iGEM 2019 Distribution Plate 2 Well 9E
type: Collection
persistent identity: iGEM 2019 Plate2 Well9E
display id: iGEM_2019_Plate2_Well9E
version: 1
owned by: myers
member: BBa_K823016

iGEM 2019 Distribution Plate 2 Well 23D
type: Collection
persistent identity: iGEM 2019 Plate2 Well23D
display id: iGEM_2019_Plate2_Well23D
version: 1
owned by: myers
member: GFP genera

iGEM 2019 Distribution Plate 2 Well 6C
type: Collection
persistent identity: iGEM 2019 Plate2 Well6C
display id: iGEM_2019_Plate2_Well6C
version: 1
owned by: myers
member: BBa_K876014

iGEM 2019 Distribution Plate 2 Well 5E
type: Collection
persistent identity: iGEM 2019 Plate2 Well5E
display id: iGEM_2019_Plate2_Well5E
version: 1
owned by: myers
member: BBa_K775003

iGEM 2019 Distribution Plate 2 Well 14O
type: Collection
persistent identity: iGEM 2019 Plate2 Well14O
display id: iGEM_2019_Plate2_Well14O
version: 1
owned by: myers
member: BBa_K909014

iGEM 2019 Distribution Plate 2 Well 5N
type: Collection
persistent identity: iGEM 2019 Plate2 Well5N
display id: iGEM_2019_Plate2_Well5N
version: 1
owned by: myers
member: lux pR

iGEM 2019 Distribution Plate 2 Well 3D
type: Collection
persistent identity: iGEM 2019 Plate2 Well3D
display id: iGEM_2019_Plate2_Well3D
version: 1
owned by: myers
member: cI 434

iGEM 2019 Distribution Plate 2 Well 6O
type: Collection
persistent identity: iGEM 2019 Plate2 Well6O
display id: iGEM_2019_Plate2_Well6O
version: 1
owned by: myers
member: BBa_K844000

iGEM 2019 Distribution Plate 2 Well 15J
type: Collection
persistent identity: iGEM 2019 Plate2 Well15J
display id: iGEM_2019_Plate2_Well15J
version: 1
owned by: myers
member: BBa_J37019

iGEM 2019 Distribution Plate 2 Well 8O
type: Collection
persistent identity: iGEM 2019 Plate2 Well8O
display id: iGEM_2019_Plate2_Well8O
version: 1
owned by: myers
member: PCaa3

iGEM 2019 Distribution Plate 2 Well 23P
type: Collection
persistent identity: iGEM 2019 Plate2 Well23P
display id: iGEM_2019_Plate2_Well23P
version: 1
owned by: myers
member: BBa_J04450

iGEM 2019 Distribution Plate 2 Well 11A
type: Collection
persistent identity: iGEM 2019 Plate2 Well11A
display id: iGEM_2019_Plate2_Well11A
version: 1
owned by: myers
member: BBa_S05055

iGEM 2019 Distribution Plate 2 Well 1I
type: Collection
persistent identity: iGEM 2019 Plate2 Well1I
display id: iGEM_2019_Plate2_Well1I
version: 1
owned by: myers
member: BBa_K567001

iGEM 2019 Distribution Plate 2 Well 11L
type: Collection
persistent identity: iGEM 2019 Plate2 Well11L
display id: iGEM_2019_Plate2_Well11L
version: 1
owned by: myers
member: BBa_I6066

iGEM 2019 Distribution Plate 2 Well 15I
type: Collection
persistent identity: iGEM 2019 Plate2 Well15I
display id: iGEM_2019_Plate2_Well15I
version: 1
owned by: myers
member: BBa_K861042

iGEM 2019 Distribution Plate 2 Well 16B
type: Collection
persistent identity: iGEM 2019 Plate2 Well16B
display id: iGEM_2019_Plate2_Well16B
version: 1
owned by: myers
member: BBa_S03855

iGEM 2019 Distribution Plate 2 Well 12G
type: Collection
persistent identity: iGEM 2019 Plate2 Well12G
display id: iGEM_2019_Plate2_Well12G
version: 1
owned by: myers
member: BBa_J202004

iGEM 2019 Distribution Plate 2 Well 1M
type: Collection
persistent identity: iGEM 2019 Plate2 Well1M
display id: iGEM_2019_Plate2_Well1M
version: 1
owned by: myers
member: BBa_K567015

iGEM 2019 Distribution Plate 2 Well 6E
type: Collection
persistent identity: iGEM 2019 Plate2 Well6E
display id: iGEM_2019_Plate2_Well6E
version: 1
owned by: myers
member: BBa_K876015

iGEM 2019 Distribution Plate 2 Well 1K
type: Collection
persistent identity: iGEM 2019 Plate2 Well1K
display id: iGEM_2019_Plate2_Well1K
version: 1
owned by: myers
member: BBa_K567008

iGEM 2019 Distribution Plate 2 Well 12E
type: Collection
persistent identity: iGEM 2019 Plate2 Well12E
display id: iGEM_2019_Plate2_Well12E
version: 1
owned by: myers
member: BBa_J202003

iGEM 2019 Distribution Plate 2 Well 12H
type: Collection
persistent identity: iGEM 2019 Plate2 Well12H
display id: iGEM_2019_Plate2_Well12H
version: 1
owned by: myers
member: BBa_I714036

iGEM 2019 Distribution Plate 2 Well 5I
type: Collection
persistent identity: iGEM 2019 Plate2 Well5I
display id: iGEM_2019_Plate2_Well5I
version: 1
owned by: myers
member: araC-CysE

iGEM 2019 Distribution Plate 2 Well 11B
type: Collection
persistent identity: iGEM 2019 Plate2 Well11B
display id: iGEM_2019_Plate2_Well11B
version: 1
owned by: myers
member: BBa_I6046

iGEM 2019 Distribution Plate 2 Well 24H
type: Collection
persistent identity: iGEM 2019 Plate2 Well24H
display id: iGEM_2019_Plate2_Well24H
version: 1
owned by: myers
member: BBa_I746104

iGEM 2019 Distribution Plate 2 Well 6P
type: Collection
persistent identity: iGEM 2019 Plate2 Well6P
display id: iGEM_2019_Plate2_Well6P
version: 1
owned by: myers
member: BBa_I13202

iGEM 2019 Distribution Plate 2 Well 20K
type: Collection
persistent identity: iGEM 2019 Plate2 Well20K
display id: iGEM_2019_Plate2_Well20K
version: 1
owned by: myers
member: BBa_K876038

iGEM 2019 Distribution Plate 2 Well 23O
type: Collection
persistent identity: iGEM 2019 Plate2 Well23O
display id: iGEM_2019_Plate2_Well23O
version: 1
owned by: myers
member: BBa_J04450

iGEM 2019 Distribution Plate 2 Well 17O
type: Collection
persistent identity: iGEM 2019 Plate2 Well17O
display id: iGEM_2019_Plate2_Well17O
version: 1
owned by: myers
member: BBa_K741008

iGEM 2019 Distribution Plate 2 Well 5C
type: Collection
persistent identity: iGEM 2019 Plate2 Well5C
display id: iGEM_2019_Plate2_Well5C
version: 1
owned by: myers
member: BBa_K775002

iGEM 2019 Distribution Plate 2 Well 15F
type: Collection
persistent identity: iGEM 2019 Plate2 Well15F
display id: iGEM_2019_Plate2_Well15F
version: 1
owned by: myers
member: BBa_I13013

iGEM 2019 Distribution Plate 2 Well 22M
type: Collection
persistent identity: iGEM 2019 Plate2 Well22M
display id: iGEM_2019_Plate2_Well22M
version: 1
owned by: myers
member: BBa_K952003

iGEM 2019 Distribution Plate 2 Well 11M
type: Collection
persistent identity: iGEM 2019 Plate2 Well11M
display id: iGEM_2019_Plate2_Well11M
version: 1
owned by: myers
member: TD1.0

iGEM 2019 Distribution Plate 2 Well 13I
type: Collection
persistent identity: iGEM 2019 Plate2 Well13I
display id: iGEM_2019_Plate2_Well13I
version: 1
owned by: myers
member: BBa_K863000

iGEM 2019 Distribution Plate 2 Well 11I
type: Collection
persistent identity: iGEM 2019 Plate2 Well11I
display id: iGEM_2019_Plate2_Well11I
version: 1
owned by: myers
member: BBa_K733018

iGEM 2019 Distribution Plate 2 Well 4K
type: Collection
persistent identity: iGEM 2019 Plate2 Well4K
display id: iGEM_2019_Plate2_Well4K
version: 1
owned by: myers
member: BBa_K784006

iGEM 2019 Distribution Plate 2 Well 10N
type: Collection
persistent identity: iGEM 2019 Plate2 Well10N
display id: iGEM_2019_Plate2_Well10N
version: 1
owned by: myers
member: BBa_I746324

iGEM 2019 Distribution Plate 2 Well 10F
type: Collection
persistent identity: iGEM 2019 Plate2 Well10F
display id: iGEM_2019_Plate2_Well10F
version: 1
owned by: myers
member: BBa_I732081

iGEM 2019 Distribution Plate 2 Well 8J
type: Collection
persistent identity: iGEM 2019 Plate2 Well8J
display id: iGEM_2019_Plate2_Well8J
version: 1
owned by: myers
member: BBa_I732904

iGEM 2019 Distribution Plate 2 Well 20F
type: Collection
persistent identity: iGEM 2019 Plate2 Well20F
display id: iGEM_2019_Plate2_Well20F
version: 1
owned by: myers
member: BBa_S03154

iGEM 2019 Distribution Plate 2 Well 5G
type: Collection
persistent identity: iGEM 2019 Plate2 Well5G
display id: iGEM_2019_Plate2_Well5G
version: 1
owned by: myers
member: otsA

iGEM 2019 Distribution Plate 2 Well 16A
type: Collection
persistent identity: iGEM 2019 Plate2 Well16A
display id: iGEM_2019_Plate2_Well16A
version: 1
owned by: myers
member: BBa_K909015

iGEM 2019 Distribution Plate 2 Well 13B
type: Collection
persistent identity: iGEM 2019 Plate2 Well13B
display id: iGEM_2019_Plate2_Well13B
version: 1
owned by: myers
member: BBa_I6080

iGEM 2019 Distribution Plate 2 Well 18C
type: Collection
persistent identity: iGEM 2019 Plate2 Well18C
display id: iGEM_2019_Plate2_Well18C
version: 1
owned by: myers
member: BBa_K880002

iGEM 2019 Distribution Plate 2 Well 4M
type: Collection
persistent identity: iGEM 2019 Plate2 Well4M
display id: iGEM_2019_Plate2_Well4M
version: 1
owned by: myers
member: BBa_K784013

iGEM 2019 Distribution Plate 2 Well 23G
type: Collection
persistent identity: iGEM 2019 Plate2 Well23G
display id: iGEM_2019_Plate2_Well23G
version: 1
owned by: myers
member: Cel5A

iGEM 2019 Distribution Plate 2 Well 12N
type: Collection
persistent identity: iGEM 2019 Plate2 Well12N
display id: iGEM_2019_Plate2_Well12N
version: 1
owned by: myers
member: BBa_I716210

iGEM 2019 Distribution Plate 2 Well 6K
type: Collection
persistent identity: iGEM 2019 Plate2 Well6K
display id: iGEM_2019_Plate2_Well6K
version: 1
owned by: myers
member: BBa_K876070

iGEM 2019 Distribution Plate 2 Well 8D
type: Collection
persistent identity: iGEM 2019 Plate2 Well8D
display id: iGEM_2019_Plate2_Well8D
version: 1
owned by: myers
member: BBa_I13602

iGEM 2019 Distribution Plate 2 Well 5D
type: Collection
persistent identity: iGEM 2019 Plate2 Well5D
display id: iGEM_2019_Plate2_Well5D
version: 1
owned by: myers
member: lacI+pL

iGEM 2019 Distribution Plate 2 Well 4J
type: Collection
persistent identity: iGEM 2019 Plate2 Well4J
display id: iGEM_2019_Plate2_Well4J
version: 1
owned by: myers
member: OmpR

iGEM 2019 Distribution Plate 2 Well 23L
type: Collection
persistent identity: iGEM 2019 Plate2 Well23L
display id: iGEM_2019_Plate2_Well23L
version: 1
owned by: myers
member: BBa_I9026

iGEM 2019 Distribution Plate 2 Well 20D
type: Collection
persistent identity: iGEM 2019 Plate2 Well20D
display id: iGEM_2019_Plate2_Well20D
version: 1
owned by: myers
member: BBa_I13600

iGEM 2019 Distribution Plate 2 Well 17M
type: Collection
persistent identity: iGEM 2019 Plate2 Well17M
display id: iGEM_2019_Plate2_Well17M
version: 1
owned by: myers
member: BBa_K741002

iGEM 2019 Distribution Plate 2 Well 10I
type: Collection
persistent identity: iGEM 2019 Plate2 Well10I
display id: iGEM_2019_Plate2_Well10I
version: 1
owned by: myers
member: BBa_K916000

iGEM 2019 Distribution Plate 2 Well 20A
type: Collection
persistent identity: iGEM 2019 Plate2 Well20A
display id: iGEM_2019_Plate2_Well20A
version: 1
owned by: myers
member: BBa_K784047

iGEM 2019 Distribution Plate 2 Well 12K
type: Collection
persistent identity: iGEM 2019 Plate2 Well12K
display id: iGEM_2019_Plate2_Well12K
version: 1
owned by: myers
member: BBa_J202006

iGEM 2019 Distribution Plate 2 Well 18D
type: Collection
persistent identity: iGEM 2019 Plate2 Well18D
display id: iGEM_2019_Plate2_Well18D
version: 1
owned by: myers
member: BBa_I13504

iGEM 2019 Distribution Plate 2 Well 12P
type: Collection
persistent identity: iGEM 2019 Plate2 Well12P
display id: iGEM_2019_Plate2_Well12P
version: 1
owned by: myers
member: BBa_I716211

iGEM 2019 Distribution Plate 2 Well 4O
type: Collection
persistent identity: iGEM 2019 Plate2 Well4O
display id: iGEM_2019_Plate2_Well4O
version: 1
owned by: myers
member: BBa_K784014

iGEM 2019 Distribution Plate 2 Well 20L
type: Collection
persistent identity: iGEM 2019 Plate2 Well20L
display id: iGEM_2019_Plate2_Well20L
version: 1
owned by: myers
member: BBa_E0430

iGEM 2019 Distribution Plate 2 Well 1L
type: Collection
persistent identity: iGEM 2019 Plate2 Well1L
display id: iGEM_2019_Plate2_Well1L
version: 1
owned by: myers
member: BBa_B0033

iGEM 2019 Distribution Plate 2 Well 6N
type: Collection
persistent identity: iGEM 2019 Plate2 Well6N
display id: iGEM_2019_Plate2_Well6N
version: 1
owned by: myers
member: BBa_I13028

iGEM 2019 Distribution Plate 2 Well 2J
type: Collection
persistent identity: iGEM 2019 Plate2 Well2J
display id: iGEM_2019_Plate2_Well2J
version: 1
owned by: myers
member: EYFP

iGEM 2019 Distribution Plate 2 Well 2G
type: Collection
persistent identity: iGEM 2019 Plate2 Well2G
display id: iGEM_2019_Plate2_Well2G
version: 1
owned by: myers
member: BBa_K812032

iGEM 2019 Distribution Plate 2 Well 2M
type: Collection
persistent identity: iGEM 2019 Plate2 Well2M
display id: iGEM_2019_Plate2_Well2M
version: 1
owned by: myers
member: BBa_K876000

iGEM 2019 Distribution Plate 2 Well 3F
type: Collection
persistent identity: iGEM 2019 Plate2 Well3F
display id: iGEM_2019_Plate2_Well3F
version: 1
owned by: myers
member: c2 P22

iGEM 2019 Distribution Plate 2 Well 12J
type: Collection
persistent identity: iGEM 2019 Plate2 Well12J
display id: iGEM_2019_Plate2_Well12J
version: 1
owned by: myers
member: RBS-LacY

iGEM 2019 Distribution Plate 2 Well 23H
type: Collection
persistent identity: iGEM 2019 Plate2 Well23H
display id: iGEM_2019_Plate2_Well23H
version: 1
owned by: myers
member: BBa_S01002

iGEM 2019 Distribution Plate 2 Well 16P
type: Collection
persistent identity: iGEM 2019 Plate2 Well16P
display id: iGEM_2019_Plate2_Well16P
version: 1
owned by: myers
member: BBa_I13500

iGEM 2019 Distribution Plate 2 Well 8B
type: Collection
persistent identity: iGEM 2019 Plate2 Well8B
display id: iGEM_2019_Plate2_Well8B
version: 1
owned by: myers
member: BBa_A340620

iGEM 2019 Distribution Plate 2 Well 21B
type: Collection
persistent identity: iGEM 2019 Plate2 Well21B
display id: iGEM_2019_Plate2_Well21B
version: 1
owned by: myers
member: BBa_I732903

iGEM 2019 Distribution Plate 2 Well 1D
type: Collection
persistent identity: iGEM 2019 Plate2 Well1D
display id: iGEM_2019_Plate2_Well1D
version: 1
owned by: myers
member: BBa_B0013

iGEM 2019 Distribution Plate 2 Well 1E
type: Collection
persistent identity: iGEM 2019 Plate2 Well1E
display id: iGEM_2019_Plate2_Well1E
version: 1
owned by: myers
member: BBa_K598019

iGEM 2019 Distribution Plate 2 Well 7G
type: Collection
persistent identity: iGEM 2019 Plate2 Well7G
display id: iGEM_2019_Plate2_Well7G
version: 1
owned by: myers
member: tctA_503aa

iGEM 2019 Distribution Plate 2 Well 20I
type: Collection
persistent identity: iGEM 2019 Plate2 Well20I
display id: iGEM_2019_Plate2_Well20I
version: 1
owned by: myers
member: BBa_K876013

iGEM 2019 Distribution Plate 2 Well 3P
type: Collection
persistent identity: iGEM 2019 Plate2 Well3P
display id: iGEM_2019_Plate2_Well3P
version: 1
owned by: myers
member: ECFP

iGEM 2019 Distribution Plate 2 Well 22A
type: Collection
persistent identity: iGEM 2019 Plate2 Well22A
display id: iGEM_2019_Plate2_Well22A
version: 1
owned by: myers
member: BBa_K876063

iGEM 2019 Distribution Plate 2 Well 3H
type: Collection
persistent identity: iGEM 2019 Plate2 Well3H
display id: iGEM_2019_Plate2_Well3H
version: 1
owned by: myers
member: aiiA

iGEM 2019 Distribution Plate 2 Well 12I
type: Collection
persistent identity: iGEM 2019 Plate2 Well12I
display id: iGEM_2019_Plate2_Well12I
version: 1
owned by: myers
member: BBa_J202005

iGEM 2019 Distribution Plate 2 Well 21H
type: Collection
persistent identity: iGEM 2019 Plate2 Well21H
display id: iGEM_2019_Plate2_Well21H
version: 1
owned by: myers
member: BBa_I6106

iGEM 2019 Distribution Plate 2 Well 14H
type: Collection
persistent identity: iGEM 2019 Plate2 Well14H
display id: iGEM_2019_Plate2_Well14H
version: 1
owned by: myers
member: BBa_I718011

iGEM 2019 Distribution Plate 2 Well 9N
type: Collection
persistent identity: iGEM 2019 Plate2 Well9N
display id: iGEM_2019_Plate2_Well9N
version: 1
owned by: myers
member: BBa_I6042

iGEM 2019 Distribution Plate 2 Well 6L
type: Collection
persistent identity: iGEM 2019 Plate2 Well6L
display id: iGEM_2019_Plate2_Well6L
version: 1
owned by: myers
member: BBa_I13027

iGEM 2019 Distribution Plate 2 Well 1J
type: Collection
persistent identity: iGEM 2019 Plate2 Well1J
display id: iGEM_2019_Plate2_Well1J
version: 1
owned by: myers
member: BBa_B0032

iGEM 2019 Distribution Plate 2 Well 22I
type: Collection
persistent identity: iGEM 2019 Plate2 Well22I
display id: iGEM_2019_Plate2_Well22I
version: 1
owned by: myers
member: BBa_K737005

iGEM 2019 Distribution Plate 2 Well 13E
type: Collection
persistent identity: iGEM 2019 Plate2 Well13E
display id: iGEM_2019_Plate2_Well13E
version: 1
owned by: myers
member: BBa_K769020

iGEM 2019 Distribution Plate 2 Well 13F
type: Collection
persistent identity: iGEM 2019 Plate2 Well13F
display id: iGEM_2019_Plate2_Well13F
version: 1
owned by: myers
member: BBa_I6086

iGEM 2019 Distribution Plate 2 Well 14J
type: Collection
persistent identity: iGEM 2019 Plate2 Well14J
display id: iGEM_2019_Plate2_Well14J
version: 1
owned by: myers
member: BBa_I718012

iGEM 2019 Distribution Plate 2 Well 24F
type: Collection
persistent identity: iGEM 2019 Plate2 Well24F
display id: iGEM_2019_Plate2_Well24F
version: 1
owned by: myers
member: RFP1

iGEM 2019 Distribution Plate 2 Well 10D
type: Collection
persistent identity: iGEM 2019 Plate2 Well10D
display id: iGEM_2019_Plate2_Well10D
version: 1
owned by: myers
member: BBa_I732097

iGEM 2019 Distribution Plate 2 Well 4P
type: Collection
persistent identity: iGEM 2019 Plate2 Well4P
display id: iGEM_2019_Plate2_Well4P
version: 1
owned by: myers
member: PenI

iGEM 2019 Distribution Plate 2 Well 11D
type: Collection
persistent identity: iGEM 2019 Plate2 Well11D
display id: iGEM_2019_Plate2_Well11D
version: 1
owned by: myers
member: BBa_I6050

iGEM 2019 Distribution Plate 2 Well 11H
type: Collection
persistent identity: iGEM 2019 Plate2 Well11H
display id: iGEM_2019_Plate2_Well11H
version: 1
owned by: myers
member: BBa_I6060

iGEM 2019 Distribution Plate 2 Well 18P
type: Collection
persistent identity: iGEM 2019 Plate2 Well18P
display id: iGEM_2019_Plate2_Well18P
version: 1
owned by: myers
member: BBa_J23119

iGEM 2019 Distribution Plate 2 Well 15G
type: Collection
persistent identity: iGEM 2019 Plate2 Well15G
display id: iGEM_2019_Plate2_Well15G
version: 1
owned by: myers
member: BBa_K861031

iGEM 2019 Distribution Plate 2 Well 19I
type: Collection
persistent identity: iGEM 2019 Plate2 Well19I
display id: iGEM_2019_Plate2_Well19I
version: 1
owned by: myers
member: fhlA

iGEM 2019 Distribution Plate 2 Well 12O
type: Collection
persistent identity: iGEM 2019 Plate2 Well12O
display id: iGEM_2019_Plate2_Well12O
version: 1
owned by: myers
member: BBa_J202008

iGEM 2019 Distribution Plate 2 Well 19G
type: Collection
persistent identity: iGEM 2019 Plate2 Well19G
display id: iGEM_2019_Plate2_Well19G
version: 1
owned by: myers
member: BBa_K929003

iGEM 2019 Distribution Plate 2 Well 4G
type: Collection
persistent identity: iGEM 2019 Plate2 Well4G
display id: iGEM_2019_Plate2_Well4G
version: 1
owned by: myers
member: BBa_K801010

iGEM 2019 Distribution Plate 2 Well 5J
type: Collection
persistent identity: iGEM 2019 Plate2 Well5J
display id: iGEM_2019_Plate2_Well5J
version: 1
owned by: myers
member: lux pL

iGEM 2019 Distribution Plate 2 Well 6D
type: Collection
persistent identity: iGEM 2019 Plate2 Well6D
display id: iGEM_2019_Plate2_Well6D
version: 1
owned by: myers
member: LasR+PAI

iGEM 2019 Distribution Plate 2 Well 10G
type: Collection
persistent identity: iGEM 2019 Plate2 Well10G
display id: iGEM_2019_Plate2_Well10G
version: 1
owned by: myers
member: ArgBox16

iGEM 2019 Distribution Plate 2 Well 15D
type: Collection
persistent identity: iGEM 2019 Plate2 Well15D
display id: iGEM_2019_Plate2_Well15D
version: 1
owned by: myers
member: BBa_I14044

iGEM 2019 Distribution Plate 2 Well 16C
type: Collection
persistent identity: iGEM 2019 Plate2 Well16C
display id: iGEM_2019_Plate2_Well16C
version: 1
owned by: myers
member: BBa_K909005

iGEM 2019 Distribution Plate 2 Well 18H
type: Collection
persistent identity: iGEM 2019 Plate2 Well18H
display id: iGEM_2019_Plate2_Well18H
version: 1
owned by: myers
member: ecfp

iGEM 2019 Distribution Plate 2 Well 13A
type: Collection
persistent identity: iGEM 2019 Plate2 Well13A
display id: iGEM_2019_Plate2_Well13A
version: 1
owned by: myers
member: TD0.01

iGEM 2019 Distribution Plate 2 Well 22L
type: Collection
persistent identity: iGEM 2019 Plate2 Well22L
display id: iGEM_2019_Plate2_Well22L
version: 1
owned by: myers
member: BBa_J09855

iGEM 2019 Distribution Plate 2 Well 16G
type: Collection
persistent identity: iGEM 2019 Plate2 Well16G
display id: iGEM_2019_Plate2_Well16G
version: 1
owned by: myers
member: BBa_K812020

iGEM 2019 Distribution Plate 2 Well 17C
type: Collection
persistent identity: iGEM 2019 Plate2 Well17C
display id: iGEM_2019_Plate2_Well17C
version: 1
owned by: myers
member: BBa_K786002

iGEM 2019 Distribution Plate 2 Well 19N
type: Collection
persistent identity: iGEM 2019 Plate2 Well19N
display id: iGEM_2019_Plate2_Well19N
version: 1
owned by: myers
member: BBa_I732902

iGEM 2019 Distribution Plate 2 Well 11O
type: Collection
persistent identity: iGEM 2019 Plate2 Well11O
display id: iGEM_2019_Plate2_Well11O
version: 1
owned by: myers
member: TD0.6

iGEM 2019 Distribution Plate 2 Well 2N
type: Collection
persistent identity: iGEM 2019 Plate2 Well2N
display id: iGEM_2019_Plate2_Well2N
version: 1
owned by: myers
member: BBa_E5501

iGEM 2019 Distribution Plate 2 Well 22J
type: Collection
persistent identity: iGEM 2019 Plate2 Well22J
display id: iGEM_2019_Plate2_Well22J
version: 1
owned by: myers
member: BBa_J24679

iGEM 2019 Distribution Plate 2 Well 19F
type: Collection
persistent identity: iGEM 2019 Plate2 Well19F
display id: iGEM_2019_Plate2_Well19F
version: 1
owned by: myers
member: [pTET][loc

iGEM 2019 Distribution Plate 2 Well 19K
type: Collection
persistent identity: iGEM 2019 Plate2 Well19K
display id: iGEM_2019_Plate2_Well19K
version: 1
owned by: myers
member: fhlA-E363

iGEM 2019 Distribution Plate 2 Well 15C
type: Collection
persistent identity: iGEM 2019 Plate2 Well15C
display id: iGEM_2019_Plate2_Well15C
version: 1
owned by: myers
member: BBa_K863011

iGEM 2019 Distribution Plate 2 Well 7J
type: Collection
persistent identity: iGEM 2019 Plate2 Well7J
display id: iGEM_2019_Plate2_Well7J
version: 1
owned by: myers
member: BBa_S0110

iGEM 2019 Distribution Plate 2 Well 2L
type: Collection
persistent identity: iGEM 2019 Plate2 Well2L
display id: iGEM_2019_Plate2_Well2L
version: 1
owned by: myers
member: aiiA

iGEM 2019 Distribution Plate 2 Well 14F
type: Collection
persistent identity: iGEM 2019 Plate2 Well14F
display id: iGEM_2019_Plate2_Well14F
version: 1
owned by: myers
member: BBa_I718009

iGEM 2019 Distribution Plate 2 Well 21E
type: Collection
persistent identity: iGEM 2019 Plate2 Well21E
display id: iGEM_2019_Plate2_Well21E
version: 1
owned by: myers
member: BBa_K849003

iGEM 2019 Distribution Plate 2 Well 6I
type: Collection
persistent identity: iGEM 2019 Plate2 Well6I
display id: iGEM_2019_Plate2_Well6I
version: 1
owned by: myers
member: BBa_K876069

iGEM 2019 Distribution Plate 2 Well 10J
type: Collection
persistent identity: iGEM 2019 Plate2 Well10J
display id: iGEM_2019_Plate2_Well10J
version: 1
owned by: myers
member: BBa_I715039

iGEM 2019 Distribution Plate 2 Well 20G
type: Collection
persistent identity: iGEM 2019 Plate2 Well20G
display id: iGEM_2019_Plate2_Well20G
version: 1
owned by: myers
member: BBa_K876011

iGEM 2019 Distribution Plate 2 Well 4N
type: Collection
persistent identity: iGEM 2019 Plate2 Well4N
display id: iGEM_2019_Plate2_Well4N
version: 1
owned by: myers
member: cinR

iGEM 2019 Distribution Plate 2 Well 15O
type: Collection
persistent identity: iGEM 2019 Plate2 Well15O
display id: iGEM_2019_Plate2_Well15O
version: 1
owned by: myers
member: BBa_K797000

iGEM 2019 Distribution Plate 2 Well 22D
type: Collection
persistent identity: iGEM 2019 Plate2 Well22D
display id: iGEM_2019_Plate2_Well22D
version: 1
owned by: myers
member: BBa_J44001

iGEM 2019 Distribution Plate 2 Well 9O
type: Collection
persistent identity: iGEM 2019 Plate2 Well9O
display id: iGEM_2019_Plate2_Well9O
version: 1
owned by: myers
member: LacIM

iGEM 2019 Distribution Plate 2 Well 3K
type: Collection
persistent identity: iGEM 2019 Plate2 Well3K
display id: iGEM_2019_Plate2_Well3K
version: 1
owned by: myers
member: BBa_K561000

iGEM 2019 Distribution Plate 2 Well 7I
type: Collection
persistent identity: iGEM 2019 Plate2 Well7I
display id: iGEM_2019_Plate2_Well7I
version: 1
owned by: myers
member: tctA_505aa

iGEM 2019 Distribution Plate 2 Well 14B
type: Collection
persistent identity: iGEM 2019 Plate2 Well14B
display id: iGEM_2019_Plate2_Well14B
version: 1
owned by: myers
member: PT7-D

iGEM 2019 Distribution Plate 2 Well 6B
type: Collection
persistent identity: iGEM 2019 Plate2 Well6B
display id: iGEM_2019_Plate2_Well6B
version: 1
owned by: myers
member: BBa_S03119

iGEM 2019 Distribution Plate 2 Well 10P
type: Collection
persistent identity: iGEM 2019 Plate2 Well10P
display id: iGEM_2019_Plate2_Well10P
version: 1
owned by: myers
member: BBa_I746105

iGEM 2019 Distribution Plate 2 Well 18F
type: Collection
persistent identity: iGEM 2019 Plate2 Well18F
display id: iGEM_2019_Plate2_Well18F
version: 1
owned by: myers
member: ecfp

iGEM 2019 Distribution Plate 2 Well 5B
type: Collection
persistent identity: iGEM 2019 Plate2 Well5B
display id: iGEM_2019_Plate2_Well5B
version: 1
owned by: myers
member: YFP

iGEM 2019 Distribution Plate 2 Well 14N
type: Collection
persistent identity: iGEM 2019 Plate2 Well14N
display id: iGEM_2019_Plate2_Well14N
version: 1
owned by: myers
member: BBa_I718014

iGEM 2019 Distribution Plate 2 Well 15A
type: Collection
persistent identity: iGEM 2019 Plate2 Well15A
display id: iGEM_2019_Plate2_Well15A
version: 1
owned by: myers
member: BBa_K863010

iGEM 2019 Distribution Plate 2 Well 19P
type: Collection
persistent identity: iGEM 2019 Plate2 Well19P
display id: iGEM_2019_Plate2_Well19P
version: 1
owned by: myers
member: BBa_I732805

iGEM 2019 Distribution Plate 2 Well 9H
type: Collection
persistent identity: iGEM 2019 Plate2 Well9H
display id: iGEM_2019_Plate2_Well9H
version: 1
owned by: myers
member: BBa_I6031

iGEM 2019 Distribution Plate 2 Well 16M
type: Collection
persistent identity: iGEM 2019 Plate2 Well16M
display id: iGEM_2019_Plate2_Well16M
version: 1
owned by: myers
member: BBa_K876004

iGEM 2019 Distribution Plate 2 Well 23C
type: Collection
persistent identity: iGEM 2019 Plate2 Well23C
display id: iGEM_2019_Plate2_Well23C
version: 1
owned by: myers
member: BBa_K953002

iGEM 2019 Distribution Plate 2 Well 6M
type: Collection
persistent identity: iGEM 2019 Plate2 Well6M
display id: iGEM_2019_Plate2_Well6M
version: 1
owned by: myers
member: BBa_K817000

iGEM 2019 Distribution Plate 2 Well 5A
type: Collection
persistent identity: iGEM 2019 Plate2 Well5A
display id: iGEM_2019_Plate2_Well5A
version: 1
owned by: myers
member: BBa_K775001

iGEM 2019 Distribution Plate 2 Well 16O
type: Collection
persistent identity: iGEM 2019 Plate2 Well16O
display id: iGEM_2019_Plate2_Well16O
version: 1
owned by: myers
member: BBa_K909003

iGEM 2019 Distribution Plate 2 Well 15N
type: Collection
persistent identity: iGEM 2019 Plate2 Well15N
display id: iGEM_2019_Plate2_Well15N
version: 1
owned by: myers
member: BBa_J61051

iGEM 2019 Distribution Plate 2 Well 22N
type: Collection
persistent identity: iGEM 2019 Plate2 Well22N
display id: iGEM_2019_Plate2_Well22N
version: 1
owned by: myers
member: BBa_I13458

iGEM 2019 Distribution Plate 2 Well 17B
type: Collection
persistent identity: iGEM 2019 Plate2 Well17B
display id: iGEM_2019_Plate2_Well17B
version: 1
owned by: myers
member: BBa_J13023

iGEM 2019 Distribution Plate 2 Well 20M
type: Collection
persistent identity: iGEM 2019 Plate2 Well20M
display id: iGEM_2019_Plate2_Well20M
version: 1
owned by: myers
member: BBa_K876052

iGEM 2019 Distribution Plate 2 Well 14I
type: Collection
persistent identity: iGEM 2019 Plate2 Well14I
display id: iGEM_2019_Plate2_Well14I
version: 1
owned by: myers
member: BBa_J202013

iGEM 2019 Distribution Plate 2 Well 8H
type: Collection
persistent identity: iGEM 2019 Plate2 Well8H
display id: iGEM_2019_Plate2_Well8H
version: 1
owned by: myers
member: BBa_I732815

iGEM 2019 Distribution Plate 2 Well 20H
type: Collection
persistent identity: iGEM 2019 Plate2 Well20H
display id: iGEM_2019_Plate2_Well20H
version: 1
owned by: myers
member: BBa_I0466

iGEM 2019 Distribution Plate 2 Well 14K
type: Collection
persistent identity: iGEM 2019 Plate2 Well14K
display id: iGEM_2019_Plate2_Well14K
version: 1
owned by: myers
member: BBa_J202014

iGEM 2019 Distribution Plate 2 Well 12M
type: Collection
persistent identity: iGEM 2019 Plate2 Well12M
display id: iGEM_2019_Plate2_Well12M
version: 1
owned by: myers
member: BBa_J202007

iGEM 2019 Distribution Plate 2 Well 23E
type: Collection
persistent identity: iGEM 2019 Plate2 Well23E
display id: iGEM_2019_Plate2_Well23E
version: 1
owned by: myers
member: RO

iGEM 2019 Distribution Plate 2 Well 2H
type: Collection
persistent identity: iGEM 2019 Plate2 Well2H
display id: iGEM_2019_Plate2_Well2H
version: 1
owned by: myers
member: ECFP

iGEM 2019 Distribution Plate 2 Well 9P
type: Collection
persistent identity: iGEM 2019 Plate2 Well9P
display id: iGEM_2019_Plate2_Well9P
version: 1
owned by: myers
member: BBa_I6044

iGEM 2019 Distribution Plate 2 Well 16J
type: Collection
persistent identity: iGEM 2019 Plate2 Well16J
display id: iGEM_2019_Plate2_Well16J
version: 1
owned by: myers
member: rbs+COMT

iGEM 2019 Distribution Plate 2 Well 14L
type: Collection
persistent identity: iGEM 2019 Plate2 Well14L
display id: iGEM_2019_Plate2_Well14L
version: 1
owned by: myers
member: BBa_I718013

iGEM 2019 Distribution Plate 2 Well 4L
type: Collection
persistent identity: iGEM 2019 Plate2 Well4L
display id: iGEM_2019_Plate2_Well4L
version: 1
owned by: myers
member: OmpR

iGEM 2019 Distribution Plate 2 Well 4E
type: Collection
persistent identity: iGEM 2019 Plate2 Well4E
display id: iGEM_2019_Plate2_Well4E
version: 1
owned by: myers
member: BBa_K880005

iGEM 2019 Distribution Plate 2 Well 5M
type: Collection
persistent identity: iGEM 2019 Plate2 Well5M
display id: iGEM_2019_Plate2_Well5M
version: 1
owned by: myers
member: BBa_K731250

iGEM 2019 Distribution Plate 2 Well 23K
type: Collection
persistent identity: iGEM 2019 Plate2 Well23K
display id: iGEM_2019_Plate2_Well23K
version: 1
owned by: myers
member: BBa_K909008

iGEM 2019 Distribution Plate 2 Well 3A
type: Collection
persistent identity: iGEM 2019 Plate2 Well3A
display id: iGEM_2019_Plate2_Well3A
version: 1
owned by: myers
member: BBa_K649303

iGEM 2019 Distribution Plate 2 Well 4C
type: Collection
persistent identity: iGEM 2019 Plate2 Well4C
display id: iGEM_2019_Plate2_Well4C
version: 1
owned by: myers
member: BBa_K773004

iGEM 2019 Distribution Plate 2 Well 15P
type: Collection
persistent identity: iGEM 2019 Plate2 Well15P
display id: iGEM_2019_Plate2_Well15P
version: 1
owned by: myers
member: BBa_J04421

iGEM 2019 Distribution Plate 2 Well 14D
type: Collection
persistent identity: iGEM 2019 Plate2 Well14D
display id: iGEM_2019_Plate2_Well14D
version: 1
owned by: myers
member: PT7-E

iGEM 2019 Distribution Plate 2 Well 17E
type: Collection
persistent identity: iGEM 2019 Plate2 Well17E
display id: iGEM_2019_Plate2_Well17E
version: 1
owned by: myers
member: BBa_K786003

iGEM 2019 Distribution Plate 2 Well 6G
type: Collection
persistent identity: iGEM 2019 Plate2 Well6G
display id: iGEM_2019_Plate2_Well6G
version: 1
owned by: myers
member: BBa_K876057

iGEM 2019 Distribution Plate 2 Well 24B
type: Collection
persistent identity: iGEM 2019 Plate2 Well24B
display id: iGEM_2019_Plate2_Well24B
version: 1
owned by: myers
member: Adapter

iGEM 2019 Distribution Plate 2 Well 9F
type: Collection
persistent identity: iGEM 2019 Plate2 Well9F
display id: iGEM_2019_Plate2_Well9F
version: 1
owned by: myers
member: BBa_I6030

iGEM 2019 Distribution Plate 2 Well 17A
type: Collection
persistent identity: iGEM 2019 Plate2 Well17A
display id: iGEM_2019_Plate2_Well17A
version: 1
owned by: myers
member: BBa_K759012

iGEM 2019 Distribution Plate 2 Well 5O
type: Collection
persistent identity: iGEM 2019 Plate2 Well5O
display id: iGEM_2019_Plate2_Well5O
version: 1
owned by: myers
member: BBa_K731255

iGEM 2019 Distribution Plate 2 Well 5H
type: Collection
persistent identity: iGEM 2019 Plate2 Well5H
display id: iGEM_2019_Plate2_Well5H
version: 1
owned by: myers
member: lux pR

iGEM 2019 Distribution Plate 2 Well 24J
type: Collection
persistent identity: iGEM 2019 Plate2 Well24J
display id: iGEM_2019_Plate2_Well24J
version: 1
owned by: myers
member: BBa_I714033

iGEM 2019 Distribution Plate 2 Well 3N
type: Collection
persistent identity: iGEM 2019 Plate2 Well3N
display id: iGEM_2019_Plate2_Well3N
version: 1
owned by: myers
member: ecfp

iGEM 2019 Distribution Plate 2 Well 9K
type: Collection
persistent identity: iGEM 2019 Plate2 Well9K
display id: iGEM_2019_Plate2_Well9K
version: 1
owned by: myers
member: BBa_K748003

iGEM 2019 Distribution Plate 2 Well 21G
type: Collection
persistent identity: iGEM 2019 Plate2 Well21G
display id: iGEM_2019_Plate2_Well21G
version: 1
owned by: myers
member: BBa_K849004

iGEM 2019 Distribution Plate 2 Well 13K
type: Collection
persistent identity: iGEM 2019 Plate2 Well13K
display id: iGEM_2019_Plate2_Well13K
version: 1
owned by: myers
member: BBa_K863001

iGEM 2019 Distribution Plate 2 Well 24O
type: Collection
persistent identity: iGEM 2019 Plate2 Well24O
display id: iGEM_2019_Plate2_Well24O
version: 1
owned by: myers
member: BBa_J04450

iGEM 2019 Distribution Plate 2 Well 21D
type: Collection
persistent identity: iGEM 2019 Plate2 Well21D
display id: iGEM_2019_Plate2_Well21D
version: 1
owned by: myers
member: BBa_I732018

iGEM 2019 Distribution Plate 2 Well 11C
type: Collection
persistent identity: iGEM 2019 Plate2 Well11C
display id: iGEM_2019_Plate2_Well11C
version: 1
owned by: myers
member: phaC1-A-B1

iGEM 2019 Distribution Plate 2 Well 7B
type: Collection
persistent identity: iGEM 2019 Plate2 Well7B
display id: iGEM_2019_Plate2_Well7B
version: 1
owned by: myers
member: BBa_S0100

iGEM 2019 Distribution Plate 2 Well 21P
type: Collection
persistent identity: iGEM 2019 Plate2 Well21P
display id: iGEM_2019_Plate2_Well21P
version: 1
owned by: myers
member: BBa_I6211

iGEM 2019 Distribution Plate 2 Well 4A
type: Collection
persistent identity: iGEM 2019 Plate2 Well4A
display id: iGEM_2019_Plate2_Well4A
version: 1
owned by: myers
member: BBa_K773003

iGEM 2019 Distribution Plate 2 Well 15H
type: Collection
persistent identity: iGEM 2019 Plate2 Well15H
display id: iGEM_2019_Plate2_Well15H
version: 1
owned by: myers
member: BBa_J61016

iGEM 2019 Distribution Plate 2 Well 16I
type: Collection
persistent identity: iGEM 2019 Plate2 Well16I
display id: iGEM_2019_Plate2_Well16I
version: 1
owned by: myers
member: BBa_K936020

iGEM 2019 Distribution Plate 2 Well 1F
type: Collection
persistent identity: iGEM 2019 Plate2 Well1F
display id: iGEM_2019_Plate2_Well1F
version: 1
owned by: myers
member: BBa_B0023

iGEM 2019 Distribution Plate 2 Well 13P
type: Collection
persistent identity: iGEM 2019 Plate2 Well13P
display id: iGEM_2019_Plate2_Well13P
version: 1
owned by: myers
member: mRFP1

iGEM 2019 Distribution Plate 2 Well 6A
type: Collection
persistent identity: iGEM 2019 Plate2 Well6A
display id: iGEM_2019_Plate2_Well6A
version: 1
owned by: myers
member: BBa_K876010

iGEM 2019 Distribution Plate 2 Well 2A
type: Collection
persistent identity: iGEM 2019 Plate2 Well2A
display id: iGEM_2019_Plate2_Well2A
version: 1
owned by: myers
member: BBa_K909007

iGEM 2019 Distribution Plate 2 Well 22E
type: Collection
persistent identity: iGEM 2019 Plate2 Well22E
display id: iGEM_2019_Plate2_Well22E
version: 1
owned by: myers
member: BBa_K876068

iGEM 2019 Distribution Plate 2 Well 10L
type: Collection
persistent identity: iGEM 2019 Plate2 Well10L
display id: iGEM_2019_Plate2_Well10L
version: 1
owned by: myers
member: BBa_I746320

iGEM 2019 Distribution Plate 2 Well 19D
type: Collection
persistent identity: iGEM 2019 Plate2 Well19D
display id: iGEM_2019_Plate2_Well19D
version: 1
owned by: myers
member: BBa_M30011

iGEM 2019 Distribution Plate 2 Well 2P
type: Collection
persistent identity: iGEM 2019 Plate2 Well2P
display id: iGEM_2019_Plate2_Well2P
version: 1
owned by: myers
member: BBa_E5503

iGEM 2019 Distribution Plate 2 Well 7A
type: Collection
persistent identity: iGEM 2019 Plate2 Well7A
display id: iGEM_2019_Plate2_Well7A
version: 1
owned by: myers
member: BBa_K731520

iGEM 2019 Distribution Plate 2 Well 5P
type: Collection
persistent identity: iGEM 2019 Plate2 Well5P
display id: iGEM_2019_Plate2_Well5P
version: 1
owned by: myers
member: BBa_E0422

iGEM 2019 Distribution Plate 2 Well 17J
type: Collection
persistent identity: iGEM 2019 Plate2 Well17J
display id: iGEM_2019_Plate2_Well17J
version: 1
owned by: myers
member: BBa_I13541

iGEM 2019 Distribution Plate 2 Well 7F
type: Collection
persistent identity: iGEM 2019 Plate2 Well7F
display id: iGEM_2019_Plate2_Well7F
version: 1
owned by: myers
member: BBa_S0108

iGEM 2019 Distribution Plate 2 Well 22B
type: Collection
persistent identity: iGEM 2019 Plate2 Well22B
display id: iGEM_2019_Plate2_Well22B
version: 1
owned by: myers
member: BBa_B0030

iGEM 2019 Distribution Plate 2 Well 20E
type: Collection
persistent identity: iGEM 2019 Plate2 Well20E
display id: iGEM_2019_Plate2_Well20E
version: 1
owned by: myers
member: BBa_K876009

iGEM 2019 Distribution Plate 2 Well 22C
type: Collection
persistent identity: iGEM 2019 Plate2 Well22C
display id: iGEM_2019_Plate2_Well22C
version: 1
owned by: myers
member: BBa_K876067

iGEM 2019 Distribution Plate 2 Well 14E
type: Collection
persistent identity: iGEM 2019 Plate2 Well14E
display id: iGEM_2019_Plate2_Well14E
version: 1
owned by: myers
member: BBa_J202011

iGEM 2019 Distribution Plate 2 Well 21K
type: Collection
persistent identity: iGEM 2019 Plate2 Well21K
display id: iGEM_2019_Plate2_Well21K
version: 1
owned by: myers
member: BBa_K929002

iGEM 2019 Distribution Plate 2 Well 9I
type: Collection
persistent identity: iGEM 2019 Plate2 Well9I
display id: iGEM_2019_Plate2_Well9I
version: 1
owned by: myers
member: BBa_K850002

iGEM 2019 Distribution Plate 2 Well 4B
type: Collection
persistent identity: iGEM 2019 Plate2 Well4B
display id: iGEM_2019_Plate2_Well4B
version: 1
owned by: myers
member: BBa_E5504

iGEM 2019 Distribution Plate 2 Well 1C
type: Collection
persistent identity: iGEM 2019 Plate2 Well1C
display id: iGEM_2019_Plate2_Well1C
version: 1
owned by: myers
member: BBa_K598014

iGEM 2019 Distribution Plate 2 Well 19C
type: Collection
persistent identity: iGEM 2019 Plate2 Well19C
display id: iGEM_2019_Plate2_Well19C
version: 1
owned by: myers
member: BBa_K822002

iGEM 2019 Distribution Plate 2 Well 2O
type: Collection
persistent identity: iGEM 2019 Plate2 Well2O
display id: iGEM_2019_Plate2_Well2O
version: 1
owned by: myers
member: BBa_K773002

iGEM 2019 Distribution Plate 2 Well 8N
type: Collection
persistent identity: iGEM 2019 Plate2 Well8N
display id: iGEM_2019_Plate2_Well8N
version: 1
owned by: myers
member: BBa_I732074

iGEM 2019 Distribution Plate 2 Well 23B
type: Collection
persistent identity: iGEM 2019 Plate2 Well23B
display id: iGEM_2019_Plate2_Well23B
version: 1
owned by: myers
member: GFP report

iGEM 2019 Distribution Plate 2 Well 4I
type: Collection
persistent identity: iGEM 2019 Plate2 Well4I
display id: iGEM_2019_Plate2_Well4I
version: 1
owned by: myers
member: BBa_K801012

iGEM 2019 Distribution Plate 2 Well 14P
type: Collection
persistent identity: iGEM 2019 Plate2 Well14P
display id: iGEM_2019_Plate2_Well14P
version: 1
owned by: myers
member: BBa_I718015

iGEM 2019 Distribution Plate 2 Well 18N
type: Collection
persistent identity: iGEM 2019 Plate2 Well18N
display id: iGEM_2019_Plate2_Well18N
version: 1
owned by: myers
member: R6K

iGEM 2019 Distribution Plate 2 Well 11P
type: Collection
persistent identity: iGEM 2019 Plate2 Well11P
display id: iGEM_2019_Plate2_Well11P
version: 1
owned by: myers
member: BBa_I6076

iGEM 2019 Distribution Plate 2 Well 6J
type: Collection
persistent identity: iGEM 2019 Plate2 Well6J
display id: iGEM_2019_Plate2_Well6J
version: 1
owned by: myers
member: BBa_I13025

iGEM 2019 Distribution Plate 2 Well 18L
type: Collection
persistent identity: iGEM 2019 Plate2 Well18L
display id: iGEM_2019_Plate2_Well18L
version: 1
owned by: myers
member: BBa_I13001

iGEM 2019 Distribution Plate 2 Well 11G
type: Collection
persistent identity: iGEM 2019 Plate2 Well11G
display id: iGEM_2019_Plate2_Well11G
version: 1
owned by: myers
member: BBa_K733002

iGEM 2019 Distribution Plate 2 Well 11F
type: Collection
persistent identity: iGEM 2019 Plate2 Well11F
display id: iGEM_2019_Plate2_Well11F
version: 1
owned by: myers
member: BBa_I6056

iGEM 2019 Distribution Plate 2 Well 13O
type: Collection
persistent identity: iGEM 2019 Plate2 Well13O
display id: iGEM_2019_Plate2_Well13O
version: 1
owned by: myers
member: BBa_K863006

iGEM 2019 Distribution Plate 2 Well 23F
type: Collection
persistent identity: iGEM 2019 Plate2 Well23F
display id: iGEM_2019_Plate2_Well23F
version: 1
owned by: myers
member: BBa_S01003

iGEM 2019 Distribution Plate 2 Well 3C
type: Collection
persistent identity: iGEM 2019 Plate2 Well3C
display id: iGEM_2019_Plate2_Well3C
version: 1
owned by: myers
member: BBa_K519030

iGEM 2019 Distribution Plate 2 Well 13G
type: Collection
persistent identity: iGEM 2019 Plate2 Well13G
display id: iGEM_2019_Plate2_Well13G
version: 1
owned by: myers
member: BBa_K769022

iGEM 2019 Distribution Plate 2 Well 13D
type: Collection
persistent identity: iGEM 2019 Plate2 Well13D
display id: iGEM_2019_Plate2_Well13D
version: 1
owned by: myers
member: BBa_I6084

iGEM 2019 Distribution Plate 2 Well 17H
type: Collection
persistent identity: iGEM 2019 Plate2 Well17H
display id: iGEM_2019_Plate2_Well17H
version: 1
owned by: myers
member: BBa_I13457

iGEM 2019 Distribution Plate 2 Well 17N
type: Collection
persistent identity: iGEM 2019 Plate2 Well17N
display id: iGEM_2019_Plate2_Well17N
version: 1
owned by: myers
member: BBa_I13542

iGEM 2019 Distribution Plate 2 Well 9D
type: Collection
persistent identity: iGEM 2019 Plate2 Well9D
display id: iGEM_2019_Plate2_Well9D
version: 1
owned by: myers
member: BBa_P0440

iGEM 2019 Distribution Plate 2 Well 22O
type: Collection
persistent identity: iGEM 2019 Plate2 Well22O
display id: iGEM_2019_Plate2_Well22O
version: 1
owned by: myers
member: BBa_K737051

iGEM 2019 Distribution Plate 2 Well 6F
type: Collection
persistent identity: iGEM 2019 Plate2 Well6F
display id: iGEM_2019_Plate2_Well6F
version: 1
owned by: myers
member: RhlR+C4

iGEM 2019 Distribution Plate 2 Well 8M
type: Collection
persistent identity: iGEM 2019 Plate2 Well8M
display id: iGEM_2019_Plate2_Well8M
version: 1
owned by: myers
member: BBa_K779504

iGEM 2019 Distribution Plate 2 Well 7K
type: Collection
persistent identity: iGEM 2019 Plate2 Well7K
display id: iGEM_2019_Plate2_Well7K
version: 1
owned by: myers
member: Operon2

iGEM 2019 Distribution Plate 2 Well 17K
type: Collection
persistent identity: iGEM 2019 Plate2 Well17K
display id: iGEM_2019_Plate2_Well17K
version: 1
owned by: myers
member: BBa_K861110

iGEM 2019 Distribution Plate 2 Well 18K
type: Collection
persistent identity: iGEM 2019 Plate2 Well18K
display id: iGEM_2019_Plate2_Well18K
version: 1
owned by: myers
member: BBa_K801072

iGEM 2019 Distribution Plate 2 Well 14G
type: Collection
persistent identity: iGEM 2019 Plate2 Well14G
display id: iGEM_2019_Plate2_Well14G
version: 1
owned by: myers
member: BBa_J202012

iGEM 2019 Distribution Plate 2 Well 5L
type: Collection
persistent identity: iGEM 2019 Plate2 Well5L
display id: iGEM_2019_Plate2_Well5L
version: 1
owned by: myers
member: cI+luxR

iGEM 2019 Distribution Plate 2 Well 13C
type: Collection
persistent identity: iGEM 2019 Plate2 Well13C
display id: iGEM_2019_Plate2_Well13C
version: 1
owned by: myers
member: BBa_K763005

iGEM 2019 Distribution Plate 2 Well 15E
type: Collection
persistent identity: iGEM 2019 Plate2 Well15E
display id: iGEM_2019_Plate2_Well15E
version: 1
owned by: myers
member: BBa_K863020

iGEM 2019 Distribution Plate 2 Well 20B
type: Collection
persistent identity: iGEM 2019 Plate2 Well20B
display id: iGEM_2019_Plate2_Well20B
version: 1
owned by: myers
member: BBa_I13002

iGEM 2019 Distribution Plate 2 Well 2E
type: Collection
persistent identity: iGEM 2019 Plate2 Well2E
display id: iGEM_2019_Plate2_Well2E
version: 1
owned by: myers
member: BBa_K777117

iGEM 2019 Distribution Plate 2 Well 2F
type: Collection
persistent identity: iGEM 2019 Plate2 Well2F
display id: iGEM_2019_Plate2_Well2F
version: 1
owned by: myers
member: ECFP

iGEM 2019 Distribution Plate 2 Well 12B
type: Collection
persistent identity: iGEM 2019 Plate2 Well12B
display id: iGEM_2019_Plate2_Well12B
version: 1
owned by: myers
member: BBa_I715032

iGEM 2019 Distribution Plate 2 Well 5K
type: Collection
persistent identity: iGEM 2019 Plate2 Well5K
display id: iGEM_2019_Plate2_Well5K
version: 1
owned by: myers
member: araC-CysEm

iGEM 2019 Distribution Plate 2 Well 18I
type: Collection
persistent identity: iGEM 2019 Plate2 Well18I
display id: iGEM_2019_Plate2_Well18I
version: 1
owned by: myers
member: BBa_K801071

iGEM 2019 Distribution Plate 2 Well 9B
type: Collection
persistent identity: iGEM 2019 Plate2 Well9B
display id: iGEM_2019_Plate2_Well9B
version: 1
owned by: myers
member: BBa_P0153

iGEM 2019 Distribution Plate 2 Well 23A
type: Collection
persistent identity: iGEM 2019 Plate2 Well23A
display id: iGEM_2019_Plate2_Well23A
version: 1
owned by: myers
member: BBa_K917014

iGEM 2019 Distribution Plate 2 Well 10E
type: Collection
persistent identity: iGEM 2019 Plate2 Well10E
display id: iGEM_2019_Plate2_Well10E
version: 1
owned by: myers
member: BBa_K737033

iGEM 2019 Distribution Plate 2 Well 18G
type: Collection
persistent identity: iGEM 2019 Plate2 Well18G
display id: iGEM_2019_Plate2_Well18G
version: 1
owned by: myers
member: BBa_K801070

iGEM 2019 Distribution Plate 2 Well 12A
type: Collection
persistent identity: iGEM 2019 Plate2 Well12A
display id: iGEM_2019_Plate2_Well12A
version: 1
owned by: myers
member: BBa_J202000

iGEM 2019 Distribution Plate 2 Well 16K
type: Collection
persistent identity: iGEM 2019 Plate2 Well16K
display id: iGEM_2019_Plate2_Well16K
version: 1
owned by: myers
member: BBa_K876002

iGEM 2019 Distribution Plate 2 Well 20J
type: Collection
persistent identity: iGEM 2019 Plate2 Well20J
display id: iGEM_2019_Plate2_Well20J
version: 1
owned by: myers
member: BBa_P0151

iGEM 2019 Distribution Plate 2 Well 18E
type: Collection
persistent identity: iGEM 2019 Plate2 Well18E
display id: iGEM_2019_Plate2_Well18E
version: 1
owned by: myers
member: BBa_K847101

iGEM 2019 Distribution Plate 2 Well 2D
type: Collection
persistent identity: iGEM 2019 Plate2 Well2D
display id: iGEM_2019_Plate2_Well2D
version: 1
owned by: myers
member: EYFP

iGEM 2019 Distribution Plate 2 Well 1O
type: Collection
persistent identity: iGEM 2019 Plate2 Well1O
display id: iGEM_2019_Plate2_Well1O
version: 1
owned by: myers
member: BBa_K567018

iGEM 2019 Distribution Plate 2 Well 9J
type: Collection
persistent identity: iGEM 2019 Plate2 Well9J
display id: iGEM_2019_Plate2_Well9J
version: 1
owned by: myers
member: BBa_I6032

iGEM 2019 Distribution Plate 2 Well 7H
type: Collection
persistent identity: iGEM 2019 Plate2 Well7H
display id: iGEM_2019_Plate2_Well7H
version: 1
owned by: myers
member: BBa_S0109

iGEM 2019 Distribution Plate 2 Well 23I
type: Collection
persistent identity: iGEM 2019 Plate2 Well23I
display id: iGEM_2019_Plate2_Well23I
version: 1
owned by: myers
member: BBa_K737004

iGEM 2019 Distribution Plate 2 Well 18A
type: Collection
persistent identity: iGEM 2019 Plate2 Well18A
display id: iGEM_2019_Plate2_Well18A
version: 1
owned by: myers
member: BBa_K909006

iGEM 2019 Distribution Plate 2 Well 12L
type: Collection
persistent identity: iGEM 2019 Plate2 Well12L
display id: iGEM_2019_Plate2_Well12L
version: 1
owned by: myers
member: BBa_I716208

iGEM 2019 Distribution Plate 2 Well 24P
type: Collection
persistent identity: iGEM 2019 Plate2 Well24P
display id: iGEM_2019_Plate2_Well24P
version: 1
owned by: myers
member: BBa_J04450

iGEM 2019 Distribution Plate 2 Well 17P
type: Collection
persistent identity: iGEM 2019 Plate2 Well17P
display id: iGEM_2019_Plate2_Well17P
version: 1
owned by: myers
member: BBa_I7108

iGEM 2019 Distribution Plate 2 Well 9C
type: Collection
persistent identity: iGEM 2019 Plate2 Well9C
display id: iGEM_2019_Plate2_Well9C
version: 1
owned by: myers
member: BBa_K808034

iGEM 2019 Distribution Plate 2 Well 20O
type: Collection
persistent identity: iGEM 2019 Plate2 Well20O
display id: iGEM_2019_Plate2_Well20O
version: 1
owned by: myers
member: BBa_K876060

iGEM 2019 Distribution Plate 2 Well 9A
type: Collection
persistent identity: iGEM 2019 Plate2 Well9A
display id: iGEM_2019_Plate2_Well9A
version: 1
owned by: myers
member: BBa_K808033

iGEM 2019 Distribution Plate 2 Well 8K
type: Collection
persistent identity: iGEM 2019 Plate2 Well8K
display id: iGEM_2019_Plate2_Well8K
version: 1
owned by: myers
member: BBa_K779503

iGEM 2019 Distribution Plate 2 Well 11K
type: Collection
persistent identity: iGEM 2019 Plate2 Well11K
display id: iGEM_2019_Plate2_Well11K
version: 1
owned by: myers
member: BBa_K750000

iGEM 2019 Distribution Plate 2 Well 1N
type: Collection
persistent identity: iGEM 2019 Plate2 Well1N
display id: iGEM_2019_Plate2_Well1N
version: 1
owned by: myers
member: lacI

iGEM 2019 Distribution Plate 2 Well 14C
type: Collection
persistent identity: iGEM 2019 Plate2 Well14C
display id: iGEM_2019_Plate2_Well14C
version: 1
owned by: myers
member: BBa_J202010

iGEM 2019 Distribution Plate 2 Well 8F
type: Collection
persistent identity: iGEM 2019 Plate2 Well8F
display id: iGEM_2019_Plate2_Well8F
version: 1
owned by: myers
member: BBa_I13450

iGEM 2019 Distribution Plate 2 Well 8L
type: Collection
persistent identity: iGEM 2019 Plate2 Well8L
display id: iGEM_2019_Plate2_Well8L
version: 1
owned by: myers
member: BBa_I732807

iGEM 2019 Distribution Plate 2 Well 1P
type: Collection
persistent identity: iGEM 2019 Plate2 Well1P
display id: iGEM_2019_Plate2_Well1P
version: 1
owned by: myers
member: tetR

iGEM 2019 Distribution Plate 2 Well 7P
type: Collection
persistent identity: iGEM 2019 Plate2 Well7P
display id: iGEM_2019_Plate2_Well7P
version: 1
owned by: myers
member: BBa_P0152

iGEM 2019 Distribution Plate 2 Well 8P
type: Collection
persistent identity: iGEM 2019 Plate2 Well8P
display id: iGEM_2019_Plate2_Well8P
version: 1
owned by: myers
member: BBa_I732812

iGEM 2019 Distribution Plate 2 Well 7M
type: Collection
persistent identity: iGEM 2019 Plate2 Well7M
display id: iGEM_2019_Plate2_Well7M
version: 1
owned by: myers
member: BBa_K808012

iGEM 2019 Distribution Plate 2 Well 13M
type: Collection
persistent identity: iGEM 2019 Plate2 Well13M
display id: iGEM_2019_Plate2_Well13M
version: 1
owned by: myers
member: BBa_K863005

iGEM 2019 Distribution Plate 2 Well 16E
type: Collection
persistent identity: iGEM 2019 Plate2 Well16E
display id: iGEM_2019_Plate2_Well16E
version: 1
owned by: myers
member: GFP-AID

iGEM 2019 Distribution Plate 2 Well 19O
type: Collection
persistent identity: iGEM 2019 Plate2 Well19O
display id: iGEM_2019_Plate2_Well19O
version: 1
owned by: myers
member: BBa_K775009

iGEM 2019 Distribution Plate 2 Well 16D
type: Collection
persistent identity: iGEM 2019 Plate2 Well16D
display id: iGEM_2019_Plate2_Well16D
version: 1
owned by: myers
member: BBa_S03857

iGEM 2019 Distribution Plate 2 Well 20N
type: Collection
persistent identity: iGEM 2019 Plate2 Well20N
display id: iGEM_2019_Plate2_Well20N
version: 1
owned by: myers
member: cI lam

iGEM 2019 Distribution Plate 2 Well 11J
type: Collection
persistent identity: iGEM 2019 Plate2 Well11J
display id: iGEM_2019_Plate2_Well11J
version: 1
owned by: myers
member: BBa_I6064

iGEM 2019 Distribution Plate 2 Well 10M
type: Collection
persistent identity: iGEM 2019 Plate2 Well10M
display id: iGEM_2019_Plate2_Well10M
version: 1
owned by: myers
member: BBa_K747095

iGEM 2019 Distribution Plate 2 Well 24D
type: Collection
persistent identity: iGEM 2019 Plate2 Well24D
display id: iGEM_2019_Plate2_Well24D
version: 1
owned by: myers
member: LRLa

iGEM 2019 Distribution Plate 2 Well 21I
type: Collection
persistent identity: iGEM 2019 Plate2 Well21I
display id: iGEM_2019_Plate2_Well21I
version: 1
owned by: myers
member: CMV AID pA

iGEM 2019 Distribution Plate 2 Well 4D
type: Collection
persistent identity: iGEM 2019 Plate2 Well4D
display id: iGEM_2019_Plate2_Well4D
version: 1
owned by: myers
member: BBa_S01022

iGEM 2019 Distribution Plate 2 Well 16N
type: Collection
persistent identity: iGEM 2019 Plate2 Well16N
display id: iGEM_2019_Plate2_Well16N
version: 1
owned by: myers
member: BBa_I13452

iGEM 2019 Distribution Plate 2 Well 8G
type: Collection
persistent identity: iGEM 2019 Plate2 Well8G
display id: iGEM_2019_Plate2_Well8G
version: 1
owned by: myers
member: BBa_K779500

iGEM 2019 Distribution Plate 2 Well 10B
type: Collection
persistent identity: iGEM 2019 Plate2 Well10B
display id: iGEM_2019_Plate2_Well10B
version: 1
owned by: myers
member: BBa_I732912

iGEM 2019 Distribution Plate 2 Well 19J
type: Collection
persistent identity: iGEM 2019 Plate2 Well19J
display id: iGEM_2019_Plate2_Well19J
version: 1
owned by: myers
member: BBa_J01142

iGEM 2019 Distribution Plate 2 Well 17D
type: Collection
persistent identity: iGEM 2019 Plate2 Well17D
display id: iGEM_2019_Plate2_Well17D
version: 1
owned by: myers
member: mCherry

iGEM 2019 Distribution Plate 2 Well 1A
type: Collection
persistent identity: iGEM 2019 Plate2 Well1A
display id: iGEM_2019_Plate2_Well1A
version: 1
owned by: myers
member: BBa_K649303

iGEM 2019 Distribution Plate 2 Well 19M
type: Collection
persistent identity: iGEM 2019 Plate2 Well19M
display id: iGEM_2019_Plate2_Well19M
version: 1
owned by: myers
member: BBa_K895005

iGEM 2019 Distribution Plate 2 Well 3J
type: Collection
persistent identity: iGEM 2019 Plate2 Well3J
display id: iGEM_2019_Plate2_Well3J
version: 1
owned by: myers
member: luxI

iGEM 2019 Distribution Plate 2 Well 21C
type: Collection
persistent identity: iGEM 2019 Plate2 Well21C
display id: iGEM_2019_Plate2_Well21C
version: 1
owned by: myers
member: HMG-CoA-R

iGEM 2019 Distribution Plate 2 Well 2I
type: Collection
persistent identity: iGEM 2019 Plate2 Well2I
display id: iGEM_2019_Plate2_Well2I
version: 1
owned by: myers
member: BBa_K936013

iGEM 2019 Distribution Plate 2 Well 3L
type: Collection
persistent identity: iGEM 2019 Plate2 Well3L
display id: iGEM_2019_Plate2_Well3L
version: 1
owned by: myers
member: luxr

iGEM 2019 Distribution Plate 2 Well 22F
type: Collection
persistent identity: iGEM 2019 Plate2 Well22F
display id: iGEM_2019_Plate2_Well22F
version: 1
owned by: myers
member: SV40 NLS

iGEM 2019 Distribution Plate 2 Well 14M
type: Collection
persistent identity: iGEM 2019 Plate2 Well14M
display id: iGEM_2019_Plate2_Well14M
version: 1
owned by: myers
member: BBa_K909009

iGEM 2019 Distribution Plate 2 Well 8E
type: Collection
persistent identity: iGEM 2019 Plate2 Well8E
display id: iGEM_2019_Plate2_Well8E
version: 1
owned by: myers
member: BBa_K844008

iGEM 2019 Distribution Plate 2 Well 19H
type: Collection
persistent identity: iGEM 2019 Plate2 Well19H
display id: iGEM_2019_Plate2_Well19H
version: 1
owned by: myers
member: BBa_J01141

iGEM 2019 Distribution Plate 2 Well 7N
type: Collection
persistent identity: iGEM 2019 Plate2 Well7N
display id: iGEM_2019_Plate2_Well7N
version: 1
owned by: myers
member: BBa_S0117

iGEM 2019 Distribution Plate 2 Well 1H
type: Collection
persistent identity: iGEM 2019 Plate2 Well1H
display id: iGEM_2019_Plate2_Well1H
version: 1
owned by: myers
member: BBa_B0031

iGEM 2019 Distribution Plate 2 Well 19E
type: Collection
persistent identity: iGEM 2019 Plate2 Well19E
display id: iGEM_2019_Plate2_Well19E
version: 1
owned by: myers
member: BBa_K863204

iGEM 2019 Distribution Plate 2 Well 18M
type: Collection
persistent identity: iGEM 2019 Plate2 Well18M
display id: iGEM_2019_Plate2_Well18M
version: 1
owned by: myers
member: BBa_K801092

iGEM 2019 Distribution Plate 2 Well 15M
type: Collection
persistent identity: iGEM 2019 Plate2 Well15M
display id: iGEM_2019_Plate2_Well15M
version: 1
owned by: myers
member: BBa_K733012

iGEM 2019 Distribution Plate 2 Well 8A
type: Collection
persistent identity: iGEM 2019 Plate2 Well8A
display id: iGEM_2019_Plate2_Well8A
version: 1
owned by: myers
member: BBa_K844006

iGEM 2019 Distribution Plate 2 Well 15L
type: Collection
persistent identity: iGEM 2019 Plate2 Well15L
display id: iGEM_2019_Plate2_Well15L
version: 1
owned by: myers
member: BBa_R2109

iGEM 2019 Distribution Plate 2 Well 10H
type: Collection
persistent identity: iGEM 2019 Plate2 Well10H
display id: iGEM_2019_Plate2_Well10H
version: 1
owned by: myers
member: BBa_I732801

iGEM 2019 Distribution Plate 2 Well 10O
type: Collection
persistent identity: iGEM 2019 Plate2 Well10O
display id: iGEM_2019_Plate2_Well10O
version: 1
owned by: myers
member: BBa_K911003

iGEM 2019 Distribution Plate 2 Well 10C
type: Collection
persistent identity: iGEM 2019 Plate2 Well10C
display id: iGEM_2019_Plate2_Well10C
version: 1
owned by: myers
member: BBa_K737032

iGEM 2019 Distribution Plate 2 Well 22G
type: Collection
persistent identity: iGEM 2019 Plate2 Well22G
display id: iGEM_2019_Plate2_Well22G
version: 1
owned by: myers
member: BBa_K876073

iGEM 2019 Distribution Plate 2 Well 6H
type: Collection
persistent identity: iGEM 2019 Plate2 Well6H
display id: iGEM_2019_Plate2_Well6H
version: 1
owned by: myers
member: AraC

iGEM 2019 Distribution Plate 2 Well 13N
type: Collection
persistent identity: iGEM 2019 Plate2 Well13N
display id: iGEM_2019_Plate2_Well13N
version: 1
owned by: myers
member: BBa_I6105

iGEM 2019 Distribution Plate 2 Well 21J
type: Collection
persistent identity: iGEM 2019 Plate2 Well21J
display id: iGEM_2019_Plate2_Well21J
version: 1
owned by: myers
member: BBa_I6107

iGEM 2019 Distribution Plate 2 Well 3G
type: Collection
persistent identity: iGEM 2019 Plate2 Well3G
display id: iGEM_2019_Plate2_Well3G
version: 1
owned by: myers
member: BBa_K574006

iGEM 2019 Distribution Plate 2 Well 21F
type: Collection
persistent identity: iGEM 2019 Plate2 Well21F
display id: iGEM_2019_Plate2_Well21F
version: 1
owned by: myers
member: BBa_I732810

iGEM 2019 Distribution Plate 2 Well 22P
type: Collection
persistent identity: iGEM 2019 Plate2 Well22P
display id: iGEM_2019_Plate2_Well22P
version: 1
owned by: myers
member: OriTR

iGEM 2019 Distribution Plate 2 Well 17I
type: Collection
persistent identity: iGEM 2019 Plate2 Well17I
display id: iGEM_2019_Plate2_Well17I
version: 1
owned by: myers
member: BBa_K895002

iGEM 2019 Distribution Plate 2 Well 13H
type: Collection
persistent identity: iGEM 2019 Plate2 Well13H
display id: iGEM_2019_Plate2_Well13H
version: 1
owned by: myers
member: BBa_I6101

iGEM 2019 Distribution Plate 2 Well 21O
type: Collection
persistent identity: iGEM 2019 Plate2 Well21O
display id: iGEM_2019_Plate2_Well21O
version: 1
owned by: myers
member: BBa_K864444

iGEM 2019 Distribution Plate 2 Well 19B
type: Collection
persistent identity: iGEM 2019 Plate2 Well19B
display id: iGEM_2019_Plate2_Well19B
version: 1
owned by: myers
member: BBa_I13528

iGEM 2019 Distribution Plate 2 Well 17L
type: Collection
persistent identity: iGEM 2019 Plate2 Well17L
display id: iGEM_2019_Plate2_Well17L
version: 1
owned by: myers
member: BBa_I13523

iGEM 2019 Distribution Plate 2 Well 16H
type: Collection
persistent identity: iGEM 2019 Plate2 Well16H
display id: iGEM_2019_Plate2_Well16H
version: 1
owned by: myers
member: BBa_S03859

iGEM 2019 Distribution Plate 2 Well 13J
type: Collection
persistent identity: iGEM 2019 Plate2 Well13J
display id: iGEM_2019_Plate2_Well13J
version: 1
owned by: myers
member: BBa_I6102

iGEM 2019 Distribution Plate 2 Well 19A
type: Collection
persistent identity: iGEM 2019 Plate2 Well19A
display id: iGEM_2019_Plate2_Well19A
version: 1
owned by: myers
member: BBa_K741020

iGEM 2019 Distribution Plate 2 Well 11N
type: Collection
persistent identity: iGEM 2019 Plate2 Well11N
display id: iGEM_2019_Plate2_Well11N
version: 1
owned by: myers
member: BBa_I6070

iGEM 2019 Distribution Plate 2 Well 7L
type: Collection
persistent identity: iGEM 2019 Plate2 Well7L
display id: iGEM_2019_Plate2_Well7L
version: 1
owned by: myers
member: BBa_S0116

iGEM 2019 Distribution Plate 2 Well 7C
type: Collection
persistent identity: iGEM 2019 Plate2 Well7C
display id: iGEM_2019_Plate2_Well7C
version: 1
owned by: myers
member: BBa_K808032

iGEM 2019 Distribution Plate 2
type: Collection
persistent identity: iGEM 2019 Plate2
display id: iGEM_2019_Plate2
version: 1
owned by: myers
member: BBa_K759012
member: BBa_K812032
member: BBa_I13202
member: iGEM 2019 Distribution Plate 2 Well 20B
member: iGEM 2019 Distribution Plate 2 Well 1N
member: BBa_I6108
member: BBa_J202003
member: iGEM 2019 Distribution Plate 2 Well 9E
member: BBa_K909006
member: iGEM 2019 Distribution Plate 2 Well 3E
member: iGEM 2019 Distribution Plate 2 Well 6E
member: TD1.0
member: iGEM 2019 Distribution Plate 2 Well 10G
member: iGEM 2019 Distribution Plate 2 Well 4N
member: iGEM 2019 Distribution Plate 2 Well 23B
member: BBa_K649303
member: iGEM 2019 Distribution Plate 2 Well 14P
member: iGEM 2019 Distribution Plate 2 Well 11P
member: iGEM 2019 Distribution Plate 2 Well 13G
member: iGEM 2019 Distribution Plate 2 Well 16G
member: iGEM 2019 Distribution Plate 2 Well 19G
member: BBa_K953002
member: iGEM 2019 Distribution Plate 2 Well 21K
member: BBa_I13500
member: BBa_I7108
member: BBa_K775003
member: BBa_K784013
member: iGEM 2019 Distribution Plate 2 Well 22F
member: BBa_I6084
member: BBa_B0023
member: OmpR
member: araC-CysEm
member: iGEM 2019 Distribution Plate 2 Well 18K
member: iGEM 2019 Distribution Plate 2 Well 12K
member: iGEM 2019 Distribution Plate 2 Well 15K
member: BBa_S0117
member: iGEM 2019 Distribution Plate 2 Well 1A
member: BBa_K773003
member: iGEM 2019 Distribution Plate 2 Well 4A
member: iGEM 2019 Distribution Plate 2 Well 7A
member: iGEM 2019 Distribution Plate 2 Well 17P
member: iGEM 2019 Distribution Plate 2 Well 20O
member: BBa_K779503
member: BBa_I6060
member: iGEM 2019 Distribution Plate 2 Well 23O
member: BBa_K769022
member: BBa_S03154
member: iGEM 2019 Distribution Plate 2 Well 24J
member: otsA
member: iGEM 2019 Distribution Plate 2 Well 17O
member: iGEM 2019 Distribution Plate 2 Well 21J
member: BBa_K773004
member: BBa_I6030
member: BBa_K817000
member: BBa_K929002
member: iGEM 2019 Distribution Plate 2 Well 7M
member: iGEM 2019 Distribution Plate 2 Well 20P
member: iGEM 2019 Distribution Plate 2 Well 4M
member: fhlA
member: LasR+PAI
member: iGEM 2019 Distribution Plate 2 Well 23P
member: BBa_K775002
member: BBa_S03119
member: BBa_J202004
member: iGEM 2019 Distribution Plate 2 Well 13F
member: BBa_K876000
member: iGEM 2019 Distribution Plate 2 Well 18L
member: iGEM 2019 Distribution Plate 2 Well 15L
member: iGEM 2019 Distribution Plate 2 Well 1M
member: iGEM 2019 Distribution Plate 2 Well 12L
member: BBa_K808014
member: HMG-CoA-R
member: luxr

iGEM 2019 Distribution Plate 2 Well 15B
type: Collection
persistent identity: iGEM 2019 Plate2 Well15B
display id: iGEM_2019_Plate2_Well15B
version: 1
owned by: myers
member: BBa_I13519

iGEM 2019 Distribution Plate 2 Well 12C
type: Collection
persistent identity: iGEM 2019 Plate2 Well12C
display id: iGEM_2019_Plate2_Well12C
version: 1
owned by: myers
member: BBa_J202002

iGEM 2019 Distribution Plate 2 Well 22H
type: Collection
persistent identity: iGEM 2019 Plate2 Well22H
display id: iGEM_2019_Plate2_Well22H
version: 1
owned by: myers
member: BBa_S03518

iGEM 2019 Distribution Plate 2 Well 3M
type: Collection
persistent identity: iGEM 2019 Plate2 Well3M
display id: iGEM_2019_Plate2_Well3M
version: 1
owned by: myers
member: trkD

iGEM 2019 Distribution Plate 2 Well 1B
type: Collection
persistent identity: iGEM 2019 Plate2 Well1B
display id: iGEM_2019_Plate2_Well1B
version: 1
owned by: myers
member: BBa_B0012

iGEM 2019 Distribution Plate 2 Well 9M
type: Collection
persistent identity: iGEM 2019 Plate2 Well9M
display id: iGEM_2019_Plate2_Well9M
version: 1
owned by: myers
member: BBa_S05051

iGEM 2019 Distribution Plate 2 Well 12D
type: Collection
persistent identity: iGEM 2019 Plate2 Well12D
display id: iGEM_2019_Plate2_Well12D
version: 1
owned by: myers
member: BBa_I714031

iGEM 2019 Distribution Plate 2 Well 4F
type: Collection
persistent identity: iGEM 2019 Plate2 Well4F
display id: iGEM_2019_Plate2_Well4F
version: 1
owned by: myers
member: OmpR

iGEM 2019 Distribution Plate 2 Well 20C
type: Collection
persistent identity: iGEM 2019 Plate2 Well20C
display id: iGEM_2019_Plate2_Well20C
version: 1
owned by: myers
member: BBa_K902059

iGEM 2019 Distribution Plate 2 Well 11E
type: Collection
persistent identity: iGEM 2019 Plate2 Well11E
display id: iGEM_2019_Plate2_Well11E
version: 1
owned by: myers
member: BBa_K934023

iGEM 2019 Distribution Plate 2 Well 21M
type: Collection
persistent identity: iGEM 2019 Plate2 Well21M
display id: iGEM_2019_Plate2_Well21M
version: 1
owned by: myers
member: BBa_K864680

iGEM 2019 Distribution Plate 2 Well 17F
type: Collection
persistent identity: iGEM 2019 Plate2 Well17F
display id: iGEM_2019_Plate2_Well17F
version: 1
owned by: myers
member: BBa_J06602

iGEM 2019 Distribution Plate 2 Well 17G
type: Collection
persistent identity: iGEM 2019 Plate2 Well17G
display id: iGEM_2019_Plate2_Well17G
version: 1
owned by: myers
member: BBa_K863021

iGEM 2019 Distribution Plate 2 Well 19L
type: Collection
persistent identity: iGEM 2019 Plate2 Well19L
display id: iGEM_2019_Plate2_Well19L
version: 1
owned by: myers
member: BBa_I732095

iGEM 2019 Distribution Plate 2 Well 15K
type: Collection
persistent identity: iGEM 2019 Plate2 Well15K
display id: iGEM_2019_Plate2_Well15K
version: 1
owned by: myers
member: Bxb1_Int

iGEM 2019 Distribution Plate 2 Well 12F
type: Collection
persistent identity: iGEM 2019 Plate2 Well12F
display id: iGEM_2019_Plate2_Well12F
version: 1
owned by: myers
member: BBa_I714044

iGEM 2019 Distribution Plate 2 Well 2B
type: Collection
persistent identity: iGEM 2019 Plate2 Well2B
display id: iGEM_2019_Plate2_Well2B
version: 1
owned by: myers
member: BBa_S03156

iGEM 2019 Distribution Plate 2 Well 21N
type: Collection
persistent identity: iGEM 2019 Plate2 Well21N
display id: iGEM_2019_Plate2_Well21N
version: 1
owned by: myers
member: BBa_I6207

iGEM 2019 Distribution Plate 2 Well 23J
type: Collection
persistent identity: iGEM 2019 Plate2 Well23J
display id: iGEM_2019_Plate2_Well23J
version: 1
owned by: myers
member: BBa_C0261

iGEM 2019 Distribution Plate 2 Well 3O
type: Collection
persistent identity: iGEM 2019 Plate2 Well3O
display id: iGEM_2019_Plate2_Well3O
version: 1
owned by: myers
member: BBa_K775000

iGEM 2019 Distribution Plate 2 Well 24L
type: Collection
persistent identity: iGEM 2019 Plate2 Well24L
display id: iGEM_2019_Plate2_Well24L
version: 1
owned by: myers
member: pLambda+RF

iGEM 2019 Distribution Plate 2 Well 16F
type: Collection
persistent identity: iGEM 2019 Plate2 Well16F
display id: iGEM_2019_Plate2_Well16F
version: 1
owned by: myers
member: BBa_S03858

iGEM 2019 Distribution Plate 2 Well 9G
type: Collection
persistent identity: iGEM 2019 Plate2 Well9G
display id: iGEM_2019_Plate2_Well9G
version: 1
owned by: myers
member: BBa_K850000

iGEM 2019 Distribution Plate 2 Well 22K
type: Collection
persistent identity: iGEM 2019 Plate2 Well22K
display id: iGEM_2019_Plate2_Well22K
version: 1
owned by: myers
member: BBa_K737007

iGEM 2019 Distribution Plate 2 Well 13L
type: Collection
persistent identity: iGEM 2019 Plate2 Well13L
display id: iGEM_2019_Plate2_Well13L
version: 1
owned by: myers
member: BBa_I6103

iGEM 2019 Distribution Plate 2 Well 18B
type: Collection
persistent identity: iGEM 2019 Plate2 Well18B
display id: iGEM_2019_Plate2_Well18B
version: 1
owned by: myers
member: mRFP1

iGEM 2019 Distribution Plate 2 Well 9L
type: Collection
persistent identity: iGEM 2019 Plate2 Well9L
display id: iGEM_2019_Plate2_Well9L
version: 1
owned by: myers
member: BBa_I6040

iGEM 2019 Distribution Plate 2 Well 7O
type: Collection
persistent identity: iGEM 2019 Plate2 Well7O
display id: iGEM_2019_Plate2_Well7O
version: 1
owned by: myers
member: BBa_K808014

iGEM 2019 Distribution Plate 2 Well 3B
type: Collection
persistent identity: iGEM 2019 Plate2 Well3B
display id: iGEM_2019_Plate2_Well3B
version: 1
owned by: myers
member: cI lam

iGEM 2019 Distribution Plate 2 Well 5F
type: Collection
persistent identity: iGEM 2019 Plate2 Well5F
display id: iGEM_2019_Plate2_Well5F
version: 1
owned by: myers
member: p(tetR)

iGEM 2019 Distribution Plate 2 Well 3E
type: Collection
persistent identity: iGEM 2019 Plate2 Well3E
display id: iGEM_2019_Plate2_Well3E
version: 1
owned by: myers
member: BBa_K574005

iGEM 2019 Distribution Plate 2 Well 21L
type: Collection
persistent identity: iGEM 2019 Plate2 Well21L
display id: iGEM_2019_Plate2_Well21L
version: 1
owned by: myers
member: BBa_I6108

iGEM 2019 Distribution Plate 2 Well 21A
type: Collection
persistent identity: iGEM 2019 Plate2 Well21A
display id: iGEM_2019_Plate2_Well21A
version: 1
owned by: myers
member: BBa_K775010

iGEM 2019 Distribution Plate 2 Well 8I
type: Collection
persistent identity: iGEM 2019 Plate2 Well8I
display id: iGEM_2019_Plate2_Well8I
version: 1
owned by: myers
member: BBa_K779501

iGEM 2019 Distribution Plate 2 Well 1G
type: Collection
persistent identity: iGEM 2019 Plate2 Well1G
display id: iGEM_2019_Plate2_Well1G
version: 1
owned by: myers
member: BBa_K598020

iGEM 2019 Distribution Plate 2 Well 2K
type: Collection
persistent identity: iGEM 2019 Plate2 Well2K
display id: iGEM_2019_Plate2_Well2K
version: 1
owned by: myers
member: BBa_K936000

iGEM 2019 Distribution Plate 2 Well 2C
type: Collection
persistent identity: iGEM 2019 Plate2 Well2C
display id: iGEM_2019_Plate2_Well2C
version: 1
owned by: myers
member: BBa_K777113

iGEM 2019 Distribution Plate 2 Well 10A
type: Collection
persistent identity: iGEM 2019 Plate2 Well10A
display id: iGEM_2019_Plate2_Well10A
version: 1
owned by: myers
member: BBa_K737031

iGEM 2019 Distribution Plate 2 Well 18J
type: Collection
persistent identity: iGEM 2019 Plate2 Well18J
display id: iGEM_2019_Plate2_Well18J
version: 1
owned by: myers
member: spacer

iGEM 2019 Distribution Plate 2 Well 18O
type: Collection
persistent identity: iGEM 2019 Plate2 Well18O
display id: iGEM_2019_Plate2_Well18O
version: 1
owned by: myers
member: BBa_K801093

iGEM 2019 Distribution Plate 2 Well 8C
type: Collection
persistent identity: iGEM 2019 Plate2 Well8C
display id: iGEM_2019_Plate2_Well8C
version: 1
owned by: myers
member: BBa_K844007

iGEM 2019 Distribution Plate 2 Well 14A
type: Collection
persistent identity: iGEM 2019 Plate2 Well14A
display id: iGEM_2019_Plate2_Well14A
version: 1
owned by: myers
member: BBa_J202009

iGEM 2019 Distribution Plate 2 Well 16L
type: Collection
persistent identity: iGEM 2019 Plate2 Well16L
display id: iGEM_2019_Plate2_Well16L
version: 1
owned by: myers
member: BBa_S03877

iGEM 2019 Distribution Plate 2 Well 7D
type: Collection
persistent identity: iGEM 2019 Plate2 Well7D
display id: iGEM_2019_Plate2_Well7D
version: 1
owned by: myers
member: BBa_S0107

iGEM 2019 Distribution Plate 2 Well 20P
type: Collection
persistent identity: iGEM 2019 Plate2 Well20P
display id: iGEM_2019_Plate2_Well20P
version: 1
owned by: myers
member: BBa_I13453

iGEM 2019 Distribution Plate 2 Well 4H
type: Collection
persistent identity: iGEM 2019 Plate2 Well4H
display id: iGEM_2019_Plate2_Well4H
version: 1
owned by: myers
member: cinR

iGEM 2019 Distribution Plate 2 Well 10K
type: Collection
persistent identity: iGEM 2019 Plate2 Well10K
display id: iGEM_2019_Plate2_Well10K
version: 1
owned by: myers
member: BBa_K747094

iGEM 2019 Distribution Plate 2 Well 7E
type: Collection
persistent identity: iGEM 2019 Plate2 Well7E
display id: iGEM_2019_Plate2_Well7E
version: 1
owned by: myers
member: Ara

BBa_K863006
type: ComponentDefinition
persistent identity: BBa K863006
display id: BBa_K863006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ecol laccase from E. coli
creator: Isabel Huber
created: 2012-09-17T11:00:00Z
modified: 2015-05-08T01:13:36Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1123_
m user id: 0
owner id: 12844
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: E.coli laccase ORF
mutable notes: test
mutable provenance: E. coli BL21 (DE3)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: TAA
sequence annotation: ATG
sequence: https://synbiohub.org/public/igem/BBa_K863006_sequence/1

TAA
type: SequenceAnnotation
persistent identity: annotation2184436
display id: annotation2184436
version: 1
top level: BBa_K863006
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K863006/annotation2184436/range2184436/1

https://synbiohub.org/public/igem/BBa_K863006/annotation2184436/range2184436/1
type: Range
persistent identity: range2184436
display id: range2184436
version: 1
direction: direction/forward
top level: BBa_K863006
start: 1549
end: 1551
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2184435
display id: annotation2184435
version: 1
top level: BBa_K863006
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K863006/annotation2184435/range2184435/1

https://synbiohub.org/public/igem/BBa_K863006/annotation2184435/range2184435/1
type: Range
persistent identity: range2184435
display id: range2184435
version: 1
direction: direction/forward
top level: BBa_K863006
start: 1
end: 3
orientation: inline

ArgBox16
type: ComponentDefinition
persistent identity: BBa K778006
display id: BBa_K778006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ArgR binding sites (including 16 binding sites)
creator: Yuka Yamazaki
created: 2012-09-22T11:00:00Z
modified: 2015-05-08T01:13:17Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1032_
m user id: 0
owner id: 13134
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: BBa_K778003 ??2
mutable notes: sukima
mutable provenance: biobrick part (BBa_K778003)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
component: BBa_K778003
sequence annotation: BBa_K778003
sequence: https://synbiohub.org/public/igem/BBa_K778006_sequence/1

BBa_K778003
type: Component
persistent identity: component2194872
display id: component2194872
version: 1
top level: ArgBox16
access: public
definition: https://synbiohub.org/public/igem/BBa_K778003/1

BBa_K778003
type: Component
persistent identity: component2194871
display id: component2194871
version: 1
top level: ArgBox16
access: public
definition: https://synbiohub.org/public/igem/BBa_K778003/1

BBa_K778003
type: SequenceAnnotation
persistent identity: annotation2194871
display id: annotation2194871
version: 1
top level: ArgBox16
location: https://synbiohub.org/public/igem/BBa_K778006/annotation2194871/range2194871/1
component: BBa_K778003

https://synbiohub.org/public/igem/BBa_K778006/annotation2194871/range2194871/1
type: Range
persistent identity: range2194871
display id: range2194871
version: 1
direction: direction/reverse
top level: ArgBox16
start: 1
end: 144
orientation: inline

BBa_K778003
type: SequenceAnnotation
persistent identity: annotation2194872
display id: annotation2194872
version: 1
top level: ArgBox16
location: https://synbiohub.org/public/igem/BBa_K778006/annotation2194872/range2194872/1
component: BBa_K778003

https://synbiohub.org/public/igem/BBa_K778006/annotation2194872/range2194872/1
type: Range
persistent identity: range2194872
display id: range2194872
version: 1
direction: direction/reverse
top level: ArgBox16
start: 153
end: 296
orientation: inline

BBa_K909006
type: ComponentDefinition
persistent identity: BBa K909006
display id: BBa_K909006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Full-length lacZ with RBS and Terminator
creator: Isaak Mueller
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:44Z
discontinued: false
dominant: false
group u list: _1174_
m user id: 0
owner id: 14050
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: .
mutable notes: .
mutable provenance: .
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_I732017
component: BBa_B0017
sequence annotation: BBa_B0017
sequence annotation: BBa_I732017
sequence: https://synbiohub.org/public/igem/BBa_K909006_sequence/1

BBa_I732017
type: Component
persistent identity: component2195194
display id: component2195194
version: 1
top level: BBa_K909006
access: public
definition: https://synbiohub.org/public/igem/BBa_I732017/1

BBa_B0017
type: Component
persistent identity: component2195199
display id: component2195199
version: 1
top level: BBa_K909006
access: public
definition: https://synbiohub.org/public/igem/BBa_B0017/1

BBa_B0017
type: SequenceAnnotation
persistent identity: annotation2195199
display id: annotation2195199
version: 1
top level: BBa_K909006
location: https://synbiohub.org/public/igem/BBa_K909006/annotation2195199/range2195199/1
component: BBa_B0017

https://synbiohub.org/public/igem/BBa_K909006/annotation2195199/range2195199/1
type: Range
persistent identity: range2195199
display id: range2195199
version: 1
direction: direction/reverse
top level: BBa_K909006
start: 3102
end: 3269
orientation: inline

BBa_I732017
type: SequenceAnnotation
persistent identity: annotation2195194
display id: annotation2195194
version: 1
top level: BBa_K909006
location: https://synbiohub.org/public/igem/BBa_K909006/annotation2195194/range2195194/1
component: BBa_I732017

https://synbiohub.org/public/igem/BBa_K909006/annotation2195194/range2195194/1
type: Range
persistent identity: range2195194
display id: range2195194
version: 1
direction: direction/reverse
top level: BBa_K909006
start: 1
end: 3093
orientation: inline

BBa_S03859
type: ComponentDefinition
persistent identity: BBa S03859
display id: BBa_S03859
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0015:I718016
creator: Eimad Shotar
created: 2007-10-25T11:00:00Z
modified: 2015-05-08T01:14:31Z
discontinued: false
dominant: true
group u list: _9_
m user id: 0
owner id: 1568
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_I718016
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_I718016
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_S03859_sequence/1

BBa_I718016
type: Component
persistent identity: component1956475
display id: component1956475
version: 1
top level: BBa_S03859
access: public
definition: https://synbiohub.org/public/igem/BBa_I718016/1

BBa_B0012
type: Component
persistent identity: component1956471
display id: component1956471
version: 1
top level: BBa_S03859
access: public
definition: BBa_B0012

BBa_B0010
type: Component
persistent identity: component1956469
display id: component1956469
version: 1
top level: BBa_S03859
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1956469
display id: annotation1956469
version: 1
top level: BBa_S03859
location: https://synbiohub.org/public/igem/BBa_S03859/annotation1956469/range1956469/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_S03859/annotation1956469/range1956469/1
type: Range
persistent identity: range1956469
display id: range1956469
version: 1
direction: direction/forward
top level: BBa_S03859
start: 1
end: 80
orientation: inline

