
start: 7
end: 10
orientation: inline

B sticky end
type: SequenceAnnotation
persistent identity: annotation2463773
display id: annotation2463773
version: 1
top level: BBa_K1763010
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1763010/annotation2463773/range2463773/1

https://synbiohub.org/public/igem/BBa_K1763010/annotation2463773/range2463773/1
type: Range
persistent identity: range2463773
display id: range2463773
version: 1
direction: direction/forward
top level: BBa_K1763010
start: 109
end: 112
orientation: inline

RBS-GFP_Si
type: ComponentDefinition
persistent identity: BBa K1602025
display id: BBa_K1602025
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: HisTag-GFP_Si4-Tag fusion protein with RBS BBa_B0034
creator: Jasmin Weber, Michelle Zoeller, Marcus Geissler, Stefan Bohn, Simon Linder, Sebastian Barthel
created: 2015-09-15T11:00:00Z
modified: 2015-09-16T05:56:03Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2019_
m user id: 22022
owner id: 22022
owning group id: 9
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0034
component: BBa_K1602024
sequence annotation: BBa_K1602024
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K1602025_sequence/1

BBa_B0034
type: Component
persistent identity: component2462563
display id: component2462563
version: 1
top level: RBS-GFP_Si
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1602024
type: Component
persistent identity: component2462564
display id: component2462564
version: 1
top level: RBS-GFP_Si
access: public
definition: https://synbiohub.org/public/igem/BBa_K1602024/1

BBa_K1602024
type: SequenceAnnotation
persistent identity: annotation2462564
display id: annotation2462564
version: 1
top level: RBS-GFP_Si
location: https://synbiohub.org/public/igem/BBa_K1602025/annotation2462564/range2462564/1
component: BBa_K1602024

https://synbiohub.org/public/igem/BBa_K1602025/annotation2462564/range2462564/1
type: Range
persistent identity: range2462564
display id: range2462564
version: 1
direction: direction/reverse
top level: RBS-GFP_Si
start: 19
end: 825
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2462563
display id: annotation2462563
version: 1
top level: RBS-GFP_Si
location: https://synbiohub.org/public/igem/BBa_K1602025/annotation2462563/range2462563/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1602025/annotation2462563/range2462563/1
type: Range
persistent identity: range2462563
display id: range2462563
version: 1
direction: direction/reverse
top level: RBS-GFP_Si
start: 1
end: 12
orientation: inline

RBS-Scaffo
type: ComponentDefinition
persistent identity: BBa K1602028
display id: BBa_K1602028
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Protein scaffold with N-terminal HisTag and RBS BBa_B0034
creator: Sebastian Barthel, Niklas Hummel, Jasmin Weber, Michelle Zoeller, Marcus Geissler, Stefan Bohn, Simon Linder
created: 2015-09-15T11:00:00Z
modified: 2015-09-16T06:25:50Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2019_
m user id: 22022
owner id: 22022
owning group id: 9
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1602027
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_K1602027
sequence: https://synbiohub.org/public/igem/BBa_K1602028_sequence/1

BBa_K1602027
type: Component
persistent identity: component2462703
display id: component2462703
version: 1
top level: RBS-Scaffo
access: public
definition: His-Scaffo

BBa_B0034
type: Component
persistent identity: component2462702
display id: component2462702
version: 1
top level: RBS-Scaffo
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2462702
display id: annotation2462702
version: 1
top level: RBS-Scaffo
location: https://synbiohub.org/public/igem/BBa_K1602028/annotation2462702/range2462702/1
component: BBa_B0034

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type: Range
persistent identity: range2462702
display id: range2462702
version: 1
direction: direction/reverse
top level: RBS-Scaffo
start: 1
end: 12
orientation: inline

BBa_K1602027
type: SequenceAnnotation
persistent identity: annotation2462703
display id: annotation2462703
version: 1
top level: RBS-Scaffo
location: https://synbiohub.org/public/igem/BBa_K1602028/annotation2462703/range2462703/1
component: BBa_K1602027

https://synbiohub.org/public/igem/BBa_K1602028/annotation2462703/range2462703/1
type: Range
persistent identity: range2462703
display id: range2462703
version: 1
direction: direction/reverse
top level: RBS-Scaffo
start: 19
end: 900
orientation: inline

BBa_K1763440
type: ComponentDefinition
persistent identity: BBa K1763440
display id: BBa_K1763440
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: MaSp1/2[1:1]-12(1C3)
creator: Vinson Lam
created: 2015-09-14T11:00:00Z
modified: 2015-09-17T12:02:30Z
discontinued: false
dominant: false
group u list: _2185_
m user id: 27136
owner id: 27136
owning group id: 9
status: status/Planning
bookmark: false
mutable description: MaSp1 and MaSp2 12-mer block co-polymer in pSB1C3. No regulatory elements present. Ratio of 1 MaSp1 to 1 MaSp2.
mutable notes: no.
mutable provenance: N. clavipes
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: M2-AB
sequence annotation: M1-AB
sequence annotation: M1-BC
sequence annotation: 8-his tag
sequence annotation: M1-CA
sequence annotation: M2-BC
sequence annotation: M2-CA
sequence: https://synbiohub.org/public/igem/BBa_K1763440_sequence/1

M2-AB
type: SequenceAnnotation
persistent identity: annotation2464104
display id: annotation2464104
version: 1
top level: BBa_K1763440
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763440/annotation2464104/range2464104/1

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type: Range
persistent identity: range2464104
display id: range2464104
version: 1
direction: direction/forward
top level: BBa_K1763440
start: 954
end: 1055
orientation: inline

M1-AB
type: SequenceAnnotation
persistent identity: annotation2464111
display id: annotation2464111
version: 1
top level: BBa_K1763440
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763440/annotation2464111/range2464111/1

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type: Range
persistent identity: range2464111
display id: range2464111
version: 1
direction: direction/forward
top level: BBa_K1763440
start: 36
end: 137
orientation: inline

M1-BC
type: SequenceAnnotation
persistent identity: annotation2464105
display id: annotation2464105
version: 1
top level: BBa_K1763440
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763440/annotation2464105/range2464105/1

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type: Range
persistent identity: range2464105
display id: range2464105
version: 1
direction: direction/forward
top level: BBa_K1763440
start: 138
end: 239
orientation: inline

8-his tag
type: SequenceAnnotation
persistent identity: annotation2464099
display id: annotation2464099
version: 1
top level: BBa_K1763440
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1763440/annotation2464099/range2464099/1

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type: Range
persistent identity: range2464099
display id: range2464099
version: 1
direction: direction/forward
top level: BBa_K1763440
start: 4
end: 27
orientation: inline

M1-CA
type: SequenceAnnotation
persistent identity: annotation2464110
display id: annotation2464110
version: 1
top level: BBa_K1763440
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763440/annotation2464110/range2464110/1

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type: Range
persistent identity: range2464110
display id: range2464110
version: 1
direction: direction/forward
top level: BBa_K1763440
start: 546
end: 647
orientation: inline

M2-BC
type: SequenceAnnotation
persistent identity: annotation2464106
display id: annotation2464106
version: 1
top level: BBa_K1763440
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763440/annotation2464106/range2464106/1

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type: Range
persistent identity: range2464106
display id: range2464106
version: 1
direction: direction/forward
top level: BBa_K1763440
start: 750
end: 851
orientation: inline

M2-AB
type: SequenceAnnotation
persistent identity: annotation2464107
display id: annotation2464107
version: 1
top level: BBa_K1763440
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763440/annotation2464107/range2464107/1

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type: Range
persistent identity: range2464107
display id: range2464107
version: 1
direction: direction/forward
top level: BBa_K1763440
start: 648
end: 749
orientation: inline

M1-BC
type: SequenceAnnotation
persistent identity: annotation2464100
display id: annotation2464100
version: 1
top level: BBa_K1763440
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763440/annotation2464100/range2464100/1

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type: Range
persistent identity: range2464100
display id: range2464100
version: 1
direction: direction/forward
top level: BBa_K1763440
start: 444
end: 545
orientation: inline

M2-BC
type: SequenceAnnotation
persistent identity: annotation2464103
display id: annotation2464103
version: 1
top level: BBa_K1763440
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763440/annotation2464103/range2464103/1

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type: Range
persistent identity: range2464103
display id: range2464103
version: 1
direction: direction/forward
top level: BBa_K1763440
start: 1056
end: 1157
orientation: inline

M2-CA
type: SequenceAnnotation
persistent identity: annotation2464101
display id: annotation2464101
version: 1
top level: BBa_K1763440
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763440/annotation2464101/range2464101/1

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type: Range
persistent identity: range2464101
display id: range2464101
version: 1
direction: direction/forward
top level: BBa_K1763440
start: 852
end: 953
orientation: inline

M1-AB
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persistent identity: annotation2464108
display id: annotation2464108
version: 1
top level: BBa_K1763440
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763440/annotation2464108/range2464108/1

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type: Range
persistent identity: range2464108
display id: range2464108
version: 1
direction: direction/forward
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start: 342
end: 443
orientation: inline

M2-CA
type: SequenceAnnotation
persistent identity: annotation2464102
display id: annotation2464102
version: 1
top level: BBa_K1763440
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763440/annotation2464102/range2464102/1

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type: Range
persistent identity: range2464102
display id: range2464102
version: 1
direction: direction/forward
top level: BBa_K1763440
start: 1158
end: 1259
orientation: inline

M1-CA
type: SequenceAnnotation
persistent identity: annotation2464109
display id: annotation2464109
version: 1
top level: BBa_K1763440
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763440/annotation2464109/range2464109/1

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type: Range
persistent identity: range2464109
display id: range2464109
version: 1
direction: direction/forward
top level: BBa_K1763440
start: 240
end: 341
orientation: inline

BBa_K1689002
type: ComponentDefinition
persistent identity: BBa K1689002
display id: BBa_K1689002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Coding sequence of Cluc-STV
creator: ZHANG Yihao
created: 2015-09-15T11:00:00Z
modified: 2015-09-27T01:19:10Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2107_
m user id: 26531
owner id: 26464
owning group id: 9
status: status/Planning
bookmark: false
mutable description: including firefly luciferase C terminal 398 amino acid domain, 2??linker, and streptavidin.
mutable notes: firefly luciferase C terminal 398 amino acid domain and streptavidin are linked by 2??linker
mutable provenance: E.coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: linker
sequence annotation: streptavidin
sequence annotation: CFluc398
sequence annotation: random spacer
sequence annotation: stop codon
sequence annotation: start codon
sequence: https://synbiohub.org/public/igem/BBa_K1689002_sequence/1

linker
type: SequenceAnnotation
persistent identity: annotation2469946
display id: annotation2469946
version: 1
top level: BBa_K1689002
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1689002/annotation2469946/range2469946/1

https://synbiohub.org/public/igem/BBa_K1689002/annotation2469946/range2469946/1
type: Range
persistent identity: range2469946
display id: range2469946
version: 1
direction: direction/forward
top level: BBa_K1689002
start: 496
end: 525
orientation: inline

streptavidin
type: SequenceAnnotation
persistent identity: annotation2469948
display id: annotation2469948
version: 1
top level: BBa_K1689002
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1689002/annotation2469948/range2469948/1

https://synbiohub.org/public/igem/BBa_K1689002/annotation2469948/range2469948/1
type: Range
persistent identity: range2469948
display id: range2469948
version: 1
direction: direction/forward
top level: BBa_K1689002
start: 526
end: 879
orientation: inline

CFluc398
type: SequenceAnnotation
persistent identity: annotation2469944
display id: annotation2469944
version: 1
top level: BBa_K1689002
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1689002/annotation2469944/range2469944/1

https://synbiohub.org/public/igem/BBa_K1689002/annotation2469944/range2469944/1
type: Range
persistent identity: range2469944
display id: range2469944
version: 1
direction: direction/forward
top level: BBa_K1689002
start: 40
end: 495
orientation: inline

random spacer
type: SequenceAnnotation
persistent identity: annotation2477764
display id: annotation2477764
version: 1
top level: BBa_K1689002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1689002/annotation2477764/range2477764/1

https://synbiohub.org/public/igem/BBa_K1689002/annotation2477764/range2477764/1
type: Range
persistent identity: range2477764
display id: range2477764
version: 1
direction: direction/forward
top level: BBa_K1689002
start: 883
end: 937
orientation: inline

random spacer
type: SequenceAnnotation
persistent identity: annotation2477763
display id: annotation2477763
version: 1
top level: BBa_K1689002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1689002/annotation2477763/range2477763/1

https://synbiohub.org/public/igem/BBa_K1689002/annotation2477763/range2477763/1
type: Range
persistent identity: range2477763
display id: range2477763
version: 1
direction: direction/forward
top level: BBa_K1689002
start: 1
end: 36
orientation: inline

stop codon
type: SequenceAnnotation
persistent identity: annotation2469952
display id: annotation2469952
version: 1
top level: BBa_K1689002
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1689002/annotation2469952/range2469952/1

https://synbiohub.org/public/igem/BBa_K1689002/annotation2469952/range2469952/1
type: Range
persistent identity: range2469952
display id: range2469952
version: 1
direction: direction/forward
top level: BBa_K1689002
start: 880
end: 882
orientation: inline

start codon
type: SequenceAnnotation
persistent identity: annotation2469942
display id: annotation2469942
version: 1
top level: BBa_K1689002
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1689002/annotation2469942/range2469942/1

https://synbiohub.org/public/igem/BBa_K1689002/annotation2469942/range2469942/1
type: Range
persistent identity: range2469942
display id: range2469942
version: 1
direction: direction/forward
top level: BBa_K1689002
start: 37
end: 39
orientation: inline

BBa_K1315012
type: ComponentDefinition
persistent identity: BBa K1315012
display id: BBa_K1315012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pseudomonas AutoInducer-1 response unit (luxR promoter)
creator: Dimitrios Michailidis
created: 2014-09-26T11:00:00Z
modified: 2015-05-08T01:09:50Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1690_
m user id: 0
owner id: 21812
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: ptet + lasR + pluxR
mutable notes: All the inserts were digested with XbaI and PstI, and all the vectors were each time cut with SpeI and PstI.
mutable provenance: All the parts have come from existing iGEM distribution kits.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_R0062
component: BBa_C0179
component: BBa_R0040
sequence annotation: BBa_C0179
sequence annotation: BBa_R0062
sequence annotation: BBa_R0040
sequence: https://synbiohub.org/public/igem/BBa_K1315012_sequence/1

BBa_R0062
type: Component
persistent identity: component2387464
display id: component2387464
version: 1
top level: BBa_K1315012
access: public
definition: https://synbiohub.org/public/igem/BBa_R0062/1

BBa_C0179
type: Component
persistent identity: component2387462
display id: component2387462
version: 1
top level: BBa_K1315012
access: public
definition: https://synbiohub.org/public/igem/BBa_C0179/1

BBa_R0040
type: Component
persistent identity: component2387457
display id: component2387457
version: 1
top level: BBa_K1315012
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_C0179
type: SequenceAnnotation
persistent identity: annotation2387462
display id: annotation2387462
version: 1
top level: BBa_K1315012
location: https://synbiohub.org/public/igem/BBa_K1315012/annotation2387462/range2387462/1
component: BBa_C0179

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type: Range
persistent identity: range2387462
display id: range2387462
version: 1
direction: direction/reverse
top level: BBa_K1315012
start: 61
end: 783
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation2387464
display id: annotation2387464
version: 1
top level: BBa_K1315012
location: https://synbiohub.org/public/igem/BBa_K1315012/annotation2387464/range2387464/1
component: BBa_R0062

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type: Range
persistent identity: range2387464
display id: range2387464
version: 1
direction: direction/forward
top level: BBa_K1315012
start: 792
end: 846
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2387457
display id: annotation2387457
version: 1
top level: BBa_K1315012
location: https://synbiohub.org/public/igem/BBa_K1315012/annotation2387457/range2387457/1
component: BBa_R0040

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type: Range
persistent identity: range2387457
display id: range2387457
version: 1
direction: direction/forward
top level: BBa_K1315012
start: 1
end: 54
orientation: inline

BBa_K1321358
type: ComponentDefinition
persistent identity: BBa K1321358
display id: BBa_K1321358
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CBDcenA with linker fused to NiBP driven by LacI
creator: Chris N Micklem
created: 2014-10-07T11:00:00Z
modified: 2015-05-08T01:09:53Z
discontinued: true
dominant: false
experience: experience/None
group u list: _1696_
m user id: 0
owner id: 20830
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: asdf
mutable notes: asdf
mutable provenance: asdf
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1319106
component: BBa_J04500
component: BBa_B0105
component: BBa_K1321009
component: BBa_K1321339
sequence annotation: BBa_K1321009
sequence annotation: BBa_K1321339
sequence annotation: BBa_B0105
sequence annotation: BBa_J04500
sequence annotation: BBa_K1319106
sequence: https://synbiohub.org/public/igem/BBa_K1321358_sequence/1

BBa_K1319106
type: Component
persistent identity: component2422625
display id: component2422625
version: 1
top level: BBa_K1321358
access: public
definition: https://synbiohub.org/public/igem/BBa_K1319106/1

BBa_J04500
type: Component
persistent identity: component2422623
display id: component2422623
version: 1
top level: BBa_K1321358
access: public
definition: https://synbiohub.org/public/igem/BBa_J04500/1

BBa_B0105
type: Component
persistent identity: component2422630
display id: component2422630
version: 1
top level: BBa_K1321358
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_K1321009
type: Component
persistent identity: component2422632
display id: component2422632
version: 1
top level: BBa_K1321358
access: public
definition: BBa_K1321009

BBa_K1321339
type: Component
persistent identity: component2422628
display id: component2422628
version: 1
top level: BBa_K1321358
access: public
definition: BBa_K1321339

BBa_K1321009
type: SequenceAnnotation
persistent identity: annotation2422632
display id: annotation2422632
version: 1
top level: BBa_K1321358
location: https://synbiohub.org/public/igem/BBa_K1321358/annotation2422632/range2422632/1
component: BBa_K1321009

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type: Range
persistent identity: range2422632
display id: range2422632
version: 1
direction: direction/reverse
top level: BBa_K1321358
start: 650
end: 825
orientation: inline

BBa_K1321339
type: SequenceAnnotation
persistent identity: annotation2422628
display id: annotation2422628
version: 1
top level: BBa_K1321358
location: https://synbiohub.org/public/igem/BBa_K1321358/annotation2422628/range2422628/1
component: BBa_K1321339

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type: Range
persistent identity: range2422628
display id: range2422628
version: 1
direction: direction/reverse
top level: BBa_K1321358
start: 236
end: 643
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2422630
display id: annotation2422630
version: 1
top level: BBa_K1321358
location: https://synbiohub.org/public/igem/BBa_K1321358/annotation2422630/range2422630/1
component: BBa_B0105

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type: Range
persistent identity: range2422630
display id: range2422630
version: 1
direction: direction/reverse
top level: BBa_K1321358
start: 644
end: 649
orientation: inline

BBa_J04500
type: SequenceAnnotation
persistent identity: annotation2422623
display id: annotation2422623
version: 1
top level: BBa_K1321358
location: https://synbiohub.org/public/igem/BBa_K1321358/annotation2422623/range2422623/1
component: BBa_J04500

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type: Range
persistent identity: range2422623
display id: range2422623
version: 1
direction: direction/reverse
top level: BBa_K1321358
start: 1
end: 220
orientation: inline

BBa_K1319106
type: SequenceAnnotation
persistent identity: annotation2422625
display id: annotation2422625
version: 1
top level: BBa_K1321358
location: https://synbiohub.org/public/igem/BBa_K1321358/annotation2422625/range2422625/1
component: BBa_K1319106

https://synbiohub.org/public/igem/BBa_K1321358/annotation2422625/range2422625/1
type: Range
persistent identity: range2422625
display id: range2422625
version: 1
direction: direction/reverse
top level: BBa_K1321358
start: 221
end: 235
orientation: inline

BBa_K1850011
type: ComponentDefinition
persistent identity: BBa K1850011
display id: BBa_K1850011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pRha - fimH 49 KO - RPMrel - SpyTag_225 - HisTag_225
creator: Lydia Goldberg
created: 2015-09-14T11:00:00Z
modified: 2015-09-16T10:48:20Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2277_
m user id: 26421
owner id: 26421
owning group id: 9
status: status/Planning
bookmark: false
mutable description: placeholder
mutable notes: placeholder
mutable provenance: placeholder
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: RPMrel
sequence annotation: SpyTag
sequence annotation: fimH
sequence annotation: B0034
sequence annotation: pRha
sequence annotation: HisTag
sequence: https://synbiohub.org/public/igem/BBa_K1850011_sequence/1

RPMrel
type: SequenceAnnotation
persistent identity: annotation2463796
display id: annotation2463796
version: 1
top level: BBa_K1850011
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1850011/annotation2463796/range2463796/1

https://synbiohub.org/public/igem/BBa_K1850011/annotation2463796/range2463796/1
type: Range
persistent identity: range2463796
display id: range2463796
version: 1
direction: direction/forward
top level: BBa_K1850011
start: 333
end: 359
orientation: inline

SpyTag
type: SequenceAnnotation
persistent identity: annotation2463798
display id: annotation2463798
version: 1
top level: BBa_K1850011
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1850011/annotation2463798/range2463798/1

https://synbiohub.org/public/igem/BBa_K1850011/annotation2463798/range2463798/1
type: Range
persistent identity: range2463798
display id: range2463798
version: 1
direction: direction/forward
top level: BBa_K1850011
start: 1032
end: 1070
orientation: inline

fimH
type: SequenceAnnotation
persistent identity: annotation2463795
display id: annotation2463795
version: 1
top level: BBa_K1850011
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1850011/annotation2463795/range2463795/1

https://synbiohub.org/public/igem/BBa_K1850011/annotation2463795/range2463795/1
type: Range
persistent identity: range2463795
display id: range2463795
version: 1
direction: direction/forward
top level: BBa_K1850011
start: 270
end: 1256
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2463794
display id: annotation2463794
version: 1
top level: BBa_K1850011
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1850011/annotation2463794/range2463794/1

https://synbiohub.org/public/igem/BBa_K1850011/annotation2463794/range2463794/1
type: Range
persistent identity: range2463794
display id: range2463794
version: 1
direction: direction/forward
top level: BBa_K1850011
start: 252
end: 263
orientation: inline

pRha
type: SequenceAnnotation
persistent identity: annotation2463792
display id: annotation2463792
version: 1
top level: BBa_K1850011
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1850011/annotation2463792/range2463792/1

https://synbiohub.org/public/igem/BBa_K1850011/annotation2463792/range2463792/1
type: Range
persistent identity: range2463792
display id: range2463792
version: 1
direction: direction/forward
top level: BBa_K1850011
start: 4
end: 243
orientation: inline

HisTag
type: SequenceAnnotation
persistent identity: annotation2463799
display id: annotation2463799
version: 1
top level: BBa_K1850011
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1850011/annotation2463799/range2463799/1

https://synbiohub.org/public/igem/BBa_K1850011/annotation2463799/range2463799/1
type: Range
persistent identity: range2463799
display id: range2463799
version: 1
direction: direction/forward
top level: BBa_K1850011
start: 1071
end: 1088
orientation: inline

BBa_K1692001
type: ComponentDefinition
persistent identity: BBa K1692001
display id: BBa_K1692001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ferulic acid decarboxylase (FDC) with T7 promoter and RBS
creator: Daniel Kunin
created: 2015-08-02T11:00:00Z
modified: 2015-08-03T12:51:21Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2110_
m user id: 26209
owner id: 26209
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Ferulic acid decarboxylase (FDC) with T7 promoter and RBS
mutable notes: Site directed mutagenesis of SpeI restriction enzyme site within sequence.
mutable provenance: Saccharomyces cerevisiae
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1692000
component: BBa_K525998
sequence annotation: BBa_K1692000
sequence annotation: BBa_K525998
sequence: https://synbiohub.org/public/igem/BBa_K1692001_sequence/1

BBa_K1692000
type: Component
persistent identity: component2433977
display id: component2433977
version: 1
top level: BBa_K1692001
access: public
definition: https://synbiohub.org/public/igem/BBa_K1692000/1

BBa_K525998
type: Component
persistent identity: component2433976
display id: component2433976
version: 1
top level: BBa_K1692001
access: public
definition: https://synbiohub.org/public/igem/BBa_K525998/1

BBa_K1692000
type: SequenceAnnotation
persistent identity: annotation2433977
display id: annotation2433977
version: 1
top level: BBa_K1692001
location: https://synbiohub.org/public/igem/BBa_K1692001/annotation2433977/range2433977/1
component: BBa_K1692000

https://synbiohub.org/public/igem/BBa_K1692001/annotation2433977/range2433977/1
type: Range
persistent identity: range2433977
display id: range2433977
version: 1
direction: direction/reverse
top level: BBa_K1692001
start: 39
end: 1550
orientation: inline

BBa_K525998
type: SequenceAnnotation
persistent identity: annotation2433976
display id: annotation2433976
version: 1
top level: BBa_K1692001
location: https://synbiohub.org/public/igem/BBa_K1692001/annotation2433976/range2433976/1
component: BBa_K525998

https://synbiohub.org/public/igem/BBa_K1692001/annotation2433976/range2433976/1
type: Range
persistent identity: range2433976
display id: range2433976
version: 1
direction: direction/reverse
top level: BBa_K1692001
start: 1
end: 32
orientation: inline

BBa_K1585212
type: ComponentDefinition
persistent identity: BBa K1585212
display id: BBa_K1585212
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0034.PHI
creator: Laura Grabowski
created: 2015-08-24T11:00:00Z
modified: 2015-08-28T08:24:03Z
discontinued: false
dominant: false
group u list: _2002_
m user id: 19522
owner id: 24505
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This is the translational unit of the 6-phospho-3-hexuloisomerase (PHI) from Bacillus methanolicus MGA3. It is codon optimized for E.coli.
mutable notes: RFC10 compatible
mutable provenance: Bacillus methanolicus
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0034
component: BBa_K1585202
sequence annotation: BBa_K1585202
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K1585212_sequence/1

BBa_B0034
type: Component
persistent identity: component2439455
display id: component2439455
version: 1
top level: BBa_K1585212
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1585202
type: Component
persistent identity: component2439456
display id: component2439456
version: 1
top level: BBa_K1585212
access: public
definition: https://synbiohub.org/public/igem/BBa_K1585202/1

BBa_K1585202
type: SequenceAnnotation
persistent identity: annotation2439456
display id: annotation2439456
version: 1
top level: BBa_K1585212
location: https://synbiohub.org/public/igem/BBa_K1585212/annotation2439456/range2439456/1
component: BBa_K1585202

https://synbiohub.org/public/igem/BBa_K1585212/annotation2439456/range2439456/1
type: Range
persistent identity: range2439456
display id: range2439456
version: 1
direction: direction/reverse
top level: BBa_K1585212
start: 19
end: 576
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2439455
display id: annotation2439455
version: 1
top level: BBa_K1585212
location: https://synbiohub.org/public/igem/BBa_K1585212/annotation2439455/range2439455/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1585212/annotation2439455/range2439455/1
type: Range
persistent identity: range2439455
display id: range2439455
version: 1
direction: direction/reverse
top level: BBa_K1585212
start: 1
end: 12
orientation: inline

BBa_K1800002
type: ComponentDefinition
persistent identity: BBa K1800002
display id: BBa_K1800002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Horseradish Peroxidase
creator: Laura Lee Irvin
created: 2015-08-27T11:00:00Z
modified: 2015-12-16T01:32:07Z
discontinued: false
dominant: false
group u list: _2225_
m user id: 4206
owner id: 27447
owning group id: 9
status: status/Planning
bookmark: false
mutable description: In an effort to show proof of concept for the CBDA synthase portion of the project, the GSU-iGem team is using horseradish peroxidase transformed into agrobacterium then producing transgenic tobacco plants.
mutable notes: It is intended to be put into pGAP vectors and pORE vectors for expression in Pichia Pastoris and Agrobacterium.
mutable provenance: synthesized by IDT
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1800002_sequence/1

BBa_K1632008
type: ComponentDefinition
persistent identity: BBa K1632008
display id: BBa_K1632008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: fim switch[default OFF](wild-type)_rbs_gfp
creator: Riku Shinohara
created: 2015-09-09T11:00:00Z
modified: 2015-09-15T07:04:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2049_
m user id: 24760
owner id: 22687
owning group id: 9
status: status/Planning
bookmark: false
mutable description: a
mutable notes: 1
mutable provenance: a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1632005
component: BBa_I13500
sequence annotation: BBa_K1632005
sequence annotation: BBa_I13500
sequence: https://synbiohub.org/public/igem/BBa_K1632008_sequence/1

BBa_K1632005
type: Component
persistent identity: component2448481
display id: component2448481
version: 1
top level: BBa_K1632008
access: public
definition: BBa_K1632005

BBa_I13500
type: Component
persistent identity: component2448485
display id: component2448485
version: 1
top level: BBa_K1632008
access: public
definition: https://synbiohub.org/public/igem/BBa_I13500/1

BBa_K1632005
type: SequenceAnnotation
persistent identity: annotation2448481
display id: annotation2448481
version: 1
top level: BBa_K1632008
location: https://synbiohub.org/public/igem/BBa_K1632008/annotation2448481/range2448481/1
component: BBa_K1632005

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type: Range
persistent identity: range2448481
display id: range2448481
version: 1
direction: direction/reverse
top level: BBa_K1632008
start: 1
end: 382
orientation: inline

BBa_I13500
type: SequenceAnnotation
persistent identity: annotation2448485
display id: annotation2448485
version: 1
top level: BBa_K1632008
location: https://synbiohub.org/public/igem/BBa_K1632008/annotation2448485/range2448485/1
component: BBa_I13500

https://synbiohub.org/public/igem/BBa_K1632008/annotation2448485/range2448485/1
type: Range
persistent identity: range2448485
display id: range2448485
version: 1
direction: direction/reverse
top level: BBa_K1632008
start: 391
end: 1128
orientation: inline

BBa_K1758301
type: ComponentDefinition
persistent identity: BBa K1758301
display id: BBa_K1758301
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: His-tagged arsenic repressor (ArsR) under control of the T7 promoter
creator: Team Bielefeld-CeBiTec 2015
created: 2015-08-21T11:00:00Z
modified: 2015-09-18T04:46:36Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2180_
m user id: 27935
owner id: 26587
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This part can be used for expression and purification of the arsenic repressor ArsR.
mutable notes: Created using Gibson Assembly.
mutable provenance: Based on BBa_J33201.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: arsR
sequence annotation: T7 promoter + RBS
sequence annotation: stop
sequence annotation: his-tag
sequence: https://synbiohub.org/public/igem/BBa_K1758301_sequence/1

arsR
type: SequenceAnnotation
persistent identity: annotation2436873
display id: annotation2436873
version: 1
top level: BBa_K1758301
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1758301/annotation2436873/range2436873/1

https://synbiohub.org/public/igem/BBa_K1758301/annotation2436873/range2436873/1
type: Range
persistent identity: range2436873
display id: range2436873
version: 1
direction: direction/forward
top level: BBa_K1758301
start: 41
end: 391
orientation: inline

T7 promoter + RBS
type: SequenceAnnotation
persistent identity: annotation2436876
display id: annotation2436876
version: 1
top level: BBa_K1758301
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1758301/annotation2436876/range2436876/1

https://synbiohub.org/public/igem/BBa_K1758301/annotation2436876/range2436876/1
type: Range
persistent identity: range2436876
display id: range2436876
version: 1
direction: direction/forward
top level: BBa_K1758301
start: 1
end: 32
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2436875
display id: annotation2436875
version: 1
top level: BBa_K1758301
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1758301/annotation2436875/range2436875/1

https://synbiohub.org/public/igem/BBa_K1758301/annotation2436875/range2436875/1
type: Range
persistent identity: range2436875
display id: range2436875
version: 1
direction: direction/forward
top level: BBa_K1758301
start: 410
end: 412
orientation: inline

his-tag
type: SequenceAnnotation
persistent identity: annotation2436874
display id: annotation2436874
version: 1
top level: BBa_K1758301
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1758301/annotation2436874/range2436874/1

https://synbiohub.org/public/igem/BBa_K1758301/annotation2436874/range2436874/1
type: Range
persistent identity: range2436874
display id: range2436874
version: 1
direction: direction/forward
top level: BBa_K1758301
start: 392
end: 409
orientation: inline

BBa_K1362160
type: ComponentDefinition
persistent identity: BBa K1362160
display id: BBa_K1362160
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS + Gp41-1 N-intein RFC[105] assembly construct (with His6)
creator: Jan Gleixner
created: 2014-10-09T11:00:00Z
modified: 2015-05-08T01:10:05Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1738_
m user id: 0
owner id: 22830
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1362422
component: BBa_K1362421
component: BBa_K1362414
component: BBa_K1362415
component: BBa_K1362090
component: BBa_K1362412
component: BBa_G0000
component: BBa_K1362430
component: BBa_K1362437
component: BBa_K1362417
sequence annotation: BBa_K1362415
sequence annotation: BBa_K1362437
sequence annotation: BBa_G0000
sequence annotation: BBa_K1362414
sequence annotation: BBa_K1362417
sequence annotation: BBa_K1362090
sequence annotation: BBa_K1362422
sequence annotation: BBa_K1362412
sequence annotation: BBa_K1362430
sequence annotation: BBa_K1362421
sequence: https://synbiohub.org/public/igem/BBa_K1362160_sequence/1

BBa_K1362422
type: Component
persistent identity: component2413349
display id: component2413349
version: 1
top level: BBa_K1362160
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362422/1

BBa_K1362421
type: Component
persistent identity: component2413359
display id: component2413359
version: 1
top level: BBa_K1362160
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362421/1

BBa_K1362414
type: Component
persistent identity: component2413328
display id: component2413328
version: 1
top level: BBa_K1362160
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362414/1

BBa_K1362415
type: Component
persistent identity: component2413350
display id: component2413350
version: 1
top level: BBa_K1362160
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362415/1

BBa_K1362090
type: Component
persistent identity: component2413326
display id: component2413326
version: 1
top level: BBa_K1362160
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362090/1

BBa_K1362412
type: Component
persistent identity: component2413351
display id: component2413351
version: 1
top level: BBa_K1362160
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362412/1

BBa_G0000
type: Component
persistent identity: component2413327
display id: component2413327
version: 1
top level: BBa_K1362160
access: public
definition: https://synbiohub.org/public/igem/BBa_G0000/1

BBa_K1362430
type: Component
persistent identity: component2413357
display id: component2413357
version: 1
top level: BBa_K1362160
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362430/1

BBa_K1362437
type: Component
persistent identity: component2413361
display id: component2413361
version: 1
top level: BBa_K1362160
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362437/1

BBa_K1362417
type: Component
persistent identity: component2413354
display id: component2413354
version: 1
top level: BBa_K1362160
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362417/1

BBa_K1362415
type: SequenceAnnotation
persistent identity: annotation2413350
display id: annotation2413350
version: 1
top level: BBa_K1362160
location: https://synbiohub.org/public/igem/BBa_K1362160/annotation2413350/range2413350/1
component: BBa_K1362415

https://synbiohub.org/public/igem/BBa_K1362160/annotation2413350/range2413350/1
type: Range
persistent identity: range2413350
display id: range2413350
version: 1
direction: direction/reverse
top level: BBa_K1362160
start: 1122
end: 1125
orientation: inline

BBa_K1362437
type: SequenceAnnotation
persistent identity: annotation2413361
display id: annotation2413361
version: 1
top level: BBa_K1362160
location: https://synbiohub.org/public/igem/BBa_K1362160/annotation2413361/range2413361/1
component: BBa_K1362437

https://synbiohub.org/public/igem/BBa_K1362160/annotation2413361/range2413361/1
type: Range
persistent identity: range2413361
display id: range2413361
version: 1
direction: direction/reverse
top level: BBa_K1362160
start: 1420
end: 1422
orientation: inline

BBa_G0000
type: SequenceAnnotation
persistent identity: annotation2413327
display id: annotation2413327
version: 1
top level: BBa_K1362160
location: https://synbiohub.org/public/igem/BBa_K1362160/annotation2413327/range2413327/1
component: BBa_G0000

https://synbiohub.org/public/igem/BBa_K1362160/annotation2413327/range2413327/1
type: Range
persistent identity: range2413327
display id: range2413327
version: 1
direction: direction/reverse
top level: BBa_K1362160
start: 30
end: 35
orientation: inline

BBa_K1362414
type: SequenceAnnotation
persistent identity: annotation2413328
display id: annotation2413328
version: 1
top level: BBa_K1362160
location: https://synbiohub.org/public/igem/BBa_K1362160/annotation2413328/range2413328/1
component: BBa_K1362414

https://synbiohub.org/public/igem/BBa_K1362160/annotation2413328/range2413328/1
type: Range
persistent identity: range2413328
display id: range2413328
version: 1
direction: direction/reverse
top level: BBa_K1362160
start: 36
end: 38
orientation: inline

BBa_K1362417
type: SequenceAnnotation
persistent identity: annotation2413354
display id: annotation2413354
version: 1
top level: BBa_K1362160
location: https://synbiohub.org/public/igem/BBa_K1362160/annotation2413354/range2413354/1
component: BBa_K1362417

https://synbiohub.org/public/igem/BBa_K1362160/annotation2413354/range2413354/1
type: Range
persistent identity: range2413354
display id: range2413354
version: 1
direction: direction/reverse
top level: BBa_K1362160
start: 1386
end: 1391
orientation: inline

BBa_K1362090
type: SequenceAnnotation
persistent identity: annotation2413326
display id: annotation2413326
version: 1
top level: BBa_K1362160
location: https://synbiohub.org/public/igem/BBa_K1362160/annotation2413326/range2413326/1
component: BBa_K1362090

https://synbiohub.org/public/igem/BBa_K1362160/annotation2413326/range2413326/1
type: Range
persistent identity: range2413326
display id: range2413326
version: 1
direction: direction/reverse
top level: BBa_K1362160
start: 1
end: 29
orientation: inline

BBa_K1362422
type: SequenceAnnotation
persistent identity: annotation2413349
display id: annotation2413349
version: 1
top level: BBa_K1362160
location: https://synbiohub.org/public/igem/BBa_K1362160/annotation2413349/range2413349/1
component: BBa_K1362422

https://synbiohub.org/public/igem/BBa_K1362160/annotation2413349/range2413349/1
type: Range
persistent identity: range2413349
display id: range2413349
version: 1
direction: direction/reverse
top level: BBa_K1362160
start: 39
end: 1121
orientation: inline

BBa_K1362412
type: SequenceAnnotation
persistent identity: annotation2413351
display id: annotation2413351
version: 1
top level: BBa_K1362160
location: https://synbiohub.org/public/igem/BBa_K1362160/annotation2413351/range2413351/1
component: BBa_K1362412

https://synbiohub.org/public/igem/BBa_K1362160/annotation2413351/range2413351/1
type: Range
persistent identity: range2413351
display id: range2413351
version: 1
direction: direction/reverse
top level: BBa_K1362160
start: 1126
end: 1385
orientation: inline

BBa_K1362430
type: SequenceAnnotation
persistent identity: annotation2413357
display id: annotation2413357
version: 1
top level: BBa_K1362160
location: https://synbiohub.org/public/igem/BBa_K1362160/annotation2413357/range2413357/1
component: BBa_K1362430

https://synbiohub.org/public/igem/BBa_K1362160/annotation2413357/range2413357/1
type: Range
persistent identity: range2413357
display id: range2413357
version: 1
direction: direction/reverse
top level: BBa_K1362160
start: 1392
end: 1415
orientation: inline

BBa_K1362421
type: SequenceAnnotation
persistent identity: annotation2413359
display id: annotation2413359
version: 1
top level: BBa_K1362160
location: https://synbiohub.org/public/igem/BBa_K1362160/annotation2413359/range2413359/1
component: BBa_K1362421

https://synbiohub.org/public/igem/BBa_K1362160/annotation2413359/range2413359/1
type: Range
persistent identity: range2413359
display id: range2413359
version: 1
direction: direction/reverse
top level: BBa_K1362160
start: 1416
end: 1419
orientation: inline

BBa_K1336003
type: ComponentDefinition
persistent identity: BBa K1336003
display id: BBa_K1336003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Dye-decolourising peroxidase from Bacillus subtilis
creator: Yan-Kay Ho
created: 2014-10-05T11:00:00Z
modified: 2015-05-08T01:09:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1711_
m user id: 0
owner id: 20842
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: BsDyP gene (1251bp) from Bacillus subtilis that has been found to be effective in degrading lignin and azo dyes, in an oxidative process.
mutable notes: None.
mutable provenance: From genomic sequence of Bacillus subtilis; BsDyP gene inserted into a pET-21a (+) expression vector, provided to us by XXX Research Group.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: BsDyP coding region
sequence annotation: TAA stop codon
sequence annotation: ATG start codon
sequence: https://synbiohub.org/public/igem/BBa_K1336003_sequence/1

BsDyP coding region
type: SequenceAnnotation
persistent identity: annotation2397241
display id: annotation2397241
version: 1
top level: BBa_K1336003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1336003/annotation2397241/range2397241/1

https://synbiohub.org/public/igem/BBa_K1336003/annotation2397241/range2397241/1
type: Range
persistent identity: range2397241
display id: range2397241
version: 1
direction: direction/forward
top level: BBa_K1336003
start: 1
end: 1251
orientation: inline

TAA stop codon
type: SequenceAnnotation
persistent identity: annotation2397243
display id: annotation2397243
version: 1
top level: BBa_K1336003
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1336003/annotation2397243/range2397243/1

https://synbiohub.org/public/igem/BBa_K1336003/annotation2397243/range2397243/1
type: Range
persistent identity: range2397243
display id: range2397243
version: 1
direction: direction/forward
top level: BBa_K1336003
start: 1249
end: 1251
orientation: inline

ATG start codon
type: SequenceAnnotation
persistent identity: annotation2397242
display id: annotation2397242
version: 1
top level: BBa_K1336003
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1336003/annotation2397242/range2397242/1

https://synbiohub.org/public/igem/BBa_K1336003/annotation2397242/range2397242/1
type: Range
persistent identity: range2397242
display id: range2397242
version: 1
direction: direction/forward
top level: BBa_K1336003
start: 1
end: 3
orientation: inline

BBa_K1362004
type: ComponentDefinition
persistent identity: BBa K1362004
display id: BBa_K1362004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Sortase A non-circularization construct (with His6)
creator: Silvan Schmitz
created: 2014-10-08T11:00:00Z
modified: 2015-05-08T01:10:04Z
discontinued: false
dominant: false
group u list: _1738_
m user id: 0
owner id: 22951
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: TODO
mutable notes: TODO
mutable provenance: TODO
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1362204
component: BBa_K1362090
sequence annotation: BBa_K1362204
sequence annotation: BBa_K1362090
sequence: https://synbiohub.org/public/igem/BBa_K1362004_sequence/1

BBa_K1362204
type: Component
persistent identity: component2409483
display id: component2409483
version: 1
top level: BBa_K1362004
access: public
definition: BBa_K1362204

BBa_K1362090
type: Component
persistent identity: component2409455
display id: component2409455
version: 1
top level: BBa_K1362004
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362090/1

BBa_K1362204
type: SequenceAnnotation
persistent identity: annotation2409483
display id: annotation2409483
version: 1
top level: BBa_K1362004
location: https://synbiohub.org/public/igem/BBa_K1362004/annotation2409483/range2409483/1
component: BBa_K1362204

https://synbiohub.org/public/igem/BBa_K1362004/annotation2409483/range2409483/1
type: Range
persistent identity: range2409483
display id: range2409483
version: 1
direction: direction/reverse
top level: BBa_K1362004
start: 36
end: 1195
orientation: inline

BBa_K1362090
type: SequenceAnnotation
persistent identity: annotation2409455
display id: annotation2409455
version: 1
top level: BBa_K1362004
location: https://synbiohub.org/public/igem/BBa_K1362004/annotation2409455/range2409455/1
component: BBa_K1362090

https://synbiohub.org/public/igem/BBa_K1362004/annotation2409455/range2409455/1
type: Range
persistent identity: range2409455
display id: range2409455
version: 1
direction: direction/reverse
top level: BBa_K1362004
start: 1
end: 29
orientation: inline

BBa_K1321122
type: ComponentDefinition
persistent identity: BBa K1321122
display id: BBa_K1321122
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Smt fused to CBDcex
creator: Gabriella Santosa
created: 2014-10-08T11:00:00Z
modified: 2015-06-17T12:22:45Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 4206
owner id: 20961
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: GATC
mutable notes: GATC
mutable provenance: GATC
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_B0105
component: BBa_K1321011
component: BBa_K1321003
sequence annotation: BBa_K1321003
sequence annotation: BBa_B0105
sequence annotation: BBa_K1321011
sequence: https://synbiohub.org/public/igem/BBa_K1321122_sequence/1

BBa_B0105
type: Component
persistent identity: component2414990
display id: component2414990
version: 1
top level: BBa_K1321122
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_K1321011
type: Component
persistent identity: component2414988
display id: component2414988
version: 1
top level: BBa_K1321122
access: public
definition: https://synbiohub.org/public/igem/BBa_K1321011/1

BBa_K1321003
type: Component
persistent identity: component2414991
display id: component2414991
version: 1
top level: BBa_K1321122
access: public
definition: https://synbiohub.org/public/igem/BBa_K1321003/1

BBa_K1321003
type: SequenceAnnotation
persistent identity: annotation2414991
display id: annotation2414991
version: 1
top level: BBa_K1321122
location: https://synbiohub.org/public/igem/BBa_K1321122/annotation2414991/range2414991/1
component: BBa_K1321003

https://synbiohub.org/public/igem/BBa_K1321122/annotation2414991/range2414991/1
type: Range
persistent identity: range2414991
display id: range2414991
version: 1
direction: direction/reverse
top level: BBa_K1321122
start: 172
end: 498
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2414990
display id: annotation2414990
version: 1
top level: BBa_K1321122
location: https://synbiohub.org/public/igem/BBa_K1321122/annotation2414990/range2414990/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_K1321122/annotation2414990/range2414990/1
type: Range
persistent identity: range2414990
display id: range2414990
version: 1
direction: direction/reverse
top level: BBa_K1321122
start: 166
end: 171
orientation: inline

BBa_K1321011
type: SequenceAnnotation
persistent identity: annotation2414988
display id: annotation2414988
version: 1
top level: BBa_K1321122
location: https://synbiohub.org/public/igem/BBa_K1321122/annotation2414988/range2414988/1
component: BBa_K1321011

https://synbiohub.org/public/igem/BBa_K1321122/annotation2414988/range2414988/1
type: Range
persistent identity: range2414988
display id: range2414988
version: 1
direction: direction/reverse
top level: BBa_K1321122
start: 1
end: 165
orientation: inline

mLacI
type: ComponentDefinition
persistent identity: BBa K1639011
display id: BBa_K1639011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: mammalian LacI with miR-223 and miR-21 binding sites
creator: Nurgeldi Bazarov
created: 2015-09-14T11:00:00Z
modified: 2015-09-15T12:46:07Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2056_
m user id: 24789
owner id: 24789
owning group id: 9
status: status/Planning
bookmark: false
mutable description: In our project second step of cancer module is based on production of LacI so that it will suppress expression of DsRed. Binding site on 3' end of LacI makes it regulatable and limits it's expression only in cells with low miR-223 and miR-21 levels. miR-21 has high rate of production in most cancer types. In gastric epithelial cells level of this miRNAs are low but in gastric cancer cells their level is very high.
mutable notes: We suggest also to view similar part we submitted-BBa_K1639010. It contains mLacI with miR binding sites but have restriction enzyme cut sites to remove miR binding site as needed.
mutable provenance: This protein is mammalian codon optimized version of LacI repressor originally derived from E.coli. It also possess nuclear localization signal (NLS) so that it can act on lac operators in nucleus.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Kozak
sequence annotation: Stop Codon
sequence annotation: SV40 NLS
sequence annotation: ATG
sequence annotation: miR-223 binding site
sequence annotation: mLacI
sequence annotation: miR-21 binding site
sequence: https://synbiohub.org/public/igem/BBa_K1639011_sequence/1

Kozak
type: SequenceAnnotation
persistent identity: annotation2457408
display id: annotation2457408
version: 1
top level: mLacI
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1639011/annotation2457408/range2457408/1

https://synbiohub.org/public/igem/BBa_K1639011/annotation2457408/range2457408/1
type: Range
persistent identity: range2457408
display id: range2457408
version: 1
direction: direction/forward
top level: mLacI
start: 1
end: 6
orientation: inline

Stop Codon
type: SequenceAnnotation
persistent identity: annotation2457427
display id: annotation2457427
version: 1
top level: mLacI
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1639011/annotation2457427/range2457427/1

https://synbiohub.org/public/igem/BBa_K1639011/annotation2457427/range2457427/1
type: Range
persistent identity: range2457427
display id: range2457427
version: 1
direction: direction/forward
top level: mLacI
start: 1123
end: 1128
orientation: inline

SV40 NLS
type: SequenceAnnotation
persistent identity: annotation2457422
display id: annotation2457422
version: 1
top level: mLacI
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1639011/annotation2457422/range2457422/1

https://synbiohub.org/public/igem/BBa_K1639011/annotation2457422/range2457422/1
type: Range
persistent identity: range2457422
display id: range2457422
version: 1
direction: direction/forward
top level: mLacI
start: 1102
end: 1122
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2457409
display id: annotation2457409
version: 1
top level: mLacI
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1639011/annotation2457409/range2457409/1

https://synbiohub.org/public/igem/BBa_K1639011/annotation2457409/range2457409/1
type: Range
persistent identity: range2457409
display id: range2457409
version: 1
direction: direction/forward
top level: mLacI
start: 7
end: 9
orientation: inline

miR-223 binding site
type: SequenceAnnotation
persistent identity: annotation2457428
display id: annotation2457428
version: 1
top level: mLacI
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1639011/annotation2457428/range2457428/1

https://synbiohub.org/public/igem/BBa_K1639011/annotation2457428/range2457428/1
type: Range
persistent identity: range2457428
display id: range2457428
version: 1
direction: direction/forward
top level: mLacI
start: 1129
end: 1150
orientation: inline

mLacI
type: SequenceAnnotation
persistent identity: annotation2457411
display id: annotation2457411
version: 1
top level: mLacI
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1639011/annotation2457411/range2457411/1

https://synbiohub.org/public/igem/BBa_K1639011/annotation2457411/range2457411/1
type: Range
persistent identity: range2457411
display id: range2457411
version: 1
direction: direction/forward
top level: mLacI
start: 7
end: 1125
orientation: inline

miR-21 binding site
type: SequenceAnnotation
persistent identity: annotation2457429
display id: annotation2457429
version: 1
top level: mLacI
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1639011/annotation2457429/range2457429/1

https://synbiohub.org/public/igem/BBa_K1639011/annotation2457429/range2457429/1
type: Range
persistent identity: range2457429
display id: range2457429
version: 1
direction: direction/forward
top level: mLacI
start: 1180
end: 1200
orientation: inline

BBa_K1758204
type: ComponentDefinition
persistent identity: BBa K1758204
display id: BBa_K1758204
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: His-tagged blc repressor fused to superfolder GFP under the control of the T7 promoter
creator: Team Bielefeld-CeBiTec 2015
created: 2015-08-28T11:00:00Z
modified: 2015-09-18T07:21:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2180_
m user id: 26913
owner id: 26913
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Uses T7 promotor and a strong rbs (BBa_K525998), the blc repressor linked with superfolding GFP (sfGFP) with a His-Tag. By linking sfGFP we want to detect bound protein via fluorescence.
mutable notes: This is a composite part which enables a high protein expression and an easy purification of blcR fused to superfolding GFP (sfGFP). The green fluorescence of the culture is a sign for protein expression.
mutable provenance: We clone blcR in T7-RBS-sfGFP and added a His-Tag via PCR and Gibson Assembly.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: sfGFP
sequence annotation: BBa_K525998
sequence annotation: RBS
sequence annotation: T7 promoter
sequence annotation: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: blcR
sequence annotation: His-Tag
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_K1758204_sequence/1

sfGFP
type: SequenceAnnotation
persistent identity: annotation2440052
display id: annotation2440052
version: 1
top level: BBa_K1758204
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1758204/annotation2440052/range2440052/1

https://synbiohub.org/public/igem/BBa_K1758204/annotation2440052/range2440052/1
type: Range
persistent identity: range2440052
display id: range2440052
version: 1
direction: direction/forward
top level: BBa_K1758204
start: 881
end: 1594
orientation: inline

BBa_K525998
type: SequenceAnnotation
persistent identity: annotation2440047
display id: annotation2440047
version: 1
top level: BBa_K1758204
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1758204/annotation2440047/range2440047/1

https://synbiohub.org/public/igem/BBa_K1758204/annotation2440047/range2440047/1
type: Range
persistent identity: range2440047
display id: range2440047
version: 1
direction: direction/forward
top level: BBa_K1758204
start: 1
end: 32
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2440046
display id: annotation2440046
version: 1
top level: BBa_K1758204
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1758204/annotation2440046/range2440046/1

https://synbiohub.org/public/igem/BBa_K1758204/annotation2440046/range2440046/1
type: Range
persistent identity: range2440046
display id: range2440046
version: 1
direction: direction/forward
top level: BBa_K1758204
start: 24
end: 27
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2440045
display id: annotation2440045
version: 1
top level: BBa_K1758204
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1758204/annotation2440045/range2440045/1

https://synbiohub.org/public/igem/BBa_K1758204/annotation2440045/range2440045/1
type: Range
persistent identity: range2440045
display id: range2440045
version: 1
direction: direction/forward
top level: BBa_K1758204
start: 1
end: 23
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2440049
display id: annotation2440049
version: 1
top level: BBa_K1758204
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1758204/annotation2440049/range2440049/1

https://synbiohub.org/public/igem/BBa_K1758204/annotation2440049/range2440049/1
type: Range
persistent identity: range2440049
display id: range2440049
version: 1
direction: direction/forward
top level: BBa_K1758204
start: 1715
end: 1755
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2440048
display id: annotation2440048
version: 1
top level: BBa_K1758204
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1758204/annotation2440048/range2440048/1

https://synbiohub.org/public/igem/BBa_K1758204/annotation2440048/range2440048/1
type: Range
persistent identity: range2440048
display id: range2440048
version: 1
direction: direction/forward
top level: BBa_K1758204
start: 21
end: 32
orientation: inline

blcR
type: SequenceAnnotation
persistent identity: annotation2440051
display id: annotation2440051
version: 1
top level: BBa_K1758204
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1758204/annotation2440051/range2440051/1

https://synbiohub.org/public/igem/BBa_K1758204/annotation2440051/range2440051/1
type: Range
persistent identity: range2440051
display id: range2440051
version: 1
direction: direction/forward
top level: BBa_K1758204
start: 50
end: 874
orientation: inline

His-Tag
type: SequenceAnnotation
persistent identity: annotation2440053
display id: annotation2440053
version: 1
top level: BBa_K1758204
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1758204/annotation2440053/range2440053/1

https://synbiohub.org/public/igem/BBa_K1758204/annotation2440053/range2440053/1
type: Range
persistent identity: range2440053
display id: range2440053
version: 1
direction: direction/forward
top level: BBa_K1758204
start: 1595
end: 1618
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2440050
display id: annotation2440050
version: 1
top level: BBa_K1758204
role: SO:0000804
role: feature/BioBrick
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persistent identity: BBa K1602008
display id: BBa_K1602008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0034+GRE3
creator: Alexandra Goretzki, Tanja Habeck, Nina Kuschik-Maczollek, Laurin Monnheimer, Steven Pilger, Bianca Reisinger, Daniel Stumpf
created: 2015-09-09T11:00:00Z
modified: 2015-09-25T01:21:40Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2019_
m user id: 12512
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status: status/Planning
bookmark: false
mutable description: BLANK
mutable notes: BLANK
mutable provenance: BLANK
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_B0034
component: BBa_K1602004
sequence annotation: BBa_K1602004
sequence annotation: BBa_B0034
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type: Component
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BBa_K1602004
type: Component
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access: public
definition: GRE3

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type: SequenceAnnotation
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type: ComponentDefinition
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display id: BBa_K1632003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: fim switch[default OFF](Tokyo_Tech/J23119)_rbs_gfp
creator: Riku Shinohara
created: 2015-08-12T11:00:00Z
modified: 2015-09-15T06:52:06Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _2049_
m user id: 24760
owner id: 22687
owning group id: 9
status: status/Planning
bookmark: false
mutable description: 1
mutable notes: 1
mutable provenance: 1
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_E0040
component: BBa_B0034
component: BBa_K1632001
sequence annotation: BBa_K1632001
sequence annotation: BBa_E0040
sequence annotation: BBa_B0034
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type: SequenceAnnotation
persistent identity: annotation2435337
display id: annotation2435337
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type: Range
persistent identity: range2435337
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start: 441
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BBa_K1763443
type: ComponentDefinition
persistent identity: BBa K1763443
display id: BBa_K1763443
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: MaSp1/2[1:2]-12(T7)
creator: Vinson Lam
created: 2015-09-14T11:00:00Z
modified: 2015-09-15T09:53:21Z
discontinued: false
dominant: false
group u list: _2185_
m user id: 27136
owner id: 27136
owning group id: 9
status: status/Planning
bookmark: false
mutable description: MaSp1 and MaSp 12-mer block co-polymer under control of T7 promoter and RBS
mutable notes: no.
mutable provenance: N. clavipes
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K525998
component: BBa_K1763442
sequence annotation: BBa_K525998
sequence annotation: BBa_K1763442
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definition: BBa_K1763442

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BBa_K1362059
type: ComponentDefinition
persistent identity: BBa K1362059
display id: BBa_K1362059
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PKI-NES as RFC[105] N-terminal insert
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B??scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch??fer, Carolin Schmelas, Silvan Schmitz, Max Waldha
created: 2014-10-07T11:00:00Z
modified: 2015-05-08T01:10:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1738_
m user id: 0
owner id: 22830
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000324
role: partType/Tag
component: BBa_K1362440
component: BBa_J63007
component: BBa_K1362425
component: BBa_K1362415
component: BBa_K1362423
component: BBa_K1362444
sequence annotation: BBa_K1362415
sequence annotation: BBa_K1362444
sequence annotation: BBa_K1362423
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sequence annotation: BBa_J63007
sequence annotation: BBa_K1362440
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type: Component
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display id: component2409742
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_K1362440/1

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display id: component2409743
version: 1
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definition: https://synbiohub.org/public/igem/BBa_J63007/1

BBa_K1362425
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version: 1
top level: BBa_K1362059
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362425/1

BBa_K1362415
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display id: component2409745
version: 1
top level: BBa_K1362059
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362415/1

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persistent identity: component2409746
display id: component2409746
version: 1
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access: public
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BBa_K1362444
type: Component
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display id: component2409744
version: 1
top level: BBa_K1362059
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362444/1

BBa_K1362415
type: SequenceAnnotation
persistent identity: annotation2409745
display id: annotation2409745
version: 1
top level: BBa_K1362059
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component: BBa_K1362415

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top level: BBa_K1362059
location: https://synbiohub.org/public/igem/BBa_K1362059/annotation2409744/range2409744/1
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display id: range2409744
version: 1
direction: direction/reverse
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type: SequenceAnnotation
persistent identity: annotation2409746
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version: 1
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location: https://synbiohub.org/public/igem/BBa_K1362059/annotation2409746/range2409746/1
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type: Range
persistent identity: range2409746
display id: range2409746
version: 1
direction: direction/reverse
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start: 63
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type: SequenceAnnotation
persistent identity: annotation2409741
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version: 1
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BBa_J63007
type: SequenceAnnotation
persistent identity: annotation2409743
display id: annotation2409743
version: 1
top level: BBa_K1362059
location: https://synbiohub.org/public/igem/BBa_K1362059/annotation2409743/range2409743/1
component: BBa_J63007

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type: Range
persistent identity: range2409743
display id: range2409743
version: 1
direction: direction/reverse
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start: 11
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type: SequenceAnnotation
persistent identity: annotation2409742
display id: annotation2409742
version: 1
top level: BBa_K1362059
location: https://synbiohub.org/public/igem/BBa_K1362059/annotation2409742/range2409742/1
component: BBa_K1362440

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type: Range
persistent identity: range2409742
display id: range2409742
version: 1
direction: direction/reverse
top level: BBa_K1362059
start: 7
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orientation: inline

BBa_K1365020
type: ComponentDefinition
persistent identity: BBa K1365020
display id: BBa_K1365020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: sfGFP(Bs)
creator: Sandra Mous
created: 2014-09-23T11:00:00Z
modified: 2015-05-08T01:10:07Z
discontinued: false
dominant: false
group u list: _1741_
m user id: 0
owner id: 23000
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Codes for the sfGFP protein, an optimized fluorescent reporter. Was originally optimized for Bacillus subtilis, but then showed high fluorescence in Lactococcus lactis.
mutable notes: Used primers: forward - 5'-GTTTCTTCGAATTCGCGGCCGCTTCTAGATGTCAAAAGGAGAAGAGCT-3' reverse - 5'-GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTACTTATAAAGCTCAT-3' to PCR sfGFP(Bs) from the plasmid and add the BioBrick prefix and suffix.
mutable provenance: PCR on plasmid MG_sfgfp(Bs), used in the study 'Benchmarking Various Green Fluorescent Protein Variants in Bacillus subtilis, Streptococcus pneumoniae, and Lactococcus lactis for Live Cell Imaging', see references. Plasmid kindly provided by the Molecular Genetics group of the Rijksuniversiteit Groningen.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: stop
sequence annotation: sfGFP(Bs)
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_K1365020_sequence/1

stop
type: SequenceAnnotation
persistent identity: annotation2420001
display id: annotation2420001
version: 1
top level: BBa_K1365020
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1365020/annotation2420001/range2420001/1

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type: Range
persistent identity: range2420001
display id: range2420001
version: 1
direction: direction/forward
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start: 715
end: 717
orientation: inline

sfGFP(Bs)
type: SequenceAnnotation
persistent identity: annotation2420000
display id: annotation2420000
version: 1
top level: BBa_K1365020
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1365020/annotation2420000/range2420000/1

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type: Range
persistent identity: range2420000
display id: range2420000
version: 1
direction: direction/forward
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start: 1
end: 717
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2419999
display id: annotation2419999
version: 1
top level: BBa_K1365020
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1365020/annotation2419999/range2419999/1

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type: Range
persistent identity: range2419999
display id: range2419999
version: 1
direction: direction/forward
top level: BBa_K1365020
start: 1
end: 1
orientation: inline

BBa_K1789012
type: ComponentDefinition
persistent identity: BBa K1789012
display id: BBa_K1789012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: P+R
creator: Jiaqi Sun
created: 2015-09-13T11:00:00Z
modified: 2015-09-14T07:22:47Z
discontinued: false
dominant: false
group u list: _2214_
m user id: 26298
owner id: 26298
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This part is a promoter. It has a strong RBS restriction site following it which can guarantee the high efficiency of our project.
mutable notes: We want to construct a promoter which has RBS30 itself for RBS30 is small. If we supply a promoter with RBS, the efficiency of building will be high.
mutable provenance: It is formed by our basic parts.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_R0010
component: BBa_K1789002
sequence annotation: BBa_K1789002
sequence annotation: BBa_R0010
sequence: https://synbiohub.org/public/igem/BBa_K1789012_sequence/1

BBa_R0010
type: Component
persistent identity: component2454122
display id: component2454122
version: 1
top level: BBa_K1789012
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_K1789002
type: Component
persistent identity: component2454130
display id: component2454130
version: 1
top level: BBa_K1789012
access: public
definition: BBa_K1789002

BBa_K1789002
type: SequenceAnnotation
persistent identity: annotation2454130
display id: annotation2454130
version: 1
top level: BBa_K1789012
location: https://synbiohub.org/public/igem/BBa_K1789012/annotation2454130/range2454130/1
component: BBa_K1789002

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type: Range
persistent identity: range2454130
display id: range2454130
version: 1
direction: direction/reverse
top level: BBa_K1789012
start: 209
end: 223
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2454122
display id: annotation2454122
version: 1
top level: BBa_K1789012
location: https://synbiohub.org/public/igem/BBa_K1789012/annotation2454122/range2454122/1
component: BBa_R0010

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type: Range
persistent identity: range2454122
display id: range2454122
version: 1
direction: direction/forward
top level: BBa_K1789012
start: 1
end: 200
orientation: inline

BBa_K1499200
type: ComponentDefinition
persistent identity: BBa K1499200
display id: BBa_K1499200
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Deinococcus radiodurans UV DNA damage endonuclease - uvsE
creator: Raman Nelakanti
created: 2014-08-21T11:00:00Z
modified: 2015-05-08T01:10:46Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1879_
m user id: 0
owner id: 11095
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: TODO
mutable notes: TODO - mention the different starting points
mutable provenance: Deinococcus radiodurans R1 genomic DNA from Chromosome 1
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: TAA Stop
sequence annotation: ATG Start Codon
sequence annotation: uvsE
sequence: https://synbiohub.org/public/igem/BBa_K1499200_sequence/1

TAA Stop
type: SequenceAnnotation
persistent identity: annotation2381267
display id: annotation2381267
version: 1
top level: BBa_K1499200
role: SO:0000319
role: feature/stop
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type: Range
persistent identity: range2381267
display id: range2381267
version: 1
direction: direction/forward
top level: BBa_K1499200
start: 916
end: 918
orientation: inline

ATG Start Codon
type: SequenceAnnotation
persistent identity: annotation2381266
display id: annotation2381266
version: 1
top level: BBa_K1499200
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1499200/annotation2381266/range2381266/1

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type: Range
persistent identity: range2381266
display id: range2381266
version: 1
direction: direction/forward
top level: BBa_K1499200
start: 1
end: 3
orientation: inline

uvsE
type: SequenceAnnotation
persistent identity: annotation2427734
display id: annotation2427734
version: 1
top level: BBa_K1499200
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1499200/annotation2427734/range2427734/1

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type: Range
persistent identity: range2427734
display id: range2427734
version: 1
direction: direction/forward
top level: BBa_K1499200
start: 1
end: 918
orientation: inline

BBa_K1789011
type: ComponentDefinition
persistent identity: BBa K1789011
display id: BBa_K1789011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: IAAH+Ter
creator: Jiaqi Sun
created: 2015-09-13T11:00:00Z
modified: 2015-09-18T10:46:33Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2214_
m user id: 21134
owner id: 26298
owning group id: 9
status: status/Planning
bookmark: false
mutable description: It is the combination of IAAH and termination.
mutable notes: We combine IAAH and the termination to improve the efficiency of the building.
mutable provenance: It is constructed by ourselves.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_B0015
component: BBa_K1789001
sequence annotation: BBa_B0015
sequence annotation: BBa_K1789001
sequence: https://synbiohub.org/public/igem/BBa_K1789011_sequence/1

BBa_B0015
type: Component
persistent identity: component2454253
display id: component2454253
version: 1
top level: BBa_K1789011
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_K1789001
type: Component
persistent identity: component2454246
display id: component2454246
version: 1
top level: BBa_K1789011
access: public
definition: BBa_K1789001

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2454253
display id: annotation2454253
version: 1
top level: BBa_K1789011
location: https://synbiohub.org/public/igem/BBa_K1789011/annotation2454253/range2454253/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1789011/annotation2454253/range2454253/1
type: Range
persistent identity: range2454253
display id: range2454253
version: 1
direction: direction/reverse
top level: BBa_K1789011
start: 1344
end: 1472
orientation: inline

BBa_K1789001
type: SequenceAnnotation
persistent identity: annotation2454246
display id: annotation2454246
version: 1
top level: BBa_K1789011
location: https://synbiohub.org/public/igem/BBa_K1789011/annotation2454246/range2454246/1
component: BBa_K1789001

https://synbiohub.org/public/igem/BBa_K1789011/annotation2454246/range2454246/1
type: Range
persistent identity: range2454246
display id: range2454246
version: 1
direction: direction/reverse
top level: BBa_K1789011
start: 1
end: 1335
orientation: inline

BBa_K1632013
type: ComponentDefinition
persistent identity: BBa K1632013
display id: BBa_K1632013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PBAD/araC_rbs_fimE(wild-type)
creator: Riku Shinohara
created: 2015-08-29T11:00:00Z
modified: 2015-09-15T07:47:30Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2049_
m user id: 24760
owner id: 22687
owning group id: 9
status: status/Planning
bookmark: false
mutable description: a
mutable notes: a
mutable provenance: a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1632011
component: BBa_B0034
component: BBa_I0500
sequence annotation: BBa_I0500
sequence annotation: BBa_K1632011
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K1632013_sequence/1

BBa_K1632011
type: Component
persistent identity: component2440383
display id: component2440383
version: 1
top level: BBa_K1632013
access: public
definition: BBa_K1632011

BBa_B0034
type: Component
persistent identity: component2440382
display id: component2440382
version: 1
top level: BBa_K1632013
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_I0500
type: Component
persistent identity: component2440380
display id: component2440380
version: 1
top level: BBa_K1632013
access: public
definition: pBad/araC

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2440380
display id: annotation2440380
version: 1
top level: BBa_K1632013
location: https://synbiohub.org/public/igem/BBa_K1632013/annotation2440380/range2440380/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_K1632013/annotation2440380/range2440380/1
type: Range
persistent identity: range2440380
display id: range2440380
version: 1
direction: direction/reverse
top level: BBa_K1632013
start: 1
end: 1210
orientation: inline

BBa_K1632011
type: SequenceAnnotation
persistent identity: annotation2440383
display id: annotation2440383
version: 1
top level: BBa_K1632013
location: https://synbiohub.org/public/igem/BBa_K1632013/annotation2440383/range2440383/1
component: BBa_K1632011

https://synbiohub.org/public/igem/BBa_K1632013/annotation2440383/range2440383/1
type: Range
persistent identity: range2440383
display id: range2440383
version: 1
direction: direction/reverse
top level: BBa_K1632013
start: 1239
end: 1835
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2440382
display id: annotation2440382
version: 1
top level: BBa_K1632013
location: https://synbiohub.org/public/igem/BBa_K1632013/annotation2440382/range2440382/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1632013/annotation2440382/range2440382/1
type: Range
persistent identity: range2440382
display id: range2440382
version: 1
direction: direction/reverse
top level: BBa_K1632013
start: 1219
end: 1230
orientation: inline

BBa_K1467201
type: ComponentDefinition
persistent identity: BBa K1467201
display id: BBa_K1467201
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GoldenGate compatible AmilCP, Blue chromoprotein
creator: Cara Deal
created: 2014-09-23T11:00:00Z
modified: 2015-06-01T07:04:22Z
discontinued: false
dominant: false
group u list: _1846_
m user id: 4206
owner id: 21550
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: A variation of the part BBa_K592009 that has been made compatible with the GoldenGate MoClo Assembly Standard as it is free from BsaI and BpiI recognition sequences. The chromoprotein naturally exhibits strong blue color when expressed. Codon-optimised for expression in dicotyledenous plants.
mutable notes: Domesticated for GoldenGate by removal of internal BsaI and BpiI recognition sequences. Codon-optimised for expression in dicotyledenous plants.
mutable provenance: Acropora millepora
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K1467201_sequence/1

BBa_K1689005
type: ComponentDefinition
persistent identity: BBa K1689005
display id: BBa_K1689005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Coding sequence of FKBP-Cluc398
creator: Xiang Li
created: 2015-09-15T11:00:00Z
modified: 2015-09-18T04:19:09Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2107_
m user id: 26445
owner id: 26464
owning group id: 9
status: status/Planning
bookmark: false
mutable description: including firefly luciferase C terminal 398 amino acid domain, 2??linker, and FKBP.
mutable notes: FKBP and C terminal 398 amino acid domain are linked by 2??linker
mutable provenance: E.coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: start codon
sequence annotation: C398 terminal of firefly luciferase
sequence annotation: FKBP
sequence annotation: B0034
sequence annotation: J23110
sequence annotation: stop codon
sequence annotation: GGGSGGGGSG linker
sequence: https://synbiohub.org/public/igem/BBa_K1689005_sequence/1

start codon
type: SequenceAnnotation
persistent identity: annotation2470122
display id: annotation2470122
version: 1
top level: BBa_K1689005
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1689005/annotation2470122/range2470122/1

https://synbiohub.org/public/igem/BBa_K1689005/annotation2470122/range2470122/1
type: Range
persistent identity: range2470122
display id: range2470122
version: 1
direction: direction/forward
top level: BBa_K1689005
start: 48
end: 50
orientation: inline

C398 terminal of firefly luciferase
type: SequenceAnnotation
persistent identity: annotation2470137
display id: annotation2470137
version: 1
top level: BBa_K1689005
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1689005/annotation2470137/range2470137/1

https://synbiohub.org/public/igem/BBa_K1689005/annotation2470137/range2470137/1
type: Range
persistent identity: range2470137
display id: range2470137
version: 1
direction: direction/forward
top level: BBa_K1689005
start: 402
end: 860
orientation: inline

FKBP
type: SequenceAnnotation
persistent identity: annotation2470134
display id: annotation2470134
version: 1
top level: BBa_K1689005
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1689005/annotation2470134/range2470134/1

https://synbiohub.org/public/igem/BBa_K1689005/annotation2470134/range2470134/1
type: Range
persistent identity: range2470134
display id: range2470134
version: 1
direction: direction/forward
top level: BBa_K1689005
start: 51
end: 371
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2470121
display id: annotation2470121
version: 1
top level: BBa_K1689005
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1689005/annotation2470121/range2470121/1

https://synbiohub.org/public/igem/BBa_K1689005/annotation2470121/range2470121/1
type: Range
persistent identity: range2470121
display id: range2470121
version: 1
direction: direction/forward
top level: BBa_K1689005
start: 36
end: 47
orientation: inline

J23110
type: SequenceAnnotation
persistent identity: annotation2470118
display id: annotation2470118
version: 1
top level: BBa_K1689005
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1689005/annotation2470118/range2470118/1

https://synbiohub.org/public/igem/BBa_K1689005/annotation2470118/range2470118/1
type: Range
persistent identity: range2470118
display id: range2470118
version: 1
direction: direction/forward
top level: BBa_K1689005
start: 1
end: 35
orientation: inline

stop codon
type: SequenceAnnotation
persistent identity: annotation2470138
display id: annotation2470138
version: 1
top level: BBa_K1689005
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1689005/annotation2470138/range2470138/1

https://synbiohub.org/public/igem/BBa_K1689005/annotation2470138/range2470138/1
type: Range
persistent identity: range2470138
display id: range2470138
version: 1
direction: direction/forward
top level: BBa_K1689005
start: 861
end: 863
orientation: inline

GGGSGGGGSG linker
type: SequenceAnnotation
persistent identity: annotation2470136
display id: annotation2470136
version: 1
top level: BBa_K1689005
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1689005/annotation2470136/range2470136/1

https://synbiohub.org/public/igem/BBa_K1689005/annotation2470136/range2470136/1
type: Range
persistent identity: range2470136
display id: range2470136
version: 1
direction: direction/forward
top level: BBa_K1689005
start: 372
end: 401
orientation: inline

BBa_K1758361
type: ComponentDefinition
persistent identity: BBa K1758361
display id: BBa_K1758361
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: mRFP1 controlled by the GABA-inducible activator GabR
creator: Team Bielefeld-CeBiTec 2015
created: 2015-08-21T11:00:00Z
modified: 2015-09-18T02:48:20Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2180_
m user id: 26913
owner id: 26587
owning group id: 9
status: status/Planning
bookmark: true
mutable description: gabR encodes a repressor which controls the expression of mRFP1.
mutable notes: This device was created by 3A assembly and subsequent subcloning into pSB1C3.
mutable provenance: BBa_K1758350 was obtained by gene synthesis. BBa_K516030 was part of the distribution.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K516030
sequence annotation: BBa_K516030
sequence: https://synbiohub.org/public/igem/BBa_K1758361_sequence/1

BBa_K516030
type: Component
persistent identity: component2436864
display id: component2436864
version: 1
top level: BBa_K1758361
access: public
definition: https://synbiohub.org/public/igem/BBa_K516030/1

BBa_K516030
type: SequenceAnnotation
persistent identity: annotation2436864
display id: annotation2436864
version: 1
top level: BBa_K1758361
location: https://synbiohub.org/public/igem/BBa_K1758361/annotation2436864/range2436864/1
component: BBa_K516030

https://synbiohub.org/public/igem/BBa_K1758361/annotation2436864/range2436864/1
type: Range
persistent identity: range2436864
display id: range2436864
version: 1
direction: direction/reverse
top level: BBa_K1758361
start: 1555
end: 2418
orientation: inline

BBa_K1689001
type: ComponentDefinition
persistent identity: BBa K1689001
display id: BBa_K1689001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Coding sequence of STV-N-luc
creator: ZHANG Yihao
created: 2015-09-15T11:00:00Z
modified: 2016-01-19T02:50:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2107_
m user id: 4206
owner id: 26464
owning group id: 9
status: status/Planning
bookmark: false
mutable description: including streptavidin, 2??linker, and firefly luciferase N terminal 416 amino acid domain.
mutable notes: streptavidin and N terminal 416 amino acid domain was linked by 2??linker
mutable provenance: E.coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: linker
sequence annotation: random spacer
sequence annotation: start codon
sequence annotation: stop codon
sequence annotation: N416 terminal of firefly luciferase
sequence annotation: streptavidin
sequence: https://synbiohub.org/public/igem/BBa_K1689001_sequence/1

linker
type: SequenceAnnotation
persistent identity: annotation2469931
display id: annotation2469931
version: 1
top level: BBa_K1689001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1689001/annotation2469931/range2469931/1

https://synbiohub.org/public/igem/BBa_K1689001/annotation2469931/range2469931/1
type: Range
persistent identity: range2469931
display id: range2469931
version: 1
direction: direction/forward
top level: BBa_K1689001
start: 392
end: 421
orientation: inline

random spacer
type: SequenceAnnotation
persistent identity: annotation2477762
display id: annotation2477762
version: 1
top level: BBa_K1689001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1689001/annotation2477762/range2477762/1

https://synbiohub.org/public/igem/BBa_K1689001/annotation2477762/range2477762/1
type: Range
persistent identity: range2477762
display id: range2477762
version: 1
direction: direction/forward
top level: BBa_K1689001
start: 1673
end: 1727
orientation: inline

random spacer
type: SequenceAnnotation
persistent identity: annotation2477761
display id: annotation2477761
version: 1
top level: BBa_K1689001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1689001/annotation2477761/range2477761/1

https://synbiohub.org/public/igem/BBa_K1689001/annotation2477761/range2477761/1
type: Range
persistent identity: range2477761
display id: range2477761
version: 1
direction: direction/forward
top level: BBa_K1689001
start: 1
end: 34
orientation: inline

start codon
type: SequenceAnnotation
persistent identity: annotation2469928
display id: annotation2469928
version: 1
top level: BBa_K1689001
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1689001/annotation2469928/range2469928/1

https://synbiohub.org/public/igem/BBa_K1689001/annotation2469928/range2469928/1
type: Range
persistent identity: range2469928
display id: range2469928
version: 1
direction: direction/forward
top level: BBa_K1689001
start: 35
end: 37
orientation: inline

stop codon
type: SequenceAnnotation
persistent identity: annotation2469934
display id: annotation2469934
version: 1
top level: BBa_K1689001
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1689001/annotation2469934/range2469934/1

https://synbiohub.org/public/igem/BBa_K1689001/annotation2469934/range2469934/1
type: Range
persistent identity: range2469934
display id: range2469934
version: 1
direction: direction/forward
top level: BBa_K1689001
start: 1670
end: 1672
orientation: inline

N416 terminal of firefly luciferase
type: SequenceAnnotation
persistent identity: annotation2469932
display id: annotation2469932
version: 1
top level: BBa_K1689001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1689001/annotation2469932/range2469932/1

https://synbiohub.org/public/igem/BBa_K1689001/annotation2469932/range2469932/1
type: Range
persistent identity: range2469932
display id: range2469932
version: 1
direction: direction/forward
top level: BBa_K1689001
start: 422
end: 1669
orientation: inline

streptavidin
type: SequenceAnnotation
persistent identity: annotation2469930
display id: annotation2469930
version: 1
top level: BBa_K1689001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1689001/annotation2469930/range2469930/1

https://synbiohub.org/public/igem/BBa_K1689001/annotation2469930/range2469930/1
type: Range
persistent identity: range2469930
display id: range2469930
version: 1
direction: direction/forward
top level: BBa_K1689001
start: 38
end: 391
orientation: inline

tFba1
type: ComponentDefinition
persistent identity: BBa K1829004
display id: BBa_K1829004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: tFba1: yeast terminator
creator: Nicholas Suen, Hunter Collins
created: 2015-09-09T11:00:00Z
modified: 2015-09-18T06:14:38Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2255_
m user id: 27847
owner id: 27842
owning group id: 9
sample status: No part sequence
status: status/Planning
bookmark: false
mutable description: tENO2 is a terminator
mutable notes: cats
mutable provenance: cats
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence: https://synbiohub.org/public/igem/BBa_K1829004_sequence/1

BBa_K1460002
type: ComponentDefinition
persistent identity: BBa K1460002
display id: BBa_K1460002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS + GST (glutathione-S-transferase)-CRS5 (metallothionein) + Ter
creator: Eric Holmes
created: 2014-08-13T11:00:00Z
modified: 2016-01-13T10:26:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1839_
m user id: 4206
owner id: 15649
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: his part serves as a functional metallothionein for heavy metal binding and conferred resistance to heavy metals when combined with an appropriate promoter. CRS5 gene is the metallothionein that binds to heavy metals and it is fused to GST for stability.
mutable notes: The part contains a KpnI cut site between the biobrick prefix and the RBS that is a result of removing the T7 promoter from part BBa_K1460001.
mutable provenance: This part is BBa_K1460001 with the T7 promoter removed.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: BBa_J61101
sequence annotation: Linker Sequence
sequence annotation: CRS5
sequence annotation: GST
sequence annotation: BBa_B1006
sequence: https://synbiohub.org/public/igem/BBa_K1460002_sequence/1

BBa_J61101
type: SequenceAnnotation
persistent identity: annotation2380987
display id: annotation2380987
version: 1
top level: BBa_K1460002
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1460002/annotation2380987/range2380987/1

https://synbiohub.org/public/igem/BBa_K1460002/annotation2380987/range2380987/1
type: Range
persistent identity: range2380987
display id: range2380987
version: 1
direction: direction/forward
top level: BBa_K1460002
start: 14
end: 27
orientation: inline

Linker Sequence
type: SequenceAnnotation
persistent identity: annotation2380989
display id: annotation2380989
version: 1
top level: BBa_K1460002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1460002/annotation2380989/range2380989/1

https://synbiohub.org/public/igem/BBa_K1460002/annotation2380989/range2380989/1
type: Range
persistent identity: range2380989
display id: range2380989
version: 1
direction: direction/forward
top level: BBa_K1460002
start: 688
end: 720
orientation: inline

CRS5
type: SequenceAnnotation
persistent identity: annotation2380990
display id: annotation2380990
version: 1
top level: BBa_K1460002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1460002/annotation2380990/range2380990/1

https://synbiohub.org/public/igem/BBa_K1460002/annotation2380990/range2380990/1
type: Range
persistent identity: range2380990
display id: range2380990
version: 1
direction: direction/forward
top level: BBa_K1460002
start: 721
end: 930
orientation: inline

GST
type: SequenceAnnotation
persistent identity: annotation2380988
display id: annotation2380988
version: 1
top level: BBa_K1460002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1460002/annotation2380988/range2380988/1

https://synbiohub.org/public/igem/BBa_K1460002/annotation2380988/range2380988/1
type: Range
persistent identity: range2380988
display id: range2380988
version: 1
direction: direction/forward
top level: BBa_K1460002
start: 34
end: 687
orientation: inline

BBa_B1006
type: SequenceAnnotation
persistent identity: annotation2380991
display id: annotation2380991
version: 1
top level: BBa_K1460002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1460002/annotation2380991/range2380991/1

https://synbiohub.org/public/igem/BBa_K1460002/annotation2380991/range2380991/1
type: Range
persistent identity: range2380991
display id: range2380991
version: 1
direction: direction/forward
top level: BBa_K1460002
start: 931
end: 969
orientation: inline

BBa_K1632018
type: ComponentDefinition
persistent identity: BBa K1632018
display id: BBa_K1632018
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23100_rbs_lasR_TT_Plux_rbs_fimE(wild-type)
creator: Jun kawamura
created: 2015-08-29T11:00:00Z
modified: 2015-09-15T07:54:26Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2049_
m user id: 24760
owner id: 24760
owning group id: 9
status: status/Planning
bookmark: false
mutable description: aaaaaaaa
mutable notes: a
mutable provenance: aa
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_R0062
component: BBa_K553003
component: BBa_B0034
component: BBa_K1632011
sequence annotation: BBa_K553003
sequence annotation: BBa_K1632011
sequence annotation: BBa_B0034
sequence annotation: BBa_R0062
sequence: https://synbiohub.org/public/igem/BBa_K1632018_sequence/1

BBa_R0062
type: Component
persistent identity: component2440278
display id: component2440278
version: 1
top level: BBa_K1632018
access: public
definition: https://synbiohub.org/public/igem/BBa_R0062/1

BBa_K553003
type: Component
persistent identity: component2440276
display id: component2440276
version: 1
top level: BBa_K1632018
access: public
definition: https://synbiohub.org/public/igem/BBa_K553003/1

BBa_B0034
type: Component
persistent identity: component2440283
display id: component2440283
version: 1
top level: BBa_K1632018
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1632011
type: Component
persistent identity: component2440284
display id: component2440284
version: 1
top level: BBa_K1632018
access: public
definition: BBa_K1632011

BBa_K553003
type: SequenceAnnotation
persistent identity: annotation2440276
display id: annotation2440276
version: 1
top level: BBa_K1632018
location: https://synbiohub.org/public/igem/BBa_K1632018/annotation2440276/range2440276/1
component: BBa_K553003

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type: Range
persistent identity: range2440276
display id: range2440276
version: 1
direction: direction/reverse
top level: BBa_K1632018
start: 1
end: 921
orientation: inline

BBa_K1632011
type: SequenceAnnotation
persistent identity: annotation2440284
display id: annotation2440284
version: 1
top level: BBa_K1632018
location: https://synbiohub.org/public/igem/BBa_K1632018/annotation2440284/range2440284/1
component: BBa_K1632011

https://synbiohub.org/public/igem/BBa_K1632018/annotation2440284/range2440284/1
type: Range
persistent identity: range2440284
display id: range2440284
version: 1
direction: direction/reverse
top level: BBa_K1632018
start: 1013
end: 1609
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2440283
display id: annotation2440283
version: 1
top level: BBa_K1632018
location: https://synbiohub.org/public/igem/BBa_K1632018/annotation2440283/range2440283/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1632018/annotation2440283/range2440283/1
type: Range
persistent identity: range2440283
display id: range2440283
version: 1
direction: direction/reverse
top level: BBa_K1632018
start: 993
end: 1004
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation2440278
display id: annotation2440278
version: 1
top level: BBa_K1632018
location: https://synbiohub.org/public/igem/BBa_K1632018/annotation2440278/range2440278/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_K1632018/annotation2440278/range2440278/1
type: Range
persistent identity: range2440278
display id: range2440278
version: 1
direction: direction/forward
top level: BBa_K1632018
start: 930
end: 984
orientation: inline

BBa_K1321152
type: ComponentDefinition
persistent identity: BBa K1321152
display id: BBa_K1321152
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: metallothionein Fmt fused to CBDcex
creator: Gabriella Santosa
created: 2014-10-08T11:00:00Z
modified: 2015-06-17T12:23:40Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 4206
owner id: 20961
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: GATC
mutable notes: GATC
mutable provenance: GATC
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1321012
component: BBa_B0105
component: BBa_K1321003
sequence annotation: BBa_K1321012
sequence annotation: BBa_K1321003
sequence annotation: BBa_B0105
sequence: https://synbiohub.org/public/igem/BBa_K1321152_sequence/1

BBa_K1321012
type: Component
persistent identity: component2415037
display id: component2415037
version: 1
top level: BBa_K1321152
access: public
definition: https://synbiohub.org/public/igem/BBa_K1321012/1

BBa_B0105
type: Component
persistent identity: component2415039
display id: component2415039
version: 1
top level: BBa_K1321152
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_K1321003
type: Component
persistent identity: component2415040
display id: component2415040
version: 1
top level: BBa_K1321152
access: public
definition: https://synbiohub.org/public/igem/BBa_K1321003/1

BBa_K1321012
type: SequenceAnnotation
persistent identity: annotation2415037
display id: annotation2415037
version: 1
top level: BBa_K1321152
location: https://synbiohub.org/public/igem/BBa_K1321152/annotation2415037/range2415037/1
component: BBa_K1321012

https://synbiohub.org/public/igem/BBa_K1321152/annotation2415037/range2415037/1
type: Range
persistent identity: range2415037
display id: range2415037
version: 1
direction: direction/reverse
top level: BBa_K1321152
start: 1
end: 198
orientation: inline

BBa_K1321003
type: SequenceAnnotation
persistent identity: annotation2415040
display id: annotation2415040
version: 1
top level: BBa_K1321152
location: https://synbiohub.org/public/igem/BBa_K1321152/annotation2415040/range2415040/1
component: BBa_K1321003

https://synbiohub.org/public/igem/BBa_K1321152/annotation2415040/range2415040/1
type: Range
persistent identity: range2415040
display id: range2415040
version: 1
direction: direction/reverse
top level: BBa_K1321152
start: 205
end: 531
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2415039
display id: annotation2415039
version: 1
top level: BBa_K1321152
location: https://synbiohub.org/public/igem/BBa_K1321152/annotation2415039/range2415039/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_K1321152/annotation2415039/range2415039/1
type: Range
persistent identity: range2415039
display id: range2415039
version: 1
direction: direction/reverse
top level: BBa_K1321152
start: 199
end: 204
orientation: inline

aGal
type: ComponentDefinition
persistent identity: BBa K1483002
display id: BBa_K1483002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: α-Galactosidase
creator: Philipp H. O. Mayer and Philip Roessler
created: 2014-10-05T11:00:00Z
modified: 2015-05-08T01:10:41Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1863_
m user id: 0
owner id: 21259
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Encodes for an enzyme, that is capable of cleaving off galactose from B-group blood antigens, thereby turning them into H-antigens. Can be used to convert erythrocytes from blood type A to O. Part in RFC25 standard.
mutable notes: Part in RFC25, in order to ease fusion with other parts, for example immobilisation tags.
mutable provenance: The protein encoded by this sequence naturally occurs in Bacteroides fragilis. This sequence was optimized for codon usage in E. coli K12 and synthesized.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: alpha-Galactosidase
sequence: https://synbiohub.org/public/igem/BBa_K1483002_sequence/1

alpha-Galactosidase
type: SequenceAnnotation
persistent identity: annotation2396465
display id: annotation2396465
version: 1
top level: aGal
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1483002/annotation2396465/range2396465/1

https://synbiohub.org/public/igem/BBa_K1483002/annotation2396465/range2396465/1
type: Range
persistent identity: range2396465
display id: range2396465
version: 1
direction: direction/forward
top level: aGal
start: 1
end: 1752
orientation: inline

BBa_K1825005
type: ComponentDefinition
persistent identity: BBa K1825005
display id: BBa_K1825005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: nptII
creator: Jonathan Asmund Arnesen
created: 2015-09-02T11:00:00Z
modified: 2015-12-16T12:17:11Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2251_
m user id: 4206
owner id: 28047
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Neomycin Phosphotransferase, for a composite biobrick.
mutable notes: none
mutable provenance: From a vector.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1825005_sequence/1

BBa_K1692005
type: ComponentDefinition
persistent identity: BBa K1692005
display id: BBa_K1692005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: UbiX
creator: Daniel Kunin
created: 2015-08-02T11:00:00Z
modified: 2015-08-03T12:24:31Z
discontinued: false
dominant: false
group u list: _2110_
m user id: 26209
owner id: 26209
owning group id: 9
status: status/Planning
bookmark: false
mutable description: UbiX is a flavin prenyltransferase required in ubiquinone synthesis in Escherichia coli.
mutable notes: No considerations
mutable provenance: Escherichia coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1692005_sequence/1

BBa_K1699002
type: ComponentDefinition
persistent identity: BBa K1699002
display id: BBa_K1699002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: gRNA for SaCas9 targeting human ubiquitin B gene
creator: Emil Ruvinov
created: 2015-08-08T11:00:00Z
modified: 2015-09-13T06:26:57Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2117_
m user id: 24826
owner id: 24826
owning group id: 9
status: status/Planning
bookmark: false
mutable description: gRNA for SaCas9 targeting ubiquitin B
mutable notes: design
mutable provenance: source
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: HDV ribozyme
sequence annotation: Hammerhead ribozyme
sequence annotation: gRNA scaffold for SaCas9
sequence annotation: gRNA-UBB-Exon 2
sequence: https://synbiohub.org/public/igem/BBa_K1699002_sequence/1

HDV ribozyme
type: SequenceAnnotation
persistent identity: annotation2434598
display id: annotation2434598
version: 1
top level: BBa_K1699002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1699002/annotation2434598/range2434598/1

https://synbiohub.org/public/igem/BBa_K1699002/annotation2434598/range2434598/1
type: Range
persistent identity: range2434598
display id: range2434598
version: 1
direction: direction/forward
top level: BBa_K1699002
start: 147
end: 212
orientation: inline

Hammerhead ribozyme
type: SequenceAnnotation
persistent identity: annotation2434595
display id: annotation2434595
version: 1
top level: BBa_K1699002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1699002/annotation2434595/range2434595/1

https://synbiohub.org/public/igem/BBa_K1699002/annotation2434595/range2434595/1
type: Range
persistent identity: range2434595
display id: range2434595
version: 1
direction: direction/forward
top level: BBa_K1699002
start: 1
end: 43
orientation: inline

gRNA scaffold for SaCas9
type: SequenceAnnotation
persistent identity: annotation2434597
display id: annotation2434597
version: 1
top level: BBa_K1699002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1699002/annotation2434597/range2434597/1

https://synbiohub.org/public/igem/BBa_K1699002/annotation2434597/range2434597/1
type: Range
persistent identity: range2434597
display id: range2434597
version: 1
direction: direction/forward
top level: BBa_K1699002
start: 66
end: 146
orientation: inline

gRNA-UBB-Exon 2
type: SequenceAnnotation
persistent identity: annotation2434596
display id: annotation2434596
version: 1
top level: BBa_K1699002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1699002/annotation2434596/range2434596/1

https://synbiohub.org/public/igem/BBa_K1699002/annotation2434596/range2434596/1
type: Range
persistent identity: range2434596
display id: range2434596
version: 1
direction: direction/forward
top level: BBa_K1699002
start: 44
end: 65
orientation: inline

BBa_K1399008
type: ComponentDefinition
persistent identity: BBa K1399008
display id: BBa_K1399008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP (mut3b) with DAS-ssrA degradation tag
creator: Anna Stikane
created: 2014-09-18T11:00:00Z
modified: 2015-05-08T01:10:16Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1777_
m user id: 0
owner id: 22477
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: GFP (mut3b) (see part BBa_E0040) with added DAS-ssrA degradation tag (see part BBa_M0052). The tag increases GFP turn-over rate, thus providing better temporal resolution of green fluorescence. In the same time, maximal fluorescence amplitudes will be lower as newly formed protein is degraded as soon as it is formed. SsrA tags encode peptide sequence that is recognized by ClpA and ClpX unfoldases and ClpX mediator SspB.[1] ClpA and ClpX then form a proteosome-like complex with ClpP protease and the protein is degraded.[1] The final three residues of the tag determines the strength of interaction with ClpX and thus the final protein degradation rate.[2] The DAS tag encodes peptide sequence AANDENYADAS is reported to have low affinity to ClpX thus its mediated degradation very much depends on the concentration of SspB (ClpX mediator).[1] However, be aware that exact protein degradation rate is influenced by multiple other factors: ClpXP and ClpAP protease concentrations, protein stability, Km of binding to the protease, temperature [3]. ===References=== [1] Flynn, J. M. et al. Overlapping recognition determinants within the ssrA degradation tag allow modulation of proteolysis. Proc. Natl. Acad. Sci. U. S. A. 98, 10584???9 (2001). [2] Andersen, J. B. et al. New unstable variants of green fluorescent protein for studies of transient gene expression in bacteria. Appl. Environ. Microbiol. 64, 2240???6 (1998). [3] Purcell, O., Grierson, C. S., Bernardo, M. Di & Savery, N. J. Temperature dependence of ssrA-tag mediated protein degradation. J. Biol. Eng. 6, 10 (2012).
mutable notes: The tag was attached to GFP using PCR and MABEL (mutagenesis with blunt-end ligation), thus avoiding introduction of additional residues and restriction site. Different parts of the tag are recognized by different proteins, for example, the final 3 residues (DAS in this case) are recognized by ClpX, whereas first 4 residues of the tag are required for efficient SspB binding.[1] Thus modifications of these critical residues alter the efficacy with what different proteases bind to it.
mutable provenance: GFP comes from part BBa_E0040, the tag sequence was obtained from part BBa_M0052.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: stop
sequence annotation: GFP (mut3b)
sequence annotation: DAS-ssrA tag
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_K1399008_sequence/1

stop
type: SequenceAnnotation
persistent identity: annotation2383932
display id: annotation2383932
version: 1
top level: BBa_K1399008
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399008/annotation2383932/range2383932/1

https://synbiohub.org/public/igem/BBa_K1399008/annotation2383932/range2383932/1
type: Range
persistent identity: range2383932
display id: range2383932
version: 1
direction: direction/forward
top level: BBa_K1399008
start: 751
end: 753
orientation: inline