BBa_I718016
type: SequenceAnnotation
persistent identity: annotation1956475
display id: annotation1956475
version: 1
top level: BBa_S03859
location: https://synbiohub.org/public/igem/BBa_S03859/annotation1956475/range1956475/1
component: BBa_I718016

https://synbiohub.org/public/igem/BBa_S03859/annotation1956475/range1956475/1
type: Range
persistent identity: range1956475
display id: range1956475
version: 1
direction: direction/reverse
top level: BBa_S03859
start: 138
end: 171
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1956471
display id: annotation1956471
version: 1
top level: BBa_S03859
location: https://synbiohub.org/public/igem/BBa_S03859/annotation1956471/range1956471/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_S03859/annotation1956471/range1956471/1
type: Range
persistent identity: range1956471
display id: range1956471
version: 1
direction: direction/forward
top level: BBa_S03859
start: 89
end: 129
orientation: inline

BBa_K861031
type: ComponentDefinition
persistent identity: BBa K861031
display id: BBa_K861031
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: FadJ, gene for fatty acid degradation from E.coli str. K12
creator: Kuanwei Sheng
created: 2012-09-05T11:00:00Z
modified: 2015-05-08T01:13:35Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1121_
m user id: 0
owner id: 12357
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: FadJ is the anaerobic counterpart of FadB. The protein is shown to have better degradation of medium and short length fatty acid together with FadI.
mutable notes: None
mutable provenance: E.coli str.K12 lab mutant DH5α
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K861031_sequence/1

BBa_K876073
type: ComponentDefinition
persistent identity: BBa K876073
display id: BBa_K876073
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pTetR-LasR-Term-Term-pLasR-LuxS-RBS-CFP-Term-Term-pLuxR-TetR
creator: Zakaraya Ashour, Sanket Shah
created: 2012-10-01T11:00:00Z
modified: 2015-05-08T01:13:39Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1138_
m user id: 0
owner id: 13835
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: pTetR-LasR-TT-pLasR-LuxS-RBS-CFP-TT-pLuxR-TetR
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K091109
component: BBa_E0422
component: BBa_R0062
component: BBa_R0079
component: BBa_K876057
component: BBa_C0040
sequence annotation: BBa_R0062
sequence annotation: BBa_K091109
sequence annotation: BBa_C0040
sequence annotation: BBa_K876057
sequence annotation: BBa_E0422
sequence annotation: BBa_R0079
sequence: https://synbiohub.org/public/igem/BBa_K876073_sequence/1

BBa_K091109
type: Component
persistent identity: component2298671
display id: component2298671
version: 1
top level: BBa_K876073
access: public
definition: https://synbiohub.org/public/igem/BBa_K091109/1

BBa_E0422
type: Component
persistent identity: component2298685
display id: component2298685
version: 1
top level: BBa_K876073
access: public
definition: BBa_E0422

BBa_R0062
type: Component
persistent identity: component2298687
display id: component2298687
version: 1
top level: BBa_K876073
access: public
definition: lux pR

BBa_R0079
type: Component
persistent identity: component2298670
display id: component2298670
version: 1
top level: BBa_K876073
access: public
definition: LasR+PAI

BBa_K876057
type: Component
persistent identity: component2298665
display id: component2298665
version: 1
top level: BBa_K876073
access: public
definition: BBa_K876057

BBa_C0040
type: Component
persistent identity: component2298694
display id: component2298694
version: 1
top level: BBa_K876073
access: public
definition: tetR

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation2298687
display id: annotation2298687
version: 1
top level: BBa_K876073
location: https://synbiohub.org/public/igem/BBa_K876073/annotation2298687/range2298687/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_K876073/annotation2298687/range2298687/1
type: Range
persistent identity: range2298687
display id: range2298687
version: 1
direction: direction/forward
top level: BBa_K876073
start: 2599
end: 2653
orientation: inline

BBa_K091109
type: SequenceAnnotation
persistent identity: annotation2298671
display id: annotation2298671
version: 1
top level: BBa_K876073
location: https://synbiohub.org/public/igem/BBa_K876073/annotation2298671/range2298671/1
component: BBa_K091109

https://synbiohub.org/public/igem/BBa_K876073/annotation2298671/range2298671/1
type: Range
persistent identity: range2298671
display id: range2298671
version: 1
direction: direction/reverse
top level: BBa_K876073
start: 1150
end: 1665
orientation: inline

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation2298694
display id: annotation2298694
version: 1
top level: BBa_K876073
location: https://synbiohub.org/public/igem/BBa_K876073/annotation2298694/range2298694/1
component: BBa_C0040

https://synbiohub.org/public/igem/BBa_K876073/annotation2298694/range2298694/1
type: Range
persistent identity: range2298694
display id: range2298694
version: 1
direction: direction/reverse
top level: BBa_K876073
start: 2660
end: 3344
orientation: inline

BBa_K876057
type: SequenceAnnotation
persistent identity: annotation2298665
display id: annotation2298665
version: 1
top level: BBa_K876073
location: https://synbiohub.org/public/igem/BBa_K876073/annotation2298665/range2298665/1
component: BBa_K876057

https://synbiohub.org/public/igem/BBa_K876073/annotation2298665/range2298665/1
type: Range
persistent identity: range2298665
display id: range2298665
version: 1
direction: direction/reverse
top level: BBa_K876073
start: 1
end: 978
orientation: inline

BBa_E0422
type: SequenceAnnotation
persistent identity: annotation2298685
display id: annotation2298685
version: 1
top level: BBa_K876073
location: https://synbiohub.org/public/igem/BBa_K876073/annotation2298685/range2298685/1
component: BBa_E0422

https://synbiohub.org/public/igem/BBa_K876073/annotation2298685/range2298685/1
type: Range
persistent identity: range2298685
display id: range2298685
version: 1
direction: direction/reverse
top level: BBa_K876073
start: 1674
end: 2590
orientation: inline

BBa_R0079
type: SequenceAnnotation
persistent identity: annotation2298670
display id: annotation2298670
version: 1
top level: BBa_K876073
location: https://synbiohub.org/public/igem/BBa_K876073/annotation2298670/range2298670/1
component: BBa_R0079

https://synbiohub.org/public/igem/BBa_K876073/annotation2298670/range2298670/1
type: Range
persistent identity: range2298670
display id: range2298670
version: 1
direction: direction/reverse
top level: BBa_K876073
start: 987
end: 1143
orientation: inline

BBa_I13001
type: ComponentDefinition
persistent identity: BBa I13001
display id: BBa_I13001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS B0030 w/ YFP (-LVA) TT
creator: Jay Blumling, Debra Lin, Madeleine Sheldon-dante, Fred Tan, MIT SMUG
created: 2004-06-27T11:00:00Z
modified: 2015-08-31T04:07:32Z
discontinued: false
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 101
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: B0030. E0030. B0015
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0010
component: BBa_B0012
component: BBa_E0030
component: BBa_B0030
sequence annotation: BBa_E0030
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_B0030
sequence: https://synbiohub.org/public/igem/BBa_I13001_sequence/1

BBa_B0010
type: Component
persistent identity: component943436
display id: component943436
version: 1
top level: BBa_I13001
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component943446
display id: component943446
version: 1
top level: BBa_I13001
access: public
definition: BBa_B0012

BBa_E0030
type: Component
persistent identity: component943431
display id: component943431
version: 1
top level: BBa_I13001
access: public
definition: https://synbiohub.org/public/igem/BBa_E0030/1

BBa_B0030
type: Component
persistent identity: component943428
display id: component943428
version: 1
top level: BBa_I13001
access: public
definition: BBa_B0030

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation943431
display id: annotation943431
version: 1
top level: BBa_I13001
location: https://synbiohub.org/public/igem/BBa_I13001/annotation943431/range943431/1
component: BBa_E0030

https://synbiohub.org/public/igem/BBa_I13001/annotation943431/range943431/1
type: Range
persistent identity: range943431
display id: range943431
version: 1
direction: direction/forward
top level: BBa_I13001
start: 22
end: 744
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation943436
display id: annotation943436
version: 1
top level: BBa_I13001
location: https://synbiohub.org/public/igem/BBa_I13001/annotation943436/range943436/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13001/annotation943436/range943436/1
type: Range
persistent identity: range943436
display id: range943436
version: 1
direction: direction/forward
top level: BBa_I13001
start: 753
end: 832
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation943446
display id: annotation943446
version: 1
top level: BBa_I13001
location: https://synbiohub.org/public/igem/BBa_I13001/annotation943446/range943446/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13001/annotation943446/range943446/1
type: Range
persistent identity: range943446
display id: range943446
version: 1
direction: direction/forward
top level: BBa_I13001
start: 841
end: 881
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation943428
display id: annotation943428
version: 1
top level: BBa_I13001
location: https://synbiohub.org/public/igem/BBa_I13001/annotation943428/range943428/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_I13001/annotation943428/range943428/1
type: Range
persistent identity: range943428
display id: range943428
version: 1
direction: direction/forward
top level: BBa_I13001
start: 1
end: 15
orientation: inline

GFP genera
type: ComponentDefinition
persistent identity: BBa E0840
display id: BBa_E0840
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP generator
creator: Jennifer Braff
created: 2004-10-17T11:00:00Z
modified: 2015-08-31T04:07:26Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 61
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: B0030.E0040.B0015
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0030
component: BBa_E0040
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_E0040
sequence annotation: BBa_B0030
sequence: https://synbiohub.org/public/igem/BBa_E0840_sequence/1

BBa_B0030
type: Component
persistent identity: component1249239
display id: component1249239
version: 1
top level: GFP genera
access: public
definition: BBa_B0030

BBa_E0040
type: Component
persistent identity: component1249242
display id: component1249242
version: 1
top level: GFP genera
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_B0012
type: Component
persistent identity: component1249257
display id: component1249257
version: 1
top level: GFP genera
access: public
definition: BBa_B0012

BBa_B0010
type: Component
persistent identity: component1249247
display id: component1249247
version: 1
top level: GFP genera
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1249257
display id: annotation1249257
version: 1
top level: GFP genera
location: https://synbiohub.org/public/igem/BBa_E0840/annotation1249257/range1249257/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_E0840/annotation1249257/range1249257/1
type: Range
persistent identity: range1249257
display id: range1249257
version: 1
direction: direction/forward
top level: GFP genera
start: 838
end: 878
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1249247
display id: annotation1249247
version: 1
top level: GFP genera
location: https://synbiohub.org/public/igem/BBa_E0840/annotation1249247/range1249247/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_E0840/annotation1249247/range1249247/1
type: Range
persistent identity: range1249247
display id: range1249247
version: 1
direction: direction/forward
top level: GFP genera
start: 750
end: 829
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1249242
display id: annotation1249242
version: 1
top level: GFP genera
location: https://synbiohub.org/public/igem/BBa_E0840/annotation1249242/range1249242/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_E0840/annotation1249242/range1249242/1
type: Range
persistent identity: range1249242
display id: range1249242
version: 1
direction: direction/forward
top level: GFP genera
start: 22
end: 741
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1249239
display id: annotation1249239
version: 1
top level: GFP genera
location: https://synbiohub.org/public/igem/BBa_E0840/annotation1249239/range1249239/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_E0840/annotation1249239/range1249239/1
type: Range
persistent identity: range1249239
display id: range1249239
version: 1
direction: direction/forward
top level: GFP genera
start: 1
end: 15
orientation: inline

mRFP1
type: ComponentDefinition
persistent identity: BBa I13502
display id: BBa_I13502
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Screening plasmid intermediate
creator: jkm
created: 2005-05-30T11:00:00Z
modified: 2015-08-31T04:07:34Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Built by Josh as an intermediate in screening plasmid construction.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_B0034
component: BBa_E1010
sequence annotation: BBa_B0034
sequence annotation: BBa_E1010
sequence: https://synbiohub.org/public/igem/BBa_I13502_sequence/1

BBa_B0034
type: Component
persistent identity: component1505038
display id: component1505038
version: 1
top level: mRFP1
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_E1010
type: Component
persistent identity: component1505045
display id: component1505045
version: 1
top level: mRFP1
access: public
definition: https://synbiohub.org/public/igem/BBa_E1010/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1505038
display id: annotation1505038
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_I13502/annotation1505038/range1505038/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13502/annotation1505038/range1505038/1
type: Range
persistent identity: range1505038
display id: range1505038
version: 1
direction: direction/forward
top level: mRFP1
start: 1
end: 12
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation1505045
display id: annotation1505045
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_I13502/annotation1505045/range1505045/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_I13502/annotation1505045/range1505045/1
type: Range
persistent identity: range1505045
display id: range1505045
version: 1
direction: direction/forward
top level: mRFP1
start: 19
end: 699
orientation: inline

BBa_I732903
type: ComponentDefinition
persistent identity: BBa I732903
display id: BBa_I732903
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: R0011 I732020
creator: Zhan Jian
created: 2007-10-16T11:00:00Z
modified: 2015-08-31T04:08:00Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_I732006
component: BBa_B0012
component: BBa_B0034
component: BBa_R0011
component: BBa_B0010
sequence annotation: BBa_R0011
sequence annotation: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_B0010
sequence annotation: BBa_I732006
sequence: https://synbiohub.org/public/igem/BBa_I732903_sequence/1

BBa_I732006
type: Component
persistent identity: component1948580
display id: component1948580
version: 1
top level: BBa_I732903
access: public
definition: https://synbiohub.org/public/igem/BBa_I732006/1

BBa_B0012
type: Component
persistent identity: component1948583
display id: component1948583
version: 1
top level: BBa_I732903
access: public
definition: BBa_B0012

BBa_B0034
type: Component
persistent identity: component1948576
display id: component1948576
version: 1
top level: BBa_I732903
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0011
type: Component
persistent identity: component1948570
display id: component1948570
version: 1
top level: BBa_I732903
access: public
definition: lacI+pL

BBa_B0010
type: Component
persistent identity: component1948581
display id: component1948581
version: 1
top level: BBa_I732903
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation1948570
display id: annotation1948570
version: 1
top level: BBa_I732903
location: https://synbiohub.org/public/igem/BBa_I732903/annotation1948570/range1948570/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_I732903/annotation1948570/range1948570/1
type: Range
persistent identity: range1948570
display id: range1948570
version: 1
direction: direction/forward
top level: BBa_I732903
start: 1
end: 54
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1948583
display id: annotation1948583
version: 1
top level: BBa_I732903
location: https://synbiohub.org/public/igem/BBa_I732903/annotation1948583/range1948583/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I732903/annotation1948583/range1948583/1
type: Range
persistent identity: range1948583
display id: range1948583
version: 1
direction: direction/forward
top level: BBa_I732903
start: 412
end: 452
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1948576
display id: annotation1948576
version: 1
top level: BBa_I732903
location: https://synbiohub.org/public/igem/BBa_I732903/annotation1948576/range1948576/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I732903/annotation1948576/range1948576/1
type: Range
persistent identity: range1948576
display id: range1948576
version: 1
direction: direction/reverse
top level: BBa_I732903
start: 64
end: 75
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1948581
display id: annotation1948581
version: 1
top level: BBa_I732903
location: https://synbiohub.org/public/igem/BBa_I732903/annotation1948581/range1948581/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I732903/annotation1948581/range1948581/1
type: Range
persistent identity: range1948581
display id: range1948581
version: 1
direction: direction/forward
top level: BBa_I732903
start: 324
end: 403
orientation: inline

BBa_I732006
type: SequenceAnnotation
persistent identity: annotation1948580
display id: annotation1948580
version: 1
top level: BBa_I732903
location: https://synbiohub.org/public/igem/BBa_I732903/annotation1948580/range1948580/1
component: BBa_I732006

https://synbiohub.org/public/igem/BBa_I732903/annotation1948580/range1948580/1
type: Range
persistent identity: range1948580
display id: range1948580
version: 1
direction: direction/reverse
top level: BBa_I732903
start: 82
end: 315
orientation: inline

BBa_I732081
type: ComponentDefinition
persistent identity: BBa I732081
display id: BBa_I732081
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tet repressor (RBS+ LVA- TERM-)
creator: Zhan Jian
created: 2007-08-02T11:00:00Z
modified: 2015-08-31T04:07:57Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_B0034
component: BBa_I732080
sequence annotation: BBa_B0034
sequence annotation: BBa_I732080
sequence: https://synbiohub.org/public/igem/BBa_I732081_sequence/1

BBa_B0034
type: Component
persistent identity: component1940758
display id: component1940758
version: 1
top level: BBa_I732081
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_I732080
type: Component
persistent identity: component1940760
display id: component1940760
version: 1
top level: BBa_I732081
access: public
definition: https://synbiohub.org/public/igem/BBa_I732080/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1940758
display id: annotation1940758
version: 1
top level: BBa_I732081
location: https://synbiohub.org/public/igem/BBa_I732081/annotation1940758/range1940758/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I732081/annotation1940758/range1940758/1
type: Range
persistent identity: range1940758
display id: range1940758
version: 1
direction: direction/reverse
top level: BBa_I732081
start: 1
end: 12
orientation: inline

BBa_I732080
type: SequenceAnnotation
persistent identity: annotation1940760
display id: annotation1940760
version: 1
top level: BBa_I732081
location: https://synbiohub.org/public/igem/BBa_I732081/annotation1940760/range1940760/1
component: BBa_I732080

https://synbiohub.org/public/igem/BBa_I732081/annotation1940760/range1940760/1
type: Range
persistent identity: range1940760
display id: range1940760
version: 1
direction: direction/reverse
top level: BBa_I732081
start: 19
end: 645
orientation: inline

BBa_I6040
type: ComponentDefinition
persistent identity: BBa I6040
display id: BBa_I6040
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter O_H Test R0053.E0430
creator: Caitlin Conboy and Jennifer Braff
created: 2004-03-16T12:00:00Z
modified: 2015-08-31T04:07:43Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_R0053
component: BBa_B0012
component: BBa_B0010
component: BBa_B0034
component: BBa_E0030
sequence annotation: BBa_R0053
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_E0030
sequence: https://synbiohub.org/public/igem/BBa_I6040_sequence/1

BBa_R0053
type: Component
persistent identity: component948497
display id: component948497
version: 1
top level: BBa_I6040
access: public
definition: https://synbiohub.org/public/igem/BBa_R0053/1

BBa_B0012
type: Component
persistent identity: component948524
display id: component948524
version: 1
top level: BBa_I6040
access: public
definition: BBa_B0012

BBa_B0010
type: Component
persistent identity: component948514
display id: component948514
version: 1
top level: BBa_I6040
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component948507
display id: component948507
version: 1
top level: BBa_I6040
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_E0030
type: Component
persistent identity: component948509
display id: component948509
version: 1
top level: BBa_I6040
access: public
definition: https://synbiohub.org/public/igem/BBa_E0030/1

BBa_R0053
type: SequenceAnnotation
persistent identity: annotation948497
display id: annotation948497
version: 1
top level: BBa_I6040
location: https://synbiohub.org/public/igem/BBa_I6040/annotation948497/range948497/1
component: BBa_R0053

https://synbiohub.org/public/igem/BBa_I6040/annotation948497/range948497/1
type: Range
persistent identity: range948497
display id: range948497
version: 1
direction: direction/forward
top level: BBa_I6040
start: 1
end: 54
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation948514
display id: annotation948514
version: 1
top level: BBa_I6040
location: https://synbiohub.org/public/igem/BBa_I6040/annotation948514/range948514/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I6040/annotation948514/range948514/1
type: Range
persistent identity: range948514
display id: range948514
version: 1
direction: direction/forward
top level: BBa_I6040
start: 812
end: 891
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation948524
display id: annotation948524
version: 1
top level: BBa_I6040
location: https://synbiohub.org/public/igem/BBa_I6040/annotation948524/range948524/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I6040/annotation948524/range948524/1
type: Range
persistent identity: range948524
display id: range948524
version: 1
direction: direction/forward
top level: BBa_I6040
start: 900
end: 940
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation948507
display id: annotation948507
version: 1
top level: BBa_I6040
location: https://synbiohub.org/public/igem/BBa_I6040/annotation948507/range948507/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I6040/annotation948507/range948507/1
type: Range
persistent identity: range948507
display id: range948507
version: 1
direction: direction/forward
top level: BBa_I6040
start: 63
end: 74
orientation: inline

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation948509
display id: annotation948509
version: 1
top level: BBa_I6040
location: https://synbiohub.org/public/igem/BBa_I6040/annotation948509/range948509/1
component: BBa_E0030

https://synbiohub.org/public/igem/BBa_I6040/annotation948509/range948509/1
type: Range
persistent identity: range948509
display id: range948509
version: 1
direction: direction/forward
top level: BBa_I6040
start: 81
end: 803
orientation: inline

BBa_I732801
type: ComponentDefinition
persistent identity: BBa I732801
display id: BBa_I732801
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LRLa (RBS+, TERM-)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:59Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_B0034
component: BBa_I732101
sequence annotation: BBa_I732101
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_I732801_sequence/1

BBa_B0034
type: Component
persistent identity: component1946165
display id: component1946165
version: 1
top level: BBa_I732801
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_I732101
type: Component
persistent identity: component1946166
display id: component1946166
version: 1
top level: BBa_I732801
access: public
definition: LRLa

BBa_I732101
type: SequenceAnnotation
persistent identity: annotation1946166
display id: annotation1946166
version: 1
top level: BBa_I732801
location: https://synbiohub.org/public/igem/BBa_I732801/annotation1946166/range1946166/1
component: BBa_I732101

https://synbiohub.org/public/igem/BBa_I732801/annotation1946166/range1946166/1
type: Range
persistent identity: range1946166
display id: range1946166
version: 1
direction: direction/reverse
top level: BBa_I732801
start: 19
end: 1104
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1946165
display id: annotation1946165
version: 1
top level: BBa_I732801
location: https://synbiohub.org/public/igem/BBa_I732801/annotation1946165/range1946165/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I732801/annotation1946165/range1946165/1
type: Range
persistent identity: range1946165
display id: range1946165
version: 1
direction: direction/reverse
top level: BBa_I732801
start: 1
end: 12
orientation: inline

BBa_K844000
type: ComponentDefinition
persistent identity: BBa K844000
display id: BBa_K844000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 10x-Histidine (10x-His) Tag with double stop codon (TAATAA)
creator: Kathleen Miller
created: 2012-10-01T11:00:00Z
modified: 2015-05-08T01:13:33Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1104_
m user id: 0
owner id: 9404
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: 10x-Histidine tag with double stop codon TAATAA to allow for better extraction of tagged products and protein termination in a single part.
mutable notes: none
mutable provenance: Constructed through oligonucleotide annealing
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence annotation: Stop
sequence annotation: 10x-Histidine Tag
sequence: https://synbiohub.org/public/igem/BBa_K844000_sequence/1

Stop
type: SequenceAnnotation
persistent identity: annotation2206608
display id: annotation2206608
version: 1
top level: BBa_K844000
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K844000/annotation2206608/range2206608/1

https://synbiohub.org/public/igem/BBa_K844000/annotation2206608/range2206608/1
type: Range
persistent identity: range2206608
display id: range2206608
version: 1
direction: direction/forward
top level: BBa_K844000
start: 31
end: 33
orientation: inline

10x-Histidine Tag
type: SequenceAnnotation
persistent identity: annotation2206607
display id: annotation2206607
version: 1
top level: BBa_K844000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K844000/annotation2206607/range2206607/1

https://synbiohub.org/public/igem/BBa_K844000/annotation2206607/range2206607/1
type: Range
persistent identity: range2206607
display id: range2206607
version: 1
direction: direction/forward
top level: BBa_K844000
start: 1
end: 30
orientation: inline

Stop
type: SequenceAnnotation
persistent identity: annotation2206609
display id: annotation2206609
version: 1
top level: BBa_K844000
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K844000/annotation2206609/range2206609/1

https://synbiohub.org/public/igem/BBa_K844000/annotation2206609/range2206609/1
type: Range
persistent identity: range2206609
display id: range2206609
version: 1
direction: direction/forward
top level: BBa_K844000
start: 34
end: 36
orientation: inline

BBa_S01002
type: ComponentDefinition
persistent identity: BBa S01002
display id: BBa_S01002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: R0071.E0422
created: 2005-06-10T11:00:00Z
modified: 2015-05-08T01:14:16Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 25
owning group id: 1
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Intermediate part from Jen 1 Tube 46
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_B0010
component: BBa_E0022
component: BBa_R0071
component: BBa_B0034
component: BBa_B0012
sequence annotation: BBa_E0022
sequence annotation: BBa_B0012
sequence annotation: BBa_R0071
sequence annotation: BBa_B0010
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_S01002_sequence/1

BBa_B0010
type: Component
persistent identity: component1511051
display id: component1511051
version: 1
top level: BBa_S01002
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_E0022
type: Component
persistent identity: component1511043
display id: component1511043
version: 1
top level: BBa_S01002
access: public
definition: ECFP

BBa_R0071
type: Component
persistent identity: component1511026
display id: component1511026
version: 1
top level: BBa_S01002
access: public
definition: RhlR+C4

BBa_B0034
type: Component
persistent identity: component1511031
display id: component1511031
version: 1
top level: BBa_S01002
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: Component
persistent identity: component1511061
display id: component1511061
version: 1
top level: BBa_S01002
access: public
definition: BBa_B0012

BBa_E0022
type: SequenceAnnotation
persistent identity: annotation1511043
display id: annotation1511043
version: 1
top level: BBa_S01002
location: https://synbiohub.org/public/igem/BBa_S01002/annotation1511043/range1511043/1
component: BBa_E0022

https://synbiohub.org/public/igem/BBa_S01002/annotation1511043/range1511043/1
type: Range
persistent identity: range1511043
display id: range1511043
version: 1
direction: direction/forward
top level: BBa_S01002
start: 80
end: 841
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1511061
display id: annotation1511061
version: 1
top level: BBa_S01002
location: https://synbiohub.org/public/igem/BBa_S01002/annotation1511061/range1511061/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_S01002/annotation1511061/range1511061/1
type: Range
persistent identity: range1511061
display id: range1511061
version: 1
direction: direction/forward
top level: BBa_S01002
start: 938
end: 978
orientation: inline

BBa_R0071
type: SequenceAnnotation
persistent identity: annotation1511026
display id: annotation1511026
version: 1
top level: BBa_S01002
location: https://synbiohub.org/public/igem/BBa_S01002/annotation1511026/range1511026/1
component: BBa_R0071

https://synbiohub.org/public/igem/BBa_S01002/annotation1511026/range1511026/1
type: Range
persistent identity: range1511026
display id: range1511026
version: 1
direction: direction/forward
top level: BBa_S01002
start: 1
end: 53
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1511051
display id: annotation1511051
version: 1
top level: BBa_S01002
location: https://synbiohub.org/public/igem/BBa_S01002/annotation1511051/range1511051/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_S01002/annotation1511051/range1511051/1
type: Range
persistent identity: range1511051
display id: range1511051
version: 1
direction: direction/forward
top level: BBa_S01002
start: 850
end: 929
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1511031
display id: annotation1511031
version: 1
top level: BBa_S01002
location: https://synbiohub.org/public/igem/BBa_S01002/annotation1511031/range1511031/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_S01002/annotation1511031/range1511031/1
type: Range
persistent identity: range1511031
display id: range1511031
version: 1
direction: direction/forward
top level: BBa_S01002
start: 62
end: 73
orientation: inline

BBa_K808032
type: ComponentDefinition
persistent identity: BBa K808032
display id: BBa_K808032
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Arabinose regulated RBS-PhoA-His6tag-pNBEst13-Myctag-EstA
creator: Marie Burghard, Jascha Diemer, Philipp Rottmann, Rene Sahm, Arne Wehling
created: 2012-09-03T11:00:00Z
modified: 2015-05-08T01:13:26Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1065_
m user id: 0
owner id: 12529
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This a a composite of 2 different BioBricks by an standard assembly, forming a scar in between. It contains the following units: AraC-AraO2-AraO1-AraPromo(PBAD)- RBS-PhoA-His6tag-pNBEst13-Myctag-EstA which is similiar to BBa_K808000 & BBa_K808030 Its a regulatory system, being induced by adding 1 to 2% of mw% L-Arabinose. It is made for regulating the cell surface Expression of our PET cleaving enzyme pNB-Est13, which is anchored c-terminal to EstA.
mutable notes: We were not able to add this part as an composite due to missing links and unedited registry pages. Necessary informations are featured in the sequence.
mutable provenance: assembled GENEART synthesized product(BBa_K808030)and cloned regulatory unit (BBa_K808000), deriving from an expression system (pBAD-vector)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: BBa_K808028
sequence annotation: BBa_B0029
sequence annotation: scar
sequence annotation: AraC
sequence annotation: ara O1
sequence annotation: myctag
sequence annotation: BBa_K808026
sequence annotation: BBa_K808027
sequence annotation: stop
sequence annotation: ara promotor
sequence annotation: ara O2
sequence annotation: 6histag
sequence: https://synbiohub.org/public/igem/BBa_K808032_sequence/1

BBa_K808028
type: SequenceAnnotation
persistent identity: annotation2183171
display id: annotation2183171
version: 1
top level: BBa_K808032
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K808032/annotation2183171/range2183171/1

https://synbiohub.org/public/igem/BBa_K808032/annotation2183171/range2183171/1
type: Range
persistent identity: range2183171
display id: range2183171
version: 1
direction: direction/forward
top level: BBa_K808032
start: 1241
end: 1303
orientation: inline

BBa_B0029
type: SequenceAnnotation
persistent identity: annotation2183169
display id: annotation2183169
version: 1
top level: BBa_K808032
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K808032/annotation2183169/range2183169/1

https://synbiohub.org/public/igem/BBa_K808032/annotation2183169/range2183169/1
type: Range
persistent identity: range2183169
display id: range2183169
version: 1
direction: direction/forward
top level: BBa_K808032
start: 1220
end: 1234
orientation: inline

scar
type: SequenceAnnotation
persistent identity: annotation2183168
display id: annotation2183168
version: 1
top level: BBa_K808032
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K808032/annotation2183168/range2183168/1

https://synbiohub.org/public/igem/BBa_K808032/annotation2183168/range2183168/1
type: Range
persistent identity: range2183168
display id: range2183168
version: 1
direction: direction/forward
top level: BBa_K808032
start: 1211
end: 1218
orientation: inline

AraC
type: SequenceAnnotation
persistent identity: annotation2183164
display id: annotation2183164
version: 1
top level: BBa_K808032
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K808032/annotation2183164/range2183164/1

https://synbiohub.org/public/igem/BBa_K808032/annotation2183164/range2183164/1
type: Range
persistent identity: range2183164
display id: range2183164
version: 1
direction: direction/forward
top level: BBa_K808032
start: 1
end: 879
orientation: inline

ara O1
type: SequenceAnnotation
persistent identity: annotation2183166
display id: annotation2183166
version: 1
top level: BBa_K808032
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K808032/annotation2183166/range2183166/1

https://synbiohub.org/public/igem/BBa_K808032/annotation2183166/range2183166/1
type: Range
persistent identity: range2183166
display id: range2183166
version: 1
direction: direction/forward
top level: BBa_K808032
start: 1066
end: 1077
orientation: inline

myctag
type: SequenceAnnotation
persistent identity: annotation2183173
display id: annotation2183173
version: 1
top level: BBa_K808032
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K808032/annotation2183173/range2183173/1

https://synbiohub.org/public/igem/BBa_K808032/annotation2183173/range2183173/1
type: Range
persistent identity: range2183173
display id: range2183173
version: 1
direction: direction/forward
top level: BBa_K808032
start: 2792
end: 2821
orientation: inline

BBa_K808026
type: SequenceAnnotation
persistent identity: annotation2183172
display id: annotation2183172
version: 1
top level: BBa_K808032
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K808032/annotation2183172/range2183172/1

https://synbiohub.org/public/igem/BBa_K808032/annotation2183172/range2183172/1
type: Range
persistent identity: range2183172
display id: range2183172
version: 1
direction: direction/forward
top level: BBa_K808032
start: 1322
end: 2791
orientation: inline

BBa_K808027
type: SequenceAnnotation
persistent identity: annotation2183174
display id: annotation2183174
version: 1
top level: BBa_K808032
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K808032/annotation2183174/range2183174/1

https://synbiohub.org/public/igem/BBa_K808032/annotation2183174/range2183174/1
type: Range
persistent identity: range2183174
display id: range2183174
version: 1
direction: direction/forward
top level: BBa_K808032
start: 2822
end: 4255
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2183175
display id: annotation2183175
version: 1
top level: BBa_K808032
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K808032/annotation2183175/range2183175/1

https://synbiohub.org/public/igem/BBa_K808032/annotation2183175/range2183175/1
type: Range
persistent identity: range2183175
display id: range2183175
version: 1
direction: direction/forward
top level: BBa_K808032
start: 4256
end: 4258
orientation: inline

ara promotor
type: SequenceAnnotation
persistent identity: annotation2183167
display id: annotation2183167
version: 1
top level: BBa_K808032
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K808032/annotation2183167/range2183167/1

https://synbiohub.org/public/igem/BBa_K808032/annotation2183167/range2183167/1
type: Range
persistent identity: range2183167
display id: range2183167
version: 1
direction: direction/forward
top level: BBa_K808032
start: 1153
end: 1181
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2183176
display id: annotation2183176
version: 1
top level: BBa_K808032
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K808032/annotation2183176/range2183176/1

https://synbiohub.org/public/igem/BBa_K808032/annotation2183176/range2183176/1
type: Range
persistent identity: range2183176
display id: range2183176
version: 1
direction: direction/forward
top level: BBa_K808032
start: 4259
end: 4261
orientation: inline

ara O2
type: SequenceAnnotation
persistent identity: annotation2183165
display id: annotation2183165
version: 1
top level: BBa_K808032
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K808032/annotation2183165/range2183165/1

https://synbiohub.org/public/igem/BBa_K808032/annotation2183165/range2183165/1
type: Range
persistent identity: range2183165
display id: range2183165
version: 1
direction: direction/forward
top level: BBa_K808032
start: 909
end: 924
orientation: inline

6histag
type: SequenceAnnotation
persistent identity: annotation2183170
display id: annotation2183170
version: 1
top level: BBa_K808032
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K808032/annotation2183170/range2183170/1

https://synbiohub.org/public/igem/BBa_K808032/annotation2183170/range2183170/1
type: Range
persistent identity: range2183170
display id: range2183170
version: 1
direction: direction/forward
top level: BBa_K808032
start: 1304
end: 1321
orientation: inline

BBa_K769022
type: ComponentDefinition
persistent identity: BBa K769022
display id: BBa_K769022
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: lux operon(K769011) + GFP(I13522)
creator: Tomomi YOKOYAMA
created: 2012-09-18T11:00:00Z
modified: 2015-05-08T01:13:14Z
discontinued: false
dominant: false
group u list: _1021_
m user id: 0
owner id: 14294
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: lux operon(K769011) + GFP(I13522)
mutable notes: amprify green light
mutable provenance: P.kishitanii
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K769011
component: BBa_I13522
sequence annotation: BBa_K769011
sequence annotation: BBa_I13522
sequence: https://synbiohub.org/public/igem/BBa_K769022_sequence/1

BBa_K769011
type: Component
persistent identity: component2186513
display id: component2186513
version: 1
top level: BBa_K769022
access: public
definition: https://synbiohub.org/public/igem/BBa_K769011/1

BBa_I13522
type: Component
persistent identity: component2186528
display id: component2186528
version: 1
top level: BBa_K769022
access: public
definition: https://synbiohub.org/public/igem/BBa_I13522/1

BBa_K769011
type: SequenceAnnotation
persistent identity: annotation2186513
display id: annotation2186513
version: 1
top level: BBa_K769022
location: https://synbiohub.org/public/igem/BBa_K769022/annotation2186513/range2186513/1
component: BBa_K769011

https://synbiohub.org/public/igem/BBa_K769022/annotation2186513/range2186513/1
type: Range
persistent identity: range2186513
display id: range2186513
version: 1
direction: direction/reverse
top level: BBa_K769022
start: 1
end: 8531
orientation: inline

BBa_I13522
type: SequenceAnnotation
persistent identity: annotation2186528
display id: annotation2186528
version: 1
top level: BBa_K769022
location: https://synbiohub.org/public/igem/BBa_K769022/annotation2186528/range2186528/1
component: BBa_I13522

https://synbiohub.org/public/igem/BBa_K769022/annotation2186528/range2186528/1
type: Range
persistent identity: range2186528
display id: range2186528
version: 1
direction: direction/reverse
top level: BBa_K769022
start: 8540
end: 9476
orientation: inline

BBa_I13025
type: ComponentDefinition
persistent identity: BBa I13025
display id: BBa_I13025
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: mnt QPI Intermediate
creator: jenmitch
created: 2004-07-14T11:00:00Z
modified: 2015-08-31T04:07:32Z
discontinued: false
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 159
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_C0072
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_C0072
sequence: https://synbiohub.org/public/igem/BBa_I13025_sequence/1

BBa_C0072
type: Component
persistent identity: component939424
display id: component939424
version: 1
top level: BBa_I13025
access: public
definition: https://synbiohub.org/public/igem/BBa_C0072/1

BBa_B0034
type: Component
persistent identity: component939411
display id: component939411
version: 1
top level: BBa_I13025
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation939411
display id: annotation939411
version: 1
top level: BBa_I13025
location: https://synbiohub.org/public/igem/BBa_I13025/annotation939411/range939411/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13025/annotation939411/range939411/1
type: Range
persistent identity: range939411
display id: range939411
version: 1
direction: direction/forward
top level: BBa_I13025
start: 1
end: 12
orientation: inline

BBa_C0072
type: SequenceAnnotation
persistent identity: annotation939424
display id: annotation939424
version: 1
top level: BBa_I13025
location: https://synbiohub.org/public/igem/BBa_I13025/annotation939424/range939424/1
component: BBa_C0072

https://synbiohub.org/public/igem/BBa_I13025/annotation939424/range939424/1
type: Range
persistent identity: range939424
display id: range939424
version: 1
direction: direction/forward
top level: BBa_I13025
start: 19
end: 306
orientation: inline

BBa_B0013
type: ComponentDefinition
persistent identity: BBa B0013
display id: BBa_B0013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TE from coliphage T7 (+/-)
creator: Reshma Shetty
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Strong transcriptional terminator consisting of a 20 bp stem-loop that has been engineered to be bidirectional.
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Subsequently mutations were introduced to make the terminator bi-directional (i.e., AAAAAA insertion on 5' side of the stem loop). Additional mutations were introduced on the 3' side of the stem loop to increase the number of T's and eliminate any promoter -10 site that might be present to avoid initiation of transcription of whatever is downstream. Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 V01146 .
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0013
sequence annotation: polya
sequence annotation: T7 TE
sequence annotation: C
sequence annotation: ''
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0013_sequence/1

BBa_B0013
type: SequenceAnnotation
persistent identity: annotation7021
display id: annotation7021
version: 1
top level: BBa_B0013
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B0013/annotation7021/range7021/1

https://synbiohub.org/public/igem/BBa_B0013/annotation7021/range7021/1
type: Range
persistent identity: range7021
display id: range7021
version: 1
direction: direction/forward
top level: BBa_B0013
start: 1
end: 47
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1698
display id: annotation1698
version: 1
top level: BBa_B0013
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_B0013/annotation1698/range1698/1

https://synbiohub.org/public/igem/BBa_B0013/annotation1698/range1698/1
type: Range
persistent identity: range1698
display id: range1698
version: 1
direction: direction/forward
top level: BBa_B0013
start: 34
end: 47
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1691
display id: annotation1691
version: 1
top level: BBa_B0013
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_B0013/annotation1691/range1691/1

https://synbiohub.org/public/igem/BBa_B0013/annotation1691/range1691/1
type: Range
persistent identity: range1691
display id: range1691
version: 1
direction: direction/forward
top level: BBa_B0013
start: 14
end: 33
orientation: inline

C
type: SequenceAnnotation
persistent identity: annotation1696
display id: annotation1696
version: 1
top level: BBa_B0013
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_B0013/annotation1696/range1696/1

https://synbiohub.org/public/igem/BBa_B0013/annotation1696/range1696/1
type: Range
persistent identity: range1696
display id: range1696
version: 1
direction: direction/forward
top level: BBa_B0013
start: 10
end: 10
orientation: inline

''
type: SequenceAnnotation
persistent identity: annotation1697
display id: annotation1697
version: 1
top level: BBa_B0013
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_B0013/annotation1697/range1697/1

https://synbiohub.org/public/igem/BBa_B0013/annotation1697/range1697/1
type: Range
persistent identity: range1697
display id: range1697
version: 1
direction: direction/forward
top level: BBa_B0013
start: 1
end: 6
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1692
display id: annotation1692
version: 1
top level: BBa_B0013
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_B0013/annotation1692/range1692/1

https://synbiohub.org/public/igem/BBa_B0013/annotation1692/range1692/1
type: Range
persistent identity: range1692
display id: range1692
version: 1
direction: direction/forward
top level: BBa_B0013
start: 40
end: 40
orientation: inline

C
type: SequenceAnnotation
persistent identity: annotation1695
display id: annotation1695
version: 1
top level: BBa_B0013
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_B0013/annotation1695/range1695/1

https://synbiohub.org/public/igem/BBa_B0013/annotation1695/range1695/1
type: Range
persistent identity: range1695
display id: range1695
version: 1
direction: direction/forward
top level: BBa_B0013
start: 37
end: 37
orientation: inline

otsA
type: ComponentDefinition
persistent identity: BBa K847060
display id: BBa_K847060
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ''Escherichia coli'' osmoregulatory trehalose synthesis A (otsA)
creator: Rashmi Sharma, Vishesh Jain, Kendrick Wang
created: 2012-08-08T11:00:00Z
modified: 2015-05-08T01:13:34Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1107_
m user id: 0
owner id: 11092
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: tba
mutable notes: tba
mutable provenance: tba
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K847060_sequence/1

BBa_S03119
type: ComponentDefinition
persistent identity: BBa S03119
display id: BBa_S03119
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: --Specify Parts List--
creator: Randy Rettberg
created: 2004-04-19T11:00:00Z
modified: 2015-05-08T01:14:19Z
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Intermediate part from assembly 299
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_C0062
component: BBa_R0040
component: BBa_B0010
component: BBa_B0012
component: BBa_B0034
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_C0062
sequence annotation: BBa_R0040
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_S03119_sequence/1

BBa_C0062
type: Component
persistent identity: component955049
display id: component955049
version: 1
top level: BBa_S03119
access: public
definition: luxr

BBa_R0040
type: Component
persistent identity: component955026
display id: component955026
version: 1
top level: BBa_S03119
access: public
definition: p(tetR)

BBa_B0010
type: Component
persistent identity: component955057
display id: component955057
version: 1
top level: BBa_S03119
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component955067
display id: component955067
version: 1
top level: BBa_S03119
access: public
definition: BBa_B0012

BBa_B0034
type: Component
persistent identity: component955034
display id: component955034
version: 1
top level: BBa_S03119
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation955067
display id: annotation955067
version: 1
top level: BBa_S03119
location: https://synbiohub.org/public/igem/BBa_S03119/annotation955067/range955067/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_S03119/annotation955067/range955067/1
type: Range
persistent identity: range955067
display id: range955067
version: 1
direction: direction/forward
top level: BBa_S03119
start: 958
end: 998
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation955057
display id: annotation955057
version: 1
top level: BBa_S03119
location: https://synbiohub.org/public/igem/BBa_S03119/annotation955057/range955057/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_S03119/annotation955057/range955057/1
type: Range
persistent identity: range955057
display id: range955057
version: 1
direction: direction/forward
top level: BBa_S03119
start: 870
end: 949
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation955049
display id: annotation955049
version: 1
top level: BBa_S03119
location: https://synbiohub.org/public/igem/BBa_S03119/annotation955049/range955049/1
component: BBa_C0062

https://synbiohub.org/public/igem/BBa_S03119/annotation955049/range955049/1
type: Range
persistent identity: range955049
display id: range955049
version: 1
direction: direction/forward
top level: BBa_S03119
start: 81
end: 836
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation955026
display id: annotation955026
version: 1
top level: BBa_S03119
location: https://synbiohub.org/public/igem/BBa_S03119/annotation955026/range955026/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_S03119/annotation955026/range955026/1
type: Range
persistent identity: range955026
display id: range955026
version: 1
direction: direction/forward
top level: BBa_S03119
start: 1
end: 54
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation955034
display id: annotation955034
version: 1
top level: BBa_S03119
location: https://synbiohub.org/public/igem/BBa_S03119/annotation955034/range955034/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_S03119/annotation955034/range955034/1
type: Range
persistent identity: range955034
display id: range955034
version: 1
direction: direction/forward
top level: BBa_S03119
start: 63
end: 74
orientation: inline

BBa_B0033
type: ComponentDefinition
persistent identity: BBa B0033
display id: BBa_B0033
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS.4 (weaker) -- derivative of BBa_0030
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Weaker RBS based on Ron Weiss thesis. Strengths relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>.
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-3" in figure 4-14 of thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS-4\Weaker
sequence annotation: BBa_B0033
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0033_sequence/1

RBS-4\Weaker
type: SequenceAnnotation
persistent identity: annotation1713
display id: annotation1713
version: 1
top level: BBa_B0033
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_B0033/annotation1713/range1713/1

https://synbiohub.org/public/igem/BBa_B0033/annotation1713/range1713/1
type: Range
persistent identity: range1713
display id: range1713
version: 1
direction: direction/forward
top level: BBa_B0033
start: 1
end: 11
orientation: inline

BBa_B0033
type: SequenceAnnotation
persistent identity: annotation7028
display id: annotation7028
version: 1
top level: BBa_B0033
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B0033/annotation7028/range7028/1

https://synbiohub.org/public/igem/BBa_B0033/annotation7028/range7028/1
type: Range
persistent identity: range7028
display id: range7028
version: 1
direction: direction/forward
top level: BBa_B0033
start: 1
end: 11
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1714
display id: annotation1714
version: 1
top level: BBa_B0033
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_B0033/annotation1714/range1714/1

https://synbiohub.org/public/igem/BBa_B0033/annotation1714/range1714/1
type: Range
persistent identity: range1714
display id: range1714
version: 1
direction: direction/forward
top level: BBa_B0033
start: 7
end: 10
orientation: inline

BBa_B0032
type: ComponentDefinition
persistent identity: BBa B0032
display id: BBa_B0032
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS.3 (medium) -- derivative of BBa_0030
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). Contact info for this part: Brian Chow
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS-3\Weak
sequence annotation: RBS
sequence annotation: BBa_B0032
sequence: https://synbiohub.org/public/igem/BBa_B0032_sequence/1

RBS-3\Weak
type: SequenceAnnotation
persistent identity: annotation1709
display id: annotation1709
version: 1
top level: BBa_B0032
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1

https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
type: Range
persistent identity: range1709
display id: range1709
version: 1
direction: direction/forward
top level: BBa_B0032
start: 1
end: 13
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1710
display id: annotation1710
version: 1
top level: BBa_B0032
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1

https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
type: Range
persistent identity: range1710
display id: range1710
version: 1
direction: direction/forward
top level: BBa_B0032
start: 7
end: 10
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation7027
display id: annotation7027
version: 1
top level: BBa_B0032
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1

https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
type: Range
persistent identity: range7027
display id: range7027
version: 1
direction: direction/forward
top level: BBa_B0032
start: 1
end: 13
orientation: inline

BBa_P0151
type: ComponentDefinition
persistent identity: BBa P0151
display id: BBa_P0151
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PoPS -> cI (lambda) [S0165]
creator: Randy Rettberg
created: 2004-04-24T11:00:00Z
modified: 2015-05-08T01:14:10Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Protein generator converting TIPS to the protein cI from lambda. Used as the input section for Quad Part Inverters Q01150 and Q01151.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0012
component: BBa_B0010
component: BBa_C0051
component: BBa_B0031
sequence annotation: BBa_C0051
sequence annotation: BBa_B0012
sequence annotation: BBa_B0031
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_P0151_sequence/1

BBa_B0012
type: Component
persistent identity: component944529
display id: component944529
version: 1
top level: BBa_P0151
access: public
definition: BBa_B0012

BBa_B0010
type: Component
persistent identity: component944519
display id: component944519
version: 1
top level: BBa_P0151
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_C0051
type: Component
persistent identity: component944513
display id: component944513
version: 1
top level: BBa_P0151
access: public
definition: cI lam

BBa_B0031
type: Component
persistent identity: component944503
display id: component944503
version: 1
top level: BBa_P0151
access: public
definition: BBa_B0031

BBa_C0051
type: SequenceAnnotation
persistent identity: annotation944513
display id: annotation944513
version: 1
top level: BBa_P0151
location: https://synbiohub.org/public/igem/BBa_P0151/annotation944513/range944513/1
component: BBa_C0051

https://synbiohub.org/public/igem/BBa_P0151/annotation944513/range944513/1
type: Range
persistent identity: range944513
display id: range944513
version: 1
direction: direction/forward
top level: BBa_P0151
start: 21
end: 770
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation944529
display id: annotation944529
version: 1
top level: BBa_P0151
location: https://synbiohub.org/public/igem/BBa_P0151/annotation944529/range944529/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_P0151/annotation944529/range944529/1
type: Range
persistent identity: range944529
display id: range944529
version: 1
direction: direction/forward
top level: BBa_P0151
start: 892
end: 932
orientation: inline

BBa_B0031
type: SequenceAnnotation
persistent identity: annotation944503
display id: annotation944503
version: 1
top level: BBa_P0151
location: https://synbiohub.org/public/igem/BBa_P0151/annotation944503/range944503/1
component: BBa_B0031

https://synbiohub.org/public/igem/BBa_P0151/annotation944503/range944503/1
type: Range
persistent identity: range944503
display id: range944503
version: 1
direction: direction/forward
top level: BBa_P0151
start: 1
end: 14
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation944519
display id: annotation944519
version: 1
top level: BBa_P0151
location: https://synbiohub.org/public/igem/BBa_P0151/annotation944519/range944519/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_P0151/annotation944519/range944519/1
type: Range
persistent identity: range944519
display id: range944519
version: 1
direction: direction/forward
top level: BBa_P0151
start: 804
end: 883
orientation: inline

BBa_J202008
type: ComponentDefinition
persistent identity: BBa J202008
display id: BBa_J202008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PBAD Mutant#9
creator: Jiyeon Park
created: 2012-07-25T11:00:00Z
modified: 2015-08-31T04:08:37Z
discontinued: false
dominant: false
group u list: _910_
m user id: 0
owner id: 11164
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is PBAD (BBa_I13453) mutant. -60 relative to the araC transcriptional start site was changed from C to T.
mutable notes: In order to regulate transcription from PBAD, araC binds three different sites called araO2, araI1, and araI2. In the absence of L-arabinose, araC binds both araO2 and araI1 and bends DNA preventing transcription from PBAD. In the presence of L-arabinose, however, araC has a conformational change because of L-arabinose and binds araI1 and araI2, enabling transcription from PBAD. Like the wild-type (I13453), this part does not have araO2 site which is essential for araC repressor function. Therefore, araC acts as an activator in the presence of L-arabinose.
mutable provenance: This mutant was created by site-directed mutagenesis.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: araI1
sequence annotation: araI2
sequence annotation: Mutation
sequence annotation: Transcription Start
sequence: https://synbiohub.org/public/igem/BBa_J202008_sequence/1

araI1
type: SequenceAnnotation
persistent identity: annotation2178449
display id: annotation2178449
version: 1
top level: BBa_J202008
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J202008/annotation2178449/range2178449/1

https://synbiohub.org/public/igem/BBa_J202008/annotation2178449/range2178449/1
type: Range
persistent identity: range2178449
display id: range2178449
version: 1
direction: direction/forward
top level: BBa_J202008
start: 40
end: 56
orientation: inline

araI2
type: SequenceAnnotation
persistent identity: annotation2178450
display id: annotation2178450
version: 1
top level: BBa_J202008
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J202008/annotation2178450/range2178450/1

https://synbiohub.org/public/igem/BBa_J202008/annotation2178450/range2178450/1
type: Range
persistent identity: range2178450
display id: range2178450
version: 1
direction: direction/forward
top level: BBa_J202008
start: 61
end: 77
orientation: inline

Mutation
type: SequenceAnnotation
persistent identity: annotation2178448
display id: annotation2178448
version: 1
top level: BBa_J202008
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_J202008/annotation2178448/range2178448/1

https://synbiohub.org/public/igem/BBa_J202008/annotation2178448/range2178448/1
type: Range
persistent identity: range2178448
display id: range2178448
version: 1
direction: direction/forward
top level: BBa_J202008
start: 52
end: 52
orientation: inline

Transcription Start
type: SequenceAnnotation
persistent identity: annotation2178451
display id: annotation2178451
version: 1
top level: BBa_J202008
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_J202008/annotation2178451/range2178451/1

https://synbiohub.org/public/igem/BBa_J202008/annotation2178451/range2178451/1
type: Range
persistent identity: range2178451
display id: range2178451
version: 1
direction: direction/forward
top level: BBa_J202008
start: 112
end: 112
orientation: inline

BBa_K801010
type: ComponentDefinition
persistent identity: BBa K801010
display id: BBa_K801010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TEF2 constitutive yeast promoter
creator: Georg Sch??tzinger
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
discontinued: false
dominant: false
group u list: _1057_
m user id: 0
owner id: 13624
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Constitutive yeast promoter of medium strength compared to TEF1 promoter.
mutable notes: -
mutable provenance: Extracted from genomic DNA of ''S. cerevisiae'' using PCR.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: TEF1-Promoter
sequence: https://synbiohub.org/public/igem/BBa_K801010_sequence/1

TEF1-Promoter
type: SequenceAnnotation
persistent identity: annotation2202139
display id: annotation2202139
version: 1
top level: BBa_K801010
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K801010/annotation2202139/range2202139/1

https://synbiohub.org/public/igem/BBa_K801010/annotation2202139/range2202139/1
type: Range
persistent identity: range2202139
display id: range2202139
version: 1
direction: direction/forward
top level: BBa_K801010
start: 1
end: 647
orientation: inline

BBa_C0261
type: ComponentDefinition
persistent identity: BBa C0261
display id: BBa_C0261
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AHL-making Enzyme, luxI (+RBS)
creator: robertb
created: 2004-08-22T11:00:00Z
modified: 2015-08-31T04:07:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 159
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: B0034.C0061
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_C0061
component: BBa_B0034
sequence annotation: BBa_C0061
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_C0261_sequence/1

BBa_C0061
type: Component
persistent identity: component1060413
display id: component1060413
version: 1
top level: BBa_C0261
access: public
definition: luxI

BBa_B0034
type: Component
persistent identity: component1060403
display id: component1060403
version: 1
top level: BBa_C0261
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_C0061
type: SequenceAnnotation
persistent identity: annotation1060413
display id: annotation1060413
version: 1
top level: BBa_C0261
location: https://synbiohub.org/public/igem/BBa_C0261/annotation1060413/range1060413/1
component: BBa_C0061

https://synbiohub.org/public/igem/BBa_C0261/annotation1060413/range1060413/1
type: Range
persistent identity: range1060413
display id: range1060413
version: 1
direction: direction/forward
top level: BBa_C0261
start: 19
end: 636
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1060403
display id: annotation1060403
version: 1
top level: BBa_C0261
location: https://synbiohub.org/public/igem/BBa_C0261/annotation1060403/range1060403/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_C0261/annotation1060403/range1060403/1
type: Range
persistent identity: range1060403
display id: range1060403
version: 1
direction: direction/forward
top level: BBa_C0261
start: 1
end: 12
orientation: inline

BBa_J202006
type: ComponentDefinition
persistent identity: BBa J202006
display id: BBa_J202006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PBAD Mutant#7
creator: Jiyeon Park
created: 2012-07-25T11:00:00Z
modified: 2015-08-31T04:08:37Z
discontinued: false
dominant: false
group u list: _910_
m user id: 0
owner id: 11164
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is PBAD (BBa_I13453) mutant. -66 relative to the araC transcriptional start site was changed from T to G.
mutable notes: In order to regulate transcription from PBAD, araC binds three different sites called araO2, araI1, and araI2. In the absence of L-arabinose, araC binds both araO2 and araI1 and bends DNA preventing transcription from PBAD. In the presence of L-arabinose, however, araC has a conformational change because of L-arabinose and binds araI1 and araI2, enabling transcription from PBAD. Like the wild-type (I13453), this part does not have araO2 site which is essential for araC repressor function. Therefore, araC acts as an activator in the presence of L-arabinose.
mutable provenance: This mutant was created by site-directed mutagenesis.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Mutation
sequence annotation: araI2
sequence annotation: araI1
sequence annotation: Transcription Start
sequence: https://synbiohub.org/public/igem/BBa_J202006_sequence/1

Mutation
type: SequenceAnnotation
persistent identity: annotation2178443
display id: annotation2178443
version: 1
top level: BBa_J202006
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_J202006/annotation2178443/range2178443/1

https://synbiohub.org/public/igem/BBa_J202006/annotation2178443/range2178443/1
type: Range
persistent identity: range2178443
display id: range2178443
version: 1
direction: direction/forward
top level: BBa_J202006
start: 46
end: 46
orientation: inline

araI2
type: SequenceAnnotation
persistent identity: annotation2178442
display id: annotation2178442
version: 1
top level: BBa_J202006
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J202006/annotation2178442/range2178442/1

https://synbiohub.org/public/igem/BBa_J202006/annotation2178442/range2178442/1
type: Range
persistent identity: range2178442
display id: range2178442
version: 1
direction: direction/forward
top level: BBa_J202006
start: 61
end: 77
orientation: inline

araI1
type: SequenceAnnotation
persistent identity: annotation2178441
display id: annotation2178441
version: 1
top level: BBa_J202006
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J202006/annotation2178441/range2178441/1

https://synbiohub.org/public/igem/BBa_J202006/annotation2178441/range2178441/1
type: Range
persistent identity: range2178441
display id: range2178441
version: 1
direction: direction/forward
top level: BBa_J202006
start: 40
end: 56
orientation: inline

Transcription Start
type: SequenceAnnotation
persistent identity: annotation2178440
display id: annotation2178440
version: 1
top level: BBa_J202006
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_J202006/annotation2178440/range2178440/1

https://synbiohub.org/public/igem/BBa_J202006/annotation2178440/range2178440/1
type: Range
persistent identity: range2178440
display id: range2178440
version: 1
direction: direction/forward
top level: BBa_J202006
start: 112
end: 112
orientation: inline

BBa_K775010
type: ComponentDefinition
persistent identity: BBa K775010
display id: BBa_K775010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Isoamylacetate Chimeric GPCR with Fus1-EGFP reporter (2)
creator: Sietske Grijseels
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:16Z
discontinued: false
dominant: false
group u list: _1027_
m user id: 0
owner id: 12035
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K775004
component: BBa_K775001
sequence annotation: BBa_K775004
sequence annotation: BBa_K775001
sequence: https://synbiohub.org/public/igem/BBa_K775010_sequence/1

BBa_K775004
type: Component
persistent identity: component2195596
display id: component2195596
version: 1
top level: BBa_K775010
access: public
definition: https://synbiohub.org/public/igem/BBa_K775004/1

BBa_K775001
type: Component
persistent identity: component2195597
display id: component2195597
version: 1
top level: BBa_K775010
access: public
definition: BBa_K775001

BBa_K775004
type: SequenceAnnotation
persistent identity: annotation2195596
display id: annotation2195596
version: 1
top level: BBa_K775010
location: https://synbiohub.org/public/igem/BBa_K775010/annotation2195596/range2195596/1
component: BBa_K775004

https://synbiohub.org/public/igem/BBa_K775010/annotation2195596/range2195596/1
type: Range
persistent identity: range2195596
display id: range2195596
version: 1
direction: direction/reverse
top level: BBa_K775010
start: 1
end: 1166
orientation: inline

BBa_K775001
type: SequenceAnnotation
persistent identity: annotation2195597
display id: annotation2195597
version: 1
top level: BBa_K775010
location: https://synbiohub.org/public/igem/BBa_K775010/annotation2195597/range2195597/1
component: BBa_K775001

https://synbiohub.org/public/igem/BBa_K775010/annotation2195597/range2195597/1
type: Range
persistent identity: range2195597
display id: range2195597
version: 1
direction: direction/reverse
top level: BBa_K775010
start: 1175
end: 2597
orientation: inline

ecfp
type: ComponentDefinition
persistent identity: BBa I15016
display id: BBa_I15016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0032.ECFP
creator: Jeff Tabor
created: 2005-04-14T11:00:00Z
modified: 2015-08-31T04:07:38Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _5_
m user id: 0
owner id: 88
owning group id: 5
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part accepts POPS to produce ECFP under the medium RBS B0032
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_E0020
component: BBa_B0032
sequence annotation: BBa_E0020
sequence annotation: BBa_B0032
sequence: https://synbiohub.org/public/igem/BBa_I15016_sequence/1

BBa_E0020
type: Component
persistent identity: component1478408
display id: component1478408
version: 1
top level: ecfp
access: public
definition: ecfp

BBa_B0032
type: Component
persistent identity: component1478405
display id: component1478405
version: 1
top level: ecfp
access: public
definition: BBa_B0032

BBa_E0020
type: SequenceAnnotation
persistent identity: annotation1478408
display id: annotation1478408
version: 1
top level: ecfp
location: https://synbiohub.org/public/igem/BBa_I15016/annotation1478408/range1478408/1
component: BBa_E0020

https://synbiohub.org/public/igem/BBa_I15016/annotation1478408/range1478408/1
type: Range
persistent identity: range1478408
display id: range1478408
version: 1
direction: direction/forward
top level: ecfp
start: 20
end: 742
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1478405
display id: annotation1478405
version: 1
top level: ecfp
location: https://synbiohub.org/public/igem/BBa_I15016/annotation1478405/range1478405/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_I15016/annotation1478405/range1478405/1
type: Range
persistent identity: range1478405
display id: range1478405
version: 1
direction: direction/forward
top level: ecfp
start: 1
end: 13
orientation: inline

BBa_J202004
type: ComponentDefinition
persistent identity: BBa J202004
display id: BBa_J202004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PBAD Mutant#5
creator: Jiyeon Park
created: 2012-07-25T11:00:00Z
modified: 2015-08-31T04:08:37Z
discontinued: false
dominant: false
group u list: _910_
m user id: 0
owner id: 11164
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is PBAD (BBa_I13453) mutant. -71 relative to the araC transcriptional start site was changed from A to G.
mutable notes: In order to regulate transcription from PBAD, araC binds three different sites called araO2, araI1, and araI2. In the absence of L-arabinose, araC binds both araO2 and araI1 and bends DNA preventing transcription from PBAD. In the presence of L-arabinose, however, araC has a conformational change because of L-arabinose and binds araI1 and araI2, enabling transcription from PBAD. Like the wild-type (I13453), this part does not have araO2 site which is essential for araC repressor function. Therefore, araC acts as an activator in the presence of L-arabinose.
mutable provenance: This mutant was created by site-directed mutagenesis.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: araI2
sequence annotation: araI1
sequence annotation: Mutation
sequence annotation: Transcription Start
sequence: https://synbiohub.org/public/igem/BBa_J202004_sequence/1

araI2
type: SequenceAnnotation
persistent identity: annotation2178433
display id: annotation2178433
version: 1
top level: BBa_J202004
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J202004/annotation2178433/range2178433/1

https://synbiohub.org/public/igem/BBa_J202004/annotation2178433/range2178433/1
type: Range
persistent identity: range2178433
display id: range2178433
version: 1
direction: direction/forward
top level: BBa_J202004
start: 61
end: 77
orientation: inline

araI1
type: SequenceAnnotation
persistent identity: annotation2178432
display id: annotation2178432
version: 1
top level: BBa_J202004
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J202004/annotation2178432/range2178432/1

https://synbiohub.org/public/igem/BBa_J202004/annotation2178432/range2178432/1
type: Range
persistent identity: range2178432
display id: range2178432
version: 1
direction: direction/forward
top level: BBa_J202004
start: 40
end: 56
orientation: inline

Mutation
type: SequenceAnnotation
persistent identity: annotation2178435
display id: annotation2178435
version: 1
top level: BBa_J202004
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_J202004/annotation2178435/range2178435/1

https://synbiohub.org/public/igem/BBa_J202004/annotation2178435/range2178435/1
type: Range
persistent identity: range2178435
display id: range2178435
version: 1
direction: direction/forward
top level: BBa_J202004
start: 41
end: 41
orientation: inline

Transcription Start
type: SequenceAnnotation
persistent identity: annotation2178434
display id: annotation2178434
version: 1
top level: BBa_J202004
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_J202004/annotation2178434/range2178434/1

https://synbiohub.org/public/igem/BBa_J202004/annotation2178434/range2178434/1
type: Range
persistent identity: range2178434
display id: range2178434
version: 1
direction: direction/forward
top level: BBa_J202004
start: 112
end: 112
orientation: inline

BBa_K863010
type: ComponentDefinition
persistent identity: BBa K863010
display id: BBa_K863010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: tthl laccase from Thermus thermophilus with T7 promoter, RBS and His-tag
creator: Isabel Huber
created: 2012-09-17T11:00:00Z
modified: 2015-05-08T01:13:36Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1123_
m user id: 0
owner id: 12844
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: tthl (Laccase from Thermus thermophilus) with T7, RBS and HIS tag
mutable notes: test
mutable provenance: Thermus thermophilus HB27
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: TAA
sequence annotation: T7 promoter
sequence annotation: 6x HIS
sequence annotation: tthl
sequence annotation: ATG
sequence: https://synbiohub.org/public/igem/BBa_K863010_sequence/1

TAA
type: SequenceAnnotation
persistent identity: annotation2184783
display id: annotation2184783
version: 1
top level: BBa_K863010
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K863010/annotation2184783/range2184783/1

https://synbiohub.org/public/igem/BBa_K863010/annotation2184783/range2184783/1
type: Range
persistent identity: range2184783
display id: range2184783
version: 1
direction: direction/forward
top level: BBa_K863010
start: 1441
end: 1443
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2184708
display id: annotation2184708
version: 1
top level: BBa_K863010
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K863010/annotation2184708/range2184708/1

https://synbiohub.org/public/igem/BBa_K863010/annotation2184708/range2184708/1
type: Range
persistent identity: range2184708
display id: range2184708
version: 1
direction: direction/forward
top level: BBa_K863010
start: 1
end: 23
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2184782
display id: annotation2184782
version: 1
top level: BBa_K863010
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K863010/annotation2184782/range2184782/1

https://synbiohub.org/public/igem/BBa_K863010/annotation2184782/range2184782/1
type: Range
persistent identity: range2184782
display id: range2184782
version: 1
direction: direction/forward
top level: BBa_K863010
start: 1438
end: 1440
orientation: inline

6x HIS
type: SequenceAnnotation
persistent identity: annotation2184733
display id: annotation2184733
version: 1
top level: BBa_K863010
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K863010/annotation2184733/range2184733/1

https://synbiohub.org/public/igem/BBa_K863010/annotation2184733/range2184733/1
type: Range
persistent identity: range2184733
display id: range2184733
version: 1
direction: direction/forward
top level: BBa_K863010
start: 1420
end: 1437
orientation: inline

tthl
type: SequenceAnnotation
persistent identity: annotation2184732
display id: annotation2184732
version: 1
top level: BBa_K863010
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K863010/annotation2184732/range2184732/1

https://synbiohub.org/public/igem/BBa_K863010/annotation2184732/range2184732/1
type: Range
persistent identity: range2184732
display id: range2184732
version: 1
direction: direction/forward
top level: BBa_K863010
start: 34
end: 1419
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2184671
display id: annotation2184671
version: 1
top level: BBa_K863010
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K863010/annotation2184671/range2184671/1

https://synbiohub.org/public/igem/BBa_K863010/annotation2184671/range2184671/1
type: Range
persistent identity: range2184671
display id: range2184671
version: 1
direction: direction/forward
top level: BBa_K863010
start: 34
end: 36
orientation: inline

BBa_I6066
type: ComponentDefinition
persistent identity: BBa I6066
display id: BBa_I6066
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter O_H Test R0051.E0432
creator: Caitlin Conboy and Jennifer Braff
created: 2004-03-17T12:00:00Z
modified: 2015-08-31T04:07:43Z
discontinued: false
dominant: true
experience: experience/Issues
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_B0010
component: BBa_B0034
component: BBa_E0032
component: BBa_B0012
component: BBa_R0051
sequence annotation: BBa_R0051
sequence annotation: BBa_B0034
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_E0032
sequence: https://synbiohub.org/public/igem/BBa_I6066_sequence/1

BBa_B0010
type: Component
persistent identity: component949110
display id: component949110
version: 1
top level: BBa_I6066
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component949090
display id: component949090
version: 1
top level: BBa_I6066
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_E0032
type: Component
persistent identity: component949102
display id: component949102
version: 1
top level: BBa_I6066
access: public
definition: YFP

BBa_B0012
type: Component
persistent identity: component949120
display id: component949120
version: 1
top level: BBa_I6066
access: public
definition: BBa_B0012

BBa_R0051
type: Component
persistent identity: component949082
display id: component949082
version: 1
top level: BBa_I6066
access: public
definition: https://synbiohub.org/public/igem/BBa_R0051/1

BBa_R0051
type: SequenceAnnotation
persistent identity: annotation949082
display id: annotation949082
version: 1
top level: BBa_I6066
location: https://synbiohub.org/public/igem/BBa_I6066/annotation949082/range949082/1
component: BBa_R0051

https://synbiohub.org/public/igem/BBa_I6066/annotation949082/range949082/1
type: Range
persistent identity: range949082
display id: range949082
version: 1
direction: direction/forward
top level: BBa_I6066
start: 1
end: 49
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation949090
display id: annotation949090
version: 1
top level: BBa_I6066
location: https://synbiohub.org/public/igem/BBa_I6066/annotation949090/range949090/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I6066/annotation949090/range949090/1
type: Range
persistent identity: range949090
display id: range949090
version: 1
direction: direction/forward
top level: BBa_I6066
start: 58
end: 69
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation949120
display id: annotation949120
version: 1
top level: BBa_I6066
location: https://synbiohub.org/public/igem/BBa_I6066/annotation949120/range949120/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I6066/annotation949120/range949120/1
type: Range
persistent identity: range949120
display id: range949120
version: 1
direction: direction/forward
top level: BBa_I6066
start: 934
end: 974
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation949110
display id: annotation949110
version: 1
top level: BBa_I6066
location: https://synbiohub.org/public/igem/BBa_I6066/annotation949110/range949110/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I6066/annotation949110/range949110/1
type: Range
persistent identity: range949110
display id: range949110
version: 1
direction: direction/forward
top level: BBa_I6066
start: 846
end: 925
orientation: inline

BBa_E0032
type: SequenceAnnotation
persistent identity: annotation949102
display id: annotation949102
version: 1
top level: BBa_I6066
location: https://synbiohub.org/public/igem/BBa_I6066/annotation949102/range949102/1
component: BBa_E0032

https://synbiohub.org/public/igem/BBa_I6066/annotation949102/range949102/1
type: Range
persistent identity: range949102
display id: range949102
version: 1
direction: direction/forward
top level: BBa_I6066
start: 76
end: 837
orientation: inline

BBa_I13523
type: ComponentDefinition
persistent identity: BBa I13523
display id: BBa_I13523
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Screening plasmid v2
creator: jkm
created: 2005-07-18T11:00:00Z
modified: 2015-08-31T04:07:35Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Intermediate in screening plasmid v2 construction.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_B0010
component: BBa_B0034
component: BBa_E0040
component: BBa_B0012
component: BBa_I13455
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_I13455
sequence annotation: BBa_B0034
sequence annotation: BBa_E0040
sequence: https://synbiohub.org/public/igem/BBa_I13523_sequence/1

BBa_B0010
type: Component
persistent identity: component1597485
display id: component1597485
version: 1
top level: BBa_I13523
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component1597478
display id: component1597478
version: 1
top level: BBa_I13523
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_E0040
type: Component
persistent identity: component1597480
display id: component1597480
version: 1
top level: BBa_I13523
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_B0012
type: Component
persistent identity: component1597495
display id: component1597495
version: 1
top level: BBa_I13523
access: public
definition: BBa_B0012

BBa_I13455
type: Component
persistent identity: component1597473
display id: component1597473
version: 1
top level: BBa_I13523
access: public
definition: https://synbiohub.org/public/igem/BBa_I13455/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1597495
display id: annotation1597495
version: 1
top level: BBa_I13523
location: https://synbiohub.org/public/igem/BBa_I13523/annotation1597495/range1597495/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13523/annotation1597495/range1597495/1
type: Range
persistent identity: range1597495
display id: range1597495
version: 1
direction: direction/forward
top level: BBa_I13523
start: 921
end: 961
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1597485
display id: annotation1597485
version: 1
top level: BBa_I13523
location: https://synbiohub.org/public/igem/BBa_I13523/annotation1597485/range1597485/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13523/annotation1597485/range1597485/1
type: Range
persistent identity: range1597485
display id: range1597485
version: 1
direction: direction/forward
top level: BBa_I13523
start: 833
end: 912
orientation: inline

BBa_I13455
type: SequenceAnnotation
persistent identity: annotation1597473
display id: annotation1597473
version: 1
top level: BBa_I13523
location: https://synbiohub.org/public/igem/BBa_I13523/annotation1597473/range1597473/1
component: BBa_I13455

https://synbiohub.org/public/igem/BBa_I13523/annotation1597473/range1597473/1
type: Range
persistent identity: range1597473
display id: range1597473
version: 1
direction: direction/forward
top level: BBa_I13523
start: 1
end: 78
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1597478
display id: annotation1597478
version: 1
top level: BBa_I13523
location: https://synbiohub.org/public/igem/BBa_I13523/annotation1597478/range1597478/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13523/annotation1597478/range1597478/1
type: Range
persistent identity: range1597478
display id: range1597478
version: 1
direction: direction/forward
top level: BBa_I13523
start: 87
end: 98
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1597480
display id: annotation1597480
version: 1
top level: BBa_I13523
location: https://synbiohub.org/public/igem/BBa_I13523/annotation1597480/range1597480/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_I13523/annotation1597480/range1597480/1
type: Range
persistent identity: range1597480
display id: range1597480
version: 1
direction: direction/forward
top level: BBa_I13523
start: 105
end: 824
orientation: inline

BBa_K801071
type: ComponentDefinition
persistent identity: BBa K801071
display id: BBa_K801071
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 7-methylxanthine N-methyltransferase CaMXMT1-strep
creator: Roman Prechtl
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1057_
m user id: 0
owner id: 11966
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: 7-methylxanthine N-methyltransferase CaMXMT1 from ''Coffea arabica'' followed by a strep-tag for purification and/or detection via western blot.
mutable notes: -
mutable provenance: Synthesized by IDT, Yeast codon optimized
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Strep Tag
sequence annotation: CaMXMT1
sequence annotation: Start codon
sequence annotation: Consensus sequence
sequence annotation: Stop codon
sequence: https://synbiohub.org/public/igem/BBa_K801071_sequence/1

Strep Tag
type: SequenceAnnotation
persistent identity: annotation2198148
display id: annotation2198148
version: 1
top level: BBa_K801071
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K801071/annotation2198148/range2198148/1

https://synbiohub.org/public/igem/BBa_K801071/annotation2198148/range2198148/1
type: Range
persistent identity: range2198148
display id: range2198148
version: 1
direction: direction/forward
top level: BBa_K801071
start: 1141
end: 1170
orientation: inline

CaMXMT1
type: SequenceAnnotation
persistent identity: annotation2197709
display id: annotation2197709
version: 1
top level: BBa_K801071
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K801071/annotation2197709/range2197709/1

https://synbiohub.org/public/igem/BBa_K801071/annotation2197709/range2197709/1
type: Range
persistent identity: range2197709
display id: range2197709
version: 1
direction: direction/forward
top level: BBa_K801071
start: 7
end: 1140
orientation: inline

Start codon
type: SequenceAnnotation
persistent identity: annotation2198586
display id: annotation2198586
version: 1
top level: BBa_K801071
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K801071/annotation2198586/range2198586/1

https://synbiohub.org/public/igem/BBa_K801071/annotation2198586/range2198586/1
type: Range
persistent identity: range2198586
display id: range2198586
version: 1
direction: direction/forward
top level: BBa_K801071
start: 7
end: 9
orientation: inline

Consensus sequence
type: SequenceAnnotation
persistent identity: annotation2197876
display id: annotation2197876
version: 1
top level: BBa_K801071
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801071/annotation2197876/range2197876/1

https://synbiohub.org/public/igem/BBa_K801071/annotation2197876/range2197876/1
type: Range
persistent identity: range2197876
display id: range2197876
version: 1
direction: direction/forward
top level: BBa_K801071
start: 1
end: 6
orientation: inline

Stop codon
type: SequenceAnnotation
persistent identity: annotation2198587
display id: annotation2198587
version: 1
top level: BBa_K801071
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K801071/annotation2198587/range2198587/1

https://synbiohub.org/public/igem/BBa_K801071/annotation2198587/range2198587/1
type: Range
persistent identity: range2198587
display id: range2198587
version: 1
direction: direction/forward
top level: BBa_K801071
start: 1171
end: 1176
orientation: inline

BBa_K561000
type: ComponentDefinition
persistent identity: BBa K561000
display id: BBa_K561000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pvgb+YFP+tetR
creator: HUA Yijie, PI Ruoxi, XIA Yu
created: 2011-09-28T11:00:00Z
modified: 2015-05-08T01:12:41Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _729_
m user id: 0
owner id: 8637
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: The expression of YFP and tetR is under the control of microaerobic promoter vgb. The expression of tetR will repress Ptet.
mutable notes: Because vgb promoter induces high expression under microaerobic conditions and induces lower expression under anaerobic conditions, we used medium RBS to resist it from inducing expression under anaerobic conditions.
mutable provenance: vgb promoter comes from Vitreoscilla sp.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K561001
component: BBa_P0440
component: BBa_I15017
sequence annotation: BBa_I15017
sequence annotation: BBa_K561001
sequence annotation: BBa_P0440
sequence: https://synbiohub.org/public/igem/BBa_K561000_sequence/1

BBa_K561001
type: Component
persistent identity: component2146962
display id: component2146962
version: 1
top level: BBa_K561000
access: public
definition: https://synbiohub.org/public/igem/BBa_K561001/1

BBa_P0440
type: Component
persistent identity: component2146980
display id: component2146980
version: 1
top level: BBa_K561000
access: public
definition: BBa_P0440

BBa_I15017
type: Component
persistent identity: component2146967
display id: component2146967
version: 1
top level: BBa_K561000
access: public
definition: ecfp

BBa_I15017
type: SequenceAnnotation
persistent identity: annotation2146967
display id: annotation2146967
version: 1
top level: BBa_K561000
location: https://synbiohub.org/public/igem/BBa_K561000/annotation2146967/range2146967/1
component: BBa_I15017

https://synbiohub.org/public/igem/BBa_K561000/annotation2146967/range2146967/1
type: Range
persistent identity: range2146967
display id: range2146967
version: 1
direction: direction/reverse
top level: BBa_K561000
start: 144
end: 885
orientation: inline

BBa_K561001
type: SequenceAnnotation
persistent identity: annotation2146962
display id: annotation2146962
version: 1
top level: BBa_K561000
location: https://synbiohub.org/public/igem/BBa_K561000/annotation2146962/range2146962/1
component: BBa_K561001

https://synbiohub.org/public/igem/BBa_K561000/annotation2146962/range2146962/1
type: Range
persistent identity: range2146962
display id: range2146962
version: 1
direction: direction/reverse
top level: BBa_K561000
start: 1
end: 135
orientation: inline

BBa_P0440
type: SequenceAnnotation
persistent identity: annotation2146980
display id: annotation2146980
version: 1
top level: BBa_K561000
location: https://synbiohub.org/public/igem/BBa_K561000/annotation2146980/range2146980/1
component: BBa_P0440

https://synbiohub.org/public/igem/BBa_K561000/annotation2146980/range2146980/1
type: Range
persistent identity: range2146980
display id: range2146980
version: 1
direction: direction/reverse
top level: BBa_K561000
start: 894
end: 1733
orientation: inline

BBa_K574006
type: ComponentDefinition
persistent identity: BBa K574006
display id: BBa_K574006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 3OC12HSL regulated by pTet and pBad
creator: Lei Wei
created: 2011-09-28T11:00:00Z
modified: 2015-05-08T01:12:44Z
discontinued: false
dominant: false
experience: experience/None
group u list: _745_
m user id: 0
owner id: 8529
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Part 2/3 of a Oscillator.
mutable notes: Empty.
mutable provenance: Existing Parts.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K574004
component: BBa_K574005
sequence annotation: BBa_K574005
sequence annotation: BBa_K574004
sequence: https://synbiohub.org/public/igem/BBa_K574006_sequence/1

BBa_K574004
type: Component
persistent identity: component2295487
display id: component2295487
version: 1
top level: BBa_K574006
access: public
definition: https://synbiohub.org/public/igem/BBa_K574004/1

BBa_K574005
type: Component
persistent identity: component2295507
display id: component2295507
version: 1
top level: BBa_K574006
access: public
definition: BBa_K574005

BBa_K574005
type: SequenceAnnotation
persistent identity: annotation2295507
display id: annotation2295507
version: 1
top level: BBa_K574006
location: https://synbiohub.org/public/igem/BBa_K574006/annotation2295507/range2295507/1
component: BBa_K574005

https://synbiohub.org/public/igem/BBa_K574006/annotation2295507/range2295507/1
type: Range
persistent identity: range2295507
display id: range2295507
version: 1
direction: direction/reverse
top level: BBa_K574006
start: 806
end: 2517
orientation: inline

BBa_K574004
type: SequenceAnnotation
persistent identity: annotation2295487
display id: annotation2295487
version: 1
top level: BBa_K574006
location: https://synbiohub.org/public/igem/BBa_K574006/annotation2295487/range2295487/1
component: BBa_K574004

https://synbiohub.org/public/igem/BBa_K574006/annotation2295487/range2295487/1
type: Range
persistent identity: range2295487
display id: range2295487
version: 1
direction: direction/reverse
top level: BBa_K574006
start: 1
end: 797
orientation: inline

BBa_J202003
type: ComponentDefinition
persistent identity: BBa J202003
display id: BBa_J202003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PBAD Mutant#4
creator: Jiyeon Park
created: 2012-07-25T11:00:00Z
modified: 2015-08-31T04:08:37Z
discontinued: false
dominant: false
group u list: _910_
m user id: 0
owner id: 11164
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is PBAD (BBa_I13453) mutant. -63(T to C), -42 (C to A), and +9 (T to A) relative to the araC transcriptional start site were changed.
mutable notes: In order to regulate transcription from PBAD, araC binds three different sites called araO2, araI1, and araI2. In the absence of L-arabinose, araC binds both araO2 and araI1 and bends DNA preventing transcription from PBAD. In the presence of L-arabinose, however, araC has a conformational change because of L-arabinose and binds araI1 and araI2, enabling transcription from PBAD. Like the wild-type (I13453), this part does not have araO2 site which is essential for araC repressor function. Therefore, araC acts as an activator in the presence of L-arabinose.
mutable provenance: This mutant was created by random mutagenesis.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: araI2
sequence annotation: Mutation 3
sequence annotation: Mutation 2
sequence annotation: Transcription Start
sequence annotation: araI1
sequence annotation: Mutation 1
sequence: https://synbiohub.org/public/igem/BBa_J202003_sequence/1

araI2
type: SequenceAnnotation
persistent identity: annotation2178428
display id: annotation2178428
version: 1
top level: BBa_J202003
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J202003/annotation2178428/range2178428/1

https://synbiohub.org/public/igem/BBa_J202003/annotation2178428/range2178428/1
type: Range
persistent identity: range2178428
display id: range2178428
version: 1
direction: direction/forward
top level: BBa_J202003
start: 61
end: 77
orientation: inline

Mutation 3
type: SequenceAnnotation
persistent identity: annotation2178431
display id: annotation2178431
version: 1
top level: BBa_J202003
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_J202003/annotation2178431/range2178431/1

https://synbiohub.org/public/igem/BBa_J202003/annotation2178431/range2178431/1
type: Range
persistent identity: range2178431
display id: range2178431
version: 1
direction: direction/forward
top level: BBa_J202003
start: 120
end: 120
orientation: inline

Mutation 2
type: SequenceAnnotation
persistent identity: annotation2178430
display id: annotation2178430
version: 1
top level: BBa_J202003
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_J202003/annotation2178430/range2178430/1

https://synbiohub.org/public/igem/BBa_J202003/annotation2178430/range2178430/1
type: Range
persistent identity: range2178430
display id: range2178430
version: 1
direction: direction/forward
top level: BBa_J202003
start: 70
end: 70
orientation: inline

Transcription Start
type: SequenceAnnotation
persistent identity: annotation2178426
display id: annotation2178426
version: 1
top level: BBa_J202003
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_J202003/annotation2178426/range2178426/1

https://synbiohub.org/public/igem/BBa_J202003/annotation2178426/range2178426/1
type: Range
persistent identity: range2178426
display id: range2178426
version: 1
direction: direction/forward
top level: BBa_J202003
start: 112
end: 112
orientation: inline

araI1
type: SequenceAnnotation
persistent identity: annotation2178427
display id: annotation2178427
version: 1
top level: BBa_J202003
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J202003/annotation2178427/range2178427/1

https://synbiohub.org/public/igem/BBa_J202003/annotation2178427/range2178427/1
type: Range
persistent identity: range2178427
display id: range2178427
version: 1
direction: direction/forward
top level: BBa_J202003
start: 40
end: 56
orientation: inline

Mutation 1
type: SequenceAnnotation
persistent identity: annotation2178429
display id: annotation2178429
version: 1
top level: BBa_J202003
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_J202003/annotation2178429/range2178429/1

https://synbiohub.org/public/igem/BBa_J202003/annotation2178429/range2178429/1
type: Range
persistent identity: range2178429
display id: range2178429
version: 1
direction: direction/forward
top level: BBa_J202003
start: 49
end: 49
orientation: inline

BBa_K863020
type: ComponentDefinition
persistent identity: BBa K863020
display id: BBa_K863020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: bhal laccase from Bacillus halodurans with T7 promoter, RBS and His-tag
creator: Isabel Huber
created: 2012-09-18T11:00:00Z
modified: 2015-05-08T01:13:36Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1123_
m user id: 0
owner id: 12844
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: bhal laccase from Bacillus halodurans with T7 and HIS
mutable notes: test
mutable provenance: Bacillus halodurans C-125
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: TAA
sequence annotation: bhal
sequence annotation: 6 x HIS
sequence annotation: T7
sequence annotation: RBS
sequence annotation: ATG
sequence: https://synbiohub.org/public/igem/BBa_K863020_sequence/1

TAA
type: SequenceAnnotation
persistent identity: annotation2202256
display id: annotation2202256
version: 1
top level: BBa_K863020
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K863020/annotation2202256/range2202256/1

https://synbiohub.org/public/igem/BBa_K863020/annotation2202256/range2202256/1
type: Range
persistent identity: range2202256
display id: range2202256
version: 1
direction: direction/forward
top level: BBa_K863020
start: 1552
end: 1554
orientation: inline

bhal
type: SequenceAnnotation
persistent identity: annotation2202254
display id: annotation2202254
version: 1
top level: BBa_K863020
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K863020/annotation2202254/range2202254/1

https://synbiohub.org/public/igem/BBa_K863020/annotation2202254/range2202254/1
type: Range
persistent identity: range2202254
display id: range2202254
version: 1
direction: direction/forward
top level: BBa_K863020
start: 34
end: 1533
orientation: inline

6 x HIS
type: SequenceAnnotation
persistent identity: annotation2202255
display id: annotation2202255
version: 1
top level: BBa_K863020
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K863020/annotation2202255/range2202255/1

https://synbiohub.org/public/igem/BBa_K863020/annotation2202255/range2202255/1
type: Range
persistent identity: range2202255
display id: range2202255
version: 1
direction: direction/forward
top level: BBa_K863020
start: 1534
end: 1551
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2202257
display id: annotation2202257
version: 1
top level: BBa_K863020
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K863020/annotation2202257/range2202257/1

https://synbiohub.org/public/igem/BBa_K863020/annotation2202257/range2202257/1
type: Range
persistent identity: range2202257
display id: range2202257
version: 1
direction: direction/forward
top level: BBa_K863020
start: 1555
end: 1557
orientation: inline

T7
type: SequenceAnnotation
persistent identity: annotation2202251
display id: annotation2202251
version: 1
top level: BBa_K863020
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K863020/annotation2202251/range2202251/1

https://synbiohub.org/public/igem/BBa_K863020/annotation2202251/range2202251/1
type: Range
persistent identity: range2202251
display id: range2202251
version: 1
direction: direction/forward
top level: BBa_K863020
start: 1
end: 23
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2202252
display id: annotation2202252
version: 1
top level: BBa_K863020
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K863020/annotation2202252/range2202252/1

https://synbiohub.org/public/igem/BBa_K863020/annotation2202252/range2202252/1
type: Range
persistent identity: range2202252
display id: range2202252
version: 1
direction: direction/forward
top level: BBa_K863020
start: 25
end: 30
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2202253
display id: annotation2202253
version: 1
top level: BBa_K863020
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K863020/annotation2202253/range2202253/1

https://synbiohub.org/public/igem/BBa_K863020/annotation2202253/range2202253/1
type: Range
persistent identity: range2202253
display id: range2202253
version: 1
direction: direction/forward
top level: BBa_K863020
start: 34
end: 36
orientation: inline

BBa_K863204
type: ComponentDefinition
persistent identity: BBa K863204
display id: BBa_K863204
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: shuttle vector pECPP11JS for recombination of genes of interest in yeast
creator: Julia Schirmacher
created: 2012-09-22T11:00:00Z
modified: 2015-05-08T01:13:36Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1123_
m user id: 0
owner id: 13076
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: long description is coming soon
mutable notes: the design considerations are coming soon
mutable provenance: This part is made of the BioBricks XYZ by gibson assembly.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: 5' AOX
sequence annotation: Kosak sequence
sequence annotation: MFalpha
sequence annotation: 3' AOX
sequence annotation: AOX1 terminator
sequence annotation: his4
sequence: https://synbiohub.org/public/igem/BBa_K863204_sequence/1

5' AOX
type: SequenceAnnotation
persistent identity: annotation2200002
display id: annotation2200002
version: 1
top level: BBa_K863204
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K863204/annotation2200002/range2200002/1

https://synbiohub.org/public/igem/BBa_K863204/annotation2200002/range2200002/1
type: Range
persistent identity: range2200002
display id: range2200002
version: 1
direction: direction/forward
top level: BBa_K863204
start: 1
end: 940
orientation: inline

Kosak sequence
type: SequenceAnnotation
persistent identity: annotation2200003
display id: annotation2200003
version: 1
top level: BBa_K863204
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K863204/annotation2200003/range2200003/1

https://synbiohub.org/public/igem/BBa_K863204/annotation2200003/range2200003/1
type: Range
persistent identity: range2200003
display id: range2200003
version: 1
direction: direction/forward
top level: BBa_K863204
start: 941
end: 952
orientation: inline

MFalpha
type: SequenceAnnotation
persistent identity: annotation2200004
display id: annotation2200004
version: 1
top level: BBa_K863204
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K863204/annotation2200004/range2200004/1

https://synbiohub.org/public/igem/BBa_K863204/annotation2200004/range2200004/1
type: Range
persistent identity: range2200004
display id: range2200004
version: 1
direction: direction/forward
top level: BBa_K863204
start: 953
end: 1215
orientation: inline

3' AOX
type: SequenceAnnotation
persistent identity: annotation2200007
display id: annotation2200007
version: 1
top level: BBa_K863204
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K863204/annotation2200007/range2200007/1

https://synbiohub.org/public/igem/BBa_K863204/annotation2200007/range2200007/1
type: Range
persistent identity: range2200007
display id: range2200007
version: 1
direction: direction/forward
top level: BBa_K863204
start: 4184
end: 4867
orientation: inline

AOX1 terminator
type: SequenceAnnotation
persistent identity: annotation2200005
display id: annotation2200005
version: 1
top level: BBa_K863204
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K863204/annotation2200005/range2200005/1

https://synbiohub.org/public/igem/BBa_K863204/annotation2200005/range2200005/1
type: Range
persistent identity: range2200005
display id: range2200005
version: 1
direction: direction/forward
top level: BBa_K863204
start: 1250
end: 1531
orientation: inline

his4
type: SequenceAnnotation
persistent identity: annotation2200006
display id: annotation2200006
version: 1
top level: BBa_K863204
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K863204/annotation2200006/range2200006/1

https://synbiohub.org/public/igem/BBa_K863204/annotation2200006/range2200006/1
type: Range
persistent identity: range2200006
display id: range2200006
version: 1
direction: direction/forward
top level: BBa_K863204
start: 1615
end: 4143
orientation: inline

SV40 NLS
type: ComponentDefinition
persistent identity: BBa J63008
display id: BBa_J63008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SV40 nuclear localization sequence from SV40; yeast codon optimized
creator: Caroline Ajo-Franklin
created: 2006-10-10T11:00:00Z
modified: 2015-08-31T01:56:26Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _97_
m user id: 0
owner id: 545
owning group id: 97
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: yeast codon-optimized nuclear localization sequence from simian virus 40
mutable notes: as described in literature
mutable provenance: direct synthesis from published sequence
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence: https://synbiohub.org/public/igem/BBa_J63008_sequence/1

BBa_K863005
type: ComponentDefinition
persistent identity: BBa K863005
display id: BBa_K863005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ecol laccase from E. coli with T7 promoter, RBS and His-tag
creator: Isabel Huber
created: 2012-09-17T11:00:00Z
modified: 2015-05-08T01:13:36Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1123_
m user id: 0
owner id: 12844
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: E.coli laccase ORF with T7, RBS and HIS tag
mutable notes: TEST
mutable provenance: E. coli BL21 (DE3)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: TAA
sequence annotation: ATG
sequence annotation: ecol
sequence annotation: RBS
sequence annotation: 6x HIS
sequence annotation: T7 promoter
sequence: https://synbiohub.org/public/igem/BBa_K863005_sequence/1

TAA
type: SequenceAnnotation
persistent identity: annotation2184564
display id: annotation2184564
version: 1
top level: BBa_K863005
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K863005/annotation2184564/range2184564/1

https://synbiohub.org/public/igem/BBa_K863005/annotation2184564/range2184564/1
type: Range
persistent identity: range2184564
display id: range2184564
version: 1
direction: direction/forward
top level: BBa_K863005
start: 1600
end: 1602
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2184563
display id: annotation2184563
version: 1
top level: BBa_K863005
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K863005/annotation2184563/range2184563/1

https://synbiohub.org/public/igem/BBa_K863005/annotation2184563/range2184563/1
type: Range
persistent identity: range2184563
display id: range2184563
version: 1
direction: direction/forward
top level: BBa_K863005
start: 1603
end: 1605
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2184562
display id: annotation2184562
version: 1
top level: BBa_K863005
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K863005/annotation2184562/range2184562/1

https://synbiohub.org/public/igem/BBa_K863005/annotation2184562/range2184562/1
type: Range
persistent identity: range2184562
display id: range2184562
version: 1
direction: direction/forward
top level: BBa_K863005
start: 34
end: 36
orientation: inline

ecol
type: SequenceAnnotation
persistent identity: annotation2184566
display id: annotation2184566
version: 1
top level: BBa_K863005
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K863005/annotation2184566/range2184566/1

https://synbiohub.org/public/igem/BBa_K863005/annotation2184566/range2184566/1
type: Range
persistent identity: range2184566
display id: range2184566
version: 1
direction: direction/forward
top level: BBa_K863005
start: 34
end: 1581
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2187301
display id: annotation2187301
version: 1
top level: BBa_K863005
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K863005/annotation2187301/range2187301/1

https://synbiohub.org/public/igem/BBa_K863005/annotation2187301/range2187301/1
type: Range
persistent identity: range2187301
display id: range2187301
version: 1
direction: direction/forward
top level: BBa_K863005
start: 25
end: 30
orientation: inline

6x HIS
type: SequenceAnnotation
persistent identity: annotation2184565
display id: annotation2184565
version: 1
top level: BBa_K863005
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K863005/annotation2184565/range2184565/1

https://synbiohub.org/public/igem/BBa_K863005/annotation2184565/range2184565/1
type: Range
persistent identity: range2184565
display id: range2184565
version: 1
direction: direction/forward
top level: BBa_K863005
start: 1582
end: 1599
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2184561
display id: annotation2184561
version: 1
top level: BBa_K863005
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K863005/annotation2184561/range2184561/1

https://synbiohub.org/public/igem/BBa_K863005/annotation2184561/range2184561/1
type: Range
persistent identity: range2184561
display id: range2184561
version: 1
direction: direction/forward
top level: BBa_K863005
start: 1
end: 23
orientation: inline

HMG-CoA-R
type: ComponentDefinition
persistent identity: BBa K849000
display id: BBa_K849000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 3-Hydroxy-3-Methyl-Glutaryl-CoA-Reductase from Yeast
creator: Leonard Sebastian Fresenborg
created: 2012-09-19T11:00:00Z
modified: 2015-05-08T01:13:34Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1109_
m user id: 0
owner id: 12733
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This is a truncated version of the 3-Hydroxy-3-Methyl-Glutaryl-Coenzyme-A-Reductase (HMG-CoA-R) from ''Saccharomyces cerevisiae''. It catalyses the reaction of 3-Hydroxy-3-Methyl-Glutaryle-Coenzyme-A to Mevalonate which occurs in the Mevalonate Pathway to Isopentylpyrophosphate which is the Precurser of all Isoprenoides. The enzyme is naturally a membrane bound enzyme. The membrane anchor is situated N-terminal. This region is also essential for the degradational control of the enzyme. By deleting the N-terminal part to Aminoacid (inclusive) the enzyme is expected to be water soluble and deregulated.
mutable notes: The truncation was done according to the following publication: Rico, Pardo, Orejas: 'Enhanced Production of a Plant Monoterpene by Overexpression of the 3-Hydroxy-3-Methylglutaryl Conenzyme A Reductase Catalytic Domain in Saccharomyces cerevisiae'; Applied and Enviromental Microbiology; Vol 76 p. 6449-6454; 2010
mutable provenance: The original source of this part is the genome of Saccharomyces cerevisiae. We obtained the sequence by de novo synthesis. For that we used data from the Saccharomyes Genome Database. We inserted some calm mutations to avoid conflicts with the Cloning Standard 10.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: cds
sequence: https://synbiohub.org/public/igem/BBa_K849000_sequence/1

cds
type: SequenceAnnotation
persistent identity: annotation2202427
display id: annotation2202427
version: 1
top level: HMG-CoA-R
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K849000/annotation2202427/range2202427/1

https://synbiohub.org/public/igem/BBa_K849000/annotation2202427/range2202427/1
type: Range
persistent identity: range2202427
display id: range2202427
version: 1
direction: direction/forward
top level: HMG-CoA-R
start: 1
end: 1584
orientation: inline

BBa_I13500
type: ComponentDefinition
persistent identity: BBa I13500
display id: BBa_I13500
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Screening plasmid intermediate
creator: jkm
created: 2005-05-30T11:00:00Z
modified: 2015-08-31T04:07:34Z
discontinued: false
dominant: true
experience: experience/None
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Built by Josh as an intermediate in screening plasmid construction
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_E0040
component: BBa_B0034
sequence annotation: BBa_E0040
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_I13500_sequence/1

BBa_E0040
type: Component
persistent identity: component1505002
display id: component1505002
version: 1
top level: BBa_I13500
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_B0034
type: Component
persistent identity: component1505000
display id: component1505000
version: 1
top level: BBa_I13500
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1505002
display id: annotation1505002
version: 1
top level: BBa_I13500
location: https://synbiohub.org/public/igem/BBa_I13500/annotation1505002/range1505002/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_I13500/annotation1505002/range1505002/1
type: Range
persistent identity: range1505002
display id: range1505002
version: 1
direction: direction/forward
top level: BBa_I13500
start: 19
end: 738
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1505000
display id: annotation1505000
version: 1
top level: BBa_I13500
location: https://synbiohub.org/public/igem/BBa_I13500/annotation1505000/range1505000/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13500/annotation1505000/range1505000/1
type: Range
persistent identity: range1505000
display id: range1505000
version: 1
direction: direction/forward
top level: BBa_I13500
start: 1
end: 12
orientation: inline

BBa_J61016
type: ComponentDefinition
persistent identity: BBa J61016
display id: BBa_J61016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Biobrick converter for BamHI-part-EcoRI
creator: John Anderson
created: 2006-08-10T11:00:00Z
modified: 2015-08-31T02:02:59Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Inserting a BamHI/EcoRI fragment into the BglII/MfeI sites of J61015 destroys the restriction sites and introduces standard Biobrick flanking sequence. In the process, the OnRFP cassette is destroyed allowing Red to White screening of colonies.
mutable notes: N/A
mutable provenance: PCR ca1007F/R on pSB1A2-J01064 (972 bp, EcoRI/SpeI) PCR ca1008F/ca1009R on pSB1A2-J01064 (972 bp, EcoRI/SpeI) PCR ca1007F/ca1009R on pSB1A2-J01064 (972 bp, EcoRI/SpeI) Sub into pSB1A2 (EcoRI/SpeI) Products are: Bca1007 NotI/BamHI transfer Bca1008 BamHI/EcoRI transfer Bca1009 NotI/EcoRI transfer ------------------------------------------------ ca1007F Forward EcoRI for 5' PspOMI NotI converter ctctggaattcgcggccgcttctagagggccctccctatcagtgatagagattg ca1007R Reverse SpeI for 3' BglII BamHI converter ccgctactagtagatcttataaacgcagaaaggcccac ca1008F Forward EcoRI for 5' BglII BamHI converter ctctggaattcgcggccgcttctagaagatcttccctatcagtgatagagattg ca1009R Reverse SpeI for 3' MfeI EcoRI converter ccgctactagtcaattgtataaacgcagaaaggcccac
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_J61016_sequence/1

BBa_K598020
type: ComponentDefinition
persistent identity: BBa K598020
display id: BBa_K598020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0015+pBAD+vioAB+B0015+pBAD+TPP Up-regulated Hammerhead Ribozyme 1.20+vioD+B0015+pBAD+vioE
creator: Zhenrun ZHANG
created: 2011-09-27T11:00:00Z
modified: 2015-05-08T01:12:50Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _770_
m user id: 0
owner id: 8606
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: B0015+pBAD+vioAB+B0015+pBAD+TPP ribozyme 1.20+vioD+B0015+pBAD+vioE
mutable notes: The Ribosomal Binding Site of vioD is contained in TPP ribozyme 1.20.
mutable provenance: The hammerhead ribozyme comes from E. coli, and the TPP aptamer comes from B. subtilis.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: vioB
sequence annotation: pBAD
sequence annotation: TPP ribozyme 1.20
sequence annotation: rbs
sequence annotation: vioD
sequence annotation: vioE
sequence annotation: B0015
sequence annotation: vioA
sequence: https://synbiohub.org/public/igem/BBa_K598020_sequence/1

vioB
type: SequenceAnnotation
persistent identity: annotation2154542
display id: annotation2154542
version: 1
top level: BBa_K598020
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K598020/annotation2154542/range2154542/1

https://synbiohub.org/public/igem/BBa_K598020/annotation2154542/range2154542/1
type: Range
persistent identity: range2154542
display id: range2154542
version: 1
direction: direction/forward
top level: BBa_K598020
start: 1559
end: 4556
orientation: inline

pBAD
type: SequenceAnnotation
persistent identity: annotation2154515
display id: annotation2154515
version: 1
top level: BBa_K598020
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K598020/annotation2154515/range2154515/1

https://synbiohub.org/public/igem/BBa_K598020/annotation2154515/range2154515/1
type: Range
persistent identity: range2154515
display id: range2154515
version: 1
direction: direction/forward
top level: BBa_K598020
start: 138
end: 267
orientation: inline

TPP ribozyme 1.20
type: SequenceAnnotation
persistent identity: annotation2154556
display id: annotation2154556
version: 1
top level: BBa_K598020
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K598020/annotation2154556/range2154556/1

https://synbiohub.org/public/igem/BBa_K598020/annotation2154556/range2154556/1
type: Range
persistent identity: range2154556
display id: range2154556
version: 1
direction: direction/forward
top level: BBa_K598020
start: 4838
end: 4987
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2154516
display id: annotation2154516
version: 1
top level: BBa_K598020
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K598020/annotation2154516/range2154516/1

https://synbiohub.org/public/igem/BBa_K598020/annotation2154516/range2154516/1
type: Range
persistent identity: range2154516
display id: range2154516
version: 1
direction: direction/forward
top level: BBa_K598020
start: 273
end: 279
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2154575
display id: annotation2154575
version: 1
top level: BBa_K598020
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K598020/annotation2154575/range2154575/1

https://synbiohub.org/public/igem/BBa_K598020/annotation2154575/range2154575/1
type: Range
persistent identity: range2154575
display id: range2154575
version: 1
direction: direction/forward
top level: BBa_K598020
start: 4976
end: 4982
orientation: inline

vioD
type: SequenceAnnotation
persistent identity: annotation2154576
display id: annotation2154576
version: 1
top level: BBa_K598020
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K598020/annotation2154576/range2154576/1

https://synbiohub.org/public/igem/BBa_K598020/annotation2154576/range2154576/1
type: Range
persistent identity: range2154576
display id: range2154576
version: 1
direction: direction/forward
top level: BBa_K598020
start: 4988
end: 6112
orientation: inline

vioE
type: SequenceAnnotation
persistent identity: annotation2154586
display id: annotation2154586
version: 1
top level: BBa_K598020
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K598020/annotation2154586/range2154586/1

https://synbiohub.org/public/igem/BBa_K598020/annotation2154586/range2154586/1
type: Range
persistent identity: range2154586
display id: range2154586
version: 1
direction: direction/forward
top level: BBa_K598020
start: 6489
end: 7064
orientation: inline

pBAD
type: SequenceAnnotation
persistent identity: annotation2154548
display id: annotation2154548
version: 1
top level: BBa_K598020
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K598020/annotation2154548/range2154548/1

https://synbiohub.org/public/igem/BBa_K598020/annotation2154548/range2154548/1
type: Range
persistent identity: range2154548
display id: range2154548
version: 1
direction: direction/forward
top level: BBa_K598020
start: 4702
end: 4831
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2154545
display id: annotation2154545
version: 1
top level: BBa_K598020
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K598020/annotation2154545/range2154545/1

https://synbiohub.org/public/igem/BBa_K598020/annotation2154545/range2154545/1
type: Range
persistent identity: range2154545
display id: range2154545
version: 1
direction: direction/forward
top level: BBa_K598020
start: 4565
end: 4693
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2154514
display id: annotation2154514
version: 1
top level: BBa_K598020
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K598020/annotation2154514/range2154514/1

https://synbiohub.org/public/igem/BBa_K598020/annotation2154514/range2154514/1
type: Range
persistent identity: range2154514
display id: range2154514
version: 1
direction: direction/forward
top level: BBa_K598020
start: 1
end: 129
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2154518
display id: annotation2154518
version: 1
top level: BBa_K598020
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K598020/annotation2154518/range2154518/1

https://synbiohub.org/public/igem/BBa_K598020/annotation2154518/range2154518/1
type: Range
persistent identity: range2154518
display id: range2154518
version: 1
direction: direction/forward
top level: BBa_K598020
start: 1545
end: 1551
orientation: inline

vioA
type: SequenceAnnotation
persistent identity: annotation2154517
display id: annotation2154517
version: 1
top level: BBa_K598020
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K598020/annotation2154517/range2154517/1

https://synbiohub.org/public/igem/BBa_K598020/annotation2154517/range2154517/1
type: Range
persistent identity: range2154517
display id: range2154517
version: 1
direction: direction/forward
top level: BBa_K598020
start: 285
end: 1542
orientation: inline

pBAD
type: SequenceAnnotation
persistent identity: annotation2154577
display id: annotation2154577
version: 1
top level: BBa_K598020
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K598020/annotation2154577/range2154577/1

https://synbiohub.org/public/igem/BBa_K598020/annotation2154577/range2154577/1
type: Range
persistent identity: range2154577
display id: range2154577
version: 1
direction: direction/forward
top level: BBa_K598020
start: 6342
end: 6471
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2154583
display id: annotation2154583
version: 1
top level: BBa_K598020
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K598020/annotation2154583/range2154583/1

https://synbiohub.org/public/igem/BBa_K598020/annotation2154583/range2154583/1
type: Range
persistent identity: range2154583
display id: range2154583
version: 1
direction: direction/forward
top level: BBa_K598020
start: 6205
end: 6333
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2154588
display id: annotation2154588
version: 1
top level: BBa_K598020
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K598020/annotation2154588/range2154588/1

https://synbiohub.org/public/igem/BBa_K598020/annotation2154588/range2154588/1
type: Range
persistent identity: range2154588
display id: range2154588
version: 1
direction: direction/forward
top level: BBa_K598020
start: 6477
end: 6483
orientation: inline

BBa_S03518
type: ComponentDefinition
persistent identity: BBa S03518
display id: BBa_S03518
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0034:C0040
creator: Azeem Kaka
created: 2006-07-23T11:00:00Z
modified: 2015-05-08T01:14:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _58_
m user id: 0
owner id: 1061
owning group id: 58
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_C0040
component: BBa_B0034
sequence annotation: BBa_C0040
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_S03518_sequence/1

BBa_C0040
type: Component
persistent identity: component2243685
display id: component2243685
version: 1
top level: BBa_S03518
access: public
definition: tetR

BBa_B0034
type: Component
persistent identity: component2243681
display id: component2243681
version: 1
top level: BBa_S03518
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation2243685
display id: annotation2243685
version: 1
top level: BBa_S03518
location: https://synbiohub.org/public/igem/BBa_S03518/annotation2243685/range2243685/1
component: BBa_C0040

https://synbiohub.org/public/igem/BBa_S03518/annotation2243685/range2243685/1
type: Range
persistent identity: range2243685
display id: range2243685
version: 1
direction: direction/reverse
top level: BBa_S03518
start: 19
end: 703
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2243681
display id: annotation2243681
version: 1
top level: BBa_S03518
location: https://synbiohub.org/public/igem/BBa_S03518/annotation2243681/range2243681/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_S03518/annotation2243681/range2243681/1
type: Range
persistent identity: range2243681
display id: range2243681
version: 1
direction: direction/reverse
top level: BBa_S03518
start: 1
end: 12
orientation: inline

cinR
type: ComponentDefinition
persistent identity: BBa R0078
display id: BBa_R0078
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter (cinR and HSL regulated)
creator: Drew Endy
created: 2004-01-29T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable provenance: Rhizobium leguminosarum
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: stem_loop
sequence annotation: unidentified/uncharacterized CinR dependent
sequence: https://synbiohub.org/public/igem/BBa_R0078_sequence/1

stem_loop
type: SequenceAnnotation
persistent identity: annotation318244
display id: annotation318244
version: 1
top level: cinR
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_R0078/annotation318244/range318244/1

https://synbiohub.org/public/igem/BBa_R0078/annotation318244/range318244/1
type: Range
persistent identity: range318244
display id: range318244
version: 1
direction: direction/forward
top level: cinR
start: 35
end: 74
orientation: inline

unidentified/uncharacterized CinR dependent
type: SequenceAnnotation
persistent identity: annotation318234
display id: annotation318234
version: 1
top level: cinR
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_R0078/annotation318234/range318234/1

https://synbiohub.org/public/igem/BBa_R0078/annotation318234/range318234/1
type: Range
persistent identity: range318234
display id: range318234
version: 1
direction: direction/forward
top level: cinR
start: 1
end: 220
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation318250
display id: annotation318250
version: 1
top level: cinR
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_R0078/annotation318250/range318250/1

https://synbiohub.org/public/igem/BBa_R0078/annotation318250/range318250/1
type: Range
persistent identity: range318250
display id: range318250
version: 1
direction: direction/forward
top level: cinR
start: 91
end: 136
orientation: inline

BBa_K598019
type: ComponentDefinition
persistent identity: BBa K598019
display id: BBa_K598019
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0015+pBAD+vioABD+B0015+pBAD+TPP Down-regulated Hammerhead Ribozyme 2.5+vioE
creator: Zhenrun ZHANG
created: 2011-09-26T11:00:00Z
modified: 2015-05-08T01:12:50Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _770_
m user id: 0
owner id: 8606
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: B0015+pBAD+vioABD+B0015+pBAD+TPP Down-regulated Hammerhead Ribozyme 2.5+vioE
mutable notes: the Ribosomal Binding Site of vioE is contained in TPP Ribozyme.
mutable provenance: Hammerhead Ribozyme backbone comes from E.coli, and TPP aptamer comes from B.subtilis.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: TPP ribozyme 2.5
sequence annotation: vioB
sequence annotation: vioA
sequence annotation: B0015
sequence annotation: pBAD
sequence annotation: vioE
sequence annotation: rbs
sequence annotation: vioD
sequence: https://synbiohub.org/public/igem/BBa_K598019_sequence/1

TPP ribozyme 2.5
type: SequenceAnnotation
persistent identity: annotation2144624
display id: annotation2144624
version: 1
top level: BBa_K598019
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K598019/annotation2144624/range2144624/1

https://synbiohub.org/public/igem/BBa_K598019/annotation2144624/range2144624/1
type: Range
persistent identity: range2144624
display id: range2144624
version: 1
direction: direction/forward
top level: BBa_K598019
start: 5985
end: 6134
orientation: inline

vioB
type: SequenceAnnotation
persistent identity: annotation2144599
display id: annotation2144599
version: 1
top level: BBa_K598019
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K598019/annotation2144599/range2144599/1

https://synbiohub.org/public/igem/BBa_K598019/annotation2144599/range2144599/1
type: Range
persistent identity: range2144599
display id: range2144599
version: 1
direction: direction/forward
top level: BBa_K598019
start: 1559
end: 4556
orientation: inline

vioA
type: SequenceAnnotation
persistent identity: annotation2144597
display id: annotation2144597
version: 1
top level: BBa_K598019
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K598019/annotation2144597/range2144597/1

https://synbiohub.org/public/igem/BBa_K598019/annotation2144597/range2144597/1
type: Range
persistent identity: range2144597
display id: range2144597
version: 1
direction: direction/forward
top level: BBa_K598019
start: 285
end: 1542
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2144593
display id: annotation2144593
version: 1
top level: BBa_K598019
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K598019/annotation2144593/range2144593/1

https://synbiohub.org/public/igem/BBa_K598019/annotation2144593/range2144593/1
type: Range
persistent identity: range2144593
display id: range2144593
version: 1
direction: direction/forward
top level: BBa_K598019
start: 1
end: 129
orientation: inline

pBAD
type: SequenceAnnotation
persistent identity: annotation2144602
display id: annotation2144602
version: 1
top level: BBa_K598019
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K598019/annotation2144602/range2144602/1

https://synbiohub.org/public/igem/BBa_K598019/annotation2144602/range2144602/1
type: Range
persistent identity: range2144602
display id: range2144602
version: 1
direction: direction/forward
top level: BBa_K598019
start: 5849
end: 5978
orientation: inline

pBAD
type: SequenceAnnotation
persistent identity: annotation2144595
display id: annotation2144595
version: 1
top level: BBa_K598019
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K598019/annotation2144595/range2144595/1

https://synbiohub.org/public/igem/BBa_K598019/annotation2144595/range2144595/1
type: Range
persistent identity: range2144595
display id: range2144595
version: 1
direction: direction/forward
top level: BBa_K598019
start: 138
end: 267
orientation: inline

vioE
type: SequenceAnnotation
persistent identity: annotation2144631
display id: annotation2144631
version: 1
top level: BBa_K598019
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K598019/annotation2144631/range2144631/1

https://synbiohub.org/public/igem/BBa_K598019/annotation2144631/range2144631/1
type: Range
persistent identity: range2144631
display id: range2144631
version: 1
direction: direction/forward
top level: BBa_K598019
start: 6135
end: 6710
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2144598
display id: annotation2144598
version: 1
top level: BBa_K598019
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K598019/annotation2144598/range2144598/1

https://synbiohub.org/public/igem/BBa_K598019/annotation2144598/range2144598/1
type: Range
persistent identity: range2144598
display id: range2144598
version: 1
direction: direction/forward
top level: BBa_K598019
start: 1545
end: 1551
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2144596
display id: annotation2144596
version: 1
top level: BBa_K598019
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K598019/annotation2144596/range2144596/1

https://synbiohub.org/public/igem/BBa_K598019/annotation2144596/range2144596/1
type: Range
persistent identity: range2144596
display id: range2144596
version: 1
direction: direction/forward
top level: BBa_K598019
start: 273
end: 279
orientation: inline

vioD
type: SequenceAnnotation
persistent identity: annotation2144600
display id: annotation2144600
version: 1
top level: BBa_K598019
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K598019/annotation2144600/range2144600/1

https://synbiohub.org/public/igem/BBa_K598019/annotation2144600/range2144600/1
type: Range
persistent identity: range2144600
display id: range2144600
version: 1
direction: direction/forward
top level: BBa_K598019
start: 4579
end: 5704
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2144601
display id: annotation2144601
version: 1
top level: BBa_K598019
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K598019/annotation2144601/range2144601/1

https://synbiohub.org/public/igem/BBa_K598019/annotation2144601/range2144601/1
type: Range
persistent identity: range2144601
display id: range2144601
version: 1
direction: direction/forward
top level: BBa_K598019
start: 5712
end: 5841
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2144594
display id: annotation2144594
version: 1
top level: BBa_K598019
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K598019/annotation2144594/range2144594/1

https://synbiohub.org/public/igem/BBa_K598019/annotation2144594/range2144594/1
type: Range
persistent identity: range2144594
display id: range2144594
version: 1
direction: direction/forward
top level: BBa_K598019
start: 4565
end: 4571
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2144630
display id: annotation2144630
version: 1
top level: BBa_K598019
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K598019/annotation2144630/range2144630/1

https://synbiohub.org/public/igem/BBa_K598019/annotation2144630/range2144630/1
type: Range
persistent identity: range2144630
display id: range2144630
version: 1
direction: direction/forward
top level: BBa_K598019
start: 6122
end: 6129
orientation: inline

BBa_I718012
type: ComponentDefinition
persistent identity: BBa I718012
display id: BBa_I718012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ptet-lox71-ter
creator: David Bikard
created: 2007-10-25T11:00:00Z
modified: 2015-08-31T04:07:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _141_
m user id: 0
owner id: 1481
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Upstream construct of the Synthetics Multicellular Bacterium
mutable notes: none
mutable provenance: none
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_B0010
component: BBa_I718017
component: BBa_R0040
component: BBa_B0012
sequence annotation: BBa_R0040
sequence annotation: BBa_B0012
sequence annotation: BBa_I718017
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_I718012_sequence/1

BBa_B0010
type: Component
persistent identity: component1956342
display id: component1956342
version: 1
top level: BBa_I718012
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_I718017
type: Component
persistent identity: component1956341
display id: component1956341
version: 1
top level: BBa_I718012
access: public
definition: https://synbiohub.org/public/igem/BBa_I718017/1

BBa_R0040
type: Component
persistent identity: component1956337
display id: component1956337
version: 1
top level: BBa_I718012
access: public
definition: p(tetR)

BBa_B0012
type: Component
persistent identity: component1956344
display id: component1956344
version: 1
top level: BBa_I718012
access: public
definition: BBa_B0012

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1956337
display id: annotation1956337
version: 1
top level: BBa_I718012
location: https://synbiohub.org/public/igem/BBa_I718012/annotation1956337/range1956337/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_I718012/annotation1956337/range1956337/1
type: Range
persistent identity: range1956337
display id: range1956337
version: 1
direction: direction/forward
top level: BBa_I718012
start: 1
end: 54
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1956344
display id: annotation1956344
version: 1
top level: BBa_I718012
location: https://synbiohub.org/public/igem/BBa_I718012/annotation1956344/range1956344/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I718012/annotation1956344/range1956344/1
type: Range
persistent identity: range1956344
display id: range1956344
version: 1
direction: direction/forward
top level: BBa_I718012
start: 193
end: 233
orientation: inline

BBa_I718017
type: SequenceAnnotation
persistent identity: annotation1956341
display id: annotation1956341
version: 1
top level: BBa_I718012
location: https://synbiohub.org/public/igem/BBa_I718012/annotation1956341/range1956341/1
component: BBa_I718017

https://synbiohub.org/public/igem/BBa_I718012/annotation1956341/range1956341/1
type: Range
persistent identity: range1956341
display id: range1956341
version: 1
direction: direction/reverse
top level: BBa_I718012
start: 63
end: 96
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1956342
display id: annotation1956342
version: 1
top level: BBa_I718012
location: https://synbiohub.org/public/igem/BBa_I718012/annotation1956342/range1956342/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I718012/annotation1956342/range1956342/1
type: Range
persistent identity: range1956342
display id: range1956342
version: 1
direction: direction/forward
top level: BBa_I718012
start: 105
end: 184
orientation: inline

BBa_K733002
type: ComponentDefinition
persistent identity: BBa K733002
display id: BBa_K733002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: xylR+PxylA: A xylose inducible promoter with its transcriptional regulator.
creator: WANG, Yuqi
created: 2012-09-15T11:00:00Z
modified: 2015-05-08T01:13:06Z
discontinued: false
dominant: false
experience: experience/None
group u list: _979_
m user id: 0
owner id: 12444
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Not available at this moment.
mutable notes: Not available at this moment.
mutable provenance: Not available at this moment.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Sigma A, -35
sequence annotation: xylR
sequence annotation: Sigma A, -10
sequence: https://synbiohub.org/public/igem/BBa_K733002_sequence/1

Sigma A, -35
type: SequenceAnnotation
persistent identity: annotation2193604
display id: annotation2193604
version: 1
top level: BBa_K733002
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K733002/annotation2193604/range2193604/1

https://synbiohub.org/public/igem/BBa_K733002/annotation2193604/range2193604/1
type: Range
persistent identity: range2193604
display id: range2193604
version: 1
direction: direction/forward
top level: BBa_K733002
start: 1277
end: 1282
orientation: inline

xylR
type: SequenceAnnotation
persistent identity: annotation2193589
display id: annotation2193589
version: 1
top level: BBa_K733002
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K733002/annotation2193589/range2193589/1

https://synbiohub.org/public/igem/BBa_K733002/annotation2193589/range2193589/1
type: Range
persistent identity: range2193589
display id: range2193589
version: 1
direction: direction/reverse
top level: BBa_K733002
start: 7
end: 1173
orientation: inline

Sigma A, -10
type: SequenceAnnotation
persistent identity: annotation2193603
display id: annotation2193603
version: 1
top level: BBa_K733002
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K733002/annotation2193603/range2193603/1

https://synbiohub.org/public/igem/BBa_K733002/annotation2193603/range2193603/1
type: Range
persistent identity: range2193603
display id: range2193603
version: 1
direction: direction/forward
top level: BBa_K733002
start: 1300
end: 1305
orientation: inline

BBa_K519030
type: ComponentDefinition
persistent identity: BBa K519030
display id: BBa_K519030
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Photocontrol device
creator: Chifuku Mita
created: 2011-09-27T11:00:00Z
modified: 2015-05-08T01:12:33Z
discontinued: false
dominant: false
experience: experience/None
group u list: _684_
m user id: 0
owner id: 9626
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: a
mutable notes: a
mutable provenance: a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_I15010
component: BBa_I15008
component: BBa_I15009
component: BBa_B0034
component: BBa_B0015
sequence annotation: BBa_B0034
sequence annotation: BBa_B0015
sequence annotation: BBa_I15008
sequence annotation: BBa_I15009
sequence annotation: BBa_I15010
sequence: https://synbiohub.org/public/igem/BBa_K519030_sequence/1

BBa_I15010
type: Component
persistent identity: component2273724
display id: component2273724
version: 1
top level: BBa_K519030
access: public
definition: https://synbiohub.org/public/igem/BBa_I15010/1

BBa_I15008
type: Component
persistent identity: component2273714
display id: component2273714
version: 1
top level: BBa_K519030
access: public
definition: https://synbiohub.org/public/igem/BBa_I15008/1

BBa_I15009
type: Component
persistent identity: component2273719
display id: component2273719
version: 1
top level: BBa_K519030
access: public
definition: https://synbiohub.org/public/igem/BBa_I15009/1

BBa_B0034
type: Component
persistent identity: component2273712
display id: component2273712
version: 1
top level: BBa_K519030
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: Component
persistent identity: component2273716
display id: component2273716
version: 1
top level: BBa_K519030
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0015
type: Component
persistent identity: component2273731
display id: component2273731
version: 1
top level: BBa_K519030
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_B0034
type: Component
persistent identity: component2273721
display id: component2273721
version: 1
top level: BBa_K519030
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2273716
display id: annotation2273716
version: 1
top level: BBa_K519030
location: https://synbiohub.org/public/igem/BBa_K519030/annotation2273716/range2273716/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K519030/annotation2273716/range2273716/1
type: Range
persistent identity: range2273716
display id: range2273716
version: 1
direction: direction/reverse
top level: BBa_K519030
start: 778
end: 789
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2273731
display id: annotation2273731
version: 1
top level: BBa_K519030
location: https://synbiohub.org/public/igem/BBa_K519030/annotation2273731/range2273731/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K519030/annotation2273731/range2273731/1
type: Range
persistent identity: range2273731
display id: range2273731
version: 1
direction: direction/reverse
top level: BBa_K519030
start: 3868
end: 3996
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2273712
display id: annotation2273712
version: 1
top level: BBa_K519030
location: https://synbiohub.org/public/igem/BBa_K519030/annotation2273712/range2273712/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K519030/annotation2273712/range2273712/1
type: Range
persistent identity: range2273712
display id: range2273712
version: 1
direction: direction/reverse
top level: BBa_K519030
start: 1
end: 12
orientation: inline

BBa_I15008
type: SequenceAnnotation
persistent identity: annotation2273714
display id: annotation2273714
version: 1
top level: BBa_K519030
location: https://synbiohub.org/public/igem/BBa_K519030/annotation2273714/range2273714/1
component: BBa_I15008

https://synbiohub.org/public/igem/BBa_K519030/annotation2273714/range2273714/1
type: Range
persistent identity: range2273714
display id: range2273714
version: 1
direction: direction/reverse
top level: BBa_K519030
start: 19
end: 769
orientation: inline

BBa_I15009
type: SequenceAnnotation
persistent identity: annotation2273719
display id: annotation2273719
version: 1
top level: BBa_K519030
location: https://synbiohub.org/public/igem/BBa_K519030/annotation2273719/range2273719/1
component: BBa_I15009

https://synbiohub.org/public/igem/BBa_K519030/annotation2273719/range2273719/1
type: Range
persistent identity: range2273719
display id: range2273719
version: 1
direction: direction/reverse
top level: BBa_K519030
start: 796
end: 1570
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2273721
display id: annotation2273721
version: 1
top level: BBa_K519030
location: https://synbiohub.org/public/igem/BBa_K519030/annotation2273721/range2273721/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K519030/annotation2273721/range2273721/1
type: Range
persistent identity: range2273721
display id: range2273721
version: 1
direction: direction/reverse
top level: BBa_K519030
start: 1579
end: 1590
orientation: inline

BBa_I15010
type: SequenceAnnotation
persistent identity: annotation2273724
display id: annotation2273724
version: 1
top level: BBa_K519030
location: https://synbiohub.org/public/igem/BBa_K519030/annotation2273724/range2273724/1
component: BBa_I15010

https://synbiohub.org/public/igem/BBa_K519030/annotation2273724/range2273724/1
type: Range
persistent identity: range2273724
display id: range2273724
version: 1
direction: direction/reverse
top level: BBa_K519030
start: 1597
end: 3859
orientation: inline

BBa_I715032
type: ComponentDefinition
persistent identity: BBa I715032
display id: BBa_I715032
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: lacIq reverse
creator: Jordan Baumgardner
created: 2007-07-17T11:00:00Z
modified: 2015-08-31T04:07:49Z
discontinued: false
dominant: false
experience: experience/None
group u list: _120_
m user id: 0
owner id: 1518
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is a reverse version of BBa_C0012, the lacIq repressor cds.
mutable notes: Standard biobrick prefix and suffix were used. Since PCR was used, DNA sequence verification was needed - no mutations were found.
mutable provenance: PCR was used to amplify C0012 using primers that switched the biobrick prefix and suffix.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_I715032_sequence/1

BBa_B0031
type: ComponentDefinition
persistent identity: BBa B0031
display id: BBa_B0031
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS.2 (weak) -- derivative of BBa_0030
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Medium RBS based on Ron Weiss thesis. Strength considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-1" in figure 4-14 of thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Cho
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0031_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23316
display id: annotation23316
version: 1
top level: BBa_B0031
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_B0031/annotation23316/range23316/1

https://synbiohub.org/public/igem/BBa_B0031/annotation23316/range23316/1
type: Range
persistent identity: range23316
display id: range23316
version: 1
direction: direction/forward
top level: BBa_B0031
start: 7
end: 10
orientation: inline

BBa_K786002
type: ComponentDefinition
persistent identity: BBa K786002
display id: BBa_K786002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Sensory rhodopsin II (SRII) with HtrII & Tar, sensitive to blue-green light
creator: Leung Wai Tak
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:22Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1041_
m user id: 0
owner id: 14285
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Sensory rhodopsin II fusion with HtrII & Tar, which is sensitive to blue-green light, with sensing spectra covering from 400-500 nm. We used it for sensing blur-green light to achieve phototaxis and switch for downstream gene expression.
mutable notes: Pfam (version 26.0) was used to predict the domain of fusion protein. Linker was refereed and modified from previous research study of SRII fusion with HtrII.
mutable provenance: From genomic sequence of bacterial N. pharaonis (DSM 2160) and E.coli K12.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: BamHI for C-terminal fusion
sequence annotation: SRII-HtrII-Tar
sequence annotation: HindIII for N-terminal fusin
sequence annotation: B0015
sequence annotation: Constitutive promoter BBa_J23100
sequence annotation: RBS 1.0
sequence: https://synbiohub.org/public/igem/BBa_K786002_sequence/1

BamHI for C-terminal fusion
type: SequenceAnnotation
persistent identity: annotation2199693
display id: annotation2199693
version: 1
top level: BBa_K786002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K786002/annotation2199693/range2199693/1

https://synbiohub.org/public/igem/BBa_K786002/annotation2199693/range2199693/1
type: Range
persistent identity: range2199693
display id: range2199693
version: 1
direction: direction/forward
top level: BBa_K786002
start: 785
end: 790
orientation: inline

SRII-HtrII-Tar
type: SequenceAnnotation
persistent identity: annotation2199692
display id: annotation2199692
version: 1
top level: BBa_K786002
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K786002/annotation2199692/range2199692/1

https://synbiohub.org/public/igem/BBa_K786002/annotation2199692/range2199692/1
type: Range
persistent identity: range2199692
display id: range2199692
version: 1
direction: direction/forward
top level: BBa_K786002
start: 68
end: 2083
orientation: inline

HindIII for N-terminal fusin
type: SequenceAnnotation
persistent identity: annotation2199691
display id: annotation2199691
version: 1
top level: BBa_K786002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K786002/annotation2199691/range2199691/1

https://synbiohub.org/public/igem/BBa_K786002/annotation2199691/range2199691/1
type: Range
persistent identity: range2199691
display id: range2199691
version: 1
direction: direction/forward
top level: BBa_K786002
start: 62
end: 67
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2199694
display id: annotation2199694
version: 1
top level: BBa_K786002
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K786002/annotation2199694/range2199694/1

https://synbiohub.org/public/igem/BBa_K786002/annotation2199694/range2199694/1
type: Range
persistent identity: range2199694
display id: range2199694
version: 1
direction: direction/forward
top level: BBa_K786002
start: 2117
end: 2245
orientation: inline

Constitutive promoter BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2199689
display id: annotation2199689
version: 1
top level: BBa_K786002
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K786002/annotation2199689/range2199689/1

https://synbiohub.org/public/igem/BBa_K786002/annotation2199689/range2199689/1
type: Range
persistent identity: range2199689
display id: range2199689
version: 1
direction: direction/forward
top level: BBa_K786002
start: 1
end: 41
orientation: inline

RBS 1.0
type: SequenceAnnotation
persistent identity: annotation2199690
display id: annotation2199690
version: 1
top level: BBa_K786002
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K786002/annotation2199690/range2199690/1

https://synbiohub.org/public/igem/BBa_K786002/annotation2199690/range2199690/1
type: Range
persistent identity: range2199690
display id: range2199690
version: 1
direction: direction/forward
top level: BBa_K786002
start: 42
end: 61
orientation: inline

BBa_K779503
type: ComponentDefinition
persistent identity: BBa K779503
display id: BBa_K779503
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Long input strand MammoBlock
creator: Kristjan Eerik Kaseniit
created: 2012-09-28T11:00:00Z
modified: 2015-05-08T01:13:18Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1033_
m user id: 0
owner id: 12684
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Long matching input strand to the reporter formed by parts Part:BBa K779500 and Part:BBa K779502 or Part:BBa K779501 and Part:BBa K779502. Modifications: 5' IRD800 fluorophore, 3' phosphate. IDT DNA oligo order: /5IRD800/mCmA mCmCmC mAmCmU mCmCmU mCmUmC mCmCmA mCmCmA mAmCmU mAmUmC mCmA/3Phos/
mutable notes: 3' phosphate added to protect from some 3' exonucleases (suggested by IDT). See more details at the MIT iGEM wiki: http://2012.igem.org/Team:MIT
mutable provenance: De novo design.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence annotation: Toehold
sequence annotation: Domain Sa
sequence: https://synbiohub.org/public/igem/BBa_K779503_sequence/1

Toehold
type: SequenceAnnotation
persistent identity: annotation2204256
display id: annotation2204256
version: 1
top level: BBa_K779503
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K779503/annotation2204256/range2204256/1

https://synbiohub.org/public/igem/BBa_K779503/annotation2204256/range2204256/1
type: Range
persistent identity: range2204256
display id: range2204256
version: 1
direction: direction/forward
top level: BBa_K779503
start: 21
end: 28
orientation: inline

Domain Sa
type: SequenceAnnotation
persistent identity: annotation2204255
display id: annotation2204255
version: 1
top level: BBa_K779503
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K779503/annotation2204255/range2204255/1

https://synbiohub.org/public/igem/BBa_K779503/annotation2204255/range2204255/1
type: Range
persistent identity: range2204255
display id: range2204255
version: 1
direction: direction/forward
top level: BBa_K779503
start: 1
end: 20
orientation: inline

BBa_K876004
type: ComponentDefinition
persistent identity: BBa K876004
display id: BBa_K876004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pTet-RBS-GFP-T-pLac-TetR
creator: Alec Spinhirne
created: 2012-09-29T11:00:00Z
modified: 2015-05-08T01:13:38Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1138_
m user id: 0
owner id: 13834
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Test mechanism for the effectiveness of TetR in repressing pTet. Also used to show the effect of ATc on TetR
mutable notes: Biobrick placement
mutable provenance: Created from biobricks
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K081012
component: BBa_R0010
component: BBa_C0040
component: BBa_R0040
sequence annotation: BBa_R0040
sequence annotation: BBa_R0010
sequence annotation: BBa_C0040
sequence annotation: BBa_K081012
sequence: https://synbiohub.org/public/igem/BBa_K876004_sequence/1

BBa_K081012
type: Component
persistent identity: component2264640
display id: component2264640
version: 1
top level: BBa_K876004
access: public
definition: https://synbiohub.org/public/igem/BBa_K081012/1

BBa_R0010
type: Component
persistent identity: component2264641
display id: component2264641
version: 1
top level: BBa_K876004
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_C0040
type: Component
persistent identity: component2264651
display id: component2264651
version: 1
top level: BBa_K876004
access: public
definition: tetR

BBa_R0040
type: Component
persistent identity: component2264625
display id: component2264625
version: 1
top level: BBa_K876004
access: public
definition: p(tetR)

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2264625
display id: annotation2264625
version: 1
top level: BBa_K876004
location: https://synbiohub.org/public/igem/BBa_K876004/annotation2264625/range2264625/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K876004/annotation2264625/range2264625/1
type: Range
persistent identity: range2264625
display id: range2264625
version: 1
direction: direction/forward
top level: BBa_K876004
start: 1
end: 54
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2264641
display id: annotation2264641
version: 1
top level: BBa_K876004
location: https://synbiohub.org/public/igem/BBa_K876004/annotation2264641/range2264641/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K876004/annotation2264641/range2264641/1
type: Range
persistent identity: range2264641
display id: range2264641
version: 1
direction: direction/forward
top level: BBa_K876004
start: 859
end: 1058
orientation: inline

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation2264651
display id: annotation2264651
version: 1
top level: BBa_K876004
location: https://synbiohub.org/public/igem/BBa_K876004/annotation2264651/range2264651/1
component: BBa_C0040

https://synbiohub.org/public/igem/BBa_K876004/annotation2264651/range2264651/1
type: Range
persistent identity: range2264651
display id: range2264651
version: 1
direction: direction/reverse
top level: BBa_K876004
start: 1065
end: 1749
orientation: inline

BBa_K081012
type: SequenceAnnotation
persistent identity: annotation2264640
display id: annotation2264640
version: 1
top level: BBa_K876004
location: https://synbiohub.org/public/igem/BBa_K876004/annotation2264640/range2264640/1
component: BBa_K081012

https://synbiohub.org/public/igem/BBa_K876004/annotation2264640/range2264640/1
type: Range
persistent identity: range2264640
display id: range2264640
version: 1
direction: direction/reverse
top level: BBa_K876004
start: 63
end: 850
orientation: inline

BBa_I746320
type: ComponentDefinition
persistent identity: BBa I746320
display id: BBa_I746320
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP under control of PF promoter
creator: Stefan Milde
created: 2007-09-10T11:00:00Z
modified: 2015-08-31T04:08:04Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _116_
m user id: 0
owner id: 2122
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part is used to screen the level of induction of the PF promoter (I746360). The PF promoter is induced by a class of phage activators (P2 ogr: I746350, PSP3pag: I746351, phiR73 delta: I746352).
mutable notes: The PF promoter was put upstream of I13504.
mutable provenance: registry (I13504) and own construct (I746360)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0012
component: BBa_I746360
component: BBa_E0040
component: BBa_B0034
component: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_E0040
sequence annotation: BBa_B0034
sequence annotation: BBa_I746360
sequence: https://synbiohub.org/public/igem/BBa_I746320_sequence/1

BBa_B0012
type: Component
persistent identity: component1943797
display id: component1943797
version: 1
top level: BBa_I746320
access: public
definition: BBa_B0012

BBa_I746360
type: Component
persistent identity: component1943790
display id: component1943790
version: 1
top level: BBa_I746320
access: public
definition: https://synbiohub.org/public/igem/BBa_I746360/1

BBa_E0040
type: Component
persistent identity: component1943794
display id: component1943794
version: 1
top level: BBa_I746320
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_B0034
type: Component
persistent identity: component1943792
display id: component1943792
version: 1
top level: BBa_I746320
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component1943795
display id: component1943795
version: 1
top level: BBa_I746320
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1943797
display id: annotation1943797
version: 1
top level: BBa_I746320
location: https://synbiohub.org/public/igem/BBa_I746320/annotation1943797/range1943797/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I746320/annotation1943797/range1943797/1
type: Range
persistent identity: range1943797
display id: range1943797
version: 1
direction: direction/forward
top level: BBa_I746320
start: 934
end: 974
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1943795
display id: annotation1943795
version: 1
top level: BBa_I746320
location: https://synbiohub.org/public/igem/BBa_I746320/annotation1943795/range1943795/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I746320/annotation1943795/range1943795/1
type: Range
persistent identity: range1943795
display id: range1943795
version: 1
direction: direction/forward
top level: BBa_I746320
start: 846
end: 925
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1943794
display id: annotation1943794
version: 1
top level: BBa_I746320
location: https://synbiohub.org/public/igem/BBa_I746320/annotation1943794/range1943794/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_I746320/annotation1943794/range1943794/1
type: Range
persistent identity: range1943794
display id: range1943794
version: 1
direction: direction/reverse
top level: BBa_I746320
start: 118
end: 837
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1943792
display id: annotation1943792
version: 1
top level: BBa_I746320
location: https://synbiohub.org/public/igem/BBa_I746320/annotation1943792/range1943792/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I746320/annotation1943792/range1943792/1
type: Range
persistent identity: range1943792
display id: range1943792
version: 1
direction: direction/reverse
top level: BBa_I746320
start: 100
end: 111
orientation: inline

BBa_I746360
type: SequenceAnnotation
persistent identity: annotation1943790
display id: annotation1943790
version: 1
top level: BBa_I746320
location: https://synbiohub.org/public/igem/BBa_I746320/annotation1943790/range1943790/1
component: BBa_I746360

https://synbiohub.org/public/igem/BBa_I746320/annotation1943790/range1943790/1
type: Range
persistent identity: range1943790
display id: range1943790
version: 1
direction: direction/reverse
top level: BBa_I746320
start: 1
end: 91
orientation: inline

BBa_K880002
type: ComponentDefinition
persistent identity: BBa K880002
display id: BBa_K880002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP fluorescence reporter to assay the activity of FimE K137007 and HbiF K880000 recombinases.
creator: Josh Atkinson, Mike Ferguson, and Ben Parker
created: 2012-09-28T11:00:00Z
modified: 2015-05-08T01:13:40Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1142_
m user id: 0
owner id: 9403
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -IRR K137008 + inverted tetR promoter K137047 + IRL K137010 + GFP I13504 (MI275) -GFP fluorescence reporter to assay the activity of FimE K137007 and HbiF K880000 recombinases. Same components as K137058 with the inverted repeats reversed such that the external half sites are external to the flip region (IRR - ???flip region??? - IRL) GFP fluorescent reporter capable of assaying the functionality of fim regulatory recombinases; based on K137058. K137058???s invertible repeat sequences responsible for recombinase binding are ordered incorrectly; the regions are not identical, and have ???left??? and ???right??? components. K137008 (invertible repeat-right) and K137010 (invertible repeat-left) are mislabeled and belong in the order ???K137008-region to invert-K137010??? in accordance with the wild-type fim system. Repeat sequence in this part exist in the correct (wild-type) orientation.
mutable notes: Enter design considerations
mutable provenance: Enter source
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_K137008
component: BBa_K137047
component: BBa_K137010
component: BBa_I13504
sequence annotation: BBa_K137047
sequence annotation: BBa_K137010
sequence annotation: BBa_I13504
sequence annotation: BBa_K137008
sequence: https://synbiohub.org/public/igem/BBa_K880002_sequence/1

BBa_K137008
type: Component
persistent identity: component2204543
display id: component2204543
version: 1
top level: BBa_K880002
access: public
definition: https://synbiohub.org/public/igem/BBa_K137008/1

BBa_K137047
type: Component
persistent identity: component2204545
display id: component2204545
version: 1
top level: BBa_K880002
access: public
definition: https://synbiohub.org/public/igem/BBa_K137047/1

BBa_K137010
type: Component
persistent identity: component2204548
display id: component2204548
version: 1
top level: BBa_K880002
access: public
definition: https://synbiohub.org/public/igem/BBa_K137010/1

BBa_I13504
type: Component
persistent identity: component2204558
display id: component2204558
version: 1
top level: BBa_K880002
access: public
definition: BBa_I13504

BBa_K137047
type: SequenceAnnotation
persistent identity: annotation2204545
display id: annotation2204545
version: 1
top level: BBa_K880002
location: https://synbiohub.org/public/igem/BBa_K880002/annotation2204545/range2204545/1
component: BBa_K137047

https://synbiohub.org/public/igem/BBa_K880002/annotation2204545/range2204545/1
type: Range
persistent identity: range2204545
display id: range2204545
version: 1
direction: direction/reverse
top level: BBa_K880002
start: 44
end: 293
orientation: inline

BBa_K137010
type: SequenceAnnotation
persistent identity: annotation2204548
display id: annotation2204548
version: 1
top level: BBa_K880002
location: https://synbiohub.org/public/igem/BBa_K880002/annotation2204548/range2204548/1
component: BBa_K137010

https://synbiohub.org/public/igem/BBa_K880002/annotation2204548/range2204548/1
type: Range
persistent identity: range2204548
display id: range2204548
version: 1
direction: direction/reverse
top level: BBa_K880002
start: 302
end: 336
orientation: inline

BBa_I13504
type: SequenceAnnotation
persistent identity: annotation2204558
display id: annotation2204558
version: 1
top level: BBa_K880002
location: https://synbiohub.org/public/igem/BBa_K880002/annotation2204558/range2204558/1
component: BBa_I13504

https://synbiohub.org/public/igem/BBa_K880002/annotation2204558/range2204558/1
type: Range
persistent identity: range2204558
display id: range2204558
version: 1
direction: direction/reverse
top level: BBa_K880002
start: 345
end: 1219
orientation: inline

BBa_K137008
type: SequenceAnnotation
persistent identity: annotation2204543
display id: annotation2204543
version: 1
top level: BBa_K880002
location: https://synbiohub.org/public/igem/BBa_K880002/annotation2204543/range2204543/1
component: BBa_K137008

https://synbiohub.org/public/igem/BBa_K880002/annotation2204543/range2204543/1
type: Range
persistent identity: range2204543
display id: range2204543
version: 1
direction: direction/reverse
top level: BBa_K880002
start: 1
end: 35
orientation: inline

BBa_S03858
type: ComponentDefinition
persistent identity: BBa S03858
display id: BBa_S03858
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: I718017:B0015
creator: Eimad Shotar
created: 2007-10-25T11:00:00Z
modified: 2015-05-08T01:14:31Z
discontinued: false
dominant: true
group u list: _9_
m user id: 0
owner id: 1568
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_B0010
component: BBa_I718017
component: BBa_B0012
sequence annotation: BBa_I718017
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_S03858_sequence/1

BBa_B0010
type: Component
persistent identity: component1956462
display id: component1956462
version: 1
top level: BBa_S03858
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_I718017
type: Component
persistent identity: component1956461
display id: component1956461
version: 1
top level: BBa_S03858
access: public
definition: https://synbiohub.org/public/igem/BBa_I718017/1

BBa_B0012
type: Component
persistent identity: component1956464
display id: component1956464
version: 1
top level: BBa_S03858
access: public
definition: BBa_B0012

BBa_I718017
type: SequenceAnnotation
persistent identity: annotation1956461
display id: annotation1956461
version: 1
top level: BBa_S03858
location: https://synbiohub.org/public/igem/BBa_S03858/annotation1956461/range1956461/1
component: BBa_I718017

https://synbiohub.org/public/igem/BBa_S03858/annotation1956461/range1956461/1
type: Range
persistent identity: range1956461
display id: range1956461
version: 1
direction: direction/reverse
top level: BBa_S03858
start: 1
end: 34
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1956462
display id: annotation1956462
version: 1
top level: BBa_S03858
location: https://synbiohub.org/public/igem/BBa_S03858/annotation1956462/range1956462/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_S03858/annotation1956462/range1956462/1
type: Range
persistent identity: range1956462
display id: range1956462
version: 1
direction: direction/forward
top level: BBa_S03858
start: 43
end: 122
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1956464
display id: annotation1956464
version: 1
top level: BBa_S03858
location: https://synbiohub.org/public/igem/BBa_S03858/annotation1956464/range1956464/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_S03858/annotation1956464/range1956464/1
type: Range
persistent identity: range1956464
display id: range1956464
version: 1
direction: direction/forward
top level: BBa_S03858
start: 131
end: 171
orientation: inline

BBa_K801092
type: ComponentDefinition
persistent identity: BBa K801092
display id: BBa_K801092
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 4-coumarate - coenzym A ligase (4CL) + yeast consensus sequence
creator: Ingmar Polte
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
discontinued: false
dominant: false
group u list: _1057_
m user id: 0
owner id: 13150
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: 4-coumarate - coenzym A ligase (4CL) coding region from ''Arabidopsis thaliana'' preceeded by the yeast consensus sequence for improved expression.
mutable notes: -
mutable provenance: Plasmid (template for PCR) provided by John A. Morgan, School of Chemical Engineering, Purdue University, West Lafayette, Indiana, USA http://www.ncbi.nlm.nih.gov/nucleotide/609339/ PMID: 15932991
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Consensus sequence
sequence annotation: Start codon
sequence annotation: Stop codon
sequence annotation: 4CL
sequence: https://synbiohub.org/public/igem/BBa_K801092_sequence/1

Consensus sequence
type: SequenceAnnotation
persistent identity: annotation2196267
display id: annotation2196267
version: 1
top level: BBa_K801092
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801092/annotation2196267/range2196267/1

https://synbiohub.org/public/igem/BBa_K801092/annotation2196267/range2196267/1
type: Range
persistent identity: range2196267
display id: range2196267
version: 1
direction: direction/forward
top level: BBa_K801092
start: 1
end: 6
orientation: inline

Start codon
type: SequenceAnnotation
persistent identity: annotation2201481
display id: annotation2201481
version: 1
top level: BBa_K801092
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K801092/annotation2201481/range2201481/1

https://synbiohub.org/public/igem/BBa_K801092/annotation2201481/range2201481/1
type: Range
persistent identity: range2201481
display id: range2201481
version: 1
direction: direction/forward
top level: BBa_K801092
start: 7
end: 9
orientation: inline

Stop codon
type: SequenceAnnotation
persistent identity: annotation2202114
display id: annotation2202114
version: 1
top level: BBa_K801092
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K801092/annotation2202114/range2202114/1

https://synbiohub.org/public/igem/BBa_K801092/annotation2202114/range2202114/1
type: Range
persistent identity: range2202114
display id: range2202114
version: 1
direction: direction/forward
top level: BBa_K801092
start: 1690
end: 1692
orientation: inline

4CL
type: SequenceAnnotation
persistent identity: annotation2201482
display id: annotation2201482
version: 1
top level: BBa_K801092
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K801092/annotation2201482/range2201482/1

https://synbiohub.org/public/igem/BBa_K801092/annotation2201482/range2201482/1
type: Range
persistent identity: range2201482
display id: range2201482
version: 1
direction: direction/forward
top level: BBa_K801092
start: 7
end: 1689
orientation: inline

BBa_K849003
type: ComponentDefinition
persistent identity: BBa K849003
display id: BBa_K849003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: bifunctional Cyclase from Gibberella fujikuroi
creator: Martin Schneider, Sebastian Roy
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:34Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1109_
m user id: 0
owner id: 12313
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: CPS/KS
mutable notes: no
mutable provenance: Genomic sequence of Gibberella fujikuroi.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: cds
sequence: https://synbiohub.org/public/igem/BBa_K849003_sequence/1

cds
type: SequenceAnnotation
persistent identity: annotation2202327
display id: annotation2202327
version: 1
top level: BBa_K849003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K849003/annotation2202327/range2202327/1

https://synbiohub.org/public/igem/BBa_K849003/annotation2202327/range2202327/1
type: Range
persistent identity: range2202327
display id: range2202327
version: 1
direction: direction/forward
top level: BBa_K849003
start: 1
end: 2859
orientation: inline

BBa_I13541
type: ComponentDefinition
persistent identity: BBa I13541
display id: BBa_I13541
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Intermediate in two promoter screening plasmid
creator: Josh Michener
created: 2005-08-21T11:00:00Z
modified: 2015-08-31T04:07:35Z
discontinued: false
dominant: false
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000110
role: partType/Temporary
component: BBa_B0012
component: BBa_I13453
component: BBa_B0034
component: BBa_E0040
component: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_E0040
sequence annotation: BBa_B0034
sequence annotation: BBa_B0010
sequence annotation: BBa_I13453
sequence: https://synbiohub.org/public/igem/BBa_I13541_sequence/1

BBa_B0012
type: Component
persistent identity: component1663891
display id: component1663891
version: 1
top level: BBa_I13541
access: public
definition: BBa_B0012

BBa_I13453
type: Component
persistent identity: component1663869
display id: component1663869
version: 1
top level: BBa_I13541
access: public
definition: BBa_I13453

BBa_B0034
type: Component
persistent identity: component1663874
display id: component1663874
version: 1
top level: BBa_I13541
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_E0040
type: Component
persistent identity: component1663876
display id: component1663876
version: 1
top level: BBa_I13541
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_B0010
type: Component
persistent identity: component1663881
display id: component1663881
version: 1
top level: BBa_I13541
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1663891
display id: annotation1663891
version: 1
top level: BBa_I13541
location: https://synbiohub.org/public/igem/BBa_I13541/annotation1663891/range1663891/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13541/annotation1663891/range1663891/1
type: Range
persistent identity: range1663891
display id: range1663891
version: 1
direction: direction/forward
top level: BBa_I13541
start: 973
end: 1013
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1663876
display id: annotation1663876
version: 1
top level: BBa_I13541
location: https://synbiohub.org/public/igem/BBa_I13541/annotation1663876/range1663876/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_I13541/annotation1663876/range1663876/1
type: Range
persistent identity: range1663876
display id: range1663876
version: 1
direction: direction/forward
top level: BBa_I13541
start: 157
end: 876
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1663874
display id: annotation1663874
version: 1
top level: BBa_I13541
location: https://synbiohub.org/public/igem/BBa_I13541/annotation1663874/range1663874/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13541/annotation1663874/range1663874/1
type: Range
persistent identity: range1663874
display id: range1663874
version: 1
direction: direction/forward
top level: BBa_I13541
start: 139
end: 150
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1663881
display id: annotation1663881
version: 1
top level: BBa_I13541
location: https://synbiohub.org/public/igem/BBa_I13541/annotation1663881/range1663881/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13541/annotation1663881/range1663881/1
type: Range
persistent identity: range1663881
display id: range1663881
version: 1
direction: direction/forward
top level: BBa_I13541
start: 885
end: 964
orientation: inline

BBa_I13453
type: SequenceAnnotation
persistent identity: annotation1663869
display id: annotation1663869
version: 1
top level: BBa_I13541
location: https://synbiohub.org/public/igem/BBa_I13541/annotation1663869/range1663869/1
component: BBa_I13453

https://synbiohub.org/public/igem/BBa_I13541/annotation1663869/range1663869/1
type: Range
persistent identity: range1663869
display id: range1663869
version: 1
direction: direction/forward
top level: BBa_I13541
start: 1
end: 130
orientation: inline

BBa_J202000
type: ComponentDefinition
persistent identity: BBa J202000
display id: BBa_J202000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PBAD mutant#1
creator: Jiyeon Park
created: 2012-07-18T11:00:00Z
modified: 2015-08-31T04:08:36Z
discontinued: false
dominant: false
experience: experience/None
group u list: _910_
m user id: 0
owner id: 11164
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is mutant of PBAD (BBa_I13453).
mutable notes: In order to regulate transcription from PBAD, araC binds three different sites called araO2, araI1, and araI2. In the absence of L-arabinose, araC binds both araO2 and araI1 and bends DNA preventing transcription from PBAD. In the presence of L-arabinose, however, araC has a conformational change because of L-arabinose and binds araI1 and araI2, enabling transcription from PBAD. Like the wild-type (I13453), this part lacks araO2 site which is essential for araC repressor function, araC acts as an activator in the presence of L-arabinose.
mutable provenance: This mutant was created by random mutagenesis.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Mutation
sequence annotation: araI1
sequence annotation: araC transcription start
sequence annotation: araI2
sequence: https://synbiohub.org/public/igem/BBa_J202000_sequence/1

Mutation
type: SequenceAnnotation
persistent identity: annotation2177773
display id: annotation2177773
version: 1
top level: BBa_J202000
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_J202000/annotation2177773/range2177773/1

https://synbiohub.org/public/igem/BBa_J202000/annotation2177773/range2177773/1
type: Range
persistent identity: range2177773
display id: range2177773
version: 1
direction: direction/forward
top level: BBa_J202000
start: 57
end: 57
orientation: inline

araI1
type: SequenceAnnotation
persistent identity: annotation2177770
display id: annotation2177770
version: 1
top level: BBa_J202000
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J202000/annotation2177770/range2177770/1

https://synbiohub.org/public/igem/BBa_J202000/annotation2177770/range2177770/1
type: Range
persistent identity: range2177770
display id: range2177770
version: 1
direction: direction/forward
top level: BBa_J202000
start: 40
end: 56
orientation: inline

araC transcription start
type: SequenceAnnotation
persistent identity: annotation2177772
display id: annotation2177772
version: 1
top level: BBa_J202000
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_J202000/annotation2177772/range2177772/1

https://synbiohub.org/public/igem/BBa_J202000/annotation2177772/range2177772/1
type: Range
persistent identity: range2177772
display id: range2177772
version: 1
direction: direction/forward
top level: BBa_J202000
start: 112
end: 112
orientation: inline

araI2
type: SequenceAnnotation
persistent identity: annotation2177771
display id: annotation2177771
version: 1
top level: BBa_J202000
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J202000/annotation2177771/range2177771/1

https://synbiohub.org/public/igem/BBa_J202000/annotation2177771/range2177771/1
type: Range
persistent identity: range2177771
display id: range2177771
version: 1
direction: direction/forward
top level: BBa_J202000
start: 61
end: 77
orientation: inline

BBa_J44001
type: ComponentDefinition
persistent identity: BBa J44001
display id: BBa_J44001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Reverse RBS (RBSrev) -- corresponds to BBa_B0030
creator: Todd Eckdahl
created: 2006-08-01T11:00:00Z
modified: 2015-08-31T04:08:48Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _61_71_
m user id: 0
owner id: 606
owning group id: 61
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Reverse version of RBS BBa_B0030
mutable notes: Repeats in oligos caused unusual products during cloning
mutable provenance: Cloned from synthetic oligos
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: Reverse RBS
sequence: https://synbiohub.org/public/igem/BBa_J44001_sequence/1

Reverse RBS
type: SequenceAnnotation
persistent identity: annotation1893199
display id: annotation1893199
version: 1
top level: BBa_J44001
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_J44001/annotation1893199/range1893199/1

https://synbiohub.org/public/igem/BBa_J44001/annotation1893199/range1893199/1
type: Range
persistent identity: range1893199
display id: range1893199
version: 1
direction: direction/reverse
top level: BBa_J44001
start: 1
end: 15
orientation: inline

BBa_I13027
type: ComponentDefinition
persistent identity: BBa I13027
display id: BBa_I13027
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: penI QPI intermediate
creator: jenmitch
created: 2004-07-14T11:00:00Z
modified: 2015-08-31T04:07:32Z
discontinued: false
dominant: true
experience: experience/None
group u list: _6_
m user id: 0
owner id: 159
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_B0034
component: BBa_C0074
sequence annotation: BBa_B0034
sequence annotation: BBa_C0074
sequence: https://synbiohub.org/public/igem/BBa_I13027_sequence/1

BBa_B0034
type: Component
persistent identity: component939448
display id: component939448
version: 1
top level: BBa_I13027
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_C0074
type: Component
persistent identity: component939458
display id: component939458
version: 1
top level: BBa_I13027
access: public
definition: https://synbiohub.org/public/igem/BBa_C0074/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation939448
display id: annotation939448
version: 1
top level: BBa_I13027
location: https://synbiohub.org/public/igem/BBa_I13027/annotation939448/range939448/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13027/annotation939448/range939448/1
type: Range
persistent identity: range939448
display id: range939448
version: 1
direction: direction/forward
top level: BBa_I13027
start: 1
end: 12
orientation: inline

BBa_C0074
type: SequenceAnnotation
persistent identity: annotation939458
display id: annotation939458
version: 1
top level: BBa_I13027
location: https://synbiohub.org/public/igem/BBa_I13027/annotation939458/range939458/1
component: BBa_C0074

https://synbiohub.org/public/igem/BBa_I13027/annotation939458/range939458/1
type: Range
persistent identity: range939458
display id: range939458
version: 1
direction: direction/forward
top level: BBa_I13027
start: 19
end: 441
orientation: inline

BBa_J24679
type: ComponentDefinition
persistent identity: BBa J24679
display id: BBa_J24679
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS + LacI
creator: Annie Gao
created: 2006-10-25T11:00:00Z
modified: 2015-08-31T04:08:41Z
discontinued: false
dominant: false
experience: experience/None
group u list: _58_
m user id: 0
owner id: 1017
owning group id: 58
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Adds an RBS to LacI protein coding region
mutable notes: Strength of RBS
mutable provenance: composite of other iGEM parts
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_C0012
component: BBa_B0033
sequence annotation: BBa_C0012
sequence annotation: BBa_B0033
sequence: https://synbiohub.org/public/igem/BBa_J24679_sequence/1

BBa_C0012
type: Component
persistent identity: component2243744
display id: component2243744
version: 1
top level: BBa_J24679
access: public
definition: lacI

BBa_B0033
type: Component
persistent identity: component2243741
display id: component2243741
version: 1
top level: BBa_J24679
access: public
definition: BBa_B0033

BBa_C0012
type: SequenceAnnotation
persistent identity: annotation2243744
display id: annotation2243744
version: 1
top level: BBa_J24679
location: https://synbiohub.org/public/igem/BBa_J24679/annotation2243744/range2243744/1
component: BBa_C0012

https://synbiohub.org/public/igem/BBa_J24679/annotation2243744/range2243744/1
type: Range
persistent identity: range2243744
display id: range2243744
version: 1
direction: direction/forward
top level: BBa_J24679
start: 18
end: 1145
orientation: inline

BBa_B0033
type: SequenceAnnotation
persistent identity: annotation2243741
display id: annotation2243741
version: 1
top level: BBa_J24679
location: https://synbiohub.org/public/igem/BBa_J24679/annotation2243741/range2243741/1
component: BBa_B0033

https://synbiohub.org/public/igem/BBa_J24679/annotation2243741/range2243741/1
type: Range
persistent identity: range2243741
display id: range2243741
version: 1
direction: direction/forward
top level: BBa_J24679
start: 1
end: 11
orientation: inline

BBa_K775001
type: ComponentDefinition
persistent identity: BBa K775001
display id: BBa_K775001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RI7-Olfr154 Isoamylacetate chimeric GPCR
creator: Sietske Grijseels
created: 2012-09-04T11:00:00Z
modified: 2015-05-08T01:13:15Z
discontinued: false
dominant: false
group u list: _1027_
m user id: 0
owner id: 12035
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: BBa_K775001 encodes a chimeric isoamylacetate-sensing G-protein coupled receptor which can be functionally expressed in S. cerevisiae. This chimeric receptor is composed of the ligand-binding domain of the mouse Olfr154, flanked by the N- and C- terminals of the rat OR RI7.
mutable notes: -
mutable provenance: N-terminus: Olfactory receptor-like protein I7 (olr226), Rattus norvegicus (Rat) Ligand binding domain: Olfr154 NM_013728, Mus musculus, (mouse) C-terminus: Olfactory receptor-like protein I7 (olr226), Rattus norvegicus (Rat)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K775001_sequence/1

EYFP
type: ComponentDefinition
persistent identity: BBa E0036
display id: BBa_E0036
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: enhanced yellow fluorescent protein derived from A. victoria GFP
creator: jcbraff
created: 2004-06-06T11:00:00Z
modified: 2015-08-31T04:07:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 61
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_E0036_sequence/1

BBa_K750000
type: ComponentDefinition
persistent identity: BBa K750000
display id: BBa_K750000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pBADGLT:unstable gfp expression device activated by arabinose
creator: Sifan Wang, Yizhen Yan, Jianzhao Chi, Qingshu Wu
created: 2012-09-17T11:00:00Z
modified: 2015-05-08T01:13:12Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1001_
m user id: 0
owner id: 14212
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This part contains Pbad promoter, RBS of 1.0 strength, GFP(lva) producer and double terminator. GFP(lva) will be produced after arabinose added. In the cell display part of our project, this part is used in part...part...part...
mutable notes: We use the unstable gfp for our project so the degree of fluorescence can be decreased after it get to a top point. In the cell display part, we can achieve the switch of diffrent numbers.
mutable provenance: We built this part by using the biobricks from the dna distribution plates 2012.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_K206000
component: BBa_B0034
component: BBa_B0015
component: BBa_K145015
sequence annotation: BBa_K145015
sequence annotation: BBa_K206000
sequence annotation: BBa_B0015
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K750000_sequence/1

BBa_K206000
type: Component
persistent identity: component2184509
display id: component2184509
version: 1
top level: BBa_K750000
access: public
definition: https://synbiohub.org/public/igem/BBa_K206000/1

BBa_B0034
type: Component
persistent identity: component2184511
display id: component2184511
version: 1
top level: BBa_K750000
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0015
type: Component
persistent identity: component2184524
display id: component2184524
version: 1
top level: BBa_K750000
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_K145015
type: Component
persistent identity: component2184517
display id: component2184517
version: 1
top level: BBa_K750000
access: public
definition: https://synbiohub.org/public/igem/BBa_K145015/1

BBa_K145015
type: SequenceAnnotation
persistent identity: annotation2184517
display id: annotation2184517
version: 1
top level: BBa_K750000
location: https://synbiohub.org/public/igem/BBa_K750000/annotation2184517/range2184517/1
component: BBa_K145015

https://synbiohub.org/public/igem/BBa_K750000/annotation2184517/range2184517/1
type: Range
persistent identity: range2184517
display id: range2184517
version: 1
direction: direction/reverse
top level: BBa_K750000
start: 157
end: 915
orientation: inline

BBa_K206000
type: SequenceAnnotation
persistent identity: annotation2184509
display id: annotation2184509
version: 1
top level: BBa_K750000
location: https://synbiohub.org/public/igem/BBa_K750000/annotation2184509/range2184509/1
component: BBa_K206000

https://synbiohub.org/public/igem/BBa_K750000/annotation2184509/range2184509/1
type: Range
persistent identity: range2184509
display id: range2184509
version: 1
direction: direction/reverse
top level: BBa_K750000
start: 1
end: 130
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2184524
display id: annotation2184524
version: 1
top level: BBa_K750000
location: https://synbiohub.org/public/igem/BBa_K750000/annotation2184524/range2184524/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K750000/annotation2184524/range2184524/1
type: Range
persistent identity: range2184524
display id: range2184524
version: 1
direction: direction/reverse
top level: BBa_K750000
start: 924
end: 1052
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2184511
display id: annotation2184511
version: 1
top level: BBa_K750000
location: https://synbiohub.org/public/igem/BBa_K750000/annotation2184511/range2184511/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K750000/annotation2184511/range2184511/1
type: Range
persistent identity: range2184511
display id: range2184511
version: 1
direction: direction/reverse
top level: BBa_K750000
start: 139
end: 150
orientation: inline

BBa_I732805
type: ComponentDefinition
persistent identity: BBa I732805
display id: BBa_I732805
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ARL2A0101 (RBS+, TERM-)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:59Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_B0034
component: BBa_I732105
sequence annotation: BBa_I732105
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_I732805_sequence/1

BBa_B0034
type: Component
persistent identity: component1946177
display id: component1946177
version: 1
top level: BBa_I732805
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_I732105
type: Component
persistent identity: component1946178
display id: component1946178
version: 1
top level: BBa_I732805
access: public
definition: https://synbiohub.org/public/igem/BBa_I732105/1

BBa_I732105
type: SequenceAnnotation
persistent identity: annotation1946178
display id: annotation1946178
version: 1
top level: BBa_I732805
location: https://synbiohub.org/public/igem/BBa_I732805/annotation1946178/range1946178/1
component: BBa_I732105

https://synbiohub.org/public/igem/BBa_I732805/annotation1946178/range1946178/1
type: Range
persistent identity: range1946178
display id: range1946178
version: 1
direction: direction/reverse
top level: BBa_I732805
start: 19
end: 1104
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1946177
display id: annotation1946177
version: 1
top level: BBa_I732805
location: https://synbiohub.org/public/igem/BBa_I732805/annotation1946177/range1946177/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I732805/annotation1946177/range1946177/1
type: Range
persistent identity: range1946177
display id: range1946177
version: 1
direction: direction/reverse
top level: BBa_I732805
start: 1
end: 12
orientation: inline

BBa_E5503
type: ComponentDefinition
persistent identity: BBa E5503
display id: BBa_E5503
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: R0040.B0030.E0040
creator: jcbraff
created: 2005-05-16T11:00:00Z
modified: 2015-08-31T04:07:27Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 61
owning group id: 1
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: R0040.B0030.E0040 (R0040.E5500)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0030
component: BBa_R0040
component: BBa_E0040
sequence annotation: BBa_E0040
sequence annotation: BBa_B0030
sequence annotation: BBa_R0040
sequence: https://synbiohub.org/public/igem/BBa_E5503_sequence/1

BBa_B0030
type: Component
persistent identity: component1489147
display id: component1489147
version: 1
top level: BBa_E5503
access: public
definition: BBa_B0030

BBa_R0040
type: Component
persistent identity: component1489136
display id: component1489136
version: 1
top level: BBa_E5503
access: public
definition: p(tetR)

BBa_E0040
type: Component
persistent identity: component1489150
display id: component1489150
version: 1
top level: BBa_E5503
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1489150
display id: annotation1489150
version: 1
top level: BBa_E5503
location: https://synbiohub.org/public/igem/BBa_E5503/annotation1489150/range1489150/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_E5503/annotation1489150/range1489150/1
type: Range
persistent identity: range1489150
display id: range1489150
version: 1
direction: direction/forward
top level: BBa_E5503
start: 84
end: 803
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1489147
display id: annotation1489147
version: 1
top level: BBa_E5503
location: https://synbiohub.org/public/igem/BBa_E5503/annotation1489147/range1489147/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_E5503/annotation1489147/range1489147/1
type: Range
persistent identity: range1489147
display id: range1489147
version: 1
direction: direction/forward
top level: BBa_E5503
start: 63
end: 77
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1489136
display id: annotation1489136
version: 1
top level: BBa_E5503
location: https://synbiohub.org/public/igem/BBa_E5503/annotation1489136/range1489136/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_E5503/annotation1489136/range1489136/1
type: Range
persistent identity: range1489136
display id: range1489136
version: 1
direction: direction/forward
top level: BBa_E5503
start: 1
end: 54
orientation: inline

RBS-LacY
type: ComponentDefinition
persistent identity: BBa I763015
display id: BBa_I763015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS-LacY
creator: Alice Pasini
created: 2007-08-19T11:00:00Z
modified: 2015-08-31T04:08:08Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _111_
m user id: 0
owner id: 2088
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: PLac activation induces the expression of the Lactose permase (Lac Y) gene, LacI repressor protein with GFP (+LVA tag) as a reporter.
mutable notes: ---
mutable provenance: Igem 2007 plates
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_J22101
component: BBa_B0034
sequence annotation: BBa_J22101
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_I763015_sequence/1

BBa_J22101
type: Component
persistent identity: component1943313
display id: component1943313
version: 1
top level: RBS-LacY
access: public
definition: https://synbiohub.org/public/igem/BBa_J22101/1

BBa_B0034
type: Component
persistent identity: component1943312
display id: component1943312
version: 1
top level: RBS-LacY
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_J22101
type: SequenceAnnotation
persistent identity: annotation1943313
display id: annotation1943313
version: 1
top level: RBS-LacY
location: https://synbiohub.org/public/igem/BBa_I763015/annotation1943313/range1943313/1
component: BBa_J22101

https://synbiohub.org/public/igem/BBa_I763015/annotation1943313/range1943313/1
type: Range
persistent identity: range1943313
display id: range1943313
version: 1
direction: direction/reverse
top level: RBS-LacY
start: 19
end: 1306
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1943312
display id: annotation1943312
version: 1
top level: RBS-LacY
location: https://synbiohub.org/public/igem/BBa_I763015/annotation1943312/range1943312/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I763015/annotation1943312/range1943312/1
type: Range
persistent identity: range1943312
display id: range1943312
version: 1
direction: direction/reverse
top level: RBS-LacY
start: 1
end: 12
orientation: inline

BBa_K817000
type: ComponentDefinition
persistent identity: BBa K817000
display id: BBa_K817000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SP1-GLP1
creator: Yen-Der Li
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:28Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1075_
m user id: 0
owner id: 13572
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This is the GLP-1 coding part along with the signal peptide sequence.
mutable notes: SP1-GLP1
mutable provenance: SP1-GLP1
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: GLP-1
sequence annotation: SP1
sequence: https://synbiohub.org/public/igem/BBa_K817000_sequence/1

GLP-1
type: SequenceAnnotation
persistent identity: annotation2201480
display id: annotation2201480
version: 1
top level: BBa_K817000
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K817000/annotation2201480/range2201480/1

https://synbiohub.org/public/igem/BBa_K817000/annotation2201480/range2201480/1
type: Range
persistent identity: range2201480
display id: range2201480
version: 1
direction: direction/forward
top level: BBa_K817000
start: 65
end: 162
orientation: inline

SP1
type: SequenceAnnotation
persistent identity: annotation2201479
display id: annotation2201479
version: 1
top level: BBa_K817000
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K817000/annotation2201479/range2201479/1

https://synbiohub.org/public/igem/BBa_K817000/annotation2201479/range2201479/1
type: Range
persistent identity: range2201479
display id: range2201479
version: 1
direction: direction/forward
top level: BBa_K817000
start: 1
end: 64
orientation: inline

BBa_R2109
type: ComponentDefinition
persistent identity: BBa R2109
display id: BBa_R2109
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter with operator site for C2003
creator: Reshma Shetty
created: 2006-10-20T11:00:00Z
modified: 2015-05-08T01:14:16Z
discontinued: false
dominant: false
group u list: _41_
m user id: 0
owner id: 126
owning group id: 70
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This promoter is designed to bind to C2003.
mutable notes: Placement of the operator site was determined to optimize steric interference between C2003 and RNA polymerase.
mutable provenance: This promoter was rationally designed and synthesized.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: operator site for C2003
sequence annotation: transcription start site
sequence annotation: -35 site
sequence annotation: -10 site
sequence: https://synbiohub.org/public/igem/BBa_R2109_sequence/1

operator site for C2003
type: SequenceAnnotation
persistent identity: annotation1903915
display id: annotation1903915
version: 1
top level: BBa_R2109
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_R2109/annotation1903915/range1903915/1

https://synbiohub.org/public/igem/BBa_R2109/annotation1903915/range1903915/1
type: Range
persistent identity: range1903915
display id: range1903915
version: 1
direction: direction/forward
top level: BBa_R2109
start: 22
end: 33
orientation: inline

transcription start site
type: SequenceAnnotation
persistent identity: annotation1903914
display id: annotation1903914
version: 1
top level: BBa_R2109
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_R2109/annotation1903914/range1903914/1

https://synbiohub.org/public/igem/BBa_R2109/annotation1903914/range1903914/1
type: Range
persistent identity: range1903914
display id: range1903914
version: 1
direction: direction/forward
top level: BBa_R2109
start: 53
end: 53
orientation: inline

-35 site
type: SequenceAnnotation
persistent identity: annotation1903912
display id: annotation1903912
version: 1
top level: BBa_R2109
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R2109/annotation1903912/range1903912/1

https://synbiohub.org/public/igem/BBa_R2109/annotation1903912/range1903912/1
type: Range
persistent identity: range1903912
display id: range1903912
version: 1
direction: direction/forward
top level: BBa_R2109
start: 17
end: 22
orientation: inline

-10 site
type: SequenceAnnotation
persistent identity: annotation1903913
display id: annotation1903913
version: 1
top level: BBa_R2109
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R2109/annotation1903913/range1903913/1

https://synbiohub.org/public/igem/BBa_R2109/annotation1903913/range1903913/1
type: Range
persistent identity: range1903913
display id: range1903913
version: 1
direction: direction/forward
top level: BBa_R2109
start: 40
end: 45
orientation: inline

BBa_K863000
type: ComponentDefinition
persistent identity: BBa K863000
display id: BBa_K863000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: bpul (laccase from Bacillus pumilus) with T7 promoter, RBS and HIS tag
creator: Isabel Huber
created: 2012-09-17T11:00:00Z
modified: 2015-05-08T01:13:36Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1123_
m user id: 0
owner id: 12844
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: bpul (Laccase from Bacillus pumilus) with T7, RBS and HIS tag
mutable notes: test
mutable provenance: Bacillus pumilus DSM 27 (ATCC7061)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: RBS
sequence annotation: ATG
sequence annotation: TAA
sequence annotation: 6 x HIS
sequence annotation: bpul
sequence annotation: A (Original) T (Mutation)
sequence annotation: T7- Promotor BBa_I719005
sequence: https://synbiohub.org/public/igem/BBa_K863000_sequence/1

RBS
type: SequenceAnnotation
persistent identity: annotation2184909
display id: annotation2184909
version: 1
top level: BBa_K863000
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K863000/annotation2184909/range2184909/1

https://synbiohub.org/public/igem/BBa_K863000/annotation2184909/range2184909/1
type: Range
persistent identity: range2184909
display id: range2184909
version: 1
direction: direction/forward
top level: BBa_K863000
start: 25
end: 30
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2184910
display id: annotation2184910
version: 1
top level: BBa_K863000
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K863000/annotation2184910/range2184910/1

https://synbiohub.org/public/igem/BBa_K863000/annotation2184910/range2184910/1
type: Range
persistent identity: range2184910
display id: range2184910
version: 1
direction: direction/forward
top level: BBa_K863000
start: 34
end: 36
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2184914
display id: annotation2184914
version: 1
top level: BBa_K863000
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K863000/annotation2184914/range2184914/1

https://synbiohub.org/public/igem/BBa_K863000/annotation2184914/range2184914/1
type: Range
persistent identity: range2184914
display id: range2184914
version: 1
direction: direction/forward
top level: BBa_K863000
start: 1582
end: 1584
orientation: inline

6 x HIS
type: SequenceAnnotation
persistent identity: annotation2184913
display id: annotation2184913
version: 1
top level: BBa_K863000
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K863000/annotation2184913/range2184913/1

https://synbiohub.org/public/igem/BBa_K863000/annotation2184913/range2184913/1
type: Range
persistent identity: range2184913
display id: range2184913
version: 1
direction: direction/forward
top level: BBa_K863000
start: 1557
end: 1581
orientation: inline

bpul
type: SequenceAnnotation
persistent identity: annotation2184911
display id: annotation2184911
version: 1
top level: BBa_K863000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K863000/annotation2184911/range2184911/1

https://synbiohub.org/public/igem/BBa_K863000/annotation2184911/range2184911/1
type: Range
persistent identity: range2184911
display id: range2184911
version: 1
direction: direction/forward
top level: BBa_K863000
start: 34
end: 1556
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2184915
display id: annotation2184915
version: 1
top level: BBa_K863000
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K863000/annotation2184915/range2184915/1

https://synbiohub.org/public/igem/BBa_K863000/annotation2184915/range2184915/1
type: Range
persistent identity: range2184915
display id: range2184915
version: 1
direction: direction/forward
top level: BBa_K863000
start: 1585
end: 1587
orientation: inline

A (Original) T (Mutation)
type: SequenceAnnotation
persistent identity: annotation2184912
display id: annotation2184912
version: 1
top level: BBa_K863000
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K863000/annotation2184912/range2184912/1

https://synbiohub.org/public/igem/BBa_K863000/annotation2184912/range2184912/1
type: Range
persistent identity: range2184912
display id: range2184912
version: 1
direction: direction/forward
top level: BBa_K863000
start: 834
end: 834
orientation: inline

T7- Promotor BBa_I719005
type: SequenceAnnotation
persistent identity: annotation2184908
display id: annotation2184908
version: 1
top level: BBa_K863000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K863000/annotation2184908/range2184908/1

https://synbiohub.org/public/igem/BBa_K863000/annotation2184908/range2184908/1
type: Range
persistent identity: range2184908
display id: range2184908
version: 1
direction: direction/forward
top level: BBa_K863000
start: 1
end: 23
orientation: inline

BBa_J23119
type: ComponentDefinition
persistent identity: BBa J23119
display id: BBa_J23119
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter family member
creator: John Anderson
created: 2006-08-23T11:00:00Z
modified: 2015-08-31T04:08:40Z
discontinued: false
dominant: true
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Later
mutable notes: N/A
mutable provenance: Overlap extension of synthetic oligonucleotides
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23119_sequence/1

BBa_K936013
type: ComponentDefinition
persistent identity: BBa K936013
display id: BBa_K936013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pelB-LC-Cutinase fusion protein
creator: Mattan Hamou
created: 2012-07-23T11:00:00Z
modified: 2015-05-08T01:13:47Z
discontinued: false
dominant: false
group u list: _1201_
m user id: 0
owner id: 13899
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: pelB leader sequence attached to enzyme LC-Cutinase protein coding sequence in order to allow the cell to bring the enzyme to the periplasm for easier secretion.
mutable notes: Adding sequence of pelB in front of sequence for Cuntinase
mutable provenance: http://aem.asm.org/content/78/5/1556.long Constructed from pelB leader sequence and protein coding sequence for LC-Cutinase
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: BBa_J32015
sequence annotation: BBa_K936000
sequence annotation: PelB
sequence: https://synbiohub.org/public/igem/BBa_K936013_sequence/1

BBa_J32015
type: SequenceAnnotation
persistent identity: annotation2178084
display id: annotation2178084
version: 1
top level: BBa_K936013
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K936013/annotation2178084/range2178084/1

https://synbiohub.org/public/igem/BBa_K936013/annotation2178084/range2178084/1
type: Range
persistent identity: range2178084
display id: range2178084
version: 1
direction: direction/reverse
top level: BBa_K936013
start: 1
end: 66
orientation: inline

BBa_K936000
type: SequenceAnnotation
persistent identity: annotation2178085
display id: annotation2178085
version: 1
top level: BBa_K936013
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K936013/annotation2178085/range2178085/1

https://synbiohub.org/public/igem/BBa_K936013/annotation2178085/range2178085/1
type: Range
persistent identity: range2178085
display id: range2178085
version: 1
direction: direction/reverse
top level: BBa_K936013
start: 67
end: 951
orientation: inline

PelB
type: SequenceAnnotation
persistent identity: annotation2178083
display id: annotation2178083
version: 1
top level: BBa_K936013
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K936013/annotation2178083/range2178083/1

https://synbiohub.org/public/igem/BBa_K936013/annotation2178083/range2178083/1
type: Range
persistent identity: range2178083
display id: range2178083
version: 1
direction: direction/forward
top level: BBa_K936013
start: 1
end: 66
orientation: inline

BBa_I732095
type: ComponentDefinition
persistent identity: BBa I732095
display id: BBa_I732095
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Activity Reporter (LacZ-alpha and RFP)
creator: Zhan Jian
created: 2007-08-31T11:00:00Z
modified: 2015-08-31T04:07:57Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_I13507
component: BBa_I732074
sequence annotation: BBa_I13507
sequence annotation: BBa_I732074
sequence: https://synbiohub.org/public/igem/BBa_I732095_sequence/1

BBa_I13507
type: Component
persistent identity: component2265804
display id: component2265804
version: 1
top level: BBa_I732095
access: public
definition: https://synbiohub.org/public/igem/BBa_I13507/1

BBa_I732074
type: Component
persistent identity: component2265792
display id: component2265792
version: 1
top level: BBa_I732095
access: public
definition: BBa_I732074

BBa_I13507
type: SequenceAnnotation
persistent identity: annotation2265804
display id: annotation2265804
version: 1
top level: BBa_I732095
location: https://synbiohub.org/public/igem/BBa_I732095/annotation2265804/range2265804/1
component: BBa_I13507

https://synbiohub.org/public/igem/BBa_I732095/annotation2265804/range2265804/1
type: Range
persistent identity: range2265804
display id: range2265804
version: 1
direction: direction/reverse
top level: BBa_I732095
start: 288
end: 1148
orientation: inline

BBa_I732074
type: SequenceAnnotation
persistent identity: annotation2265792
display id: annotation2265792
version: 1
top level: BBa_I732095
location: https://synbiohub.org/public/igem/BBa_I732095/annotation2265792/range2265792/1
component: BBa_I732074

https://synbiohub.org/public/igem/BBa_I732095/annotation2265792/range2265792/1
type: Range
persistent identity: range2265792
display id: range2265792
version: 1
direction: direction/reverse
top level: BBa_I732095
start: 1
end: 279
orientation: inline

BBa_K863011
type: ComponentDefinition
persistent identity: BBa K863011
display id: BBa_K863011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: tthl laccase from Thermus thermophilus
creator: Isabel Huber
created: 2012-09-17T11:00:00Z
modified: 2015-05-08T01:13:36Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1123_
m user id: 0
owner id: 12844
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: tthl (Laccase from Thermus thermophilus) coding sequence
mutable notes: test
mutable provenance: Thermus thermophilus HB27
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: TAG
sequence annotation: ATG
sequence annotation: tthl
sequence: https://synbiohub.org/public/igem/BBa_K863011_sequence/1

TAG
type: SequenceAnnotation
persistent identity: annotation2184889
display id: annotation2184889
version: 1
top level: BBa_K863011
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K863011/annotation2184889/range2184889/1

https://synbiohub.org/public/igem/BBa_K863011/annotation2184889/range2184889/1
type: Range
persistent identity: range2184889
display id: range2184889
version: 1
direction: direction/forward
top level: BBa_K863011
start: 1387
end: 1389
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2184806
display id: annotation2184806
version: 1
top level: BBa_K863011
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K863011/annotation2184806/range2184806/1

https://synbiohub.org/public/igem/BBa_K863011/annotation2184806/range2184806/1
type: Range
persistent identity: range2184806
display id: range2184806
version: 1
direction: direction/forward
top level: BBa_K863011
start: 1
end: 3
orientation: inline

tthl
type: SequenceAnnotation
persistent identity: annotation2184888
display id: annotation2184888
version: 1
top level: BBa_K863011
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K863011/annotation2184888/range2184888/1

https://synbiohub.org/public/igem/BBa_K863011/annotation2184888/range2184888/1
type: Range
persistent identity: range2184888
display id: range2184888
version: 1
direction: direction/forward
top level: BBa_K863011
start: 1
end: 1389
orientation: inline

BBa_K759012
type: ComponentDefinition
persistent identity: BBa K759012
display id: BBa_K759012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: eCFP + E. coli aggregation (Ag43) device induced by pBAD promoter.
creator: Kenta Mine
created: 2012-09-19T11:00:00Z
modified: 2015-05-08T01:13:13Z
discontinued: false
dominant: false
group u list: _1010_
m user id: 0
owner id: 10980
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: In the presence of L-arabinose, pBAD promoter is induced and then wildtype Antigen 43 (Ag43) protein and eCFP (engineered cyan fluorescent protein)is produced. The wildtype Ag43 have 6 PstI sites. eCFP locates in the upstream of Ag43 for estimating the relationship between expressed protein amount and aggregation efficiency.
mutable notes: 6 PstI sites exists in Ag43 coding site so we assembled this composite part without using PstI.
mutable provenance: Ag43(BBA_K346007) was derived from E. coli K-12 strain by Peking 2010 iGEM.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K346007
component: BBa_I0500
component: BBa_B0034
component: BBa_B0015
component: BBa_E0020
sequence annotation: BBa_E0020
sequence annotation: BBa_B0034
sequence annotation: BBa_B0015
sequence annotation: BBa_K346007
sequence annotation: BBa_I0500
sequence: https://synbiohub.org/public/igem/BBa_K759012_sequence/1

BBa_K346007
type: Component
persistent identity: component2188725
display id: component2188725
version: 1
top level: BBa_K759012
access: public
definition: https://synbiohub.org/public/igem/BBa_K346007/1

BBa_I0500
type: Component
persistent identity: component2188718
display id: component2188718
version: 1
top level: BBa_K759012
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_B0034
type: Component
persistent identity: component2188723
display id: component2188723
version: 1
top level: BBa_K759012
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0015
type: Component
persistent identity: component2188732
display id: component2188732
version: 1
top level: BBa_K759012
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_E0020
type: Component
persistent identity: component2188721
display id: component2188721
version: 1
top level: BBa_K759012
access: public
definition: ecfp

BBa_B0034
type: Component
persistent identity: component2188720
display id: component2188720
version: 1
top level: BBa_K759012
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_E0020
type: SequenceAnnotation
persistent identity: annotation2188721
display id: annotation2188721
version: 1
top level: BBa_K759012
location: https://synbiohub.org/public/igem/BBa_K759012/annotation2188721/range2188721/1
component: BBa_E0020

https://synbiohub.org/public/igem/BBa_K759012/annotation2188721/range2188721/1
type: Range
persistent identity: range2188721
display id: range2188721
version: 1
direction: direction/reverse
top level: BBa_K759012
start: 1237
end: 1959
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2188720
display id: annotation2188720
version: 1
top level: BBa_K759012
location: https://synbiohub.org/public/igem/BBa_K759012/annotation2188720/range2188720/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K759012/annotation2188720/range2188720/1
type: Range
persistent identity: range2188720
display id: range2188720
version: 1
direction: direction/reverse
top level: BBa_K759012
start: 1219
end: 1230
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2188732
display id: annotation2188732
version: 1
top level: BBa_K759012
location: https://synbiohub.org/public/igem/BBa_K759012/annotation2188732/range2188732/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K759012/annotation2188732/range2188732/1
type: Range
persistent identity: range2188732
display id: range2188732
version: 1
direction: direction/reverse
top level: BBa_K759012
start: 5114
end: 5242
orientation: inline

BBa_K346007
type: SequenceAnnotation
persistent identity: annotation2188725
display id: annotation2188725
version: 1
top level: BBa_K759012
location: https://synbiohub.org/public/igem/BBa_K759012/annotation2188725/range2188725/1
component: BBa_K346007

https://synbiohub.org/public/igem/BBa_K759012/annotation2188725/range2188725/1
type: Range
persistent identity: range2188725
display id: range2188725
version: 1
direction: direction/reverse
top level: BBa_K759012
start: 1986
end: 5105
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2188723
display id: annotation2188723
version: 1
top level: BBa_K759012
location: https://synbiohub.org/public/igem/BBa_K759012/annotation2188723/range2188723/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K759012/annotation2188723/range2188723/1
type: Range
persistent identity: range2188723
display id: range2188723
version: 1
direction: direction/reverse
top level: BBa_K759012
start: 1968
end: 1979
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2188718
display id: annotation2188718
version: 1
top level: BBa_K759012
location: https://synbiohub.org/public/igem/BBa_K759012/annotation2188718/range2188718/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_K759012/annotation2188718/range2188718/1
type: Range
persistent identity: range2188718
display id: range2188718
version: 1
direction: direction/reverse
top level: BBa_K759012
start: 1
end: 1210
orientation: inline

BBa_I6060
type: ComponentDefinition
persistent identity: BBa I6060
display id: BBa_I6060
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter O_H Test R0010.E0432
creator: Caitlin Conboy and Jennifer Braff
created: 2004-03-16T12:00:00Z
modified: 2015-08-31T04:07:43Z
discontinued: false
dominant: true
experience: experience/Issues
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_B0012
component: BBa_R0010
component: BBa_E0032
component: BBa_B0010
component: BBa_B0034
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_R0010
sequence annotation: BBa_E0032
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_I6060_sequence/1

BBa_B0012
type: Component
persistent identity: component948926
display id: component948926
version: 1
top level: BBa_I6060
access: public
definition: BBa_B0012

BBa_R0010
type: Component
persistent identity: component948886
display id: component948886
version: 1
top level: BBa_I6060
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_E0032
type: Component
persistent identity: component948908
display id: component948908
version: 1
top level: BBa_I6060
access: public
definition: YFP

BBa_B0010
type: Component
persistent identity: component948916
display id: component948916
version: 1
top level: BBa_I6060
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component948896
display id: component948896
version: 1
top level: BBa_I6060
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation948916
display id: annotation948916
version: 1
top level: BBa_I6060
location: https://synbiohub.org/public/igem/BBa_I6060/annotation948916/range948916/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I6060/annotation948916/range948916/1
type: Range
persistent identity: range948916
display id: range948916
version: 1
direction: direction/forward
top level: BBa_I6060
start: 997
end: 1076
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation948926
display id: annotation948926
version: 1
top level: BBa_I6060
location: https://synbiohub.org/public/igem/BBa_I6060/annotation948926/range948926/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I6060/annotation948926/range948926/1
type: Range
persistent identity: range948926
display id: range948926
version: 1
direction: direction/forward
top level: BBa_I6060
start: 1085
end: 1125
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation948886
display id: annotation948886
version: 1
top level: BBa_I6060
location: https://synbiohub.org/public/igem/BBa_I6060/annotation948886/range948886/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_I6060/annotation948886/range948886/1
type: Range
persistent identity: range948886
display id: range948886
version: 1
direction: direction/forward
top level: BBa_I6060
start: 1
end: 200
orientation: inline

BBa_E0032
type: SequenceAnnotation
persistent identity: annotation948908
display id: annotation948908
version: 1
top level: BBa_I6060
location: https://synbiohub.org/public/igem/BBa_I6060/annotation948908/range948908/1
component: BBa_E0032

https://synbiohub.org/public/igem/BBa_I6060/annotation948908/range948908/1
type: Range
persistent identity: range948908
display id: range948908
version: 1
direction: direction/forward
top level: BBa_I6060
start: 227
end: 988
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation948896
display id: annotation948896
version: 1
top level: BBa_I6060
location: https://synbiohub.org/public/igem/BBa_I6060/annotation948896/range948896/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I6060/annotation948896/range948896/1
type: Range
persistent identity: range948896
display id: range948896
version: 1
direction: direction/forward
top level: BBa_I6060
start: 209
end: 220
orientation: inline

BBa_E0422
type: ComponentDefinition
persistent identity: BBa E0422
display id: BBa_E0422
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ECFP (RBS+ LVA+ TERM) (B0034.E0022.B0015)
creator: Caitlin Conboy and Jennifer Braff
created: 2003-12-04T12:00:00Z
modified: 2015-08-31T04:07:26Z
discontinued: false
dominant: true
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0034
component: BBa_B0010
component: BBa_E0022
component: BBa_B0012
sequence annotation: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_E0022
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_E0422_sequence/1