GFP (mut3b)
type: SequenceAnnotation
persistent identity: annotation2383929
display id: annotation2383929
version: 1
top level: BBa_K1399008
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1399008/annotation2383929/range2383929/1

https://synbiohub.org/public/igem/BBa_K1399008/annotation2383929/range2383929/1
type: Range
persistent identity: range2383929
display id: range2383929
version: 1
direction: direction/forward
top level: BBa_K1399008
start: 3
end: 714
orientation: inline

DAS-ssrA tag
type: SequenceAnnotation
persistent identity: annotation2383930
display id: annotation2383930
version: 1
top level: BBa_K1399008
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1399008/annotation2383930/range2383930/1

https://synbiohub.org/public/igem/BBa_K1399008/annotation2383930/range2383930/1
type: Range
persistent identity: range2383930
display id: range2383930
version: 1
direction: direction/forward
top level: BBa_K1399008
start: 715
end: 747
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2383931
display id: annotation2383931
version: 1
top level: BBa_K1399008
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399008/annotation2383931/range2383931/1

https://synbiohub.org/public/igem/BBa_K1399008/annotation2383931/range2383931/1
type: Range
persistent identity: range2383931
display id: range2383931
version: 1
direction: direction/forward
top level: BBa_K1399008
start: 748
end: 750
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2383928
display id: annotation2383928
version: 1
top level: BBa_K1399008
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1399008/annotation2383928/range2383928/1

https://synbiohub.org/public/igem/BBa_K1399008/annotation2383928/range2383928/1
type: Range
persistent identity: range2383928
display id: range2383928
version: 1
direction: direction/forward
top level: BBa_K1399008
start: 1
end: 3
orientation: inline

BBa_K1499202
type: ComponentDefinition
persistent identity: BBa K1499202
display id: BBa_K1499202
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Deinococcus radiodurans uracil-DNA glycosylase 2
creator: Raman Nelakanti
created: 2014-08-24T11:00:00Z
modified: 2015-05-08T01:10:46Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1879_
m user id: 0
owner id: 11095
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: todo
mutable notes: todo
mutable provenance: todo
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Start Codon
sequence annotation: uracil-DNA glycosylase 2
sequence annotation: TAA Stop
sequence: https://synbiohub.org/public/igem/BBa_K1499202_sequence/1

Start Codon
type: SequenceAnnotation
persistent identity: annotation2381352
display id: annotation2381352
version: 1
top level: BBa_K1499202
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1499202/annotation2381352/range2381352/1

https://synbiohub.org/public/igem/BBa_K1499202/annotation2381352/range2381352/1
type: Range
persistent identity: range2381352
display id: range2381352
version: 1
direction: direction/forward
top level: BBa_K1499202
start: 1
end: 3
orientation: inline

uracil-DNA glycosylase 2
type: SequenceAnnotation
persistent identity: annotation2429042
display id: annotation2429042
version: 1
top level: BBa_K1499202
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1499202/annotation2429042/range2429042/1

https://synbiohub.org/public/igem/BBa_K1499202/annotation2429042/range2429042/1
type: Range
persistent identity: range2429042
display id: range2429042
version: 1
direction: direction/forward
top level: BBa_K1499202
start: 1
end: 714
orientation: inline

TAA Stop
type: SequenceAnnotation
persistent identity: annotation2381353
display id: annotation2381353
version: 1
top level: BBa_K1499202
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1499202/annotation2381353/range2381353/1

https://synbiohub.org/public/igem/BBa_K1499202/annotation2381353/range2381353/1
type: Range
persistent identity: range2381353
display id: range2381353
version: 1
direction: direction/forward
top level: BBa_K1499202
start: 712
end: 714
orientation: inline

SspDnaB(C)
type: ComponentDefinition
persistent identity: BBa K1362141
display id: BBa_K1362141
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS + SspDnaB C-intein RFC[105] assembly construct (with His6)
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B??scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch??fer, Carolin Schmelas, Silvan Schmitz, Max Waldha
created: 2014-10-08T11:00:00Z
modified: 2015-05-08T01:10:05Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1738_
m user id: 0
owner id: 12377
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: ???
mutable notes: ???
mutable provenance: ???
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_G0000
component: BBa_K1362418
component: BBa_K1362431
component: BBa_K1362419
component: BBa_K1362422
component: BBa_K1362465
component: BBa_K1362414
component: BBa_K1362421
component: BBa_K1362090
component: BBa_K1362467
component: BBa_K1362409
sequence annotation: BBa_K1362409
sequence annotation: BBa_K1362467
sequence annotation: BBa_K1362414
sequence annotation: BBa_G0000
sequence annotation: BBa_K1362418
sequence annotation: BBa_K1362465
sequence annotation: BBa_K1362090
sequence annotation: BBa_K1362419
sequence annotation: BBa_K1362422
sequence annotation: BBa_K1362431
sequence annotation: BBa_K1362421
sequence: https://synbiohub.org/public/igem/BBa_K1362141_sequence/1

BBa_G0000
type: Component
persistent identity: component2430315
display id: component2430315
version: 1
top level: SspDnaB(C)
access: public
definition: https://synbiohub.org/public/igem/BBa_G0000/1

BBa_K1362418
type: Component
persistent identity: component2430320
display id: component2430320
version: 1
top level: SspDnaB(C)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362418/1

BBa_K1362431
type: Component
persistent identity: component2430319
display id: component2430319
version: 1
top level: SspDnaB(C)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362431/1

BBa_K1362419
type: Component
persistent identity: component2430323
display id: component2430323
version: 1
top level: SspDnaB(C)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362419/1

BBa_K1362422
type: Component
persistent identity: component2430344
display id: component2430344
version: 1
top level: SspDnaB(C)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362422/1

BBa_K1362465
type: Component
persistent identity: component2430347
display id: component2430347
version: 1
top level: SspDnaB(C)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362465/1

BBa_K1362414
type: Component
persistent identity: component2430316
display id: component2430316
version: 1
top level: SspDnaB(C)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362414/1

BBa_K1362421
type: Component
persistent identity: component2430346
display id: component2430346
version: 1
top level: SspDnaB(C)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362421/1

BBa_K1362090
type: Component
persistent identity: component2430314
display id: component2430314
version: 1
top level: SspDnaB(C)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362090/1

BBa_K1362467
type: Component
persistent identity: component2430321
display id: component2430321
version: 1
top level: SspDnaB(C)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362467/1

BBa_K1362409
type: Component
persistent identity: component2430322
display id: component2430322
version: 1
top level: SspDnaB(C)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362409/1

BBa_K1362409
type: SequenceAnnotation
persistent identity: annotation2430322
display id: annotation2430322
version: 1
top level: SspDnaB(C)
location: https://synbiohub.org/public/igem/BBa_K1362141/annotation2430322/range2430322/1
component: BBa_K1362409

https://synbiohub.org/public/igem/BBa_K1362141/annotation2430322/range2430322/1
type: Range
persistent identity: range2430322
display id: range2430322
version: 1
direction: direction/reverse
top level: SspDnaB(C)
start: 66
end: 490
orientation: inline

BBa_K1362467
type: SequenceAnnotation
persistent identity: annotation2430321
display id: annotation2430321
version: 1
top level: SspDnaB(C)
location: https://synbiohub.org/public/igem/BBa_K1362141/annotation2430321/range2430321/1
component: BBa_K1362467

https://synbiohub.org/public/igem/BBa_K1362141/annotation2430321/range2430321/1
type: Range
persistent identity: range2430321
display id: range2430321
version: 1
direction: direction/reverse
top level: SspDnaB(C)
start: 64
end: 65
orientation: inline

BBa_K1362414
type: SequenceAnnotation
persistent identity: annotation2430316
display id: annotation2430316
version: 1
top level: SspDnaB(C)
location: https://synbiohub.org/public/igem/BBa_K1362141/annotation2430316/range2430316/1
component: BBa_K1362414

https://synbiohub.org/public/igem/BBa_K1362141/annotation2430316/range2430316/1
type: Range
persistent identity: range2430316
display id: range2430316
version: 1
direction: direction/reverse
top level: SspDnaB(C)
start: 36
end: 38
orientation: inline

BBa_G0000
type: SequenceAnnotation
persistent identity: annotation2430315
display id: annotation2430315
version: 1
top level: SspDnaB(C)
location: https://synbiohub.org/public/igem/BBa_K1362141/annotation2430315/range2430315/1
component: BBa_G0000

https://synbiohub.org/public/igem/BBa_K1362141/annotation2430315/range2430315/1
type: Range
persistent identity: range2430315
display id: range2430315
version: 1
direction: direction/reverse
top level: SspDnaB(C)
start: 30
end: 35
orientation: inline

BBa_K1362418
type: SequenceAnnotation
persistent identity: annotation2430320
display id: annotation2430320
version: 1
top level: SspDnaB(C)
location: https://synbiohub.org/public/igem/BBa_K1362141/annotation2430320/range2430320/1
component: BBa_K1362418

https://synbiohub.org/public/igem/BBa_K1362141/annotation2430320/range2430320/1
type: Range
persistent identity: range2430320
display id: range2430320
version: 1
direction: direction/reverse
top level: SspDnaB(C)
start: 60
end: 63
orientation: inline

BBa_K1362465
type: SequenceAnnotation
persistent identity: annotation2430347
display id: annotation2430347
version: 1
top level: SspDnaB(C)
location: https://synbiohub.org/public/igem/BBa_K1362141/annotation2430347/range2430347/1
component: BBa_K1362465

https://synbiohub.org/public/igem/BBa_K1362141/annotation2430347/range2430347/1
type: Range
persistent identity: range2430347
display id: range2430347
version: 1
direction: direction/reverse
top level: SspDnaB(C)
start: 1582
end: 1583
orientation: inline

BBa_K1362090
type: SequenceAnnotation
persistent identity: annotation2430314
display id: annotation2430314
version: 1
top level: SspDnaB(C)
location: https://synbiohub.org/public/igem/BBa_K1362141/annotation2430314/range2430314/1
component: BBa_K1362090

https://synbiohub.org/public/igem/BBa_K1362141/annotation2430314/range2430314/1
type: Range
persistent identity: range2430314
display id: range2430314
version: 1
direction: direction/reverse
top level: SspDnaB(C)
start: 1
end: 29
orientation: inline

BBa_K1362419
type: SequenceAnnotation
persistent identity: annotation2430323
display id: annotation2430323
version: 1
top level: SspDnaB(C)
location: https://synbiohub.org/public/igem/BBa_K1362141/annotation2430323/range2430323/1
component: BBa_K1362419

https://synbiohub.org/public/igem/BBa_K1362141/annotation2430323/range2430323/1
type: Range
persistent identity: range2430323
display id: range2430323
version: 1
direction: direction/reverse
top level: SspDnaB(C)
start: 491
end: 494
orientation: inline

BBa_K1362422
type: SequenceAnnotation
persistent identity: annotation2430344
display id: annotation2430344
version: 1
top level: SspDnaB(C)
location: https://synbiohub.org/public/igem/BBa_K1362141/annotation2430344/range2430344/1
component: BBa_K1362422

https://synbiohub.org/public/igem/BBa_K1362141/annotation2430344/range2430344/1
type: Range
persistent identity: range2430344
display id: range2430344
version: 1
direction: direction/reverse
top level: SspDnaB(C)
start: 495
end: 1577
orientation: inline

BBa_K1362431
type: SequenceAnnotation
persistent identity: annotation2430319
display id: annotation2430319
version: 1
top level: SspDnaB(C)
location: https://synbiohub.org/public/igem/BBa_K1362141/annotation2430319/range2430319/1
component: BBa_K1362431

https://synbiohub.org/public/igem/BBa_K1362141/annotation2430319/range2430319/1
type: Range
persistent identity: range2430319
display id: range2430319
version: 1
direction: direction/reverse
top level: SspDnaB(C)
start: 39
end: 59
orientation: inline

BBa_K1362421
type: SequenceAnnotation
persistent identity: annotation2430346
display id: annotation2430346
version: 1
top level: SspDnaB(C)
location: https://synbiohub.org/public/igem/BBa_K1362141/annotation2430346/range2430346/1
component: BBa_K1362421

https://synbiohub.org/public/igem/BBa_K1362141/annotation2430346/range2430346/1
type: Range
persistent identity: range2430346
display id: range2430346
version: 1
direction: direction/reverse
top level: SspDnaB(C)
start: 1578
end: 1581
orientation: inline

BBa_K1321125
type: ComponentDefinition
persistent identity: BBa K1321125
display id: BBa_K1321125
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CBD7+Smt
creator: Gabriella Santosa
created: 2014-10-08T11:00:00Z
modified: 2015-05-08T01:09:52Z
discontinued: true
dominant: false
experience: experience/None
group u list: _1696_
m user id: 0
owner id: 20961
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: GATC
mutable notes: GATC
mutable provenance: GATC
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1321003
component: BBa_B0105
component: BBa_K1321011
sequence annotation: BBa_B0105
sequence annotation: BBa_K1321003
sequence annotation: BBa_K1321011
sequence: https://synbiohub.org/public/igem/BBa_K1321125_sequence/1

BBa_K1321003
type: Component
persistent identity: component2414712
display id: component2414712
version: 1
top level: BBa_K1321125
access: public
definition: https://synbiohub.org/public/igem/BBa_K1321003/1

BBa_B0105
type: Component
persistent identity: component2414714
display id: component2414714
version: 1
top level: BBa_K1321125
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_K1321011
type: Component
persistent identity: component2414716
display id: component2414716
version: 1
top level: BBa_K1321125
access: public
definition: https://synbiohub.org/public/igem/BBa_K1321011/1

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2414714
display id: annotation2414714
version: 1
top level: BBa_K1321125
location: https://synbiohub.org/public/igem/BBa_K1321125/annotation2414714/range2414714/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_K1321125/annotation2414714/range2414714/1
type: Range
persistent identity: range2414714
display id: range2414714
version: 1
direction: direction/reverse
top level: BBa_K1321125
start: 328
end: 333
orientation: inline

BBa_K1321003
type: SequenceAnnotation
persistent identity: annotation2414712
display id: annotation2414712
version: 1
top level: BBa_K1321125
location: https://synbiohub.org/public/igem/BBa_K1321125/annotation2414712/range2414712/1
component: BBa_K1321003

https://synbiohub.org/public/igem/BBa_K1321125/annotation2414712/range2414712/1
type: Range
persistent identity: range2414712
display id: range2414712
version: 1
direction: direction/reverse
top level: BBa_K1321125
start: 1
end: 327
orientation: inline

BBa_K1321011
type: SequenceAnnotation
persistent identity: annotation2414716
display id: annotation2414716
version: 1
top level: BBa_K1321125
location: https://synbiohub.org/public/igem/BBa_K1321125/annotation2414716/range2414716/1
component: BBa_K1321011

https://synbiohub.org/public/igem/BBa_K1321125/annotation2414716/range2414716/1
type: Range
persistent identity: range2414716
display id: range2414716
version: 1
direction: direction/reverse
top level: BBa_K1321125
start: 334
end: 498
orientation: inline

BBa_K1758321
type: ComponentDefinition
persistent identity: BBa K1758321
display id: BBa_K1758321
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: mRFP under control of copper responsive promoter
creator: Team Bielefeld-CeBiTec 2015
created: 2015-08-28T11:00:00Z
modified: 2016-01-06T03:42:39Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2180_
m user id: 4206
owner id: 26630
owning group id: 9
status: status/Planning
bookmark: false
mutable description: A copper iduceble promoter with RFP for detection
mutable notes: ---
mutable provenance: CopAp is a copper induceble promoter out of ``E.coli`` K12
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: CopAP
sequence annotation: mRFP
sequence: https://synbiohub.org/public/igem/BBa_K1758321_sequence/1

CopAP
type: SequenceAnnotation
persistent identity: annotation2440066
display id: annotation2440066
version: 1
top level: BBa_K1758321
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1758321/annotation2440066/range2440066/1

https://synbiohub.org/public/igem/BBa_K1758321/annotation2440066/range2440066/1
type: Range
persistent identity: range2440066
display id: range2440066
version: 1
direction: direction/forward
top level: BBa_K1758321
start: 1
end: 83
orientation: inline

mRFP
type: SequenceAnnotation
persistent identity: annotation2440067
display id: annotation2440067
version: 1
top level: BBa_K1758321
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1758321/annotation2440067/range2440067/1

https://synbiohub.org/public/igem/BBa_K1758321/annotation2440067/range2440067/1
type: Range
persistent identity: range2440067
display id: range2440067
version: 1
direction: direction/forward
top level: BBa_K1758321
start: 90
end: 770
orientation: inline

BBa_K1424003
type: ComponentDefinition
persistent identity: BBa K1424003
display id: BBa_K1424003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Kanamycin resistance for Synechocystis
creator: Jacob Lamb
created: 2014-09-24T11:00:00Z
modified: 2015-05-08T01:10:23Z
discontinued: false
dominant: false
group u list: _1802_
m user id: 0
owner id: 20667
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: The kanamycin resistance gene tn903 aph from e.coli for use with Synechocystis sp. PCC 6803. The kanamycin gene to allow transformation in Synechocystis sp. PCC 6803 (Poteete et al, 2006).
mutable notes: N/A
mutable provenance: genomic DNA of kanamycin containing strain of Synechocystis sp. PCC 6803
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: kanamucin resistance gene
sequence annotation: Synechocystis RBS + promotor region
sequence: https://synbiohub.org/public/igem/BBa_K1424003_sequence/1

kanamucin resistance gene
type: SequenceAnnotation
persistent identity: annotation2400729
display id: annotation2400729
version: 1
top level: BBa_K1424003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1424003/annotation2400729/range2400729/1

https://synbiohub.org/public/igem/BBa_K1424003/annotation2400729/range2400729/1
type: Range
persistent identity: range2400729
display id: range2400729
version: 1
direction: direction/forward
top level: BBa_K1424003
start: 120
end: 920
orientation: inline

Synechocystis RBS + promotor region
type: SequenceAnnotation
persistent identity: annotation2400731
display id: annotation2400731
version: 1
top level: BBa_K1424003
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1424003/annotation2400731/range2400731/1

https://synbiohub.org/public/igem/BBa_K1424003/annotation2400731/range2400731/1
type: Range
persistent identity: range2400731
display id: range2400731
version: 1
direction: direction/forward
top level: BBa_K1424003
start: 1
end: 110
orientation: inline

BBa_K1321342
type: ComponentDefinition
persistent identity: BBa K1321342
display id: BBa_K1321342
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: sfGFP fused to CBDcex in RFC 25
creator: Chris N Micklem
created: 2014-10-07T11:00:00Z
modified: 2015-06-17T12:26:42Z
discontinued: false
dominant: false
group u list: _1696_
m user id: 4206
owner id: 20830
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: sadf
mutable notes: asdf
mutable provenance: asdf
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1321337
component: BBa_K1321003
component: BBa_B0105
sequence annotation: BBa_B0105
sequence annotation: BBa_K1321337
sequence annotation: BBa_K1321003
sequence: https://synbiohub.org/public/igem/BBa_K1321342_sequence/1

BBa_K1321337
type: Component
persistent identity: component2415359
display id: component2415359
version: 1
top level: BBa_K1321342
access: public
definition: BBa_K1321337

BBa_K1321003
type: Component
persistent identity: component2415362
display id: component2415362
version: 1
top level: BBa_K1321342
access: public
definition: https://synbiohub.org/public/igem/BBa_K1321003/1

BBa_B0105
type: Component
persistent identity: component2415361
display id: component2415361
version: 1
top level: BBa_K1321342
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2415361
display id: annotation2415361
version: 1
top level: BBa_K1321342
location: https://synbiohub.org/public/igem/BBa_K1321342/annotation2415361/range2415361/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_K1321342/annotation2415361/range2415361/1
type: Range
persistent identity: range2415361
display id: range2415361
version: 1
direction: direction/reverse
top level: BBa_K1321342
start: 712
end: 717
orientation: inline

BBa_K1321337
type: SequenceAnnotation
persistent identity: annotation2415359
display id: annotation2415359
version: 1
top level: BBa_K1321342
location: https://synbiohub.org/public/igem/BBa_K1321342/annotation2415359/range2415359/1
component: BBa_K1321337

https://synbiohub.org/public/igem/BBa_K1321342/annotation2415359/range2415359/1
type: Range
persistent identity: range2415359
display id: range2415359
version: 1
direction: direction/reverse
top level: BBa_K1321342
start: 1
end: 711
orientation: inline

BBa_K1321003
type: SequenceAnnotation
persistent identity: annotation2415362
display id: annotation2415362
version: 1
top level: BBa_K1321342
location: https://synbiohub.org/public/igem/BBa_K1321342/annotation2415362/range2415362/1
component: BBa_K1321003

https://synbiohub.org/public/igem/BBa_K1321342/annotation2415362/range2415362/1
type: Range
persistent identity: range2415362
display id: range2415362
version: 1
direction: direction/reverse
top level: BBa_K1321342
start: 718
end: 1044
orientation: inline

BBa_K1349004
type: ComponentDefinition
persistent identity: BBa K1349004
display id: BBa_K1349004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: mesh1
creator: Alexia Satouf, Clara Bouyx, Aimeric Agaoua, Lambert Antoni, Vincent Castel
created: 2014-10-05T11:00:00Z
modified: 2015-05-08T01:10:01Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1724_
m user id: 0
owner id: 6328
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Mesh1 is involved in ppGpp hydrolysis.
mutable notes: xxx
mutable provenance: xxx
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1349004_sequence/1

BBa_K1315010
type: ComponentDefinition
persistent identity: BBa K1315010
display id: BBa_K1315010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pseudomonas AutoInducer 1 (PAI-1) Biosensor
creator: Dimitrios Michailidis
created: 2014-09-25T11:00:00Z
modified: 2015-05-08T01:09:50Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _1690_
m user id: 0
owner id: 21812
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is a composite part that consists of 4 biobricks. This is an alternative PA1 Activated System from Bba_K1315009 that instead uses pluxR (Bba_R0062). In the front it has ptet(Bba_R0040) which is constitutively on. This produces LasR all the time with lasR(Bba_C0179) next in the sequence. Only in the presence of the Pseudomonas Autoinducer 1 (PA1), LasR forms an complex with PA1 and it can then activate the pluxR(Bba_R0062) promoter that leads to the production of GFP(Bba_E0040).
mutable notes: All the inserts were digested with XbaI and PstI, and all the vectors were each time cut with SpeI and PstI.
mutable provenance: All the parts have come from existing iGEM distribution kits.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_E0040
component: BBa_C0179
component: BBa_R0040
component: BBa_R0062
sequence annotation: BBa_R0040
sequence annotation: BBa_C0179
sequence annotation: BBa_E0040
sequence annotation: BBa_R0062
sequence: https://synbiohub.org/public/igem/BBa_K1315010_sequence/1

BBa_E0040
type: Component
persistent identity: component2387075
display id: component2387075
version: 1
top level: BBa_K1315010
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_C0179
type: Component
persistent identity: component2387068
display id: component2387068
version: 1
top level: BBa_K1315010
access: public
definition: https://synbiohub.org/public/igem/BBa_C0179/1

BBa_R0040
type: Component
persistent identity: component2387063
display id: component2387063
version: 1
top level: BBa_K1315010
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_R0062
type: Component
persistent identity: component2387070
display id: component2387070
version: 1
top level: BBa_K1315010
access: public
definition: https://synbiohub.org/public/igem/BBa_R0062/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2387063
display id: annotation2387063
version: 1
top level: BBa_K1315010
location: https://synbiohub.org/public/igem/BBa_K1315010/annotation2387063/range2387063/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K1315010/annotation2387063/range2387063/1
type: Range
persistent identity: range2387063
display id: range2387063
version: 1
direction: direction/forward
top level: BBa_K1315010
start: 1
end: 54
orientation: inline

BBa_C0179
type: SequenceAnnotation
persistent identity: annotation2387068
display id: annotation2387068
version: 1
top level: BBa_K1315010
location: https://synbiohub.org/public/igem/BBa_K1315010/annotation2387068/range2387068/1
component: BBa_C0179

https://synbiohub.org/public/igem/BBa_K1315010/annotation2387068/range2387068/1
type: Range
persistent identity: range2387068
display id: range2387068
version: 1
direction: direction/reverse
top level: BBa_K1315010
start: 61
end: 783
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2387075
display id: annotation2387075
version: 1
top level: BBa_K1315010
location: https://synbiohub.org/public/igem/BBa_K1315010/annotation2387075/range2387075/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K1315010/annotation2387075/range2387075/1
type: Range
persistent identity: range2387075
display id: range2387075
version: 1
direction: direction/reverse
top level: BBa_K1315010
start: 853
end: 1572
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation2387070
display id: annotation2387070
version: 1
top level: BBa_K1315010
location: https://synbiohub.org/public/igem/BBa_K1315010/annotation2387070/range2387070/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_K1315010/annotation2387070/range2387070/1
type: Range
persistent identity: range2387070
display id: range2387070
version: 1
direction: direction/forward
top level: BBa_K1315010
start: 792
end: 846
orientation: inline

BBa_K1640012
type: ComponentDefinition
persistent identity: BBa K1640012
display id: BBa_K1640012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: psbQR
creator: Meghan Cook
created: 2015-09-09T11:00:00Z
modified: 2015-09-10T01:02:41Z
discontinued: false
dominant: false
group u list: _2057_
m user id: 16353
owner id: 28565
owning group id: 9
status: status/Planning
bookmark: false
mutable description: n/a
mutable notes: n/a
mutable provenance: n/a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: double terminator
sequence annotation: psbQ
sequence annotation: rbs
sequence annotation: psbR
sequence: https://synbiohub.org/public/igem/BBa_K1640012_sequence/1

double terminator
type: SequenceAnnotation
persistent identity: annotation2448238
display id: annotation2448238
version: 1
top level: BBa_K1640012
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1640012/annotation2448238/range2448238/1

https://synbiohub.org/public/igem/BBa_K1640012/annotation2448238/range2448238/1
type: Range
persistent identity: range2448238
display id: range2448238
version: 1
direction: direction/forward
top level: BBa_K1640012
start: 913
end: 1041
orientation: inline

psbQ
type: SequenceAnnotation
persistent identity: annotation2448234
display id: annotation2448234
version: 1
top level: BBa_K1640012
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1640012/annotation2448234/range2448234/1

https://synbiohub.org/public/igem/BBa_K1640012/annotation2448234/range2448234/1
type: Range
persistent identity: range2448234
display id: range2448234
version: 1
direction: direction/forward
top level: BBa_K1640012
start: 20
end: 553
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2448236
display id: annotation2448236
version: 1
top level: BBa_K1640012
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1640012/annotation2448236/range2448236/1

https://synbiohub.org/public/igem/BBa_K1640012/annotation2448236/range2448236/1
type: Range
persistent identity: range2448236
display id: range2448236
version: 1
direction: direction/forward
top level: BBa_K1640012
start: 554
end: 570
orientation: inline

psbR
type: SequenceAnnotation
persistent identity: annotation2448237
display id: annotation2448237
version: 1
top level: BBa_K1640012
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1640012/annotation2448237/range2448237/1

https://synbiohub.org/public/igem/BBa_K1640012/annotation2448237/range2448237/1
type: Range
persistent identity: range2448237
display id: range2448237
version: 1
direction: direction/forward
top level: BBa_K1640012
start: 571
end: 912
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2448233
display id: annotation2448233
version: 1
top level: BBa_K1640012
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1640012/annotation2448233/range2448233/1

https://synbiohub.org/public/igem/BBa_K1640012/annotation2448233/range2448233/1
type: Range
persistent identity: range2448233
display id: range2448233
version: 1
direction: direction/forward
top level: BBa_K1640012
start: 1
end: 19
orientation: inline

BBa_K1321138
type: ComponentDefinition
persistent identity: BBa K1321138
display id: BBa_K1321138
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Metallothionein SmtA with T7 promoter
creator: Gabriella Santosa
created: 2014-10-08T11:00:00Z
modified: 2015-05-08T01:09:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 0
owner id: 20961
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: GATC
mutable notes: GATC
mutable provenance: GATC
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1321011
component: BBa_K1319106
component: BBa_K1321338
sequence annotation: BBa_K1319106
sequence annotation: BBa_K1321338
sequence annotation: BBa_K1321011
sequence: https://synbiohub.org/public/igem/BBa_K1321138_sequence/1

BBa_K1321011
type: Component
persistent identity: component2414817
display id: component2414817
version: 1
top level: BBa_K1321138
access: public
definition: https://synbiohub.org/public/igem/BBa_K1321011/1

BBa_K1319106
type: Component
persistent identity: component2414815
display id: component2414815
version: 1
top level: BBa_K1321138
access: public
definition: https://synbiohub.org/public/igem/BBa_K1319106/1

BBa_K1321338
type: Component
persistent identity: component2414813
display id: component2414813
version: 1
top level: BBa_K1321138
access: public
definition: BBa_K1321338

BBa_K1319106
type: SequenceAnnotation
persistent identity: annotation2414815
display id: annotation2414815
version: 1
top level: BBa_K1321138
location: https://synbiohub.org/public/igem/BBa_K1321138/annotation2414815/range2414815/1
component: BBa_K1319106

https://synbiohub.org/public/igem/BBa_K1321138/annotation2414815/range2414815/1
type: Range
persistent identity: range2414815
display id: range2414815
version: 1
direction: direction/reverse
top level: BBa_K1321138
start: 44
end: 58
orientation: inline

BBa_K1321338
type: SequenceAnnotation
persistent identity: annotation2414813
display id: annotation2414813
version: 1
top level: BBa_K1321138
location: https://synbiohub.org/public/igem/BBa_K1321138/annotation2414813/range2414813/1
component: BBa_K1321338

https://synbiohub.org/public/igem/BBa_K1321138/annotation2414813/range2414813/1
type: Range
persistent identity: range2414813
display id: range2414813
version: 1
direction: direction/reverse
top level: BBa_K1321138
start: 1
end: 43
orientation: inline

BBa_K1321011
type: SequenceAnnotation
persistent identity: annotation2414817
display id: annotation2414817
version: 1
top level: BBa_K1321138
location: https://synbiohub.org/public/igem/BBa_K1321138/annotation2414817/range2414817/1
component: BBa_K1321011

https://synbiohub.org/public/igem/BBa_K1321138/annotation2414817/range2414817/1
type: Range
persistent identity: range2414817
display id: range2414817
version: 1
direction: direction/reverse
top level: BBa_K1321138
start: 59
end: 223
orientation: inline

BBa_K1632012
type: ComponentDefinition
persistent identity: BBa K1632012
display id: BBa_K1632012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PBAD/araC_rbs_fimB(wild-type)
creator: Riku Shinohara
created: 2015-08-29T11:00:00Z
modified: 2015-09-18T09:40:16Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2049_
m user id: 24760
owner id: 22687
owning group id: 9
status: status/Planning
bookmark: true
mutable description: a
mutable notes: a
mutable provenance: a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0034
component: BBa_K1632010
component: BBa_I0500
sequence annotation: BBa_I0500
sequence annotation: BBa_B0034
sequence annotation: BBa_K1632010
sequence: https://synbiohub.org/public/igem/BBa_K1632012_sequence/1

BBa_B0034
type: Component
persistent identity: component2440136
display id: component2440136
version: 1
top level: BBa_K1632012
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1632010
type: Component
persistent identity: component2440137
display id: component2440137
version: 1
top level: BBa_K1632012
access: public
definition: BBa_K1632010

BBa_I0500
type: Component
persistent identity: component2440134
display id: component2440134
version: 1
top level: BBa_K1632012
access: public
definition: pBad/araC

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2440134
display id: annotation2440134
version: 1
top level: BBa_K1632012
location: https://synbiohub.org/public/igem/BBa_K1632012/annotation2440134/range2440134/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_K1632012/annotation2440134/range2440134/1
type: Range
persistent identity: range2440134
display id: range2440134
version: 1
direction: direction/reverse
top level: BBa_K1632012
start: 1
end: 1210
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2440136
display id: annotation2440136
version: 1
top level: BBa_K1632012
location: https://synbiohub.org/public/igem/BBa_K1632012/annotation2440136/range2440136/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1632012/annotation2440136/range2440136/1
type: Range
persistent identity: range2440136
display id: range2440136
version: 1
direction: direction/reverse
top level: BBa_K1632012
start: 1219
end: 1230
orientation: inline

BBa_K1632010
type: SequenceAnnotation
persistent identity: annotation2440137
display id: annotation2440137
version: 1
top level: BBa_K1632012
location: https://synbiohub.org/public/igem/BBa_K1632012/annotation2440137/range2440137/1
component: BBa_K1632010

https://synbiohub.org/public/igem/BBa_K1632012/annotation2440137/range2440137/1
type: Range
persistent identity: range2440137
display id: range2440137
version: 1
direction: direction/reverse
top level: BBa_K1632012
start: 1237
end: 1839
orientation: inline

BBa_K1478001
type: ComponentDefinition
persistent identity: BBa K1478001
display id: BBa_K1478001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cellulose binding domain
creator: Fabian Fr??mling
created: 2014-09-12T11:00:00Z
modified: 2015-06-17T01:26:18Z
discontinued: false
dominant: false
group u list: _1858_
m user id: 4206
owner id: 20973
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Coding region for cellulose binding domain. When fused to a protein, protein attaches to cellulose
mutable notes: Sequence was optimized for Escherichia coli and Arabidopsis thaliana codon usage.
mutable provenance: Domain derived from cellulose binding protein from Chlostridium cellulovorans(WP_013291799).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K1478001_sequence/1

BBa_K1399011
type: ComponentDefinition
persistent identity: BBa K1399011
display id: BBa_K1399011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Plac-RFP(LVA)
creator: Anna Stikane
created: 2014-09-18T11:00:00Z
modified: 2015-05-08T01:10:16Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1777_
m user id: 0
owner id: 22477
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: Lactose/IPTG inducible promoter with RBS, RFP reporter tagged with LVA-ssrA degradation tag and terminator. The tagged RFP is actively degraded within cell, thus provides better temporal resolution of red fluorescence and promoter activity. This part can be considered as improved version of BBa_J04451 as it does not contain StuI restriction site coding for additional 'RP' residues between RFP and LVA tag.
mutable notes: Part was assembled using BrickClip assembly (BBF RFC 104) using other biobrick parts as templates. BrickClip assembly is a special case of more general Paperclip assembly method.[1]
mutable provenance: other biobricks
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: BBa_R0010
sequence annotation: -10
sequence annotation: polya
sequence annotation: BBa_B0010
sequence annotation: -35
sequence annotation: BBa_B0015
sequence annotation: BBa_K1399001
sequence annotation: LVA-ssrA tag
sequence annotation: stop
sequence annotation: end of LacI coding region (inactive)
sequence annotation: stem_loop
sequence annotation: T7 TE
sequence annotation: BBa_B0034
sequence annotation: BBa_B0012
sequence annotation: CAP binding site
sequence annotation: conserved
sequence annotation: start
sequence annotation: Barcode of BBa_E1010
sequence annotation: LacI binding site
sequence annotation: BBa_E1010 w/o stop
sequence: https://synbiohub.org/public/igem/BBa_K1399011_sequence/1

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2386289
display id: annotation2386289
version: 1
top level: BBa_K1399011
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386289/range2386289/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386289/range2386289/1
type: Range
persistent identity: range2386289
display id: range2386289
version: 1
direction: direction/forward
top level: BBa_K1399011
start: 1
end: 200
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2386293
display id: annotation2386293
version: 1
top level: BBa_K1399011
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386293/range2386293/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386293/range2386293/1
type: Range
persistent identity: range2386293
display id: range2386293
version: 1
direction: direction/forward
top level: BBa_K1399011
start: 161
end: 166
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation2386310
display id: annotation2386310
version: 1
top level: BBa_K1399011
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386310/range2386310/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386310/range2386310/1
type: Range
persistent identity: range2386310
display id: range2386310
version: 1
direction: direction/forward
top level: BBa_K1399011
start: 1089
end: 1102
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2386306
display id: annotation2386306
version: 1
top level: BBa_K1399011
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386306/range2386306/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386306/range2386306/1
type: Range
persistent identity: range2386306
display id: range2386306
version: 1
direction: direction/forward
top level: BBa_K1399011
start: 974
end: 1053
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2386292
display id: annotation2386292
version: 1
top level: BBa_K1399011
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386292/range2386292/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386292/range2386292/1
type: Range
persistent identity: range2386292
display id: range2386292
version: 1
direction: direction/forward
top level: BBa_K1399011
start: 137
end: 142
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2386305
display id: annotation2386305
version: 1
top level: BBa_K1399011
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386305/range2386305/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386305/range2386305/1
type: Range
persistent identity: range2386305
display id: range2386305
version: 1
direction: direction/reverse
top level: BBa_K1399011
start: 974
end: 1102
orientation: inline

BBa_K1399001
type: SequenceAnnotation
persistent identity: annotation2386299
display id: annotation2386299
version: 1
top level: BBa_K1399011
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386299/range2386299/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386299/range2386299/1
type: Range
persistent identity: range2386299
display id: range2386299
version: 1
direction: direction/reverse
top level: BBa_K1399011
start: 227
end: 940
orientation: inline

LVA-ssrA tag
type: SequenceAnnotation
persistent identity: annotation2386301
display id: annotation2386301
version: 1
top level: BBa_K1399011
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386301/range2386301/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386301/range2386301/1
type: Range
persistent identity: range2386301
display id: range2386301
version: 1
direction: direction/forward
top level: BBa_K1399011
start: 902
end: 934
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2386303
display id: annotation2386303
version: 1
top level: BBa_K1399011
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386303/range2386303/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386303/range2386303/1
type: Range
persistent identity: range2386303
display id: range2386303
version: 1
direction: direction/forward
top level: BBa_K1399011
start: 938
end: 940
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2386311
display id: annotation2386311
version: 1
top level: BBa_K1399011
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386311/range2386311/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386311/range2386311/1
type: Range
persistent identity: range2386311
display id: range2386311
version: 1
direction: direction/forward
top level: BBa_K1399011
start: 1095
end: 1095
orientation: inline

end of LacI coding region (inactive)
type: SequenceAnnotation
persistent identity: annotation2386290
display id: annotation2386290
version: 1
top level: BBa_K1399011
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386290/range2386290/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386290/range2386290/1
type: Range
persistent identity: range2386290
display id: range2386290
version: 1
direction: direction/forward
top level: BBa_K1399011
start: 1
end: 88
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2386302
display id: annotation2386302
version: 1
top level: BBa_K1399011
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386302/range2386302/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386302/range2386302/1
type: Range
persistent identity: range2386302
display id: range2386302
version: 1
direction: direction/forward
top level: BBa_K1399011
start: 935
end: 937
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation2386307
display id: annotation2386307
version: 1
top level: BBa_K1399011
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386307/range2386307/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386307/range2386307/1
type: Range
persistent identity: range2386307
display id: range2386307
version: 1
direction: direction/forward
top level: BBa_K1399011
start: 985
end: 1028
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation2386309
display id: annotation2386309
version: 1
top level: BBa_K1399011
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386309/range2386309/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386309/range2386309/1
type: Range
persistent identity: range2386309
display id: range2386309
version: 1
direction: direction/forward
top level: BBa_K1399011
start: 1069
end: 1088
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2386296
display id: annotation2386296
version: 1
top level: BBa_K1399011
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386296/range2386296/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386296/range2386296/1
type: Range
persistent identity: range2386296
display id: range2386296
version: 1
direction: direction/reverse
top level: BBa_K1399011
start: 209
end: 220
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2386308
display id: annotation2386308
version: 1
top level: BBa_K1399011
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386308/range2386308/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386308/range2386308/1
type: Range
persistent identity: range2386308
display id: range2386308
version: 1
direction: direction/forward
top level: BBa_K1399011
start: 1062
end: 1102
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation2386291
display id: annotation2386291
version: 1
top level: BBa_K1399011
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386291/range2386291/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386291/range2386291/1
type: Range
persistent identity: range2386291
display id: range2386291
version: 1
direction: direction/forward
top level: BBa_K1399011
start: 89
end: 126
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation2386297
display id: annotation2386297
version: 1
top level: BBa_K1399011
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386297/range2386297/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386297/range2386297/1
type: Range
persistent identity: range2386297
display id: range2386297
version: 1
direction: direction/forward
top level: BBa_K1399011
start: 213
end: 216
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2386298
display id: annotation2386298
version: 1
top level: BBa_K1399011
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386298/range2386298/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386298/range2386298/1
type: Range
persistent identity: range2386298
display id: range2386298
version: 1
direction: direction/forward
top level: BBa_K1399011
start: 227
end: 229
orientation: inline

Barcode of BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2386304
display id: annotation2386304
version: 1
top level: BBa_K1399011
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386304/range2386304/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386304/range2386304/1
type: Range
persistent identity: range2386304
display id: range2386304
version: 1
direction: direction/forward
top level: BBa_K1399011
start: 941
end: 965
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation2386294
display id: annotation2386294
version: 1
top level: BBa_K1399011
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386294/range2386294/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386294/range2386294/1
type: Range
persistent identity: range2386294
display id: range2386294
version: 1
direction: direction/forward
top level: BBa_K1399011
start: 166
end: 200
orientation: inline

BBa_E1010 w/o stop
type: SequenceAnnotation
persistent identity: annotation2386300
display id: annotation2386300
version: 1
top level: BBa_K1399011
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386300/range2386300/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386300/range2386300/1
type: Range
persistent identity: range2386300
display id: range2386300
version: 1
direction: direction/forward
top level: BBa_K1399011
start: 230
end: 901
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2386295
display id: annotation2386295
version: 1
top level: BBa_K1399011
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386295/range2386295/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386295/range2386295/1
type: Range
persistent identity: range2386295
display id: range2386295
version: 1
direction: direction/forward
top level: BBa_K1399011
start: 173
end: 173
orientation: inline

TerDnaE3(N
type: ComponentDefinition
persistent identity: BBa K1362130
display id: BBa_K1362130
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS + TerDnaE3 N-intein RFC[105] assembly construct (with His6)
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B??scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch??fer, Carolin Schmelas, Silvan Schmitz, Max Waldha
created: 2014-10-08T11:00:00Z
modified: 2015-05-08T01:10:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1738_
m user id: 0
owner id: 12377
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: ???
mutable notes: ???
mutable provenance: ???
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_G0000
component: BBa_K1362422
component: BBa_K1362090
component: BBa_K1362415
component: BBa_K1362414
component: BBa_K1362404
component: BBa_K1362417
component: BBa_K1362421
component: BBa_K1362437
component: BBa_K1362430
sequence annotation: BBa_K1362090
sequence annotation: BBa_K1362404
sequence annotation: BBa_G0000
sequence annotation: BBa_K1362417
sequence annotation: BBa_K1362414
sequence annotation: BBa_K1362437
sequence annotation: BBa_K1362422
sequence annotation: BBa_K1362415
sequence annotation: BBa_K1362430
sequence annotation: BBa_K1362421
sequence: https://synbiohub.org/public/igem/BBa_K1362130_sequence/1

BBa_G0000
type: Component
persistent identity: component2410472
display id: component2410472
version: 1
top level: TerDnaE3(N
access: public
definition: https://synbiohub.org/public/igem/BBa_G0000/1

BBa_K1362422
type: Component
persistent identity: component2410494
display id: component2410494
version: 1
top level: TerDnaE3(N
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362422/1

BBa_K1362090
type: Component
persistent identity: component2410471
display id: component2410471
version: 1
top level: TerDnaE3(N
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362090/1

BBa_K1362415
type: Component
persistent identity: component2410495
display id: component2410495
version: 1
top level: TerDnaE3(N
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362415/1

BBa_K1362414
type: Component
persistent identity: component2410473
display id: component2410473
version: 1
top level: TerDnaE3(N
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362414/1

BBa_K1362404
type: Component
persistent identity: component2410496
display id: component2410496
version: 1
top level: TerDnaE3(N
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362404/1

BBa_K1362417
type: Component
persistent identity: component2410499
display id: component2410499
version: 1
top level: TerDnaE3(N
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362417/1

BBa_K1362421
type: Component
persistent identity: component2410504
display id: component2410504
version: 1
top level: TerDnaE3(N
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362421/1

BBa_K1362437
type: Component
persistent identity: component2410506
display id: component2410506
version: 1
top level: TerDnaE3(N
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362437/1

BBa_K1362430
type: Component
persistent identity: component2410502
display id: component2410502
version: 1
top level: TerDnaE3(N
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362430/1

BBa_K1362090
type: SequenceAnnotation
persistent identity: annotation2410471
display id: annotation2410471
version: 1
top level: TerDnaE3(N
location: https://synbiohub.org/public/igem/BBa_K1362130/annotation2410471/range2410471/1
component: BBa_K1362090

https://synbiohub.org/public/igem/BBa_K1362130/annotation2410471/range2410471/1
type: Range
persistent identity: range2410471
display id: range2410471
version: 1
direction: direction/reverse
top level: TerDnaE3(N
start: 1
end: 29
orientation: inline

BBa_K1362404
type: SequenceAnnotation
persistent identity: annotation2410496
display id: annotation2410496
version: 1
top level: TerDnaE3(N
location: https://synbiohub.org/public/igem/BBa_K1362130/annotation2410496/range2410496/1
component: BBa_K1362404

https://synbiohub.org/public/igem/BBa_K1362130/annotation2410496/range2410496/1
type: Range
persistent identity: range2410496
display id: range2410496
version: 1
direction: direction/reverse
top level: TerDnaE3(N
start: 1126
end: 1511
orientation: inline

BBa_G0000
type: SequenceAnnotation
persistent identity: annotation2410472
display id: annotation2410472
version: 1
top level: TerDnaE3(N
location: https://synbiohub.org/public/igem/BBa_K1362130/annotation2410472/range2410472/1
component: BBa_G0000

https://synbiohub.org/public/igem/BBa_K1362130/annotation2410472/range2410472/1
type: Range
persistent identity: range2410472
display id: range2410472
version: 1
direction: direction/reverse
top level: TerDnaE3(N
start: 30
end: 35
orientation: inline

BBa_K1362417
type: SequenceAnnotation
persistent identity: annotation2410499
display id: annotation2410499
version: 1
top level: TerDnaE3(N
location: https://synbiohub.org/public/igem/BBa_K1362130/annotation2410499/range2410499/1
component: BBa_K1362417

https://synbiohub.org/public/igem/BBa_K1362130/annotation2410499/range2410499/1
type: Range
persistent identity: range2410499
display id: range2410499
version: 1
direction: direction/reverse
top level: TerDnaE3(N
start: 1512
end: 1517
orientation: inline

BBa_K1362414
type: SequenceAnnotation
persistent identity: annotation2410473
display id: annotation2410473
version: 1
top level: TerDnaE3(N
location: https://synbiohub.org/public/igem/BBa_K1362130/annotation2410473/range2410473/1
component: BBa_K1362414

https://synbiohub.org/public/igem/BBa_K1362130/annotation2410473/range2410473/1
type: Range
persistent identity: range2410473
display id: range2410473
version: 1
direction: direction/reverse
top level: TerDnaE3(N
start: 36
end: 38
orientation: inline

BBa_K1362437
type: SequenceAnnotation
persistent identity: annotation2410506
display id: annotation2410506
version: 1
top level: TerDnaE3(N
location: https://synbiohub.org/public/igem/BBa_K1362130/annotation2410506/range2410506/1
component: BBa_K1362437

https://synbiohub.org/public/igem/BBa_K1362130/annotation2410506/range2410506/1
type: Range
persistent identity: range2410506
display id: range2410506
version: 1
direction: direction/reverse
top level: TerDnaE3(N
start: 1546
end: 1548
orientation: inline

BBa_K1362422
type: SequenceAnnotation
persistent identity: annotation2410494
display id: annotation2410494
version: 1
top level: TerDnaE3(N
location: https://synbiohub.org/public/igem/BBa_K1362130/annotation2410494/range2410494/1
component: BBa_K1362422

https://synbiohub.org/public/igem/BBa_K1362130/annotation2410494/range2410494/1
type: Range
persistent identity: range2410494
display id: range2410494
version: 1
direction: direction/reverse
top level: TerDnaE3(N
start: 39
end: 1121
orientation: inline

BBa_K1362415
type: SequenceAnnotation
persistent identity: annotation2410495
display id: annotation2410495
version: 1
top level: TerDnaE3(N
location: https://synbiohub.org/public/igem/BBa_K1362130/annotation2410495/range2410495/1
component: BBa_K1362415

https://synbiohub.org/public/igem/BBa_K1362130/annotation2410495/range2410495/1
type: Range
persistent identity: range2410495
display id: range2410495
version: 1
direction: direction/reverse
top level: TerDnaE3(N
start: 1122
end: 1125
orientation: inline

BBa_K1362430
type: SequenceAnnotation
persistent identity: annotation2410502
display id: annotation2410502
version: 1
top level: TerDnaE3(N
location: https://synbiohub.org/public/igem/BBa_K1362130/annotation2410502/range2410502/1
component: BBa_K1362430

https://synbiohub.org/public/igem/BBa_K1362130/annotation2410502/range2410502/1
type: Range
persistent identity: range2410502
display id: range2410502
version: 1
direction: direction/reverse
top level: TerDnaE3(N
start: 1518
end: 1541
orientation: inline

BBa_K1362421
type: SequenceAnnotation
persistent identity: annotation2410504
display id: annotation2410504
version: 1
top level: TerDnaE3(N
location: https://synbiohub.org/public/igem/BBa_K1362130/annotation2410504/range2410504/1
component: BBa_K1362421

https://synbiohub.org/public/igem/BBa_K1362130/annotation2410504/range2410504/1
type: Range
persistent identity: range2410504
display id: range2410504
version: 1
direction: direction/reverse
top level: TerDnaE3(N
start: 1542
end: 1545
orientation: inline

BBa_K1445001
type: ComponentDefinition
persistent identity: BBa K1445001
display id: BBa_K1445001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Endogenous Type II CRISPR-Cas9 phagemid
creator: Josephina Hendrix
created: 2014-09-24T11:00:00Z
modified: 2015-05-08T01:10:27Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1823_
m user id: 0
owner id: 22016
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: The M13ori-pCas9 composite part consists of the M13 origin of replication (M13ori) and the pCas9 part (BBa_K1218011) containing a tracrRNA sequence, the cas9 gene, and a minimal CRISPR array. M13ori (BBa_K1445000) is a 500bp noncoding sequence that is recognized by the gene II of the M13 phage, which results in the uptake of the plasmid containing the ori into an assembled phage coat. This part does not contain any complete phage genes so phage production requires a Helper Phagemid such as M13K07. There will be some packaging of Helper Phagemid but their packaging signal weakened so parts containing the M13ori are preferentially packaged. pCas9 contains the native trcrRNA and promoter region upstream of the Cas9 protein. The type II Cas9 protein is the native, active version from streptococcus pyogenes with the exceptrion of a SNP to erase an illegal EcoRI cleavage site. Downstream of the Cas9 protein is a minimal CRISPR array. This minimal array only includes two CRISPR repeats flanking a spacer region. It is this spacer region that determines which DNA sequence is targeted by the Cas9 endonuclease. Spacer sequence can be easily replaced with the protocol found here.
mutable notes: We have experienced some toxic effects by the pCas9 part so we suggest growing part-containing cells in lower concentrations of antibiotic, even if plasmid contains a high copy origin of replication.
mutable provenance: The M13 origin of replication from Litmus28i and part BBa_K1218011 from the registry.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1445000
component: BBa_K1218011
sequence annotation: BBa_K1218011
sequence annotation: BBa_K1445000
sequence: https://synbiohub.org/public/igem/BBa_K1445001_sequence/1

BBa_K1445000
type: Component
persistent identity: component2386528
display id: component2386528
version: 1
top level: BBa_K1445001
access: public
definition: BBa_K1445000

BBa_K1218011
type: Component
persistent identity: component2386529
display id: component2386529
version: 1
top level: BBa_K1445001
access: public
definition: https://synbiohub.org/public/igem/BBa_K1218011/1

BBa_K1218011
type: SequenceAnnotation
persistent identity: annotation2386529
display id: annotation2386529
version: 1
top level: BBa_K1445001
location: https://synbiohub.org/public/igem/BBa_K1445001/annotation2386529/range2386529/1
component: BBa_K1218011

https://synbiohub.org/public/igem/BBa_K1445001/annotation2386529/range2386529/1
type: Range
persistent identity: range2386529
display id: range2386529
version: 1
direction: direction/reverse
top level: BBa_K1445001
start: 538
end: 5617
orientation: inline

BBa_K1445000
type: SequenceAnnotation
persistent identity: annotation2386528
display id: annotation2386528
version: 1
top level: BBa_K1445001
location: https://synbiohub.org/public/igem/BBa_K1445001/annotation2386528/range2386528/1
component: BBa_K1445000

https://synbiohub.org/public/igem/BBa_K1445001/annotation2386528/range2386528/1
type: Range
persistent identity: range2386528
display id: range2386528
version: 1
direction: direction/reverse
top level: BBa_K1445001
start: 1
end: 529
orientation: inline

BBa_K1724000
type: ComponentDefinition
persistent identity: BBa K1724000
display id: BBa_K1724000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pcada
creator: Zhanru Chen
created: 2015-09-04T11:00:00Z
modified: 2016-01-11T03:19:05Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2144_
m user id: 4206
owner id: 21980
owning group id: 9
status: status/Planning
bookmark: true
mutable description: cada promoter
mutable notes: FP:GTTTCTTCGAATTCGCGGCCGCTTCTAGATTGACTCTGTAGTTGCTACA VP:GTTTCTTCCTGCAGCGGCCGCTACTAGT ATTGCACACC CTGTAGCAAC TACAGA
mutable provenance: OVERLAP PCR
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: cadmium activated promoter
sequence: https://synbiohub.org/public/igem/BBa_K1724000_sequence/1

cadmium activated promoter
type: SequenceAnnotation
persistent identity: annotation2443820
display id: annotation2443820
version: 1
top level: BBa_K1724000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1724000/annotation2443820/range2443820/1

https://synbiohub.org/public/igem/BBa_K1724000/annotation2443820/range2443820/1
type: Range
persistent identity: range2443820
display id: range2443820
version: 1
direction: direction/forward
top level: BBa_K1724000
start: 1
end: 31
orientation: inline

BBa_K1331002
type: ComponentDefinition
persistent identity: BBa K1331002
display id: BBa_K1331002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RhlR regulatory protein from P. aeruginosa SQ6
creator: Jiayi Dong
created: 2014-09-24T11:00:00Z
modified: 2015-05-08T01:09:56Z
discontinued: false
dominant: false
group u list: _1706_
m user id: 0
owner id: 20167
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Coding a transcription regulator RhlR. RhlR is necessary for transcriptional activation of the rhlAB genes encoding the rhamnosyltransferase. It also functions as a transcriptional activator of elastase structural gene (lasB). RhlR binds to autoinducer molecules BHL (N-butanoyl-L-homoserine lactone), and HHL (N-hexanoyl-L-homoserine lactone). This part includes the noncoding sequence of RhlR.
mutable notes: It includes both the complete coding sequence and part of noncoding sequence of RhlR.
mutable provenance: It comes from the genomic sequence of Pseudomonas aeruginosa SQ6.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: noncoding sequence
sequence annotation: RhlR
sequence: https://synbiohub.org/public/igem/BBa_K1331002_sequence/1

noncoding sequence
type: SequenceAnnotation
persistent identity: annotation2427980
display id: annotation2427980
version: 1
top level: BBa_K1331002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1331002/annotation2427980/range2427980/1

https://synbiohub.org/public/igem/BBa_K1331002/annotation2427980/range2427980/1
type: Range
persistent identity: range2427980
display id: range2427980
version: 1
direction: direction/forward
top level: BBa_K1331002
start: 1
end: 103
orientation: inline

noncoding sequence
type: SequenceAnnotation
persistent identity: annotation2427979
display id: annotation2427979
version: 1
top level: BBa_K1331002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1331002/annotation2427979/range2427979/1

https://synbiohub.org/public/igem/BBa_K1331002/annotation2427979/range2427979/1
type: Range
persistent identity: range2427979
display id: range2427979
version: 1
direction: direction/forward
top level: BBa_K1331002
start: 827
end: 897
orientation: inline

RhlR
type: SequenceAnnotation
persistent identity: annotation2386611
display id: annotation2386611
version: 1
top level: BBa_K1331002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1331002/annotation2386611/range2386611/1

https://synbiohub.org/public/igem/BBa_K1331002/annotation2386611/range2386611/1
type: Range
persistent identity: range2386611
display id: range2386611
version: 1
direction: direction/forward
top level: BBa_K1331002
start: 104
end: 826
orientation: inline

BBa_K1674004
type: ComponentDefinition
persistent identity: BBa K1674004
display id: BBa_K1674004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: zwf - Glucose-6-Phosphate-Dehydrogenase from E.coli
creator: Roni Cohen
created: 2015-09-14T11:00:00Z
modified: 2015-09-18T12:17:43Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2092_
m user id: 25765
owner id: 25765
owning group id: 9
status: status/Planning
bookmark: true
mutable description: ZWF
mutable notes: ZWF
mutable provenance: ZWF
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1674004_sequence/1

BBa_K1758331
type: ComponentDefinition
persistent identity: BBa K1758331
display id: BBa_K1758331
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lead responsive promoter with mRFP
creator: Team Bielefeld-CeBiTec 2015
created: 2015-08-29T11:00:00Z
modified: 2016-01-06T03:42:01Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2180_
m user id: 4206
owner id: 26630
owning group id: 9
status: status/Planning
bookmark: false
mutable description: lead responsive promoter with mRFP for detection
mutable notes: ...
mutable provenance: lead responsive promoter of ????Cupriavidus metallidurans???? CH34
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: mRFP
sequence annotation: pbrA
sequence: https://synbiohub.org/public/igem/BBa_K1758331_sequence/1

mRFP
type: SequenceAnnotation
persistent identity: annotation2440073
display id: annotation2440073
version: 1
top level: BBa_K1758331
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1758331/annotation2440073/range2440073/1

https://synbiohub.org/public/igem/BBa_K1758331/annotation2440073/range2440073/1
type: Range
persistent identity: range2440073
display id: range2440073
version: 1
direction: direction/forward
top level: BBa_K1758331
start: 75
end: 755
orientation: inline

pbrA
type: SequenceAnnotation
persistent identity: annotation2440072
display id: annotation2440072
version: 1
top level: BBa_K1758331
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1758331/annotation2440072/range2440072/1

https://synbiohub.org/public/igem/BBa_K1758331/annotation2440072/range2440072/1
type: Range
persistent identity: range2440072
display id: range2440072
version: 1
direction: direction/forward
top level: BBa_K1758331
start: 1
end: 68
orientation: inline

BBa_K1758106
type: ComponentDefinition
persistent identity: BBa K1758106
display id: BBa_K1758106
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Translation enhancing 5-UTR + mRFP under control of T7 promoter
creator: Team Bielefeld-CeBiTec 2015
created: 2015-08-26T11:00:00Z
modified: 2015-09-18T06:40:02Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2180_
m user id: 26563
owner id: 26563
owning group id: 9
status: status/Planning
bookmark: false
mutable description: mRFP generator ([http://parts.igem.org/Part:BBa_K516030 BBa_K516030]) under control of the T7 promoter ([http://parts.igem.org/Part:BBa_I719005 BBa_I719005]). The part includes a translation enhancing sequence, 5'-UTR, which improves the binding of the mRNA to the ribosome ([http://www.ncbi.nlm.nih.gov/pubmed/2676996 Olins et al. 1989]). The 5'-UTR sequence contains a spacer of 10 adenine bases which has been shown to further enhance translation efficiency ([http://www.ncbi.nlm.nih.gov/pubmed/23927491 Takahashi et al. 2013]).
mutable notes: See [http://parts.igem.org/Part:BBa_K1758102:Design BBa_K1758102]
mutable provenance: This part was obtained via Gibson primers that bind on [http://parts.igem.org/Part:BBa_K1758105 BBa_K1758105].
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_I719005
component: BBa_K1758100
component: BBa_K283035
sequence annotation: BBa_K1758100
sequence annotation: BBa_K283035
sequence annotation: BBa_I719005
sequence: https://synbiohub.org/public/igem/BBa_K1758106_sequence/1

BBa_I719005
type: Component
persistent identity: component2439103
display id: component2439103
version: 1
top level: BBa_K1758106
access: public
definition: https://synbiohub.org/public/igem/BBa_I719005/1

BBa_K1758100
type: Component
persistent identity: component2439106
display id: component2439106
version: 1
top level: BBa_K1758106
access: public
definition: https://synbiohub.org/public/igem/BBa_K1758100/1

BBa_K283035
type: Component
persistent identity: component2439116
display id: component2439116
version: 1
top level: BBa_K1758106
access: public
definition: https://synbiohub.org/public/igem/BBa_K283035/1

BBa_K1758100
type: SequenceAnnotation
persistent identity: annotation2439106
display id: annotation2439106
version: 1
top level: BBa_K1758106
location: https://synbiohub.org/public/igem/BBa_K1758106/annotation2439106/range2439106/1
component: BBa_K1758100

https://synbiohub.org/public/igem/BBa_K1758106/annotation2439106/range2439106/1
type: Range
persistent identity: range2439106
display id: range2439106
version: 1
direction: direction/reverse
top level: BBa_K1758106
start: 24
end: 69
orientation: inline

BBa_K283035
type: SequenceAnnotation
persistent identity: annotation2439116
display id: annotation2439116
version: 1
top level: BBa_K1758106
location: https://synbiohub.org/public/igem/BBa_K1758106/annotation2439116/range2439116/1
component: BBa_K283035

https://synbiohub.org/public/igem/BBa_K1758106/annotation2439116/range2439116/1
type: Range
persistent identity: range2439116
display id: range2439116
version: 1
direction: direction/reverse
top level: BBa_K1758106
start: 70
end: 912
orientation: inline

BBa_I719005
type: SequenceAnnotation
persistent identity: annotation2439103
display id: annotation2439103
version: 1
top level: BBa_K1758106
location: https://synbiohub.org/public/igem/BBa_K1758106/annotation2439103/range2439103/1
component: BBa_I719005

https://synbiohub.org/public/igem/BBa_K1758106/annotation2439103/range2439103/1
type: Range
persistent identity: range2439103
display id: range2439103
version: 1
direction: direction/reverse
top level: BBa_K1758106
start: 1
end: 23
orientation: inline

BBa_K1321366
type: ComponentDefinition
persistent identity: BBa K1321366
display id: BBa_K1321366
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CBDcex fused to sfGFP with T7 promoter
creator: Gabriella Santosa
created: 2014-10-08T11:00:00Z
modified: 2016-01-26T09:46:05Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 4206
owner id: 20961
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: GATC
mutable notes: GATC
mutable provenance: GATC
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1319106
component: BBa_K1321338
component: BBa_K1321349
sequence annotation: BBa_K1321349
sequence annotation: BBa_K1319106
sequence annotation: BBa_K1321338
sequence: https://synbiohub.org/public/igem/BBa_K1321366_sequence/1

BBa_K1319106
type: Component
persistent identity: component2415726
display id: component2415726
version: 1
top level: BBa_K1321366
access: public
definition: https://synbiohub.org/public/igem/BBa_K1319106/1

BBa_K1321338
type: Component
persistent identity: component2415724
display id: component2415724
version: 1
top level: BBa_K1321366
access: public
definition: BBa_K1321338

BBa_K1321349
type: Component
persistent identity: component2415732
display id: component2415732
version: 1
top level: BBa_K1321366
access: public
definition: BBa_K1321349

BBa_K1321349
type: SequenceAnnotation
persistent identity: annotation2415732
display id: annotation2415732
version: 1
top level: BBa_K1321366
location: https://synbiohub.org/public/igem/BBa_K1321366/annotation2415732/range2415732/1
component: BBa_K1321349

https://synbiohub.org/public/igem/BBa_K1321366/annotation2415732/range2415732/1
type: Range
persistent identity: range2415732
display id: range2415732
version: 1
direction: direction/reverse
top level: BBa_K1321366
start: 59
end: 1102
orientation: inline

BBa_K1319106
type: SequenceAnnotation
persistent identity: annotation2415726
display id: annotation2415726
version: 1
top level: BBa_K1321366
location: https://synbiohub.org/public/igem/BBa_K1321366/annotation2415726/range2415726/1
component: BBa_K1319106

https://synbiohub.org/public/igem/BBa_K1321366/annotation2415726/range2415726/1
type: Range
persistent identity: range2415726
display id: range2415726
version: 1
direction: direction/reverse
top level: BBa_K1321366
start: 44
end: 58
orientation: inline

BBa_K1321338
type: SequenceAnnotation
persistent identity: annotation2415724
display id: annotation2415724
version: 1
top level: BBa_K1321366
location: https://synbiohub.org/public/igem/BBa_K1321366/annotation2415724/range2415724/1
component: BBa_K1321338

https://synbiohub.org/public/igem/BBa_K1321366/annotation2415724/range2415724/1
type: Range
persistent identity: range2415724
display id: range2415724
version: 1
direction: direction/reverse
top level: BBa_K1321366
start: 1
end: 43
orientation: inline

BBa_K1445000
type: ComponentDefinition
persistent identity: BBa K1445000
display id: BBa_K1445000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: M13 Ori: Packaging signal for M13 phage
creator: Josephina Hendrix
created: 2014-07-27T11:00:00Z
modified: 2015-05-08T01:10:27Z
discontinued: false
dominant: false
group u list: _1823_
m user id: 0
owner id: 22016
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Inclusion of this part on a plasmid will facilitate the uptake of the plasmid as a single-stranded phagemid into the M13 phage. Note that this part does not code for phage coat proteins so phage containing this M13 ori and your part will not multiply. Therefore, the M13K07 or similar helper phagemid is required for phage production. This part is packaged preferentially over M13K07, reducing the risk of uncontrolled phage multiplication.
mutable notes: Orientation in relation to ori may affect packaging ability. Also, 3??? end contains the promoter for the M13 gene II. This is a strong promoter and may cause unwanted expression of down stream genes. We recommend placing coding regions upstream of part.
mutable provenance: M13 ori from the Litmus 28i vector
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K1445000_sequence/1

SP-aprE
type: ComponentDefinition
persistent identity: BBa K1674000
display id: BBa_K1674000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Secretion signal peptide originated from the B.subtilis extracellular protease AprE
creator: Liron Abrahami
created: 2015-09-03T11:00:00Z
modified: 2015-09-17T08:12:50Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2092_
m user id: 26115
owner id: 26115
owning group id: 9
sample status: In stock
status: status/Available
status: status/Planning
bookmark: true
mutable description: 1
mutable notes: 1
mutable provenance: 1
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K1674000_sequence/1

BBa_K1362204
type: ComponentDefinition
persistent identity: BBa K1362204
display id: BBa_K1362204
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Sortase A non-circularization construct (with His6; without RBS)
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Büscher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schäfer, Carolin Schmelas, Silvan Schmitz, Max Waldha
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:05Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1738_
m user id: 0
owner id: 22951
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: TODO
mutable notes: TODo
mutable provenance: TODO
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1362449
component: BBa_J70594
component: BBa_K1362447
component: BBa_K1362459
component: BBa_K1362453
component: BBa_K1362424
component: BBa_K1362458
component: BBa_J04450
component: BBa_K1362445
sequence annotation: BBa_K1362459
sequence annotation: BBa_K1362447
sequence annotation: BBa_J70594
sequence annotation: BBa_K1362445
sequence annotation: BBa_J04450
sequence annotation: BBa_K1362458
sequence annotation: BBa_K1362453
sequence annotation: BBa_K1362424
sequence annotation: BBa_K1362449
sequence: https://synbiohub.org/public/igem/BBa_K1362204_sequence/1

BBa_K1362449
type: Component
persistent identity: component2410822
display id: component2410822
version: 1
top level: BBa_K1362204
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362449/1

BBa_J70594
type: Component
persistent identity: component2410846
display id: component2410846
version: 1
top level: BBa_K1362204
access: public
definition: https://synbiohub.org/public/igem/BBa_J70594/1

BBa_K1362447
type: Component
persistent identity: component2410824
display id: component2410824
version: 1
top level: BBa_K1362204
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362447/1

BBa_K1362459
type: Component
persistent identity: component2410844
display id: component2410844
version: 1
top level: BBa_K1362204
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362459/1

BBa_K1362453
type: Component
persistent identity: component2410823
display id: component2410823
version: 1
top level: BBa_K1362204
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362453/1

BBa_K1362424
type: Component
persistent identity: component2410843
display id: component2410843
version: 1
top level: BBa_K1362204
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362424/1

BBa_K1362458
type: Component
persistent identity: component2410821
display id: component2410821
version: 1
top level: BBa_K1362204
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362458/1

BBa_J04450
type: Component
persistent identity: component2410842
display id: component2410842
version: 1
top level: BBa_K1362204
access: public
definition: BBa_J04450

BBa_K1362445
type: Component
persistent identity: component2410820
display id: component2410820
version: 1
top level: BBa_K1362204
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362445/1

BBa_K1362459
type: SequenceAnnotation
persistent identity: annotation2410844
display id: annotation2410844
version: 1
top level: BBa_K1362204
location: https://synbiohub.org/public/igem/BBa_K1362204/annotation2410844/range2410844/1
component: BBa_K1362459

https://synbiohub.org/public/igem/BBa_K1362204/annotation2410844/range2410844/1
type: Range
persistent identity: range2410844
display id: range2410844
version: 1
direction: direction/reverse
top level: BBa_K1362204
start: 1143
end: 1154
orientation: inline

BBa_K1362447
type: SequenceAnnotation
persistent identity: annotation2410824
display id: annotation2410824
version: 1
top level: BBa_K1362204
location: https://synbiohub.org/public/igem/BBa_K1362204/annotation2410824/range2410824/1
component: BBa_K1362447

https://synbiohub.org/public/igem/BBa_K1362204/annotation2410824/range2410824/1
type: Range
persistent identity: range2410824
display id: range2410824
version: 1
direction: direction/reverse
top level: BBa_K1362204
start: 61
end: 66
orientation: inline

BBa_J70594
type: SequenceAnnotation
persistent identity: annotation2410846
display id: annotation2410846
version: 1
top level: BBa_K1362204
location: https://synbiohub.org/public/igem/BBa_K1362204/annotation2410846/range2410846/1
component: BBa_J70594

https://synbiohub.org/public/igem/BBa_K1362204/annotation2410846/range2410846/1
type: Range
persistent identity: range2410846
display id: range2410846
version: 1
direction: direction/reverse
top level: BBa_K1362204
start: 1155
end: 1160
orientation: inline

BBa_K1362445
type: SequenceAnnotation
persistent identity: annotation2410820
display id: annotation2410820
version: 1
top level: BBa_K1362204
location: https://synbiohub.org/public/igem/BBa_K1362204/annotation2410820/range2410820/1
component: BBa_K1362445

https://synbiohub.org/public/igem/BBa_K1362204/annotation2410820/range2410820/1
type: Range
persistent identity: range2410820
display id: range2410820
version: 1
direction: direction/reverse
top level: BBa_K1362204
start: 1
end: 12
orientation: inline

BBa_J04450
type: SequenceAnnotation
persistent identity: annotation2410842
display id: annotation2410842
version: 1
top level: BBa_K1362204
location: https://synbiohub.org/public/igem/BBa_K1362204/annotation2410842/range2410842/1
component: BBa_J04450

https://synbiohub.org/public/igem/BBa_K1362204/annotation2410842/range2410842/1
type: Range
persistent identity: range2410842
display id: range2410842
version: 1
direction: direction/reverse
top level: BBa_K1362204
start: 67
end: 1135
orientation: inline

BBa_K1362458
type: SequenceAnnotation
persistent identity: annotation2410821
display id: annotation2410821
version: 1
top level: BBa_K1362204
location: https://synbiohub.org/public/igem/BBa_K1362204/annotation2410821/range2410821/1
component: BBa_K1362458

https://synbiohub.org/public/igem/BBa_K1362204/annotation2410821/range2410821/1
type: Range
persistent identity: range2410821
display id: range2410821
version: 1
direction: direction/reverse
top level: BBa_K1362204
start: 13
end: 30
orientation: inline

BBa_K1362453
type: SequenceAnnotation
persistent identity: annotation2410823
display id: annotation2410823
version: 1
top level: BBa_K1362204
location: https://synbiohub.org/public/igem/BBa_K1362204/annotation2410823/range2410823/1
component: BBa_K1362453

https://synbiohub.org/public/igem/BBa_K1362204/annotation2410823/range2410823/1
type: Range
persistent identity: range2410823
display id: range2410823
version: 1
direction: direction/reverse
top level: BBa_K1362204
start: 40
end: 60
orientation: inline

BBa_K1362424
type: SequenceAnnotation
persistent identity: annotation2410843
display id: annotation2410843
version: 1
top level: BBa_K1362204
location: https://synbiohub.org/public/igem/BBa_K1362204/annotation2410843/range2410843/1
component: BBa_K1362424

https://synbiohub.org/public/igem/BBa_K1362204/annotation2410843/range2410843/1
type: Range
persistent identity: range2410843
display id: range2410843
version: 1
direction: direction/reverse
top level: BBa_K1362204
start: 1136
end: 1142
orientation: inline

BBa_K1362449
type: SequenceAnnotation
persistent identity: annotation2410822
display id: annotation2410822
version: 1
top level: BBa_K1362204
location: https://synbiohub.org/public/igem/BBa_K1362204/annotation2410822/range2410822/1
component: BBa_K1362449

https://synbiohub.org/public/igem/BBa_K1362204/annotation2410822/range2410822/1
type: Range
persistent identity: range2410822
display id: range2410822
version: 1
direction: direction/reverse
top level: BBa_K1362204
start: 31
end: 39
orientation: inline

BBa_K1424000
type: ComponentDefinition
persistent identity: BBa K1424000
display id: BBa_K1424000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Prefix homologous recombination for Synechocystis ("Left flank")
creator: Jacob Lamb
created: 2014-09-24T11:00:00Z
modified: 2015-05-08T01:10:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1802_
m user id: 0
owner id: 20667
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is a prefix region of DNA for homologous recombination into a neutral site within the genome of Synechocystis sp. PCC 6803. Kunert, A., Hagemann, M. & Erdmann, N. (2000) Construction of promoter probe vectors of Synechocystis sp. PCC 6803 using the light-emitting reporter systems Gfp and LuxAB. J. Microbiol. Methods. 41, 185-194 Angermayr, S. A., Paszota, M. & Hellingwerf, K. J. (2012) Engineering a cyanobacterial cell factory for production of lactic acid. Appl. Environ. Microbiol. 78, 7098-7106
mutable notes: n/a
mutable provenance: This part comes from genomic sequence of Synechocystis sp. PCC 6803
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence annotation: Synechocystis prefix homologous integration region
sequence: https://synbiohub.org/public/igem/BBa_K1424000_sequence/1

Synechocystis prefix homologous integration region
type: SequenceAnnotation
persistent identity: annotation2400751
display id: annotation2400751
version: 1
top level: BBa_K1424000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1424000/annotation2400751/range2400751/1

https://synbiohub.org/public/igem/BBa_K1424000/annotation2400751/range2400751/1
type: Range
persistent identity: range2400751
display id: range2400751
version: 1
direction: direction/forward
top level: BBa_K1424000
start: 1
end: 556
orientation: inline

BBa_K1689018
type: ComponentDefinition
persistent identity: BBa K1689018
display id: BBa_K1689018
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: dCas9-F3
creator: Wei weijia
created: 2015-09-15T11:00:00Z
modified: 2015-09-27T09:51:28Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2107_
m user id: 26531
owner id: 26531
owning group id: 9
status: status/Planning
bookmark: false
mutable description: pSB1C3-6H-dCas9-F3, this is the fusion protein of dCas9-F3, His tag allows for protein purification
mutable notes: The coding sequence of this fusion protein is too long, you'd better amplify is separately.
mutable provenance: E.coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: dCas9-F3
sequence annotation: start
sequence annotation: F3
sequence annotation: dCas9
sequence annotation: stop
sequence annotation: random spacer
sequence annotation: GGGGS*2 Linker
sequence annotation: His
sequence: https://synbiohub.org/public/igem/BBa_K1689018_sequence/1

dCas9-F3
type: SequenceAnnotation
persistent identity: annotation2469985
display id: annotation2469985
version: 1
top level: BBa_K1689018
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1689018/annotation2469985/range2469985/1

https://synbiohub.org/public/igem/BBa_K1689018/annotation2469985/range2469985/1
type: Range
persistent identity: range2469985
display id: range2469985
version: 1
direction: direction/forward
top level: BBa_K1689018
start: 34
end: 4401
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2477794
display id: annotation2477794
version: 1
top level: BBa_K1689018
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1689018/annotation2477794/range2477794/1

https://synbiohub.org/public/igem/BBa_K1689018/annotation2477794/range2477794/1
type: Range
persistent identity: range2477794
display id: range2477794
version: 1
direction: direction/forward
top level: BBa_K1689018
start: 34
end: 36
orientation: inline

F3
type: SequenceAnnotation
persistent identity: annotation2469996
display id: annotation2469996
version: 1
top level: BBa_K1689018
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1689018/annotation2469996/range2469996/1

https://synbiohub.org/public/igem/BBa_K1689018/annotation2469996/range2469996/1
type: Range
persistent identity: range2469996
display id: range2469996
version: 1
direction: direction/forward
top level: BBa_K1689018
start: 4186
end: 4398
orientation: inline

dCas9
type: SequenceAnnotation
persistent identity: annotation2469992
display id: annotation2469992
version: 1
top level: BBa_K1689018
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1689018/annotation2469992/range2469992/1

https://synbiohub.org/public/igem/BBa_K1689018/annotation2469992/range2469992/1
type: Range
persistent identity: range2469992
display id: range2469992
version: 1
direction: direction/forward
top level: BBa_K1689018
start: 55
end: 4155
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2477795
display id: annotation2477795
version: 1
top level: BBa_K1689018
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1689018/annotation2477795/range2477795/1

https://synbiohub.org/public/igem/BBa_K1689018/annotation2477795/range2477795/1
type: Range
persistent identity: range2477795
display id: range2477795
version: 1
direction: direction/forward
top level: BBa_K1689018
start: 4399
end: 4401
orientation: inline

random spacer
type: SequenceAnnotation
persistent identity: annotation2477796
display id: annotation2477796
version: 1
top level: BBa_K1689018
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1689018/annotation2477796/range2477796/1

https://synbiohub.org/public/igem/BBa_K1689018/annotation2477796/range2477796/1
type: Range
persistent identity: range2477796
display id: range2477796
version: 1
direction: direction/forward
top level: BBa_K1689018
start: 4402
end: 4472
orientation: inline

GGGGS*2 Linker
type: SequenceAnnotation
persistent identity: annotation2469994
display id: annotation2469994
version: 1
top level: BBa_K1689018
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1689018/annotation2469994/range2469994/1

https://synbiohub.org/public/igem/BBa_K1689018/annotation2469994/range2469994/1
type: Range
persistent identity: range2469994
display id: range2469994
version: 1
direction: direction/forward
top level: BBa_K1689018
start: 4156
end: 4185
orientation: inline

His
type: SequenceAnnotation
persistent identity: annotation2469989
display id: annotation2469989
version: 1
top level: BBa_K1689018
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1689018/annotation2469989/range2469989/1

https://synbiohub.org/public/igem/BBa_K1689018/annotation2469989/range2469989/1
type: Range
persistent identity: range2469989
display id: range2469989
version: 1
direction: direction/forward
top level: BBa_K1689018
start: 37
end: 54
orientation: inline

random spacer
type: SequenceAnnotation
persistent identity: annotation2477793
display id: annotation2477793
version: 1
top level: BBa_K1689018
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1689018/annotation2477793/range2477793/1

https://synbiohub.org/public/igem/BBa_K1689018/annotation2477793/range2477793/1
type: Range
persistent identity: range2477793
display id: range2477793
version: 1
direction: direction/forward
top level: BBa_K1689018
start: 1
end: 33
orientation: inline

REDOX
type: ComponentDefinition
persistent identity: BBa K1312006
display id: BBa_K1312006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NADPH-dependent flavinoid reductase relate to Met-DMSP byosynthesis pathway(REDOX)
creator: Yutaka Murata
created: 2014-10-04T11:00:00Z
modified: 2015-05-08T01:09:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1687_
m user id: 0
owner id: 17633
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part is NADPH-dependent flavinoid reductase gene of Fragilariopsis cylindrus. The gene code NADPH-dependent flavinoid reductase relate to Met-DMSP byosynthesis pathway from Fragilariopsis cylindrus. which catalyze second step of Met-DMSP biosynthesis pathway.
mutable notes: This part was excluded intron.
mutable provenance: From Fragilariopsis cylindrus.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: REDOX
sequence: https://synbiohub.org/public/igem/BBa_K1312006_sequence/1

REDOX
type: SequenceAnnotation
persistent identity: annotation2429347
display id: annotation2429347
version: 1
top level: REDOX
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1312006/annotation2429347/range2429347/1

https://synbiohub.org/public/igem/BBa_K1312006/annotation2429347/range2429347/1
type: Range
persistent identity: range2429347
display id: range2429347
version: 1
direction: direction/forward
top level: REDOX
start: 1
end: 705
orientation: inline

BBa_K1362173
type: ComponentDefinition
persistent identity: BBa K1362173
display id: BBa_K1362173
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Split sfGFP reconstitution N-terminal splicing half
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Buescher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schaefer, Carolin Schmelas, Silvan Schmitz, Max Waldhauer
created: 2014-10-09T11:00:00Z
modified: 2015-05-08T01:10:05Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1738_
m user id: 0
owner id: 22830
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_K1362475
component: BBa_K1362471
component: BBa_J70594
component: BBa_K1362400
component: BBa_G0000
component: BBa_J18909
component: BBa_K1362090
sequence annotation: BBa_J70594
sequence annotation: BBa_K1362090
sequence annotation: BBa_K1362400
sequence annotation: BBa_K1362471
sequence annotation: BBa_G0000
sequence annotation: BBa_J18909
sequence annotation: BBa_K1362475
sequence: https://synbiohub.org/public/igem/BBa_K1362173_sequence/1

BBa_K1362475
type: Component
persistent identity: component2414350
display id: component2414350
version: 1
top level: BBa_K1362173
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362475/1

BBa_K1362471
type: Component
persistent identity: component2414349
display id: component2414349
version: 1
top level: BBa_K1362173
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362471/1

BBa_J70594
type: Component
persistent identity: component2414355
display id: component2414355
version: 1
top level: BBa_K1362173
access: public
definition: https://synbiohub.org/public/igem/BBa_J70594/1

BBa_K1362400
type: Component
persistent identity: component2414351
display id: component2414351
version: 1
top level: BBa_K1362173
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362400/1

BBa_G0000
type: Component
persistent identity: component2414345
display id: component2414345
version: 1
top level: BBa_K1362173
access: public
definition: https://synbiohub.org/public/igem/BBa_G0000/1

BBa_J18909
type: Component
persistent identity: component2414353
display id: component2414353
version: 1
top level: BBa_K1362173
access: public
definition: https://synbiohub.org/public/igem/BBa_J18909/1

BBa_K1362090
type: Component
persistent identity: component2414344
display id: component2414344
version: 1
top level: BBa_K1362173
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362090/1

BBa_J70594
type: SequenceAnnotation
persistent identity: annotation2414355
display id: annotation2414355
version: 1
top level: BBa_K1362173
location: https://synbiohub.org/public/igem/BBa_K1362173/annotation2414355/range2414355/1
component: BBa_J70594

https://synbiohub.org/public/igem/BBa_K1362173/annotation2414355/range2414355/1
type: Range
persistent identity: range2414355
display id: range2414355
version: 1
direction: direction/reverse
top level: BBa_K1362173
start: 570
end: 575
orientation: inline

BBa_K1362090
type: SequenceAnnotation
persistent identity: annotation2414344
display id: annotation2414344
version: 1
top level: BBa_K1362173
location: https://synbiohub.org/public/igem/BBa_K1362173/annotation2414344/range2414344/1
component: BBa_K1362090

https://synbiohub.org/public/igem/BBa_K1362173/annotation2414344/range2414344/1
type: Range
persistent identity: range2414344
display id: range2414344
version: 1
direction: direction/reverse
top level: BBa_K1362173
start: 1
end: 29
orientation: inline

BBa_K1362400
type: SequenceAnnotation
persistent identity: annotation2414351
display id: annotation2414351
version: 1
top level: BBa_K1362173
location: https://synbiohub.org/public/igem/BBa_K1362173/annotation2414351/range2414351/1
component: BBa_K1362400

https://synbiohub.org/public/igem/BBa_K1362173/annotation2414351/range2414351/1
type: Range
persistent identity: range2414351
display id: range2414351
version: 1
direction: direction/reverse
top level: BBa_K1362173
start: 250
end: 551
orientation: inline

BBa_K1362471
type: SequenceAnnotation
persistent identity: annotation2414349
display id: annotation2414349
version: 1
top level: BBa_K1362173
location: https://synbiohub.org/public/igem/BBa_K1362173/annotation2414349/range2414349/1
component: BBa_K1362471

https://synbiohub.org/public/igem/BBa_K1362173/annotation2414349/range2414349/1
type: Range
persistent identity: range2414349
display id: range2414349
version: 1
direction: direction/reverse
top level: BBa_K1362173
start: 36
end: 245
orientation: inline

BBa_G0000
type: SequenceAnnotation
persistent identity: annotation2414345
display id: annotation2414345
version: 1
top level: BBa_K1362173
location: https://synbiohub.org/public/igem/BBa_K1362173/annotation2414345/range2414345/1
component: BBa_G0000

https://synbiohub.org/public/igem/BBa_K1362173/annotation2414345/range2414345/1
type: Range
persistent identity: range2414345
display id: range2414345
version: 1
direction: direction/reverse
top level: BBa_K1362173
start: 30
end: 35
orientation: inline

BBa_J18909
type: SequenceAnnotation
persistent identity: annotation2414353
display id: annotation2414353
version: 1
top level: BBa_K1362173
location: https://synbiohub.org/public/igem/BBa_K1362173/annotation2414353/range2414353/1
component: BBa_J18909

https://synbiohub.org/public/igem/BBa_K1362173/annotation2414353/range2414353/1
type: Range
persistent identity: range2414353
display id: range2414353
version: 1
direction: direction/reverse
top level: BBa_K1362173
start: 552
end: 569
orientation: inline

BBa_K1362475
type: SequenceAnnotation
persistent identity: annotation2414350
display id: annotation2414350
version: 1
top level: BBa_K1362173
location: https://synbiohub.org/public/igem/BBa_K1362173/annotation2414350/range2414350/1
component: BBa_K1362475

https://synbiohub.org/public/igem/BBa_K1362173/annotation2414350/range2414350/1
type: Range
persistent identity: range2414350
display id: range2414350
version: 1
direction: direction/reverse
top level: BBa_K1362173
start: 246
end: 249
orientation: inline

BBa_K1758375
type: ComponentDefinition
persistent identity: BBa K1758375
display id: BBa_K1758375
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pblc in front of mRFP+terminator
creator: Team Bielefeld-CeBiTec 2015
created: 2015-08-26T11:00:00Z
modified: 2015-09-18T04:37:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2180_
m user id: 27935
owner id: 26563
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Pblc is the promoter of the blcABC operon from ''Agrobacterium tumefaciens''. It contains operator sites that are recognized by the repressor [http://parts.igem.org/Part:BBa_K1758370 BlcR]. Pblc was described by [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4083662/ Pan et al. 2013].
mutable notes: Pblc was described by [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4083662/ Pan et al. 2013].
mutable provenance: Pblc was cloned in front of [http://parts.igem.org/Part:BBa_K516030 BBa_K516030] in pSB1C3 with Gibson primers.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence annotation: BBa_E1010
sequence annotation: Pblc
sequence annotation: BBa_B0015
sequence annotation: mRFP
sequence: https://synbiohub.org/public/igem/BBa_K1758375_sequence/1

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2438936
display id: annotation2438936
version: 1
top level: BBa_K1758375
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1758375/annotation2438936/range2438936/1

https://synbiohub.org/public/igem/BBa_K1758375/annotation2438936/range2438936/1
type: Range
persistent identity: range2438936
display id: range2438936
version: 1
direction: direction/forward
top level: BBa_K1758375
start: 94
end: 799
orientation: inline

Pblc
type: SequenceAnnotation
persistent identity: annotation2438935
display id: annotation2438935
version: 1
top level: BBa_K1758375
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1758375/annotation2438935/range2438935/1

https://synbiohub.org/public/igem/BBa_K1758375/annotation2438935/range2438935/1
type: Range
persistent identity: range2438935
display id: range2438935
version: 1
direction: direction/forward
top level: BBa_K1758375
start: 1
end: 93
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2438961
display id: annotation2438961
version: 1
top level: BBa_K1758375
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1758375/annotation2438961/range2438961/1

https://synbiohub.org/public/igem/BBa_K1758375/annotation2438961/range2438961/1
type: Range
persistent identity: range2438961
display id: range2438961
version: 1
direction: direction/forward
top level: BBa_K1758375
start: 808
end: 936
orientation: inline

mRFP
type: SequenceAnnotation
persistent identity: annotation2438937
display id: annotation2438937
version: 1
top level: BBa_K1758375
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1758375/annotation2438937/range2438937/1

https://synbiohub.org/public/igem/BBa_K1758375/annotation2438937/range2438937/1
type: Range
persistent identity: range2438937
display id: range2438937
version: 1
direction: direction/forward
top level: BBa_K1758375
start: 94
end: 768
orientation: inline

BBa_K1602016
type: ComponentDefinition
persistent identity: BBa K1602016
display id: BBa_K1602016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0034-xylB-B0034-xylC
creator: Leon Kraus, Jonathan Pletzer-Zelgert, Jonas Sindlinger, Carmen Sakas Gandullo, Sebastian Holzner, Elena Tonchevska, Mirjam Haun
created: 2015-09-15T11:00:00Z
modified: 2015-09-25T01:30:41Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2019_
m user id: 12512
owner id: 22022
owning group id: 9
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1602009
component: BBa_B0034
component: BBa_K1602010
sequence annotation: BBa_K1602010
sequence annotation: BBa_B0034
sequence annotation: BBa_K1602009
sequence: https://synbiohub.org/public/igem/BBa_K1602016_sequence/1

BBa_K1602009
type: Component
persistent identity: component2463075
display id: component2463075
version: 1
top level: BBa_K1602016
access: public
definition: BBa_K1602009

BBa_B0034
type: Component
persistent identity: component2463077
display id: component2463077
version: 1
top level: BBa_K1602016
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: Component
persistent identity: component2463074
display id: component2463074
version: 1
top level: BBa_K1602016
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1602010
type: Component
persistent identity: component2463078
display id: component2463078
version: 1
top level: BBa_K1602016
access: public
definition: BBa_K1602010

BBa_K1602010
type: SequenceAnnotation
persistent identity: annotation2463078
display id: annotation2463078
version: 1
top level: BBa_K1602016
location: https://synbiohub.org/public/igem/BBa_K1602016/annotation2463078/range2463078/1
component: BBa_K1602010

https://synbiohub.org/public/igem/BBa_K1602016/annotation2463078/range2463078/1
type: Range
persistent identity: range2463078
display id: range2463078
version: 1
direction: direction/reverse
top level: BBa_K1602016
start: 792
end: 1661
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2463077
display id: annotation2463077
version: 1
top level: BBa_K1602016
location: https://synbiohub.org/public/igem/BBa_K1602016/annotation2463077/range2463077/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1602016/annotation2463077/range2463077/1
type: Range
persistent identity: range2463077
display id: range2463077
version: 1
direction: direction/reverse
top level: BBa_K1602016
start: 774
end: 785
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2463074
display id: annotation2463074
version: 1
top level: BBa_K1602016
location: https://synbiohub.org/public/igem/BBa_K1602016/annotation2463074/range2463074/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1602016/annotation2463074/range2463074/1
type: Range
persistent identity: range2463074
display id: range2463074
version: 1
direction: direction/reverse
top level: BBa_K1602016
start: 1
end: 12
orientation: inline

BBa_K1602009
type: SequenceAnnotation
persistent identity: annotation2463075
display id: annotation2463075
version: 1
top level: BBa_K1602016
location: https://synbiohub.org/public/igem/BBa_K1602016/annotation2463075/range2463075/1
component: BBa_K1602009

https://synbiohub.org/public/igem/BBa_K1602016/annotation2463075/range2463075/1
type: Range
persistent identity: range2463075
display id: range2463075
version: 1
direction: direction/reverse
top level: BBa_K1602016
start: 19
end: 765
orientation: inline

BBa_K1434001
type: ComponentDefinition
persistent identity: BBa K1434001
display id: BBa_K1434001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: glmS riboswitch
creator: Greta Jasulaityte
created: 2014-09-14T11:00:00Z
modified: 2015-05-08T01:10:25Z
discontinued: false
dominant: false
group u list: _1812_
m user id: 0
owner id: 20624
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: The glmS riboswitch has a self-cleavage activity through which it regulates gene expression.
mutable notes: No
mutable provenance: GenBank
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1434001_sequence/1

BBa_K1699003
type: ComponentDefinition
persistent identity: BBa K1699003
display id: BBa_K1699003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: gRNA for dCas9-VP64 targeting synthetic activation promoter pMLPm
creator: Emil Ruvinov
created: 2015-08-08T11:00:00Z
modified: 2015-08-09T09:45:01Z
discontinued: false
dominant: false
group u list: _2117_
m user id: 24826
owner id: 24826
owning group id: 9
status: status/Planning
bookmark: false
mutable description: gRNA for dCas9-VP64 targeting synthetic activation promoter pMLPm
mutable notes: Design
mutable provenance: Source
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: gRNA for pMLPm
sequence annotation: Hammerhead ribozyme
sequence annotation: gRNA scaffold for SpCas9
sequence annotation: HDV ribozyme
sequence: https://synbiohub.org/public/igem/BBa_K1699003_sequence/1

gRNA for pMLPm
type: SequenceAnnotation
persistent identity: annotation2434600
display id: annotation2434600
version: 1
top level: BBa_K1699003
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1699003/annotation2434600/range2434600/1

https://synbiohub.org/public/igem/BBa_K1699003/annotation2434600/range2434600/1
type: Range
persistent identity: range2434600
display id: range2434600
version: 1
direction: direction/forward
top level: BBa_K1699003
start: 44
end: 63
orientation: inline

Hammerhead ribozyme
type: SequenceAnnotation
persistent identity: annotation2434599
display id: annotation2434599
version: 1
top level: BBa_K1699003
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1699003/annotation2434599/range2434599/1

https://synbiohub.org/public/igem/BBa_K1699003/annotation2434599/range2434599/1
type: Range
persistent identity: range2434599
display id: range2434599
version: 1
direction: direction/forward
top level: BBa_K1699003
start: 1
end: 43
orientation: inline

gRNA scaffold for SpCas9
type: SequenceAnnotation
persistent identity: annotation2434601
display id: annotation2434601
version: 1
top level: BBa_K1699003
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1699003/annotation2434601/range2434601/1

https://synbiohub.org/public/igem/BBa_K1699003/annotation2434601/range2434601/1
type: Range
persistent identity: range2434601
display id: range2434601
version: 1
direction: direction/forward
top level: BBa_K1699003
start: 64
end: 144
orientation: inline

HDV ribozyme
type: SequenceAnnotation
persistent identity: annotation2434602
display id: annotation2434602
version: 1
top level: BBa_K1699003
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1699003/annotation2434602/range2434602/1

https://synbiohub.org/public/igem/BBa_K1699003/annotation2434602/range2434602/1
type: Range
persistent identity: range2434602
display id: range2434602
version: 1
direction: direction/forward
top level: BBa_K1699003
start: 145
end: 210
orientation: inline