BBa_B0034
type: Component
persistent identity: component942803
display id: component942803
version: 1
top level: BBa_E0422
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component942823
display id: component942823
version: 1
top level: BBa_E0422
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_E0022
type: Component
persistent identity: component942815
display id: component942815
version: 1
top level: BBa_E0422
access: public
definition: ECFP

BBa_B0012
type: Component
persistent identity: component942833
display id: component942833
version: 1
top level: BBa_E0422
access: public
definition: BBa_B0012

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation942833
display id: annotation942833
version: 1
top level: BBa_E0422
location: https://synbiohub.org/public/igem/BBa_E0422/annotation942833/range942833/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_E0422/annotation942833/range942833/1
type: Range
persistent identity: range942833
display id: range942833
version: 1
direction: direction/forward
top level: BBa_E0422
start: 877
end: 917
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation942803
display id: annotation942803
version: 1
top level: BBa_E0422
location: https://synbiohub.org/public/igem/BBa_E0422/annotation942803/range942803/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_E0422/annotation942803/range942803/1
type: Range
persistent identity: range942803
display id: range942803
version: 1
direction: direction/forward
top level: BBa_E0422
start: 1
end: 12
orientation: inline

BBa_E0022
type: SequenceAnnotation
persistent identity: annotation942815
display id: annotation942815
version: 1
top level: BBa_E0422
location: https://synbiohub.org/public/igem/BBa_E0422/annotation942815/range942815/1
component: BBa_E0022

https://synbiohub.org/public/igem/BBa_E0422/annotation942815/range942815/1
type: Range
persistent identity: range942815
display id: range942815
version: 1
direction: direction/forward
top level: BBa_E0422
start: 19
end: 780
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation942823
display id: annotation942823
version: 1
top level: BBa_E0422
location: https://synbiohub.org/public/igem/BBa_E0422/annotation942823/range942823/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_E0422/annotation942823/range942823/1
type: Range
persistent identity: range942823
display id: range942823
version: 1
direction: direction/forward
top level: BBa_E0422
start: 789
end: 868
orientation: inline

tctA_503aa
type: ComponentDefinition
persistent identity: BBa K808002
display id: BBa_K808002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: tctA_503: large subunit A1 of the tripartite tricarboxylate transporter family
creator: Valentina Herbring, Sebastian Palluk, Andreas Schmidt
created: 2012-09-04T11:00:00Z
modified: 2015-05-08T01:13:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1065_
m user id: 0
owner id: 13606
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: tctA_503: large subunit A1 of the tripartite tricarboxylate transporter family
mutable notes: .
mutable provenance: Comamonas tesosteroni KF1
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K808002_sequence/1

BBa_I732097
type: ComponentDefinition
persistent identity: BBa I732097
display id: BBa_I732097
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Activity Reporter (RFP)
creator: Zhan Jian
created: 2007-10-16T11:00:00Z
modified: 2015-08-31T04:07:57Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Promoter can be inserted into the adapter containing BglII and BamHI sites. The activity of inserted promoter can be quantificationally measured using ONPG assay or fluorescent assay.
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0015
component: BBa_I732007
component: BBa_B0034
component: BBa_E1010
sequence annotation: BBa_E1010
sequence annotation: BBa_B0015
sequence annotation: BBa_B0034
sequence annotation: BBa_I732007
sequence: https://synbiohub.org/public/igem/BBa_I732097_sequence/1

BBa_B0015
type: Component
persistent identity: component2253310
display id: component2253310
version: 1
top level: BBa_I732097
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_I732007
type: Component
persistent identity: component2253298
display id: component2253298
version: 1
top level: BBa_I732097
access: public
definition: Adapter

BBa_B0034
type: Component
persistent identity: component2253300
display id: component2253300
version: 1
top level: BBa_I732097
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_E1010
type: Component
persistent identity: component2253303
display id: component2253303
version: 1
top level: BBa_I732097
access: public
definition: https://synbiohub.org/public/igem/BBa_E1010/1

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2253303
display id: annotation2253303
version: 1
top level: BBa_I732097
location: https://synbiohub.org/public/igem/BBa_I732097/annotation2253303/range2253303/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_I732097/annotation2253303/range2253303/1
type: Range
persistent identity: range2253303
display id: range2253303
version: 1
direction: direction/reverse
top level: BBa_I732097
start: 46
end: 751
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2253310
display id: annotation2253310
version: 1
top level: BBa_I732097
location: https://synbiohub.org/public/igem/BBa_I732097/annotation2253310/range2253310/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_I732097/annotation2253310/range2253310/1
type: Range
persistent identity: range2253310
display id: range2253310
version: 1
direction: direction/reverse
top level: BBa_I732097
start: 760
end: 888
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2253300
display id: annotation2253300
version: 1
top level: BBa_I732097
location: https://synbiohub.org/public/igem/BBa_I732097/annotation2253300/range2253300/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I732097/annotation2253300/range2253300/1
type: Range
persistent identity: range2253300
display id: range2253300
version: 1
direction: direction/reverse
top level: BBa_I732097
start: 28
end: 39
orientation: inline

BBa_I732007
type: SequenceAnnotation
persistent identity: annotation2253298
display id: annotation2253298
version: 1
top level: BBa_I732097
location: https://synbiohub.org/public/igem/BBa_I732097/annotation2253298/range2253298/1
component: BBa_I732007

https://synbiohub.org/public/igem/BBa_I732097/annotation2253298/range2253298/1
type: Range
persistent identity: range2253298
display id: range2253298
version: 1
direction: direction/reverse
top level: BBa_I732097
start: 1
end: 19
orientation: inline

BBa_I6046
type: ComponentDefinition
persistent identity: BBa I6046
display id: BBa_I6046
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter O_H Test R0065.E0430
creator: Caitlin Conboy and Jennifer Braff
created: 2004-03-16T12:00:00Z
modified: 2015-08-31T04:07:43Z
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_B0010
component: BBa_B0034
component: BBa_E0030
component: BBa_R0065
component: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_R0065
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_E0030
sequence: https://synbiohub.org/public/igem/BBa_I6046_sequence/1

BBa_B0010
type: Component
persistent identity: component948627
display id: component948627
version: 1
top level: BBa_I6046
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component948620
display id: component948620
version: 1
top level: BBa_I6046
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_E0030
type: Component
persistent identity: component948622
display id: component948622
version: 1
top level: BBa_I6046
access: public
definition: https://synbiohub.org/public/igem/BBa_E0030/1

BBa_R0065
type: Component
persistent identity: component948608
display id: component948608
version: 1
top level: BBa_I6046
access: public
definition: cI+luxR

BBa_B0012
type: Component
persistent identity: component948637
display id: component948637
version: 1
top level: BBa_I6046
access: public
definition: BBa_B0012

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation948620
display id: annotation948620
version: 1
top level: BBa_I6046
location: https://synbiohub.org/public/igem/BBa_I6046/annotation948620/range948620/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I6046/annotation948620/range948620/1
type: Range
persistent identity: range948620
display id: range948620
version: 1
direction: direction/forward
top level: BBa_I6046
start: 106
end: 117
orientation: inline

BBa_R0065
type: SequenceAnnotation
persistent identity: annotation948608
display id: annotation948608
version: 1
top level: BBa_I6046
location: https://synbiohub.org/public/igem/BBa_I6046/annotation948608/range948608/1
component: BBa_R0065

https://synbiohub.org/public/igem/BBa_I6046/annotation948608/range948608/1
type: Range
persistent identity: range948608
display id: range948608
version: 1
direction: direction/forward
top level: BBa_I6046
start: 1
end: 97
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation948627
display id: annotation948627
version: 1
top level: BBa_I6046
location: https://synbiohub.org/public/igem/BBa_I6046/annotation948627/range948627/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I6046/annotation948627/range948627/1
type: Range
persistent identity: range948627
display id: range948627
version: 1
direction: direction/forward
top level: BBa_I6046
start: 855
end: 934
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation948637
display id: annotation948637
version: 1
top level: BBa_I6046
location: https://synbiohub.org/public/igem/BBa_I6046/annotation948637/range948637/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I6046/annotation948637/range948637/1
type: Range
persistent identity: range948637
display id: range948637
version: 1
direction: direction/forward
top level: BBa_I6046
start: 943
end: 983
orientation: inline

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation948622
display id: annotation948622
version: 1
top level: BBa_I6046
location: https://synbiohub.org/public/igem/BBa_I6046/annotation948622/range948622/1
component: BBa_E0030

https://synbiohub.org/public/igem/BBa_I6046/annotation948622/range948622/1
type: Range
persistent identity: range948622
display id: range948622
version: 1
direction: direction/forward
top level: BBa_I6046
start: 124
end: 846
orientation: inline

BBa_K916000
type: ComponentDefinition
persistent identity: BBa K916000
display id: BBa_K916000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TraR Coding Sequence
creator: Joseph Elsherbini
created: 2012-09-25T11:00:00Z
modified: 2015-05-08T01:13:46Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1181_
m user id: 0
owner id: 4259
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: TraR Coding Sequence from Agrobacterium tumefaciens
mutable notes: -
mutable provenance: plasmid containing TraR
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: cds
sequence annotation: stop
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_K916000_sequence/1

cds
type: SequenceAnnotation
persistent identity: annotation2202115
display id: annotation2202115
version: 1
top level: BBa_K916000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K916000/annotation2202115/range2202115/1

https://synbiohub.org/public/igem/BBa_K916000/annotation2202115/range2202115/1
type: Range
persistent identity: range2202115
display id: range2202115
version: 1
direction: direction/forward
top level: BBa_K916000
start: 1
end: 705
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2202116
display id: annotation2202116
version: 1
top level: BBa_K916000
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K916000/annotation2202116/range2202116/1

https://synbiohub.org/public/igem/BBa_K916000/annotation2202116/range2202116/1
type: Range
persistent identity: range2202116
display id: range2202116
version: 1
direction: direction/forward
top level: BBa_K916000
start: 703
end: 705
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2202117
display id: annotation2202117
version: 1
top level: BBa_K916000
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K916000/annotation2202117/range2202117/1

https://synbiohub.org/public/igem/BBa_K916000/annotation2202117/range2202117/1
type: Range
persistent identity: range2202117
display id: range2202117
version: 1
direction: direction/forward
top level: BBa_K916000
start: 1
end: 3
orientation: inline

BBa_K876038
type: ComponentDefinition
persistent identity: BBa K876038
display id: BBa_K876038
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TT-pLasR-araC
creator: Leah Ferrell, Sanket Shah, Shayan Ghassemi, Zakaraya Ashour
created: 2012-10-01T11:00:00Z
modified: 2015-05-08T01:13:38Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1138_
m user id: 0
owner id: 13835
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: TT-pLasR-araC
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K876044
component: BBa_B0015
sequence annotation: BBa_B0015
sequence annotation: BBa_K876044
sequence: https://synbiohub.org/public/igem/BBa_K876038_sequence/1

BBa_K876044
type: Component
persistent identity: component2251259
display id: component2251259
version: 1
top level: BBa_K876038
access: public
definition: https://synbiohub.org/public/igem/BBa_K876044/1

BBa_B0015
type: Component
persistent identity: component2251249
display id: component2251249
version: 1
top level: BBa_K876038
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2251249
display id: annotation2251249
version: 1
top level: BBa_K876038
location: https://synbiohub.org/public/igem/BBa_K876038/annotation2251249/range2251249/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K876038/annotation2251249/range2251249/1
type: Range
persistent identity: range2251249
display id: range2251249
version: 1
direction: direction/reverse
top level: BBa_K876038
start: 1
end: 129
orientation: inline

BBa_K876044
type: SequenceAnnotation
persistent identity: annotation2251259
display id: annotation2251259
version: 1
top level: BBa_K876038
location: https://synbiohub.org/public/igem/BBa_K876038/annotation2251259/range2251259/1
component: BBa_K876044

https://synbiohub.org/public/igem/BBa_K876038/annotation2251259/range2251259/1
type: Range
persistent identity: range2251259
display id: range2251259
version: 1
direction: direction/reverse
top level: BBa_K876038
start: 138
end: 1240
orientation: inline

BBa_K929003
type: ComponentDefinition
persistent identity: BBa K929003
display id: BBa_K929003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: modified AID with CMV, hGH-polyA and eGFP
creator: Potsdam Bioware 2012 iGEM
created: 2012-09-17T11:00:00Z
modified: 2015-05-08T01:13:46Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1194_
m user id: 0
owner id: 14457
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: The BioBrick wtAID is an extended version of the existing AID BioBrick (BBa_K929001). It is built of 3 parts: CMV promoter (BBa_I712004), superAID, eGFP (BBa_K404316) and hGH polyadenylation signal sequence (BBa_K404108). AID: AID is known to be responsible for somatic hypermutation and the class-switch recombination of immunoglobulin in B cells. This enzyme of 28 kDa originally occurs in B cells but does also show activity after transfection into CHO cells. AID induces the deamination of cytidine to uridine at actively transcribed single strand DNA. The replacement of cytidine by uridine leads to a mismatch during DNA replication and integrates a single base substitution predominantly in the immunoglobulin genes. The AID motif is naturally terminated with the Nuclear Export Sequence (NES) that causes the protein to translocate from the nucleus to the cytoplasm. Additionally, upstream, dysfunctional Nuclear Localization Sequence (NLS) is located. Due to the fact that AID mutates the actively transcribed single stranded DNA, it is supposed that the direction of the enzyme to the inside of the nucleus would improve the mutation rate. That is why we called it superAID Functional NLS sequence: This part of the BioBrick directs the expressed protein into the nucleus, where it can mutate stronger. Kozak sequence Kozak consensus sequence is added upstream of the AID mutant to express the protein stronger. CMV promoter: CMV stands for cytomegalovirus. The CMV promoter is commonly used due to its very strong activity, and effectivity in a broad range of cell types. The BioBrick is therefore improved via addition of the strong promoter. hGH polyadenylation signal sequence: Polyadenylation is a significant part for the translation and stability of mRNA. In eukaryotes, it is part of the process that produces mature messenger RNA (mRNA) for translation. It, therefore, forms part of the larger process of gene expression. hGH terminator gives a signal to start polyadenylation in t…
mutable notes:
mutable provenance: 4 parts: CMV promoter (BBa_I712004), superAID(BBa_K929001), eGFP (BBa_K404316) and hGH polyadenylation signal sequence (BBa_K404108)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: BBa_K929001
sequence annotation: part of BBa_K103001
sequence annotation: AgeI
sequence annotation: UAA
sequence annotation: BBa_I712004
sequence annotation: AUG
sequence annotation: Kozak sequence
sequence annotation: NLS sequence
sequence annotation: BBa_K404316
sequence annotation: hGH terminator
sequence annotation: BBa_K404108
sequence annotation: BBa_K929004
sequence: https://synbiohub.org/public/igem/BBa_K929003_sequence/1

BBa_K929001
type: SequenceAnnotation
persistent identity: annotation2203639
display id: annotation2203639
version: 1
top level: BBa_K929003
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K929003/annotation2203639/range2203639/1

https://synbiohub.org/public/igem/BBa_K929003/annotation2203639/range2203639/1
type: Range
persistent identity: range2203639
display id: range2203639
version: 1
direction: direction/forward
top level: BBa_K929003
start: 663
end: 1243
orientation: inline

part of BBa_K103001
type: SequenceAnnotation
persistent identity: annotation2203643
display id: annotation2203643
version: 1
top level: BBa_K929003
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K929003/annotation2203643/range2203643/1

https://synbiohub.org/public/igem/BBa_K929003/annotation2203643/range2203643/1
type: Range
persistent identity: range2203643
display id: range2203643
version: 1
direction: direction/forward
top level: BBa_K929003
start: 697
end: 1243
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2203645
display id: annotation2203645
version: 1
top level: BBa_K929003
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K929003/annotation2203645/range2203645/1

https://synbiohub.org/public/igem/BBa_K929003/annotation2203645/range2203645/1
type: Range
persistent identity: range2203645
display id: range2203645
version: 1
direction: direction/forward
top level: BBa_K929003
start: 1958
end: 1963
orientation: inline

UAA
type: SequenceAnnotation
persistent identity: annotation2203646
display id: annotation2203646
version: 1
top level: BBa_K929003
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K929003/annotation2203646/range2203646/1

https://synbiohub.org/public/igem/BBa_K929003/annotation2203646/range2203646/1
type: Range
persistent identity: range2203646
display id: range2203646
version: 1
direction: direction/forward
top level: BBa_K929003
start: 1964
end: 1966
orientation: inline

BBa_I712004
type: SequenceAnnotation
persistent identity: annotation2203637
display id: annotation2203637
version: 1
top level: BBa_K929003
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K929003/annotation2203637/range2203637/1

https://synbiohub.org/public/igem/BBa_K929003/annotation2203637/range2203637/1
type: Range
persistent identity: range2203637
display id: range2203637
version: 1
direction: direction/reverse
top level: BBa_K929003
start: 1
end: 654
orientation: inline

AUG
type: SequenceAnnotation
persistent identity: annotation2203641
display id: annotation2203641
version: 1
top level: BBa_K929003
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K929003/annotation2203641/range2203641/1

https://synbiohub.org/public/igem/BBa_K929003/annotation2203641/range2203641/1
type: Range
persistent identity: range2203641
display id: range2203641
version: 1
direction: direction/forward
top level: BBa_K929003
start: 671
end: 673
orientation: inline

Kozak sequence
type: SequenceAnnotation
persistent identity: annotation2203638
display id: annotation2203638
version: 1
top level: BBa_K929003
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K929003/annotation2203638/range2203638/1

https://synbiohub.org/public/igem/BBa_K929003/annotation2203638/range2203638/1
type: Range
persistent identity: range2203638
display id: range2203638
version: 1
direction: direction/forward
top level: BBa_K929003
start: 663
end: 677
orientation: inline

NLS sequence
type: SequenceAnnotation
persistent identity: annotation2203642
display id: annotation2203642
version: 1
top level: BBa_K929003
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K929003/annotation2203642/range2203642/1

https://synbiohub.org/public/igem/BBa_K929003/annotation2203642/range2203642/1
type: Range
persistent identity: range2203642
display id: range2203642
version: 1
direction: direction/forward
top level: BBa_K929003
start: 678
end: 696
orientation: inline

BBa_K404316
type: SequenceAnnotation
persistent identity: annotation2203644
display id: annotation2203644
version: 1
top level: BBa_K929003
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K929003/annotation2203644/range2203644/1

https://synbiohub.org/public/igem/BBa_K929003/annotation2203644/range2203644/1
type: Range
persistent identity: range2203644
display id: range2203644
version: 1
direction: direction/forward
top level: BBa_K929003
start: 1244
end: 1966
orientation: inline

hGH terminator
type: SequenceAnnotation
persistent identity: annotation2203647
display id: annotation2203647
version: 1
top level: BBa_K929003
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_K929003/annotation2203647/range2203647/1

https://synbiohub.org/public/igem/BBa_K929003/annotation2203647/range2203647/1
type: Range
persistent identity: range2203647
display id: range2203647
version: 1
direction: direction/forward
top level: BBa_K929003
start: 1975
end: 2453
orientation: inline

BBa_K404108
type: SequenceAnnotation
persistent identity: annotation2203648
display id: annotation2203648
version: 1
top level: BBa_K929003
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K929003/annotation2203648/range2203648/1

https://synbiohub.org/public/igem/BBa_K929003/annotation2203648/range2203648/1
type: Range
persistent identity: range2203648
display id: range2203648
version: 1
direction: direction/reverse
top level: BBa_K929003
start: 1975
end: 2453
orientation: inline

BBa_K929004
type: SequenceAnnotation
persistent identity: annotation2203640
display id: annotation2203640
version: 1
top level: BBa_K929003
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K929003/annotation2203640/range2203640/1

https://synbiohub.org/public/igem/BBa_K929003/annotation2203640/range2203640/1
type: Range
persistent identity: range2203640
display id: range2203640
version: 1
direction: direction/reverse
top level: BBa_K929003
start: 663
end: 1966
orientation: inline

BBa_K567001
type: ComponentDefinition
persistent identity: BBa K567001
display id: BBa_K567001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: lacI-Ptrc-tRNA(Arg)
creator: Chunying Li and Yunfeng Ruan
created: 2011-09-28T11:00:00Z
modified: 2015-05-08T01:12:43Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _735_
m user id: 0
owner id: 8782
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: The tRNA(Arg) is under the control of promoter tac. tRNA(Arg) expression is induced by 0.5mM IPTG when the OD600 of the culture reaches 0.3.
mutable notes: The correct processing to produce mature tRNA should be ensured.
mutable provenance: LacI- Ptac derived from plasmid pTrc99B. tRNA(Arg) from E.coli ArgW operon.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: tRNA-Arg
sequence annotation: PlacI
sequence annotation: lacI
sequence annotation: rrnB TE
sequence annotation: Ptrc
sequence: https://synbiohub.org/public/igem/BBa_K567001_sequence/1

tRNA-Arg
type: SequenceAnnotation
persistent identity: annotation2152784
display id: annotation2152784
version: 1
top level: BBa_K567001
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K567001/annotation2152784/range2152784/1

https://synbiohub.org/public/igem/BBa_K567001/annotation2152784/range2152784/1
type: Range
persistent identity: range2152784
display id: range2152784
version: 1
direction: direction/forward
top level: BBa_K567001
start: 1785
end: 1859
orientation: inline

PlacI
type: SequenceAnnotation
persistent identity: annotation2152781
display id: annotation2152781
version: 1
top level: BBa_K567001
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K567001/annotation2152781/range2152781/1

https://synbiohub.org/public/igem/BBa_K567001/annotation2152781/range2152781/1
type: Range
persistent identity: range2152781
display id: range2152781
version: 1
direction: direction/forward
top level: BBa_K567001
start: 331
end: 367
orientation: inline

lacI
type: SequenceAnnotation
persistent identity: annotation2152782
display id: annotation2152782
version: 1
top level: BBa_K567001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K567001/annotation2152782/range2152782/1

https://synbiohub.org/public/igem/BBa_K567001/annotation2152782/range2152782/1
type: Range
persistent identity: range2152782
display id: range2152782
version: 1
direction: direction/forward
top level: BBa_K567001
start: 397
end: 1479
orientation: inline

rrnB TE
type: SequenceAnnotation
persistent identity: annotation2152785
display id: annotation2152785
version: 1
top level: BBa_K567001
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K567001/annotation2152785/range2152785/1

https://synbiohub.org/public/igem/BBa_K567001/annotation2152785/range2152785/1
type: Range
persistent identity: range2152785
display id: range2152785
version: 1
direction: direction/forward
top level: BBa_K567001
start: 2054
end: 2133
orientation: inline

Ptrc
type: SequenceAnnotation
persistent identity: annotation2152783
display id: annotation2152783
version: 1
top level: BBa_K567001
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K567001/annotation2152783/range2152783/1

https://synbiohub.org/public/igem/BBa_K567001/annotation2152783/range2152783/1
type: Range
persistent identity: range2152783
display id: range2152783
version: 1
direction: direction/forward
top level: BBa_K567001
start: 1709
end: 1739
orientation: inline

PenI
type: ComponentDefinition
persistent identity: BBa R0074
display id: BBa_R0074
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter (PenI regulated)
creator: crackdots
created: 2004-01-27T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: PenP operator from Bacillus Lichenformis. Two operator sites are negatively regulated by PenI (C0074).
mutable notes: extends slightly past -50
mutable provenance: bacillus licheniformis
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10
sequence annotation: -35
sequence annotation: dimer left half
sequence annotation: dimer right half
sequence annotation: PenI
sequence: https://synbiohub.org/public/igem/BBa_R0074_sequence/1

-10
type: SequenceAnnotation
persistent identity: annotation319212
display id: annotation319212
version: 1
top level: PenI
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0074/annotation319212/range319212/1

https://synbiohub.org/public/igem/BBa_R0074/annotation319212/range319212/1
type: Range
persistent identity: range319212
display id: range319212
version: 1
direction: direction/forward
top level: PenI
start: 46
end: 51
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation319211
display id: annotation319211
version: 1
top level: PenI
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0074/annotation319211/range319211/1

https://synbiohub.org/public/igem/BBa_R0074/annotation319211/range319211/1
type: Range
persistent identity: range319211
display id: range319211
version: 1
direction: direction/forward
top level: PenI
start: 23
end: 28
orientation: inline

dimer left half
type: SequenceAnnotation
persistent identity: annotation319788
display id: annotation319788
version: 1
top level: PenI
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0074/annotation319788/range319788/1

https://synbiohub.org/public/igem/BBa_R0074/annotation319788/range319788/1
type: Range
persistent identity: range319788
display id: range319788
version: 1
direction: direction/forward
top level: PenI
start: 31
end: 53
orientation: inline

dimer right half
type: SequenceAnnotation
persistent identity: annotation319789
display id: annotation319789
version: 1
top level: PenI
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0074/annotation319789/range319789/1

https://synbiohub.org/public/igem/BBa_R0074/annotation319789/range319789/1
type: Range
persistent identity: range319789
display id: range319789
version: 1
direction: direction/forward
top level: PenI
start: 55
end: 76
orientation: inline

PenI
type: SequenceAnnotation
persistent identity: annotation302713
display id: annotation302713
version: 1
top level: PenI
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_R0074/annotation302713/range302713/1

https://synbiohub.org/public/igem/BBa_R0074/annotation302713/range302713/1
type: Range
persistent identity: range302713
display id: range302713
version: 1
direction: direction/forward
top level: PenI
start: 1
end: 77
orientation: inline

EYFP
type: ComponentDefinition
persistent identity: BBa E0034
display id: BBa_E0034
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: enhanced yellow fluorescent protein derived from A. victoria GFP
creator: jcbraff
created: 2004-06-06T11:00:00Z
modified: 2015-08-31T04:07:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 61
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: AAV
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_E0034_sequence/1

AAV
type: SequenceAnnotation
persistent identity: annotation1245672
display id: annotation1245672
version: 1
top level: EYFP
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_E0034/annotation1245672/range1245672/1

https://synbiohub.org/public/igem/BBa_E0034/annotation1245672/range1245672/1
type: Range
persistent identity: range1245672
display id: range1245672
version: 1
direction: direction/forward
top level: EYFP
start: 718
end: 756
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1245671
display id: annotation1245671
version: 1
top level: EYFP
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_E0034/annotation1245671/range1245671/1

https://synbiohub.org/public/igem/BBa_E0034/annotation1245671/range1245671/1
type: Range
persistent identity: range1245671
display id: range1245671
version: 1
direction: direction/forward
top level: EYFP
start: 757
end: 762
orientation: inline

R6K
type: ComponentDefinition
persistent identity: BBa J61001
display id: BBa_J61001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [R6K] Origin of replication
creator: John Anderson
created: 2006-07-30T11:00:00Z
modified: 2015-08-31T02:02:59Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: R6K origin of replication requires a pir+ or pir116 strain for replication in the absence of a second origin. In most E. coli strains, this is a silent feature. {pSB1A2-Bca1011}
mutable notes: N/A
mutable provenance: PCR ca1011F/R on pG80ko (445 bp, EcoRI/SpeI) Sub into pSB1A2-I13522 (EcoRI/SpeI) Product is Bca1011 ---- ca1011F Forward Biobricking of R6K GGACTgaattcgcggccgcttctagagtgattcgcacgggcccatg ca1011R Reverse biobricking of R6K ccgctactagtaGCAGTTCAACCTGTTGATAG
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: R6K
sequence: https://synbiohub.org/public/igem/BBa_J61001_sequence/1

R6K
type: SequenceAnnotation
persistent identity: annotation1892271
display id: annotation1892271
version: 1
top level: R6K
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J61001/annotation1892271/range1892271/1

https://synbiohub.org/public/igem/BBa_J61001/annotation1892271/range1892271/1
type: Range
persistent identity: range1892271
display id: range1892271
version: 1
direction: direction/forward
top level: R6K
start: 27
end: 406
orientation: inline

BBa_I6211
type: ComponentDefinition
persistent identity: BBa I6211
display id: BBa_I6211
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter O_H Test R0083.E0432
creator: Jennifer Braff
created: 2004-05-09T11:00:00Z
modified: 2015-08-31T04:07:44Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 60
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_B0012
component: BBa_B0010
component: BBa_R0083
component: BBa_E0032
component: BBa_B0034
sequence annotation: BBa_R0083
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_E0032
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_I6211_sequence/1

BBa_B0012
type: Component
persistent identity: component950799
display id: component950799
version: 1
top level: BBa_I6211
access: public
definition: BBa_B0012

BBa_B0010
type: Component
persistent identity: component950789
display id: component950789
version: 1
top level: BBa_I6211
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_R0083
type: Component
persistent identity: component950764
display id: component950764
version: 1
top level: BBa_I6211
access: public
definition: OmpR

BBa_E0032
type: Component
persistent identity: component950781
display id: component950781
version: 1
top level: BBa_I6211
access: public
definition: YFP

BBa_B0034
type: Component
persistent identity: component950769
display id: component950769
version: 1
top level: BBa_I6211
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0083
type: SequenceAnnotation
persistent identity: annotation950764
display id: annotation950764
version: 1
top level: BBa_I6211
location: https://synbiohub.org/public/igem/BBa_I6211/annotation950764/range950764/1
component: BBa_R0083

https://synbiohub.org/public/igem/BBa_I6211/annotation950764/range950764/1
type: Range
persistent identity: range950764
display id: range950764
version: 1
direction: direction/forward
top level: BBa_I6211
start: 1
end: 78
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation950789
display id: annotation950789
version: 1
top level: BBa_I6211
location: https://synbiohub.org/public/igem/BBa_I6211/annotation950789/range950789/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I6211/annotation950789/range950789/1
type: Range
persistent identity: range950789
display id: range950789
version: 1
direction: direction/forward
top level: BBa_I6211
start: 875
end: 954
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation950799
display id: annotation950799
version: 1
top level: BBa_I6211
location: https://synbiohub.org/public/igem/BBa_I6211/annotation950799/range950799/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I6211/annotation950799/range950799/1
type: Range
persistent identity: range950799
display id: range950799
version: 1
direction: direction/forward
top level: BBa_I6211
start: 963
end: 1003
orientation: inline

BBa_E0032
type: SequenceAnnotation
persistent identity: annotation950781
display id: annotation950781
version: 1
top level: BBa_I6211
location: https://synbiohub.org/public/igem/BBa_I6211/annotation950781/range950781/1
component: BBa_E0032

https://synbiohub.org/public/igem/BBa_I6211/annotation950781/range950781/1
type: Range
persistent identity: range950781
display id: range950781
version: 1
direction: direction/forward
top level: BBa_I6211
start: 105
end: 866
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation950769
display id: annotation950769
version: 1
top level: BBa_I6211
location: https://synbiohub.org/public/igem/BBa_I6211/annotation950769/range950769/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I6211/annotation950769/range950769/1
type: Range
persistent identity: range950769
display id: range950769
version: 1
direction: direction/forward
top level: BBa_I6211
start: 87
end: 98
orientation: inline

BBa_I718015
type: ComponentDefinition
persistent identity: BBa I718015
display id: BBa_I718015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ter-lox66-rbs-dapA (B. Subtilis)
creator: David Bikard
created: 2007-10-25T11:00:00Z
modified: 2015-08-31T04:07:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _141_
m user id: 0
owner id: 1481
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: downstream construct of the synthetic multicellular bacteria
mutable notes: none
mutable provenance: none
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_B0012
component: BBa_I718005
component: BBa_I718016
component: BBa_B0030
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_I718016
sequence annotation: BBa_B0012
sequence annotation: BBa_B0030
sequence annotation: BBa_I718005
sequence: https://synbiohub.org/public/igem/BBa_I718015_sequence/1

BBa_B0012
type: Component
persistent identity: component1956324
display id: component1956324
version: 1
top level: BBa_I718015
access: public
definition: BBa_B0012

BBa_I718005
type: Component
persistent identity: component1956335
display id: component1956335
version: 1
top level: BBa_I718015
access: public
definition: https://synbiohub.org/public/igem/BBa_I718005/1

BBa_I718016
type: Component
persistent identity: component1956328
display id: component1956328
version: 1
top level: BBa_I718015
access: public
definition: https://synbiohub.org/public/igem/BBa_I718016/1

BBa_B0030
type: Component
persistent identity: component1956330
display id: component1956330
version: 1
top level: BBa_I718015
access: public
definition: BBa_B0030

BBa_B0010
type: Component
persistent identity: component1956322
display id: component1956322
version: 1
top level: BBa_I718015
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1956322
display id: annotation1956322
version: 1
top level: BBa_I718015
location: https://synbiohub.org/public/igem/BBa_I718015/annotation1956322/range1956322/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I718015/annotation1956322/range1956322/1
type: Range
persistent identity: range1956322
display id: range1956322
version: 1
direction: direction/forward
top level: BBa_I718015
start: 1
end: 80
orientation: inline

BBa_I718016
type: SequenceAnnotation
persistent identity: annotation1956328
display id: annotation1956328
version: 1
top level: BBa_I718015
location: https://synbiohub.org/public/igem/BBa_I718015/annotation1956328/range1956328/1
component: BBa_I718016

https://synbiohub.org/public/igem/BBa_I718015/annotation1956328/range1956328/1
type: Range
persistent identity: range1956328
display id: range1956328
version: 1
direction: direction/reverse
top level: BBa_I718015
start: 138
end: 171
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1956324
display id: annotation1956324
version: 1
top level: BBa_I718015
location: https://synbiohub.org/public/igem/BBa_I718015/annotation1956324/range1956324/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I718015/annotation1956324/range1956324/1
type: Range
persistent identity: range1956324
display id: range1956324
version: 1
direction: direction/forward
top level: BBa_I718015
start: 89
end: 129
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1956330
display id: annotation1956330
version: 1
top level: BBa_I718015
location: https://synbiohub.org/public/igem/BBa_I718015/annotation1956330/range1956330/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_I718015/annotation1956330/range1956330/1
type: Range
persistent identity: range1956330
display id: range1956330
version: 1
direction: direction/forward
top level: BBa_I718015
start: 180
end: 194
orientation: inline

BBa_I718005
type: SequenceAnnotation
persistent identity: annotation1956335
display id: annotation1956335
version: 1
top level: BBa_I718015
location: https://synbiohub.org/public/igem/BBa_I718015/annotation1956335/range1956335/1
component: BBa_I718005

https://synbiohub.org/public/igem/BBa_I718015/annotation1956335/range1956335/1
type: Range
persistent identity: range1956335
display id: range1956335
version: 1
direction: direction/reverse
top level: BBa_I718015
start: 201
end: 1076
orientation: inline

BBa_J202012
type: ComponentDefinition
persistent identity: BBa J202012
display id: BBa_J202012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PBAD Mutant#13
creator: Jiyeon Park
created: 2012-07-25T11:00:00Z
modified: 2015-08-31T04:08:37Z
discontinued: false
dominant: false
group u list: _910_
m user id: 0
owner id: 11164
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is PBAD (BBa_I13453) mutant. -70 relative to the araC transcriptional start site was changed from G to A.
mutable notes: In order to regulate transcription from PBAD, araC binds three different sites called araO2, araI1, and araI2. In the absence of L-arabinose, araC binds both araO2 and araI1 and bends DNA preventing transcription from PBAD. In the presence of L-arabinose, however, araC has a conformational change because of L-arabinose and binds araI1 and araI2, enabling transcription from PBAD. Like the wild-type (I13453), this part does not have araO2 site which is essential for araC repressor function. Therefore, araC acts as an activator in the presence of L-arabinose.
mutable provenance: This mutant was created by site-directed mutagenesis.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Transcription Start
sequence annotation: araI1
sequence annotation: Mutation
sequence annotation: araI2
sequence: https://synbiohub.org/public/igem/BBa_J202012_sequence/1

Transcription Start
type: SequenceAnnotation
persistent identity: annotation2178466
display id: annotation2178466
version: 1
top level: BBa_J202012
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_J202012/annotation2178466/range2178466/1

https://synbiohub.org/public/igem/BBa_J202012/annotation2178466/range2178466/1
type: Range
persistent identity: range2178466
display id: range2178466
version: 1
direction: direction/forward
top level: BBa_J202012
start: 112
end: 112
orientation: inline

araI1
type: SequenceAnnotation
persistent identity: annotation2178467
display id: annotation2178467
version: 1
top level: BBa_J202012
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J202012/annotation2178467/range2178467/1

https://synbiohub.org/public/igem/BBa_J202012/annotation2178467/range2178467/1
type: Range
persistent identity: range2178467
display id: range2178467
version: 1
direction: direction/forward
top level: BBa_J202012
start: 40
end: 56
orientation: inline

Mutation
type: SequenceAnnotation
persistent identity: annotation2178465
display id: annotation2178465
version: 1
top level: BBa_J202012
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_J202012/annotation2178465/range2178465/1

https://synbiohub.org/public/igem/BBa_J202012/annotation2178465/range2178465/1
type: Range
persistent identity: range2178465
display id: range2178465
version: 1
direction: direction/forward
top level: BBa_J202012
start: 42
end: 42
orientation: inline

araI2
type: SequenceAnnotation
persistent identity: annotation2178468
display id: annotation2178468
version: 1
top level: BBa_J202012
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J202012/annotation2178468/range2178468/1

https://synbiohub.org/public/igem/BBa_J202012/annotation2178468/range2178468/1
type: Range
persistent identity: range2178468
display id: range2178468
version: 1
direction: direction/forward
top level: BBa_J202012
start: 61
end: 77
orientation: inline

BBa_J09855
type: ComponentDefinition
persistent identity: BBa J09855
display id: BBa_J09855
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Constitutive luxR device with pLuxR
creator: Jon Badalamenti
created: 2006-10-25T11:00:00Z
modified: 2015-08-31T04:08:21Z
discontinued: false
dominant: true
experience: experience/None
group u list: _72_40_
m user id: 0
owner id: 475
owning group id: 72
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: I14032.I0462.R0062
mutable notes: This is a universal part designed to allow induction by HSL of whatever is assembled downstream of the part.
mutable provenance: This part was constructed from registry biobricks.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_I14032
component: BBa_I0462
component: BBa_R0062
sequence annotation: BBa_I0462
sequence annotation: BBa_R0062
sequence annotation: BBa_I14032
sequence: https://synbiohub.org/public/igem/BBa_J09855_sequence/1

BBa_I14032
type: Component
persistent identity: component2260430
display id: component2260430
version: 1
top level: BBa_J09855
access: public
definition: https://synbiohub.org/public/igem/BBa_I14032/1

BBa_I0462
type: Component
persistent identity: component2260445
display id: component2260445
version: 1
top level: BBa_J09855
access: public
definition: https://synbiohub.org/public/igem/BBa_I0462/1

BBa_R0062
type: Component
persistent identity: component2260447
display id: component2260447
version: 1
top level: BBa_J09855
access: public
definition: lux pR

BBa_I0462
type: SequenceAnnotation
persistent identity: annotation2260445
display id: annotation2260445
version: 1
top level: BBa_J09855
location: https://synbiohub.org/public/igem/BBa_J09855/annotation2260445/range2260445/1
component: BBa_I0462

https://synbiohub.org/public/igem/BBa_J09855/annotation2260445/range2260445/1
type: Range
persistent identity: range2260445
display id: range2260445
version: 1
direction: direction/reverse
top level: BBa_J09855
start: 46
end: 981
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation2260447
display id: annotation2260447
version: 1
top level: BBa_J09855
location: https://synbiohub.org/public/igem/BBa_J09855/annotation2260447/range2260447/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_J09855/annotation2260447/range2260447/1
type: Range
persistent identity: range2260447
display id: range2260447
version: 1
direction: direction/forward
top level: BBa_J09855
start: 990
end: 1044
orientation: inline

BBa_I14032
type: SequenceAnnotation
persistent identity: annotation2260430
display id: annotation2260430
version: 1
top level: BBa_J09855
location: https://synbiohub.org/public/igem/BBa_J09855/annotation2260430/range2260430/1
component: BBa_I14032

https://synbiohub.org/public/igem/BBa_J09855/annotation2260430/range2260430/1
type: Range
persistent identity: range2260430
display id: range2260430
version: 1
direction: direction/reverse
top level: BBa_J09855
start: 1
end: 37
orientation: inline

BBa_K747094
type: ComponentDefinition
persistent identity: BBa K747094
display id: BBa_K747094
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_TG6_Direpeat
creator: Lucas Schneider
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:11Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: TC 6
sequence: https://synbiohub.org/public/igem/BBa_K747094_sequence/1

TC 6
type: SequenceAnnotation
persistent identity: annotation2197072
display id: annotation2197072
version: 1
top level: BBa_K747094
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747094/annotation2197072/range2197072/1

https://synbiohub.org/public/igem/BBa_K747094/annotation2197072/range2197072/1
type: Range
persistent identity: range2197072
display id: range2197072
version: 1
direction: direction/forward
top level: BBa_K747094
start: 14
end: 211
orientation: inline

BBa_K909003
type: ComponentDefinition
persistent identity: BBa K909003
display id: BBa_K909003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cph8 (Cph1-Envz-Fusionprotein) with RBS
creator: Isaak Mueller
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:44Z
discontinued: false
dominant: false
group u list: _1174_
m user id: 0
owner id: 14050
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: .
mutable notes: .
mutable provenance: .
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: BBa_K909002
sequence annotation: BBa_B0034
sequence annotation: conserved
sequence annotation: cph8 (cph1-envZ-fusion)
sequence annotation: g->a
sequence: https://synbiohub.org/public/igem/BBa_K909003_sequence/1

BBa_K909002
type: SequenceAnnotation
persistent identity: annotation2194867
display id: annotation2194867
version: 1
top level: BBa_K909003
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K909003/annotation2194867/range2194867/1

https://synbiohub.org/public/igem/BBa_K909003/annotation2194867/range2194867/1
type: Range
persistent identity: range2194867
display id: range2194867
version: 1
direction: direction/reverse
top level: BBa_K909003
start: 21
end: 2255
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2194864
display id: annotation2194864
version: 1
top level: BBa_K909003
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K909003/annotation2194864/range2194864/1

https://synbiohub.org/public/igem/BBa_K909003/annotation2194864/range2194864/1
type: Range
persistent identity: range2194864
display id: range2194864
version: 1
direction: direction/reverse
top level: BBa_K909003
start: 1
end: 12
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation2194863
display id: annotation2194863
version: 1
top level: BBa_K909003
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K909003/annotation2194863/range2194863/1

https://synbiohub.org/public/igem/BBa_K909003/annotation2194863/range2194863/1
type: Range
persistent identity: range2194863
display id: range2194863
version: 1
direction: direction/forward
top level: BBa_K909003
start: 5
end: 8
orientation: inline

cph8 (cph1-envZ-fusion)
type: SequenceAnnotation
persistent identity: annotation2194865
display id: annotation2194865
version: 1
top level: BBa_K909003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K909003/annotation2194865/range2194865/1

https://synbiohub.org/public/igem/BBa_K909003/annotation2194865/range2194865/1
type: Range
persistent identity: range2194865
display id: range2194865
version: 1
direction: direction/forward
top level: BBa_K909003
start: 21
end: 2255
orientation: inline

g->a
type: SequenceAnnotation
persistent identity: annotation2194866
display id: annotation2194866
version: 1
top level: BBa_K909003
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K909003/annotation2194866/range2194866/1

https://synbiohub.org/public/igem/BBa_K909003/annotation2194866/range2194866/1
type: Range
persistent identity: range2194866
display id: range2194866
version: 1
direction: direction/forward
top level: BBa_K909003
start: 128
end: 128
orientation: inline

TD0.01
type: ComponentDefinition
persistent identity: BBa K750012
display id: BBa_K750012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TIME DELAY0.01:LuxI(RBS0.01)->LuxR->LuxPR->GFP
creator: Sifan Wang,Ruosang Qiu,Jianzhao Chi,Muxin Yu
created: 2012-09-18T11:00:00Z
modified: 2015-05-08T01:13:12Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1001_
m user id: 0
owner id: 14212
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part contains part8 and part10. Arabinose will activate the promoter Pbad, which will cause production of protein LuxI and LuxR. The protein LuxI, LuxR and LuxPR make up the quorum sensing system, which leads to the expression of GFP.
mutable notes: editing...
mutable provenance: We built this part by biobricks from the DNA distribution kit plates 2012.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_K750007
component: BBa_K750005
sequence annotation: BBa_K750007
sequence annotation: BBa_K750005
sequence: https://synbiohub.org/public/igem/BBa_K750012_sequence/1

BBa_K750007
type: Component
persistent identity: component2312814
display id: component2312814
version: 1
top level: TD0.01
access: public
definition: https://synbiohub.org/public/igem/BBa_K750007/1

BBa_K750005
type: Component
persistent identity: component2312777
display id: component2312777
version: 1
top level: TD0.01
access: public
definition: https://synbiohub.org/public/igem/BBa_K750005/1

BBa_K750007
type: SequenceAnnotation
persistent identity: annotation2312814
display id: annotation2312814
version: 1
top level: TD0.01
location: https://synbiohub.org/public/igem/BBa_K750012/annotation2312814/range2312814/1
component: BBa_K750007

https://synbiohub.org/public/igem/BBa_K750012/annotation2312814/range2312814/1
type: Range
persistent identity: range2312814
display id: range2312814
version: 1
direction: direction/reverse
top level: TD0.01
start: 944
end: 2963
orientation: inline

BBa_K750005
type: SequenceAnnotation
persistent identity: annotation2312777
display id: annotation2312777
version: 1
top level: TD0.01
location: https://synbiohub.org/public/igem/BBa_K750012/annotation2312777/range2312777/1
component: BBa_K750005

https://synbiohub.org/public/igem/BBa_K750012/annotation2312777/range2312777/1
type: Range
persistent identity: range2312777
display id: range2312777
version: 1
direction: direction/reverse
top level: TD0.01
start: 1
end: 935
orientation: inline

BBa_K731250
type: ComponentDefinition
persistent identity: BBa K731250
display id: BBa_K731250
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Inducible araC-pBAD promoter with GFPmut3b, strong RBS
creator: Daniele Rossetto
created: 2012-08-02T11:00:00Z
modified: 2015-05-08T01:13:06Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _977_
m user id: 0
owner id: 11997
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This part has been used for testing and characterization of araC-pBAD promoter (part BBa_K731200). The part is composed of part BBa_K731200 and BBa_E0840
mutable provenance: araC-pBAD from E. coli genome, GFPmut3b is from the registry (part BBa_E0840)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_E0240
component: BBa_K731201
sequence annotation: BBa_E0240
sequence annotation: BBa_K731201
sequence: https://synbiohub.org/public/igem/BBa_K731250_sequence/1

BBa_E0240
type: Component
persistent identity: component2331165
display id: component2331165
version: 1
top level: BBa_K731250
access: public
definition: GFP report

BBa_K731201
type: Component
persistent identity: component2331154
display id: component2331154
version: 1
top level: BBa_K731250
access: public
definition: https://synbiohub.org/public/igem/BBa_K731201/1

BBa_E0240
type: SequenceAnnotation
persistent identity: annotation2331165
display id: annotation2331165
version: 1
top level: BBa_K731250
location: https://synbiohub.org/public/igem/BBa_K731250/annotation2331165/range2331165/1
component: BBa_E0240

https://synbiohub.org/public/igem/BBa_K731250/annotation2331165/range2331165/1
type: Range
persistent identity: range2331165
display id: range2331165
version: 1
direction: direction/reverse
top level: BBa_K731250
start: 1212
end: 2087
orientation: inline

BBa_K731201
type: SequenceAnnotation
persistent identity: annotation2331154
display id: annotation2331154
version: 1
top level: BBa_K731250
location: https://synbiohub.org/public/igem/BBa_K731250/annotation2331154/range2331154/1
component: BBa_K731201

https://synbiohub.org/public/igem/BBa_K731250/annotation2331154/range2331154/1
type: Range
persistent identity: range2331154
display id: range2331154
version: 1
direction: direction/reverse
top level: BBa_K731250
start: 1
end: 1203
orientation: inline

BBa_S0100
type: ComponentDefinition
persistent identity: BBa S0100
display id: BBa_S0100
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0034.C0012
creator: Caitlin Conboy and Jennifer Braff
created: 2003-12-04T12:00:00Z
modified: 2015-05-08T01:14:16Z
discontinued: false
dominant: true
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_B0034
component: BBa_C0012
sequence annotation: BBa_C0012
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_S0100_sequence/1

BBa_B0034
type: Component
persistent identity: component939698
display id: component939698
version: 1
top level: BBa_S0100
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_C0012
type: Component
persistent identity: component939708
display id: component939708
version: 1
top level: BBa_S0100
access: public
definition: lacI

BBa_C0012
type: SequenceAnnotation
persistent identity: annotation939708
display id: annotation939708
version: 1
top level: BBa_S0100
location: https://synbiohub.org/public/igem/BBa_S0100/annotation939708/range939708/1
component: BBa_C0012

https://synbiohub.org/public/igem/BBa_S0100/annotation939708/range939708/1
type: Range
persistent identity: range939708
display id: range939708
version: 1
direction: direction/forward
top level: BBa_S0100
start: 19
end: 1146
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation939698
display id: annotation939698
version: 1
top level: BBa_S0100
location: https://synbiohub.org/public/igem/BBa_S0100/annotation939698/range939698/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_S0100/annotation939698/range939698/1
type: Range
persistent identity: range939698
display id: range939698
version: 1
direction: direction/forward
top level: BBa_S0100
start: 1
end: 12
orientation: inline

BBa_K737004
type: ComponentDefinition
persistent identity: BBa K737004
display id: BBa_K737004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Inducible GVPA Generator
creator: Li Kang
created: 2012-09-17T11:00:00Z
modified: 2015-05-08T01:13:07Z
discontinued: false
dominant: false
experience: experience/None
group u list: _986_
m user id: 0
owner id: 14291
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part contains a constitutive promoter (J23106), a coding region of LacI protein with an LVA degradation tail, a terminator (B0015), a promoter repressed by LacI protein (R0011) a coding region of GvpA and GFP and a terminator (B0015) behind it. This part can be used to induce the strength of promoter (R0011) by IPTG and indicate the content of GvpA by fluorescence of GFP. In short, it can regulate the expression of GvpA. We can use different concentration of IPTG to regulate the strength of promoter (R0011) ahead GvpA Protein and GFP. And We can know the approximate concentration of GvpA by the fluorescence unit of GFP. To achieve this goal, we measured a curve to connect the concentration of IPTG and the fluorescence unit of GFP. Here is the curve:
mutable notes: We can use different concentration of IPTG to regulate the strength of promoter (R0011) ahead GvpA Protein and GFP. And We can know the approximate concentration of GvpA by the fluorescence unit of GFP. To achieve this goal, we measured a curve to connect the concentration of IPTG and the fluorescence unit of GFP.
mutable provenance: No
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_J23106
component: BBa_E0840
component: BBa_R0011
component: BBa_K737016
component: BBa_P0412
component: BBa_B0034
sequence annotation: BBa_P0412
sequence annotation: BBa_K737016
sequence annotation: BBa_B0034
sequence annotation: BBa_J23106
sequence annotation: BBa_R0011
sequence annotation: BBa_E0840
sequence: https://synbiohub.org/public/igem/BBa_K737004_sequence/1

BBa_J23106
type: Component
persistent identity: component2184807
display id: component2184807
version: 1
top level: BBa_K737004
access: public
definition: https://synbiohub.org/public/igem/BBa_J23106/1

BBa_E0840
type: Component
persistent identity: component2184839
display id: component2184839
version: 1
top level: BBa_K737004
access: public
definition: GFP genera

BBa_R0011
type: Component
persistent identity: component2184821
display id: component2184821
version: 1
top level: BBa_K737004
access: public
definition: lacI+pL

BBa_K737016
type: Component
persistent identity: component2184828
display id: component2184828
version: 1
top level: BBa_K737004
access: public
definition: https://synbiohub.org/public/igem/BBa_K737016/1

BBa_P0412
type: Component
persistent identity: component2184820
display id: component2184820
version: 1
top level: BBa_K737004
access: public
definition: https://synbiohub.org/public/igem/BBa_P0412/1

BBa_B0034
type: Component
persistent identity: component2184827
display id: component2184827
version: 1
top level: BBa_K737004
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_P0412
type: SequenceAnnotation
persistent identity: annotation2184820
display id: annotation2184820
version: 1
top level: BBa_K737004
location: https://synbiohub.org/public/igem/BBa_K737004/annotation2184820/range2184820/1
component: BBa_P0412

https://synbiohub.org/public/igem/BBa_K737004/annotation2184820/range2184820/1
type: Range
persistent identity: range2184820
display id: range2184820
version: 1
direction: direction/reverse
top level: BBa_K737004
start: 44
end: 1351
orientation: inline

BBa_K737016
type: SequenceAnnotation
persistent identity: annotation2184828
display id: annotation2184828
version: 1
top level: BBa_K737004
location: https://synbiohub.org/public/igem/BBa_K737004/annotation2184828/range2184828/1
component: BBa_K737016

https://synbiohub.org/public/igem/BBa_K737004/annotation2184828/range2184828/1
type: Range
persistent identity: range2184828
display id: range2184828
version: 1
direction: direction/reverse
top level: BBa_K737004
start: 1441
end: 1659
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2184827
display id: annotation2184827
version: 1
top level: BBa_K737004
location: https://synbiohub.org/public/igem/BBa_K737004/annotation2184827/range2184827/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K737004/annotation2184827/range2184827/1
type: Range
persistent identity: range2184827
display id: range2184827
version: 1
direction: direction/reverse
top level: BBa_K737004
start: 1423
end: 1434
orientation: inline

BBa_J23106
type: SequenceAnnotation
persistent identity: annotation2184807
display id: annotation2184807
version: 1
top level: BBa_K737004
location: https://synbiohub.org/public/igem/BBa_K737004/annotation2184807/range2184807/1
component: BBa_J23106

https://synbiohub.org/public/igem/BBa_K737004/annotation2184807/range2184807/1
type: Range
persistent identity: range2184807
display id: range2184807
version: 1
direction: direction/reverse
top level: BBa_K737004
start: 1
end: 35
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation2184821
display id: annotation2184821
version: 1
top level: BBa_K737004
location: https://synbiohub.org/public/igem/BBa_K737004/annotation2184821/range2184821/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_K737004/annotation2184821/range2184821/1
type: Range
persistent identity: range2184821
display id: range2184821
version: 1
direction: direction/forward
top level: BBa_K737004
start: 1360
end: 1413
orientation: inline

BBa_E0840
type: SequenceAnnotation
persistent identity: annotation2184839
display id: annotation2184839
version: 1
top level: BBa_K737004
location: https://synbiohub.org/public/igem/BBa_K737004/annotation2184839/range2184839/1
component: BBa_E0840

https://synbiohub.org/public/igem/BBa_K737004/annotation2184839/range2184839/1
type: Range
persistent identity: range2184839
display id: range2184839
version: 1
direction: direction/reverse
top level: BBa_K737004
start: 1668
end: 2545
orientation: inline

phaC1-A-B1
type: ComponentDefinition
persistent identity: BBa K934001
display id: BBa_K934001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: phaC1-A-B1 [P(3HB) synthesis]
creator: Taku Nakayama
created: 2012-09-16T11:00:00Z
modified: 2015-05-08T01:13:47Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1199_
m user id: 0
owner id: 14016
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: The sequence comes from Ralstonia eutropha H16 adapted to become a standard BioBrick.
mutable notes: test
mutable provenance: test
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: phaC1
sequence annotation: phaB1
sequence annotation: RBS
sequence annotation: phaA
sequence annotation: Pwt
sequence: https://synbiohub.org/public/igem/BBa_K934001_sequence/1

phaC1
type: SequenceAnnotation
persistent identity: annotation2192325
display id: annotation2192325
version: 1
top level: phaC1-A-B1
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K934001/annotation2192325/range2192325/1

https://synbiohub.org/public/igem/BBa_K934001/annotation2192325/range2192325/1
type: Range
persistent identity: range2192325
display id: range2192325
version: 1
direction: direction/forward
top level: phaC1-A-B1
start: 353
end: 2122
orientation: inline

phaB1
type: SequenceAnnotation
persistent identity: annotation2192327
display id: annotation2192327
version: 1
top level: phaC1-A-B1
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K934001/annotation2192327/range2192327/1

https://synbiohub.org/public/igem/BBa_K934001/annotation2192327/range2192327/1
type: Range
persistent identity: range2192327
display id: range2192327
version: 1
direction: direction/forward
top level: phaC1-A-B1
start: 3463
end: 4203
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2192331
display id: annotation2192331
version: 1
top level: phaC1-A-B1
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K934001/annotation2192331/range2192331/1

https://synbiohub.org/public/igem/BBa_K934001/annotation2192331/range2192331/1
type: Range
persistent identity: range2192331
display id: range2192331
version: 1
direction: direction/forward
top level: phaC1-A-B1
start: 3453
end: 3456
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2192329
display id: annotation2192329
version: 1
top level: phaC1-A-B1
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K934001/annotation2192329/range2192329/1

https://synbiohub.org/public/igem/BBa_K934001/annotation2192329/range2192329/1
type: Range
persistent identity: range2192329
display id: range2192329
version: 1
direction: direction/forward
top level: phaC1-A-B1
start: 336
end: 342
orientation: inline

phaA
type: SequenceAnnotation
persistent identity: annotation2192326
display id: annotation2192326
version: 1
top level: phaC1-A-B1
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K934001/annotation2192326/range2192326/1

https://synbiohub.org/public/igem/BBa_K934001/annotation2192326/range2192326/1
type: Range
persistent identity: range2192326
display id: range2192326
version: 1
direction: direction/forward
top level: phaC1-A-B1
start: 2207
end: 3388
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2192330
display id: annotation2192330
version: 1
top level: phaC1-A-B1
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K934001/annotation2192330/range2192330/1

https://synbiohub.org/public/igem/BBa_K934001/annotation2192330/range2192330/1
type: Range
persistent identity: range2192330
display id: range2192330
version: 1
direction: direction/forward
top level: phaC1-A-B1
start: 2196
end: 2199
orientation: inline

Pwt
type: SequenceAnnotation
persistent identity: annotation2192328
display id: annotation2192328
version: 1
top level: phaC1-A-B1
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K934001/annotation2192328/range2192328/1

https://synbiohub.org/public/igem/BBa_K934001/annotation2192328/range2192328/1
type: Range
persistent identity: range2192328
display id: range2192328
version: 1
direction: direction/forward
top level: phaC1-A-B1
start: 1
end: 50
orientation: inline

BBa_K784006
type: ComponentDefinition
persistent identity: BBa K784006
display id: BBa_K784006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Theophylline riboswitch 12.1 + mCherry
creator: Ilya Vainberg Slutskin
created: 2012-09-12T11:00:00Z
modified: 2015-05-08T01:13:21Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _1039_
m user id: 0
owner id: 11677
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part is a direct fusion of the theophylline riboswitch (BBa_K784005) and the mCherry protein (BBa_J06504) which was amplified from BBa_J06702. The part was generated by [http://openwetware.org/wiki/Assembly_pcr Assembly_pcr]. This part was to be used in order to characterize the effect of theophylline concentration on the level of translation of the mCherry protein.
mutable notes: The theophylline riboswitch had to be directly fused to the start codon of the mCherry protein. To achieve this, [http://openwetware.org/wiki/Assembly_pcr Assembly_pcr] was used.
mutable provenance: This part is a composite part of BBa_K784005 and BBa_J06504
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J06504
component: BBa_K784005
sequence annotation: BBa_J06504
sequence annotation: BBa_K784005
sequence: https://synbiohub.org/public/igem/BBa_K784006_sequence/1

BBa_J06504
type: Component
persistent identity: component2182910
display id: component2182910
version: 1
top level: BBa_K784006
access: public
definition: mCherry

BBa_K784005
type: Component
persistent identity: component2182907
display id: component2182907
version: 1
top level: BBa_K784006
access: public
definition: https://synbiohub.org/public/igem/BBa_K784005/1

BBa_J06504
type: SequenceAnnotation
persistent identity: annotation2182910
display id: annotation2182910
version: 1
top level: BBa_K784006
location: https://synbiohub.org/public/igem/BBa_K784006/annotation2182910/range2182910/1
component: BBa_J06504

https://synbiohub.org/public/igem/BBa_K784006/annotation2182910/range2182910/1
type: Range
persistent identity: range2182910
display id: range2182910
version: 1
direction: direction/reverse
top level: BBa_K784006
start: 75
end: 788
orientation: inline

BBa_K784005
type: SequenceAnnotation
persistent identity: annotation2182907
display id: annotation2182907
version: 1
top level: BBa_K784006
location: https://synbiohub.org/public/igem/BBa_K784006/annotation2182907/range2182907/1
component: BBa_K784005

https://synbiohub.org/public/igem/BBa_K784006/annotation2182907/range2182907/1
type: Range
persistent identity: range2182907
display id: range2182907
version: 1
direction: direction/reverse
top level: BBa_K784006
start: 1
end: 74
orientation: inline

BBa_J202014
type: ComponentDefinition
persistent identity: BBa J202014
display id: BBa_J202014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PBAD Mutant#15
creator: Jiyeon Park
created: 2012-07-25T11:00:00Z
modified: 2015-08-31T04:08:37Z
discontinued: false
dominant: false
group u list: _910_
m user id: 0
owner id: 11164
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is PBAD (BBa_I13453) mutant. -59 relative to the araC transcriptional start site was changed from C to T.
mutable notes: In order to regulate transcription from PBAD, araC binds three different sites called araO2, araI1, and araI2. In the absence of L-arabinose, araC binds both araO2 and araI1 and bends DNA preventing transcription from PBAD. In the presence of L-arabinose, however, araC has a conformational change because of L-arabinose and binds araI1 and araI2, enabling transcription from PBAD. Like the wild-type (I13453), this part does not have araO2 site which is essential for araC repressor function. Therefore, araC acts as an activator in the presence of L-arabinose.
mutable provenance: This mutant was created by site-directed mutagenesis.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Transcription Start
sequence annotation: araI2
sequence annotation: araI1
sequence annotation: Mutation
sequence: https://synbiohub.org/public/igem/BBa_J202014_sequence/1

Transcription Start
type: SequenceAnnotation
persistent identity: annotation2178474
display id: annotation2178474
version: 1
top level: BBa_J202014
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_J202014/annotation2178474/range2178474/1

https://synbiohub.org/public/igem/BBa_J202014/annotation2178474/range2178474/1
type: Range
persistent identity: range2178474
display id: range2178474
version: 1
direction: direction/forward
top level: BBa_J202014
start: 112
end: 112
orientation: inline

araI2
type: SequenceAnnotation
persistent identity: annotation2178476
display id: annotation2178476
version: 1
top level: BBa_J202014
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J202014/annotation2178476/range2178476/1

https://synbiohub.org/public/igem/BBa_J202014/annotation2178476/range2178476/1
type: Range
persistent identity: range2178476
display id: range2178476
version: 1
direction: direction/forward
top level: BBa_J202014
start: 61
end: 77
orientation: inline

araI1
type: SequenceAnnotation
persistent identity: annotation2178475
display id: annotation2178475
version: 1
top level: BBa_J202014
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J202014/annotation2178475/range2178475/1

https://synbiohub.org/public/igem/BBa_J202014/annotation2178475/range2178475/1
type: Range
persistent identity: range2178475
display id: range2178475
version: 1
direction: direction/forward
top level: BBa_J202014
start: 40
end: 56
orientation: inline

Mutation
type: SequenceAnnotation
persistent identity: annotation2178473
display id: annotation2178473
version: 1
top level: BBa_J202014
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_J202014/annotation2178473/range2178473/1

https://synbiohub.org/public/igem/BBa_J202014/annotation2178473/range2178473/1
type: Range
persistent identity: range2178473
display id: range2178473
version: 1
direction: direction/forward
top level: BBa_J202014
start: 53
end: 53
orientation: inline

BBa_K850000
type: ComponentDefinition
persistent identity: BBa K850000
display id: BBa_K850000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AGM1 gene from candida albicans
creator: Min-Gyu Kim
created: 2012-09-14T11:00:00Z
modified: 2015-05-08T01:13:34Z
discontinued: false
dominant: false
group u list: _1110_
m user id: 0
owner id: 10645
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Phosphoacetylglucosamine mutase (N-acetylglucosamine-phosphate mutase); enzyme of UDP-N-acetylglucosamine (UDP-GlcNAc) biosynthesis (1) This enzyme is part of the pathway to make the sugar subunits that make up the chitin polymer. This pathway was taken from yeast but has been shown to function in Acetobacter xylinum.
mutable notes: Gibson assembly fragment must have ovelapping sequences of about 50 bp.
mutable provenance: The sequence is from the candida genome database orf19.5013 (C. albicans SC5314. It contains a ribosome binding site. It was synthesized in <500bp pieces for Gibson Assembly and assembeled into pSB1C3.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K850000_sequence/1

BBa_K934023
type: ComponentDefinition
persistent identity: BBa K934023
display id: BBa_K934023
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 3OC6HSL->3OC12HSL
creator: Naoki Tsukuda
created: 2012-09-16T11:00:00Z
modified: 2015-05-08T01:13:47Z
discontinued: true
dominant: false
experience: experience/None
group u list: _1199_
m user id: 0
owner id: 14032
owning group id: 9
part status: Released HQ 2013
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: This part generates LasI(which generate 3OC12HSL) in the presence of 3OC6HSL. We constructed it with (???) and (Plux,LasI part name).
mutable notes: test
mutable provenance: test
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_S03119
component: BBa_C0078
component: BBa_K934022
sequence annotation: BBa_C0078
sequence annotation: BBa_K934022
sequence annotation: BBa_S03119
sequence: https://synbiohub.org/public/igem/BBa_K934023_sequence/1

BBa_S03119
type: Component
persistent identity: component2184114
display id: component2184114
version: 1
top level: BBa_K934023
access: public
definition: BBa_S03119

BBa_C0078
type: Component
persistent identity: component2184086
display id: component2184086
version: 1
top level: BBa_K934023
access: public
definition: https://synbiohub.org/public/igem/BBa_C0078/1

BBa_K934022
type: Component
persistent identity: component2184094
display id: component2184094
version: 1
top level: BBa_K934023
access: public
definition: https://synbiohub.org/public/igem/BBa_K934022/1

BBa_C0078
type: SequenceAnnotation
persistent identity: annotation2184086
display id: annotation2184086
version: 1
top level: BBa_K934023
location: https://synbiohub.org/public/igem/BBa_K934023/annotation2184086/range2184086/1
component: BBa_C0078

https://synbiohub.org/public/igem/BBa_K934023/annotation2184086/range2184086/1
type: Range
persistent identity: range2184086
display id: range2184086
version: 1
direction: direction/reverse
top level: BBa_K934023
start: 85
end: 726
orientation: inline

BBa_K934022
type: SequenceAnnotation
persistent identity: annotation2184094
display id: annotation2184094
version: 1
top level: BBa_K934023
location: https://synbiohub.org/public/igem/BBa_K934023/annotation2184094/range2184094/1
component: BBa_K934022

https://synbiohub.org/public/igem/BBa_K934023/annotation2184094/range2184094/1
type: Range
persistent identity: range2184094
display id: range2184094
version: 1
direction: direction/reverse
top level: BBa_K934023
start: 1
end: 798
orientation: inline

BBa_S03119
type: SequenceAnnotation
persistent identity: annotation2184114
display id: annotation2184114
version: 1
top level: BBa_K934023
location: https://synbiohub.org/public/igem/BBa_K934023/annotation2184114/range2184114/1
component: BBa_S03119

https://synbiohub.org/public/igem/BBa_K934023/annotation2184114/range2184114/1
type: Range
persistent identity: range2184114
display id: range2184114
version: 1
direction: direction/reverse
top level: BBa_K934023
start: 807
end: 1804
orientation: inline

BBa_K567018
type: ComponentDefinition
persistent identity: BBa K567018
display id: BBa_K567018
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PT7-GFP-TAG-RFP
creator: Xiaopan Ma and Xiwen Zhao
created: 2011-09-29T11:00:00Z
modified: 2015-05-08T01:12:43Z
discontinued: false
dominant: false
experience: experience/None
group u list: _735_
m user id: 0
owner id: 8782
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: GFP and RFP linked with a flexible chain and a stop codon TAG is inserted in the flexible chain. This biobrick is under the control of T7 promoter and lac operator. This part is used to testify the function of PT7-TDRS (BBa_K567011) and tRNA(Asp)-TAG (BBa_K567013).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: GFP-TAG-RFP
sequence annotation: lac operator
sequence annotation: PT7
sequence annotation: mRFP
sequence annotation: TAG
sequence annotation: T7 TE
sequence annotation: GFP
sequence annotation: rbs
sequence: https://synbiohub.org/public/igem/BBa_K567018_sequence/1

GFP-TAG-RFP
type: SequenceAnnotation
persistent identity: annotation2154491
display id: annotation2154491
version: 1
top level: BBa_K567018
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K567018/annotation2154491/range2154491/1

https://synbiohub.org/public/igem/BBa_K567018/annotation2154491/range2154491/1
type: Range
persistent identity: range2154491
display id: range2154491
version: 1
direction: direction/forward
top level: BBa_K567018
start: 89
end: 1621
orientation: inline

lac operator
type: SequenceAnnotation
persistent identity: annotation2154347
display id: annotation2154347
version: 1
top level: BBa_K567018
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K567018/annotation2154347/range2154347/1

https://synbiohub.org/public/igem/BBa_K567018/annotation2154347/range2154347/1
type: Range
persistent identity: range2154347
display id: range2154347
version: 1
direction: direction/forward
top level: BBa_K567018
start: 21
end: 44
orientation: inline

PT7
type: SequenceAnnotation
persistent identity: annotation2154346
display id: annotation2154346
version: 1
top level: BBa_K567018
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K567018/annotation2154346/range2154346/1

https://synbiohub.org/public/igem/BBa_K567018/annotation2154346/range2154346/1
type: Range
persistent identity: range2154346
display id: range2154346
version: 1
direction: direction/forward
top level: BBa_K567018
start: 2
end: 18
orientation: inline

mRFP
type: SequenceAnnotation
persistent identity: annotation2154469
display id: annotation2154469
version: 1
top level: BBa_K567018
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K567018/annotation2154469/range2154469/1

https://synbiohub.org/public/igem/BBa_K567018/annotation2154469/range2154469/1
type: Range
persistent identity: range2154469
display id: range2154469
version: 1
direction: direction/forward
top level: BBa_K567018
start: 944
end: 1621
orientation: inline

TAG
type: SequenceAnnotation
persistent identity: annotation2154444
display id: annotation2154444
version: 1
top level: BBa_K567018
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K567018/annotation2154444/range2154444/1

https://synbiohub.org/public/igem/BBa_K567018/annotation2154444/range2154444/1
type: Range
persistent identity: range2154444
display id: range2154444
version: 1
direction: direction/forward
top level: BBa_K567018
start: 926
end: 928
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation2154492
display id: annotation2154492
version: 1
top level: BBa_K567018
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K567018/annotation2154492/range2154492/1

https://synbiohub.org/public/igem/BBa_K567018/annotation2154492/range2154492/1
type: Range
persistent identity: range2154492
display id: range2154492
version: 1
direction: direction/forward
top level: BBa_K567018
start: 1712
end: 1759
orientation: inline

GFP
type: SequenceAnnotation
persistent identity: annotation2154350
display id: annotation2154350
version: 1
top level: BBa_K567018
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K567018/annotation2154350/range2154350/1

https://synbiohub.org/public/igem/BBa_K567018/annotation2154350/range2154350/1
type: Range
persistent identity: range2154350
display id: range2154350
version: 1
direction: direction/forward
top level: BBa_K567018
start: 197
end: 910
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2154349
display id: annotation2154349
version: 1
top level: BBa_K567018
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K567018/annotation2154349/range2154349/1

https://synbiohub.org/public/igem/BBa_K567018/annotation2154349/range2154349/1
type: Range
persistent identity: range2154349
display id: range2154349
version: 1
direction: direction/forward
top level: BBa_K567018
start: 75
end: 81
orientation: inline

cI+luxR
type: ComponentDefinition
persistent identity: BBa R0065
display id: BBa_R0065
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter (lambda cI and luxR regulated -- hybrid)
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: cI repressor negatively regulates this promoter and LuxR activates its transcription.The effect of cI is dominant over LuxR. This part is based on the LuxR and cI repressor regulated hybrid promoter designed and tested by Ron Weiss. It requires the binding of two cI repressor dimers for maximal repression and contains two cI repressor binding sites namely, OR1 and OR2. This promoter is leaky in the sense that 'some' transcription is seen in the absence of both cI and LuxR. LuxI cI activity of promoter + + zero + - maximum - + zero - - leaky (no quantitative information)
mutable notes: This part was designed based on the LuxR and cI repressor regulated hybrid promoter tested by Ron Weiss and the LuxR-LuxICDABE sequence annotated by Tom Knight AF170104 .
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: OR2 cI
sequence annotation: BBa_R0065
sequence annotation: -10
sequence annotation: Lux Box
sequence annotation: -35
sequence annotation: OR1 cI
sequence annotation: lux p(R) start
sequence: https://synbiohub.org/public/igem/BBa_R0065_sequence/1

OR2 cI
type: SequenceAnnotation
persistent identity: annotation1986777
display id: annotation1986777
version: 1
top level: cI+luxR
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0065/annotation1986777/range1986777/1

https://synbiohub.org/public/igem/BBa_R0065/annotation1986777/range1986777/1
type: Range
persistent identity: range1986777
display id: range1986777
version: 1
direction: direction/forward
top level: cI+luxR
start: 57
end: 73
orientation: inline

BBa_R0065
type: SequenceAnnotation
persistent identity: annotation1986774
display id: annotation1986774
version: 1
top level: cI+luxR
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_R0065/annotation1986774/range1986774/1

https://synbiohub.org/public/igem/BBa_R0065/annotation1986774/range1986774/1
type: Range
persistent identity: range1986774
display id: range1986774
version: 1
direction: direction/forward
top level: cI+luxR
start: 1
end: 97
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986776
display id: annotation1986776
version: 1
top level: cI+luxR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0065/annotation1986776/range1986776/1

https://synbiohub.org/public/igem/BBa_R0065/annotation1986776/range1986776/1
type: Range
persistent identity: range1986776
display id: range1986776
version: 1
direction: direction/forward
top level: cI+luxR
start: 47
end: 52
orientation: inline

Lux Box
type: SequenceAnnotation
persistent identity: annotation1986775
display id: annotation1986775
version: 1
top level: cI+luxR
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0065/annotation1986775/range1986775/1

https://synbiohub.org/public/igem/BBa_R0065/annotation1986775/range1986775/1
type: Range
persistent identity: range1986775
display id: range1986775
version: 1
direction: direction/forward
top level: cI+luxR
start: 6
end: 25
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1986779
display id: annotation1986779
version: 1
top level: cI+luxR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0065/annotation1986779/range1986779/1

https://synbiohub.org/public/igem/BBa_R0065/annotation1986779/range1986779/1
type: Range
persistent identity: range1986779
display id: range1986779
version: 1
direction: direction/forward
top level: cI+luxR
start: 71
end: 76
orientation: inline

OR1 cI
type: SequenceAnnotation
persistent identity: annotation1986780
display id: annotation1986780
version: 1
top level: cI+luxR
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0065/annotation1986780/range1986780/1

https://synbiohub.org/public/igem/BBa_R0065/annotation1986780/range1986780/1
type: Range
persistent identity: range1986780
display id: range1986780
version: 1
direction: direction/forward
top level: cI+luxR
start: 81
end: 97
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986781
display id: annotation1986781
version: 1
top level: cI+luxR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0065/annotation1986781/range1986781/1

https://synbiohub.org/public/igem/BBa_R0065/annotation1986781/range1986781/1
type: Range
persistent identity: range1986781
display id: range1986781
version: 1
direction: direction/forward
top level: cI+luxR
start: 94
end: 97
orientation: inline

lux p(R) start
type: SequenceAnnotation
persistent identity: annotation1986778
display id: annotation1986778
version: 1
top level: cI+luxR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0065/annotation1986778/range1986778/1

https://synbiohub.org/public/igem/BBa_R0065/annotation1986778/range1986778/1
type: Range
persistent identity: range1986778
display id: range1986778
version: 1
direction: direction/forward
top level: cI+luxR
start: 58
end: 58
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TE from coliphageT7
creator: Reshma Shetty
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Transcription terminator for the E.coli RNA polymerase.
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 V01146 .
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
direction: direction/forward
top level: BBa_B0012
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
direction: direction/forward
top level: BBa_B0012
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
direction: direction/forward
top level: BBa_B0012
start: 34
end: 34
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
direction: direction/forward
top level: BBa_B0012
start: 1
end: 41
orientation: inline

fhlA
type: ComponentDefinition
persistent identity: BBa K778001
display id: BBa_K778001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: fhlA: a transcriptional activator for enhanced hydrogen production
creator: Tomohito Minakuchi
created: 2012-09-21T11:00:00Z
modified: 2015-05-08T01:13:17Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1032_
m user id: 0
owner id: 13134
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: fhlA
mutable notes: pst1
mutable provenance: Escherichia coli (JM109)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: stop
sequence annotation: FhlA protein
sequence annotation: Illegal Pst1_site deletion (1854bp)
sequence annotation: start
sequence annotation: A to G (1906bp) (different from E. coli K-12 MG1655)
sequence: https://synbiohub.org/public/igem/BBa_K778001_sequence/1

stop
type: SequenceAnnotation
persistent identity: annotation2200694
display id: annotation2200694
version: 1
top level: fhlA
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K778001/annotation2200694/range2200694/1

https://synbiohub.org/public/igem/BBa_K778001/annotation2200694/range2200694/1
type: Range
persistent identity: range2200694
display id: range2200694
version: 1
direction: direction/forward
top level: fhlA
start: 2077
end: 2079
orientation: inline

FhlA protein
type: SequenceAnnotation
persistent identity: annotation2201429
display id: annotation2201429
version: 1
top level: fhlA
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K778001/annotation2201429/range2201429/1

https://synbiohub.org/public/igem/BBa_K778001/annotation2201429/range2201429/1
type: Range
persistent identity: range2201429
display id: range2201429
version: 1
direction: direction/forward
top level: fhlA
start: 1
end: 2079
orientation: inline

Illegal Pst1_site deletion (1854bp)
type: SequenceAnnotation
persistent identity: annotation2200692
display id: annotation2200692
version: 1
top level: fhlA
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K778001/annotation2200692/range2200692/1

https://synbiohub.org/public/igem/BBa_K778001/annotation2200692/range2200692/1
type: Range
persistent identity: range2200692
display id: range2200692
version: 1
direction: direction/forward
top level: fhlA
start: 1854
end: 1854
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2200691
display id: annotation2200691
version: 1
top level: fhlA
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K778001/annotation2200691/range2200691/1

https://synbiohub.org/public/igem/BBa_K778001/annotation2200691/range2200691/1
type: Range
persistent identity: range2200691
display id: range2200691
version: 1
direction: direction/forward
top level: fhlA
start: 1
end: 1
orientation: inline

A to G (1906bp) (different from E. coli K-12 MG1655)
type: SequenceAnnotation
persistent identity: annotation2200693
display id: annotation2200693
version: 1
top level: fhlA
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K778001/annotation2200693/range2200693/1

https://synbiohub.org/public/igem/BBa_K778001/annotation2200693/range2200693/1
type: Range
persistent identity: range2200693
display id: range2200693
version: 1
direction: direction/forward
top level: fhlA
start: 1906
end: 1906
orientation: inline

BBa_I746105
type: ComponentDefinition
persistent identity: BBa I746105
display id: BBa_I746105
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP with agr P2 promoter
creator: Zhizhen Zhao
created: 2007-09-06T11:00:00Z
modified: 2015-08-31T04:08:04Z
discontinued: false
dominant: false
group u list: _116_
m user id: 0
owner id: 2121
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is composed of a promoter and a GFP reporter gene. This can produce green fluorescence and in theory the strength of the green flourence is much stronger when there is phosphorylated AgrA in the cell. This is used to test the basal activity of agr P2 promoter.
mutable notes: NA
mutable provenance: We used two subparts from the registry to produce the new part. BBa_I746104+BBa_E0840
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0030
component: BBa_E0040
component: BBa_B0010
component: BBa_B0012
component: BBa_I746104
sequence annotation: BBa_E0040
sequence annotation: BBa_I746104
sequence annotation: BBa_B0012
sequence annotation: BBa_B0030
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_I746105_sequence/1

BBa_B0030
type: Component
persistent identity: component1943711
display id: component1943711
version: 1
top level: BBa_I746105
access: public
definition: BBa_B0030

BBa_E0040
type: Component
persistent identity: component1943714
display id: component1943714
version: 1
top level: BBa_I746105
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_B0010
type: Component
persistent identity: component1943715
display id: component1943715
version: 1
top level: BBa_I746105
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component1943717
display id: component1943717
version: 1
top level: BBa_I746105
access: public
definition: BBa_B0012

BBa_I746104
type: Component
persistent identity: component1943709
display id: component1943709
version: 1
top level: BBa_I746105
access: public
definition: BBa_I746104

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1943714
display id: annotation1943714
version: 1
top level: BBa_I746105
location: https://synbiohub.org/public/igem/BBa_I746105/annotation1943714/range1943714/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_I746105/annotation1943714/range1943714/1
type: Range
persistent identity: range1943714
display id: range1943714
version: 1
direction: direction/reverse
top level: BBa_I746105
start: 126
end: 845
orientation: inline

BBa_I746104
type: SequenceAnnotation
persistent identity: annotation1943709
display id: annotation1943709
version: 1
top level: BBa_I746105
location: https://synbiohub.org/public/igem/BBa_I746105/annotation1943709/range1943709/1
component: BBa_I746104

https://synbiohub.org/public/igem/BBa_I746105/annotation1943709/range1943709/1
type: Range
persistent identity: range1943709
display id: range1943709
version: 1
direction: direction/reverse
top level: BBa_I746105
start: 1
end: 96
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1943717
display id: annotation1943717
version: 1
top level: BBa_I746105
location: https://synbiohub.org/public/igem/BBa_I746105/annotation1943717/range1943717/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I746105/annotation1943717/range1943717/1
type: Range
persistent identity: range1943717
display id: range1943717
version: 1
direction: direction/forward
top level: BBa_I746105
start: 942
end: 982
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1943711
display id: annotation1943711
version: 1
top level: BBa_I746105
location: https://synbiohub.org/public/igem/BBa_I746105/annotation1943711/range1943711/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_I746105/annotation1943711/range1943711/1
type: Range
persistent identity: range1943711
display id: range1943711
version: 1
direction: direction/forward
top level: BBa_I746105
start: 105
end: 119
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1943715
display id: annotation1943715
version: 1
top level: BBa_I746105
location: https://synbiohub.org/public/igem/BBa_I746105/annotation1943715/range1943715/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I746105/annotation1943715/range1943715/1
type: Range
persistent identity: range1943715
display id: range1943715
version: 1
direction: direction/forward
top level: BBa_I746105
start: 854
end: 933
orientation: inline

spacer
type: ComponentDefinition
persistent identity: BBa B0040
display id: BBa_B0040
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Spacer.1 (generic)
creator: Vinay S. Mahajan, Brian Chow, Peter Carr
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Generic spacer for ensuring a 70 bp distance between the end of the suffix of the BioBrick part containing the double terminator and the prefix of the BioBrick part containing the promoter of the new gene. Please, use the AlignX function of Vector NT to check for homology with the components in your plasmid before using this spacer.
mutable notes: The size of the spacer was choosed to meet the minimum length of a sequence that can be queried using the BLAST search engine. However, subsequences of it can be used to design shorter spacers. The sequence was selected from many more sequences randomly generated using the Random DNA Generator engine; the GC% parameter used as input was 50%. The sequences were selected based on the following constraints listed in their order of importance: the absence of any putative promoter regions, a low degree of homology with the Elowitz plasmid (whose components are widely used in our designs), no homology with other E.coli sequences as shown by BLASTN search results and the presence of a number of TAA stop codons. The second constraint was the most stringent leading to the elimination of most sequences. DE made the following changes to the original sequence in order to add stop codons in the -3 frame and more in the +2 frame (note, not all of these stop codons are UAA. Thus, if used in an organism that inserts an amino acid @ UGA or UAG the obvious will occur): T->A @ 85 T->A @ 42 C->T @ 79 A->T @ 64 A->T @ 31 T->A @ 34 C->A @ 37 Also, note that the above changes further reduce (the already very weak) homology to current NCBI-stored sequences. In the process of selecting the best sequence it appeared that a good alternative sequence for a spacer would be: AGGTTCTGATATGTAACTGTGCCCAATGTCGTTAGTGACGCATACCTCTTAAGAGGCCACTGTCCTAACA. The sequence contains no putative promoters and shows moderate homology with the 5' end of the Ampicillin resistance gene. However a strong promoter sequence starts 12 bp downstream of this sequence, and therefore the sequence presented above was preferred. The sequence is compatible (does not show significant homology) with the components in the Elowitz repressilator plasmid.
mutable provenance: Randomly generated and optimized for several parameters (see Design notes).
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: Spacer-1
sequence annotation: BBa_B0040
sequence: https://synbiohub.org/public/igem/BBa_B0040_sequence/1

Spacer-1
type: SequenceAnnotation
persistent identity: annotation1721
display id: annotation1721
version: 1
top level: spacer
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_B0040/annotation1721/range1721/1

https://synbiohub.org/public/igem/BBa_B0040/annotation1721/range1721/1
type: Range
persistent identity: range1721
display id: range1721
version: 1
direction: direction/forward
top level: spacer
start: 1
end: 70
orientation: inline

BBa_B0040
type: SequenceAnnotation
persistent identity: annotation7030
display id: annotation7030
version: 1
top level: spacer
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B0040/annotation7030/range7030/1

https://synbiohub.org/public/igem/BBa_B0040/annotation7030/range7030/1
type: Range
persistent identity: range7030
display id: range7030
version: 1
direction: direction/forward
top level: spacer
start: 1
end: 70
orientation: inline

BBa_K567015
type: ComponentDefinition
persistent identity: BBa K567015
display id: BBa_K567015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PT7-metGN
creator: You Wang
created: 2011-09-28T11:00:00Z
modified: 2015-05-08T01:12:43Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _735_
m user id: 0
owner id: 8782
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: T7 promoter-metG(truncated). This biobrick is constructed by putting the truncated metG (Met-RS) under the control of T7 promoter and lac operator. We have cloned metG from E.coli and the tRNA recognition domain of metG is truncated. Kana gene with start codon substituted for CGA is used to testify the function of mutated metG. When this biobrick and metY-CGA (BBa_K567016) are co-transformed into the cell, the cells can survive on the LBKana plate.
mutable notes: overlapping PCR is used
mutable provenance: metG from E.coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: MetRS(truncated)
sequence annotation: rbs
sequence annotation: lac operator
sequence annotation: T7 promoter
sequence annotation: T7 TE
sequence: https://synbiohub.org/public/igem/BBa_K567015_sequence/1

MetRS(truncated)
type: SequenceAnnotation
persistent identity: annotation2153247
display id: annotation2153247
version: 1
top level: BBa_K567015
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K567015/annotation2153247/range2153247/1

https://synbiohub.org/public/igem/BBa_K567015/annotation2153247/range2153247/1
type: Range
persistent identity: range2153247
display id: range2153247
version: 1
direction: direction/forward
top level: BBa_K567015
start: 89
end: 1219
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2150831
display id: annotation2150831
version: 1
top level: BBa_K567015
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K567015/annotation2150831/range2150831/1

https://synbiohub.org/public/igem/BBa_K567015/annotation2150831/range2150831/1
type: Range
persistent identity: range2150831
display id: range2150831
version: 1
direction: direction/forward
top level: BBa_K567015
start: 75
end: 81
orientation: inline

lac operator
type: SequenceAnnotation
persistent identity: annotation2150830
display id: annotation2150830
version: 1
top level: BBa_K567015
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K567015/annotation2150830/range2150830/1

https://synbiohub.org/public/igem/BBa_K567015/annotation2150830/range2150830/1
type: Range
persistent identity: range2150830
display id: range2150830
version: 1
direction: direction/forward
top level: BBa_K567015
start: 21
end: 44
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2150829
display id: annotation2150829
version: 1
top level: BBa_K567015
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K567015/annotation2150829/range2150829/1

https://synbiohub.org/public/igem/BBa_K567015/annotation2150829/range2150829/1
type: Range
persistent identity: range2150829
display id: range2150829
version: 1
direction: direction/forward
top level: BBa_K567015
start: 2
end: 18
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation2153277
display id: annotation2153277
version: 1
top level: BBa_K567015
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K567015/annotation2153277/range2153277/1

https://synbiohub.org/public/igem/BBa_K567015/annotation2153277/range2153277/1
type: Range
persistent identity: range2153277
display id: range2153277
version: 1
direction: direction/forward
top level: BBa_K567015
start: 1389
end: 1436
orientation: inline

BBa_I13458
type: ComponentDefinition
persistent identity: BBa I13458
display id: BBa_I13458
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: promoter C and arabinose C operon
creator: Josh Michener
created: 2005-08-16T11:00:00Z
modified: 2015-08-31T04:07:34Z
discontinued: false
dominant: false
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: PC and AraC from the AraBAD operator. PC and AraC are on the complementary strand, reading right to left.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence: https://synbiohub.org/public/igem/BBa_I13458_sequence/1

BBa_I13013
type: ComponentDefinition
persistent identity: BBa I13013
display id: BBa_I13013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS Test R0040.B0032.E0130
creator: jasonk
created: 2004-06-30T11:00:00Z
modified: 2015-08-31T04:07:32Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Parts I13007-I130014 are RBS test constructs for RBS characterization. Each consists of Lac or Tet promoter, one of the four RBS's B0030-B0033, a YFP coding protein, and B0015 terminator.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_B0010
component: BBa_B0012
component: BBa_E0030
component: BBa_B0032
component: BBa_R0040
sequence annotation: BBa_B0032
sequence annotation: BBa_R0040
sequence annotation: BBa_E0030
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_I13013_sequence/1

BBa_B0010
type: Component
persistent identity: component946861
display id: component946861
version: 1
top level: BBa_I13013
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component946871
display id: component946871
version: 1
top level: BBa_I13013
access: public
definition: BBa_B0012

BBa_E0030
type: Component
persistent identity: component946856
display id: component946856
version: 1
top level: BBa_I13013
access: public
definition: https://synbiohub.org/public/igem/BBa_E0030/1

BBa_B0032
type: Component
persistent identity: component946853
display id: component946853
version: 1
top level: BBa_I13013
access: public
definition: BBa_B0032

BBa_R0040
type: Component
persistent identity: component946842
display id: component946842
version: 1
top level: BBa_I13013
access: public
definition: p(tetR)

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation946853
display id: annotation946853
version: 1
top level: BBa_I13013
location: https://synbiohub.org/public/igem/BBa_I13013/annotation946853/range946853/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_I13013/annotation946853/range946853/1
type: Range
persistent identity: range946853
display id: range946853
version: 1
direction: direction/forward
top level: BBa_I13013
start: 63
end: 75
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation946842
display id: annotation946842
version: 1
top level: BBa_I13013
location: https://synbiohub.org/public/igem/BBa_I13013/annotation946842/range946842/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_I13013/annotation946842/range946842/1
type: Range
persistent identity: range946842
display id: range946842
version: 1
direction: direction/forward
top level: BBa_I13013
start: 1
end: 54
orientation: inline

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation946856
display id: annotation946856
version: 1
top level: BBa_I13013
location: https://synbiohub.org/public/igem/BBa_I13013/annotation946856/range946856/1
component: BBa_E0030

https://synbiohub.org/public/igem/BBa_I13013/annotation946856/range946856/1
type: Range
persistent identity: range946856
display id: range946856
version: 1
direction: direction/forward
top level: BBa_I13013
start: 82
end: 804
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation946861
display id: annotation946861
version: 1
top level: BBa_I13013
location: https://synbiohub.org/public/igem/BBa_I13013/annotation946861/range946861/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13013/annotation946861/range946861/1
type: Range
persistent identity: range946861
display id: range946861
version: 1
direction: direction/forward
top level: BBa_I13013
start: 813
end: 892
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation946871
display id: annotation946871
version: 1
top level: BBa_I13013
location: https://synbiohub.org/public/igem/BBa_I13013/annotation946871/range946871/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13013/annotation946871/range946871/1
type: Range
persistent identity: range946871
display id: range946871
version: 1
direction: direction/forward
top level: BBa_I13013
start: 901
end: 941
orientation: inline

BBa_K876069
type: ComponentDefinition
persistent identity: BBa K876069
display id: BBa_K876069
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Term-Term-pLuxR-TetR
creator: Zakaraya Ashour, Sanket Shah
created: 2012-10-01T11:00:00Z
modified: 2015-05-08T01:13:39Z
discontinued: false
dominant: true
experience: experience/None
group u list: _1138_
m user id: 0
owner id: 13835
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: TT-pLuxR-TetR
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_B0015
component: BBa_C0040
component: BBa_R0062
sequence annotation: BBa_R0062
sequence annotation: BBa_B0015
sequence annotation: BBa_C0040
sequence: https://synbiohub.org/public/igem/BBa_K876069_sequence/1

BBa_B0015
type: Component
persistent identity: component2251320
display id: component2251320
version: 1
top level: BBa_K876069
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_C0040
type: Component
persistent identity: component2251329
display id: component2251329
version: 1
top level: BBa_K876069
access: public
definition: tetR

BBa_R0062
type: Component
persistent identity: component2251322
display id: component2251322
version: 1
top level: BBa_K876069
access: public
definition: lux pR

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation2251322
display id: annotation2251322
version: 1
top level: BBa_K876069
location: https://synbiohub.org/public/igem/BBa_K876069/annotation2251322/range2251322/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_K876069/annotation2251322/range2251322/1
type: Range
persistent identity: range2251322
display id: range2251322
version: 1
direction: direction/forward
top level: BBa_K876069
start: 138
end: 192
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2251320
display id: annotation2251320
version: 1
top level: BBa_K876069
location: https://synbiohub.org/public/igem/BBa_K876069/annotation2251320/range2251320/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K876069/annotation2251320/range2251320/1
type: Range
persistent identity: range2251320
display id: range2251320
version: 1
direction: direction/reverse
top level: BBa_K876069
start: 1
end: 129
orientation: inline

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation2251329
display id: annotation2251329
version: 1
top level: BBa_K876069
location: https://synbiohub.org/public/igem/BBa_K876069/annotation2251329/range2251329/1
component: BBa_C0040

https://synbiohub.org/public/igem/BBa_K876069/annotation2251329/range2251329/1
type: Range
persistent identity: range2251329
display id: range2251329
version: 1
direction: direction/reverse
top level: BBa_K876069
start: 199
end: 883
orientation: inline

BBa_K737007
type: ComponentDefinition
persistent identity: BBa K737007
display id: BBa_K737007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Constitutive GVPC Generator
creator: Li Kang
created: 2012-09-17T11:00:00Z
modified: 2015-05-08T01:13:07Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _986_
m user id: 0
owner id: 14291
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: "It contains a constitutive promoter, GvpC protein , GFP protein and a terminator. This part can be used as a control of (J23106+P0440+R0040+B0034+GVPC+E0840). It can express GvpC protein and GFP constantly. When it transformed with (J23106+P0412+R0011+B0034+GVPA+E0840), the bacteria may can float with GFP!"
mutable notes: This part can be used as a control of (J23106+P0440+R0040+B0034+GVPC+E0840). It can express GvpC protein and GFP constantly. When it transformed with (J23106+P0412+R0011+B0034+GVPA+E0840), the bacteria may can float with GFP.
mutable provenance: Yes.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_J23106
component: BBa_K737017
component: BBa_E0840
component: BBa_B0034
sequence annotation: BBa_J23106
sequence annotation: BBa_B0034
sequence annotation: BBa_E0840
sequence annotation: BBa_K737017
sequence: https://synbiohub.org/public/igem/BBa_K737007_sequence/1

BBa_J23106
type: Component
persistent identity: component2184890
display id: component2184890
version: 1
top level: BBa_K737007
access: public
definition: https://synbiohub.org/public/igem/BBa_J23106/1

BBa_K737017
type: Component
persistent identity: component2184893
display id: component2184893
version: 1
top level: BBa_K737007
access: public
definition: https://synbiohub.org/public/igem/BBa_K737017/1

BBa_E0840
type: Component
persistent identity: component2184904
display id: component2184904
version: 1
top level: BBa_K737007
access: public
definition: GFP genera

BBa_B0034
type: Component
persistent identity: component2184892
display id: component2184892
version: 1
top level: BBa_K737007
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_J23106
type: SequenceAnnotation
persistent identity: annotation2184890
display id: annotation2184890
version: 1
top level: BBa_K737007
location: https://synbiohub.org/public/igem/BBa_K737007/annotation2184890/range2184890/1
component: BBa_J23106

https://synbiohub.org/public/igem/BBa_K737007/annotation2184890/range2184890/1
type: Range
persistent identity: range2184890
display id: range2184890
version: 1
direction: direction/reverse
top level: BBa_K737007
start: 1
end: 35
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2184892
display id: annotation2184892
version: 1
top level: BBa_K737007
location: https://synbiohub.org/public/igem/BBa_K737007/annotation2184892/range2184892/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K737007/annotation2184892/range2184892/1
type: Range
persistent identity: range2184892
display id: range2184892
version: 1
direction: direction/reverse
top level: BBa_K737007
start: 44
end: 55
orientation: inline

BBa_E0840
type: SequenceAnnotation
persistent identity: annotation2184904
display id: annotation2184904
version: 1
top level: BBa_K737007
location: https://synbiohub.org/public/igem/BBa_K737007/annotation2184904/range2184904/1
component: BBa_E0840

https://synbiohub.org/public/igem/BBa_K737007/annotation2184904/range2184904/1
type: Range
persistent identity: range2184904
display id: range2184904
version: 1
direction: direction/reverse
top level: BBa_K737007
start: 574
end: 1451
orientation: inline

BBa_K737017
type: SequenceAnnotation
persistent identity: annotation2184893
display id: annotation2184893
version: 1
top level: BBa_K737007
location: https://synbiohub.org/public/igem/BBa_K737007/annotation2184893/range2184893/1
component: BBa_K737017

https://synbiohub.org/public/igem/BBa_K737007/annotation2184893/range2184893/1
type: Range
persistent identity: range2184893
display id: range2184893
version: 1
direction: direction/reverse
top level: BBa_K737007
start: 62
end: 565
orientation: inline

BBa_K733018
type: ComponentDefinition
persistent identity: BBa K733018
display id: BBa_K733018
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: xylR+PxylA+RBS+GFP+Double Terminator
creator: WANG, Yuqi
created: 2012-09-15T11:00:00Z
modified: 2015-05-08T01:13:07Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _979_
m user id: 0
owner id: 12444
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Not available at this moment.
mutable notes: Not available at this moment.
mutable provenance: Not available at this moment.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K733002
component: BBa_E0240
sequence annotation: BBa_K733002
sequence annotation: BBa_E0240
sequence: https://synbiohub.org/public/igem/BBa_K733018_sequence/1

BBa_K733002
type: Component
persistent identity: component2183842
display id: component2183842
version: 1
top level: BBa_K733018
access: public
definition: BBa_K733002

BBa_E0240
type: Component
persistent identity: component2183853
display id: component2183853
version: 1
top level: BBa_K733018
access: public
definition: GFP report

BBa_K733002
type: SequenceAnnotation
persistent identity: annotation2183842
display id: annotation2183842
version: 1
top level: BBa_K733018
location: https://synbiohub.org/public/igem/BBa_K733018/annotation2183842/range2183842/1
component: BBa_K733002

https://synbiohub.org/public/igem/BBa_K733018/annotation2183842/range2183842/1
type: Range
persistent identity: range2183842
display id: range2183842
version: 1
direction: direction/reverse
top level: BBa_K733018
start: 1
end: 1387
orientation: inline

BBa_E0240
type: SequenceAnnotation
persistent identity: annotation2183853
display id: annotation2183853
version: 1
top level: BBa_K733018
location: https://synbiohub.org/public/igem/BBa_K733018/annotation2183853/range2183853/1
component: BBa_E0240

https://synbiohub.org/public/igem/BBa_K733018/annotation2183853/range2183853/1
type: Range
persistent identity: range2183853
display id: range2183853
version: 1
direction: direction/reverse
top level: BBa_K733018
start: 1396
end: 2271
orientation: inline

BBa_K747095
type: ComponentDefinition
persistent identity: BBa K747095
display id: BBa_K747095
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_TT6_Direpeat
creator: Lucas Schneider
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:11Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: TT 6
sequence: https://synbiohub.org/public/igem/BBa_K747095_sequence/1

TT 6
type: SequenceAnnotation
persistent identity: annotation2197073
display id: annotation2197073
version: 1
top level: BBa_K747095
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747095/annotation2197073/range2197073/1

https://synbiohub.org/public/igem/BBa_K747095/annotation2197073/range2197073/1
type: Range
persistent identity: range2197073
display id: range2197073
version: 1
direction: direction/forward
top level: BBa_K747095
start: 14
end: 211
orientation: inline

BBa_K801070
type: ComponentDefinition
persistent identity: BBa K801070
display id: BBa_K801070
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: xanthosine methyltransferase CaXMT1-strep
creator: Roman Prechtl
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1057_
m user id: 0
owner id: 11966
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: xanthosine methyltransferase CaXMT1-strep from ''Coffea arabica'' followed by a strep-tag for purification and/or detection via western blot.
mutable notes: -
mutable provenance: http://www.ncbi.nlm.nih.gov/nuccore/AB048793 synthesized by IDT, yeast codon optimized
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: CaXMT1
sequence annotation: Consensus sequence
sequence annotation: Start codon
sequence annotation: Stop codon
sequence annotation: Strep Tag
sequence: https://synbiohub.org/public/igem/BBa_K801070_sequence/1

CaXMT1
type: SequenceAnnotation
persistent identity: annotation2198398
display id: annotation2198398
version: 1
top level: BBa_K801070
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K801070/annotation2198398/range2198398/1

https://synbiohub.org/public/igem/BBa_K801070/annotation2198398/range2198398/1
type: Range
persistent identity: range2198398
display id: range2198398
version: 1
direction: direction/forward
top level: BBa_K801070
start: 7
end: 1122
orientation: inline

Consensus sequence
type: SequenceAnnotation
persistent identity: annotation2198397
display id: annotation2198397
version: 1
top level: BBa_K801070
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801070/annotation2198397/range2198397/1

https://synbiohub.org/public/igem/BBa_K801070/annotation2198397/range2198397/1
type: Range
persistent identity: range2198397
display id: range2198397
version: 1
direction: direction/forward
top level: BBa_K801070
start: 1
end: 6
orientation: inline

Start codon
type: SequenceAnnotation
persistent identity: annotation2198399
display id: annotation2198399
version: 1
top level: BBa_K801070
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K801070/annotation2198399/range2198399/1

https://synbiohub.org/public/igem/BBa_K801070/annotation2198399/range2198399/1
type: Range
persistent identity: range2198399
display id: range2198399
version: 1
direction: direction/forward
top level: BBa_K801070
start: 7
end: 9
orientation: inline

Stop codon
type: SequenceAnnotation
persistent identity: annotation2198401
display id: annotation2198401
version: 1
top level: BBa_K801070
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K801070/annotation2198401/range2198401/1

https://synbiohub.org/public/igem/BBa_K801070/annotation2198401/range2198401/1
type: Range
persistent identity: range2198401
display id: range2198401
version: 1
direction: direction/forward
top level: BBa_K801070
start: 1153
end: 1158
orientation: inline

Strep Tag
type: SequenceAnnotation
persistent identity: annotation2198400
display id: annotation2198400
version: 1
top level: BBa_K801070
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K801070/annotation2198400/range2198400/1

https://synbiohub.org/public/igem/BBa_K801070/annotation2198400/range2198400/1
type: Range
persistent identity: range2198400
display id: range2198400
version: 1
direction: direction/forward
top level: BBa_K801070
start: 1123
end: 1152
orientation: inline

BBa_I714036
type: ComponentDefinition
persistent identity: BBa I714036
display id: BBa_I714036
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Riboregulator key 1
creator: Chunbo Lou
created: 2007-10-24T11:00:00Z
modified: 2015-08-31T04:07:47Z
discontinued: false
dominant: false
group u list: _142_
m user id: 0
owner id: 1911
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: The same as J01008
mutable notes: The Isaacs key1 riboregulator taR12 is modeled on the hok/sok post-segregational killing method of plasmid R1. It makes 25 base pairs with its counterpart, the riboregulator J01010 (aka "lock 1" or "crR12"). Isaacs documentation (see reference #1): Upon induction of the araC promoter (using 0.25% arabinose solution), the taR12 key activated the corresponding crR12 lock to express the downstream indicator, GFP, to levels of fluorescence 19-fold greater than without taR12 expression. Maximal expression was reached at 70 minutes after addition of arabinose solution, and was maintained after that point.
mutable provenance: J01008
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence: https://synbiohub.org/public/igem/BBa_I714036_sequence/1

BBa_J01141
type: ComponentDefinition
persistent identity: BBa J01141
display id: BBa_J01141
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [pLacI pL][key1]
creator: Golden Bear (Berkeley iGEM 2005)
created: 2006-04-13T11:00:00Z
modified: 2015-08-31T04:08:13Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _13_1_
m user id: 0
owner id: 612
owning group id: 13
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000834
role: partType/RNA
component: BBa_R0011
component: BBa_J01008
sequence annotation: BBa_J01008
sequence annotation: BBa_R0011
sequence: https://synbiohub.org/public/igem/BBa_J01141_sequence/1

BBa_R0011
type: Component
persistent identity: component1843682
display id: component1843682
version: 1
top level: BBa_J01141
access: public
definition: lacI+pL

BBa_J01008
type: Component
persistent identity: component1843688
display id: component1843688
version: 1
top level: BBa_J01141
access: public
definition: https://synbiohub.org/public/igem/BBa_J01008/1

BBa_J01008
type: SequenceAnnotation
persistent identity: annotation1843688
display id: annotation1843688
version: 1
top level: BBa_J01141
location: https://synbiohub.org/public/igem/BBa_J01141/annotation1843688/range1843688/1
component: BBa_J01008

https://synbiohub.org/public/igem/BBa_J01141/annotation1843688/range1843688/1
type: Range
persistent identity: range1843688
display id: range1843688
version: 1
direction: direction/reverse
top level: BBa_J01141
start: 64
end: 157
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation1843682
display id: annotation1843682
version: 1
top level: BBa_J01141
location: https://synbiohub.org/public/igem/BBa_J01141/annotation1843682/range1843682/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_J01141/annotation1843682/range1843682/1
type: Range
persistent identity: range1843682
display id: range1843682
version: 1
direction: direction/forward
top level: BBa_J01141
start: 1
end: 54
orientation: inline

BBa_K902059
type: ComponentDefinition
persistent identity: BBa K902059
display id: BBa_K902059
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: katG +LAA behind an RBS (B0034)
creator: Lisa Oberding, Ali Honarmand
created: 2012-09-30T11:00:00Z
modified: 2015-05-08T01:13:43Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1167_
m user id: 0
owner id: 13399
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: KatG +LAA behind and RBS site (B0034)
mutable notes: None
mutable provenance: Registry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_B0034
component: BBa_K137068
sequence annotation: BBa_B0034
sequence annotation: BBa_K137068
sequence: https://synbiohub.org/public/igem/BBa_K902059_sequence/1

BBa_B0034
type: Component
persistent identity: component2205168
display id: component2205168
version: 1
top level: BBa_K902059
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K137068
type: Component
persistent identity: component2205170
display id: component2205170
version: 1
top level: BBa_K902059
access: public
definition: https://synbiohub.org/public/igem/BBa_K137068/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2205168
display id: annotation2205168
version: 1
top level: BBa_K902059
location: https://synbiohub.org/public/igem/BBa_K902059/annotation2205168/range2205168/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K902059/annotation2205168/range2205168/1
type: Range
persistent identity: range2205168
display id: range2205168
version: 1
direction: direction/reverse
top level: BBa_K902059
start: 1
end: 12
orientation: inline

BBa_K137068
type: SequenceAnnotation
persistent identity: annotation2205170
display id: annotation2205170
version: 1
top level: BBa_K902059
location: https://synbiohub.org/public/igem/BBa_K902059/annotation2205170/range2205170/1
component: BBa_K137068

https://synbiohub.org/public/igem/BBa_K902059/annotation2205170/range2205170/1
type: Range
persistent identity: range2205170
display id: range2205170
version: 1
direction: direction/reverse
top level: BBa_K902059
start: 19
end: 2235
orientation: inline

ecfp
type: ComponentDefinition
persistent identity: BBa E0020
display id: BBa_E0020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: engineered cyan fluorescent protein derived from A. victoria GFP
creator: Caitlin Conboy and Jennifer Braff
created: 2004-03-02T12:00:00Z
modified: 2015-08-31T04:07:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_E0020_sequence/1

GFP report
type: ComponentDefinition
persistent identity: BBa E0240
display id: BBa_E0240
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP generator
creator: Jennifer Braff
created: 2004-10-17T11:00:00Z
modified: 2015-08-31T04:07:26Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 61
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: B0032.E0040.B0015
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_E0040
component: BBa_B0032
component: BBa_B0010
component: BBa_B0012
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_E0040
sequence annotation: BBa_B0032
sequence: https://synbiohub.org/public/igem/BBa_E0240_sequence/1

BBa_E0040
type: Component
persistent identity: component1249216
display id: component1249216
version: 1
top level: GFP report
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_B0032
type: Component
persistent identity: component1249213
display id: component1249213
version: 1
top level: GFP report
access: public
definition: BBa_B0032

BBa_B0010
type: Component
persistent identity: component1249221
display id: component1249221
version: 1
top level: GFP report
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component1249231
display id: component1249231
version: 1
top level: GFP report
access: public
definition: BBa_B0012

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1249231
display id: annotation1249231
version: 1
top level: GFP report
location: https://synbiohub.org/public/igem/BBa_E0240/annotation1249231/range1249231/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_E0240/annotation1249231/range1249231/1
type: Range
persistent identity: range1249231
display id: range1249231
version: 1
direction: direction/forward
top level: GFP report
start: 836
end: 876
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1249221
display id: annotation1249221
version: 1
top level: GFP report
location: https://synbiohub.org/public/igem/BBa_E0240/annotation1249221/range1249221/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_E0240/annotation1249221/range1249221/1
type: Range
persistent identity: range1249221
display id: range1249221
version: 1
direction: direction/forward
top level: GFP report
start: 748
end: 827
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1249216
display id: annotation1249216
version: 1
top level: GFP report
location: https://synbiohub.org/public/igem/BBa_E0240/annotation1249216/range1249216/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_E0240/annotation1249216/range1249216/1
type: Range
persistent identity: range1249216
display id: range1249216
version: 1
direction: direction/forward
top level: GFP report
start: 20
end: 739
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1249213
display id: annotation1249213
version: 1
top level: GFP report
location: https://synbiohub.org/public/igem/BBa_E0240/annotation1249213/range1249213/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_E0240/annotation1249213/range1249213/1
type: Range
persistent identity: range1249213
display id: range1249213
version: 1
direction: direction/forward
top level: GFP report
start: 1
end: 13
orientation: inline

BBa_I6044
type: ComponentDefinition
persistent identity: BBa I6044
display id: BBa_I6044
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter O_H Test R0063.E0430
creator: Caitlin Conboy and Jennifer Braff
created: 2004-03-16T12:00:00Z
modified: 2015-08-31T04:07:43Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_B0012
component: BBa_E0030
component: BBa_R0063
component: BBa_B0010
component: BBa_B0034
sequence annotation: BBa_B0010
sequence annotation: BBa_E0030
sequence annotation: BBa_R0063
sequence annotation: BBa_B0034
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_I6044_sequence/1

BBa_B0012
type: Component
persistent identity: component948596
display id: component948596
version: 1
top level: BBa_I6044
access: public
definition: BBa_B0012

BBa_E0030
type: Component
persistent identity: component948581
display id: component948581
version: 1
top level: BBa_I6044
access: public
definition: https://synbiohub.org/public/igem/BBa_E0030/1

BBa_R0063
type: Component
persistent identity: component948570
display id: component948570
version: 1
top level: BBa_I6044
access: public
definition: lux pL

BBa_B0010
type: Component
persistent identity: component948586
display id: component948586
version: 1
top level: BBa_I6044
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component948579
display id: component948579
version: 1
top level: BBa_I6044
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation948586
display id: annotation948586
version: 1
top level: BBa_I6044
location: https://synbiohub.org/public/igem/BBa_I6044/annotation948586/range948586/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I6044/annotation948586/range948586/1
type: Range
persistent identity: range948586
display id: range948586
version: 1
direction: direction/forward
top level: BBa_I6044
start: 909
end: 988
orientation: inline

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation948581
display id: annotation948581
version: 1
top level: BBa_I6044
location: https://synbiohub.org/public/igem/BBa_I6044/annotation948581/range948581/1
component: BBa_E0030

https://synbiohub.org/public/igem/BBa_I6044/annotation948581/range948581/1
type: Range
persistent identity: range948581
display id: range948581
version: 1
direction: direction/forward
top level: BBa_I6044
start: 178
end: 900
orientation: inline

BBa_R0063
type: SequenceAnnotation
persistent identity: annotation948570
display id: annotation948570
version: 1
top level: BBa_I6044
location: https://synbiohub.org/public/igem/BBa_I6044/annotation948570/range948570/1
component: BBa_R0063

https://synbiohub.org/public/igem/BBa_I6044/annotation948570/range948570/1
type: Range
persistent identity: range948570
display id: range948570
version: 1
direction: direction/forward
top level: BBa_I6044
start: 1
end: 151
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation948579
display id: annotation948579
version: 1
top level: BBa_I6044
location: https://synbiohub.org/public/igem/BBa_I6044/annotation948579/range948579/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I6044/annotation948579/range948579/1
type: Range
persistent identity: range948579
display id: range948579
version: 1
direction: direction/forward
top level: BBa_I6044
start: 160
end: 171
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation948596
display id: annotation948596
version: 1
top level: BBa_I6044
location: https://synbiohub.org/public/igem/BBa_I6044/annotation948596/range948596/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I6044/annotation948596/range948596/1
type: Range
persistent identity: range948596
display id: range948596
version: 1
direction: direction/forward
top level: BBa_I6044
start: 997
end: 1037
orientation: inline

BBa_I6031
type: ComponentDefinition
persistent identity: BBa I6031
display id: BBa_I6031
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter O_H Test R0011.E0430
creator: Caitlin Conboy and Jennifer Braff
created: 2004-03-16T12:00:00Z
modified: 2015-08-31T04:07:43Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_E0430
component: BBa_R0011
sequence annotation: BBa_E0430
sequence annotation: BBa_R0011
sequence: https://synbiohub.org/public/igem/BBa_I6031_sequence/1

BBa_E0430
type: Component
persistent identity: component2221245
display id: component2221245
version: 1
top level: BBa_I6031
access: public
definition: BBa_E0430

BBa_R0011
type: Component
persistent identity: component2221231
display id: component2221231
version: 1
top level: BBa_I6031
access: public
definition: lacI+pL

BBa_E0430
type: SequenceAnnotation
persistent identity: annotation2221245
display id: annotation2221245
version: 1
top level: BBa_I6031
location: https://synbiohub.org/public/igem/BBa_I6031/annotation2221245/range2221245/1
component: BBa_E0430

https://synbiohub.org/public/igem/BBa_I6031/annotation2221245/range2221245/1
type: Range
persistent identity: range2221245
display id: range2221245
version: 1
direction: direction/reverse
top level: BBa_I6031
start: 64
end: 941
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation2221231
display id: annotation2221231
version: 1
top level: BBa_I6031
location: https://synbiohub.org/public/igem/BBa_I6031/annotation2221231/range2221231/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_I6031/annotation2221231/range2221231/1
type: Range
persistent identity: range2221231
display id: range2221231
version: 1
direction: direction/forward
top level: BBa_I6031
start: 1
end: 54
orientation: inline

BBa_K784014
type: ComponentDefinition
persistent identity: BBa K784014
display id: BBa_K784014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pLux+Spacer2+RBS+mCherry
creator: Ilya Vainberg Slutskin
created: 2012-09-15T11:00:00Z
modified: 2015-05-08T01:13:21Z
discontinued: false
dominant: false
group u list: _1039_
m user id: 0
owner id: 11677
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This sequence is the product of restriction cloning of the spacer2+RBS+mCherry downstream to the pLux promoter. In the presence of LuxR transcription can be induced by 3OC6HSL.
mutable notes: No design considerations.
mutable provenance: Cloning of the spacer2+RBS+mCherry downstream to the pLux promoter.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_R0062
component: BBa_K784009
sequence annotation: BBa_R0062
sequence annotation: BBa_K784009
sequence: https://synbiohub.org/public/igem/BBa_K784014_sequence/1

BBa_R0062
type: Component
persistent identity: component2183696
display id: component2183696
version: 1
top level: BBa_K784014
access: public
definition: lux pR

BBa_K784009
type: Component
persistent identity: component2183708
display id: component2183708
version: 1
top level: BBa_K784014
access: public
definition: https://synbiohub.org/public/igem/BBa_K784009/1

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation2183696
display id: annotation2183696
version: 1
top level: BBa_K784014
location: https://synbiohub.org/public/igem/BBa_K784014/annotation2183696/range2183696/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_K784014/annotation2183696/range2183696/1
type: Range
persistent identity: range2183696
display id: range2183696
version: 1
direction: direction/forward
top level: BBa_K784014
start: 1
end: 55
orientation: inline

BBa_K784009
type: SequenceAnnotation
persistent identity: annotation2183708
display id: annotation2183708
version: 1
top level: BBa_K784014
location: https://synbiohub.org/public/igem/BBa_K784014/annotation2183708/range2183708/1
component: BBa_K784009

https://synbiohub.org/public/igem/BBa_K784014/annotation2183708/range2183708/1
type: Range
persistent identity: range2183708
display id: range2183708
version: 1
direction: direction/reverse
top level: BBa_K784014
start: 64
end: 828
orientation: inline

RO
type: ComponentDefinition
persistent identity: BBa K838001
display id: BBa_K838001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LovTAP readout
creator: Nicolas Gobet
created: 2012-09-21T11:00:00Z
modified: 2015-05-08T01:13:33Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _1097_
m user id: 0
owner id: 12458
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Expresses dsRed
mutable notes: Has 2 SpeI and 1 EcoRI site init
mutable provenance: Nicolas Gobet
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K838001_sequence/1

BBa_I6084
type: ComponentDefinition
persistent identity: BBa I6084
display id: BBa_I6084
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter O_H Test R1053.E0432
creator: Caitlin Conboy and Jennifer Braff
created: 2004-03-17T12:00:00Z
modified: 2015-08-31T04:07:43Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_B0034
component: BBa_E0032
component: BBa_R1053
component: BBa_B0010
component: BBa_B0012
sequence annotation: BBa_E0032
sequence annotation: BBa_B0034
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_R1053
sequence: https://synbiohub.org/public/igem/BBa_I6084_sequence/1

BBa_B0034
type: Component
persistent identity: component949552
display id: component949552
version: 1
top level: BBa_I6084
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_E0032
type: Component
persistent identity: component949564
display id: component949564
version: 1
top level: BBa_I6084
access: public
definition: YFP

BBa_R1053
type: Component
persistent identity: component949542
display id: component949542
version: 1
top level: BBa_I6084
access: public
definition: https://synbiohub.org/public/igem/BBa_R1053/1

BBa_B0010
type: Component
persistent identity: component949572
display id: component949572
version: 1
top level: BBa_I6084
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component949582
display id: component949582
version: 1
top level: BBa_I6084
access: public
definition: BBa_B0012

BBa_E0032
type: SequenceAnnotation
persistent identity: annotation949564
display id: annotation949564
version: 1
top level: BBa_I6084
location: https://synbiohub.org/public/igem/BBa_I6084/annotation949564/range949564/1
component: BBa_E0032

https://synbiohub.org/public/igem/BBa_I6084/annotation949564/range949564/1
type: Range
persistent identity: range949564
display id: range949564
version: 1
direction: direction/forward
top level: BBa_I6084
start: 82
end: 843
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation949552
display id: annotation949552
version: 1
top level: BBa_I6084
location: https://synbiohub.org/public/igem/BBa_I6084/annotation949552/range949552/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I6084/annotation949552/range949552/1
type: Range
persistent identity: range949552
display id: range949552
version: 1
direction: direction/forward
top level: BBa_I6084
start: 64
end: 75
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation949572
display id: annotation949572
version: 1
top level: BBa_I6084
location: https://synbiohub.org/public/igem/BBa_I6084/annotation949572/range949572/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I6084/annotation949572/range949572/1
type: Range
persistent identity: range949572
display id: range949572
version: 1
direction: direction/forward
top level: BBa_I6084
start: 852
end: 931
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation949582
display id: annotation949582
version: 1
top level: BBa_I6084
location: https://synbiohub.org/public/igem/BBa_I6084/annotation949582/range949582/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I6084/annotation949582/range949582/1
type: Range
persistent identity: range949582
display id: range949582
version: 1
direction: direction/forward
top level: BBa_I6084
start: 940
end: 980
orientation: inline

BBa_R1053
type: SequenceAnnotation
persistent identity: annotation949542
display id: annotation949542
version: 1
top level: BBa_I6084
location: https://synbiohub.org/public/igem/BBa_I6084/annotation949542/range949542/1
component: BBa_R1053

https://synbiohub.org/public/igem/BBa_I6084/annotation949542/range949542/1
type: Range
persistent identity: range949542
display id: range949542
version: 1
direction: direction/forward
top level: BBa_I6084
start: 1
end: 55
orientation: inline

BBa_K808034
type: ComponentDefinition
persistent identity: BBa K808034
display id: BBa_K808034
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: araC-Pbad regulated terephtalate periplasmatic binding proteine of the tripartite transporter family
creator: Valentina Herbring, Sebastian Palluk, Andreas Schmidt
created: 2012-09-05T11:00:00Z
modified: 2015-05-08T01:13:26Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1065_
m user id: 0
owner id: 12529
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J61101
component: BBa_K808001
component: BBa_K808000
sequence annotation: BBa_K808000
sequence annotation: BBa_K808001
sequence annotation: BBa_J61101
sequence: https://synbiohub.org/public/igem/BBa_K808034_sequence/1

BBa_J61101
type: Component
persistent identity: component2201272
display id: component2201272
version: 1
top level: BBa_K808034
access: public
definition: https://synbiohub.org/public/igem/BBa_J61101/1

BBa_K808001
type: Component
persistent identity: component2201273
display id: component2201273
version: 1
top level: BBa_K808034
access: public
definition: https://synbiohub.org/public/igem/BBa_K808001/1

BBa_K808000
type: Component
persistent identity: component2201271
display id: component2201271
version: 1
top level: BBa_K808034
access: public
definition: Ara

BBa_K808000
type: SequenceAnnotation
persistent identity: annotation2201271
display id: annotation2201271
version: 1
top level: BBa_K808034
location: https://synbiohub.org/public/igem/BBa_K808034/annotation2201271/range2201271/1
component: BBa_K808000

https://synbiohub.org/public/igem/BBa_K808034/annotation2201271/range2201271/1
type: Range
persistent identity: range2201271
display id: range2201271
version: 1
direction: direction/reverse
top level: BBa_K808034
start: 1
end: 1209
orientation: inline

BBa_K808001
type: SequenceAnnotation
persistent identity: annotation2201273
display id: annotation2201273
version: 1
top level: BBa_K808034
location: https://synbiohub.org/public/igem/BBa_K808034/annotation2201273/range2201273/1
component: BBa_K808001

https://synbiohub.org/public/igem/BBa_K808034/annotation2201273/range2201273/1
type: Range
persistent identity: range2201273
display id: range2201273
version: 1
direction: direction/reverse
top level: BBa_K808034
start: 1236
end: 2204
orientation: inline

BBa_J61101
type: SequenceAnnotation
persistent identity: annotation2201272
display id: annotation2201272
version: 1
top level: BBa_K808034
location: https://synbiohub.org/public/igem/BBa_K808034/annotation2201272/range2201272/1
component: BBa_J61101

https://synbiohub.org/public/igem/BBa_K808034/annotation2201272/range2201272/1
type: Range
persistent identity: range2201272
display id: range2201272
version: 1
direction: direction/reverse
top level: BBa_K808034
start: 1218
end: 1229
orientation: inline

BBa_K876068
type: ComponentDefinition
persistent identity: BBa K876068
display id: BBa_K876068
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Term-Term-pLsrA-LacI
creator: Zakaraya Ashour, Sanket Shah
created: 2012-10-01T11:00:00Z
modified: 2015-05-08T01:13:39Z
discontinued: false
dominant: true
experience: experience/None
group u list: _1138_
m user id: 0
owner id: 13835
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: TT-pLasrA-LacI
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_K117002
component: BBa_B0015
component: BBa_C0012
sequence annotation: BBa_K117002
sequence annotation: BBa_B0015
sequence annotation: BBa_C0012
sequence: https://synbiohub.org/public/igem/BBa_K876068_sequence/1

BBa_K117002
type: Component
persistent identity: component2251337
display id: component2251337
version: 1
top level: BBa_K876068
access: public
definition: https://synbiohub.org/public/igem/BBa_K117002/1

BBa_B0015
type: Component
persistent identity: component2251336
display id: component2251336
version: 1
top level: BBa_K876068
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_C0012
type: Component
persistent identity: component2251339
display id: component2251339
version: 1
top level: BBa_K876068
access: public
definition: lacI

BBa_K117002
type: SequenceAnnotation
persistent identity: annotation2251337
display id: annotation2251337
version: 1
top level: BBa_K876068
location: https://synbiohub.org/public/igem/BBa_K876068/annotation2251337/range2251337/1
component: BBa_K117002

https://synbiohub.org/public/igem/BBa_K876068/annotation2251337/range2251337/1
type: Range
persistent identity: range2251337
display id: range2251337
version: 1
direction: direction/reverse
top level: BBa_K876068
start: 138
end: 239
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2251336
display id: annotation2251336
version: 1
top level: BBa_K876068
location: https://synbiohub.org/public/igem/BBa_K876068/annotation2251336/range2251336/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K876068/annotation2251336/range2251336/1
type: Range
persistent identity: range2251336
display id: range2251336
version: 1
direction: direction/reverse
top level: BBa_K876068
start: 1
end: 129
orientation: inline

BBa_C0012
type: SequenceAnnotation
persistent identity: annotation2251339
display id: annotation2251339
version: 1
top level: BBa_K876068
location: https://synbiohub.org/public/igem/BBa_K876068/annotation2251339/range2251339/1
component: BBa_C0012

https://synbiohub.org/public/igem/BBa_K876068/annotation2251339/range2251339/1
type: Range
persistent identity: range2251339
display id: range2251339
version: 1
direction: direction/forward
top level: BBa_K876068
start: 246
end: 1373
orientation: inline

BBa_K801072
type: ComponentDefinition
persistent identity: BBa K801072
display id: BBa_K801072
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 3,7-dimethylxanthine N-methyltransferase CaDXMT1-strep
creator: Roman Prechtl
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1057_
m user id: 0
owner id: 11966
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: 3,7-dimethylxanthine N-methyltransferase CaDXMT1 from ''Coffea arabica'' followed by a strep-tag for purification and/or detection via western blot.
mutable notes: -
mutable provenance: http://www.ncbi.nlm.nih.gov/nuccore/AB084125 synthesized by IDT, yeast codon optimized
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Consensus sequence
sequence annotation: Stop codon
sequence annotation: Strep Tag
sequence annotation: CaDXMT1
sequence annotation: Start codon
sequence: https://synbiohub.org/public/igem/BBa_K801072_sequence/1

Consensus sequence
type: SequenceAnnotation
persistent identity: annotation2197893
display id: annotation2197893
version: 1
top level: BBa_K801072
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801072/annotation2197893/range2197893/1

https://synbiohub.org/public/igem/BBa_K801072/annotation2197893/range2197893/1
type: Range
persistent identity: range2197893
display id: range2197893
version: 1
direction: direction/forward
top level: BBa_K801072
start: 1
end: 6
orientation: inline

Stop codon
type: SequenceAnnotation
persistent identity: annotation2198588
display id: annotation2198588
version: 1
top level: BBa_K801072
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K801072/annotation2198588/range2198588/1

https://synbiohub.org/public/igem/BBa_K801072/annotation2198588/range2198588/1
type: Range
persistent identity: range2198588
display id: range2198588
version: 1
direction: direction/forward
top level: BBa_K801072
start: 1189
end: 1194
orientation: inline

Strep Tag
type: SequenceAnnotation
persistent identity: annotation2198187
display id: annotation2198187
version: 1
top level: BBa_K801072
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K801072/annotation2198187/range2198187/1

https://synbiohub.org/public/igem/BBa_K801072/annotation2198187/range2198187/1
type: Range
persistent identity: range2198187
display id: range2198187
version: 1
direction: direction/forward
top level: BBa_K801072
start: 1159
end: 1188
orientation: inline

CaDXMT1
type: SequenceAnnotation
persistent identity: annotation2197894
display id: annotation2197894
version: 1
top level: BBa_K801072
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K801072/annotation2197894/range2197894/1

https://synbiohub.org/public/igem/BBa_K801072/annotation2197894/range2197894/1
type: Range
persistent identity: range2197894
display id: range2197894
version: 1
direction: direction/forward
top level: BBa_K801072
start: 7
end: 1158
orientation: inline

Start codon
type: SequenceAnnotation
persistent identity: annotation2198600
display id: annotation2198600
version: 1
top level: BBa_K801072
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K801072/annotation2198600/range2198600/1

https://synbiohub.org/public/igem/BBa_K801072/annotation2198600/range2198600/1
type: Range
persistent identity: range2198600
display id: range2198600
version: 1
direction: direction/forward
top level: BBa_K801072
start: 7
end: 9
orientation: inline

BBa_K876015
type: ComponentDefinition
persistent identity: BBa K876015
display id: BBa_K876015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pTet-LasR-DT
creator: Timothy Cogan
created: 2012-09-29T11:00:00Z
modified: 2015-05-08T01:13:38Z
discontinued: false
dominant: true
group u list: _1138_
m user id: 0
owner id: 13836
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Constitutive promoter for LasR which can be repressed by TetR
mutable notes: Biobrick placement
mutable provenance: Created from biobricks
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_R0040
component: BBa_C0079
component: BBa_B0015
sequence annotation: BBa_B0015
sequence annotation: BBa_R0040
sequence annotation: BBa_C0079
sequence: https://synbiohub.org/public/igem/BBa_K876015_sequence/1

BBa_R0040
type: Component
persistent identity: component2251226
display id: component2251226
version: 1
top level: BBa_K876015
access: public
definition: p(tetR)

BBa_C0079
type: Component
persistent identity: component2251235
display id: component2251235
version: 1
top level: BBa_K876015
access: public
definition: https://synbiohub.org/public/igem/BBa_C0079/1

BBa_B0015
type: Component
persistent identity: component2251242
display id: component2251242
version: 1
top level: BBa_K876015
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2251242
display id: annotation2251242
version: 1
top level: BBa_K876015
location: https://synbiohub.org/public/igem/BBa_K876015/annotation2251242/range2251242/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K876015/annotation2251242/range2251242/1
type: Range
persistent identity: range2251242
display id: range2251242
version: 1
direction: direction/reverse
top level: BBa_K876015
start: 850
end: 978
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2251226
display id: annotation2251226
version: 1
top level: BBa_K876015
location: https://synbiohub.org/public/igem/BBa_K876015/annotation2251226/range2251226/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K876015/annotation2251226/range2251226/1
type: Range
persistent identity: range2251226
display id: range2251226
version: 1
direction: direction/forward
top level: BBa_K876015
start: 1
end: 54
orientation: inline

BBa_C0079
type: SequenceAnnotation
persistent identity: annotation2251235
display id: annotation2251235
version: 1
top level: BBa_K876015
location: https://synbiohub.org/public/igem/BBa_K876015/annotation2251235/range2251235/1
component: BBa_C0079

https://synbiohub.org/public/igem/BBa_K876015/annotation2251235/range2251235/1
type: Range
persistent identity: range2251235
display id: range2251235
version: 1
direction: direction/reverse
top level: BBa_K876015
start: 61
end: 841
orientation: inline

BBa_I732074
type: ComponentDefinition
persistent identity: BBa I732074
display id: BBa_I732074
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Adaptor + LacZ-alpha Reporter
creator: Zhan Jian
created: 2007-08-01T11:00:00Z
modified: 2015-08-31T04:07:57Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_I732006
component: BBa_B0034
component: BBa_I732007
sequence annotation: BBa_I732006
sequence annotation: BBa_I732007
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_I732074_sequence/1

BBa_I732006
type: Component
persistent identity: component1940660
display id: component1940660
version: 1
top level: BBa_I732074
access: public
definition: https://synbiohub.org/public/igem/BBa_I732006/1

BBa_B0034
type: Component
persistent identity: component1940656
display id: component1940656
version: 1
top level: BBa_I732074
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_I732007
type: Component
persistent identity: component1940654
display id: component1940654
version: 1
top level: BBa_I732074
access: public
definition: Adapter

BBa_I732006
type: SequenceAnnotation
persistent identity: annotation1940660
display id: annotation1940660
version: 1
top level: BBa_I732074
location: https://synbiohub.org/public/igem/BBa_I732074/annotation1940660/range1940660/1
component: BBa_I732006

https://synbiohub.org/public/igem/BBa_I732074/annotation1940660/range1940660/1
type: Range
persistent identity: range1940660
display id: range1940660
version: 1
direction: direction/reverse
top level: BBa_I732074
start: 46
end: 279
orientation: inline

BBa_I732007
type: SequenceAnnotation
persistent identity: annotation1940654
display id: annotation1940654
version: 1
top level: BBa_I732074
location: https://synbiohub.org/public/igem/BBa_I732074/annotation1940654/range1940654/1
component: BBa_I732007

https://synbiohub.org/public/igem/BBa_I732074/annotation1940654/range1940654/1
type: Range
persistent identity: range1940654
display id: range1940654
version: 1
direction: direction/reverse
top level: BBa_I732074
start: 1
end: 19
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1940656
display id: annotation1940656
version: 1
top level: BBa_I732074
location: https://synbiohub.org/public/igem/BBa_I732074/annotation1940656/range1940656/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I732074/annotation1940656/range1940656/1
type: Range
persistent identity: range1940656
display id: range1940656
version: 1
direction: direction/reverse
top level: BBa_I732074
start: 28
end: 39
orientation: inline

BBa_K779504
type: ComponentDefinition
persistent identity: BBa K779504
display id: BBa_K779504
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Long scrambled input strand MammoBlock
creator: Kristjan Eerik Kaseniit
created: 2012-09-28T11:00:00Z
modified: 2015-05-08T01:13:18Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1033_
m user id: 0
owner id: 12684
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Long non-matching (scrambled) input strand to act as a negative control to the reporter formed by parts Part:BBa K779500 and Part:BBa K779502 or Part:BBa K779501 and Part:BBa K779502. Modifications: 5' IRD800 fluorophore, 3' phosphate. IDT DNA oligo order: /5IRD800/mAmC mUmAmA mCmCmU mAmAmC mUmAmC mAmAmC mAmAmC mAmCmU mAmUmC mCmA/3Phos/
mutable notes: In the domain, uses the same nucleotides as the Sa, but is scrambled so that the Sa and Sc domains are orthogonal. See more details at the MIT iGEM wiki: http://2012.igem.org/Team:MIT
mutable provenance: De novo design.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence annotation: Domain Sc
sequence annotation: Toehold
sequence: https://synbiohub.org/public/igem/BBa_K779504_sequence/1

Domain Sc
type: SequenceAnnotation
persistent identity: annotation2204257
display id: annotation2204257
version: 1
top level: BBa_K779504
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K779504/annotation2204257/range2204257/1

https://synbiohub.org/public/igem/BBa_K779504/annotation2204257/range2204257/1
type: Range
persistent identity: range2204257
display id: range2204257
version: 1
direction: direction/forward
top level: BBa_K779504
start: 1
end: 20
orientation: inline

Toehold
type: SequenceAnnotation
persistent identity: annotation2204258
display id: annotation2204258
version: 1
top level: BBa_K779504
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K779504/annotation2204258/range2204258/1

https://synbiohub.org/public/igem/BBa_K779504/annotation2204258/range2204258/1
type: Range
persistent identity: range2204258
display id: range2204258
version: 1
direction: direction/forward
top level: BBa_K779504
start: 21
end: 28
orientation: inline

ECFP
type: ComponentDefinition
persistent identity: BBa E0022
display id: BBa_E0022
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: enhanced cyan fluorescent protein derived from A. victoria GFP
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:25Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Cyan fluorescent protein (ECFP) reporter coding sequence without the Ribosome Binding Site. Modified with an LVA tail for rapid degradation of the protein and faster fall time for the emission.
mutable notes: BBa_E0022 cyan fluorescent protein is based on BioBrick part BBa_E0021. It has been modified to include a rapid degradation LVA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.
mutable provenance: Modified from <bb_part>BBa_E0021</bb_part>.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: CFP (LVA)
sequence annotation: SsrA
sequence annotation: BBa_E0022
sequence annotation: A
sequence annotation: 2
sequence: https://synbiohub.org/public/igem/BBa_E0022_sequence/1

CFP (LVA)
type: SequenceAnnotation
persistent identity: annotation2150
display id: annotation2150
version: 1
top level: ECFP
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_E0022/annotation2150/range2150/1

https://synbiohub.org/public/igem/BBa_E0022/annotation2150/range2150/1
type: Range
persistent identity: range2150
display id: range2150
version: 1
direction: direction/forward
top level: ECFP
start: 1
end: 762
orientation: inline

SsrA
type: SequenceAnnotation
persistent identity: annotation2153
display id: annotation2153
version: 1
top level: ECFP
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_E0022/annotation2153/range2153/1

https://synbiohub.org/public/igem/BBa_E0022/annotation2153/range2153/1
type: Range
persistent identity: range2153
display id: range2153
version: 1
direction: direction/forward
top level: ECFP
start: 719
end: 756
orientation: inline

BBa_E0022
type: SequenceAnnotation
persistent identity: annotation7040
display id: annotation7040
version: 1
top level: ECFP
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_E0022/annotation7040/range7040/1

https://synbiohub.org/public/igem/BBa_E0022/annotation7040/range7040/1
type: Range
persistent identity: range7040
display id: range7040
version: 1
direction: direction/forward
top level: ECFP
start: 1
end: 762
orientation: inline

A
type: SequenceAnnotation
persistent identity: annotation2155
display id: annotation2155
version: 1
top level: ECFP
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_E0022/annotation2155/range2155/1

https://synbiohub.org/public/igem/BBa_E0022/annotation2155/range2155/1
type: Range
persistent identity: range2155
display id: range2155
version: 1
direction: direction/forward
top level: ECFP
start: 69
end: 69
orientation: inline

2
type: SequenceAnnotation
persistent identity: annotation2154
display id: annotation2154
version: 1
top level: ECFP
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_E0022/annotation2154/range2154/1

https://synbiohub.org/public/igem/BBa_E0022/annotation2154/range2154/1
type: Range
persistent identity: range2154
display id: range2154
version: 1
direction: direction/forward
top level: ECFP
start: 757
end: 762
orientation: inline

BBa_K773002
type: ComponentDefinition
persistent identity: BBa K773002
display id: BBa_K773002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Proteorhodopsin
creator: Katie Knister
created: 2012-09-30T11:00:00Z
modified: 2015-05-08T01:13:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1025_
m user id: 0
owner id: 11646
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part carries the gene for a proteorhodopsin pump, which can generate a proton motive force for making ATP.
mutable notes: We codon optimized the gene for E. coli and eliminated four restriction sites.
mutable provenance: Synthesized with gene assembly
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: stop
sequence annotation: Proteorhodopsin
sequence annotation: Start
sequence: https://synbiohub.org/public/igem/BBa_K773002_sequence/1

stop
type: SequenceAnnotation
persistent identity: annotation2209516
display id: annotation2209516
version: 1
top level: BBa_K773002
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K773002/annotation2209516/range2209516/1

https://synbiohub.org/public/igem/BBa_K773002/annotation2209516/range2209516/1
type: Range
persistent identity: range2209516
display id: range2209516
version: 1
direction: direction/forward
top level: BBa_K773002
start: 775
end: 777
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2209517
display id: annotation2209517
version: 1
top level: BBa_K773002
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K773002/annotation2209517/range2209517/1

https://synbiohub.org/public/igem/BBa_K773002/annotation2209517/range2209517/1
type: Range
persistent identity: range2209517
display id: range2209517
version: 1
direction: direction/forward
top level: BBa_K773002
start: 772
end: 774
orientation: inline

Proteorhodopsin
type: SequenceAnnotation
persistent identity: annotation2209514
display id: annotation2209514
version: 1
top level: BBa_K773002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K773002/annotation2209514/range2209514/1

https://synbiohub.org/public/igem/BBa_K773002/annotation2209514/range2209514/1
type: Range
persistent identity: range2209514
display id: range2209514
version: 1
direction: direction/forward
top level: BBa_K773002
start: 1
end: 777
orientation: inline

Start
type: SequenceAnnotation
persistent identity: annotation2209515
display id: annotation2209515
version: 1
top level: BBa_K773002
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K773002/annotation2209515/range2209515/1

https://synbiohub.org/public/igem/BBa_K773002/annotation2209515/range2209515/1
type: Range
persistent identity: range2209515
display id: range2209515
version: 1
direction: direction/forward
top level: BBa_K773002
start: 1
end: 3
orientation: inline

BBa_I732807
type: ComponentDefinition
persistent identity: BBa I732807
display id: BBa_I732807
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ARL2A0103 (RBS+, TERM-)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:59Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_I732107
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_I732107
sequence: https://synbiohub.org/public/igem/BBa_I732807_sequence/1

BBa_I732107
type: Component
persistent identity: component1946184
display id: component1946184
version: 1
top level: BBa_I732807
access: public
definition: https://synbiohub.org/public/igem/BBa_I732107/1

BBa_B0034
type: Component
persistent identity: component1946183
display id: component1946183
version: 1
top level: BBa_I732807
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1946183
display id: annotation1946183
version: 1
top level: BBa_I732807
location: https://synbiohub.org/public/igem/BBa_I732807/annotation1946183/range1946183/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I732807/annotation1946183/range1946183/1
type: Range
persistent identity: range1946183
display id: range1946183
version: 1
direction: direction/reverse
top level: BBa_I732807
start: 1
end: 12
orientation: inline

BBa_I732107
type: SequenceAnnotation
persistent identity: annotation1946184
display id: annotation1946184
version: 1
top level: BBa_I732807
location: https://synbiohub.org/public/igem/BBa_I732807/annotation1946184/range1946184/1
component: BBa_I732107

https://synbiohub.org/public/igem/BBa_I732807/annotation1946184/range1946184/1
type: Range
persistent identity: range1946184
display id: range1946184
version: 1
direction: direction/reverse
top level: BBa_I732807
start: 19
end: 1104
orientation: inline

BBa_K775009
type: ComponentDefinition
persistent identity: BBa K775009
display id: BBa_K775009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Niacin Chimeric GPCR with Fus1-EGFP reporter (2)
creator: Sietske Grijseels
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:16Z
discontinued: false
dominant: false
group u list: _1027_
m user id: 0
owner id: 12035
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K775004
component: BBa_K775000
sequence annotation: BBa_K775000
sequence annotation: BBa_K775004
sequence: https://synbiohub.org/public/igem/BBa_K775009_sequence/1

BBa_K775004
type: Component
persistent identity: component2195575
display id: component2195575
version: 1
top level: BBa_K775009
access: public
definition: https://synbiohub.org/public/igem/BBa_K775004/1

BBa_K775000
type: Component
persistent identity: component2195587
display id: component2195587
version: 1
top level: BBa_K775009
access: public
definition: BBa_K775000

BBa_K775000
type: SequenceAnnotation
persistent identity: annotation2195587
display id: annotation2195587
version: 1
top level: BBa_K775009
location: https://synbiohub.org/public/igem/BBa_K775009/annotation2195587/range2195587/1
component: BBa_K775000

https://synbiohub.org/public/igem/BBa_K775009/annotation2195587/range2195587/1
type: Range
persistent identity: range2195587
display id: range2195587
version: 1
direction: direction/reverse
top level: BBa_K775009
start: 1175
end: 2588
orientation: inline

BBa_K775004
type: SequenceAnnotation
persistent identity: annotation2195575
display id: annotation2195575
version: 1
top level: BBa_K775009
location: https://synbiohub.org/public/igem/BBa_K775009/annotation2195575/range2195575/1
component: BBa_K775004

https://synbiohub.org/public/igem/BBa_K775009/annotation2195575/range2195575/1
type: Range
persistent identity: range2195575
display id: range2195575
version: 1
direction: direction/reverse
top level: BBa_K775009
start: 1
end: 1166
orientation: inline

BBa_I6106
type: ComponentDefinition
persistent identity: BBa I6106
display id: BBa_I6106
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter O_H Test R0078.E0430
creator: cconboy
created: 2004-05-09T11:00:00Z
modified: 2015-08-31T04:07:44Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 60
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_B0012
component: BBa_B0034
component: BBa_R0078
component: BBa_E0030
component: BBa_B0010
sequence annotation: BBa_E0030
sequence annotation: BBa_B0034
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_R0078
sequence: https://synbiohub.org/public/igem/BBa_I6106_sequence/1

BBa_B0012
type: Component
persistent identity: component949907
display id: component949907
version: 1
top level: BBa_I6106
access: public
definition: BBa_B0012

BBa_B0034
type: Component
persistent identity: component949890
display id: component949890
version: 1
top level: BBa_I6106
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0078
type: Component
persistent identity: component949885
display id: component949885
version: 1
top level: BBa_I6106
access: public
definition: cinR

BBa_E0030
type: Component
persistent identity: component949892
display id: component949892
version: 1
top level: BBa_I6106
access: public
definition: https://synbiohub.org/public/igem/BBa_E0030/1

BBa_B0010
type: Component
persistent identity: component949897
display id: component949897
version: 1
top level: BBa_I6106
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation949892
display id: annotation949892
version: 1
top level: BBa_I6106
location: https://synbiohub.org/public/igem/BBa_I6106/annotation949892/range949892/1
component: BBa_E0030

https://synbiohub.org/public/igem/BBa_I6106/annotation949892/range949892/1
type: Range
persistent identity: range949892
display id: range949892
version: 1
direction: direction/forward
top level: BBa_I6106
start: 252
end: 974
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation949890
display id: annotation949890
version: 1
top level: BBa_I6106
location: https://synbiohub.org/public/igem/BBa_I6106/annotation949890/range949890/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I6106/annotation949890/range949890/1
type: Range
persistent identity: range949890
display id: range949890
version: 1
direction: direction/forward
top level: BBa_I6106
start: 234
end: 245
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation949907
display id: annotation949907
version: 1
top level: BBa_I6106
location: https://synbiohub.org/public/igem/BBa_I6106/annotation949907/range949907/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I6106/annotation949907/range949907/1
type: Range
persistent identity: range949907
display id: range949907
version: 1
direction: direction/forward
top level: BBa_I6106
start: 1071
end: 1111
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation949897
display id: annotation949897
version: 1
top level: BBa_I6106
location: https://synbiohub.org/public/igem/BBa_I6106/annotation949897/range949897/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I6106/annotation949897/range949897/1
type: Range
persistent identity: range949897
display id: range949897
version: 1
direction: direction/forward
top level: BBa_I6106
start: 983
end: 1062
orientation: inline

BBa_R0078
type: SequenceAnnotation
persistent identity: annotation949885
display id: annotation949885
version: 1
top level: BBa_I6106
location: https://synbiohub.org/public/igem/BBa_I6106/annotation949885/range949885/1
component: BBa_R0078

https://synbiohub.org/public/igem/BBa_I6106/annotation949885/range949885/1
type: Range
persistent identity: range949885
display id: range949885
version: 1
direction: direction/forward
top level: BBa_I6106
start: 1
end: 225
orientation: inline

BBa_K876002
type: ComponentDefinition
persistent identity: BBa K876002
display id: BBa_K876002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pTet-LacI
creator: Alec Spinhirne
created: 2012-09-29T11:00:00Z
modified: 2015-05-08T01:13:38Z
discontinued: false
dominant: false
group u list: _1138_
m user id: 0
owner id: 13834
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Constitutive promoter for LacI, repressed by TetR
mutable notes: Biobrick placement
mutable provenance: Generated from biobricks
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_C0012
component: BBa_R0040
sequence annotation: BBa_R0040
sequence annotation: BBa_C0012
sequence: https://synbiohub.org/public/igem/BBa_K876002_sequence/1

BBa_C0012
type: Component
persistent identity: component2245093
display id: component2245093
version: 1
top level: BBa_K876002
access: public
definition: lacI

BBa_R0040
type: Component
persistent identity: component2245087
display id: component2245087
version: 1
top level: BBa_K876002
access: public
definition: p(tetR)

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2245087
display id: annotation2245087
version: 1
top level: BBa_K876002
location: https://synbiohub.org/public/igem/BBa_K876002/annotation2245087/range2245087/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K876002/annotation2245087/range2245087/1
type: Range
persistent identity: range2245087
display id: range2245087
version: 1
direction: direction/forward
top level: BBa_K876002
start: 1
end: 54
orientation: inline

BBa_C0012
type: SequenceAnnotation
persistent identity: annotation2245093
display id: annotation2245093
version: 1
top level: BBa_K876002
location: https://synbiohub.org/public/igem/BBa_K876002/annotation2245093/range2245093/1
component: BBa_C0012

https://synbiohub.org/public/igem/BBa_K876002/annotation2245093/range2245093/1
type: Range
persistent identity: range2245093
display id: range2245093
version: 1
direction: direction/forward
top level: BBa_K876002
start: 61
end: 1188
orientation: inline

BBa_K649303
type: ComponentDefinition
persistent identity: BBa K649303
display id: BBa_K649303
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PlacIQ-RBS-ispS
creator: Goshi Sugano
created: 2011-09-18T11:00:00Z
modified: 2015-05-08T01:13:00Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _826_
m user id: 0
owner id: 9613
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: RBS+ispS is same BBa_K649400. PlacIQ was used the part BBa_.
mutable notes: None.
mutable provenance: RBS+ispS : GeneArt Product PlacIQ : Part(BBa_)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_I14032
component: BBa_K649400
sequence annotation: BBa_K649400
sequence annotation: BBa_I14032
sequence: https://synbiohub.org/public/igem/BBa_K649303_sequence/1

BBa_I14032
type: Component
persistent identity: component2146397
display id: component2146397
version: 1
top level: BBa_K649303
access: public
definition: https://synbiohub.org/public/igem/BBa_I14032/1

BBa_K649400
type: Component
persistent identity: component2146399
display id: component2146399
version: 1
top level: BBa_K649303
access: public
definition: https://synbiohub.org/public/igem/BBa_K649400/1

BBa_K649400
type: SequenceAnnotation
persistent identity: annotation2146399
display id: annotation2146399
version: 1
top level: BBa_K649303
location: https://synbiohub.org/public/igem/BBa_K649303/annotation2146399/range2146399/1
component: BBa_K649400

https://synbiohub.org/public/igem/BBa_K649303/annotation2146399/range2146399/1
type: Range
persistent identity: range2146399
display id: range2146399
version: 1
direction: direction/reverse
top level: BBa_K649303
start: 46
end: 1908
orientation: inline

BBa_I14032
type: SequenceAnnotation
persistent identity: annotation2146397
display id: annotation2146397
version: 1
top level: BBa_K649303
location: https://synbiohub.org/public/igem/BBa_K649303/annotation2146397/range2146397/1
component: BBa_I14032

https://synbiohub.org/public/igem/BBa_K649303/annotation2146397/range2146397/1
type: Range
persistent identity: range2146397
display id: range2146397
version: 1
direction: direction/reverse
top level: BBa_K649303
start: 1
end: 37
orientation: inline

BBa_K741002
type: ComponentDefinition
persistent identity: BBa K741002
display id: BBa_K741002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: plac-RBS-GFP-T
creator: Wuyang Chen
created: 2012-07-23T11:00:00Z
modified: 2015-05-08T01:13:09Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _990_
m user id: 0
owner id: 11656
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Different strength of green fluorescence will be generated determined by the concentration of IPTG.
mutable notes: TBD
mutable provenance: BBa_R0010 BBa_E0040
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_R0010
component: BBa_I13504
sequence annotation: BBa_R0010
sequence annotation: BBa_I13504
sequence: https://synbiohub.org/public/igem/BBa_K741002_sequence/1

BBa_R0010
type: Component
persistent identity: component2177878
display id: component2177878
version: 1
top level: BBa_K741002
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_I13504
type: Component
persistent identity: component2177894
display id: component2177894
version: 1
top level: BBa_K741002
access: public
definition: BBa_I13504

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2177878
display id: annotation2177878
version: 1
top level: BBa_K741002
location: https://synbiohub.org/public/igem/BBa_K741002/annotation2177878/range2177878/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K741002/annotation2177878/range2177878/1
type: Range
persistent identity: range2177878
display id: range2177878
version: 1
direction: direction/forward
top level: BBa_K741002
start: 1
end: 200
orientation: inline

BBa_I13504
type: SequenceAnnotation
persistent identity: annotation2177894
display id: annotation2177894
version: 1
top level: BBa_K741002
location: https://synbiohub.org/public/igem/BBa_K741002/annotation2177894/range2177894/1
component: BBa_I13504

https://synbiohub.org/public/igem/BBa_K741002/annotation2177894/range2177894/1
type: Range
persistent identity: range2177894
display id: range2177894
version: 1
direction: direction/reverse
top level: BBa_K741002
start: 209
end: 1083
orientation: inline

BBa_K952003
type: ComponentDefinition
persistent identity: BBa K952003
display id: BBa_K952003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0034-YF1-B0034-FixJ-PfixK2-amilGFP
creator: Saimon Sharif
created: 2012-09-30T11:00:00Z
modified: 2015-05-08T01:13:49Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1220_
m user id: 0
owner id: 13332
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part is a composite of BBa_K592016, BBa_K592006, and BBa_K592010 in that order. When the cells are exposed to blue-light, they will express amilGFP, a yellow chromoprotein. This protein causes the cells to have a strong yellow color.
mutable notes: N/A
mutable provenance: BBa_K592016, BBa_K592006, and BBa_K592010 are available in the parts registry. They were obtained directly from the Uppsala-Sweden 2011 team.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_K592016
component: BBa_K592010
component: BBa_K592006
sequence annotation: BBa_K592016
sequence annotation: BBa_K592006
sequence annotation: BBa_K592010
sequence: https://synbiohub.org/public/igem/BBa_K952003_sequence/1

BBa_K592016
type: Component
persistent identity: component2205327
display id: component2205327
version: 1
top level: BBa_K952003
access: public
definition: https://synbiohub.org/public/igem/BBa_K592016/1

BBa_K592010
type: Component
persistent identity: component2205331
display id: component2205331
version: 1
top level: BBa_K952003
access: public
definition: https://synbiohub.org/public/igem/BBa_K592010/1

BBa_K592006
type: Component
persistent identity: component2205329
display id: component2205329
version: 1
top level: BBa_K952003
access: public
definition: https://synbiohub.org/public/igem/BBa_K592006/1

BBa_K592016
type: SequenceAnnotation
persistent identity: annotation2205327
display id: annotation2205327
version: 1
top level: BBa_K952003
location: https://synbiohub.org/public/igem/BBa_K952003/annotation2205327/range2205327/1
component: BBa_K592016

https://synbiohub.org/public/igem/BBa_K952003/annotation2205327/range2205327/1
type: Range
persistent identity: range2205327
display id: range2205327
version: 1
direction: direction/reverse
top level: BBa_K952003
start: 1
end: 1796
orientation: inline

BBa_K592006
type: SequenceAnnotation
persistent identity: annotation2205329
display id: annotation2205329
version: 1
top level: BBa_K952003
location: https://synbiohub.org/public/igem/BBa_K952003/annotation2205329/range2205329/1
component: BBa_K592006

https://synbiohub.org/public/igem/BBa_K952003/annotation2205329/range2205329/1
type: Range
persistent identity: range2205329
display id: range2205329
version: 1
direction: direction/reverse
top level: BBa_K952003
start: 1805
end: 2054
orientation: inline

BBa_K592010
type: SequenceAnnotation
persistent identity: annotation2205331
display id: annotation2205331
version: 1
top level: BBa_K952003
location: https://synbiohub.org/public/igem/BBa_K952003/annotation2205331/range2205331/1
component: BBa_K592010

https://synbiohub.org/public/igem/BBa_K952003/annotation2205331/range2205331/1
type: Range
persistent identity: range2205331
display id: range2205331
version: 1
direction: direction/reverse
top level: BBa_K952003
start: 2061
end: 2759
orientation: inline

BBa_K876011
type: ComponentDefinition
persistent identity: BBa K876011
display id: BBa_K876011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pTet-LasR-DT-pLas-LacI-RFP-ST-pLac-LuxI
creator: Timothy Cogan
created: 2012-09-29T11:00:00Z
modified: 2015-05-08T01:13:38Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _1138_
m user id: 0
owner id: 13836
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Population 2 for use with the dual strain toggle
mutable notes: Biobrick placement
mutable provenance: Generated from biobricks
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K081014
component: BBa_R0010
component: BBa_R0040
component: BBa_C0061
component: BBa_C0012
component: BBa_C0079
component: BBa_B0015
component: BBa_R0079
sequence annotation: BBa_C0061
sequence annotation: BBa_R0010
sequence annotation: BBa_R0040
sequence annotation: BBa_B0015
sequence annotation: BBa_R0079
sequence annotation: BBa_K081014
sequence annotation: BBa_C0079
sequence annotation: BBa_C0012
sequence: https://synbiohub.org/public/igem/BBa_K876011_sequence/1

BBa_K081014
type: Component
persistent identity: component2295865
display id: component2295865
version: 1
top level: BBa_K876011
access: public
definition: https://synbiohub.org/public/igem/BBa_K081014/1

BBa_R0010
type: Component
persistent identity: component2295866
display id: component2295866
version: 1
top level: BBa_K876011
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_R0040
type: Component
persistent identity: component2295828
display id: component2295828
version: 1
top level: BBa_K876011
access: public
definition: p(tetR)

BBa_C0061
type: Component
persistent identity: component2295874
display id: component2295874
version: 1
top level: BBa_K876011
access: public
definition: luxI

BBa_C0012
type: Component
persistent identity: component2295851
display id: component2295851
version: 1
top level: BBa_K876011
access: public
definition: lacI

BBa_C0079
type: Component
persistent identity: component2295837
display id: component2295837
version: 1
top level: BBa_K876011
access: public
definition: https://synbiohub.org/public/igem/BBa_C0079/1

BBa_B0015
type: Component
persistent identity: component2295844
display id: component2295844
version: 1
top level: BBa_K876011
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_R0079
type: Component
persistent identity: component2295849
display id: component2295849
version: 1
top level: BBa_K876011
access: public
definition: LasR+PAI

BBa_C0061
type: SequenceAnnotation
persistent identity: annotation2295874
display id: annotation2295874
version: 1
top level: BBa_K876011
location: https://synbiohub.org/public/igem/BBa_K876011/annotation2295874/range2295874/1
component: BBa_C0061

https://synbiohub.org/public/igem/BBa_K876011/annotation2295874/range2295874/1
type: Range
persistent identity: range2295874
display id: range2295874
version: 1
direction: direction/forward
top level: BBa_K876011
start: 3299
end: 3916
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2295866
display id: annotation2295866
version: 1
top level: BBa_K876011
location: https://synbiohub.org/public/igem/BBa_K876011/annotation2295866/range2295866/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K876011/annotation2295866/range2295866/1
type: Range
persistent identity: range2295866
display id: range2295866
version: 1
direction: direction/forward
top level: BBa_K876011
start: 3093
end: 3292
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2295828
display id: annotation2295828
version: 1
top level: BBa_K876011
location: https://synbiohub.org/public/igem/BBa_K876011/annotation2295828/range2295828/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K876011/annotation2295828/range2295828/1
type: Range
persistent identity: range2295828
display id: range2295828
version: 1
direction: direction/forward
top level: BBa_K876011
start: 1
end: 54
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2295844
display id: annotation2295844
version: 1
top level: BBa_K876011
location: https://synbiohub.org/public/igem/BBa_K876011/annotation2295844/range2295844/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K876011/annotation2295844/range2295844/1
type: Range
persistent identity: range2295844
display id: range2295844
version: 1
direction: direction/reverse
top level: BBa_K876011
start: 850
end: 978
orientation: inline

BBa_R0079
type: SequenceAnnotation
persistent identity: annotation2295849
display id: annotation2295849
version: 1
top level: BBa_K876011
location: https://synbiohub.org/public/igem/BBa_K876011/annotation2295849/range2295849/1
component: BBa_R0079

https://synbiohub.org/public/igem/BBa_K876011/annotation2295849/range2295849/1
type: Range
persistent identity: range2295849
display id: range2295849
version: 1
direction: direction/reverse
top level: BBa_K876011
start: 987
end: 1143
orientation: inline

BBa_K081014
type: SequenceAnnotation
persistent identity: annotation2295865
display id: annotation2295865
version: 1
top level: BBa_K876011
location: https://synbiohub.org/public/igem/BBa_K876011/annotation2295865/range2295865/1
component: BBa_K081014

https://synbiohub.org/public/igem/BBa_K876011/annotation2295865/range2295865/1
type: Range
persistent identity: range2295865
display id: range2295865
version: 1
direction: direction/reverse
top level: BBa_K876011
start: 2311
end: 3084
orientation: inline

BBa_C0079
type: SequenceAnnotation
persistent identity: annotation2295837
display id: annotation2295837
version: 1
top level: BBa_K876011
location: https://synbiohub.org/public/igem/BBa_K876011/annotation2295837/range2295837/1
component: BBa_C0079

https://synbiohub.org/public/igem/BBa_K876011/annotation2295837/range2295837/1
type: Range
persistent identity: range2295837
display id: range2295837
version: 1
direction: direction/reverse
top level: BBa_K876011
start: 61
end: 841
orientation: inline

BBa_C0012
type: SequenceAnnotation
persistent identity: annotation2295851
display id: annotation2295851
version: 1
top level: BBa_K876011
location: https://synbiohub.org/public/igem/BBa_K876011/annotation2295851/range2295851/1
component: BBa_C0012

https://synbiohub.org/public/igem/BBa_K876011/annotation2295851/range2295851/1
type: Range
persistent identity: range2295851
display id: range2295851
version: 1
direction: direction/forward
top level: BBa_K876011
start: 1150
end: 2277
orientation: inline

CMV AID pA
type: ComponentDefinition
persistent identity: BBa K929000
display id: BBa_K929000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AID with CMV promoter and hGH-polyadenylation signal sequence
creator: Potsdam Bioware 2012 iGEM
created: 2012-09-15T11:00:00Z
modified: 2015-05-08T01:13:46Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1194_
m user id: 0
owner id: 14457
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: The BioBrick wtAID is an extended version of the existing AID BioBrick (BBa_K103001). It is built of 3 parts: CMV promoter, AID and hGH-polyA tail. AID: AID is known to be responsible for somatic hypermutation and the class-switch recombination of immunoglobulin in B cells. This enzyme of 28 kDa originally occurs in B cells but does also show activity after transfection into CHO cells. AID induces the deamination of cytidine to uridine at actively transcribed single strand DNA. The replacement of cytidine by uridine leads to a mismatch during DNA replication and integrates a single base substitution predominantly in the immunoglobulin genes. CMV promoter: CMV stands for cytomegalovirus. The CMV promoter is commonly used due to its very strong activity, and effectivity in a broad range of cell types. The BioBrick is therefore improved via addition of the strong promoter. hGH-polyA tail: The polyA tail consists of multiple adenosine monophosphates. hGH-polyA tail is a significant part for the translation and stability of mRNA. In eukaryotes, polyadenylation is part of the process that produces mature messenger RNA (mRNA) for translation. It, therefore, forms part of the larger process of gene expression.
mutable notes: bla bla
mutable provenance: It is a composite part consisting of 3 other Biobricks.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: UGA
sequence annotation: BBa_K404108
sequence annotation: BBa_I712004
sequence annotation: AUG
sequence annotation: BBa_K103001
sequence annotation: CMV promoter
sequence annotation: hGH terminator
sequence annotation: NcoI
sequence annotation: AID
sequence: https://synbiohub.org/public/igem/BBa_K929000_sequence/1

UGA
type: SequenceAnnotation
persistent identity: annotation2197701
display id: annotation2197701
version: 1
top level: CMV AID pA
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K929000/annotation2197701/range2197701/1

https://synbiohub.org/public/igem/BBa_K929000/annotation2197701/range2197701/1
type: Range
persistent identity: range2197701
display id: range2197701
version: 1
direction: direction/forward
top level: CMV AID pA
start: 1259
end: 1262
orientation: inline

BBa_K404108
type: SequenceAnnotation
persistent identity: annotation2197703
display id: annotation2197703
version: 1
top level: CMV AID pA
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K929000/annotation2197703/range2197703/1

https://synbiohub.org/public/igem/BBa_K929000/annotation2197703/range2197703/1
type: Range
persistent identity: range2197703
display id: range2197703
version: 1
direction: direction/reverse
top level: CMV AID pA
start: 1276
end: 1754
orientation: inline

BBa_I712004
type: SequenceAnnotation
persistent identity: annotation2197696
display id: annotation2197696
version: 1
top level: CMV AID pA
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K929000/annotation2197696/range2197696/1

https://synbiohub.org/public/igem/BBa_K929000/annotation2197696/range2197696/1
type: Range
persistent identity: range2197696
display id: range2197696
version: 1
direction: direction/reverse
top level: CMV AID pA
start: 1
end: 654
orientation: inline

AUG
type: SequenceAnnotation
persistent identity: annotation2197700
display id: annotation2197700
version: 1
top level: CMV AID pA
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K929000/annotation2197700/range2197700/1

https://synbiohub.org/public/igem/BBa_K929000/annotation2197700/range2197700/1
type: Range
persistent identity: range2197700
display id: range2197700
version: 1
direction: direction/forward
top level: CMV AID pA
start: 665
end: 667
orientation: inline

BBa_K103001
type: SequenceAnnotation
persistent identity: annotation2197698
display id: annotation2197698
version: 1
top level: CMV AID pA
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K929000/annotation2197698/range2197698/1

https://synbiohub.org/public/igem/BBa_K929000/annotation2197698/range2197698/1
type: Range
persistent identity: range2197698
display id: range2197698
version: 1
direction: direction/reverse
top level: CMV AID pA
start: 663
end: 1262
orientation: inline

CMV promoter
type: SequenceAnnotation
persistent identity: annotation2197695
display id: annotation2197695
version: 1
top level: CMV AID pA
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K929000/annotation2197695/range2197695/1

https://synbiohub.org/public/igem/BBa_K929000/annotation2197695/range2197695/1
type: Range
persistent identity: range2197695
display id: range2197695
version: 1
direction: direction/forward
top level: CMV AID pA
start: 1
end: 654
orientation: inline

hGH terminator
type: SequenceAnnotation
persistent identity: annotation2197702
display id: annotation2197702
version: 1
top level: CMV AID pA
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_K929000/annotation2197702/range2197702/1

https://synbiohub.org/public/igem/BBa_K929000/annotation2197702/range2197702/1
type: Range
persistent identity: range2197702
display id: range2197702
version: 1
direction: direction/reverse
top level: CMV AID pA
start: 1276
end: 1754
orientation: inline

NcoI
type: SequenceAnnotation
persistent identity: annotation2197697
display id: annotation2197697
version: 1
top level: CMV AID pA
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K929000/annotation2197697/range2197697/1

https://synbiohub.org/public/igem/BBa_K929000/annotation2197697/range2197697/1
type: Range
persistent identity: range2197697
display id: range2197697
version: 1
direction: direction/forward
top level: CMV AID pA
start: 663
end: 668
orientation: inline

AID
type: SequenceAnnotation
persistent identity: annotation2197699
display id: annotation2197699
version: 1
top level: CMV AID pA
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K929000/annotation2197699/range2197699/1

https://synbiohub.org/public/igem/BBa_K929000/annotation2197699/range2197699/1
type: Range
persistent identity: range2197699
display id: range2197699
version: 1
direction: direction/forward
top level: CMV AID pA
start: 665
end: 1262
orientation: inline

BBa_K953002
type: ComponentDefinition
persistent identity: BBa K953002
display id: BBa_K953002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Agrobacterium tumefaciens Bacteriophytochrome with RBS (E. coli Codon Optimised)
creator: Daniel Winter
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1221_
m user id: 0
owner id: 14275
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Agrobacterium tumefaciens bacteriophytochrome, codon optimised for expression in E. coli. In the presence of biliverdin the bacteriophytochrome will undergo a conformation change. This complex can then absorb red light(620-750 nm) to excite the complex and result in a phenotypic change from blue to green. This can be reversed by far-red light (700-800 nm) or will revert from green to blue over time. As E. coli does not produce biliverdin, heme oxygenase (Part BBa_K953000) can be coupled with this part to activate the oxidation of heme to produce biliverdin or by the addition of biliverdin to the cells.
mutable notes: Optimised for expression in E. coli
mutable provenance: Agrobacterium tumefaciens
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: Start Codon
sequence annotation: BBa_K953002
sequence annotation: RBS
sequence annotation: Stop
sequence annotation: Bacteriophytochrome
sequence: https://synbiohub.org/public/igem/BBa_K953002_sequence/1

Start Codon
type: SequenceAnnotation
persistent identity: annotation2196369
display id: annotation2196369
version: 1
top level: BBa_K953002
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K953002/annotation2196369/range2196369/1

https://synbiohub.org/public/igem/BBa_K953002/annotation2196369/range2196369/1
type: Range
persistent identity: range2196369
display id: range2196369
version: 1
direction: direction/forward
top level: BBa_K953002
start: 23
end: 25
orientation: inline

BBa_K953002
type: SequenceAnnotation
persistent identity: annotation2196372
display id: annotation2196372
version: 1
top level: BBa_K953002
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K953002/annotation2196372/range2196372/1

https://synbiohub.org/public/igem/BBa_K953002/annotation2196372/range2196372/1
type: Range
persistent identity: range2196372
display id: range2196372
version: 1
direction: direction/reverse
top level: BBa_K953002
start: 1
end: 2236
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2196368
display id: annotation2196368
version: 1
top level: BBa_K953002
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K953002/annotation2196368/range2196368/1

https://synbiohub.org/public/igem/BBa_K953002/annotation2196368/range2196368/1
type: Range
persistent identity: range2196368
display id: range2196368
version: 1
direction: direction/forward
top level: BBa_K953002
start: 1
end: 22
orientation: inline

Stop
type: SequenceAnnotation
persistent identity: annotation2196371
display id: annotation2196371
version: 1
top level: BBa_K953002
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K953002/annotation2196371/range2196371/1

https://synbiohub.org/public/igem/BBa_K953002/annotation2196371/range2196371/1
type: Range
persistent identity: range2196371
display id: range2196371
version: 1
direction: direction/forward
top level: BBa_K953002
start: 2231
end: 2236
orientation: inline

Bacteriophytochrome
type: SequenceAnnotation
persistent identity: annotation2196370
display id: annotation2196370
version: 1
top level: BBa_K953002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K953002/annotation2196370/range2196370/1

https://synbiohub.org/public/igem/BBa_K953002/annotation2196370/range2196370/1
type: Range
persistent identity: range2196370
display id: range2196370
version: 1
direction: direction/forward
top level: BBa_K953002
start: 26
end: 2230
orientation: inline

luxI
type: ComponentDefinition
persistent identity: BBa C0061
display id: BBa_C0061
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: autoinducer synthetase for AHL
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Synthesizes 3OC6HSL, which binds to LuxR. The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the Lux repressor, LuxR. Two molecules of LuxR protein form a complex with two molecules the signalling compound HSL. This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
mutable notes: An LVA tail (sequence: AANDENYALVA) was added to increase protein degradation. .
mutable provenance: V. fischeri AF170104
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: BBa_C0061
sequence annotation: Help:Barcodes
sequence annotation: luxI
sequence annotation: LVA
sequence: https://synbiohub.org/public/igem/BBa_C0061_sequence/1

BBa_C0061
type: SequenceAnnotation
persistent identity: annotation7038
display id: annotation7038
version: 1
top level: luxI
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_C0061/annotation7038/range7038/1

https://synbiohub.org/public/igem/BBa_C0061/annotation7038/range7038/1
type: Range
persistent identity: range7038
display id: range7038
version: 1
direction: direction/forward
top level: luxI
start: 1
end: 618
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213985
display id: annotation2213985
version: 1
top level: luxI
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_C0061/annotation2213985/range2213985/1

https://synbiohub.org/public/igem/BBa_C0061/annotation2213985/range2213985/1
type: Range
persistent identity: range2213985
display id: range2213985
version: 1
direction: direction/forward
top level: luxI
start: 619
end: 643
orientation: inline

luxI
type: SequenceAnnotation
persistent identity: annotation1761
display id: annotation1761
version: 1
top level: luxI
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0061/annotation1761/range1761/1

https://synbiohub.org/public/igem/BBa_C0061/annotation1761/range1761/1
type: Range
persistent identity: range1761
display id: range1761
version: 1
direction: direction/forward
top level: luxI
start: 1
end: 579
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation1760
display id: annotation1760
version: 1
top level: luxI
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_C0061/annotation1760/range1760/1

https://synbiohub.org/public/igem/BBa_C0061/annotation1760/range1760/1
type: Range
persistent identity: range1760
display id: range1760
version: 1
direction: direction/forward
top level: luxI
start: 580
end: 611
orientation: inline

BBa_I6032
type: ComponentDefinition
persistent identity: BBa I6032
display id: BBa_I6032
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter O_H Test R0040.E0430
creator: Caitlin Conboy and Jennifer Braff
created: 2004-03-16T12:00:00Z
modified: 2015-08-31T04:07:43Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_B0010
component: BBa_R0040
component: BBa_B0012
component: BBa_E0030
component: BBa_B0034
sequence annotation: BBa_B0012
sequence annotation: BBa_R0040
sequence annotation: BBa_B0010
sequence annotation: BBa_E0030
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_I6032_sequence/1

BBa_B0010
type: Component
persistent identity: component948362
display id: component948362
version: 1
top level: BBa_I6032
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_R0040
type: Component
persistent identity: component948347
display id: component948347
version: 1
top level: BBa_I6032
access: public
definition: p(tetR)

BBa_B0012
type: Component
persistent identity: component948372
display id: component948372
version: 1
top level: BBa_I6032
access: public
definition: BBa_B0012

BBa_E0030
type: Component
persistent identity: component948357
display id: component948357
version: 1
top level: BBa_I6032
access: public
definition: https://synbiohub.org/public/igem/BBa_E0030/1

BBa_B0034
type: Component
persistent identity: component948355
display id: component948355
version: 1
top level: BBa_I6032
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation948372
display id: annotation948372
version: 1
top level: BBa_I6032
location: https://synbiohub.org/public/igem/BBa_I6032/annotation948372/range948372/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I6032/annotation948372/range948372/1
type: Range
persistent identity: range948372
display id: range948372
version: 1
direction: direction/forward
top level: BBa_I6032
start: 900
end: 940
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation948347
display id: annotation948347
version: 1
top level: BBa_I6032
location: https://synbiohub.org/public/igem/BBa_I6032/annotation948347/range948347/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_I6032/annotation948347/range948347/1
type: Range
persistent identity: range948347
display id: range948347
version: 1
direction: direction/forward
top level: BBa_I6032
start: 1
end: 54
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation948362
display id: annotation948362
version: 1
top level: BBa_I6032
location: https://synbiohub.org/public/igem/BBa_I6032/annotation948362/range948362/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I6032/annotation948362/range948362/1
type: Range
persistent identity: range948362
display id: range948362
version: 1
direction: direction/forward
top level: BBa_I6032
start: 812
end: 891
orientation: inline

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation948357
display id: annotation948357
version: 1
top level: BBa_I6032
location: https://synbiohub.org/public/igem/BBa_I6032/annotation948357/range948357/1
component: BBa_E0030

https://synbiohub.org/public/igem/BBa_I6032/annotation948357/range948357/1
type: Range
persistent identity: range948357
display id: range948357
version: 1
direction: direction/forward
top level: BBa_I6032
start: 81
end: 803
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation948355
display id: annotation948355
version: 1
top level: BBa_I6032
location: https://synbiohub.org/public/igem/BBa_I6032/annotation948355/range948355/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I6032/annotation948355/range948355/1
type: Range
persistent identity: range948355
display id: range948355
version: 1
direction: direction/forward
top level: BBa_I6032
start: 63
end: 74
orientation: inline

BBa_K808014
type: ComponentDefinition
persistent identity: BBa K808014
display id: BBa_K808014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AroY: Catalyzes protocatechuate to catechol
creator: Sascha Hein, Sven Rumpf, Daniel Sachs, Arne Wehling, Philipp Rottmann
created: 2012-09-01T11:00:00Z
modified: 2015-05-08T01:13:26Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1065_
m user id: 0
owner id: 11792
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K808014_sequence/1

BBa_I13457
type: ComponentDefinition
persistent identity: BBa I13457
display id: BBa_I13457
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Improved insertion site with two RNAse E cut sites
creator: jkm
created: 2005-07-18T11:00:00Z
modified: 2015-08-31T04:07:34Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Shortened RNAse E site, insertion site, duplicate RNAse E site, and a trailing 5' hairpin (HP16 from Smolke et al., 2002).
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: RNAse E cut site (between the bases)
sequence annotation: RNAse E site
sequence annotation: Hairpin
sequence annotation: Insertion site
sequence: https://synbiohub.org/public/igem/BBa_I13457_sequence/1

RNAse E cut site (between the bases)
type: SequenceAnnotation
persistent identity: annotation1588511
display id: annotation1588511
version: 1
top level: BBa_I13457
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I13457/annotation1588511/range1588511/1

https://synbiohub.org/public/igem/BBa_I13457/annotation1588511/range1588511/1
type: Range
persistent identity: range1588511
display id: range1588511
version: 1
direction: direction/forward
top level: BBa_I13457
start: 9
end: 10
orientation: inline

RNAse E site
type: SequenceAnnotation
persistent identity: annotation1588510
display id: annotation1588510
version: 1
top level: BBa_I13457
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I13457/annotation1588510/range1588510/1

https://synbiohub.org/public/igem/BBa_I13457/annotation1588510/range1588510/1
type: Range
persistent identity: range1588510
display id: range1588510
version: 1
direction: direction/forward
top level: BBa_I13457
start: 1
end: 21
orientation: inline

RNAse E site
type: SequenceAnnotation
persistent identity: annotation1588513
display id: annotation1588513
version: 1
top level: BBa_I13457
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I13457/annotation1588513/range1588513/1

https://synbiohub.org/public/igem/BBa_I13457/annotation1588513/range1588513/1
type: Range
persistent identity: range1588513
display id: range1588513
version: 1
direction: direction/forward
top level: BBa_I13457
start: 56
end: 76
orientation: inline

Hairpin
type: SequenceAnnotation
persistent identity: annotation1588515
display id: annotation1588515
version: 1
top level: BBa_I13457
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_I13457/annotation1588515/range1588515/1

https://synbiohub.org/public/igem/BBa_I13457/annotation1588515/range1588515/1
type: Range
persistent identity: range1588515
display id: range1588515
version: 1
direction: direction/forward
top level: BBa_I13457
start: 77
end: 111
orientation: inline

Insertion site
type: SequenceAnnotation
persistent identity: annotation1588512
display id: annotation1588512
version: 1
top level: BBa_I13457
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I13457/annotation1588512/range1588512/1

https://synbiohub.org/public/igem/BBa_I13457/annotation1588512/range1588512/1
type: Range
persistent identity: range1588512
display id: range1588512
version: 1
direction: direction/forward
top level: BBa_I13457
start: 22
end: 55
orientation: inline

RNAse E cut site (between the bases)
type: SequenceAnnotation
persistent identity: annotation1588514
display id: annotation1588514
version: 1
top level: BBa_I13457
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I13457/annotation1588514/range1588514/1

https://synbiohub.org/public/igem/BBa_I13457/annotation1588514/range1588514/1
type: Range
persistent identity: range1588514
display id: range1588514
version: 1
direction: direction/forward
top level: BBa_I13457
start: 64
end: 65
orientation: inline

BBa_J202010
type: ComponentDefinition
persistent identity: BBa J202010
display id: BBa_J202010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PBAD Mutant#11
creator: Jiyeon Park
created: 2012-07-25T11:00:00Z
modified: 2015-08-31T04:08:37Z
discontinued: false
dominant: false
group u list: _910_
m user id: 0
owner id: 11164
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is PBAD (BBa_I13453) mutant. -58 relative to the araC transcriptional start site was changed from A to C.
mutable notes: In order to regulate transcription from PBAD, araC binds three different sites called araO2, araI1, and araI2. In the absence of L-arabinose, araC binds both araO2 and araI1 and bends DNA preventing transcription from PBAD. In the presence of L-arabinose, however, araC has a conformational change because of L-arabinose and binds araI1 and araI2, enabling transcription from PBAD. Like the wild-type (I13453), this part does not have araO2 site which is essential for araC repressor function. Therefore, araC acts as an activator in the presence of L-arabinose.
mutable provenance: This mutant was created by site-directed mutagenesis.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Mutation
sequence annotation: Transcription Start
sequence annotation: araI1
sequence annotation: araI2
sequence: https://synbiohub.org/public/igem/BBa_J202010_sequence/1

Mutation
type: SequenceAnnotation
persistent identity: annotation2178457
display id: annotation2178457
version: 1
top level: BBa_J202010
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_J202010/annotation2178457/range2178457/1

https://synbiohub.org/public/igem/BBa_J202010/annotation2178457/range2178457/1
type: Range
persistent identity: range2178457
display id: range2178457
version: 1
direction: direction/forward
top level: BBa_J202010
start: 54
end: 54
orientation: inline

Transcription Start
type: SequenceAnnotation
persistent identity: annotation2178458
display id: annotation2178458
version: 1
top level: BBa_J202010
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_J202010/annotation2178458/range2178458/1

https://synbiohub.org/public/igem/BBa_J202010/annotation2178458/range2178458/1
type: Range
persistent identity: range2178458
display id: range2178458
version: 1
direction: direction/forward
top level: BBa_J202010
start: 112
end: 112
orientation: inline

araI1
type: SequenceAnnotation
persistent identity: annotation2178459
display id: annotation2178459
version: 1
top level: BBa_J202010
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J202010/annotation2178459/range2178459/1

https://synbiohub.org/public/igem/BBa_J202010/annotation2178459/range2178459/1
type: Range
persistent identity: range2178459
display id: range2178459
version: 1
direction: direction/forward
top level: BBa_J202010
start: 40
end: 56
orientation: inline

araI2
type: SequenceAnnotation
persistent identity: annotation2178460
display id: annotation2178460
version: 1
top level: BBa_J202010
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J202010/annotation2178460/range2178460/1

https://synbiohub.org/public/igem/BBa_J202010/annotation2178460/range2178460/1
type: Range
persistent identity: range2178460
display id: range2178460
version: 1
direction: direction/forward
top level: BBa_J202010
start: 61
end: 77
orientation: inline

BBa_I6086
type: ComponentDefinition
persistent identity: BBa I6086
display id: BBa_I6086
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter O_H Test R1062.E0432
creator: Caitlin Conboy and Jennifer Braff
created: 2004-03-17T12:00:00Z
modified: 2015-08-31T04:07:43Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_B0012
component: BBa_B0034
component: BBa_R1062
component: BBa_E0032
component: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_E0032
sequence annotation: BBa_B0034
sequence annotation: BBa_B0010
sequence annotation: BBa_R1062
sequence: https://synbiohub.org/public/igem/BBa_I6086_sequence/1

BBa_B0012
type: Component
persistent identity: component949630
display id: component949630
version: 1
top level: BBa_I6086
access: public
definition: BBa_B0012

BBa_B0034
type: Component
persistent identity: component949600
display id: component949600
version: 1
top level: BBa_I6086
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R1062
type: Component
persistent identity: component949592
display id: component949592
version: 1
top level: BBa_I6086
access: public
definition: lux pR

BBa_E0032
type: Component
persistent identity: component949612
display id: component949612
version: 1
top level: BBa_I6086
access: public
definition: YFP

BBa_B0010
type: Component
persistent identity: component949620
display id: component949620
version: 1
top level: BBa_I6086
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation949630
display id: annotation949630
version: 1
top level: BBa_I6086
location: https://synbiohub.org/public/igem/BBa_I6086/annotation949630/range949630/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I6086/annotation949630/range949630/1
type: Range
persistent identity: range949630
display id: range949630
version: 1
direction: direction/forward
top level: BBa_I6086
start: 941
end: 981
orientation: inline

BBa_E0032
type: SequenceAnnotation
persistent identity: annotation949612
display id: annotation949612
version: 1
top level: BBa_I6086
location: https://synbiohub.org/public/igem/BBa_I6086/annotation949612/range949612/1
component: BBa_E0032

https://synbiohub.org/public/igem/BBa_I6086/annotation949612/range949612/1
type: Range
persistent identity: range949612
display id: range949612
version: 1
direction: direction/forward
top level: BBa_I6086
start: 83
end: 844
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation949600
display id: annotation949600
version: 1
top level: BBa_I6086
location: https://synbiohub.org/public/igem/BBa_I6086/annotation949600/range949600/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I6086/annotation949600/range949600/1
type: Range
persistent identity: range949600
display id: range949600
version: 1
direction: direction/forward
top level: BBa_I6086
start: 65
end: 76
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation949620
display id: annotation949620
version: 1
top level: BBa_I6086
location: https://synbiohub.org/public/igem/BBa_I6086/annotation949620/range949620/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I6086/annotation949620/range949620/1
type: Range
persistent identity: range949620
display id: range949620
version: 1
direction: direction/forward
top level: BBa_I6086
start: 853
end: 932
orientation: inline

BBa_R1062
type: SequenceAnnotation
persistent identity: annotation949592
display id: annotation949592
version: 1
top level: BBa_I6086
location: https://synbiohub.org/public/igem/BBa_I6086/annotation949592/range949592/1
component: BBa_R1062

https://synbiohub.org/public/igem/BBa_I6086/annotation949592/range949592/1
type: Range
persistent identity: range949592
display id: range949592
version: 1
direction: direction/forward
top level: BBa_I6086
start: 1
end: 56
orientation: inline

BBa_E0430
type: ComponentDefinition
persistent identity: BBa E0430
display id: BBa_E0430
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: EYFP (RBS+ LVA- TERM) (B0034.E0030.B0015)
creator: Caitlin Conboy
created: 2004-03-15T12:00:00Z
modified: 2015-08-31T04:07:26Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Standard YFP Output Device -LVA tag
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_E0030
component: BBa_B0012
component: BBa_B0010
component: BBa_B0034
sequence annotation: BBa_B0012
sequence annotation: BBa_E0030
sequence annotation: BBa_B0034
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_E0430_sequence/1

BBa_E0030
type: Component
persistent identity: component942887
display id: component942887
version: 1
top level: BBa_E0430
access: public
definition: https://synbiohub.org/public/igem/BBa_E0030/1

BBa_B0012
type: Component
persistent identity: component942902
display id: component942902
version: 1
top level: BBa_E0430
access: public
definition: BBa_B0012

BBa_B0010
type: Component
persistent identity: component942892
display id: component942892
version: 1
top level: BBa_E0430
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component942885
display id: component942885
version: 1
top level: BBa_E0430
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation942902
display id: annotation942902
version: 1
top level: BBa_E0430
location: https://synbiohub.org/public/igem/BBa_E0430/annotation942902/range942902/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_E0430/annotation942902/range942902/1
type: Range
persistent identity: range942902
display id: range942902
version: 1
direction: direction/forward
top level: BBa_E0430
start: 838
end: 878
orientation: inline

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation942887
display id: annotation942887
version: 1
top level: BBa_E0430
location: https://synbiohub.org/public/igem/BBa_E0430/annotation942887/range942887/1
component: BBa_E0030

https://synbiohub.org/public/igem/BBa_E0430/annotation942887/range942887/1
type: Range
persistent identity: range942887
display id: range942887
version: 1
direction: direction/forward
top level: BBa_E0430
start: 19
end: 741
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation942885
display id: annotation942885
version: 1
top level: BBa_E0430
location: https://synbiohub.org/public/igem/BBa_E0430/annotation942885/range942885/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_E0430/annotation942885/range942885/1
type: Range
persistent identity: range942885
display id: range942885
version: 1
direction: direction/forward
top level: BBa_E0430
start: 1
end: 12
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation942892
display id: annotation942892
version: 1
top level: BBa_E0430
location: https://synbiohub.org/public/igem/BBa_E0430/annotation942892/range942892/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_E0430/annotation942892/range942892/1
type: Range
persistent identity: range942892
display id: range942892
version: 1
direction: direction/forward
top level: BBa_E0430
start: 750
end: 829
orientation: inline

BBa_S01022
type: ComponentDefinition
persistent identity: BBa S01022
display id: BBa_S01022
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0034.E0020
creator: Randy Rettberg
created: 2005-06-15T11:00:00Z
modified: 2015-05-08T01:14:17Z
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 25
owning group id: 1
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Intermediate Part from Box 7
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_E0020
component: BBa_B0034
sequence annotation: BBa_E0020
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_S01022_sequence/1

BBa_E0020
type: Component
persistent identity: component1520219
display id: component1520219
version: 1
top level: BBa_S01022
access: public
definition: ecfp

BBa_B0034
type: Component
persistent identity: component1520217
display id: component1520217
version: 1
top level: BBa_S01022
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_E0020
type: SequenceAnnotation
persistent identity: annotation1520219
display id: annotation1520219
version: 1
top level: BBa_S01022
location: https://synbiohub.org/public/igem/BBa_S01022/annotation1520219/range1520219/1
component: BBa_E0020

https://synbiohub.org/public/igem/BBa_S01022/annotation1520219/range1520219/1
type: Range
persistent identity: range1520219
display id: range1520219
version: 1
direction: direction/forward
top level: BBa_S01022
start: 19
end: 741
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1520217
display id: annotation1520217
version: 1
top level: BBa_S01022
location: https://synbiohub.org/public/igem/BBa_S01022/annotation1520217/range1520217/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_S01022/annotation1520217/range1520217/1
type: Range
persistent identity: range1520217
display id: range1520217
version: 1
direction: direction/forward
top level: BBa_S01022
start: 1
end: 12
orientation: inline

BBa_P0440
type: ComponentDefinition
persistent identity: BBa P0440
display id: BBa_P0440
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PoPS -> TetR [S0151]
creator: Randy Rettberg
created: 2004-04-26T11:00:00Z
modified: 2015-05-08T01:14:10Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_C0040
component: BBa_B0012
component: BBa_B0010
component: BBa_B0034
sequence annotation: BBa_B0010
sequence annotation: BBa_C0040
sequence annotation: BBa_B0034
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_P0440_sequence/1

BBa_C0040
type: Component
persistent identity: component944896
display id: component944896
version: 1
top level: BBa_P0440
access: public
definition: tetR

BBa_B0012
type: Component
persistent identity: component944912
display id: component944912
version: 1
top level: BBa_P0440
access: public
definition: BBa_B0012

BBa_B0010
type: Component
persistent identity: component944902
display id: component944902
version: 1
top level: BBa_P0440
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component944886
display id: component944886
version: 1
top level: BBa_P0440
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation944902
display id: annotation944902
version: 1
top level: BBa_P0440
location: https://synbiohub.org/public/igem/BBa_P0440/annotation944902/range944902/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_P0440/annotation944902/range944902/1
type: Range
persistent identity: range944902
display id: range944902
version: 1
direction: direction/forward
top level: BBa_P0440
start: 712
end: 791
orientation: inline

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation944896
display id: annotation944896
version: 1
top level: BBa_P0440
location: https://synbiohub.org/public/igem/BBa_P0440/annotation944896/range944896/1
component: BBa_C0040

https://synbiohub.org/public/igem/BBa_P0440/annotation944896/range944896/1
type: Range
persistent identity: range944896
display id: range944896
version: 1
direction: direction/forward
top level: BBa_P0440
start: 19
end: 678
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation944886
display id: annotation944886
version: 1
top level: BBa_P0440
location: https://synbiohub.org/public/igem/BBa_P0440/annotation944886/range944886/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_P0440/annotation944886/range944886/1
type: Range
persistent identity: range944886
display id: range944886
version: 1
direction: direction/forward
top level: BBa_P0440
start: 1
end: 12
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation944912
display id: annotation944912
version: 1
top level: BBa_P0440
location: https://synbiohub.org/public/igem/BBa_P0440/annotation944912/range944912/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_P0440/annotation944912/range944912/1
type: Range
persistent identity: range944912
display id: range944912
version: 1
direction: direction/forward
top level: BBa_P0440
start: 800
end: 840
orientation: inline