BBa_K1659301
type: ComponentDefinition
persistent identity: BBa K1659301
display id: BBa_K1659301
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Micrococcal Nuclease (DNase) fused at N-terminal with DsbA signal sequence
creator: Wei Chung Kong
created: 2015-08-27T11:00:00Z
modified: 2015-09-14T08:14:39Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2077_
m user id: 24524
owner id: 24524
owning group id: 9
status: status/Planning
bookmark: false
mutable description: to be updated
mutable notes: to be updated
mutable provenance: to be updated
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: Hisx6
sequence annotation: start
sequence annotation: DsbA 2-19 signal sequence
sequence annotation: stop
sequence annotation: Micrococcal DNase
sequence: https://synbiohub.org/public/igem/BBa_K1659301_sequence/1

Hisx6
type: SequenceAnnotation
persistent identity: annotation2449092
display id: annotation2449092
version: 1
top level: BBa_K1659301
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1659301/annotation2449092/range2449092/1

https://synbiohub.org/public/igem/BBa_K1659301/annotation2449092/range2449092/1
type: Range
persistent identity: range2449092
display id: range2449092
version: 1
direction: direction/forward
top level: BBa_K1659301
start: 559
end: 576
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2449089
display id: annotation2449089
version: 1
top level: BBa_K1659301
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1659301/annotation2449089/range2449089/1

https://synbiohub.org/public/igem/BBa_K1659301/annotation2449089/range2449089/1
type: Range
persistent identity: range2449089
display id: range2449089
version: 1
direction: direction/forward
top level: BBa_K1659301
start: 1
end: 3
orientation: inline

DsbA 2-19 signal sequence
type: SequenceAnnotation
persistent identity: annotation2449090
display id: annotation2449090
version: 1
top level: BBa_K1659301
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1659301/annotation2449090/range2449090/1

https://synbiohub.org/public/igem/BBa_K1659301/annotation2449090/range2449090/1
type: Range
persistent identity: range2449090
display id: range2449090
version: 1
direction: direction/forward
top level: BBa_K1659301
start: 4
end: 57
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2449093
display id: annotation2449093
version: 1
top level: BBa_K1659301
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1659301/annotation2449093/range2449093/1

https://synbiohub.org/public/igem/BBa_K1659301/annotation2449093/range2449093/1
type: Range
persistent identity: range2449093
display id: range2449093
version: 1
direction: direction/forward
top level: BBa_K1659301
start: 577
end: 582
orientation: inline

Micrococcal DNase
type: SequenceAnnotation
persistent identity: annotation2449091
display id: annotation2449091
version: 1
top level: BBa_K1659301
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1659301/annotation2449091/range2449091/1

https://synbiohub.org/public/igem/BBa_K1659301/annotation2449091/range2449091/1
type: Range
persistent identity: range2449091
display id: range2449091
version: 1
direction: direction/forward
top level: BBa_K1659301
start: 58
end: 558
orientation: inline

BBa_K1321344
type: ComponentDefinition
persistent identity: BBa K1321344
display id: BBa_K1321344
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NiBP fused to CBDcex in RFC 25
creator: Chris N Micklem
created: 2014-10-07T11:00:00Z
modified: 2015-06-17T12:26:09Z
discontinued: false
dominant: false
group u list: _1696_
m user id: 4206
owner id: 20830
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: asdf
mutable notes: asdf
mutable provenance: asdf
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_B0105
component: BBa_K1321003
component: BBa_K1321009
sequence annotation: BBa_K1321009
sequence annotation: BBa_K1321003
sequence annotation: BBa_B0105
sequence: https://synbiohub.org/public/igem/BBa_K1321344_sequence/1

BBa_B0105
type: Component
persistent identity: component2415106
display id: component2415106
version: 1
top level: BBa_K1321344
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_K1321003
type: Component
persistent identity: component2415107
display id: component2415107
version: 1
top level: BBa_K1321344
access: public
definition: https://synbiohub.org/public/igem/BBa_K1321003/1

BBa_K1321009
type: Component
persistent identity: component2415104
display id: component2415104
version: 1
top level: BBa_K1321344
access: public
definition: BBa_K1321009

BBa_K1321009
type: SequenceAnnotation
persistent identity: annotation2415104
display id: annotation2415104
version: 1
top level: BBa_K1321344
location: https://synbiohub.org/public/igem/BBa_K1321344/annotation2415104/range2415104/1
component: BBa_K1321009

https://synbiohub.org/public/igem/BBa_K1321344/annotation2415104/range2415104/1
type: Range
persistent identity: range2415104
display id: range2415104
version: 1
direction: direction/reverse
top level: BBa_K1321344
start: 1
end: 176
orientation: inline

BBa_K1321003
type: SequenceAnnotation
persistent identity: annotation2415107
display id: annotation2415107
version: 1
top level: BBa_K1321344
location: https://synbiohub.org/public/igem/BBa_K1321344/annotation2415107/range2415107/1
component: BBa_K1321003

https://synbiohub.org/public/igem/BBa_K1321344/annotation2415107/range2415107/1
type: Range
persistent identity: range2415107
display id: range2415107
version: 1
direction: direction/reverse
top level: BBa_K1321344
start: 183
end: 509
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2415106
display id: annotation2415106
version: 1
top level: BBa_K1321344
location: https://synbiohub.org/public/igem/BBa_K1321344/annotation2415106/range2415106/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_K1321344/annotation2415106/range2415106/1
type: Range
persistent identity: range2415106
display id: range2415106
version: 1
direction: direction/reverse
top level: BBa_K1321344
start: 177
end: 182
orientation: inline

BBa_K1763012
type: ComponentDefinition
persistent identity: BBa K1763012
display id: BBa_K1763012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Major Spidroin Protein 1 (MaSp1) with Sticky Ends CA
creator: Vinson Lam
created: 2015-07-26T11:00:00Z
modified: 2015-09-18T11:29:29Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2185_
m user id: 27136
owner id: 27136
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This part contains the core sequence of MaSp1 that has been assembled with a 5'-AGTT-3' overhang on the 5' end of the sequence and 3'-ACAG-5' overhang on the 3' end of the sequence. This part was designed for use with ICA, along with parts BBa_K1763003 and BBa_K1763004 as an efficient method to assemble multiple MaSp sequences together. This setup is to show that this particular set of sticky ends is suitable for ICA. Before using in ICA, this part should be digested with BsaI, a type IIs restriction enzyme, which cuts DNA outside of its recognition site.
mutable notes: The gene sequence for MaSp2 is particularly GC-rich, which may complicate PCR.
mutable provenance: This part is derived from the MaSp2 gene found in the spider N. Clavipes.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: BsaI recognition site (forward)
sequence annotation: C sticky end
sequence annotation: MaSp1 Core
sequence annotation: A sticky end
sequence annotation: BsaI recognition site (reverse)
sequence: https://synbiohub.org/public/igem/BBa_K1763012_sequence/1

BsaI recognition site (forward)
type: SequenceAnnotation
persistent identity: annotation2433556
display id: annotation2433556
version: 1
top level: BBa_K1763012
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1763012/annotation2433556/range2433556/1

https://synbiohub.org/public/igem/BBa_K1763012/annotation2433556/range2433556/1
type: Range
persistent identity: range2433556
display id: range2433556
version: 1
direction: direction/forward
top level: BBa_K1763012
start: 1
end: 5
orientation: inline

C sticky end
type: SequenceAnnotation
persistent identity: annotation2463777
display id: annotation2463777
version: 1
top level: BBa_K1763012
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1763012/annotation2463777/range2463777/1

https://synbiohub.org/public/igem/BBa_K1763012/annotation2463777/range2463777/1
type: Range
persistent identity: range2463777
display id: range2463777
version: 1
direction: direction/forward
top level: BBa_K1763012
start: 7
end: 10
orientation: inline

MaSp1 Core
type: SequenceAnnotation
persistent identity: annotation2473425
display id: annotation2473425
version: 1
top level: BBa_K1763012
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763012/annotation2473425/range2473425/1

https://synbiohub.org/public/igem/BBa_K1763012/annotation2473425/range2473425/1
type: Range
persistent identity: range2473425
display id: range2473425
version: 1
direction: direction/forward
top level: BBa_K1763012
start: 11
end: 108
orientation: inline

A sticky end
type: SequenceAnnotation
persistent identity: annotation2463778
display id: annotation2463778
version: 1
top level: BBa_K1763012
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1763012/annotation2463778/range2463778/1

https://synbiohub.org/public/igem/BBa_K1763012/annotation2463778/range2463778/1
type: Range
persistent identity: range2463778
display id: range2463778
version: 1
direction: direction/forward
top level: BBa_K1763012
start: 109
end: 112
orientation: inline

BsaI recognition site (reverse)
type: SequenceAnnotation
persistent identity: annotation2433557
display id: annotation2433557
version: 1
top level: BBa_K1763012
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1763012/annotation2433557/range2433557/1

https://synbiohub.org/public/igem/BBa_K1763012/annotation2433557/range2433557/1
type: Range
persistent identity: range2433557
display id: range2433557
version: 1
direction: direction/reverse
top level: BBa_K1763012
start: 114
end: 119
orientation: inline

BBa_K1491018
type: ComponentDefinition
persistent identity: BBa K1491018
display id: BBa_K1491018
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lac Inducible Codon Optimized Supernova
creator: Danielle Peters
created: 2014-08-03T11:00:00Z
modified: 2015-05-08T01:10:44Z
discontinued: false
dominant: false
group u list: _1871_
m user id: 0
owner id: 22521
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Supernova is the monomeric version of KillerRed (BB_K1184000). Codon optimized Supernova with WTLac promoter allows for induction of Supernova expression with IPTG.
mutable notes: Lac inducible Supernova
mutable provenance: The original KillerRed is BBa_K1184000
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1491017
component: BBa_J04500
sequence annotation: BBa_K1491017
sequence annotation: BBa_J04500
sequence: https://synbiohub.org/public/igem/BBa_K1491018_sequence/1

BBa_K1491017
type: Component
persistent identity: component2380367
display id: component2380367
version: 1
top level: BBa_K1491018
access: public
definition: BBa_K1491017

BBa_J04500
type: Component
persistent identity: component2380365
display id: component2380365
version: 1
top level: BBa_K1491018
access: public
definition: https://synbiohub.org/public/igem/BBa_J04500/1

BBa_K1491017
type: SequenceAnnotation
persistent identity: annotation2380367
display id: annotation2380367
version: 1
top level: BBa_K1491018
location: https://synbiohub.org/public/igem/BBa_K1491018/annotation2380367/range2380367/1
component: BBa_K1491017

https://synbiohub.org/public/igem/BBa_K1491018/annotation2380367/range2380367/1
type: Range
persistent identity: range2380367
display id: range2380367
version: 1
direction: direction/reverse
top level: BBa_K1491018
start: 227
end: 967
orientation: inline

BBa_J04500
type: SequenceAnnotation
persistent identity: annotation2380365
display id: annotation2380365
version: 1
top level: BBa_K1491018
location: https://synbiohub.org/public/igem/BBa_K1491018/annotation2380365/range2380365/1
component: BBa_J04500

https://synbiohub.org/public/igem/BBa_K1491018/annotation2380365/range2380365/1
type: Range
persistent identity: range2380365
display id: range2380365
version: 1
direction: direction/reverse
top level: BBa_K1491018
start: 1
end: 220
orientation: inline

BBa_K1350005
type: ComponentDefinition
persistent identity: BBa K1350005
display id: BBa_K1350005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Mdfa Antiporter
creator: Zhipeng Qiu
created: 2014-09-27T11:00:00Z
modified: 2015-05-08T01:10:01Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1725_
m user id: 0
owner id: 20480
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: MDFA is an antiporter which can make E-coli have a ability to tolerate high alkiline environment up to pH 10.
mutable notes: We use pcr to get the target genes from E-coli and invert them into pet28a which can multicopy.
mutable provenance: Escherchia coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1350005_sequence/1

BBa_K1399012
type: ComponentDefinition
persistent identity: BBa K1399012
display id: BBa_K1399012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Plac-RFP(LAA)
creator: Anna Stikane
created: 2014-09-18T11:00:00Z
modified: 2015-05-08T01:10:16Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1777_
m user id: 0
owner id: 22477
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: Lactose/IPTG inducible promoter with RBS, RFP reporter tagged with LAA-ssrA degradation tag and terminator. The tagged RFP is actively degraded within cell, thus provides better temporal resolution of red fluorescence and promoter activity.
mutable notes: Part was assembled using BrickClip assembly (BBF RFC 104) using other biobrick parts as templates. BrickClip assembly is a special case of more general Paperclip assembly method.[1]
mutable provenance: other biobricks
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: T7 TE
sequence annotation: stem_loop
sequence annotation: barcode from BBa_E1010
sequence annotation: BBa_B0015
sequence annotation: LacI binding site
sequence annotation: BBa_B0010
sequence annotation: BBa_R0010
sequence annotation: -10
sequence annotation: BBa_K1399002
sequence annotation: stop
sequence annotation: CAP binding site
sequence annotation: start
sequence annotation: tag
sequence annotation: conserved
sequence annotation: BBa_B0034
sequence annotation: -35
sequence annotation: RFP
sequence annotation: end of LacI coding region (inactive)
sequence annotation: BBa_B0012
sequence annotation: polya
sequence: https://synbiohub.org/public/igem/BBa_K1399012_sequence/1

T7 TE
type: SequenceAnnotation
persistent identity: annotation2384004
display id: annotation2384004
version: 1
top level: BBa_K1399012
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2384004/range2384004/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2384004/range2384004/1
type: Range
persistent identity: range2384004
display id: range2384004
version: 1
direction: direction/forward
top level: BBa_K1399012
start: 1069
end: 1088
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation2384002
display id: annotation2384002
version: 1
top level: BBa_K1399012
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2384002/range2384002/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2384002/range2384002/1
type: Range
persistent identity: range2384002
display id: range2384002
version: 1
direction: direction/forward
top level: BBa_K1399012
start: 985
end: 1028
orientation: inline

barcode from BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2386312
display id: annotation2386312
version: 1
top level: BBa_K1399012
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2386312/range2386312/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2386312/range2386312/1
type: Range
persistent identity: range2386312
display id: range2386312
version: 1
direction: direction/forward
top level: BBa_K1399012
start: 941
end: 965
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2384007
display id: annotation2384007
version: 1
top level: BBa_K1399012
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2384007/range2384007/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2384007/range2384007/1
type: Range
persistent identity: range2384007
display id: range2384007
version: 1
direction: direction/reverse
top level: BBa_K1399012
start: 974
end: 1102
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation2383991
display id: annotation2383991
version: 1
top level: BBa_K1399012
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2383991/range2383991/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2383991/range2383991/1
type: Range
persistent identity: range2383991
display id: range2383991
version: 1
direction: direction/forward
top level: BBa_K1399012
start: 166
end: 200
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2384001
display id: annotation2384001
version: 1
top level: BBa_K1399012
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2384001/range2384001/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2384001/range2384001/1
type: Range
persistent identity: range2384001
display id: range2384001
version: 1
direction: direction/forward
top level: BBa_K1399012
start: 974
end: 1053
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2383986
display id: annotation2383986
version: 1
top level: BBa_K1399012
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2383986/range2383986/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2383986/range2383986/1
type: Range
persistent identity: range2383986
display id: range2383986
version: 1
direction: direction/forward
top level: BBa_K1399012
start: 1
end: 200
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2383990
display id: annotation2383990
version: 1
top level: BBa_K1399012
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2383990/range2383990/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2383990/range2383990/1
type: Range
persistent identity: range2383990
display id: range2383990
version: 1
direction: direction/forward
top level: BBa_K1399012
start: 161
end: 166
orientation: inline

BBa_K1399002
type: SequenceAnnotation
persistent identity: annotation2384000
display id: annotation2384000
version: 1
top level: BBa_K1399012
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2384000/range2384000/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2384000/range2384000/1
type: Range
persistent identity: range2384000
display id: range2384000
version: 1
direction: direction/reverse
top level: BBa_K1399012
start: 227
end: 940
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2383999
display id: annotation2383999
version: 1
top level: BBa_K1399012
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2383999/range2383999/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2383999/range2383999/1
type: Range
persistent identity: range2383999
display id: range2383999
version: 1
direction: direction/forward
top level: BBa_K1399012
start: 938
end: 940
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation2383988
display id: annotation2383988
version: 1
top level: BBa_K1399012
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2383988/range2383988/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2383988/range2383988/1
type: Range
persistent identity: range2383988
display id: range2383988
version: 1
direction: direction/forward
top level: BBa_K1399012
start: 89
end: 126
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2383992
display id: annotation2383992
version: 1
top level: BBa_K1399012
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2383992/range2383992/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2383992/range2383992/1
type: Range
persistent identity: range2383992
display id: range2383992
version: 1
direction: direction/forward
top level: BBa_K1399012
start: 173
end: 173
orientation: inline

tag
type: SequenceAnnotation
persistent identity: annotation2383997
display id: annotation2383997
version: 1
top level: BBa_K1399012
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2383997/range2383997/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2383997/range2383997/1
type: Range
persistent identity: range2383997
display id: range2383997
version: 1
direction: direction/forward
top level: BBa_K1399012
start: 902
end: 934
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation2383993
display id: annotation2383993
version: 1
top level: BBa_K1399012
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2383993/range2383993/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2383993/range2383993/1
type: Range
persistent identity: range2383993
display id: range2383993
version: 1
direction: direction/forward
top level: BBa_K1399012
start: 213
end: 216
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2383994
display id: annotation2383994
version: 1
top level: BBa_K1399012
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2383994/range2383994/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2383994/range2383994/1
type: Range
persistent identity: range2383994
display id: range2383994
version: 1
direction: direction/reverse
top level: BBa_K1399012
start: 209
end: 220
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2383989
display id: annotation2383989
version: 1
top level: BBa_K1399012
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2383989/range2383989/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2383989/range2383989/1
type: Range
persistent identity: range2383989
display id: range2383989
version: 1
direction: direction/forward
top level: BBa_K1399012
start: 137
end: 142
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2383995
display id: annotation2383995
version: 1
top level: BBa_K1399012
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2383995/range2383995/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2383995/range2383995/1
type: Range
persistent identity: range2383995
display id: range2383995
version: 1
direction: direction/forward
top level: BBa_K1399012
start: 227
end: 229
orientation: inline

RFP
type: SequenceAnnotation
persistent identity: annotation2383996
display id: annotation2383996
version: 1
top level: BBa_K1399012
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2383996/range2383996/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2383996/range2383996/1
type: Range
persistent identity: range2383996
display id: range2383996
version: 1
direction: direction/forward
top level: BBa_K1399012
start: 230
end: 901
orientation: inline

end of LacI coding region (inactive)
type: SequenceAnnotation
persistent identity: annotation2383987
display id: annotation2383987
version: 1
top level: BBa_K1399012
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2383987/range2383987/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2383987/range2383987/1
type: Range
persistent identity: range2383987
display id: range2383987
version: 1
direction: direction/forward
top level: BBa_K1399012
start: 1
end: 88
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2384003
display id: annotation2384003
version: 1
top level: BBa_K1399012
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2384003/range2384003/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2384003/range2384003/1
type: Range
persistent identity: range2384003
display id: range2384003
version: 1
direction: direction/forward
top level: BBa_K1399012
start: 1062
end: 1102
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2384006
display id: annotation2384006
version: 1
top level: BBa_K1399012
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2384006/range2384006/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2384006/range2384006/1
type: Range
persistent identity: range2384006
display id: range2384006
version: 1
direction: direction/forward
top level: BBa_K1399012
start: 1095
end: 1095
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2383998
display id: annotation2383998
version: 1
top level: BBa_K1399012
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2383998/range2383998/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2383998/range2383998/1
type: Range
persistent identity: range2383998
display id: range2383998
version: 1
direction: direction/forward
top level: BBa_K1399012
start: 935
end: 937
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation2384005
display id: annotation2384005
version: 1
top level: BBa_K1399012
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2384005/range2384005/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2384005/range2384005/1
type: Range
persistent identity: range2384005
display id: range2384005
version: 1
direction: direction/forward
top level: BBa_K1399012
start: 1089
end: 1102
orientation: inline

BBa_K1789003
type: ComponentDefinition
persistent identity: BBa K1789003
display id: BBa_K1789003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP1(with termination codon)
creator: Xinyuan Qiu
created: 2015-09-13T11:00:00Z
modified: 2016-01-25T01:04:29Z
discontinued: false
dominant: false
group u list: _2214_
m user id: 4206
owner id: 21134
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This part is the Amino Half of GFP with termination codon
mutable notes: Because of the need of our project, we added a termination codon when designing the PCR.
mutable provenance: This part is gained from part BBa_I715019. we added the termination codon when designing the PCR.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: termination codon
sequence annotation: GFP1
sequence: https://synbiohub.org/public/igem/BBa_K1789003_sequence/1

termination codon
type: SequenceAnnotation
persistent identity: annotation2454066
display id: annotation2454066
version: 1
top level: BBa_K1789003
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1789003/annotation2454066/range2454066/1

https://synbiohub.org/public/igem/BBa_K1789003/annotation2454066/range2454066/1
type: Range
persistent identity: range2454066
display id: range2454066
version: 1
direction: direction/forward
top level: BBa_K1789003
start: 472
end: 474
orientation: inline

GFP1
type: SequenceAnnotation
persistent identity: annotation2454065
display id: annotation2454065
version: 1
top level: BBa_K1789003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1789003/annotation2454065/range2454065/1

https://synbiohub.org/public/igem/BBa_K1789003/annotation2454065/range2454065/1
type: Range
persistent identity: range2454065
display id: range2454065
version: 1
direction: direction/forward
top level: BBa_K1789003
start: 1
end: 471
orientation: inline

BBa_K1523022
type: ComponentDefinition
persistent identity: BBa K1523022
display id: BBa_K1523022
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: nahE coding sequence
creator: Haotian Wang
created: 2014-10-02T11:00:00Z
modified: 2015-05-08T01:10:50Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _1905_
m user id: 0
owner id: 21246
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: The gene encoding trans-o-hydroxybenzylidenepyruvate hydratase-aldolase (tHBP-HA) was isolated from Pseudomonas fluorescens N3, an environmental strain able to degrade naphthalene.
mutable provenance: Pseudomonas fluorescens (strain: PC20, biotype: F) NCBI Gene ID: 7871527
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1523022_sequence/1

BBa_K1399013
type: ComponentDefinition
persistent identity: BBa K1399013
display id: BBa_K1399013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Plac-RFP(DAS)
creator: Anna Stikane
created: 2014-09-18T11:00:00Z
modified: 2015-05-08T01:10:16Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1777_
m user id: 0
owner id: 22477
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: Lactose/IPTG inducible promoter with RFP reporter tagged with DAS-ssrA degradation tag followed by terminator. The tagged RFP is degraded within cell, thus provides better temporal resolution of red fluorescence and promoter activity. Presence of SspB is crucial for effective protein degradation.
mutable notes: Part was assembled using BrickClip assembly (BBF RFC104) using other biobrick parts as templates. BrickClip assembly is a special case of more general Paperclip assembly method.[1]
mutable provenance: other biobricks
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: BBa_R0010
sequence annotation: stop
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: end of LacI coding region (inactive)
sequence annotation: RFP
sequence annotation: barcode from BBa_E1010
sequence annotation: start
sequence annotation: CAP binding site
sequence annotation: polya
sequence annotation: -35
sequence annotation: BBa_B0015
sequence annotation: -10
sequence annotation: LacI binding site
sequence annotation: conserved
sequence annotation: BBa_B0034
sequence annotation: BBa_K1399003
sequence annotation: DAS-ssrA tag
sequence annotation: stem_loop
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_K1399013_sequence/1

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2384012
display id: annotation2384012
version: 1
top level: BBa_K1399013
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384012/range2384012/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384012/range2384012/1
type: Range
persistent identity: range2384012
display id: range2384012
version: 1
direction: direction/forward
top level: BBa_K1399013
start: 1
end: 200
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2384024
display id: annotation2384024
version: 1
top level: BBa_K1399013
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384024/range2384024/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384024/range2384024/1
type: Range
persistent identity: range2384024
display id: range2384024
version: 1
direction: direction/forward
top level: BBa_K1399013
start: 935
end: 937
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2384029
display id: annotation2384029
version: 1
top level: BBa_K1399013
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384029/range2384029/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384029/range2384029/1
type: Range
persistent identity: range2384029
display id: range2384029
version: 1
direction: direction/forward
top level: BBa_K1399013
start: 1062
end: 1102
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation2384030
display id: annotation2384030
version: 1
top level: BBa_K1399013
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384030/range2384030/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384030/range2384030/1
type: Range
persistent identity: range2384030
display id: range2384030
version: 1
direction: direction/forward
top level: BBa_K1399013
start: 1069
end: 1088
orientation: inline

end of LacI coding region (inactive)
type: SequenceAnnotation
persistent identity: annotation2384013
display id: annotation2384013
version: 1
top level: BBa_K1399013
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384013/range2384013/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384013/range2384013/1
type: Range
persistent identity: range2384013
display id: range2384013
version: 1
direction: direction/forward
top level: BBa_K1399013
start: 1
end: 88
orientation: inline

RFP
type: SequenceAnnotation
persistent identity: annotation2384022
display id: annotation2384022
version: 1
top level: BBa_K1399013
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384022/range2384022/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384022/range2384022/1
type: Range
persistent identity: range2384022
display id: range2384022
version: 1
direction: direction/forward
top level: BBa_K1399013
start: 230
end: 901
orientation: inline

barcode from BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2386313
display id: annotation2386313
version: 1
top level: BBa_K1399013
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2386313/range2386313/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2386313/range2386313/1
type: Range
persistent identity: range2386313
display id: range2386313
version: 1
direction: direction/forward
top level: BBa_K1399013
start: 941
end: 965
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2384018
display id: annotation2384018
version: 1
top level: BBa_K1399013
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384018/range2384018/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384018/range2384018/1
type: Range
persistent identity: range2384018
display id: range2384018
version: 1
direction: direction/forward
top level: BBa_K1399013
start: 173
end: 173
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation2384014
display id: annotation2384014
version: 1
top level: BBa_K1399013
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384014/range2384014/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384014/range2384014/1
type: Range
persistent identity: range2384014
display id: range2384014
version: 1
direction: direction/forward
top level: BBa_K1399013
start: 89
end: 126
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation2384031
display id: annotation2384031
version: 1
top level: BBa_K1399013
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384031/range2384031/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384031/range2384031/1
type: Range
persistent identity: range2384031
display id: range2384031
version: 1
direction: direction/forward
top level: BBa_K1399013
start: 1089
end: 1102
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2384015
display id: annotation2384015
version: 1
top level: BBa_K1399013
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384015/range2384015/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384015/range2384015/1
type: Range
persistent identity: range2384015
display id: range2384015
version: 1
direction: direction/forward
top level: BBa_K1399013
start: 137
end: 142
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2384033
display id: annotation2384033
version: 1
top level: BBa_K1399013
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384033/range2384033/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384033/range2384033/1
type: Range
persistent identity: range2384033
display id: range2384033
version: 1
direction: direction/reverse
top level: BBa_K1399013
start: 974
end: 1102
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2384016
display id: annotation2384016
version: 1
top level: BBa_K1399013
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384016/range2384016/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384016/range2384016/1
type: Range
persistent identity: range2384016
display id: range2384016
version: 1
direction: direction/forward
top level: BBa_K1399013
start: 161
end: 166
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation2384017
display id: annotation2384017
version: 1
top level: BBa_K1399013
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384017/range2384017/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384017/range2384017/1
type: Range
persistent identity: range2384017
display id: range2384017
version: 1
direction: direction/forward
top level: BBa_K1399013
start: 166
end: 200
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation2384019
display id: annotation2384019
version: 1
top level: BBa_K1399013
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384019/range2384019/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384019/range2384019/1
type: Range
persistent identity: range2384019
display id: range2384019
version: 1
direction: direction/forward
top level: BBa_K1399013
start: 213
end: 216
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2384020
display id: annotation2384020
version: 1
top level: BBa_K1399013
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384020/range2384020/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384020/range2384020/1
type: Range
persistent identity: range2384020
display id: range2384020
version: 1
direction: direction/reverse
top level: BBa_K1399013
start: 209
end: 220
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2384021
display id: annotation2384021
version: 1
top level: BBa_K1399013
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384021/range2384021/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384021/range2384021/1
type: Range
persistent identity: range2384021
display id: range2384021
version: 1
direction: direction/forward
top level: BBa_K1399013
start: 227
end: 229
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2384025
display id: annotation2384025
version: 1
top level: BBa_K1399013
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384025/range2384025/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384025/range2384025/1
type: Range
persistent identity: range2384025
display id: range2384025
version: 1
direction: direction/forward
top level: BBa_K1399013
start: 938
end: 940
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2384032
display id: annotation2384032
version: 1
top level: BBa_K1399013
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384032/range2384032/1

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type: Range
persistent identity: range2384032
display id: range2384032
version: 1
direction: direction/forward
top level: BBa_K1399013
start: 1095
end: 1095
orientation: inline

BBa_K1399003
type: SequenceAnnotation
persistent identity: annotation2384026
display id: annotation2384026
version: 1
top level: BBa_K1399013
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384026/range2384026/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384026/range2384026/1
type: Range
persistent identity: range2384026
display id: range2384026
version: 1
direction: direction/reverse
top level: BBa_K1399013
start: 227
end: 940
orientation: inline

DAS-ssrA tag
type: SequenceAnnotation
persistent identity: annotation2384023
display id: annotation2384023
version: 1
top level: BBa_K1399013
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384023/range2384023/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384023/range2384023/1
type: Range
persistent identity: range2384023
display id: range2384023
version: 1
direction: direction/forward
top level: BBa_K1399013
start: 902
end: 934
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation2384028
display id: annotation2384028
version: 1
top level: BBa_K1399013
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384028/range2384028/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384028/range2384028/1
type: Range
persistent identity: range2384028
display id: range2384028
version: 1
direction: direction/forward
top level: BBa_K1399013
start: 985
end: 1028
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2384027
display id: annotation2384027
version: 1
top level: BBa_K1399013
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384027/range2384027/1

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type: Range
persistent identity: range2384027
display id: range2384027
version: 1
direction: direction/forward
top level: BBa_K1399013
start: 974
end: 1053
orientation: inline

NAGA
type: ComponentDefinition
persistent identity: BBa K1483000
display id: BBa_K1483000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: α-N-Acetylgalactosamindase
creator: Philipp H.O. Mayer and Philip Roessler
created: 2014-10-05T11:00:00Z
modified: 2015-05-08T01:10:41Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1863_
m user id: 0
owner id: 21259
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Encodes for an enzyme, that is capable of cleaving off N-Acetlygalactosamine from A-group blood antigens, thereby turning them into H-antigens. Can be used to convert erythrocytes from blood type A to O. Part in RFC25 standard.
mutable notes: Part in RFC25, in order to ease fusion with other parts, for example immobilisation tags.
mutable provenance: The protein encoded by this sequence naturally occurs in Elizabethkingia meningoseptica( also called Chryseobacterium meningosepticum or Flavobacterium meningosepticum). This sequence was optimized for codon usage in E. coli K12 and synthesized.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: N-Acetylgalactosaminidase
sequence: https://synbiohub.org/public/igem/BBa_K1483000_sequence/1

N-Acetylgalactosaminidase
type: SequenceAnnotation
persistent identity: annotation2396225
display id: annotation2396225
version: 1
top level: NAGA
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1483000/annotation2396225/range2396225/1

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type: Range
persistent identity: range2396225
display id: range2396225
version: 1
direction: direction/forward
top level: NAGA
start: 1
end: 1329
orientation: inline

BBa_K1640008
type: ComponentDefinition
persistent identity: BBa K1640008
display id: BBa_K1640008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: psbMZH
creator: Meghan Cook
created: 2015-09-09T11:00:00Z
modified: 2015-09-18T08:53:22Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2057_
m user id: 28565
owner id: 28565
owning group id: 9
status: status/Planning
bookmark: true
mutable description: N/a
mutable notes: N/a
mutable provenance: N/a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: psbH
sequence annotation: rbs
sequence annotation: psbZ
sequence annotation: psbM
sequence annotation: Plac
sequence: https://synbiohub.org/public/igem/BBa_K1640008_sequence/1

psbH
type: SequenceAnnotation
persistent identity: annotation2448250
display id: annotation2448250
version: 1
top level: BBa_K1640008
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1640008/annotation2448250/range2448250/1

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type: Range
persistent identity: range2448250
display id: range2448250
version: 1
direction: direction/forward
top level: BBa_K1640008
start: 321
end: 488
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2448249
display id: annotation2448249
version: 1
top level: BBa_K1640008
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1640008/annotation2448249/range2448249/1

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type: Range
persistent identity: range2448249
display id: range2448249
version: 1
direction: direction/forward
top level: BBa_K1640008
start: 308
end: 320
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2448245
display id: annotation2448245
version: 1
top level: BBa_K1640008
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1640008/annotation2448245/range2448245/1

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type: Range
persistent identity: range2448245
display id: range2448245
version: 1
direction: direction/forward
top level: BBa_K1640008
start: 41
end: 57
orientation: inline

psbZ
type: SequenceAnnotation
persistent identity: annotation2448248
display id: annotation2448248
version: 1
top level: BBa_K1640008
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1640008/annotation2448248/range2448248/1

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persistent identity: range2448248
display id: range2448248
version: 1
direction: direction/forward
top level: BBa_K1640008
start: 176
end: 307
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2448247
display id: annotation2448247
version: 1
top level: BBa_K1640008
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1640008/annotation2448247/range2448247/1

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type: Range
persistent identity: range2448247
display id: range2448247
version: 1
direction: direction/forward
top level: BBa_K1640008
start: 163
end: 175
orientation: inline

psbM
type: SequenceAnnotation
persistent identity: annotation2448246
display id: annotation2448246
version: 1
top level: BBa_K1640008
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1640008/annotation2448246/range2448246/1

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type: Range
persistent identity: range2448246
display id: range2448246
version: 1
direction: direction/forward
top level: BBa_K1640008
start: 58
end: 162
orientation: inline

Plac
type: SequenceAnnotation
persistent identity: annotation2448244
display id: annotation2448244
version: 1
top level: BBa_K1640008
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1640008/annotation2448244/range2448244/1

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type: Range
persistent identity: range2448244
display id: range2448244
version: 1
direction: direction/forward
top level: BBa_K1640008
start: 1
end: 40
orientation: inline

BBa_K1689012
type: ComponentDefinition
persistent identity: BBa K1689012
display id: BBa_K1689012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: dCas9-Nlact
creator: Jiaofeng Li
created: 2015-09-15T11:00:00Z
modified: 2015-11-17T09:39:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2107_
m user id: 26531
owner id: 26434
owning group id: 9
status: status/Planning
bookmark: false
mutable description: NFluc of lactamase fused with dCas9, His tag allows for protein purification
mutable notes: goldengate
mutable provenance: E.coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: dCas9
sequence annotation: His
sequence annotation: GGGGS*2 Linker
sequence annotation: stop
sequence annotation: Nlact for N terminal of β-Lactamase
sequence annotation: dCas9-Nlact
sequence annotation: random spacer
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_K1689012_sequence/1

dCas9
type: SequenceAnnotation
persistent identity: annotation2470079
display id: annotation2470079
version: 1
top level: BBa_K1689012
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1689012/annotation2470079/range2470079/1

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type: Range
persistent identity: range2470079
display id: range2470079
version: 1
direction: direction/forward
top level: BBa_K1689012
start: 55
end: 4155
orientation: inline

His
type: SequenceAnnotation
persistent identity: annotation2470058
display id: annotation2470058
version: 1
top level: BBa_K1689012
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1689012/annotation2470058/range2470058/1

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type: Range
persistent identity: range2470058
display id: range2470058
version: 1
direction: direction/forward
top level: BBa_K1689012
start: 37
end: 54
orientation: inline

GGGGS*2 Linker
type: SequenceAnnotation
persistent identity: annotation2470090
display id: annotation2470090
version: 1
top level: BBa_K1689012
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1689012/annotation2470090/range2470090/1

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type: Range
persistent identity: range2470090
display id: range2470090
version: 1
direction: direction/forward
top level: BBa_K1689012
start: 4156
end: 4185
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2477771
display id: annotation2477771
version: 1
top level: BBa_K1689012
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1689012/annotation2477771/range2477771/1

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type: Range
persistent identity: range2477771
display id: range2477771
version: 1
direction: direction/forward
top level: BBa_K1689012
start: 4699
end: 4701
orientation: inline

Nlact for N terminal of β-Lactamase
type: SequenceAnnotation
persistent identity: annotation2470104
display id: annotation2470104
version: 1
top level: BBa_K1689012
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1689012/annotation2470104/range2470104/1

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type: Range
persistent identity: range2470104
display id: range2470104
version: 1
direction: direction/forward
top level: BBa_K1689012
start: 4186
end: 4698
orientation: inline

dCas9-Nlact
type: SequenceAnnotation
persistent identity: annotation2470073
display id: annotation2470073
version: 1
top level: BBa_K1689012
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1689012/annotation2470073/range2470073/1

https://synbiohub.org/public/igem/BBa_K1689012/annotation2470073/range2470073/1
type: Range
persistent identity: range2470073
display id: range2470073
version: 1
direction: direction/forward
top level: BBa_K1689012
start: 34
end: 4701
orientation: inline

random spacer
type: SequenceAnnotation
persistent identity: annotation2477770
display id: annotation2477770
version: 1
top level: BBa_K1689012
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1689012/annotation2477770/range2477770/1

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type: Range
persistent identity: range2477770
display id: range2477770
version: 1
direction: direction/forward
top level: BBa_K1689012
start: 1
end: 33
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2477769
display id: annotation2477769
version: 1
top level: BBa_K1689012
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1689012/annotation2477769/range2477769/1

https://synbiohub.org/public/igem/BBa_K1689012/annotation2477769/range2477769/1
type: Range
persistent identity: range2477769
display id: range2477769
version: 1
direction: direction/forward
top level: BBa_K1689012
start: 34
end: 36
orientation: inline

random spacer
type: SequenceAnnotation
persistent identity: annotation2477772
display id: annotation2477772
version: 1
top level: BBa_K1689012
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1689012/annotation2477772/range2477772/1

https://synbiohub.org/public/igem/BBa_K1689012/annotation2477772/range2477772/1
type: Range
persistent identity: range2477772
display id: range2477772
version: 1
direction: direction/forward
top level: BBa_K1689012
start: 4702
end: 4772
orientation: inline

BBa_K1362054
type: ComponentDefinition
persistent identity: BBa K1362054
display id: BBa_K1362054
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SsrA degradation tag as RFC[105] C-terminal insert
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B??scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch??fer, Carolin Schmelas, Silvan Schmitz, Max Waldha
created: 2014-10-07T11:00:00Z
modified: 2015-05-08T01:10:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1738_
m user id: 0
owner id: 22920
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1362425
component: BBa_K1362421
component: BBa_M0050
component: BBa_K1362419
component: BBa_K1362423
component: BBa_K1362433
component: BBa_K1362426
component: BBa_K1362438
sequence annotation: BBa_K1362425
sequence annotation: BBa_K1362421
sequence annotation: BBa_K1362419
sequence annotation: BBa_K1362433
sequence annotation: BBa_K1362423
sequence annotation: BBa_K1362426
sequence annotation: BBa_M0050
sequence annotation: BBa_K1362438
sequence: https://synbiohub.org/public/igem/BBa_K1362054_sequence/1

BBa_K1362425
type: Component
persistent identity: component2409693
display id: component2409693
version: 1
top level: BBa_K1362054
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362425/1

BBa_K1362421
type: Component
persistent identity: component2409701
display id: component2409701
version: 1
top level: BBa_K1362054
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362421/1

BBa_M0050
type: Component
persistent identity: component2409699
display id: component2409699
version: 1
top level: BBa_K1362054
access: public
definition: https://synbiohub.org/public/igem/BBa_M0050/1

BBa_K1362419
type: Component
persistent identity: component2409694
display id: component2409694
version: 1
top level: BBa_K1362054
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362419/1

BBa_K1362423
type: Component
persistent identity: component2409702
display id: component2409702
version: 1
top level: BBa_K1362054
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362423/1

BBa_K1362433
type: Component
persistent identity: component2409696
display id: component2409696
version: 1
top level: BBa_K1362054
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362433/1

BBa_K1362426
type: Component
persistent identity: component2409703
display id: component2409703
version: 1
top level: BBa_K1362054
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362426/1

BBa_K1362438
type: Component
persistent identity: component2409697
display id: component2409697
version: 1
top level: BBa_K1362054
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362438/1

BBa_K1362425
type: SequenceAnnotation
persistent identity: annotation2409693
display id: annotation2409693
version: 1
top level: BBa_K1362054
location: https://synbiohub.org/public/igem/BBa_K1362054/annotation2409693/range2409693/1
component: BBa_K1362425

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type: Range
persistent identity: range2409693
display id: range2409693
version: 1
direction: direction/reverse
top level: BBa_K1362054
start: 1
end: 6
orientation: inline

BBa_K1362421
type: SequenceAnnotation
persistent identity: annotation2409701
display id: annotation2409701
version: 1
top level: BBa_K1362054
location: https://synbiohub.org/public/igem/BBa_K1362054/annotation2409701/range2409701/1
component: BBa_K1362421

https://synbiohub.org/public/igem/BBa_K1362054/annotation2409701/range2409701/1
type: Range
persistent identity: range2409701
display id: range2409701
version: 1
direction: direction/reverse
top level: BBa_K1362054
start: 62
end: 65
orientation: inline

BBa_K1362419
type: SequenceAnnotation
persistent identity: annotation2409694
display id: annotation2409694
version: 1
top level: BBa_K1362054
location: https://synbiohub.org/public/igem/BBa_K1362054/annotation2409694/range2409694/1
component: BBa_K1362419

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type: Range
persistent identity: range2409694
display id: range2409694
version: 1
direction: direction/reverse
top level: BBa_K1362054
start: 7
end: 10
orientation: inline

BBa_K1362433
type: SequenceAnnotation
persistent identity: annotation2409696
display id: annotation2409696
version: 1
top level: BBa_K1362054
location: https://synbiohub.org/public/igem/BBa_K1362054/annotation2409696/range2409696/1
component: BBa_K1362433

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type: Range
persistent identity: range2409696
display id: range2409696
version: 1
direction: direction/reverse
top level: BBa_K1362054
start: 11
end: 19
orientation: inline

BBa_K1362423
type: SequenceAnnotation
persistent identity: annotation2409702
display id: annotation2409702
version: 1
top level: BBa_K1362054
location: https://synbiohub.org/public/igem/BBa_K1362054/annotation2409702/range2409702/1
component: BBa_K1362423

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type: Range
persistent identity: range2409702
display id: range2409702
version: 1
direction: direction/reverse
top level: BBa_K1362054
start: 66
end: 72
orientation: inline

BBa_K1362426
type: SequenceAnnotation
persistent identity: annotation2409703
display id: annotation2409703
version: 1
top level: BBa_K1362054
location: https://synbiohub.org/public/igem/BBa_K1362054/annotation2409703/range2409703/1
component: BBa_K1362426

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type: Range
persistent identity: range2409703
display id: range2409703
version: 1
direction: direction/reverse
top level: BBa_K1362054
start: 73
end: 76
orientation: inline

BBa_M0050
type: SequenceAnnotation
persistent identity: annotation2409699
display id: annotation2409699
version: 1
top level: BBa_K1362054
location: https://synbiohub.org/public/igem/BBa_K1362054/annotation2409699/range2409699/1
component: BBa_M0050

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type: Range
persistent identity: range2409699
display id: range2409699
version: 1
direction: direction/reverse
top level: BBa_K1362054
start: 29
end: 61
orientation: inline

BBa_K1362438
type: SequenceAnnotation
persistent identity: annotation2409697
display id: annotation2409697
version: 1
top level: BBa_K1362054
location: https://synbiohub.org/public/igem/BBa_K1362054/annotation2409697/range2409697/1
component: BBa_K1362438

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type: Range
persistent identity: range2409697
display id: range2409697
version: 1
direction: direction/reverse
top level: BBa_K1362054
start: 20
end: 28
orientation: inline

BBa_K1325001
type: ComponentDefinition
persistent identity: BBa K1325001
display id: BBa_K1325001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ranscriptionfactor
creator: Ke Xu
created: 2014-09-26T11:00:00Z
modified: 2015-05-08T01:09:54Z
discontinued: false
dominant: false
group u list: _1700_
m user id: 0
owner id: 23253
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: ranscriptionfactor，the ipgC???mxiE complex activates transcriptionfrom the pipaH* promoter
mutable notes: no
mutable provenance: gene clusters encoding type III secretion systems
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1325001_sequence/1

BFRM52H
type: ComponentDefinition
persistent identity: BBa K1438001
display id: BBa_K1438001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Bacterioferritin (BFR) M52H heme-deletion
creator: Johann Bauerfeind
created: 2014-10-05T11:00:00Z
modified: 2015-07-22T02:13:13Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1816_
m user id: 4206
owner id: 19714
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: ATGAAAGGTGATACTAAAGTTATAAATTATCTCAACAAACTGTTGGGAAATGAGCTTGTCGCAATCAATCAGTACTTTCTTCATGCCCGGATGTTTAAAAACTGGGGTCTCAAACGTCTCAATGATGTGGAGTATCATGAATCCATTGATGAGCACAAACACGCCGATCGTTATATTGAGCGCATTCTTTTTCTGGAAGGTCTTCCAAACTTACAGGACCTGGGCAAACTGAACATTGGTGAAGATGTTGAGGAAATGCTGCGTTCTGATCTGGCACTTGAGCTGGATGGCGCGAAGAATTTGCGTGAGGCAATTGGTTATGCCGATAGCGTTCATGATTACGTCAGCCGCGATATGATGATAGAAATTTTGCGCGATGAAGAAGGCCATATCGACTGGCTGGAAACGGAACTTGATCTGATTCAGAAGATGGGACTGCAAAATTATCTGCAAGCACAGATTCGCGAAGAAGGTTAA
mutable notes: The M52H Mutagensis leads to an deletion of the BFR heme residue. As a result, BFR will have a reduced iron release rate and keep it's iron content inside of the protein scaffold.
mutable provenance: Escherichia coli Nissle 1917
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1438001_sequence/1

BBa_K1362205
type: ComponentDefinition
persistent identity: BBa K1362205
display id: BBa_K1362205
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Sortase A non-circularization construct (with Smt3 and His6; without RBS)
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Büscher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schäfer, Carolin Schmelas, Silvan Schmitz, Max Waldha
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:05Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1738_
m user id: 0
owner id: 22951
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: TODO
mutable notes: TODO
mutable provenance: TODO
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1362429
component: BBa_K1362456
component: BBa_K1362453
component: BBa_J70594
component: BBa_K1362424
component: BBa_K1362449
component: BBa_J04450
component: BBa_K1362446
component: BBa_K1362448
component: BBa_K1362459
component: BBa_K1362458
component: BBa_K1362447
sequence annotation: BBa_K1362446
sequence annotation: BBa_K1362456
sequence annotation: BBa_K1362448
sequence annotation: BBa_K1362459
sequence annotation: BBa_K1362447
sequence annotation: BBa_K1362449
sequence annotation: BBa_K1362458
sequence annotation: BBa_K1362453
sequence annotation: BBa_K1362424
sequence annotation: BBa_J70594
sequence annotation: BBa_J04450
sequence annotation: BBa_K1362429
sequence: https://synbiohub.org/public/igem/BBa_K1362205_sequence/1

BBa_K1362429
type: Component
persistent identity: component2410853
display id: component2410853
version: 1
top level: BBa_K1362205
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362429/1

BBa_K1362456
type: Component
persistent identity: component2410850
display id: component2410850
version: 1
top level: BBa_K1362205
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362456/1

BBa_K1362453
type: Component
persistent identity: component2410854
display id: component2410854
version: 1
top level: BBa_K1362205
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362453/1

BBa_J70594
type: Component
persistent identity: component2410877
display id: component2410877
version: 1
top level: BBa_K1362205
access: public
definition: https://synbiohub.org/public/igem/BBa_J70594/1

BBa_K1362424
type: Component
persistent identity: component2410874
display id: component2410874
version: 1
top level: BBa_K1362205
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362424/1

BBa_K1362449
type: Component
persistent identity: component2410849
display id: component2410849
version: 1
top level: BBa_K1362205
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362449/1

BBa_J04450
type: Component
persistent identity: component2410873
display id: component2410873
version: 1
top level: BBa_K1362205
access: public
definition: BBa_J04450

BBa_K1362446
type: Component
persistent identity: component2410847
display id: component2410847
version: 1
top level: BBa_K1362205
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362446/1

BBa_K1362448
type: Component
persistent identity: component2410851
display id: component2410851
version: 1
top level: BBa_K1362205
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362448/1

BBa_K1362459
type: Component
persistent identity: component2410875
display id: component2410875
version: 1
top level: BBa_K1362205
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362459/1

BBa_K1362458
type: Component
persistent identity: component2410848
display id: component2410848
version: 1
top level: BBa_K1362205
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362458/1

BBa_K1362447
type: Component
persistent identity: component2410855
display id: component2410855
version: 1
top level: BBa_K1362205
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362447/1

BBa_K1362446
type: SequenceAnnotation
persistent identity: annotation2410847
display id: annotation2410847
version: 1
top level: BBa_K1362205
location: https://synbiohub.org/public/igem/BBa_K1362205/annotation2410847/range2410847/1
component: BBa_K1362446

https://synbiohub.org/public/igem/BBa_K1362205/annotation2410847/range2410847/1
type: Range
persistent identity: range2410847
display id: range2410847
version: 1
direction: direction/reverse
top level: BBa_K1362205
start: 1
end: 12
orientation: inline

BBa_K1362456
type: SequenceAnnotation
persistent identity: annotation2410850
display id: annotation2410850
version: 1
top level: BBa_K1362205
location: https://synbiohub.org/public/igem/BBa_K1362205/annotation2410850/range2410850/1
component: BBa_K1362456

https://synbiohub.org/public/igem/BBa_K1362205/annotation2410850/range2410850/1
type: Range
persistent identity: range2410850
display id: range2410850
version: 1
direction: direction/reverse
top level: BBa_K1362205
start: 40
end: 57
orientation: inline

BBa_K1362448
type: SequenceAnnotation
persistent identity: annotation2410851
display id: annotation2410851
version: 1
top level: BBa_K1362205
location: https://synbiohub.org/public/igem/BBa_K1362205/annotation2410851/range2410851/1
component: BBa_K1362448

https://synbiohub.org/public/igem/BBa_K1362205/annotation2410851/range2410851/1
type: Range
persistent identity: range2410851
display id: range2410851
version: 1
direction: direction/reverse
top level: BBa_K1362205
start: 58
end: 72
orientation: inline

BBa_K1362459
type: SequenceAnnotation
persistent identity: annotation2410875
display id: annotation2410875
version: 1
top level: BBa_K1362205
location: https://synbiohub.org/public/igem/BBa_K1362205/annotation2410875/range2410875/1
component: BBa_K1362459

https://synbiohub.org/public/igem/BBa_K1362205/annotation2410875/range2410875/1
type: Range
persistent identity: range2410875
display id: range2410875
version: 1
direction: direction/reverse
top level: BBa_K1362205
start: 1470
end: 1481
orientation: inline

BBa_K1362447
type: SequenceAnnotation
persistent identity: annotation2410855
display id: annotation2410855
version: 1
top level: BBa_K1362205
location: https://synbiohub.org/public/igem/BBa_K1362205/annotation2410855/range2410855/1
component: BBa_K1362447

https://synbiohub.org/public/igem/BBa_K1362205/annotation2410855/range2410855/1
type: Range
persistent identity: range2410855
display id: range2410855
version: 1
direction: direction/reverse
top level: BBa_K1362205
start: 388
end: 393
orientation: inline

BBa_K1362449
type: SequenceAnnotation
persistent identity: annotation2410849
display id: annotation2410849
version: 1
top level: BBa_K1362205
location: https://synbiohub.org/public/igem/BBa_K1362205/annotation2410849/range2410849/1
component: BBa_K1362449

https://synbiohub.org/public/igem/BBa_K1362205/annotation2410849/range2410849/1
type: Range
persistent identity: range2410849
display id: range2410849
version: 1
direction: direction/reverse
top level: BBa_K1362205
start: 31
end: 39
orientation: inline

BBa_K1362458
type: SequenceAnnotation
persistent identity: annotation2410848
display id: annotation2410848
version: 1
top level: BBa_K1362205
location: https://synbiohub.org/public/igem/BBa_K1362205/annotation2410848/range2410848/1
component: BBa_K1362458

https://synbiohub.org/public/igem/BBa_K1362205/annotation2410848/range2410848/1
type: Range
persistent identity: range2410848
display id: range2410848
version: 1
direction: direction/reverse
top level: BBa_K1362205
start: 13
end: 30
orientation: inline

BBa_K1362453
type: SequenceAnnotation
persistent identity: annotation2410854
display id: annotation2410854
version: 1
top level: BBa_K1362205
location: https://synbiohub.org/public/igem/BBa_K1362205/annotation2410854/range2410854/1
component: BBa_K1362453

https://synbiohub.org/public/igem/BBa_K1362205/annotation2410854/range2410854/1
type: Range
persistent identity: range2410854
display id: range2410854
version: 1
direction: direction/reverse
top level: BBa_K1362205
start: 367
end: 387
orientation: inline

BBa_K1362424
type: SequenceAnnotation
persistent identity: annotation2410874
display id: annotation2410874
version: 1
top level: BBa_K1362205
location: https://synbiohub.org/public/igem/BBa_K1362205/annotation2410874/range2410874/1
component: BBa_K1362424

https://synbiohub.org/public/igem/BBa_K1362205/annotation2410874/range2410874/1
type: Range
persistent identity: range2410874
display id: range2410874
version: 1
direction: direction/reverse
top level: BBa_K1362205
start: 1463
end: 1469
orientation: inline

BBa_J70594
type: SequenceAnnotation
persistent identity: annotation2410877
display id: annotation2410877
version: 1
top level: BBa_K1362205
location: https://synbiohub.org/public/igem/BBa_K1362205/annotation2410877/range2410877/1
component: BBa_J70594

https://synbiohub.org/public/igem/BBa_K1362205/annotation2410877/range2410877/1
type: Range
persistent identity: range2410877
display id: range2410877
version: 1
direction: direction/reverse
top level: BBa_K1362205
start: 1482
end: 1487
orientation: inline

BBa_J04450
type: SequenceAnnotation
persistent identity: annotation2410873
display id: annotation2410873
version: 1
top level: BBa_K1362205
location: https://synbiohub.org/public/igem/BBa_K1362205/annotation2410873/range2410873/1
component: BBa_J04450

https://synbiohub.org/public/igem/BBa_K1362205/annotation2410873/range2410873/1
type: Range
persistent identity: range2410873
display id: range2410873
version: 1
direction: direction/reverse
top level: BBa_K1362205
start: 394
end: 1462
orientation: inline

BBa_K1362429
type: SequenceAnnotation
persistent identity: annotation2410853
display id: annotation2410853
version: 1
top level: BBa_K1362205
location: https://synbiohub.org/public/igem/BBa_K1362205/annotation2410853/range2410853/1
component: BBa_K1362429

https://synbiohub.org/public/igem/BBa_K1362205/annotation2410853/range2410853/1
type: Range
persistent identity: range2410853
display id: range2410853
version: 1
direction: direction/reverse
top level: BBa_K1362205
start: 73
end: 366
orientation: inline

aes-SH3lig
type: ComponentDefinition
persistent identity: BBa K1602034
display id: BBa_K1602034
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: aes-SH3lig
creator: Jasmin Weber, Michelle Zoeller, Marcus Geissler, Stefan Bohn, Simon Linder, Sebastian Barthel
created: 2015-09-15T11:00:00Z
modified: 2015-09-16T07:54:44Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2019_
m user id: 22022
owner id: 22022
owning group id: 9
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1602034_sequence/1

BBa_K1692032
type: ComponentDefinition
persistent identity: BBa K1692032
display id: BBa_K1692032
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: amilCP blue chromoprotein with RBS and promoter
creator: Jack Ryan Takahashi
created: 2015-09-13T11:00:00Z
modified: 2015-09-18T05:00:27Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2110_
m user id: 26427
owner id: 26427
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This plasmid contains the amilCP blue chromoprotein gene found in part BBa_K592009 and the RBS and promoter from part BBa_K608002.
mutable notes: N/A
mutable provenance: This part is made from parts BBa_K608002 and BBa_K592009.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_K592009
component: BBa_K608002
sequence annotation: BBa_K608002
sequence annotation: BBa_K592009
sequence: https://synbiohub.org/public/igem/BBa_K1692032_sequence/1

BBa_K592009
type: Component
persistent identity: component2455198
display id: component2455198
version: 1
top level: BBa_K1692032
access: public
definition: https://synbiohub.org/public/igem/BBa_K592009/1

BBa_K608002
type: Component
persistent identity: component2455196
display id: component2455196
version: 1
top level: BBa_K1692032
access: public
definition: https://synbiohub.org/public/igem/BBa_K608002/1

BBa_K608002
type: SequenceAnnotation
persistent identity: annotation2455196
display id: annotation2455196
version: 1
top level: BBa_K1692032
location: https://synbiohub.org/public/igem/BBa_K1692032/annotation2455196/range2455196/1
component: BBa_K608002

https://synbiohub.org/public/igem/BBa_K1692032/annotation2455196/range2455196/1
type: Range
persistent identity: range2455196
display id: range2455196
version: 1
direction: direction/reverse
top level: BBa_K1692032
start: 1
end: 55
orientation: inline

BBa_K592009
type: SequenceAnnotation
persistent identity: annotation2455198
display id: annotation2455198
version: 1
top level: BBa_K1692032
location: https://synbiohub.org/public/igem/BBa_K1692032/annotation2455198/range2455198/1
component: BBa_K592009

https://synbiohub.org/public/igem/BBa_K1692032/annotation2455198/range2455198/1
type: Range
persistent identity: range2455198
display id: range2455198
version: 1
direction: direction/reverse
top level: BBa_K1692032
start: 62
end: 730
orientation: inline

CircSortA*
type: ComponentDefinition
persistent identity: BBa K1362003
display id: BBa_K1362003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Sortase A circularization construct (with Smt3 and His6)
creator: Silvan Schmitz
created: 2014-10-08T11:00:00Z
modified: 2015-05-08T01:10:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1738_
m user id: 0
owner id: 22951
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: TODO
mutable notes: TODO
mutable provenance: TODO
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1362203
component: BBa_K1362090
sequence annotation: BBa_K1362203
sequence annotation: BBa_K1362090
sequence: https://synbiohub.org/public/igem/BBa_K1362003_sequence/1

BBa_K1362203
type: Component
persistent identity: component2409011
display id: component2409011
version: 1
top level: CircSortA*
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362203/1

BBa_K1362090
type: Component
persistent identity: component2408981
display id: component2408981
version: 1
top level: CircSortA*
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362090/1

BBa_K1362203
type: SequenceAnnotation
persistent identity: annotation2409011
display id: annotation2409011
version: 1
top level: CircSortA*
location: https://synbiohub.org/public/igem/BBa_K1362003/annotation2409011/range2409011/1
component: BBa_K1362203

https://synbiohub.org/public/igem/BBa_K1362003/annotation2409011/range2409011/1
type: Range
persistent identity: range2409011
display id: range2409011
version: 1
direction: direction/reverse
top level: CircSortA*
start: 36
end: 1477
orientation: inline

BBa_K1362090
type: SequenceAnnotation
persistent identity: annotation2408981
display id: annotation2408981
version: 1
top level: CircSortA*
location: https://synbiohub.org/public/igem/BBa_K1362003/annotation2408981/range2408981/1
component: BBa_K1362090

https://synbiohub.org/public/igem/BBa_K1362003/annotation2408981/range2408981/1
type: Range
persistent identity: range2408981
display id: range2408981
version: 1
direction: direction/reverse
top level: CircSortA*
start: 1
end: 29
orientation: inline

BBa_K1789009
type: ComponentDefinition
persistent identity: BBa K1789009
display id: BBa_K1789009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TALE2
creator: Xinyuan Qiu
created: 2015-09-13T11:00:00Z
modified: 2015-09-14T07:20:22Z
discontinued: false
dominant: false
group u list: _2214_
m user id: 21134
owner id: 21134
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This is a engineered TAL effector that can bind with the DNA sequence 5'-GATAAACACCTTTC-3'
mutable notes: The part sequence should be able to correctly and efficiently bind with corresponding DNA, so we used bioinformatics tools to help us designing this sequence.
mutable provenance: This part is built and standardized by ourselves using GATEWAY technique.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: TALE2
sequence annotation: DNA Binding Domain
sequence: https://synbiohub.org/public/igem/BBa_K1789009_sequence/1

TALE2
type: SequenceAnnotation
persistent identity: annotation2454121
display id: annotation2454121
version: 1
top level: BBa_K1789009
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1789009/annotation2454121/range2454121/1

https://synbiohub.org/public/igem/BBa_K1789009/annotation2454121/range2454121/1
type: Range
persistent identity: range2454121
display id: range2454121
version: 1
direction: direction/forward
top level: BBa_K1789009
start: 1
end: 2088
orientation: inline

DNA Binding Domain
type: SequenceAnnotation
persistent identity: annotation2454120
display id: annotation2454120
version: 1
top level: BBa_K1789009
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1789009/annotation2454120/range2454120/1

https://synbiohub.org/public/igem/BBa_K1789009/annotation2454120/range2454120/1
type: Range
persistent identity: range2454120
display id: range2454120
version: 1
direction: direction/forward
top level: BBa_K1789009
start: 514
end: 1872
orientation: inline

BBa_K1614002
type: ComponentDefinition
persistent identity: BBa K1614002
display id: BBa_K1614002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: transcription cassette of BBF RFC 110
creator: Stefan Holderbach
created: 2015-09-13T11:00:00Z
modified: 2015-09-16T05:48:58Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2031_
m user id: 24664
owner id: 12380
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Unit for RFC 110 assembly, clonable in RFC 10 vectors like pSB1C3.
mutable notes: T7 promoter for expression
mutable provenance: Standard assembly construct for RFC 110 assembly
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence: https://synbiohub.org/public/igem/BBa_K1614002_sequence/1

C2HP/RGE
type: ComponentDefinition
persistent identity: BBa K1442107
display id: BBa_K1442107
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: C2HP Testing Module (reverse GFP)
creator: Dan Goss
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:27Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1820_
m user id: 0
owner id: 23055
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: ...
mutable notes: ...
mutable provenance: ...
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1442107_sequence/1

BBa_K1497024
type: ComponentDefinition
persistent identity: BBa K1497024
display id: BBa_K1497024
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GBD-Domain
creator: Sascha Hein, Rene Sahm
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:45Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1877_
m user id: 0
owner id: 12466
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: d
mutable notes: d
mutable provenance: d
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1497024_sequence/1

SspDnaB(N)
type: ComponentDefinition
persistent identity: BBa K1362140
display id: BBa_K1362140
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS + SspDnaB N-intein RFC[105] assembly construct (with His6)
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B??scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch??fer, Carolin Schmelas, Silvan Schmitz, Max Waldha
created: 2014-10-08T11:00:00Z
modified: 2015-05-08T01:10:05Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1738_
m user id: 0
owner id: 12377
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: ???
mutable notes: ???
mutable provenance: ???
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1362422
component: BBa_K1362417
component: BBa_K1362437
component: BBa_K1362415
component: BBa_K1362408
component: BBa_G0000
component: BBa_K1362430
component: BBa_K1362090
component: BBa_K1362414
component: BBa_K1362421
sequence annotation: BBa_G0000
sequence annotation: BBa_K1362090
sequence annotation: BBa_K1362422
sequence annotation: BBa_K1362415
sequence annotation: BBa_K1362414
sequence annotation: BBa_K1362430
sequence annotation: BBa_K1362408
sequence annotation: BBa_K1362437
sequence annotation: BBa_K1362417
sequence annotation: BBa_K1362421
sequence: https://synbiohub.org/public/igem/BBa_K1362140_sequence/1

BBa_K1362422
type: Component
persistent identity: component2410568
display id: component2410568
version: 1
top level: SspDnaB(N)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362422/1

BBa_K1362417
type: Component
persistent identity: component2410573
display id: component2410573
version: 1
top level: SspDnaB(N)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362417/1

BBa_K1362437
type: Component
persistent identity: component2410580
display id: component2410580
version: 1
top level: SspDnaB(N)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362437/1

BBa_K1362415
type: Component
persistent identity: component2410569
display id: component2410569
version: 1
top level: SspDnaB(N)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362415/1

BBa_K1362408
type: Component
persistent identity: component2410570
display id: component2410570
version: 1
top level: SspDnaB(N)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362408/1

BBa_G0000
type: Component
persistent identity: component2410546
display id: component2410546
version: 1
top level: SspDnaB(N)
access: public
definition: https://synbiohub.org/public/igem/BBa_G0000/1

BBa_K1362430
type: Component
persistent identity: component2410576
display id: component2410576
version: 1
top level: SspDnaB(N)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362430/1

BBa_K1362090
type: Component
persistent identity: component2410545
display id: component2410545
version: 1
top level: SspDnaB(N)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362090/1

BBa_K1362414
type: Component
persistent identity: component2410547
display id: component2410547
version: 1
top level: SspDnaB(N)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362414/1

BBa_K1362421
type: Component
persistent identity: component2410578
display id: component2410578
version: 1
top level: SspDnaB(N)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362421/1

BBa_G0000
type: SequenceAnnotation
persistent identity: annotation2410546
display id: annotation2410546
version: 1
top level: SspDnaB(N)
location: https://synbiohub.org/public/igem/BBa_K1362140/annotation2410546/range2410546/1
component: BBa_G0000

https://synbiohub.org/public/igem/BBa_K1362140/annotation2410546/range2410546/1
type: Range
persistent identity: range2410546
display id: range2410546
version: 1
direction: direction/reverse
top level: SspDnaB(N)
start: 30
end: 35
orientation: inline

BBa_K1362090
type: SequenceAnnotation
persistent identity: annotation2410545
display id: annotation2410545
version: 1
top level: SspDnaB(N)
location: https://synbiohub.org/public/igem/BBa_K1362140/annotation2410545/range2410545/1
component: BBa_K1362090

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type: Range
persistent identity: range2410545
display id: range2410545
version: 1
direction: direction/reverse
top level: SspDnaB(N)
start: 1
end: 29
orientation: inline

BBa_K1362422
type: SequenceAnnotation
persistent identity: annotation2410568
display id: annotation2410568
version: 1
top level: SspDnaB(N)
location: https://synbiohub.org/public/igem/BBa_K1362140/annotation2410568/range2410568/1
component: BBa_K1362422

https://synbiohub.org/public/igem/BBa_K1362140/annotation2410568/range2410568/1
type: Range
persistent identity: range2410568
display id: range2410568
version: 1
direction: direction/reverse
top level: SspDnaB(N)
start: 39
end: 1121
orientation: inline

BBa_K1362415
type: SequenceAnnotation
persistent identity: annotation2410569
display id: annotation2410569
version: 1
top level: SspDnaB(N)
location: https://synbiohub.org/public/igem/BBa_K1362140/annotation2410569/range2410569/1
component: BBa_K1362415

https://synbiohub.org/public/igem/BBa_K1362140/annotation2410569/range2410569/1
type: Range
persistent identity: range2410569
display id: range2410569
version: 1
direction: direction/reverse
top level: SspDnaB(N)
start: 1122
end: 1125
orientation: inline

BBa_K1362414
type: SequenceAnnotation
persistent identity: annotation2410547
display id: annotation2410547
version: 1
top level: SspDnaB(N)
location: https://synbiohub.org/public/igem/BBa_K1362140/annotation2410547/range2410547/1
component: BBa_K1362414

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type: Range
persistent identity: range2410547
display id: range2410547
version: 1
direction: direction/reverse
top level: SspDnaB(N)
start: 36
end: 38
orientation: inline

BBa_K1362430
type: SequenceAnnotation
persistent identity: annotation2410576
display id: annotation2410576
version: 1
top level: SspDnaB(N)
location: https://synbiohub.org/public/igem/BBa_K1362140/annotation2410576/range2410576/1
component: BBa_K1362430

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type: Range
persistent identity: range2410576
display id: range2410576
version: 1
direction: direction/reverse
top level: SspDnaB(N)
start: 1167
end: 1190
orientation: inline

BBa_K1362408
type: SequenceAnnotation
persistent identity: annotation2410570
display id: annotation2410570
version: 1
top level: SspDnaB(N)
location: https://synbiohub.org/public/igem/BBa_K1362140/annotation2410570/range2410570/1
component: BBa_K1362408

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type: Range
persistent identity: range2410570
display id: range2410570
version: 1
direction: direction/reverse
top level: SspDnaB(N)
start: 1126
end: 1160
orientation: inline

BBa_K1362437
type: SequenceAnnotation
persistent identity: annotation2410580
display id: annotation2410580
version: 1
top level: SspDnaB(N)
location: https://synbiohub.org/public/igem/BBa_K1362140/annotation2410580/range2410580/1
component: BBa_K1362437

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type: Range
persistent identity: range2410580
display id: range2410580
version: 1
direction: direction/reverse
top level: SspDnaB(N)
start: 1195
end: 1197
orientation: inline

BBa_K1362417
type: SequenceAnnotation
persistent identity: annotation2410573
display id: annotation2410573
version: 1
top level: SspDnaB(N)
location: https://synbiohub.org/public/igem/BBa_K1362140/annotation2410573/range2410573/1
component: BBa_K1362417

https://synbiohub.org/public/igem/BBa_K1362140/annotation2410573/range2410573/1
type: Range
persistent identity: range2410573
display id: range2410573
version: 1
direction: direction/reverse
top level: SspDnaB(N)
start: 1161
end: 1166
orientation: inline

BBa_K1362421
type: SequenceAnnotation
persistent identity: annotation2410578
display id: annotation2410578
version: 1
top level: SspDnaB(N)
location: https://synbiohub.org/public/igem/BBa_K1362140/annotation2410578/range2410578/1
component: BBa_K1362421

https://synbiohub.org/public/igem/BBa_K1362140/annotation2410578/range2410578/1
type: Range
persistent identity: range2410578
display id: range2410578
version: 1
direction: direction/reverse
top level: SspDnaB(N)
start: 1191
end: 1194
orientation: inline

BBa_K1321338
type: ComponentDefinition
persistent identity: BBa K1321338
display id: BBa_K1321338
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 Expression vector
creator: Chris N Micklem
created: 2014-10-07T11:00:00Z
modified: 2015-05-08T01:09:53Z
discontinued: false
dominant: false
group u list: _1696_
m user id: 0
owner id: 20830
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: asdfasdf
mutable notes: asdf
mutable provenance: asdf
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_I719005
component: BBa_B0034
sequence annotation: BBa_I719005
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K1321338_sequence/1

BBa_I719005
type: Component
persistent identity: component2402299
display id: component2402299
version: 1
top level: BBa_K1321338
access: public
definition: https://synbiohub.org/public/igem/BBa_I719005/1

BBa_B0034
type: Component
persistent identity: component2402301
display id: component2402301
version: 1
top level: BBa_K1321338
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_I719005
type: SequenceAnnotation
persistent identity: annotation2402299
display id: annotation2402299
version: 1
top level: BBa_K1321338
location: https://synbiohub.org/public/igem/BBa_K1321338/annotation2402299/range2402299/1
component: BBa_I719005

https://synbiohub.org/public/igem/BBa_K1321338/annotation2402299/range2402299/1
type: Range
persistent identity: range2402299
display id: range2402299
version: 1
direction: direction/reverse
top level: BBa_K1321338
start: 1
end: 23
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2402301
display id: annotation2402301
version: 1
top level: BBa_K1321338
location: https://synbiohub.org/public/igem/BBa_K1321338/annotation2402301/range2402301/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1321338/annotation2402301/range2402301/1
type: Range
persistent identity: range2402301
display id: range2402301
version: 1
direction: direction/reverse
top level: BBa_K1321338
start: 32
end: 43
orientation: inline

BBa_K1674003
type: ComponentDefinition
persistent identity: BBa K1674003
display id: BBa_K1674003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: tsPurple Reporter + 3-alpha-HSD (RBS + Coding + Terminator)
creator: Adi Yannai
created: 2015-09-14T11:00:00Z
modified: 2015-09-18T02:25:56Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2092_
m user id: 25765
owner id: 25765
owning group id: 9
status: status/Planning
bookmark: false
mutable description: tsPurple Reporter + 3α- HSD
mutable notes: tsPurple Reporter + 3α- HSD
mutable provenance: tsPurple Reporter + 3α- HSD
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1357008
component: BBa_K1321338
component: BBa_K1674001
sequence annotation: BBa_K1321338
sequence annotation: BBa_K1674001
sequence annotation: BBa_K1357008
sequence: https://synbiohub.org/public/igem/BBa_K1674003_sequence/1

BBa_K1357008
type: Component
persistent identity: component2457671
display id: component2457671
version: 1
top level: BBa_K1674003
access: public
definition: https://synbiohub.org/public/igem/BBa_K1357008/1

BBa_K1321338
type: Component
persistent identity: component2457658
display id: component2457658
version: 1
top level: BBa_K1674003
access: public
definition: BBa_K1321338

BBa_K1674001
type: Component
persistent identity: component2457659
display id: component2457659
version: 1
top level: BBa_K1674003
access: public
definition: 3alpha-HSD

BBa_K1321338
type: SequenceAnnotation
persistent identity: annotation2457658
display id: annotation2457658
version: 1
top level: BBa_K1674003
location: https://synbiohub.org/public/igem/BBa_K1674003/annotation2457658/range2457658/1
component: BBa_K1321338

https://synbiohub.org/public/igem/BBa_K1674003/annotation2457658/range2457658/1
type: Range
persistent identity: range2457658
display id: range2457658
version: 1
direction: direction/reverse
top level: BBa_K1674003
start: 1
end: 43
orientation: inline

BBa_K1674001
type: SequenceAnnotation
persistent identity: annotation2457659
display id: annotation2457659
version: 1
top level: BBa_K1674003
location: https://synbiohub.org/public/igem/BBa_K1674003/annotation2457659/range2457659/1
component: BBa_K1674001

https://synbiohub.org/public/igem/BBa_K1674003/annotation2457659/range2457659/1
type: Range
persistent identity: range2457659
display id: range2457659
version: 1
direction: direction/reverse
top level: BBa_K1674003
start: 50
end: 1018
orientation: inline

BBa_K1357008
type: SequenceAnnotation
persistent identity: annotation2457671
display id: annotation2457671
version: 1
top level: BBa_K1674003
location: https://synbiohub.org/public/igem/BBa_K1674003/annotation2457671/range2457671/1
component: BBa_K1357008

https://synbiohub.org/public/igem/BBa_K1674003/annotation2457671/range2457671/1
type: Range
persistent identity: range2457671
display id: range2457671
version: 1
direction: direction/reverse
top level: BBa_K1674003
start: 1027
end: 1871
orientation: inline

BBa_K1497011
type: ComponentDefinition
persistent identity: BBa K1497011
display id: BBa_K1497011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0034-ANS
creator: Sascha Hein, Sven Jager, Thomas Dohmen
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1877_
m user id: 0
owner id: 12466
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: d
mutable notes: d
mutable provenance: d
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K1497011_sequence/1

BBa_K1689015
type: ComponentDefinition
persistent identity: BBa K1689015
display id: BBa_K1689015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: F[1,2]-dCas9
creator: Wei weijia
created: 2015-09-15T11:00:00Z
modified: 2015-09-27T01:33:33Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2107_
m user id: 26531
owner id: 26531
owning group id: 9
status: status/Planning
bookmark: false
mutable description: pSB1C3-6His-F[1,2]-dCas9, segment F[1,2] fusion with dCas9, 6His tag allows for protein purification.
mutable notes: the GC% is high of F[1,2]
mutable provenance: E.coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: His
sequence annotation: random spacer
sequence annotation: F[1,2]-dCas9
sequence annotation: GGGGS*2 Linker
sequence annotation: start
sequence annotation: F[1,2]
sequence annotation: dCas9
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_K1689015_sequence/1

His
type: SequenceAnnotation
persistent identity: annotation2469122
display id: annotation2469122
version: 1
top level: BBa_K1689015
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1689015/annotation2469122/range2469122/1

https://synbiohub.org/public/igem/BBa_K1689015/annotation2469122/range2469122/1
type: Range
persistent identity: range2469122
display id: range2469122
version: 1
direction: direction/forward
top level: BBa_K1689015
start: 37
end: 54
orientation: inline

random spacer
type: SequenceAnnotation
persistent identity: annotation2477781
display id: annotation2477781
version: 1
top level: BBa_K1689015
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1689015/annotation2477781/range2477781/1

https://synbiohub.org/public/igem/BBa_K1689015/annotation2477781/range2477781/1
type: Range
persistent identity: range2477781
display id: range2477781
version: 1
direction: direction/forward
top level: BBa_K1689015
start: 1
end: 33
orientation: inline

F[1,2]-dCas9
type: SequenceAnnotation
persistent identity: annotation2470142
display id: annotation2470142
version: 1
top level: BBa_K1689015
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1689015/annotation2470142/range2470142/1

https://synbiohub.org/public/igem/BBa_K1689015/annotation2470142/range2470142/1
type: Range
persistent identity: range2470142
display id: range2470142
version: 1
direction: direction/forward
top level: BBa_K1689015
start: 34
end: 4452
orientation: inline

GGGGS*2 Linker
type: SequenceAnnotation
persistent identity: annotation2470140
display id: annotation2470140
version: 1
top level: BBa_K1689015
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1689015/annotation2470140/range2470140/1

https://synbiohub.org/public/igem/BBa_K1689015/annotation2470140/range2470140/1
type: Range
persistent identity: range2470140
display id: range2470140
version: 1
direction: direction/forward
top level: BBa_K1689015
start: 316
end: 345
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2477782
display id: annotation2477782
version: 1
top level: BBa_K1689015
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1689015/annotation2477782/range2477782/1

https://synbiohub.org/public/igem/BBa_K1689015/annotation2477782/range2477782/1
type: Range
persistent identity: range2477782
display id: range2477782
version: 1
direction: direction/forward
top level: BBa_K1689015
start: 34
end: 36
orientation: inline

F[1,2]
type: SequenceAnnotation
persistent identity: annotation2469133
display id: annotation2469133
version: 1
top level: BBa_K1689015
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1689015/annotation2469133/range2469133/1

https://synbiohub.org/public/igem/BBa_K1689015/annotation2469133/range2469133/1
type: Range
persistent identity: range2469133
display id: range2469133
version: 1
direction: direction/forward
top level: BBa_K1689015
start: 55
end: 315
orientation: inline

dCas9
type: SequenceAnnotation
persistent identity: annotation2469157
display id: annotation2469157
version: 1
top level: BBa_K1689015
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1689015/annotation2469157/range2469157/1

https://synbiohub.org/public/igem/BBa_K1689015/annotation2469157/range2469157/1
type: Range
persistent identity: range2469157
display id: range2469157
version: 1
direction: direction/forward
top level: BBa_K1689015
start: 346
end: 4449
orientation: inline

random spacer
type: SequenceAnnotation
persistent identity: annotation2477784
display id: annotation2477784
version: 1
top level: BBa_K1689015
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1689015/annotation2477784/range2477784/1

https://synbiohub.org/public/igem/BBa_K1689015/annotation2477784/range2477784/1
type: Range
persistent identity: range2477784
display id: range2477784
version: 1
direction: direction/forward
top level: BBa_K1689015
start: 4453
end: 4520
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2477783
display id: annotation2477783
version: 1
top level: BBa_K1689015
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1689015/annotation2477783/range2477783/1

https://synbiohub.org/public/igem/BBa_K1689015/annotation2477783/range2477783/1
type: Range
persistent identity: range2477783
display id: range2477783
version: 1
direction: direction/forward
top level: BBa_K1689015
start: 4450
end: 4452
orientation: inline

BBa_K1640006
type: ComponentDefinition
persistent identity: BBa K1640006
display id: BBa_K1640006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: psbELJ
creator: Meghan Cook
created: 2015-09-09T11:00:00Z
modified: 2015-09-09T11:28:11Z
discontinued: false
dominant: false
group u list: _2057_
m user id: 16353
owner id: 28565
owning group id: 9
status: status/Planning
bookmark: false
mutable description: n/A
mutable notes: N/A
mutable provenance: N.A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: rbs
sequence annotation: double terminator
sequence annotation: Plac
sequence annotation: psbJ
sequence annotation: psbE
sequence annotation: psbL
sequence: https://synbiohub.org/public/igem/BBa_K1640006_sequence/1

rbs
type: SequenceAnnotation
persistent identity: annotation2448205
display id: annotation2448205
version: 1
top level: BBa_K1640006
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1640006/annotation2448205/range2448205/1

https://synbiohub.org/public/igem/BBa_K1640006/annotation2448205/range2448205/1
type: Range
persistent identity: range2448205
display id: range2448205
version: 1
direction: direction/forward
top level: BBa_K1640006
start: 43
end: 55
orientation: inline

double terminator
type: SequenceAnnotation
persistent identity: annotation2448218
display id: annotation2448218
version: 1
top level: BBa_K1640006
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1640006/annotation2448218/range2448218/1

https://synbiohub.org/public/igem/BBa_K1640006/annotation2448218/range2448218/1
type: Range
persistent identity: range2448218
display id: range2448218
version: 1
direction: direction/forward
top level: BBa_K1640006
start: 577
end: 705
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2448214
display id: annotation2448214
version: 1
top level: BBa_K1640006
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1640006/annotation2448214/range2448214/1

https://synbiohub.org/public/igem/BBa_K1640006/annotation2448214/range2448214/1
type: Range
persistent identity: range2448214
display id: range2448214
version: 1
direction: direction/forward
top level: BBa_K1640006
start: 305
end: 317
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2448216
display id: annotation2448216
version: 1
top level: BBa_K1640006
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1640006/annotation2448216/range2448216/1

https://synbiohub.org/public/igem/BBa_K1640006/annotation2448216/range2448216/1
type: Range
persistent identity: range2448216
display id: range2448216
version: 1
direction: direction/forward
top level: BBa_K1640006
start: 435
end: 447
orientation: inline

Plac
type: SequenceAnnotation
persistent identity: annotation2475062
display id: annotation2475062
version: 1
top level: BBa_K1640006
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1640006/annotation2475062/range2475062/1

https://synbiohub.org/public/igem/BBa_K1640006/annotation2475062/range2475062/1
type: Range
persistent identity: range2475062
display id: range2475062
version: 1
direction: direction/forward
top level: BBa_K1640006
start: 3
end: 42
orientation: inline

psbJ
type: SequenceAnnotation
persistent identity: annotation2448217
display id: annotation2448217
version: 1
top level: BBa_K1640006
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1640006/annotation2448217/range2448217/1

https://synbiohub.org/public/igem/BBa_K1640006/annotation2448217/range2448217/1
type: Range
persistent identity: range2448217
display id: range2448217
version: 1
direction: direction/forward
top level: BBa_K1640006
start: 448
end: 576
orientation: inline

psbE
type: SequenceAnnotation
persistent identity: annotation2448206
display id: annotation2448206
version: 1
top level: BBa_K1640006
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1640006/annotation2448206/range2448206/1

https://synbiohub.org/public/igem/BBa_K1640006/annotation2448206/range2448206/1
type: Range
persistent identity: range2448206
display id: range2448206
version: 1
direction: direction/forward
top level: BBa_K1640006
start: 56
end: 304
orientation: inline

psbL
type: SequenceAnnotation
persistent identity: annotation2448215
display id: annotation2448215
version: 1
top level: BBa_K1640006
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1640006/annotation2448215/range2448215/1

https://synbiohub.org/public/igem/BBa_K1640006/annotation2448215/range2448215/1
type: Range
persistent identity: range2448215
display id: range2448215
version: 1
direction: direction/forward
top level: BBa_K1640006
start: 318
end: 434
orientation: inline

BBa_K1763007
type: ComponentDefinition
persistent identity: BBa K1763007
display id: BBa_K1763007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Honeybee silk protein driven by T7 promoter
creator: Daniel Cancilla
created: 2015-07-22T11:00:00Z
modified: 2015-07-23T03:43:47Z
discontinued: false
dominant: false
group u list: _2185_
m user id: 18035
owner id: 18035
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This part is the Apis Mellifera (Honeybee) silk protein coding region, with a t7 promoter element upstream as well as a ribosomal binding site.
mutable notes: Keep in mind that in order for the promoter to work as expected, the plasmid must be transformed into a cell line that contains a gene coding for T7 polymerase.
mutable provenance: Genbank FJ235090.1 Genomic sequence from Apis Mellifera
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1763000
component: BBa_K525998
sequence annotation: BBa_K525998
sequence annotation: BBa_K1763000
sequence: https://synbiohub.org/public/igem/BBa_K1763007_sequence/1

BBa_K1763000
type: Component
persistent identity: component2433357
display id: component2433357
version: 1
top level: BBa_K1763007
access: public
definition: BBa_K1763000

BBa_K525998
type: Component
persistent identity: component2433354
display id: component2433354
version: 1
top level: BBa_K1763007
access: public
definition: https://synbiohub.org/public/igem/BBa_K525998/1

BBa_K525998
type: SequenceAnnotation
persistent identity: annotation2433354
display id: annotation2433354
version: 1
top level: BBa_K1763007
location: https://synbiohub.org/public/igem/BBa_K1763007/annotation2433354/range2433354/1
component: BBa_K525998

https://synbiohub.org/public/igem/BBa_K1763007/annotation2433354/range2433354/1
type: Range
persistent identity: range2433354
display id: range2433354
version: 1
direction: direction/reverse
top level: BBa_K1763007
start: 1
end: 32
orientation: inline

BBa_K1763000
type: SequenceAnnotation
persistent identity: annotation2433357
display id: annotation2433357
version: 1
top level: BBa_K1763007
location: https://synbiohub.org/public/igem/BBa_K1763007/annotation2433357/range2433357/1
component: BBa_K1763000

https://synbiohub.org/public/igem/BBa_K1763007/annotation2433357/range2433357/1
type: Range
persistent identity: range2433357
display id: range2433357
version: 1
direction: direction/reverse
top level: BBa_K1763007
start: 39
end: 989
orientation: inline

BBa_K1446002
type: ComponentDefinition
persistent identity: BBa K1446002
display id: BBa_K1446002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: superfolder GFP with N-terminal pdu-microcompartment tag
creator: Philipp Lorenz
created: 2014-09-28T11:00:00Z
modified: 2015-05-08T01:10:27Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1824_
m user id: 0
owner id: 19947
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: We have used superfolded GFP with the N-terminal pdu-microcompartment tag in order to get fluorescence images that would show localised spots of higher GFP-density, indicating that the microcompartment tag successfully inserts the attached protein into the microcompartments
mutable notes: We have designed Primers to amplify sfGFP from a plasmid, provided by Dr. Ciaran Kelly, in order to attach the microcompartment tag upstream of the sfGFP-coding region.
mutable provenance: We received a sample of sfGFP in a holding vector, provided by Dr. Ciaran Kelly.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: ribosome binding site
sequence annotation: pdu-microcompartment tag
sequence annotation: superfolder GFP coding sequence
sequence: https://synbiohub.org/public/igem/BBa_K1446002_sequence/1

ribosome binding site
type: SequenceAnnotation
persistent identity: annotation2389094
display id: annotation2389094
version: 1
top level: BBa_K1446002
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1446002/annotation2389094/range2389094/1

https://synbiohub.org/public/igem/BBa_K1446002/annotation2389094/range2389094/1
type: Range
persistent identity: range2389094
display id: range2389094
version: 1
direction: direction/forward
top level: BBa_K1446002
start: 14
end: 19
orientation: inline

pdu-microcompartment tag
type: SequenceAnnotation
persistent identity: annotation2389095
display id: annotation2389095
version: 1
top level: BBa_K1446002
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1446002/annotation2389095/range2389095/1

https://synbiohub.org/public/igem/BBa_K1446002/annotation2389095/range2389095/1
type: Range
persistent identity: range2389095
display id: range2389095
version: 1
direction: direction/forward
top level: BBa_K1446002
start: 31
end: 80
orientation: inline

superfolder GFP coding sequence
type: SequenceAnnotation
persistent identity: annotation2389096
display id: annotation2389096
version: 1
top level: BBa_K1446002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1446002/annotation2389096/range2389096/1

https://synbiohub.org/public/igem/BBa_K1446002/annotation2389096/range2389096/1
type: Range
persistent identity: range2389096
display id: range2389096
version: 1
direction: direction/forward
top level: BBa_K1446002
start: 81
end: 831
orientation: inline

BBa_K1689007
type: ComponentDefinition
persistent identity: BBa K1689007
display id: BBa_K1689007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: dCas9-C-luc
creator: ZHANG Yihao
created: 2015-09-15T11:00:00Z
modified: 2015-09-27T01:02:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2107_
m user id: 26531
owner id: 26434
owning group id: 9
status: status/Planning
bookmark: false
mutable description: the coding sequence of the fusion protein of dCas and the CFluc of the luciferase
mutable notes: Goldengate
mutable provenance: E.coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: stop codon
sequence annotation: start codon
sequence annotation: random spacer
sequence annotation: dCas9
sequence annotation: rrnB T1 terminator
sequence annotation: GGGGSGGGGS linker
sequence annotation: B0034
sequence annotation: CFluc398
sequence: https://synbiohub.org/public/igem/BBa_K1689007_sequence/1

stop codon
type: SequenceAnnotation
persistent identity: annotation2473597
display id: annotation2473597
version: 1
top level: BBa_K1689007
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1689007/annotation2473597/range2473597/1

https://synbiohub.org/public/igem/BBa_K1689007/annotation2473597/range2473597/1
type: Range
persistent identity: range2473597
display id: range2473597
version: 1
direction: direction/forward
top level: BBa_K1689007
start: 4668
end: 4670
orientation: inline

start codon
type: SequenceAnnotation
persistent identity: annotation2473593
display id: annotation2473593
version: 1
top level: BBa_K1689007
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1689007/annotation2473593/range2473593/1

https://synbiohub.org/public/igem/BBa_K1689007/annotation2473593/range2473593/1
type: Range
persistent identity: range2473593
display id: range2473593
version: 1
direction: direction/forward
top level: BBa_K1689007
start: 78
end: 80
orientation: inline

random spacer
type: SequenceAnnotation
persistent identity: annotation2477746
display id: annotation2477746
version: 1
top level: BBa_K1689007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1689007/annotation2477746/range2477746/1

https://synbiohub.org/public/igem/BBa_K1689007/annotation2477746/range2477746/1
type: Range
persistent identity: range2477746
display id: range2477746
version: 1
direction: direction/forward
top level: BBa_K1689007
start: 4765
end: 4841
orientation: inline

dCas9
type: SequenceAnnotation
persistent identity: annotation2473594
display id: annotation2473594
version: 1
top level: BBa_K1689007
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1689007/annotation2473594/range2473594/1

https://synbiohub.org/public/igem/BBa_K1689007/annotation2473594/range2473594/1
type: Range
persistent identity: range2473594
display id: range2473594
version: 1
direction: direction/forward
top level: BBa_K1689007
start: 81
end: 4181
orientation: inline

rrnB T1 terminator
type: SequenceAnnotation
persistent identity: annotation2469599
display id: annotation2469599
version: 1
top level: BBa_K1689007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1689007/annotation2469599/range2469599/1

https://synbiohub.org/public/igem/BBa_K1689007/annotation2469599/range2469599/1
type: Range
persistent identity: range2469599
display id: range2469599
version: 1
direction: direction/forward
top level: BBa_K1689007
start: 4686
end: 4764
orientation: inline

GGGGSGGGGS linker
type: SequenceAnnotation
persistent identity: annotation2473595
display id: annotation2473595
version: 1
top level: BBa_K1689007
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1689007/annotation2473595/range2473595/1

https://synbiohub.org/public/igem/BBa_K1689007/annotation2473595/range2473595/1
type: Range
persistent identity: range2473595
display id: range2473595
version: 1
direction: direction/forward
top level: BBa_K1689007
start: 4182
end: 4211
orientation: inline

random spacer
type: SequenceAnnotation
persistent identity: annotation2477747
display id: annotation2477747
version: 1
top level: BBa_K1689007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1689007/annotation2477747/range2477747/1

https://synbiohub.org/public/igem/BBa_K1689007/annotation2477747/range2477747/1
type: Range
persistent identity: range2477747
display id: range2477747
version: 1
direction: direction/forward
top level: BBa_K1689007
start: 1
end: 59
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2473592
display id: annotation2473592
version: 1
top level: BBa_K1689007
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1689007/annotation2473592/range2473592/1

https://synbiohub.org/public/igem/BBa_K1689007/annotation2473592/range2473592/1
type: Range
persistent identity: range2473592
display id: range2473592
version: 1
direction: direction/forward
top level: BBa_K1689007
start: 60
end: 77
orientation: inline

CFluc398
type: SequenceAnnotation
persistent identity: annotation2473596
display id: annotation2473596
version: 1
top level: BBa_K1689007
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1689007/annotation2473596/range2473596/1

https://synbiohub.org/public/igem/BBa_K1689007/annotation2473596/range2473596/1
type: Range
persistent identity: range2473596
display id: range2473596
version: 1
direction: direction/forward
top level: BBa_K1689007
start: 4212
end: 4667
orientation: inline

BBa_K1692003
type: ComponentDefinition
persistent identity: BBa K1692003
display id: BBa_K1692003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PAL with T7 promoter
creator: Daniel Kunin
created: 2015-08-02T11:00:00Z
modified: 2015-08-03T01:03:09Z
discontinued: false
dominant: false
group u list: _2110_
m user id: 26209
owner id: 26209
owning group id: 9
status: status/Planning
bookmark: false
mutable description: PAL with T7 promoter
mutable notes: None
mutable provenance: University British Columbia 2013 iGEM
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K525998
component: BBa_K1129003
sequence annotation: BBa_K1129003
sequence annotation: BBa_K525998
sequence: https://synbiohub.org/public/igem/BBa_K1692003_sequence/1

BBa_K525998
type: Component
persistent identity: component2433986
display id: component2433986
version: 1
top level: BBa_K1692003
access: public
definition: https://synbiohub.org/public/igem/BBa_K525998/1

BBa_K1129003
type: Component
persistent identity: component2433987
display id: component2433987
version: 1
top level: BBa_K1692003
access: public
definition: https://synbiohub.org/public/igem/BBa_K1129003/1

BBa_K1129003
type: SequenceAnnotation
persistent identity: annotation2433987
display id: annotation2433987
version: 1
top level: BBa_K1692003
location: https://synbiohub.org/public/igem/BBa_K1692003/annotation2433987/range2433987/1
component: BBa_K1129003

https://synbiohub.org/public/igem/BBa_K1692003/annotation2433987/range2433987/1
type: Range
persistent identity: range2433987
display id: range2433987
version: 1
direction: direction/reverse
top level: BBa_K1692003
start: 39
end: 1610
orientation: inline

BBa_K525998
type: SequenceAnnotation
persistent identity: annotation2433986
display id: annotation2433986
version: 1
top level: BBa_K1692003
location: https://synbiohub.org/public/igem/BBa_K1692003/annotation2433986/range2433986/1
component: BBa_K525998

https://synbiohub.org/public/igem/BBa_K1692003/annotation2433986/range2433986/1
type: Range
persistent identity: range2433986
display id: range2433986
version: 1
direction: direction/reverse
top level: BBa_K1692003
start: 1
end: 32
orientation: inline

BBa_K1393001
type: ComponentDefinition
persistent identity: BBa K1393001
display id: BBa_K1393001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: OprF(Ala196)+CBP
creator: Jiajun Tan
created: 2014-10-01T11:00:00Z
modified: 2015-07-22T02:48:08Z
discontinued: false
dominant: false
group u list: _1770_
m user id: 4206
owner id: 20256
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: The OprF is a major outer membrane protein of Pseudomonas aeruginosa. This protein functions as a nonspecific porin to allow the passage of small hydrophilic molecules, plays a structural role in maintaining cell shape and outer membrane integrity, and is required for growth under low osmolality. The structure of OprF has been proposed to consist of three domains, the N-terminal forming h-barrel structure, a loop or hinge region, and the C-terminal associated with peptidoglycan. Based on the predicted secondary structure and information found in the literature, we chose Ala196 as potential fusion sites for displaying CBP. CBP is the abbreviation of copper binding peptide made up of seven amino acid.
mutable notes: Based on the predicted secondary structure and information found in the literature, we chose Val(188) as potential fusion sites for displaying CBP. CBP is the abbreviation of copper binding peptide made up of seven amino acid.
mutable provenance: The OprF gene is from Pseudomonas aeruginosa.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: CBP
sequence annotation: oprF to Ala196
sequence: https://synbiohub.org/public/igem/BBa_K1393001_sequence/1