BBa_K812020
type: ComponentDefinition
persistent identity: BBa K812020
display id: BBa_K812020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Eukaryotic PoPs -> IaaH. Indoleacetamide hydrolyase for Auxin generator, with kozak
creator: William Rostain
created: 2012-09-19T11:00:00Z
modified: 2015-05-08T01:13:27Z
discontinued: false
dominant: true
group u list: _1069_
m user id: 0
owner id: 7053
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part contains the coding sequence for Indoleacetamide hydrolyase preceded by a Kozak sequence, for expression in eukaryotic plasmids.
mutable notes: Kozak is included, as it cannot be added by biobrick. This part can be used to assemble biobricks for integration onto Xenopus tropicalis genome. It can also be used directly by injection into a fertilised egg, using the ready to use Xenopus plasmid BBa_K812000.
mutable provenance: This part was created from BBa_K515100, itself from P. savastanoi (By Imperial college)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Kozak sequence
sequence annotation: IaaH
sequence: https://synbiohub.org/public/igem/BBa_K812020_sequence/1

Kozak sequence
type: SequenceAnnotation
persistent identity: annotation2187775
display id: annotation2187775
version: 1
top level: BBa_K812020
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K812020/annotation2187775/range2187775/1

https://synbiohub.org/public/igem/BBa_K812020/annotation2187775/range2187775/1
type: Range
persistent identity: range2187775
display id: range2187775
version: 1
direction: direction/forward
top level: BBa_K812020
start: 1
end: 11
orientation: inline

IaaH
type: SequenceAnnotation
persistent identity: annotation2187776
display id: annotation2187776
version: 1
top level: BBa_K812020
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K812020/annotation2187776/range2187776/1

https://synbiohub.org/public/igem/BBa_K812020/annotation2187776/range2187776/1
type: Range
persistent identity: range2187776
display id: range2187776
version: 1
direction: direction/forward
top level: BBa_K812020
start: 12
end: 1355
orientation: inline

c2 P22
type: ComponentDefinition
persistent identity: BBa C0053
display id: BBa_C0053
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: c2 repressor from Salmonella phage P22 (+LVA)
creator: Maia Mahoney
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: The P22 c2 repressor protein coding sequence is a 720 base-pair sequence with the standard RBS-compatible BioBrick prefix and the standard BioBrick suffix sections on its ends. It binds to the P22 c2 regulatory sequence, BBa_R0053. The sequence contains a LVA tag for faster degredation.
mutable notes: References (unparsed) here: Vander Byl,C. and Kropinski,A.M. Sequence of the genome of Salmonella bacteriophage P22 J. Bacteriol. 182 (22), 6472-6481 (2000) References (unparsed) here: Vander Byl,C. and Kropinski,A.M. Sequence of the genome of Salmonella bacteriophage P22 J. Bacteriol. 182 (22), 6472-6481 (2000)
mutable provenance: Bacteriophage P22
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: BBa_C0053
sequence annotation: cII p22
sequence annotation: stop
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0053_sequence/1

BBa_C0053
type: SequenceAnnotation
persistent identity: annotation7036
display id: annotation7036
version: 1
top level: c2 P22
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_C0053/annotation7036/range7036/1

https://synbiohub.org/public/igem/BBa_C0053/annotation7036/range7036/1
type: Range
persistent identity: range7036
display id: range7036
version: 1
direction: direction/forward
top level: c2 P22
start: 1
end: 687
orientation: inline

cII p22
type: SequenceAnnotation
persistent identity: annotation1747
display id: annotation1747
version: 1
top level: c2 P22
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0053/annotation1747/range1747/1

https://synbiohub.org/public/igem/BBa_C0053/annotation1747/range1747/1
type: Range
persistent identity: range1747
display id: range1747
version: 1
direction: direction/forward
top level: c2 P22
start: 1
end: 648
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1751
display id: annotation1751
version: 1
top level: c2 P22
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_C0053/annotation1751/range1751/1

https://synbiohub.org/public/igem/BBa_C0053/annotation1751/range1751/1
type: Range
persistent identity: range1751
display id: range1751
version: 1
direction: direction/forward
top level: c2 P22
start: 682
end: 687
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation1750
display id: annotation1750
version: 1
top level: c2 P22
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_C0053/annotation1750/range1750/1

https://synbiohub.org/public/igem/BBa_C0053/annotation1750/range1750/1
type: Range
persistent identity: range1750
display id: range1750
version: 1
direction: direction/forward
top level: c2 P22
start: 649
end: 681
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213993
display id: annotation2213993
version: 1
top level: c2 P22
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_C0053/annotation2213993/range2213993/1

https://synbiohub.org/public/igem/BBa_C0053/annotation2213993/range2213993/1
type: Range
persistent identity: range2213993
display id: range2213993
version: 1
direction: direction/forward
top level: c2 P22
start: 688
end: 712
orientation: inline

fhlA-E363
type: ComponentDefinition
persistent identity: BBa K778002
display id: BBa_K778002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: fhlA-E363G (engineered trascriptional activator fhlA with a mutation)
creator: Yuka Yamazaki
created: 2012-09-21T11:00:00Z
modified: 2015-05-08T01:13:17Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1032_
m user id: 0
owner id: 13134
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: fhlA363
mutable notes: E363G
mutable provenance: BBa_K778001
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: stop
sequence annotation: FhlA-E363G
sequence annotation: A to G (different from E. coli K-12 MG1655)
sequence annotation: E363G mutation
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_K778002_sequence/1

stop
type: SequenceAnnotation
persistent identity: annotation2194870
display id: annotation2194870
version: 1
top level: fhlA-E363
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K778002/annotation2194870/range2194870/1

https://synbiohub.org/public/igem/BBa_K778002/annotation2194870/range2194870/1
type: Range
persistent identity: range2194870
display id: range2194870
version: 1
direction: direction/forward
top level: fhlA-E363
start: 2077
end: 2079
orientation: inline

FhlA-E363G
type: SequenceAnnotation
persistent identity: annotation2201467
display id: annotation2201467
version: 1
top level: fhlA-E363
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K778002/annotation2201467/range2201467/1

https://synbiohub.org/public/igem/BBa_K778002/annotation2201467/range2201467/1
type: Range
persistent identity: range2201467
display id: range2201467
version: 1
direction: direction/forward
top level: fhlA-E363
start: 1
end: 2079
orientation: inline

A to G (different from E. coli K-12 MG1655)
type: SequenceAnnotation
persistent identity: annotation2195764
display id: annotation2195764
version: 1
top level: fhlA-E363
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K778002/annotation2195764/range2195764/1

https://synbiohub.org/public/igem/BBa_K778002/annotation2195764/range2195764/1
type: Range
persistent identity: range2195764
display id: range2195764
version: 1
direction: direction/forward
top level: fhlA-E363
start: 1906
end: 1906
orientation: inline

E363G mutation
type: SequenceAnnotation
persistent identity: annotation2194869
display id: annotation2194869
version: 1
top level: fhlA-E363
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K778002/annotation2194869/range2194869/1

https://synbiohub.org/public/igem/BBa_K778002/annotation2194869/range2194869/1
type: Range
persistent identity: range2194869
display id: range2194869
version: 1
direction: direction/forward
top level: fhlA-E363
start: 1088
end: 1088
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2194868
display id: annotation2194868
version: 1
top level: fhlA-E363
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K778002/annotation2194868/range2194868/1

https://synbiohub.org/public/igem/BBa_K778002/annotation2194868/range2194868/1
type: Range
persistent identity: range2194868
display id: range2194868
version: 1
direction: direction/forward
top level: fhlA-E363
start: 1
end: 1
orientation: inline

BBa_J06602
type: ComponentDefinition
persistent identity: BBa J06602
display id: BBa_J06602
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Construction Intermediate: mCherry, bacterial with RBS (B0034.J06504)
creator: ytwang
created: 2005-06-13T11:00:00Z
modified: 2015-08-31T04:08:18Z
discontinued: false
dominant: true
group u list: _20_
m user id: 0
owner id: 340
owning group id: 20
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Combines BBa_B0034 RBS (Elowitz 1999) with BBa_J06504 mCherry, bacterial. This is an intermediate leading to BBa_J06702.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_J06504
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_J06504
sequence: https://synbiohub.org/public/igem/BBa_J06602_sequence/1

BBa_J06504
type: Component
persistent identity: component1596334
display id: component1596334
version: 1
top level: BBa_J06602
access: public
definition: mCherry

BBa_B0034
type: Component
persistent identity: component1596326
display id: component1596326
version: 1
top level: BBa_J06602
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1596326
display id: annotation1596326
version: 1
top level: BBa_J06602
location: https://synbiohub.org/public/igem/BBa_J06602/annotation1596326/range1596326/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J06602/annotation1596326/range1596326/1
type: Range
persistent identity: range1596326
display id: range1596326
version: 1
direction: direction/forward
top level: BBa_J06602
start: 1
end: 12
orientation: inline

BBa_J06504
type: SequenceAnnotation
persistent identity: annotation1596334
display id: annotation1596334
version: 1
top level: BBa_J06602
location: https://synbiohub.org/public/igem/BBa_J06602/annotation1596334/range1596334/1
component: BBa_J06504

https://synbiohub.org/public/igem/BBa_J06602/annotation1596334/range1596334/1
type: Range
persistent identity: range1596334
display id: range1596334
version: 1
direction: direction/forward
top level: BBa_J06602
start: 19
end: 732
orientation: inline

lux pR
type: ComponentDefinition
persistent identity: BBa R1062
display id: BBa_R1062
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter, Standard (luxR and HSL regulated -- lux pR)
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr Drew Endy
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: [Note: This is the same part as R0062 except that the -10 and -35 sites and spacing have been changed to comply with BBa_S0001]. Promoter activated by LuxR in concert with HSL The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the LuxR activator protein complexed with the autoinducer, 3-oxo-hexanoyl-HSL. Two molecules of LuxR protein form a complex with two molecules of the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
mutable notes: This promoter is based on the Vibrio fischeri quorum sensing gene promoters. Two genes LuxI and LuxR and transcribed in opposite directions as shown below. The original sequence from which the parts <bb_part>BBa_R0062</bb_part> and <bb_part>BBa_R0063</bb_part> were derived is shown in the picture below. <img src="<bb_file part=BBa_R0062>Image1.gif</bb_file>" width="614" height="362"> Modified to comply with BBa_S0001: TTGACA-17N-GATACT
mutable provenance: V. fischeri
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: start
sequence annotation: -35
sequence annotation: BBa_R1062
sequence annotation: -10
sequence annotation: LuxR/HSL
sequence: https://synbiohub.org/public/igem/BBa_R1062_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation2100
display id: annotation2100
version: 1
top level: lux pR
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_R1062/annotation2100/range2100/1

https://synbiohub.org/public/igem/BBa_R1062/annotation2100/range2100/1
type: Range
persistent identity: range2100
display id: range2100
version: 1
direction: direction/forward
top level: lux pR
start: 54
end: 54
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2101
display id: annotation2101
version: 1
top level: lux pR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R1062/annotation2101/range2101/1

https://synbiohub.org/public/igem/BBa_R1062/annotation2101/range2101/1
type: Range
persistent identity: range2101
display id: range2101
version: 1
direction: direction/forward
top level: lux pR
start: 20
end: 25
orientation: inline

BBa_R1062
type: SequenceAnnotation
persistent identity: annotation7077
display id: annotation7077
version: 1
top level: lux pR
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_R1062/annotation7077/range7077/1

https://synbiohub.org/public/igem/BBa_R1062/annotation7077/range7077/1
type: Range
persistent identity: range7077
display id: range7077
version: 1
direction: direction/forward
top level: lux pR
start: 1
end: 56
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2099
display id: annotation2099
version: 1
top level: lux pR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R1062/annotation2099/range2099/1

https://synbiohub.org/public/igem/BBa_R1062/annotation2099/range2099/1
type: Range
persistent identity: range2099
display id: range2099
version: 1
direction: direction/forward
top level: lux pR
start: 42
end: 48
orientation: inline

LuxR/HSL
type: SequenceAnnotation
persistent identity: annotation2098
display id: annotation2098
version: 1
top level: lux pR
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R1062/annotation2098/range2098/1

https://synbiohub.org/public/igem/BBa_R1062/annotation2098/range2098/1
type: Range
persistent identity: range2098
display id: range2098
version: 1
direction: direction/forward
top level: lux pR
start: 1
end: 20
orientation: inline

mRFP1
type: ComponentDefinition
persistent identity: BBa I13503
display id: BBa_I13503
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Screening plasmid intermediate
creator: jkm
created: 2005-05-30T11:00:00Z
modified: 2015-08-31T04:07:34Z
discontinued: false
dominant: true
experience: experience/None
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Built by Josh as an intermediate in screening plasmid construction
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_B0032
component: BBa_E1010
sequence annotation: BBa_B0032
sequence annotation: BBa_E1010
sequence: https://synbiohub.org/public/igem/BBa_I13503_sequence/1

BBa_B0032
type: Component
persistent identity: component1505053
display id: component1505053
version: 1
top level: mRFP1
access: public
definition: BBa_B0032

BBa_E1010
type: Component
persistent identity: component1505061
display id: component1505061
version: 1
top level: mRFP1
access: public
definition: https://synbiohub.org/public/igem/BBa_E1010/1

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1505053
display id: annotation1505053
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_I13503/annotation1505053/range1505053/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_I13503/annotation1505053/range1505053/1
type: Range
persistent identity: range1505053
display id: range1505053
version: 1
direction: direction/forward
top level: mRFP1
start: 1
end: 13
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation1505061
display id: annotation1505061
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_I13503/annotation1505061/range1505061/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_I13503/annotation1505061/range1505061/1
type: Range
persistent identity: range1505061
display id: range1505061
version: 1
direction: direction/forward
top level: mRFP1
start: 20
end: 700
orientation: inline

BBa_I13542
type: ComponentDefinition
persistent identity: BBa I13542
display id: BBa_I13542
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Intermediate in two promoter screening plasmid
creator: Josh Michener
created: 2005-08-21T11:00:00Z
modified: 2015-08-31T04:07:35Z
discontinued: false
dominant: false
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0012
component: BBa_B0010
component: BBa_B0034
component: BBa_E1010
component: BBa_I13457
component: BBa_I13453
sequence annotation: BBa_B0012
sequence annotation: BBa_E1010
sequence annotation: BBa_B0034
sequence annotation: BBa_I13457
sequence annotation: BBa_B0010
sequence annotation: BBa_I13453
sequence: https://synbiohub.org/public/igem/BBa_I13542_sequence/1

BBa_B0012
type: Component
persistent identity: component1663949
display id: component1663949
version: 1
top level: BBa_I13542
access: public
definition: BBa_B0012

BBa_B0010
type: Component
persistent identity: component1663939
display id: component1663939
version: 1
top level: BBa_I13542
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component1663926
display id: component1663926
version: 1
top level: BBa_I13542
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_E1010
type: Component
persistent identity: component1663933
display id: component1663933
version: 1
top level: BBa_I13542
access: public
definition: https://synbiohub.org/public/igem/BBa_E1010/1

BBa_I13457
type: Component
persistent identity: component1663921
display id: component1663921
version: 1
top level: BBa_I13542
access: public
definition: BBa_I13457

BBa_I13453
type: Component
persistent identity: component1663901
display id: component1663901
version: 1
top level: BBa_I13542
access: public
definition: BBa_I13453

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1663949
display id: annotation1663949
version: 1
top level: BBa_I13542
location: https://synbiohub.org/public/igem/BBa_I13542/annotation1663949/range1663949/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13542/annotation1663949/range1663949/1
type: Range
persistent identity: range1663949
display id: range1663949
version: 1
direction: direction/forward
top level: BBa_I13542
start: 1078
end: 1118
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation1663933
display id: annotation1663933
version: 1
top level: BBa_I13542
location: https://synbiohub.org/public/igem/BBa_I13542/annotation1663933/range1663933/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_I13542/annotation1663933/range1663933/1
type: Range
persistent identity: range1663933
display id: range1663933
version: 1
direction: direction/forward
top level: BBa_I13542
start: 276
end: 956
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1663926
display id: annotation1663926
version: 1
top level: BBa_I13542
location: https://synbiohub.org/public/igem/BBa_I13542/annotation1663926/range1663926/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13542/annotation1663926/range1663926/1
type: Range
persistent identity: range1663926
display id: range1663926
version: 1
direction: direction/forward
top level: BBa_I13542
start: 258
end: 269
orientation: inline

BBa_I13457
type: SequenceAnnotation
persistent identity: annotation1663921
display id: annotation1663921
version: 1
top level: BBa_I13542
location: https://synbiohub.org/public/igem/BBa_I13542/annotation1663921/range1663921/1
component: BBa_I13457

https://synbiohub.org/public/igem/BBa_I13542/annotation1663921/range1663921/1
type: Range
persistent identity: range1663921
display id: range1663921
version: 1
direction: direction/forward
top level: BBa_I13542
start: 139
end: 249
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1663939
display id: annotation1663939
version: 1
top level: BBa_I13542
location: https://synbiohub.org/public/igem/BBa_I13542/annotation1663939/range1663939/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13542/annotation1663939/range1663939/1
type: Range
persistent identity: range1663939
display id: range1663939
version: 1
direction: direction/forward
top level: BBa_I13542
start: 990
end: 1069
orientation: inline

BBa_I13453
type: SequenceAnnotation
persistent identity: annotation1663901
display id: annotation1663901
version: 1
top level: BBa_I13542
location: https://synbiohub.org/public/igem/BBa_I13542/annotation1663901/range1663901/1
component: BBa_I13453

https://synbiohub.org/public/igem/BBa_I13542/annotation1663901/range1663901/1
type: Range
persistent identity: range1663901
display id: range1663901
version: 1
direction: direction/forward
top level: BBa_I13542
start: 1
end: 130
orientation: inline

BBa_I6207
type: ComponentDefinition
persistent identity: BBa I6207
display id: BBa_I6207
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter O_H Test R0079.E0432
creator: Jennifer Braff
created: 2004-05-09T11:00:00Z
modified: 2015-08-31T04:07:44Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 60
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_E0032
component: BBa_B0010
component: BBa_B0034
component: BBa_R0079
component: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_E0032
sequence annotation: BBa_R0079
sequence: https://synbiohub.org/public/igem/BBa_I6207_sequence/1

BBa_E0032
type: Component
persistent identity: component950587
display id: component950587
version: 1
top level: BBa_I6207
access: public
definition: YFP

BBa_B0010
type: Component
persistent identity: component950595
display id: component950595
version: 1
top level: BBa_I6207
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component950575
display id: component950575
version: 1
top level: BBa_I6207
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0079
type: Component
persistent identity: component950570
display id: component950570
version: 1
top level: BBa_I6207
access: public
definition: LasR+PAI

BBa_B0012
type: Component
persistent identity: component950605
display id: component950605
version: 1
top level: BBa_I6207
access: public
definition: BBa_B0012

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation950575
display id: annotation950575
version: 1
top level: BBa_I6207
location: https://synbiohub.org/public/igem/BBa_I6207/annotation950575/range950575/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I6207/annotation950575/range950575/1
type: Range
persistent identity: range950575
display id: range950575
version: 1
direction: direction/forward
top level: BBa_I6207
start: 166
end: 177
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation950605
display id: annotation950605
version: 1
top level: BBa_I6207
location: https://synbiohub.org/public/igem/BBa_I6207/annotation950605/range950605/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I6207/annotation950605/range950605/1
type: Range
persistent identity: range950605
display id: range950605
version: 1
direction: direction/forward
top level: BBa_I6207
start: 1042
end: 1082
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation950595
display id: annotation950595
version: 1
top level: BBa_I6207
location: https://synbiohub.org/public/igem/BBa_I6207/annotation950595/range950595/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I6207/annotation950595/range950595/1
type: Range
persistent identity: range950595
display id: range950595
version: 1
direction: direction/forward
top level: BBa_I6207
start: 954
end: 1033
orientation: inline

BBa_E0032
type: SequenceAnnotation
persistent identity: annotation950587
display id: annotation950587
version: 1
top level: BBa_I6207
location: https://synbiohub.org/public/igem/BBa_I6207/annotation950587/range950587/1
component: BBa_E0032

https://synbiohub.org/public/igem/BBa_I6207/annotation950587/range950587/1
type: Range
persistent identity: range950587
display id: range950587
version: 1
direction: direction/forward
top level: BBa_I6207
start: 184
end: 945
orientation: inline

BBa_R0079
type: SequenceAnnotation
persistent identity: annotation950570
display id: annotation950570
version: 1
top level: BBa_I6207
location: https://synbiohub.org/public/igem/BBa_I6207/annotation950570/range950570/1
component: BBa_R0079

https://synbiohub.org/public/igem/BBa_I6207/annotation950570/range950570/1
type: Range
persistent identity: range950570
display id: range950570
version: 1
direction: direction/forward
top level: BBa_I6207
start: 1
end: 157
orientation: inline

BBa_I6064
type: ComponentDefinition
persistent identity: BBa I6064
display id: BBa_I6064
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter O_H Test R0050.E0432
creator: Caitlin Conboy and Jennifer Braff
created: 2004-03-17T12:00:00Z
modified: 2015-08-31T04:07:43Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_E0032
component: BBa_B0010
component: BBa_B0012
component: BBa_B0034
component: BBa_R0050
sequence annotation: BBa_B0034
sequence annotation: BBa_R0050
sequence annotation: BBa_E0032
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_I6064_sequence/1

BBa_E0032
type: Component
persistent identity: component949054
display id: component949054
version: 1
top level: BBa_I6064
access: public
definition: YFP

BBa_B0010
type: Component
persistent identity: component949062
display id: component949062
version: 1
top level: BBa_I6064
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component949072
display id: component949072
version: 1
top level: BBa_I6064
access: public
definition: BBa_B0012

BBa_B0034
type: Component
persistent identity: component949042
display id: component949042
version: 1
top level: BBa_I6064
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0050
type: Component
persistent identity: component949032
display id: component949032
version: 1
top level: BBa_I6064
access: public
definition: https://synbiohub.org/public/igem/BBa_R0050/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation949042
display id: annotation949042
version: 1
top level: BBa_I6064
location: https://synbiohub.org/public/igem/BBa_I6064/annotation949042/range949042/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I6064/annotation949042/range949042/1
type: Range
persistent identity: range949042
display id: range949042
version: 1
direction: direction/forward
top level: BBa_I6064
start: 64
end: 75
orientation: inline

BBa_R0050
type: SequenceAnnotation
persistent identity: annotation949032
display id: annotation949032
version: 1
top level: BBa_I6064
location: https://synbiohub.org/public/igem/BBa_I6064/annotation949032/range949032/1
component: BBa_R0050

https://synbiohub.org/public/igem/BBa_I6064/annotation949032/range949032/1
type: Range
persistent identity: range949032
display id: range949032
version: 1
direction: direction/forward
top level: BBa_I6064
start: 1
end: 55
orientation: inline

BBa_E0032
type: SequenceAnnotation
persistent identity: annotation949054
display id: annotation949054
version: 1
top level: BBa_I6064
location: https://synbiohub.org/public/igem/BBa_I6064/annotation949054/range949054/1
component: BBa_E0032

https://synbiohub.org/public/igem/BBa_I6064/annotation949054/range949054/1
type: Range
persistent identity: range949054
display id: range949054
version: 1
direction: direction/forward
top level: BBa_I6064
start: 82
end: 843
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation949072
display id: annotation949072
version: 1
top level: BBa_I6064
location: https://synbiohub.org/public/igem/BBa_I6064/annotation949072/range949072/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I6064/annotation949072/range949072/1
type: Range
persistent identity: range949072
display id: range949072
version: 1
direction: direction/forward
top level: BBa_I6064
start: 940
end: 980
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation949062
display id: annotation949062
version: 1
top level: BBa_I6064
location: https://synbiohub.org/public/igem/BBa_I6064/annotation949062/range949062/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I6064/annotation949062/range949062/1
type: Range
persistent identity: range949062
display id: range949062
version: 1
direction: direction/forward
top level: BBa_I6064
start: 852
end: 931
orientation: inline

BBa_K741008
type: ComponentDefinition
persistent identity: BBa K741008
display id: BBa_K741008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pCon(0.244)-RBS-crogfp-T
creator: Wuyang Chen
created: 2012-07-23T11:00:00Z
modified: 2015-05-08T01:13:09Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _990_
m user id: 0
owner id: 11656
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Here, pCon(J23114) constantly expressing the fusion protein crogfp at 10% of the strength of pCon(J23100).
mutable notes: TBD
mutable provenance: BBa_J23114 BBa_K741006
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K741006
component: BBa_J23105
sequence annotation: BBa_K741006
sequence annotation: BBa_J23105
sequence: https://synbiohub.org/public/igem/BBa_K741008_sequence/1

BBa_K741006
type: Component
persistent identity: component2223143
display id: component2223143
version: 1
top level: BBa_K741008
access: public
definition: https://synbiohub.org/public/igem/BBa_K741006/1

BBa_J23105
type: Component
persistent identity: component2223132
display id: component2223132
version: 1
top level: BBa_K741008
access: public
definition: https://synbiohub.org/public/igem/BBa_J23105/1

BBa_K741006
type: SequenceAnnotation
persistent identity: annotation2223143
display id: annotation2223143
version: 1
top level: BBa_K741008
location: https://synbiohub.org/public/igem/BBa_K741008/annotation2223143/range2223143/1
component: BBa_K741006

https://synbiohub.org/public/igem/BBa_K741008/annotation2223143/range2223143/1
type: Range
persistent identity: range2223143
display id: range2223143
version: 1
direction: direction/reverse
top level: BBa_K741008
start: 44
end: 1131
orientation: inline

BBa_J23105
type: SequenceAnnotation
persistent identity: annotation2223132
display id: annotation2223132
version: 1
top level: BBa_K741008
location: https://synbiohub.org/public/igem/BBa_K741008/annotation2223132/range2223132/1
component: BBa_J23105

https://synbiohub.org/public/igem/BBa_K741008/annotation2223132/range2223132/1
type: Range
persistent identity: range2223132
display id: range2223132
version: 1
direction: direction/reverse
top level: BBa_K741008
start: 1
end: 35
orientation: inline

BBa_I732810
type: ComponentDefinition
persistent identity: BBa I732810
display id: BBa_I732810
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ARL2A0203 (RBS+, TERM-)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:59Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_I732110
component: BBa_B0034
sequence annotation: BBa_I732110
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_I732810_sequence/1

BBa_I732110
type: Component
persistent identity: component1946193
display id: component1946193
version: 1
top level: BBa_I732810
access: public
definition: https://synbiohub.org/public/igem/BBa_I732110/1

BBa_B0034
type: Component
persistent identity: component1946192
display id: component1946192
version: 1
top level: BBa_I732810
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_I732110
type: SequenceAnnotation
persistent identity: annotation1946193
display id: annotation1946193
version: 1
top level: BBa_I732810
location: https://synbiohub.org/public/igem/BBa_I732810/annotation1946193/range1946193/1
component: BBa_I732110

https://synbiohub.org/public/igem/BBa_I732810/annotation1946193/range1946193/1
type: Range
persistent identity: range1946193
display id: range1946193
version: 1
direction: direction/reverse
top level: BBa_I732810
start: 19
end: 1104
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1946192
display id: annotation1946192
version: 1
top level: BBa_I732810
location: https://synbiohub.org/public/igem/BBa_I732810/annotation1946192/range1946192/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I732810/annotation1946192/range1946192/1
type: Range
persistent identity: range1946192
display id: range1946192
version: 1
direction: direction/reverse
top level: BBa_I732810
start: 1
end: 12
orientation: inline

araC-CysEm
type: ComponentDefinition
persistent identity: BBa K731030
display id: BBa_K731030
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Inducible araC-pBAD promoter regulating M256I CysE
creator: Jason Fontana, Daniele Rossetto
created: 2012-08-20T11:00:00Z
modified: 2015-05-08T01:13:06Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _977_
m user id: 0
owner id: 12113
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: The CysE M256I gene (K731010) is here regulated by the araC-pBAD promoter (K731200), which is inducible by arabinose.
mutable notes: K731200 and K731010 were inserted into pSB1C3 using a variation of the 3A Assembly, which added a PCR amplification step of the inserts before digestion to increase efficiency. The former was cut with E&S, the latter with X&P. pSB1C3 was digested with E&P.
mutable provenance: K731200, K731010
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K731010
component: BBa_K731201
sequence annotation: BBa_K731010
sequence annotation: BBa_K731201
sequence: https://synbiohub.org/public/igem/BBa_K731030_sequence/1

BBa_K731010
type: Component
persistent identity: component2331183
display id: component2331183
version: 1
top level: araC-CysEm
access: public
definition: https://synbiohub.org/public/igem/BBa_K731010/1

BBa_K731201
type: Component
persistent identity: component2331178
display id: component2331178
version: 1
top level: araC-CysEm
access: public
definition: https://synbiohub.org/public/igem/BBa_K731201/1

BBa_K731010
type: SequenceAnnotation
persistent identity: annotation2331183
display id: annotation2331183
version: 1
top level: araC-CysEm
location: https://synbiohub.org/public/igem/BBa_K731030/annotation2331183/range2331183/1
component: BBa_K731010

https://synbiohub.org/public/igem/BBa_K731030/annotation2331183/range2331183/1
type: Range
persistent identity: range2331183
display id: range2331183
version: 1
direction: direction/reverse
top level: araC-CysEm
start: 1212
end: 2050
orientation: inline

BBa_K731201
type: SequenceAnnotation
persistent identity: annotation2331178
display id: annotation2331178
version: 1
top level: araC-CysEm
location: https://synbiohub.org/public/igem/BBa_K731030/annotation2331178/range2331178/1
component: BBa_K731201

https://synbiohub.org/public/igem/BBa_K731030/annotation2331178/range2331178/1
type: Range
persistent identity: range2331178
display id: range2331178
version: 1
direction: direction/reverse
top level: araC-CysEm
start: 1
end: 1203
orientation: inline

BBa_K876009
type: ComponentDefinition
persistent identity: BBa K876009
display id: BBa_K876009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LuxR-DT-pLux-TetR
creator: Timothy Cogan
created: 2012-09-29T11:00:00Z
modified: 2015-05-08T01:13:38Z
discontinued: false
dominant: false
group u list: _1138_
m user id: 0
owner id: 13836
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Inducible promoter for TetR
mutable notes: Biobrick placement
mutable provenance: Generated from biobricks
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0015
component: BBa_C0040
component: BBa_R0062
component: BBa_C0062
sequence annotation: BBa_B0015
sequence annotation: BBa_C0040
sequence annotation: BBa_C0062
sequence annotation: BBa_R0062
sequence: https://synbiohub.org/public/igem/BBa_K876009_sequence/1

BBa_B0015
type: Component
persistent identity: component2259759
display id: component2259759
version: 1
top level: BBa_K876009
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_C0040
type: Component
persistent identity: component2259768
display id: component2259768
version: 1
top level: BBa_K876009
access: public
definition: tetR

BBa_R0062
type: Component
persistent identity: component2259761
display id: component2259761
version: 1
top level: BBa_K876009
access: public
definition: lux pR

BBa_C0062
type: Component
persistent identity: component2259749
display id: component2259749
version: 1
top level: BBa_K876009
access: public
definition: luxr

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2259759
display id: annotation2259759
version: 1
top level: BBa_K876009
location: https://synbiohub.org/public/igem/BBa_K876009/annotation2259759/range2259759/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K876009/annotation2259759/range2259759/1
type: Range
persistent identity: range2259759
display id: range2259759
version: 1
direction: direction/reverse
top level: BBa_K876009
start: 790
end: 918
orientation: inline

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation2259768
display id: annotation2259768
version: 1
top level: BBa_K876009
location: https://synbiohub.org/public/igem/BBa_K876009/annotation2259768/range2259768/1
component: BBa_C0040

https://synbiohub.org/public/igem/BBa_K876009/annotation2259768/range2259768/1
type: Range
persistent identity: range2259768
display id: range2259768
version: 1
direction: direction/reverse
top level: BBa_K876009
start: 988
end: 1672
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation2259749
display id: annotation2259749
version: 1
top level: BBa_K876009
location: https://synbiohub.org/public/igem/BBa_K876009/annotation2259749/range2259749/1
component: BBa_C0062

https://synbiohub.org/public/igem/BBa_K876009/annotation2259749/range2259749/1
type: Range
persistent identity: range2259749
display id: range2259749
version: 1
direction: direction/forward
top level: BBa_K876009
start: 1
end: 756
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation2259761
display id: annotation2259761
version: 1
top level: BBa_K876009
location: https://synbiohub.org/public/igem/BBa_K876009/annotation2259761/range2259761/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_K876009/annotation2259761/range2259761/1
type: Range
persistent identity: range2259761
display id: range2259761
version: 1
direction: direction/forward
top level: BBa_K876009
start: 927
end: 981
orientation: inline

OmpR
type: ComponentDefinition
persistent identity: BBa R0082
display id: BBa_R0082
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter (OmpR, positive)
creator: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
created: 2004-01-27T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Positively regulated, OmpR-controlled promoter. This promoter is taken from the upstream region of ompC. Phosphorylated OmpR binds to the three operator sites and activates transcription.
mutable notes: The promoter includes the three OmpR binding sites: C1, C2, and C3. The promoter starts at -107 and goes up to the transcriptional start site at +1. An alternate version, BBa_R0083, cuts out the C2 and C3 sites. The efficacy of this promoter versus the truncated ompC upstream region (BBa_R0083) or the ompF upstream region (BBa_R0084) is currently unknown.
mutable provenance: NC_000193 E. coli K12
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: C3 OmpR
sequence annotation: -10
sequence annotation: -35
sequence annotation: C1 OmpR
sequence annotation: C2 OmpR
sequence: https://synbiohub.org/public/igem/BBa_R0082_sequence/1

C3 OmpR
type: SequenceAnnotation
persistent identity: annotation301156
display id: annotation301156
version: 1
top level: OmpR
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301156/range301156/1

https://synbiohub.org/public/igem/BBa_R0082/annotation301156/range301156/1
type: Range
persistent identity: range301156
display id: range301156
version: 1
direction: direction/forward
top level: OmpR
start: 54
end: 71
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation301167
display id: annotation301167
version: 1
top level: OmpR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301167/range301167/1

https://synbiohub.org/public/igem/BBa_R0082/annotation301167/range301167/1
type: Range
persistent identity: range301167
display id: range301167
version: 1
direction: direction/forward
top level: OmpR
start: 98
end: 103
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation301166
display id: annotation301166
version: 1
top level: OmpR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301166/range301166/1

https://synbiohub.org/public/igem/BBa_R0082/annotation301166/range301166/1
type: Range
persistent identity: range301166
display id: range301166
version: 1
direction: direction/forward
top level: OmpR
start: 75
end: 80
orientation: inline

C1 OmpR
type: SequenceAnnotation
persistent identity: annotation301154
display id: annotation301154
version: 1
top level: OmpR
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301154/range301154/1

https://synbiohub.org/public/igem/BBa_R0082/annotation301154/range301154/1
type: Range
persistent identity: range301154
display id: range301154
version: 1
direction: direction/forward
top level: OmpR
start: 13
end: 30
orientation: inline

C2 OmpR
type: SequenceAnnotation
persistent identity: annotation301155
display id: annotation301155
version: 1
top level: OmpR
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301155/range301155/1

https://synbiohub.org/public/igem/BBa_R0082/annotation301155/range301155/1
type: Range
persistent identity: range301155
display id: range301155
version: 1
direction: direction/forward
top level: OmpR
start: 34
end: 51
orientation: inline

BBa_K847101
type: ComponentDefinition
persistent identity: BBa K847101
display id: BBa_K847101
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Engineered FliC with insertable expression region
creator: Julia Borden, Michelle Yu, Bella Okiddy
created: 2012-10-01T11:00:00Z
modified: 2015-05-08T01:13:34Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1107_
m user id: 0
owner id: 11092
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: TBA
mutable notes: TBA
mutable provenance: TBA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0030
component: BBa_K847102
component: BBa_B0015
component: BBa_J23100
sequence annotation: BBa_J23100
sequence annotation: BBa_B0015
sequence annotation: BBa_B0030
sequence annotation: BBa_K847102
sequence: https://synbiohub.org/public/igem/BBa_K847101_sequence/1

BBa_B0030
type: Component
persistent identity: component2209426
display id: component2209426
version: 1
top level: BBa_K847101
access: public
definition: BBa_B0030

BBa_K847102
type: Component
persistent identity: component2209431
display id: component2209431
version: 1
top level: BBa_K847101
access: public
definition: https://synbiohub.org/public/igem/BBa_K847102/1

BBa_B0015
type: Component
persistent identity: component2209438
display id: component2209438
version: 1
top level: BBa_K847101
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_J23100
type: Component
persistent identity: component2209424
display id: component2209424
version: 1
top level: BBa_K847101
access: public
definition: https://synbiohub.org/public/igem/BBa_J23100/1

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2209424
display id: annotation2209424
version: 1
top level: BBa_K847101
location: https://synbiohub.org/public/igem/BBa_K847101/annotation2209424/range2209424/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K847101/annotation2209424/range2209424/1
type: Range
persistent identity: range2209424
display id: range2209424
version: 1
direction: direction/reverse
top level: BBa_K847101
start: 1
end: 35
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2209438
display id: annotation2209438
version: 1
top level: BBa_K847101
location: https://synbiohub.org/public/igem/BBa_K847101/annotation2209438/range2209438/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K847101/annotation2209438/range2209438/1
type: Range
persistent identity: range2209438
display id: range2209438
version: 1
direction: direction/reverse
top level: BBa_K847101
start: 976
end: 1104
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2209426
display id: annotation2209426
version: 1
top level: BBa_K847101
location: https://synbiohub.org/public/igem/BBa_K847101/annotation2209426/range2209426/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K847101/annotation2209426/range2209426/1
type: Range
persistent identity: range2209426
display id: range2209426
version: 1
direction: direction/forward
top level: BBa_K847101
start: 44
end: 58
orientation: inline

BBa_K847102
type: SequenceAnnotation
persistent identity: annotation2209431
display id: annotation2209431
version: 1
top level: BBa_K847101
location: https://synbiohub.org/public/igem/BBa_K847101/annotation2209431/range2209431/1
component: BBa_K847102

https://synbiohub.org/public/igem/BBa_K847101/annotation2209431/range2209431/1
type: Range
persistent identity: range2209431
display id: range2209431
version: 1
direction: direction/reverse
top level: BBa_K847101
start: 65
end: 967
orientation: inline

BBa_K737033
type: ComponentDefinition
persistent identity: BBa K737033
display id: BBa_K737033
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: galK is the leader sequence of galactokinase (GalK)
creator: Peiran Zhang
created: 2012-09-17T11:00:00Z
modified: 2015-05-08T01:13:07Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _986_
m user id: 0
owner id: 14291
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: galK is the leader sequence of galactokinase (GalK),and Spot42 sRNA controls the synthesis of the galactokinase (GalK) in response to the availability of glucose in the environment,by blocking the recognition of antiSD motif to RBS.The repression is modest(about 2.6 fold),maybe there is no subsequent degradation caused by the major degradosome,RNaesE and RNaseIII. Note:The ClaI site is blocked by GATC methylation due to a mistake.It should be amplified by PCR to get rid of dam methylation before ClaI degestion
mutable notes: No
mutable provenance: Reference: [1] Johannes H. Urban and Jo?? rg Vogel, Translational control and target recognition by Escherichia coli small RNAs in vivo, Nucleic Acids Research, 2007, Vol. 35, No. 3 [2] Boris G??rke and J??rg Vogel, Noncoding RNA control of the making and breaking of sugars, GENES & DEVELOPMENT 22:2914???2925 ,2008
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_K737033_sequence/1

AraC
type: ComponentDefinition
persistent identity: BBa R0080
display id: BBa_R0080
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter (AraC regulated)
creator: Sara Neves (Fighting Darwins)
created: 2004-01-27T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: AraC operator, truncated to include araO1, araI1, araI2, c-amp1, and c-amp2 sites. This operator should *activate* transcription in the presence of AraC; b/c the operator lacks the araO2 site, there should not be araC-mediated repression.
mutable provenance: GenBank: J01641 (www.ncbi.nlm.nih.gov)
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: c-amp1
sequence annotation: -10
sequence annotation: c-amp2
sequence annotation: -35
sequence annotation: araO1
sequence annotation: ara1 and ara2
sequence: https://synbiohub.org/public/igem/BBa_R0080_sequence/1

c-amp1
type: SequenceAnnotation
persistent identity: annotation301458
display id: annotation301458
version: 1
top level: AraC
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0080/annotation301458/range301458/1

https://synbiohub.org/public/igem/BBa_R0080/annotation301458/range301458/1
type: Range
persistent identity: range301458
display id: range301458
version: 1
direction: direction/forward
top level: AraC
start: 43
end: 72
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation308602
display id: annotation308602
version: 1
top level: AraC
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0080/annotation308602/range308602/1

https://synbiohub.org/public/igem/BBa_R0080/annotation308602/range308602/1
type: Range
persistent identity: range308602
display id: range308602
version: 1
direction: direction/forward
top level: AraC
start: 136
end: 141
orientation: inline

c-amp2
type: SequenceAnnotation
persistent identity: annotation301456
display id: annotation301456
version: 1
top level: AraC
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0080/annotation301456/range301456/1

https://synbiohub.org/public/igem/BBa_R0080/annotation301456/range301456/1
type: Range
persistent identity: range301456
display id: range301456
version: 1
direction: direction/forward
top level: AraC
start: 4
end: 29
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation308601
display id: annotation308601
version: 1
top level: AraC
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0080/annotation308601/range308601/1

https://synbiohub.org/public/igem/BBa_R0080/annotation308601/range308601/1
type: Range
persistent identity: range308601
display id: range308601
version: 1
direction: direction/forward
top level: AraC
start: 113
end: 118
orientation: inline

araO1
type: SequenceAnnotation
persistent identity: annotation301457
display id: annotation301457
version: 1
top level: AraC
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_R0080/annotation301457/range301457/1

https://synbiohub.org/public/igem/BBa_R0080/annotation301457/range301457/1
type: Range
persistent identity: range301457
display id: range301457
version: 1
direction: direction/forward
top level: AraC
start: 6
end: 44
orientation: inline

ara1 and ara2
type: SequenceAnnotation
persistent identity: annotation301462
display id: annotation301462
version: 1
top level: AraC
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0080/annotation301462/range301462/1

https://synbiohub.org/public/igem/BBa_R0080/annotation301462/range301462/1
type: Range
persistent identity: range301462
display id: range301462
version: 1
direction: direction/forward
top level: AraC
start: 73
end: 101
orientation: inline

cinR
type: ComponentDefinition
persistent identity: BBa R0077
display id: BBa_R0077
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter (cinR and HSL regulated, RBS+)
creator: crackdots
created: 2004-01-27T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: CinR (BBa_C0077) in complex with O3-C14:1-HSL (from CinI, BBa_C0076) binds to this promoter and activates transcription. This promoter contains the RBS: TGGAGG
mutable notes: The regulatory locus cinRI in Rhizobium leguminosarum conrols a network of quorum-sensing loci Lithgow, JK; Wilkinson, A; Hardman, A; Rodelas, B; Wisniewski-Dye, F; Williams, P; Downie, AJ MOL. MICROBIOLOGY 37(1): 81-97, 2000 Change log: the last 5 bases (gctaa) removed to allow for the correct spacing between the RBS (TGGAGG) and the start of the downstream gene after biobricks splicing.
mutable provenance: Rhizobium leguminosarum
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: unidentified/uncharacterized CinR dependent
sequence annotation: stem_loop
sequence annotation: embedded RBS
sequence: https://synbiohub.org/public/igem/BBa_R0077_sequence/1

unidentified/uncharacterized CinR dependent
type: SequenceAnnotation
persistent identity: annotation301371
display id: annotation301371
version: 1
top level: cinR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0077/annotation301371/range301371/1

https://synbiohub.org/public/igem/BBa_R0077/annotation301371/range301371/1
type: Range
persistent identity: range301371
display id: range301371
version: 1
direction: direction/forward
top level: cinR
start: 1
end: 220
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation301205
display id: annotation301205
version: 1
top level: cinR
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_R0077/annotation301205/range301205/1

https://synbiohub.org/public/igem/BBa_R0077/annotation301205/range301205/1
type: Range
persistent identity: range301205
display id: range301205
version: 1
direction: direction/forward
top level: cinR
start: 91
end: 136
orientation: inline

embedded RBS
type: SequenceAnnotation
persistent identity: annotation301150
display id: annotation301150
version: 1
top level: cinR
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_R0077/annotation301150/range301150/1

https://synbiohub.org/public/igem/BBa_R0077/annotation301150/range301150/1
type: Range
persistent identity: range301150
display id: range301150
version: 1
direction: direction/forward
top level: cinR
start: 226
end: 231
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation301204
display id: annotation301204
version: 1
top level: cinR
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_R0077/annotation301204/range301204/1

https://synbiohub.org/public/igem/BBa_R0077/annotation301204/range301204/1
type: Range
persistent identity: range301204
display id: range301204
version: 1
direction: direction/forward
top level: cinR
start: 35
end: 74
orientation: inline

BBa_K909014
type: ComponentDefinition
persistent identity: BBa K909014
display id: BBa_K909014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Constitutive expression of pABA
creator: Deborah Huber
created: 2012-09-21T11:00:00Z
modified: 2015-05-08T01:13:45Z
discontinued: false
dominant: false
group u list: _1174_
m user id: 0
owner id: 14088
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: PABA is derived from the aromatic branch point precursor chorismate in two steps. Aminodeoxychorismate (ADC) synthase converts chorismate and glutamine to ADC and glutamate. The conversion of chorismate and glutamine to ADC and glutamate determines the rate and therefore overexpression of ADC synthase leads to overproduction of pABA. ADC synthase is composed of two subunits, PabA and PabB. Finally ADC lyase removes pyruvate from ADC, aromatizes the ring, and generates PABA.
mutable notes: xyz
mutable provenance: Kinetic characterization of 4-amino 4-deoxychorismate synthase from Escherichia coli. V K Viswanathan,; J M Green and; B P Nichols.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0034
component: BBa_B0010
component: BBa_B0012
component: BBa_K137005
component: BBa_K137006
component: BBa_J23100
sequence annotation: BBa_B0034
sequence annotation: BBa_K137005
sequence annotation: BBa_K137006
sequence annotation: BBa_B0012
sequence annotation: BBa_J23100
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_K909014_sequence/1

BBa_B0034
type: Component
persistent identity: component2193595
display id: component2193595
version: 1
top level: BBa_K909014
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: Component
persistent identity: component2193592
display id: component2193592
version: 1
top level: BBa_K909014
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component2193597
display id: component2193597
version: 1
top level: BBa_K909014
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component2193599
display id: component2193599
version: 1
top level: BBa_K909014
access: public
definition: BBa_B0012

BBa_K137005
type: Component
persistent identity: component2193596
display id: component2193596
version: 1
top level: BBa_K909014
access: public
definition: https://synbiohub.org/public/igem/BBa_K137005/1

BBa_K137006
type: Component
persistent identity: component2193593
display id: component2193593
version: 1
top level: BBa_K909014
access: public
definition: https://synbiohub.org/public/igem/BBa_K137006/1

BBa_J23100
type: Component
persistent identity: component2193590
display id: component2193590
version: 1
top level: BBa_K909014
access: public
definition: https://synbiohub.org/public/igem/BBa_J23100/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2193595
display id: annotation2193595
version: 1
top level: BBa_K909014
location: https://synbiohub.org/public/igem/BBa_K909014/annotation2193595/range2193595/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K909014/annotation2193595/range2193595/1
type: Range
persistent identity: range2193595
display id: range2193595
version: 1
direction: direction/reverse
top level: BBa_K909014
start: 1960
end: 1971
orientation: inline

BBa_K137005
type: SequenceAnnotation
persistent identity: annotation2193596
display id: annotation2193596
version: 1
top level: BBa_K909014
location: https://synbiohub.org/public/igem/BBa_K909014/annotation2193596/range2193596/1
component: BBa_K137005

https://synbiohub.org/public/igem/BBa_K909014/annotation2193596/range2193596/1
type: Range
persistent identity: range2193596
display id: range2193596
version: 1
direction: direction/reverse
top level: BBa_K909014
start: 1978
end: 2562
orientation: inline

BBa_K137006
type: SequenceAnnotation
persistent identity: annotation2193593
display id: annotation2193593
version: 1
top level: BBa_K909014
location: https://synbiohub.org/public/igem/BBa_K909014/annotation2193593/range2193593/1
component: BBa_K137006

https://synbiohub.org/public/igem/BBa_K909014/annotation2193593/range2193593/1
type: Range
persistent identity: range2193593
display id: range2193593
version: 1
direction: direction/reverse
top level: BBa_K909014
start: 62
end: 1951
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2193599
display id: annotation2193599
version: 1
top level: BBa_K909014
location: https://synbiohub.org/public/igem/BBa_K909014/annotation2193599/range2193599/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K909014/annotation2193599/range2193599/1
type: Range
persistent identity: range2193599
display id: range2193599
version: 1
direction: direction/forward
top level: BBa_K909014
start: 2659
end: 2699
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2193592
display id: annotation2193592
version: 1
top level: BBa_K909014
location: https://synbiohub.org/public/igem/BBa_K909014/annotation2193592/range2193592/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K909014/annotation2193592/range2193592/1
type: Range
persistent identity: range2193592
display id: range2193592
version: 1
direction: direction/reverse
top level: BBa_K909014
start: 44
end: 55
orientation: inline

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2193590
display id: annotation2193590
version: 1
top level: BBa_K909014
location: https://synbiohub.org/public/igem/BBa_K909014/annotation2193590/range2193590/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K909014/annotation2193590/range2193590/1
type: Range
persistent identity: range2193590
display id: range2193590
version: 1
direction: direction/reverse
top level: BBa_K909014
start: 1
end: 35
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2193597
display id: annotation2193597
version: 1
top level: BBa_K909014
location: https://synbiohub.org/public/igem/BBa_K909014/annotation2193597/range2193597/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K909014/annotation2193597/range2193597/1
type: Range
persistent identity: range2193597
display id: range2193597
version: 1
direction: direction/forward
top level: BBa_K909014
start: 2571
end: 2650
orientation: inline

BBa_K876060
type: ComponentDefinition
persistent identity: BBa K876060
display id: BBa_K876060
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Term-Term-plasR-RBS-CFP-Term-Term
creator: Leah Ferrell, Sanket Shah, Shayan Ghassemi, Zakaraya Ashour
created: 2012-10-01T11:00:00Z
modified: 2015-05-08T01:13:39Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1138_
m user id: 0
owner id: 13835
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: pLasR is off by default and can be activated by LasR.
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_R0079
component: BBa_E0422
component: BBa_B0015
sequence annotation: BBa_B0015
sequence annotation: BBa_R0079
sequence annotation: BBa_E0422
sequence: https://synbiohub.org/public/igem/BBa_K876060_sequence/1

BBa_R0079
type: Component
persistent identity: component2206368
display id: component2206368
version: 1
top level: BBa_K876060
access: public
definition: LasR+PAI

BBa_E0422
type: Component
persistent identity: component2206382
display id: component2206382
version: 1
top level: BBa_K876060
access: public
definition: BBa_E0422

BBa_B0015
type: Component
persistent identity: component2206363
display id: component2206363
version: 1
top level: BBa_K876060
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2206363
display id: annotation2206363
version: 1
top level: BBa_K876060
location: https://synbiohub.org/public/igem/BBa_K876060/annotation2206363/range2206363/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K876060/annotation2206363/range2206363/1
type: Range
persistent identity: range2206363
display id: range2206363
version: 1
direction: direction/reverse
top level: BBa_K876060
start: 1
end: 129
orientation: inline

BBa_R0079
type: SequenceAnnotation
persistent identity: annotation2206368
display id: annotation2206368
version: 1
top level: BBa_K876060
location: https://synbiohub.org/public/igem/BBa_K876060/annotation2206368/range2206368/1
component: BBa_R0079

https://synbiohub.org/public/igem/BBa_K876060/annotation2206368/range2206368/1
type: Range
persistent identity: range2206368
display id: range2206368
version: 1
direction: direction/reverse
top level: BBa_K876060
start: 138
end: 294
orientation: inline

BBa_E0422
type: SequenceAnnotation
persistent identity: annotation2206382
display id: annotation2206382
version: 1
top level: BBa_K876060
location: https://synbiohub.org/public/igem/BBa_K876060/annotation2206382/range2206382/1
component: BBa_E0422

https://synbiohub.org/public/igem/BBa_K876060/annotation2206382/range2206382/1
type: Range
persistent identity: range2206382
display id: range2206382
version: 1
direction: direction/reverse
top level: BBa_K876060
start: 303
end: 1219
orientation: inline

BBa_I715039
type: ComponentDefinition
persistent identity: BBa I715039
display id: BBa_I715039
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pLac-RBS-RFP
creator: Oyinade Adefuye
created: 2007-07-31T11:00:00Z
modified: 2015-08-31T04:07:49Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _120_
m user id: 0
owner id: 1523
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: RBS-1Chlora
mutable notes: RBS-1Chlora
mutable provenance: RBS-1Chlora
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_R0010
component: BBa_I13502
sequence annotation: BBa_R0010
sequence annotation: BBa_I13502
sequence: https://synbiohub.org/public/igem/BBa_I715039_sequence/1

BBa_R0010
type: Component
persistent identity: component2245597
display id: component2245597
version: 1
top level: BBa_I715039
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_I13502
type: Component
persistent identity: component2245609
display id: component2245609
version: 1
top level: BBa_I715039
access: public
definition: mRFP1

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2245597
display id: annotation2245597
version: 1
top level: BBa_I715039
location: https://synbiohub.org/public/igem/BBa_I715039/annotation2245597/range2245597/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_I715039/annotation2245597/range2245597/1
type: Range
persistent identity: range2245597
display id: range2245597
version: 1
direction: direction/forward
top level: BBa_I715039
start: 1
end: 200
orientation: inline

BBa_I13502
type: SequenceAnnotation
persistent identity: annotation2245609
display id: annotation2245609
version: 1
top level: BBa_I715039
location: https://synbiohub.org/public/igem/BBa_I715039/annotation2245609/range2245609/1
component: BBa_I13502

https://synbiohub.org/public/igem/BBa_I715039/annotation2245609/range2245609/1
type: Range
persistent identity: range2245609
display id: range2245609
version: 1
direction: direction/reverse
top level: BBa_I715039
start: 209
end: 932
orientation: inline

BBa_K876010
type: ComponentDefinition
persistent identity: BBa K876010
display id: BBa_K876010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pLux-TetR
creator: Timothy Cogan
created: 2012-09-29T11:00:00Z
modified: 2015-05-08T01:13:38Z
discontinued: false
dominant: false
group u list: _1138_
m user id: 0
owner id: 13836
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Inducible promoter for TetR
mutable notes: Biobrick placement
mutable provenance: Generated from biobricks
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_R0062
component: BBa_C0040
sequence annotation: BBa_C0040
sequence annotation: BBa_R0062
sequence: https://synbiohub.org/public/igem/BBa_K876010_sequence/1

BBa_R0062
type: Component
persistent identity: component2245097
display id: component2245097
version: 1
top level: BBa_K876010
access: public
definition: lux pR

BBa_C0040
type: Component
persistent identity: component2245105
display id: component2245105
version: 1
top level: BBa_K876010
access: public
definition: tetR

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation2245105
display id: annotation2245105
version: 1
top level: BBa_K876010
location: https://synbiohub.org/public/igem/BBa_K876010/annotation2245105/range2245105/1
component: BBa_C0040

https://synbiohub.org/public/igem/BBa_K876010/annotation2245105/range2245105/1
type: Range
persistent identity: range2245105
display id: range2245105
version: 1
direction: direction/reverse
top level: BBa_K876010
start: 62
end: 746
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation2245097
display id: annotation2245097
version: 1
top level: BBa_K876010
location: https://synbiohub.org/public/igem/BBa_K876010/annotation2245097/range2245097/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_K876010/annotation2245097/range2245097/1
type: Range
persistent identity: range2245097
display id: range2245097
version: 1
direction: direction/forward
top level: BBa_K876010
start: 1
end: 55
orientation: inline

BBa_J202007
type: ComponentDefinition
persistent identity: BBa J202007
display id: BBa_J202007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PBAD Mutnat#8
creator: Jiyeon Park
created: 2012-07-25T11:00:00Z
modified: 2015-08-31T04:08:37Z
discontinued: false
dominant: false
group u list: _910_
m user id: 0
owner id: 11164
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is PBAD (BBa_I13453) mutant. -62 relative to the araC transcriptional start site was changed from A to G.
mutable notes: In order to regulate transcription from PBAD, araC binds three different sites called araO2, araI1, and araI2. In the absence of L-arabinose, araC binds both araO2 and araI1 and bends DNA preventing transcription from PBAD. In the presence of L-arabinose, however, araC has a conformational change because of L-arabinose and binds araI1 and araI2, enabling transcription from PBAD. Like the wild-type (I13453), this part does not have araO2 site which is essential for araC repressor function. Therefore, araC acts as an activator in the presence of L-arabinose.
mutable provenance: This mutant was created by site-directed mutagenesis.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: araI1
sequence annotation: Mutation
sequence annotation: Transcription Start
sequence annotation: araI2
sequence: https://synbiohub.org/public/igem/BBa_J202007_sequence/1

araI1
type: SequenceAnnotation
persistent identity: annotation2178445
display id: annotation2178445
version: 1
top level: BBa_J202007
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J202007/annotation2178445/range2178445/1

https://synbiohub.org/public/igem/BBa_J202007/annotation2178445/range2178445/1
type: Range
persistent identity: range2178445
display id: range2178445
version: 1
direction: direction/forward
top level: BBa_J202007
start: 40
end: 56
orientation: inline

Mutation
type: SequenceAnnotation
persistent identity: annotation2178447
display id: annotation2178447
version: 1
top level: BBa_J202007
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_J202007/annotation2178447/range2178447/1

https://synbiohub.org/public/igem/BBa_J202007/annotation2178447/range2178447/1
type: Range
persistent identity: range2178447
display id: range2178447
version: 1
direction: direction/forward
top level: BBa_J202007
start: 50
end: 50
orientation: inline

Transcription Start
type: SequenceAnnotation
persistent identity: annotation2178444
display id: annotation2178444
version: 1
top level: BBa_J202007
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_J202007/annotation2178444/range2178444/1

https://synbiohub.org/public/igem/BBa_J202007/annotation2178444/range2178444/1
type: Range
persistent identity: range2178444
display id: range2178444
version: 1
direction: direction/forward
top level: BBa_J202007
start: 112
end: 112
orientation: inline

araI2
type: SequenceAnnotation
persistent identity: annotation2178446
display id: annotation2178446
version: 1
top level: BBa_J202007
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J202007/annotation2178446/range2178446/1

https://synbiohub.org/public/igem/BBa_J202007/annotation2178446/range2178446/1
type: Range
persistent identity: range2178446
display id: range2178446
version: 1
direction: direction/forward
top level: BBa_J202007
start: 61
end: 77
orientation: inline

BBa_I14044
type: ComponentDefinition
persistent identity: BBa I14044
display id: BBa_I14044
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: weak RBS . LacI
creator: Aditi Shrivastava, Jiwon Lee, Sarah Welch
created: 2004-08-03T11:00:00Z
modified: 2015-08-31T04:07:38Z
discontinued: false
dominant: false
experience: experience/None
group u list: _4_
m user id: 0
owner id: 166
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: BBa_B0031 BBa_C0012
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_C0012
component: BBa_B0031
sequence annotation: BBa_C0012
sequence annotation: BBa_B0031
sequence: https://synbiohub.org/public/igem/BBa_I14044_sequence/1

BBa_C0012
type: Component
persistent identity: component1041746
display id: component1041746
version: 1
top level: BBa_I14044
access: public
definition: lacI

BBa_B0031
type: Component
persistent identity: component1041736
display id: component1041736
version: 1
top level: BBa_I14044
access: public
definition: BBa_B0031

BBa_C0012
type: SequenceAnnotation
persistent identity: annotation1041746
display id: annotation1041746
version: 1
top level: BBa_I14044
location: https://synbiohub.org/public/igem/BBa_I14044/annotation1041746/range1041746/1
component: BBa_C0012

https://synbiohub.org/public/igem/BBa_I14044/annotation1041746/range1041746/1
type: Range
persistent identity: range1041746
display id: range1041746
version: 1
direction: direction/forward
top level: BBa_I14044
start: 21
end: 1148
orientation: inline

BBa_B0031
type: SequenceAnnotation
persistent identity: annotation1041736
display id: annotation1041736
version: 1
top level: BBa_I14044
location: https://synbiohub.org/public/igem/BBa_I14044/annotation1041736/range1041736/1
component: BBa_B0031

https://synbiohub.org/public/igem/BBa_I14044/annotation1041736/range1041736/1
type: Range
persistent identity: range1041736
display id: range1041736
version: 1
direction: direction/forward
top level: BBa_I14044
start: 1
end: 14
orientation: inline

BBa_K850002
type: ComponentDefinition
persistent identity: BBa K850002
display id: BBa_K850002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: UAP1 gene from candida albicans
creator: Min-Gyu Kim
created: 2012-09-14T11:00:00Z
modified: 2015-05-08T01:13:34Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1110_
m user id: 0
owner id: 10645
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: UDP-N-acetylglucosamine pyrophosphorylase, catalyzes biosynthesis of UDP-N-acetylglucosamine from UTP and N-acetylglucosamine 1-phosphate; functional homolog of S. cerevisiae Qri1p; alkaline upregulated. This gene is part of the pathway that synthesizes the sugar subunit of the polymer chitin.
mutable notes: Part was synthesized from <500bp overlapping Gibson Assembly fragments
mutable provenance: candida genome database orf19.4265 (C. albicans SC5314)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K850002_sequence/1

cI 434
type: ComponentDefinition
persistent identity: BBa C0052
display id: BBa_C0052
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cI repressor from phage 434 (+LVA)
creator: Maia Mahoney
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: The 434 cI repressor protein coding sequence is a 710 base-pair sequence with the standard RBS-compatible BioBrick prefix and the standard BioBrick suffix sections on its ends. It binds to the 434 regulatory sequence, BBa_R0052. The sequence contains a LVA tag for faster degredation and has no RBS.
mutable notes: References (unparsed) here: Nikolnikov,S., Posfai,G. and Sain,B. The construction of a versatile plasmid vector that allows direct selection of fragments cloned into six unique sites of the cI gene of coliphage 434. Gene 30 (1-3), 261-265 (1984) References (unparsed) here: Nikolnikov,S., Posfai,G. and Sain,B. The construction of a versatile plasmid vector that allows direct selection of fragments cloned into six unique sites of the cI gene of coliphage 434. Gene 30 (1-3), 261-265 (1984)
mutable provenance: Bacteriophage 434
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: BBa_C0052
sequence annotation: Help:Barcodes
sequence annotation: LVA
sequence annotation: cI 434
sequence: https://synbiohub.org/public/igem/BBa_C0052_sequence/1

BBa_C0052
type: SequenceAnnotation
persistent identity: annotation7035
display id: annotation7035
version: 1
top level: cI 434
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_C0052/annotation7035/range7035/1

https://synbiohub.org/public/igem/BBa_C0052/annotation7035/range7035/1
type: Range
persistent identity: range7035
display id: range7035
version: 1
direction: direction/forward
top level: cI 434
start: 1
end: 669
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213992
display id: annotation2213992
version: 1
top level: cI 434
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_C0052/annotation2213992/range2213992/1

https://synbiohub.org/public/igem/BBa_C0052/annotation2213992/range2213992/1
type: Range
persistent identity: range2213992
display id: range2213992
version: 1
direction: direction/forward
top level: cI 434
start: 670
end: 694
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation1745
display id: annotation1745
version: 1
top level: cI 434
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_C0052/annotation1745/range1745/1

https://synbiohub.org/public/igem/BBa_C0052/annotation1745/range1745/1
type: Range
persistent identity: range1745
display id: range1745
version: 1
direction: direction/forward
top level: cI 434
start: 631
end: 669
orientation: inline

cI 434
type: SequenceAnnotation
persistent identity: annotation1743
display id: annotation1743
version: 1
top level: cI 434
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0052/annotation1743/range1743/1

https://synbiohub.org/public/igem/BBa_C0052/annotation1743/range1743/1
type: Range
persistent identity: range1743
display id: range1743
version: 1
direction: direction/forward
top level: cI 434
start: 1
end: 669
orientation: inline

BBa_K895005
type: ComponentDefinition
persistent identity: BBa K895005
display id: BBa_K895005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Salty_VgrG-CD27_endolysin
creator: Frank Sargent
created: 2012-09-22T11:00:00Z
modified: 2015-05-08T01:13:41Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _1160_
m user id: 0
owner id: 8083
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is a coding part comprising the full open reading frame of Salmonella enterica LT2 vgrG gene (also known as STM0289 or tssI) linked via an haemagglutinin eptitope tag to a synthetic gene encoding an endolysin from the CD27 bacteriophage. The CD27 endolysin is believed to have high specificity against the cell wall of Clostridium difficile.
mutable notes: None.
mutable provenance: The vgrG section is derived from BBa_K895002 , which was used as a PCR template, and the endolysin section is derived from a synthetic gene designed by Neil Fairweather's group at Imperial College London.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: HA tag
sequence annotation: endolysin
sequence annotation: vgrG
sequence: https://synbiohub.org/public/igem/BBa_K895005_sequence/1

HA tag
type: SequenceAnnotation
persistent identity: annotation2194045
display id: annotation2194045
version: 1
top level: BBa_K895005
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K895005/annotation2194045/range2194045/1

https://synbiohub.org/public/igem/BBa_K895005/annotation2194045/range2194045/1
type: Range
persistent identity: range2194045
display id: range2194045
version: 1
direction: direction/forward
top level: BBa_K895005
start: 2188
end: 2214
orientation: inline

endolysin
type: SequenceAnnotation
persistent identity: annotation2194046
display id: annotation2194046
version: 1
top level: BBa_K895005
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K895005/annotation2194046/range2194046/1

https://synbiohub.org/public/igem/BBa_K895005/annotation2194046/range2194046/1
type: Range
persistent identity: range2194046
display id: range2194046
version: 1
direction: direction/forward
top level: BBa_K895005
start: 2221
end: 2793
orientation: inline

vgrG
type: SequenceAnnotation
persistent identity: annotation2194044
display id: annotation2194044
version: 1
top level: BBa_K895005
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K895005/annotation2194044/range2194044/1

https://synbiohub.org/public/igem/BBa_K895005/annotation2194044/range2194044/1
type: Range
persistent identity: range2194044
display id: range2194044
version: 1
direction: direction/forward
top level: BBa_K895005
start: 1
end: 2187
orientation: inline

BBa_I746104
type: ComponentDefinition
persistent identity: BBa I746104
display id: BBa_I746104
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: P2 promoter in agr operon from S. aureus
creator: Zhizhen Zhao
created: 2007-08-30T11:00:00Z
modified: 2015-08-31T04:08:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _116_
m user id: 0
owner id: 2121
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: The agr P2 operon is an autocatalytic sensory transduction system in Staphylococcus aureus. P2 promoter regulates the synthesis of AgrB (a transmembrane protein) and AgrD (precursor of AIP autoinducing peptide). AIP binds to AgrC, a membrane binding receptor and it phosphorylates AgrA. The phosphorylated AgrA increases the expression level from P2 promoter.
mutable notes: Codon usage has been optimised for E. coli (which also seems to give good results for B. subtilis
mutable provenance: The section of the sequence constituting the P2 promoter is subject to rather more debate than the sequences of the coding regions. [http://jb.asm.org/cgi/content/full/186/22/7549 Koenig, Robbin L., Ray, Jessica L., Maleki, Soheila J., Smeltzer, Mark S., Hurlburt, Barry K. Staphylococcus aureus AgrA Binding to the RNAIII-agr Regulatory Region J. Bacteriol. 2004 186: 7549-7555] shows the areas of the sequence to which phosphorylated AgrA binds while L Rao, R K Karls, and M J Betley: In vitro transcription of pathogenesis-related genes by purified RNA polymerase from Staphylococcus aureus. Bacteriol. 1995 May; 177(10): 2609???2614. shows the transcription start sites; we took the overall extent of the promoter region from that labelled in Morfeldt, E., K. Tegmark, and S. Arvidson. 1996. Transcriptional control of the agr-dependent virulence gene regulator, RNAIII, in Staphylococcus aureus. Mol. Microbiol. 21:1227???1237. (not available online).
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I746104_sequence/1

BBa_P0153
type: ComponentDefinition
persistent identity: BBa P0153
display id: BBa_P0153
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PoPS -> cII (p22) [S0167]
creator: Randy Rettberg
created: 2004-04-24T11:00:00Z
modified: 2015-05-08T01:14:10Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Protein generator converting TIPS to the protein cII from p22. Used as the input section for Quad Part Inverters Q01530.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_C0053
component: BBa_B0031
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_C0053
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_B0031
sequence: https://synbiohub.org/public/igem/BBa_P0153_sequence/1

BBa_C0053
type: Component
persistent identity: component944580
display id: component944580
version: 1
top level: BBa_P0153
access: public
definition: c2 P22

BBa_B0031
type: Component
persistent identity: component944568
display id: component944568
version: 1
top level: BBa_P0153
access: public
definition: BBa_B0031

BBa_B0012
type: Component
persistent identity: component944598
display id: component944598
version: 1
top level: BBa_P0153
access: public
definition: BBa_B0012

BBa_B0010
type: Component
persistent identity: component944588
display id: component944588
version: 1
top level: BBa_P0153
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_C0053
type: SequenceAnnotation
persistent identity: annotation944580
display id: annotation944580
version: 1
top level: BBa_P0153
location: https://synbiohub.org/public/igem/BBa_P0153/annotation944580/range944580/1
component: BBa_C0053

https://synbiohub.org/public/igem/BBa_P0153/annotation944580/range944580/1
type: Range
persistent identity: range944580
display id: range944580
version: 1
direction: direction/forward
top level: BBa_P0153
start: 21
end: 707
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation944588
display id: annotation944588
version: 1
top level: BBa_P0153
location: https://synbiohub.org/public/igem/BBa_P0153/annotation944588/range944588/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_P0153/annotation944588/range944588/1
type: Range
persistent identity: range944588
display id: range944588
version: 1
direction: direction/forward
top level: BBa_P0153
start: 741
end: 820
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation944598
display id: annotation944598
version: 1
top level: BBa_P0153
location: https://synbiohub.org/public/igem/BBa_P0153/annotation944598/range944598/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_P0153/annotation944598/range944598/1
type: Range
persistent identity: range944598
display id: range944598
version: 1
direction: direction/forward
top level: BBa_P0153
start: 829
end: 869
orientation: inline

BBa_B0031
type: SequenceAnnotation
persistent identity: annotation944568
display id: annotation944568
version: 1
top level: BBa_P0153
location: https://synbiohub.org/public/igem/BBa_P0153/annotation944568/range944568/1
component: BBa_B0031

https://synbiohub.org/public/igem/BBa_P0153/annotation944568/range944568/1
type: Range
persistent identity: range944568
display id: range944568
version: 1
direction: direction/forward
top level: BBa_P0153
start: 1
end: 14
orientation: inline

BBa_K876013
type: ComponentDefinition
persistent identity: BBa K876013
display id: BBa_K876013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LacI-RFP-ST-pLac-LuxI
creator: Timothy Cogan
created: 2012-09-29T11:00:00Z
modified: 2015-05-08T01:13:38Z
discontinued: false
dominant: false
group u list: _1138_
m user id: 0
owner id: 13836
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: LacI and RFP attached to a constitutive promoter for LuxI
mutable notes: Biobrick placement
mutable provenance: Generated from biobricks
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_C0012
component: BBa_C0061
component: BBa_R0010
component: BBa_K081014
sequence annotation: BBa_C0061
sequence annotation: BBa_R0010
sequence annotation: BBa_K081014
sequence annotation: BBa_C0012
sequence: https://synbiohub.org/public/igem/BBa_K876013_sequence/1

BBa_C0012
type: Component
persistent identity: component2264680
display id: component2264680
version: 1
top level: BBa_K876013
access: public
definition: lacI

BBa_C0061
type: Component
persistent identity: component2264704
display id: component2264704
version: 1
top level: BBa_K876013
access: public
definition: luxI

BBa_R0010
type: Component
persistent identity: component2264696
display id: component2264696
version: 1
top level: BBa_K876013
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_K081014
type: Component
persistent identity: component2264695
display id: component2264695
version: 1
top level: BBa_K876013
access: public
definition: https://synbiohub.org/public/igem/BBa_K081014/1

BBa_C0061
type: SequenceAnnotation
persistent identity: annotation2264704
display id: annotation2264704
version: 1
top level: BBa_K876013
location: https://synbiohub.org/public/igem/BBa_K876013/annotation2264704/range2264704/1
component: BBa_C0061

https://synbiohub.org/public/igem/BBa_K876013/annotation2264704/range2264704/1
type: Range
persistent identity: range2264704
display id: range2264704
version: 1
direction: direction/forward
top level: BBa_K876013
start: 2150
end: 2767
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2264696
display id: annotation2264696
version: 1
top level: BBa_K876013
location: https://synbiohub.org/public/igem/BBa_K876013/annotation2264696/range2264696/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K876013/annotation2264696/range2264696/1
type: Range
persistent identity: range2264696
display id: range2264696
version: 1
direction: direction/forward
top level: BBa_K876013
start: 1944
end: 2143
orientation: inline

BBa_K081014
type: SequenceAnnotation
persistent identity: annotation2264695
display id: annotation2264695
version: 1
top level: BBa_K876013
location: https://synbiohub.org/public/igem/BBa_K876013/annotation2264695/range2264695/1
component: BBa_K081014

https://synbiohub.org/public/igem/BBa_K876013/annotation2264695/range2264695/1
type: Range
persistent identity: range2264695
display id: range2264695
version: 1
direction: direction/reverse
top level: BBa_K876013
start: 1162
end: 1935
orientation: inline

BBa_C0012
type: SequenceAnnotation
persistent identity: annotation2264680
display id: annotation2264680
version: 1
top level: BBa_K876013
location: https://synbiohub.org/public/igem/BBa_K876013/annotation2264680/range2264680/1
component: BBa_C0012

https://synbiohub.org/public/igem/BBa_K876013/annotation2264680/range2264680/1
type: Range
persistent identity: range2264680
display id: range2264680
version: 1
direction: direction/forward
top level: BBa_K876013
start: 1
end: 1128
orientation: inline

cI lam
type: ComponentDefinition
persistent identity: BBa C0051
display id: BBa_C0051
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cI repressor from E. coli phage lambda (+LVA)
creator: Vinay S Mahajan, Brian Chow, Peter Carr, Voichita Marinescu and Alexander D. Wissner-Gross
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Coding region for the cI repressor based on cI repressor from bacteriophage lambda modified with an LVA tail for rapid degradation of the protein. cI repressor binds to the cI regulator (BBa_R0051).
mutable notes: References (unparsed) here: A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000 Octamerization of CI repressor is needed for effective repression of PRM and efficient switching from lysogeny. Ian B. Dodd,1 Alison J. Perkins, Daniel Tsemitsidis, and J. Barry Egan , Genes and Development (Vol 15, No. 22) 3013-3022: 2001 References (unparsed) here: A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000 Octamerization of CI repressor is needed for effective repression of PRM and efficient switching from lysogeny. Ian B. Dodd,1 Alison J. Perkins, Daniel Tsemitsidis, and J. Barry Egan , Genes and Development (Vol 15, No. 22) 3013-3022: 2001 BBa_C0051 cI repressor is based on the cI repressor from the Elowitz's repressilator. It has been modified to include a rapid degradation LAA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.
mutable provenance: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999).
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: LVA
sequence annotation: cI lambda
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0051_sequence/1

LVA
type: SequenceAnnotation
persistent identity: annotation23335
display id: annotation23335
version: 1
top level: cI lam
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_C0051/annotation23335/range23335/1

https://synbiohub.org/public/igem/BBa_C0051/annotation23335/range23335/1
type: Range
persistent identity: range23335
display id: range23335
version: 1
direction: direction/forward
top level: cI lam
start: 712
end: 744
orientation: inline

cI lambda
type: SequenceAnnotation
persistent identity: annotation23334
display id: annotation23334
version: 1
top level: cI lam
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0051/annotation23334/range23334/1

https://synbiohub.org/public/igem/BBa_C0051/annotation23334/range23334/1
type: Range
persistent identity: range23334
display id: range23334
version: 1
direction: direction/forward
top level: cI lam
start: 4
end: 711
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213991
display id: annotation2213991
version: 1
top level: cI lam
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_C0051/annotation2213991/range2213991/1

https://synbiohub.org/public/igem/BBa_C0051/annotation2213991/range2213991/1
type: Range
persistent identity: range2213991
display id: range2213991
version: 1
direction: direction/forward
top level: cI lam
start: 751
end: 775
orientation: inline

araC-CysE
type: ComponentDefinition
persistent identity: BBa K731020
display id: BBa_K731020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Inducible araC-pBAD promoter regulating WT CysE
creator: Jason Fontana
created: 2012-08-20T11:00:00Z
modified: 2015-05-08T01:13:06Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _977_
m user id: 0
owner id: 12113
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: The CysE gene (K731000) is here regulated by the araC-pBAD promoter (K731200), which is inducible by arabinose.
mutable notes: CysE was inserted after araC-pBAD in his vector, cutting the former with X&P and the latter with S&P.
mutable provenance: K731200, K731000
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K731000
component: BBa_K731201
sequence annotation: BBa_K731201
sequence annotation: BBa_K731000
sequence: https://synbiohub.org/public/igem/BBa_K731020_sequence/1

BBa_K731000
type: Component
persistent identity: component2331174
display id: component2331174
version: 1
top level: araC-CysE
access: public
definition: https://synbiohub.org/public/igem/BBa_K731000/1

BBa_K731201
type: Component
persistent identity: component2331169
display id: component2331169
version: 1
top level: araC-CysE
access: public
definition: https://synbiohub.org/public/igem/BBa_K731201/1

BBa_K731201
type: SequenceAnnotation
persistent identity: annotation2331169
display id: annotation2331169
version: 1
top level: araC-CysE
location: https://synbiohub.org/public/igem/BBa_K731020/annotation2331169/range2331169/1
component: BBa_K731201

https://synbiohub.org/public/igem/BBa_K731020/annotation2331169/range2331169/1
type: Range
persistent identity: range2331169
display id: range2331169
version: 1
direction: direction/reverse
top level: araC-CysE
start: 1
end: 1203
orientation: inline

BBa_K731000
type: SequenceAnnotation
persistent identity: annotation2331174
display id: annotation2331174
version: 1
top level: araC-CysE
location: https://synbiohub.org/public/igem/BBa_K731020/annotation2331174/range2331174/1
component: BBa_K731000

https://synbiohub.org/public/igem/BBa_K731020/annotation2331174/range2331174/1
type: Range
persistent identity: range2331174
display id: range2331174
version: 1
direction: direction/reverse
top level: araC-CysE
start: 1212
end: 2050
orientation: inline

BBa_I6108
type: ComponentDefinition
persistent identity: BBa I6108
display id: BBa_I6108
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter O_H Test R0080.E0430
creator: cconboy
created: 2004-05-09T11:00:00Z
modified: 2015-08-31T04:07:44Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 60
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_B0034
component: BBa_E0030
component: BBa_B0012
component: BBa_B0010
component: BBa_R0080
sequence annotation: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_B0010
sequence annotation: BBa_E0030
sequence annotation: BBa_R0080
sequence: https://synbiohub.org/public/igem/BBa_I6108_sequence/1

BBa_B0034
type: Component
persistent identity: component949962
display id: component949962
version: 1
top level: BBa_I6108
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_E0030
type: Component
persistent identity: component949964
display id: component949964
version: 1
top level: BBa_I6108
access: public
definition: https://synbiohub.org/public/igem/BBa_E0030/1

BBa_B0012
type: Component
persistent identity: component949979
display id: component949979
version: 1
top level: BBa_I6108
access: public
definition: BBa_B0012

BBa_B0010
type: Component
persistent identity: component949969
display id: component949969
version: 1
top level: BBa_I6108
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_R0080
type: Component
persistent identity: component949957
display id: component949957
version: 1
top level: BBa_I6108
access: public
definition: AraC

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation949979
display id: annotation949979
version: 1
top level: BBa_I6108
location: https://synbiohub.org/public/igem/BBa_I6108/annotation949979/range949979/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I6108/annotation949979/range949979/1
type: Range
persistent identity: range949979
display id: range949979
version: 1
direction: direction/forward
top level: BBa_I6108
start: 995
end: 1035
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation949962
display id: annotation949962
version: 1
top level: BBa_I6108
location: https://synbiohub.org/public/igem/BBa_I6108/annotation949962/range949962/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I6108/annotation949962/range949962/1
type: Range
persistent identity: range949962
display id: range949962
version: 1
direction: direction/forward
top level: BBa_I6108
start: 158
end: 169
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation949969
display id: annotation949969
version: 1
top level: BBa_I6108
location: https://synbiohub.org/public/igem/BBa_I6108/annotation949969/range949969/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I6108/annotation949969/range949969/1
type: Range
persistent identity: range949969
display id: range949969
version: 1
direction: direction/forward
top level: BBa_I6108
start: 907
end: 986
orientation: inline

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation949964
display id: annotation949964
version: 1
top level: BBa_I6108
location: https://synbiohub.org/public/igem/BBa_I6108/annotation949964/range949964/1
component: BBa_E0030

https://synbiohub.org/public/igem/BBa_I6108/annotation949964/range949964/1
type: Range
persistent identity: range949964
display id: range949964
version: 1
direction: direction/forward
top level: BBa_I6108
start: 176
end: 898
orientation: inline

BBa_R0080
type: SequenceAnnotation
persistent identity: annotation949957
display id: annotation949957
version: 1
top level: BBa_I6108
location: https://synbiohub.org/public/igem/BBa_I6108/annotation949957/range949957/1
component: BBa_R0080

https://synbiohub.org/public/igem/BBa_I6108/annotation949957/range949957/1
type: Range
persistent identity: range949957
display id: range949957
version: 1
direction: direction/forward
top level: BBa_I6108
start: 1
end: 149
orientation: inline

BBa_I732018
type: ComponentDefinition
persistent identity: BBa I732018
display id: BBa_I732018
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: lacZ alpha fragment (RBS+ TERM-)
creator: Zhan Jian
created: 2007-07-13T11:00:00Z
modified: 2015-08-31T04:07:56Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: None
mutable notes: None
mutable provenance: I732006 inserted into pSB1A2-B0034
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_B0034
component: BBa_I732006
sequence annotation: BBa_I732006
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_I732018_sequence/1

BBa_B0034
type: Component
persistent identity: component1938196
display id: component1938196
version: 1
top level: BBa_I732018
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_I732006
type: Component
persistent identity: component1938200
display id: component1938200
version: 1
top level: BBa_I732018
access: public
definition: https://synbiohub.org/public/igem/BBa_I732006/1

BBa_I732006
type: SequenceAnnotation
persistent identity: annotation1938200
display id: annotation1938200
version: 1
top level: BBa_I732018
location: https://synbiohub.org/public/igem/BBa_I732018/annotation1938200/range1938200/1
component: BBa_I732006

https://synbiohub.org/public/igem/BBa_I732018/annotation1938200/range1938200/1
type: Range
persistent identity: range1938200
display id: range1938200
version: 1
direction: direction/reverse
top level: BBa_I732018
start: 19
end: 252
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1938196
display id: annotation1938196
version: 1
top level: BBa_I732018
location: https://synbiohub.org/public/igem/BBa_I732018/annotation1938196/range1938196/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I732018/annotation1938196/range1938196/1
type: Range
persistent identity: range1938196
display id: range1938196
version: 1
direction: direction/reverse
top level: BBa_I732018
start: 1
end: 12
orientation: inline

BBa_K801093
type: ComponentDefinition
persistent identity: BBa K801093
display id: BBa_K801093
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 4-coumarate--coenzyme A ligase (4CL) coding region
creator: Ingmar Polte
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
discontinued: false
dominant: false
group u list: _1057_
m user id: 0
owner id: 13150
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: 4-coumarate--coenzyme A ligase (4CL) coding region from ''Arabidopsis thaliana''.
mutable notes: -
mutable provenance: Plasmid (template for PCR) provided by John A. Morgan, School of Chemical Engineering, Purdue University, West Lafayette, Indiana, USA http://www.ncbi.nlm.nih.gov/nucleotide/609339/ PMID: 15932991
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: 4CL
sequence annotation: Stop codon
sequence annotation: Start codon
sequence: https://synbiohub.org/public/igem/BBa_K801093_sequence/1

4CL
type: SequenceAnnotation
persistent identity: annotation2202143
display id: annotation2202143
version: 1
top level: BBa_K801093
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K801093/annotation2202143/range2202143/1

https://synbiohub.org/public/igem/BBa_K801093/annotation2202143/range2202143/1
type: Range
persistent identity: range2202143
display id: range2202143
version: 1
direction: direction/forward
top level: BBa_K801093
start: 1
end: 1683
orientation: inline

Stop codon
type: SequenceAnnotation
persistent identity: annotation2202141
display id: annotation2202141
version: 1
top level: BBa_K801093
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K801093/annotation2202141/range2202141/1

https://synbiohub.org/public/igem/BBa_K801093/annotation2202141/range2202141/1
type: Range
persistent identity: range2202141
display id: range2202141
version: 1
direction: direction/forward
top level: BBa_K801093
start: 1684
end: 1686
orientation: inline

Start codon
type: SequenceAnnotation
persistent identity: annotation2202140
display id: annotation2202140
version: 1
top level: BBa_K801093
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K801093/annotation2202140/range2202140/1

https://synbiohub.org/public/igem/BBa_K801093/annotation2202140/range2202140/1
type: Range
persistent identity: range2202140
display id: range2202140
version: 1
direction: direction/forward
top level: BBa_K801093
start: 1
end: 3
orientation: inline

RhlR+C4
type: ComponentDefinition
persistent identity: BBa R0071
display id: BBa_R0071
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter (RhlR & C4-HSL regulated)
creator: Ronny Krashinsky
created: 2004-01-24T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Promoter activated by RhlR in concert with C4-HSL. C4-HSL is produced by RhlI, and acts as a quorum-sensing autoinducer. This is the natural sequence taken from Pseudomonas aeruginosa. Crosstalk: this promoter is also activated at a low level by LasR with its associated HSL.
mutable notes: The -10 and -35 sites are labeled as identified in [Pearson97]. The part begins with the RhlR binding site, and (rather arbitrarily) ends with the first transcribed base (+1).
mutable provenance: Pseudomonas aeruginosa rhlAB promoter
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10
sequence annotation: start
sequence annotation: RhlR
sequence annotation: -35
sequence: https://synbiohub.org/public/igem/BBa_R0071_sequence/1

-10
type: SequenceAnnotation
persistent identity: annotation297104
display id: annotation297104
version: 1
top level: RhlR+C4
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0071/annotation297104/range297104/1

https://synbiohub.org/public/igem/BBa_R0071/annotation297104/range297104/1
type: Range
persistent identity: range297104
display id: range297104
version: 1
direction: direction/forward
top level: RhlR+C4
start: 42
end: 47
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation297105
display id: annotation297105
version: 1
top level: RhlR+C4
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_R0071/annotation297105/range297105/1

https://synbiohub.org/public/igem/BBa_R0071/annotation297105/range297105/1
type: Range
persistent identity: range297105
display id: range297105
version: 1
direction: direction/forward
top level: RhlR+C4
start: 53
end: 53
orientation: inline

RhlR
type: SequenceAnnotation
persistent identity: annotation297102
display id: annotation297102
version: 1
top level: RhlR+C4
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0071/annotation297102/range297102/1

https://synbiohub.org/public/igem/BBa_R0071/annotation297102/range297102/1
type: Range
persistent identity: range297102
display id: range297102
version: 1
direction: direction/forward
top level: RhlR+C4
start: 1
end: 20
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation297103
display id: annotation297103
version: 1
top level: RhlR+C4
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0071/annotation297103/range297103/1

https://synbiohub.org/public/igem/BBa_R0071/annotation297103/range297103/1
type: Range
persistent identity: range297103
display id: range297103
version: 1
direction: direction/forward
top level: RhlR+C4
start: 19
end: 24
orientation: inline

BBa_K880005
type: ComponentDefinition
persistent identity: BBa K880005
display id: BBa_K880005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Strong promoter, strong RBS combination for high expression levels of proteins
creator: Josh Atkinson, Mike Ferguson, and Ben Parker
created: 2012-09-28T11:00:00Z
modified: 2015-05-08T01:13:40Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1142_
m user id: 0
owner id: 9403
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -J23100+B0034 -Strong promoter, strong RBS combination for high expression levels of proteins Consensus constitutive promoter and RBS sequence-produces strongest possible expression.
mutable notes: Enter design considerations.
mutable provenance: It is a combination of strong promoter (J23100) and RBS (B0034).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0034
component: BBa_J23100
sequence annotation: BBa_J23100
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K880005_sequence/1

BBa_B0034
type: Component
persistent identity: component2204230
display id: component2204230
version: 1
top level: BBa_K880005
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_J23100
type: Component
persistent identity: component2204228
display id: component2204228
version: 1
top level: BBa_K880005
access: public
definition: https://synbiohub.org/public/igem/BBa_J23100/1

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2204228
display id: annotation2204228
version: 1
top level: BBa_K880005
location: https://synbiohub.org/public/igem/BBa_K880005/annotation2204228/range2204228/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K880005/annotation2204228/range2204228/1
type: Range
persistent identity: range2204228
display id: range2204228
version: 1
direction: direction/reverse
top level: BBa_K880005
start: 1
end: 35
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2204230
display id: annotation2204230
version: 1
top level: BBa_K880005
location: https://synbiohub.org/public/igem/BBa_K880005/annotation2204230/range2204230/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K880005/annotation2204230/range2204230/1
type: Range
persistent identity: range2204230
display id: range2204230
version: 1
direction: direction/reverse
top level: BBa_K880005
start: 44
end: 55
orientation: inline

BBa_I9026
type: ComponentDefinition
persistent identity: BBa I9026
display id: BBa_I9026
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: rhlI translational unit
creator: crackdots
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:08:11Z
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_C0070
component: BBa_B0034
sequence annotation: BBa_C0070
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_I9026_sequence/1

BBa_C0070
type: Component
persistent identity: component939656
display id: component939656
version: 1
top level: BBa_I9026
access: public
definition: https://synbiohub.org/public/igem/BBa_C0070/1

BBa_B0034
type: Component
persistent identity: component939646
display id: component939646
version: 1
top level: BBa_I9026
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_C0070
type: SequenceAnnotation
persistent identity: annotation939656
display id: annotation939656
version: 1
top level: BBa_I9026
location: https://synbiohub.org/public/igem/BBa_I9026/annotation939656/range939656/1
component: BBa_C0070

https://synbiohub.org/public/igem/BBa_I9026/annotation939656/range939656/1
type: Range
persistent identity: range939656
display id: range939656
version: 1
direction: direction/forward
top level: BBa_I9026
start: 19
end: 660
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation939646
display id: annotation939646
version: 1
top level: BBa_I9026
location: https://synbiohub.org/public/igem/BBa_I9026/annotation939646/range939646/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I9026/annotation939646/range939646/1
type: Range
persistent identity: range939646
display id: range939646
version: 1
direction: direction/forward
top level: BBa_I9026
start: 1
end: 12
orientation: inline

BBa_I13600
type: ComponentDefinition
persistent identity: BBa I13600
display id: BBa_I13600
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tet with CFP reporter (without LVA tag)
creator: Christopher Batten, Victoria Chou, Kenneth Nesmith
created: 2004-07-15T11:00:00Z
modified: 2015-08-31T04:07:35Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _6_
m user id: 0
owner id: 101
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_E0020
component: BBa_B0010
component: BBa_R0040
component: BBa_B0034
component: BBa_B0012
sequence annotation: BBa_R0040
sequence annotation: BBa_B0010
sequence annotation: BBa_E0020
sequence annotation: BBa_B0012
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_I13600_sequence/1

BBa_E0020
type: Component
persistent identity: component947194
display id: component947194
version: 1
top level: BBa_I13600
access: public
definition: ecfp

BBa_B0010
type: Component
persistent identity: component947199
display id: component947199
version: 1
top level: BBa_I13600
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_R0040
type: Component
persistent identity: component947184
display id: component947184
version: 1
top level: BBa_I13600
access: public
definition: p(tetR)

BBa_B0034
type: Component
persistent identity: component947192
display id: component947192
version: 1
top level: BBa_I13600
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: Component
persistent identity: component947209
display id: component947209
version: 1
top level: BBa_I13600
access: public
definition: BBa_B0012

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation947184
display id: annotation947184
version: 1
top level: BBa_I13600
location: https://synbiohub.org/public/igem/BBa_I13600/annotation947184/range947184/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_I13600/annotation947184/range947184/1
type: Range
persistent identity: range947184
display id: range947184
version: 1
direction: direction/forward
top level: BBa_I13600
start: 1
end: 54
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation947199
display id: annotation947199
version: 1
top level: BBa_I13600
location: https://synbiohub.org/public/igem/BBa_I13600/annotation947199/range947199/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13600/annotation947199/range947199/1
type: Range
persistent identity: range947199
display id: range947199
version: 1
direction: direction/forward
top level: BBa_I13600
start: 812
end: 891
orientation: inline

BBa_E0020
type: SequenceAnnotation
persistent identity: annotation947194
display id: annotation947194
version: 1
top level: BBa_I13600
location: https://synbiohub.org/public/igem/BBa_I13600/annotation947194/range947194/1
component: BBa_E0020

https://synbiohub.org/public/igem/BBa_I13600/annotation947194/range947194/1
type: Range
persistent identity: range947194
display id: range947194
version: 1
direction: direction/forward
top level: BBa_I13600
start: 81
end: 803
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation947209
display id: annotation947209
version: 1
top level: BBa_I13600
location: https://synbiohub.org/public/igem/BBa_I13600/annotation947209/range947209/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13600/annotation947209/range947209/1
type: Range
persistent identity: range947209
display id: range947209
version: 1
direction: direction/forward
top level: BBa_I13600
start: 900
end: 940
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation947192
display id: annotation947192
version: 1
top level: BBa_I13600
location: https://synbiohub.org/public/igem/BBa_I13600/annotation947192/range947192/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13600/annotation947192/range947192/1
type: Range
persistent identity: range947192
display id: range947192
version: 1
direction: direction/forward
top level: BBa_I13600
start: 63
end: 74
orientation: inline

BBa_J202002
type: ComponentDefinition
persistent identity: BBa J202002
display id: BBa_J202002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PBAD Mutant#3
creator: Jiyeon Park
created: 2012-07-25T11:00:00Z
modified: 2015-08-31T04:08:37Z
discontinued: false
dominant: false
group u list: _910_
m user id: 0
owner id: 11164
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is PBAD (BBa_I13453) mutant. -71 relative to the araC transcriptional start site was changed from A to G. Also, C at -22 was changed to T
mutable notes: In order to regulate transcription from PBAD, araC binds three different sites called araO2, araI1, and araI2. In the absence of L-arabinose, araC binds both araO2 and araI1 and bends DNA preventing transcription from PBAD. In the presence of L-arabinose, however, araC has a conformational change because of L-arabinose and binds araI1 and araI2, enabling transcription from PBAD. Like the wild-type (I13453), this part does not have araO2 site which is essential for araC repressor function. Therefore, araC acts as an activator in the presence of L-arabinose.
mutable provenance: This mutant was created by random mutagenesis.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: araI2
sequence annotation: Transcription Start
sequence annotation: Mutation 1
sequence annotation: Mutation 2
sequence annotation: araI1
sequence: https://synbiohub.org/public/igem/BBa_J202002_sequence/1

araI2
type: SequenceAnnotation
persistent identity: annotation2178422
display id: annotation2178422
version: 1
top level: BBa_J202002
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J202002/annotation2178422/range2178422/1

https://synbiohub.org/public/igem/BBa_J202002/annotation2178422/range2178422/1
type: Range
persistent identity: range2178422
display id: range2178422
version: 1
direction: direction/forward
top level: BBa_J202002
start: 61
end: 77
orientation: inline

Transcription Start
type: SequenceAnnotation
persistent identity: annotation2178423
display id: annotation2178423
version: 1
top level: BBa_J202002
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_J202002/annotation2178423/range2178423/1

https://synbiohub.org/public/igem/BBa_J202002/annotation2178423/range2178423/1
type: Range
persistent identity: range2178423
display id: range2178423
version: 1
direction: direction/forward
top level: BBa_J202002
start: 112
end: 112
orientation: inline

Mutation 1
type: SequenceAnnotation
persistent identity: annotation2178424
display id: annotation2178424
version: 1
top level: BBa_J202002
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_J202002/annotation2178424/range2178424/1

https://synbiohub.org/public/igem/BBa_J202002/annotation2178424/range2178424/1
type: Range
persistent identity: range2178424
display id: range2178424
version: 1
direction: direction/forward
top level: BBa_J202002
start: 41
end: 41
orientation: inline

Mutation 2
type: SequenceAnnotation
persistent identity: annotation2178425
display id: annotation2178425
version: 1
top level: BBa_J202002
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_J202002/annotation2178425/range2178425/1

https://synbiohub.org/public/igem/BBa_J202002/annotation2178425/range2178425/1
type: Range
persistent identity: range2178425
display id: range2178425
version: 1
direction: direction/forward
top level: BBa_J202002
start: 90
end: 90
orientation: inline

araI1
type: SequenceAnnotation
persistent identity: annotation2178421
display id: annotation2178421
version: 1
top level: BBa_J202002
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J202002/annotation2178421/range2178421/1

https://synbiohub.org/public/igem/BBa_J202002/annotation2178421/range2178421/1
type: Range
persistent identity: range2178421
display id: range2178421
version: 1
direction: direction/forward
top level: BBa_J202002
start: 40
end: 56
orientation: inline

BBa_K909008
type: ComponentDefinition
persistent identity: BBa K909008
display id: BBa_K909008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: tetR-DBD-UVR8 fusion protein
creator: Gintautas Vainorius
created: 2012-09-22T11:00:00Z
modified: 2015-05-08T01:13:45Z
discontinued: false
dominant: false
group u list: _1174_
m user id: 0
owner id: 14094
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Tetracycline repressor DNA binding domain fusion with truncated version of UVR8 (tetR-DBD-UVR8). UVR8 is a UV-B sensing receptor in plants necessary for UV-B protection. In dark state UVR8 forms a dimer which is monomerized by the UV-B light (280-315 nm). A truncated version of UVR8 (14-440 amino acids) was fused with tetR DNA binding domain (tetR-DBD), lacking of dimerization domain. Such mutant shows no repression of Ptet promoter, however fusion with dimerizing protein (UVR8) returns tetR-DBD activity. Later UV-B exposure breaks UVR8 dimer and releases tetR-DBD-UVR8 from Ptet, activating transcription. Thus, this tetR-DBD-UVR8 can be used as a one step UV-B on switch.
mutable notes: Contains two illegal PstI sites in UVR8 coding region and BamHI site was used for protein fusion
mutable provenance: tetR-DBD was cloned from E.coli tetracyclin repressor tetR from transposon Tn10 cDNA of UVR8 was provided by Roman Ulm, University of Geneva.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: UVR8
sequence annotation: tetR-DBD
sequence: https://synbiohub.org/public/igem/BBa_K909008_sequence/1

UVR8
type: SequenceAnnotation
persistent identity: annotation2194629
display id: annotation2194629
version: 1
top level: BBa_K909008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K909008/annotation2194629/range2194629/1

https://synbiohub.org/public/igem/BBa_K909008/annotation2194629/range2194629/1
type: Range
persistent identity: range2194629
display id: range2194629
version: 1
direction: direction/forward
top level: BBa_K909008
start: 391
end: 1674
orientation: inline

tetR-DBD
type: SequenceAnnotation
persistent identity: annotation2194628
display id: annotation2194628
version: 1
top level: BBa_K909008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K909008/annotation2194628/range2194628/1

https://synbiohub.org/public/igem/BBa_K909008/annotation2194628/range2194628/1
type: Range
persistent identity: range2194628
display id: range2194628
version: 1
direction: direction/forward
top level: BBa_K909008
start: 1
end: 390
orientation: inline

BBa_I732815
type: ComponentDefinition
persistent identity: BBa I732815
display id: BBa_I732815
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ARL4A0604 (RBS+, TERM-)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:59Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_B0034
component: BBa_I732115
sequence annotation: BBa_B0034
sequence annotation: BBa_I732115
sequence: https://synbiohub.org/public/igem/BBa_I732815_sequence/1

BBa_B0034
type: Component
persistent identity: component1946207
display id: component1946207
version: 1
top level: BBa_I732815
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_I732115
type: Component
persistent identity: component1946208
display id: component1946208
version: 1
top level: BBa_I732815
access: public
definition: https://synbiohub.org/public/igem/BBa_I732115/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1946207
display id: annotation1946207
version: 1
top level: BBa_I732815
location: https://synbiohub.org/public/igem/BBa_I732815/annotation1946207/range1946207/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I732815/annotation1946207/range1946207/1
type: Range
persistent identity: range1946207
display id: range1946207
version: 1
direction: direction/reverse
top level: BBa_I732815
start: 1
end: 12
orientation: inline

BBa_I732115
type: SequenceAnnotation
persistent identity: annotation1946208
display id: annotation1946208
version: 1
top level: BBa_I732815
location: https://synbiohub.org/public/igem/BBa_I732815/annotation1946208/range1946208/1
component: BBa_I732115

https://synbiohub.org/public/igem/BBa_I732815/annotation1946208/range1946208/1
type: Range
persistent identity: range1946208
display id: range1946208
version: 1
direction: direction/reverse
top level: BBa_I732815
start: 19
end: 1104
orientation: inline

PT7-E
type: ComponentDefinition
persistent identity: BBa I716107
display id: BBa_I716107
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 Promoter (Mutant E)
creator: David Tulga
created: 2007-06-08T11:00:00Z
modified: 2015-08-31T04:07:51Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _110_
m user id: 0
owner id: 1694
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is the T7 Promoter which is activated when the T7 RNA Polymerase is produced. It provides very good PoPS (Polymerases Per Second) output, as well as it is highly orthogonal to E. Coli's natural transcription system. This one of the moderately active (Mutant E) variations we are using.
mutable notes: {{BerkiGEM2007-Biobrick2.0Intro}}
mutable provenance: This part was generated synthetically.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/T7
role: SO:0001207
sequence annotation: T7 Promoter E
sequence: https://synbiohub.org/public/igem/BBa_I716107_sequence/1

T7 Promoter E
type: SequenceAnnotation
persistent identity: annotation1954611
display id: annotation1954611
version: 1
top level: PT7-E
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I716107/annotation1954611/range1954611/1

https://synbiohub.org/public/igem/BBa_I716107/annotation1954611/range1954611/1
type: Range
persistent identity: range1954611
display id: range1954611
version: 1
direction: direction/forward
top level: PT7-E
start: 1
end: 29
orientation: inline

BBa_I6030
type: ComponentDefinition
persistent identity: BBa I6030
display id: BBa_I6030
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter O_H Test R0010.E0430
creator: Caitlin Conboy and Jennifer Braff
created: 2004-03-16T12:00:00Z
modified: 2015-08-31T04:07:43Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_B0010
component: BBa_R0010
component: BBa_B0034
component: BBa_B0012
component: BBa_E0030
sequence annotation: BBa_B0034
sequence annotation: BBa_B0012
sequence annotation: BBa_E0030
sequence annotation: BBa_R0010
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_I6030_sequence/1

BBa_B0010
type: Component
persistent identity: component948292
display id: component948292
version: 1
top level: BBa_I6030
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_R0010
type: Component
persistent identity: component948275
display id: component948275
version: 1
top level: BBa_I6030
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_B0034
type: Component
persistent identity: component948285
display id: component948285
version: 1
top level: BBa_I6030
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: Component
persistent identity: component948302
display id: component948302
version: 1
top level: BBa_I6030
access: public
definition: BBa_B0012

BBa_E0030
type: Component
persistent identity: component948287
display id: component948287
version: 1
top level: BBa_I6030
access: public
definition: https://synbiohub.org/public/igem/BBa_E0030/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation948285
display id: annotation948285
version: 1
top level: BBa_I6030
location: https://synbiohub.org/public/igem/BBa_I6030/annotation948285/range948285/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I6030/annotation948285/range948285/1
type: Range
persistent identity: range948285
display id: range948285
version: 1
direction: direction/forward
top level: BBa_I6030
start: 209
end: 220
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation948302
display id: annotation948302
version: 1
top level: BBa_I6030
location: https://synbiohub.org/public/igem/BBa_I6030/annotation948302/range948302/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I6030/annotation948302/range948302/1
type: Range
persistent identity: range948302
display id: range948302
version: 1
direction: direction/forward
top level: BBa_I6030
start: 1046
end: 1086
orientation: inline

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation948287
display id: annotation948287
version: 1
top level: BBa_I6030
location: https://synbiohub.org/public/igem/BBa_I6030/annotation948287/range948287/1
component: BBa_E0030

https://synbiohub.org/public/igem/BBa_I6030/annotation948287/range948287/1
type: Range
persistent identity: range948287
display id: range948287
version: 1
direction: direction/forward
top level: BBa_I6030
start: 227
end: 949
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation948275
display id: annotation948275
version: 1
top level: BBa_I6030
location: https://synbiohub.org/public/igem/BBa_I6030/annotation948275/range948275/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_I6030/annotation948275/range948275/1
type: Range
persistent identity: range948275
display id: range948275
version: 1
direction: direction/forward
top level: BBa_I6030
start: 1
end: 200
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation948292
display id: annotation948292
version: 1
top level: BBa_I6030
location: https://synbiohub.org/public/igem/BBa_I6030/annotation948292/range948292/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I6030/annotation948292/range948292/1
type: Range
persistent identity: range948292
display id: range948292
version: 1
direction: direction/forward
top level: BBa_I6030
start: 958
end: 1037
orientation: inline

BBa_K763005
type: ComponentDefinition
persistent identity: BBa K763005
display id: BBa_K763005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pADH2 + Gene encoding YAP1 protein
creator: Luisa Mar??a Mart??nez S??nchez
created: 2012-09-16T11:00:00Z
modified: 2015-05-08T01:13:13Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1014_
m user id: 0
owner id: 11712
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: There are two transcriptional factors which regulates the ADH2 promoter: ADR1 a carbon source-responsibe zinc-finger2 and CAT83. In the absence of glucose both cis-acting sites in the promoter are bound cooperatively by the transcriptional activators. ADR1 binds to the UAS1 site while CAT8 binds to the UAS2/CSRE site and regulates positively the transcription of YAP1 protein by the activation of the ADH2 promoter. The presence of glucose downregulates the levels of the transcription factors (ADR1 and CAT8) which results in the depletion of the production of YAP1 protein by the repression of the ADH2 promoter.
mutable notes: No design considerations needed.
mutable provenance: The sequences of the promoter and the gene which encodes the YAP1 protein comes from yeast Sacharomyces cerevisiae genome.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K763005_sequence/1

BBa_I13602
type: ComponentDefinition
persistent identity: BBa I13602
display id: BBa_I13602
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tet operator with CFP reporter (with LVA tag) [R/Tc+]
creator: Christopher Batten, Victoria Chou, Kenneth Nesmith
created: 2004-07-15T11:00:00Z
modified: 2015-08-31T04:07:36Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _6_
m user id: 0
owner id: 101
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0012
component: BBa_R0040
component: BBa_B0034
component: BBa_B0010
component: BBa_E0022
sequence annotation: BBa_E0022
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_R0040
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_I13602_sequence/1

BBa_B0012
type: Component
persistent identity: component947292
display id: component947292
version: 1
top level: BBa_I13602
access: public
definition: BBa_B0012

BBa_R0040
type: Component
persistent identity: component947254
display id: component947254
version: 1
top level: BBa_I13602
access: public
definition: p(tetR)

BBa_B0034
type: Component
persistent identity: component947262
display id: component947262
version: 1
top level: BBa_I13602
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component947282
display id: component947282
version: 1
top level: BBa_I13602
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_E0022
type: Component
persistent identity: component947274
display id: component947274
version: 1
top level: BBa_I13602
access: public
definition: ECFP

BBa_E0022
type: SequenceAnnotation
persistent identity: annotation947274
display id: annotation947274
version: 1
top level: BBa_I13602
location: https://synbiohub.org/public/igem/BBa_I13602/annotation947274/range947274/1
component: BBa_E0022

https://synbiohub.org/public/igem/BBa_I13602/annotation947274/range947274/1
type: Range
persistent identity: range947274
display id: range947274
version: 1
direction: direction/forward
top level: BBa_I13602
start: 81
end: 842
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation947292
display id: annotation947292
version: 1
top level: BBa_I13602
location: https://synbiohub.org/public/igem/BBa_I13602/annotation947292/range947292/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13602/annotation947292/range947292/1
type: Range
persistent identity: range947292
display id: range947292
version: 1
direction: direction/forward
top level: BBa_I13602
start: 939
end: 979
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation947282
display id: annotation947282
version: 1
top level: BBa_I13602
location: https://synbiohub.org/public/igem/BBa_I13602/annotation947282/range947282/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13602/annotation947282/range947282/1
type: Range
persistent identity: range947282
display id: range947282
version: 1
direction: direction/forward
top level: BBa_I13602
start: 851
end: 930
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation947254
display id: annotation947254
version: 1
top level: BBa_I13602
location: https://synbiohub.org/public/igem/BBa_I13602/annotation947254/range947254/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_I13602/annotation947254/range947254/1
type: Range
persistent identity: range947254
display id: range947254
version: 1
direction: direction/forward
top level: BBa_I13602
start: 1
end: 54
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation947262
display id: annotation947262
version: 1
top level: BBa_I13602
location: https://synbiohub.org/public/igem/BBa_I13602/annotation947262/range947262/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13602/annotation947262/range947262/1
type: Range
persistent identity: range947262
display id: range947262
version: 1
direction: direction/forward
top level: BBa_I13602
start: 63
end: 74
orientation: inline

tctA_505aa
type: ComponentDefinition
persistent identity: BBa K808004
display id: BBa_K808004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: tctA_505: large subunit A2 of the tripartite tricarboxylate transporter family
creator: Valentina Herbring, Sebastian Palluk, Andreas Schmidt
created: 2012-09-04T11:00:00Z
modified: 2015-05-08T01:13:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1065_
m user id: 0
owner id: 12829
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: tctA_505: large subunit A2 of the tripartite tricarboxylate transporter family
mutable notes: deletion of PstI restriction site
mutable provenance: Comamonas testosteroni KF1
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K808004_sequence/1

ECFP
type: ComponentDefinition
persistent identity: BBa E0024
display id: BBa_E0024
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: enhanced cyan fluorescent protein derived from A. victoria GFP
creator: jcbraff
created: 2004-06-06T11:00:00Z
modified: 2015-08-31T04:07:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 61
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_E0024_sequence/1

BBa_K917014
type: ComponentDefinition
persistent identity: BBa K917014
display id: BBa_K917014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pLac promoter plus S. oneidensis tetraheme cytochrome c cymA
creator: Jakub Krakowiak
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:46Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1182_
m user id: 0
owner id: 13804
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This part consists of the standard registry lac promoter (BBa_J33207) S. oneidensis tetraheme cytochrome c cymA (K917009).
mutable notes: no special notes
mutable provenance: pLac gene comes from E. coli BL21 genomic DNA cymA was cloned from S. oneidensis MR-1 genomic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_J33207
component: BBa_K917009
sequence annotation: BBa_J33207
sequence annotation: BBa_K917009
sequence: https://synbiohub.org/public/igem/BBa_K917014_sequence/1

BBa_J33207
type: Component
persistent identity: component2196015
display id: component2196015
version: 1
top level: BBa_K917014
access: public
definition: https://synbiohub.org/public/igem/BBa_J33207/1

BBa_K917009
type: Component
persistent identity: component2196019
display id: component2196019
version: 1
top level: BBa_K917014
access: public
definition: https://synbiohub.org/public/igem/BBa_K917009/1

BBa_J33207
type: SequenceAnnotation
persistent identity: annotation2196015
display id: annotation2196015
version: 1
top level: BBa_K917014
location: https://synbiohub.org/public/igem/BBa_K917014/annotation2196015/range2196015/1
component: BBa_J33207

https://synbiohub.org/public/igem/BBa_K917014/annotation2196015/range2196015/1
type: Range
persistent identity: range2196015
display id: range2196015
version: 1
direction: direction/reverse
top level: BBa_K917014
start: 1
end: 600
orientation: inline

BBa_K917009
type: SequenceAnnotation
persistent identity: annotation2196019
display id: annotation2196019
version: 1
top level: BBa_K917014
location: https://synbiohub.org/public/igem/BBa_K917014/annotation2196019/range2196019/1
component: BBa_K917009

https://synbiohub.org/public/igem/BBa_K917014/annotation2196019/range2196019/1
type: Range
persistent identity: range2196019
display id: range2196019
version: 1
direction: direction/reverse
top level: BBa_K917014
start: 609
end: 1207
orientation: inline

BBa_K741020
type: ComponentDefinition
persistent identity: BBa K741020
display id: BBa_K741020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: plac-RBS-cI-T-pCon(0.856)-RBS-crogfp-T
creator: Wuyang Chen
created: 2012-09-15T11:00:00Z
modified: 2015-05-08T01:13:09Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _990_
m user id: 0
owner id: 11656
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: We make this part in the middle of the progress of making the part plac-RBS-cI-T-pCon(0.856)-RBS-crogfp-T-pRM-anticro-T.
mutable notes: TBD
mutable provenance: BBa_K741001 BBa_K741009
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K741009
component: BBa_K741001
sequence annotation: BBa_K741001
sequence annotation: BBa_K741009
sequence: https://synbiohub.org/public/igem/BBa_K741020_sequence/1

BBa_K741009
type: Component
persistent identity: component2230908
display id: component2230908
version: 1
top level: BBa_K741020
access: public
definition: https://synbiohub.org/public/igem/BBa_K741009/1

BBa_K741001
type: Component
persistent identity: component2230895
display id: component2230895
version: 1
top level: BBa_K741020
access: public
definition: https://synbiohub.org/public/igem/BBa_K741001/1

BBa_K741001
type: SequenceAnnotation
persistent identity: annotation2230895
display id: annotation2230895
version: 1
top level: BBa_K741020
location: https://synbiohub.org/public/igem/BBa_K741020/annotation2230895/range2230895/1
component: BBa_K741001

https://synbiohub.org/public/igem/BBa_K741020/annotation2230895/range2230895/1
type: Range
persistent identity: range2230895
display id: range2230895
version: 1
direction: direction/reverse
top level: BBa_K741020
start: 1
end: 1138
orientation: inline

BBa_K741009
type: SequenceAnnotation
persistent identity: annotation2230908
display id: annotation2230908
version: 1
top level: BBa_K741020
location: https://synbiohub.org/public/igem/BBa_K741020/annotation2230908/range2230908/1
component: BBa_K741009

https://synbiohub.org/public/igem/BBa_K741020/annotation2230908/range2230908/1
type: Range
persistent identity: range2230908
display id: range2230908
version: 1
direction: direction/reverse
top level: BBa_K741020
start: 1147
end: 2277
orientation: inline

LRLa
type: ComponentDefinition
persistent identity: BBa I732101
display id: BBa_I732101
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LRLa
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:57Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_I732101_sequence/1

BBa_I6056
type: ComponentDefinition
persistent identity: BBa I6056
display id: BBa_I6056
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter O_H Test R1062.E0430
creator: Caitlin Conboy and Jennifer Braff
created: 2004-03-16T12:00:00Z
modified: 2015-08-31T04:07:43Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_B0010
component: BBa_B0034
component: BBa_R1062
component: BBa_E0030
component: BBa_B0012
sequence annotation: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_R1062
sequence annotation: BBa_E0030
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_I6056_sequence/1

BBa_B0010
type: Component
persistent identity: component948814
display id: component948814
version: 1
top level: BBa_I6056
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component948807
display id: component948807
version: 1
top level: BBa_I6056
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R1062
type: Component
persistent identity: component948799
display id: component948799
version: 1
top level: BBa_I6056
access: public
definition: lux pR

BBa_E0030
type: Component
persistent identity: component948809
display id: component948809
version: 1
top level: BBa_I6056
access: public
definition: https://synbiohub.org/public/igem/BBa_E0030/1

BBa_B0012
type: Component
persistent identity: component948824
display id: component948824
version: 1
top level: BBa_I6056
access: public
definition: BBa_B0012

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation948824
display id: annotation948824
version: 1
top level: BBa_I6056
location: https://synbiohub.org/public/igem/BBa_I6056/annotation948824/range948824/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I6056/annotation948824/range948824/1
type: Range
persistent identity: range948824
display id: range948824
version: 1
direction: direction/forward
top level: BBa_I6056
start: 902
end: 942
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation948807
display id: annotation948807
version: 1
top level: BBa_I6056
location: https://synbiohub.org/public/igem/BBa_I6056/annotation948807/range948807/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I6056/annotation948807/range948807/1
type: Range
persistent identity: range948807
display id: range948807
version: 1
direction: direction/forward
top level: BBa_I6056
start: 65
end: 76
orientation: inline

BBa_R1062
type: SequenceAnnotation
persistent identity: annotation948799
display id: annotation948799
version: 1
top level: BBa_I6056
location: https://synbiohub.org/public/igem/BBa_I6056/annotation948799/range948799/1
component: BBa_R1062

https://synbiohub.org/public/igem/BBa_I6056/annotation948799/range948799/1
type: Range
persistent identity: range948799
display id: range948799
version: 1
direction: direction/forward
top level: BBa_I6056
start: 1
end: 56
orientation: inline

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation948809
display id: annotation948809
version: 1
top level: BBa_I6056
location: https://synbiohub.org/public/igem/BBa_I6056/annotation948809/range948809/1
component: BBa_E0030

https://synbiohub.org/public/igem/BBa_I6056/annotation948809/range948809/1
type: Range
persistent identity: range948809
display id: range948809
version: 1
direction: direction/forward
top level: BBa_I6056
start: 83
end: 805
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation948814
display id: annotation948814
version: 1
top level: BBa_I6056
location: https://synbiohub.org/public/igem/BBa_I6056/annotation948814/range948814/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I6056/annotation948814/range948814/1
type: Range
persistent identity: range948814
display id: range948814
version: 1
direction: direction/forward
top level: BBa_I6056
start: 814
end: 893
orientation: inline

cI lam
type: ComponentDefinition
persistent identity: BBa R1051
display id: BBa_R1051
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter, Standard (lambda cI regulated)
creator: Vinay S Mahajan, Brian Chow, Peter Carr, Voichita Marinescu and Alexander D. Wissner-Gross Drew Endy
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: [Note: This is the same part as R0051 except that the -10 and -35 sites and spacing have been changed to comply with BBa_S0001]. The cI regulated promoter is based on the pR promtoer from bacteriohage lambda. The promoter has two two DNA binding sites for lambda cI repressor <bb_part>BBa_C0051</bb_part>. cI binding results in repression of transcription. The specific sequence used here is based on the cI repressible promoter used in the Elowitz repressilator (and references therein).
mutable notes: In order to address concerns about the promoter transcribing in the reverse direction, we have removed the -35 and -10 signals responsible for the promoter activity in the reverse direction. (More details needed here! DE, 2/24/03) Modified to comply with BBa_S0001: TTGACA-17N-GATACT Incompatible with host expressing cI repressor.
mutable provenance: A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: OR2
sequence annotation: OR1
sequence annotation: BBa_R1051
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R1051_sequence/1

OR2
type: SequenceAnnotation
persistent identity: annotation2077
display id: annotation2077
version: 1
top level: cI lam
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R1051/annotation2077/range2077/1

https://synbiohub.org/public/igem/BBa_R1051/annotation2077/range2077/1
type: Range
persistent identity: range2077
display id: range2077
version: 1
direction: direction/forward
top level: cI lam
start: 1
end: 17
orientation: inline

OR1
type: SequenceAnnotation
persistent identity: annotation2076
display id: annotation2076
version: 1
top level: cI lam
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R1051/annotation2076/range2076/1

https://synbiohub.org/public/igem/BBa_R1051/annotation2076/range2076/1
type: Range
persistent identity: range2076
display id: range2076
version: 1
direction: direction/forward
top level: cI lam
start: 25
end: 41
orientation: inline

BBa_R1051
type: SequenceAnnotation
persistent identity: annotation7074
display id: annotation7074
version: 1
top level: cI lam
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_R1051/annotation7074/range7074/1

https://synbiohub.org/public/igem/BBa_R1051/annotation7074/range7074/1
type: Range
persistent identity: range7074
display id: range7074
version: 1
direction: direction/forward
top level: cI lam
start: 1
end: 49
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2075
display id: annotation2075
version: 1
top level: cI lam
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R1051/annotation2075/range2075/1

https://synbiohub.org/public/igem/BBa_R1051/annotation2075/range2075/1
type: Range
persistent identity: range2075
display id: range2075
version: 1
direction: direction/forward
top level: cI lam
start: 15
end: 20
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2078
display id: annotation2078
version: 1
top level: cI lam
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R1051/annotation2078/range2078/1

https://synbiohub.org/public/igem/BBa_R1051/annotation2078/range2078/1
type: Range
persistent identity: range2078
display id: range2078
version: 1
direction: direction/forward
top level: cI lam
start: 38
end: 43
orientation: inline

BBa_S03877
type: ComponentDefinition
persistent identity: BBa S03877
display id: BBa_S03877
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0034:I15008
creator: Kathy Lam
created: 2007-10-25T11:00:00Z
modified: 2015-05-08T01:14:31Z
discontinued: false
dominant: false
group u list: _82_
m user id: 0
owner id: 927
owning group id: 82
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_B0034
component: BBa_I15008
sequence annotation: BBa_B0034
sequence annotation: BBa_I15008
sequence: https://synbiohub.org/public/igem/BBa_S03877_sequence/1

BBa_B0034
type: Component
persistent identity: component2243951
display id: component2243951
version: 1
top level: BBa_S03877
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_I15008
type: Component
persistent identity: component2243953
display id: component2243953
version: 1
top level: BBa_S03877
access: public
definition: https://synbiohub.org/public/igem/BBa_I15008/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2243951
display id: annotation2243951
version: 1
top level: BBa_S03877
location: https://synbiohub.org/public/igem/BBa_S03877/annotation2243951/range2243951/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_S03877/annotation2243951/range2243951/1
type: Range
persistent identity: range2243951
display id: range2243951
version: 1
direction: direction/reverse
top level: BBa_S03877
start: 1
end: 12
orientation: inline

BBa_I15008
type: SequenceAnnotation
persistent identity: annotation2243953
display id: annotation2243953
version: 1
top level: BBa_S03877
location: https://synbiohub.org/public/igem/BBa_S03877/annotation2243953/range2243953/1
component: BBa_I15008

https://synbiohub.org/public/igem/BBa_S03877/annotation2243953/range2243953/1
type: Range
persistent identity: range2243953
display id: range2243953
version: 1
direction: direction/reverse
top level: BBa_S03877
start: 19
end: 769
orientation: inline

luxr
type: ComponentDefinition
persistent identity: BBa C0062
display id: BBa_C0062
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: luxR repressor/activator, (no LVA?)
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: In complex with HSL, LuxR binds to the Lux promoter, activating transcription from Pr <bb_part>BBa_R0062</bb_part>, and repressing transcription from Pl <bb_part>BBa_R0063</bb_part>. The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the Lux activator, LuxR complexed to HSL. Two molecules of LuxR protein form a complex with two molecules the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
mutable notes: 2 silent point mutants were introduced in the coding sequence to remove internal XbaI and PstI sites. Mutation sites were chosen to replace codons commonly used in E. coli with codons used at a similar frequency.
mutable provenance: V. fischeri AF170104
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: BBa_C0062
sequence annotation: luxR
sequence annotation: T
sequence annotation: prefix
sequence annotation: Help:Barcodes
sequence annotation: A
sequence: https://synbiohub.org/public/igem/BBa_C0062_sequence/1

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation7039
display id: annotation7039
version: 1
top level: luxr
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_C0062/annotation7039/range7039/1

https://synbiohub.org/public/igem/BBa_C0062/annotation7039/range7039/1
type: Range
persistent identity: range7039
display id: range7039
version: 1
direction: direction/forward
top level: luxr
start: 1
end: 756
orientation: inline

luxR
type: SequenceAnnotation
persistent identity: annotation1766
display id: annotation1766
version: 1
top level: luxr
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1766/range1766/1

https://synbiohub.org/public/igem/BBa_C0062/annotation1766/range1766/1
type: Range
persistent identity: range1766
display id: range1766
version: 1
direction: direction/forward
top level: luxr
start: 1
end: 750
orientation: inline

T
type: SequenceAnnotation
persistent identity: annotation1764
display id: annotation1764
version: 1
top level: luxr
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1764/range1764/1

https://synbiohub.org/public/igem/BBa_C0062/annotation1764/range1764/1
type: Range
persistent identity: range1764
display id: range1764
version: 1
direction: direction/forward
top level: luxr
start: 174
end: 174
orientation: inline

prefix
type: SequenceAnnotation
persistent identity: annotation1762
display id: annotation1762
version: 1
top level: luxr
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1762/range1762/1

https://synbiohub.org/public/igem/BBa_C0062/annotation1762/range1762/1
type: Range
persistent identity: range1762
display id: range1762
version: 1
direction: direction/forward
top level: luxr
start: 1
end: 2
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213986
display id: annotation2213986
version: 1
top level: luxr
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_C0062/annotation2213986/range2213986/1

https://synbiohub.org/public/igem/BBa_C0062/annotation2213986/range2213986/1
type: Range
persistent identity: range2213986
display id: range2213986
version: 1
direction: direction/forward
top level: luxr
start: 757
end: 781
orientation: inline

A
type: SequenceAnnotation
persistent identity: annotation1765
display id: annotation1765
version: 1
top level: luxr
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1765/range1765/1

https://synbiohub.org/public/igem/BBa_C0062/annotation1765/range1765/1
type: Range
persistent identity: range1765
display id: range1765
version: 1
direction: direction/forward
top level: luxr
start: 492
end: 492
orientation: inline

Bxb1_Int
type: ComponentDefinition
persistent identity: BBa K907000
display id: BBa_K907000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Mycobacterium Phage Bxb1 gp35, DNA integrase
creator: Dong-hui Choe, Soo-in Lee
created: 2012-09-19T11:00:00Z
modified: 2015-05-08T01:13:44Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1172_
m user id: 0
owner id: 12628
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This part is the protein coding sequence which encodes DNA integrase of Mycobacterium phage Bxb1. The Bxb1 integrase is a DNA recombinase, more precisely a member of serine integrase family. It recognizes specific sequences, called attB and attP, and then integrate, invert, or excise depend on orientations of recognition sequences. We used this integrase to invert specific sequence contained in plasmid. This protein is well expressed in E.coli(strain MG1655). When it inverts DNA, the attB and attP sequences are changed into attL and attR, as common DNA recombinases do. Another protein called Bxb1 excisionase interacts with integrase and that complex flips back inverted DNA into original sequence regenerating attB and attP sequences.
mutable notes: Internal restriction endonuclease site is changed(two PstI sites, one XbaI sites) without changing amino acid sequence.
mutable provenance: This part is derived by Mycobacterium phage Bxb1. And synthesized by Bioneer. http://www.ncbi.nlm.nih.gov/protein/NP_075302.1
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Mycobacterium phage Bxb1 integrase CDS
sequence: https://synbiohub.org/public/igem/BBa_K907000_sequence/1

Mycobacterium phage Bxb1 integrase CDS
type: SequenceAnnotation
persistent identity: annotation2187533
display id: annotation2187533
version: 1
top level: Bxb1_Int
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K907000/annotation2187533/range2187533/1

https://synbiohub.org/public/igem/BBa_K907000/annotation2187533/range2187533/1
type: Range
persistent identity: range2187533
display id: range2187533
version: 1
direction: direction/forward
top level: Bxb1_Int
start: 1
end: 1503
orientation: inline

BBa_I714031
type: ComponentDefinition
persistent identity: BBa I714031
display id: BBa_I714031
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: OriT-R (Origin of Transfer for the R751-plasmid nic region)
creator: Mingzhi Qu
created: 2007-10-20T11:00:00Z
modified: 2015-08-31T04:07:47Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _142_
m user id: 0
owner id: 2248
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: OriT-R751 (Origin of Transfer for the R751-type conjugative plasmid) is the nic region, where the relaxosome nicks the plasmid and conjugative transfer by R751 plasmid machinery begins via rolling circle replication.
mutable provenance: Enterobacter aerogenes plasmid R751 NCBI:NC_001735
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Conjugation
role: SO:0000724
sequence: https://synbiohub.org/public/igem/BBa_I714031_sequence/1

BBa_K936000
type: ComponentDefinition
persistent identity: BBa K936000
display id: BBa_K936000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LC Cutinase
creator: Nicholas Csicsery
created: 2012-07-16T11:00:00Z
modified: 2015-05-08T01:13:47Z
discontinued: false
dominant: false
group u list: _1201_
m user id: 0
owner id: 10185
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is the LC Cutinase gene used to break PET into terephthalic acid and ethylene glycol.
mutable notes: Eliminated BioBrick restriction sites and added a TAA double stop.
mutable provenance: Modified from the paper Isolation of a Novel Cutinase Homolog with Polyethylene Terephthalate-Degrading Activity from Leaf-Branch Compost by Using a Metagenomic Approach by Sulaiman et al.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K936000_sequence/1

BBa_J202013
type: ComponentDefinition
persistent identity: BBa J202013
display id: BBa_J202013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PBAD Mutant#14
creator: Jiyeon Park
created: 2012-07-25T11:00:00Z
modified: 2015-08-31T04:08:37Z
discontinued: false
dominant: false
group u list: _910_
m user id: 0
owner id: 11164
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is PBAD (BBa_I13453) mutant. -69 relative to the araC transcriptional start site was changed from C to A.
mutable notes: In order to regulate transcription from PBAD, araC binds three different sites called araO2, araI1, and araI2. In the absence of L-arabinose, araC binds both araO2 and araI1 and bends DNA preventing transcription from PBAD. In the presence of L-arabinose, however, araC has a conformational change because of L-arabinose and binds araI1 and araI2, enabling transcription from PBAD. Like the wild-type (I13453), this part does not have araO2 site which is essential for araC repressor function. Therefore, araC acts as an activator in the presence of L-arabinose.
mutable provenance: This mutant was created by site-directed mutagenesis.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Mutation
sequence annotation: Transcription Start
sequence annotation: araI2
sequence annotation: araI1
sequence: https://synbiohub.org/public/igem/BBa_J202013_sequence/1

Mutation
type: SequenceAnnotation
persistent identity: annotation2178469
display id: annotation2178469
version: 1
top level: BBa_J202013
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_J202013/annotation2178469/range2178469/1

https://synbiohub.org/public/igem/BBa_J202013/annotation2178469/range2178469/1
type: Range
persistent identity: range2178469
display id: range2178469
version: 1
direction: direction/forward
top level: BBa_J202013
start: 43
end: 43
orientation: inline

Transcription Start
type: SequenceAnnotation
persistent identity: annotation2178470
display id: annotation2178470
version: 1
top level: BBa_J202013
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_J202013/annotation2178470/range2178470/1

https://synbiohub.org/public/igem/BBa_J202013/annotation2178470/range2178470/1
type: Range
persistent identity: range2178470
display id: range2178470
version: 1
direction: direction/forward
top level: BBa_J202013
start: 112
end: 112
orientation: inline

araI2
type: SequenceAnnotation
persistent identity: annotation2178472
display id: annotation2178472
version: 1
top level: BBa_J202013
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J202013/annotation2178472/range2178472/1

https://synbiohub.org/public/igem/BBa_J202013/annotation2178472/range2178472/1
type: Range
persistent identity: range2178472
display id: range2178472
version: 1
direction: direction/forward
top level: BBa_J202013
start: 61
end: 77
orientation: inline

araI1
type: SequenceAnnotation
persistent identity: annotation2178471
display id: annotation2178471
version: 1
top level: BBa_J202013
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J202013/annotation2178471/range2178471/1

https://synbiohub.org/public/igem/BBa_J202013/annotation2178471/range2178471/1
type: Range
persistent identity: range2178471
display id: range2178471
version: 1
direction: direction/forward
top level: BBa_J202013
start: 40
end: 56
orientation: inline

lux pR
type: ComponentDefinition
persistent identity: BBa R0062
display id: BBa_R0062
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter (luxR & HSL regulated -- lux pR)
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Promoter activated by LuxR in concert with HSL The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the LuxR activator protein complexed with the autoinducer, 3-oxo-hexanoyl-HSL. Two molecules of LuxR protein form a complex with two molecules of the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
mutable notes: This promoter is based on the Vibrio fischeri quorum sensing gene promoters. Two genes LuxI and LuxR and transcribed in opposite directions as shown below. The original sequence from which the parts <bb_part>BBa_R0062</bb_part> and <bb_part>BBa_R0063</bb_part> were derived is shown in the picture below. <img src="<bb_file>Image1.gif</bb_file>" width="614" height="362">
mutable provenance: V. fischeri
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: start
sequence annotation: -35
sequence annotation: -10
sequence annotation: BBa_R0062
sequence annotation: LuxR/HSL
sequence: https://synbiohub.org/public/igem/BBa_R0062_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation2048
display id: annotation2048
version: 1
top level: lux pR
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_R0062/annotation2048/range2048/1

https://synbiohub.org/public/igem/BBa_R0062/annotation2048/range2048/1
type: Range
persistent identity: range2048
display id: range2048
version: 1
direction: direction/forward
top level: lux pR
start: 53
end: 53
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2046
display id: annotation2046
version: 1
top level: lux pR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0062/annotation2046/range2046/1

https://synbiohub.org/public/igem/BBa_R0062/annotation2046/range2046/1
type: Range
persistent identity: range2046
display id: range2046
version: 1
direction: direction/forward
top level: lux pR
start: 20
end: 25
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2047
display id: annotation2047
version: 1
top level: lux pR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0062/annotation2047/range2047/1

https://synbiohub.org/public/igem/BBa_R0062/annotation2047/range2047/1
type: Range
persistent identity: range2047
display id: range2047
version: 1
direction: direction/forward
top level: lux pR
start: 42
end: 47
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation7070
display id: annotation7070
version: 1
top level: lux pR
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_R0062/annotation7070/range7070/1

https://synbiohub.org/public/igem/BBa_R0062/annotation7070/range7070/1
type: Range
persistent identity: range7070
display id: range7070
version: 1
direction: direction/forward
top level: lux pR
start: 1
end: 55
orientation: inline

LuxR/HSL
type: SequenceAnnotation
persistent identity: annotation2045
display id: annotation2045
version: 1
top level: lux pR
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0062/annotation2045/range2045/1

https://synbiohub.org/public/igem/BBa_R0062/annotation2045/range2045/1
type: Range
persistent identity: range2045
display id: range2045
version: 1
direction: direction/forward
top level: lux pR
start: 1
end: 20
orientation: inline

BBa_J202011
type: ComponentDefinition
persistent identity: BBa J202011
display id: BBa_J202011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PBAD Mutant#12
creator: Jiyeon Park
created: 2012-07-25T11:00:00Z
modified: 2015-08-31T04:08:37Z
discontinued: false
dominant: false
group u list: _910_
m user id: 0
owner id: 11164
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is PBAD (BBa_I13453) mutant. -56 relative to the araC transcriptional start site was changed from A to G.
mutable notes: In order to regulate transcription from PBAD, araC binds three different sites called araO2, araI1, and araI2. In the absence of L-arabinose, araC binds both araO2 and araI1 and bends DNA preventing transcription from PBAD. In the presence of L-arabinose, however, araC has a conformational change because of L-arabinose and binds araI1 and araI2, enabling transcription from PBAD. Like the wild-type (I13453), this part does not have araO2 site which is essential for araC repressor function. Therefore, araC acts as an activator in the presence of L-arabinose.
mutable provenance: This mutant was created by site-directed mutagenesis.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Mutation
sequence annotation: Transcription Start
sequence annotation: araI2
sequence annotation: araI1
sequence: https://synbiohub.org/public/igem/BBa_J202011_sequence/1

Mutation
type: SequenceAnnotation
persistent identity: annotation2178461
display id: annotation2178461
version: 1
top level: BBa_J202011
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_J202011/annotation2178461/range2178461/1

https://synbiohub.org/public/igem/BBa_J202011/annotation2178461/range2178461/1
type: Range
persistent identity: range2178461
display id: range2178461
version: 1
direction: direction/forward
top level: BBa_J202011
start: 56
end: 56
orientation: inline

Transcription Start
type: SequenceAnnotation
persistent identity: annotation2178462
display id: annotation2178462
version: 1
top level: BBa_J202011
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_J202011/annotation2178462/range2178462/1

https://synbiohub.org/public/igem/BBa_J202011/annotation2178462/range2178462/1
type: Range
persistent identity: range2178462
display id: range2178462
version: 1
direction: direction/forward
top level: BBa_J202011
start: 112
end: 112
orientation: inline

araI2
type: SequenceAnnotation
persistent identity: annotation2178464
display id: annotation2178464
version: 1
top level: BBa_J202011
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J202011/annotation2178464/range2178464/1

https://synbiohub.org/public/igem/BBa_J202011/annotation2178464/range2178464/1
type: Range
persistent identity: range2178464
display id: range2178464
version: 1
direction: direction/forward
top level: BBa_J202011
start: 61
end: 77
orientation: inline

araI1
type: SequenceAnnotation
persistent identity: annotation2178463
display id: annotation2178463
version: 1
top level: BBa_J202011
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J202011/annotation2178463/range2178463/1

https://synbiohub.org/public/igem/BBa_J202011/annotation2178463/range2178463/1
type: Range
persistent identity: range2178463
display id: range2178463
version: 1
direction: direction/forward
top level: BBa_J202011
start: 40
end: 56
orientation: inline

tetR
type: ComponentDefinition
persistent identity: BBa C0040
display id: BBa_C0040
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: tetracycline repressor from transposon Tn10 (+LVA)
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman.
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Coding region for the TetR protein without the Ribosome Binding Site. Modified with an LVA tail for rapid degradation of the protein and faster fall time for the emission. TetR binds to the pTet regulator (BBa_R0040). aTc (anhydrotetracycline) binds to TetR and inhibits its operation.
mutable notes: References (unparsed) here: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999). Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10. PMID: 9092630 References (unparsed) here: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999). Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10. PMID: 9092630 BBa_C0040 TetR Protein is based on the TetR sequence from Elowitz's repressilator. It has been modified to include a rapid degradation LVA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.
mutable provenance: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999)
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: tetR
sequence annotation: SsrA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0040_sequence/1

tetR
type: SequenceAnnotation
persistent identity: annotation23329
display id: annotation23329
version: 1
top level: tetR
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0040/annotation23329/range23329/1

https://synbiohub.org/public/igem/BBa_C0040/annotation23329/range23329/1
type: Range
persistent identity: range23329
display id: range23329
version: 1
direction: direction/forward
top level: tetR
start: 4
end: 620
orientation: inline

SsrA
type: SequenceAnnotation
persistent identity: annotation23330
display id: annotation23330
version: 1
top level: tetR
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_C0040/annotation23330/range23330/1

https://synbiohub.org/public/igem/BBa_C0040/annotation23330/range23330/1
type: Range
persistent identity: range23330
display id: range23330
version: 1
direction: direction/forward
top level: tetR
start: 621
end: 654
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213989
display id: annotation2213989
version: 1
top level: tetR
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_C0040/annotation2213989/range2213989/1

https://synbiohub.org/public/igem/BBa_C0040/annotation2213989/range2213989/1
type: Range
persistent identity: range2213989
display id: range2213989
version: 1
direction: direction/forward
top level: tetR
start: 661
end: 685
orientation: inline

BBa_K598014
type: ComponentDefinition
persistent identity: BBa K598014
display id: BBa_K598014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pBAD+HH+CdIntron+E0040+b0015
creator: Xiaowei YAN, Tong MU
created: 2011-09-26T11:00:00Z
modified: 2015-05-08T01:12:50Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _770_
m user id: 0
owner id: 8713
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This a GFP reporter regulated by a self-cleaving hammerhead ribozyme. The self-cleaving of the hammerhead ribozyme would lead to the formation of the intron P1 stem, thus triggering the self-splicing of the intron.
mutable notes: The self-cleaving of the hammerhead ribozyme would lead to the formation of the intron P1 stem, thus triggering the self-splicing of the intron.
mutable provenance: The intron derives from the genome of Cd
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: HH
sequence annotation: eGFP
sequence annotation: RBS
sequence annotation: pBAD
sequence annotation: BBa_B0015
sequence annotation: group I intron
sequence: https://synbiohub.org/public/igem/BBa_K598014_sequence/1

HH
type: SequenceAnnotation
persistent identity: annotation2153082
display id: annotation2153082
version: 1
top level: BBa_K598014
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K598014/annotation2153082/range2153082/1

https://synbiohub.org/public/igem/BBa_K598014/annotation2153082/range2153082/1
type: Range
persistent identity: range2153082
display id: range2153082
version: 1
direction: direction/forward
top level: BBa_K598014
start: 139
end: 185
orientation: inline

eGFP
type: SequenceAnnotation
persistent identity: annotation2153086
display id: annotation2153086
version: 1
top level: BBa_K598014
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K598014/annotation2153086/range2153086/1

https://synbiohub.org/public/igem/BBa_K598014/annotation2153086/range2153086/1
type: Range
persistent identity: range2153086
display id: range2153086
version: 1
direction: direction/forward
top level: BBa_K598014
start: 765
end: 1484
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2153089
display id: annotation2153089
version: 1
top level: BBa_K598014
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K598014/annotation2153089/range2153089/1

https://synbiohub.org/public/igem/BBa_K598014/annotation2153089/range2153089/1
type: Range
persistent identity: range2153089
display id: range2153089
version: 1
direction: direction/forward
top level: BBa_K598014
start: 758
end: 758
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2153088
display id: annotation2153088
version: 1
top level: BBa_K598014
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K598014/annotation2153088/range2153088/1

https://synbiohub.org/public/igem/BBa_K598014/annotation2153088/range2153088/1
type: Range
persistent identity: range2153088
display id: range2153088
version: 1
direction: direction/forward
top level: BBa_K598014
start: 187
end: 191
orientation: inline

pBAD
type: SequenceAnnotation
persistent identity: annotation2153081
display id: annotation2153081
version: 1
top level: BBa_K598014
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K598014/annotation2153081/range2153081/1

https://synbiohub.org/public/igem/BBa_K598014/annotation2153081/range2153081/1
type: Range
persistent identity: range2153081
display id: range2153081
version: 1
direction: direction/forward
top level: BBa_K598014
start: 1
end: 130
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2153087
display id: annotation2153087
version: 1
top level: BBa_K598014
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K598014/annotation2153087/range2153087/1

https://synbiohub.org/public/igem/BBa_K598014/annotation2153087/range2153087/1
type: Range
persistent identity: range2153087
display id: range2153087
version: 1
direction: direction/forward
top level: BBa_K598014
start: 1493
end: 1621
orientation: inline

group I intron
type: SequenceAnnotation
persistent identity: annotation2153083
display id: annotation2153083
version: 1
top level: BBa_K598014
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K598014/annotation2153083/range2153083/1

https://synbiohub.org/public/igem/BBa_K598014/annotation2153083/range2153083/1
type: Range
persistent identity: range2153083
display id: range2153083
version: 1
direction: direction/forward
top level: BBa_K598014
start: 186
end: 764
orientation: inline

BBa_K864444
type: ComponentDefinition
persistent identity: BBa K864444
display id: BBa_K864444
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RFP-Linker-SYFP2
creator: Joel ??s
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1124_
m user id: 0
owner id: 13998
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Use this part to insert any 5?????TR of your gene of interest to screen for downregulation of your gene with small RNA. PCR amplify your 5???UTR with EcoRI and BamHI and clone into any backbone carrying this part. Digest whis part with EcoRI and BamHI and as RFP is replaced, this part functions as a red/white screening system. This allows you to create a reporter system with a fluorescent marker for screening for functional small RNAs. SYFP2 BBa_K864100 is situated downstrean of the RFP BBa_J04450 and is connected in frame with a 12 aa glycine-serine repeated linker BBa_J18922 , to prevent folding distruptions in the YFP or the 5???UTR. The first 6 nt of the glycine-serine linker is a BamHI restriction site (GGATCC), which translates into glycine-serine in E.Coli. When screening for working small RNAs, it's recommended to include the first 15 codons of the gene of interest.
mutable notes: -
mutable provenance: Constructed from SYFP2 BBa_K864100 , linker BBa_J18922 and RFP BBa_J04450
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: SYFP2
sequence annotation: B0012
sequence annotation: RFP J04450
sequence annotation: BamHI
sequence annotation: Plac
sequence annotation: B0034
sequence annotation: B0010
sequence annotation: RFP
sequence annotation: Linker
sequence: https://synbiohub.org/public/igem/BBa_K864444_sequence/1

SYFP2
type: SequenceAnnotation
persistent identity: annotation2198896
display id: annotation2198896
version: 1
top level: BBa_K864444
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K864444/annotation2198896/range2198896/1

https://synbiohub.org/public/igem/BBa_K864444/annotation2198896/range2198896/1
type: Range
persistent identity: range2198896
display id: range2198896
version: 1
direction: direction/forward
top level: BBa_K864444
start: 1106
end: 1825
orientation: inline

B0012
type: SequenceAnnotation
persistent identity: annotation2198889
display id: annotation2198889
version: 1
top level: BBa_K864444
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K864444/annotation2198889/range2198889/1

https://synbiohub.org/public/igem/BBa_K864444/annotation2198889/range2198889/1
type: Range
persistent identity: range2198889
display id: range2198889
version: 1
direction: direction/forward
top level: BBa_K864444
start: 1029
end: 1069
orientation: inline

RFP J04450
type: SequenceAnnotation
persistent identity: annotation2198883
display id: annotation2198883
version: 1
top level: BBa_K864444
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864444/annotation2198883/range2198883/1

https://synbiohub.org/public/igem/BBa_K864444/annotation2198883/range2198883/1
type: Range
persistent identity: range2198883
display id: range2198883
version: 1
direction: direction/forward
top level: BBa_K864444
start: 1
end: 1068
orientation: inline

BamHI
type: SequenceAnnotation
persistent identity: annotation2198894
display id: annotation2198894
version: 1
top level: BBa_K864444
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864444/annotation2198894/range2198894/1

https://synbiohub.org/public/igem/BBa_K864444/annotation2198894/range2198894/1
type: Range
persistent identity: range2198894
display id: range2198894
version: 1
direction: direction/forward
top level: BBa_K864444
start: 1070
end: 1075
orientation: inline

Plac
type: SequenceAnnotation
persistent identity: annotation2198885
display id: annotation2198885
version: 1
top level: BBa_K864444
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K864444/annotation2198885/range2198885/1

https://synbiohub.org/public/igem/BBa_K864444/annotation2198885/range2198885/1
type: Range
persistent identity: range2198885
display id: range2198885
version: 1
direction: direction/forward
top level: BBa_K864444
start: 1
end: 200
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2198886
display id: annotation2198886
version: 1
top level: BBa_K864444
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K864444/annotation2198886/range2198886/1

https://synbiohub.org/public/igem/BBa_K864444/annotation2198886/range2198886/1
type: Range
persistent identity: range2198886
display id: range2198886
version: 1
direction: direction/forward
top level: BBa_K864444
start: 209
end: 220
orientation: inline

B0010
type: SequenceAnnotation
persistent identity: annotation2198890
display id: annotation2198890
version: 1
top level: BBa_K864444
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K864444/annotation2198890/range2198890/1

https://synbiohub.org/public/igem/BBa_K864444/annotation2198890/range2198890/1
type: Range
persistent identity: range2198890
display id: range2198890
version: 1
direction: direction/forward
top level: BBa_K864444
start: 941
end: 1020
orientation: inline

RFP
type: SequenceAnnotation
persistent identity: annotation2198887
display id: annotation2198887
version: 1
top level: BBa_K864444
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K864444/annotation2198887/range2198887/1

https://synbiohub.org/public/igem/BBa_K864444/annotation2198887/range2198887/1
type: Range
persistent identity: range2198887
display id: range2198887
version: 1
direction: direction/forward
top level: BBa_K864444
start: 227
end: 681
orientation: inline

Linker
type: SequenceAnnotation
persistent identity: annotation2198893
display id: annotation2198893
version: 1
top level: BBa_K864444
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864444/annotation2198893/range2198893/1

https://synbiohub.org/public/igem/BBa_K864444/annotation2198893/range2198893/1
type: Range
persistent identity: range2198893
display id: range2198893
version: 1
direction: direction/forward
top level: BBa_K864444
start: 1076
end: 1105
orientation: inline

BBa_I718009
type: ComponentDefinition
persistent identity: BBa I718009
display id: BBa_I718009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pTet-lox71-rbs-gfp-ter
creator: David Bikard
created: 2007-10-25T11:00:00Z
modified: 2015-08-31T04:07:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _141_
m user id: 0
owner id: 1481
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Upstream construct of the synthetic multicellular bacteria
mutable notes: none
mutable provenance: none
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_B0012
component: BBa_E0040
component: BBa_B0030
component: BBa_B0010
component: BBa_R0040
component: BBa_J37028
sequence annotation: BBa_B0012
sequence annotation: BBa_R0040
sequence annotation: BBa_J37028
sequence annotation: BBa_B0010
sequence annotation: BBa_B0030
sequence annotation: BBa_E0040
sequence: https://synbiohub.org/public/igem/BBa_I718009_sequence/1

BBa_B0012
type: Component
persistent identity: component1956135
display id: component1956135
version: 1
top level: BBa_I718009
access: public
definition: BBa_B0012

BBa_E0040
type: Component
persistent identity: component1956132
display id: component1956132
version: 1
top level: BBa_I718009
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_B0030
type: Component
persistent identity: component1956129
display id: component1956129
version: 1
top level: BBa_I718009
access: public
definition: BBa_B0030

BBa_B0010
type: Component
persistent identity: component1956133
display id: component1956133
version: 1
top level: BBa_I718009
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_R0040
type: Component
persistent identity: component1956123
display id: component1956123
version: 1
top level: BBa_I718009
access: public
definition: p(tetR)

BBa_J37028
type: Component
persistent identity: component1956127
display id: component1956127
version: 1
top level: BBa_I718009
access: public
definition: https://synbiohub.org/public/igem/BBa_J37028/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1956135
display id: annotation1956135
version: 1
top level: BBa_I718009
location: https://synbiohub.org/public/igem/BBa_I718009/annotation1956135/range1956135/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I718009/annotation1956135/range1956135/1
type: Range
persistent identity: range1956135
display id: range1956135
version: 1
direction: direction/forward
top level: BBa_I718009
start: 942
end: 982
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1956123
display id: annotation1956123
version: 1
top level: BBa_I718009
location: https://synbiohub.org/public/igem/BBa_I718009/annotation1956123/range1956123/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_I718009/annotation1956123/range1956123/1
type: Range
persistent identity: range1956123
display id: range1956123
version: 1
direction: direction/forward
top level: BBa_I718009
start: 1
end: 54
orientation: inline

BBa_J37028
type: SequenceAnnotation
persistent identity: annotation1956127
display id: annotation1956127
version: 1
top level: BBa_I718009
location: https://synbiohub.org/public/igem/BBa_I718009/annotation1956127/range1956127/1
component: BBa_J37028

https://synbiohub.org/public/igem/BBa_I718009/annotation1956127/range1956127/1
type: Range
persistent identity: range1956127
display id: range1956127
version: 1
direction: direction/reverse
top level: BBa_I718009
start: 63
end: 96
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1956133
display id: annotation1956133
version: 1
top level: BBa_I718009
location: https://synbiohub.org/public/igem/BBa_I718009/annotation1956133/range1956133/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I718009/annotation1956133/range1956133/1
type: Range
persistent identity: range1956133
display id: range1956133
version: 1
direction: direction/forward
top level: BBa_I718009
start: 854
end: 933
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1956129
display id: annotation1956129
version: 1
top level: BBa_I718009
location: https://synbiohub.org/public/igem/BBa_I718009/annotation1956129/range1956129/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_I718009/annotation1956129/range1956129/1
type: Range
persistent identity: range1956129
display id: range1956129
version: 1
direction: direction/forward
top level: BBa_I718009
start: 105
end: 119
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1956132
display id: annotation1956132
version: 1
top level: BBa_I718009
location: https://synbiohub.org/public/igem/BBa_I718009/annotation1956132/range1956132/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_I718009/annotation1956132/range1956132/1
type: Range
persistent identity: range1956132
display id: range1956132
version: 1
direction: direction/reverse
top level: BBa_I718009
start: 126
end: 845
orientation: inline

BBa_K769020
type: ComponentDefinition
persistent identity: BBa K769020
display id: BBa_K769020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: lux operon(K769011) + lumP(K769019)
creator: Tomomi YOKOYAMA
created: 2012-09-18T11:00:00Z
modified: 2015-05-08T01:13:14Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1021_
m user id: 0
owner id: 14294
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Promoter(Pbad)-lux operon-DT + lumP(K769019)
mutable notes: blue shift
mutable provenance: P.kishitanii
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K769011
component: BBa_K769019
sequence annotation: BBa_K769011
sequence annotation: BBa_K769019
sequence: https://synbiohub.org/public/igem/BBa_K769020_sequence/1

BBa_K769011
type: Component
persistent identity: component2186406
display id: component2186406
version: 1
top level: BBa_K769020
access: public
definition: https://synbiohub.org/public/igem/BBa_K769011/1

BBa_K769019
type: Component
persistent identity: component2186418
display id: component2186418
version: 1
top level: BBa_K769020
access: public
definition: https://synbiohub.org/public/igem/BBa_K769019/1

BBa_K769011
type: SequenceAnnotation
persistent identity: annotation2186406
display id: annotation2186406
version: 1
top level: BBa_K769020
location: https://synbiohub.org/public/igem/BBa_K769020/annotation2186406/range2186406/1
component: BBa_K769011

https://synbiohub.org/public/igem/BBa_K769020/annotation2186406/range2186406/1
type: Range
persistent identity: range2186406
display id: range2186406
version: 1
direction: direction/reverse
top level: BBa_K769020
start: 1
end: 8531
orientation: inline

BBa_K769019
type: SequenceAnnotation
persistent identity: annotation2186418
display id: annotation2186418
version: 1
top level: BBa_K769020
location: https://synbiohub.org/public/igem/BBa_K769020/annotation2186418/range2186418/1
component: BBa_K769019

https://synbiohub.org/public/igem/BBa_K769020/annotation2186418/range2186418/1
type: Range
persistent identity: range2186418
display id: range2186418
version: 1
direction: direction/reverse
top level: BBa_K769020
start: 8540
end: 9301
orientation: inline

BBa_K567008
type: ComponentDefinition
persistent identity: BBa K567008
display id: BBa_K567008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PT7-Luc-2AGG
creator: Yunfeng Ruan and Chunying Li
created: 2011-09-28T11:00:00Z
modified: 2015-05-08T01:12:43Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _735_
m user id: 0
owner id: 8782
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This biobrick is constructed by putting modified enzyme luciferase under promoter T7 and controlled by lac operator. 2 AGG codons and 2 GCG codons are inserted after the ATG start codon of wild type luciferase (BBa_I712019). Modified luciferase keeps the activity of converting luciferin into oxyluciferin, during which bioluminescence will emit. This part is one of the reporter genes to testify the influence of different number of rare codons in regulating protein biosynthesis. This part is used as a measurement to testify the function of LacI -Ptac-tRNA(Arg) (BBa_K567001) or sulA promoter-tRNA(Arg) (BBa_K567002). Cell is cultured in 50ug/ml kanamycin 10ug/ml tetracycline LB liquid medium. When the OD600 of the culture reaches 0.3 IPTG is added to make the final concentration 0.5nM to induce the synthesis of tRNA. Ultrasonication is used to release the luciferase from the cell. Sonics ON 3 seconds, OFF 3 seconds, total ultrasonication time 3minutes. Amount of bioluminescence produced can be detected using luminometer.
mutable notes: PCR is used to insert the 2 AGG codons into the gene after ATG.
mutable provenance: T7 promoter is derived from pET28A. Wild type Luciferase from BBa_I712019.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: PT7
sequence annotation: luciferase
sequence annotation: lac operator
sequence annotation: rbs
sequence annotation: 2AGG
sequence annotation: ATG
sequence: https://synbiohub.org/public/igem/BBa_K567008_sequence/1

PT7
type: SequenceAnnotation
persistent identity: annotation2150784
display id: annotation2150784
version: 1
top level: BBa_K567008
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K567008/annotation2150784/range2150784/1

https://synbiohub.org/public/igem/BBa_K567008/annotation2150784/range2150784/1
type: Range
persistent identity: range2150784
display id: range2150784
version: 1
direction: direction/forward
top level: BBa_K567008
start: 2
end: 18
orientation: inline

luciferase
type: SequenceAnnotation
persistent identity: annotation2150771
display id: annotation2150771
version: 1
top level: BBa_K567008
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K567008/annotation2150771/range2150771/1

https://synbiohub.org/public/igem/BBa_K567008/annotation2150771/range2150771/1
type: Range
persistent identity: range2150771
display id: range2150771
version: 1
direction: direction/forward
top level: BBa_K567008
start: 89
end: 1756
orientation: inline

lac operator
type: SequenceAnnotation
persistent identity: annotation2150785
display id: annotation2150785
version: 1
top level: BBa_K567008
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K567008/annotation2150785/range2150785/1

https://synbiohub.org/public/igem/BBa_K567008/annotation2150785/range2150785/1
type: Range
persistent identity: range2150785
display id: range2150785
version: 1
direction: direction/forward
top level: BBa_K567008
start: 21
end: 44
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2150787
display id: annotation2150787
version: 1
top level: BBa_K567008
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K567008/annotation2150787/range2150787/1

https://synbiohub.org/public/igem/BBa_K567008/annotation2150787/range2150787/1
type: Range
persistent identity: range2150787
display id: range2150787
version: 1
direction: direction/forward
top level: BBa_K567008
start: 75
end: 81
orientation: inline

2AGG
type: SequenceAnnotation
persistent identity: annotation2150770
display id: annotation2150770
version: 1
top level: BBa_K567008
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K567008/annotation2150770/range2150770/1

https://synbiohub.org/public/igem/BBa_K567008/annotation2150770/range2150770/1
type: Range
persistent identity: range2150770
display id: range2150770
version: 1
direction: direction/forward
top level: BBa_K567008
start: 95
end: 100
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2150769
display id: annotation2150769
version: 1
top level: BBa_K567008
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K567008/annotation2150769/range2150769/1

https://synbiohub.org/public/igem/BBa_K567008/annotation2150769/range2150769/1
type: Range
persistent identity: range2150769
display id: range2150769
version: 1
direction: direction/forward
top level: BBa_K567008
start: 89
end: 91
orientation: inline

BBa_K876070
type: ComponentDefinition
persistent identity: BBa K876070
display id: BBa_K876070
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CP-GFP-Term
creator: Zakaraya Ashour, Sanket Shah
created: 2012-10-01T11:00:00Z
modified: 2015-05-08T01:13:39Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1138_
m user id: 0
owner id: 13835
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Constitutive promoter with GFP
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_J23103
component: BBa_K081012
sequence annotation: BBa_J23103
sequence annotation: BBa_K081012
sequence: https://synbiohub.org/public/igem/BBa_K876070_sequence/1

BBa_J23103
type: Component
persistent identity: component2206856
display id: component2206856
version: 1
top level: BBa_K876070
access: public
definition: https://synbiohub.org/public/igem/BBa_J23103/1

BBa_K081012
type: Component
persistent identity: component2206867
display id: component2206867
version: 1
top level: BBa_K876070
access: public
definition: https://synbiohub.org/public/igem/BBa_K081012/1

BBa_J23103
type: SequenceAnnotation
persistent identity: annotation2206856
display id: annotation2206856
version: 1
top level: BBa_K876070
location: https://synbiohub.org/public/igem/BBa_K876070/annotation2206856/range2206856/1
component: BBa_J23103

https://synbiohub.org/public/igem/BBa_K876070/annotation2206856/range2206856/1
type: Range
persistent identity: range2206856
display id: range2206856
version: 1
direction: direction/reverse
top level: BBa_K876070
start: 1
end: 35
orientation: inline

BBa_K081012
type: SequenceAnnotation
persistent identity: annotation2206867
display id: annotation2206867
version: 1
top level: BBa_K876070
location: https://synbiohub.org/public/igem/BBa_K876070/annotation2206867/range2206867/1
component: BBa_K081012

https://synbiohub.org/public/igem/BBa_K876070/annotation2206867/range2206867/1
type: Range
persistent identity: range2206867
display id: range2206867
version: 1
direction: direction/reverse
top level: BBa_K876070
start: 44
end: 831
orientation: inline

BBa_I714044
type: ComponentDefinition
persistent identity: BBa I714044
display id: BBa_I714044
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [R0010][I714031]
creator: Mingzhi Qu
created: 2007-10-20T11:00:00Z
modified: 2015-08-31T04:07:47Z
discontinued: false
dominant: false
group u list: _142_
m user id: 0
owner id: 2248
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is I714031 with R0010 Promoter in the front.
mutable provenance: [R0010]+[I714031]
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_I714031
component: BBa_R0010
sequence annotation: BBa_R0010
sequence annotation: BBa_I714031
sequence: https://synbiohub.org/public/igem/BBa_I714044_sequence/1

BBa_I714031
type: Component
persistent identity: component1951661
display id: component1951661
version: 1
top level: BBa_I714044
access: public
definition: BBa_I714031

BBa_R0010
type: Component
persistent identity: component1951654
display id: component1951654
version: 1
top level: BBa_I714044
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1951654
display id: annotation1951654
version: 1
top level: BBa_I714044
location: https://synbiohub.org/public/igem/BBa_I714044/annotation1951654/range1951654/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_I714044/annotation1951654/range1951654/1
type: Range
persistent identity: range1951654
display id: range1951654
version: 1
direction: direction/forward
top level: BBa_I714044
start: 1
end: 200
orientation: inline

BBa_I714031
type: SequenceAnnotation
persistent identity: annotation1951661
display id: annotation1951661
version: 1
top level: BBa_I714044
location: https://synbiohub.org/public/igem/BBa_I714044/annotation1951661/range1951661/1
component: BBa_I714031

https://synbiohub.org/public/igem/BBa_I714044/annotation1951661/range1951661/1
type: Range
persistent identity: range1951661
display id: range1951661
version: 1
direction: direction/reverse
top level: BBa_I714044
start: 209
end: 486
orientation: inline

BBa_K863001
type: ComponentDefinition
persistent identity: BBa K863001
display id: BBa_K863001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: bpul laccase from Bacillus pumilus
creator: Isabel Huber
created: 2012-09-17T11:00:00Z
modified: 2015-05-08T01:13:36Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1123_
m user id: 0
owner id: 12844
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: bpul (Laccase from Bacillus pumilus)
mutable notes: test
mutable provenance: Bacillus pumilus DSM 27 (ATCC7061)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: bpul
sequence annotation: ATG
sequence annotation: A (Original) - T (Mutation)
sequence annotation: TAA
sequence: https://synbiohub.org/public/igem/BBa_K863001_sequence/1

bpul
type: SequenceAnnotation
persistent identity: annotation2184621
display id: annotation2184621
version: 1
top level: BBa_K863001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K863001/annotation2184621/range2184621/1

https://synbiohub.org/public/igem/BBa_K863001/annotation2184621/range2184621/1
type: Range
persistent identity: range2184621
display id: range2184621
version: 1
direction: direction/forward
top level: BBa_K863001
start: 1
end: 1533
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2184622
display id: annotation2184622
version: 1
top level: BBa_K863001
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K863001/annotation2184622/range2184622/1

https://synbiohub.org/public/igem/BBa_K863001/annotation2184622/range2184622/1
type: Range
persistent identity: range2184622
display id: range2184622
version: 1
direction: direction/forward
top level: BBa_K863001
start: 1
end: 3
orientation: inline

A (Original) - T (Mutation)
type: SequenceAnnotation
persistent identity: annotation2184905
display id: annotation2184905
version: 1
top level: BBa_K863001
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K863001/annotation2184905/range2184905/1

https://synbiohub.org/public/igem/BBa_K863001/annotation2184905/range2184905/1
type: Range
persistent identity: range2184905
display id: range2184905
version: 1
direction: direction/forward
top level: BBa_K863001
start: 801
end: 801
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2184641
display id: annotation2184641
version: 1
top level: BBa_K863001
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K863001/annotation2184641/range2184641/1

https://synbiohub.org/public/igem/BBa_K863001/annotation2184641/range2184641/1
type: Range
persistent identity: range2184641
display id: range2184641
version: 1
direction: direction/forward
top level: BBa_K863001
start: 1531
end: 1533
orientation: inline

BBa_B0023
type: ComponentDefinition
persistent identity: BBa B0023
display id: BBa_B0023
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TE from coliphage T7, reversed
creator: Caitlin Conboy
created: 2003-10-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part is the reverse of terminator B0013, a bacteriophage T7 terminator which was engineered to be bidirectional. Contains a poly A insertion upstream of a 20 bp stem-loop.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence: https://synbiohub.org/public/igem/BBa_B0023_sequence/1

BBa_J13023
type: ComponentDefinition
persistent identity: BBa J13023
display id: BBa_J13023
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 3OC6HSL+LuxR dependent POPS/RIPS generator
creator: Jeff Tabor
created: 2005-06-28T11:00:00Z
modified: 2015-08-31T04:08:29Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _37_
m user id: 0
owner id: 88
owning group id: 37
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: produces pops and rips in the presence of luxR/3OC6HSL and the absence of lambda cI.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_R0065
component: BBa_B0034
sequence annotation: BBa_R0065
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_J13023_sequence/1

BBa_R0065
type: Component
persistent identity: component1556571
display id: component1556571
version: 1
top level: BBa_J13023
access: public
definition: cI+luxR

BBa_B0034
type: Component
persistent identity: component1556583
display id: component1556583
version: 1
top level: BBa_J13023
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0065
type: SequenceAnnotation
persistent identity: annotation1556571
display id: annotation1556571
version: 1
top level: BBa_J13023
location: https://synbiohub.org/public/igem/BBa_J13023/annotation1556571/range1556571/1
component: BBa_R0065

https://synbiohub.org/public/igem/BBa_J13023/annotation1556571/range1556571/1
type: Range
persistent identity: range1556571
display id: range1556571
version: 1
direction: direction/forward
top level: BBa_J13023
start: 1
end: 97
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1556583
display id: annotation1556583
version: 1
top level: BBa_J13023
location: https://synbiohub.org/public/igem/BBa_J13023/annotation1556583/range1556583/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J13023/annotation1556583/range1556583/1
type: Range
persistent identity: range1556583
display id: range1556583
version: 1
direction: direction/forward
top level: BBa_J13023
start: 106
end: 117
orientation: inline

BBa_I732904
type: ComponentDefinition
persistent identity: BBa I732904
display id: BBa_I732904
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: R0040 B0015
creator: Zhan Jian
created: 2007-10-16T11:00:00Z
modified: 2015-08-31T04:08:00Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_R0040
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_R0040
sequence: https://synbiohub.org/public/igem/BBa_I732904_sequence/1

BBa_R0040
type: Component
persistent identity: component1948588
display id: component1948588
version: 1
top level: BBa_I732904
access: public
definition: p(tetR)

BBa_B0012
type: Component
persistent identity: component1948594
display id: component1948594
version: 1
top level: BBa_I732904
access: public
definition: BBa_B0012

BBa_B0010
type: Component
persistent identity: component1948592
display id: component1948592
version: 1
top level: BBa_I732904
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1948594
display id: annotation1948594
version: 1
top level: BBa_I732904
location: https://synbiohub.org/public/igem/BBa_I732904/annotation1948594/range1948594/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I732904/annotation1948594/range1948594/1
type: Range
persistent identity: range1948594
display id: range1948594
version: 1
direction: direction/forward
top level: BBa_I732904
start: 151
end: 191
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1948592
display id: annotation1948592
version: 1
top level: BBa_I732904
location: https://synbiohub.org/public/igem/BBa_I732904/annotation1948592/range1948592/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I732904/annotation1948592/range1948592/1
type: Range
persistent identity: range1948592
display id: range1948592
version: 1
direction: direction/forward
top level: BBa_I732904
start: 63
end: 142
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1948588
display id: annotation1948588
version: 1
top level: BBa_I732904
location: https://synbiohub.org/public/igem/BBa_I732904/annotation1948588/range1948588/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_I732904/annotation1948588/range1948588/1
type: Range
persistent identity: range1948588
display id: range1948588
version: 1
direction: direction/forward
top level: BBa_I732904
start: 1
end: 54
orientation: inline

BBa_K911003
type: ComponentDefinition
persistent identity: BBa K911003
display id: BBa_K911003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Fluoride Sensitive Riboswitch
creator: Oliver Meacock
created: 2012-09-11T11:00:00Z
modified: 2015-05-08T01:13:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1176_
m user id: 0
owner id: 12694
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Riboswitch that is highly sensitive to the F- ion. We have characterized this part in three different chassis: TOP10 e.coli, Laboratory strain bacillus subtilis and a strain of bacillus subtilis with its normal fluoride riboswitch knocked out (kindly provided by the Breaker lab in Yale). Results of Miller assays for these three chassis are shown below.
mutable notes: Because this is a positive regulator (it ceases transcriptional termination in the presence of F- ions), it was decided that it should be placed upstream of a direct reporter, in this case lacZ as our reporter.
mutable provenance: Bacillus Cereus Genome
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
sequence annotation: Fluoride riboswitch
sequence annotation: Lysine promoter
sequence: https://synbiohub.org/public/igem/BBa_K911003_sequence/1

Fluoride riboswitch
type: SequenceAnnotation
persistent identity: annotation2182733
display id: annotation2182733
version: 1
top level: BBa_K911003
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K911003/annotation2182733/range2182733/1

https://synbiohub.org/public/igem/BBa_K911003/annotation2182733/range2182733/1
type: Range
persistent identity: range2182733
display id: range2182733
version: 1
direction: direction/forward
top level: BBa_K911003
start: 61
end: 139
orientation: inline

Lysine promoter
type: SequenceAnnotation
persistent identity: annotation2182732
display id: annotation2182732
version: 1
top level: BBa_K911003
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K911003/annotation2182732/range2182732/1

https://synbiohub.org/public/igem/BBa_K911003/annotation2182732/range2182732/1
type: Range
persistent identity: range2182732
display id: range2182732
version: 1
direction: direction/forward
top level: BBa_K911003
start: 1
end: 43
orientation: inline

BBa_K773003
type: ComponentDefinition
persistent identity: BBa K773003
display id: BBa_K773003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: mCherry-LVA
creator: Katie Knister
created: 2012-09-30T11:00:00Z
modified: 2015-05-08T01:13:15Z
discontinued: false
dominant: false
group u list: _1025_
m user id: 0
owner id: 11646
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part contains the fluorescent protein mCherry attached to a degradation tag. It will decrease the amount of fluorescent protein over time.
mutable notes: We modified the AAV sequence to LVA with PCR.
mutable provenance: This part was modified from a plasmid provided by the Murray lab at Caltech.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: Stop
sequence annotation: mCherry-LVA
sequence annotation: B0034
sequence annotation: ssrA degradation tag: LVA
sequence annotation: Start
sequence: https://synbiohub.org/public/igem/BBa_K773003_sequence/1

Stop
type: SequenceAnnotation
persistent identity: annotation2209512
display id: annotation2209512
version: 1
top level: BBa_K773003
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K773003/annotation2209512/range2209512/1

https://synbiohub.org/public/igem/BBa_K773003/annotation2209512/range2209512/1
type: Range
persistent identity: range2209512
display id: range2209512
version: 1
direction: direction/forward
top level: BBa_K773003
start: 760
end: 762
orientation: inline

Stop
type: SequenceAnnotation
persistent identity: annotation2209513
display id: annotation2209513
version: 1
top level: BBa_K773003
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K773003/annotation2209513/range2209513/1

https://synbiohub.org/public/igem/BBa_K773003/annotation2209513/range2209513/1
type: Range
persistent identity: range2209513
display id: range2209513
version: 1
direction: direction/forward
top level: BBa_K773003
start: 763
end: 765
orientation: inline

mCherry-LVA
type: SequenceAnnotation
persistent identity: annotation2209509
display id: annotation2209509
version: 1
top level: BBa_K773003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K773003/annotation2209509/range2209509/1

https://synbiohub.org/public/igem/BBa_K773003/annotation2209509/range2209509/1
type: Range
persistent identity: range2209509
display id: range2209509
version: 1
direction: direction/forward
top level: BBa_K773003
start: 19
end: 765
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2209508
display id: annotation2209508
version: 1
top level: BBa_K773003
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K773003/annotation2209508/range2209508/1

https://synbiohub.org/public/igem/BBa_K773003/annotation2209508/range2209508/1
type: Range
persistent identity: range2209508
display id: range2209508
version: 1
direction: direction/forward
top level: BBa_K773003
start: 1
end: 12
orientation: inline

ssrA degradation tag: LVA
type: SequenceAnnotation
persistent identity: annotation2209511
display id: annotation2209511
version: 1
top level: BBa_K773003
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K773003/annotation2209511/range2209511/1

https://synbiohub.org/public/igem/BBa_K773003/annotation2209511/range2209511/1
type: Range
persistent identity: range2209511
display id: range2209511
version: 1
direction: direction/forward
top level: BBa_K773003
start: 730
end: 759
orientation: inline

Start
type: SequenceAnnotation
persistent identity: annotation2209510
display id: annotation2209510
version: 1
top level: BBa_K773003
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K773003/annotation2209510/range2209510/1

https://synbiohub.org/public/igem/BBa_K773003/annotation2209510/range2209510/1
type: Range
persistent identity: range2209510
display id: range2209510
version: 1
direction: direction/forward
top level: BBa_K773003
start: 19
end: 21
orientation: inline

OmpR
type: ComponentDefinition
persistent identity: BBa R0083
display id: BBa_R0083
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter (OmpR, positive)
creator: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
created: 2004-01-27T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Positively regulated, OmpR-controlled promoter. This promoter is derived from the upstream region of ompC. Phosphorylated OmpR binds to the operator site and activates transcription. This is a truncated version of BBa_R0082.
mutable notes: The promoter deletes the C2 and C3 regions of BBa_R0082 (bases 37-74 in BBa_R0082) and inserts an 8-base linker before the -35 and -10 signal region. The efficacy of this promoter versus the full ompC upstream region (BBa_R0082) or the ompF upstream region (BBa_R0084) is currently unknown. The a at base 41 is mutated to t to avoid XbaI site. Speculation that this was a previous assembly site.
mutable provenance: NC_000193 E. coli K12
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: C1 OmpR
sequence annotation: -10
sequence annotation: A
sequence annotation: linker
sequence annotation: -35
sequence: https://synbiohub.org/public/igem/BBa_R0083_sequence/1

C1 OmpR
type: SequenceAnnotation
persistent identity: annotation301221
display id: annotation301221
version: 1
top level: OmpR
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0083/annotation301221/range301221/1

https://synbiohub.org/public/igem/BBa_R0083/annotation301221/range301221/1
type: Range
persistent identity: range301221
display id: range301221
version: 1
direction: direction/forward
top level: OmpR
start: 13
end: 30
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation301226
display id: annotation301226
version: 1
top level: OmpR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0083/annotation301226/range301226/1

https://synbiohub.org/public/igem/BBa_R0083/annotation301226/range301226/1
type: Range
persistent identity: range301226
display id: range301226
version: 1
direction: direction/forward
top level: OmpR
start: 68
end: 73
orientation: inline

A
type: SequenceAnnotation
persistent identity: annotation306881
display id: annotation306881
version: 1
top level: OmpR
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_R0083/annotation306881/range306881/1

https://synbiohub.org/public/igem/BBa_R0083/annotation306881/range306881/1
type: Range
persistent identity: range306881
display id: range306881
version: 1
direction: direction/forward
top level: OmpR
start: 41
end: 41
orientation: inline

linker
type: SequenceAnnotation
persistent identity: annotation301223
display id: annotation301223
version: 1
top level: OmpR
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_R0083/annotation301223/range301223/1

https://synbiohub.org/public/igem/BBa_R0083/annotation301223/range301223/1
type: Range
persistent identity: range301223
display id: range301223
version: 1
direction: direction/forward
top level: OmpR
start: 37
end: 44
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation301224
display id: annotation301224
version: 1
top level: OmpR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0083/annotation301224/range301224/1

https://synbiohub.org/public/igem/BBa_R0083/annotation301224/range301224/1
type: Range
persistent identity: range301224
display id: range301224
version: 1
direction: direction/forward
top level: OmpR
start: 45
end: 50
orientation: inline

BBa_J202009
type: ComponentDefinition
persistent identity: BBa J202009
display id: BBa_J202009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PBAD Mutant#10
creator: Jiyeon Park
created: 2012-07-25T11:00:00Z
modified: 2015-08-31T04:08:37Z
discontinued: false
dominant: false
group u list: _910_
m user id: 0
owner id: 11164
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is PBAD (BBa_I13453) mutant. -59 relative to the araC transcriptional start site was changed from C to T. Also, C at -48 was changed to T.
mutable notes: In order to regulate transcription from PBAD, araC binds three different sites called araO2, araI1, and araI2. In the absence of L-arabinose, araC binds both araO2 and araI1 and bends DNA preventing transcription from PBAD. In the presence of L-arabinose, however, araC has a conformational change because of L-arabinose and binds araI1 and araI2, enabling transcription from PBAD. Like the wild-type (I13453), this part does not have araO2 site which is essential for araC repressor function. Therefore, araC acts as an activator in the presence of L-arabinose.
mutable provenance: This mutant was created by random mutagenesis.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: araI2
sequence annotation: Mutation 2
sequence annotation: Transcription Start
sequence annotation: Mutation 1
sequence annotation: araI1
sequence: https://synbiohub.org/public/igem/BBa_J202009_sequence/1

araI2
type: SequenceAnnotation
persistent identity: annotation2178456
display id: annotation2178456
version: 1
top level: BBa_J202009
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J202009/annotation2178456/range2178456/1

https://synbiohub.org/public/igem/BBa_J202009/annotation2178456/range2178456/1
type: Range
persistent identity: range2178456
display id: range2178456
version: 1
direction: direction/forward
top level: BBa_J202009
start: 61
end: 77
orientation: inline

Mutation 2
type: SequenceAnnotation
persistent identity: annotation2178453
display id: annotation2178453
version: 1
top level: BBa_J202009
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_J202009/annotation2178453/range2178453/1

https://synbiohub.org/public/igem/BBa_J202009/annotation2178453/range2178453/1
type: Range
persistent identity: range2178453
display id: range2178453
version: 1
direction: direction/forward
top level: BBa_J202009
start: 64
end: 64
orientation: inline

Transcription Start
type: SequenceAnnotation
persistent identity: annotation2178454
display id: annotation2178454
version: 1
top level: BBa_J202009
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_J202009/annotation2178454/range2178454/1

https://synbiohub.org/public/igem/BBa_J202009/annotation2178454/range2178454/1
type: Range
persistent identity: range2178454
display id: range2178454
version: 1
direction: direction/forward
top level: BBa_J202009
start: 112
end: 112
orientation: inline

Mutation 1
type: SequenceAnnotation
persistent identity: annotation2178452
display id: annotation2178452
version: 1
top level: BBa_J202009
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_J202009/annotation2178452/range2178452/1

https://synbiohub.org/public/igem/BBa_J202009/annotation2178452/range2178452/1
type: Range
persistent identity: range2178452
display id: range2178452
version: 1
direction: direction/forward
top level: BBa_J202009
start: 53
end: 53
orientation: inline

araI1
type: SequenceAnnotation
persistent identity: annotation2178455
display id: annotation2178455
version: 1
top level: BBa_J202009
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J202009/annotation2178455/range2178455/1

https://synbiohub.org/public/igem/BBa_J202009/annotation2178455/range2178455/1
type: Range
persistent identity: range2178455
display id: range2178455
version: 1
direction: direction/forward
top level: BBa_J202009
start: 40
end: 56
orientation: inline

lux pL
type: ComponentDefinition
persistent identity: BBa R0063
display id: BBa_R0063
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter (luxR & HSL regulated -- lux pL)
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: The lux cassette of V. fischeri contains a left and a right promoter. The left promoter gives weak constitutive expression of downstream genes.This expression is down-regulated by the action of the Lux repressor, LuxR. Two molecules of LuxR protein form a complex with two molecules the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription from Pr, repressing transcription from Pl
mutable notes: This promoter is based on the Vibrio fischeri quorum sensing gene promoters. Two genes LuxI and LuxR and transcribed in opposite directions as shown below. The original sequence from which the parts <bb_part>BBa_R0062</bb_part> and <bb_part>BBa_R0063</bb_part> were derived is shown in the picture below.Includes most of Lux reulatory region, including the LuxR binding site which activates the right promoter. A putative LuxR autorepression binding site is also identified adjacent to the -10 site of the right promoter. This 2nd site has 55% identity with the first site. Putative inverted repeats (of size 18-27 bp) also exist between these two sites (not marked above), which may represent binding sites for other regulatory proteins. <img src="<bb_file>Image01.gif</bb_file>" width="614" height="362"> Unspecified.
mutable provenance: V. fischeri.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35
sequence annotation: LuxR/HSL
sequence annotation: BBa_R0063
sequence annotation: Putative LuxR/HSL
sequence annotation: start
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0063_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation2053
display id: annotation2053
version: 1
top level: lux pL
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0063/annotation2053/range2053/1

https://synbiohub.org/public/igem/BBa_R0063/annotation2053/range2053/1
type: Range
persistent identity: range2053
display id: range2053
version: 1
direction: direction/forward
top level: lux pL
start: 89
end: 94
orientation: inline

LuxR/HSL
type: SequenceAnnotation
persistent identity: annotation2051
display id: annotation2051
version: 1
top level: lux pL
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0063/annotation2051/range2051/1

https://synbiohub.org/public/igem/BBa_R0063/annotation2051/range2051/1
type: Range
persistent identity: range2051
display id: range2051
version: 1
direction: direction/forward
top level: lux pL
start: 1
end: 20
orientation: inline

BBa_R0063
type: SequenceAnnotation
persistent identity: annotation7071
display id: annotation7071
version: 1
top level: lux pL
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_R0063/annotation7071/range7071/1

https://synbiohub.org/public/igem/BBa_R0063/annotation7071/range7071/1
type: Range
persistent identity: range7071
display id: range7071
version: 1
direction: direction/forward
top level: lux pL
start: 1
end: 151
orientation: inline

Putative LuxR/HSL
type: SequenceAnnotation
persistent identity: annotation2055
display id: annotation2055
version: 1
top level: lux pL
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0063/annotation2055/range2055/1

https://synbiohub.org/public/igem/BBa_R0063/annotation2055/range2055/1
type: Range
persistent identity: range2055
display id: range2055
version: 1
direction: direction/forward
top level: lux pL
start: 130
end: 149
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2054
display id: annotation2054
version: 1
top level: lux pL
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_R0063/annotation2054/range2054/1

https://synbiohub.org/public/igem/BBa_R0063/annotation2054/range2054/1
type: Range
persistent identity: range2054
display id: range2054
version: 1
direction: direction/forward
top level: lux pL
start: 128
end: 128
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2052
display id: annotation2052
version: 1
top level: lux pL
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0063/annotation2052/range2052/1

https://synbiohub.org/public/igem/BBa_R0063/annotation2052/range2052/1
type: Range
persistent identity: range2052
display id: range2052
version: 1
direction: direction/forward
top level: lux pL
start: 115
end: 122
orientation: inline

BBa_K731520
type: ComponentDefinition
persistent identity: BBa K731520
display id: BBa_K731520
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: IPTG inducible GFPmut3b
creator: Francesco Guzzonato
created: 2012-08-21T11:00:00Z
modified: 2015-05-08T01:13:06Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _977_
m user id: 0
owner id: 12142
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This is a construct carrying the sfGFP gene with low-efficiency RBS, under lac operator control. lac repressor (encoded from lacI gene) is the regulator protein: in absence of lactose the protein binds to lac operator, preventing RNA-polymerase binding and transcription.Low, constitutive levels of transcription can be seen anyhow.
mutable provenance: This construct has ben obtained from ligation between BBa_K731500 and BBa_E0240.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_E0240
component: BBa_K731500
sequence annotation: BBa_E0240
sequence annotation: BBa_K731500
sequence: https://synbiohub.org/public/igem/BBa_K731520_sequence/1

BBa_E0240
type: Component
persistent identity: component2181566
display id: component2181566
version: 1
top level: BBa_K731520
access: public
definition: GFP report

BBa_K731500
type: Component
persistent identity: component2181555
display id: component2181555
version: 1
top level: BBa_K731520
access: public
definition: https://synbiohub.org/public/igem/BBa_K731500/1

BBa_E0240
type: SequenceAnnotation
persistent identity: annotation2181566
display id: annotation2181566
version: 1
top level: BBa_K731520
location: https://synbiohub.org/public/igem/BBa_K731520/annotation2181566/range2181566/1
component: BBa_E0240

https://synbiohub.org/public/igem/BBa_K731520/annotation2181566/range2181566/1
type: Range
persistent identity: range2181566
display id: range2181566
version: 1
direction: direction/reverse
top level: BBa_K731520
start: 1248
end: 2123
orientation: inline

BBa_K731500
type: SequenceAnnotation
persistent identity: annotation2181555
display id: annotation2181555
version: 1
top level: BBa_K731520
location: https://synbiohub.org/public/igem/BBa_K731520/annotation2181555/range2181555/1
component: BBa_K731500

https://synbiohub.org/public/igem/BBa_K731520/annotation2181555/range2181555/1
type: Range
persistent identity: range2181555
display id: range2181555
version: 1
direction: direction/reverse
top level: BBa_K731520
start: 1
end: 1239
orientation: inline

BBa_K733012
type: ComponentDefinition
persistent identity: BBa K733012
display id: BBa_K733012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: xylR+PxylA+RBS+ydcE+Ptms+RBS+ydcD: Cell Growth Inhibition Device
creator: WANG, Yuqi
created: 2012-09-15T11:00:00Z
modified: 2015-05-08T01:13:06Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _979_
m user id: 0
owner id: 12444
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Not available at this moment.
mutable notes: Not available at this moment.
mutable provenance: Not available at this moment.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K733010
component: BBa_K733011
sequence annotation: BBa_K733010
sequence annotation: BBa_K733011
sequence: https://synbiohub.org/public/igem/BBa_K733012_sequence/1

BBa_K733010
type: Component
persistent identity: component2183781
display id: component2183781
version: 1
top level: BBa_K733012
access: public
definition: https://synbiohub.org/public/igem/BBa_K733010/1

BBa_K733011
type: Component
persistent identity: component2183778
display id: component2183778
version: 1
top level: BBa_K733012
access: public
definition: https://synbiohub.org/public/igem/BBa_K733011/1

BBa_K733010
type: SequenceAnnotation
persistent identity: annotation2183781
display id: annotation2183781
version: 1
top level: BBa_K733012
location: https://synbiohub.org/public/igem/BBa_K733012/annotation2183781/range2183781/1
component: BBa_K733010

https://synbiohub.org/public/igem/BBa_K733012/annotation2183781/range2183781/1
type: Range
persistent identity: range2183781
display id: range2183781
version: 1
direction: direction/reverse
top level: BBa_K733012
start: 1773
end: 2146
orientation: inline

BBa_K733011
type: SequenceAnnotation
persistent identity: annotation2183778
display id: annotation2183778
version: 1
top level: BBa_K733012
location: https://synbiohub.org/public/igem/BBa_K733012/annotation2183778/range2183778/1
component: BBa_K733011

https://synbiohub.org/public/igem/BBa_K733012/annotation2183778/range2183778/1
type: Range
persistent identity: range2183778
display id: range2183778
version: 1
direction: direction/reverse
top level: BBa_K733012
start: 1
end: 1764
orientation: inline

BBa_K909007
type: ComponentDefinition
persistent identity: BBa K909007
display id: BBa_K909007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TetR DNA binding domain containing BamHI site for protein fusions
creator: Gintautas Vainorius
created: 2012-09-22T11:00:00Z
modified: 2015-05-08T01:13:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1174_
m user id: 0
owner id: 14094
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Truncated version of tetracycline repressor TetR, consisting of 1 ??? 127 amino acids, containing DNA binding domain. The rest of the structure, 8-10 helixes responsible for tetR dimerization, were removed in order to keep tetR-DBD in monomer form and prevent from efficient DNA binding and transcription repression. In addition, we introduced BamHI site at C terminus for an easy in tetR-DBD frame fusions with other proteins. Thus, it can be used in two hybrid systems for both, homo and hetero dimerizations, also one can use these fusions to turn protein-protein interaction into the repression of transcription.
mutable notes: Deletion of 8-10 helixes might lower the stability of protein, also TetR-DBD is no longer responsive to anhydrotetracycline
mutable provenance: E. coli Tn10 transposon
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: BamHI restriction site
sequence: https://synbiohub.org/public/igem/BBa_K909007_sequence/1

BamHI restriction site
type: SequenceAnnotation
persistent identity: annotation2194604
display id: annotation2194604
version: 1
top level: BBa_K909007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K909007/annotation2194604/range2194604/1

https://synbiohub.org/public/igem/BBa_K909007/annotation2194604/range2194604/1
type: Range
persistent identity: range2194604
display id: range2194604
version: 1
direction: direction/forward
top level: BBa_K909007
start: 382
end: 387
orientation: inline

BBa_K909005
type: ComponentDefinition
persistent identity: BBa K909005
display id: BBa_K909005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cI protein with EYFP as a reporter
creator: Deborah Huber
created: 2012-09-22T11:00:00Z
modified: 2015-05-08T01:13:44Z
discontinued: false
dominant: false
group u list: _1174_
m user id: 0
owner id: 14088
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: sdkuhdshkudak
mutable notes: dsasad
mutable provenance: sddas
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_E0430
component: BBa_C0051
component: BBa_B0034
sequence annotation: BBa_C0051
sequence annotation: BBa_B0034
sequence annotation: BBa_E0430
sequence: https://synbiohub.org/public/igem/BBa_K909005_sequence/1

BBa_E0430
type: Component
persistent identity: component2264574
display id: component2264574
version: 1
top level: BBa_K909005
access: public
definition: BBa_E0430

BBa_C0051
type: Component
persistent identity: component2264564
display id: component2264564
version: 1
top level: BBa_K909005
access: public
definition: cI lam

BBa_B0034
type: Component
persistent identity: component2264560
display id: component2264560
version: 1
top level: BBa_K909005
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_C0051
type: SequenceAnnotation
persistent identity: annotation2264564
display id: annotation2264564
version: 1
top level: BBa_K909005
location: https://synbiohub.org/public/igem/BBa_K909005/annotation2264564/range2264564/1
component: BBa_C0051

https://synbiohub.org/public/igem/BBa_K909005/annotation2264564/range2264564/1
type: Range
persistent identity: range2264564
display id: range2264564
version: 1
direction: direction/reverse
top level: BBa_K909005
start: 19
end: 793
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2264560
display id: annotation2264560
version: 1
top level: BBa_K909005
location: https://synbiohub.org/public/igem/BBa_K909005/annotation2264560/range2264560/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K909005/annotation2264560/range2264560/1
type: Range
persistent identity: range2264560
display id: range2264560
version: 1
direction: direction/reverse
top level: BBa_K909005
start: 1
end: 12
orientation: inline

BBa_E0430
type: SequenceAnnotation
persistent identity: annotation2264574
display id: annotation2264574
version: 1
top level: BBa_K909005
location: https://synbiohub.org/public/igem/BBa_K909005/annotation2264574/range2264574/1
component: BBa_E0430

https://synbiohub.org/public/igem/BBa_K909005/annotation2264574/range2264574/1
type: Range
persistent identity: range2264574
display id: range2264574
version: 1
direction: direction/reverse
top level: BBa_K909005
start: 802
end: 1679
orientation: inline

BBa_K936020
type: ComponentDefinition
persistent identity: BBa K936020
display id: BBa_K936020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: K206000+B0034+pelB+Cutinase+Polyhistidine Tag
creator: Mattan Hamou
created: 2012-09-19T11:00:00Z
modified: 2015-05-08T01:13:47Z
discontinued: false
dominant: false
group u list: _1201_
m user id: 0
owner id: 13899
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Full Cutinase Construct on psB1A3 backbone.
mutable notes: There's no terminator so it needs to be used with a backbone that ends with a 3
mutable provenance: http://www.google.com/url
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K936015
component: BBa_K936014
sequence annotation: BBa_K936015
sequence annotation: BBa_K936014
sequence: https://synbiohub.org/public/igem/BBa_K936020_sequence/1

BBa_K936015
type: Component
persistent identity: component2188710
display id: component2188710
version: 1
top level: BBa_K936020
access: public
definition: https://synbiohub.org/public/igem/BBa_K936015/1

BBa_K936014
type: Component
persistent identity: component2188714
display id: component2188714
version: 1
top level: BBa_K936020
access: public
definition: https://synbiohub.org/public/igem/BBa_K936014/1