CBP
type: SequenceAnnotation
persistent identity: annotation2391717
display id: annotation2391717
version: 1
top level: BBa_K1393001
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1393001/annotation2391717/range2391717/1

https://synbiohub.org/public/igem/BBa_K1393001/annotation2391717/range2391717/1
type: Range
persistent identity: range2391717
display id: range2391717
version: 1
direction: direction/forward
top level: BBa_K1393001
start: 589
end: 609
orientation: inline

oprF to Ala196
type: SequenceAnnotation
persistent identity: annotation2391711
display id: annotation2391711
version: 1
top level: BBa_K1393001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1393001/annotation2391711/range2391711/1

https://synbiohub.org/public/igem/BBa_K1393001/annotation2391711/range2391711/1
type: Range
persistent identity: range2391711
display id: range2391711
version: 1
direction: direction/forward
top level: BBa_K1393001
start: 1
end: 588
orientation: inline

BBa_K1497028
type: ComponentDefinition
persistent identity: BBa K1497028
display id: BBa_K1497028
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Scaffold (P-P)
creator: Tobias Gabriel, Rene Sahm
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:45Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1877_
m user id: 0
owner id: 12466
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: d
mutable notes: d
mutable provenance: d
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1497028_sequence/1

BBa_K1585213
type: ComponentDefinition
persistent identity: BBa K1585213
display id: BBa_K1585213
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0034.XPK
creator: Laura Grabowski
created: 2015-08-24T11:00:00Z
modified: 2015-08-28T08:37:34Z
discontinued: false
dominant: false
group u list: _2002_
m user id: 19522
owner id: 24505
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This is the translational unit for the phosphoketolase (XPK). It derives from Bifidobacterium adolescentis and is codon optimized for E. coli.
mutable notes: RFC10 compatible
mutable provenance: Bifidobacterium adolescentis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1585203
component: BBa_B0034
sequence annotation: BBa_K1585203
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K1585213_sequence/1

BBa_K1585203
type: Component
persistent identity: component2439488
display id: component2439488
version: 1
top level: BBa_K1585213
access: public
definition: https://synbiohub.org/public/igem/BBa_K1585203/1

BBa_B0034
type: Component
persistent identity: component2439486
display id: component2439486
version: 1
top level: BBa_K1585213
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1585203
type: SequenceAnnotation
persistent identity: annotation2439488
display id: annotation2439488
version: 1
top level: BBa_K1585213
location: https://synbiohub.org/public/igem/BBa_K1585213/annotation2439488/range2439488/1
component: BBa_K1585203

https://synbiohub.org/public/igem/BBa_K1585213/annotation2439488/range2439488/1
type: Range
persistent identity: range2439488
display id: range2439488
version: 1
direction: direction/reverse
top level: BBa_K1585213
start: 19
end: 2499
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2439486
display id: annotation2439486
version: 1
top level: BBa_K1585213
location: https://synbiohub.org/public/igem/BBa_K1585213/annotation2439486/range2439486/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1585213/annotation2439486/range2439486/1
type: Range
persistent identity: range2439486
display id: range2439486
version: 1
direction: direction/reverse
top level: BBa_K1585213
start: 1
end: 12
orientation: inline

eYFP_SH3li
type: ComponentDefinition
persistent identity: BBa K1602038
display id: BBa_K1602038
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: eYFP with C-terminal SH3 ligand and RBS BBa_B0034
creator: Jasmin Weber, Michelle Zoeller, Marcus Geissler, Stefan Bohn, Simon Linder, Sebastian Barthel
created: 2015-09-16T11:00:00Z
modified: 2015-09-17T05:11:27Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2019_
m user id: 22022
owner id: 22022
owning group id: 9
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K1602038_sequence/1

MccS
type: ComponentDefinition
persistent identity: BBa K1659100
display id: BBa_K1659100
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Microcin S, a Class II microcin produced by probiotic E. coli G3/10
creator: Raphaella Hull
created: 2015-08-09T11:00:00Z
modified: 2016-02-03T10:51:08Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2077_
m user id: 4206
owner id: 24525
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Microcins are antibacterial peptides. Microcin S was isolated from Escherichia coli G3/10, a component of the probiotic drug Symbioflor 2. Here it was shown to be responsible for E. coli G3/10's ability to suppress adherence of enteropathogenic E. coli E2348/69. [1] The Microcin S operon is about 4.7 kb in size and is comprised of four genes. This part is the sequencing encoding the microcin itself (mcsS). [1] Zsch??ttig, A., Zimmermann, K., Blom, J., Goesmann, A., P??hlmann, C., & Gunzer, F. (2012). Identification and characterization of microcin S, a new antibacterial peptide produced by probiotic Escherichia coli G3/10. PLoS ONE, 7(3), 1???9. http://doi.org/10.1371/journal.pone.0033351
mutable notes: No specific design considerations
mutable provenance: UniProtKB - H9ZMG7 (H9ZMG7_ECOLX)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Microcin S
sequence annotation: stop
sequence annotation: start
sequence annotation: Hisx6
sequence: https://synbiohub.org/public/igem/BBa_K1659100_sequence/1

Microcin S
type: SequenceAnnotation
persistent identity: annotation2449244
display id: annotation2449244
version: 1
top level: MccS
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1659100/annotation2449244/range2449244/1

https://synbiohub.org/public/igem/BBa_K1659100/annotation2449244/range2449244/1
type: Range
persistent identity: range2449244
display id: range2449244
version: 1
direction: direction/forward
top level: MccS
start: 4
end: 360
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2449246
display id: annotation2449246
version: 1
top level: MccS
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1659100/annotation2449246/range2449246/1

https://synbiohub.org/public/igem/BBa_K1659100/annotation2449246/range2449246/1
type: Range
persistent identity: range2449246
display id: range2449246
version: 1
direction: direction/forward
top level: MccS
start: 379
end: 384
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2449243
display id: annotation2449243
version: 1
top level: MccS
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1659100/annotation2449243/range2449243/1

https://synbiohub.org/public/igem/BBa_K1659100/annotation2449243/range2449243/1
type: Range
persistent identity: range2449243
display id: range2449243
version: 1
direction: direction/forward
top level: MccS
start: 1
end: 3
orientation: inline

Hisx6
type: SequenceAnnotation
persistent identity: annotation2449245
display id: annotation2449245
version: 1
top level: MccS
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1659100/annotation2449245/range2449245/1

https://synbiohub.org/public/igem/BBa_K1659100/annotation2449245/range2449245/1
type: Range
persistent identity: range2449245
display id: range2449245
version: 1
direction: direction/forward
top level: MccS
start: 361
end: 378
orientation: inline

BBa_K1331001
type: ComponentDefinition
persistent identity: BBa K1331001
display id: BBa_K1331001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Modified rhlA coding sequence from Pseudomonas aeruginosa SQ6
creator: Tianyi Huang
created: 2014-09-24T11:00:00Z
modified: 2015-05-08T01:09:56Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1706_
m user id: 0
owner id: 20153
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: RhlA together with RhlB encodes Rhamnosyltransferase I for mono-rahmnolipids biosynthesis in Pseudomonas aeruginosa.
mutable notes: A mutation has been done to remove the PstI restriction site contained in the coding sequence.
mutable provenance: Pseudomonas aeruginosa
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: noncoding sequence
sequence annotation: rhlA
sequence: https://synbiohub.org/public/igem/BBa_K1331001_sequence/1

noncoding sequence
type: SequenceAnnotation
persistent identity: annotation2427978
display id: annotation2427978
version: 1
top level: BBa_K1331001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1331001/annotation2427978/range2427978/1

https://synbiohub.org/public/igem/BBa_K1331001/annotation2427978/range2427978/1
type: Range
persistent identity: range2427978
display id: range2427978
version: 1
direction: direction/forward
top level: BBa_K1331001
start: 889
end: 947
orientation: inline

rhlA
type: SequenceAnnotation
persistent identity: annotation2386609
display id: annotation2386609
version: 1
top level: BBa_K1331001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1331001/annotation2386609/range2386609/1

https://synbiohub.org/public/igem/BBa_K1331001/annotation2386609/range2386609/1
type: Range
persistent identity: range2386609
display id: range2386609
version: 1
direction: direction/forward
top level: BBa_K1331001
start: 1
end: 888
orientation: inline

BBa_K1763426
type: ComponentDefinition
persistent identity: BBa K1763426
display id: BBa_K1763426
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: MaSp2-6(1C3)
creator: Vinson Lam
created: 2015-09-14T11:00:00Z
modified: 2015-09-15T08:38:24Z
discontinued: false
dominant: false
group u list: _2185_
m user id: 27136
owner id: 27136
owning group id: 9
status: status/Planning
bookmark: false
mutable description: MaSp2 6 mer in pSB1C3. No regulatory elements present.
mutable notes: no
mutable provenance: Nephila clavipes
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: M2-AB
sequence annotation: M2-BC
sequence annotation: M2-CA
sequence annotation: 8-his tag
sequence: https://synbiohub.org/public/igem/BBa_K1763426_sequence/1

M2-AB
type: SequenceAnnotation
persistent identity: annotation2463835
display id: annotation2463835
version: 1
top level: BBa_K1763426
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763426/annotation2463835/range2463835/1

https://synbiohub.org/public/igem/BBa_K1763426/annotation2463835/range2463835/1
type: Range
persistent identity: range2463835
display id: range2463835
version: 1
direction: direction/forward
top level: BBa_K1763426
start: 342
end: 443
orientation: inline

M2-BC
type: SequenceAnnotation
persistent identity: annotation2463833
display id: annotation2463833
version: 1
top level: BBa_K1763426
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763426/annotation2463833/range2463833/1

https://synbiohub.org/public/igem/BBa_K1763426/annotation2463833/range2463833/1
type: Range
persistent identity: range2463833
display id: range2463833
version: 1
direction: direction/forward
top level: BBa_K1763426
start: 444
end: 545
orientation: inline

M2-AB
type: SequenceAnnotation
persistent identity: annotation2463832
display id: annotation2463832
version: 1
top level: BBa_K1763426
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763426/annotation2463832/range2463832/1

https://synbiohub.org/public/igem/BBa_K1763426/annotation2463832/range2463832/1
type: Range
persistent identity: range2463832
display id: range2463832
version: 1
direction: direction/forward
top level: BBa_K1763426
start: 36
end: 137
orientation: inline

M2-CA
type: SequenceAnnotation
persistent identity: annotation2463834
display id: annotation2463834
version: 1
top level: BBa_K1763426
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763426/annotation2463834/range2463834/1

https://synbiohub.org/public/igem/BBa_K1763426/annotation2463834/range2463834/1
type: Range
persistent identity: range2463834
display id: range2463834
version: 1
direction: direction/forward
top level: BBa_K1763426
start: 546
end: 647
orientation: inline

8-his tag
type: SequenceAnnotation
persistent identity: annotation2463831
display id: annotation2463831
version: 1
top level: BBa_K1763426
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1763426/annotation2463831/range2463831/1

https://synbiohub.org/public/igem/BBa_K1763426/annotation2463831/range2463831/1
type: Range
persistent identity: range2463831
display id: range2463831
version: 1
direction: direction/forward
top level: BBa_K1763426
start: 4
end: 27
orientation: inline

M2-CA
type: SequenceAnnotation
persistent identity: annotation2463836
display id: annotation2463836
version: 1
top level: BBa_K1763426
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763426/annotation2463836/range2463836/1

https://synbiohub.org/public/igem/BBa_K1763426/annotation2463836/range2463836/1
type: Range
persistent identity: range2463836
display id: range2463836
version: 1
direction: direction/forward
top level: BBa_K1763426
start: 240
end: 341
orientation: inline

M2-BC
type: SequenceAnnotation
persistent identity: annotation2463837
display id: annotation2463837
version: 1
top level: BBa_K1763426
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763426/annotation2463837/range2463837/1

https://synbiohub.org/public/igem/BBa_K1763426/annotation2463837/range2463837/1
type: Range
persistent identity: range2463837
display id: range2463837
version: 1
direction: direction/forward
top level: BBa_K1763426
start: 138
end: 239
orientation: inline

BBa_K1321356
type: ComponentDefinition
persistent identity: BBa K1321356
display id: BBa_K1321356
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: sfGFP fused to CBDclos driven by LacI
creator: Chris N Micklem
created: 2014-10-07T11:00:00Z
modified: 2016-01-26T09:45:38Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 4206
owner id: 20830
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: asdf
mutable notes: awef
mutable provenance: asdf
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1319106
component: BBa_K1321341
component: BBa_J04500
sequence annotation: BBa_K1319106
sequence annotation: BBa_K1321341
sequence annotation: BBa_J04500
sequence: https://synbiohub.org/public/igem/BBa_K1321356_sequence/1

BBa_K1319106
type: Component
persistent identity: component2415483
display id: component2415483
version: 1
top level: BBa_K1321356
access: public
definition: https://synbiohub.org/public/igem/BBa_K1319106/1

BBa_K1321341
type: Component
persistent identity: component2415489
display id: component2415489
version: 1
top level: BBa_K1321356
access: public
definition: BBa_K1321341

BBa_J04500
type: Component
persistent identity: component2415481
display id: component2415481
version: 1
top level: BBa_K1321356
access: public
definition: https://synbiohub.org/public/igem/BBa_J04500/1

BBa_K1319106
type: SequenceAnnotation
persistent identity: annotation2415483
display id: annotation2415483
version: 1
top level: BBa_K1321356
location: https://synbiohub.org/public/igem/BBa_K1321356/annotation2415483/range2415483/1
component: BBa_K1319106

https://synbiohub.org/public/igem/BBa_K1321356/annotation2415483/range2415483/1
type: Range
persistent identity: range2415483
display id: range2415483
version: 1
direction: direction/reverse
top level: BBa_K1321356
start: 221
end: 235
orientation: inline

BBa_K1321341
type: SequenceAnnotation
persistent identity: annotation2415489
display id: annotation2415489
version: 1
top level: BBa_K1321356
location: https://synbiohub.org/public/igem/BBa_K1321356/annotation2415489/range2415489/1
component: BBa_K1321341

https://synbiohub.org/public/igem/BBa_K1321356/annotation2415489/range2415489/1
type: Range
persistent identity: range2415489
display id: range2415489
version: 1
direction: direction/reverse
top level: BBa_K1321356
start: 236
end: 1252
orientation: inline

BBa_J04500
type: SequenceAnnotation
persistent identity: annotation2415481
display id: annotation2415481
version: 1
top level: BBa_K1321356
location: https://synbiohub.org/public/igem/BBa_K1321356/annotation2415481/range2415481/1
component: BBa_J04500

https://synbiohub.org/public/igem/BBa_K1321356/annotation2415481/range2415481/1
type: Range
persistent identity: range2415481
display id: range2415481
version: 1
direction: direction/reverse
top level: BBa_K1321356
start: 1
end: 220
orientation: inline

BBa_K1325007
type: ComponentDefinition
persistent identity: BBa K1325007
display id: BBa_K1325007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: MinE
creator: Ke Xu
created: 2014-09-28T11:00:00Z
modified: 2015-05-08T01:09:54Z
discontinued: false
dominant: false
group u list: _1700_
m user id: 0
owner id: 23253
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: cell division topological specificity factor MinE
mutable notes: no
mutable provenance: E.coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1325007_sequence/1

BBa_K1321094
type: ComponentDefinition
persistent identity: BBa K1321094
display id: BBa_K1321094
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CBDclos fused to Phytochelatin (PC) EC20
creator: Xenia Spencer-Milnes
created: 2014-10-06T11:00:00Z
modified: 2015-06-17T12:17:50Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 4206
owner id: 20780
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: CBD4+PhytoC. TBC
mutable notes: CBD4+PhytoC. TBC
mutable provenance: CBD4+PhytoC. TBC
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1321002
component: BBa_B0105
component: BBa_K1321005
sequence annotation: BBa_K1321002
sequence annotation: BBa_B0105
sequence annotation: BBa_K1321005
sequence: https://synbiohub.org/public/igem/BBa_K1321094_sequence/1

BBa_K1321002
type: Component
persistent identity: component2414631
display id: component2414631
version: 1
top level: BBa_K1321094
access: public
definition: https://synbiohub.org/public/igem/BBa_K1321002/1

BBa_B0105
type: Component
persistent identity: component2414633
display id: component2414633
version: 1
top level: BBa_K1321094
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_K1321005
type: Component
persistent identity: component2414635
display id: component2414635
version: 1
top level: BBa_K1321094
access: public
definition: BBa_K1321005

BBa_K1321002
type: SequenceAnnotation
persistent identity: annotation2414631
display id: annotation2414631
version: 1
top level: BBa_K1321094
location: https://synbiohub.org/public/igem/BBa_K1321094/annotation2414631/range2414631/1
component: BBa_K1321002

https://synbiohub.org/public/igem/BBa_K1321094/annotation2414631/range2414631/1
type: Range
persistent identity: range2414631
display id: range2414631
version: 1
direction: direction/reverse
top level: BBa_K1321094
start: 1
end: 300
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2414633
display id: annotation2414633
version: 1
top level: BBa_K1321094
location: https://synbiohub.org/public/igem/BBa_K1321094/annotation2414633/range2414633/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_K1321094/annotation2414633/range2414633/1
type: Range
persistent identity: range2414633
display id: range2414633
version: 1
direction: direction/reverse
top level: BBa_K1321094
start: 301
end: 306
orientation: inline

BBa_K1321005
type: SequenceAnnotation
persistent identity: annotation2414635
display id: annotation2414635
version: 1
top level: BBa_K1321094
location: https://synbiohub.org/public/igem/BBa_K1321094/annotation2414635/range2414635/1
component: BBa_K1321005

https://synbiohub.org/public/igem/BBa_K1321094/annotation2414635/range2414635/1
type: Range
persistent identity: range2414635
display id: range2414635
version: 1
direction: direction/reverse
top level: BBa_K1321094
start: 307
end: 429
orientation: inline

SAMmt
type: ComponentDefinition
persistent identity: BBa K1312005
display id: BBa_K1312005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: S-adenosyl methionine-dependent methyltransferase relate to Met-DMSP byosynthesis pathway(SAMmt)
creator: Yutaka Murata
created: 2014-10-04T11:00:00Z
modified: 2015-05-08T01:09:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1687_
m user id: 0
owner id: 17633
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part is S-adenosyl methionine-dependent methyltransferase gene of Fragilariopsis cylindrus. The gene code S-adenosyl methionine-dependent methyltransferase relate to Met-DMSP byosynthesis pathway from Fragilariopsis cylindrus. which catalyze third step of Met-DMSP biosynthesis pathway.
mutable notes: This part was excluded intron. And there was PstⅠ recognition site so I made single base substitution in recognition site.
mutable provenance: From Fragilariopsis cylindrus.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: SAMmt
sequence: https://synbiohub.org/public/igem/BBa_K1312005_sequence/1

SAMmt
type: SequenceAnnotation
persistent identity: annotation2428845
display id: annotation2428845
version: 1
top level: SAMmt
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1312005/annotation2428845/range2428845/1

https://synbiohub.org/public/igem/BBa_K1312005/annotation2428845/range2428845/1
type: Range
persistent identity: range2428845
display id: range2428845
version: 1
direction: direction/forward
top level: SAMmt
start: 1
end: 1315
orientation: inline

RBS-xylB
type: ComponentDefinition
persistent identity: BBa K1602000
display id: BBa_K1602000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0034-xylB
creator: Jonas Sindlinger, Leon Kraus, Jonathan Pletzer-Zelgert, Elena Tonchevska, Sebastian Holzner, Mirjam Haun, Sebastian Barthel
created: 2015-08-27T11:00:00Z
modified: 2015-09-25T01:14:47Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2019_
m user id: 12512
owner id: 25989
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: BBa_IA Itaconic acid is an organic, dicarboxylic acid that is biotechnologically synthesized in Aspergillus terreus. It is derived from citric acid via 2 intermediates and a final decarboxylation. To enable this pathway in Escherichia coli it is necessary to introduce 3 genes. 2 of which are already established in the citric acid cycle and one that is from Apergillus terreus, a cis-aconitate decarboxylase (cadA). Figure 1 Reaction scheme of the itaconic acid producing operon. The substrates for the reaction are acetyl-coa and oxaloacetate. To simplify the process we are only looking at oxaloacetate right now. Oxaloacetate is metabolized to itaconic acid in 3 steps. ===Usage=== This part is a composite of three genes, each with a strong RBS (BBa_B0034) and all of them under control of one T7 Promoter (BBa_K1497017). citrate synthase - gltA (BBa_K1033001) aconitate hydratase 1 - acnA (BBa_K1033000) cis-aconitate decarboxylase - cadA (BBa_K1497100) iGEM TU Darmstadt 2014 :) Figure 2 Genetic map of the itaconic acid producing operon with T7 promoter. This brick directs the flux towards and finally enables E.Coli BL21 cells to synthesize itaconic acid in presence of the inductor IPTG.
mutable notes: BLANK
mutable provenance: GenBank
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_B0034
component: BBa_K1602009
sequence annotation: BBa_K1602009
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K1602000_sequence/1

BBa_B0034
type: Component
persistent identity: component2463049
display id: component2463049
version: 1
top level: RBS-xylB
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1602009
type: Component
persistent identity: component2463050
display id: component2463050
version: 1
top level: RBS-xylB
access: public
definition: BBa_K1602009

BBa_K1602009
type: SequenceAnnotation
persistent identity: annotation2463050
display id: annotation2463050
version: 1
top level: RBS-xylB
location: https://synbiohub.org/public/igem/BBa_K1602000/annotation2463050/range2463050/1
component: BBa_K1602009

https://synbiohub.org/public/igem/BBa_K1602000/annotation2463050/range2463050/1
type: Range
persistent identity: range2463050
display id: range2463050
version: 1
direction: direction/reverse
top level: RBS-xylB
start: 19
end: 765
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2463049
display id: annotation2463049
version: 1
top level: RBS-xylB
location: https://synbiohub.org/public/igem/BBa_K1602000/annotation2463049/range2463049/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1602000/annotation2463049/range2463049/1
type: Range
persistent identity: range2463049
display id: range2463049
version: 1
direction: direction/reverse
top level: RBS-xylB
start: 1
end: 12
orientation: inline

N-carrier
type: ComponentDefinition
persistent identity: BBa K1362053
display id: BBa_K1362053
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Carrier for RFC[105] N-terminal inserts
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B??scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch??fer, Carolin Schmelas, Silvan Schmitz, Max Waldha
created: 2014-10-07T11:00:00Z
modified: 2015-05-08T01:10:04Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1738_
m user id: 0
owner id: 22911
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
component: BBa_K1362423
component: BBa_K1362436
component: BBa_K1362440
component: BBa_K1362415
component: BBa_K1362426
component: BBa_K1362425
sequence annotation: BBa_K1362436
sequence annotation: BBa_K1362423
sequence annotation: BBa_K1362440
sequence annotation: BBa_K1362415
sequence annotation: BBa_K1362426
sequence annotation: BBa_K1362425
sequence: https://synbiohub.org/public/igem/BBa_K1362053_sequence/1

BBa_K1362423
type: Component
persistent identity: component2409689
display id: component2409689
version: 1
top level: N-carrier
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362423/1

BBa_K1362436
type: Component
persistent identity: component2409687
display id: component2409687
version: 1
top level: N-carrier
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362436/1

BBa_K1362440
type: Component
persistent identity: component2409686
display id: component2409686
version: 1
top level: N-carrier
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362440/1

BBa_K1362415
type: Component
persistent identity: component2409688
display id: component2409688
version: 1
top level: N-carrier
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362415/1

BBa_K1362426
type: Component
persistent identity: component2409690
display id: component2409690
version: 1
top level: N-carrier
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362426/1

BBa_K1362425
type: Component
persistent identity: component2409685
display id: component2409685
version: 1
top level: N-carrier
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362425/1

BBa_K1362436
type: SequenceAnnotation
persistent identity: annotation2409687
display id: annotation2409687
version: 1
top level: N-carrier
location: https://synbiohub.org/public/igem/BBa_K1362053/annotation2409687/range2409687/1
component: BBa_K1362436

https://synbiohub.org/public/igem/BBa_K1362053/annotation2409687/range2409687/1
type: Range
persistent identity: range2409687
display id: range2409687
version: 1
direction: direction/reverse
top level: N-carrier
start: 11
end: 16
orientation: inline

BBa_K1362423
type: SequenceAnnotation
persistent identity: annotation2409689
display id: annotation2409689
version: 1
top level: N-carrier
location: https://synbiohub.org/public/igem/BBa_K1362053/annotation2409689/range2409689/1
component: BBa_K1362423

https://synbiohub.org/public/igem/BBa_K1362053/annotation2409689/range2409689/1
type: Range
persistent identity: range2409689
display id: range2409689
version: 1
direction: direction/reverse
top level: N-carrier
start: 21
end: 27
orientation: inline

BBa_K1362440
type: SequenceAnnotation
persistent identity: annotation2409686
display id: annotation2409686
version: 1
top level: N-carrier
location: https://synbiohub.org/public/igem/BBa_K1362053/annotation2409686/range2409686/1
component: BBa_K1362440

https://synbiohub.org/public/igem/BBa_K1362053/annotation2409686/range2409686/1
type: Range
persistent identity: range2409686
display id: range2409686
version: 1
direction: direction/reverse
top level: N-carrier
start: 7
end: 10
orientation: inline

BBa_K1362415
type: SequenceAnnotation
persistent identity: annotation2409688
display id: annotation2409688
version: 1
top level: N-carrier
location: https://synbiohub.org/public/igem/BBa_K1362053/annotation2409688/range2409688/1
component: BBa_K1362415

https://synbiohub.org/public/igem/BBa_K1362053/annotation2409688/range2409688/1
type: Range
persistent identity: range2409688
display id: range2409688
version: 1
direction: direction/reverse
top level: N-carrier
start: 17
end: 20
orientation: inline

BBa_K1362426
type: SequenceAnnotation
persistent identity: annotation2409690
display id: annotation2409690
version: 1
top level: N-carrier
location: https://synbiohub.org/public/igem/BBa_K1362053/annotation2409690/range2409690/1
component: BBa_K1362426

https://synbiohub.org/public/igem/BBa_K1362053/annotation2409690/range2409690/1
type: Range
persistent identity: range2409690
display id: range2409690
version: 1
direction: direction/reverse
top level: N-carrier
start: 28
end: 31
orientation: inline

BBa_K1362425
type: SequenceAnnotation
persistent identity: annotation2409685
display id: annotation2409685
version: 1
top level: N-carrier
location: https://synbiohub.org/public/igem/BBa_K1362053/annotation2409685/range2409685/1
component: BBa_K1362425

https://synbiohub.org/public/igem/BBa_K1362053/annotation2409685/range2409685/1
type: Range
persistent identity: range2409685
display id: range2409685
version: 1
direction: direction/reverse
top level: N-carrier
start: 1
end: 6
orientation: inline

BBa_K1351021
type: ComponentDefinition
persistent identity: BBa K1351021
display id: BBa_K1351021
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Monomeric Red Fluorescent Protein from Discosoma striata
creator: Anna Sommer
created: 2014-09-29T11:00:00Z
modified: 2016-01-26T01:58:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1726_
m user id: 4206
owner id: 20043
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: Mutant of the Biobrick BBa_E1010, in order to make it compatible with the Freiburg-Standard, two AgeI-Restriction sites were deleted monomeric RFP: Red Fluorescent Protein. Excitation peak: 584 nm Emission peak: 607 nm
mutable notes: The Biobrick has the Following Prefix and Suffix: Gatcgaattccgcggccgcttctagataaggaggagccggc Gatcctgcagcggccgctactagtattaaccggt
mutable provenance: Discosoma striata (coral)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1351021_sequence/1

BBa_K1789000
type: ComponentDefinition
persistent identity: BBa K1789000
display id: BBa_K1789000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: IaaM
creator: Xinyuan Qiu
created: 2015-09-11T11:00:00Z
modified: 2015-09-12T02:27:07Z
discontinued: false
dominant: false
group u list: _2214_
m user id: 21134
owner id: 21134
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This part is the coding sequence of tryptophan-2-mono-oxygenase. This enzyme catalyzes the oxidative carboxylation of L-tryptophan to indole-3-acetamide which acts as an intermediate to the production of indole-3-acetic acid in the IAM pathway.
mutable notes: The reason why er designed this part is that we needed IAA producing pathway as a reporter in our system, and the IAAM and IAAH CDS needed to be fused with the coding sequence of the TAL effector we engineered. So we used the part BBa_K515100, which contains the CDS of IaaM and IaaH, and used PCR to eliminate the RBS sequence from the 5' region of the CDS.
mutable provenance: This part is gained from BBa_K515100 by PCR. The IAA producing pathway originates from Pseudomonas savastanoi.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: IaaM
sequence: https://synbiohub.org/public/igem/BBa_K1789000_sequence/1

IaaM
type: SequenceAnnotation
persistent identity: annotation2452420
display id: annotation2452420
version: 1
top level: BBa_K1789000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1789000/annotation2452420/range2452420/1

https://synbiohub.org/public/igem/BBa_K1789000/annotation2452420/range2452420/1
type: Range
persistent identity: range2452420
display id: range2452420
version: 1
direction: direction/forward
top level: BBa_K1789000
start: 1
end: 1638
orientation: inline

BBa_K1763423
type: ComponentDefinition
persistent identity: BBa K1763423
display id: BBa_K1763423
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: MaSp1-SeqAB2
creator: Vinson Lam
created: 2015-09-14T11:00:00Z
modified: 2016-02-10T11:27:38Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2185_
m user id: 4206
owner id: 27136
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This part contains the core sequence of MaSp2 that has been assembled with a 5'-AGTT-3' overhang on the 5' end of the sequence and 3'-ACAG-5' overhang on the 3' end of the sequence. This part was designed for use with ICA, along with parts BBa_K1763003 and BBa_K1763004 as an efficient method to assemble multiple MaSp2 sequences together. This setup is to show that this particular set of sticky ends is suitable for ICA. Before using in ICA, this part should be digested with BsaI, a type IIs restriction enzyme, which cuts DNA outside of its recognition site. Sequence and Features
mutable notes: The gene sequence for MaSp2 is particularly GC-rich, which may complicate PCR.
mutable provenance: This part is derived from the MaSp2 gene found in the spider N. Clavipes.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: B sticky end
sequence annotation: BsaI recognition site (forward)
sequence annotation: BsaI recognition site (reverse)
sequence annotation: MaSp1 SeqR Primer
sequence annotation: A sticky end
sequence annotation: MaSp1 Core Sequence
sequence annotation: MaSp1 SeqF Primer
sequence: https://synbiohub.org/public/igem/BBa_K1763423_sequence/1

B sticky end
type: SequenceAnnotation
persistent identity: annotation2463793
display id: annotation2463793
version: 1
top level: BBa_K1763423
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1763423/annotation2463793/range2463793/1

https://synbiohub.org/public/igem/BBa_K1763423/annotation2463793/range2463793/1
type: Range
persistent identity: range2463793
display id: range2463793
version: 1
direction: direction/forward
top level: BBa_K1763423
start: 109
end: 112
orientation: inline

BsaI recognition site (forward)
type: SequenceAnnotation
persistent identity: annotation2463789
display id: annotation2463789
version: 1
top level: BBa_K1763423
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1763423/annotation2463789/range2463789/1

https://synbiohub.org/public/igem/BBa_K1763423/annotation2463789/range2463789/1
type: Range
persistent identity: range2463789
display id: range2463789
version: 1
direction: direction/forward
top level: BBa_K1763423
start: 1
end: 5
orientation: inline

BsaI recognition site (reverse)
type: SequenceAnnotation
persistent identity: annotation2463790
display id: annotation2463790
version: 1
top level: BBa_K1763423
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1763423/annotation2463790/range2463790/1

https://synbiohub.org/public/igem/BBa_K1763423/annotation2463790/range2463790/1
type: Range
persistent identity: range2463790
display id: range2463790
version: 1
direction: direction/reverse
top level: BBa_K1763423
start: 114
end: 119
orientation: inline

MaSp1 SeqR Primer
type: SequenceAnnotation
persistent identity: annotation2473474
display id: annotation2473474
version: 1
top level: BBa_K1763423
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K1763423/annotation2473474/range2473474/1

https://synbiohub.org/public/igem/BBa_K1763423/annotation2473474/range2473474/1
type: Range
persistent identity: range2473474
display id: range2473474
version: 1
direction: direction/reverse
top level: BBa_K1763423
start: 41
end: 58
orientation: inline

A sticky end
type: SequenceAnnotation
persistent identity: annotation2463791
display id: annotation2463791
version: 1
top level: BBa_K1763423
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1763423/annotation2463791/range2463791/1

https://synbiohub.org/public/igem/BBa_K1763423/annotation2463791/range2463791/1
type: Range
persistent identity: range2463791
display id: range2463791
version: 1
direction: direction/forward
top level: BBa_K1763423
start: 7
end: 10
orientation: inline

MaSp1 Core Sequence
type: SequenceAnnotation
persistent identity: annotation2473472
display id: annotation2473472
version: 1
top level: BBa_K1763423
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763423/annotation2473472/range2473472/1

https://synbiohub.org/public/igem/BBa_K1763423/annotation2473472/range2473472/1
type: Range
persistent identity: range2473472
display id: range2473472
version: 1
direction: direction/forward
top level: BBa_K1763423
start: 11
end: 108
orientation: inline

MaSp1 SeqF Primer
type: SequenceAnnotation
persistent identity: annotation2473473
display id: annotation2473473
version: 1
top level: BBa_K1763423
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K1763423/annotation2473473/range2473473/1

https://synbiohub.org/public/igem/BBa_K1763423/annotation2473473/range2473473/1
type: Range
persistent identity: range2473473
display id: range2473473
version: 1
direction: direction/forward
top level: BBa_K1763423
start: 41
end: 58
orientation: inline

Tamura
type: ComponentDefinition
persistent identity: BBa K1763444
display id: BBa_K1763444
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Bombyx mori silk co-spinning module
creator: Fasih Ahsan
created: 2015-09-14T11:00:00Z
modified: 2016-02-10T11:26:30Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2185_
m user id: 4206
owner id: 22156
owning group id: 9
status: status/Planning
bookmark: true
mutable description: Studies investigating the potential for co-spinning, a process in which the terminal domains of the functional B. mori silk protein heavy chain are fused with genes encoding functional binding domains, and subsequently are combined with native silk proteins to form crystalline fibers, have shown some success (Sponner et al., 2005). In vivo co-spinning simulations have previously been conducted using the transgene in silkworms, producing functionally fluorescent N-terminal ??? GFP- C-terminal fusions that successfully incorporate into endogenous Bombyx mori silkworm silk monomers (Kojima et. al., 2007). These N-Terminal and C-terminal regions are not only easier to clone due to their non-repetitive structures, but additionally allows for storage and assembly of endogenous ampullate spidroins (Askarieh et al., 2010). Composite silkworm-spider silk chimeras have shown improved mechanical properties, and may increase the ease in high-yield production due to the easier manipulation of silkworm silk monomers (Teule, et. al. 2012). Yet, this process still relies on the use of transgenic silkworms, which have longer developmental cycles, require larger storage and nutrient conditions, and difficulty to harvest relative to bacterial counterparts (Murphy et al., 2009). An ex situ co-spinning process has been developed for the production of composite N. clavipes ??? B. mori silk fibers with functional protein fusions in bacterial chassis (namely, E. coli). This process results in the construction of a transgene that can be modularized using restriction enzymes to incorporate a wide range of functional peptide binding sites or other proteins, including avidin-biotin binding domains, RGD-cadherin motifs, albumin binding domains, or antibody affinity domains (Jansson et al., 2014). Specifically, this composite structure focuses on the ability to express functional sfGFP in silk fibers as a marker for successful co-spinning, and verifies proper NC-sfGFP expression using SDS-PAGE a…
mutable notes: We decided to use a LacI promoter to regulate expression of the large protein construct in E. coli K12 laboratory strains, in lieu of utilizing a T7 promoter or constitutive Anderson family promoters. This is to maximize the protein yield of the co-spinning module without causing a tradeoff between regular cell growth and housekeeping protein synthesis. Additionally, we decided to use sfGFP as the reporter gene, instead of a standard mut3b GFP, due to the ability of sfGFP to function even when fused to large bulky protein domains (in this case, FibNT and FibCT). Lastly, we designed BsaI TypeIIS restriction sites flanking the reporter gene that when cut, generates sticky ends compatible with EcoRI and SpeI sites. This was created to allow iGEM teams to take protein domains of interest from the Standard Registry of Parts, and fuse them into the co-spinning module by a simple Digest and Ligate assembly to swap the sfGFP construct. However, the protein domain to be added must have the Silver Fusion prefix and suffix, to allow for in-frame cloning of the protein domain. Without this in-frame cloning, the scar sites of the tradition prefix and suffix will cause a frameshift mutation that mutate the translation product, and renders it non-functional.
mutable provenance: The LacI promoter (BBa_R0010), RBS (BBa_B0034), and super folder GFP (BBa_I746916) were derived from sequences found in the Standard Registry of Parts. The Bombyx mori silk fibroin heavy chain N-terminal (FibNT) and C-terminal (FibCT) domains were derived from the sequenced consensus genome of Bombyx mori silkworm species, and were synthesized using IDT gBlocks gene fragments.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: Bba prefix/suffix assembly scar site
sequence annotation: LacI (BBa_R0010)
sequence annotation: Ribosome Double-Stop Site
sequence annotation: superfolder GFP (BBa_I746916)
sequence annotation: BsaI Restriction Site (EcoRI Compatible)
sequence annotation: Elowitz RBS (BBa_B0034)
sequence annotation: B. mori FibNT
sequence annotation: B. mori FibCT
sequence annotation: 6x N-terminal polyhistidine tag
sequence: https://synbiohub.org/public/igem/BBa_K1763444_sequence/1

Bba prefix/suffix assembly scar site
type: SequenceAnnotation
persistent identity: annotation2462433
display id: annotation2462433
version: 1
top level: Tamura
role: feature/scar
location: https://synbiohub.org/public/igem/BBa_K1763444/annotation2462433/range2462433/1

https://synbiohub.org/public/igem/BBa_K1763444/annotation2462433/range2462433/1
type: Range
persistent identity: range2462433
display id: range2462433
version: 1
direction: direction/forward
top level: Tamura
start: 201
end: 208
orientation: inline

LacI (BBa_R0010)
type: SequenceAnnotation
persistent identity: annotation2462432
display id: annotation2462432
version: 1
top level: Tamura
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1763444/annotation2462432/range2462432/1

https://synbiohub.org/public/igem/BBa_K1763444/annotation2462432/range2462432/1
type: Range
persistent identity: range2462432
display id: range2462432
version: 1
direction: direction/forward
top level: Tamura
start: 1
end: 200
orientation: inline

Ribosome Double-Stop Site
type: SequenceAnnotation
persistent identity: annotation2462440
display id: annotation2462440
version: 1
top level: Tamura
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1763444/annotation2462440/range2462440/1

https://synbiohub.org/public/igem/BBa_K1763444/annotation2462440/range2462440/1
type: Range
persistent identity: range2462440
display id: range2462440
version: 1
direction: direction/forward
top level: Tamura
start: 1628
end: 1633
orientation: inline

superfolder GFP (BBa_I746916)
type: SequenceAnnotation
persistent identity: annotation2462438
display id: annotation2462438
version: 1
top level: Tamura
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1763444/annotation2462438/range2462438/1

https://synbiohub.org/public/igem/BBa_K1763444/annotation2462438/range2462438/1
type: Range
persistent identity: range2462438
display id: range2462438
version: 1
direction: direction/forward
top level: Tamura
start: 725
end: 1438
orientation: inline

BsaI Restriction Site (EcoRI Compatible)
type: SequenceAnnotation
persistent identity: annotation2462437
display id: annotation2462437
version: 1
top level: Tamura
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763444/annotation2462437/range2462437/1

https://synbiohub.org/public/igem/BBa_K1763444/annotation2462437/range2462437/1
type: Range
persistent identity: range2462437
display id: range2462437
version: 1
direction: direction/forward
top level: Tamura
start: 719
end: 724
orientation: inline

Elowitz RBS (BBa_B0034)
type: SequenceAnnotation
persistent identity: annotation2462434
display id: annotation2462434
version: 1
top level: Tamura
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1763444/annotation2462434/range2462434/1

https://synbiohub.org/public/igem/BBa_K1763444/annotation2462434/range2462434/1
type: Range
persistent identity: range2462434
display id: range2462434
version: 1
direction: direction/forward
top level: Tamura
start: 209
end: 220
orientation: inline

B. mori FibNT
type: SequenceAnnotation
persistent identity: annotation2462436
display id: annotation2462436
version: 1
top level: Tamura
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1763444/annotation2462436/range2462436/1

https://synbiohub.org/public/igem/BBa_K1763444/annotation2462436/range2462436/1
type: Range
persistent identity: range2462436
display id: range2462436
version: 1
direction: direction/forward
top level: Tamura
start: 248
end: 712
orientation: inline

B. mori FibCT
type: SequenceAnnotation
persistent identity: annotation2462439
display id: annotation2462439
version: 1
top level: Tamura
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1763444/annotation2462439/range2462439/1

https://synbiohub.org/public/igem/BBa_K1763444/annotation2462439/range2462439/1
type: Range
persistent identity: range2462439
display id: range2462439
version: 1
direction: direction/forward
top level: Tamura
start: 1451
end: 1627
orientation: inline

6x N-terminal polyhistidine tag
type: SequenceAnnotation
persistent identity: annotation2462435
display id: annotation2462435
version: 1
top level: Tamura
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1763444/annotation2462435/range2462435/1

https://synbiohub.org/public/igem/BBa_K1763444/annotation2462435/range2462435/1
type: Range
persistent identity: range2462435
display id: range2462435
version: 1
direction: direction/forward
top level: Tamura
start: 230
end: 247
orientation: inline

BBa_K1789002
type: ComponentDefinition
persistent identity: BBa K1789002
display id: BBa_K1789002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS30
creator: Xinyuan Qiu
created: 2015-09-13T11:00:00Z
modified: 2015-09-14T06:03:14Z
discontinued: false
dominant: false
group u list: _2214_
m user id: 21134
owner id: 21134
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This part is another copy of the RBS30 (BBa_B0030). It is a Strong RBS based on Ron Weiss thesis.
mutable notes: The reason why we built this part by ourselves is that the copy of B0030 in the 2015 DNA distribution we received is sequenced as incorrect. And same problem also occurred in several other teams.
mutable provenance: The part sequence was gained from the registry, the part is annealed from the ssDNA synthesized by Life(R).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS30
sequence: https://synbiohub.org/public/igem/BBa_K1789002_sequence/1

RBS30
type: SequenceAnnotation
persistent identity: annotation2454064
display id: annotation2454064
version: 1
top level: BBa_K1789002
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1789002/annotation2454064/range2454064/1

https://synbiohub.org/public/igem/BBa_K1789002/annotation2454064/range2454064/1
type: Range
persistent identity: range2454064
display id: range2454064
version: 1
direction: direction/forward
top level: BBa_K1789002
start: 1
end: 15
orientation: inline

BBa_K1321340
type: ComponentDefinition
persistent identity: BBa K1321340
display id: BBa_K1321340
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Double CBD (dCBD) with N-terminal linker
creator: Chris N Micklem
created: 2014-10-07T11:00:00Z
modified: 2015-06-17T12:14:46Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 4206
owner id: 20830
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: dCBD with N-terminal linker.
mutable notes: asdf
mutable provenance: asdf
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: cbh1 CBD
sequence annotation: Linker
sequence annotation: cbh2 CBD
sequence annotation: dCBD
sequence: https://synbiohub.org/public/igem/BBa_K1321340_sequence/1

cbh1 CBD
type: SequenceAnnotation
persistent identity: annotation2417851
display id: annotation2417851
version: 1
top level: BBa_K1321340
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1321340/annotation2417851/range2417851/1

https://synbiohub.org/public/igem/BBa_K1321340/annotation2417851/range2417851/1
type: Range
persistent identity: range2417851
display id: range2417851
version: 1
direction: direction/forward
top level: BBa_K1321340
start: 259
end: 366
orientation: inline

Linker
type: SequenceAnnotation
persistent identity: annotation2417848
display id: annotation2417848
version: 1
top level: BBa_K1321340
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1321340/annotation2417848/range2417848/1

https://synbiohub.org/public/igem/BBa_K1321340/annotation2417848/range2417848/1
type: Range
persistent identity: range2417848
display id: range2417848
version: 1
direction: direction/forward
top level: BBa_K1321340
start: 1
end: 72
orientation: inline

cbh2 CBD
type: SequenceAnnotation
persistent identity: annotation2417849
display id: annotation2417849
version: 1
top level: BBa_K1321340
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1321340/annotation2417849/range2417849/1

https://synbiohub.org/public/igem/BBa_K1321340/annotation2417849/range2417849/1
type: Range
persistent identity: range2417849
display id: range2417849
version: 1
direction: direction/forward
top level: BBa_K1321340
start: 73
end: 186
orientation: inline

dCBD
type: SequenceAnnotation
persistent identity: annotation2417847
display id: annotation2417847
version: 1
top level: BBa_K1321340
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1321340/annotation2417847/range2417847/1

https://synbiohub.org/public/igem/BBa_K1321340/annotation2417847/range2417847/1
type: Range
persistent identity: range2417847
display id: range2417847
version: 1
direction: direction/forward
top level: BBa_K1321340
start: 1
end: 366
orientation: inline

Linker
type: SequenceAnnotation
persistent identity: annotation2417850
display id: annotation2417850
version: 1
top level: BBa_K1321340
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1321340/annotation2417850/range2417850/1

https://synbiohub.org/public/igem/BBa_K1321340/annotation2417850/range2417850/1
type: Range
persistent identity: range2417850
display id: range2417850
version: 1
direction: direction/forward
top level: BBa_K1321340
start: 187
end: 258
orientation: inline

BBa_J04450
type: ComponentDefinition
persistent identity: BBa J04450
display id: BBa_J04450
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RFP Coding Device
creator: Tamar Odle
created: 2005-06-08T11:00:00Z
modified: 2015-08-31T04:08:14Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _16_
m user id: 0
owner id: 328
owning group id: 16
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Contains an IPTG inducible promoter an RBS, RFP, and terminator.
mutable provenance: Davidson Synth-Aces
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_E1010
component: BBa_B0010
component: BBa_R0010
component: BBa_B0012
component: BBa_B0034
sequence annotation: BBa_B0010
sequence annotation: BBa_R0010
sequence annotation: BBa_B0012
sequence annotation: BBa_E1010
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_J04450_sequence/1

BBa_E1010
type: Component
persistent identity: component1509411
display id: component1509411
version: 1
top level: BBa_J04450
access: public
definition: https://synbiohub.org/public/igem/BBa_E1010/1

BBa_B0010
type: Component
persistent identity: component1509417
display id: component1509417
version: 1
top level: BBa_J04450
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_R0010
type: Component
persistent identity: component1509394
display id: component1509394
version: 1
top level: BBa_J04450
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_B0012
type: Component
persistent identity: component1509427
display id: component1509427
version: 1
top level: BBa_J04450
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
type: Component
persistent identity: component1509404
display id: component1509404
version: 1
top level: BBa_J04450
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1509417
display id: annotation1509417
version: 1
top level: BBa_J04450
location: https://synbiohub.org/public/igem/BBa_J04450/annotation1509417/range1509417/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J04450/annotation1509417/range1509417/1
type: Range
persistent identity: range1509417
display id: range1509417
version: 1
direction: direction/forward
top level: BBa_J04450
start: 941
end: 1020
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1509394
display id: annotation1509394
version: 1
top level: BBa_J04450
location: https://synbiohub.org/public/igem/BBa_J04450/annotation1509394/range1509394/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_J04450/annotation1509394/range1509394/1
type: Range
persistent identity: range1509394
display id: range1509394
version: 1
direction: direction/forward
top level: BBa_J04450
start: 1
end: 200
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1509427
display id: annotation1509427
version: 1
top level: BBa_J04450
location: https://synbiohub.org/public/igem/BBa_J04450/annotation1509427/range1509427/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J04450/annotation1509427/range1509427/1
type: Range
persistent identity: range1509427
display id: range1509427
version: 1
direction: direction/forward
top level: BBa_J04450
start: 1029
end: 1069
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation1509411
display id: annotation1509411
version: 1
top level: BBa_J04450
location: https://synbiohub.org/public/igem/BBa_J04450/annotation1509411/range1509411/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_J04450/annotation1509411/range1509411/1
type: Range
persistent identity: range1509411
display id: range1509411
version: 1
direction: direction/forward
top level: BBa_J04450
start: 227
end: 907
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1509404
display id: annotation1509404
version: 1
top level: BBa_J04450
location: https://synbiohub.org/public/igem/BBa_J04450/annotation1509404/range1509404/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J04450/annotation1509404/range1509404/1
type: Range
persistent identity: range1509404
display id: range1509404
version: 1
direction: direction/forward
top level: BBa_J04450
start: 209
end: 220
orientation: inline

BBa_K1763434
type: ComponentDefinition
persistent identity: BBa K1763434
display id: BBa_K1763434
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: MaSp1-9(1C3)
creator: Vinson Lam
created: 2015-09-14T11:00:00Z
modified: 2015-09-16T11:44:18Z
discontinued: false
dominant: false
group u list: _2185_
m user id: 27136
owner id: 27136
owning group id: 9
status: status/Planning
bookmark: false
mutable description: MaSp1 9 mer in pSB1C3, no regulatory elements present.
mutable notes: no.
mutable provenance: N. clavipes
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: 8-his tag
sequence annotation: M1-AB
sequence annotation: M1-CA
sequence annotation: M1-BC
sequence: https://synbiohub.org/public/igem/BBa_K1763434_sequence/1

8-his tag
type: SequenceAnnotation
persistent identity: annotation2463973
display id: annotation2463973
version: 1
top level: BBa_K1763434
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1763434/annotation2463973/range2463973/1

https://synbiohub.org/public/igem/BBa_K1763434/annotation2463973/range2463973/1
type: Range
persistent identity: range2463973
display id: range2463973
version: 1
direction: direction/forward
top level: BBa_K1763434
start: 4
end: 27
orientation: inline

M1-AB
type: SequenceAnnotation
persistent identity: annotation2463975
display id: annotation2463975
version: 1
top level: BBa_K1763434
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763434/annotation2463975/range2463975/1

https://synbiohub.org/public/igem/BBa_K1763434/annotation2463975/range2463975/1
type: Range
persistent identity: range2463975
display id: range2463975
version: 1
direction: direction/forward
top level: BBa_K1763434
start: 648
end: 749
orientation: inline

M1-CA
type: SequenceAnnotation
persistent identity: annotation2463974
display id: annotation2463974
version: 1
top level: BBa_K1763434
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763434/annotation2463974/range2463974/1

https://synbiohub.org/public/igem/BBa_K1763434/annotation2463974/range2463974/1
type: Range
persistent identity: range2463974
display id: range2463974
version: 1
direction: direction/forward
top level: BBa_K1763434
start: 240
end: 341
orientation: inline

M1-BC
type: SequenceAnnotation
persistent identity: annotation2463980
display id: annotation2463980
version: 1
top level: BBa_K1763434
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763434/annotation2463980/range2463980/1

https://synbiohub.org/public/igem/BBa_K1763434/annotation2463980/range2463980/1
type: Range
persistent identity: range2463980
display id: range2463980
version: 1
direction: direction/forward
top level: BBa_K1763434
start: 444
end: 545
orientation: inline

M1-CA
type: SequenceAnnotation
persistent identity: annotation2463976
display id: annotation2463976
version: 1
top level: BBa_K1763434
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763434/annotation2463976/range2463976/1

https://synbiohub.org/public/igem/BBa_K1763434/annotation2463976/range2463976/1
type: Range
persistent identity: range2463976
display id: range2463976
version: 1
direction: direction/forward
top level: BBa_K1763434
start: 852
end: 953
orientation: inline

M1-AB
type: SequenceAnnotation
persistent identity: annotation2463982
display id: annotation2463982
version: 1
top level: BBa_K1763434
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763434/annotation2463982/range2463982/1

https://synbiohub.org/public/igem/BBa_K1763434/annotation2463982/range2463982/1
type: Range
persistent identity: range2463982
display id: range2463982
version: 1
direction: direction/forward
top level: BBa_K1763434
start: 36
end: 137
orientation: inline

M1-BC
type: SequenceAnnotation
persistent identity: annotation2463977
display id: annotation2463977
version: 1
top level: BBa_K1763434
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763434/annotation2463977/range2463977/1

https://synbiohub.org/public/igem/BBa_K1763434/annotation2463977/range2463977/1
type: Range
persistent identity: range2463977
display id: range2463977
version: 1
direction: direction/forward
top level: BBa_K1763434
start: 750
end: 851
orientation: inline

M1-BC
type: SequenceAnnotation
persistent identity: annotation2463978
display id: annotation2463978
version: 1
top level: BBa_K1763434
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763434/annotation2463978/range2463978/1

https://synbiohub.org/public/igem/BBa_K1763434/annotation2463978/range2463978/1
type: Range
persistent identity: range2463978
display id: range2463978
version: 1
direction: direction/forward
top level: BBa_K1763434
start: 138
end: 239
orientation: inline

M1-AB
type: SequenceAnnotation
persistent identity: annotation2463981
display id: annotation2463981
version: 1
top level: BBa_K1763434
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763434/annotation2463981/range2463981/1

https://synbiohub.org/public/igem/BBa_K1763434/annotation2463981/range2463981/1
type: Range
persistent identity: range2463981
display id: range2463981
version: 1
direction: direction/forward
top level: BBa_K1763434
start: 342
end: 443
orientation: inline

M1-CA
type: SequenceAnnotation
persistent identity: annotation2463979
display id: annotation2463979
version: 1
top level: BBa_K1763434
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763434/annotation2463979/range2463979/1

https://synbiohub.org/public/igem/BBa_K1763434/annotation2463979/range2463979/1
type: Range
persistent identity: range2463979
display id: range2463979
version: 1
direction: direction/forward
top level: BBa_K1763434
start: 546
end: 647
orientation: inline

BBa_K1582029
type: ComponentDefinition
persistent identity: BBa K1582029
display id: BBa_K1582029
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Protein Extraction Tag: Linker+sJanus
creator: Dongqi Bao
created: 2015-09-04T11:00:00Z
modified: 2015-09-09T12:05:29Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1998_
m user id: 23270
owner id: 23270
owning group id: 9
status: status/Planning
bookmark: false
mutable description: HFB1+Linker
mutable notes: HFB1+Linker
mutable provenance: HFB1+Linker
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: Linker
sequence annotation: BBa_K1582001
sequence annotation: TEV protease cleavage site
sequence: https://synbiohub.org/public/igem/BBa_K1582029_sequence/1

Linker
type: SequenceAnnotation
persistent identity: annotation2447549
display id: annotation2447549
version: 1
top level: BBa_K1582029
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1582029/annotation2447549/range2447549/1

https://synbiohub.org/public/igem/BBa_K1582029/annotation2447549/range2447549/1
type: Range
persistent identity: range2447549
display id: range2447549
version: 1
direction: direction/forward
top level: BBa_K1582029
start: 1
end: 27
orientation: inline

BBa_K1582001
type: SequenceAnnotation
persistent identity: annotation2444239
display id: annotation2444239
version: 1
top level: BBa_K1582029
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1582029/annotation2444239/range2444239/1

https://synbiohub.org/public/igem/BBa_K1582029/annotation2444239/range2444239/1
type: Range
persistent identity: range2444239
display id: range2444239
version: 1
direction: direction/forward
top level: BBa_K1582029
start: 28
end: 255
orientation: inline

TEV protease cleavage site
type: SequenceAnnotation
persistent identity: annotation2447550
display id: annotation2447550
version: 1
top level: BBa_K1582029
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1582029/annotation2447550/range2447550/1

https://synbiohub.org/public/igem/BBa_K1582029/annotation2447550/range2447550/1
type: Range
persistent identity: range2447550
display id: range2447550
version: 1
direction: direction/forward
top level: BBa_K1582029
start: 1
end: 21
orientation: inline

BBa_K1362174
type: ComponentDefinition
persistent identity: BBa K1362174
display id: BBa_K1362174
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Split sfGFP reconstitution N-terminal non-splicing control half
creator: Jan Gleixner
created: 2014-10-09T11:00:00Z
modified: 2015-05-08T01:10:05Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1738_
m user id: 0
owner id: 22830
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_J70594
component: BBa_K1362400
component: BBa_K1362471
component: BBa_K1362478
component: BBa_J18909
component: BBa_G0000
component: BBa_K1362090
sequence annotation: BBa_G0000
sequence annotation: BBa_J18909
sequence annotation: BBa_J70594
sequence annotation: BBa_K1362471
sequence annotation: BBa_K1362090
sequence annotation: BBa_K1362400
sequence annotation: BBa_K1362478
sequence: https://synbiohub.org/public/igem/BBa_K1362174_sequence/1

BBa_J70594
type: Component
persistent identity: component2414480
display id: component2414480
version: 1
top level: BBa_K1362174
access: public
definition: https://synbiohub.org/public/igem/BBa_J70594/1

BBa_K1362400
type: Component
persistent identity: component2414476
display id: component2414476
version: 1
top level: BBa_K1362174
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362400/1

BBa_K1362471
type: Component
persistent identity: component2414474
display id: component2414474
version: 1
top level: BBa_K1362174
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362471/1

BBa_K1362478
type: Component
persistent identity: component2414475
display id: component2414475
version: 1
top level: BBa_K1362174
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362478/1

BBa_J18909
type: Component
persistent identity: component2414478
display id: component2414478
version: 1
top level: BBa_K1362174
access: public
definition: https://synbiohub.org/public/igem/BBa_J18909/1

BBa_G0000
type: Component
persistent identity: component2414470
display id: component2414470
version: 1
top level: BBa_K1362174
access: public
definition: https://synbiohub.org/public/igem/BBa_G0000/1

BBa_K1362090
type: Component
persistent identity: component2414469
display id: component2414469
version: 1
top level: BBa_K1362174
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362090/1

BBa_G0000
type: SequenceAnnotation
persistent identity: annotation2414470
display id: annotation2414470
version: 1
top level: BBa_K1362174
location: https://synbiohub.org/public/igem/BBa_K1362174/annotation2414470/range2414470/1
component: BBa_G0000

https://synbiohub.org/public/igem/BBa_K1362174/annotation2414470/range2414470/1
type: Range
persistent identity: range2414470
display id: range2414470
version: 1
direction: direction/reverse
top level: BBa_K1362174
start: 30
end: 35
orientation: inline

BBa_J18909
type: SequenceAnnotation
persistent identity: annotation2414478
display id: annotation2414478
version: 1
top level: BBa_K1362174
location: https://synbiohub.org/public/igem/BBa_K1362174/annotation2414478/range2414478/1
component: BBa_J18909

https://synbiohub.org/public/igem/BBa_K1362174/annotation2414478/range2414478/1
type: Range
persistent identity: range2414478
display id: range2414478
version: 1
direction: direction/reverse
top level: BBa_K1362174
start: 552
end: 569
orientation: inline

BBa_J70594
type: SequenceAnnotation
persistent identity: annotation2414480
display id: annotation2414480
version: 1
top level: BBa_K1362174
location: https://synbiohub.org/public/igem/BBa_K1362174/annotation2414480/range2414480/1
component: BBa_J70594

https://synbiohub.org/public/igem/BBa_K1362174/annotation2414480/range2414480/1
type: Range
persistent identity: range2414480
display id: range2414480
version: 1
direction: direction/reverse
top level: BBa_K1362174
start: 570
end: 575
orientation: inline

BBa_K1362471
type: SequenceAnnotation
persistent identity: annotation2414474
display id: annotation2414474
version: 1
top level: BBa_K1362174
location: https://synbiohub.org/public/igem/BBa_K1362174/annotation2414474/range2414474/1
component: BBa_K1362471

https://synbiohub.org/public/igem/BBa_K1362174/annotation2414474/range2414474/1
type: Range
persistent identity: range2414474
display id: range2414474
version: 1
direction: direction/reverse
top level: BBa_K1362174
start: 36
end: 245
orientation: inline

BBa_K1362090
type: SequenceAnnotation
persistent identity: annotation2414469
display id: annotation2414469
version: 1
top level: BBa_K1362174
location: https://synbiohub.org/public/igem/BBa_K1362174/annotation2414469/range2414469/1
component: BBa_K1362090

https://synbiohub.org/public/igem/BBa_K1362174/annotation2414469/range2414469/1
type: Range
persistent identity: range2414469
display id: range2414469
version: 1
direction: direction/reverse
top level: BBa_K1362174
start: 1
end: 29
orientation: inline

BBa_K1362400
type: SequenceAnnotation
persistent identity: annotation2414476
display id: annotation2414476
version: 1
top level: BBa_K1362174
location: https://synbiohub.org/public/igem/BBa_K1362174/annotation2414476/range2414476/1
component: BBa_K1362400

https://synbiohub.org/public/igem/BBa_K1362174/annotation2414476/range2414476/1
type: Range
persistent identity: range2414476
display id: range2414476
version: 1
direction: direction/reverse
top level: BBa_K1362174
start: 250
end: 551
orientation: inline

BBa_K1362478
type: SequenceAnnotation
persistent identity: annotation2414475
display id: annotation2414475
version: 1
top level: BBa_K1362174
location: https://synbiohub.org/public/igem/BBa_K1362174/annotation2414475/range2414475/1
component: BBa_K1362478

https://synbiohub.org/public/igem/BBa_K1362174/annotation2414475/range2414475/1
type: Range
persistent identity: range2414475
display id: range2414475
version: 1
direction: direction/reverse
top level: BBa_K1362174
start: 246
end: 249
orientation: inline

BBa_K1758325
type: ComponentDefinition
persistent identity: BBa K1758325
display id: BBa_K1758325
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Copper responsive promoter with T7-promoter and UTR-sfGFP
creator: Team Bielefeld-CeBiTec 2015
created: 2015-09-07T11:00:00Z
modified: 2016-01-06T03:42:09Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2180_
m user id: 4206
owner id: 26630
owning group id: 9
status: status/Planning
bookmark: false
mutable description: ...
mutable notes: ...
mutable provenance: ...
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1758323
component: BBa_I719005
sequence annotation: BBa_K1758323
sequence annotation: BBa_I719005
sequence: https://synbiohub.org/public/igem/BBa_K1758325_sequence/1

BBa_K1758323
type: Component
persistent identity: component2447488
display id: component2447488
version: 1
top level: BBa_K1758325
access: public
definition: BBa_K1758323

BBa_I719005
type: Component
persistent identity: component2447482
display id: component2447482
version: 1
top level: BBa_K1758325
access: public
definition: https://synbiohub.org/public/igem/BBa_I719005/1

BBa_K1758323
type: SequenceAnnotation
persistent identity: annotation2447488
display id: annotation2447488
version: 1
top level: BBa_K1758325
location: https://synbiohub.org/public/igem/BBa_K1758325/annotation2447488/range2447488/1
component: BBa_K1758323

https://synbiohub.org/public/igem/BBa_K1758325/annotation2447488/range2447488/1
type: Range
persistent identity: range2447488
display id: range2447488
version: 1
direction: direction/reverse
top level: BBa_K1758325
start: 32
end: 1023
orientation: inline

BBa_I719005
type: SequenceAnnotation
persistent identity: annotation2447482
display id: annotation2447482
version: 1
top level: BBa_K1758325
location: https://synbiohub.org/public/igem/BBa_K1758325/annotation2447482/range2447482/1
component: BBa_I719005

https://synbiohub.org/public/igem/BBa_K1758325/annotation2447482/range2447482/1
type: Range
persistent identity: range2447482
display id: range2447482
version: 1
direction: direction/reverse
top level: BBa_K1758325
start: 1
end: 23
orientation: inline

BBa_K1758360
type: ComponentDefinition
persistent identity: BBa K1758360
display id: BBa_K1758360
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: gabR gene and gabT promoter from Bacillus subtilis
creator: Team Bielefeld-CeBiTec 2015
created: 2015-08-21T11:00:00Z
modified: 2015-09-18T04:39:39Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2180_
m user id: 27935
owner id: 26587
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This is a part of the gab operon of Bacillus subtilis. It includes the gene for the repressor protein GabR under the control of its natural promoter as well as the gabT promoter, which is regulated by GabR. GabR is inactivated by γ-aminobutyrate(GABA). The expression of any gene that is inserted downstream of this part can therefore be induced by GABA.
mutable notes: The coding sequence of gabR is codon optimized for expression in E. coli.
mutable provenance: This part was obtained by gene synthesis.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: gabR
sequence: https://synbiohub.org/public/igem/BBa_K1758360_sequence/1

gabR
type: SequenceAnnotation
persistent identity: annotation2436848
display id: annotation2436848
version: 1
top level: BBa_K1758360
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1758360/annotation2436848/range2436848/1

https://synbiohub.org/public/igem/BBa_K1758360/annotation2436848/range2436848/1
type: Range
persistent identity: range2436848
display id: range2436848
version: 1
direction: direction/reverse
top level: BBa_K1758360
start: 1
end: 1440
orientation: inline

BBa_K1763009
type: ComponentDefinition
persistent identity: BBa K1763009
display id: BBa_K1763009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Major Spidroin Protein 2 (MaSp2) Sequencing Core with AB Sticky Ends
creator: Vinson Lam
created: 2015-07-26T11:00:00Z
modified: 2015-09-16T10:43:11Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2185_
m user id: 27136
owner id: 27136
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This part contains the core sequence of MaSp2 that has been assembled with a 5'-AGTT-3' overhang on the 5' end of the sequence and 3'-ACAG-5' overhang on the 3' end of the sequence. This part was designed for use with ICA, along with parts BBa_K1763003 and BBa_K1763004 as an efficient method to assemble multiple MaSp2 sequences together. This setup is to show that this particular set of sticky ends is suitable for ICA. Before using in ICA, this part should be digested with BsaI, a type IIs restriction enzyme, which cuts DNA outside of its recognition site. This part codes for identical protein primary sequence as BBa_K17630002, but has a different DNA sequence. This part is intended to anneal to a sequencing primer that will allow longer composite MaSp genes to be read.
mutable notes: The gene sequence for MaSp2 is particularly GC-rich, which may complicate PCR.
mutable provenance: This part is derived from the MaSp2 gene found in the spider N. Clavipes.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: BsaI recognition site(reverse)
sequence annotation: SeqF Primer Binding Site
sequence annotation: A sticky end
sequence annotation: SeqR Primer Binding Site
sequence annotation: B sticky end
sequence annotation: BsaI recognition site (forward)
sequence annotation: MaSp2 Core Sequence
sequence: https://synbiohub.org/public/igem/BBa_K1763009_sequence/1

BsaI recognition site(reverse)
type: SequenceAnnotation
persistent identity: annotation2454683
display id: annotation2454683
version: 1
top level: BBa_K1763009
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1763009/annotation2454683/range2454683/1

https://synbiohub.org/public/igem/BBa_K1763009/annotation2454683/range2454683/1
type: Range
persistent identity: range2454683
display id: range2454683
version: 1
direction: direction/reverse
top level: BBa_K1763009
start: 114
end: 119
orientation: inline

SeqF Primer Binding Site
type: SequenceAnnotation
persistent identity: annotation2473347
display id: annotation2473347
version: 1
top level: BBa_K1763009
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K1763009/annotation2473347/range2473347/1

https://synbiohub.org/public/igem/BBa_K1763009/annotation2473347/range2473347/1
type: Range
persistent identity: range2473347
display id: range2473347
version: 1
direction: direction/forward
top level: BBa_K1763009
start: 47
end: 64
orientation: inline

A sticky end
type: SequenceAnnotation
persistent identity: annotation2463770
display id: annotation2463770
version: 1
top level: BBa_K1763009
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1763009/annotation2463770/range2463770/1

https://synbiohub.org/public/igem/BBa_K1763009/annotation2463770/range2463770/1
type: Range
persistent identity: range2463770
display id: range2463770
version: 1
direction: direction/forward
top level: BBa_K1763009
start: 7
end: 10
orientation: inline

SeqR Primer Binding Site
type: SequenceAnnotation
persistent identity: annotation2473348
display id: annotation2473348
version: 1
top level: BBa_K1763009
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K1763009/annotation2473348/range2473348/1

https://synbiohub.org/public/igem/BBa_K1763009/annotation2473348/range2473348/1
type: Range
persistent identity: range2473348
display id: range2473348
version: 1
direction: direction/reverse
top level: BBa_K1763009
start: 47
end: 64
orientation: inline

B sticky end
type: SequenceAnnotation
persistent identity: annotation2463771
display id: annotation2463771
version: 1
top level: BBa_K1763009
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1763009/annotation2463771/range2463771/1

https://synbiohub.org/public/igem/BBa_K1763009/annotation2463771/range2463771/1
type: Range
persistent identity: range2463771
display id: range2463771
version: 1
direction: direction/forward
top level: BBa_K1763009
start: 109
end: 112
orientation: inline

BsaI recognition site (forward)
type: SequenceAnnotation
persistent identity: annotation2433551
display id: annotation2433551
version: 1
top level: BBa_K1763009
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1763009/annotation2433551/range2433551/1

https://synbiohub.org/public/igem/BBa_K1763009/annotation2433551/range2433551/1
type: Range
persistent identity: range2433551
display id: range2433551
version: 1
direction: direction/forward
top level: BBa_K1763009
start: 1
end: 5
orientation: inline

MaSp2 Core Sequence
type: SequenceAnnotation
persistent identity: annotation2473130
display id: annotation2473130
version: 1
top level: BBa_K1763009
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763009/annotation2473130/range2473130/1

https://synbiohub.org/public/igem/BBa_K1763009/annotation2473130/range2473130/1
type: Range
persistent identity: range2473130
display id: range2473130
version: 1
direction: direction/forward
top level: BBa_K1763009
start: 10
end: 108
orientation: inline

BBa_K1763011
type: ComponentDefinition
persistent identity: BBa K1763011
display id: BBa_K1763011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Major Spidroin Protein 1 (MaSp1) with Sticky Ends BC
creator: Vinson Lam
created: 2015-07-26T11:00:00Z
modified: 2015-09-16T10:44:29Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2185_
m user id: 27136
owner id: 27136
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This part contains the core sequence of MaSp1 that has been assembled with a 5'-TGTC-3' overhang on the 5' end of the sequence and 3'-GCAC-5' overhang on the 3' end of the sequence. This part was designed for use with ICA, along with parts BBa_K1763003 and BBa_K1763004 as an efficient method to assemble multiple MaSp sequences together. This setup is to show that this particular set of sticky ends is suitable for ICA. Before using in ICA, this part should be digested with BsaI, a type IIs restriction enzyme, which cuts DNA outside of its recognition site.
mutable notes: The gene sequence for MaSp2 is particularly GC-rich, which may complicate PCR.
mutable provenance: This part is derived from the MaSp2 gene found in the spider N. Clavipes.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: BsaI recognition site (forward)
sequence annotation: BsaI site recognition site (reverse)
sequence annotation: MaSp1 Core
sequence annotation: C sticky end
sequence annotation: B sticky end
sequence: https://synbiohub.org/public/igem/BBa_K1763011_sequence/1

BsaI recognition site (forward)
type: SequenceAnnotation
persistent identity: annotation2433554
display id: annotation2433554
version: 1
top level: BBa_K1763011
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1763011/annotation2433554/range2433554/1

https://synbiohub.org/public/igem/BBa_K1763011/annotation2433554/range2433554/1
type: Range
persistent identity: range2433554
display id: range2433554
version: 1
direction: direction/forward
top level: BBa_K1763011
start: 1
end: 5
orientation: inline

BsaI site recognition site (reverse)
type: SequenceAnnotation
persistent identity: annotation2433555
display id: annotation2433555
version: 1
top level: BBa_K1763011
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1763011/annotation2433555/range2433555/1

https://synbiohub.org/public/igem/BBa_K1763011/annotation2433555/range2433555/1
type: Range
persistent identity: range2433555
display id: range2433555
version: 1
direction: direction/reverse
top level: BBa_K1763011
start: 114
end: 119
orientation: inline

MaSp1 Core
type: SequenceAnnotation
persistent identity: annotation2473360
display id: annotation2473360
version: 1
top level: BBa_K1763011
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763011/annotation2473360/range2473360/1

https://synbiohub.org/public/igem/BBa_K1763011/annotation2473360/range2473360/1
type: Range
persistent identity: range2473360
display id: range2473360
version: 1
direction: direction/forward
top level: BBa_K1763011
start: 11
end: 108
orientation: inline

C sticky end
type: SequenceAnnotation
persistent identity: annotation2463775
display id: annotation2463775
version: 1
top level: BBa_K1763011
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1763011/annotation2463775/range2463775/1

https://synbiohub.org/public/igem/BBa_K1763011/annotation2463775/range2463775/1
type: Range
persistent identity: range2463775
display id: range2463775
version: 1
direction: direction/forward
top level: BBa_K1763011
start: 109
end: 112
orientation: inline

B sticky end
type: SequenceAnnotation
persistent identity: annotation2463774
display id: annotation2463774
version: 1
top level: BBa_K1763011
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1763011/annotation2463774/range2463774/1

https://synbiohub.org/public/igem/BBa_K1763011/annotation2463774/range2463774/1
type: Range
persistent identity: range2463774
display id: range2463774
version: 1
direction: direction/forward
top level: BBa_K1763011
start: 7
end: 10
orientation: inline

RBS-xylC
type: ComponentDefinition
persistent identity: BBa K1602001
display id: BBa_K1602001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0034-xylC
creator: Jonas Sindlinger, Leon Kraus, Jonathan Pletzer-Zelgert, Elena Tonchevska, Sebastian Holzner, Mirjam Haun, Sebastian Barthel
created: 2015-08-27T11:00:00Z
modified: 2015-09-18T12:11:36Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2019_
m user id: 22022
owner id: 25989
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Blank
mutable notes: Blank
mutable provenance: E.coli TOP10
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_B0034
component: BBa_K1602010
sequence annotation: BBa_K1602010
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K1602001_sequence/1

BBa_B0034
type: Component
persistent identity: component2463052
display id: component2463052
version: 1
top level: RBS-xylC
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1602010
type: Component
persistent identity: component2463053
display id: component2463053
version: 1
top level: RBS-xylC
access: public
definition: BBa_K1602010

BBa_K1602010
type: SequenceAnnotation
persistent identity: annotation2463053
display id: annotation2463053
version: 1
top level: RBS-xylC
location: https://synbiohub.org/public/igem/BBa_K1602001/annotation2463053/range2463053/1
component: BBa_K1602010

https://synbiohub.org/public/igem/BBa_K1602001/annotation2463053/range2463053/1
type: Range
persistent identity: range2463053
display id: range2463053
version: 1
direction: direction/reverse
top level: RBS-xylC
start: 19
end: 888
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2463052
display id: annotation2463052
version: 1
top level: RBS-xylC
location: https://synbiohub.org/public/igem/BBa_K1602001/annotation2463052/range2463052/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1602001/annotation2463052/range2463052/1
type: Range
persistent identity: range2463052
display id: range2463052
version: 1
direction: direction/reverse
top level: RBS-xylC
start: 1
end: 12
orientation: inline

BBa_K1497031
type: ComponentDefinition
persistent identity: BBa K1497031
display id: BBa_K1497031
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Scaffold (G-S-P-His)
creator: Sascha Hein, Rene Sahm, Malte Blumenroth, Tobias Gabriel, Sebastian Palluk
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:46Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1877_
m user id: 0
owner id: 12466
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Scaffold (G-S-P-His)
mutable notes: Scaffold (G-S-P-His)
mutable provenance: Scaffold (G-S-P-His)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1497031_sequence/1

BBa_K1604021
type: ComponentDefinition
persistent identity: BBa K1604021
display id: BBa_K1604021
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23100 + β-carotene 15,15’-dioxygenase (blh gene)
creator: Veronica Pinamonti
created: 2015-08-17T11:00:00Z
modified: 2015-09-18T03:56:40Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2021_
m user id: 24580
owner id: 24581
owning group id: 9
status: status/Planning
bookmark: false
mutable description: blh encodes for β-carotene 15,15'-dioxygenase, the enzyme that cleaves one molecule of β-carotene into two molecules of retinal
mutable notes: The part was subcloned from pUC57 into pSB1C3
mutable provenance: The sequence of uncultered cluster bacteria SAR86 was synthetized and codon optimized by GeneScript
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J23100
component: BBa_K1604019
sequence annotation: BBa_K1604019
sequence annotation: BBa_J23100
sequence: https://synbiohub.org/public/igem/BBa_K1604021_sequence/1

BBa_J23100
type: Component
persistent identity: component2449238
display id: component2449238
version: 1
top level: BBa_K1604021
access: public
definition: https://synbiohub.org/public/igem/BBa_J23100/1

BBa_K1604019
type: Component
persistent identity: component2449242
display id: component2449242
version: 1
top level: BBa_K1604021
access: public
definition: https://synbiohub.org/public/igem/BBa_K1604019/1

BBa_K1604019
type: SequenceAnnotation
persistent identity: annotation2449242
display id: annotation2449242
version: 1
top level: BBa_K1604021
location: https://synbiohub.org/public/igem/BBa_K1604021/annotation2449242/range2449242/1
component: BBa_K1604019

https://synbiohub.org/public/igem/BBa_K1604021/annotation2449242/range2449242/1
type: Range
persistent identity: range2449242
display id: range2449242
version: 1
direction: direction/reverse
top level: BBa_K1604021
start: 44
end: 887
orientation: inline

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2449238
display id: annotation2449238
version: 1
top level: BBa_K1604021
location: https://synbiohub.org/public/igem/BBa_K1604021/annotation2449238/range2449238/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K1604021/annotation2449238/range2449238/1
type: Range
persistent identity: range2449238
display id: range2449238
version: 1
direction: direction/reverse
top level: BBa_K1604021
start: 1
end: 35
orientation: inline

BBa_K1315006
type: ComponentDefinition
persistent identity: BBa K1315006
display id: BBa_K1315006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: The manA promoter from Xanthomonas campestris
creator: Dimitrios Michailidis
created: 2014-09-25T11:00:00Z
modified: 2015-05-08T01:09:50Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1690_
m user id: 0
owner id: 21812
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is the promoter manA from Xcc.
mutable notes: The sequence did not have any restriction sites to remove so it was immediately PCRed out of the genome and had the registry prefix and suffix added to it. It was then ligated into the pSB1C3 vector.
mutable provenance: The source of this part is Xanthomonas campestris pv. campestris 8004 genomic DNA which was provided by the Robert Ryan lab in the Molecular Microbiology department of the University of Dundee, UK.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: manA
sequence: https://synbiohub.org/public/igem/BBa_K1315006_sequence/1

manA
type: SequenceAnnotation
persistent identity: annotation2386904
display id: annotation2386904
version: 1
top level: BBa_K1315006
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1315006/annotation2386904/range2386904/1

https://synbiohub.org/public/igem/BBa_K1315006/annotation2386904/range2386904/1
type: Range
persistent identity: range2386904
display id: range2386904
version: 1
direction: direction/forward
top level: BBa_K1315006
start: 1
end: 250
orientation: inline

BBa_K1325002
type: ComponentDefinition
persistent identity: BBa K1325002
display id: BBa_K1325002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: sicA*??????Chaperone
creator: Ke Xu
created: 2014-09-27T11:00:00Z
modified: 2015-05-08T01:09:54Z
discontinued: false
dominant: false
group u list: _1700_
m user id: 0
owner id: 23253
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Chaperone,the SicA???InvF complex activates transcription from the psicA promoter
mutable notes: no
mutable provenance: type III secretion pathways
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1325002_sequence/1

BBa_K1758323
type: ComponentDefinition
persistent identity: BBa K1758323
display id: BBa_K1758323
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: UTR-sfGFP under control of Copper responsive promoter
creator: Team Bielefeld-CeBiTec 2015
created: 2015-08-28T11:00:00Z
modified: 2016-01-06T03:42:20Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2180_
m user id: 4206
owner id: 26630
owning group id: 9
status: status/Planning
bookmark: false
mutable description: copper responsive promoter with 5?? untranslated region and sfGFP for detection
mutable notes: ...
mutable provenance: copper responsive promoter of ????E.coli`` with sfGFP for detection
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: sfGFP
sequence annotation: T7-term
sequence annotation: 5??UTR
sequence annotation: CopAp
sequence annotation: T1
sequence: https://synbiohub.org/public/igem/BBa_K1758323_sequence/1

sfGFP
type: SequenceAnnotation
persistent identity: annotation2439963
display id: annotation2439963
version: 1
top level: BBa_K1758323
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1758323/annotation2439963/range2439963/1

https://synbiohub.org/public/igem/BBa_K1758323/annotation2439963/range2439963/1
type: Range
persistent identity: range2439963
display id: range2439963
version: 1
direction: direction/forward
top level: BBa_K1758323
start: 136
end: 855
orientation: inline

T7-term
type: SequenceAnnotation
persistent identity: annotation2439965
display id: annotation2439965
version: 1
top level: BBa_K1758323
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1758323/annotation2439965/range2439965/1

https://synbiohub.org/public/igem/BBa_K1758323/annotation2439965/range2439965/1
type: Range
persistent identity: range2439965
display id: range2439965
version: 1
direction: direction/forward
top level: BBa_K1758323
start: 952
end: 992
orientation: inline

5??UTR
type: SequenceAnnotation
persistent identity: annotation2439962
display id: annotation2439962
version: 1
top level: BBa_K1758323
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1758323/annotation2439962/range2439962/1

https://synbiohub.org/public/igem/BBa_K1758323/annotation2439962/range2439962/1
type: Range
persistent identity: range2439962
display id: range2439962
version: 1
direction: direction/forward
top level: BBa_K1758323
start: 90
end: 135
orientation: inline

CopAp
type: SequenceAnnotation
persistent identity: annotation2439961
display id: annotation2439961
version: 1
top level: BBa_K1758323
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1758323/annotation2439961/range2439961/1

https://synbiohub.org/public/igem/BBa_K1758323/annotation2439961/range2439961/1
type: Range
persistent identity: range2439961
display id: range2439961
version: 1
direction: direction/forward
top level: BBa_K1758323
start: 1
end: 83
orientation: inline

T1
type: SequenceAnnotation
persistent identity: annotation2439964
display id: annotation2439964
version: 1
top level: BBa_K1758323
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1758323/annotation2439964/range2439964/1

https://synbiohub.org/public/igem/BBa_K1758323/annotation2439964/range2439964/1
type: Range
persistent identity: range2439964
display id: range2439964
version: 1
direction: direction/forward
top level: BBa_K1758323
start: 864
end: 943
orientation: inline

BBa_K1321352
type: ComponentDefinition
persistent identity: BBa K1321352
display id: BBa_K1321352
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NiBP fused to CBDcex driven by T7
creator: Chris N Micklem
created: 2014-10-07T11:00:00Z
modified: 2015-06-17T12:31:54Z
discontinued: false
dominant: false
group u list: _1696_
m user id: 4206
owner id: 20830
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: asdf
mutable notes: asdf
mutable provenance: asdf
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1321344
component: BBa_K1321338
component: BBa_K1319106
sequence annotation: BBa_K1321344
sequence annotation: BBa_K1319106
sequence annotation: BBa_K1321338
sequence: https://synbiohub.org/public/igem/BBa_K1321352_sequence/1

BBa_K1321344
type: Component
persistent identity: component2415435
display id: component2415435
version: 1
top level: BBa_K1321352
access: public
definition: BBa_K1321344

BBa_K1321338
type: Component
persistent identity: component2415428
display id: component2415428
version: 1
top level: BBa_K1321352
access: public
definition: BBa_K1321338

BBa_K1319106
type: Component
persistent identity: component2415430
display id: component2415430
version: 1
top level: BBa_K1321352
access: public
definition: https://synbiohub.org/public/igem/BBa_K1319106/1

BBa_K1321344
type: SequenceAnnotation
persistent identity: annotation2415435
display id: annotation2415435
version: 1
top level: BBa_K1321352
location: https://synbiohub.org/public/igem/BBa_K1321352/annotation2415435/range2415435/1
component: BBa_K1321344

https://synbiohub.org/public/igem/BBa_K1321352/annotation2415435/range2415435/1
type: Range
persistent identity: range2415435
display id: range2415435
version: 1
direction: direction/reverse
top level: BBa_K1321352
start: 59
end: 567
orientation: inline

BBa_K1319106
type: SequenceAnnotation
persistent identity: annotation2415430
display id: annotation2415430
version: 1
top level: BBa_K1321352
location: https://synbiohub.org/public/igem/BBa_K1321352/annotation2415430/range2415430/1
component: BBa_K1319106

https://synbiohub.org/public/igem/BBa_K1321352/annotation2415430/range2415430/1
type: Range
persistent identity: range2415430
display id: range2415430
version: 1
direction: direction/reverse
top level: BBa_K1321352
start: 44
end: 58
orientation: inline

BBa_K1321338
type: SequenceAnnotation
persistent identity: annotation2415428
display id: annotation2415428
version: 1
top level: BBa_K1321352
location: https://synbiohub.org/public/igem/BBa_K1321352/annotation2415428/range2415428/1
component: BBa_K1321338

https://synbiohub.org/public/igem/BBa_K1321352/annotation2415428/range2415428/1
type: Range
persistent identity: range2415428
display id: range2415428
version: 1
direction: direction/reverse
top level: BBa_K1321352
start: 1
end: 43
orientation: inline

BBa_K1806003
type: ComponentDefinition
persistent identity: BBa K1806003
display id: BBa_K1806003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7+ ibPB RNA Thermometer + lacI
creator: İBRAHİM YASİR ORHAN
created: 2015-09-16T11:00:00Z
modified: 2015-09-17T07:15:35Z
discontinued: false
dominant: false
group u list: _973_2231_
m user id: 11153
owner id: 11153
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Designed to close lacO present systems in higher temperatures by producing lacI.
mutable notes: none
mutable provenance: The part is a composite part formed up of several distinct parts.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence: https://synbiohub.org/public/igem/BBa_K1806003_sequence/1

BBa_K1499500
type: ComponentDefinition
persistent identity: BBa K1499500
display id: BBa_K1499500
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: quorum sensing machinery that activates GFP expression
creator: Poorwa Godbole
created: 2014-08-24T11:00:00Z
modified: 2015-05-08T01:10:46Z
discontinued: false
dominant: false
group u list: _1879_
m user id: 0
owner id: 22138
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part is the combination of two parts, BBa_I13202 and BBa_T9002. The first part (BBa_I13202) produces a luxI enzyme under the control of a lac repressible promoter. The second part (BBa_T9002) produces luxR, which combines with the luxI to activate the promoter that controls expression of GFP.
mutable notes: N/A
mutable provenance: Combination of two previous parts.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_T9002
component: BBa_I13202
sequence annotation: BBa_I13202
sequence annotation: BBa_T9002
sequence: https://synbiohub.org/public/igem/BBa_K1499500_sequence/1

BBa_T9002
type: Component
persistent identity: component2381403
display id: component2381403
version: 1
top level: BBa_K1499500
access: public
definition: https://synbiohub.org/public/igem/BBa_T9002/1

BBa_I13202
type: Component
persistent identity: component2381368
display id: component2381368
version: 1
top level: BBa_K1499500
access: public
definition: https://synbiohub.org/public/igem/BBa_I13202/1

BBa_I13202
type: SequenceAnnotation
persistent identity: annotation2381368
display id: annotation2381368
version: 1
top level: BBa_K1499500
location: https://synbiohub.org/public/igem/BBa_K1499500/annotation2381368/range2381368/1
component: BBa_I13202

https://synbiohub.org/public/igem/BBa_K1499500/annotation2381368/range2381368/1
type: Range
persistent identity: range2381368
display id: range2381368
version: 1
direction: direction/reverse
top level: BBa_K1499500
start: 1
end: 803
orientation: inline

BBa_T9002
type: SequenceAnnotation
persistent identity: annotation2381403
display id: annotation2381403
version: 1
top level: BBa_K1499500
location: https://synbiohub.org/public/igem/BBa_K1499500/annotation2381403/range2381403/1
component: BBa_T9002

https://synbiohub.org/public/igem/BBa_K1499500/annotation2381403/range2381403/1
type: Range
persistent identity: range2381403
display id: range2381403
version: 1
direction: direction/reverse
top level: BBa_K1499500
start: 812
end: 2756
orientation: inline

tEno2
type: ComponentDefinition
persistent identity: BBa K1829003
display id: BBa_K1829003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: tEno2: Yeast terminator
creator: Nicholas Suen
created: 2015-09-09T11:00:00Z
modified: 2015-09-17T02:28:03Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2255_
m user id: 27842
owner id: 27842
owning group id: 9
status: status/Planning
bookmark: false
mutable description: tENO2 is a terminator
mutable notes: cats
mutable provenance: cats
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence: https://synbiohub.org/public/igem/BBa_K1829003_sequence/1

BBa_K1321120
type: ComponentDefinition
persistent identity: BBa K1321120
display id: BBa_K1321120
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SmtA metallothionein fused to linker-dCBD
creator: Gabriella Santosa
created: 2014-10-08T11:00:00Z
modified: 2015-06-17T12:22:14Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 4206
owner id: 20961
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: GATC
mutable notes: GATC
mutable provenance: GATC
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_B0105
component: BBa_K1321340
component: BBa_K1321011
sequence annotation: BBa_B0105
sequence annotation: BBa_K1321340
sequence annotation: BBa_K1321011
sequence: https://synbiohub.org/public/igem/BBa_K1321120_sequence/1

BBa_B0105
type: Component
persistent identity: component2414677
display id: component2414677
version: 1
top level: BBa_K1321120
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_K1321340
type: Component
persistent identity: component2414679
display id: component2414679
version: 1
top level: BBa_K1321120
access: public
definition: BBa_K1321340

BBa_K1321011
type: Component
persistent identity: component2414675
display id: component2414675
version: 1
top level: BBa_K1321120
access: public
definition: https://synbiohub.org/public/igem/BBa_K1321011/1

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2414677
display id: annotation2414677
version: 1
top level: BBa_K1321120
location: https://synbiohub.org/public/igem/BBa_K1321120/annotation2414677/range2414677/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_K1321120/annotation2414677/range2414677/1
type: Range
persistent identity: range2414677
display id: range2414677
version: 1
direction: direction/reverse
top level: BBa_K1321120
start: 166
end: 171
orientation: inline

BBa_K1321340
type: SequenceAnnotation
persistent identity: annotation2414679
display id: annotation2414679
version: 1
top level: BBa_K1321120
location: https://synbiohub.org/public/igem/BBa_K1321120/annotation2414679/range2414679/1
component: BBa_K1321340

https://synbiohub.org/public/igem/BBa_K1321120/annotation2414679/range2414679/1
type: Range
persistent identity: range2414679
display id: range2414679
version: 1
direction: direction/reverse
top level: BBa_K1321120
start: 172
end: 537
orientation: inline

BBa_K1321011
type: SequenceAnnotation
persistent identity: annotation2414675
display id: annotation2414675
version: 1
top level: BBa_K1321120
location: https://synbiohub.org/public/igem/BBa_K1321120/annotation2414675/range2414675/1
component: BBa_K1321011

https://synbiohub.org/public/igem/BBa_K1321120/annotation2414675/range2414675/1
type: Range
persistent identity: range2414675
display id: range2414675
version: 1
direction: direction/reverse
top level: BBa_K1321120
start: 1
end: 165
orientation: inline

GAFP-1
type: ComponentDefinition
persistent identity: BBa K1364007
display id: BBa_K1364007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS + Antifungal GAFP-1 + Double terminator
creator: Jourdan Camille
created: 2014-10-02T11:00:00Z
modified: 2015-06-08T08:40:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1740_
m user id: 4206
owner id: 21465
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: bla bla
mutable notes: bla
mutable provenance: bla
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0015
component: BBa_K1364002
sequence annotation: BBa_B0015
sequence annotation: BBa_K1364002
sequence: https://synbiohub.org/public/igem/BBa_K1364007_sequence/1

BBa_B0015
type: Component
persistent identity: component2392854
display id: component2392854
version: 1
top level: GAFP-1
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_K1364002
type: Component
persistent identity: component2392847
display id: component2392847
version: 1
top level: GAFP-1
access: public
definition: https://synbiohub.org/public/igem/BBa_K1364002/1

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2392854
display id: annotation2392854
version: 1
top level: GAFP-1
location: https://synbiohub.org/public/igem/BBa_K1364007/annotation2392854/range2392854/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1364007/annotation2392854/range2392854/1
type: Range
persistent identity: range2392854
display id: range2392854
version: 1
direction: direction/reverse
top level: GAFP-1
start: 196
end: 324
orientation: inline

BBa_K1364002
type: SequenceAnnotation
persistent identity: annotation2392847
display id: annotation2392847
version: 1
top level: GAFP-1
location: https://synbiohub.org/public/igem/BBa_K1364007/annotation2392847/range2392847/1
component: BBa_K1364002

https://synbiohub.org/public/igem/BBa_K1364007/annotation2392847/range2392847/1
type: Range
persistent identity: range2392847
display id: range2392847
version: 1
direction: direction/reverse
top level: GAFP-1
start: 1
end: 187
orientation: inline

BBa_K1351001
type: ComponentDefinition
persistent identity: BBa K1351001
display id: BBa_K1351001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Contactin-4-derived peptide binding to NADH Oxidase of S. pneumoniae
creator: Mona Dotzler
created: 2014-10-02T11:00:00Z
modified: 2015-06-01T12:51:25Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1726_
m user id: 4206
owner id: 20048
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Short peptide of 13 amino acids corresponding to amino acids of the human protein Contactin-4 (isoform 3, UniProt Q8IWV2-3). It has been shown to bind to NADH oxidase (NOX) of Streptococcus pneumoniae and inhibit bacterial binding to human A549 cells (Muchnik et. al., 2013). Displayed on the surface of Bacillus subtilis, it should enable B. subtilis to bind specifically to S. pneumoniae. This part was generated in a modified version of RFC25, where a strong Shine Dalgarno Sequence (SD) is included, and has the following prefix and suffix: {| |prefix with EcoRI, NotI, XbaI, SD and NgoMIV: |GAATTCGCGGCCGCTTCTAGAGTAAGGAGGAGCCGGC |- |suffix with AgeI, SpeI, NotI and PstI: |ACCGGTTAATACTAGTAGCGGCCGCCTGCAG |} Sites of restriction enzymes generating compatible overhangs have the same color: EcoRI and PstI in blue, NotI in green, XbaI and SpeI in red, NgoMIV and AgeI in orange. Shine-Dalgarno sequence and stop codons are underlined.
mutable notes: For generation of the part's nucleotide sequence, the amino acid sequence of the peptide (Muchnik et. al., 2013) was reverse transcribed with regard to the codon usage of Bacillus subtilis. Oligos were designed as precut by EcoRI and PstI to shorten the synthesized fragments and to simplify the cloning into pSB1C3.
mutable provenance: The part was generated by annealing of artificially synthesized oligonucletides: C4P_ENX_SD_Ngo_precutEP_fwd: AATTCgcggccgctTCTAGAgtaaggaggaaGCCGGCATG TGGGAATGTAAAGCGAACGGAAGACCGAAACCGACATAT ACCGGTtaatACTAGTagcggccgCTGCA C4P_SNP_Age_precutEP_rev: GcggccgctACTAGTattaACCGGT ATATGTCGGTTTCGGTCTTCCGTTCGCTTTACATTCCCACATGCCGGCttcctccttacTCTAGAagcggccgcG
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1351001_sequence/1

DspBx
type: ComponentDefinition
persistent identity: BBa K1659210
display id: BBa_K1659210
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Dispersin B with mutation at nucleotide 582 (T to C)
creator: Wei Chung Kong
created: 2015-08-27T11:00:00Z
modified: 2016-02-12T09:06:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2077_
m user id: 4206
owner id: 24524
owning group id: 9
status: status/Planning
bookmark: false
mutable description: to be updated
mutable notes: to be updated
mutable provenance: to be updated
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: start
sequence annotation: Hisx6
sequence annotation: DspBx
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_K1659210_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation2449117
display id: annotation2449117
version: 1
top level: DspBx
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1659210/annotation2449117/range2449117/1

https://synbiohub.org/public/igem/BBa_K1659210/annotation2449117/range2449117/1
type: Range
persistent identity: range2449117
display id: range2449117
version: 1
direction: direction/forward
top level: DspBx
start: 1
end: 3
orientation: inline

Hisx6
type: SequenceAnnotation
persistent identity: annotation2449119
display id: annotation2449119
version: 1
top level: DspBx
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1659210/annotation2449119/range2449119/1

https://synbiohub.org/public/igem/BBa_K1659210/annotation2449119/range2449119/1
type: Range
persistent identity: range2449119
display id: range2449119
version: 1
direction: direction/forward
top level: DspBx
start: 1087
end: 1104
orientation: inline

DspBx
type: SequenceAnnotation
persistent identity: annotation2449118
display id: annotation2449118
version: 1
top level: DspBx
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1659210/annotation2449118/range2449118/1

https://synbiohub.org/public/igem/BBa_K1659210/annotation2449118/range2449118/1
type: Range
persistent identity: range2449118
display id: range2449118
version: 1
direction: direction/forward
top level: DspBx
start: 4
end: 1086
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2453187
display id: annotation2453187
version: 1
top level: DspBx
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1659210/annotation2453187/range2453187/1

https://synbiohub.org/public/igem/BBa_K1659210/annotation2453187/range2453187/1
type: Range
persistent identity: range2453187
display id: range2453187
version: 1
direction: direction/forward
top level: DspBx
start: 1105
end: 1110
orientation: inline

BBa_K1399007
type: ComponentDefinition
persistent identity: BBa K1399007
display id: BBa_K1399007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP (mut3b) with SsrA-DAS+2 degradation tag
creator: Anna Stikane
created: 2014-09-18T11:00:00Z
modified: 2015-05-08T01:10:16Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1777_
m user id: 0
owner id: 22477
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: GFP (mut3b) (see part BBa_E0040) with added engineered NYADAS-ssrA degradation tag (see part BBa_M0050). The tag increases GFP turn-over rate, thus providing better temporal resolution of green fluorescence. In the same time, maximal fluorescence amplitudes will be lower as newly formed protein is degraded as soon as it is formed. SsrA tags encode peptide sequence that and is recognized by ClpA and ClpX unfoldases and ClpX mediator SspB.[1] ClpA and ClpX then form a proteosome-like complex with ClpP protease and the protein is degraded.[1] The final three residues of the tag determines the strength of interaction with ClpX and thus the final protein degradation rate.[2] The NYADAS tag encodes peptide sequence AANDENYNYDAS is reported to have low affinity to ClpX thus its mediated degradation very much depends on the concentration of SspB (ClpX mediator).[1] The two additional residues ???NY??? extends tag between SspB and ClpX binding site, thus preventing clash when both these protein are bound to tag.[3] However, be aware that exact protein degradation rate is influenced by multiple other factors: ClpXP and ClpAP protease concentrations, protein stability, Km of binding to the protease, temperature [4]. ===References=== [1] Flynn, J. M. et al. Overlapping recognition determinants within the ssrA degradation tag allow modulation of proteolysis. Proc. Natl. Acad. Sci. U. S. A. 98, 10584???9 (2001). [2] Andersen, J. B. et al. New unstable variants of green fluorescent protein for studies of transient gene expression in bacteria. Appl. Environ. Microbiol. 64, 2240???6 (1998). [3] McGinness, K. E., Baker, T. a & Sauer, R. T. Engineering controllable protein degradation. Mol. Cell 22, 701???7 (2006). [4] Purcell, O., Grierson, C. S., Bernardo, M. Di & Savery, N. J. Temperature dependence of ssrA-tag mediated protein degradation. J. Biol. Eng. 6, 10 (2012).
mutable notes: The tag was attached to GFP using PCR and MABEL (mutagenesis with blunt-end ligation), thus avoiding introduction of additional residues and restriction site. Different parts of the tag are recognized by different proteins, for example, the final 3 residues (DAS in this case) are recognized by ClpX, whereas first 4 residues of the tag are required for efficient SspB binding.[1] Thus modifications of these critical residues alter the efficacy with what different proteases bind to it.
mutable provenance: GFP comes from part BBa_E0040, the tag sequence was obtained from part BBa_M0051.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: stop
sequence annotation: start
sequence annotation: NYADAS-ssrA
sequence annotation: GFP (mut3b)
sequence: https://synbiohub.org/public/igem/BBa_K1399007_sequence/1

stop
type: SequenceAnnotation
persistent identity: annotation2383926
display id: annotation2383926
version: 1
top level: BBa_K1399007
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399007/annotation2383926/range2383926/1

https://synbiohub.org/public/igem/BBa_K1399007/annotation2383926/range2383926/1
type: Range
persistent identity: range2383926
display id: range2383926
version: 1
direction: direction/forward
top level: BBa_K1399007
start: 754
end: 756
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2383923
display id: annotation2383923
version: 1
top level: BBa_K1399007
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1399007/annotation2383923/range2383923/1

https://synbiohub.org/public/igem/BBa_K1399007/annotation2383923/range2383923/1
type: Range
persistent identity: range2383923
display id: range2383923
version: 1
direction: direction/forward
top level: BBa_K1399007
start: 1
end: 3
orientation: inline

NYADAS-ssrA
type: SequenceAnnotation
persistent identity: annotation2383925
display id: annotation2383925
version: 1
top level: BBa_K1399007
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1399007/annotation2383925/range2383925/1

https://synbiohub.org/public/igem/BBa_K1399007/annotation2383925/range2383925/1
type: Range
persistent identity: range2383925
display id: range2383925
version: 1
direction: direction/forward
top level: BBa_K1399007
start: 715
end: 753
orientation: inline

GFP (mut3b)
type: SequenceAnnotation
persistent identity: annotation2383924
display id: annotation2383924
version: 1
top level: BBa_K1399007
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1399007/annotation2383924/range2383924/1

https://synbiohub.org/public/igem/BBa_K1399007/annotation2383924/range2383924/1
type: Range
persistent identity: range2383924
display id: range2383924
version: 1
direction: direction/forward
top level: BBa_K1399007
start: 4
end: 714
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2383927
display id: annotation2383927
version: 1
top level: BBa_K1399007
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399007/annotation2383927/range2383927/1

https://synbiohub.org/public/igem/BBa_K1399007/annotation2383927/range2383927/1
type: Range
persistent identity: range2383927
display id: range2383927
version: 1
direction: direction/forward
top level: BBa_K1399007
start: 757
end: 759
orientation: inline

BBa_K1789008
type: ComponentDefinition
persistent identity: BBa K1789008
display id: BBa_K1789008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TALE1
creator: Xinyuan Qiu
created: 2015-09-13T11:00:00Z
modified: 2015-09-18T02:27:57Z
discontinued: false
dominant: false
group u list: _2214_
m user id: 21134
owner id: 21134
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This is a engineered TAL effector that can bind with the DNA sequence 5'-GGAGGCACCGGTGG-3'
mutable notes: The part sequence should be able to correctly and efficiently bind with corresponding DNA, so we used bioinformatics tools to help us designing this sequence.
mutable provenance: This part is built by NJU_CHINA using GATEWAY technique. We standardized this part by ourselves.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: TALE1
sequence annotation: DNA Binding domain
sequence: https://synbiohub.org/public/igem/BBa_K1789008_sequence/1

TALE1
type: SequenceAnnotation
persistent identity: annotation2454114
display id: annotation2454114
version: 1
top level: BBa_K1789008
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1789008/annotation2454114/range2454114/1

https://synbiohub.org/public/igem/BBa_K1789008/annotation2454114/range2454114/1
type: Range
persistent identity: range2454114
display id: range2454114
version: 1
direction: direction/forward
top level: BBa_K1789008
start: 1
end: 2087
orientation: inline

DNA Binding domain
type: SequenceAnnotation
persistent identity: annotation2454113
display id: annotation2454113
version: 1
top level: BBa_K1789008
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1789008/annotation2454113/range2454113/1

https://synbiohub.org/public/igem/BBa_K1789008/annotation2454113/range2454113/1
type: Range
persistent identity: range2454113
display id: range2454113
version: 1
direction: direction/forward
top level: BBa_K1789008
start: 508
end: 1872
orientation: inline

BBa_K1491009
type: ComponentDefinition
persistent identity: BBa K1491009
display id: BBa_K1491009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS-mRFP-His
creator: Danielle Peters
created: 2014-07-14T11:00:00Z
modified: 2015-05-08T01:10:44Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1871_
m user id: 0
owner id: 22521
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: mRFP with 6x His tag (BBa_K1491008) and a strong RBS (BBa_B0034). It can be used as a reporter.
mutable notes: RBS+RFP+His
mutable provenance: RBS is BBa_B0034 and mRFP is BBa_E1010
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_K1491008
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_K1491008
sequence: https://synbiohub.org/public/igem/BBa_K1491009_sequence/1

BBa_K1491008
type: Component
persistent identity: component2380114
display id: component2380114
version: 1
top level: BBa_K1491009
access: public
definition: https://synbiohub.org/public/igem/BBa_K1491008/1

BBa_B0034
type: Component
persistent identity: component2380111
display id: component2380111
version: 1
top level: BBa_K1491009
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2380111
display id: annotation2380111
version: 1
top level: BBa_K1491009
location: https://synbiohub.org/public/igem/BBa_K1491009/annotation2380111/range2380111/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1491009/annotation2380111/range2380111/1
type: Range
persistent identity: range2380111
display id: range2380111
version: 1
direction: direction/reverse
top level: BBa_K1491009
start: 1
end: 12
orientation: inline

BBa_K1491008
type: SequenceAnnotation
persistent identity: annotation2380114
display id: annotation2380114
version: 1
top level: BBa_K1491009
location: https://synbiohub.org/public/igem/BBa_K1491009/annotation2380114/range2380114/1
component: BBa_K1491008

https://synbiohub.org/public/igem/BBa_K1491009/annotation2380114/range2380114/1
type: Range
persistent identity: range2380114
display id: range2380114
version: 1
direction: direction/reverse
top level: BBa_K1491009
start: 19
end: 717
orientation: inline

BBa_K1763441
type: ComponentDefinition
persistent identity: BBa K1763441
display id: BBa_K1763441
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: MaSp1/2[1:1]-12(T7)
creator: Vinson Lam
created: 2015-09-14T11:00:00Z
modified: 2015-09-15T09:54:37Z
discontinued: false
dominant: false
group u list: _2185_
m user id: 27136
owner id: 27136
owning group id: 9
status: status/Planning
bookmark: false
mutable description: MaSp1 and MaSp2 12-mer block co-polymer under control of T7 promoter and RBS. Ratio of 1 MaSp1 to 1 MaSp2.
mutable notes: no.
mutable provenance: N. clavipes
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K1763440
component: BBa_K525998
sequence annotation: BBa_K525998
sequence annotation: BBa_K1763440
sequence: https://synbiohub.org/public/igem/BBa_K1763441_sequence/1

BBa_K1763440
type: Component
persistent identity: component2456991
display id: component2456991
version: 1
top level: BBa_K1763441
access: public
definition: BBa_K1763440

BBa_K525998
type: Component
persistent identity: component2456990
display id: component2456990
version: 1
top level: BBa_K1763441
access: public
definition: https://synbiohub.org/public/igem/BBa_K525998/1

BBa_K525998
type: SequenceAnnotation
persistent identity: annotation2456990
display id: annotation2456990
version: 1
top level: BBa_K1763441
location: https://synbiohub.org/public/igem/BBa_K1763441/annotation2456990/range2456990/1
component: BBa_K525998

https://synbiohub.org/public/igem/BBa_K1763441/annotation2456990/range2456990/1
type: Range
persistent identity: range2456990
display id: range2456990
version: 1
direction: direction/reverse
top level: BBa_K1763441
start: 1
end: 32
orientation: inline

BBa_K1763440
type: SequenceAnnotation
persistent identity: annotation2456991
display id: annotation2456991
version: 1
top level: BBa_K1763441
location: https://synbiohub.org/public/igem/BBa_K1763441/annotation2456991/range2456991/1
component: BBa_K1763440

https://synbiohub.org/public/igem/BBa_K1763441/annotation2456991/range2456991/1
type: Range
persistent identity: range2456991
display id: range2456991
version: 1
direction: direction/reverse
top level: BBa_K1763441
start: 39
end: 1313
orientation: inline

BBa_K1602051
type: ComponentDefinition
persistent identity: BBa K1602051
display id: BBa_K1602051
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: araCpBad-RRkey
creator: Christian Sator,Stefan Zens, Max Zander, Benedikt Spannenkrebs, Sebastian Jaeger
created: 2015-09-15T11:00:00Z
modified: 2015-09-25T05:11:55Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _2019_
m user id: 23028
owner id: 26220
owning group id: 9
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1602049
component: BBa_K808000
sequence annotation: BBa_K1602049
sequence annotation: BBa_K808000
sequence: https://synbiohub.org/public/igem/BBa_K1602051_sequence/1

BBa_K1602049
type: Component
persistent identity: component2462790
display id: component2462790
version: 1
top level: BBa_K1602051
access: public
definition: BBa_K1602049

BBa_K808000
type: Component
persistent identity: component2462789
display id: component2462789
version: 1
top level: BBa_K1602051
access: public
definition: https://synbiohub.org/public/igem/BBa_K808000/1

BBa_K1602049
type: SequenceAnnotation
persistent identity: annotation2462790
display id: annotation2462790
version: 1
top level: BBa_K1602051
location: https://synbiohub.org/public/igem/BBa_K1602051/annotation2462790/range2462790/1
component: BBa_K1602049

https://synbiohub.org/public/igem/BBa_K1602051/annotation2462790/range2462790/1
type: Range
persistent identity: range2462790
display id: range2462790
version: 1
direction: direction/reverse
top level: BBa_K1602051
start: 1218
end: 1288
orientation: inline

BBa_K808000
type: SequenceAnnotation
persistent identity: annotation2462789
display id: annotation2462789
version: 1
top level: BBa_K1602051
location: https://synbiohub.org/public/igem/BBa_K1602051/annotation2462789/range2462789/1
component: BBa_K808000

https://synbiohub.org/public/igem/BBa_K1602051/annotation2462789/range2462789/1
type: Range
persistent identity: range2462789
display id: range2462789
version: 1
direction: direction/reverse
top level: BBa_K1602051
start: 1
end: 1209
orientation: inline

ldhE-Lc
type: ComponentDefinition
persistent identity: BBa K1696001
display id: BBa_K1696001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: L-Lactate producing module from Lactobacillus casei
creator: Yue Liu
created: 2015-09-10T11:00:00Z
modified: 2015-09-11T10:20:43Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2114_
m user id: 16881
owner id: 16881
owning group id: 9
status: status/Planning
bookmark: false
mutable description: The ldh gene can produce L-lactate from pyruvate, which not only increase the overall amounts of lactate but reduce the byproduct generation.
mutable notes: coden optimization
mutable provenance: From Lactobacillus casei
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: ldhA
sequence: https://synbiohub.org/public/igem/BBa_K1696001_sequence/1

ldhA
type: SequenceAnnotation
persistent identity: annotation2449232
display id: annotation2449232
version: 1
top level: ldhE-Lc
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1696001/annotation2449232/range2449232/1

https://synbiohub.org/public/igem/BBa_K1696001/annotation2449232/range2449232/1
type: Range
persistent identity: range2449232
display id: range2449232
version: 1
direction: direction/forward
top level: ldhE-Lc
start: 1
end: 981
orientation: inline

mLacI
type: ComponentDefinition
persistent identity: BBa K1639010
display id: BBa_K1639010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: mammalian LacI with miR-373 binding site
creator: Nurgeldi Bazarov
created: 2015-09-14T11:00:00Z
modified: 2015-09-15T12:20:03Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2056_
m user id: 24789
owner id: 24789
owning group id: 9
status: status/Planning
bookmark: false
mutable description: In our project second step of cancer module is based on production of LacI so that it will suppress expression of DsRed. Binding site on 3' end of LacI makes it regulatable and limits it's expression only in cells with low miR-373 rate. In gastric epithelial cells level of this mirna is low but in gastric cancer cells level is very high.
mutable notes: We inserted two Mlu1 cut sites on each side of miR-373 binding so that other iGEM teams could easily replace it with any sequence.
mutable provenance: This protein is mammalian codon optimized version of LacI repressor originally derived from E.coli. It also possess nuclear localization signal (NLS) so that it can act on lac operators.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Stop Codon
sequence annotation: miR-373 binding site
sequence annotation: Kozak
sequence annotation: mammalian LacI
sequence annotation: ATG
sequence annotation: SV40 NLS
sequence: https://synbiohub.org/public/igem/BBa_K1639010_sequence/1

Stop Codon
type: SequenceAnnotation
persistent identity: annotation2457337
display id: annotation2457337
version: 1
top level: mLacI
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1639010/annotation2457337/range2457337/1

https://synbiohub.org/public/igem/BBa_K1639010/annotation2457337/range2457337/1
type: Range
persistent identity: range2457337
display id: range2457337
version: 1
direction: direction/forward
top level: mLacI
start: 1123
end: 1134
orientation: inline

miR-373 binding site
type: SequenceAnnotation
persistent identity: annotation2457338
display id: annotation2457338
version: 1
top level: mLacI
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1639010/annotation2457338/range2457338/1

https://synbiohub.org/public/igem/BBa_K1639010/annotation2457338/range2457338/1
type: Range
persistent identity: range2457338
display id: range2457338
version: 1
direction: direction/forward
top level: mLacI
start: 1141
end: 1163
orientation: inline

Kozak
type: SequenceAnnotation
persistent identity: annotation2457312
display id: annotation2457312
version: 1
top level: mLacI
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1639010/annotation2457312/range2457312/1

https://synbiohub.org/public/igem/BBa_K1639010/annotation2457312/range2457312/1
type: Range
persistent identity: range2457312
display id: range2457312
version: 1
direction: direction/forward
top level: mLacI
start: 1
end: 6
orientation: inline

mammalian LacI
type: SequenceAnnotation
persistent identity: annotation2457315
display id: annotation2457315
version: 1
top level: mLacI
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1639010/annotation2457315/range2457315/1

https://synbiohub.org/public/igem/BBa_K1639010/annotation2457315/range2457315/1
type: Range
persistent identity: range2457315
display id: range2457315
version: 1
direction: direction/forward
top level: mLacI
start: 7
end: 1125
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2457339
display id: annotation2457339
version: 1
top level: mLacI
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1639010/annotation2457339/range2457339/1

https://synbiohub.org/public/igem/BBa_K1639010/annotation2457339/range2457339/1
type: Range
persistent identity: range2457339
display id: range2457339
version: 1
direction: direction/forward
top level: mLacI
start: 7
end: 9
orientation: inline

SV40 NLS
type: SequenceAnnotation
persistent identity: annotation2457336
display id: annotation2457336
version: 1
top level: mLacI
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1639010/annotation2457336/range2457336/1

https://synbiohub.org/public/igem/BBa_K1639010/annotation2457336/range2457336/1
type: Range
persistent identity: range2457336
display id: range2457336
version: 1
direction: direction/forward
top level: mLacI
start: 1102
end: 1122
orientation: inline

BBa_K1321151
type: ComponentDefinition
persistent identity: BBa K1321151
display id: BBa_K1321151
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: metallothionein Fmt fused to CBDclos
creator: Gabriella Santosa
created: 2014-10-08T11:00:00Z
modified: 2015-06-17T12:23:26Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 4206
owner id: 20961
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: GATC
mutable notes: GATC
mutable provenance: GATC
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1321012
component: BBa_K1321002
component: BBa_B0105
sequence annotation: BBa_K1321002
sequence annotation: BBa_K1321012
sequence annotation: BBa_B0105
sequence: https://synbiohub.org/public/igem/BBa_K1321151_sequence/1

BBa_K1321012
type: Component
persistent identity: component2415027
display id: component2415027
version: 1
top level: BBa_K1321151
access: public
definition: https://synbiohub.org/public/igem/BBa_K1321012/1

BBa_K1321002
type: Component
persistent identity: component2415030
display id: component2415030
version: 1
top level: BBa_K1321151
access: public
definition: https://synbiohub.org/public/igem/BBa_K1321002/1

BBa_B0105
type: Component
persistent identity: component2415029
display id: component2415029
version: 1
top level: BBa_K1321151
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_K1321002
type: SequenceAnnotation
persistent identity: annotation2415030
display id: annotation2415030
version: 1
top level: BBa_K1321151
location: https://synbiohub.org/public/igem/BBa_K1321151/annotation2415030/range2415030/1
component: BBa_K1321002

https://synbiohub.org/public/igem/BBa_K1321151/annotation2415030/range2415030/1
type: Range
persistent identity: range2415030
display id: range2415030
version: 1
direction: direction/reverse
top level: BBa_K1321151
start: 205
end: 504
orientation: inline

BBa_K1321012
type: SequenceAnnotation
persistent identity: annotation2415027
display id: annotation2415027
version: 1
top level: BBa_K1321151
location: https://synbiohub.org/public/igem/BBa_K1321151/annotation2415027/range2415027/1
component: BBa_K1321012

https://synbiohub.org/public/igem/BBa_K1321151/annotation2415027/range2415027/1
type: Range
persistent identity: range2415027
display id: range2415027
version: 1
direction: direction/reverse
top level: BBa_K1321151
start: 1
end: 198
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2415029
display id: annotation2415029
version: 1
top level: BBa_K1321151
location: https://synbiohub.org/public/igem/BBa_K1321151/annotation2415029/range2415029/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_K1321151/annotation2415029/range2415029/1
type: Range
persistent identity: range2415029
display id: range2415029
version: 1
direction: direction/reverse
top level: BBa_K1321151
start: 199
end: 204
orientation: inline

BBa_K1653006
type: ComponentDefinition
persistent identity: BBa K1653006
display id: BBa_K1653006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: MarA
creator: Yoshiharu Otaki, Daiki Haraguchi
created: 2015-05-30T11:00:00Z
modified: 2015-09-18T04:00:50Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2071_
m user id: 22434
owner id: 22434
owning group id: 9
status: status/Planning
bookmark: false
mutable description: next time
mutable notes: next time
mutable provenance: next time
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: MarA
sequence: https://synbiohub.org/public/igem/BBa_K1653006_sequence/1

MarA
type: SequenceAnnotation
persistent identity: annotation2432405
display id: annotation2432405
version: 1
top level: BBa_K1653006
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1653006/annotation2432405/range2432405/1

https://synbiohub.org/public/igem/BBa_K1653006/annotation2432405/range2432405/1
type: Range
persistent identity: range2432405
display id: range2432405
version: 1
direction: direction/forward
top level: BBa_K1653006
start: 1
end: 384
orientation: inline

BBa_K1689014
type: ComponentDefinition
persistent identity: BBa K1689014
display id: BBa_K1689014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: dCas9-Clact
creator: Wei weijia
created: 2015-09-15T11:00:00Z
modified: 2015-09-27T01:31:12Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2107_
m user id: 26531
owner id: 26531
owning group id: 9
status: status/Planning
bookmark: false
mutable description: pSB1C3-6H-dCas9-Clact, C terminal segment of beta-lacatamse fused with dCas9, His tag allows for protein purification
mutable notes: delta alpha segment of LacZ fused with dCas9, His tag allows for protein purification
mutable provenance: E.coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: dCas9
sequence annotation: random spacer
sequence annotation: 6??His tag
sequence annotation: dCas9-Clact
sequence annotation: GGGGS*2 Linker
sequence annotation: stop
sequence annotation: C terminal of β-Lactamase
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_K1689014_sequence/1

dCas9
type: SequenceAnnotation
persistent identity: annotation2469006
display id: annotation2469006
version: 1
top level: BBa_K1689014
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1689014/annotation2469006/range2469006/1

https://synbiohub.org/public/igem/BBa_K1689014/annotation2469006/range2469006/1
type: Range
persistent identity: range2469006
display id: range2469006
version: 1
direction: direction/forward
top level: BBa_K1689014
start: 55
end: 4155
orientation: inline

random spacer
type: SequenceAnnotation
persistent identity: annotation2477780
display id: annotation2477780
version: 1
top level: BBa_K1689014
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1689014/annotation2477780/range2477780/1

https://synbiohub.org/public/igem/BBa_K1689014/annotation2477780/range2477780/1
type: Range
persistent identity: range2477780
display id: range2477780
version: 1
direction: direction/forward
top level: BBa_K1689014
start: 4462
end: 4532
orientation: inline

6??His tag
type: SequenceAnnotation
persistent identity: annotation2469003
display id: annotation2469003
version: 1
top level: BBa_K1689014
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1689014/annotation2469003/range2469003/1

https://synbiohub.org/public/igem/BBa_K1689014/annotation2469003/range2469003/1
type: Range
persistent identity: range2469003
display id: range2469003
version: 1
direction: direction/forward
top level: BBa_K1689014
start: 37
end: 54
orientation: inline

dCas9-Clact
type: SequenceAnnotation
persistent identity: annotation2470120
display id: annotation2470120
version: 1
top level: BBa_K1689014
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1689014/annotation2470120/range2470120/1

https://synbiohub.org/public/igem/BBa_K1689014/annotation2470120/range2470120/1
type: Range
persistent identity: range2470120
display id: range2470120
version: 1
direction: direction/forward
top level: BBa_K1689014
start: 34
end: 4461
orientation: inline

GGGGS*2 Linker
type: SequenceAnnotation
persistent identity: annotation2469010
display id: annotation2469010
version: 1
top level: BBa_K1689014
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1689014/annotation2469010/range2469010/1

https://synbiohub.org/public/igem/BBa_K1689014/annotation2469010/range2469010/1
type: Range
persistent identity: range2469010
display id: range2469010
version: 1
direction: direction/forward
top level: BBa_K1689014
start: 4156
end: 4185
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2477779
display id: annotation2477779
version: 1
top level: BBa_K1689014
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1689014/annotation2477779/range2477779/1

https://synbiohub.org/public/igem/BBa_K1689014/annotation2477779/range2477779/1
type: Range
persistent identity: range2477779
display id: range2477779
version: 1
direction: direction/forward
top level: BBa_K1689014
start: 4459
end: 4461
orientation: inline

random spacer
type: SequenceAnnotation
persistent identity: annotation2477777
display id: annotation2477777
version: 1
top level: BBa_K1689014
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1689014/annotation2477777/range2477777/1

https://synbiohub.org/public/igem/BBa_K1689014/annotation2477777/range2477777/1
type: Range
persistent identity: range2477777
display id: range2477777
version: 1
direction: direction/forward
top level: BBa_K1689014
start: 1
end: 33
orientation: inline

C terminal of β-Lactamase
type: SequenceAnnotation
persistent identity: annotation2469012
display id: annotation2469012
version: 1
top level: BBa_K1689014
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1689014/annotation2469012/range2469012/1

https://synbiohub.org/public/igem/BBa_K1689014/annotation2469012/range2469012/1
type: Range
persistent identity: range2469012
display id: range2469012
version: 1
direction: direction/forward
top level: BBa_K1689014
start: 4186
end: 4458
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2477778
display id: annotation2477778
version: 1
top level: BBa_K1689014
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1689014/annotation2477778/range2477778/1

https://synbiohub.org/public/igem/BBa_K1689014/annotation2477778/range2477778/1
type: Range
persistent identity: range2477778
display id: range2477778
version: 1
direction: direction/forward
top level: BBa_K1689014
start: 34
end: 36
orientation: inline

pBar1
type: ComponentDefinition
persistent identity: BBa K1829001
display id: BBa_K1829001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pBar1: Mating factor alpha responsive promoter
creator: Nicholas Suen
created: 2015-09-07T11:00:00Z
modified: 2015-09-14T11:39:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2255_
m user id: 18557
owner id: 27842
owning group id: 9
status: status/Planning
bookmark: false
mutable description: The pBar1 promoter is a mating factor alpha responsive promoter found in MATa S. Cerevisiae. This promoter drives expression of Bar1, a protease that cleaves mating factor alpha.
mutable notes: We changed nucleotide 210 from thymine to cytosine. This does not change the amino acid Asparagine and is therefore a silent mutation. We also changed nucleotide 285 from cytosine to adenine. This does not change the amino acid valine and is therefore a silent mutation.
mutable provenance: pBar1 is found in the genomic sequence of MATa S. cerevisiae.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K1829001_sequence/1

UNIK Antif
type: ComponentDefinition
persistent identity: BBa K1825000
display id: BBa_K1825000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: UNIK antifreeze protein
creator: Adam Petersen
created: 2015-08-30T11:00:00Z
modified: 2016-01-27T10:30:03Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2251_
m user id: 4206
owner id: 28032
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This is the coding region of a small antifreeze protein. It works by binding to the ice crystals as they form inside the cell, and by doing so prevents the ice crystals from enlarging and penetrating the cell membrane. We wanted to test if there would be a significant difference in the growth rate of moss, Physcomitrella patens, with the antifreeze gene, compared to the one without. This would be done at a low temperature to test the in-vivo function of the antifreeze protein.
mutable notes: We used codon optimization for moss so it would work better in Physcomitrella Patens.
mutable provenance: The part is taken from a spruce budworm, Choristoneura fumiferana, living in areas with permafrost.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: startcodon
sequence: https://synbiohub.org/public/igem/BBa_K1825000_sequence/1

startcodon
type: SequenceAnnotation
persistent identity: annotation2440844
display id: annotation2440844
version: 1
top level: UNIK Antif
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1825000/annotation2440844/range2440844/1

https://synbiohub.org/public/igem/BBa_K1825000/annotation2440844/range2440844/1
type: Range
persistent identity: range2440844
display id: range2440844
version: 1
direction: direction/forward
top level: UNIK Antif
start: 1
end: 3
orientation: inline

BBa_K1585312
type: ComponentDefinition
persistent identity: BBa K1585312
display id: BBa_K1585312
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0034.GlgC
creator: Michael Osthege, Sarah Lubjuhn, Laura Helleckes
created: 2015-08-24T11:00:00Z
modified: 2015-09-17T02:21:03Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2002_
m user id: 24474
owner id: 24505
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This is the translational unit of GlgC. It creates ADP-glucose from glucose-1-phosphate for glycogen synthesis.
mutable notes: -
mutable provenance: E. coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0034
component: BBa_K118016
sequence annotation: BBa_B0034
sequence annotation: BBa_K118016
sequence: https://synbiohub.org/public/igem/BBa_K1585312_sequence/1

BBa_B0034
type: Component
persistent identity: component2437914
display id: component2437914
version: 1
top level: BBa_K1585312
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K118016
type: Component
persistent identity: component2437918
display id: component2437918
version: 1
top level: BBa_K1585312
access: public
definition: https://synbiohub.org/public/igem/BBa_K118016/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2437914
display id: annotation2437914
version: 1
top level: BBa_K1585312
location: https://synbiohub.org/public/igem/BBa_K1585312/annotation2437914/range2437914/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1585312/annotation2437914/range2437914/1
type: Range
persistent identity: range2437914
display id: range2437914
version: 1
direction: direction/reverse
top level: BBa_K1585312
start: 1
end: 12
orientation: inline

BBa_K118016
type: SequenceAnnotation
persistent identity: annotation2437918
display id: annotation2437918
version: 1
top level: BBa_K1585312
location: https://synbiohub.org/public/igem/BBa_K1585312/annotation2437918/range2437918/1
component: BBa_K118016

https://synbiohub.org/public/igem/BBa_K1585312/annotation2437918/range2437918/1
type: Range
persistent identity: range2437918
display id: range2437918
version: 1
direction: direction/reverse
top level: BBa_K1585312
start: 19
end: 1317
orientation: inline

BBa_K1585310
type: ComponentDefinition
persistent identity: BBa K1585310
display id: BBa_K1585310
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0034.GlgA
creator: Laura Helleckes, Michael Osthege, Sarah Lubjuhn
created: 2015-08-24T11:00:00Z
modified: 2015-09-17T02:20:38Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2002_
m user id: 24474
owner id: 24505
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This is the translational unit of GlgA (Glycogen synthase) for elongation of glycogen chains. Through autophosphorylation, GlgA is also the starting point of glycogen synthesis in bacteria.
mutable notes: -
mutable provenance: E. coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0034
component: BBa_K1585300
sequence annotation: BBa_K1585300
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K1585310_sequence/1

BBa_B0034
type: Component
persistent identity: component2437901
display id: component2437901
version: 1
top level: BBa_K1585310
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1585300
type: Component
persistent identity: component2437909
display id: component2437909
version: 1
top level: BBa_K1585310
access: public
definition: https://synbiohub.org/public/igem/BBa_K1585300/1

BBa_K1585300
type: SequenceAnnotation
persistent identity: annotation2437909
display id: annotation2437909
version: 1
top level: BBa_K1585310
location: https://synbiohub.org/public/igem/BBa_K1585310/annotation2437909/range2437909/1
component: BBa_K1585300

https://synbiohub.org/public/igem/BBa_K1585310/annotation2437909/range2437909/1
type: Range
persistent identity: range2437909
display id: range2437909
version: 1
direction: direction/reverse
top level: BBa_K1585310
start: 19
end: 1455
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2437901
display id: annotation2437901
version: 1
top level: BBa_K1585310
location: https://synbiohub.org/public/igem/BBa_K1585310/annotation2437901/range2437901/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1585310/annotation2437901/range2437901/1
type: Range
persistent identity: range2437901
display id: range2437901
version: 1
direction: direction/reverse
top level: BBa_K1585310
start: 1
end: 12
orientation: inline

1018
type: ComponentDefinition
persistent identity: BBa K1344000
display id: BBa_K1344000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 1018 synthetic cationic peptide
creator: UI Indonesia 2014
created: 2014-10-03T11:00:00Z
modified: 2015-05-08T01:10:00Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _1719_
m user id: 0
owner id: 20150
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: Peptide 1018 is a synthetic cationic peptides, derived from natural peptides such as the human cathelicidin LL-37 and the bovine peptide indolicidin. This part work as biofilm inhibitory compounds. Anti-biofilm peptides are similar to cationic antimicrobial peptides (which are active against planktonic bacteria), comprising both cationic and hydrophobic amino acids.
mutable notes: This part will have broad spectrum effect on gram-negative and gram-positive bacterias
mutable provenance: This part is synthetized.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: 1018
sequence annotation: Stop
sequence annotation: ATG
sequence: https://synbiohub.org/public/igem/BBa_K1344000_sequence/1

1018
type: SequenceAnnotation
persistent identity: annotation2393869
display id: annotation2393869
version: 1
top level: 1018
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1344000/annotation2393869/range2393869/1

https://synbiohub.org/public/igem/BBa_K1344000/annotation2393869/range2393869/1
type: Range
persistent identity: range2393869
display id: range2393869
version: 1
direction: direction/forward
top level: 1018
start: 1
end: 45
orientation: inline

Stop
type: SequenceAnnotation
persistent identity: annotation2393870
display id: annotation2393870
version: 1
top level: 1018
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1344000/annotation2393870/range2393870/1

https://synbiohub.org/public/igem/BBa_K1344000/annotation2393870/range2393870/1
type: Range
persistent identity: range2393870
display id: range2393870
version: 1
direction: direction/forward
top level: 1018
start: 40
end: 45
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2393871
display id: annotation2393871
version: 1
top level: 1018
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1344000/annotation2393871/range2393871/1

https://synbiohub.org/public/igem/BBa_K1344000/annotation2393871/range2393871/1
type: Range
persistent identity: range2393871
display id: range2393871
version: 1
direction: direction/forward
top level: 1018
start: 1
end: 1
orientation: inline

Dsb-Art175
type: ComponentDefinition
persistent identity: BBa K1659002
display id: BBa_K1659002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Artilysin Art-175 fused at N-terminal with DsbA signal peptide
creator: Wei Chung Kong
created: 2015-08-25T11:00:00Z
modified: 2016-02-01T12:52:09Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2077_
m user id: 4206
owner id: 24524
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Artilysins are an exciting class of enzyme-based antibacterials. Their name is derived from "artificial endolysin" and they exploit the lytic power of bacteriophage-encoded endolysins. Endolysins are peptidoglycan hydrolases produced at the end of the lytic cycle that pass through the cytoplasmic membrane, degrade the peptidoglycan layer and cause the osmotic lysis of the infected bacterial cell, thus liberating the progeny. Purified endolysins have been used to kill Gram-positive pathogens. Gram-negative bacteria, however, have a protective outer membrane containing lipopolysaccharide (LPS) that serves as a barrier against the peptidoglycan hydrolytic activity of endolysins from the outside. To overcome this problem, selected polycationic or amphipathic peptides that locally destabilize the LPS layer have been covalently fused to endolysins. Biers et al. fused the sheep myeloid antimicrobial peptide (SMAP-29) to the N terminus of the endolysin KZ144 to create Artilysin Art-175. Art-175 has been shown to be a highly potent antibacterial that acts in minutes to kill virtually all Pseudomonas aeruginosa strains. [2] BBa_K1659002 is a composite of Art-175 with the N-terminal DsbA signal peptide with a hexahistidine tag. DsbA targets recombinant proteins to the co-translational signal- recognition-particle (SRP)-dependent pathway to mediate secretion out of the bacterial chassis. [2]
mutable notes: -
mutable provenance: Endolysin KZ144 originates from Pseudomonas aeruginosa bacteriophage varphiKZ. SMAP-29 originates from sheep genomic DNA.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: Hisx6
sequence annotation: Art-175
sequence annotation: stop
sequence annotation: start
sequence annotation: DsbA 2-19 Signal Sequence
sequence: https://synbiohub.org/public/igem/BBa_K1659002_sequence/1

Hisx6
type: SequenceAnnotation
persistent identity: annotation2445688
display id: annotation2445688
version: 1
top level: Dsb-Art175
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1659002/annotation2445688/range2445688/1

https://synbiohub.org/public/igem/BBa_K1659002/annotation2445688/range2445688/1
type: Range
persistent identity: range2445688
display id: range2445688
version: 1
direction: direction/forward
top level: Dsb-Art175
start: 925
end: 942
orientation: inline

Art-175
type: SequenceAnnotation
persistent identity: annotation2445687
display id: annotation2445687
version: 1
top level: Dsb-Art175
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1659002/annotation2445687/range2445687/1

https://synbiohub.org/public/igem/BBa_K1659002/annotation2445687/range2445687/1
type: Range
persistent identity: range2445687
display id: range2445687
version: 1
direction: direction/forward
top level: Dsb-Art175
start: 58
end: 924
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2445689
display id: annotation2445689
version: 1
top level: Dsb-Art175
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1659002/annotation2445689/range2445689/1

https://synbiohub.org/public/igem/BBa_K1659002/annotation2445689/range2445689/1
type: Range
persistent identity: range2445689
display id: range2445689
version: 1
direction: direction/forward
top level: Dsb-Art175
start: 943
end: 948
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2445685
display id: annotation2445685
version: 1
top level: Dsb-Art175
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1659002/annotation2445685/range2445685/1

https://synbiohub.org/public/igem/BBa_K1659002/annotation2445685/range2445685/1
type: Range
persistent identity: range2445685
display id: range2445685
version: 1
direction: direction/forward
top level: Dsb-Art175
start: 1
end: 3
orientation: inline

DsbA 2-19 Signal Sequence
type: SequenceAnnotation
persistent identity: annotation2445686
display id: annotation2445686
version: 1
top level: Dsb-Art175
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1659002/annotation2445686/range2445686/1

https://synbiohub.org/public/igem/BBa_K1659002/annotation2445686/range2445686/1
type: Range
persistent identity: range2445686
display id: range2445686
version: 1
direction: direction/forward
top level: Dsb-Art175
start: 4
end: 57
orientation: inline

Yeb-Art175
type: ComponentDefinition
persistent identity: BBa K1659003
display id: BBa_K1659003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Artilysin Art-175 fused at N-terminal with porin-dependent YebF secretion signal protein
creator: Wei Chung Kong
created: 2015-08-27T11:00:00Z
modified: 2016-02-01T12:52:17Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2077_
m user id: 4206
owner id: 24524
owning group id: 9
status: status/Planning
bookmark: false
mutable description: to be updated
mutable notes: to be updated
mutable provenance: to be updated
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: Art-175
sequence annotation: YebF
sequence annotation: stop
sequence annotation: start
sequence annotation: Hisx6
sequence: https://synbiohub.org/public/igem/BBa_K1659003_sequence/1

Art-175
type: SequenceAnnotation
persistent identity: annotation2445827
display id: annotation2445827
version: 1
top level: Yeb-Art175
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1659003/annotation2445827/range2445827/1

https://synbiohub.org/public/igem/BBa_K1659003/annotation2445827/range2445827/1
type: Range
persistent identity: range2445827
display id: range2445827
version: 1
direction: direction/forward
top level: Yeb-Art175
start: 355
end: 1221
orientation: inline

YebF
type: SequenceAnnotation
persistent identity: annotation2445826
display id: annotation2445826
version: 1
top level: Yeb-Art175
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1659003/annotation2445826/range2445826/1

https://synbiohub.org/public/igem/BBa_K1659003/annotation2445826/range2445826/1
type: Range
persistent identity: range2445826
display id: range2445826
version: 1
direction: direction/forward
top level: Yeb-Art175
start: 4
end: 354
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2445829
display id: annotation2445829
version: 1
top level: Yeb-Art175
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1659003/annotation2445829/range2445829/1

https://synbiohub.org/public/igem/BBa_K1659003/annotation2445829/range2445829/1
type: Range
persistent identity: range2445829
display id: range2445829
version: 1
direction: direction/forward
top level: Yeb-Art175
start: 1240
end: 1245
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2445825
display id: annotation2445825
version: 1
top level: Yeb-Art175
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1659003/annotation2445825/range2445825/1

https://synbiohub.org/public/igem/BBa_K1659003/annotation2445825/range2445825/1
type: Range
persistent identity: range2445825
display id: range2445825
version: 1
direction: direction/forward
top level: Yeb-Art175
start: 1
end: 3
orientation: inline

Hisx6
type: SequenceAnnotation
persistent identity: annotation2445828
display id: annotation2445828
version: 1
top level: Yeb-Art175
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1659003/annotation2445828/range2445828/1

https://synbiohub.org/public/igem/BBa_K1659003/annotation2445828/range2445828/1
type: Range
persistent identity: range2445828
display id: range2445828
version: 1
direction: direction/forward
top level: Yeb-Art175
start: 1222
end: 1239
orientation: inline

BBa_K1758312
type: ComponentDefinition
persistent identity: BBa K1758312
display id: BBa_K1758312
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Chromium responsive promoter with UTR+RBS and sfGFP
creator: Team Bielefeld-CeBiTec 2015
created: 2015-08-28T11:00:00Z
modified: 2016-01-06T03:35:44Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2180_
m user id: 4206
owner id: 26630
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Chromium responsive promoter with 5?? untremslated region and sfGFP for detection
mutable notes: ...
mutable provenance: Biobrick modified after K1058007
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: T1
sequence annotation: T7-term
sequence annotation: sfGFP
sequence annotation: Cr-promoter
sequence annotation: 5-UTR
sequence: https://synbiohub.org/public/igem/BBa_K1758312_sequence/1

T1
type: SequenceAnnotation
persistent identity: annotation2439959
display id: annotation2439959
version: 1
top level: BBa_K1758312
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1758312/annotation2439959/range2439959/1

https://synbiohub.org/public/igem/BBa_K1758312/annotation2439959/range2439959/1
type: Range
persistent identity: range2439959
display id: range2439959
version: 1
direction: direction/forward
top level: BBa_K1758312
start: 945
end: 1024
orientation: inline

T7-term
type: SequenceAnnotation
persistent identity: annotation2439960
display id: annotation2439960
version: 1
top level: BBa_K1758312
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1758312/annotation2439960/range2439960/1

https://synbiohub.org/public/igem/BBa_K1758312/annotation2439960/range2439960/1
type: Range
persistent identity: range2439960
display id: range2439960
version: 1
direction: direction/forward
top level: BBa_K1758312
start: 1033
end: 1073
orientation: inline

sfGFP
type: SequenceAnnotation
persistent identity: annotation2439958
display id: annotation2439958
version: 1
top level: BBa_K1758312
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1758312/annotation2439958/range2439958/1

https://synbiohub.org/public/igem/BBa_K1758312/annotation2439958/range2439958/1
type: Range
persistent identity: range2439958
display id: range2439958
version: 1
direction: direction/forward
top level: BBa_K1758312
start: 217
end: 936
orientation: inline

Cr-promoter
type: SequenceAnnotation
persistent identity: annotation2439956
display id: annotation2439956
version: 1
top level: BBa_K1758312
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1758312/annotation2439956/range2439956/1

https://synbiohub.org/public/igem/BBa_K1758312/annotation2439956/range2439956/1
type: Range
persistent identity: range2439956
display id: range2439956
version: 1
direction: direction/forward
top level: BBa_K1758312
start: 1
end: 164
orientation: inline

5-UTR
type: SequenceAnnotation
persistent identity: annotation2439957
display id: annotation2439957
version: 1
top level: BBa_K1758312
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1758312/annotation2439957/range2439957/1

https://synbiohub.org/public/igem/BBa_K1758312/annotation2439957/range2439957/1
type: Range
persistent identity: range2439957
display id: range2439957
version: 1
direction: direction/forward
top level: BBa_K1758312
start: 171
end: 216
orientation: inline

BBa_K1689000
type: ComponentDefinition
persistent identity: BBa K1689000
display id: BBa_K1689000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: sgRNA generator
creator: WEI Weijia
created: 2015-09-15T11:00:00Z
modified: 2015-09-26T11:33:57Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2107_
m user id: 4411
owner id: 26464
owning group id: 9
status: status/Planning
bookmark: true
mutable description: including T7 promotor, LacZ a', crRNA (tracrRNA) and T7 terminator.
mutable notes: TACTAGTAGCGGCCGCTGCAGTCCGGCAAAAAAGGGCAAGGTGTCACCACCCTGCCCTTTTTCTTTAAAACCGAAAAGATTACTTCGCGTTATGCAGGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCACAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGCTCGAGGCTTGGATTCTCACCAATAAAAAACGCCCGGCGGCAACCGAGCGTTCTGAACAAATCCAGATGGAGTTCTGAGGTCATTACTGGATCTATCAACAGGAGTCCAAGCGAGCTCGATATCAAATTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCATTAAGCATTCTGCCGACATGGAAGCCATCACAAACGGCATGATGAACCTGAATCGCCAGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAGACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGAAATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCCTGAAAATCTCGATAACTCAAAAAATACGCCCGGTAGTGATCTTATTTCATTATGGTGAAAGTTGGAACCTCTTACGTGCCCGATCAACTCGAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCA…
mutable provenance: E.coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: lacZ promoter
sequence annotation: lacZa
sequence annotation: crRNA
sequence annotation: T7 promoter
sequence annotation: T7 terminator
sequence: https://synbiohub.org/public/igem/BBa_K1689000_sequence/1

lacZ promoter
type: SequenceAnnotation
persistent identity: annotation2471927
display id: annotation2471927
version: 1
top level: BBa_K1689000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1689000/annotation2471927/range2471927/1

https://synbiohub.org/public/igem/BBa_K1689000/annotation2471927/range2471927/1
type: Range
persistent identity: range2471927
display id: range2471927
version: 1
direction: direction/reverse
top level: BBa_K1689000
start: 303
end: 424
orientation: inline

lacZa
type: SequenceAnnotation
persistent identity: annotation2471926
display id: annotation2471926
version: 1
top level: BBa_K1689000
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1689000/annotation2471926/range2471926/1

https://synbiohub.org/public/igem/BBa_K1689000/annotation2471926/range2471926/1
type: Range
persistent identity: range2471926
display id: range2471926
version: 1
direction: direction/reverse
top level: BBa_K1689000
start: 27
end: 302
orientation: inline

crRNA
type: SequenceAnnotation
persistent identity: annotation2471928
display id: annotation2471928
version: 1
top level: BBa_K1689000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1689000/annotation2471928/range2471928/1

https://synbiohub.org/public/igem/BBa_K1689000/annotation2471928/range2471928/1
type: Range
persistent identity: range2471928
display id: range2471928
version: 1
direction: direction/forward
top level: BBa_K1689000
start: 432
end: 511
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2471925
display id: annotation2471925
version: 1
top level: BBa_K1689000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1689000/annotation2471925/range2471925/1

https://synbiohub.org/public/igem/BBa_K1689000/annotation2471925/range2471925/1
type: Range
persistent identity: range2471925
display id: range2471925
version: 1
direction: direction/forward
top level: BBa_K1689000
start: 1
end: 19
orientation: inline

T7 terminator
type: SequenceAnnotation
persistent identity: annotation2471929
display id: annotation2471929
version: 1
top level: BBa_K1689000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1689000/annotation2471929/range2471929/1

https://synbiohub.org/public/igem/BBa_K1689000/annotation2471929/range2471929/1
type: Range
persistent identity: range2471929
display id: range2471929
version: 1
direction: direction/forward
top level: BBa_K1689000
start: 512
end: 557
orientation: inline

BBa_K1614016
type: ComponentDefinition
persistent identity: BBa K1614016
display id: BBa_K1614016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Hammerhead Ribozyme and Malchite Green Aptamer in BBF RFC 110 transcription cassette
creator: Frieda Anna Sorgenfrei
created: 2015-09-15T11:00:00Z
modified: 2015-09-16T05:09:22Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2031_
m user id: 24664
owner id: 24664
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Hammerhead Ribozyme (HHR) and Malchite Green (MG) Aptamer in BBF RFC 110 transcription cassette for live tracking of in vitro transcription.
mutable notes: Cloned without restriction enzymes to avoid issues occurring from conserved cut sites. For more details see BBF RFC 110.
mutable provenance: Synthetic, Malchit Green aptamer (Grate, D., and Wilson, C. (1999). Laser-mediated, site-specific inactivation of RNA transcripts. Proceedings of the National Academy of Sciences of the United States of America 96, 6131-6136.) HHR (Prody, G.A., Bakos, J.T., Buzayan, J.M., Schneider, I.R., and Bruening, G. (1986). Autolytic processing of dimeric plant virus satellite RNA. Science 231, 1577-1580)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence annotation: G required for transcription
sequence annotation: T7 promotor
sequence annotation: HDV
sequence annotation: Malachite Green Aptamer
sequence annotation: HHR
sequence: https://synbiohub.org/public/igem/BBa_K1614016_sequence/1

G required for transcription
type: SequenceAnnotation
persistent identity: annotation2459467
display id: annotation2459467
version: 1
top level: BBa_K1614016
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1614016/annotation2459467/range2459467/1

https://synbiohub.org/public/igem/BBa_K1614016/annotation2459467/range2459467/1
type: Range
persistent identity: range2459467
display id: range2459467
version: 1
direction: direction/forward
top level: BBa_K1614016
start: 30
end: 30
orientation: inline

T7 promotor
type: SequenceAnnotation
persistent identity: annotation2459466
display id: annotation2459466
version: 1
top level: BBa_K1614016
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1614016/annotation2459466/range2459466/1

https://synbiohub.org/public/igem/BBa_K1614016/annotation2459466/range2459466/1
type: Range
persistent identity: range2459466
display id: range2459466
version: 1
direction: direction/forward
top level: BBa_K1614016
start: 13
end: 29
orientation: inline

HDV
type: SequenceAnnotation
persistent identity: annotation2459506
display id: annotation2459506
version: 1
top level: BBa_K1614016
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1614016/annotation2459506/range2459506/1

https://synbiohub.org/public/igem/BBa_K1614016/annotation2459506/range2459506/1
type: Range
persistent identity: range2459506
display id: range2459506
version: 1
direction: direction/forward
top level: BBa_K1614016
start: 123
end: 206
orientation: inline

Malachite Green Aptamer
type: SequenceAnnotation
persistent identity: annotation2459490
display id: annotation2459490
version: 1
top level: BBa_K1614016
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1614016/annotation2459490/range2459490/1

https://synbiohub.org/public/igem/BBa_K1614016/annotation2459490/range2459490/1
type: Range
persistent identity: range2459490
display id: range2459490
version: 1
direction: direction/forward
top level: BBa_K1614016
start: 85
end: 122
orientation: inline

HHR
type: SequenceAnnotation
persistent identity: annotation2459489
display id: annotation2459489
version: 1
top level: BBa_K1614016
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1614016/annotation2459489/range2459489/1

https://synbiohub.org/public/igem/BBa_K1614016/annotation2459489/range2459489/1
type: Range
persistent identity: range2459489
display id: range2459489
version: 1
direction: direction/forward
top level: BBa_K1614016
start: 31
end: 84
orientation: inline

BBa_K1763000
type: ComponentDefinition
persistent identity: BBa K1763000
display id: BBa_K1763000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Apis mellifera (honeybee) silk fibroin 3
creator: Daniel Cancilla
created: 2015-05-20T11:00:00Z
modified: 2015-05-21T07:56:16Z
discontinued: false
dominant: false
group u list: _2185_
m user id: 18035
owner id: 18035
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This is the coding region for the honeybee silk protein #3 (will update more later)
mutable notes: Will update more later (mention that signal peptide has been removed)
mutable provenance: Apis mellifera genome. Reference Genbank FJ235090.1
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: start codon
sequence annotation: Honey bee silk coding region
sequence: https://synbiohub.org/public/igem/BBa_K1763000_sequence/1

start codon
type: SequenceAnnotation
persistent identity: annotation2432105
display id: annotation2432105
version: 1
top level: BBa_K1763000
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1763000/annotation2432105/range2432105/1

https://synbiohub.org/public/igem/BBa_K1763000/annotation2432105/range2432105/1
type: Range
persistent identity: range2432105
display id: range2432105
version: 1
direction: direction/forward
top level: BBa_K1763000
start: 1
end: 3
orientation: inline

Honey bee silk coding region
type: SequenceAnnotation
persistent identity: annotation2432106
display id: annotation2432106
version: 1
top level: BBa_K1763000
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1763000/annotation2432106/range2432106/1

https://synbiohub.org/public/igem/BBa_K1763000/annotation2432106/range2432106/1
type: Range
persistent identity: range2432106
display id: range2432106
version: 1
direction: direction/forward
top level: BBa_K1763000
start: 1
end: 951
orientation: inline

BBa_K1632001
type: ComponentDefinition
persistent identity: BBa K1632001
display id: BBa_K1632001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: fim switch[default OFF](Tokyo_Tech/J23119)
creator: Riku Shinohara
created: 2015-08-12T11:00:00Z
modified: 2015-09-15T12:53:21Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _2049_
m user id: 24760
owner id: 22687
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: 1
mutable notes: 1
mutable provenance: 1
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Asc1
sequence annotation: Sal1
sequence annotation: BamH1
sequence annotation: Mlu1
sequence annotation: Bgl2
sequence annotation: J23119 promoter
sequence: https://synbiohub.org/public/igem/BBa_K1632001_sequence/1

Asc1
type: SequenceAnnotation
persistent identity: annotation2435272
display id: annotation2435272
version: 1
top level: BBa_K1632001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1632001/annotation2435272/range2435272/1

https://synbiohub.org/public/igem/BBa_K1632001/annotation2435272/range2435272/1
type: Range
persistent identity: range2435272
display id: range2435272
version: 1
direction: direction/reverse
top level: BBa_K1632001
start: 84
end: 91
orientation: inline

Sal1
type: SequenceAnnotation
persistent identity: annotation2435627
display id: annotation2435627
version: 1
top level: BBa_K1632001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1632001/annotation2435627/range2435627/1

https://synbiohub.org/public/igem/BBa_K1632001/annotation2435627/range2435627/1
type: Range
persistent identity: range2435627
display id: range2435627
version: 1
direction: direction/reverse
top level: BBa_K1632001
start: 147
end: 152
orientation: inline

BamH1
type: SequenceAnnotation
persistent identity: annotation2435625
display id: annotation2435625
version: 1
top level: BBa_K1632001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1632001/annotation2435625/range2435625/1

https://synbiohub.org/public/igem/BBa_K1632001/annotation2435625/range2435625/1
type: Range
persistent identity: range2435625
display id: range2435625
version: 1
direction: direction/reverse
top level: BBa_K1632001
start: 133
end: 138
orientation: inline

Asc1
type: SequenceAnnotation
persistent identity: annotation2435626
display id: annotation2435626
version: 1
top level: BBa_K1632001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1632001/annotation2435626/range2435626/1

https://synbiohub.org/public/igem/BBa_K1632001/annotation2435626/range2435626/1
type: Range
persistent identity: range2435626
display id: range2435626
version: 1
direction: direction/reverse
top level: BBa_K1632001
start: 139
end: 146
orientation: inline

Mlu1
type: SequenceAnnotation
persistent identity: annotation2435623
display id: annotation2435623
version: 1
top level: BBa_K1632001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1632001/annotation2435623/range2435623/1

https://synbiohub.org/public/igem/BBa_K1632001/annotation2435623/range2435623/1
type: Range
persistent identity: range2435623
display id: range2435623
version: 1
direction: direction/reverse
top level: BBa_K1632001
start: 78
end: 83
orientation: inline

Bgl2
type: SequenceAnnotation
persistent identity: annotation2435273
display id: annotation2435273
version: 1
top level: BBa_K1632001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1632001/annotation2435273/range2435273/1

https://synbiohub.org/public/igem/BBa_K1632001/annotation2435273/range2435273/1
type: Range
persistent identity: range2435273
display id: range2435273
version: 1
direction: direction/reverse
top level: BBa_K1632001
start: 92
end: 97
orientation: inline

J23119 promoter
type: SequenceAnnotation
persistent identity: annotation2435624
display id: annotation2435624
version: 1
top level: BBa_K1632001
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1632001/annotation2435624/range2435624/1

https://synbiohub.org/public/igem/BBa_K1632001/annotation2435624/range2435624/1
type: Range
persistent identity: range2435624
display id: range2435624
version: 1
direction: direction/reverse
top level: BBa_K1632001
start: 98
end: 132
orientation: inline

BBa_K1321100
type: ComponentDefinition
persistent identity: BBa K1321100
display id: BBa_K1321100
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Phytochelatin (PC) EC20 fused to linker-dCBD with T7 promoter
creator: Xenia Spencer-Milnes
created: 2014-10-08T11:00:00Z
modified: 2015-06-17T12:18:05Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 4206
owner id: 20780
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Phytochelatin EC(20) +CBD2 with T7. TBC
mutable notes: Phytochelatin EC(20) +CBD2 with T7. TBC
mutable provenance: Phytochelatin EC(20) +CBD2 with T7. TBC
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1319106
component: BBa_K1321340
component: BBa_B0105
component: BBa_K1321338
component: BBa_K1321005
sequence annotation: BBa_K1319106
sequence annotation: BBa_K1321340
sequence annotation: BBa_K1321338
sequence annotation: BBa_K1321005
sequence annotation: BBa_B0105
sequence: https://synbiohub.org/public/igem/BBa_K1321100_sequence/1

BBa_K1319106
type: Component
persistent identity: component2415249
display id: component2415249
version: 1
top level: BBa_K1321100
access: public
definition: https://synbiohub.org/public/igem/BBa_K1319106/1

BBa_K1321340
type: Component
persistent identity: component2415255
display id: component2415255
version: 1
top level: BBa_K1321100
access: public
definition: BBa_K1321340

BBa_B0105
type: Component
persistent identity: component2415253
display id: component2415253
version: 1
top level: BBa_K1321100
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_K1321338
type: Component
persistent identity: component2415247
display id: component2415247
version: 1
top level: BBa_K1321100
access: public
definition: BBa_K1321338

BBa_K1321005
type: Component
persistent identity: component2415251
display id: component2415251
version: 1
top level: BBa_K1321100
access: public
definition: BBa_K1321005

BBa_K1319106
type: SequenceAnnotation
persistent identity: annotation2415249
display id: annotation2415249
version: 1
top level: BBa_K1321100
location: https://synbiohub.org/public/igem/BBa_K1321100/annotation2415249/range2415249/1
component: BBa_K1319106

https://synbiohub.org/public/igem/BBa_K1321100/annotation2415249/range2415249/1
type: Range
persistent identity: range2415249
display id: range2415249
version: 1
direction: direction/reverse
top level: BBa_K1321100
start: 44
end: 58
orientation: inline

BBa_K1321340
type: SequenceAnnotation
persistent identity: annotation2415255
display id: annotation2415255
version: 1
top level: BBa_K1321100
location: https://synbiohub.org/public/igem/BBa_K1321100/annotation2415255/range2415255/1
component: BBa_K1321340

https://synbiohub.org/public/igem/BBa_K1321100/annotation2415255/range2415255/1
type: Range
persistent identity: range2415255
display id: range2415255
version: 1
direction: direction/reverse
top level: BBa_K1321100
start: 188
end: 553
orientation: inline

BBa_K1321338
type: SequenceAnnotation
persistent identity: annotation2415247
display id: annotation2415247
version: 1
top level: BBa_K1321100
location: https://synbiohub.org/public/igem/BBa_K1321100/annotation2415247/range2415247/1
component: BBa_K1321338

https://synbiohub.org/public/igem/BBa_K1321100/annotation2415247/range2415247/1
type: Range
persistent identity: range2415247
display id: range2415247
version: 1
direction: direction/reverse
top level: BBa_K1321100
start: 1
end: 43
orientation: inline

BBa_K1321005
type: SequenceAnnotation
persistent identity: annotation2415251
display id: annotation2415251
version: 1
top level: BBa_K1321100
location: https://synbiohub.org/public/igem/BBa_K1321100/annotation2415251/range2415251/1
component: BBa_K1321005

https://synbiohub.org/public/igem/BBa_K1321100/annotation2415251/range2415251/1
type: Range
persistent identity: range2415251
display id: range2415251
version: 1
direction: direction/reverse
top level: BBa_K1321100
start: 59
end: 181
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2415253
display id: annotation2415253
version: 1
top level: BBa_K1321100
location: https://synbiohub.org/public/igem/BBa_K1321100/annotation2415253/range2415253/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_K1321100/annotation2415253/range2415253/1
type: Range
persistent identity: range2415253
display id: range2415253
version: 1
direction: direction/reverse
top level: BBa_K1321100
start: 182
end: 187
orientation: inline

BBa_K1321090
type: ComponentDefinition
persistent identity: BBa K1321090
display id: BBa_K1321090
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Phytochelatin (PC) EC20 + Linker-dCBD
creator: Xenia Spencer-Milnes
created: 2014-10-06T11:00:00Z
modified: 2015-06-17T12:17:05Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 4206
owner id: 20780
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Phytochelatin EC(20) +CBD2. TBC
mutable notes: Codon optimised for E.coli. TBC
mutable provenance: CBD2 is EnLinker-CBH2-EnLinker-CBH1 from T.reesei. TBC
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1321005
component: BBa_K1321340
component: BBa_B0105
sequence annotation: BBa_B0105
sequence annotation: BBa_K1321005
sequence annotation: BBa_K1321340
sequence: https://synbiohub.org/public/igem/BBa_K1321090_sequence/1

BBa_K1321005
type: Component
persistent identity: component2414669
display id: component2414669
version: 1
top level: BBa_K1321090
access: public
definition: BBa_K1321005

BBa_K1321340
type: Component
persistent identity: component2414673
display id: component2414673
version: 1
top level: BBa_K1321090
access: public
definition: BBa_K1321340

BBa_B0105
type: Component
persistent identity: component2414671
display id: component2414671
version: 1
top level: BBa_K1321090
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2414671
display id: annotation2414671
version: 1
top level: BBa_K1321090
location: https://synbiohub.org/public/igem/BBa_K1321090/annotation2414671/range2414671/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_K1321090/annotation2414671/range2414671/1
type: Range
persistent identity: range2414671
display id: range2414671
version: 1
direction: direction/reverse
top level: BBa_K1321090
start: 124
end: 129
orientation: inline

BBa_K1321005
type: SequenceAnnotation
persistent identity: annotation2414669
display id: annotation2414669
version: 1
top level: BBa_K1321090
location: https://synbiohub.org/public/igem/BBa_K1321090/annotation2414669/range2414669/1
component: BBa_K1321005

https://synbiohub.org/public/igem/BBa_K1321090/annotation2414669/range2414669/1
type: Range
persistent identity: range2414669
display id: range2414669
version: 1
direction: direction/reverse
top level: BBa_K1321090
start: 1
end: 123
orientation: inline

BBa_K1321340
type: SequenceAnnotation
persistent identity: annotation2414673
display id: annotation2414673
version: 1
top level: BBa_K1321090
location: https://synbiohub.org/public/igem/BBa_K1321090/annotation2414673/range2414673/1
component: BBa_K1321340

https://synbiohub.org/public/igem/BBa_K1321090/annotation2414673/range2414673/1
type: Range
persistent identity: range2414673
display id: range2414673
version: 1
direction: direction/reverse
top level: BBa_K1321090
start: 130
end: 495
orientation: inline

BBa_K1758120
type: ComponentDefinition
persistent identity: BBa K1758120
display id: BBa_K1758120
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RNase E regulating protein RraA
creator: Team Bielefeld-CeBiTec 2015
created: 2015-08-12T11:00:00Z
modified: 2015-09-18T07:05:34Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2180_
m user id: 26563
owner id: 26563
owning group id: 9
status: status/Planning
bookmark: true
mutable description: The product of the RraA-gene has been reported to interact with RNase E from ''E. coli'' and to alternate its activity. Airen showed that if RraA-protein is added to a cell free protein synthesis reaction, the productivity raises about 30% (Airen, 2011). He postulated that activity of RNase E in the reaction is lowered due to the interaction with RraA-protein, therefore mRNA-levels are stabilized. RNase E is brought to the ''in vitro'' reaction by ''E. coli'' cell extract itself.
mutable notes: '''Amplification with Gibson-primer:''' *Amplification of regulator of ribonuclease activity A (''RraA''): '''Forward primer RraA''' 5'-ATGAAATACGATACTTCCGAGCTTTG-3' '''Reverse primer RraA''' 5'-TCATTCAATATCCAGCGGATCTTC-3' *Amplification of the pSB1C3-Backbone: '''Forward primer pSB1C3 RraA(43 bp)''' 5'-TCAGAAGATCCGCTGGATATTGAATGATACTAGTAGCGGCCGCTGCAG-3' '''Reverse primer pSB1C3 RraA(43 bp)''' 5'-TTCTTCCAGTTGTTCCGGGTTTGCCATCTCTAGAAGCGGCCGCGAATTCC-3' '''Ligation via [http://2015.igem.org/Team:Bielefeld-CeBiTec/Protocols Gibson-Assembly].'''
mutable provenance: RraA was obtained via PCR on ''E. coli'' K12 genomic DNA.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: rraA (menG)
sequence: https://synbiohub.org/public/igem/BBa_K1758120_sequence/1

rraA (menG)
type: SequenceAnnotation
persistent identity: annotation2435621
display id: annotation2435621
version: 1
top level: BBa_K1758120
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1758120/annotation2435621/range2435621/1

https://synbiohub.org/public/igem/BBa_K1758120/annotation2435621/range2435621/1
type: Range
persistent identity: range2435621
display id: range2435621
version: 1
direction: direction/forward
top level: BBa_K1758120
start: 1
end: 486
orientation: inline

BBa_K1602044
type: ComponentDefinition
persistent identity: BBa K1602044
display id: BBa_K1602044
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RRL3H
creator: Christian Sator,Stefan Zens, Max Zander, Benedikt Spannenkrebs, Sebastian Jaeger
created: 2015-09-15T11:00:00Z
modified: 2015-09-25T05:18:30Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _2019_
m user id: 23028
owner id: 26220
owning group id: 9
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J23100
component: BBa_J01080
component: BBa_K1497008
sequence annotation: BBa_J23100
sequence annotation: BBa_K1497008
sequence annotation: BBa_J01080
sequence: https://synbiohub.org/public/igem/BBa_K1602044_sequence/1

BBa_J23100
type: Component
persistent identity: component2462882
display id: component2462882
version: 1
top level: BBa_K1602044
access: public
definition: https://synbiohub.org/public/igem/BBa_J23100/1

BBa_J01080
type: Component
persistent identity: component2462883
display id: component2462883
version: 1
top level: BBa_K1602044
access: public
definition: https://synbiohub.org/public/igem/BBa_J01080/1

BBa_K1497008
type: Component
persistent identity: component2462884
display id: component2462884
version: 1
top level: BBa_K1602044
access: public
definition: BBa_K1497008

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2462882
display id: annotation2462882
version: 1
top level: BBa_K1602044
location: https://synbiohub.org/public/igem/BBa_K1602044/annotation2462882/range2462882/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K1602044/annotation2462882/range2462882/1
type: Range
persistent identity: range2462882
display id: range2462882
version: 1
direction: direction/reverse
top level: BBa_K1602044
start: 1
end: 35
orientation: inline

BBa_K1497008
type: SequenceAnnotation
persistent identity: annotation2462884
display id: annotation2462884
version: 1
top level: BBa_K1602044
location: https://synbiohub.org/public/igem/BBa_K1602044/annotation2462884/range2462884/1
component: BBa_K1497008

https://synbiohub.org/public/igem/BBa_K1602044/annotation2462884/range2462884/1
type: Range
persistent identity: range2462884
display id: range2462884
version: 1
direction: direction/reverse
top level: BBa_K1602044
start: 90
end: 245
orientation: inline

BBa_J01080
type: SequenceAnnotation
persistent identity: annotation2462883
display id: annotation2462883
version: 1
top level: BBa_K1602044
location: https://synbiohub.org/public/igem/BBa_K1602044/annotation2462883/range2462883/1
component: BBa_J01080

https://synbiohub.org/public/igem/BBa_K1602044/annotation2462883/range2462883/1
type: Range
persistent identity: range2462883
display id: range2462883
version: 1
direction: direction/reverse
top level: BBa_K1602044
start: 44
end: 83
orientation: inline

BBa_K1763427
type: ComponentDefinition
persistent identity: BBa K1763427
display id: BBa_K1763427
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: MaSp2-6(T7)
creator: Vinson Lam
created: 2015-09-14T11:00:00Z
modified: 2015-09-15T08:41:53Z
discontinued: false
dominant: false
group u list: _2185_
m user id: 27136
owner id: 27136
owning group id: 9
status: status/Planning
bookmark: false
mutable description: M2-6(1C3) under control of T7 promoter RBS.
mutable notes: no
mutable provenance: N. clavipes
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K1763426
component: BBa_K525998
sequence annotation: BBa_K525998
sequence annotation: BBa_K1763426
sequence: https://synbiohub.org/public/igem/BBa_K1763427_sequence/1

BBa_K1763426
type: Component
persistent identity: component2456838
display id: component2456838
version: 1
top level: BBa_K1763427
access: public
definition: BBa_K1763426

BBa_K525998
type: Component
persistent identity: component2456837
display id: component2456837
version: 1
top level: BBa_K1763427
access: public
definition: https://synbiohub.org/public/igem/BBa_K525998/1

BBa_K525998
type: SequenceAnnotation
persistent identity: annotation2456837
display id: annotation2456837
version: 1
top level: BBa_K1763427
location: https://synbiohub.org/public/igem/BBa_K1763427/annotation2456837/range2456837/1
component: BBa_K525998

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type: Range
persistent identity: range2456837
display id: range2456837
version: 1
direction: direction/reverse
top level: BBa_K1763427
start: 1
end: 32
orientation: inline

BBa_K1763426
type: SequenceAnnotation
persistent identity: annotation2456838
display id: annotation2456838
version: 1
top level: BBa_K1763427
location: https://synbiohub.org/public/igem/BBa_K1763427/annotation2456838/range2456838/1
component: BBa_K1763426

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type: Range
persistent identity: range2456838
display id: range2456838
version: 1
direction: direction/reverse
top level: BBa_K1763427
start: 39
end: 701
orientation: inline

BBa_K1602058
type: ComponentDefinition
persistent identity: BBa K1602058
display id: BBa_K1602058
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0034-yagE-B0034-yjhG-B0034-yqhD
creator: Leon Kraus, Jonathan Pletzer-Zelgert, Jonas Sindlinger, Carmen Sakas Gandullo, Sebastian Holzner, Elena Tonchevska, Mirjam Haun
created: 2015-09-15T11:00:00Z
modified: 2015-09-18T06:37:07Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2019_
m user id: 26220
owner id: 25990
owning group id: 9
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1602002
component: BBa_K1602013
component: BBa_K1602014
component: BBa_B0034
sequence annotation: BBa_K1602013
sequence annotation: BBa_K1602002
sequence annotation: BBa_B0034
sequence annotation: BBa_K1602014
sequence: https://synbiohub.org/public/igem/BBa_K1602058_sequence/1

BBa_K1602002
type: Component
persistent identity: component2463128
display id: component2463128
version: 1
top level: BBa_K1602058
access: public
definition: https://synbiohub.org/public/igem/BBa_K1602002/1

BBa_K1602013
type: Component
persistent identity: component2463131
display id: component2463131
version: 1
top level: BBa_K1602058
access: public
definition: BBa_K1602013

BBa_K1602014
type: Component
persistent identity: component2463124
display id: component2463124
version: 1
top level: BBa_K1602058
access: public
definition: BBa_K1602014

BBa_B0034
type: Component
persistent identity: component2463129
display id: component2463129
version: 1
top level: BBa_K1602058
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1602013
type: SequenceAnnotation
persistent identity: annotation2463131
display id: annotation2463131
version: 1
top level: BBa_K1602058
location: https://synbiohub.org/public/igem/BBa_K1602058/annotation2463131/range2463131/1
component: BBa_K1602013

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type: Range
persistent identity: range2463131
display id: range2463131
version: 1
direction: direction/reverse
top level: BBa_K1602058
start: 2948
end: 4111
orientation: inline

BBa_K1602002
type: SequenceAnnotation
persistent identity: annotation2463128
display id: annotation2463128
version: 1
top level: BBa_K1602058
location: https://synbiohub.org/public/igem/BBa_K1602058/annotation2463128/range2463128/1
component: BBa_K1602002

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type: Range
persistent identity: range2463128
display id: range2463128
version: 1
direction: direction/reverse
top level: BBa_K1602058
start: 936
end: 2921
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2463129
display id: annotation2463129
version: 1
top level: BBa_K1602058
location: https://synbiohub.org/public/igem/BBa_K1602058/annotation2463129/range2463129/1
component: BBa_B0034

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type: Range
persistent identity: range2463129
display id: range2463129
version: 1
direction: direction/reverse
top level: BBa_K1602058
start: 2930
end: 2941
orientation: inline

BBa_K1602014
type: SequenceAnnotation
persistent identity: annotation2463124
display id: annotation2463124
version: 1
top level: BBa_K1602058
location: https://synbiohub.org/public/igem/BBa_K1602058/annotation2463124/range2463124/1
component: BBa_K1602014

https://synbiohub.org/public/igem/BBa_K1602058/annotation2463124/range2463124/1
type: Range
persistent identity: range2463124
display id: range2463124
version: 1
direction: direction/reverse
top level: BBa_K1602058
start: 1
end: 927
orientation: inline

BBa_K1763001
type: ComponentDefinition
persistent identity: BBa K1763001
display id: BBa_K1763001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Apis mellifera (honeybee) silk fibroin 3 with regulatory elements
creator: Daniel Cancilla
created: 2015-05-20T11:00:00Z
modified: 2015-05-22T01:05:28Z
discontinued: false
dominant: false
group u list: _2185_
m user id: 18035
owner id: 18035
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Same as part BBa_K1763000 with regulatory elements upstream. These include lac promoter and rbs (will update later)
mutable notes: Will update later
mutable provenance: Apis mellifera genome. Reference Genbank FJ235090.1
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: BBa_R0010
sequence annotation: Honey bee silk coding region
sequence annotation: conserved
sequence annotation: start codon
sequence annotation: BBa_K1763000
sequence annotation: BBa_B0034
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_K1763001_sequence/1

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2432167
display id: annotation2432167
version: 1
top level: BBa_K1763001
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1763001/annotation2432167/range2432167/1

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type: Range
persistent identity: range2432167
display id: range2432167
version: 1
direction: direction/forward
top level: BBa_K1763001
start: 1
end: 200
orientation: inline

Honey bee silk coding region
type: SequenceAnnotation
persistent identity: annotation2432172
display id: annotation2432172
version: 1
top level: BBa_K1763001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1763001/annotation2432172/range2432172/1

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type: Range
persistent identity: range2432172
display id: range2432172
version: 1
direction: direction/forward
top level: BBa_K1763001
start: 213
end: 1163
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation2432170
display id: annotation2432170
version: 1
top level: BBa_K1763001
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K1763001/annotation2432170/range2432170/1

https://synbiohub.org/public/igem/BBa_K1763001/annotation2432170/range2432170/1
type: Range
persistent identity: range2432170
display id: range2432170
version: 1
direction: direction/forward
top level: BBa_K1763001
start: 205
end: 208
orientation: inline

start codon
type: SequenceAnnotation
persistent identity: annotation2432171
display id: annotation2432171
version: 1
top level: BBa_K1763001
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1763001/annotation2432171/range2432171/1

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type: Range
persistent identity: range2432171
display id: range2432171
version: 1
direction: direction/forward
top level: BBa_K1763001
start: 213
end: 215
orientation: inline

BBa_K1763000
type: SequenceAnnotation
persistent identity: annotation2432173
display id: annotation2432173
version: 1
top level: BBa_K1763001
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1763001/annotation2432173/range2432173/1

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type: Range
persistent identity: range2432173
display id: range2432173
version: 1
direction: direction/reverse
top level: BBa_K1763001
start: 213
end: 1163
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2432169
display id: annotation2432169
version: 1
top level: BBa_K1763001
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1763001/annotation2432169/range2432169/1

https://synbiohub.org/public/igem/BBa_K1763001/annotation2432169/range2432169/1
type: Range
persistent identity: range2432169
display id: range2432169
version: 1
direction: direction/reverse
top level: BBa_K1763001
start: 201
end: 212
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2432168
display id: annotation2432168
version: 1
top level: BBa_K1763001
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1763001/annotation2432168/range2432168/1

https://synbiohub.org/public/igem/BBa_K1763001/annotation2432168/range2432168/1
type: Range
persistent identity: range2432168
display id: range2432168
version: 1
direction: direction/forward
top level: BBa_K1763001
start: 173
end: 173
orientation: inline

BBa_K1758343
type: ComponentDefinition
persistent identity: BBa K1758343
display id: BBa_K1758343
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: MerR activator under constitutive promoter and induceble merT promoter with 5 UTR -sfGFP
creator: Team Bielefeld-CeBiTec 2015
created: 2015-09-07T11:00:00Z
modified: 2016-01-06T03:41:10Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2180_
m user id: 4206
owner id: 26630
owning group id: 9
status: status/Planning
bookmark: false
mutable description: ...
mutable notes: ...
mutable provenance: ...
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1758342
component: BBa_K1758340
sequence annotation: BBa_K1758340
sequence annotation: BBa_K1758342
sequence: https://synbiohub.org/public/igem/BBa_K1758343_sequence/1

BBa_K1758342
type: Component
persistent identity: component2447504
display id: component2447504
version: 1
top level: BBa_K1758343
access: public
definition: BBa_K1758342

BBa_K1758340
type: Component
persistent identity: component2447498
display id: component2447498
version: 1
top level: BBa_K1758343
access: public
definition: https://synbiohub.org/public/igem/BBa_K1758340/1

BBa_K1758340
type: SequenceAnnotation
persistent identity: annotation2447498
display id: annotation2447498
version: 1
top level: BBa_K1758343
location: https://synbiohub.org/public/igem/BBa_K1758343/annotation2447498/range2447498/1
component: BBa_K1758340

https://synbiohub.org/public/igem/BBa_K1758343/annotation2447498/range2447498/1
type: Range
persistent identity: range2447498
display id: range2447498
version: 1
direction: direction/reverse
top level: BBa_K1758343
start: 1
end: 496
orientation: inline

BBa_K1758342
type: SequenceAnnotation
persistent identity: annotation2447504
display id: annotation2447504
version: 1
top level: BBa_K1758343
location: https://synbiohub.org/public/igem/BBa_K1758343/annotation2447504/range2447504/1
component: BBa_K1758342

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type: Range
persistent identity: range2447504
display id: range2447504
version: 1
direction: direction/reverse
top level: BBa_K1758343
start: 505
end: 1470
orientation: inline

Intein
type: ComponentDefinition
persistent identity: BBa K1483003
display id: BBa_K1483003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ssp GyrB Split Intein
creator: Nikolas Layer, Philipp H. O. Mayer and Philip Roessler
created: 2014-10-05T11:00:00Z
modified: 2015-05-08T01:10:41Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1863_
m user id: 0
owner id: 21259
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: N-Intein part of the ssp GyrB split intein. Can be used in conjunction with the 6 amino acid long C-Intein to N-terminally immobilize a peptide onto a matrix. For immobilisation, parts need to be fused to the C-terminus of the N-intein. The N-terminus of the C-Intein is fused the matrix. The split intein recombines and splices itself out, thereby specificially immobilising the N-terminus of the peptide to the matrix. The sequence of the corresponding C-Intein is: H2N-GVPVHN-COOH (was aquired by peptide synthesis and is thus not part of the registry) This part can also be used in order to create fusion proteins, by attaching the C-Intein to a proein instead of the matrix. Part in RFC25 standard.
mutable notes: Part was designed in RFC25, since it is specifically used for fusion of peptides
mutable provenance: Intein of DNA grase subunit B of the cyanobacterium Synechocystis species, strain PCC6804. Sequence optimized for codon usage in E. coli K12 and synthesised.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Intein
sequence: https://synbiohub.org/public/igem/BBa_K1483003_sequence/1

Intein
type: SequenceAnnotation
persistent identity: annotation2396716
display id: annotation2396716
version: 1
top level: Intein
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1483003/annotation2396716/range2396716/1

https://synbiohub.org/public/igem/BBa_K1483003/annotation2396716/range2396716/1
type: Range
persistent identity: range2396716
display id: range2396716
version: 1
direction: direction/forward
top level: Intein
start: 1
end: 450
orientation: inline

BBa_K1365000
type: ComponentDefinition
persistent identity: BBa K1365000
display id: BBa_K1365000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NisA
creator: Sandra Mous
created: 2014-09-22T11:00:00Z
modified: 2015-05-08T01:10:07Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1741_
m user id: 0
owner id: 23000
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: NisA codes for the nisin precursor. Together with the genes NisB, NisT, NisC, NisP, NisR and NisK it is responsible for producing the lantibiotic nisin in Lactococcus lactis. Can be used in the Lactococcus lactis NZ9800, a strain with a deletion in the original NisA gene, to let it produce nisin.
mutable notes: -
mutable provenance: Obtained by PCR on the genome of L. lactis NZ9700, a nisin producing strain.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: start
sequence annotation: stop
sequence annotation: NisA
sequence: https://synbiohub.org/public/igem/BBa_K1365000_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation2385397
display id: annotation2385397
version: 1
top level: BBa_K1365000
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1365000/annotation2385397/range2385397/1

https://synbiohub.org/public/igem/BBa_K1365000/annotation2385397/range2385397/1
type: Range
persistent identity: range2385397
display id: range2385397
version: 1
direction: direction/forward
top level: BBa_K1365000
start: 1
end: 3
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2385398
display id: annotation2385398
version: 1
top level: BBa_K1365000
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1365000/annotation2385398/range2385398/1

https://synbiohub.org/public/igem/BBa_K1365000/annotation2385398/range2385398/1
type: Range
persistent identity: range2385398
display id: range2385398
version: 1
direction: direction/forward
top level: BBa_K1365000
start: 172
end: 174
orientation: inline

NisA
type: SequenceAnnotation
persistent identity: annotation2385399
display id: annotation2385399
version: 1
top level: BBa_K1365000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1365000/annotation2385399/range2385399/1

https://synbiohub.org/public/igem/BBa_K1365000/annotation2385399/range2385399/1
type: Range
persistent identity: range2385399
display id: range2385399
version: 1
direction: direction/forward
top level: BBa_K1365000
start: 1
end: 174
orientation: inline

BBa_K1362060
type: ComponentDefinition
persistent identity: BBa K1362060
display id: BBa_K1362060
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: OmpA surface protein as RFC[105] N-terminal insert
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B??scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch??fer, Carolin Schmelas, Silvan Schmitz, Max Waldha
created: 2014-10-07T11:00:00Z
modified: 2015-05-08T01:10:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1738_
m user id: 0
owner id: 22830
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
component: BBa_K1362425
component: BBa_K1362440
component: BBa_K1362460
component: BBa_K1362415
component: BBa_K103006
component: BBa_K1362423
sequence annotation: BBa_K1362460
sequence annotation: BBa_K1362415
sequence annotation: BBa_K1362440
sequence annotation: BBa_K103006
sequence annotation: BBa_K1362423
sequence annotation: BBa_K1362425
sequence: https://synbiohub.org/public/igem/BBa_K1362060_sequence/1

BBa_K1362425
type: Component
persistent identity: component2409749
display id: component2409749
version: 1
top level: BBa_K1362060
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362425/1

BBa_K1362440
type: Component
persistent identity: component2409750
display id: component2409750
version: 1
top level: BBa_K1362060
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362440/1

BBa_K1362460
type: Component
persistent identity: component2409757
display id: component2409757
version: 1
top level: BBa_K1362060
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362460/1

BBa_K1362415
type: Component
persistent identity: component2409758
display id: component2409758
version: 1
top level: BBa_K1362060
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362415/1

BBa_K103006
type: Component
persistent identity: component2409756
display id: component2409756
version: 1
top level: BBa_K1362060
access: public
definition: https://synbiohub.org/public/igem/BBa_K103006/1

BBa_K1362423
type: Component
persistent identity: component2409759
display id: component2409759
version: 1
top level: BBa_K1362060
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362423/1

BBa_K1362460
type: SequenceAnnotation
persistent identity: annotation2409757
display id: annotation2409757
version: 1
top level: BBa_K1362060
location: https://synbiohub.org/public/igem/BBa_K1362060/annotation2409757/range2409757/1
component: BBa_K1362460

https://synbiohub.org/public/igem/BBa_K1362060/annotation2409757/range2409757/1
type: Range
persistent identity: range2409757
display id: range2409757
version: 1
direction: direction/reverse
top level: BBa_K1362060
start: 476
end: 484
orientation: inline

BBa_K1362415
type: SequenceAnnotation
persistent identity: annotation2409758
display id: annotation2409758
version: 1
top level: BBa_K1362060
location: https://synbiohub.org/public/igem/BBa_K1362060/annotation2409758/range2409758/1
component: BBa_K1362415

https://synbiohub.org/public/igem/BBa_K1362060/annotation2409758/range2409758/1
type: Range
persistent identity: range2409758
display id: range2409758
version: 1
direction: direction/reverse
top level: BBa_K1362060
start: 485
end: 488
orientation: inline

BBa_K1362440
type: SequenceAnnotation
persistent identity: annotation2409750
display id: annotation2409750
version: 1
top level: BBa_K1362060
location: https://synbiohub.org/public/igem/BBa_K1362060/annotation2409750/range2409750/1
component: BBa_K1362440

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type: Range
persistent identity: range2409750
display id: range2409750
version: 1
direction: direction/reverse
top level: BBa_K1362060
start: 7
end: 10
orientation: inline

BBa_K103006
type: SequenceAnnotation
persistent identity: annotation2409756
display id: annotation2409756
version: 1
top level: BBa_K1362060
location: https://synbiohub.org/public/igem/BBa_K1362060/annotation2409756/range2409756/1
component: BBa_K103006

https://synbiohub.org/public/igem/BBa_K1362060/annotation2409756/range2409756/1
type: Range
persistent identity: range2409756
display id: range2409756
version: 1
direction: direction/reverse
top level: BBa_K1362060
start: 11
end: 474
orientation: inline

BBa_K1362423
type: SequenceAnnotation
persistent identity: annotation2409759
display id: annotation2409759
version: 1
top level: BBa_K1362060
location: https://synbiohub.org/public/igem/BBa_K1362060/annotation2409759/range2409759/1
component: BBa_K1362423

https://synbiohub.org/public/igem/BBa_K1362060/annotation2409759/range2409759/1
type: Range
persistent identity: range2409759
display id: range2409759
version: 1
direction: direction/reverse
top level: BBa_K1362060
start: 489
end: 495
orientation: inline

BBa_K1362425
type: SequenceAnnotation
persistent identity: annotation2409749
display id: annotation2409749
version: 1
top level: BBa_K1362060
location: https://synbiohub.org/public/igem/BBa_K1362060/annotation2409749/range2409749/1
component: BBa_K1362425

https://synbiohub.org/public/igem/BBa_K1362060/annotation2409749/range2409749/1
type: Range
persistent identity: range2409749
display id: range2409749
version: 1
direction: direction/reverse
top level: BBa_K1362060
start: 1
end: 6
orientation: inline

BBa_K1399004
type: ComponentDefinition
persistent identity: BBa K1399004
display id: BBa_K1399004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP (mut3b) with LVA-ssrA degradation tag
creator: Anna Stikane
created: 2014-09-18T11:00:00Z
modified: 2015-06-01T07:07:29Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1777_
m user id: 4206
owner id: 22477
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: GFP (mut3b) (see part BBa_E0040) with added LVA-ssrA degradation tag. The tag increases GFP turn-over rate, thus providing better temporal resolution of green fluorescence. In the same time, maximal fluorescence amplitudes will be lower as newly formed protein is degraded as soon as it is formed. The tag encodes peptide sequence AANDENYALVA and is recognized by ClpA and ClpX unfoldases and ClpX mediator SspB.[1] ClpA and ClpX then form a proteosome-like complex with ClpP protease and the protein is degraded.[1] The final three residues of the tag determines the strength of interaction with ClpX and thus the final protein degradation rate.[2] The LVA tag is reported to lead to fast protein degradation, degrading GFP with rate -0.018 per minute.[2] However, be aware that exact protein degradation rate depends on multiple factors: ClpXP and ClpAP protease and SspB mediator concentrations, protein stability, Km of binding to the protease, temperature [3]. References [1] Flynn, J. M. et al. Overlapping recognition determinants within the ssrA degradation tag allow modulation of proteolysis. Proc. Natl. Acad. Sci. U. S. A. 98, 10584???9 (2001). [2] Andersen, J. B. et al. New unstable variants of green fluorescent protein for studies of transient gene expression in bacteria. Appl. Environ. Microbiol. 64, 2240???6 (1998). [3] Purcell, O., Grierson, C. S., Bernardo, M. Di & Savery, N. J. Temperature dependence of ssrA-tag mediated protein degradation. J. Biol. Eng. 6, 10 (2012).
mutable notes: The tag was attached to GFP using PCR and MABEL (mutagenesis with blunt-end ligation), thus avoiding introduction of additonal residues and restriction site. Different parts of the tag are recognized by different proteins, for example, the final 3 residues (LVA in this case) are recognised by ClpX, whereas first 4 residues of the tag are required for efficient SspB binding.[1] Thus modifications of these critical residues alter the efficacy with what different proteases bind to it.
mutable provenance: GFP comes from part E1010, the tag sequence was obtained from paper by Andersen et al., (1998).[2]
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: stop
sequence annotation: start
sequence annotation: GFP (mut3b)
sequence annotation: LVA
sequence: https://synbiohub.org/public/igem/BBa_K1399004_sequence/1

stop
type: SequenceAnnotation
persistent identity: annotation2383916
display id: annotation2383916
version: 1
top level: BBa_K1399004
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399004/annotation2383916/range2383916/1

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type: Range
persistent identity: range2383916
display id: range2383916
version: 1
direction: direction/forward
top level: BBa_K1399004
start: 748
end: 750
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2383913
display id: annotation2383913
version: 1
top level: BBa_K1399004
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1399004/annotation2383913/range2383913/1

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type: Range
persistent identity: range2383913
display id: range2383913
version: 1
direction: direction/forward
top level: BBa_K1399004
start: 1
end: 3
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2383917
display id: annotation2383917
version: 1
top level: BBa_K1399004
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399004/annotation2383917/range2383917/1

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type: Range
persistent identity: range2383917
display id: range2383917
version: 1
direction: direction/forward
top level: BBa_K1399004
start: 751
end: 753
orientation: inline

GFP (mut3b)
type: SequenceAnnotation
persistent identity: annotation2383914
display id: annotation2383914
version: 1
top level: BBa_K1399004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1399004/annotation2383914/range2383914/1

https://synbiohub.org/public/igem/BBa_K1399004/annotation2383914/range2383914/1
type: Range
persistent identity: range2383914
display id: range2383914
version: 1
direction: direction/forward
top level: BBa_K1399004
start: 4
end: 714
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation2383915
display id: annotation2383915
version: 1
top level: BBa_K1399004
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1399004/annotation2383915/range2383915/1

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type: Range
persistent identity: range2383915
display id: range2383915
version: 1
direction: direction/forward
top level: BBa_K1399004
start: 715
end: 747
orientation: inline

Fla-Art175
type: ComponentDefinition
persistent identity: BBa K1659001
display id: BBa_K1659001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Artilysin Art-175 fused at N-terminal with flagellin 26-47 peptide segment
creator: Raphaella Hull
created: 2015-08-09T11:00:00Z
modified: 2016-02-01T12:51:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2077_
m user id: 4206
owner id: 24525
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Artilysins are an exciting class of enzyme-based antibacterials. Their name is derived from "artificial endolysin" and they exploit the lytic power of bacteriophage-encoded endolyins. Endolysins are peptidoglycan hydrolases produced at the end of the lytic cycle that pass through the cytoplasmic membrane, degrade the peptidoglycan layer and cause the osmotic lysis of the infected bacterial cell, thus liberating the progeny. Purified endolysins have been used to kill Gram-positive pathogens. Gram-negative bacteria, however, have a protective outer membrane containing lipopolysaccharide (LPS) that serves as a barrier against the peptidoglycan hydolytic activity of endolysins from the outside. To overcome this problem, selected polycationic or amphipathic peptides that locally destabilize the LPS layer have been covalently fused to endolysins. Biers et al. fused the sheep myeloid antimicrobial peptide (SMAP-29) to the N terminus of the endolysin KZ144 to create Artilysin 175. Art-175 has been shown to be a highly potent antibacterial that acts in minutes to kill virtually all Pseudomonas aeruginosa strains. [1] BBa_K1659001 is a composite of Art-175 with the 26-47 segment of Salmonella typhimurium flagellin combined with a hexahistidine tag. [2] Fla 26-47 is capable of mediating flagellar export of recombinant proteins and, combined with a hexahistidine tag, facilitates their secreted expression and easy purification. [1] Briers, Y., Walmagh, M., Grymonprez, B., Biebl, M., Pirnay, J. P., Defraine, V., ??? Lavigne, R. (2014). Art-175 is a highly efficient antibacterial against multidrug-resistant strains and persisters of Pseudomonas aeruginosa. Antimicrobial Agents and Chemotherapy, 58(7), 3774???3784. http://doi.org/10.1128/AAC.02668-14 [2] Balb??s, P., & Lorence, A. (2004). Recombinant Gene Expression. Reviews and Protocols, 267, 506. http://doi.org/10.1007/978-1-61779-433-9
mutable notes: Export signal combined with hexahistidine tag
mutable provenance: Art-175 amino acid sequence: Briers, Y., Walmagh, M., Grymonprez, B., Biebl, M., Pirnay, J. P., Defraine, V., ??? Lavigne, R. (2014). Art-175 is a highly efficient antibacterial against multidrug-resistant strains and persisters of Pseudomonas aeruginosa. Antimicrobial Agents and Chemotherapy, 58(7), 3774???3784. http://doi.org/10.1128/AAC.02668-14 Amino acid sequence of Fla 26-47: Balb??s, P., & Lorence, A. (2004). Recombinant Gene Expression. Reviews and Protocols, 267, 506. http://doi.org/10.1007/978-1-61779-433-9
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: stop
sequence annotation: Art-175
sequence annotation: start
sequence annotation: Flagellin 26-47 Secretion Signal Tag
sequence annotation: Hisx6
sequence: https://synbiohub.org/public/igem/BBa_K1659001_sequence/1

stop
type: SequenceAnnotation
persistent identity: annotation2438074
display id: annotation2438074
version: 1
top level: Fla-Art175
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1659001/annotation2438074/range2438074/1

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type: Range
persistent identity: range2438074
display id: range2438074
version: 1
direction: direction/forward
top level: Fla-Art175
start: 1054
end: 1056
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2438072
display id: annotation2438072
version: 1
top level: Fla-Art175
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1659001/annotation2438072/range2438072/1

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type: Range
persistent identity: range2438072
display id: range2438072
version: 1
direction: direction/forward
top level: Fla-Art175
start: 1051
end: 1053
orientation: inline

Art-175
type: SequenceAnnotation
persistent identity: annotation2438068
display id: annotation2438068
version: 1
top level: Fla-Art175
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1659001/annotation2438068/range2438068/1

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type: Range
persistent identity: range2438068
display id: range2438068
version: 1
direction: direction/forward
top level: Fla-Art175
start: 166
end: 1032
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2438066
display id: annotation2438066
version: 1
top level: Fla-Art175
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1659001/annotation2438066/range2438066/1

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type: Range
persistent identity: range2438066
display id: range2438066
version: 1
direction: direction/forward
top level: Fla-Art175
start: 1
end: 3
orientation: inline

Flagellin 26-47 Secretion Signal Tag
type: SequenceAnnotation
persistent identity: annotation2438058
display id: annotation2438058
version: 1
top level: Fla-Art175
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1659001/annotation2438058/range2438058/1

https://synbiohub.org/public/igem/BBa_K1659001/annotation2438058/range2438058/1
type: Range
persistent identity: range2438058
display id: range2438058
version: 1
direction: direction/forward
top level: Fla-Art175
start: 4
end: 165
orientation: inline

Hisx6
type: SequenceAnnotation
persistent identity: annotation2438070
display id: annotation2438070
version: 1
top level: Fla-Art175
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1659001/annotation2438070/range2438070/1

https://synbiohub.org/public/igem/BBa_K1659001/annotation2438070/range2438070/1
type: Range
persistent identity: range2438070
display id: range2438070
version: 1
direction: direction/forward
top level: Fla-Art175
start: 1033
end: 1050
orientation: inline

mRdRP
type: ComponentDefinition
persistent identity: BBa K1442101
display id: BBa_K1442101
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RdRP Mutant
creator: Dan Goss
created: 2014-10-05T11:00:00Z
modified: 2015-05-08T01:10:26Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1820_
m user id: 0
owner id: 23055
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: To test the RdRP against a control, we used a deliberately mutated version of itself. To mutate it, we used the restriction enzyme BgLII (code: agatct). This was not thought through but arbitrarily chosen, and resulted in a shift of frame and the introduction of a stop codon (tga).
mutable notes: Principally, our goal with this part was to create a non-functioning version of our RdRP which we could test it against, to ensure it was the RdRP proper that was having the desired effect of replicating our RNA.
mutable provenance: The source from which this mutant is derived is our RdRP proper, part BBa_K1442100.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Stop Codon TGA
sequence annotation: Untranslated Region
sequence: https://synbiohub.org/public/igem/BBa_K1442101_sequence/1

Stop Codon TGA
type: SequenceAnnotation
persistent identity: annotation2396851
display id: annotation2396851
version: 1
top level: mRdRP
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1442101/annotation2396851/range2396851/1

https://synbiohub.org/public/igem/BBa_K1442101/annotation2396851/range2396851/1
type: Range
persistent identity: range2396851
display id: range2396851
version: 1
direction: direction/forward
top level: mRdRP
start: 517
end: 519
orientation: inline

Untranslated Region
type: SequenceAnnotation
persistent identity: annotation2396853
display id: annotation2396853
version: 1
top level: mRdRP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1442101/annotation2396853/range2396853/1

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type: Range
persistent identity: range2396853
display id: range2396853
version: 1
direction: direction/forward
top level: mRdRP
start: 517
end: 868
orientation: inline

eYFP_SH3 g
type: ComponentDefinition
persistent identity: BBa K1602041
display id: BBa_K1602041
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Inducible generator of eYFP with C-terminal SH3 ligand
creator: Jasmin Weber, Michelle Zoeller, Marcus Geissler, Stefan Bohn, Simon Linder, Sebastian Barthel
created: 2015-09-16T11:00:00Z
modified: 2015-09-17T05:30:40Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2019_
m user id: 22022
owner id: 22022
owning group id: 9
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_I719005
component: BBa_K1602038
sequence annotation: BBa_K1602038
sequence annotation: BBa_I719005
sequence: https://synbiohub.org/public/igem/BBa_K1602041_sequence/1

BBa_I719005
type: Component
persistent identity: component2467302
display id: component2467302
version: 1
top level: eYFP_SH3 g
access: public
definition: https://synbiohub.org/public/igem/BBa_I719005/1

BBa_K1602038
type: Component
persistent identity: component2467303
display id: component2467303
version: 1
top level: eYFP_SH3 g
access: public
definition: eYFP_SH3li

BBa_K1602038
type: SequenceAnnotation
persistent identity: annotation2467303
display id: annotation2467303
version: 1
top level: eYFP_SH3 g
location: https://synbiohub.org/public/igem/BBa_K1602041/annotation2467303/range2467303/1
component: BBa_K1602038

https://synbiohub.org/public/igem/BBa_K1602041/annotation2467303/range2467303/1
type: Range
persistent identity: range2467303
display id: range2467303
version: 1
direction: direction/reverse
top level: eYFP_SH3 g
start: 32
end: 817
orientation: inline

BBa_I719005
type: SequenceAnnotation
persistent identity: annotation2467302
display id: annotation2467302
version: 1
top level: eYFP_SH3 g
location: https://synbiohub.org/public/igem/BBa_K1602041/annotation2467302/range2467302/1
component: BBa_I719005

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type: Range
persistent identity: range2467302
display id: range2467302
version: 1
direction: direction/reverse
top level: eYFP_SH3 g
start: 1
end: 23
orientation: inline

1Gate-NpuD
type: ComponentDefinition
persistent identity: BBa K1362100
display id: BBa_K1362100
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS + NpuDnaE N-intein RFC[105] assembly construct (with His6)
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Buescher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schaefer, Carolin Schmelas, Silvan Schmitz, Max Waldhauer
created: 2014-10-04T11:00:00Z
modified: 2015-05-08T01:10:04Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1738_
m user id: 0
owner id: 22830
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: ???
mutable notes: ???
mutable provenance: ???
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1362421
component: BBa_K1362400
component: BBa_K1362414
component: BBa_K1362417
component: BBa_K1362437
component: BBa_K1362090
component: BBa_G0000
component: BBa_K1362430
component: BBa_K1362422
component: BBa_K1362415
sequence annotation: BBa_G0000
sequence annotation: BBa_K1362417
sequence annotation: BBa_K1362421
sequence annotation: BBa_K1362422
sequence annotation: BBa_K1362414
sequence annotation: BBa_K1362090
sequence annotation: BBa_K1362437
sequence annotation: BBa_K1362430
sequence annotation: BBa_K1362415
sequence annotation: BBa_K1362400
sequence: https://synbiohub.org/public/igem/BBa_K1362100_sequence/1

BBa_K1362421
type: Component
persistent identity: component2410053
display id: component2410053
version: 1
top level: 1Gate-NpuD
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362421/1

BBa_K1362400
type: Component
persistent identity: component2410045
display id: component2410045
version: 1
top level: 1Gate-NpuD
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362400/1

BBa_K1362414
type: Component
persistent identity: component2410022
display id: component2410022
version: 1
top level: 1Gate-NpuD
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362414/1

BBa_K1362417
type: Component
persistent identity: component2410048
display id: component2410048
version: 1
top level: 1Gate-NpuD
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362417/1

BBa_K1362437
type: Component
persistent identity: component2410055
display id: component2410055
version: 1
top level: 1Gate-NpuD
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362437/1

BBa_K1362090
type: Component
persistent identity: component2410020
display id: component2410020
version: 1
top level: 1Gate-NpuD
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362090/1

BBa_G0000
type: Component
persistent identity: component2410021
display id: component2410021
version: 1
top level: 1Gate-NpuD
access: public
definition: https://synbiohub.org/public/igem/BBa_G0000/1

BBa_K1362430
type: Component
persistent identity: component2410051
display id: component2410051
version: 1
top level: 1Gate-NpuD
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362430/1

BBa_K1362422
type: Component
persistent identity: component2410043
display id: component2410043
version: 1
top level: 1Gate-NpuD
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362422/1

BBa_K1362415
type: Component
persistent identity: component2410044
display id: component2410044
version: 1
top level: 1Gate-NpuD
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362415/1

BBa_G0000
type: SequenceAnnotation
persistent identity: annotation2410021
display id: annotation2410021
version: 1
top level: 1Gate-NpuD
location: https://synbiohub.org/public/igem/BBa_K1362100/annotation2410021/range2410021/1
component: BBa_G0000

https://synbiohub.org/public/igem/BBa_K1362100/annotation2410021/range2410021/1
type: Range
persistent identity: range2410021
display id: range2410021
version: 1
direction: direction/reverse
top level: 1Gate-NpuD
start: 30
end: 35
orientation: inline

BBa_K1362417
type: SequenceAnnotation
persistent identity: annotation2410048
display id: annotation2410048
version: 1
top level: 1Gate-NpuD
location: https://synbiohub.org/public/igem/BBa_K1362100/annotation2410048/range2410048/1
component: BBa_K1362417

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type: Range
persistent identity: range2410048
display id: range2410048
version: 1
direction: direction/reverse
top level: 1Gate-NpuD
start: 1428
end: 1433
orientation: inline

BBa_K1362421
type: SequenceAnnotation
persistent identity: annotation2410053
display id: annotation2410053
version: 1
top level: 1Gate-NpuD
location: https://synbiohub.org/public/igem/BBa_K1362100/annotation2410053/range2410053/1
component: BBa_K1362421

https://synbiohub.org/public/igem/BBa_K1362100/annotation2410053/range2410053/1
type: Range
persistent identity: range2410053
display id: range2410053
version: 1
direction: direction/reverse
top level: 1Gate-NpuD
start: 1458
end: 1461
orientation: inline

BBa_K1362422
type: SequenceAnnotation
persistent identity: annotation2410043
display id: annotation2410043
version: 1
top level: 1Gate-NpuD
location: https://synbiohub.org/public/igem/BBa_K1362100/annotation2410043/range2410043/1
component: BBa_K1362422

https://synbiohub.org/public/igem/BBa_K1362100/annotation2410043/range2410043/1
type: Range
persistent identity: range2410043
display id: range2410043
version: 1
direction: direction/reverse
top level: 1Gate-NpuD
start: 39
end: 1121
orientation: inline

BBa_K1362414
type: SequenceAnnotation
persistent identity: annotation2410022
display id: annotation2410022
version: 1
top level: 1Gate-NpuD
location: https://synbiohub.org/public/igem/BBa_K1362100/annotation2410022/range2410022/1
component: BBa_K1362414

https://synbiohub.org/public/igem/BBa_K1362100/annotation2410022/range2410022/1
type: Range
persistent identity: range2410022
display id: range2410022
version: 1
direction: direction/reverse
top level: 1Gate-NpuD
start: 36
end: 38
orientation: inline

BBa_K1362090
type: SequenceAnnotation
persistent identity: annotation2410020
display id: annotation2410020
version: 1
top level: 1Gate-NpuD
location: https://synbiohub.org/public/igem/BBa_K1362100/annotation2410020/range2410020/1
component: BBa_K1362090

https://synbiohub.org/public/igem/BBa_K1362100/annotation2410020/range2410020/1
type: Range
persistent identity: range2410020
display id: range2410020
version: 1
direction: direction/reverse
top level: 1Gate-NpuD
start: 1
end: 29
orientation: inline

BBa_K1362437
type: SequenceAnnotation
persistent identity: annotation2410055
display id: annotation2410055
version: 1
top level: 1Gate-NpuD
location: https://synbiohub.org/public/igem/BBa_K1362100/annotation2410055/range2410055/1
component: BBa_K1362437

https://synbiohub.org/public/igem/BBa_K1362100/annotation2410055/range2410055/1
type: Range
persistent identity: range2410055
display id: range2410055
version: 1
direction: direction/reverse
top level: 1Gate-NpuD
start: 1462
end: 1464
orientation: inline

BBa_K1362430
type: SequenceAnnotation
persistent identity: annotation2410051
display id: annotation2410051
version: 1
top level: 1Gate-NpuD
location: https://synbiohub.org/public/igem/BBa_K1362100/annotation2410051/range2410051/1
component: BBa_K1362430

https://synbiohub.org/public/igem/BBa_K1362100/annotation2410051/range2410051/1
type: Range
persistent identity: range2410051
display id: range2410051
version: 1
direction: direction/reverse
top level: 1Gate-NpuD
start: 1434
end: 1457
orientation: inline

BBa_K1362415
type: SequenceAnnotation
persistent identity: annotation2410044
display id: annotation2410044
version: 1
top level: 1Gate-NpuD
location: https://synbiohub.org/public/igem/BBa_K1362100/annotation2410044/range2410044/1
component: BBa_K1362415

https://synbiohub.org/public/igem/BBa_K1362100/annotation2410044/range2410044/1
type: Range
persistent identity: range2410044
display id: range2410044
version: 1
direction: direction/reverse
top level: 1Gate-NpuD
start: 1122
end: 1125
orientation: inline

BBa_K1362400
type: SequenceAnnotation
persistent identity: annotation2410045
display id: annotation2410045
version: 1
top level: 1Gate-NpuD
location: https://synbiohub.org/public/igem/BBa_K1362100/annotation2410045/range2410045/1
component: BBa_K1362400

https://synbiohub.org/public/igem/BBa_K1362100/annotation2410045/range2410045/1
type: Range
persistent identity: range2410045
display id: range2410045
version: 1
direction: direction/reverse
top level: 1Gate-NpuD
start: 1126
end: 1427
orientation: inline

1GateNpuE
type: ComponentDefinition
persistent identity: BBa K1362101
display id: BBa_K1362101
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS+NpuDnaE C-intein RFC[105] assembly construct (with His6)
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Buescher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schaefer, Carolin Schmelas, Silvan Schmitz, Max Waldhauer
created: 2014-10-07T11:00:00Z
modified: 2015-05-08T01:10:04Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1738_
m user id: 0
owner id: 12377
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: This intein assembly construct is part of the iGEM team Heidelberg 2014's strategy for cloning with split inteins. Inteins are naturally occuring peptide sequences that splice out of a precursor protein and attach the remaining ends together to form a new protein. When splitting those intein sequence into an N-terminal and a C-terminal split intein one is left with a powerful tool to post-translationally modify whole proteins on the amino-acid sequence level. This construct was designed to express any protein of interest fused to the Nostoc punctiforme DnaE N-terminal split intein. The corresponding C-terminal construct is BBa_K1362101 . Upon coexpression or mixture of the N- and C-constructs protein splicing takes place and the N- and C-terminal proteins of interest are irreversibly assembled via a newly formed peptide bond. This mechanism can be applied for a variety of different uses such as the activation of a protein through reconstitution of individually expressed split halves. See our [http://2014.igem.org/Team:Heidelberg split sfGFP experiment] and the respective parts in the registry for more information. Protein splicing offers many new possibilities and we hope to have set a foundation that you guys can build on! ===Nostoc punctiforme DnaE split intein (NpuDnaE)=== Inteins can be found in all kingdoms of life, however their use for the organism remains yet unknown. In the past years many intein sequences were found in the genomes of cyanobacteria. NpuDnaE is a naturally split intein from the alpha subunit of Dna Polymerase III in Nostoc punctiforme bacteria which shows [[#References|[2]]] extraordinarly high splicing activity and therefore is on of the gold-standards for inteins since many years. Amino Acid Sequence: N-Intein: AEY | CLSYETEILTVEYGLLPIGKIVEKRIECTVYSVDNNGNIYTQPVAQWHDRGEQEVFEYCLEDGSLIRATKDHKFMTVDGQMLPIDEIFERELDLMRVDNLPN C-Intein: MIKIATRKYLGKQNVYDIGVERDHNFALKNGFIASN | CFN
mutable notes: ???
mutable provenance: The part is assembled from different sources. The intein sequences come addgene or were kindly provided by different working groups. BBa_J04450 was used as mRFP selection marker. The scars and overhangs are part of the RFC[???] intein cloning strategy and are further explained on our [http://2014.igem.org/Team:Heidelberg/parts/rfc wiki] and in RFC[???] [[#Reference|[1]]]. Please see the corresponding basic parts for the individual detailed information.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1362414
component: BBa_K1362464
component: BBa_K1362465
component: BBa_G0000
component: BBa_K1362090
component: BBa_K1362401
component: BBa_K1362419
component: BBa_K1362422
component: BBa_K1362421
component: BBa_K1362431
component: BBa_K1362418
sequence annotation: BBa_K1362401
sequence annotation: BBa_K1362419
sequence annotation: BBa_K1362414
sequence annotation: BBa_K1362464
sequence annotation: BBa_K1362465
sequence annotation: BBa_K1362431
sequence annotation: BBa_K1362421
sequence annotation: BBa_K1362418
sequence annotation: BBa_G0000
sequence annotation: BBa_K1362422
sequence annotation: BBa_K1362090
sequence: https://synbiohub.org/public/igem/BBa_K1362101_sequence/1

BBa_K1362414
type: Component
persistent identity: component2410060
display id: component2410060
version: 1
top level: 1GateNpuE
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362414/1

BBa_K1362464
type: Component
persistent identity: component2410066
display id: component2410066
version: 1
top level: 1GateNpuE
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362464/1

BBa_K1362465
type: Component
persistent identity: component2410092
display id: component2410092
version: 1
top level: 1GateNpuE
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362465/1

BBa_G0000
type: Component
persistent identity: component2410059
display id: component2410059
version: 1
top level: 1GateNpuE
access: public
definition: https://synbiohub.org/public/igem/BBa_G0000/1

BBa_K1362090
type: Component
persistent identity: component2410058
display id: component2410058
version: 1
top level: 1GateNpuE
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362090/1

BBa_K1362401
type: Component
persistent identity: component2410067
display id: component2410067
version: 1
top level: 1GateNpuE
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362401/1

BBa_K1362419
type: Component
persistent identity: component2410068
display id: component2410068
version: 1
top level: 1GateNpuE
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362419/1

BBa_K1362422
type: Component
persistent identity: component2410089
display id: component2410089
version: 1
top level: 1GateNpuE
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362422/1

BBa_K1362421
type: Component
persistent identity: component2410091
display id: component2410091
version: 1
top level: 1GateNpuE
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362421/1

BBa_K1362431
type: Component
persistent identity: component2410063
display id: component2410063
version: 1
top level: 1GateNpuE
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362431/1

BBa_K1362418
type: Component
persistent identity: component2410064
display id: component2410064
version: 1
top level: 1GateNpuE
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362418/1

BBa_K1362401
type: SequenceAnnotation
persistent identity: annotation2410067
display id: annotation2410067
version: 1
top level: 1GateNpuE
location: https://synbiohub.org/public/igem/BBa_K1362101/annotation2410067/range2410067/1
component: BBa_K1362401

https://synbiohub.org/public/igem/BBa_K1362101/annotation2410067/range2410067/1
type: Range
persistent identity: range2410067
display id: range2410067
version: 1
direction: direction/reverse
top level: 1GateNpuE
start: 66
end: 166
orientation: inline

BBa_K1362419
type: SequenceAnnotation
persistent identity: annotation2410068
display id: annotation2410068
version: 1
top level: 1GateNpuE
location: https://synbiohub.org/public/igem/BBa_K1362101/annotation2410068/range2410068/1
component: BBa_K1362419

https://synbiohub.org/public/igem/BBa_K1362101/annotation2410068/range2410068/1
type: Range
persistent identity: range2410068
display id: range2410068
version: 1
direction: direction/reverse
top level: 1GateNpuE
start: 167
end: 170
orientation: inline

BBa_K1362414
type: SequenceAnnotation
persistent identity: annotation2410060
display id: annotation2410060
version: 1
top level: 1GateNpuE
location: https://synbiohub.org/public/igem/BBa_K1362101/annotation2410060/range2410060/1
component: BBa_K1362414

https://synbiohub.org/public/igem/BBa_K1362101/annotation2410060/range2410060/1
type: Range
persistent identity: range2410060
display id: range2410060
version: 1
direction: direction/reverse
top level: 1GateNpuE
start: 36
end: 38
orientation: inline

BBa_K1362464
type: SequenceAnnotation
persistent identity: annotation2410066
display id: annotation2410066
version: 1
top level: 1GateNpuE
location: https://synbiohub.org/public/igem/BBa_K1362101/annotation2410066/range2410066/1
component: BBa_K1362464

https://synbiohub.org/public/igem/BBa_K1362101/annotation2410066/range2410066/1
type: Range
persistent identity: range2410066
display id: range2410066
version: 1
direction: direction/reverse
top level: 1GateNpuE
start: 64
end: 65
orientation: inline

BBa_K1362465
type: SequenceAnnotation
persistent identity: annotation2410092
display id: annotation2410092
version: 1
top level: 1GateNpuE
location: https://synbiohub.org/public/igem/BBa_K1362101/annotation2410092/range2410092/1
component: BBa_K1362465

https://synbiohub.org/public/igem/BBa_K1362101/annotation2410092/range2410092/1
type: Range
persistent identity: range2410092
display id: range2410092
version: 1
direction: direction/reverse
top level: 1GateNpuE
start: 1258
end: 1259
orientation: inline

BBa_K1362431
type: SequenceAnnotation
persistent identity: annotation2410063
display id: annotation2410063
version: 1
top level: 1GateNpuE
location: https://synbiohub.org/public/igem/BBa_K1362101/annotation2410063/range2410063/1
component: BBa_K1362431

https://synbiohub.org/public/igem/BBa_K1362101/annotation2410063/range2410063/1
type: Range
persistent identity: range2410063
display id: range2410063
version: 1
direction: direction/reverse
top level: 1GateNpuE
start: 39
end: 59
orientation: inline

BBa_K1362421
type: SequenceAnnotation
persistent identity: annotation2410091
display id: annotation2410091
version: 1
top level: 1GateNpuE
location: https://synbiohub.org/public/igem/BBa_K1362101/annotation2410091/range2410091/1
component: BBa_K1362421

https://synbiohub.org/public/igem/BBa_K1362101/annotation2410091/range2410091/1
type: Range
persistent identity: range2410091
display id: range2410091
version: 1
direction: direction/reverse
top level: 1GateNpuE
start: 1254
end: 1257
orientation: inline

BBa_K1362418
type: SequenceAnnotation
persistent identity: annotation2410064
display id: annotation2410064
version: 1
top level: 1GateNpuE
location: https://synbiohub.org/public/igem/BBa_K1362101/annotation2410064/range2410064/1
component: BBa_K1362418

https://synbiohub.org/public/igem/BBa_K1362101/annotation2410064/range2410064/1
type: Range
persistent identity: range2410064
display id: range2410064
version: 1
direction: direction/reverse
top level: 1GateNpuE
start: 60
end: 63
orientation: inline

BBa_G0000
type: SequenceAnnotation
persistent identity: annotation2410059
display id: annotation2410059
version: 1
top level: 1GateNpuE
location: https://synbiohub.org/public/igem/BBa_K1362101/annotation2410059/range2410059/1
component: BBa_G0000

https://synbiohub.org/public/igem/BBa_K1362101/annotation2410059/range2410059/1
type: Range
persistent identity: range2410059
display id: range2410059
version: 1
direction: direction/reverse
top level: 1GateNpuE
start: 30
end: 35
orientation: inline

BBa_K1362422
type: SequenceAnnotation
persistent identity: annotation2410089
display id: annotation2410089
version: 1
top level: 1GateNpuE
location: https://synbiohub.org/public/igem/BBa_K1362101/annotation2410089/range2410089/1
component: BBa_K1362422

https://synbiohub.org/public/igem/BBa_K1362101/annotation2410089/range2410089/1
type: Range
persistent identity: range2410089
display id: range2410089
version: 1
direction: direction/reverse
top level: 1GateNpuE
start: 171
end: 1253
orientation: inline

BBa_K1362090
type: SequenceAnnotation
persistent identity: annotation2410058
display id: annotation2410058
version: 1
top level: 1GateNpuE
location: https://synbiohub.org/public/igem/BBa_K1362101/annotation2410058/range2410058/1
component: BBa_K1362090

https://synbiohub.org/public/igem/BBa_K1362101/annotation2410058/range2410058/1
type: Range
persistent identity: range2410058
display id: range2410058
version: 1
direction: direction/reverse
top level: 1GateNpuE
start: 1
end: 29
orientation: inline

BBa_K1331004
type: ComponentDefinition
persistent identity: BBa K1331004
display id: BBa_K1331004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Modified rhlB coding sequence from Pseudomonas aeruginosa SQ6
creator: Tianyi Huang
created: 2014-09-24T11:00:00Z
modified: 2015-05-08T01:09:56Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1706_
m user id: 0
owner id: 20153
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: RhlB together with RhlA encodes Rhamnosyltransferase I for mono-rahmnolipids biosynthesis in Pseudomonas aeruginosa. This part includes bp upstream noncoding gene and bp downstreanm gene of RhlB. Two mutations have been done to remove the PstI restriction sites contained in the coding sequence, which won't change the amino acid sequence.
mutable notes: Two mutations have been done to remove the PstI restriction sites contained in the coding sequence, which won't change the amino acid sequence.
mutable provenance: Pseudomonas aeruginosa SQ6
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: noncoding sequence
sequence annotation: RhlB
sequence: https://synbiohub.org/public/igem/BBa_K1331004_sequence/1

noncoding sequence
type: SequenceAnnotation
persistent identity: annotation2434208
display id: annotation2434208
version: 1
top level: BBa_K1331004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1331004/annotation2434208/range2434208/1

https://synbiohub.org/public/igem/BBa_K1331004/annotation2434208/range2434208/1
type: Range
persistent identity: range2434208
display id: range2434208
version: 1
direction: direction/forward
top level: BBa_K1331004
start: 1
end: 6
orientation: inline

RhlB
type: SequenceAnnotation
persistent identity: annotation2434209
display id: annotation2434209
version: 1
top level: BBa_K1331004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1331004/annotation2434209/range2434209/1

https://synbiohub.org/public/igem/BBa_K1331004/annotation2434209/range2434209/1
type: Range
persistent identity: range2434209
display id: range2434209
version: 1
direction: direction/forward
top level: BBa_K1331004
start: 7
end: 1287
orientation: inline

noncoding sequence
type: SequenceAnnotation
persistent identity: annotation2434210
display id: annotation2434210
version: 1
top level: BBa_K1331004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1331004/annotation2434210/range2434210/1

https://synbiohub.org/public/igem/BBa_K1331004/annotation2434210/range2434210/1
type: Range
persistent identity: range2434210
display id: range2434210
version: 1
direction: direction/forward
top level: BBa_K1331004
start: 1288
end: 1307
orientation: inline

BBa_K1639004
type: ComponentDefinition
persistent identity: BBa K1639004
display id: BBa_K1639004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: H-NS
creator: Mustafa Yılmaz
created: 2015-09-11T11:00:00Z
modified: 2015-09-18T07:25:52Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2056_
m user id: 20835
owner id: 20835
owning group id: 9
status: status/Planning
bookmark: false
mutable description: global DNA-binding transcriptional dual regulator H-NS
mutable notes: This part contains an additional BamHI restriction site at beginning of TlpB sequence and a XhoI restriction site at the end.
mutable provenance: Escherichia Coli K-12, sequence taken from Genbank: http://www.ncbi.nlm.nih.gov/gene/945829
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: ATG Start Codon
sequence annotation: Stop Codon
sequence annotation: H-NS
sequence: https://synbiohub.org/public/igem/BBa_K1639004_sequence/1

ATG Start Codon
type: SequenceAnnotation
persistent identity: annotation2452956
display id: annotation2452956
version: 1
top level: BBa_K1639004
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1639004/annotation2452956/range2452956/1

https://synbiohub.org/public/igem/BBa_K1639004/annotation2452956/range2452956/1
type: Range
persistent identity: range2452956
display id: range2452956
version: 1
direction: direction/forward
top level: BBa_K1639004
start: 9
end: 11
orientation: inline

Stop Codon
type: SequenceAnnotation
persistent identity: annotation2452958
display id: annotation2452958
version: 1
top level: BBa_K1639004
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1639004/annotation2452958/range2452958/1

https://synbiohub.org/public/igem/BBa_K1639004/annotation2452958/range2452958/1
type: Range
persistent identity: range2452958
display id: range2452958
version: 1
direction: direction/forward
top level: BBa_K1639004
start: 420
end: 426
orientation: inline

H-NS
type: SequenceAnnotation
persistent identity: annotation2452955
display id: annotation2452955
version: 1
top level: BBa_K1639004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1639004/annotation2452955/range2452955/1

https://synbiohub.org/public/igem/BBa_K1639004/annotation2452955/range2452955/1
type: Range
persistent identity: range2452955
display id: range2452955
version: 1
direction: direction/forward
top level: BBa_K1639004
start: 9
end: 422
orientation: inline

BBa_K1763421
type: ComponentDefinition
persistent identity: BBa K1763421
display id: BBa_K1763421
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PCquad Mutant 10
creator: Tyler Lee
created: 2015-09-10T11:00:00Z
modified: 2016-10-02T06:33:24Z
discontinued: false
dominant: false
group u list: _2185_
m user id: 27134
owner id: 27528
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Subunit of a 12-subunit protein cage with a thrombin cleavage sequence inserted between residues 243 and 248 (ALPSAE to ALPGLVPRGSGSAE).
mutable notes: Considered the area to insert the cleavage sequence. This mutation was selected by examining the secondary structure to ensure minimal disruption of existing alpha-helices or beta sheets, sites were sandwiched by glycine residues, and used PyMOL to check if the site would be accessible to the protease. Geometric accessibility by the protease thrombin and minimal speculated extent of conformational changes by insertion.
mutable provenance: Bromoperoxidase (trimer) and M1 Virus Matrix Protein (dimer) linked with an alpha helix.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: Start
sequence annotation: RBS
sequence annotation: Scar
sequence annotation: Double Stop
sequence annotation: T7 Promoter
sequence annotation: PCquad Mutant 10
sequence annotation: 6x His Tag
sequence: https://synbiohub.org/public/igem/BBa_K1763421_sequence/1

Start
type: SequenceAnnotation
persistent identity: annotation2467612
display id: annotation2467612
version: 1
top level: BBa_K1763421
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1763421/annotation2467612/range2467612/1

https://synbiohub.org/public/igem/BBa_K1763421/annotation2467612/range2467612/1
type: Range
persistent identity: range2467612
display id: range2467612
version: 1
direction: direction/forward
top level: BBa_K1763421
start: 40
end: 42
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2467609
display id: annotation2467609
version: 1
top level: BBa_K1763421
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1763421/annotation2467609/range2467609/1

https://synbiohub.org/public/igem/BBa_K1763421/annotation2467609/range2467609/1
type: Range
persistent identity: range2467609
display id: range2467609
version: 1
direction: direction/forward
top level: BBa_K1763421
start: 24
end: 28
orientation: inline

Scar
type: SequenceAnnotation
persistent identity: annotation2467610
display id: annotation2467610
version: 1
top level: BBa_K1763421
role: feature/scar
location: https://synbiohub.org/public/igem/BBa_K1763421/annotation2467610/range2467610/1

https://synbiohub.org/public/igem/BBa_K1763421/annotation2467610/range2467610/1
type: Range
persistent identity: range2467610
display id: range2467610
version: 1
direction: direction/forward
top level: BBa_K1763421
start: 34
end: 39
orientation: inline

Double Stop
type: SequenceAnnotation
persistent identity: annotation2467611
display id: annotation2467611
version: 1
top level: BBa_K1763421
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1763421/annotation2467611/range2467611/1

https://synbiohub.org/public/igem/BBa_K1763421/annotation2467611/range2467611/1
type: Range
persistent identity: range2467611
display id: range2467611
version: 1
direction: direction/forward
top level: BBa_K1763421
start: 1425
end: 1430
orientation: inline

T7 Promoter
type: SequenceAnnotation
persistent identity: annotation2467608
display id: annotation2467608
version: 1
top level: BBa_K1763421
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1763421/annotation2467608/range2467608/1

https://synbiohub.org/public/igem/BBa_K1763421/annotation2467608/range2467608/1
type: Range
persistent identity: range2467608
display id: range2467608
version: 1
direction: direction/forward
top level: BBa_K1763421
start: 1
end: 22
orientation: inline

PCquad Mutant 10
type: SequenceAnnotation
persistent identity: annotation2467630
display id: annotation2467630
version: 1
top level: BBa_K1763421
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1763421/annotation2467630/range2467630/1

https://synbiohub.org/public/igem/BBa_K1763421/annotation2467630/range2467630/1
type: Range
persistent identity: range2467630
display id: range2467630
version: 1
direction: direction/forward
top level: BBa_K1763421
start: 40
end: 1385
orientation: inline

6x His Tag
type: SequenceAnnotation
persistent identity: annotation2467628
display id: annotation2467628
version: 1
top level: BBa_K1763421
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1763421/annotation2467628/range2467628/1

https://synbiohub.org/public/igem/BBa_K1763421/annotation2467628/range2467628/1
type: Range
persistent identity: range2467628
display id: range2467628
version: 1
direction: direction/forward
top level: BBa_K1763421
start: 1386
end: 1400
orientation: inline

BBa_K1315003
type: ComponentDefinition
persistent identity: BBa K1315003
display id: BBa_K1315003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: The rpfG gene from Xanthomonas campestris
creator: Dimitrios Michailidis
created: 2014-09-24T11:00:00Z
modified: 2015-05-08T01:09:48Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1690_
m user id: 0
owner id: 21812
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is the coding sequence for the RpfG(regulation of pathogenicity factor G) protein. It is the regulator in the two-component signal transduction system of Xanthomonas campestris pv. campestris that is activated by the sensor kinase RpfC(Bba_K1315002) in the presence of DSF(diffusible signal factor).
mutable notes: The sequence had 1 EcoRI site that was mutated out by site-directed mutagenesis.
mutable provenance: The source of this part is Xanthomonas campestris pv. campestris(Xcc) genomic DNA which was provided by the Robert Ryan lab in the Molecular Microbiology department of the University of Dundee, UK.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: RpfG
sequence: https://synbiohub.org/public/igem/BBa_K1315003_sequence/1

RpfG
type: SequenceAnnotation
persistent identity: annotation2386405
display id: annotation2386405
version: 1
top level: BBa_K1315003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1315003/annotation2386405/range2386405/1

https://synbiohub.org/public/igem/BBa_K1315003/annotation2386405/range2386405/1
type: Range
persistent identity: range2386405
display id: range2386405
version: 1
direction: direction/forward
top level: BBa_K1315003
start: 1
end: 1140
orientation: inline

BBa_K1632010
type: ComponentDefinition
persistent identity: BBa K1632010
display id: BBa_K1632010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: fimB (wild-type)
creator: Riku Shinohara
created: 2015-08-17T11:00:00Z
modified: 2015-09-17T02:45:06Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2049_
m user id: 24760
owner id: 22687
owning group id: 9
status: status/Planning
bookmark: false
mutable description: a
mutable notes: a
mutable provenance: a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1632010_sequence/1

3alpha-HSD
type: ComponentDefinition
persistent identity: BBa K1674001
display id: BBa_K1674001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 3alpha-hydroxysteroid dehydrogenase
creator: Liron Abrahami
created: 2015-09-12T11:00:00Z
modified: 2015-09-18T02:27:41Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2092_
m user id: 25765
owner id: 25765
owning group id: 9
status: status/Planning
bookmark: true
mutable description: 3α- HSD
mutable notes: k
mutable provenance: gblock ordered from IDT
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1674001_sequence/1

BBa_K1362005
type: ComponentDefinition
persistent identity: BBa K1362005
display id: BBa_K1362005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Sortase A non-circularization construct (with Smt3 and His6)
creator: Silvan Schmitz
created: 2014-10-08T11:00:00Z
modified: 2015-05-08T01:10:04Z
discontinued: false
dominant: false
group u list: _1738_
m user id: 0
owner id: 22951
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: TODO
mutable notes: TODO
mutable provenance: TODO
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1362205
component: BBa_K1362090
sequence annotation: BBa_K1362090
sequence annotation: BBa_K1362205
sequence: https://synbiohub.org/public/igem/BBa_K1362005_sequence/1

BBa_K1362205
type: Component
persistent identity: component2409518
display id: component2409518
version: 1
top level: BBa_K1362005
access: public
definition: BBa_K1362205

BBa_K1362090
type: Component
persistent identity: component2409486
display id: component2409486
version: 1
top level: BBa_K1362005
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362090/1

BBa_K1362090
type: SequenceAnnotation
persistent identity: annotation2409486
display id: annotation2409486
version: 1
top level: BBa_K1362005
location: https://synbiohub.org/public/igem/BBa_K1362005/annotation2409486/range2409486/1
component: BBa_K1362090

https://synbiohub.org/public/igem/BBa_K1362005/annotation2409486/range2409486/1
type: Range
persistent identity: range2409486
display id: range2409486
version: 1
direction: direction/reverse
top level: BBa_K1362005
start: 1
end: 29
orientation: inline

BBa_K1362205
type: SequenceAnnotation
persistent identity: annotation2409518
display id: annotation2409518
version: 1
top level: BBa_K1362005
location: https://synbiohub.org/public/igem/BBa_K1362005/annotation2409518/range2409518/1
component: BBa_K1362205

https://synbiohub.org/public/igem/BBa_K1362005/annotation2409518/range2409518/1
type: Range
persistent identity: range2409518
display id: range2409518
version: 1
direction: direction/reverse
top level: BBa_K1362005
start: 36
end: 1522
orientation: inline

BBa_K1763438
type: ComponentDefinition
persistent identity: BBa K1763438
display id: BBa_K1763438
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: MaSp1/2[2:1]-12(1C3)
creator: Vinson Lam
created: 2015-09-14T11:00:00Z
modified: 2015-09-16T11:57:37Z
discontinued: false
dominant: false
group u list: _2185_
m user id: 27136
owner id: 27136
owning group id: 9
status: status/Planning
bookmark: false
mutable description: MaSp1 and MaSp2 12-mer block co-polymer in pSB1C3, no regulatory elements. Ratio of 2 MaSp1 to 1 MaSp2.
mutable notes: no
mutable provenance: N. clavipes
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: M1-AB
sequence annotation: M2-BC
sequence annotation: M2-CA
sequence annotation: 8-his tag
sequence annotation: M2-AB
sequence annotation: M1-CA
sequence annotation: M1-BC
sequence: https://synbiohub.org/public/igem/BBa_K1763438_sequence/1

M1-AB
type: SequenceAnnotation
persistent identity: annotation2464088
display id: annotation2464088
version: 1
top level: BBa_K1763438
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763438/annotation2464088/range2464088/1

https://synbiohub.org/public/igem/BBa_K1763438/annotation2464088/range2464088/1
type: Range
persistent identity: range2464088
display id: range2464088
version: 1
direction: direction/forward
top level: BBa_K1763438
start: 36
end: 137
orientation: inline

M1-AB
type: SequenceAnnotation
persistent identity: annotation2464094
display id: annotation2464094
version: 1
top level: BBa_K1763438
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763438/annotation2464094/range2464094/1

https://synbiohub.org/public/igem/BBa_K1763438/annotation2464094/range2464094/1
type: Range
persistent identity: range2464094
display id: range2464094
version: 1
direction: direction/forward
top level: BBa_K1763438
start: 648
end: 749
orientation: inline

M2-BC
type: SequenceAnnotation
persistent identity: annotation2464086
display id: annotation2464086
version: 1
top level: BBa_K1763438
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763438/annotation2464086/range2464086/1

https://synbiohub.org/public/igem/BBa_K1763438/annotation2464086/range2464086/1
type: Range
persistent identity: range2464086
display id: range2464086
version: 1
direction: direction/forward
top level: BBa_K1763438
start: 1056
end: 1157
orientation: inline

M2-CA
type: SequenceAnnotation
persistent identity: annotation2464085
display id: annotation2464085
version: 1
top level: BBa_K1763438
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763438/annotation2464085/range2464085/1

https://synbiohub.org/public/igem/BBa_K1763438/annotation2464085/range2464085/1
type: Range
persistent identity: range2464085
display id: range2464085
version: 1
direction: direction/forward
top level: BBa_K1763438
start: 1158
end: 1259
orientation: inline

8-his tag
type: SequenceAnnotation
persistent identity: annotation2464087
display id: annotation2464087
version: 1
top level: BBa_K1763438
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1763438/annotation2464087/range2464087/1

https://synbiohub.org/public/igem/BBa_K1763438/annotation2464087/range2464087/1
type: Range
persistent identity: range2464087
display id: range2464087
version: 1
direction: direction/forward
top level: BBa_K1763438
start: 4
end: 27
orientation: inline

M2-AB
type: SequenceAnnotation
persistent identity: annotation2464097
display id: annotation2464097
version: 1
top level: BBa_K1763438
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763438/annotation2464097/range2464097/1

https://synbiohub.org/public/igem/BBa_K1763438/annotation2464097/range2464097/1
type: Range
persistent identity: range2464097
display id: range2464097
version: 1
direction: direction/forward
top level: BBa_K1763438
start: 954
end: 1055
orientation: inline

M1-CA
type: SequenceAnnotation
persistent identity: annotation2464093
display id: annotation2464093
version: 1
top level: BBa_K1763438
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763438/annotation2464093/range2464093/1

https://synbiohub.org/public/igem/BBa_K1763438/annotation2464093/range2464093/1
type: Range
persistent identity: range2464093
display id: range2464093
version: 1
direction: direction/forward
top level: BBa_K1763438
start: 546
end: 647
orientation: inline

M2-CA
type: SequenceAnnotation
persistent identity: annotation2464096
display id: annotation2464096
version: 1
top level: BBa_K1763438
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763438/annotation2464096/range2464096/1

https://synbiohub.org/public/igem/BBa_K1763438/annotation2464096/range2464096/1
type: Range
persistent identity: range2464096
display id: range2464096
version: 1
direction: direction/forward
top level: BBa_K1763438
start: 852
end: 953
orientation: inline

M1-AB
type: SequenceAnnotation
persistent identity: annotation2464091
display id: annotation2464091
version: 1
top level: BBa_K1763438
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763438/annotation2464091/range2464091/1

https://synbiohub.org/public/igem/BBa_K1763438/annotation2464091/range2464091/1
type: Range
persistent identity: range2464091
display id: range2464091
version: 1
direction: direction/forward
top level: BBa_K1763438
start: 342
end: 443
orientation: inline

M1-BC
type: SequenceAnnotation
persistent identity: annotation2464092
display id: annotation2464092
version: 1
top level: BBa_K1763438
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763438/annotation2464092/range2464092/1

https://synbiohub.org/public/igem/BBa_K1763438/annotation2464092/range2464092/1
type: Range
persistent identity: range2464092
display id: range2464092
version: 1
direction: direction/forward
top level: BBa_K1763438
start: 444
end: 545
orientation: inline

M1-BC
type: SequenceAnnotation
persistent identity: annotation2464089
display id: annotation2464089
version: 1
top level: BBa_K1763438
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763438/annotation2464089/range2464089/1

https://synbiohub.org/public/igem/BBa_K1763438/annotation2464089/range2464089/1
type: Range
persistent identity: range2464089
display id: range2464089
version: 1
direction: direction/forward
top level: BBa_K1763438
start: 138
end: 239
orientation: inline

M1-CA
type: SequenceAnnotation
persistent identity: annotation2464090
display id: annotation2464090
version: 1
top level: BBa_K1763438
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763438/annotation2464090/range2464090/1

https://synbiohub.org/public/igem/BBa_K1763438/annotation2464090/range2464090/1
type: Range
persistent identity: range2464090
display id: range2464090
version: 1
direction: direction/forward
top level: BBa_K1763438
start: 240
end: 341
orientation: inline

M1-BC
type: SequenceAnnotation
persistent identity: annotation2464095
display id: annotation2464095
version: 1
top level: BBa_K1763438
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763438/annotation2464095/range2464095/1

https://synbiohub.org/public/igem/BBa_K1763438/annotation2464095/range2464095/1
type: Range
persistent identity: range2464095
display id: range2464095
version: 1
direction: direction/forward
top level: BBa_K1763438
start: 750
end: 851
orientation: inline

BBa_K1674002
type: ComponentDefinition
persistent identity: BBa K1674002
display id: BBa_K1674002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pT7 - RBS - 3αHSD
creator: Adi Yannai
created: 2015-09-14T11:00:00Z
modified: 2015-09-17T03:07:01Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2092_
m user id: 25765
owner id: 25765
owning group id: 9
status: status/Planning
bookmark: false
mutable description: pT7 - 3αHSD
mutable notes: pT7 - 3αHSD
mutable provenance: pT7 - 3αHSD
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1674001
component: BBa_K1321338
sequence annotation: BBa_K1321338
sequence annotation: BBa_K1674001
sequence: https://synbiohub.org/public/igem/BBa_K1674002_sequence/1

BBa_K1674001
type: Component
persistent identity: component2467114
display id: component2467114
version: 1
top level: BBa_K1674002
access: public
definition: 3alpha-HSD

BBa_K1321338
type: Component
persistent identity: component2467113
display id: component2467113
version: 1
top level: BBa_K1674002
access: public
definition: BBa_K1321338

BBa_K1321338
type: SequenceAnnotation
persistent identity: annotation2467113
display id: annotation2467113
version: 1
top level: BBa_K1674002
location: https://synbiohub.org/public/igem/BBa_K1674002/annotation2467113/range2467113/1
component: BBa_K1321338

https://synbiohub.org/public/igem/BBa_K1674002/annotation2467113/range2467113/1
type: Range
persistent identity: range2467113
display id: range2467113
version: 1
direction: direction/reverse
top level: BBa_K1674002
start: 1
end: 43
orientation: inline

BBa_K1674001
type: SequenceAnnotation
persistent identity: annotation2467114
display id: annotation2467114
version: 1
top level: BBa_K1674002
location: https://synbiohub.org/public/igem/BBa_K1674002/annotation2467114/range2467114/1
component: BBa_K1674001

https://synbiohub.org/public/igem/BBa_K1674002/annotation2467114/range2467114/1
type: Range
persistent identity: range2467114
display id: range2467114
version: 1
direction: direction/reverse
top level: BBa_K1674002
start: 50
end: 1018
orientation: inline

Art175
type: ComponentDefinition
persistent identity: BBa K1659000
display id: BBa_K1659000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Artilysin Art-175, an antibacterial fusion protein
creator: Wei Chung Kong
created: 2015-08-27T11:00:00Z
modified: 2016-02-01T12:51:21Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2077_
m user id: 4206
owner id: 24524
owning group id: 9
status: status/Planning
bookmark: false
mutable description: to be updated
mutable notes: to be updated
mutable provenance: to be updated
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Art-175
sequence annotation: Mistake
sequence annotation: Start
sequence annotation: Hisx6
sequence annotation: Stop
sequence: https://synbiohub.org/public/igem/BBa_K1659000_sequence/1

Art-175
type: SequenceAnnotation
persistent identity: annotation2440111
display id: annotation2440111
version: 1
top level: Art175
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1659000/annotation2440111/range2440111/1

https://synbiohub.org/public/igem/BBa_K1659000/annotation2440111/range2440111/1
type: Range
persistent identity: range2440111
display id: range2440111
version: 1
direction: direction/forward
top level: Art175
start: 7
end: 873
orientation: inline

Mistake
type: SequenceAnnotation
persistent identity: annotation2456677
display id: annotation2456677
version: 1
top level: Art175
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K1659000/annotation2456677/range2456677/1

https://synbiohub.org/public/igem/BBa_K1659000/annotation2456677/range2456677/1
type: Range
persistent identity: range2456677
display id: range2456677
version: 1
direction: direction/forward
top level: Art175
start: 4
end: 6
orientation: inline

Start
type: SequenceAnnotation
persistent identity: annotation2440110
display id: annotation2440110
version: 1
top level: Art175
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1659000/annotation2440110/range2440110/1

https://synbiohub.org/public/igem/BBa_K1659000/annotation2440110/range2440110/1
type: Range
persistent identity: range2440110
display id: range2440110
version: 1
direction: direction/forward
top level: Art175
start: 1
end: 3
orientation: inline

Hisx6
type: SequenceAnnotation
persistent identity: annotation2440112
display id: annotation2440112
version: 1
top level: Art175
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1659000/annotation2440112/range2440112/1

https://synbiohub.org/public/igem/BBa_K1659000/annotation2440112/range2440112/1
type: Range
persistent identity: range2440112
display id: range2440112
version: 1
direction: direction/forward
top level: Art175
start: 874
end: 891
orientation: inline

Stop
type: SequenceAnnotation
persistent identity: annotation2440113
display id: annotation2440113
version: 1
top level: Art175
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1659000/annotation2440113/range2440113/1

https://synbiohub.org/public/igem/BBa_K1659000/annotation2440113/range2440113/1
type: Range
persistent identity: range2440113
display id: range2440113
version: 1
direction: direction/forward
top level: Art175
start: 892
end: 897
orientation: inline

BBa_K1758335
type: ComponentDefinition
persistent identity: BBa K1758335
display id: BBa_K1758335
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: UTR-sfGFP controlled by T7-promoter and lead responsive promoter
creator: Team Bielefeld-CeBiTec 2015
created: 2015-09-08T11:00:00Z
modified: 2016-01-06T03:41:29Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2180_
m user id: 4206
owner id: 26630
owning group id: 9
status: status/Planning
bookmark: false
mutable description: lead induceble promoter under the control of T7 Promoter with mRFP for detection
mutable notes: ...
mutable provenance: This part consists of T7 promoter lead induceble Promoter PbrA from ''Cupriavidus metallidurans CH34'' and mRFP for detection
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_I719005
component: BBa_K1758331
sequence annotation: BBa_K1758331
sequence annotation: BBa_I719005
sequence: https://synbiohub.org/public/igem/BBa_K1758335_sequence/1

BBa_I719005
type: Component
persistent identity: component2447787
display id: component2447787
version: 1
top level: BBa_K1758335
access: public
definition: https://synbiohub.org/public/igem/BBa_I719005/1

BBa_K1758331
type: Component
persistent identity: component2447790
display id: component2447790
version: 1
top level: BBa_K1758335
access: public
definition: BBa_K1758331

BBa_K1758331
type: SequenceAnnotation
persistent identity: annotation2447790
display id: annotation2447790
version: 1
top level: BBa_K1758335
location: https://synbiohub.org/public/igem/BBa_K1758335/annotation2447790/range2447790/1
component: BBa_K1758331

https://synbiohub.org/public/igem/BBa_K1758335/annotation2447790/range2447790/1
type: Range
persistent identity: range2447790
display id: range2447790
version: 1
direction: direction/reverse
top level: BBa_K1758335
start: 32
end: 786
orientation: inline

BBa_I719005
type: SequenceAnnotation
persistent identity: annotation2447787
display id: annotation2447787
version: 1
top level: BBa_K1758335
location: https://synbiohub.org/public/igem/BBa_K1758335/annotation2447787/range2447787/1
component: BBa_I719005

https://synbiohub.org/public/igem/BBa_K1758335/annotation2447787/range2447787/1
type: Range
persistent identity: range2447787
display id: range2447787
version: 1
direction: direction/reverse
top level: BBa_K1758335
start: 1
end: 23
orientation: inline

BBa_K1758342
type: ComponentDefinition
persistent identity: BBa K1758342
display id: BBa_K1758342
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Mercury responsive promoter with UTR-sfGFP
creator: Team Bielefeld-CeBiTec 2015
created: 2015-08-28T11:00:00Z
modified: 2016-01-06T03:41:22Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2180_
m user id: 4206
owner id: 26630
owning group id: 9
status: status/Planning
bookmark: false
mutable description: mercury responsive promoter with 5??untranslated region and sfGFP for detection
mutable notes: ...
mutable provenance: modified after K346002
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: T1
sequence annotation: T7-term
sequence annotation: 5??UTR
sequence annotation: merT
sequence annotation: sfGFP
sequence: https://synbiohub.org/public/igem/BBa_K1758342_sequence/1

T1
type: SequenceAnnotation
persistent identity: annotation2439969
display id: annotation2439969
version: 1
top level: BBa_K1758342
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1758342/annotation2439969/range2439969/1

https://synbiohub.org/public/igem/BBa_K1758342/annotation2439969/range2439969/1
type: Range
persistent identity: range2439969
display id: range2439969
version: 1
direction: direction/forward
top level: BBa_K1758342
start: 838
end: 917
orientation: inline

T7-term
type: SequenceAnnotation
persistent identity: annotation2439970
display id: annotation2439970
version: 1
top level: BBa_K1758342
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1758342/annotation2439970/range2439970/1

https://synbiohub.org/public/igem/BBa_K1758342/annotation2439970/range2439970/1
type: Range
persistent identity: range2439970
display id: range2439970
version: 1
direction: direction/forward
top level: BBa_K1758342
start: 926
end: 966
orientation: inline

5??UTR
type: SequenceAnnotation
persistent identity: annotation2439967
display id: annotation2439967
version: 1
top level: BBa_K1758342
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1758342/annotation2439967/range2439967/1

https://synbiohub.org/public/igem/BBa_K1758342/annotation2439967/range2439967/1
type: Range
persistent identity: range2439967
display id: range2439967
version: 1
direction: direction/forward
top level: BBa_K1758342
start: 64
end: 109
orientation: inline

merT
type: SequenceAnnotation
persistent identity: annotation2439966
display id: annotation2439966
version: 1
top level: BBa_K1758342
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1758342/annotation2439966/range2439966/1

https://synbiohub.org/public/igem/BBa_K1758342/annotation2439966/range2439966/1
type: Range
persistent identity: range2439966
display id: range2439966
version: 1
direction: direction/forward
top level: BBa_K1758342
start: 1
end: 57
orientation: inline

sfGFP
type: SequenceAnnotation
persistent identity: annotation2439968
display id: annotation2439968
version: 1
top level: BBa_K1758342
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1758342/annotation2439968/range2439968/1

https://synbiohub.org/public/igem/BBa_K1758342/annotation2439968/range2439968/1
type: Range
persistent identity: range2439968
display id: range2439968
version: 1
direction: direction/forward
top level: BBa_K1758342
start: 110
end: 829
orientation: inline

cadA
type: ComponentDefinition
persistent identity: BBa K1602003
display id: BBa_K1602003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cis-aconitate decarboxylase (cadA) from Aspergillus terreus
creator: Alexandra Goretzki, Tanja Habeck, Nina Kuschik-Maczollek, Laurin Monnheimer, Steven Pilger, Bianca Reisinger, Daniel Stumpf, Sebastian Barthel
created: 2015-08-27T11:00:00Z
modified: 2015-09-25T01:18:30Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2019_
m user id: 12512
owner id: 25989
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Blank
mutable notes: Blank
mutable provenance: Aspergillus terreus
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1602003_sequence/1

BBa_K1321009
type: ComponentDefinition
persistent identity: BBa K1321009
display id: BBa_K1321009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Nickel Binding Protein (NiBP) Freiburg format (RFC[25])
creator: Chris N Micklem
created: 2014-10-07T11:00:00Z
modified: 2015-05-08T01:09:51Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 0
owner id: 20830
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Nickel Binding Protein in Freiburg format (RFC 25) to allow for easy use in fusion proteins.
mutable notes: OEPCR primers were designed to allow for the replacement of the standard BioBrick prefix and suffix with the Freiburg prefix and suffix.
mutable provenance: This part is based on the existing BioBrick BBa_K1151001 by the 2013 Salente Lecce iGEM team.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Nickel Binding Protein
sequence: https://synbiohub.org/public/igem/BBa_K1321009_sequence/1

Nickel Binding Protein
type: SequenceAnnotation
persistent identity: annotation2416941
display id: annotation2416941
version: 1
top level: BBa_K1321009
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1321009/annotation2416941/range2416941/1

https://synbiohub.org/public/igem/BBa_K1321009/annotation2416941/range2416941/1
type: Range
persistent identity: range2416941
display id: range2416941
version: 1
direction: direction/forward
top level: BBa_K1321009
start: 1
end: 176
orientation: inline

xynA-PDZli
type: ComponentDefinition
persistent identity: BBa K1602035
display id: BBa_K1602035
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Inducible generator of RBS-xynA-PDZlig
creator: Jasmin Weber, Michelle Zoeller, Marcus Geissler, Stefan Bohn, Simon Linder, Sebastian Barthel
created: 2015-09-15T11:00:00Z
modified: 2015-09-16T07:45:09Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2019_
m user id: 22022
owner id: 22022
owning group id: 9
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K1602033
component: BBa_I719005
sequence annotation: BBa_I719005
sequence annotation: BBa_K1602033
sequence: https://synbiohub.org/public/igem/BBa_K1602035_sequence/1

BBa_K1602033
type: Component
persistent identity: component2463047
display id: component2463047
version: 1
top level: xynA-PDZli
access: public
definition: https://synbiohub.org/public/igem/BBa_K1602033/1

BBa_I719005
type: Component
persistent identity: component2463046
display id: component2463046
version: 1
top level: xynA-PDZli
access: public
definition: https://synbiohub.org/public/igem/BBa_I719005/1

BBa_I719005
type: SequenceAnnotation
persistent identity: annotation2463046
display id: annotation2463046
version: 1
top level: xynA-PDZli
location: https://synbiohub.org/public/igem/BBa_K1602035/annotation2463046/range2463046/1
component: BBa_I719005

https://synbiohub.org/public/igem/BBa_K1602035/annotation2463046/range2463046/1
type: Range
persistent identity: range2463046
display id: range2463046
version: 1
direction: direction/reverse
top level: xynA-PDZli
start: 1
end: 23
orientation: inline

BBa_K1602033
type: SequenceAnnotation
persistent identity: annotation2463047
display id: annotation2463047
version: 1
top level: xynA-PDZli
location: https://synbiohub.org/public/igem/BBa_K1602035/annotation2463047/range2463047/1
component: BBa_K1602033

https://synbiohub.org/public/igem/BBa_K1602035/annotation2463047/range2463047/1
type: Range
persistent identity: range2463047
display id: range2463047
version: 1
direction: direction/reverse
top level: xynA-PDZli
start: 32
end: 724
orientation: inline

BBa_K1355004
type: ComponentDefinition
persistent identity: BBa K1355004
display id: BBa_K1355004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Mercury ions bioremediating device
creator: Luna Barroco de Lacerda, Maria Clara Tavares Astolfi
created: 2014-10-05T11:00:00Z
modified: 2015-05-08T01:10:02Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1731_
m user id: 0
owner id: 15499
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: The biorremediator device is composed by our ???essential biobrick??? (BBa_K1355001) + a MerA gene (BBa_K1355000). In the presence of mercury, The MerR protein will bind to it and MerT, MerP and MerA protein will be expressed and bioremediation will start! The MerP and MerT proteins are responsible for the transport of mercury from the periplasm to the cytoplasm, leading him to the mercury ion reductase (MerA)! The beautiful MerA be responsible for the reduction from Hg2+ in Hg0, which is volatile at room temperature and able to passively leave out the bacteria.
mutable notes: For this construct, we followed these steps: Extraction of plasmid DNA and DNA quantification of bacteria transformed with the Essential Biobrick (BBa_K1355001) and the bacteria transformed with the MerA Biobrick (BBa_E0840); Verifying the electrophoretic profile of the extracted plasmid DNA; Restriction enzyme digestion for Essential Biobrick with SpeI and PstI and for merA Biobrick with PstI and XbaI; Checking the electrophoretic profile of digested samples; Fragment of interest purification from the gel; Ligation the gel purification of merA gene and digested Essential Biobrick; Transformation of ligation in DH5-alpha; Plasmid DNA extraction from bacteria transformed; Check the electrophoretic profile to see results of samples linked (no fragments); Digestion of Essential Biobrick + merA Biobrick with EcoRI and PstI, aiming to analyze the fragment size to be isolated; Checking the electrophoretic profile of the digested sample to obtain results showing that the isolated fragment is the junction of Essential Biobrick +merA Biobrick ;
mutable provenance: BBa_K1355001, BBa_K1355000
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K1355000
component: BBa_K1355001
sequence annotation: BBa_K1355000
sequence annotation: BBa_K1355001
sequence: https://synbiohub.org/public/igem/BBa_K1355004_sequence/1

BBa_K1355000
type: Component
persistent identity: component2415200
display id: component2415200
version: 1
top level: BBa_K1355004
access: public
definition: https://synbiohub.org/public/igem/BBa_K1355000/1

BBa_K1355001
type: Component
persistent identity: component2415196
display id: component2415196
version: 1
top level: BBa_K1355004
access: public
definition: https://synbiohub.org/public/igem/BBa_K1355001/1

BBa_K1355000
type: SequenceAnnotation
persistent identity: annotation2415200
display id: annotation2415200
version: 1
top level: BBa_K1355004
location: https://synbiohub.org/public/igem/BBa_K1355004/annotation2415200/range2415200/1
component: BBa_K1355000

https://synbiohub.org/public/igem/BBa_K1355004/annotation2415200/range2415200/1
type: Range
persistent identity: range2415200
display id: range2415200
version: 1
direction: direction/reverse
top level: BBa_K1355004
start: 1198
end: 2975
orientation: inline

BBa_K1355001
type: SequenceAnnotation
persistent identity: annotation2415196
display id: annotation2415196
version: 1
top level: BBa_K1355004
location: https://synbiohub.org/public/igem/BBa_K1355004/annotation2415196/range2415196/1
component: BBa_K1355001

https://synbiohub.org/public/igem/BBa_K1355004/annotation2415196/range2415196/1
type: Range
persistent identity: range2415196
display id: range2415196
version: 1
direction: direction/reverse
top level: BBa_K1355004
start: 1
end: 1189
orientation: inline

BBa_K1499201
type: ComponentDefinition
persistent identity: BBa K1499201
display id: BBa_K1499201
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Deinococcus radiodurans uracil-DNA glycosylase 1
creator: Raman Nelakanti
created: 2014-08-24T11:00:00Z
modified: 2015-05-08T01:10:46Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1879_
m user id: 0
owner id: 11095
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: TODO
mutable notes: TODO
mutable provenance: Deinococcus radiodurans
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: TAA Stop
sequence annotation: uracil-DNA glycosylase 1
sequence annotation: Start codon
sequence: https://synbiohub.org/public/igem/BBa_K1499201_sequence/1

TAA Stop
type: SequenceAnnotation
persistent identity: annotation2381351
display id: annotation2381351
version: 1
top level: BBa_K1499201
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1499201/annotation2381351/range2381351/1

https://synbiohub.org/public/igem/BBa_K1499201/annotation2381351/range2381351/1
type: Range
persistent identity: range2381351
display id: range2381351
version: 1
direction: direction/forward
top level: BBa_K1499201
start: 742
end: 744
orientation: inline

uracil-DNA glycosylase 1
type: SequenceAnnotation
persistent identity: annotation2429020
display id: annotation2429020
version: 1
top level: BBa_K1499201
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1499201/annotation2429020/range2429020/1

https://synbiohub.org/public/igem/BBa_K1499201/annotation2429020/range2429020/1
type: Range
persistent identity: range2429020
display id: range2429020
version: 1
direction: direction/forward
top level: BBa_K1499201
start: 1
end: 744
orientation: inline

Start codon
type: SequenceAnnotation
persistent identity: annotation2381350
display id: annotation2381350
version: 1
top level: BBa_K1499201
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1499201/annotation2381350/range2381350/1

https://synbiohub.org/public/igem/BBa_K1499201/annotation2381350/range2381350/1
type: Range
persistent identity: range2381350
display id: range2381350
version: 1
direction: direction/forward
top level: BBa_K1499201
start: 1
end: 3
orientation: inline

BBa_K1696005
type: ComponentDefinition
persistent identity: BBa K1696005
display id: BBa_K1696005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: P170 Promoter
creator: Yue Liu
created: 2015-09-10T11:00:00Z
modified: 2015-09-11T12:43:19Z
discontinued: false
dominant: false
group u list: _2114_
m user id: 16881
owner id: 16881
owning group id: 9
status: status/Planning
bookmark: false
mutable description: P170 promoter is upregulated at low pH during the transition to stationary phase.P170 was induced between pH 6.5 and pH 6.0.
mutable notes: P170 segment contains a cis-acting sequence involved in the control of promoter regulation.
mutable provenance: L. lactis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: P170 promoter
sequence: https://synbiohub.org/public/igem/BBa_K1696005_sequence/1

P170 promoter
type: SequenceAnnotation
persistent identity: annotation2449400
display id: annotation2449400
version: 1
top level: BBa_K1696005
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1696005/annotation2449400/range2449400/1

https://synbiohub.org/public/igem/BBa_K1696005/annotation2449400/range2449400/1
type: Range
persistent identity: range2449400
display id: range2449400
version: 1
direction: direction/forward
top level: BBa_K1696005
start: 1
end: 418
orientation: inline

BBa_K1497029
type: ComponentDefinition
persistent identity: BBa K1497029
display id: BBa_K1497029
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Scaffold (S-P)
creator: Tobias Gabriel, Rene Sahm
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:46Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1877_
m user id: 0
owner id: 12466
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Scaffold (S-P)
mutable notes: Scaffold (S-P)
mutable provenance: Scaffold (S-P)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1497029_sequence/1

BBa_K1460003
type: ComponentDefinition
persistent identity: BBa K1460003
display id: BBa_K1460003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Anderson Promoter + NixA + Ter
creator: Eric Holmes
created: 2014-08-13T11:00:00Z
modified: 2016-01-13T10:21:44Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _1839_
m user id: 4206
owner id: 15649
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is a functional coding sequence for the protein NixA, which is a nickel transporter from H. pylori. This part will result in nickel intolerance from higher levels of intracellular nickel.
mutable notes: The Anderson promoter provides strong constitutive expression of NixA in E. coli.
mutable provenance: Anderson promoter is that of part BBa_J23102. Terminator is that of part BBa_B1006. NixA gene is naturally found in H. pylori. This part was synthesized by GenScript in the vector pUC57. Part was then transferred into pSB1C3 using standard cloning procedures.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: BBa_B1006
sequence annotation: BBa_J61101
sequence annotation: NixA
sequence annotation: BBa_J23102
sequence: https://synbiohub.org/public/igem/BBa_K1460003_sequence/1

BBa_B1006
type: SequenceAnnotation
persistent identity: annotation2381016
display id: annotation2381016
version: 1
top level: BBa_K1460003
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1460003/annotation2381016/range2381016/1

https://synbiohub.org/public/igem/BBa_K1460003/annotation2381016/range2381016/1
type: Range
persistent identity: range2381016
display id: range2381016
version: 1
direction: direction/forward
top level: BBa_K1460003
start: 990
end: 1028
orientation: inline

BBa_J61101
type: SequenceAnnotation
persistent identity: annotation2381014
display id: annotation2381014
version: 1
top level: BBa_K1460003
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1460003/annotation2381014/range2381014/1

https://synbiohub.org/public/igem/BBa_K1460003/annotation2381014/range2381014/1
type: Range
persistent identity: range2381014
display id: range2381014
version: 1
direction: direction/forward
top level: BBa_K1460003
start: 43
end: 56
orientation: inline

NixA
type: SequenceAnnotation
persistent identity: annotation2381015
display id: annotation2381015
version: 1
top level: BBa_K1460003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1460003/annotation2381015/range2381015/1

https://synbiohub.org/public/igem/BBa_K1460003/annotation2381015/range2381015/1
type: Range
persistent identity: range2381015
display id: range2381015
version: 1
direction: direction/forward
top level: BBa_K1460003
start: 63
end: 989
orientation: inline

BBa_J23102
type: SequenceAnnotation
persistent identity: annotation2381013
display id: annotation2381013
version: 1
top level: BBa_K1460003
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1460003/annotation2381013/range2381013/1

https://synbiohub.org/public/igem/BBa_K1460003/annotation2381013/range2381013/1
type: Range
persistent identity: range2381013
display id: range2381013
version: 1
direction: direction/forward
top level: BBa_K1460003
start: 1
end: 35
orientation: inline

BBa_K1602011
type: ComponentDefinition
persistent identity: BBa K1602011
display id: BBa_K1602011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: yagE
creator: Leon Kraus, Jonathan Pletzer-Zelgert, Jonas Sindlinger, Carmen Sakas Gandullo, Sebastian Holzner, Elena Tonchevska, Mirjam Haun
created: 2015-09-13T11:00:00Z
modified: 2015-09-18T06:39:03Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2019_
m user id: 26220
owner id: 25878
owning group id: 9
status: status/Planning
bookmark: false
mutable description: BLANK
mutable notes: BLANK
mutable provenance: BLANK
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1602011_sequence/1

BBa_K1602046
type: ComponentDefinition
persistent identity: BBa K1602046
display id: BBa_K1602046
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RRK3
creator: Christian Sator,Stefan Zens, Max Zander, Benedikt Spannenkrebs, Sebastian Jaeger
created: 2015-09-15T11:00:00Z
modified: 2015-09-25T05:16:35Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _2019_
m user id: 0
m user id: 23028
owner id: 26220
owning group id: 9
sample status: In stock
status: status/Available
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J01086
component: BBa_K808000
component: BBa_B0015
sequence annotation: BBa_J01086
sequence annotation: BBa_B0015
sequence annotation: BBa_K808000
sequence: https://synbiohub.org/public/igem/BBa_K1602046_sequence/1

BBa_J01086
type: Component
persistent identity: component2462831
display id: component2462831
version: 1
top level: BBa_K1602046
access: public
definition: https://synbiohub.org/public/igem/BBa_J01086/1

BBa_K808000
type: Component
persistent identity: component2462830
display id: component2462830
version: 1
top level: BBa_K1602046
access: public
definition: https://synbiohub.org/public/igem/BBa_K808000/1

BBa_B0015
type: Component
persistent identity: component2462838
display id: component2462838
version: 1
top level: BBa_K1602046
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_J01086
type: SequenceAnnotation
persistent identity: annotation2462831
display id: annotation2462831
version: 1
top level: BBa_K1602046
location: https://synbiohub.org/public/igem/BBa_K1602046/annotation2462831/range2462831/1
component: BBa_J01086

https://synbiohub.org/public/igem/BBa_K1602046/annotation2462831/range2462831/1
type: Range
persistent identity: range2462831
display id: range2462831
version: 1
direction: direction/reverse
top level: BBa_K1602046
start: 1218
end: 1311
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2462838
display id: annotation2462838
version: 1
top level: BBa_K1602046
location: https://synbiohub.org/public/igem/BBa_K1602046/annotation2462838/range2462838/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1602046/annotation2462838/range2462838/1
type: Range
persistent identity: range2462838
display id: range2462838
version: 1
direction: direction/reverse
top level: BBa_K1602046
start: 1320
end: 1448
orientation: inline

BBa_K808000
type: SequenceAnnotation
persistent identity: annotation2462830
display id: annotation2462830
version: 1
top level: BBa_K1602046
location: https://synbiohub.org/public/igem/BBa_K1602046/annotation2462830/range2462830/1
component: BBa_K808000

https://synbiohub.org/public/igem/BBa_K1602046/annotation2462830/range2462830/1
type: Range
persistent identity: range2462830
display id: range2462830
version: 1
direction: direction/reverse
top level: BBa_K1602046
start: 1
end: 1209
orientation: inline

BBa_K1334000
type: ComponentDefinition
persistent identity: BBa K1334000
display id: BBa_K1334000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: A promoter activitated by formaldehyde.
creator: Haoyuan Sun
created: 2014-09-25T11:00:00Z
modified: 2015-05-08T01:09:58Z
discontinued: false
dominant: false
group u list: _1709_
m user id: 0
owner id: 22430
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This promoter can sense the whether there is formaldehyde or not.It can be activitated by formaldehyde with the help of the protein hxlR.This promoter may have some leakage in the strain E.coli DH5a .
mutable notes: There may be different expression levels when you use it in different stains of E.coli.
mutable provenance: This part comes from the Bacillus subtilis genomic sequence.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: A theoretically promoter(very weak)
sequence annotation: HxlR protein binding site 2
sequence annotation: HxlR protein binding site 1
sequence annotation: rbs
sequence annotation: Formaldehyde promoter
sequence: https://synbiohub.org/public/igem/BBa_K1334000_sequence/1

A theoretically promoter(very weak)
type: SequenceAnnotation
persistent identity: annotation2387005
display id: annotation2387005
version: 1
top level: BBa_K1334000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1334000/annotation2387005/range2387005/1

https://synbiohub.org/public/igem/BBa_K1334000/annotation2387005/range2387005/1
type: Range
persistent identity: range2387005
display id: range2387005
version: 1
direction: direction/reverse
top level: BBa_K1334000
start: 73
end: 107
orientation: inline

HxlR protein binding site 2
type: SequenceAnnotation
persistent identity: annotation2387004
display id: annotation2387004
version: 1
top level: BBa_K1334000
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1334000/annotation2387004/range2387004/1

https://synbiohub.org/public/igem/BBa_K1334000/annotation2387004/range2387004/1
type: Range
persistent identity: range2387004
display id: range2387004
version: 1
direction: direction/forward
top level: BBa_K1334000
start: 151
end: 175
orientation: inline

HxlR protein binding site 1
type: SequenceAnnotation
persistent identity: annotation2387003
display id: annotation2387003
version: 1
top level: BBa_K1334000
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1334000/annotation2387003/range2387003/1

https://synbiohub.org/public/igem/BBa_K1334000/annotation2387003/range2387003/1
type: Range
persistent identity: range2387003
display id: range2387003
version: 1
direction: direction/forward
top level: BBa_K1334000
start: 126
end: 150
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2386999
display id: annotation2386999
version: 1
top level: BBa_K1334000
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1334000/annotation2386999/range2386999/1

https://synbiohub.org/public/igem/BBa_K1334000/annotation2386999/range2386999/1
type: Range
persistent identity: range2386999
display id: range2386999
version: 1
direction: direction/forward
top level: BBa_K1334000
start: 215
end: 222
orientation: inline

Formaldehyde promoter
type: SequenceAnnotation
persistent identity: annotation2387002
display id: annotation2387002
version: 1
top level: BBa_K1334000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1334000/annotation2387002/range2387002/1

https://synbiohub.org/public/igem/BBa_K1334000/annotation2387002/range2387002/1
type: Range
persistent identity: range2387002
display id: range2387002
version: 1
direction: direction/forward
top level: BBa_K1334000
start: 171
end: 207
orientation: inline

DsbDspBx
type: ComponentDefinition
persistent identity: BBa K1659211
display id: BBa_K1659211
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Dispersin B fused with DsbA, with mutation at nucleotide 636 (T to C)
creator: Wei Chung Kong
created: 2015-08-27T11:00:00Z
modified: 2015-09-14T04:16:28Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2077_
m user id: 24524
owner id: 24524
owning group id: 9
status: status/Planning
bookmark: false
mutable description: to be updated
mutable notes: to be updated
mutable provenance: to be updated
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: Hisx6
sequence annotation: DspBx
sequence annotation: stop
sequence annotation: DsbA 2-19 signal sequence
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_K1659211_sequence/1

Hisx6
type: SequenceAnnotation
persistent identity: annotation2452808
display id: annotation2452808
version: 1
top level: DsbDspBx
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1659211/annotation2452808/range2452808/1

https://synbiohub.org/public/igem/BBa_K1659211/annotation2452808/range2452808/1
type: Range
persistent identity: range2452808
display id: range2452808
version: 1
direction: direction/forward
top level: DsbDspBx
start: 1141
end: 1158
orientation: inline

DspBx
type: SequenceAnnotation
persistent identity: annotation2452807
display id: annotation2452807
version: 1
top level: DsbDspBx
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1659211/annotation2452807/range2452807/1

https://synbiohub.org/public/igem/BBa_K1659211/annotation2452807/range2452807/1
type: Range
persistent identity: range2452807
display id: range2452807
version: 1
direction: direction/forward
top level: DsbDspBx
start: 58
end: 1140
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2452809
display id: annotation2452809
version: 1
top level: DsbDspBx
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1659211/annotation2452809/range2452809/1

https://synbiohub.org/public/igem/BBa_K1659211/annotation2452809/range2452809/1
type: Range
persistent identity: range2452809
display id: range2452809
version: 1
direction: direction/forward
top level: DsbDspBx
start: 1159
end: 1164
orientation: inline

DsbA 2-19 signal sequence
type: SequenceAnnotation
persistent identity: annotation2452806
display id: annotation2452806
version: 1
top level: DsbDspBx
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1659211/annotation2452806/range2452806/1

https://synbiohub.org/public/igem/BBa_K1659211/annotation2452806/range2452806/1
type: Range
persistent identity: range2452806
display id: range2452806
version: 1
direction: direction/forward
top level: DsbDspBx
start: 4
end: 57
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2452805
display id: annotation2452805
version: 1
top level: DsbDspBx
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1659211/annotation2452805/range2452805/1

https://synbiohub.org/public/igem/BBa_K1659211/annotation2452805/range2452805/1
type: Range
persistent identity: range2452805
display id: range2452805
version: 1
direction: direction/forward
top level: DsbDspBx
start: 1
end: 3
orientation: inline

BBa_K1321162
type: ComponentDefinition
persistent identity: BBa K1321162
display id: BBa_K1321162
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NiBP driven by LacI promoter
creator: Gabriella Santosa
created: 2014-10-08T11:00:00Z
modified: 2015-05-08T01:09:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 0
owner id: 20961
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: GATC
mutable notes: GATC
mutable provenance: GATC
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1319106
component: BBa_K1321009
component: BBa_J04500
sequence annotation: BBa_K1319106
sequence annotation: BBa_K1321009
sequence annotation: BBa_J04500
sequence: https://synbiohub.org/public/igem/BBa_K1321162_sequence/1

BBa_K1319106
type: Component
persistent identity: component2415068
display id: component2415068
version: 1
top level: BBa_K1321162
access: public
definition: https://synbiohub.org/public/igem/BBa_K1319106/1

BBa_K1321009
type: Component
persistent identity: component2415069
display id: component2415069
version: 1
top level: BBa_K1321162
access: public
definition: BBa_K1321009

BBa_J04500
type: Component
persistent identity: component2415066
display id: component2415066
version: 1
top level: BBa_K1321162
access: public
definition: https://synbiohub.org/public/igem/BBa_J04500/1

BBa_K1319106
type: SequenceAnnotation
persistent identity: annotation2415068
display id: annotation2415068
version: 1
top level: BBa_K1321162
location: https://synbiohub.org/public/igem/BBa_K1321162/annotation2415068/range2415068/1
component: BBa_K1319106

https://synbiohub.org/public/igem/BBa_K1321162/annotation2415068/range2415068/1
type: Range
persistent identity: range2415068
display id: range2415068
version: 1
direction: direction/reverse
top level: BBa_K1321162
start: 221
end: 235
orientation: inline

BBa_K1321009
type: SequenceAnnotation
persistent identity: annotation2415069
display id: annotation2415069
version: 1
top level: BBa_K1321162
location: https://synbiohub.org/public/igem/BBa_K1321162/annotation2415069/range2415069/1
component: BBa_K1321009

https://synbiohub.org/public/igem/BBa_K1321162/annotation2415069/range2415069/1
type: Range
persistent identity: range2415069
display id: range2415069
version: 1
direction: direction/reverse
top level: BBa_K1321162
start: 236
end: 411
orientation: inline

BBa_J04500
type: SequenceAnnotation
persistent identity: annotation2415066
display id: annotation2415066
version: 1
top level: BBa_K1321162
location: https://synbiohub.org/public/igem/BBa_K1321162/annotation2415066/range2415066/1
component: BBa_J04500

https://synbiohub.org/public/igem/BBa_K1321162/annotation2415066/range2415066/1
type: Range
persistent identity: range2415066
display id: range2415066
version: 1
direction: direction/reverse
top level: BBa_K1321162
start: 1
end: 220
orientation: inline

BBa_K1659501
type: ComponentDefinition
persistent identity: BBa K1659501
display id: BBa_K1659501
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pLsr + RBS + sfGFP
creator: Wei Chung Kong
created: 2015-08-27T11:00:00Z
modified: 2015-09-14T09:35:38Z
discontinued: false
dominant: false
group u list: _2077_
m user id: 24524
owner id: 24524
owning group id: 9
status: status/Planning
bookmark: false
mutable description: to be updated
mutable notes: to be updated
mutable provenance: to be updated
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: Weak RBS (B0031)
sequence annotation: Superfolder GFP (I746916)
sequence annotation: stop
sequence annotation: pLsr (K117002)
sequence: https://synbiohub.org/public/igem/BBa_K1659501_sequence/1

Weak RBS (B0031)
type: SequenceAnnotation
persistent identity: annotation2447908
display id: annotation2447908
version: 1
top level: BBa_K1659501
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1659501/annotation2447908/range2447908/1

https://synbiohub.org/public/igem/BBa_K1659501/annotation2447908/range2447908/1
type: Range
persistent identity: range2447908
display id: range2447908
version: 1
direction: direction/forward
top level: BBa_K1659501
start: 99
end: 112
orientation: inline

Superfolder GFP (I746916)
type: SequenceAnnotation
persistent identity: annotation2447909
display id: annotation2447909
version: 1
top level: BBa_K1659501
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1659501/annotation2447909/range2447909/1

https://synbiohub.org/public/igem/BBa_K1659501/annotation2447909/range2447909/1
type: Range
persistent identity: range2447909
display id: range2447909
version: 1
direction: direction/forward
top level: BBa_K1659501
start: 119
end: 838
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2447910
display id: annotation2447910
version: 1
top level: BBa_K1659501
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1659501/annotation2447910/range2447910/1

https://synbiohub.org/public/igem/BBa_K1659501/annotation2447910/range2447910/1
type: Range
persistent identity: range2447910
display id: range2447910
version: 1
direction: direction/forward
top level: BBa_K1659501
start: 839
end: 844
orientation: inline

pLsr (K117002)
type: SequenceAnnotation
persistent identity: annotation2447907
display id: annotation2447907
version: 1
top level: BBa_K1659501
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1659501/annotation2447907/range2447907/1

https://synbiohub.org/public/igem/BBa_K1659501/annotation2447907/range2447907/1
type: Range
persistent identity: range2447907
display id: range2447907
version: 1
direction: direction/forward
top level: BBa_K1659501
start: 1
end: 90
orientation: inline

BBa_K1758300
type: ComponentDefinition
persistent identity: BBa K1758300
display id: BBa_K1758300
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: sfGFP controlled by T7 promoter and arsenic operator
creator: Team Bielefeld-CeBiTec 2015
created: 2015-08-21T11:00:00Z
modified: 2015-09-18T04:46:17Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2180_
m user id: 27935
owner id: 26587
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This part consists of sfGFP with a translation enhancing 5'-UTR under the control of the T7 promoter and arsenic operator. In combination with the E. coli arsenic repressor arsR, this part can be used as an arsenic biosensor.
mutable notes: This part was created by inserting the T7 promoter BBa_I719005 upstream of BBa_J33201 via 3A assembly. Subsequently, Gibson assembly was performed to replace arsR by sfGFP and introduce the UTR. The followoing primers and templates were used: Fragment 1: UTR_arsO_rev: ccttcttttttttttttaaagttaaacaaaattatTAAGTCAAAAACATATATGACTTAACG Cm_fwd: CGGCATCAGCACCTTGTC Template: pSB1C3-pT7-arsR Fragment 2: UTR_fwd: GTTTAACTTTAAAAAAAAAAAAGAAGGAGATATACATATGCGTAAAGGCGAAGAGCTGTTCAC Cm_rev: TATACGCAAGGCGACAAG Template: BBa_I746909
mutable provenance: Based on three parts from the distribution.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: sfGFP
sequence annotation: T7 promoter
sequence annotation: 5'-UTR
sequence annotation: ArsR binding site
sequence annotation: BBa_I719005
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K1758300_sequence/1

sfGFP
type: SequenceAnnotation
persistent identity: annotation2436867
display id: annotation2436867
version: 1
top level: BBa_K1758300
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1758300/annotation2436867/range2436867/1

https://synbiohub.org/public/igem/BBa_K1758300/annotation2436867/range2436867/1
type: Range
persistent identity: range2436867
display id: range2436867
version: 1
direction: direction/forward
top level: BBa_K1758300
start: 150
end: 866
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2436865
display id: annotation2436865
version: 1
top level: BBa_K1758300
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1758300/annotation2436865/range2436865/1

https://synbiohub.org/public/igem/BBa_K1758300/annotation2436865/range2436865/1
type: Range
persistent identity: range2436865
display id: range2436865
version: 1
direction: direction/forward
top level: BBa_K1758300
start: 1
end: 23
orientation: inline

5'-UTR
type: SequenceAnnotation
persistent identity: annotation2436872
display id: annotation2436872
version: 1
top level: BBa_K1758300
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1758300/annotation2436872/range2436872/1

https://synbiohub.org/public/igem/BBa_K1758300/annotation2436872/range2436872/1
type: Range
persistent identity: range2436872
display id: range2436872
version: 1
direction: direction/forward
top level: BBa_K1758300
start: 104
end: 149
orientation: inline

ArsR binding site
type: SequenceAnnotation
persistent identity: annotation2436871
display id: annotation2436871
version: 1
top level: BBa_K1758300
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K1758300/annotation2436871/range2436871/1

https://synbiohub.org/public/igem/BBa_K1758300/annotation2436871/range2436871/1
type: Range
persistent identity: range2436871
display id: range2436871
version: 1
direction: direction/forward
top level: BBa_K1758300
start: 67
end: 85
orientation: inline

BBa_I719005
type: SequenceAnnotation
persistent identity: annotation2436866
display id: annotation2436866
version: 1
top level: BBa_K1758300
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1758300/annotation2436866/range2436866/1

https://synbiohub.org/public/igem/BBa_K1758300/annotation2436866/range2436866/1
type: Range
persistent identity: range2436866
display id: range2436866
version: 1
direction: direction/forward
top level: BBa_K1758300
start: 1
end: 23
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2436870
display id: annotation2436870
version: 1
top level: BBa_K1758300
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1758300/annotation2436870/range2436870/1

https://synbiohub.org/public/igem/BBa_K1758300/annotation2436870/range2436870/1
type: Range
persistent identity: range2436870
display id: range2436870
version: 1
direction: direction/forward
top level: BBa_K1758300
start: 966
end: 1006
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2436869
display id: annotation2436869
version: 1
top level: BBa_K1758300
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1758300/annotation2436869/range2436869/1

https://synbiohub.org/public/igem/BBa_K1758300/annotation2436869/range2436869/1
type: Range
persistent identity: range2436869
display id: range2436869
version: 1
direction: direction/forward
top level: BBa_K1758300
start: 878
end: 957
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2436868
display id: annotation2436868
version: 1
top level: BBa_K1758300
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1758300/annotation2436868/range2436868/1

https://synbiohub.org/public/igem/BBa_K1758300/annotation2436868/range2436868/1
type: Range
persistent identity: range2436868
display id: range2436868
version: 1
direction: direction/forward
top level: BBa_K1758300
start: 137
end: 144
orientation: inline

BBa_K1321005
type: ComponentDefinition
persistent identity: BBa K1321005
display id: BBa_K1321005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Synthetic Phytochelatin EC(20) RFC[25]
creator: Xenia Spencer-Milnes
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:09:51Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 0
owner id: 20780
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: Synthetic Phytochelatin EC(20) rfc25 fusion format
mutable notes: Codon optimised for E.coli.
mutable provenance: Sequence is not natural. Basis for the sequence was from the following reference: Bae W, Chen W, Mulchandani A, Mehra RK (2000) Enhanced bioaccumulation of heavy metals by bacterial cells displaying synthetic phytochelatins. Biotechnol Bioeng. 70(5):518-24.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Synthetic Phytochelatin EC(20)
sequence: https://synbiohub.org/public/igem/BBa_K1321005_sequence/1

Synthetic Phytochelatin EC(20)
type: SequenceAnnotation
persistent identity: annotation2414598
display id: annotation2414598
version: 1
top level: BBa_K1321005
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1321005/annotation2414598/range2414598/1

https://synbiohub.org/public/igem/BBa_K1321005/annotation2414598/range2414598/1
type: Range
persistent identity: range2414598
display id: range2414598
version: 1
direction: direction/forward
top level: BBa_K1321005
start: 1
end: 123
orientation: inline

BBa_K1321339
type: ComponentDefinition
persistent identity: BBa K1321339
display id: BBa_K1321339
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CBDcenA+Linker, RFC 25 standard
creator: Chris N Micklem
created: 2014-10-07T11:00:00Z
modified: 2015-06-17T12:14:18Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 4206
owner id: 20830
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: CBDcenA in Freiburg format (RFC 25).
mutable notes: asdf
mutable provenance: asdf
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Linker: Pro-Thr box
sequence annotation: CBDCenA
sequence: https://synbiohub.org/public/igem/BBa_K1321339_sequence/1

Linker: Pro-Thr box
type: SequenceAnnotation
persistent identity: annotation2414614
display id: annotation2414614
version: 1
top level: BBa_K1321339
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1321339/annotation2414614/range2414614/1

https://synbiohub.org/public/igem/BBa_K1321339/annotation2414614/range2414614/1
type: Range
persistent identity: range2414614
display id: range2414614
version: 1
direction: direction/forward
top level: BBa_K1321339
start: 319
end: 408
orientation: inline

CBDCenA
type: SequenceAnnotation
persistent identity: annotation2418065
display id: annotation2418065
version: 1
top level: BBa_K1321339
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1321339/annotation2418065/range2418065/1

https://synbiohub.org/public/igem/BBa_K1321339/annotation2418065/range2418065/1
type: Range
persistent identity: range2418065
display id: range2418065
version: 1
direction: direction/forward
top level: BBa_K1321339
start: 1
end: 318
orientation: inline

BBa_K1763439
type: ComponentDefinition
persistent identity: BBa K1763439
display id: BBa_K1763439
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: MaSp1/2[2:1]-12(T7)
creator: Vinson Lam
created: 2015-09-14T11:00:00Z
modified: 2015-09-15T09:39:57Z
discontinued: false
dominant: false
group u list: _2185_
m user id: 27136
owner id: 27136
owning group id: 9
status: status/Planning
bookmark: false
mutable description: MaSp1 and MaSp2 12-mer block co-polymer under control of T7 promoter and RBS. Ratio of 2 MaSp1 to 1 MaSp2.
mutable notes: no
mutable provenance: N. clavipes
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K1763438
component: BBa_K525998
sequence annotation: BBa_K525998
sequence annotation: BBa_K1763438
sequence: https://synbiohub.org/public/igem/BBa_K1763439_sequence/1

BBa_K1763438
type: Component
persistent identity: component2456898
display id: component2456898
version: 1
top level: BBa_K1763439
access: public
definition: BBa_K1763438

BBa_K525998
type: Component
persistent identity: component2456897
display id: component2456897
version: 1
top level: BBa_K1763439
access: public
definition: https://synbiohub.org/public/igem/BBa_K525998/1

BBa_K525998
type: SequenceAnnotation
persistent identity: annotation2456897
display id: annotation2456897
version: 1
top level: BBa_K1763439
location: https://synbiohub.org/public/igem/BBa_K1763439/annotation2456897/range2456897/1
component: BBa_K525998

https://synbiohub.org/public/igem/BBa_K1763439/annotation2456897/range2456897/1
type: Range
persistent identity: range2456897
display id: range2456897
version: 1
direction: direction/reverse
top level: BBa_K1763439
start: 1
end: 32
orientation: inline

BBa_K1763438
type: SequenceAnnotation
persistent identity: annotation2456898
display id: annotation2456898
version: 1
top level: BBa_K1763439
location: https://synbiohub.org/public/igem/BBa_K1763439/annotation2456898/range2456898/1
component: BBa_K1763438

https://synbiohub.org/public/igem/BBa_K1763439/annotation2456898/range2456898/1
type: Range
persistent identity: range2456898
display id: range2456898
version: 1
direction: direction/reverse
top level: BBa_K1763439
start: 39
end: 1313
orientation: inline

BBa_K1331003
type: ComponentDefinition
persistent identity: BBa K1331003
display id: BBa_K1331003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RhlI Acyl-homoserine-lactone synthase from P.aeruginosa SQ6
creator: Jiayi Dong
created: 2014-09-25T11:00:00Z
modified: 2015-05-08T01:09:56Z
discontinued: false
dominant: false
group u list: _1706_
m user id: 0
owner id: 20167
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This sequece encodes RhlI, an acyl-homoserine-lactone synthase required for the synthesis of BHL (N-butanoyl-L-homoserine lactone), and HHL (N-hexanoyl-L-homoserine lactone) autoinducer molecules which bind to RhlR.
mutable notes: It includes both the complete coding sequence of RhlI and part of noncoding sequence between RhlR and RhlI gene.
mutable provenance: It comes from the genomic sequence of P.aeruginosa SQ6.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: RhlI
sequence annotation: noncoding sequence
sequence: https://synbiohub.org/public/igem/BBa_K1331003_sequence/1

RhlI
type: SequenceAnnotation
persistent identity: annotation2427973
display id: annotation2427973
version: 1
top level: BBa_K1331003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1331003/annotation2427973/range2427973/1

https://synbiohub.org/public/igem/BBa_K1331003/annotation2427973/range2427973/1
type: Range
persistent identity: range2427973
display id: range2427973
version: 1
direction: direction/forward
top level: BBa_K1331003
start: 114
end: 719
orientation: inline

noncoding sequence
type: SequenceAnnotation
persistent identity: annotation2427981
display id: annotation2427981
version: 1
top level: BBa_K1331003
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1331003/annotation2427981/range2427981/1

https://synbiohub.org/public/igem/BBa_K1331003/annotation2427981/range2427981/1
type: Range
persistent identity: range2427981
display id: range2427981
version: 1
direction: direction/forward
top level: BBa_K1331003
start: 1
end: 113
orientation: inline

BBa_K1343022
type: ComponentDefinition
persistent identity: BBa K1343022
display id: BBa_K1343022
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Plux->amilCP->tetR
creator: Tal Naor, Ricarina Rabinovitz, Ittai Rubinstein, Yair Maimon
created: 2014-10-05T11:00:00Z
modified: 2015-05-08T01:10:00Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1718_
m user id: 0
owner id: 20669
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: The promoter Plux in induced by the dimer AHL-luxR. As a result, amilCP, a blue chromophoire protein, is synthesized. TetR is a repressor of the promoter Ptet.
mutable notes: r
mutable provenance: BBa_R0062: Plux, BBa_B0030: Strong RBS, BBa_K592009: amilCP, BBa_B0010:T1 (terminator) from E. coli.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K1343022_sequence/1

BBa_K1583100
type: ComponentDefinition
persistent identity: BBa K1583100
display id: BBa_K1583100
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pRha + CsgA
creator: Max van 't Hof
created: 2015-09-02T11:00:00Z
modified: 2015-09-18T11:09:29Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2000_
m user id: 24478
owner id: 24478
owning group id: 9
status: status/Planning
bookmark: false
mutable description: CsgA under control of L-rhamnose-inducible promoter
mutable notes: The nucleotides encoding for the second and third amino acid of the CsgA gene were changed to optimize synthesis success. (silent mutations)
mutable provenance: This part was synthesized. The rhamnose promoter was used by the iGEM 2014 TU Delft teama and originally added by the iGEM12 Paris Bettencourt team. RBS and CsgA originate from E. coli K-12 MG1655
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1583000
component: BBa_K1583008
component: BBa_K1583051
component: BBa_G0002
component: BBa_K914003
component: BBa_K1583050
sequence annotation: BBa_K1583000
sequence annotation: BBa_K1583051
sequence annotation: BBa_G0002
sequence annotation: BBa_K1583050
sequence annotation: BBa_K914003
sequence annotation: BBa_K1583008
sequence: https://synbiohub.org/public/igem/BBa_K1583100_sequence/1

BBa_K1583000
type: Component
persistent identity: component2443489
display id: component2443489
version: 1
top level: BBa_K1583100
access: public
definition: https://synbiohub.org/public/igem/BBa_K1583000/1

BBa_K1583008
type: Component
persistent identity: component2443487
display id: component2443487
version: 1
top level: BBa_K1583100
access: public
definition: https://synbiohub.org/public/igem/BBa_K1583008/1

BBa_K1583051
type: Component
persistent identity: component2443490
display id: component2443490
version: 1
top level: BBa_K1583100
access: public
definition: https://synbiohub.org/public/igem/BBa_K1583051/1

BBa_G0002
type: Component
persistent identity: component2443483
display id: component2443483
version: 1
top level: BBa_K1583100
access: public
definition: https://synbiohub.org/public/igem/BBa_G0002/1

BBa_K914003
type: Component
persistent identity: component2443485
display id: component2443485
version: 1
top level: BBa_K1583100
access: public
definition: https://synbiohub.org/public/igem/BBa_K914003/1

BBa_K1583050
type: Component
persistent identity: component2443486
display id: component2443486
version: 1
top level: BBa_K1583100
access: public
definition: https://synbiohub.org/public/igem/BBa_K1583050/1

BBa_K1583000
type: SequenceAnnotation
persistent identity: annotation2443489
display id: annotation2443489
version: 1
top level: BBa_K1583100
location: https://synbiohub.org/public/igem/BBa_K1583100/annotation2443489/range2443489/1
component: BBa_K1583000

https://synbiohub.org/public/igem/BBa_K1583100/annotation2443489/range2443489/1
type: Range
persistent identity: range2443489
display id: range2443489
version: 1
direction: direction/reverse
top level: BBa_K1583100
start: 169
end: 624
orientation: inline

BBa_K1583051
type: SequenceAnnotation
persistent identity: annotation2443490
display id: annotation2443490
version: 1
top level: BBa_K1583100
location: https://synbiohub.org/public/igem/BBa_K1583100/annotation2443490/range2443490/1
component: BBa_K1583051

https://synbiohub.org/public/igem/BBa_K1583100/annotation2443490/range2443490/1
type: Range
persistent identity: range2443490
display id: range2443490
version: 1
direction: direction/reverse
top level: BBa_K1583100
start: 625
end: 638
orientation: inline

BBa_G0002
type: SequenceAnnotation
persistent identity: annotation2443483
display id: annotation2443483
version: 1
top level: BBa_K1583100
location: https://synbiohub.org/public/igem/BBa_K1583100/annotation2443483/range2443483/1
component: BBa_G0002

https://synbiohub.org/public/igem/BBa_K1583100/annotation2443483/range2443483/1
type: Range
persistent identity: range2443483
display id: range2443483
version: 1
direction: direction/reverse
top level: BBa_K1583100
start: 1
end: 8
orientation: inline

BBa_K1583050
type: SequenceAnnotation
persistent identity: annotation2443486
display id: annotation2443486
version: 1
top level: BBa_K1583100
location: https://synbiohub.org/public/igem/BBa_K1583100/annotation2443486/range2443486/1
component: BBa_K1583050

https://synbiohub.org/public/igem/BBa_K1583100/annotation2443486/range2443486/1
type: Range
persistent identity: range2443486
display id: range2443486
version: 1
direction: direction/reverse
top level: BBa_K1583100
start: 131
end: 155
orientation: inline

BBa_K914003
type: SequenceAnnotation
persistent identity: annotation2443485
display id: annotation2443485
version: 1
top level: BBa_K1583100
location: https://synbiohub.org/public/igem/BBa_K1583100/annotation2443485/range2443485/1
component: BBa_K914003

https://synbiohub.org/public/igem/BBa_K1583100/annotation2443485/range2443485/1
type: Range
persistent identity: range2443485
display id: range2443485
version: 1
direction: direction/reverse
top level: BBa_K1583100
start: 9
end: 130
orientation: inline

BBa_K1583008
type: SequenceAnnotation
persistent identity: annotation2443487
display id: annotation2443487
version: 1
top level: BBa_K1583100
location: https://synbiohub.org/public/igem/BBa_K1583100/annotation2443487/range2443487/1
component: BBa_K1583008

https://synbiohub.org/public/igem/BBa_K1583100/annotation2443487/range2443487/1
type: Range
persistent identity: range2443487
display id: range2443487
version: 1
direction: direction/reverse
top level: BBa_K1583100
start: 156
end: 167
orientation: inline

BBa_K1632005
type: ComponentDefinition
persistent identity: BBa K1632005
display id: BBa_K1632005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: fim switch[default OFF](wild-type)
creator: Riku Shinohara
created: 2015-08-29T11:00:00Z
modified: 2015-09-15T12:54:57Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2049_
m user id: 24760
owner id: 22687
owning group id: 9
status: status/Planning
bookmark: false
mutable description: a
mutable notes: a
mutable provenance: a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K1632005_sequence/1

BBa_K1321357
type: ComponentDefinition
persistent identity: BBa K1321357
display id: BBa_K1321357
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: sfGFP fused to CBDcex driven by LacI
creator: Chris N Micklem
created: 2014-10-07T11:00:00Z
modified: 2016-01-26T09:45:49Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 4206
owner id: 20830
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: asdf
mutable notes: asdf
mutable provenance: asdf
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1321342
component: BBa_J04500
component: BBa_K1319106
sequence annotation: BBa_K1319106
sequence annotation: BBa_J04500
sequence annotation: BBa_K1321342
sequence: https://synbiohub.org/public/igem/BBa_K1321357_sequence/1

BBa_K1321342
type: Component
persistent identity: component2415507
display id: component2415507
version: 1
top level: BBa_K1321357
access: public
definition: BBa_K1321342

BBa_J04500
type: Component
persistent identity: component2415499
display id: component2415499
version: 1
top level: BBa_K1321357
access: public
definition: https://synbiohub.org/public/igem/BBa_J04500/1

BBa_K1319106
type: Component
persistent identity: component2415501
display id: component2415501
version: 1
top level: BBa_K1321357
access: public
definition: https://synbiohub.org/public/igem/BBa_K1319106/1

BBa_K1319106
type: SequenceAnnotation
persistent identity: annotation2415501
display id: annotation2415501
version: 1
top level: BBa_K1321357
location: https://synbiohub.org/public/igem/BBa_K1321357/annotation2415501/range2415501/1
component: BBa_K1319106

https://synbiohub.org/public/igem/BBa_K1321357/annotation2415501/range2415501/1
type: Range
persistent identity: range2415501
display id: range2415501
version: 1
direction: direction/reverse
top level: BBa_K1321357
start: 221
end: 235
orientation: inline

BBa_J04500
type: SequenceAnnotation
persistent identity: annotation2415499
display id: annotation2415499
version: 1
top level: BBa_K1321357
location: https://synbiohub.org/public/igem/BBa_K1321357/annotation2415499/range2415499/1
component: BBa_J04500

https://synbiohub.org/public/igem/BBa_K1321357/annotation2415499/range2415499/1
type: Range
persistent identity: range2415499
display id: range2415499
version: 1
direction: direction/reverse
top level: BBa_K1321357
start: 1
end: 220
orientation: inline

BBa_K1321342
type: SequenceAnnotation
persistent identity: annotation2415507
display id: annotation2415507
version: 1
top level: BBa_K1321357
location: https://synbiohub.org/public/igem/BBa_K1321357/annotation2415507/range2415507/1
component: BBa_K1321342

https://synbiohub.org/public/igem/BBa_K1321357/annotation2415507/range2415507/1
type: Range
persistent identity: range2415507
display id: range2415507
version: 1
direction: direction/reverse
top level: BBa_K1321357
start: 236
end: 1279
orientation: inline

BBa_K1362172
type: ComponentDefinition
persistent identity: BBa K1362172
display id: BBa_K1362172
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Split sfGFP reconstitution C-terminal non-splicing control half
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Buescher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schaefer, Carolin Schmelas, Silvan Schmitz, Max Waldhauer
created: 2014-10-09T11:00:00Z
modified: 2015-05-08T01:10:05Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1738_
m user id: 0
owner id: 12377
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: ???
mutable notes: ???
mutable provenance: ???
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_K1362472
component: BBa_K371056
component: BBa_J18909
component: BBa_K1362479
component: BBa_K1362090
component: BBa_G0000
sequence annotation: BBa_K1362479
sequence annotation: BBa_K1362472
sequence annotation: BBa_K371056
sequence annotation: BBa_G0000
sequence annotation: BBa_J18909
sequence annotation: BBa_K1362090
sequence: https://synbiohub.org/public/igem/BBa_K1362172_sequence/1

BBa_K1362472
type: Component
persistent identity: component2414573
display id: component2414573
version: 1
top level: BBa_K1362172
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362472/1

BBa_K371056
type: Component
persistent identity: component2414566
display id: component2414566
version: 1
top level: BBa_K1362172
access: public
definition: https://synbiohub.org/public/igem/BBa_K371056/1

BBa_J18909
type: Component
persistent identity: component2414568
display id: component2414568
version: 1
top level: BBa_K1362172
access: public
definition: https://synbiohub.org/public/igem/BBa_J18909/1

BBa_K1362479
type: Component
persistent identity: component2414569
display id: component2414569
version: 1
top level: BBa_K1362172
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362479/1

BBa_K1362090
type: Component
persistent identity: component2414563
display id: component2414563
version: 1
top level: BBa_K1362172
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362090/1

BBa_G0000
type: Component
persistent identity: component2414564
display id: component2414564
version: 1
top level: BBa_K1362172
access: public
definition: https://synbiohub.org/public/igem/BBa_G0000/1

BBa_K1362479
type: SequenceAnnotation
persistent identity: annotation2414569
display id: annotation2414569
version: 1
top level: BBa_K1362172
location: https://synbiohub.org/public/igem/BBa_K1362172/annotation2414569/range2414569/1
component: BBa_K1362479

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type: Range
persistent identity: range2414569
display id: range2414569
version: 1
direction: direction/reverse
top level: BBa_K1362172
start: 57
end: 161
orientation: inline

BBa_K1362472
type: SequenceAnnotation
persistent identity: annotation2414573
display id: annotation2414573
version: 1
top level: BBa_K1362172
location: https://synbiohub.org/public/igem/BBa_K1362172/annotation2414573/range2414573/1
component: BBa_K1362472

https://synbiohub.org/public/igem/BBa_K1362172/annotation2414573/range2414573/1
type: Range
persistent identity: range2414573
display id: range2414573
version: 1
direction: direction/reverse
top level: BBa_K1362172
start: 162
end: 707
orientation: inline

BBa_K371056
type: SequenceAnnotation
persistent identity: annotation2414566
display id: annotation2414566
version: 1
top level: BBa_K1362172
location: https://synbiohub.org/public/igem/BBa_K1362172/annotation2414566/range2414566/1
component: BBa_K371056

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type: Range
persistent identity: range2414566
display id: range2414566
version: 1
direction: direction/reverse
top level: BBa_K1362172
start: 36
end: 38
orientation: inline

BBa_G0000
type: SequenceAnnotation
persistent identity: annotation2414564
display id: annotation2414564
version: 1
top level: BBa_K1362172
location: https://synbiohub.org/public/igem/BBa_K1362172/annotation2414564/range2414564/1
component: BBa_G0000

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type: Range
persistent identity: range2414564
display id: range2414564
version: 1
direction: direction/reverse
top level: BBa_K1362172
start: 30
end: 35
orientation: inline

BBa_J18909
type: SequenceAnnotation
persistent identity: annotation2414568
display id: annotation2414568
version: 1
top level: BBa_K1362172
location: https://synbiohub.org/public/igem/BBa_K1362172/annotation2414568/range2414568/1
component: BBa_J18909

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type: Range
persistent identity: range2414568
display id: range2414568
version: 1
direction: direction/reverse
top level: BBa_K1362172
start: 39
end: 56
orientation: inline

BBa_K1362090
type: SequenceAnnotation
persistent identity: annotation2414563
display id: annotation2414563
version: 1
top level: BBa_K1362172
location: https://synbiohub.org/public/igem/BBa_K1362172/annotation2414563/range2414563/1
component: BBa_K1362090

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type: Range
persistent identity: range2414563
display id: range2414563
version: 1
direction: direction/reverse
top level: BBa_K1362172
start: 1
end: 29
orientation: inline

BBa_K1602055
type: ComponentDefinition
persistent identity: BBa K1602055
display id: BBa_K1602055
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: araCpBad-GFP
creator: Christian Sator,Stefan Zens, Max Zander, Benedikt Spannenkrebs, Sebastian Jaeger
created: 2015-09-15T11:00:00Z
modified: 2015-09-25T04:55:36Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2019_
m user id: 23028
owner id: 26220
owning group id: 9
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0034
component: BBa_K808000
component: BBa_E0040
sequence annotation: BBa_E0040
sequence annotation: BBa_K808000
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K1602055_sequence/1

BBa_B0034
type: Component
persistent identity: component2462750
display id: component2462750
version: 1
top level: BBa_K1602055
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K808000
type: Component
persistent identity: component2462748
display id: component2462748
version: 1
top level: BBa_K1602055
access: public
definition: https://synbiohub.org/public/igem/BBa_K808000/1

BBa_E0040
type: Component
persistent identity: component2462752
display id: component2462752
version: 1
top level: BBa_K1602055
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2462752
display id: annotation2462752
version: 1
top level: BBa_K1602055
location: https://synbiohub.org/public/igem/BBa_K1602055/annotation2462752/range2462752/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K1602055/annotation2462752/range2462752/1
type: Range
persistent identity: range2462752
display id: range2462752
version: 1
direction: direction/reverse
top level: BBa_K1602055
start: 1236
end: 1955
orientation: inline

BBa_K808000
type: SequenceAnnotation
persistent identity: annotation2462748
display id: annotation2462748
version: 1
top level: BBa_K1602055
location: https://synbiohub.org/public/igem/BBa_K1602055/annotation2462748/range2462748/1
component: BBa_K808000

https://synbiohub.org/public/igem/BBa_K1602055/annotation2462748/range2462748/1
type: Range
persistent identity: range2462748
display id: range2462748
version: 1
direction: direction/reverse
top level: BBa_K1602055
start: 1
end: 1209
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2462750
display id: annotation2462750
version: 1
top level: BBa_K1602055
location: https://synbiohub.org/public/igem/BBa_K1602055/annotation2462750/range2462750/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1602055/annotation2462750/range2462750/1
type: Range
persistent identity: range2462750
display id: range2462750
version: 1
direction: direction/reverse
top level: BBa_K1602055
start: 1218
end: 1229
orientation: inline

BBa_K1850010
type: ComponentDefinition
persistent identity: BBa K1850010
display id: BBa_K1850010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pRha - fimH 136 KO - RPMrel - SpyTag_225 - HisTag_225
creator: Lydia Goldberg
created: 2015-09-14T11:00:00Z
modified: 2015-09-16T10:47:41Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2277_
m user id: 26421
owner id: 26421
owning group id: 9
status: status/Planning
bookmark: false
mutable description: placeholder
mutable notes: placeholder
mutable provenance: placeholder
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: B0034
sequence annotation: pRha
sequence annotation: RPMrel
sequence annotation: HisTag
sequence annotation: SpyTag
sequence annotation: fimH
sequence: https://synbiohub.org/public/igem/BBa_K1850010_sequence/1

B0034
type: SequenceAnnotation
persistent identity: annotation2463783
display id: annotation2463783
version: 1
top level: BBa_K1850010
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1850010/annotation2463783/range2463783/1

https://synbiohub.org/public/igem/BBa_K1850010/annotation2463783/range2463783/1
type: Range
persistent identity: range2463783
display id: range2463783
version: 1
direction: direction/forward
top level: BBa_K1850010
start: 252
end: 263
orientation: inline

pRha
type: SequenceAnnotation
persistent identity: annotation2463782
display id: annotation2463782
version: 1
top level: BBa_K1850010
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1850010/annotation2463782/range2463782/1

https://synbiohub.org/public/igem/BBa_K1850010/annotation2463782/range2463782/1
type: Range
persistent identity: range2463782
display id: range2463782
version: 1
direction: direction/forward
top level: BBa_K1850010
start: 4
end: 243
orientation: inline

RPMrel
type: SequenceAnnotation
persistent identity: annotation2463785
display id: annotation2463785
version: 1
top level: BBa_K1850010
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1850010/annotation2463785/range2463785/1

https://synbiohub.org/public/igem/BBa_K1850010/annotation2463785/range2463785/1
type: Range
persistent identity: range2463785
display id: range2463785
version: 1
direction: direction/forward
top level: BBa_K1850010
start: 333
end: 359
orientation: inline

HisTag
type: SequenceAnnotation
persistent identity: annotation2463787
display id: annotation2463787
version: 1
top level: BBa_K1850010
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1850010/annotation2463787/range2463787/1

https://synbiohub.org/public/igem/BBa_K1850010/annotation2463787/range2463787/1
type: Range
persistent identity: range2463787
display id: range2463787
version: 1
direction: direction/forward
top level: BBa_K1850010
start: 1071
end: 1088
orientation: inline

SpyTag
type: SequenceAnnotation
persistent identity: annotation2463786
display id: annotation2463786
version: 1
top level: BBa_K1850010
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1850010/annotation2463786/range2463786/1

https://synbiohub.org/public/igem/BBa_K1850010/annotation2463786/range2463786/1
type: Range
persistent identity: range2463786
display id: range2463786
version: 1
direction: direction/forward
top level: BBa_K1850010
start: 1032
end: 1070
orientation: inline

fimH
type: SequenceAnnotation
persistent identity: annotation2463784
display id: annotation2463784
version: 1
top level: BBa_K1850010
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1850010/annotation2463784/range2463784/1

https://synbiohub.org/public/igem/BBa_K1850010/annotation2463784/range2463784/1
type: Range
persistent identity: range2463784
display id: range2463784
version: 1
direction: direction/forward
top level: BBa_K1850010
start: 270
end: 1256
orientation: inline

BBa_K1602009
type: ComponentDefinition
persistent identity: BBa K1602009
display id: BBa_K1602009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: xylB
creator: Leon Kraus, Jonathan Pletzer-Zelgert, Jonas Sindlinger, Carmen Sakas Gandullo, Sebastian Holzner, Elena Tonchevska, Mirjam Haun
created: 2015-09-10T11:00:00Z
modified: 2015-09-25T01:22:39Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2019_
m user id: 12512
owner id: 25989
owning group id: 9
status: status/Planning
bookmark: false
mutable description: BLANK
mutable notes: BLANK
mutable provenance: BLANK
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1602009_sequence/1

BBa_K1806004
type: ComponentDefinition
persistent identity: BBa K1806004
display id: BBa_K1806004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7+ ibPB RNA Thermometer + pelB + MCS + 6xHis
creator: İBRAHİM YASİR ORHAN
created: 2015-09-16T11:00:00Z
modified: 2015-09-20T11:13:33Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2231_
m user id: 11155
owner id: 11153
owning group id: 9
status: status/Planning
bookmark: true
mutable description: The part is to act as a backbone for the T7+ibPB RNA Thermometer+pelB+6xHis system. The MCS(Multiple Cloning Site) as the ligation region for the parts to be integrated into the system. The part that is added to the backbone will be translated in the presence of higher temperatures. The pelB signal peptide is present for the produced protein to be carried out of the cell. The 6xHis is for protein analysis.
mutable notes: none
mutable provenance: This is a composite part formed up of distinct parts from distinct origins.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence: https://synbiohub.org/public/igem/BBa_K1806004_sequence/1

BBa_K1321159
type: ComponentDefinition
persistent identity: BBa K1321159
display id: BBa_K1321159
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Fmt metallothionein with T7 promoter
creator: Gabriella Santosa
created: 2014-10-08T11:00:00Z
modified: 2015-05-08T01:09:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 0
owner id: 20961
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: GATC
mutable notes: GATC
mutable provenance: GATC
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1319106
component: BBa_K1321012
component: BBa_K1321338
sequence annotation: BBa_K1321338
sequence annotation: BBa_K1319106
sequence annotation: BBa_K1321012
sequence: https://synbiohub.org/public/igem/BBa_K1321159_sequence/1

BBa_K1319106
type: Component
persistent identity: component2414967
display id: component2414967
version: 1
top level: BBa_K1321159
access: public
definition: https://synbiohub.org/public/igem/BBa_K1319106/1

BBa_K1321012
type: Component
persistent identity: component2414969
display id: component2414969
version: 1
top level: BBa_K1321159
access: public
definition: https://synbiohub.org/public/igem/BBa_K1321012/1

BBa_K1321338
type: Component
persistent identity: component2414965
display id: component2414965
version: 1
top level: BBa_K1321159
access: public
definition: BBa_K1321338

BBa_K1321338
type: SequenceAnnotation
persistent identity: annotation2414965
display id: annotation2414965
version: 1
top level: BBa_K1321159
location: https://synbiohub.org/public/igem/BBa_K1321159/annotation2414965/range2414965/1
component: BBa_K1321338

https://synbiohub.org/public/igem/BBa_K1321159/annotation2414965/range2414965/1
type: Range
persistent identity: range2414965
display id: range2414965
version: 1
direction: direction/reverse
top level: BBa_K1321159
start: 1
end: 43
orientation: inline

BBa_K1319106
type: SequenceAnnotation
persistent identity: annotation2414967
display id: annotation2414967
version: 1
top level: BBa_K1321159
location: https://synbiohub.org/public/igem/BBa_K1321159/annotation2414967/range2414967/1
component: BBa_K1319106

https://synbiohub.org/public/igem/BBa_K1321159/annotation2414967/range2414967/1
type: Range
persistent identity: range2414967
display id: range2414967
version: 1
direction: direction/reverse
top level: BBa_K1321159
start: 44
end: 58
orientation: inline

BBa_K1321012
type: SequenceAnnotation
persistent identity: annotation2414969
display id: annotation2414969
version: 1
top level: BBa_K1321159
location: https://synbiohub.org/public/igem/BBa_K1321159/annotation2414969/range2414969/1
component: BBa_K1321012

https://synbiohub.org/public/igem/BBa_K1321159/annotation2414969/range2414969/1
type: Range
persistent identity: range2414969
display id: range2414969
version: 1
direction: direction/reverse
top level: BBa_K1321159
start: 59
end: 256
orientation: inline

BBa_K1689013
type: ComponentDefinition
persistent identity: BBa K1689013
display id: BBa_K1689013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Nlact-dCas9
creator: Jiaofeng Li
created: 2015-09-15T11:00:00Z
modified: 2015-09-27T01:29:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2107_
m user id: 26531
owner id: 26434
owning group id: 9
status: status/Planning
bookmark: false
mutable description: NFluc of lactamase fused with dCas9, His tag allows for protein purification
mutable notes: goldengate
mutable provenance: E.coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: dCas9
sequence annotation: Nlact for N terminal of β-Lactamase
sequence annotation: GGGGS*2 Linker
sequence annotation: stop
sequence annotation: His
sequence annotation: Nlact-dCas9
sequence annotation: start
sequence annotation: random spacer
sequence: https://synbiohub.org/public/igem/BBa_K1689013_sequence/1

dCas9
type: SequenceAnnotation
persistent identity: annotation2470119
display id: annotation2470119
version: 1
top level: BBa_K1689013
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1689013/annotation2470119/range2470119/1

https://synbiohub.org/public/igem/BBa_K1689013/annotation2470119/range2470119/1
type: Range
persistent identity: range2470119
display id: range2470119
version: 1
direction: direction/forward
top level: BBa_K1689013
start: 598
end: 4698
orientation: inline

Nlact for N terminal of β-Lactamase
type: SequenceAnnotation
persistent identity: annotation2470116
display id: annotation2470116
version: 1
top level: BBa_K1689013
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1689013/annotation2470116/range2470116/1

https://synbiohub.org/public/igem/BBa_K1689013/annotation2470116/range2470116/1
type: Range
persistent identity: range2470116
display id: range2470116
version: 1
direction: direction/forward
top level: BBa_K1689013
start: 55
end: 567
orientation: inline

GGGGS*2 Linker
type: SequenceAnnotation
persistent identity: annotation2470117
display id: annotation2470117
version: 1
top level: BBa_K1689013
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1689013/annotation2470117/range2470117/1

https://synbiohub.org/public/igem/BBa_K1689013/annotation2470117/range2470117/1
type: Range
persistent identity: range2470117
display id: range2470117
version: 1
direction: direction/forward
top level: BBa_K1689013
start: 568
end: 697
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2477776
display id: annotation2477776
version: 1
top level: BBa_K1689013
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1689013/annotation2477776/range2477776/1

https://synbiohub.org/public/igem/BBa_K1689013/annotation2477776/range2477776/1
type: Range
persistent identity: range2477776
display id: range2477776
version: 1
direction: direction/forward
top level: BBa_K1689013
start: 4699
end: 4701
orientation: inline

His
type: SequenceAnnotation
persistent identity: annotation2470115
display id: annotation2470115
version: 1
top level: BBa_K1689013
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1689013/annotation2470115/range2470115/1

https://synbiohub.org/public/igem/BBa_K1689013/annotation2470115/range2470115/1
type: Range
persistent identity: range2470115
display id: range2470115
version: 1
direction: direction/forward
top level: BBa_K1689013
start: 37
end: 54
orientation: inline

Nlact-dCas9
type: SequenceAnnotation
persistent identity: annotation2470114
display id: annotation2470114
version: 1
top level: BBa_K1689013
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1689013/annotation2470114/range2470114/1

https://synbiohub.org/public/igem/BBa_K1689013/annotation2470114/range2470114/1
type: Range
persistent identity: range2470114
display id: range2470114
version: 1
direction: direction/forward
top level: BBa_K1689013
start: 34
end: 4701
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2477773
display id: annotation2477773
version: 1
top level: BBa_K1689013
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1689013/annotation2477773/range2477773/1

https://synbiohub.org/public/igem/BBa_K1689013/annotation2477773/range2477773/1
type: Range
persistent identity: range2477773
display id: range2477773
version: 1
direction: direction/forward
top level: BBa_K1689013
start: 34
end: 36
orientation: inline

random spacer
type: SequenceAnnotation
persistent identity: annotation2477775
display id: annotation2477775
version: 1
top level: BBa_K1689013
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1689013/annotation2477775/range2477775/1

https://synbiohub.org/public/igem/BBa_K1689013/annotation2477775/range2477775/1
type: Range
persistent identity: range2477775
display id: range2477775
version: 1
direction: direction/forward
top level: BBa_K1689013
start: 4701
end: 4772
orientation: inline

random spacer
type: SequenceAnnotation
persistent identity: annotation2477774
display id: annotation2477774
version: 1
top level: BBa_K1689013
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1689013/annotation2477774/range2477774/1

https://synbiohub.org/public/igem/BBa_K1689013/annotation2477774/range2477774/1
type: Range
persistent identity: range2477774
display id: range2477774
version: 1
direction: direction/forward
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start: 1
end: 33
orientation: inline

BBa_K1321349
type: ComponentDefinition
persistent identity: BBa K1321349
display id: BBa_K1321349
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CBDcex fused to sfGFP in RFC 25
creator: Chris N Micklem
created: 2014-10-07T11:00:00Z
modified: 2015-06-17T12:30:55Z
discontinued: false
dominant: false
group u list: _1696_
m user id: 4206
owner id: 20830
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: asdf
mutable notes: asdf
mutable provenance: asdf
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1321003
component: BBa_K1321337
component: BBa_B0105
sequence annotation: BBa_K1321003
sequence annotation: BBa_B0105
sequence annotation: BBa_K1321337
sequence: https://synbiohub.org/public/igem/BBa_K1321349_sequence/1

BBa_K1321003
type: Component
persistent identity: component2415149
display id: component2415149
version: 1
top level: BBa_K1321349
access: public
definition: https://synbiohub.org/public/igem/BBa_K1321003/1

BBa_K1321337
type: Component
persistent identity: component2415153
display id: component2415153
version: 1
top level: BBa_K1321349
access: public
definition: BBa_K1321337

BBa_B0105
type: Component
persistent identity: component2415151
display id: component2415151
version: 1
top level: BBa_K1321349
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_K1321003
type: SequenceAnnotation
persistent identity: annotation2415149
display id: annotation2415149
version: 1
top level: BBa_K1321349
location: https://synbiohub.org/public/igem/BBa_K1321349/annotation2415149/range2415149/1
component: BBa_K1321003

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type: Range
persistent identity: range2415149
display id: range2415149
version: 1
direction: direction/reverse
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orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2415151
display id: annotation2415151
version: 1
top level: BBa_K1321349
location: https://synbiohub.org/public/igem/BBa_K1321349/annotation2415151/range2415151/1
component: BBa_B0105

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type: Range
persistent identity: range2415151
display id: range2415151
version: 1
direction: direction/reverse
top level: BBa_K1321349
start: 328
end: 333
orientation: inline

BBa_K1321337
type: SequenceAnnotation
persistent identity: annotation2415153
display id: annotation2415153
version: 1
top level: BBa_K1321349
location: https://synbiohub.org/public/igem/BBa_K1321349/annotation2415153/range2415153/1
component: BBa_K1321337

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type: Range
persistent identity: range2415153
display id: range2415153
version: 1
direction: direction/reverse
top level: BBa_K1321349
start: 334
end: 1044
orientation: inline

BBa_K1692004
type: ComponentDefinition
persistent identity: BBa K1692004
display id: BBa_K1692004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: codon optimized PAL with T7 promoter and Flag Tag
creator: Daniel Kunin
created: 2015-08-02T11:00:00Z
modified: 2015-08-03T01:17:39Z
discontinued: false
dominant: false
group u list: _2110_
m user id: 26209
owner id: 26209
owning group id: 9
status: status/Planning
bookmark: false
mutable description: codon optimized PAL with T7 promoter and Flag Tag
mutable notes: None
mutable provenance: IDT
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1692004_sequence/1

BBa_K1758330
type: ComponentDefinition
persistent identity: BBa K1758330
display id: BBa_K1758330
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lead repressor under control of constitutive promoter and strong RBS
creator: Team Bielefeld-CeBiTec 2015
created: 2015-08-29T11:00:00Z
modified: 2016-01-25T11:24:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2180_
m user id: 4206
owner id: 26630
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Lead promoter repressor under the control of konstitutive promoter (K608002)
mutable notes: ...
mutable provenance: ...
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: PbrR
sequence annotation: K608002
sequence: https://synbiohub.org/public/igem/BBa_K1758330_sequence/1

PbrR
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persistent identity: annotation2440079
display id: annotation2440079
version: 1
top level: BBa_K1758330
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1758330/annotation2440079/range2440079/1

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type: Range
persistent identity: range2440079
display id: range2440079
version: 1
direction: direction/reverse
top level: BBa_K1758330
start: 1
end: 438
orientation: inline

K608002
type: SequenceAnnotation
persistent identity: annotation2440078
display id: annotation2440078
version: 1
top level: BBa_K1758330
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1758330/annotation2440078/range2440078/1

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type: Range
persistent identity: range2440078
display id: range2440078
version: 1
direction: direction/reverse
top level: BBa_K1758330
start: 444
end: 499
orientation: inline

BBa_K1689016
type: ComponentDefinition
persistent identity: BBa K1689016
display id: BBa_K1689016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: dCas9-F[1,2]
creator: Wei weijia
created: 2015-09-15T11:00:00Z
modified: 2015-09-27T01:35:06Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2107_
m user id: 26531
owner id: 26531
owning group id: 9
status: status/Planning
bookmark: false
mutable description: pSB1C3-6His-dCas9-F[1,2], this part is a fusion protein of dCas9-F[1,2], the His tag is used for protein purification
mutable notes: dCas9 fusion protein coding sequence is too long, so you'd better amplify it separately.
mutable provenance: E.coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: F[1,2]-dCas9
sequence annotation: stop
sequence annotation: F[1,2]
sequence annotation: dCas9
sequence annotation: His tag
sequence annotation: GGGGS*2 Linker
sequence annotation: start
sequence annotation: random spacer
sequence: https://synbiohub.org/public/igem/BBa_K1689016_sequence/1

F[1,2]-dCas9
type: SequenceAnnotation
persistent identity: annotation2469957
display id: annotation2469957
version: 1
top level: BBa_K1689016
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1689016/annotation2469957/range2469957/1

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display id: range2469957
version: 1
direction: direction/forward
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start: 34
end: 4449
orientation: inline

stop
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role: SO:0000319
role: feature/stop
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display id: range2477787
version: 1
direction: direction/forward
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start: 4447
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orientation: inline

F[1,2]
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display id: annotation2469962
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role: SO:0000316
role: feature/protein
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persistent identity: range2469962
display id: range2469962
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direction: direction/forward
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start: 4186
end: 4446
orientation: inline

dCas9
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persistent identity: annotation2469959
display id: annotation2469959
version: 1
top level: BBa_K1689016
role: SO:0000316
role: feature/protein
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type: Range
persistent identity: range2469959
display id: range2469959
version: 1
direction: direction/forward
top level: BBa_K1689016
start: 55
end: 4155
orientation: inline

His tag
type: SequenceAnnotation
persistent identity: annotation2469958
display id: annotation2469958
version: 1
top level: BBa_K1689016
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1689016/annotation2469958/range2469958/1

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type: Range
persistent identity: range2469958
display id: range2469958
version: 1
direction: direction/forward
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start: 37
end: 54
orientation: inline

GGGGS*2 Linker
type: SequenceAnnotation
persistent identity: annotation2469960
display id: annotation2469960
version: 1
top level: BBa_K1689016
role: SO:0000316
role: feature/protein
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type: Range
persistent identity: range2469960
display id: range2469960
version: 1
direction: direction/forward
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start: 4156
end: 4185
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2477786
display id: annotation2477786
version: 1
top level: BBa_K1689016
role: SO:0000318
role: feature/start
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type: Range
persistent identity: range2477786
display id: range2477786
version: 1
direction: direction/forward
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start: 34
end: 36
orientation: inline

random spacer
type: SequenceAnnotation
persistent identity: annotation2477785
display id: annotation2477785
version: 1
top level: BBa_K1689016
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1689016/annotation2477785/range2477785/1

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type: Range
persistent identity: range2477785
display id: range2477785
version: 1
direction: direction/forward
top level: BBa_K1689016
start: 1
end: 33
orientation: inline

random spacer
type: SequenceAnnotation
persistent identity: annotation2477788
display id: annotation2477788
version: 1
top level: BBa_K1689016
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1689016/annotation2477788/range2477788/1

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type: Range
persistent identity: range2477788
display id: range2477788
version: 1
direction: direction/forward
top level: BBa_K1689016
start: 4450
end: 4520
orientation: inline

BBa_K1632006
type: ComponentDefinition
persistent identity: BBa K1632006
display id: BBa_K1632006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: fim switch[default ON](Tokyo_Tech/R0010)
creator: Riku Shinohara
created: 2015-08-29T11:00:00Z
modified: 2015-09-18T07:51:53Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2049_
m user id: 24760
owner id: 22687
owning group id: 9
status: status/Planning
bookmark: false
mutable description: a
mutable notes: a
mutable provenance: a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K1632006_sequence/1

1NpuE(C*)
type: ComponentDefinition
persistent identity: BBa K1362103
display id: BBa_K1362103
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS + NpuDnaE C-intein RFC[105] non-slicing control construct (with His6)
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B??scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch??fer, Carolin Schmelas, Silvan Schmitz, Max Waldha
created: 2014-10-07T11:00:00Z
modified: 2015-05-08T01:10:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1738_
m user id: 0
owner id: 12377
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: ???
mutable notes: ???
mutable provenance: ???
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1362418
component: BBa_K1362422
component: BBa_K1362399
component: BBa_K1362421
component: BBa_K1362464
component: BBa_K1362420
component: BBa_K1362090
component: BBa_K1362431
component: BBa_G0000
component: BBa_K1362414
component: BBa_K1362465
sequence annotation: BBa_K1362421
sequence annotation: BBa_K1362422
sequence annotation: BBa_G0000
sequence annotation: BBa_K1362418
sequence annotation: BBa_K1362465
sequence annotation: BBa_K1362464
sequence annotation: BBa_K1362414
sequence annotation: BBa_K1362420
sequence annotation: BBa_K1362090
sequence annotation: BBa_K1362431
sequence annotation: BBa_K1362399
sequence: https://synbiohub.org/public/igem/BBa_K1362103_sequence/1

BBa_K1362418
type: Component
persistent identity: component2411567
display id: component2411567
version: 1
top level: 1NpuE(C*)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362418/1

BBa_K1362422
type: Component
persistent identity: component2411594
display id: component2411594
version: 1
top level: 1NpuE(C*)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362422/1

BBa_K1362399
type: Component
persistent identity: component2411572
display id: component2411572
version: 1
top level: 1NpuE(C*)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362399/1

BBa_K1362421
type: Component
persistent identity: component2411596
display id: component2411596
version: 1
top level: 1NpuE(C*)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362421/1

BBa_K1362464
type: Component
persistent identity: component2411569
display id: component2411569
version: 1
top level: 1NpuE(C*)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362464/1

BBa_K1362420
type: Component
persistent identity: component2411573
display id: component2411573
version: 1
top level: 1NpuE(C*)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362420/1

BBa_K1362090
type: Component
persistent identity: component2411561
display id: component2411561
version: 1
top level: 1NpuE(C*)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362090/1

BBa_K1362431
type: Component
persistent identity: component2411566
display id: component2411566
version: 1
top level: 1NpuE(C*)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362431/1

BBa_G0000
type: Component
persistent identity: component2411562
display id: component2411562
version: 1
top level: 1NpuE(C*)
access: public
definition: https://synbiohub.org/public/igem/BBa_G0000/1

BBa_K1362414
type: Component
persistent identity: component2411563
display id: component2411563
version: 1
top level: 1NpuE(C*)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362414/1

BBa_K1362465
type: Component
persistent identity: component2411597
display id: component2411597
version: 1
top level: 1NpuE(C*)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362465/1

BBa_K1362421
type: SequenceAnnotation
persistent identity: annotation2411596
display id: annotation2411596
version: 1
top level: 1NpuE(C*)
location: https://synbiohub.org/public/igem/BBa_K1362103/annotation2411596/range2411596/1
component: BBa_K1362421

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type: Range
persistent identity: range2411596
display id: range2411596
version: 1
direction: direction/reverse
top level: 1NpuE(C*)
start: 1254
end: 1257
orientation: inline

BBa_K1362422
type: SequenceAnnotation
persistent identity: annotation2411594
display id: annotation2411594
version: 1
top level: 1NpuE(C*)
location: https://synbiohub.org/public/igem/BBa_K1362103/annotation2411594/range2411594/1
component: BBa_K1362422

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type: Range
persistent identity: range2411594
display id: range2411594
version: 1
direction: direction/reverse
top level: 1NpuE(C*)
start: 171
end: 1253
orientation: inline

BBa_G0000
type: SequenceAnnotation
persistent identity: annotation2411562
display id: annotation2411562
version: 1
top level: 1NpuE(C*)
location: https://synbiohub.org/public/igem/BBa_K1362103/annotation2411562/range2411562/1
component: BBa_G0000

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type: Range
persistent identity: range2411562
display id: range2411562
version: 1
direction: direction/reverse
top level: 1NpuE(C*)
start: 30
end: 35
orientation: inline

BBa_K1362418
type: SequenceAnnotation
persistent identity: annotation2411567
display id: annotation2411567
version: 1
top level: 1NpuE(C*)
location: https://synbiohub.org/public/igem/BBa_K1362103/annotation2411567/range2411567/1
component: BBa_K1362418

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type: Range
persistent identity: range2411567
display id: range2411567
version: 1
direction: direction/reverse
top level: 1NpuE(C*)
start: 60
end: 63
orientation: inline

BBa_K1362465
type: SequenceAnnotation
persistent identity: annotation2411597
display id: annotation2411597
version: 1
top level: 1NpuE(C*)
location: https://synbiohub.org/public/igem/BBa_K1362103/annotation2411597/range2411597/1
component: BBa_K1362465

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type: Range
persistent identity: range2411597
display id: range2411597
version: 1
direction: direction/reverse
top level: 1NpuE(C*)
start: 1258
end: 1259
orientation: inline

BBa_K1362464
type: SequenceAnnotation
persistent identity: annotation2411569
display id: annotation2411569
version: 1
top level: 1NpuE(C*)
location: https://synbiohub.org/public/igem/BBa_K1362103/annotation2411569/range2411569/1
component: BBa_K1362464

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type: Range
persistent identity: range2411569
display id: range2411569
version: 1
direction: direction/reverse
top level: 1NpuE(C*)
start: 64
end: 65
orientation: inline

BBa_K1362414
type: SequenceAnnotation
persistent identity: annotation2411563
display id: annotation2411563
version: 1
top level: 1NpuE(C*)
location: https://synbiohub.org/public/igem/BBa_K1362103/annotation2411563/range2411563/1
component: BBa_K1362414

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type: Range
persistent identity: range2411563
display id: range2411563
version: 1
direction: direction/reverse
top level: 1NpuE(C*)
start: 36
end: 38
orientation: inline

BBa_K1362420
type: SequenceAnnotation
persistent identity: annotation2411573
display id: annotation2411573
version: 1
top level: 1NpuE(C*)
location: https://synbiohub.org/public/igem/BBa_K1362103/annotation2411573/range2411573/1
component: BBa_K1362420

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type: Range
persistent identity: range2411573
display id: range2411573
version: 1
direction: direction/reverse
top level: 1NpuE(C*)
start: 167
end: 170
orientation: inline

BBa_K1362090
type: SequenceAnnotation
persistent identity: annotation2411561
display id: annotation2411561
version: 1
top level: 1NpuE(C*)
location: https://synbiohub.org/public/igem/BBa_K1362103/annotation2411561/range2411561/1
component: BBa_K1362090

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type: Range
persistent identity: range2411561
display id: range2411561
version: 1
direction: direction/reverse
top level: 1NpuE(C*)
start: 1
end: 29
orientation: inline

BBa_K1362431
type: SequenceAnnotation
persistent identity: annotation2411566
display id: annotation2411566
version: 1
top level: 1NpuE(C*)
location: https://synbiohub.org/public/igem/BBa_K1362103/annotation2411566/range2411566/1
component: BBa_K1362431

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type: Range
persistent identity: range2411566
display id: range2411566
version: 1
direction: direction/reverse
top level: 1NpuE(C*)
start: 39
end: 59
orientation: inline

BBa_K1362399
type: SequenceAnnotation
persistent identity: annotation2411572
display id: annotation2411572
version: 1
top level: 1NpuE(C*)
location: https://synbiohub.org/public/igem/BBa_K1362103/annotation2411572/range2411572/1
component: BBa_K1362399

https://synbiohub.org/public/igem/BBa_K1362103/annotation2411572/range2411572/1
type: Range
persistent identity: range2411572
display id: range2411572
version: 1
direction: direction/reverse
top level: 1NpuE(C*)
start: 66
end: 166
orientation: inline

BBa_K1602043
type: ComponentDefinition
persistent identity: BBa K1602043
display id: BBa_K1602043
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: araCpBad-hokD
creator: Christian Sator,Stefan Zens, Max Zander, Benedikt Spannenkrebs, Sebastian Jaeger
created: 2015-09-15T11:00:00Z
modified: 2015-09-25T05:19:21Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2019_
m user id: 23028
owner id: 26220
owning group id: 9
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K808000
component: BBa_B0034
component: BBa_K1497008
sequence annotation: BBa_B0034
sequence annotation: BBa_K808000
sequence annotation: BBa_K1497008
sequence: https://synbiohub.org/public/igem/BBa_K1602043_sequence/1

BBa_K808000
type: Component
persistent identity: component2462889
display id: component2462889
version: 1
top level: BBa_K1602043
access: public
definition: https://synbiohub.org/public/igem/BBa_K808000/1

BBa_B0034
type: Component
persistent identity: component2462891
display id: component2462891
version: 1
top level: BBa_K1602043
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1497008
type: Component
persistent identity: component2462892
display id: component2462892
version: 1
top level: BBa_K1602043
access: public
definition: BBa_K1497008

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2462891
display id: annotation2462891
version: 1
top level: BBa_K1602043
location: https://synbiohub.org/public/igem/BBa_K1602043/annotation2462891/range2462891/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1602043/annotation2462891/range2462891/1
type: Range
persistent identity: range2462891
display id: range2462891
version: 1
direction: direction/reverse
top level: BBa_K1602043
start: 1218
end: 1229
orientation: inline

BBa_K808000
type: SequenceAnnotation
persistent identity: annotation2462889
display id: annotation2462889
version: 1
top level: BBa_K1602043
location: https://synbiohub.org/public/igem/BBa_K1602043/annotation2462889/range2462889/1
component: BBa_K808000

https://synbiohub.org/public/igem/BBa_K1602043/annotation2462889/range2462889/1
type: Range
persistent identity: range2462889
display id: range2462889
version: 1
direction: direction/reverse
top level: BBa_K1602043
start: 1
end: 1209
orientation: inline

BBa_K1497008
type: SequenceAnnotation
persistent identity: annotation2462892
display id: annotation2462892
version: 1
top level: BBa_K1602043
location: https://synbiohub.org/public/igem/BBa_K1602043/annotation2462892/range2462892/1
component: BBa_K1497008

https://synbiohub.org/public/igem/BBa_K1602043/annotation2462892/range2462892/1
type: Range
persistent identity: range2462892
display id: range2462892
version: 1
direction: direction/reverse
top level: BBa_K1602043
start: 1236
end: 1391
orientation: inline

BBa_K1758376
type: ComponentDefinition
persistent identity: BBa K1758376
display id: BBa_K1758376
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GHB / GBL biosensor - reporter part
creator: Team Bielefeld-CeBiTec 2015
created: 2015-08-31T11:00:00Z
modified: 2015-09-19T01:22:36Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2180_
m user id: 26563
owner id: 26563
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This part contains all features from [http://parts.igem.org/Part:BBa_K1758102 BBa_K1758102] plus the blc-operator site described in [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4083662/ Pan et al. 2013]. BlcR (see http://parts.igem.org/Part:BBa_K1758370 K1758370]) can bind to the operator site.
mutable notes: ---
mutable provenance: This part was assembled via PCR with Gibson primers on [http://parts.igem.org/Part:BBa_K1758102 BBa_K1758102]
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: 5' -UTR
sequence annotation: B0010
sequence annotation: silent mutation
sequence annotation: K1758101
sequence annotation: B0012
sequence annotation: sfGFP
sequence annotation: K1758100
sequence annotation: T7 promoter
sequence annotation: Blc operator
sequence: https://synbiohub.org/public/igem/BBa_K1758376_sequence/1

5' -UTR
type: SequenceAnnotation
persistent identity: annotation2441679
display id: annotation2441679
version: 1
top level: BBa_K1758376
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1758376/annotation2441679/range2441679/1

https://synbiohub.org/public/igem/BBa_K1758376/annotation2441679/range2441679/1
type: Range
persistent identity: range2441679
display id: range2441679
version: 1
direction: direction/forward
top level: BBa_K1758376
start: 64
end: 109
orientation: inline

B0010
type: SequenceAnnotation
persistent identity: annotation2441683
display id: annotation2441683
version: 1
top level: BBa_K1758376
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1758376/annotation2441683/range2441683/1

https://synbiohub.org/public/igem/BBa_K1758376/annotation2441683/range2441683/1
type: Range
persistent identity: range2441683
display id: range2441683
version: 1
direction: direction/forward
top level: BBa_K1758376
start: 838
end: 917
orientation: inline

silent mutation
type: SequenceAnnotation
persistent identity: annotation2441694
display id: annotation2441694
version: 1
top level: BBa_K1758376
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K1758376/annotation2441694/range2441694/1

https://synbiohub.org/public/igem/BBa_K1758376/annotation2441694/range2441694/1
type: Range
persistent identity: range2441694
display id: range2441694
version: 1
direction: direction/forward
top level: BBa_K1758376
start: 691
end: 691
orientation: inline

K1758101
type: SequenceAnnotation
persistent identity: annotation2441681
display id: annotation2441681
version: 1
top level: BBa_K1758376
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1758376/annotation2441681/range2441681/1

https://synbiohub.org/public/igem/BBa_K1758376/annotation2441681/range2441681/1
type: Range
persistent identity: range2441681
display id: range2441681
version: 1
direction: direction/forward
top level: BBa_K1758376
start: 64
end: 966
orientation: inline

B0012
type: SequenceAnnotation
persistent identity: annotation2441684
display id: annotation2441684
version: 1
top level: BBa_K1758376
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1758376/annotation2441684/range2441684/1

https://synbiohub.org/public/igem/BBa_K1758376/annotation2441684/range2441684/1
type: Range
persistent identity: range2441684
display id: range2441684
version: 1
direction: direction/forward
top level: BBa_K1758376
start: 926
end: 966
orientation: inline

sfGFP
type: SequenceAnnotation
persistent identity: annotation2441682
display id: annotation2441682
version: 1
top level: BBa_K1758376
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1758376/annotation2441682/range2441682/1

https://synbiohub.org/public/igem/BBa_K1758376/annotation2441682/range2441682/1
type: Range
persistent identity: range2441682
display id: range2441682
version: 1
direction: direction/forward
top level: BBa_K1758376
start: 110
end: 829
orientation: inline

K1758100
type: SequenceAnnotation
persistent identity: annotation2441680
display id: annotation2441680
version: 1
top level: BBa_K1758376
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1758376/annotation2441680/range2441680/1

https://synbiohub.org/public/igem/BBa_K1758376/annotation2441680/range2441680/1
type: Range
persistent identity: range2441680
display id: range2441680
version: 1
direction: direction/forward
top level: BBa_K1758376
start: 64
end: 109
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2441677
display id: annotation2441677
version: 1
top level: BBa_K1758376
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1758376/annotation2441677/range2441677/1

https://synbiohub.org/public/igem/BBa_K1758376/annotation2441677/range2441677/1
type: Range
persistent identity: range2441677
display id: range2441677
version: 1
direction: direction/forward
top level: BBa_K1758376
start: 1
end: 22
orientation: inline

Blc operator
type: SequenceAnnotation
persistent identity: annotation2441678
display id: annotation2441678
version: 1
top level: BBa_K1758376
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K1758376/annotation2441678/range2441678/1

https://synbiohub.org/public/igem/BBa_K1758376/annotation2441678/range2441678/1
type: Range
persistent identity: range2441678
display id: range2441678
version: 1
direction: direction/forward
top level: BBa_K1758376
start: 23
end: 63
orientation: inline

BBa_K1763442
type: ComponentDefinition
persistent identity: BBa K1763442
display id: BBa_K1763442
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: MaSp1/2[1:2]-12(1C3)
creator: Vinson Lam
created: 2015-09-14T11:00:00Z
modified: 2015-09-17T12:06:58Z
discontinued: false
dominant: false
group u list: _2185_
m user id: 27136
owner id: 27136
owning group id: 9
status: status/Planning
bookmark: false
mutable description: MaSp1 and MaSp2 12-mer block co-polymer in pSB1C3, with no regulatory elements. Ratio of 1 MaSp1 to 2 MaSp2.
mutable notes: no
mutable provenance: N. clavipes
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: M1-CA
sequence annotation: M2-BC
sequence annotation: M1-BC
sequence annotation: M1-AB
sequence annotation: M2-CA
sequence annotation: M2-AB
sequence annotation: 8-his tag
sequence: https://synbiohub.org/public/igem/BBa_K1763442_sequence/1

M1-CA
type: SequenceAnnotation
persistent identity: annotation2464153
display id: annotation2464153
version: 1
top level: BBa_K1763442
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763442/annotation2464153/range2464153/1

https://synbiohub.org/public/igem/BBa_K1763442/annotation2464153/range2464153/1
type: Range
persistent identity: range2464153
display id: range2464153
version: 1
direction: direction/forward
top level: BBa_K1763442
start: 240
end: 341
orientation: inline

M2-BC
type: SequenceAnnotation
persistent identity: annotation2464147
display id: annotation2464147
version: 1
top level: BBa_K1763442
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763442/annotation2464147/range2464147/1

https://synbiohub.org/public/igem/BBa_K1763442/annotation2464147/range2464147/1
type: Range
persistent identity: range2464147
display id: range2464147
version: 1
direction: direction/forward
top level: BBa_K1763442
start: 1056
end: 1157
orientation: inline

M1-BC
type: SequenceAnnotation
persistent identity: annotation2464149
display id: annotation2464149
version: 1
top level: BBa_K1763442
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763442/annotation2464149/range2464149/1

https://synbiohub.org/public/igem/BBa_K1763442/annotation2464149/range2464149/1
type: Range
persistent identity: range2464149
display id: range2464149
version: 1
direction: direction/forward
top level: BBa_K1763442
start: 138
end: 239
orientation: inline

M1-AB
type: SequenceAnnotation
persistent identity: annotation2464152
display id: annotation2464152
version: 1
top level: BBa_K1763442
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763442/annotation2464152/range2464152/1

https://synbiohub.org/public/igem/BBa_K1763442/annotation2464152/range2464152/1
type: Range
persistent identity: range2464152
display id: range2464152
version: 1
direction: direction/forward
top level: BBa_K1763442
start: 342
end: 443
orientation: inline

M2-BC
type: SequenceAnnotation
persistent identity: annotation2464144
display id: annotation2464144
version: 1
top level: BBa_K1763442
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763442/annotation2464144/range2464144/1

https://synbiohub.org/public/igem/BBa_K1763442/annotation2464144/range2464144/1
type: Range
persistent identity: range2464144
display id: range2464144
version: 1
direction: direction/forward
top level: BBa_K1763442
start: 444
end: 545
orientation: inline

M2-CA
type: SequenceAnnotation
persistent identity: annotation2464145
display id: annotation2464145
version: 1
top level: BBa_K1763442
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763442/annotation2464145/range2464145/1

https://synbiohub.org/public/igem/BBa_K1763442/annotation2464145/range2464145/1
type: Range
persistent identity: range2464145
display id: range2464145
version: 1
direction: direction/forward
top level: BBa_K1763442
start: 852
end: 953
orientation: inline

M2-AB
type: SequenceAnnotation
persistent identity: annotation2464151
display id: annotation2464151
version: 1
top level: BBa_K1763442
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763442/annotation2464151/range2464151/1

https://synbiohub.org/public/igem/BBa_K1763442/annotation2464151/range2464151/1
type: Range
persistent identity: range2464151
display id: range2464151
version: 1
direction: direction/forward
top level: BBa_K1763442
start: 648
end: 749
orientation: inline

M2-AB
type: SequenceAnnotation
persistent identity: annotation2464148
display id: annotation2464148
version: 1
top level: BBa_K1763442
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763442/annotation2464148/range2464148/1

https://synbiohub.org/public/igem/BBa_K1763442/annotation2464148/range2464148/1
type: Range
persistent identity: range2464148
display id: range2464148
version: 1
direction: direction/forward
top level: BBa_K1763442
start: 954
end: 1055
orientation: inline

M2-BC
type: SequenceAnnotation
persistent identity: annotation2464150
display id: annotation2464150
version: 1
top level: BBa_K1763442
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763442/annotation2464150/range2464150/1

https://synbiohub.org/public/igem/BBa_K1763442/annotation2464150/range2464150/1
type: Range
persistent identity: range2464150
display id: range2464150
version: 1
direction: direction/forward
top level: BBa_K1763442
start: 750
end: 851
orientation: inline

8-his tag
type: SequenceAnnotation
persistent identity: annotation2464143
display id: annotation2464143
version: 1
top level: BBa_K1763442
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1763442/annotation2464143/range2464143/1

https://synbiohub.org/public/igem/BBa_K1763442/annotation2464143/range2464143/1
type: Range
persistent identity: range2464143
display id: range2464143
version: 1
direction: direction/forward
top level: BBa_K1763442
start: 4
end: 27
orientation: inline

M1-AB
type: SequenceAnnotation
persistent identity: annotation2464155
display id: annotation2464155
version: 1
top level: BBa_K1763442
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763442/annotation2464155/range2464155/1

https://synbiohub.org/public/igem/BBa_K1763442/annotation2464155/range2464155/1
type: Range
persistent identity: range2464155
display id: range2464155
version: 1
direction: direction/forward
top level: BBa_K1763442
start: 36
end: 137
orientation: inline

M2-CA
type: SequenceAnnotation
persistent identity: annotation2464154
display id: annotation2464154
version: 1
top level: BBa_K1763442
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763442/annotation2464154/range2464154/1

https://synbiohub.org/public/igem/BBa_K1763442/annotation2464154/range2464154/1
type: Range
persistent identity: range2464154
display id: range2464154
version: 1
direction: direction/forward
top level: BBa_K1763442
start: 546
end: 647
orientation: inline

M2-CA
type: SequenceAnnotation
persistent identity: annotation2464146
display id: annotation2464146
version: 1
top level: BBa_K1763442
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763442/annotation2464146/range2464146/1

https://synbiohub.org/public/igem/BBa_K1763442/annotation2464146/range2464146/1
type: Range
persistent identity: range2464146
display id: range2464146
version: 1
direction: direction/forward
top level: BBa_K1763442
start: 1158
end: 1259
orientation: inline

pBad/araC
type: ComponentDefinition
persistent identity: BBa I0500
display id: BBa_I0500
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Inducible pBad/araC promoter
creator: Sri Kosuri
created: 2003-12-05T12:00:00Z
modified: 2015-08-31T04:07:29Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: PC
sequence annotation: AraC
sequence annotation: Pbad
sequence: https://synbiohub.org/public/igem/BBa_I0500_sequence/1

PC
type: SequenceAnnotation
persistent identity: annotation1498842
display id: annotation1498842
version: 1
top level: pBad/araC
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I0500/annotation1498842/range1498842/1

https://synbiohub.org/public/igem/BBa_I0500/annotation1498842/range1498842/1
type: Range
persistent identity: range1498842
display id: range1498842
version: 1
direction: direction/forward
top level: pBad/araC
start: 1043
end: 1114
orientation: inline

AraC
type: SequenceAnnotation
persistent identity: annotation1498843
display id: annotation1498843
version: 1
top level: pBad/araC
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I0500/annotation1498843/range1498843/1

https://synbiohub.org/public/igem/BBa_I0500/annotation1498843/range1498843/1
type: Range
persistent identity: range1498843
display id: range1498843
version: 1
direction: direction/forward
top level: pBad/araC
start: 1
end: 1079
orientation: inline

Pbad
type: SequenceAnnotation
persistent identity: annotation1498841
display id: annotation1498841
version: 1
top level: pBad/araC
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I0500/annotation1498841/range1498841/1

https://synbiohub.org/public/igem/BBa_I0500/annotation1498841/range1498841/1
type: Range
persistent identity: range1498841
display id: range1498841
version: 1
direction: direction/forward
top level: pBad/araC
start: 1080
end: 1210
orientation: inline

BBa_K1763015
type: ComponentDefinition
persistent identity: BBa K1763015
display id: BBa_K1763015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 Promoter+Silk+sfGFP
creator: Megan Satyadi
created: 2015-08-21T11:00:00Z
modified: 2016-02-10T11:25:59Z
discontinued: false
dominant: false
group u list: _2185_
m user id: 4206
owner id: 27400
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This part is a fusion of the Apis mellifera (Western Honey Bee) silk protein coding region and the superfolder GFP coding region. A T7 promoter regulatory element has been added upstream, along with a ribosomal binding site. (in progress)
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence: https://synbiohub.org/public/igem/BBa_K1763015_sequence/1

BBa_K1497008
type: ComponentDefinition
persistent identity: BBa K1497008
display id: BBa_K1497008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: hokD (relF)
creator: Sascha Hein, Daniel Sachs, Christian Sator
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1877_
m user id: 0
owner id: 12466
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: d
mutable notes: d
mutable provenance: d
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1497008_sequence/1

BBa_K1321345
type: ComponentDefinition
persistent identity: BBa K1321345
display id: BBa_K1321345
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NiBP fused to dCBD with linker in RFC 25
creator: Chris N Micklem
created: 2014-10-07T11:00:00Z
modified: 2015-06-17T12:25:54Z
discontinued: false
dominant: false
group u list: _1696_
m user id: 4206
owner id: 20830
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: asdf
mutable notes: asdf
mutable provenance: asdf
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1321340
component: BBa_K1321009
component: BBa_B0105
sequence annotation: BBa_K1321009
sequence annotation: BBa_K1321340
sequence annotation: BBa_B0105
sequence: https://synbiohub.org/public/igem/BBa_K1321345_sequence/1

BBa_K1321340
type: Component
persistent identity: component2415114
display id: component2415114
version: 1
top level: BBa_K1321345
access: public
definition: BBa_K1321340

BBa_K1321009
type: Component
persistent identity: component2415110
display id: component2415110
version: 1
top level: BBa_K1321345
access: public
definition: BBa_K1321009

BBa_B0105
type: Component
persistent identity: component2415112
display id: component2415112
version: 1
top level: BBa_K1321345
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_K1321009
type: SequenceAnnotation
persistent identity: annotation2415110
display id: annotation2415110
version: 1
top level: BBa_K1321345
location: https://synbiohub.org/public/igem/BBa_K1321345/annotation2415110/range2415110/1
component: BBa_K1321009

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type: Range
persistent identity: range2415110
display id: range2415110
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direction: direction/reverse
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start: 1
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orientation: inline

BBa_K1321340
type: SequenceAnnotation
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component: BBa_K1321340

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BBa_B0105
type: SequenceAnnotation
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display id: annotation2415112
version: 1
top level: BBa_K1321345
location: https://synbiohub.org/public/igem/BBa_K1321345/annotation2415112/range2415112/1
component: BBa_B0105

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type: Range
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display id: range2415112
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end: 182
orientation: inline

BBa_K1399010
type: ComponentDefinition
persistent identity: BBa K1399010
display id: BBa_K1399010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Plac-RFP(AAV)
creator: Anna Stikane
created: 2014-09-18T11:00:00Z
modified: 2015-05-08T01:10:16Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1777_
m user id: 0
owner id: 22477
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: Lactose/IPTG inducible promoter wuth RFP reporter tagged with AAV-ssrA degradation tag followed by terminator. The tagged RFP is actively degraded within cell, thus provides better temporal resolution of red fluorescence and promoter activity. Part was assembled using BrickClip assembly (BBF RFC 104) using other biobrick parts as templates. BrickClip assembly is a special case of more general Paperclip assembly method.[1] Reference [1] Trubitsyna, M., Michlewski, G., Cai, Y., Elfick, A. & French, C. E. PaperClip: rapid multi-part DNA assembly from existing libraries. Nucleic Acids Res. 1???8 (2014). doi:10.1093/nar/gku829
mutable notes: no
mutable provenance: Other biobrick parts
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: conserved
sequence annotation: start
sequence annotation: T7 TE
sequence annotation: end of LacI coding region (inactive)
sequence annotation: stop
sequence annotation: AAV-ssrA tag
sequence annotation: BBa_B0015
sequence annotation: BBa_B0012
sequence annotation: polya
sequence annotation: BBa_B0010
sequence annotation: BBa_E1010 w/o stop
sequence annotation: BBa_R0010
sequence annotation: barcode from BBa_E1010
sequence annotation: LacI binding site
sequence annotation: BBa_B0034
sequence annotation: CAP binding site
sequence annotation: BBa_K1399000
sequence annotation: -10
sequence annotation: -35
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_K1399010_sequence/1

conserved
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start: 213
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start
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end: 173
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T7 TE
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display id: annotation2383951
version: 1
top level: BBa_K1399010
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1399010/annotation2383951/range2383951/1

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type: Range
persistent identity: range2383951
display id: range2383951
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direction: direction/forward
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start: 1069
end: 1088
orientation: inline

end of LacI coding region (inactive)
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persistent identity: annotation2383934
display id: annotation2383934
version: 1
top level: BBa_K1399010
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1399010/annotation2383934/range2383934/1

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display id: range2383934
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start: 1
end: 88
orientation: inline

stop
type: SequenceAnnotation
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role: SO:0000319
role: feature/stop
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type: Range
persistent identity: range2383945
display id: range2383945
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top level: BBa_K1399010
start: 935
end: 937
orientation: inline

stop
type: SequenceAnnotation
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top level: BBa_K1399010
role: SO:0000319
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display id: range2383953
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direction: direction/forward
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start: 1095
end: 1095
orientation: inline

AAV-ssrA tag
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persistent identity: annotation2383944
display id: annotation2383944
version: 1
top level: BBa_K1399010
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1399010/annotation2383944/range2383944/1

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persistent identity: range2383944
display id: range2383944
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direction: direction/forward
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start: 902
end: 934
orientation: inline

BBa_B0015
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top level: BBa_K1399010
role: SO:0000804
role: feature/BioBrick
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direction: direction/reverse
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orientation: inline

BBa_B0012
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version: 1
top level: BBa_K1399010
role: SO:0000804
role: feature/BioBrick
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persistent identity: range2383950
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polya
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BBa_B0010
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role: SO:0000804
role: feature/BioBrick
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BBa_E1010 w/o stop
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top level: BBa_K1399010
role: feature/cds
role: SO:0000316
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direction: direction/forward
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start: 230
end: 901
orientation: inline

BBa_R0010
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display id: annotation2383933
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top level: BBa_K1399010
role: SO:0000804
role: feature/BioBrick
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type: Range
persistent identity: range2383933
display id: range2383933
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direction: direction/forward
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start: 1
end: 200
orientation: inline

stop
type: SequenceAnnotation
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top level: BBa_K1399010
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399010/annotation2383946/range2383946/1

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type: Range
persistent identity: range2383946
display id: range2383946
version: 1
direction: direction/forward
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start: 938
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orientation: inline

barcode from BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2386257
display id: annotation2386257
version: 1
top level: BBa_K1399010
role: SO:0000110
role: feature/misc
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persistent identity: range2386257
display id: range2386257
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end: 965
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation2383938
display id: annotation2383938
version: 1
top level: BBa_K1399010
role: feature/binding
role: SO:0001091
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direction: direction/forward
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start: 166
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orientation: inline

BBa_B0034
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top level: BBa_K1399010
role: SO:0000804
role: feature/BioBrick
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persistent identity: range2383941
display id: range2383941
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direction: direction/reverse
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orientation: inline

CAP binding site
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display id: annotation2383935
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top level: BBa_K1399010
role: feature/binding
role: SO:0001091
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display id: range2383935
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direction: direction/forward
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start: 89
end: 126
orientation: inline

BBa_K1399000
type: SequenceAnnotation
persistent identity: annotation2383947
display id: annotation2383947
version: 1
top level: BBa_K1399010
role: SO:0000804
role: feature/BioBrick
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type: Range
persistent identity: range2383947
display id: range2383947
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direction: direction/reverse
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start: 227
end: 940
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2383937
display id: annotation2383937
version: 1
top level: BBa_K1399010
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1399010/annotation2383937/range2383937/1

https://synbiohub.org/public/igem/BBa_K1399010/annotation2383937/range2383937/1
type: Range
persistent identity: range2383937
display id: range2383937
version: 1
direction: direction/forward
top level: BBa_K1399010
start: 161
end: 166
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2383936
display id: annotation2383936
version: 1
top level: BBa_K1399010
role: SO:0000167
role: feature/promoter
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type: Range
persistent identity: range2383936
display id: range2383936
version: 1
direction: direction/forward
top level: BBa_K1399010
start: 137
end: 142
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation2383949
display id: annotation2383949
version: 1
top level: BBa_K1399010
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1399010/annotation2383949/range2383949/1

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type: Range
persistent identity: range2383949
display id: range2383949
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direction: direction/forward
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start: 985
end: 1028
orientation: inline

start
type: SequenceAnnotation
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display id: annotation2383942
version: 1
top level: BBa_K1399010
role: SO:0000318
role: feature/start
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type: Range
persistent identity: range2383942
display id: range2383942
version: 1
direction: direction/forward
top level: BBa_K1399010
start: 227
end: 229
orientation: inline

BBa_K1696009
type: ComponentDefinition
persistent identity: BBa K1696009
display id: BBa_K1696009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lactate sensing system
creator: Yue Liu
created: 2015-09-13T11:00:00Z
modified: 2015-09-14T07:07:15Z
discontinued: false
dominant: false
group u list: _2114_
m user id: 16881
owner id: 16881
owning group id: 9
status: status/Planning
bookmark: false
mutable description: We construct a lactate sensing system for our MFC.
mutable notes: Keep special enzyme site to change component of the system in order to regulate parts' expression level.
mutable provenance: From Lactococcus lactis.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: P170
sequence annotation: RPF
sequence annotation: RcfB
sequence: https://synbiohub.org/public/igem/BBa_K1696009_sequence/1

P170
type: SequenceAnnotation
persistent identity: annotation2454076
display id: annotation2454076
version: 1
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role: SO:0000167
role: feature/promoter
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persistent identity: range2454076
display id: range2454076
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direction: direction/forward
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start: 1
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orientation: inline

RPF
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persistent identity: annotation2454078
display id: annotation2454078
version: 1
top level: BBa_K1696009
role: SO:0000110
role: feature/misc
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type: Range
persistent identity: range2454078
display id: range2454078
version: 1
direction: direction/forward
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start: 455
end: 1122
orientation: inline

RcfB
type: SequenceAnnotation
persistent identity: annotation2454094
display id: annotation2454094
version: 1
top level: BBa_K1696009
role: feature/cds
role: SO:0000316
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type: Range
persistent identity: range2454094
display id: range2454094
version: 1
direction: direction/forward
top level: BBa_K1696009
start: 1295
end: 1981
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BBa_K1362058
type: ComponentDefinition
persistent identity: BBa K1362058
display id: BBa_K1362058
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PelB leading sequence as RFC[105] N-terminal insert
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B??scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch??fer, Carolin Schmelas, Silvan Schmitz, Max Waldha
created: 2014-10-07T11:00:00Z
modified: 2015-05-08T01:10:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1738_
m user id: 0
owner id: 22830
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000324
role: partType/Tag
component: BBa_K1362415
component: BBa_K1362444
component: BBa_K1362425
component: BBa_K1362423
component: BBa_J32015
sequence annotation: BBa_K1362444
sequence annotation: BBa_K1362425
sequence annotation: BBa_K1362415
sequence annotation: BBa_J32015
sequence annotation: BBa_K1362423
sequence: https://synbiohub.org/public/igem/BBa_K1362058_sequence/1

BBa_K1362415
type: Component
persistent identity: component2409737
display id: component2409737
version: 1
top level: BBa_K1362058
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362415/1

BBa_K1362444
type: Component
persistent identity: component2409736
display id: component2409736
version: 1
top level: BBa_K1362058
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362444/1

BBa_K1362425
type: Component
persistent identity: component2409733
display id: component2409733
version: 1
top level: BBa_K1362058
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362425/1

BBa_K1362423
type: Component
persistent identity: component2409738
display id: component2409738
version: 1
top level: BBa_K1362058
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362423/1

BBa_J32015
type: Component
persistent identity: component2409735
display id: component2409735
version: 1
top level: BBa_K1362058
access: public
definition: https://synbiohub.org/public/igem/BBa_J32015/1

BBa_K1362444
type: SequenceAnnotation
persistent identity: annotation2409736
display id: annotation2409736
version: 1
top level: BBa_K1362058
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component: BBa_K1362444

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type: Range
persistent identity: range2409736
display id: range2409736
version: 1
direction: direction/reverse
top level: BBa_K1362058
start: 74
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orientation: inline

BBa_K1362425
type: SequenceAnnotation
persistent identity: annotation2409733
display id: annotation2409733
version: 1
top level: BBa_K1362058
location: https://synbiohub.org/public/igem/BBa_K1362058/annotation2409733/range2409733/1
component: BBa_K1362425

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type: Range
persistent identity: range2409733
display id: range2409733
version: 1
direction: direction/reverse
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start: 1
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BBa_K1362415
type: SequenceAnnotation
persistent identity: annotation2409737
display id: annotation2409737
version: 1
top level: BBa_K1362058
location: https://synbiohub.org/public/igem/BBa_K1362058/annotation2409737/range2409737/1
component: BBa_K1362415

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type: Range
persistent identity: range2409737
display id: range2409737
version: 1
direction: direction/reverse
top level: BBa_K1362058
start: 92
end: 95
orientation: inline

BBa_J32015
type: SequenceAnnotation
persistent identity: annotation2409735
display id: annotation2409735
version: 1
top level: BBa_K1362058
location: https://synbiohub.org/public/igem/BBa_K1362058/annotation2409735/range2409735/1
component: BBa_J32015

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type: Range
persistent identity: range2409735
display id: range2409735
version: 1
direction: direction/reverse
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start: 8
end: 73
orientation: inline

BBa_K1362423
type: SequenceAnnotation
persistent identity: annotation2409738
display id: annotation2409738
version: 1
top level: BBa_K1362058
location: https://synbiohub.org/public/igem/BBa_K1362058/annotation2409738/range2409738/1
component: BBa_K1362423

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type: Range
persistent identity: range2409738
display id: range2409738
version: 1
direction: direction/reverse
top level: BBa_K1362058
start: 96
end: 102
orientation: inline

sfGFP_T65C
type: ComponentDefinition
persistent identity: BBa K1362170
display id: BBa_K1362170
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Split sfGFP reconstitution positive control
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Buescher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schaefer, Carolin Schmelas, Silvan Schmitz, Max Waldhauer
created: 2014-10-08T11:00:00Z
modified: 2015-05-08T01:10:05Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1738_
m user id: 0
owner id: 12377
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: ???
mutable notes: ???
mutable provenance: ???
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_K1362470
component: BBa_K1362468
component: BBa_G0000
component: BBa_K1362090
component: BBa_J70594
component: BBa_K371056
sequence annotation: BBa_K1362470
sequence annotation: BBa_J70594
sequence annotation: BBa_K371056
sequence annotation: BBa_K1362468
sequence annotation: BBa_K1362090
sequence annotation: BBa_G0000
sequence: https://synbiohub.org/public/igem/BBa_K1362170_sequence/1

BBa_K1362470
type: Component
persistent identity: component2414461
display id: component2414461
version: 1
top level: sfGFP_T65C
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362470/1

BBa_K1362468
type: Component
persistent identity: component2414460
display id: component2414460
version: 1
top level: sfGFP_T65C
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362468/1

BBa_G0000
type: Component
persistent identity: component2414457
display id: component2414457
version: 1
top level: sfGFP_T65C
access: public
definition: https://synbiohub.org/public/igem/BBa_G0000/1

BBa_K1362090
type: Component
persistent identity: component2414456
display id: component2414456
version: 1
top level: sfGFP_T65C
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362090/1

BBa_K1362468
type: Component
persistent identity: component2414462
display id: component2414462
version: 1
top level: sfGFP_T65C
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362468/1

BBa_J70594
type: Component
persistent identity: component2414464
display id: component2414464
version: 1
top level: sfGFP_T65C
access: public
definition: https://synbiohub.org/public/igem/BBa_J70594/1

BBa_K371056
type: Component
persistent identity: component2414459
display id: component2414459
version: 1
top level: sfGFP_T65C
access: public
definition: https://synbiohub.org/public/igem/BBa_K371056/1

BBa_K1362470
type: SequenceAnnotation
persistent identity: annotation2414461
display id: annotation2414461
version: 1
top level: sfGFP_T65C
location: https://synbiohub.org/public/igem/BBa_K1362170/annotation2414461/range2414461/1
component: BBa_K1362470

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type: Range
persistent identity: range2414461
display id: range2414461
version: 1
direction: direction/reverse
top level: sfGFP_T65C
start: 57
end: 767
orientation: inline

BBa_J70594
type: SequenceAnnotation
persistent identity: annotation2414464
display id: annotation2414464
version: 1
top level: sfGFP_T65C
location: https://synbiohub.org/public/igem/BBa_K1362170/annotation2414464/range2414464/1
component: BBa_J70594

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type: Range
persistent identity: range2414464
display id: range2414464
version: 1
direction: direction/reverse
top level: sfGFP_T65C
start: 786
end: 791
orientation: inline

BBa_K371056
type: SequenceAnnotation
persistent identity: annotation2414459
display id: annotation2414459
version: 1
top level: sfGFP_T65C
location: https://synbiohub.org/public/igem/BBa_K1362170/annotation2414459/range2414459/1
component: BBa_K371056

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type: Range
persistent identity: range2414459
display id: range2414459
version: 1
direction: direction/reverse
top level: sfGFP_T65C
start: 36
end: 38
orientation: inline

BBa_K1362468
type: SequenceAnnotation
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display id: annotation2414462
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top level: sfGFP_T65C
location: https://synbiohub.org/public/igem/BBa_K1362170/annotation2414462/range2414462/1
component: BBa_K1362468

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type: Range
persistent identity: range2414462
display id: range2414462
version: 1
direction: direction/reverse
top level: sfGFP_T65C
start: 768
end: 785
orientation: inline

BBa_K1362090
type: SequenceAnnotation
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display id: annotation2414456
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top level: sfGFP_T65C
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component: BBa_K1362090

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type: Range
persistent identity: range2414456
display id: range2414456
version: 1
direction: direction/reverse
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start: 1
end: 29
orientation: inline

BBa_G0000
type: SequenceAnnotation
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display id: annotation2414457
version: 1
top level: sfGFP_T65C
location: https://synbiohub.org/public/igem/BBa_K1362170/annotation2414457/range2414457/1
component: BBa_G0000

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type: Range
persistent identity: range2414457
display id: range2414457
version: 1
direction: direction/reverse
top level: sfGFP_T65C
start: 30
end: 35
orientation: inline

BBa_K1362468
type: SequenceAnnotation
persistent identity: annotation2414460
display id: annotation2414460
version: 1
top level: sfGFP_T65C
location: https://synbiohub.org/public/igem/BBa_K1362170/annotation2414460/range2414460/1
component: BBa_K1362468

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type: Range
persistent identity: range2414460
display id: range2414460
version: 1
direction: direction/reverse
top level: sfGFP_T65C
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end: 56
orientation: inline

BBa_K1758377
type: ComponentDefinition
persistent identity: BBa K1758377
display id: BBa_K1758377
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Biosensor device for detection of GHB and GBL
creator: Team Bielefeld-CeBiTec 2015
created: 2015-09-09T11:00:00Z
modified: 2015-09-18T07:41:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2180_
m user id: 26563
owner id: 26563
owning group id: 9
status: status/Planning
bookmark: true
mutable description: This part serves as a generator of sfGFP when induced.
mutable notes: ---
mutable provenance: Biobrick assembly
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1758376
component: BBa_K1758370
sequence annotation: BBa_K1758376
sequence annotation: BBa_K1758370
sequence: https://synbiohub.org/public/igem/BBa_K1758377_sequence/1

BBa_K1758376
type: Component
persistent identity: component2448545
display id: component2448545
version: 1
top level: BBa_K1758377
access: public
definition: BBa_K1758376

BBa_K1758370
type: Component
persistent identity: component2448548
display id: component2448548
version: 1
top level: BBa_K1758377
access: public
definition: https://synbiohub.org/public/igem/BBa_K1758370/1

BBa_K1758376
type: SequenceAnnotation
persistent identity: annotation2448545
display id: annotation2448545
version: 1
top level: BBa_K1758377
location: https://synbiohub.org/public/igem/BBa_K1758377/annotation2448545/range2448545/1
component: BBa_K1758376

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type: Range
persistent identity: range2448545
display id: range2448545
version: 1
direction: direction/reverse
top level: BBa_K1758377
start: 1
end: 966
orientation: inline

BBa_K1758370
type: SequenceAnnotation
persistent identity: annotation2448548
display id: annotation2448548
version: 1
top level: BBa_K1758377
location: https://synbiohub.org/public/igem/BBa_K1758377/annotation2448548/range2448548/1
component: BBa_K1758370

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type: Range
persistent identity: range2448548
display id: range2448548
version: 1
direction: direction/reverse
top level: BBa_K1758377
start: 975
end: 1863
orientation: inline

1NpuE(N*)
type: ComponentDefinition
persistent identity: BBa K1362102
display id: BBa_K1362102
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS + NpuDnaE N-intein RFC[105] non-splicing control construct (with His6)
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B??scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch??fer, Carolin Schmelas, Silvan Schmitz, Max Waldha
created: 2014-10-07T11:00:00Z
modified: 2015-05-08T01:10:04Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1738_
m user id: 0
owner id: 12377
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: ???
mutable notes: ???
mutable provenance: ???
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1362414
component: BBa_K1362421
component: BBa_K1362400
component: BBa_K1362416
component: BBa_K1362422
component: BBa_G0000
component: BBa_K1362090
component: BBa_K1362437
component: BBa_K1362417
component: BBa_K1362430
sequence annotation: BBa_K1362437
sequence annotation: BBa_K1362430
sequence annotation: BBa_K1362417
sequence annotation: BBa_K1362414
sequence annotation: BBa_K1362421
sequence annotation: BBa_K1362422
sequence annotation: BBa_K1362416
sequence annotation: BBa_K1362090
sequence annotation: BBa_K1362400
sequence annotation: BBa_G0000
sequence: https://synbiohub.org/public/igem/BBa_K1362102_sequence/1

BBa_K1362414
type: Component
persistent identity: component2410097
display id: component2410097
version: 1
top level: 1NpuE(N*)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362414/1

BBa_K1362421
type: Component
persistent identity: component2410128
display id: component2410128
version: 1
top level: 1NpuE(N*)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362421/1

BBa_K1362400
type: Component
persistent identity: component2410120
display id: component2410120
version: 1
top level: 1NpuE(N*)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362400/1

BBa_K1362416
type: Component
persistent identity: component2410119
display id: component2410119
version: 1
top level: 1NpuE(N*)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362416/1

BBa_K1362422
type: Component
persistent identity: component2410118
display id: component2410118
version: 1
top level: 1NpuE(N*)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362422/1

BBa_G0000
type: Component
persistent identity: component2410096
display id: component2410096
version: 1
top level: 1NpuE(N*)
access: public
definition: https://synbiohub.org/public/igem/BBa_G0000/1

BBa_K1362090
type: Component
persistent identity: component2410095
display id: component2410095
version: 1
top level: 1NpuE(N*)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362090/1

BBa_K1362437
type: Component
persistent identity: component2410130
display id: component2410130
version: 1
top level: 1NpuE(N*)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362437/1

BBa_K1362417
type: Component
persistent identity: component2410123
display id: component2410123
version: 1
top level: 1NpuE(N*)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362417/1

BBa_K1362430
type: Component
persistent identity: component2410126
display id: component2410126
version: 1
top level: 1NpuE(N*)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362430/1

BBa_K1362437
type: SequenceAnnotation
persistent identity: annotation2410130
display id: annotation2410130
version: 1
top level: 1NpuE(N*)
location: https://synbiohub.org/public/igem/BBa_K1362102/annotation2410130/range2410130/1
component: BBa_K1362437

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type: Range
persistent identity: range2410130
display id: range2410130
version: 1
direction: direction/reverse
top level: 1NpuE(N*)
start: 1462
end: 1464
orientation: inline

BBa_K1362430
type: SequenceAnnotation
persistent identity: annotation2410126
display id: annotation2410126
version: 1
top level: 1NpuE(N*)
location: https://synbiohub.org/public/igem/BBa_K1362102/annotation2410126/range2410126/1
component: BBa_K1362430

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type: Range
persistent identity: range2410126
display id: range2410126
version: 1
direction: direction/reverse
top level: 1NpuE(N*)
start: 1434
end: 1457
orientation: inline

BBa_K1362417
type: SequenceAnnotation
persistent identity: annotation2410123
display id: annotation2410123
version: 1
top level: 1NpuE(N*)
location: https://synbiohub.org/public/igem/BBa_K1362102/annotation2410123/range2410123/1
component: BBa_K1362417

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type: Range
persistent identity: range2410123
display id: range2410123
version: 1
direction: direction/reverse
top level: 1NpuE(N*)
start: 1428
end: 1433
orientation: inline

BBa_K1362414
type: SequenceAnnotation
persistent identity: annotation2410097
display id: annotation2410097
version: 1
top level: 1NpuE(N*)
location: https://synbiohub.org/public/igem/BBa_K1362102/annotation2410097/range2410097/1
component: BBa_K1362414

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type: Range
persistent identity: range2410097
display id: range2410097
version: 1
direction: direction/reverse
top level: 1NpuE(N*)
start: 36
end: 38
orientation: inline

BBa_K1362421
type: SequenceAnnotation
persistent identity: annotation2410128
display id: annotation2410128
version: 1
top level: 1NpuE(N*)
location: https://synbiohub.org/public/igem/BBa_K1362102/annotation2410128/range2410128/1
component: BBa_K1362421

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type: Range
persistent identity: range2410128
display id: range2410128
version: 1
direction: direction/reverse
top level: 1NpuE(N*)
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end: 1461
orientation: inline

BBa_K1362422
type: SequenceAnnotation
persistent identity: annotation2410118
display id: annotation2410118
version: 1
top level: 1NpuE(N*)
location: https://synbiohub.org/public/igem/BBa_K1362102/annotation2410118/range2410118/1
component: BBa_K1362422

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type: Range
persistent identity: range2410118
display id: range2410118
version: 1
direction: direction/reverse
top level: 1NpuE(N*)
start: 39
end: 1121
orientation: inline

BBa_K1362416
type: SequenceAnnotation
persistent identity: annotation2410119
display id: annotation2410119
version: 1
top level: 1NpuE(N*)
location: https://synbiohub.org/public/igem/BBa_K1362102/annotation2410119/range2410119/1
component: BBa_K1362416

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type: Range
persistent identity: range2410119
display id: range2410119
version: 1
direction: direction/reverse
top level: 1NpuE(N*)
start: 1122
end: 1125
orientation: inline

BBa_K1362090
type: SequenceAnnotation
persistent identity: annotation2410095
display id: annotation2410095
version: 1
top level: 1NpuE(N*)
location: https://synbiohub.org/public/igem/BBa_K1362102/annotation2410095/range2410095/1
component: BBa_K1362090

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type: Range
persistent identity: range2410095
display id: range2410095
version: 1
direction: direction/reverse
top level: 1NpuE(N*)
start: 1
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orientation: inline

BBa_K1362400
type: SequenceAnnotation
persistent identity: annotation2410120
display id: annotation2410120
version: 1
top level: 1NpuE(N*)
location: https://synbiohub.org/public/igem/BBa_K1362102/annotation2410120/range2410120/1
component: BBa_K1362400

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type: Range
persistent identity: range2410120
display id: range2410120
version: 1
direction: direction/reverse
top level: 1NpuE(N*)
start: 1126
end: 1427
orientation: inline

BBa_G0000
type: SequenceAnnotation
persistent identity: annotation2410096
display id: annotation2410096
version: 1
top level: 1NpuE(N*)
location: https://synbiohub.org/public/igem/BBa_K1362102/annotation2410096/range2410096/1
component: BBa_G0000

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type: Range
persistent identity: range2410096
display id: range2410096
version: 1
direction: direction/reverse
top level: 1NpuE(N*)
start: 30
end: 35
orientation: inline

RBS-His-Sc
type: ComponentDefinition
persistent identity: BBa K1602031
display id: BBa_K1602031
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: HisTag-Scaffold_Si4-Tag fusion protein with RBS BBa_B0034
creator: Sebastian Barthel, Niklas Hummel, Jasmin Weber, Michelle Zoeller, Marcus Geissler, Stefan Bohn, Simon Linder
created: 2015-09-15T11:00:00Z
modified: 2015-09-16T07:00:33Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2019_
m user id: 22022
owner id: 22022
owning group id: 9
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0034
component: BBa_K1602030
sequence annotation: BBa_B0034
sequence annotation: BBa_K1602030
sequence: https://synbiohub.org/public/igem/BBa_K1602031_sequence/1

BBa_B0034
type: Component
persistent identity: component2462920
display id: component2462920
version: 1
top level: RBS-His-Sc
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1602030
type: Component
persistent identity: component2462921
display id: component2462921
version: 1
top level: RBS-His-Sc
access: public
definition: https://synbiohub.org/public/igem/BBa_K1602030/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2462920
display id: annotation2462920
version: 1
top level: RBS-His-Sc
location: https://synbiohub.org/public/igem/BBa_K1602031/annotation2462920/range2462920/1
component: BBa_B0034

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type: Range
persistent identity: range2462920
display id: range2462920
version: 1
direction: direction/reverse
top level: RBS-His-Sc
start: 1
end: 12
orientation: inline

BBa_K1602030
type: SequenceAnnotation
persistent identity: annotation2462921
display id: annotation2462921
version: 1
top level: RBS-His-Sc
location: https://synbiohub.org/public/igem/BBa_K1602031/annotation2462921/range2462921/1
component: BBa_K1602030

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type: Range
persistent identity: range2462921
display id: range2462921
version: 1
direction: direction/reverse
top level: RBS-His-Sc
start: 19
end: 951
orientation: inline

BBa_K1362057
type: ComponentDefinition
persistent identity: BBa K1362057
display id: BBa_K1362057
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NLS as RFC[105] N-terminal insert
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B??scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch??fer, Carolin Schmelas, Silvan Schmitz, Max Waldha
created: 2014-10-07T11:00:00Z
modified: 2015-05-08T01:10:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1738_
m user id: 0
owner id: 22830
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000324
role: partType/Tag
component: BBa_K1362425
component: BBa_K1362440
component: BBa_J63008
component: BBa_K1362423
component: BBa_K1362444
component: BBa_K1362415
sequence annotation: BBa_K1362415
sequence annotation: BBa_K1362423
sequence annotation: BBa_J63008
sequence annotation: BBa_K1362444
sequence annotation: BBa_K1362425
sequence annotation: BBa_K1362440
sequence: https://synbiohub.org/public/igem/BBa_K1362057_sequence/1

BBa_K1362425
type: Component
persistent identity: component2409725
display id: component2409725
version: 1
top level: BBa_K1362057
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362425/1

BBa_K1362440
type: Component
persistent identity: component2409726
display id: component2409726
version: 1
top level: BBa_K1362057
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362440/1

BBa_J63008
type: Component
persistent identity: component2409727
display id: component2409727
version: 1
top level: BBa_K1362057
access: public
definition: https://synbiohub.org/public/igem/BBa_J63008/1

BBa_K1362423
type: Component
persistent identity: component2409730
display id: component2409730
version: 1
top level: BBa_K1362057
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362423/1

BBa_K1362444
type: Component
persistent identity: component2409728
display id: component2409728
version: 1
top level: BBa_K1362057
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362444/1

BBa_K1362415
type: Component
persistent identity: component2409729
display id: component2409729
version: 1
top level: BBa_K1362057
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362415/1

BBa_K1362415
type: SequenceAnnotation
persistent identity: annotation2409729
display id: annotation2409729
version: 1
top level: BBa_K1362057
location: https://synbiohub.org/public/igem/BBa_K1362057/annotation2409729/range2409729/1
component: BBa_K1362415

https://synbiohub.org/public/igem/BBa_K1362057/annotation2409729/range2409729/1
type: Range
persistent identity: range2409729
display id: range2409729
version: 1
direction: direction/reverse
top level: BBa_K1362057
start: 50
end: 53
orientation: inline

BBa_K1362423
type: SequenceAnnotation
persistent identity: annotation2409730
display id: annotation2409730
version: 1
top level: BBa_K1362057
location: https://synbiohub.org/public/igem/BBa_K1362057/annotation2409730/range2409730/1
component: BBa_K1362423

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type: Range
persistent identity: range2409730
display id: range2409730
version: 1
direction: direction/reverse
top level: BBa_K1362057
start: 54
end: 60
orientation: inline

BBa_J63008
type: SequenceAnnotation
persistent identity: annotation2409727
display id: annotation2409727
version: 1
top level: BBa_K1362057
location: https://synbiohub.org/public/igem/BBa_K1362057/annotation2409727/range2409727/1
component: BBa_J63008

https://synbiohub.org/public/igem/BBa_K1362057/annotation2409727/range2409727/1
type: Range
persistent identity: range2409727
display id: range2409727
version: 1
direction: direction/reverse
top level: BBa_K1362057
start: 11
end: 31
orientation: inline

BBa_K1362444
type: SequenceAnnotation
persistent identity: annotation2409728
display id: annotation2409728
version: 1
top level: BBa_K1362057
location: https://synbiohub.org/public/igem/BBa_K1362057/annotation2409728/range2409728/1
component: BBa_K1362444

https://synbiohub.org/public/igem/BBa_K1362057/annotation2409728/range2409728/1
type: Range
persistent identity: range2409728
display id: range2409728
version: 1
direction: direction/reverse
top level: BBa_K1362057
start: 32
end: 49
orientation: inline

BBa_K1362425
type: SequenceAnnotation
persistent identity: annotation2409725
display id: annotation2409725
version: 1
top level: BBa_K1362057
location: https://synbiohub.org/public/igem/BBa_K1362057/annotation2409725/range2409725/1
component: BBa_K1362425

https://synbiohub.org/public/igem/BBa_K1362057/annotation2409725/range2409725/1
type: Range
persistent identity: range2409725
display id: range2409725
version: 1
direction: direction/reverse
top level: BBa_K1362057
start: 1
end: 6
orientation: inline

BBa_K1362440
type: SequenceAnnotation
persistent identity: annotation2409726
display id: annotation2409726
version: 1
top level: BBa_K1362057
location: https://synbiohub.org/public/igem/BBa_K1362057/annotation2409726/range2409726/1
component: BBa_K1362440

https://synbiohub.org/public/igem/BBa_K1362057/annotation2409726/range2409726/1
type: Range
persistent identity: range2409726
display id: range2409726
version: 1
direction: direction/reverse
top level: BBa_K1362057
start: 7
end: 10
orientation: inline

BBa_K1321163
type: ComponentDefinition
persistent identity: BBa K1321163
display id: BBa_K1321163
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NiBP driven by T7 promoter
creator: Gabriella Santosa
created: 2014-10-08T11:00:00Z
modified: 2015-05-08T01:09:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 0
owner id: 20961
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: GATC
mutable notes: GATC
mutable provenance: GATC
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1321338
component: BBa_K1321009
component: BBa_K1319106
sequence annotation: BBa_K1319106
sequence annotation: BBa_K1321338
sequence annotation: BBa_K1321009
sequence: https://synbiohub.org/public/igem/BBa_K1321163_sequence/1

BBa_K1321338
type: Component
persistent identity: component2415074
display id: component2415074
version: 1
top level: BBa_K1321163
access: public
definition: BBa_K1321338

BBa_K1321009
type: Component
persistent identity: component2415077
display id: component2415077
version: 1
top level: BBa_K1321163
access: public
definition: BBa_K1321009

BBa_K1319106
type: Component
persistent identity: component2415076
display id: component2415076
version: 1
top level: BBa_K1321163
access: public
definition: https://synbiohub.org/public/igem/BBa_K1319106/1

BBa_K1319106
type: SequenceAnnotation
persistent identity: annotation2415076
display id: annotation2415076
version: 1
top level: BBa_K1321163
location: https://synbiohub.org/public/igem/BBa_K1321163/annotation2415076/range2415076/1
component: BBa_K1319106

https://synbiohub.org/public/igem/BBa_K1321163/annotation2415076/range2415076/1
type: Range
persistent identity: range2415076
display id: range2415076
version: 1
direction: direction/reverse
top level: BBa_K1321163
start: 44
end: 58
orientation: inline

BBa_K1321338
type: SequenceAnnotation
persistent identity: annotation2415074
display id: annotation2415074
version: 1
top level: BBa_K1321163
location: https://synbiohub.org/public/igem/BBa_K1321163/annotation2415074/range2415074/1
component: BBa_K1321338

https://synbiohub.org/public/igem/BBa_K1321163/annotation2415074/range2415074/1
type: Range
persistent identity: range2415074
display id: range2415074
version: 1
direction: direction/reverse
top level: BBa_K1321163
start: 1
end: 43
orientation: inline

BBa_K1321009
type: SequenceAnnotation
persistent identity: annotation2415077
display id: annotation2415077
version: 1
top level: BBa_K1321163
location: https://synbiohub.org/public/igem/BBa_K1321163/annotation2415077/range2415077/1
component: BBa_K1321009

https://synbiohub.org/public/igem/BBa_K1321163/annotation2415077/range2415077/1
type: Range
persistent identity: range2415077
display id: range2415077
version: 1
direction: direction/reverse
top level: BBa_K1321163
start: 59
end: 234
orientation: inline

T7 express
type: ComponentDefinition
persistent identity: BBa K1639012
display id: BBa_K1639012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 expression system for pSB1C3
creator: Nurgeldi Bazarov
created: 2015-09-14T11:00:00Z
modified: 2015-09-18T06:55:31Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2056_
m user id: 20835
owner id: 24789
owning group id: 9
status: status/Planning
bookmark: true
mutable description: In our project we needed to express two different genes at the same bacteria, but unfortunately because of high repeats of sequences gene synthesizing company couldn't synthesize it. To solve this we just divided our genes into different plasmids and cotransformed it into same bacteria. For coexpression of genes from different plasmids origins have to be compatible, like ColA and ColE1. pSB1C3 possess ColE1 origin of replication. So it's compatible for expression with plasmid containg ColA origin of replication. But it doesn't contain expression machinery like promoters, terminators. We inserted this part to modify pSB1C3 so that it can be used as expression vector.
mutable notes: We inserted BamH1 and Sal1 cut sites between T7 promoter and T7 terminator. This part also contain RBS. Any other team can use this part to modify vectors like pSB1C3 to add T7 expression feature. Just clone it with enzymes at prefix and suffix.
mutable provenance: It is combination of T7 promoter-terminator with sequences containing restriction enzyme cut sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: T7 terminator
sequence annotation: T7 bacteriophage RBS
sequence annotation: T7 promoter
sequence: https://synbiohub.org/public/igem/BBa_K1639012_sequence/1

T7 terminator
type: SequenceAnnotation
persistent identity: annotation2457604
display id: annotation2457604
version: 1
top level: T7 express
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1639012/annotation2457604/range2457604/1

https://synbiohub.org/public/igem/BBa_K1639012/annotation2457604/range2457604/1
type: Range
persistent identity: range2457604
display id: range2457604
version: 1
direction: direction/forward
top level: T7 express
start: 124
end: 171
orientation: inline

T7 bacteriophage RBS
type: SequenceAnnotation
persistent identity: annotation2457603
display id: annotation2457603
version: 1
top level: T7 express
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1639012/annotation2457603/range2457603/1

https://synbiohub.org/public/igem/BBa_K1639012/annotation2457603/range2457603/1
type: Range
persistent identity: range2457603
display id: range2457603
version: 1
direction: direction/forward
top level: T7 express
start: 35
end: 57
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2457602
display id: annotation2457602
version: 1
top level: T7 express
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1639012/annotation2457602/range2457602/1

https://synbiohub.org/public/igem/BBa_K1639012/annotation2457602/range2457602/1
type: Range
persistent identity: range2457602
display id: range2457602
version: 1
direction: direction/forward
top level: T7 express
start: 1
end: 19
orientation: inline

BBa_K1365169
type: ComponentDefinition
persistent identity: BBa K1365169
display id: BBa_K1365169
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: signal sequence from USP45 fused to aiiA
creator: Rick Elbert
created: 2014-09-29T11:00:00Z
modified: 2015-05-08T01:10:07Z
discontinued: false
dominant: false
group u list: _1741_
m user id: 0
owner id: 20186
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: ----------------
mutable notes: - one nucleotide in the signal sequence of USP45 had to be changed because it contained the illegal restriction site PstI
mutable provenance: -aiiA was isolated out of the biobrick BBa_C0060. -ssUSP45 was isolated from the synthetic gene ssUSP45DspB made by IDT.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: aiiA
sequence annotation: ssUSP45
sequence annotation: start
sequence annotation: TAATAA
sequence: https://synbiohub.org/public/igem/BBa_K1365169_sequence/1

aiiA
type: SequenceAnnotation
persistent identity: annotation2390761
display id: annotation2390761
version: 1
top level: BBa_K1365169
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1365169/annotation2390761/range2390761/1

https://synbiohub.org/public/igem/BBa_K1365169/annotation2390761/range2390761/1
type: Range
persistent identity: range2390761
display id: range2390761
version: 1
direction: direction/forward
top level: BBa_K1365169
start: 81
end: 827
orientation: inline

ssUSP45
type: SequenceAnnotation
persistent identity: annotation2390760
display id: annotation2390760
version: 1
top level: BBa_K1365169
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1365169/annotation2390760/range2390760/1

https://synbiohub.org/public/igem/BBa_K1365169/annotation2390760/range2390760/1
type: Range
persistent identity: range2390760
display id: range2390760
version: 1
direction: direction/forward
top level: BBa_K1365169
start: 1
end: 80
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2423467
display id: annotation2423467
version: 1
top level: BBa_K1365169
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1365169/annotation2423467/range2423467/1

https://synbiohub.org/public/igem/BBa_K1365169/annotation2423467/range2423467/1
type: Range
persistent identity: range2423467
display id: range2423467
version: 1
direction: direction/forward
top level: BBa_K1365169
start: 1
end: 1
orientation: inline

TAATAA
type: SequenceAnnotation
persistent identity: annotation2390762
display id: annotation2390762
version: 1
top level: BBa_K1365169
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1365169/annotation2390762/range2390762/1

https://synbiohub.org/public/igem/BBa_K1365169/annotation2390762/range2390762/1
type: Range
persistent identity: range2390762
display id: range2390762
version: 1
direction: direction/forward
top level: BBa_K1365169
start: 828
end: 833
orientation: inline

BBa_K1472601
type: ComponentDefinition
persistent identity: BBa K1472601
display id: BBa_K1472601
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Leaderless thioesterase
creator: Lo Hiu Fung, Lau Tsz Kit, Chu Lok Ting, Wu Chun Ting
created: 2014-09-15T11:00:00Z
modified: 2015-05-08T01:10:39Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1851_
m user id: 0
owner id: 22977
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: We have cloned a modified thioesterase gene, ???tesA, which codes for an E. coli thioesterase protein that lacks the N-terminal 26-amino acid signal peptide sequence, and is named here as ???leaderless TesA??? or ???TesA. The ???TesA protein is expected to be expressed exclusively in the cytosol of E. coli to catalyze the conversion of fatty acyl-ACP (acyl carrier protein) or fatty acyl-CoA to free fatty acids (Figure 1). ???TesA is predicted to enhance free fatty acid production in the genetically-modified E. coli strain.
mutable notes: N/A
mutable provenance: The genome sequence of Escherichia coli K-12 substr. W3110 is available in GenBank at http://www.ncbi.nlm.nih.gov/gene/12930860.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: 'tesA
sequence: https://synbiohub.org/public/igem/BBa_K1472601_sequence/1

'tesA
type: SequenceAnnotation
persistent identity: annotation2383718
display id: annotation2383718
version: 1
top level: BBa_K1472601
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1472601/annotation2383718/range2383718/1

https://synbiohub.org/public/igem/BBa_K1472601/annotation2383718/range2383718/1
type: Range
persistent identity: range2383718
display id: range2383718
version: 1
direction: direction/forward
top level: BBa_K1472601
start: 1
end: 549
orientation: inline

BBa_K1789004
type: ComponentDefinition
persistent identity: BBa K1789004
display id: BBa_K1789004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP2
creator: Xinyuan Qiu
created: 2015-09-13T11:00:00Z
modified: 2016-01-25T01:04:48Z
discontinued: false
dominant: false
group u list: _2214_
m user id: 4206
owner id: 21134
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This part is the Carboxyl Half of GFP
mutable notes: we REMOVED the extra base from part BBa_I715020(easier to fuse with other proteins) when designing PCR primers to make the protein fusion easier.
mutable provenance: This part is gained from part BBa_I715020.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: GFP2
sequence: https://synbiohub.org/public/igem/BBa_K1789004_sequence/1

GFP2
type: SequenceAnnotation
persistent identity: annotation2454067
display id: annotation2454067
version: 1
top level: BBa_K1789004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1789004/annotation2454067/range2454067/1

https://synbiohub.org/public/igem/BBa_K1789004/annotation2454067/range2454067/1
type: Range
persistent identity: range2454067
display id: range2454067
version: 1
direction: direction/forward
top level: BBa_K1789004
start: 1
end: 249
orientation: inline

BBa_K1598001
type: ComponentDefinition
persistent identity: BBa K1598001
display id: BBa_K1598001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TPH1 (Tryptophan hydroxylase 1)
creator: Marta Napiorkowska
created: 2015-06-17T11:00:00Z
modified: 2015-08-27T02:25:18Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2015_
m user id: 27180
owner id: 27256
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Converts the amino acid tryptophan into 5-HTP, which is a precursor to serotonin. Soon to be edit
mutable notes: Optimised for E.coli, removed illegal restriction enzyme sites
mutable provenance: Human (Homo sapiens)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1598001_sequence/1

BBa_K1472610
type: ComponentDefinition
persistent identity: BBa K1472610
display id: BBa_K1472610
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Delta 15 desaturase
creator: Choi hiu Ying, LEE Cheuk Lam, Tse Yui Shing, Yeung Sin Yu
created: 2014-09-27T11:00:00Z
modified: 2015-05-08T01:10:39Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1851_
m user id: 0
owner id: 23007
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This sequence encoded for the delta15 desaturase. The gene is from Synechocystis sp. and optimised for the expression in E. coli. During the fatty acid conversion process, delta 15 desaturase catalyses the conversion of linolenic acid (18:2) into alpha-Linolenic acid, ALA (18:3) by adding a cis-doube bond at carbon 15.
mutable notes: The enzyme cut site must be designed according to iGEM prefix and suffix requirements.
mutable provenance: The amino acid sequence of delta 15 desaturase is derived from Synechocystis sp. but codon optimised for the expression in E. coli.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Delta 15 desaturase
sequence: https://synbiohub.org/public/igem/BBa_K1472610_sequence/1

Delta 15 desaturase
type: SequenceAnnotation
persistent identity: annotation2390390
display id: annotation2390390
version: 1
top level: BBa_K1472610
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1472610/annotation2390390/range2390390/1

https://synbiohub.org/public/igem/BBa_K1472610/annotation2390390/range2390390/1
type: Range
persistent identity: range2390390
display id: range2390390
version: 1
direction: direction/forward
top level: BBa_K1472610
start: 1
end: 1104
orientation: inline

Mcfp3
type: ComponentDefinition
persistent identity: BBa K1592001
display id: BBa_K1592001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Mytilus californianus foot protein 3(Mcfp3) variant 3
creator: Shuyan Tang
created: 2015-08-29T11:00:00Z
modified: 2015-09-18T09:23:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2009_
m user id: 20267
owner id: 20267
owning group id: 9
status: status/Planning
bookmark: true
mutable description: This is the cell display system of Yarrowia lipolytica, composed of LIP prepro, interest protein, and YLcwp3. LIP prepro is signal peptide used to secrete the interest protein out of the cell, and the YLcwp3 is the anchor domain binding the interest protein to the cell wall of yeast. We use this system to display silica-tag and test its binding characteristics. E.coli ribosomal protein L2 was found to bind tightly to silicon particles, which have surfaces that are oxidized to silica. This L2 silica-binding tag, called the ??????Si-tag,?????? can be used for one-step targeting of functional proteins on silica surfaces. The silica-binding domains of E. coli L2 was mapped to amino acids 1???60, 61-202 and 203???273. We respectively test the silica-binding characteristics of this three regions and their combinations. E. coli ribosomal protein L2 (1-60) is the amino acids 1???60 of E. coli ribosomal protein L2, we call it si-tag1.
mutable notes: No.
mutable provenance: We got the LIP prepro and YLcwp3 sequence from our lab, and synthetize the E. coli ribosomal protein L2 (1-60) by IDT.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Start codon
sequence: https://synbiohub.org/public/igem/BBa_K1592001_sequence/1

Start codon
type: SequenceAnnotation
persistent identity: annotation2455905
display id: annotation2455905
version: 1
top level: Mcfp3
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1592001/annotation2455905/range2455905/1

https://synbiohub.org/public/igem/BBa_K1592001/annotation2455905/range2455905/1
type: Range
persistent identity: range2455905
display id: range2455905
version: 1
direction: direction/forward
top level: Mcfp3
start: 1
end: 3
orientation: inline

BBa_K1362055
type: ComponentDefinition
persistent identity: BBa K1362055
display id: BBa_K1362055
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: MinD MTS as RFC[105] C-terminal insert
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B??scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch??fer, Carolin Schmelas, Silvan Schmitz, Max Waldha
created: 2014-10-07T11:00:00Z
modified: 2015-05-08T01:10:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1738_
m user id: 0
owner id: 22830
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1362426
component: BBa_K1362419
component: BBa_K1362421
component: BBa_K1362433
component: BBa_K1362423
component: BBa_K1362438
component: BBa_K1362425
component: BBa_K1362255
sequence annotation: BBa_K1362438
sequence annotation: BBa_K1362426
sequence annotation: BBa_K1362419
sequence annotation: BBa_K1362421
sequence annotation: BBa_K1362423
sequence annotation: BBa_K1362255
sequence annotation: BBa_K1362425
sequence annotation: BBa_K1362433
sequence: https://synbiohub.org/public/igem/BBa_K1362055_sequence/1

BBa_K1362426
type: Component
persistent identity: component2409715
display id: component2409715
version: 1
top level: BBa_K1362055
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362426/1

BBa_K1362419
type: Component
persistent identity: component2409707
display id: component2409707
version: 1
top level: BBa_K1362055
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362419/1

BBa_K1362421
type: Component
persistent identity: component2409713
display id: component2409713
version: 1
top level: BBa_K1362055
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362421/1

BBa_K1362433
type: Component
persistent identity: component2409709
display id: component2409709
version: 1
top level: BBa_K1362055
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362433/1

BBa_K1362423
type: Component
persistent identity: component2409714
display id: component2409714
version: 1
top level: BBa_K1362055
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362423/1

BBa_K1362438
type: Component
persistent identity: component2409710
display id: component2409710
version: 1
top level: BBa_K1362055
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362438/1

BBa_K1362425
type: Component
persistent identity: component2409706
display id: component2409706
version: 1
top level: BBa_K1362055
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362425/1

BBa_K1362255
type: Component
persistent identity: component2409711
display id: component2409711
version: 1
top level: BBa_K1362055
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362255/1

BBa_K1362438
type: SequenceAnnotation
persistent identity: annotation2409710
display id: annotation2409710
version: 1
top level: BBa_K1362055
location: https://synbiohub.org/public/igem/BBa_K1362055/annotation2409710/range2409710/1
component: BBa_K1362438

https://synbiohub.org/public/igem/BBa_K1362055/annotation2409710/range2409710/1
type: Range
persistent identity: range2409710
display id: range2409710
version: 1
direction: direction/reverse
top level: BBa_K1362055
start: 20
end: 28
orientation: inline

BBa_K1362426
type: SequenceAnnotation
persistent identity: annotation2409715
display id: annotation2409715
version: 1
top level: BBa_K1362055
location: https://synbiohub.org/public/igem/BBa_K1362055/annotation2409715/range2409715/1
component: BBa_K1362426

https://synbiohub.org/public/igem/BBa_K1362055/annotation2409715/range2409715/1
type: Range
persistent identity: range2409715
display id: range2409715
version: 1
direction: direction/reverse
top level: BBa_K1362055
start: 112
end: 115
orientation: inline

BBa_K1362419
type: SequenceAnnotation
persistent identity: annotation2409707
display id: annotation2409707
version: 1
top level: BBa_K1362055
location: https://synbiohub.org/public/igem/BBa_K1362055/annotation2409707/range2409707/1
component: BBa_K1362419

https://synbiohub.org/public/igem/BBa_K1362055/annotation2409707/range2409707/1
type: Range
persistent identity: range2409707
display id: range2409707
version: 1
direction: direction/reverse
top level: BBa_K1362055
start: 7
end: 10
orientation: inline

BBa_K1362421
type: SequenceAnnotation
persistent identity: annotation2409713
display id: annotation2409713
version: 1
top level: BBa_K1362055
location: https://synbiohub.org/public/igem/BBa_K1362055/annotation2409713/range2409713/1
component: BBa_K1362421

https://synbiohub.org/public/igem/BBa_K1362055/annotation2409713/range2409713/1
type: Range
persistent identity: range2409713
display id: range2409713
version: 1
direction: direction/reverse
top level: BBa_K1362055
start: 101
end: 104
orientation: inline

BBa_K1362423
type: SequenceAnnotation
persistent identity: annotation2409714
display id: annotation2409714
version: 1
top level: BBa_K1362055
location: https://synbiohub.org/public/igem/BBa_K1362055/annotation2409714/range2409714/1
component: BBa_K1362423

https://synbiohub.org/public/igem/BBa_K1362055/annotation2409714/range2409714/1
type: Range
persistent identity: range2409714
display id: range2409714
version: 1
direction: direction/reverse
top level: BBa_K1362055
start: 105
end: 111
orientation: inline

BBa_K1362255
type: SequenceAnnotation
persistent identity: annotation2409711
display id: annotation2409711
version: 1
top level: BBa_K1362055
location: https://synbiohub.org/public/igem/BBa_K1362055/annotation2409711/range2409711/1
component: BBa_K1362255

https://synbiohub.org/public/igem/BBa_K1362055/annotation2409711/range2409711/1
type: Range
persistent identity: range2409711
display id: range2409711
version: 1
direction: direction/reverse
top level: BBa_K1362055
start: 29
end: 100
orientation: inline

BBa_K1362425
type: SequenceAnnotation
persistent identity: annotation2409706
display id: annotation2409706
version: 1
top level: BBa_K1362055
location: https://synbiohub.org/public/igem/BBa_K1362055/annotation2409706/range2409706/1
component: BBa_K1362425

https://synbiohub.org/public/igem/BBa_K1362055/annotation2409706/range2409706/1
type: Range
persistent identity: range2409706
display id: range2409706
version: 1
direction: direction/reverse
top level: BBa_K1362055
start: 1
end: 6
orientation: inline

BBa_K1362433
type: SequenceAnnotation
persistent identity: annotation2409709
display id: annotation2409709
version: 1
top level: BBa_K1362055
location: https://synbiohub.org/public/igem/BBa_K1362055/annotation2409709/range2409709/1
component: BBa_K1362433

https://synbiohub.org/public/igem/BBa_K1362055/annotation2409709/range2409709/1
type: Range
persistent identity: range2409709
display id: range2409709
version: 1
direction: direction/reverse
top level: BBa_K1362055
start: 11
end: 19
orientation: inline

BBa_K1758333
type: ComponentDefinition
persistent identity: BBa K1758333
display id: BBa_K1758333
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lead repressor under control of constitutive promoter and strong RBS and lead responsive promoter wi
creator: Team Bielefeld-CeBiTec 2015
created: 2015-08-29T11:00:00Z
modified: 2016-01-06T03:41:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2180_
m user id: 4206
owner id: 26630
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Repressor for lead responsive promoter under the control of konstitutive promoter and lead responsive promoter with 5??untranslated region and sfGFP for detection. As a lead biosensor
mutable notes: ??...
mutable provenance: genomic sequence out of ????Cupriavidus metallidurans???? CH34
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1758330
component: BBa_K1758332
sequence annotation: BBa_K1758332
sequence annotation: BBa_K1758330
sequence: https://synbiohub.org/public/igem/BBa_K1758333_sequence/1

BBa_K1758330
type: Component
persistent identity: component2440122
display id: component2440122
version: 1
top level: BBa_K1758333
access: public
definition: BBa_K1758330

BBa_K1758332
type: Component
persistent identity: component2440128
display id: component2440128
version: 1
top level: BBa_K1758333
access: public
definition: https://synbiohub.org/public/igem/BBa_K1758332/1

BBa_K1758332
type: SequenceAnnotation
persistent identity: annotation2440128
display id: annotation2440128
version: 1
top level: BBa_K1758333
location: https://synbiohub.org/public/igem/BBa_K1758333/annotation2440128/range2440128/1
component: BBa_K1758332

https://synbiohub.org/public/igem/BBa_K1758333/annotation2440128/range2440128/1
type: Range
persistent identity: range2440128
display id: range2440128
version: 1
direction: direction/reverse
top level: BBa_K1758333
start: 508
end: 1484
orientation: inline

BBa_K1758330
type: SequenceAnnotation
persistent identity: annotation2440122
display id: annotation2440122
version: 1
top level: BBa_K1758333
location: https://synbiohub.org/public/igem/BBa_K1758333/annotation2440122/range2440122/1
component: BBa_K1758330

https://synbiohub.org/public/igem/BBa_K1758333/annotation2440122/range2440122/1
type: Range
persistent identity: range2440122
display id: range2440122
version: 1
direction: direction/reverse
top level: BBa_K1758333
start: 1
end: 499
orientation: inline

BBa_K1460004
type: ComponentDefinition
persistent identity: BBa K1460004
display id: BBa_K1460004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Anderson Promoter + MerT + MerP + ter
creator: Eric Holmes
created: 2014-08-25T11:00:00Z
modified: 2016-01-13T10:25:46Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1839_
m user id: 4206
owner id: 15649
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is a functional coding sequence for the mercury transport proteins merT/merP from P. aeruginosa. This part will result in mercury intolerance due to greater mercury transport into engineered cells.
mutable notes: The Anderson promoter provides strong constitutive expression of merT/merP in E. coli
mutable provenance: Anderson promoter is that of part BBa_J23102. Terminator is that of part BBa_B1006. merT/merP genes are naturally found in Pseudomonas aeruginosa. This part was synthesized by GenScript in the vector pUC57. The part was then transferred into pSB1C3 using standard cloning procedures.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: merP
sequence annotation: BBa_J23102
sequence annotation: BBa_B1006
sequence annotation: merT
sequence annotation: BBa_J61101
sequence: https://synbiohub.org/public/igem/BBa_K1460004_sequence/1

merP
type: SequenceAnnotation
persistent identity: annotation2381486
display id: annotation2381486
version: 1
top level: BBa_K1460004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1460004/annotation2381486/range2381486/1

https://synbiohub.org/public/igem/BBa_K1460004/annotation2381486/range2381486/1
type: Range
persistent identity: range2381486
display id: range2381486
version: 1
direction: direction/forward
top level: BBa_K1460004
start: 426
end: 701
orientation: inline

BBa_J23102
type: SequenceAnnotation
persistent identity: annotation2381484
display id: annotation2381484
version: 1
top level: BBa_K1460004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1460004/annotation2381484/range2381484/1

https://synbiohub.org/public/igem/BBa_K1460004/annotation2381484/range2381484/1
type: Range
persistent identity: range2381484
display id: range2381484
version: 1
direction: direction/forward
top level: BBa_K1460004
start: 1
end: 35
orientation: inline

BBa_B1006
type: SequenceAnnotation
persistent identity: annotation2381487
display id: annotation2381487
version: 1
top level: BBa_K1460004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1460004/annotation2381487/range2381487/1

https://synbiohub.org/public/igem/BBa_K1460004/annotation2381487/range2381487/1
type: Range
persistent identity: range2381487
display id: range2381487
version: 1
direction: direction/forward
top level: BBa_K1460004
start: 702
end: 740
orientation: inline

merT
type: SequenceAnnotation
persistent identity: annotation2381485
display id: annotation2381485
version: 1
top level: BBa_K1460004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1460004/annotation2381485/range2381485/1

https://synbiohub.org/public/igem/BBa_K1460004/annotation2381485/range2381485/1
type: Range
persistent identity: range2381485
display id: range2381485
version: 1
direction: direction/forward
top level: BBa_K1460004
start: 63
end: 413
orientation: inline

BBa_J61101
type: SequenceAnnotation
persistent identity: annotation2381483
display id: annotation2381483
version: 1
top level: BBa_K1460004
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1460004/annotation2381483/range2381483/1

https://synbiohub.org/public/igem/BBa_K1460004/annotation2381483/range2381483/1
type: Range
persistent identity: range2381483
display id: range2381483
version: 1
direction: direction/forward
top level: BBa_K1460004
start: 43
end: 56
orientation: inline

BBa_K1478000
type: ComponentDefinition
persistent identity: BBa K1478000
display id: BBa_K1478000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Expa4 plant secretion signal, localizes to extracellular space
creator: Fabian Fr??mling
created: 2014-09-12T11:00:00Z
modified: 2015-05-27T02:12:26Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1858_
m user id: 4206
owner id: 20973
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Coding region for first 20 aminoacids of plant protein Exansin4. Secretion signal for plants (derived from A. thaliana). When fused to a protein, directs the protein to extracellular space.
mutable notes: Sequence was optimized for Escherichia coli and Arabidopsis thaliana codon usage.
mutable provenance: Derived from Expansin4 from Arabidopsis thaliana(NP_181500).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K1478000_sequence/1

BBa_K1321341
type: ComponentDefinition
persistent identity: BBa K1321341
display id: BBa_K1321341
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: sfGFP fused to CBDclos in RFC 25
creator: Chris N Micklem
created: 2014-10-07T11:00:00Z
modified: 2015-06-17T12:27:02Z
discontinued: false
dominant: false
group u list: _1696_
m user id: 4206
owner id: 20830
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: asdfads
mutable notes: asdfd
mutable provenance: asdfad
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_B0105
component: BBa_K1321002
component: BBa_K1321337
sequence annotation: BBa_K1321002
sequence annotation: BBa_B0105
sequence annotation: BBa_K1321337
sequence: https://synbiohub.org/public/igem/BBa_K1321341_sequence/1

BBa_B0105
type: Component
persistent identity: component2415090
display id: component2415090
version: 1
top level: BBa_K1321341
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_K1321002
type: Component
persistent identity: component2415091
display id: component2415091
version: 1
top level: BBa_K1321341
access: public
definition: https://synbiohub.org/public/igem/BBa_K1321002/1

BBa_K1321337
type: Component
persistent identity: component2415088
display id: component2415088
version: 1
top level: BBa_K1321341
access: public
definition: BBa_K1321337

BBa_K1321002
type: SequenceAnnotation
persistent identity: annotation2415091
display id: annotation2415091
version: 1
top level: BBa_K1321341
location: https://synbiohub.org/public/igem/BBa_K1321341/annotation2415091/range2415091/1
component: BBa_K1321002

https://synbiohub.org/public/igem/BBa_K1321341/annotation2415091/range2415091/1
type: Range
persistent identity: range2415091
display id: range2415091
version: 1
direction: direction/reverse
top level: BBa_K1321341
start: 718
end: 1017
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2415090
display id: annotation2415090
version: 1
top level: BBa_K1321341
location: https://synbiohub.org/public/igem/BBa_K1321341/annotation2415090/range2415090/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_K1321341/annotation2415090/range2415090/1
type: Range
persistent identity: range2415090
display id: range2415090
version: 1
direction: direction/reverse
top level: BBa_K1321341
start: 712
end: 717
orientation: inline

BBa_K1321337
type: SequenceAnnotation
persistent identity: annotation2415088
display id: annotation2415088
version: 1
top level: BBa_K1321341
location: https://synbiohub.org/public/igem/BBa_K1321341/annotation2415088/range2415088/1
component: BBa_K1321337

https://synbiohub.org/public/igem/BBa_K1321341/annotation2415088/range2415088/1
type: Range
persistent identity: range2415088
display id: range2415088
version: 1
direction: direction/reverse
top level: BBa_K1321341
start: 1
end: 711
orientation: inline

BBa_K1491015
type: ComponentDefinition
persistent identity: BBa K1491015
display id: BBa_K1491015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Codon Optimized KillerRed
creator: Danielle Peters
created: 2014-08-03T11:00:00Z
modified: 2015-05-08T01:10:44Z
discontinued: false
dominant: false
group u list: _1871_
m user id: 0
owner id: 22521
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part is the codon optimized version of KillerRed (BBa_K1184000). It has been optimized for E. coli by eliminating the rare codons.
mutable notes: Increase the functionality of KillerRed through codon optimization.
mutable provenance: This part was codon optimized from the original KillerRed (BBa_K1184000)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: 6x His Tag
sequence: https://synbiohub.org/public/igem/BBa_K1491015_sequence/1

6x His Tag
type: SequenceAnnotation
persistent identity: annotation2380344
display id: annotation2380344
version: 1
top level: BBa_K1491015
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1491015/annotation2380344/range2380344/1

https://synbiohub.org/public/igem/BBa_K1491015/annotation2380344/range2380344/1
type: Range
persistent identity: range2380344
display id: range2380344
version: 1
direction: direction/forward
top level: BBa_K1491015
start: 718
end: 735
orientation: inline

BBa_K1315011
type: ComponentDefinition
persistent identity: BBa K1315011
display id: BBa_K1315011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pseudomonas AutoInducer-1 response unit (lasB promoter)
creator: Dimitrios Michailidis
created: 2014-09-26T11:00:00Z
modified: 2015-05-08T01:09:50Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1690_
m user id: 0
owner id: 21812
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: ptet+lasR+plasB
mutable notes: All the inserts were digested with XbaI and PstI, and all the vectors were each time cut with SpeI and PstI.
mutable provenance: All the parts have come from existing iGEM distribution kits.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_R0079
component: BBa_R0040
component: BBa_C0179
sequence annotation: BBa_R0040
sequence annotation: BBa_R0079
sequence annotation: BBa_C0179
sequence: https://synbiohub.org/public/igem/BBa_K1315011_sequence/1

BBa_R0079
type: Component
persistent identity: component2387455
display id: component2387455
version: 1
top level: BBa_K1315011
access: public
definition: https://synbiohub.org/public/igem/BBa_R0079/1

BBa_R0040
type: Component
persistent identity: component2387445
display id: component2387445
version: 1
top level: BBa_K1315011
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_C0179
type: Component
persistent identity: component2387450
display id: component2387450
version: 1
top level: BBa_K1315011
access: public
definition: https://synbiohub.org/public/igem/BBa_C0179/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2387445
display id: annotation2387445
version: 1
top level: BBa_K1315011
location: https://synbiohub.org/public/igem/BBa_K1315011/annotation2387445/range2387445/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K1315011/annotation2387445/range2387445/1
type: Range
persistent identity: range2387445
display id: range2387445
version: 1
direction: direction/forward
top level: BBa_K1315011
start: 1
end: 54
orientation: inline

BBa_R0079
type: SequenceAnnotation
persistent identity: annotation2387455
display id: annotation2387455
version: 1
top level: BBa_K1315011
location: https://synbiohub.org/public/igem/BBa_K1315011/annotation2387455/range2387455/1
component: BBa_R0079

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type: Range
persistent identity: range2387455
display id: range2387455
version: 1
direction: direction/reverse
top level: BBa_K1315011
start: 792
end: 948
orientation: inline

BBa_C0179
type: SequenceAnnotation
persistent identity: annotation2387450
display id: annotation2387450
version: 1
top level: BBa_K1315011
location: https://synbiohub.org/public/igem/BBa_K1315011/annotation2387450/range2387450/1
component: BBa_C0179

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type: Range
persistent identity: range2387450
display id: range2387450
version: 1
direction: direction/reverse
top level: BBa_K1315011
start: 61
end: 783
orientation: inline

ONBY RS
type: ComponentDefinition
persistent identity: BBa K1416000
display id: BBa_K1416000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: The tRNA synthetase/tRNA needed for incorporating o-(2-nitrobenzyl)-L-tyrosine (ONBY) at a UAG codon
creator: Jordan Monk
created: 2014-10-05T11:00:00Z
modified: 2015-05-08T01:10:20Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1794_
m user id: 0
owner id: 17383
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: The part includes the gene coding for the Methanocaldococcus jannaschii synthetase mutated to charge the orthogonal tRNA (also in the part) with the non-canonical amino acid o-nitrobenzyl tyrosine (ONBY) along with the proper promoters and terminator . This part encodes for the incorporation of ONBY during translation at AUG amber stop codons in a cell with Release Factor 1 (RF-1) knocked out such as "Amberless" E. coli or "RF-0".
mutable provenance: Evolved from the Methanocaldococcus janaschii tyrosyl synthetase and tRNA by the Schulz group. Specific mutations sourced from Deiters, A., Groff, D., Ryu, Y., Xie, J. and Schultz, P. G. (2006), A Genetically Encoded Photocaged Tyrosine. Angew. Chem. Int. Ed., 45: 2728???2731.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: ONBYRS promoter
sequence annotation: ONBYRS
sequence annotation: terminator
sequence annotation: tRNA
sequence annotation: tRNA promoter
sequence: https://synbiohub.org/public/igem/BBa_K1416000_sequence/1

ONBYRS promoter
type: SequenceAnnotation
persistent identity: annotation2426651
display id: annotation2426651
version: 1
top level: ONBY RS
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1416000/annotation2426651/range2426651/1

https://synbiohub.org/public/igem/BBa_K1416000/annotation2426651/range2426651/1
type: Range
persistent identity: range2426651
display id: range2426651
version: 1
direction: direction/forward
top level: ONBY RS
start: 72
end: 219
orientation: inline

ONBYRS
type: SequenceAnnotation
persistent identity: annotation2426652
display id: annotation2426652
version: 1
top level: ONBY RS
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1416000/annotation2426652/range2426652/1

https://synbiohub.org/public/igem/BBa_K1416000/annotation2426652/range2426652/1
type: Range
persistent identity: range2426652
display id: range2426652
version: 1
direction: direction/forward
top level: ONBY RS
start: 233
end: 1153
orientation: inline

terminator
type: SequenceAnnotation
persistent identity: annotation2426655
display id: annotation2426655
version: 1
top level: ONBY RS
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1416000/annotation2426655/range2426655/1

https://synbiohub.org/public/igem/BBa_K1416000/annotation2426655/range2426655/1
type: Range
persistent identity: range2426655
display id: range2426655
version: 1
direction: direction/reverse
top level: ONBY RS
start: 1497
end: 1526
orientation: inline

tRNA
type: SequenceAnnotation
persistent identity: annotation2426654
display id: annotation2426654
version: 1
top level: ONBY RS
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1416000/annotation2426654/range2426654/1

https://synbiohub.org/public/igem/BBa_K1416000/annotation2426654/range2426654/1
type: Range
persistent identity: range2426654
display id: range2426654
version: 1
direction: direction/forward
top level: ONBY RS
start: 1396
end: 1471
orientation: inline

tRNA promoter
type: SequenceAnnotation
persistent identity: annotation2426653
display id: annotation2426653
version: 1
top level: ONBY RS
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1416000/annotation2426653/range2426653/1

https://synbiohub.org/public/igem/BBa_K1416000/annotation2426653/range2426653/1
type: Range
persistent identity: range2426653
display id: range2426653
version: 1
direction: direction/forward
top level: ONBY RS
start: 1330
end: 1379
orientation: inline

SspGyrB(C)
type: ComponentDefinition
persistent identity: BBa K1362121
display id: BBa_K1362121
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS + SspGyrB C-intein RFC[105] assembly construct (with His6)
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B??scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch??fer, Carolin Schmelas, Silvan Schmitz, Max Waldha
created: 2014-10-08T11:00:00Z
modified: 2015-05-08T01:10:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1738_
m user id: 0
owner id: 12377
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: ???
mutable notes: ???
mutable provenance: ???
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1362465
component: BBa_K1362414
component: BBa_K1362422
component: BBa_K1362407
component: BBa_K1362421
component: BBa_K1362467
component: BBa_K1362090
component: BBa_G0000
component: BBa_K1362418
component: BBa_K1362431
component: BBa_K1362419
sequence annotation: BBa_K1362418
sequence annotation: BBa_K1362465
sequence annotation: BBa_K1362431
sequence annotation: BBa_K1362467
sequence annotation: BBa_K1362419
sequence annotation: BBa_K1362422
sequence annotation: BBa_K1362407
sequence annotation: BBa_K1362414
sequence annotation: BBa_G0000
sequence annotation: BBa_K1362090
sequence annotation: BBa_K1362421
sequence: https://synbiohub.org/public/igem/BBa_K1362121_sequence/1

BBa_K1362465
type: Component
persistent identity: component2410468
display id: component2410468
version: 1
top level: SspGyrB(C)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362465/1

BBa_K1362414
type: Component
persistent identity: component2410437
display id: component2410437
version: 1
top level: SspGyrB(C)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362414/1

BBa_K1362422
type: Component
persistent identity: component2410465
display id: component2410465
version: 1
top level: SspGyrB(C)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362422/1

BBa_K1362407
type: Component
persistent identity: component2410443
display id: component2410443
version: 1
top level: SspGyrB(C)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362407/1

BBa_K1362421
type: Component
persistent identity: component2410467
display id: component2410467
version: 1
top level: SspGyrB(C)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362421/1

BBa_K1362467
type: Component
persistent identity: component2410442
display id: component2410442
version: 1
top level: SspGyrB(C)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362467/1

BBa_K1362090
type: Component
persistent identity: component2410435
display id: component2410435
version: 1
top level: SspGyrB(C)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362090/1

BBa_G0000
type: Component
persistent identity: component2410436
display id: component2410436
version: 1
top level: SspGyrB(C)
access: public
definition: https://synbiohub.org/public/igem/BBa_G0000/1

BBa_K1362418
type: Component
persistent identity: component2410441
display id: component2410441
version: 1
top level: SspGyrB(C)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362418/1

BBa_K1362431
type: Component
persistent identity: component2410440
display id: component2410440
version: 1
top level: SspGyrB(C)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362431/1

BBa_K1362419
type: Component
persistent identity: component2410444
display id: component2410444
version: 1
top level: SspGyrB(C)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362419/1

BBa_K1362418
type: SequenceAnnotation
persistent identity: annotation2410441
display id: annotation2410441
version: 1
top level: SspGyrB(C)
location: https://synbiohub.org/public/igem/BBa_K1362121/annotation2410441/range2410441/1
component: BBa_K1362418

https://synbiohub.org/public/igem/BBa_K1362121/annotation2410441/range2410441/1
type: Range
persistent identity: range2410441
display id: range2410441
version: 1
direction: direction/reverse
top level: SspGyrB(C)
start: 60
end: 63
orientation: inline

BBa_K1362465
type: SequenceAnnotation
persistent identity: annotation2410468
display id: annotation2410468
version: 1
top level: SspGyrB(C)
location: https://synbiohub.org/public/igem/BBa_K1362121/annotation2410468/range2410468/1
component: BBa_K1362465

https://synbiohub.org/public/igem/BBa_K1362121/annotation2410468/range2410468/1
type: Range
persistent identity: range2410468
display id: range2410468
version: 1
direction: direction/reverse
top level: SspGyrB(C)
start: 1171
end: 1172
orientation: inline

BBa_K1362431
type: SequenceAnnotation
persistent identity: annotation2410440
display id: annotation2410440
version: 1
top level: SspGyrB(C)
location: https://synbiohub.org/public/igem/BBa_K1362121/annotation2410440/range2410440/1
component: BBa_K1362431

https://synbiohub.org/public/igem/BBa_K1362121/annotation2410440/range2410440/1
type: Range
persistent identity: range2410440
display id: range2410440
version: 1
direction: direction/reverse
top level: SspGyrB(C)
start: 39
end: 59
orientation: inline

BBa_K1362467
type: SequenceAnnotation
persistent identity: annotation2410442
display id: annotation2410442
version: 1
top level: SspGyrB(C)
location: https://synbiohub.org/public/igem/BBa_K1362121/annotation2410442/range2410442/1
component: BBa_K1362467

https://synbiohub.org/public/igem/BBa_K1362121/annotation2410442/range2410442/1
type: Range
persistent identity: range2410442
display id: range2410442
version: 1
direction: direction/reverse
top level: SspGyrB(C)
start: 64
end: 65
orientation: inline

BBa_K1362419
type: SequenceAnnotation
persistent identity: annotation2410444
display id: annotation2410444
version: 1
top level: SspGyrB(C)
location: https://synbiohub.org/public/igem/BBa_K1362121/annotation2410444/range2410444/1
component: BBa_K1362419

https://synbiohub.org/public/igem/BBa_K1362121/annotation2410444/range2410444/1
type: Range
persistent identity: range2410444
display id: range2410444
version: 1
direction: direction/reverse
top level: SspGyrB(C)
start: 80
end: 83
orientation: inline

BBa_K1362422
type: SequenceAnnotation
persistent identity: annotation2410465
display id: annotation2410465
version: 1
top level: SspGyrB(C)
location: https://synbiohub.org/public/igem/BBa_K1362121/annotation2410465/range2410465/1
component: BBa_K1362422

https://synbiohub.org/public/igem/BBa_K1362121/annotation2410465/range2410465/1
type: Range
persistent identity: range2410465
display id: range2410465
version: 1
direction: direction/reverse
top level: SspGyrB(C)
start: 84
end: 1166
orientation: inline

BBa_K1362407
type: SequenceAnnotation
persistent identity: annotation2410443
display id: annotation2410443
version: 1
top level: SspGyrB(C)
location: https://synbiohub.org/public/igem/BBa_K1362121/annotation2410443/range2410443/1
component: BBa_K1362407

https://synbiohub.org/public/igem/BBa_K1362121/annotation2410443/range2410443/1
type: Range
persistent identity: range2410443
display id: range2410443
version: 1
direction: direction/reverse
top level: SspGyrB(C)
start: 66
end: 79
orientation: inline

BBa_K1362414
type: SequenceAnnotation
persistent identity: annotation2410437
display id: annotation2410437
version: 1
top level: SspGyrB(C)
location: https://synbiohub.org/public/igem/BBa_K1362121/annotation2410437/range2410437/1
component: BBa_K1362414

https://synbiohub.org/public/igem/BBa_K1362121/annotation2410437/range2410437/1
type: Range
persistent identity: range2410437
display id: range2410437
version: 1
direction: direction/reverse
top level: SspGyrB(C)
start: 36
end: 38
orientation: inline

BBa_G0000
type: SequenceAnnotation
persistent identity: annotation2410436
display id: annotation2410436
version: 1
top level: SspGyrB(C)
location: https://synbiohub.org/public/igem/BBa_K1362121/annotation2410436/range2410436/1
component: BBa_G0000

https://synbiohub.org/public/igem/BBa_K1362121/annotation2410436/range2410436/1
type: Range
persistent identity: range2410436
display id: range2410436
version: 1
direction: direction/reverse
top level: SspGyrB(C)
start: 30
end: 35
orientation: inline

BBa_K1362090
type: SequenceAnnotation
persistent identity: annotation2410435
display id: annotation2410435
version: 1
top level: SspGyrB(C)
location: https://synbiohub.org/public/igem/BBa_K1362121/annotation2410435/range2410435/1
component: BBa_K1362090

https://synbiohub.org/public/igem/BBa_K1362121/annotation2410435/range2410435/1
type: Range
persistent identity: range2410435
display id: range2410435
version: 1
direction: direction/reverse
top level: SspGyrB(C)
start: 1
end: 29
orientation: inline

BBa_K1362421
type: SequenceAnnotation
persistent identity: annotation2410467
display id: annotation2410467
version: 1
top level: SspGyrB(C)
location: https://synbiohub.org/public/igem/BBa_K1362121/annotation2410467/range2410467/1
component: BBa_K1362421

https://synbiohub.org/public/igem/BBa_K1362121/annotation2410467/range2410467/1
type: Range
persistent identity: range2410467
display id: range2410467
version: 1
direction: direction/reverse
top level: SspGyrB(C)
start: 1167
end: 1170
orientation: inline

BBa_K1362202
type: ComponentDefinition
persistent identity: BBa K1362202
display id: BBa_K1362202
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Sortase A circularization construct (with His6; without RBS)
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Büscher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schäfer, Carolin Schmelas, Silvan Schmitz, Max Waldha
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:05Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1738_
m user id: 0
owner id: 22951
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: TODO
mutable notes: TODO
mutable provenance: TODO
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1362457
component: BBa_J04450
component: BBa_K1362427
component: BBa_K1362424
component: BBa_J70594
component: BBa_K1362452
component: BBa_K1362454
component: BBa_K1362445
sequence annotation: BBa_K1362454
sequence annotation: BBa_K1362424
sequence annotation: BBa_K1362445
sequence annotation: BBa_K1362427
sequence annotation: BBa_K1362457
sequence annotation: BBa_J70594
sequence annotation: BBa_J04450
sequence annotation: BBa_K1362452
sequence: https://synbiohub.org/public/igem/BBa_K1362202_sequence/1

BBa_K1362457
type: Component
persistent identity: component2410787
display id: component2410787
version: 1
top level: BBa_K1362202
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362457/1

BBa_J04450
type: Component
persistent identity: component2410784
display id: component2410784
version: 1
top level: BBa_K1362202
access: public
definition: BBa_J04450

BBa_K1362427
type: Component
persistent identity: component2410766
display id: component2410766
version: 1
top level: BBa_K1362202
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362427/1

BBa_K1362424
type: Component
persistent identity: component2410785
display id: component2410785
version: 1
top level: BBa_K1362202
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362424/1

BBa_J70594
type: Component
persistent identity: component2410789
display id: component2410789
version: 1
top level: BBa_K1362202
access: public
definition: https://synbiohub.org/public/igem/BBa_J70594/1

BBa_K1362452
type: Component
persistent identity: component2410765
display id: component2410765
version: 1
top level: BBa_K1362202
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362452/1

BBa_K1362454
type: Component
persistent identity: component2410786
display id: component2410786
version: 1
top level: BBa_K1362202
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362454/1

BBa_K1362445
type: Component
persistent identity: component2410764
display id: component2410764
version: 1
top level: BBa_K1362202
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362445/1

BBa_K1362454
type: SequenceAnnotation
persistent identity: annotation2410786
display id: annotation2410786
version: 1
top level: BBa_K1362202
location: https://synbiohub.org/public/igem/BBa_K1362202/annotation2410786/range2410786/1
component: BBa_K1362454

https://synbiohub.org/public/igem/BBa_K1362202/annotation2410786/range2410786/1
type: Range
persistent identity: range2410786
display id: range2410786
version: 1
direction: direction/reverse
top level: BBa_K1362202
start: 1116
end: 1133
orientation: inline

BBa_K1362424
type: SequenceAnnotation
persistent identity: annotation2410785
display id: annotation2410785
version: 1
top level: BBa_K1362202
location: https://synbiohub.org/public/igem/BBa_K1362202/annotation2410785/range2410785/1
component: BBa_K1362424

https://synbiohub.org/public/igem/BBa_K1362202/annotation2410785/range2410785/1
type: Range
persistent identity: range2410785
display id: range2410785
version: 1
direction: direction/reverse
top level: BBa_K1362202
start: 1109
end: 1115
orientation: inline

BBa_K1362445
type: SequenceAnnotation
persistent identity: annotation2410764
display id: annotation2410764
version: 1
top level: BBa_K1362202
location: https://synbiohub.org/public/igem/BBa_K1362202/annotation2410764/range2410764/1
component: BBa_K1362445

https://synbiohub.org/public/igem/BBa_K1362202/annotation2410764/range2410764/1
type: Range
persistent identity: range2410764
display id: range2410764
version: 1
direction: direction/reverse
top level: BBa_K1362202
start: 1
end: 12
orientation: inline

BBa_K1362427
type: SequenceAnnotation
persistent identity: annotation2410766
display id: annotation2410766
version: 1
top level: BBa_K1362202
location: https://synbiohub.org/public/igem/BBa_K1362202/annotation2410766/range2410766/1
component: BBa_K1362427

https://synbiohub.org/public/igem/BBa_K1362202/annotation2410766/range2410766/1
type: Range
persistent identity: range2410766
display id: range2410766
version: 1
direction: direction/reverse
top level: BBa_K1362202
start: 34
end: 39
orientation: inline

BBa_K1362457
type: SequenceAnnotation
persistent identity: annotation2410787
display id: annotation2410787
version: 1
top level: BBa_K1362202
location: https://synbiohub.org/public/igem/BBa_K1362202/annotation2410787/range2410787/1
component: BBa_K1362457

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type: Range
persistent identity: range2410787
display id: range2410787
version: 1
direction: direction/reverse
top level: BBa_K1362202
start: 1134
end: 1151
orientation: inline

BBa_J70594
type: SequenceAnnotation
persistent identity: annotation2410789
display id: annotation2410789
version: 1
top level: BBa_K1362202
location: https://synbiohub.org/public/igem/BBa_K1362202/annotation2410789/range2410789/1
component: BBa_J70594

https://synbiohub.org/public/igem/BBa_K1362202/annotation2410789/range2410789/1
type: Range
persistent identity: range2410789
display id: range2410789
version: 1
direction: direction/reverse
top level: BBa_K1362202
start: 1152
end: 1157
orientation: inline

BBa_J04450
type: SequenceAnnotation
persistent identity: annotation2410784
display id: annotation2410784
version: 1
top level: BBa_K1362202
location: https://synbiohub.org/public/igem/BBa_K1362202/annotation2410784/range2410784/1
component: BBa_J04450

https://synbiohub.org/public/igem/BBa_K1362202/annotation2410784/range2410784/1
type: Range
persistent identity: range2410784
display id: range2410784
version: 1
direction: direction/reverse
top level: BBa_K1362202
start: 40
end: 1108
orientation: inline

BBa_K1362452
type: SequenceAnnotation
persistent identity: annotation2410765
display id: annotation2410765
version: 1
top level: BBa_K1362202
location: https://synbiohub.org/public/igem/BBa_K1362202/annotation2410765/range2410765/1
component: BBa_K1362452

https://synbiohub.org/public/igem/BBa_K1362202/annotation2410765/range2410765/1
type: Range
persistent identity: range2410765
display id: range2410765
version: 1
direction: direction/reverse
top level: BBa_K1362202
start: 13
end: 33
orientation: inline

BBa_K1343021
type: ComponentDefinition
persistent identity: BBa K1343021
display id: BBa_K1343021
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Plux->amilCP->lambda CI
creator: Tal Naor, Ricarina Rabinovitz, Ittai Rubinstein, Yair Maimon
created: 2014-10-05T11:00:00Z
modified: 2015-05-08T01:10:00Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1718_
m user id: 0
owner id: 20669
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: The promoter Plux in induced by the dimer AHL-luxR. As a result, amilCP, a blue chromophoire protein, is synthesized. Lambda phage CI is a repressor of the promoter PCI.
mutable notes: r
mutable provenance: BBa_R0062: Plux, BBa_B0030: Strong RBS, BBa_K592009: amilCP, BBa_B0010:T1 (terminator) from E. coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K1343021_sequence/1

BBa_K1315001
type: ComponentDefinition
persistent identity: BBa K1315001
display id: BBa_K1315001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: The pqsA promoter from Pseudomonas
creator: Dimitrios Michailidis
created: 2014-09-19T11:00:00Z
modified: 2015-05-08T01:09:48Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1690_
m user id: 0
owner id: 21812
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: The Pseudomonas quinolone signal (PQS) is imperative in modulating mechanisms that enable Pseudomonas aeruginosa to survive at times of stress. P. aeruginosa is the main pathogen responsible for severe deterioration in the Cystic Fibrosis lungs. The production of this signal relies on the pqsABCDE operon. The transcriptional factor PqsR (BBa_K1315000) positively regulates this operon. http://www.ncbi.nlm.nih.gov/pubmed/25225275 http://www.ncbi.nlm.nih.gov/pubmed/16735731 http://www.ncbi.nlm.nih.gov/pubmed/21539583 http://www.ncbi.nlm.nih.gov/pubmed/?term=pqsr-dependent+and+pqsr-independent+regulation+of+motility+and+biofilm+formation+by+pqs http://www.ncbi.nlm.nih.gov/pubmed/12393198
mutable notes: The sequence did not have any restriction sites to remove so it was immediately PCRed out of the genome and had the registry prefix and suffix added to it. It was then ligated into the pSB1C3 vector.
mutable provenance: The source of this part is the genome of Pseudomonas aeruginosa PAO1 provided by the Robert Ryan lab in the Molecular Microbiology department of the University of Dundee, UK.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: pqsABCDE
sequence: https://synbiohub.org/public/igem/BBa_K1315001_sequence/1

pqsABCDE
type: SequenceAnnotation
persistent identity: annotation2384758
display id: annotation2384758
version: 1
top level: BBa_K1315001
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1315001/annotation2384758/range2384758/1

https://synbiohub.org/public/igem/BBa_K1315001/annotation2384758/range2384758/1
type: Range
persistent identity: range2384758
display id: range2384758
version: 1
direction: direction/forward
top level: BBa_K1315001
start: 1
end: 92
orientation: inline

BBa_K1763424
type: ComponentDefinition
persistent identity: BBa K1763424
display id: BBa_K1763424
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: MaSp2-3(1C3)
creator: Vinson Lam
created: 2015-09-14T11:00:00Z
modified: 2015-09-16T11:06:16Z
discontinued: false
dominant: false
group u list: _2185_
m user id: 27136
owner id: 27136
owning group id: 9
status: status/Planning
bookmark: false
mutable description: 3-mer construct of MaSp2 only.
mutable notes: Assembled using ICA
mutable provenance: Nephila clavipes
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: 8-his tag
sequence annotation: M2-CA
sequence annotation: M2-AB
sequence annotation: M2-BC
sequence: https://synbiohub.org/public/igem/BBa_K1763424_sequence/1

8-his tag
type: SequenceAnnotation
persistent identity: annotation2463797
display id: annotation2463797
version: 1
top level: BBa_K1763424
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1763424/annotation2463797/range2463797/1

https://synbiohub.org/public/igem/BBa_K1763424/annotation2463797/range2463797/1
type: Range
persistent identity: range2463797
display id: range2463797
version: 1
direction: direction/forward
top level: BBa_K1763424
start: 4
end: 27
orientation: inline

M2-CA
type: SequenceAnnotation
persistent identity: annotation2463823
display id: annotation2463823
version: 1
top level: BBa_K1763424
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763424/annotation2463823/range2463823/1

https://synbiohub.org/public/igem/BBa_K1763424/annotation2463823/range2463823/1
type: Range
persistent identity: range2463823
display id: range2463823
version: 1
direction: direction/forward
top level: BBa_K1763424
start: 240
end: 341
orientation: inline

M2-AB
type: SequenceAnnotation
persistent identity: annotation2463808
display id: annotation2463808
version: 1
top level: BBa_K1763424
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763424/annotation2463808/range2463808/1

https://synbiohub.org/public/igem/BBa_K1763424/annotation2463808/range2463808/1
type: Range
persistent identity: range2463808
display id: range2463808
version: 1
direction: direction/forward
top level: BBa_K1763424
start: 36
end: 137
orientation: inline

M2-BC
type: SequenceAnnotation
persistent identity: annotation2463818
display id: annotation2463818
version: 1
top level: BBa_K1763424
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763424/annotation2463818/range2463818/1

https://synbiohub.org/public/igem/BBa_K1763424/annotation2463818/range2463818/1
type: Range
persistent identity: range2463818
display id: range2463818
version: 1
direction: direction/forward
top level: BBa_K1763424
start: 138
end: 239
orientation: inline

BBa_K1689006
type: ComponentDefinition
persistent identity: BBa K1689006
display id: BBa_K1689006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Coding sequence of FKBP-Cluc394
creator: Xiang Li
created: 2015-09-15T11:00:00Z
modified: 2016-01-19T01:28:16Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2107_
m user id: 4206
owner id: 26464
owning group id: 9
status: status/Planning
bookmark: false
mutable description: including FKBP, 2??linker, and firefly luciferase C terminal 394 amino acid domain.
mutable notes: FKBP and C terminal 394 amino acid domain are linked by 2??linker
mutable provenance: E.coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: stop codon
sequence annotation: GGGGSGGGGS linker
sequence annotation: FKBP
sequence annotation: start codon
sequence annotation: C394 terminal domain of firefly luciferase
sequence annotation: J23110
sequence annotation: B0034
sequence: https://synbiohub.org/public/igem/BBa_K1689006_sequence/1

stop codon
type: SequenceAnnotation
persistent identity: annotation2470155
display id: annotation2470155
version: 1
top level: BBa_K1689006
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1689006/annotation2470155/range2470155/1

https://synbiohub.org/public/igem/BBa_K1689006/annotation2470155/range2470155/1
type: Range
persistent identity: range2470155
display id: range2470155
version: 1
direction: direction/forward
top level: BBa_K1689006
start: 873
end: 875
orientation: inline

GGGGSGGGGS linker
type: SequenceAnnotation
persistent identity: annotation2470152
display id: annotation2470152
version: 1
top level: BBa_K1689006
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1689006/annotation2470152/range2470152/1

https://synbiohub.org/public/igem/BBa_K1689006/annotation2470152/range2470152/1
type: Range
persistent identity: range2470152
display id: range2470152
version: 1
direction: direction/forward
top level: BBa_K1689006
start: 372
end: 401
orientation: inline

FKBP
type: SequenceAnnotation
persistent identity: annotation2470150
display id: annotation2470150
version: 1
top level: BBa_K1689006
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1689006/annotation2470150/range2470150/1

https://synbiohub.org/public/igem/BBa_K1689006/annotation2470150/range2470150/1
type: Range
persistent identity: range2470150
display id: range2470150
version: 1
direction: direction/forward
top level: BBa_K1689006
start: 51
end: 371
orientation: inline

start codon
type: SequenceAnnotation
persistent identity: annotation2470149
display id: annotation2470149
version: 1
top level: BBa_K1689006
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1689006/annotation2470149/range2470149/1

https://synbiohub.org/public/igem/BBa_K1689006/annotation2470149/range2470149/1
type: Range
persistent identity: range2470149
display id: range2470149
version: 1
direction: direction/forward
top level: BBa_K1689006
start: 48
end: 50
orientation: inline

C394 terminal domain of firefly luciferase
type: SequenceAnnotation
persistent identity: annotation2470154
display id: annotation2470154
version: 1
top level: BBa_K1689006
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1689006/annotation2470154/range2470154/1

https://synbiohub.org/public/igem/BBa_K1689006/annotation2470154/range2470154/1
type: Range
persistent identity: range2470154
display id: range2470154
version: 1
direction: direction/forward
top level: BBa_K1689006
start: 402
end: 872
orientation: inline

J23110
type: SequenceAnnotation
persistent identity: annotation2470146
display id: annotation2470146
version: 1
top level: BBa_K1689006
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1689006/annotation2470146/range2470146/1

https://synbiohub.org/public/igem/BBa_K1689006/annotation2470146/range2470146/1
type: Range
persistent identity: range2470146
display id: range2470146
version: 1
direction: direction/forward
top level: BBa_K1689006
start: 1
end: 35
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2470147
display id: annotation2470147
version: 1
top level: BBa_K1689006
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1689006/annotation2470147/range2470147/1

https://synbiohub.org/public/igem/BBa_K1689006/annotation2470147/range2470147/1
type: Range
persistent identity: range2470147
display id: range2470147
version: 1
direction: direction/forward
top level: BBa_K1689006
start: 36
end: 47
orientation: inline

BBa_K1351000
type: ComponentDefinition
persistent identity: BBa K1351000
display id: BBa_K1351000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: beta-Neurexin-derived peptide binding to S. aureus adhesin SdrC
creator: Mona Dotzler
created: 2014-09-14T11:00:00Z
modified: 2015-06-01T12:53:55Z
discontinued: false
dominant: false
group u list: _1726_
m user id: 4206
owner id: 20048
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Short peptide of 11 amino acids corresponding to the N-terminus of the human protein neurexin beta-1. It includes the binding site (HHIHHFH) for the Staphylococcus aureus adhesin SdrC. Displayed on the surface of Bacillus subtilis, it shoud enable B. subtilis to bind specifically to S. aureus.
mutable notes: Oligos were designed as precut by EcoRI and PstI to shorten the synthesized fragments and to simplify the cloning into pSB1C3.
mutable provenance: The part was generated by annealing of artificially synthesized oligonucletides: Nrx1b_ENX_SD_Ngo_precutEP_fwd: AATTCgcggccgctTCTAGAgtaaggaggaaGCCGGCATG GCGCACCACATTCACCACTTTCACGGATCA ACCGGTtaatACTAGTagcggccgCTGCA Nrx1b_SNP_Age_precutEP_rev: GcggccgctACTAGTattaACCGGT TGATCCGTGAAAGTGGTGAATGTGGTGCGC CATGCCGGCttcctccttacTCTAGAagcggccgcG
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1351000_sequence/1

eYFP_GBDli
type: ComponentDefinition
persistent identity: BBa K1602037
display id: BBa_K1602037
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: eYFP with C-terminal GBD ligand and RBS BBa_B0034
creator: Jasmin Weber, Michelle Zoeller, Marcus Geissler, Stefan Bohn, Simon Linder, Sebastian Barthel
created: 2015-09-16T11:00:00Z
modified: 2015-09-17T05:05:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2019_
m user id: 22022
owner id: 22022
owning group id: 9
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K1602037_sequence/1

BBa_K1467204
type: ComponentDefinition
persistent identity: BBa K1467204
display id: BBa_K1467204
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GoldenGate compatible Green Flourescent Protein (GFP)
creator: Cara Deal
created: 2014-09-23T11:00:00Z
modified: 2015-06-01T07:05:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1846_
m user id: 4206
owner id: 21550
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: A variation of the part BBa_E0040 that has been made compatible for Golden Gate by removal of internal BsaI and BpiI recognition sequences. Fluoresces under UV light when the gene is expressed.
mutable notes: Domestication for GoldenGate by removal of internal BsaI and BpiI recognition sequences.
mutable provenance: Aequorea victoria
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K1467204_sequence/1

BBa_K1402008
type: ComponentDefinition
persistent identity: BBa K1402008
display id: BBa_K1402008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: FadD Promoter
creator: Chen Xi
created: 2014-09-28T11:00:00Z
modified: 2015-05-08T01:10:16Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1780_
m user id: 0
owner id: 16730
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part is sensitive to long chain acyl-CoA ,with a FadR binding site. In the absence of long chain acyl-CoA, FadR represses the transcription. In the presence of long chain acyl-CoA, FadR dissociates from the binding site so that the transcription begins. E.coli uses this promoter to regulate fatty acid metabolism.
mutable notes: no
mutable provenance: E.coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K1402008_sequence/1

BBa_K1602056
type: ComponentDefinition
persistent identity: BBa K1602056
display id: BBa_K1602056
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0034-yjhG-B0034-yqhD
creator: Leon Kraus, Jonathan Pletzer-Zelgert, Jonas Sindlinger, Carmen Sakas Gandullo, Sebastian Holzner, Elena Tonchevska, Mirjam Haun
created: 2015-09-15T11:00:00Z
modified: 2015-09-18T06:35:45Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2019_
m user id: 26220
owner id: 25990
owning group id: 9
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1602013
component: BBa_K1602012
component: BBa_B0034
sequence annotation: BBa_K1602012
sequence annotation: BBa_B0034
sequence annotation: BBa_K1602013
sequence: https://synbiohub.org/public/igem/BBa_K1602056_sequence/1

BBa_K1602013
type: Component
persistent identity: component2462945
display id: component2462945
version: 1
top level: BBa_K1602056
access: public
definition: BBa_K1602013

BBa_K1602012
type: Component
persistent identity: component2462942
display id: component2462942
version: 1
top level: BBa_K1602056
access: public
definition: BBa_K1602012

BBa_B0034
type: Component
persistent identity: component2462944
display id: component2462944
version: 1
top level: BBa_K1602056
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: Component
persistent identity: component2462941
display id: component2462941
version: 1
top level: BBa_K1602056
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1602012
type: SequenceAnnotation
persistent identity: annotation2462942
display id: annotation2462942
version: 1
top level: BBa_K1602056
location: https://synbiohub.org/public/igem/BBa_K1602056/annotation2462942/range2462942/1
component: BBa_K1602012

https://synbiohub.org/public/igem/BBa_K1602056/annotation2462942/range2462942/1
type: Range
persistent identity: range2462942
display id: range2462942
version: 1
direction: direction/reverse
top level: BBa_K1602056
start: 19
end: 1986
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2462941
display id: annotation2462941
version: 1
top level: BBa_K1602056
location: https://synbiohub.org/public/igem/BBa_K1602056/annotation2462941/range2462941/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1602056/annotation2462941/range2462941/1
type: Range
persistent identity: range2462941
display id: range2462941
version: 1
direction: direction/reverse
top level: BBa_K1602056
start: 1
end: 12
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2462944
display id: annotation2462944
version: 1
top level: BBa_K1602056
location: https://synbiohub.org/public/igem/BBa_K1602056/annotation2462944/range2462944/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1602056/annotation2462944/range2462944/1
type: Range
persistent identity: range2462944
display id: range2462944
version: 1
direction: direction/reverse
top level: BBa_K1602056
start: 1995
end: 2006
orientation: inline

BBa_K1602013
type: SequenceAnnotation
persistent identity: annotation2462945
display id: annotation2462945
version: 1
top level: BBa_K1602056
location: https://synbiohub.org/public/igem/BBa_K1602056/annotation2462945/range2462945/1
component: BBa_K1602013

https://synbiohub.org/public/igem/BBa_K1602056/annotation2462945/range2462945/1
type: Range
persistent identity: range2462945
display id: range2462945
version: 1
direction: direction/reverse
top level: BBa_K1602056
start: 2013
end: 3176
orientation: inline

DsbDspB
type: ComponentDefinition
persistent identity: BBa K1659201
display id: BBa_K1659201
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Dispersin B fused at N-terminal with DsbA 2-19 signal sequence
creator: Wei Chung Kong
created: 2015-08-27T11:00:00Z
modified: 2015-09-14T03:05:35Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2077_
m user id: 24524
owner id: 24524
owning group id: 9
status: status/Planning
bookmark: false
mutable description: to be updated
mutable notes: to be updated
mutable provenance: to be updated
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: Hisx6
sequence annotation: DsbA 2-19 Signal Sequence
sequence annotation: stop
sequence annotation: DspB
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_K1659201_sequence/1

Hisx6
type: SequenceAnnotation
persistent identity: annotation2449116
display id: annotation2449116
version: 1
top level: DsbDspB
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1659201/annotation2449116/range2449116/1

https://synbiohub.org/public/igem/BBa_K1659201/annotation2449116/range2449116/1
type: Range
persistent identity: range2449116
display id: range2449116
version: 1
direction: direction/forward
top level: DsbDspB
start: 1141
end: 1158
orientation: inline

DsbA 2-19 Signal Sequence
type: SequenceAnnotation
persistent identity: annotation2449114
display id: annotation2449114
version: 1
top level: DsbDspB
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1659201/annotation2449114/range2449114/1

https://synbiohub.org/public/igem/BBa_K1659201/annotation2449114/range2449114/1
type: Range
persistent identity: range2449114
display id: range2449114
version: 1
direction: direction/forward
top level: DsbDspB
start: 4
end: 57
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2453897
display id: annotation2453897
version: 1
top level: DsbDspB
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1659201/annotation2453897/range2453897/1

https://synbiohub.org/public/igem/BBa_K1659201/annotation2453897/range2453897/1
type: Range
persistent identity: range2453897
display id: range2453897
version: 1
direction: direction/forward
top level: DsbDspB
start: 1159
end: 1164
orientation: inline

DspB
type: SequenceAnnotation
persistent identity: annotation2449115
display id: annotation2449115
version: 1
top level: DsbDspB
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1659201/annotation2449115/range2449115/1

https://synbiohub.org/public/igem/BBa_K1659201/annotation2449115/range2449115/1
type: Range
persistent identity: range2449115
display id: range2449115
version: 1
direction: direction/forward
top level: DsbDspB
start: 58
end: 1140
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2449113
display id: annotation2449113
version: 1
top level: DsbDspB
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1659201/annotation2449113/range2449113/1

https://synbiohub.org/public/igem/BBa_K1659201/annotation2449113/range2449113/1
type: Range
persistent identity: range2449113
display id: range2449113
version: 1
direction: direction/forward
top level: DsbDspB
start: 1
end: 3
orientation: inline

BBa_K1640020
type: ComponentDefinition
persistent identity: BBa K1640020
display id: BBa_K1640020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: psbA
creator: Meghan Cook
created: 2015-09-09T11:00:00Z
modified: 2015-09-18T08:54:57Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2057_
m user id: 28565
owner id: 28565
owning group id: 9
status: status/Planning
bookmark: true
mutable description: n/a
mutable notes: n/a
mutable provenance: n/a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: double terminator
sequence annotation: rbs
sequence annotation: psbA
sequence: https://synbiohub.org/public/igem/BBa_K1640020_sequence/1

double terminator
type: SequenceAnnotation
persistent identity: annotation2448253
display id: annotation2448253
version: 1
top level: BBa_K1640020
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1640020/annotation2448253/range2448253/1

https://synbiohub.org/public/igem/BBa_K1640020/annotation2448253/range2448253/1
type: Range
persistent identity: range2448253
display id: range2448253
version: 1
direction: direction/forward
top level: BBa_K1640020
start: 1075
end: 1203
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2448251
display id: annotation2448251
version: 1
top level: BBa_K1640020
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1640020/annotation2448251/range2448251/1

https://synbiohub.org/public/igem/BBa_K1640020/annotation2448251/range2448251/1
type: Range
persistent identity: range2448251
display id: range2448251
version: 1
direction: direction/forward
top level: BBa_K1640020
start: 1
end: 15
orientation: inline

psbA
type: SequenceAnnotation
persistent identity: annotation2448252
display id: annotation2448252
version: 1
top level: BBa_K1640020
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1640020/annotation2448252/range2448252/1

https://synbiohub.org/public/igem/BBa_K1640020/annotation2448252/range2448252/1
type: Range
persistent identity: range2448252
display id: range2448252
version: 1
direction: direction/forward
top level: BBa_K1640020
start: 16
end: 1074
orientation: inline

BBa_K1604022
type: ComponentDefinition
persistent identity: BBa K1604022
display id: BBa_K1604022
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: araC-pBAD + β-carotene + J23100 + blh
creator: Veronica Pinamonti
created: 2015-08-19T11:00:00Z
modified: 2015-09-17T05:19:54Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2021_
m user id: 11821
owner id: 24581
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This biobrick is obtained by the assembly of two parts K1604020 and K1604021. The composite part contains the five genes necessary for the retinal synthesis. The first part contains ctrEIBY under araC-pBAD, these four gene are responsive of beta-carotene production starting from FPP, a colurless precurse presents in E.coli. The second part contains blh under costitutive promoter. It encodes for the beta-carotene15-15'dioxygenase that catalizes the cleavege of a single molecule of beta-carotene into two molecules of retinal.
mutable notes: The two parts was joined together by strandard assembly, before it beta-carotene was amplified by Phusion PCR.
mutable provenance: AraC-pBAD promoter comes from E.coli genome, while ctrEIBY are from Pantoea ananatis (formerly Erwinia uredovora) genome. Blh gene and its promoter are synthetized and codon optimezed by GeneScript.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1604021
component: BBa_K1604020
sequence annotation: BBa_K1604020
sequence annotation: BBa_K1604021
sequence: https://synbiohub.org/public/igem/BBa_K1604022_sequence/1

BBa_K1604021
type: Component
persistent identity: component2449205
display id: component2449205
version: 1
top level: BBa_K1604022
access: public
definition: BBa_K1604021

BBa_K1604020
type: Component
persistent identity: component2449228
display id: component2449228
version: 1
top level: BBa_K1604022
access: public
definition: https://synbiohub.org/public/igem/BBa_K1604020/1

BBa_K1604020
type: SequenceAnnotation
persistent identity: annotation2449228
display id: annotation2449228
version: 1
top level: BBa_K1604022
location: https://synbiohub.org/public/igem/BBa_K1604022/annotation2449228/range2449228/1
component: BBa_K1604020

https://synbiohub.org/public/igem/BBa_K1604022/annotation2449228/range2449228/1
type: Range
persistent identity: range2449228
display id: range2449228
version: 1
direction: direction/reverse
top level: BBa_K1604022
start: 898
end: 6663
orientation: inline

BBa_K1604021
type: SequenceAnnotation
persistent identity: annotation2449205
display id: annotation2449205
version: 1
top level: BBa_K1604022
location: https://synbiohub.org/public/igem/BBa_K1604022/annotation2449205/range2449205/1
component: BBa_K1604021

https://synbiohub.org/public/igem/BBa_K1604022/annotation2449205/range2449205/1
type: Range
persistent identity: range2449205
display id: range2449205
version: 1
direction: direction/reverse
top level: BBa_K1604022
start: 1
end: 889
orientation: inline

BBa_K1343019
type: ComponentDefinition
persistent identity: BBa K1343019
display id: BBa_K1343019
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Plux->Green Renilla Luciferase->tetR
creator: Tal Naor, Ricarina Rabinovitz, Ittai Rubinstein, Yair Maimon
created: 2014-10-05T11:00:00Z
modified: 2015-05-08T01:10:00Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1718_
m user id: 0
owner id: 20669
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: The promoter Plux is induced by the binding of an AHL-luxR dimer. This bio-brick leads to the synthesis of the enzyme Green Renilla Luciferase, which catalyses a luminescent reaction. TetR can be used to repress the promoter Ptet.
mutable notes: r
mutable provenance: BBa_R0062: Plux, BBa_B0030: Strong RBS, BBa_B0030: Strong RBS, BBa_B0010:T1 (terminator) from E. coli.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K1343019_sequence/1

BBa_K1699004
type: ComponentDefinition
persistent identity: BBa K1699004
display id: BBa_K1699004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: gRNA for SaCas9 targeting human ubiquitin B gene under U6 promoter
creator: Emil Ruvinov
created: 2015-08-08T11:00:00Z
modified: 2015-09-13T06:26:36Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2117_
m user id: 24826
owner id: 24826
owning group id: 9
status: status/Planning
bookmark: false
mutable description: gRNA for SaCas9 targeting ubiquitin B under U6 promoter
mutable notes: Design
mutable provenance: Source
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: gRNA-UBB-Exon 2
sequence annotation: gRNA scaffold for SaCas9
sequence annotation: Human U6 promoter
sequence: https://synbiohub.org/public/igem/BBa_K1699004_sequence/1

gRNA-UBB-Exon 2
type: SequenceAnnotation
persistent identity: annotation2434619
display id: annotation2434619
version: 1
top level: BBa_K1699004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1699004/annotation2434619/range2434619/1

https://synbiohub.org/public/igem/BBa_K1699004/annotation2434619/range2434619/1
type: Range
persistent identity: range2434619
display id: range2434619
version: 1
direction: direction/forward
top level: BBa_K1699004
start: 257
end: 278
orientation: inline

gRNA scaffold for SaCas9
type: SequenceAnnotation
persistent identity: annotation2434620
display id: annotation2434620
version: 1
top level: BBa_K1699004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1699004/annotation2434620/range2434620/1

https://synbiohub.org/public/igem/BBa_K1699004/annotation2434620/range2434620/1
type: Range
persistent identity: range2434620
display id: range2434620
version: 1
direction: direction/forward
top level: BBa_K1699004
start: 279
end: 359
orientation: inline

Human U6 promoter
type: SequenceAnnotation
persistent identity: annotation2434618
display id: annotation2434618
version: 1
top level: BBa_K1699004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1699004/annotation2434618/range2434618/1

https://synbiohub.org/public/igem/BBa_K1699004/annotation2434618/range2434618/1
type: Range
persistent identity: range2434618
display id: range2434618
version: 1
direction: direction/forward
top level: BBa_K1699004
start: 1
end: 249
orientation: inline

BBa_K1696015
type: ComponentDefinition
persistent identity: BBa K1696015
display id: BBa_K1696015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Mf-ssrA Tag1 reporter
creator: Yue Liu
created: 2015-09-13T11:00:00Z
modified: 2015-09-15T09:21:00Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2114_
m user id: 16881
owner id: 16881
owning group id: 9
status: status/Planning
bookmark: false
mutable description: We incorporated tag1 into a RFP-based test platform for inducible protein degradation in E. coli.
mutable notes: codon optimization
mutable provenance: E.coli and M. florum.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence: https://synbiohub.org/public/igem/BBa_K1696015_sequence/1

BBa_K1423006
type: ComponentDefinition
persistent identity: BBa K1423006
display id: BBa_K1423006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Arsenic Inducible Banana Odor Generator
creator: Kayla DeSanty
created: 2014-09-22T11:00:00Z
modified: 2015-05-08T01:10:23Z
discontinued: false
dominant: false
group u list: _1801_
m user id: 0
owner id: 22282
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: The aresnic inducible banana odor generator converts isoamyl alcohol to isoamyl acetate in the presence of arsenic.
mutable notes: We digested the Biobricks with CIP to assure that the plasmids did not re-ligate to themselves.
mutable provenance: The arsenic promoter with the arsR repressor gene is naturally found in E. coli. The ribosome binding site, the ATF1 gene, and the double terminator combined sequence was obtained from the Biobrick BBa_J45199.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_J33201
component: BBa_J45199
sequence annotation: BBa_J45199
sequence annotation: BBa_J33201
sequence: https://synbiohub.org/public/igem/BBa_K1423006_sequence/1

BBa_J33201
type: Component
persistent identity: component2385461
display id: component2385461
version: 1
top level: BBa_K1423006
access: public
definition: https://synbiohub.org/public/igem/BBa_J33201/1

BBa_J45199
type: Component
persistent identity: component2385476
display id: component2385476
version: 1
top level: BBa_K1423006
access: public
definition: https://synbiohub.org/public/igem/BBa_J45199/1

BBa_J45199
type: SequenceAnnotation
persistent identity: annotation2385476
display id: annotation2385476
version: 1
top level: BBa_K1423006
location: https://synbiohub.org/public/igem/BBa_K1423006/annotation2385476/range2385476/1
component: BBa_J45199

https://synbiohub.org/public/igem/BBa_K1423006/annotation2385476/range2385476/1
type: Range
persistent identity: range2385476
display id: range2385476
version: 1
direction: direction/reverse
top level: BBa_K1423006
start: 527
end: 2265
orientation: inline

BBa_J33201
type: SequenceAnnotation
persistent identity: annotation2385461
display id: annotation2385461
version: 1
top level: BBa_K1423006
location: https://synbiohub.org/public/igem/BBa_K1423006/annotation2385461/range2385461/1
component: BBa_J33201

https://synbiohub.org/public/igem/BBa_K1423006/annotation2385461/range2385461/1
type: Range
persistent identity: range2385461
display id: range2385461
version: 1
direction: direction/reverse
top level: BBa_K1423006
start: 1
end: 518
orientation: inline

BBa_K1696013
type: ComponentDefinition
persistent identity: BBa K1696013
display id: BBa_K1696013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Mf-ssrA Tag1
creator: Yue Liu
created: 2015-09-13T11:00:00Z
modified: 2015-09-15T09:16:11Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2114_
m user id: 16881
owner id: 16881
owning group id: 9
status: status/Planning
bookmark: false
mutable description: The M. florum ssrA tag (mf-ssrA) is degraded by its endogenous Lon protease (mf-Lon) but not by E. coli Lon or ClpXP, and mf-Lon does not recognize or degrade ec-ssrA, providing a protease and cognate degradation tag with orthogonal functionality in E. coli.
mutable notes: codon optimization
mutable provenance: M. florum
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence: https://synbiohub.org/public/igem/BBa_K1696013_sequence/1

BBa_K1497030
type: ComponentDefinition
persistent identity: BBa K1497030
display id: BBa_K1497030
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Scaffold (S-S)
creator: Tobias Gabriel, Rene Sahm
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:46Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1877_
m user id: 0
owner id: 12466
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Scaffold (S-S)
mutable notes: Scaffold (S-S)
mutable provenance: Scaffold (S-S)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1497030_sequence/1

BBa_K1602049
type: ComponentDefinition
persistent identity: BBa K1602049
display id: BBa_K1602049
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RRkey
creator: Christian Sator,Stefan Zens, Max Zander, Benedikt Spannenkrebs, Sebastian Jaeger
created: 2015-09-15T11:00:00Z
modified: 2015-09-18T06:28:17Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2019_
m user id: 26220
owner id: 26220
owning group id: 9
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K1602049_sequence/1

nadE
type: ComponentDefinition
persistent identity: BBa K1373000
display id: BBa_K1373000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: This part encodes NAD synthetase
creator: Wenxuan Chen
created: 2014-08-13T11:00:00Z
modified: 2015-05-08T01:10:11Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1750_
m user id: 0
owner id: 23360
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: The nadE gene encodes Naphthyl Acetamide synthetase which can boot up the NAD+(H) level in the cells facilitating the electron transferring rate in MFCs (Microbial Fuel Cells).
mutable notes: The sequence is a full length ORF containing the initiation codon and the termination codon.
mutable provenance: This part is aquried from genome the E.coli K-12 substrain MG1655.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: nadE
sequence: https://synbiohub.org/public/igem/BBa_K1373000_sequence/1

nadE
type: SequenceAnnotation
persistent identity: annotation2385761
display id: annotation2385761
version: 1
top level: nadE
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1373000/annotation2385761/range2385761/1

https://synbiohub.org/public/igem/BBa_K1373000/annotation2385761/range2385761/1
type: Range
persistent identity: range2385761
display id: range2385761
version: 1
direction: direction/forward
top level: nadE
start: 1
end: 828
orientation: inline

BBa_K1632000
type: ComponentDefinition
persistent identity: BBa K1632000
display id: BBa_K1632000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: fim switch[deault ON](Tokyo_Tech/J23119)
creator: Riku Shinohara
created: 2015-08-12T11:00:00Z
modified: 2015-09-15T12:53:44Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _2049_
m user id: 24760
owner id: 22687
owning group id: 9
status: status/Planning
bookmark: false
mutable description: a
mutable notes: a
mutable provenance: a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BamHl
sequence annotation: Sall
sequence annotation: Bglll
sequence annotation: Ascl
sequence annotation: J23119 promoter
sequence annotation: Mlul
sequence: https://synbiohub.org/public/igem/BBa_K1632000_sequence/1

BamHl
type: SequenceAnnotation
persistent identity: annotation2435249
display id: annotation2435249
version: 1
top level: BBa_K1632000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1632000/annotation2435249/range2435249/1

https://synbiohub.org/public/igem/BBa_K1632000/annotation2435249/range2435249/1
type: Range
persistent identity: range2435249
display id: range2435249
version: 1
direction: direction/forward
top level: BBa_K1632000
start: 333
end: 338
orientation: inline

Sall
type: SequenceAnnotation
persistent identity: annotation2435247
display id: annotation2435247
version: 1
top level: BBa_K1632000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1632000/annotation2435247/range2435247/1

https://synbiohub.org/public/igem/BBa_K1632000/annotation2435247/range2435247/1
type: Range
persistent identity: range2435247
display id: range2435247
version: 1
direction: direction/forward
top level: BBa_K1632000
start: 319
end: 324
orientation: inline

Bglll
type: SequenceAnnotation
persistent identity: annotation2435251
display id: annotation2435251
version: 1
top level: BBa_K1632000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1632000/annotation2435251/range2435251/1

https://synbiohub.org/public/igem/BBa_K1632000/annotation2435251/range2435251/1
type: Range
persistent identity: range2435251
display id: range2435251
version: 1
direction: direction/forward
top level: BBa_K1632000
start: 374
end: 379
orientation: inline

Ascl
type: SequenceAnnotation
persistent identity: annotation2435248
display id: annotation2435248
version: 1
top level: BBa_K1632000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1632000/annotation2435248/range2435248/1

https://synbiohub.org/public/igem/BBa_K1632000/annotation2435248/range2435248/1
type: Range
persistent identity: range2435248
display id: range2435248
version: 1
direction: direction/forward
top level: BBa_K1632000
start: 325
end: 332
orientation: inline

J23119 promoter
type: SequenceAnnotation
persistent identity: annotation2435250
display id: annotation2435250
version: 1
top level: BBa_K1632000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1632000/annotation2435250/range2435250/1

https://synbiohub.org/public/igem/BBa_K1632000/annotation2435250/range2435250/1
type: Range
persistent identity: range2435250
display id: range2435250
version: 1
direction: direction/forward
top level: BBa_K1632000
start: 339
end: 373
orientation: inline

Mlul
type: SequenceAnnotation
persistent identity: annotation2435253
display id: annotation2435253
version: 1
top level: BBa_K1632000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1632000/annotation2435253/range2435253/1

https://synbiohub.org/public/igem/BBa_K1632000/annotation2435253/range2435253/1
type: Range
persistent identity: range2435253
display id: range2435253
version: 1
direction: direction/forward
top level: BBa_K1632000
start: 388
end: 392
orientation: inline

Ascl
type: SequenceAnnotation
persistent identity: annotation2435252
display id: annotation2435252
version: 1
top level: BBa_K1632000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1632000/annotation2435252/range2435252/1

https://synbiohub.org/public/igem/BBa_K1632000/annotation2435252/range2435252/1
type: Range
persistent identity: range2435252
display id: range2435252
version: 1
direction: direction/forward
top level: BBa_K1632000
start: 380
end: 387
orientation: inline

BBa_K1315009
type: ComponentDefinition
persistent identity: BBa K1315009
display id: BBa_K1315009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pseudomonas AutoInducer 1 (PAI-1) Biosensor
creator: Dimitrios Michailidis
created: 2014-09-25T11:00:00Z
modified: 2015-05-08T01:09:50Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1690_
m user id: 0
owner id: 21812
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: This is a composite part that consists of 4 biobricks. In the front it has ptet(Bba_R0040) which is constitutively on. This produces LasR all the time with lasR(Bba_C0179) next in the sequence. Only in the presence of the Pseudomonas Autoinducer 1 (PA1), LasR forms an complex with PA1 and it can then activate the plasB(Bba_R0079) promoter that leads to the production of GFP(Bba_E0040).
mutable notes: All the inserts were digested with XbaI and PstI, and all the vectors were each time cut with SpeI and PstI.
mutable provenance: All the parts have come from existing iGEM distribution kits.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_C0179
component: BBa_R0079
component: BBa_R0040
component: BBa_E0040
sequence annotation: BBa_E0040
sequence annotation: BBa_R0079
sequence annotation: BBa_R0040
sequence annotation: BBa_C0179
sequence: https://synbiohub.org/public/igem/BBa_K1315009_sequence/1

BBa_C0179
type: Component
persistent identity: component2387055
display id: component2387055
version: 1
top level: BBa_K1315009
access: public
definition: https://synbiohub.org/public/igem/BBa_C0179/1

BBa_R0079
type: Component
persistent identity: component2387060
display id: component2387060
version: 1
top level: BBa_K1315009
access: public
definition: https://synbiohub.org/public/igem/BBa_R0079/1

BBa_R0040
type: Component
persistent identity: component2387050
display id: component2387050
version: 1
top level: BBa_K1315009
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_E0040
type: Component
persistent identity: component2387062
display id: component2387062
version: 1
top level: BBa_K1315009
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2387062
display id: annotation2387062
version: 1
top level: BBa_K1315009
location: https://synbiohub.org/public/igem/BBa_K1315009/annotation2387062/range2387062/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K1315009/annotation2387062/range2387062/1
type: Range
persistent identity: range2387062
display id: range2387062
version: 1
direction: direction/reverse
top level: BBa_K1315009
start: 955
end: 1674
orientation: inline

BBa_R0079
type: SequenceAnnotation
persistent identity: annotation2387060
display id: annotation2387060
version: 1
top level: BBa_K1315009
location: https://synbiohub.org/public/igem/BBa_K1315009/annotation2387060/range2387060/1
component: BBa_R0079

https://synbiohub.org/public/igem/BBa_K1315009/annotation2387060/range2387060/1
type: Range
persistent identity: range2387060
display id: range2387060
version: 1
direction: direction/reverse
top level: BBa_K1315009
start: 792
end: 948
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2387050
display id: annotation2387050
version: 1
top level: BBa_K1315009
location: https://synbiohub.org/public/igem/BBa_K1315009/annotation2387050/range2387050/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K1315009/annotation2387050/range2387050/1
type: Range
persistent identity: range2387050
display id: range2387050
version: 1
direction: direction/forward
top level: BBa_K1315009
start: 1
end: 54
orientation: inline

BBa_C0179
type: SequenceAnnotation
persistent identity: annotation2387055
display id: annotation2387055
version: 1
top level: BBa_K1315009
location: https://synbiohub.org/public/igem/BBa_K1315009/annotation2387055/range2387055/1
component: BBa_C0179

https://synbiohub.org/public/igem/BBa_K1315009/annotation2387055/range2387055/1
type: Range
persistent identity: range2387055
display id: range2387055
version: 1
direction: direction/reverse
top level: BBa_K1315009
start: 61
end: 783
orientation: inline

BBa_K1763435
type: ComponentDefinition
persistent identity: BBa K1763435
display id: BBa_K1763435
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: MaSp1-9(T7)
creator: Vinson Lam
created: 2015-09-14T11:00:00Z
modified: 2015-09-15T09:24:50Z
discontinued: false
dominant: false
group u list: _2185_
m user id: 27136
owner id: 27136
owning group id: 9
status: status/Planning
bookmark: false
mutable description: MaSp1 9 mer under control of T7 promoter and RBS
mutable notes: no.
mutable provenance: N. clavipes
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K525998
component: BBa_K1763434
sequence annotation: BBa_K1763434
sequence annotation: BBa_K525998
sequence: https://synbiohub.org/public/igem/BBa_K1763435_sequence/1

BBa_K525998
type: Component
persistent identity: component2456884
display id: component2456884
version: 1
top level: BBa_K1763435
access: public
definition: https://synbiohub.org/public/igem/BBa_K525998/1

BBa_K1763434
type: Component
persistent identity: component2456885
display id: component2456885
version: 1
top level: BBa_K1763435
access: public
definition: BBa_K1763434

BBa_K1763434
type: SequenceAnnotation
persistent identity: annotation2456885
display id: annotation2456885
version: 1
top level: BBa_K1763435
location: https://synbiohub.org/public/igem/BBa_K1763435/annotation2456885/range2456885/1
component: BBa_K1763434

https://synbiohub.org/public/igem/BBa_K1763435/annotation2456885/range2456885/1
type: Range
persistent identity: range2456885
display id: range2456885
version: 1
direction: direction/reverse
top level: BBa_K1763435
start: 39
end: 1007
orientation: inline

BBa_K525998
type: SequenceAnnotation
persistent identity: annotation2456884
display id: annotation2456884
version: 1
top level: BBa_K1763435
location: https://synbiohub.org/public/igem/BBa_K1763435/annotation2456884/range2456884/1
component: BBa_K525998

https://synbiohub.org/public/igem/BBa_K1763435/annotation2456884/range2456884/1
type: Range
persistent identity: range2456884
display id: range2456884
version: 1
direction: direction/reverse
top level: BBa_K1763435
start: 1
end: 32
orientation: inline

BBa_K1399005
type: ComponentDefinition
persistent identity: BBa K1399005
display id: BBa_K1399005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP (mut3b) with AAV-ssrA degradation tag
creator: Anna Stikane
created: 2014-09-18T11:00:00Z
modified: 2015-05-08T01:10:16Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1777_
m user id: 0
owner id: 22477
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: GFP (mut3b) (see part BBa_E0040) with added AAV-ssrA degradation tag (part BBa_I11012). The tag increases RFP turn-over rate, thus providing better temporal resolution of red fluorescence. In the same time, maximal fluorescence amplitudes will be lower as newly formed protein is degraded as soon as it is formed. The tag encodes peptide sequence AANDENYAAAV and is recognized by ClpA and ClpX unfoldases and ClpX mediator SspB.[1] ClpA and ClpX then form a proteosome-like complex with ClpP protease and the protein is degraded.[1] The final three residues of the tag determines the strength of interaction with ClpX and thus the final protein degradation rate.[2] The AAV tag is reported to lead to reasonably fast protein degradation, degrading GFP with rate -0.012 per minute.[2] However, be aware that exact protein degradation rate depends on multiple factors: ClpXP and ClpAP protease and SspB mediator concentrations, protein stability, Km of binding to the protease, temperature [3]. References: [1] Flynn, J. M. et al. Overlapping recognition determinants within the ssrA degradation tag allow modulation of proteolysis. Proc. Natl. Acad. Sci. U. S. A. 98, 10584???9 (2001). [2] Andersen, J. B. et al. New unstable variants of green fluorescent protein for studies of transient gene expression in bacteria. Appl. Environ. Microbiol. 64, 2240???6 (1998). [3] Purcell, O., Grierson, C. S., Bernardo, M. Di & Savery, N. J. Temperature dependence of ssrA-tag mediated protein degradation. J. Biol. Eng. 6, 10 (2012).
mutable notes: The tag was attached to RFP using PCR and MABEL (mutagenesis with blunt-end ligation), thus avoiding introduction of additonal residues and restriction site. Different parts of the tag are recognized by different proteins, for example, the final 3 residues (AAV in this case) are recognised by ClpX, whereas first 4 residues of the tag are required for efficient SspB binding.[1] Thus modifications of these critical residues alter the efficacy with what different proteases bind to it.
mutable provenance: GFP comes from part BBa_E0040, tag sequence was obtained from part I11012, but same tag was also used in paper by Andersen et al., (1998).[2]
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: GFP (mut3b)
sequence annotation: stop
sequence annotation: AAV-ssrA tag
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_K1399005_sequence/1

GFP (mut3b)
type: SequenceAnnotation
persistent identity: annotation2383909
display id: annotation2383909
version: 1
top level: BBa_K1399005
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1399005/annotation2383909/range2383909/1

https://synbiohub.org/public/igem/BBa_K1399005/annotation2383909/range2383909/1
type: Range
persistent identity: range2383909
display id: range2383909
version: 1
direction: direction/forward
top level: BBa_K1399005
start: 4
end: 714
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2383912
display id: annotation2383912
version: 1
top level: BBa_K1399005
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399005/annotation2383912/range2383912/1

https://synbiohub.org/public/igem/BBa_K1399005/annotation2383912/range2383912/1
type: Range
persistent identity: range2383912
display id: range2383912
version: 1
direction: direction/forward
top level: BBa_K1399005
start: 751
end: 753
orientation: inline

AAV-ssrA tag
type: SequenceAnnotation
persistent identity: annotation2383910
display id: annotation2383910
version: 1
top level: BBa_K1399005
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1399005/annotation2383910/range2383910/1

https://synbiohub.org/public/igem/BBa_K1399005/annotation2383910/range2383910/1
type: Range
persistent identity: range2383910
display id: range2383910
version: 1
direction: direction/forward
top level: BBa_K1399005
start: 715
end: 747
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2383908
display id: annotation2383908
version: 1
top level: BBa_K1399005
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1399005/annotation2383908/range2383908/1

https://synbiohub.org/public/igem/BBa_K1399005/annotation2383908/range2383908/1
type: Range
persistent identity: range2383908
display id: range2383908
version: 1
direction: direction/forward
top level: BBa_K1399005
start: 1
end: 3
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2383911
display id: annotation2383911
version: 1
top level: BBa_K1399005
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399005/annotation2383911/range2383911/1

https://synbiohub.org/public/igem/BBa_K1399005/annotation2383911/range2383911/1
type: Range
persistent identity: range2383911
display id: range2383911
version: 1
direction: direction/forward
top level: BBa_K1399005
start: 748
end: 750
orientation: inline

BBa_K1325005
type: ComponentDefinition
persistent identity: BBa K1325005
display id: BBa_K1325005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: MinC??????cell division inhibitor
creator: Ke Xu
created: 2014-09-28T11:00:00Z
modified: 2015-05-08T01:09:54Z
discontinued: false
dominant: false
group u list: _1700_
m user id: 0
owner id: 23253
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: cell division inhibitor of the MinC-MinD-MinE and DicB-MinC systems that regulate septum placement
mutable notes: no
mutable provenance: E.coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1325005_sequence/1

BBa_K1789006
type: ComponentDefinition
persistent identity: BBa K1789006
display id: BBa_K1789006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SCAF2
creator: Xinyuan Qiu
created: 2015-09-12T11:00:00Z
modified: 2015-09-18T08:33:02Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2214_
m user id: 21134
owner id: 26299
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This part is a highly repetitive DNA sequence which can bind TALE protein specifically.
mutable notes: This part is designed to contain two basic short sequences which can bind TALE1 and TALE2(3) specifically. These two basic sequences are repeated with no space by ten times, so it can bind more TALE protein and the spatial location of each pair of TALE1 and TALE2(3) is almost opposite.
mutable provenance: This part is synthetized by viewsolid biotech firm (Beijing, China).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: SCAF2
sequence annotation: BM2
sequence annotation: BM1
sequence: https://synbiohub.org/public/igem/BBa_K1789006_sequence/1

SCAF2
type: SequenceAnnotation
persistent identity: annotation2453186
display id: annotation2453186
version: 1
top level: BBa_K1789006
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1789006/annotation2453186/range2453186/1

https://synbiohub.org/public/igem/BBa_K1789006/annotation2453186/range2453186/1
type: Range
persistent identity: range2453186
display id: range2453186
version: 1
direction: direction/forward
top level: BBa_K1789006
start: 1
end: 462
orientation: inline

BM2
type: SequenceAnnotation
persistent identity: annotation2470918
display id: annotation2470918
version: 1
top level: BBa_K1789006
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1789006/annotation2470918/range2470918/1

https://synbiohub.org/public/igem/BBa_K1789006/annotation2470918/range2470918/1
type: Range
persistent identity: range2470918
display id: range2470918
version: 1
direction: direction/forward
top level: BBa_K1789006
start: 21
end: 34
orientation: inline

BM1
type: SequenceAnnotation
persistent identity: annotation2470907
display id: annotation2470907
version: 1
top level: BBa_K1789006
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1789006/annotation2470907/range2470907/1

https://synbiohub.org/public/igem/BBa_K1789006/annotation2470907/range2470907/1
type: Range
persistent identity: range2470907
display id: range2470907
version: 1
direction: direction/forward
top level: BBa_K1789006
start: 1
end: 14
orientation: inline

BBa_K1491005
type: ComponentDefinition
persistent identity: BBa K1491005
display id: BBa_K1491005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS-SuperYFP2-His
creator: Danielle Peters
created: 2014-07-14T11:00:00Z
modified: 2015-05-08T01:10:44Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1871_
m user id: 0
owner id: 22521
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: SuperYFP2 with 6x His tag(BBa_K1491004) and a strong RBS (BBa_B0034). It can be used as a reporter.
mutable notes: RBS+YFP+His
mutable provenance: RBS is BBa_B0034 and SuperYFP2 is BBa_K864100
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0034
component: BBa_K1491004
sequence annotation: BBa_K1491004
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K1491005_sequence/1

BBa_B0034
type: Component
persistent identity: component2380097
display id: component2380097
version: 1
top level: BBa_K1491005
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1491004
type: Component
persistent identity: component2380100
display id: component2380100
version: 1
top level: BBa_K1491005
access: public
definition: https://synbiohub.org/public/igem/BBa_K1491004/1

BBa_K1491004
type: SequenceAnnotation
persistent identity: annotation2380100
display id: annotation2380100
version: 1
top level: BBa_K1491005
location: https://synbiohub.org/public/igem/BBa_K1491005/annotation2380100/range2380100/1
component: BBa_K1491004

https://synbiohub.org/public/igem/BBa_K1491005/annotation2380100/range2380100/1
type: Range
persistent identity: range2380100
display id: range2380100
version: 1
direction: direction/reverse
top level: BBa_K1491005
start: 19
end: 759
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2380097
display id: annotation2380097
version: 1
top level: BBa_K1491005
location: https://synbiohub.org/public/igem/BBa_K1491005/annotation2380097/range2380097/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1491005/annotation2380097/range2380097/1
type: Range
persistent identity: range2380097
display id: range2380097
version: 1
direction: direction/reverse
top level: BBa_K1491005
start: 1
end: 12
orientation: inline

BBa_K1602010
type: ComponentDefinition
persistent identity: BBa K1602010
display id: BBa_K1602010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: xylC
creator: Leon Kraus, Jonathan Pletzer-Zelgert, Jonas Sindlinger, Carmen Sakas Gandullo, Sebastian Holzner, Elena Tonchevska, Mirjam Haun
created: 2015-09-13T11:00:00Z
modified: 2015-09-25T01:32:15Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2019_
m user id: 12512
owner id: 25878
owning group id: 9
status: status/Planning
bookmark: false
mutable description: BLANK
mutable notes: BLANK
mutable provenance: BLANK
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1602010_sequence/1

BBa_K1602013
type: ComponentDefinition
persistent identity: BBa K1602013
display id: BBa_K1602013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: yqhD
creator: Leon Kraus, Jonathan Pletzer-Zelgert, Jonas Sindlinger, Carmen Sakas Gandullo, Sebastian Holzner, Elena Tonchevska, Mirjam Haun
created: 2015-09-13T11:00:00Z
modified: 2015-09-18T06:39:43Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2019_
m user id: 26220
owner id: 25878
owning group id: 9
status: status/Planning
bookmark: false
mutable description: BLANK
mutable notes: BLANK
mutable provenance: BLANK
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1602013_sequence/1