BBa_K936015
type: SequenceAnnotation
persistent identity: annotation2188710
display id: annotation2188710
version: 1
top level: BBa_K936020
location: https://synbiohub.org/public/igem/BBa_K936020/annotation2188710/range2188710/1
component: BBa_K936015

https://synbiohub.org/public/igem/BBa_K936020/annotation2188710/range2188710/1
type: Range
persistent identity: range2188710
display id: range2188710
version: 1
direction: direction/reverse
top level: BBa_K936020
start: 1
end: 150
orientation: inline

BBa_K936014
type: SequenceAnnotation
persistent identity: annotation2188714
display id: annotation2188714
version: 1
top level: BBa_K936020
location: https://synbiohub.org/public/igem/BBa_K936020/annotation2188714/range2188714/1
component: BBa_K936014

https://synbiohub.org/public/igem/BBa_K936020/annotation2188714/range2188714/1
type: Range
persistent identity: range2188714
display id: range2188714
version: 1
direction: direction/reverse
top level: BBa_K936020
start: 157
end: 1125
orientation: inline

BBa_I746324
type: ComponentDefinition
persistent identity: BBa I746324
display id: BBa_I746324
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP under control of Psid promoter
creator: Stefan Milde
created: 2007-09-10T11:00:00Z
modified: 2015-08-31T04:08:04Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _116_
m user id: 0
owner id: 2122
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part is used to screen the level of induction of the Psid promoter (I746364). The Psid promoter is induced by a class of phage activators (P2 ogr: I746350, PSP3pag: I746351, phiR73 delta: I746352).
mutable notes: The Psid promoter was put upstream of I13504.
mutable provenance: registry (I13504) and own construct (I746364)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_E0040
component: BBa_B0010
component: BBa_I746364
component: BBa_B0034
component: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_I746364
sequence annotation: BBa_E0040
sequence: https://synbiohub.org/public/igem/BBa_I746324_sequence/1

BBa_E0040
type: Component
persistent identity: component1943842
display id: component1943842
version: 1
top level: BBa_I746324
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_B0010
type: Component
persistent identity: component1943843
display id: component1943843
version: 1
top level: BBa_I746324
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_I746364
type: Component
persistent identity: component1943838
display id: component1943838
version: 1
top level: BBa_I746324
access: public
definition: https://synbiohub.org/public/igem/BBa_I746364/1

BBa_B0034
type: Component
persistent identity: component1943840
display id: component1943840
version: 1
top level: BBa_I746324
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: Component
persistent identity: component1943845
display id: component1943845
version: 1
top level: BBa_I746324
access: public
definition: BBa_B0012

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1943840
display id: annotation1943840
version: 1
top level: BBa_I746324
location: https://synbiohub.org/public/igem/BBa_I746324/annotation1943840/range1943840/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I746324/annotation1943840/range1943840/1
type: Range
persistent identity: range1943840
display id: range1943840
version: 1
direction: direction/reverse
top level: BBa_I746324
start: 102
end: 113
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1943843
display id: annotation1943843
version: 1
top level: BBa_I746324
location: https://synbiohub.org/public/igem/BBa_I746324/annotation1943843/range1943843/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I746324/annotation1943843/range1943843/1
type: Range
persistent identity: range1943843
display id: range1943843
version: 1
direction: direction/forward
top level: BBa_I746324
start: 848
end: 927
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1943845
display id: annotation1943845
version: 1
top level: BBa_I746324
location: https://synbiohub.org/public/igem/BBa_I746324/annotation1943845/range1943845/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I746324/annotation1943845/range1943845/1
type: Range
persistent identity: range1943845
display id: range1943845
version: 1
direction: direction/forward
top level: BBa_I746324
start: 936
end: 976
orientation: inline

BBa_I746364
type: SequenceAnnotation
persistent identity: annotation1943838
display id: annotation1943838
version: 1
top level: BBa_I746324
location: https://synbiohub.org/public/igem/BBa_I746324/annotation1943838/range1943838/1
component: BBa_I746364

https://synbiohub.org/public/igem/BBa_I746324/annotation1943838/range1943838/1
type: Range
persistent identity: range1943838
display id: range1943838
version: 1
direction: direction/reverse
top level: BBa_I746324
start: 1
end: 93
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1943842
display id: annotation1943842
version: 1
top level: BBa_I746324
location: https://synbiohub.org/public/igem/BBa_I746324/annotation1943842/range1943842/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_I746324/annotation1943842/range1943842/1
type: Range
persistent identity: range1943842
display id: range1943842
version: 1
direction: direction/reverse
top level: BBa_I746324
start: 120
end: 839
orientation: inline

aiiA
type: ComponentDefinition
persistent identity: BBa C0160
display id: BBa_C0160
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: autoinducer inactivation enzyme aiiA (no LVA)
creator: jcbraff
created: 2004-05-26T11:00:00Z
modified: 2015-08-31T04:07:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 61
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: same as C0060 except no LVA tag
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: aiiA
sequence: https://synbiohub.org/public/igem/BBa_C0160_sequence/1

aiiA
type: SequenceAnnotation
persistent identity: annotation1891585
display id: annotation1891585
version: 1
top level: aiiA
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0160/annotation1891585/range1891585/1

https://synbiohub.org/public/igem/BBa_C0160/annotation1891585/range1891585/1
type: Range
persistent identity: range1891585
display id: range1891585
version: 1
direction: direction/forward
top level: aiiA
start: 1
end: 756
orientation: inline

BBa_K784013
type: ComponentDefinition
persistent identity: BBa K784013
display id: BBa_K784013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pLux+Theophylline riboswitch+mCherry
creator: Ilya Vainberg Slutskin
created: 2012-09-15T11:00:00Z
modified: 2015-05-08T01:13:21Z
discontinued: false
dominant: true
group u list: _1039_
m user id: 0
owner id: 11677
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This sequence is the product of restriction cloning of the theophylline riboswitch downstream to the pLux promoter. In the presence of LuxR transcription can be induced by 3OC6HSL.
mutable notes: No design considerations.
mutable provenance: Cloning of theophylline riboswitch downstream to the pLux promoter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K784006
component: BBa_R0062
sequence annotation: BBa_R0062
sequence annotation: BBa_K784006
sequence: https://synbiohub.org/public/igem/BBa_K784013_sequence/1

BBa_K784006
type: Component
persistent identity: component2183694
display id: component2183694
version: 1
top level: BBa_K784013
access: public
definition: BBa_K784006

BBa_R0062
type: Component
persistent identity: component2183682
display id: component2183682
version: 1
top level: BBa_K784013
access: public
definition: lux pR

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation2183682
display id: annotation2183682
version: 1
top level: BBa_K784013
location: https://synbiohub.org/public/igem/BBa_K784013/annotation2183682/range2183682/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_K784013/annotation2183682/range2183682/1
type: Range
persistent identity: range2183682
display id: range2183682
version: 1
direction: direction/forward
top level: BBa_K784013
start: 1
end: 55
orientation: inline

BBa_K784006
type: SequenceAnnotation
persistent identity: annotation2183694
display id: annotation2183694
version: 1
top level: BBa_K784013
location: https://synbiohub.org/public/igem/BBa_K784013/annotation2183694/range2183694/1
component: BBa_K784006

https://synbiohub.org/public/igem/BBa_K784013/annotation2183694/range2183694/1
type: Range
persistent identity: range2183694
display id: range2183694
version: 1
direction: direction/reverse
top level: BBa_K784013
start: 64
end: 851
orientation: inline

BBa_S05055
type: ComponentDefinition
persistent identity: BBa S05055
display id: BBa_S05055
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: R0010:P0451
creator: Muyuan Chen
created: 2012-09-17T11:00:00Z
modified: 2015-05-08T01:14:49Z
discontinued: false
dominant: false
group u list: _9_
m user id: 0
owner id: 12559
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_R0010
component: BBa_P0451
sequence annotation: BBa_R0010
sequence annotation: BBa_P0451
sequence: https://synbiohub.org/public/igem/BBa_S05055_sequence/1

BBa_R0010
type: Component
persistent identity: component2184443
display id: component2184443
version: 1
top level: BBa_S05055
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_P0451
type: Component
persistent identity: component2184462
display id: component2184462
version: 1
top level: BBa_S05055
access: public
definition: https://synbiohub.org/public/igem/BBa_P0451/1

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2184443
display id: annotation2184443
version: 1
top level: BBa_S05055
location: https://synbiohub.org/public/igem/BBa_S05055/annotation2184443/range2184443/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_S05055/annotation2184443/range2184443/1
type: Range
persistent identity: range2184443
display id: range2184443
version: 1
direction: direction/forward
top level: BBa_S05055
start: 1
end: 200
orientation: inline

BBa_P0451
type: SequenceAnnotation
persistent identity: annotation2184462
display id: annotation2184462
version: 1
top level: BBa_S05055
location: https://synbiohub.org/public/igem/BBa_S05055/annotation2184462/range2184462/1
component: BBa_P0451

https://synbiohub.org/public/igem/BBa_S05055/annotation2184462/range2184462/1
type: Range
persistent identity: range2184462
display id: range2184462
version: 1
direction: direction/reverse
top level: BBa_S05055
start: 209
end: 1138
orientation: inline

BBa_K784047
type: ComponentDefinition
persistent identity: BBa K784047
display id: BBa_K784047
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Theophylline riboswitch + wt T7 RNAP
creator: Asaf Blumental
created: 2012-09-25T11:00:00Z
modified: 2015-05-08T01:13:22Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1039_
m user id: 0
owner id: 11677
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This part contains an engineered theophylline riboswitch (RS) ([http://partsregistry.org/Part:BBa_K784005 BBa K784005]) and T7 RNA polymerase ([http://partsregistry.org/Part:BBa_I2032 BBa I2032]). The riboswitch contains a RBS and it will be exposed and starts translating the T7 RNAP only after inserting of theophylline. The RBS and the start codon of the gene are part of the riboswitch sequence and are responsible for its structure, so they are not to be changed. This part was constructed using fusion PCR, so the RS and the T7 RNAP are adjacent to one another without any restriction site between them. There is an additional stop codon at the end of the gene.
mutable notes: The riboswitch had to fused to the RNAP using assembly PCR.
mutable provenance: Assembly PCR of [http://partsregistry.org/Part:BBa_K784005 BBa K784005] and [http://partsregistry.org/Part:BBa_I2032 BBa_I2032]. [http://partsregistry.org/Part:BBa_I2032 BBa I2032] was received from Ann K. Ganesan.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K784005
component: BBa_I2032
sequence annotation: BBa_I2032
sequence annotation: BBa_K784005
sequence: https://synbiohub.org/public/igem/BBa_K784047_sequence/1

BBa_K784005
type: Component
persistent identity: component2199519
display id: component2199519
version: 1
top level: BBa_K784047
access: public
definition: https://synbiohub.org/public/igem/BBa_K784005/1

BBa_I2032
type: Component
persistent identity: component2199521
display id: component2199521
version: 1
top level: BBa_K784047
access: public
definition: https://synbiohub.org/public/igem/BBa_I2032/1

BBa_I2032
type: SequenceAnnotation
persistent identity: annotation2199521
display id: annotation2199521
version: 1
top level: BBa_K784047
location: https://synbiohub.org/public/igem/BBa_K784047/annotation2199521/range2199521/1
component: BBa_I2032

https://synbiohub.org/public/igem/BBa_K784047/annotation2199521/range2199521/1
type: Range
persistent identity: range2199521
display id: range2199521
version: 1
direction: direction/reverse
top level: BBa_K784047
start: 75
end: 2729
orientation: inline

BBa_K784005
type: SequenceAnnotation
persistent identity: annotation2199519
display id: annotation2199519
version: 1
top level: BBa_K784047
location: https://synbiohub.org/public/igem/BBa_K784047/annotation2199519/range2199519/1
component: BBa_K784005

https://synbiohub.org/public/igem/BBa_K784047/annotation2199519/range2199519/1
type: Range
persistent identity: range2199519
display id: range2199519
version: 1
direction: direction/reverse
top level: BBa_K784047
start: 1
end: 74
orientation: inline

BBa_I716211
type: ComponentDefinition
persistent identity: BBa I716211
display id: BBa_I716211
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Barnase active peptide
creator: Samantha Liang
created: 2007-06-04T11:00:00Z
modified: 2015-08-31T04:07:51Z
discontinued: false
dominant: true
experience: experience/None
group u list: _110_
m user id: 0
owner id: 933
owning group id: 52
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Barnase active peptide (no start codon)
mutable notes: PCR sil03F and sil03R on barnase region (348 bp, BglII/XhoI) Sub into pBca9145-Bca1089 (BglII/XhoI, 2063+696, L) Product is pBca9145-I716211 sil03F Forward BglII biobrick of barnase ccaaaAGATCTgcacaggttatcaacacgtttg sil03R Reverse XholI/BamHI biobrick of barnase gctagCTCGAGttaGGATCCttatctgatttttgtaaagg
mutable provenance: PCR sil03F and sil03R on barnase region (348 bp, BglII/XhoI) Sub into pBca9145-Bca1089 (BglII/XhoI, 2063+696, L) Product is pBca9145-I716211 sil03F Forward BglII biobrick of barnase ccaaaAGATCTgcacaggttatcaacacgtttg sil03R Reverse XholI/BamHI biobrick of barnase gctagCTCGAGttaGGATCCttatctgatttttgtaaagg
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_I716211_sequence/1

BBa_I6102
type: ComponentDefinition
persistent identity: BBa I6102
display id: BBa_I6102
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter O_H Test R0073.E0430
creator: Randy Rettberg
created: 2004-04-21T11:00:00Z
modified: 2015-08-31T04:07:44Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_E0030
component: BBa_R0073
component: BBa_B0012
component: BBa_B0010
component: BBa_B0034
sequence annotation: BBa_E0030
sequence annotation: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_B0010
sequence annotation: BBa_R0073
sequence: https://synbiohub.org/public/igem/BBa_I6102_sequence/1

BBa_E0030
type: Component
persistent identity: component949754
display id: component949754
version: 1
top level: BBa_I6102
access: public
definition: https://synbiohub.org/public/igem/BBa_E0030/1

BBa_R0073
type: Component
persistent identity: component949747
display id: component949747
version: 1
top level: BBa_I6102
access: public
definition: https://synbiohub.org/public/igem/BBa_R0073/1

BBa_B0012
type: Component
persistent identity: component949769
display id: component949769
version: 1
top level: BBa_I6102
access: public
definition: BBa_B0012

BBa_B0010
type: Component
persistent identity: component949759
display id: component949759
version: 1
top level: BBa_I6102
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component949752
display id: component949752
version: 1
top level: BBa_I6102
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation949754
display id: annotation949754
version: 1
top level: BBa_I6102
location: https://synbiohub.org/public/igem/BBa_I6102/annotation949754/range949754/1
component: BBa_E0030

https://synbiohub.org/public/igem/BBa_I6102/annotation949754/range949754/1
type: Range
persistent identity: range949754
display id: range949754
version: 1
direction: direction/forward
top level: BBa_I6102
start: 94
end: 816
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation949769
display id: annotation949769
version: 1
top level: BBa_I6102
location: https://synbiohub.org/public/igem/BBa_I6102/annotation949769/range949769/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I6102/annotation949769/range949769/1
type: Range
persistent identity: range949769
display id: range949769
version: 1
direction: direction/forward
top level: BBa_I6102
start: 913
end: 953
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation949752
display id: annotation949752
version: 1
top level: BBa_I6102
location: https://synbiohub.org/public/igem/BBa_I6102/annotation949752/range949752/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I6102/annotation949752/range949752/1
type: Range
persistent identity: range949752
display id: range949752
version: 1
direction: direction/forward
top level: BBa_I6102
start: 76
end: 87
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation949759
display id: annotation949759
version: 1
top level: BBa_I6102
location: https://synbiohub.org/public/igem/BBa_I6102/annotation949759/range949759/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I6102/annotation949759/range949759/1
type: Range
persistent identity: range949759
display id: range949759
version: 1
direction: direction/forward
top level: BBa_I6102
start: 825
end: 904
orientation: inline

BBa_R0073
type: SequenceAnnotation
persistent identity: annotation949747
display id: annotation949747
version: 1
top level: BBa_I6102
location: https://synbiohub.org/public/igem/BBa_I6102/annotation949747/range949747/1
component: BBa_R0073

https://synbiohub.org/public/igem/BBa_I6102/annotation949747/range949747/1
type: Range
persistent identity: range949747
display id: range949747
version: 1
direction: direction/forward
top level: BBa_I6102
start: 1
end: 67
orientation: inline

TD1.0
type: ComponentDefinition
persistent identity: BBa K750009
display id: BBa_K750009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TIME DELAY1.0:LuxI(RBS1.0)->LuxR->LuxPR->GFP
creator: Sifan Wang,Ruosang Qiu,Xinyi Yao,Qingshu Wu
created: 2012-09-18T11:00:00Z
modified: 2015-05-08T01:13:12Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1001_
m user id: 0
owner id: 14212
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This part contains BBa-K750001 and BBa_K750007. Arabinose will activate the promoter Pbad, which will cause production of protein LuxI and LuxR. The protein LuxI, LuxR and LuxPR make up the quorum sensing system, which leads to the expression of GFP.
mutable notes: editing
mutable provenance: We built this part by biobricks from the DNA distribution kit plates 2012.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_K750001
component: BBa_K750007
sequence annotation: BBa_K750001
sequence annotation: BBa_K750007
sequence: https://synbiohub.org/public/igem/BBa_K750009_sequence/1

BBa_K750001
type: Component
persistent identity: component2312609
display id: component2312609
version: 1
top level: TD1.0
access: public
definition: https://synbiohub.org/public/igem/BBa_K750001/1

BBa_K750007
type: Component
persistent identity: component2312646
display id: component2312646
version: 1
top level: TD1.0
access: public
definition: https://synbiohub.org/public/igem/BBa_K750007/1

BBa_K750001
type: SequenceAnnotation
persistent identity: annotation2312609
display id: annotation2312609
version: 1
top level: TD1.0
location: https://synbiohub.org/public/igem/BBa_K750009/annotation2312609/range2312609/1
component: BBa_K750001

https://synbiohub.org/public/igem/BBa_K750009/annotation2312609/range2312609/1
type: Range
persistent identity: range2312609
display id: range2312609
version: 1
direction: direction/reverse
top level: TD1.0
start: 1
end: 936
orientation: inline

BBa_K750007
type: SequenceAnnotation
persistent identity: annotation2312646
display id: annotation2312646
version: 1
top level: TD1.0
location: https://synbiohub.org/public/igem/BBa_K750009/annotation2312646/range2312646/1
component: BBa_K750007

https://synbiohub.org/public/igem/BBa_K750009/annotation2312646/range2312646/1
type: Range
persistent identity: range2312646
display id: range2312646
version: 1
direction: direction/reverse
top level: TD1.0
start: 945
end: 2964
orientation: inline

BBa_K876057
type: ComponentDefinition
persistent identity: BBa K876057
display id: BBa_K876057
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pTetR-lasR-Term-Term
creator: Leah Ferrell, Sanket Shah, Shayan Ghassemi, Zakaraya Ashour
created: 2012-10-01T11:00:00Z
modified: 2015-05-08T01:13:39Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1138_
m user id: 0
owner id: 13835
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: pTetR-lasR-TT
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_C0079
component: BBa_R0040
component: BBa_B0015
sequence annotation: BBa_C0079
sequence annotation: BBa_B0015
sequence annotation: BBa_R0040
sequence: https://synbiohub.org/public/igem/BBa_K876057_sequence/1

BBa_C0079
type: Component
persistent identity: component2251286
display id: component2251286
version: 1
top level: BBa_K876057
access: public
definition: https://synbiohub.org/public/igem/BBa_C0079/1

BBa_R0040
type: Component
persistent identity: component2251277
display id: component2251277
version: 1
top level: BBa_K876057
access: public
definition: p(tetR)

BBa_B0015
type: Component
persistent identity: component2251293
display id: component2251293
version: 1
top level: BBa_K876057
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_C0079
type: SequenceAnnotation
persistent identity: annotation2251286
display id: annotation2251286
version: 1
top level: BBa_K876057
location: https://synbiohub.org/public/igem/BBa_K876057/annotation2251286/range2251286/1
component: BBa_C0079

https://synbiohub.org/public/igem/BBa_K876057/annotation2251286/range2251286/1
type: Range
persistent identity: range2251286
display id: range2251286
version: 1
direction: direction/reverse
top level: BBa_K876057
start: 61
end: 841
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2251293
display id: annotation2251293
version: 1
top level: BBa_K876057
location: https://synbiohub.org/public/igem/BBa_K876057/annotation2251293/range2251293/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K876057/annotation2251293/range2251293/1
type: Range
persistent identity: range2251293
display id: range2251293
version: 1
direction: direction/reverse
top level: BBa_K876057
start: 850
end: 978
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2251277
display id: annotation2251277
version: 1
top level: BBa_K876057
location: https://synbiohub.org/public/igem/BBa_K876057/annotation2251277/range2251277/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K876057/annotation2251277/range2251277/1
type: Range
persistent identity: range2251277
display id: range2251277
version: 1
direction: direction/forward
top level: BBa_K876057
start: 1
end: 54
orientation: inline

[pTET][loc
type: ComponentDefinition
persistent identity: BBa J01060
display id: BBa_J01060
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: OnLock1 = [pTet][Lock1]
creator: Golden Bear
created: 2005-11-05T12:00:00Z
modified: 2015-08-31T04:08:12Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _13_
m user id: 0
owner id: 395
owning group id: 13
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: [pTet][Lock1]
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_R0040
component: BBa_J01010
sequence annotation: BBa_R0040
sequence annotation: BBa_J01010
sequence: https://synbiohub.org/public/igem/BBa_J01060_sequence/1

BBa_R0040
type: Component
persistent identity: component1738591
display id: component1738591
version: 1
top level: [pTET][loc
access: public
definition: p(tetR)

BBa_J01010
type: Component
persistent identity: component1738596
display id: component1738596
version: 1
top level: [pTET][loc
access: public
definition: https://synbiohub.org/public/igem/BBa_J01010/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1738591
display id: annotation1738591
version: 1
top level: [pTET][loc
location: https://synbiohub.org/public/igem/BBa_J01060/annotation1738591/range1738591/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_J01060/annotation1738591/range1738591/1
type: Range
persistent identity: range1738591
display id: range1738591
version: 1
direction: direction/forward
top level: [pTET][loc
start: 1
end: 54
orientation: inline

BBa_J01010
type: SequenceAnnotation
persistent identity: annotation1738596
display id: annotation1738596
version: 1
top level: [pTET][loc
location: https://synbiohub.org/public/igem/BBa_J01060/annotation1738596/range1738596/1
component: BBa_J01010

https://synbiohub.org/public/igem/BBa_J01060/annotation1738596/range1738596/1
type: Range
persistent identity: range1738596
display id: range1738596
version: 1
direction: direction/forward
top level: [pTET][loc
start: 63
end: 102
orientation: inline

BBa_I714033
type: ComponentDefinition
persistent identity: BBa I714033
display id: BBa_I714033
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: crRNA-lock3-var1 (A new "lock" designed by our team)
creator: Mingzhi Qu
created: 2007-10-21T11:00:00Z
modified: 2015-08-31T04:07:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _142_
m user id: 0
owner id: 2248
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: crRNA(I714033) and taRNA(I714034) whose structure resemble that of J23078 (lock3) and J23066 (key3) but with a different matching regions to minimize the possible interaction with the key3-lock3.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence: https://synbiohub.org/public/igem/BBa_I714033_sequence/1

GFP-AID
type: ComponentDefinition
persistent identity: BBa K812010
display id: BBa_K812010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP fused with AID-tag (ubuquitinase E3 OsTirI recoginition domain)
creator: Cyrille Pauthenier
created: 2012-09-17T11:00:00Z
modified: 2015-05-08T01:13:27Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _1069_
m user id: 0
owner id: 8998
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part is a super folded GFP fused to an AID tag for its recignition by the ubiquitinase E3 that induces its degradation in the presence of auxin in the eukaryote cell. This part is a part of a device pattented by Kanemaki Masato, Kakimoto Tatsuo, Nishimura Kohei, Takisawa Haruhiko and Fukagawa Tatsuo for yeast and mammalian cells use (http://www.freepatentsonline.com/y2012/0115232.html). However the pattent does not cover the use for oviparian such as frogs and chicken. The GFP-AID contains 2 NLS domains that relocate the protein in the nucleus of the cells for a better signal concentration.
mutable notes: This part has been biobricked using PCR method and cloned in pSB1C3
mutable provenance: This part have been extracted by PCR from the plasmid pNHK60 obtained from the riken registry.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: NLS2
sequence annotation: NLS1
sequence annotation: Kozac
sequence annotation: GFP
sequence annotation: unfolded linker
sequence annotation: AID degradation tag
sequence: https://synbiohub.org/public/igem/BBa_K812010_sequence/1

NLS2
type: SequenceAnnotation
persistent identity: annotation2184374
display id: annotation2184374
version: 1
top level: GFP-AID
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K812010/annotation2184374/range2184374/1

https://synbiohub.org/public/igem/BBa_K812010/annotation2184374/range2184374/1
type: Range
persistent identity: range2184374
display id: range2184374
version: 1
direction: direction/forward
top level: GFP-AID
start: 1480
end: 1500
orientation: inline

NLS1
type: SequenceAnnotation
persistent identity: annotation2184373
display id: annotation2184373
version: 1
top level: GFP-AID
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K812010/annotation2184373/range2184373/1

https://synbiohub.org/public/igem/BBa_K812010/annotation2184373/range2184373/1
type: Range
persistent identity: range2184373
display id: range2184373
version: 1
direction: direction/forward
top level: GFP-AID
start: 1459
end: 1479
orientation: inline

Kozac
type: SequenceAnnotation
persistent identity: annotation2184369
display id: annotation2184369
version: 1
top level: GFP-AID
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K812010/annotation2184369/range2184369/1

https://synbiohub.org/public/igem/BBa_K812010/annotation2184369/range2184369/1
type: Range
persistent identity: range2184369
display id: range2184369
version: 1
direction: direction/forward
top level: GFP-AID
start: 1
end: 6
orientation: inline

GFP
type: SequenceAnnotation
persistent identity: annotation2184370
display id: annotation2184370
version: 1
top level: GFP-AID
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K812010/annotation2184370/range2184370/1

https://synbiohub.org/public/igem/BBa_K812010/annotation2184370/range2184370/1
type: Range
persistent identity: range2184370
display id: range2184370
version: 1
direction: direction/forward
top level: GFP-AID
start: 7
end: 723
orientation: inline

unfolded linker
type: SequenceAnnotation
persistent identity: annotation2184371
display id: annotation2184371
version: 1
top level: GFP-AID
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K812010/annotation2184371/range2184371/1

https://synbiohub.org/public/igem/BBa_K812010/annotation2184371/range2184371/1
type: Range
persistent identity: range2184371
display id: range2184371
version: 1
direction: direction/forward
top level: GFP-AID
start: 724
end: 756
orientation: inline

AID degradation tag
type: SequenceAnnotation
persistent identity: annotation2184372
display id: annotation2184372
version: 1
top level: GFP-AID
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K812010/annotation2184372/range2184372/1

https://synbiohub.org/public/igem/BBa_K812010/annotation2184372/range2184372/1
type: Range
persistent identity: range2184372
display id: range2184372
version: 1
direction: direction/forward
top level: GFP-AID
start: 757
end: 1443
orientation: inline

BBa_I13528
type: ComponentDefinition
persistent identity: BBa I13528
display id: BBa_I13528
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Screening plasmid v1.0 intermediate
creator: Josh Michener
created: 2005-07-28T11:00:00Z
modified: 2015-08-31T04:07:35Z
discontinued: false
dominant: true
experience: experience/None
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Intermediate in screening plasmid v2 construction.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_I13507
component: BBa_I13457
sequence annotation: BBa_I13507
sequence annotation: BBa_I13457
sequence: https://synbiohub.org/public/igem/BBa_I13528_sequence/1

BBa_I13507
type: Component
persistent identity: component2251425
display id: component2251425
version: 1
top level: BBa_I13528
access: public
definition: https://synbiohub.org/public/igem/BBa_I13507/1

BBa_I13457
type: Component
persistent identity: component2251413
display id: component2251413
version: 1
top level: BBa_I13528
access: public
definition: BBa_I13457

BBa_I13507
type: SequenceAnnotation
persistent identity: annotation2251425
display id: annotation2251425
version: 1
top level: BBa_I13528
location: https://synbiohub.org/public/igem/BBa_I13528/annotation2251425/range2251425/1
component: BBa_I13507

https://synbiohub.org/public/igem/BBa_I13528/annotation2251425/range2251425/1
type: Range
persistent identity: range2251425
display id: range2251425
version: 1
direction: direction/reverse
top level: BBa_I13528
start: 120
end: 980
orientation: inline

BBa_I13457
type: SequenceAnnotation
persistent identity: annotation2251413
display id: annotation2251413
version: 1
top level: BBa_I13528
location: https://synbiohub.org/public/igem/BBa_I13528/annotation2251413/range2251413/1
component: BBa_I13457

https://synbiohub.org/public/igem/BBa_I13528/annotation2251413/range2251413/1
type: Range
persistent identity: range2251413
display id: range2251413
version: 1
direction: direction/reverse
top level: BBa_I13528
start: 1
end: 111
orientation: inline

BBa_I718014
type: ComponentDefinition
persistent identity: BBa I718014
display id: BBa_I718014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: rbs-dapA (B. Subtilis)
creator: David Bikard
created: 2007-10-25T11:00:00Z
modified: 2015-08-31T04:07:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _141_
m user id: 0
owner id: 1481
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: none
mutable notes: none
mutable provenance: none
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_I718005
component: BBa_B0030
sequence annotation: BBa_B0030
sequence annotation: BBa_I718005
sequence: https://synbiohub.org/public/igem/BBa_I718014_sequence/1

BBa_I718005
type: Component
persistent identity: component1956197
display id: component1956197
version: 1
top level: BBa_I718014
access: public
definition: https://synbiohub.org/public/igem/BBa_I718005/1

BBa_B0030
type: Component
persistent identity: component1956192
display id: component1956192
version: 1
top level: BBa_I718014
access: public
definition: BBa_B0030

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1956192
display id: annotation1956192
version: 1
top level: BBa_I718014
location: https://synbiohub.org/public/igem/BBa_I718014/annotation1956192/range1956192/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_I718014/annotation1956192/range1956192/1
type: Range
persistent identity: range1956192
display id: range1956192
version: 1
direction: direction/forward
top level: BBa_I718014
start: 1
end: 15
orientation: inline

BBa_I718005
type: SequenceAnnotation
persistent identity: annotation1956197
display id: annotation1956197
version: 1
top level: BBa_I718014
location: https://synbiohub.org/public/igem/BBa_I718014/annotation1956197/range1956197/1
component: BBa_I718005

https://synbiohub.org/public/igem/BBa_I718014/annotation1956197/range1956197/1
type: Range
persistent identity: range1956197
display id: range1956197
version: 1
direction: direction/reverse
top level: BBa_I718014
start: 22
end: 897
orientation: inline

RFP1
type: ComponentDefinition
persistent identity: BBa I715022
display id: BBa_I715022
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Amino Portion of RFP
creator: Andrew Martens
created: 2007-06-24T11:00:00Z
modified: 2016-01-25T04:43:58Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _120_
m user id: 4206
owner id: 201
owning group id: 61
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: af
mutable notes: afd
mutable provenance: adf
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: start
sequence annotation: Coding sequence
sequence: https://synbiohub.org/public/igem/BBa_I715022_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation1935380
display id: annotation1935380
version: 1
top level: RFP1
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_I715022/annotation1935380/range1935380/1

https://synbiohub.org/public/igem/BBa_I715022/annotation1935380/range1935380/1
type: Range
persistent identity: range1935380
display id: range1935380
version: 1
direction: direction/forward
top level: RFP1
start: 1
end: 3
orientation: inline

Coding sequence
type: SequenceAnnotation
persistent identity: annotation1935381
display id: annotation1935381
version: 1
top level: RFP1
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I715022/annotation1935381/range1935381/1

https://synbiohub.org/public/igem/BBa_I715022/annotation1935381/range1935381/1
type: Range
persistent identity: range1935381
display id: range1935381
version: 1
direction: direction/forward
top level: RFP1
start: 1
end: 462
orientation: inline

BBa_I6103
type: ComponentDefinition
persistent identity: BBa I6103
display id: BBa_I6103
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter O_H Test R0074.E0430
creator: Randy Rettberg
created: 2004-04-21T11:00:00Z
modified: 2015-08-31T04:07:44Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_E0030
component: BBa_B0012
component: BBa_B0010
component: BBa_R0074
component: BBa_B0034
sequence annotation: BBa_B0010
sequence annotation: BBa_E0030
sequence annotation: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_R0074
sequence: https://synbiohub.org/public/igem/BBa_I6103_sequence/1

BBa_E0030
type: Component
persistent identity: component949790
display id: component949790
version: 1
top level: BBa_I6103
access: public
definition: https://synbiohub.org/public/igem/BBa_E0030/1

BBa_B0012
type: Component
persistent identity: component949805
display id: component949805
version: 1
top level: BBa_I6103
access: public
definition: BBa_B0012

BBa_B0010
type: Component
persistent identity: component949795
display id: component949795
version: 1
top level: BBa_I6103
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_R0074
type: Component
persistent identity: component949783
display id: component949783
version: 1
top level: BBa_I6103
access: public
definition: PenI

BBa_B0034
type: Component
persistent identity: component949788
display id: component949788
version: 1
top level: BBa_I6103
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation949795
display id: annotation949795
version: 1
top level: BBa_I6103
location: https://synbiohub.org/public/igem/BBa_I6103/annotation949795/range949795/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I6103/annotation949795/range949795/1
type: Range
persistent identity: range949795
display id: range949795
version: 1
direction: direction/forward
top level: BBa_I6103
start: 835
end: 914
orientation: inline

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation949790
display id: annotation949790
version: 1
top level: BBa_I6103
location: https://synbiohub.org/public/igem/BBa_I6103/annotation949790/range949790/1
component: BBa_E0030

https://synbiohub.org/public/igem/BBa_I6103/annotation949790/range949790/1
type: Range
persistent identity: range949790
display id: range949790
version: 1
direction: direction/forward
top level: BBa_I6103
start: 104
end: 826
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation949805
display id: annotation949805
version: 1
top level: BBa_I6103
location: https://synbiohub.org/public/igem/BBa_I6103/annotation949805/range949805/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I6103/annotation949805/range949805/1
type: Range
persistent identity: range949805
display id: range949805
version: 1
direction: direction/forward
top level: BBa_I6103
start: 923
end: 963
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation949788
display id: annotation949788
version: 1
top level: BBa_I6103
location: https://synbiohub.org/public/igem/BBa_I6103/annotation949788/range949788/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I6103/annotation949788/range949788/1
type: Range
persistent identity: range949788
display id: range949788
version: 1
direction: direction/forward
top level: BBa_I6103
start: 86
end: 97
orientation: inline

BBa_R0074
type: SequenceAnnotation
persistent identity: annotation949783
display id: annotation949783
version: 1
top level: BBa_I6103
location: https://synbiohub.org/public/igem/BBa_I6103/annotation949783/range949783/1
component: BBa_R0074

https://synbiohub.org/public/igem/BBa_I6103/annotation949783/range949783/1
type: Range
persistent identity: range949783
display id: range949783
version: 1
direction: direction/forward
top level: BBa_I6103
start: 1
end: 77
orientation: inline

BBa_K844008
type: ComponentDefinition
persistent identity: BBa K844008
display id: BBa_K844008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Spider Silk 1x Subunit "B" (Balanced tRNA codon optimized) with Met (ATG) added
creator: Andrea Halling
created: 2012-10-01T11:00:00Z
modified: 2016-02-10T11:28:32Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1104_
m user id: 4206
owner id: 13876
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Spider silk subunit optimized to use a reduced set of tRNA codons (1 or 2 codons per amino acid); contains two elasticity domains and one strength domain, also contains 5??? Met (ATG) codon.
mutable notes: none
mutable provenance: Created using mutagenesis PCR to add upstream Met (ATG) to part BBa_K844004
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Beta-spiral
sequence annotation: Beta-sprial
sequence annotation: Strength Domain
sequence annotation: Start
sequence annotation: Beta-helix
sequence annotation: Elasticity Domain
sequence annotation: Linker Domain
sequence: https://synbiohub.org/public/igem/BBa_K844008_sequence/1

Beta-spiral
type: SequenceAnnotation
persistent identity: annotation2212456
display id: annotation2212456
version: 1
top level: BBa_K844008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K844008/annotation2212456/range2212456/1

https://synbiohub.org/public/igem/BBa_K844008/annotation2212456/range2212456/1
type: Range
persistent identity: range2212456
display id: range2212456
version: 1
direction: direction/forward
top level: BBa_K844008
start: 49
end: 63
orientation: inline

Beta-spiral
type: SequenceAnnotation
persistent identity: annotation2212460
display id: annotation2212460
version: 1
top level: BBa_K844008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K844008/annotation2212460/range2212460/1

https://synbiohub.org/public/igem/BBa_K844008/annotation2212460/range2212460/1
type: Range
persistent identity: range2212460
display id: range2212460
version: 1
direction: direction/forward
top level: BBa_K844008
start: 98
end: 113
orientation: inline

Beta-spiral
type: SequenceAnnotation
persistent identity: annotation2212462
display id: annotation2212462
version: 1
top level: BBa_K844008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K844008/annotation2212462/range2212462/1

https://synbiohub.org/public/igem/BBa_K844008/annotation2212462/range2212462/1
type: Range
persistent identity: range2212462
display id: range2212462
version: 1
direction: direction/forward
top level: BBa_K844008
start: 133
end: 147
orientation: inline

Beta-spiral
type: SequenceAnnotation
persistent identity: annotation2212455
display id: annotation2212455
version: 1
top level: BBa_K844008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K844008/annotation2212455/range2212455/1

https://synbiohub.org/public/igem/BBa_K844008/annotation2212455/range2212455/1
type: Range
persistent identity: range2212455
display id: range2212455
version: 1
direction: direction/forward
top level: BBa_K844008
start: 34
end: 48
orientation: inline

Beta-spiral
type: SequenceAnnotation
persistent identity: annotation2212463
display id: annotation2212463
version: 1
top level: BBa_K844008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K844008/annotation2212463/range2212463/1

https://synbiohub.org/public/igem/BBa_K844008/annotation2212463/range2212463/1
type: Range
persistent identity: range2212463
display id: range2212463
version: 1
direction: direction/forward
top level: BBa_K844008
start: 156
end: 171
orientation: inline

Beta-spiral
type: SequenceAnnotation
persistent identity: annotation2212454
display id: annotation2212454
version: 1
top level: BBa_K844008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K844008/annotation2212454/range2212454/1

https://synbiohub.org/public/igem/BBa_K844008/annotation2212454/range2212454/1
type: Range
persistent identity: range2212454
display id: range2212454
version: 1
direction: direction/forward
top level: BBa_K844008
start: 13
end: 27
orientation: inline

Beta-sprial
type: SequenceAnnotation
persistent identity: annotation2212461
display id: annotation2212461
version: 1
top level: BBa_K844008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K844008/annotation2212461/range2212461/1

https://synbiohub.org/public/igem/BBa_K844008/annotation2212461/range2212461/1
type: Range
persistent identity: range2212461
display id: range2212461
version: 1
direction: direction/forward
top level: BBa_K844008
start: 117
end: 132
orientation: inline

Strength Domain
type: SequenceAnnotation
persistent identity: annotation2212465
display id: annotation2212465
version: 1
top level: BBa_K844008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K844008/annotation2212465/range2212465/1

https://synbiohub.org/public/igem/BBa_K844008/annotation2212465/range2212465/1
type: Range
persistent identity: range2212465
display id: range2212465
version: 1
direction: direction/forward
top level: BBa_K844008
start: 189
end: 207
orientation: inline

Start
type: SequenceAnnotation
persistent identity: annotation2212451
display id: annotation2212451
version: 1
top level: BBa_K844008
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K844008/annotation2212451/range2212451/1

https://synbiohub.org/public/igem/BBa_K844008/annotation2212451/range2212451/1
type: Range
persistent identity: range2212451
display id: range2212451
version: 1
direction: direction/forward
top level: BBa_K844008
start: 1
end: 3
orientation: inline

Beta-helix
type: SequenceAnnotation
persistent identity: annotation2212452
display id: annotation2212452
version: 1
top level: BBa_K844008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K844008/annotation2212452/range2212452/1

https://synbiohub.org/public/igem/BBa_K844008/annotation2212452/range2212452/1
type: Range
persistent identity: range2212452
display id: range2212452
version: 1
direction: direction/forward
top level: BBa_K844008
start: 4
end: 12
orientation: inline

Beta-helix
type: SequenceAnnotation
persistent identity: annotation2212458
display id: annotation2212458
version: 1
top level: BBa_K844008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K844008/annotation2212458/range2212458/1

https://synbiohub.org/public/igem/BBa_K844008/annotation2212458/range2212458/1
type: Range
persistent identity: range2212458
display id: range2212458
version: 1
direction: direction/forward
top level: BBa_K844008
start: 88
end: 97
orientation: inline

Elasticity Domain
type: SequenceAnnotation
persistent identity: annotation2212459
display id: annotation2212459
version: 1
top level: BBa_K844008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K844008/annotation2212459/range2212459/1

https://synbiohub.org/public/igem/BBa_K844008/annotation2212459/range2212459/1
type: Range
persistent identity: range2212459
display id: range2212459
version: 1
direction: direction/forward
top level: BBa_K844008
start: 88
end: 171
orientation: inline

Linker Domain
type: SequenceAnnotation
persistent identity: annotation2212464
display id: annotation2212464
version: 1
top level: BBa_K844008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K844008/annotation2212464/range2212464/1

https://synbiohub.org/public/igem/BBa_K844008/annotation2212464/range2212464/1
type: Range
persistent identity: range2212464
display id: range2212464
version: 1
direction: direction/forward
top level: BBa_K844008
start: 172
end: 188
orientation: inline

Elasticity Domain
type: SequenceAnnotation
persistent identity: annotation2212453
display id: annotation2212453
version: 1
top level: BBa_K844008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K844008/annotation2212453/range2212453/1

https://synbiohub.org/public/igem/BBa_K844008/annotation2212453/range2212453/1
type: Range
persistent identity: range2212453
display id: range2212453
version: 1
direction: direction/forward
top level: BBa_K844008
start: 4
end: 87
orientation: inline

Beta-spiral
type: SequenceAnnotation
persistent identity: annotation2212457
display id: annotation2212457
version: 1
top level: BBa_K844008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K844008/annotation2212457/range2212457/1

https://synbiohub.org/public/igem/BBa_K844008/annotation2212457/range2212457/1
type: Range
persistent identity: range2212457
display id: range2212457
version: 1
direction: direction/forward
top level: BBa_K844008
start: 72
end: 87
orientation: inline

BBa_I6105
type: ComponentDefinition
persistent identity: BBa I6105
display id: BBa_I6105
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter O_H Test R0077.E0430
creator: Randy Rettberg
created: 2004-04-21T11:00:00Z
modified: 2015-08-31T04:07:44Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_E0030
component: BBa_B0012
component: BBa_R0077
component: BBa_B0010
component: BBa_B0034
sequence annotation: BBa_E0030
sequence annotation: BBa_R0077
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_I6105_sequence/1

BBa_E0030
type: Component
persistent identity: component949860
display id: component949860
version: 1
top level: BBa_I6105
access: public
definition: https://synbiohub.org/public/igem/BBa_E0030/1

BBa_B0012
type: Component
persistent identity: component949875
display id: component949875
version: 1
top level: BBa_I6105
access: public
definition: BBa_B0012

BBa_R0077
type: Component
persistent identity: component949853
display id: component949853
version: 1
top level: BBa_I6105
access: public
definition: cinR

BBa_B0010
type: Component
persistent identity: component949865
display id: component949865
version: 1
top level: BBa_I6105
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component949858
display id: component949858
version: 1
top level: BBa_I6105
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation949860
display id: annotation949860
version: 1
top level: BBa_I6105
location: https://synbiohub.org/public/igem/BBa_I6105/annotation949860/range949860/1
component: BBa_E0030

https://synbiohub.org/public/igem/BBa_I6105/annotation949860/range949860/1
type: Range
persistent identity: range949860
display id: range949860
version: 1
direction: direction/forward
top level: BBa_I6105
start: 258
end: 980
orientation: inline

BBa_R0077
type: SequenceAnnotation
persistent identity: annotation949853
display id: annotation949853
version: 1
top level: BBa_I6105
location: https://synbiohub.org/public/igem/BBa_I6105/annotation949853/range949853/1
component: BBa_R0077

https://synbiohub.org/public/igem/BBa_I6105/annotation949853/range949853/1
type: Range
persistent identity: range949853
display id: range949853
version: 1
direction: direction/forward
top level: BBa_I6105
start: 1
end: 231
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation949875
display id: annotation949875
version: 1
top level: BBa_I6105
location: https://synbiohub.org/public/igem/BBa_I6105/annotation949875/range949875/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I6105/annotation949875/range949875/1
type: Range
persistent identity: range949875
display id: range949875
version: 1
direction: direction/forward
top level: BBa_I6105
start: 1077
end: 1117
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation949865
display id: annotation949865
version: 1
top level: BBa_I6105
location: https://synbiohub.org/public/igem/BBa_I6105/annotation949865/range949865/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I6105/annotation949865/range949865/1
type: Range
persistent identity: range949865
display id: range949865
version: 1
direction: direction/forward
top level: BBa_I6105
start: 989
end: 1068
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation949858
display id: annotation949858
version: 1
top level: BBa_I6105
location: https://synbiohub.org/public/igem/BBa_I6105/annotation949858/range949858/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I6105/annotation949858/range949858/1
type: Range
persistent identity: range949858
display id: range949858
version: 1
direction: direction/forward
top level: BBa_I6105
start: 240
end: 251
orientation: inline

BBa_I6070
type: ComponentDefinition
persistent identity: BBa I6070
display id: BBa_I6070
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter O_H Test R0053.E0432
creator: Caitlin Conboy and Jennifer Braff
created: 2004-03-17T12:00:00Z
modified: 2015-08-31T04:07:43Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_B0012
component: BBa_B0010
component: BBa_E0032
component: BBa_R0053
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_R0053
sequence annotation: BBa_E0032
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_I6070_sequence/1

BBa_B0012
type: Component
persistent identity: component949226
display id: component949226
version: 1
top level: BBa_I6070
access: public
definition: BBa_B0012

BBa_B0010
type: Component
persistent identity: component949216
display id: component949216
version: 1
top level: BBa_I6070
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_E0032
type: Component
persistent identity: component949208
display id: component949208
version: 1
top level: BBa_I6070
access: public
definition: YFP

BBa_R0053
type: Component
persistent identity: component949186
display id: component949186
version: 1
top level: BBa_I6070
access: public
definition: https://synbiohub.org/public/igem/BBa_R0053/1

BBa_B0034
type: Component
persistent identity: component949196
display id: component949196
version: 1
top level: BBa_I6070
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation949196
display id: annotation949196
version: 1
top level: BBa_I6070
location: https://synbiohub.org/public/igem/BBa_I6070/annotation949196/range949196/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I6070/annotation949196/range949196/1
type: Range
persistent identity: range949196
display id: range949196
version: 1
direction: direction/forward
top level: BBa_I6070
start: 63
end: 74
orientation: inline

BBa_R0053
type: SequenceAnnotation
persistent identity: annotation949186
display id: annotation949186
version: 1
top level: BBa_I6070
location: https://synbiohub.org/public/igem/BBa_I6070/annotation949186/range949186/1
component: BBa_R0053

https://synbiohub.org/public/igem/BBa_I6070/annotation949186/range949186/1
type: Range
persistent identity: range949186
display id: range949186
version: 1
direction: direction/forward
top level: BBa_I6070
start: 1
end: 54
orientation: inline

BBa_E0032
type: SequenceAnnotation
persistent identity: annotation949208
display id: annotation949208
version: 1
top level: BBa_I6070
location: https://synbiohub.org/public/igem/BBa_I6070/annotation949208/range949208/1
component: BBa_E0032

https://synbiohub.org/public/igem/BBa_I6070/annotation949208/range949208/1
type: Range
persistent identity: range949208
display id: range949208
version: 1
direction: direction/forward
top level: BBa_I6070
start: 81
end: 842
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation949216
display id: annotation949216
version: 1
top level: BBa_I6070
location: https://synbiohub.org/public/igem/BBa_I6070/annotation949216/range949216/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I6070/annotation949216/range949216/1
type: Range
persistent identity: range949216
display id: range949216
version: 1
direction: direction/forward
top level: BBa_I6070
start: 851
end: 930
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation949226
display id: annotation949226
version: 1
top level: BBa_I6070
location: https://synbiohub.org/public/igem/BBa_I6070/annotation949226/range949226/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I6070/annotation949226/range949226/1
type: Range
persistent identity: range949226
display id: range949226
version: 1
direction: direction/forward
top level: BBa_I6070
start: 939
end: 979
orientation: inline

BBa_K574005
type: ComponentDefinition
persistent identity: BBa K574005
display id: BBa_K574005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 3OC12HSL and YFP regulated by pBad
creator: Lei Wei
created: 2011-09-28T11:00:00Z
modified: 2015-05-08T01:12:44Z
discontinued: false
dominant: false
experience: experience/None
group u list: _745_
m user id: 0
owner id: 8529
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: pBAD + lasI + yfp.
mutable notes: Empty.
mutable provenance: Exsiting Parts.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_I13453
component: BBa_K081009
component: BBa_E0430
sequence annotation: BBa_K081009
sequence annotation: BBa_E0430
sequence annotation: BBa_I13453
sequence: https://synbiohub.org/public/igem/BBa_K574005_sequence/1

BBa_I13453
type: Component
persistent identity: component2268871
display id: component2268871
version: 1
top level: BBa_K574005
access: public
definition: BBa_I13453

BBa_K081009
type: Component
persistent identity: component2268879
display id: component2268879
version: 1
top level: BBa_K574005
access: public
definition: https://synbiohub.org/public/igem/BBa_K081009/1

BBa_E0430
type: Component
persistent identity: component2268889
display id: component2268889
version: 1
top level: BBa_K574005
access: public
definition: BBa_E0430

BBa_K081009
type: SequenceAnnotation
persistent identity: annotation2268879
display id: annotation2268879
version: 1
top level: BBa_K574005
location: https://synbiohub.org/public/igem/BBa_K574005/annotation2268879/range2268879/1
component: BBa_K081009

https://synbiohub.org/public/igem/BBa_K574005/annotation2268879/range2268879/1
type: Range
persistent identity: range2268879
display id: range2268879
version: 1
direction: direction/reverse
top level: BBa_K574005
start: 139
end: 826
orientation: inline

BBa_E0430
type: SequenceAnnotation
persistent identity: annotation2268889
display id: annotation2268889
version: 1
top level: BBa_K574005
location: https://synbiohub.org/public/igem/BBa_K574005/annotation2268889/range2268889/1
component: BBa_E0430

https://synbiohub.org/public/igem/BBa_K574005/annotation2268889/range2268889/1
type: Range
persistent identity: range2268889
display id: range2268889
version: 1
direction: direction/reverse
top level: BBa_K574005
start: 835
end: 1712
orientation: inline

BBa_I13453
type: SequenceAnnotation
persistent identity: annotation2268871
display id: annotation2268871
version: 1
top level: BBa_K574005
location: https://synbiohub.org/public/igem/BBa_K574005/annotation2268871/range2268871/1
component: BBa_I13453

https://synbiohub.org/public/igem/BBa_K574005/annotation2268871/range2268871/1
type: Range
persistent identity: range2268871
display id: range2268871
version: 1
direction: direction/reverse
top level: BBa_K574005
start: 1
end: 130
orientation: inline

BBa_K775003
type: ComponentDefinition
persistent identity: BBa K775003
display id: BBa_K775003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RI7-odr10 (diacetyl) chimeric GPCR with promoter and terminator
creator: Sietske Grijseels
created: 2012-09-04T11:00:00Z
modified: 2015-05-08T01:13:16Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1027_
m user id: 0
owner id: 12035
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: BBa_K775003 encodes a chimeric diacetyl-sensing G-protein coupled receptor which can be functionally expressed in S. cerevisiae. This chimeric receptor is composed of the ligand-binding domain of the C. elegans 7-transmembrane receptor ODR-10, flanked by the N- and C- terminals of the rat OR RI7.
mutable notes: -
mutable provenance: N-terminus: Olfactory receptor-like protein I7 (olr226), Rattus norvegicus (Rat) Ligand binding domain: C. elegans 7-transmembrane receptor ODR-10 U49449.1 C-terminus: Olfactory receptor-like protein I7 (olr226), Rattus norvegicus (Rat)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K775003_sequence/1

aiiA
type: ComponentDefinition
persistent identity: BBa C0060
display id: BBa_C0060
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: autoinducer inactivation enzyme from Bacillus; hydrolyzes acetyl homoserine lactone
creator: Vinay S Mahajan, Brian Chow, Peter Carr, Voichita D. Marinescu and Alexander D. Wissner-Gross
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Coding region for the autoinducer inactivation enzyme A (aiiA) LVA tagged. The gene was originally isolated from Bacillus sp. 240B1 and it encodes an enzyme that catalyzes the degradation of N-acyl homoserine lactones (AHLs)--quorum sensing autoinducers.
mutable notes: References (unparsed) here: Dong,Y.H., Xu,J.L., Li,X.Z. and Zhang,L.H.: AiiA, an enzyme that inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia carotovora, Proc. Natl. Acad. Sci. U.S.A. 97 (7), 3526-3531 (2000). http://www.pnas.org/cgi/content/full/97/7/3526 Lee SJ, Park SY, Lee JJ, Yum DY, Koo BT, Lee JK.: Genes encoding the N-acyl homoserine lactone-degrading enzyme are widespread in many subspecies of Bacillus thuringiensis, Appl Environ Microbiol 2002 Aug;68(8):3919-24. http://aem.asm.org/cgi/content/full/68/8/3919?view=full&pmid=12147491 References (unparsed) here: Dong,Y.H., Xu,J.L., Li,X.Z. and Zhang,L.H.: AiiA, an enzyme that inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia carotovora, Proc. Natl. Acad. Sci. U.S.A. 97 (7), 3526-3531 (2000). http://www.pnas.org/cgi/content/full/97/7/3526 Lee SJ, Park SY, Lee JJ, Yum DY, Koo BT, Lee JK.: Genes encoding the N-acyl homoserine lactone-degrading enzyme are widespread in many subspecies of Bacillus thuringiensis, Appl Environ Microbiol 2002 Aug;68(8):3919-24. http://aem.asm.org/cgi/content/full/68/8/3919?view=full&pmid=12147491 BBa_C0060 insert contains open reading frame (nucleotides 49-801) of the GeneBank sequence AF196486 followed by the LVA tag and two double stop codons inserted in the BioBrick prefix and suffix flanking regions. The original stop codon was TAG and in the present sequence it was substituted by TAATAA.
mutable provenance: AF196486 from Bacillus sp. 240B1 putative metallohydrolase (aiiA) gene. Dong,Y.H., Xu,J.L., Li,X.Z. and Zhang,L.H.: AiiA, an enzyme that inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia carotovora, Proc. Natl. Acad. Sci. U.S.A. 97 (7), 3526-3531 (2000).
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Help:Barcodes
sequence annotation: BBa_C0060
sequence annotation: LVA
sequence annotation: aiiA
sequence annotation: 2
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_C0060_sequence/1

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213987
display id: annotation2213987
version: 1
top level: aiiA
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_C0060/annotation2213987/range2213987/1

https://synbiohub.org/public/igem/BBa_C0060/annotation2213987/range2213987/1
type: Range
persistent identity: range2213987
display id: range2213987
version: 1
direction: direction/forward
top level: aiiA
start: 790
end: 814
orientation: inline

BBa_C0060
type: SequenceAnnotation
persistent identity: annotation7037
display id: annotation7037
version: 1
top level: aiiA
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_C0060/annotation7037/range7037/1

https://synbiohub.org/public/igem/BBa_C0060/annotation7037/range7037/1
type: Range
persistent identity: range7037
display id: range7037
version: 1
direction: direction/forward
top level: aiiA
start: 1
end: 789
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation1756
display id: annotation1756
version: 1
top level: aiiA
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_C0060/annotation1756/range1756/1

https://synbiohub.org/public/igem/BBa_C0060/annotation1756/range1756/1
type: Range
persistent identity: range1756
display id: range1756
version: 1
direction: direction/forward
top level: aiiA
start: 751
end: 783
orientation: inline

aiiA
type: SequenceAnnotation
persistent identity: annotation1757
display id: annotation1757
version: 1
top level: aiiA
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0060/annotation1757/range1757/1

https://synbiohub.org/public/igem/BBa_C0060/annotation1757/range1757/1
type: Range
persistent identity: range1757
display id: range1757
version: 1
direction: direction/forward
top level: aiiA
start: 1
end: 750
orientation: inline

2
type: SequenceAnnotation
persistent identity: annotation1755
display id: annotation1755
version: 1
top level: aiiA
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_C0060/annotation1755/range1755/1

https://synbiohub.org/public/igem/BBa_C0060/annotation1755/range1755/1
type: Range
persistent identity: range1755
display id: range1755
version: 1
direction: direction/forward
top level: aiiA
start: 784
end: 789
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1754
display id: annotation1754
version: 1
top level: aiiA
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_C0060/annotation1754/range1754/1

https://synbiohub.org/public/igem/BBa_C0060/annotation1754/range1754/1
type: Range
persistent identity: range1754
display id: range1754
version: 1
direction: direction/forward
top level: aiiA
start: 1
end: 3
orientation: inline

BBa_B0030
type: ComponentDefinition
persistent identity: BBa B0030
display id: BBa_B0030
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS.1 (strong) -- modified from R. Weiss
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _44_46_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info Brian Chow
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS-1\Strong
sequence annotation: BBa_B0030
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0030_sequence/1

RBS-1\Strong
type: SequenceAnnotation
persistent identity: annotation1701
display id: annotation1701
version: 1
top level: BBa_B0030
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1

https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
type: Range
persistent identity: range1701
display id: range1701
version: 1
direction: direction/forward
top level: BBa_B0030
start: 1
end: 15
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation7025
display id: annotation7025
version: 1
top level: BBa_B0030
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1

https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
type: Range
persistent identity: range7025
display id: range7025
version: 1
direction: direction/forward
top level: BBa_B0030
start: 1
end: 15
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1702
display id: annotation1702
version: 1
top level: BBa_B0030
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1

https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
type: Range
persistent identity: range1702
display id: range1702
version: 1
direction: direction/forward
top level: BBa_B0030
start: 8
end: 12
orientation: inline

p(tetR)
type: ComponentDefinition
persistent identity: BBa R0040
display id: BBa_R0040
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TetR repressible promoter
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Sequence for pTet inverting regulator driven by the TetR protein.
mutable notes: BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.
mutable provenance: Lutz, R., Bujard, H., Nucleic Acids Research (1997) 25, 1203-1210.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0040
sequence annotation: -35
sequence annotation: TetR 2
sequence annotation: -10
sequence annotation: TetR 1
sequence: https://synbiohub.org/public/igem/BBa_R0040_sequence/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1986784
display id: annotation1986784
version: 1
top level: p(tetR)
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1

https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
type: Range
persistent identity: range1986784
display id: range1986784
version: 1
direction: direction/forward
top level: p(tetR)
start: 1
end: 54
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1986785
display id: annotation1986785
version: 1
top level: p(tetR)
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1

https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
type: Range
persistent identity: range1986785
display id: range1986785
version: 1
direction: direction/forward
top level: p(tetR)
start: 20
end: 25
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation1986786
display id: annotation1986786
version: 1
top level: p(tetR)
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1

https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
type: Range
persistent identity: range1986786
display id: range1986786
version: 1
direction: direction/forward
top level: p(tetR)
start: 26
end: 44
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986787
display id: annotation1986787
version: 1
top level: p(tetR)
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1

https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
type: Range
persistent identity: range1986787
display id: range1986787
version: 1
direction: direction/forward
top level: p(tetR)
start: 43
end: 48
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1986783
display id: annotation1986783
version: 1
top level: p(tetR)
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1

https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
type: Range
persistent identity: range1986783
display id: range1986783
version: 1
direction: direction/forward
top level: p(tetR)
start: 1
end: 19
orientation: inline

BBa_K844006
type: ComponentDefinition
persistent identity: BBa K844006
display id: BBa_K844006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Spider Silk 1x Subunit "W" with Met (ATG) start codon
creator: Federico Carlos Rodriguez
created: 2012-10-01T11:00:00Z
modified: 2015-05-08T01:13:33Z
discontinued: false
dominant: false
group u list: _1104_
m user id: 0
owner id: 13908
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Spider silk subunit native sequence; contains two elasticity domains and one strength domain, contains added Met (ATG) on 5??? end.
mutable notes: none
mutable provenance: Created using mutagenesis PCR to add upstream Met (ATG) to part BBa_K844002
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Elasticity Domain
sequence annotation: Beta-spiral
sequence annotation: Beta-helix
sequence annotation: Start
sequence annotation: Linker Domain
sequence annotation: Strength Domain
sequence: https://synbiohub.org/public/igem/BBa_K844006_sequence/1

Elasticity Domain
type: SequenceAnnotation
persistent identity: annotation2212386
display id: annotation2212386
version: 1
top level: BBa_K844006
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K844006/annotation2212386/range2212386/1

https://synbiohub.org/public/igem/BBa_K844006/annotation2212386/range2212386/1
type: Range
persistent identity: range2212386
display id: range2212386
version: 1
direction: direction/forward
top level: BBa_K844006
start: 4
end: 87
orientation: inline

Beta-spiral
type: SequenceAnnotation
persistent identity: annotation2212394
display id: annotation2212394
version: 1
top level: BBa_K844006
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K844006/annotation2212394/range2212394/1

https://synbiohub.org/public/igem/BBa_K844006/annotation2212394/range2212394/1
type: Range
persistent identity: range2212394
display id: range2212394
version: 1
direction: direction/forward
top level: BBa_K844006
start: 98
end: 113
orientation: inline

Beta-spiral
type: SequenceAnnotation
persistent identity: annotation2212395
display id: annotation2212395
version: 1
top level: BBa_K844006
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K844006/annotation2212395/range2212395/1

https://synbiohub.org/public/igem/BBa_K844006/annotation2212395/range2212395/1
type: Range
persistent identity: range2212395
display id: range2212395
version: 1
direction: direction/forward
top level: BBa_K844006
start: 117
end: 132
orientation: inline

Beta-spiral
type: SequenceAnnotation
persistent identity: annotation2212388
display id: annotation2212388
version: 1
top level: BBa_K844006
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K844006/annotation2212388/range2212388/1

https://synbiohub.org/public/igem/BBa_K844006/annotation2212388/range2212388/1
type: Range
persistent identity: range2212388
display id: range2212388
version: 1
direction: direction/forward
top level: BBa_K844006
start: 13
end: 27
orientation: inline

Elasticity Domain
type: SequenceAnnotation
persistent identity: annotation2212393
display id: annotation2212393
version: 1
top level: BBa_K844006
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K844006/annotation2212393/range2212393/1

https://synbiohub.org/public/igem/BBa_K844006/annotation2212393/range2212393/1
type: Range
persistent identity: range2212393
display id: range2212393
version: 1
direction: direction/forward
top level: BBa_K844006
start: 88
end: 171
orientation: inline

Beta-spiral
type: SequenceAnnotation
persistent identity: annotation2212396
display id: annotation2212396
version: 1
top level: BBa_K844006
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K844006/annotation2212396/range2212396/1

https://synbiohub.org/public/igem/BBa_K844006/annotation2212396/range2212396/1
type: Range
persistent identity: range2212396
display id: range2212396
version: 1
direction: direction/forward
top level: BBa_K844006
start: 133
end: 147
orientation: inline

Beta-spiral
type: SequenceAnnotation
persistent identity: annotation2212397
display id: annotation2212397
version: 1
top level: BBa_K844006
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K844006/annotation2212397/range2212397/1

https://synbiohub.org/public/igem/BBa_K844006/annotation2212397/range2212397/1
type: Range
persistent identity: range2212397
display id: range2212397
version: 1
direction: direction/forward
top level: BBa_K844006
start: 156
end: 171
orientation: inline

Beta-helix
type: SequenceAnnotation
persistent identity: annotation2212387
display id: annotation2212387
version: 1
top level: BBa_K844006
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K844006/annotation2212387/range2212387/1

https://synbiohub.org/public/igem/BBa_K844006/annotation2212387/range2212387/1
type: Range
persistent identity: range2212387
display id: range2212387
version: 1
direction: direction/forward
top level: BBa_K844006
start: 4
end: 12
orientation: inline

Beta-spiral
type: SequenceAnnotation
persistent identity: annotation2212390
display id: annotation2212390
version: 1
top level: BBa_K844006
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K844006/annotation2212390/range2212390/1

https://synbiohub.org/public/igem/BBa_K844006/annotation2212390/range2212390/1
type: Range
persistent identity: range2212390
display id: range2212390
version: 1
direction: direction/forward
top level: BBa_K844006
start: 49
end: 63
orientation: inline

Beta-spiral
type: SequenceAnnotation
persistent identity: annotation2212391
display id: annotation2212391
version: 1
top level: BBa_K844006
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K844006/annotation2212391/range2212391/1

https://synbiohub.org/public/igem/BBa_K844006/annotation2212391/range2212391/1
type: Range
persistent identity: range2212391
display id: range2212391
version: 1
direction: direction/forward
top level: BBa_K844006
start: 72
end: 87
orientation: inline

Start
type: SequenceAnnotation
persistent identity: annotation2212385
display id: annotation2212385
version: 1
top level: BBa_K844006
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K844006/annotation2212385/range2212385/1

https://synbiohub.org/public/igem/BBa_K844006/annotation2212385/range2212385/1
type: Range
persistent identity: range2212385
display id: range2212385
version: 1
direction: direction/forward
top level: BBa_K844006
start: 1
end: 3
orientation: inline

Beta-spiral
type: SequenceAnnotation
persistent identity: annotation2212389
display id: annotation2212389
version: 1
top level: BBa_K844006
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K844006/annotation2212389/range2212389/1

https://synbiohub.org/public/igem/BBa_K844006/annotation2212389/range2212389/1
type: Range
persistent identity: range2212389
display id: range2212389
version: 1
direction: direction/forward
top level: BBa_K844006
start: 34
end: 48
orientation: inline

Beta-helix
type: SequenceAnnotation
persistent identity: annotation2212392
display id: annotation2212392
version: 1
top level: BBa_K844006
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K844006/annotation2212392/range2212392/1

https://synbiohub.org/public/igem/BBa_K844006/annotation2212392/range2212392/1
type: Range
persistent identity: range2212392
display id: range2212392
version: 1
direction: direction/forward
top level: BBa_K844006
start: 88
end: 97
orientation: inline

Linker Domain
type: SequenceAnnotation
persistent identity: annotation2212398
display id: annotation2212398
version: 1
top level: BBa_K844006
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K844006/annotation2212398/range2212398/1

https://synbiohub.org/public/igem/BBa_K844006/annotation2212398/range2212398/1
type: Range
persistent identity: range2212398
display id: range2212398
version: 1
direction: direction/forward
top level: BBa_K844006
start: 172
end: 188
orientation: inline

Strength Domain
type: SequenceAnnotation
persistent identity: annotation2212399
display id: annotation2212399
version: 1
top level: BBa_K844006
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K844006/annotation2212399/range2212399/1

https://synbiohub.org/public/igem/BBa_K844006/annotation2212399/range2212399/1
type: Range
persistent identity: range2212399
display id: range2212399
version: 1
direction: direction/forward
top level: BBa_K844006
start: 189
end: 207
orientation: inline

BBa_K876067
type: ComponentDefinition
persistent identity: BBa K876067
display id: BBa_K876067
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Term-Term-pLac-LuxR
creator: Zakaraya Ashour
created: 2012-10-01T11:00:00Z
modified: 2015-05-08T01:13:39Z
discontinued: false
dominant: true
group u list: _1138_
m user id: 0
owner id: 13835
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: TT-pLac-LuxR
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0015
component: BBa_C0062
component: BBa_R0010
sequence annotation: BBa_R0010
sequence annotation: BBa_B0015
sequence annotation: BBa_C0062
sequence: https://synbiohub.org/public/igem/BBa_K876067_sequence/1

BBa_B0015
type: Component
persistent identity: component2251300
display id: component2251300
version: 1
top level: BBa_K876067
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_C0062
type: Component
persistent identity: component2251310
display id: component2251310
version: 1
top level: BBa_K876067
access: public
definition: luxr

BBa_R0010
type: Component
persistent identity: component2251301
display id: component2251301
version: 1
top level: BBa_K876067
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2251301
display id: annotation2251301
version: 1
top level: BBa_K876067
location: https://synbiohub.org/public/igem/BBa_K876067/annotation2251301/range2251301/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K876067/annotation2251301/range2251301/1
type: Range
persistent identity: range2251301
display id: range2251301
version: 1
direction: direction/forward
top level: BBa_K876067
start: 138
end: 337
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2251300
display id: annotation2251300
version: 1
top level: BBa_K876067
location: https://synbiohub.org/public/igem/BBa_K876067/annotation2251300/range2251300/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K876067/annotation2251300/range2251300/1
type: Range
persistent identity: range2251300
display id: range2251300
version: 1
direction: direction/reverse
top level: BBa_K876067
start: 1
end: 129
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation2251310
display id: annotation2251310
version: 1
top level: BBa_K876067
location: https://synbiohub.org/public/igem/BBa_K876067/annotation2251310/range2251310/1
component: BBa_C0062

https://synbiohub.org/public/igem/BBa_K876067/annotation2251310/range2251310/1
type: Range
persistent identity: range2251310
display id: range2251310
version: 1
direction: direction/forward
top level: BBa_K876067
start: 344
end: 1099
orientation: inline

BBa_S0117
type: ComponentDefinition
persistent identity: BBa S0117
display id: BBa_S0117
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0031.C0053
creator: Caitlin Conboy and Jennifer Braff
created: 2003-12-04T12:00:00Z
modified: 2015-05-08T01:14:17Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_B0031
component: BBa_C0053
sequence annotation: BBa_B0031
sequence annotation: BBa_C0053
sequence: https://synbiohub.org/public/igem/BBa_S0117_sequence/1

BBa_B0031
type: Component
persistent identity: component939989
display id: component939989
version: 1
top level: BBa_S0117
access: public
definition: BBa_B0031

BBa_C0053
type: Component
persistent identity: component940001
display id: component940001
version: 1
top level: BBa_S0117
access: public
definition: c2 P22

BBa_B0031
type: SequenceAnnotation
persistent identity: annotation939989
display id: annotation939989
version: 1
top level: BBa_S0117
location: https://synbiohub.org/public/igem/BBa_S0117/annotation939989/range939989/1
component: BBa_B0031

https://synbiohub.org/public/igem/BBa_S0117/annotation939989/range939989/1
type: Range
persistent identity: range939989
display id: range939989
version: 1
direction: direction/forward
top level: BBa_S0117
start: 1
end: 14
orientation: inline

BBa_C0053
type: SequenceAnnotation
persistent identity: annotation940001
display id: annotation940001
version: 1
top level: BBa_S0117
location: https://synbiohub.org/public/igem/BBa_S0117/annotation940001/range940001/1
component: BBa_C0053

https://synbiohub.org/public/igem/BBa_S0117/annotation940001/range940001/1
type: Range
persistent identity: range940001
display id: range940001
version: 1
direction: direction/forward
top level: BBa_S0117
start: 21
end: 707
orientation: inline

BBa_K775002
type: ComponentDefinition
persistent identity: BBa K775002
display id: BBa_K775002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RI7-OR1G1 Isoamylacetate chimeric GPCR
creator: Sietske Grijseels
created: 2012-09-04T11:00:00Z
modified: 2015-05-08T01:13:16Z
discontinued: false
dominant: false
group u list: _1027_
m user id: 0
owner id: 12035
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: BBa_K775002 encodes a chimeric isoamylacetate-sensing G-protein coupled receptor which can be functionally expressed in S. cerevisiae. This chimeric receptor is composed of the ligand-binding domain of the mouse OR1G1, flanked by the N- and C- terminals of the rat OR RI7.
mutable notes: -
mutable provenance: N-terminus: Olfactory receptor-like protein I7 (olr226), Rattus norvegicus (Rat) Ligand binding domain: ORG1, (Homo sapiens) NM_003555 C-terminus: Olfactory receptor-like protein I7 (olr226), Rattus norvegicus (Rat)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K775002_sequence/1

BBa_K876000
type: ComponentDefinition
persistent identity: BBa K876000
display id: BBa_K876000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pLac-TetR
creator: Alec Spinhirne
created: 2012-09-29T11:00:00Z
modified: 2015-05-08T01:13:38Z
discontinued: false
dominant: false
group u list: _1138_
m user id: 0
owner id: 13834
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Constitutive producer for TetR, repressed by LacI
mutable notes: Biobrick placement
mutable provenance: Created from biobricks
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_R0010
component: BBa_C0040
sequence annotation: BBa_R0010
sequence annotation: BBa_C0040
sequence: https://synbiohub.org/public/igem/BBa_K876000_sequence/1

BBa_R0010
type: Component
persistent identity: component2245076
display id: component2245076
version: 1
top level: BBa_K876000
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_C0040
type: Component
persistent identity: component2245086
display id: component2245086
version: 1
top level: BBa_K876000
access: public
definition: tetR

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2245076
display id: annotation2245076
version: 1
top level: BBa_K876000
location: https://synbiohub.org/public/igem/BBa_K876000/annotation2245076/range2245076/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K876000/annotation2245076/range2245076/1
type: Range
persistent identity: range2245076
display id: range2245076
version: 1
direction: direction/forward
top level: BBa_K876000
start: 1
end: 200
orientation: inline

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation2245086
display id: annotation2245086
version: 1
top level: BBa_K876000
location: https://synbiohub.org/public/igem/BBa_K876000/annotation2245086/range2245086/1
component: BBa_C0040

https://synbiohub.org/public/igem/BBa_K876000/annotation2245086/range2245086/1
type: Range
persistent identity: range2245086
display id: range2245086
version: 1
direction: direction/reverse
top level: BBa_K876000
start: 207
end: 891
orientation: inline

BBa_I13002
type: ComponentDefinition
persistent identity: BBa I13002
display id: BBa_I13002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS B0031 w/ YFP (-LVA) TT
creator: Jay Blumling, Debra Lin, Madeleine Sheldon-dante, Fred Tan, MIT SMUG
created: 2004-06-27T11:00:00Z
modified: 2015-08-31T04:07:32Z
discontinued: false
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 101
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0012
component: BBa_B0031
component: BBa_E0030
component: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_E0030
sequence annotation: BBa_B0031
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_I13002_sequence/1

BBa_B0012
type: Component
persistent identity: component943469
display id: component943469
version: 1
top level: BBa_I13002
access: public
definition: BBa_B0012

BBa_B0031
type: Component
persistent identity: component943452
display id: component943452
version: 1
top level: BBa_I13002
access: public
definition: BBa_B0031

BBa_E0030
type: Component
persistent identity: component943454
display id: component943454
version: 1
top level: BBa_I13002
access: public
definition: https://synbiohub.org/public/igem/BBa_E0030/1

BBa_B0010
type: Component
persistent identity: component943459
display id: component943459
version: 1
top level: BBa_I13002
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation943469
display id: annotation943469
version: 1
top level: BBa_I13002
location: https://synbiohub.org/public/igem/BBa_I13002/annotation943469/range943469/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13002/annotation943469/range943469/1
type: Range
persistent identity: range943469
display id: range943469
version: 1
direction: direction/forward
top level: BBa_I13002
start: 840
end: 880
orientation: inline

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation943454
display id: annotation943454
version: 1
top level: BBa_I13002
location: https://synbiohub.org/public/igem/BBa_I13002/annotation943454/range943454/1
component: BBa_E0030

https://synbiohub.org/public/igem/BBa_I13002/annotation943454/range943454/1
type: Range
persistent identity: range943454
display id: range943454
version: 1
direction: direction/forward
top level: BBa_I13002
start: 21
end: 743
orientation: inline

BBa_B0031
type: SequenceAnnotation
persistent identity: annotation943452
display id: annotation943452
version: 1
top level: BBa_I13002
location: https://synbiohub.org/public/igem/BBa_I13002/annotation943452/range943452/1
component: BBa_B0031

https://synbiohub.org/public/igem/BBa_I13002/annotation943452/range943452/1
type: Range
persistent identity: range943452
display id: range943452
version: 1
direction: direction/forward
top level: BBa_I13002
start: 1
end: 14
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation943459
display id: annotation943459
version: 1
top level: BBa_I13002
location: https://synbiohub.org/public/igem/BBa_I13002/annotation943459/range943459/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13002/annotation943459/range943459/1
type: Range
persistent identity: range943459
display id: range943459
version: 1
direction: direction/forward
top level: BBa_I13002
start: 752
end: 831
orientation: inline

BBa_K737032
type: ComponentDefinition
persistent identity: BBa K737032
display id: BBa_K737032
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Spot42 based small RNA2
creator: Peiran Zhang and Minghao Gong
created: 2012-09-17T11:00:00Z
modified: 2015-05-08T01:13:07Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _986_
m user id: 0
owner id: 14291
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Spot42 is a multitarget small RNA that mediates the discoordinate expression of the E.coli galactose operon and other sugar metabolic pathway,such as galK,nanC,ytfJ,srlA,which are the 5??? leader sequence of the corresponding metabolic enzymes. Take galK for example,Spot42 causes translation repression by base pairing to RBS in the 5??? leader sequence,then block the recognition of the antiSD sequence on 30S subunit. It???s a very strong pairing(up to 20bases),but it only causes 2.6 fold repression according to Johannes H. Urban and Jo?? rg Vogel,for it doesn???t result in the degradation of the RNA complex by RNaseE(ssRNA degradation) and RNaseIII(dsRNA degradation),both of them are major enzymes that causes RNA degradation in vivo.Spot42-galK complex degrades in a slow and presently unclear way. The base pairing is initiated by the recognition of seed region,which plays a significant role in the repression efficiency.
mutable notes: No
mutable provenance: No
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence: https://synbiohub.org/public/igem/BBa_K737032_sequence/1

BBa_I13504
type: ComponentDefinition
persistent identity: BBa I13504
display id: BBa_I13504
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Screening plasmid intermediate
creator: jkm
created: 2005-05-30T11:00:00Z
modified: 2015-08-31T04:07:34Z
discontinued: false
dominant: true
experience: experience/None
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Built by Josh as an intermediate in screening plasmid construction.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_E0040
component: BBa_B0010
component: BBa_B0012
component: BBa_B0034
sequence annotation: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_B0012
sequence annotation: BBa_E0040
sequence: https://synbiohub.org/public/igem/BBa_I13504_sequence/1

BBa_E0040
type: Component
persistent identity: component1505069
display id: component1505069
version: 1
top level: BBa_I13504
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_B0010
type: Component
persistent identity: component1505074
display id: component1505074
version: 1
top level: BBa_I13504
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component1505084
display id: component1505084
version: 1
top level: BBa_I13504
access: public
definition: BBa_B0012

BBa_B0034
type: Component
persistent identity: component1505067
display id: component1505067
version: 1
top level: BBa_I13504
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1505074
display id: annotation1505074
version: 1
top level: BBa_I13504
location: https://synbiohub.org/public/igem/BBa_I13504/annotation1505074/range1505074/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13504/annotation1505074/range1505074/1
type: Range
persistent identity: range1505074
display id: range1505074
version: 1
direction: direction/forward
top level: BBa_I13504
start: 747
end: 826
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1505067
display id: annotation1505067
version: 1
top level: BBa_I13504
location: https://synbiohub.org/public/igem/BBa_I13504/annotation1505067/range1505067/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13504/annotation1505067/range1505067/1
type: Range
persistent identity: range1505067
display id: range1505067
version: 1
direction: direction/forward
top level: BBa_I13504
start: 1
end: 12
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1505084
display id: annotation1505084
version: 1
top level: BBa_I13504
location: https://synbiohub.org/public/igem/BBa_I13504/annotation1505084/range1505084/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13504/annotation1505084/range1505084/1
type: Range
persistent identity: range1505084
display id: range1505084
version: 1
direction: direction/forward
top level: BBa_I13504
start: 835
end: 875
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1505069
display id: annotation1505069
version: 1
top level: BBa_I13504
location: https://synbiohub.org/public/igem/BBa_I13504/annotation1505069/range1505069/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_I13504/annotation1505069/range1505069/1
type: Range
persistent identity: range1505069
display id: range1505069
version: 1
direction: direction/forward
top level: BBa_I13504
start: 19
end: 738
orientation: inline

PCaa3
type: ComponentDefinition
persistent identity: BBa K743002
display id: BBa_K743002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PCaa3 promoter and native rbs from Synechocystis PCC6803
creator: Juan Sim??n ??lamos
created: 2012-09-19T11:00:00Z
modified: 2015-05-08T01:13:09Z
discontinued: false
dominant: false
experience: experience/None
group u list: _994_
m user id: 0
owner id: 11584
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is the 100bp sequence upstream the Caa3 gene start codon, it contains putative rbs and promoter regions. Caa3 codes for a putative cytochrome aa3 controlling protein and its mRNA levels have shown to oscillate in a circadian manner, peaking at hour 9.
mutable notes: the desition of taking just 100bp upstream C
mutable provenance: this part was PCR amplified from Synechocystis chromosome, orf sll1898.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Pcaa3 promoter
sequence: https://synbiohub.org/public/igem/BBa_K743002_sequence/1

Pcaa3 promoter
type: SequenceAnnotation
persistent identity: annotation2188681
display id: annotation2188681
version: 1
top level: PCaa3
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K743002/annotation2188681/range2188681/1

https://synbiohub.org/public/igem/BBa_K743002/annotation2188681/range2188681/1
type: Range
persistent identity: range2188681
display id: range2188681
version: 1
direction: direction/forward
top level: PCaa3
start: 1
end: 101
orientation: inline

BBa_S03855
type: ComponentDefinition
persistent identity: BBa S03855
display id: BBa_S03855
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: I718017:E0840
creator: Eimad Shotar
created: 2007-10-25T11:00:00Z
modified: 2015-05-08T01:14:31Z
discontinued: false
dominant: true
group u list: _9_
m user id: 0
owner id: 1568
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_B0010
component: BBa_B0030
component: BBa_E0040
component: BBa_B0012
component: BBa_I718017
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_E0040
sequence annotation: BBa_I718017
sequence annotation: BBa_B0030
sequence: https://synbiohub.org/public/igem/BBa_S03855_sequence/1

BBa_B0010
type: Component
persistent identity: component1956429
display id: component1956429
version: 1
top level: BBa_S03855
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0030
type: Component
persistent identity: component1956425
display id: component1956425
version: 1
top level: BBa_S03855
access: public
definition: BBa_B0030

BBa_E0040
type: Component
persistent identity: component1956428
display id: component1956428
version: 1
top level: BBa_S03855
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_B0012
type: Component
persistent identity: component1956431
display id: component1956431
version: 1
top level: BBa_S03855
access: public
definition: BBa_B0012

BBa_I718017
type: Component
persistent identity: component1956423
display id: component1956423
version: 1
top level: BBa_S03855
access: public
definition: https://synbiohub.org/public/igem/BBa_I718017/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1956429
display id: annotation1956429
version: 1
top level: BBa_S03855
location: https://synbiohub.org/public/igem/BBa_S03855/annotation1956429/range1956429/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_S03855/annotation1956429/range1956429/1
type: Range
persistent identity: range1956429
display id: range1956429
version: 1
direction: direction/forward
top level: BBa_S03855
start: 792
end: 871
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1956431
display id: annotation1956431
version: 1
top level: BBa_S03855
location: https://synbiohub.org/public/igem/BBa_S03855/annotation1956431/range1956431/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_S03855/annotation1956431/range1956431/1
type: Range
persistent identity: range1956431
display id: range1956431
version: 1
direction: direction/forward
top level: BBa_S03855
start: 880
end: 920
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1956428
display id: annotation1956428
version: 1
top level: BBa_S03855
location: https://synbiohub.org/public/igem/BBa_S03855/annotation1956428/range1956428/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_S03855/annotation1956428/range1956428/1
type: Range
persistent identity: range1956428
display id: range1956428
version: 1
direction: direction/reverse
top level: BBa_S03855
start: 64
end: 783
orientation: inline

BBa_I718017
type: SequenceAnnotation
persistent identity: annotation1956423
display id: annotation1956423
version: 1
top level: BBa_S03855
location: https://synbiohub.org/public/igem/BBa_S03855/annotation1956423/range1956423/1
component: BBa_I718017

https://synbiohub.org/public/igem/BBa_S03855/annotation1956423/range1956423/1
type: Range
persistent identity: range1956423
display id: range1956423
version: 1
direction: direction/reverse
top level: BBa_S03855
start: 1
end: 34
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1956425
display id: annotation1956425
version: 1
top level: BBa_S03855
location: https://synbiohub.org/public/igem/BBa_S03855/annotation1956425/range1956425/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_S03855/annotation1956425/range1956425/1
type: Range
persistent identity: range1956425
display id: range1956425
version: 1
direction: direction/forward
top level: BBa_S03855
start: 43
end: 57
orientation: inline

BBa_I716208
type: ComponentDefinition
persistent identity: BBa I716208
display id: BBa_I716208
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [lox][terminator][lox]
creator: Samantha Liang
created: 2007-06-04T11:00:00Z
modified: 2015-08-31T04:07:51Z
discontinued: false
dominant: false
group u list: _110_
m user id: 0
owner id: 933
owning group id: 52
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: [lox][terminator][lox]
mutable notes: Digest pBca9145-I716206 (BamHI/AlwnI, 1694+553bp, Large) Digest pBca9145-Bca1114 (BglII/AlwnI, 1519+593bp, Small) Product is pBca9145-I716208.
mutable provenance: Digest pBca9145-I716206 (BamHI/AlwnI, 1694+553bp, Large) Digest pBca9145-Bca1114 (BglII/AlwnI, 1519+593bp, Small) Product is pBca9145-I716208.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence: https://synbiohub.org/public/igem/BBa_I716208_sequence/1

BBa_K779501
type: ComponentDefinition
persistent identity: BBa K779501
display id: BBa_K779501
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Long reporter top strand MammoBlock
creator: Kristjan Eerik Kaseniit
created: 2012-09-28T11:00:00Z
modified: 2015-05-08T01:13:18Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1033_
m user id: 0
owner id: 12684
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Reporter top strand. Modifications: 5' RQ quencher, 3' Alexa florophore, all bases modified with 2'O Methyl (ordered as a DNA oligo with Us instead of Ts). IDT DNA oligo order: /5IAbRQ/mCmA mCmCmC mAmCmU mCmCmU mCmUmC mCmCmA mCmCmA /3AlexF488N/
mutable notes: Sequence designed to be orthogonal to the HEK293 transcriptome, and to have a high melting temperature. See more details at the MIT iGEM wiki: http://2012.igem.org/Team:MIT
mutable provenance: De novo design.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence annotation: Domain Sa
sequence: https://synbiohub.org/public/igem/BBa_K779501_sequence/1

Domain Sa
type: SequenceAnnotation
persistent identity: annotation2204235
display id: annotation2204235
version: 1
top level: BBa_K779501
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K779501/annotation2204235/range2204235/1

https://synbiohub.org/public/igem/BBa_K779501/annotation2204235/range2204235/1
type: Range
persistent identity: range2204235
display id: range2204235
version: 1
direction: direction/forward
top level: BBa_K779501
start: 1
end: 20
orientation: inline

BBa_K909015
type: ComponentDefinition
persistent identity: BBa K909015
display id: BBa_K909015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: p-ABA overproduction composite without promoter
creator: Deborah Huber
created: 2012-09-21T11:00:00Z
modified: 2015-05-08T01:13:45Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1174_
m user id: 0
owner id: 14088
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: PABA is derived from the aromatic branch point precursor chorismate in two steps. Aminodeoxychorismate (ADC) synthase converts chorismate and glutamine to ADC and glutamate. The conversion of chorismate and glutamine to ADC and glutamate determines the rate and therefore overexpression of ADC synthase leads to overproduction of pABA. ADC synthase is composed of two subunits, PabA and PabB. Finally ADC lyase removes pyruvate from ADC, aromatizes the ring, and generates PABA.
mutable notes: pabB and pabA constitute an operon that can be controled by any promoter.
mutable provenance: S04039, K137055
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0010
component: BBa_B0012
component: BBa_K137005
component: BBa_B0034
component: BBa_K137006
sequence annotation: BBa_B0010
sequence annotation: BBa_K137005
sequence annotation: BBa_B0034
sequence annotation: BBa_B0012
sequence annotation: BBa_K137006
sequence: https://synbiohub.org/public/igem/BBa_K909015_sequence/1

BBa_B0010
type: Component
persistent identity: component2193611
display id: component2193611
version: 1
top level: BBa_K909015
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component2193613
display id: component2193613
version: 1
top level: BBa_K909015
access: public
definition: BBa_B0012

BBa_K137005
type: Component
persistent identity: component2193610
display id: component2193610
version: 1
top level: BBa_K909015
access: public
definition: https://synbiohub.org/public/igem/BBa_K137005/1

BBa_B0034
type: Component
persistent identity: component2193609
display id: component2193609
version: 1
top level: BBa_K909015
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K137006
type: Component
persistent identity: component2193607
display id: component2193607
version: 1
top level: BBa_K909015
access: public
definition: https://synbiohub.org/public/igem/BBa_K137006/1

BBa_B0034
type: Component
persistent identity: component2193606
display id: component2193606
version: 1
top level: BBa_K909015
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2193611
display id: annotation2193611
version: 1
top level: BBa_K909015
location: https://synbiohub.org/public/igem/BBa_K909015/annotation2193611/range2193611/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K909015/annotation2193611/range2193611/1
type: Range
persistent identity: range2193611
display id: range2193611
version: 1
direction: direction/forward
top level: BBa_K909015
start: 2528
end: 2607
orientation: inline

BBa_K137005
type: SequenceAnnotation
persistent identity: annotation2193610
display id: annotation2193610
version: 1
top level: BBa_K909015
location: https://synbiohub.org/public/igem/BBa_K909015/annotation2193610/range2193610/1
component: BBa_K137005

https://synbiohub.org/public/igem/BBa_K909015/annotation2193610/range2193610/1
type: Range
persistent identity: range2193610
display id: range2193610
version: 1
direction: direction/reverse
top level: BBa_K909015
start: 1935
end: 2519
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2193609
display id: annotation2193609
version: 1
top level: BBa_K909015
location: https://synbiohub.org/public/igem/BBa_K909015/annotation2193609/range2193609/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K909015/annotation2193609/range2193609/1
type: Range
persistent identity: range2193609
display id: range2193609
version: 1
direction: direction/reverse
top level: BBa_K909015
start: 1917
end: 1928
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2193613
display id: annotation2193613
version: 1
top level: BBa_K909015
location: https://synbiohub.org/public/igem/BBa_K909015/annotation2193613/range2193613/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K909015/annotation2193613/range2193613/1
type: Range
persistent identity: range2193613
display id: range2193613
version: 1
direction: direction/forward
top level: BBa_K909015
start: 2616
end: 2656
orientation: inline

BBa_K137006
type: SequenceAnnotation
persistent identity: annotation2193607
display id: annotation2193607
version: 1
top level: BBa_K909015
location: https://synbiohub.org/public/igem/BBa_K909015/annotation2193607/range2193607/1
component: BBa_K137006

https://synbiohub.org/public/igem/BBa_K909015/annotation2193607/range2193607/1
type: Range
persistent identity: range2193607
display id: range2193607
version: 1
direction: direction/reverse
top level: BBa_K909015
start: 19
end: 1908
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2193606
display id: annotation2193606
version: 1
top level: BBa_K909015
location: https://synbiohub.org/public/igem/BBa_K909015/annotation2193606/range2193606/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K909015/annotation2193606/range2193606/1
type: Range
persistent identity: range2193606
display id: range2193606
version: 1
direction: direction/reverse
top level: BBa_K909015
start: 1
end: 12
orientation: inline

BBa_K823016
type: ComponentDefinition
persistent identity: BBa K823016
display id: BBa_K823016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: lacZ full-length (RBS+ TERM-)
creator: Jara Radeck
created: 2012-09-10T11:00:00Z
modified: 2015-05-08T01:13:30Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1081_
m user id: 0
owner id: 11555
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Full-length lacZ with an RBS ahead. This is a copy of the Part:BBa_I732017. Only here the part is cloned in the standard vector pSB1C3 instead of the pSB1A2.
mutable notes: We changed the backbone vector of the part from pSB1A2 to pSB1C3
mutable provenance: Part:BBa_I732017
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_I732017
sequence annotation: BBa_I732017
sequence: https://synbiohub.org/public/igem/BBa_K823016_sequence/1

BBa_I732017
type: Component
persistent identity: component2182650
display id: component2182650
version: 1
top level: BBa_K823016
access: public
definition: https://synbiohub.org/public/igem/BBa_I732017/1

BBa_I732017
type: SequenceAnnotation
persistent identity: annotation2182650
display id: annotation2182650
version: 1
top level: BBa_K823016
location: https://synbiohub.org/public/igem/BBa_K823016/annotation2182650/range2182650/1
component: BBa_I732017

https://synbiohub.org/public/igem/BBa_K823016/annotation2182650/range2182650/1
type: Range
persistent identity: range2182650
display id: range2182650
version: 1
direction: direction/reverse
top level: BBa_K823016
start: 1
end: 3093
orientation: inline

BBa_I6042
type: ComponentDefinition
persistent identity: BBa I6042
display id: BBa_I6042
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter O_H Test R0062.E0430
creator: Caitlin Conboy and Jennifer Braff
created: 2004-03-16T12:00:00Z
modified: 2015-08-31T04:07:43Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_B0010
component: BBa_R0062
component: BBa_E0030
component: BBa_B0034
component: BBa_B0012
sequence annotation: BBa_E0030
sequence annotation: BBa_B0012
sequence annotation: BBa_R0062
sequence annotation: BBa_B0010
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_I6042_sequence/1

BBa_B0010
type: Component
persistent identity: component948549
display id: component948549
version: 1
top level: BBa_I6042
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_R0062
type: Component
persistent identity: component948534
display id: component948534
version: 1
top level: BBa_I6042
access: public
definition: lux pR

BBa_E0030
type: Component
persistent identity: component948544
display id: component948544
version: 1
top level: BBa_I6042
access: public
definition: https://synbiohub.org/public/igem/BBa_E0030/1

BBa_B0034
type: Component
persistent identity: component948542
display id: component948542
version: 1
top level: BBa_I6042
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: Component
persistent identity: component948559
display id: component948559
version: 1
top level: BBa_I6042
access: public
definition: BBa_B0012

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation948544
display id: annotation948544
version: 1
top level: BBa_I6042
location: https://synbiohub.org/public/igem/BBa_I6042/annotation948544/range948544/1
component: BBa_E0030

https://synbiohub.org/public/igem/BBa_I6042/annotation948544/range948544/1
type: Range
persistent identity: range948544
display id: range948544
version: 1
direction: direction/forward
top level: BBa_I6042
start: 82
end: 804
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation948559
display id: annotation948559
version: 1
top level: BBa_I6042
location: https://synbiohub.org/public/igem/BBa_I6042/annotation948559/range948559/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I6042/annotation948559/range948559/1
type: Range
persistent identity: range948559
display id: range948559
version: 1
direction: direction/forward
top level: BBa_I6042
start: 901
end: 941
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation948534
display id: annotation948534
version: 1
top level: BBa_I6042
location: https://synbiohub.org/public/igem/BBa_I6042/annotation948534/range948534/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_I6042/annotation948534/range948534/1
type: Range
persistent identity: range948534
display id: range948534
version: 1
direction: direction/forward
top level: BBa_I6042
start: 1
end: 55
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation948549
display id: annotation948549
version: 1
top level: BBa_I6042
location: https://synbiohub.org/public/igem/BBa_I6042/annotation948549/range948549/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I6042/annotation948549/range948549/1
type: Range
persistent identity: range948549
display id: range948549
version: 1
direction: direction/forward
top level: BBa_I6042
start: 813
end: 892
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation948542
display id: annotation948542
version: 1
top level: BBa_I6042
location: https://synbiohub.org/public/igem/BBa_I6042/annotation948542/range948542/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I6042/annotation948542/range948542/1
type: Range
persistent identity: range948542
display id: range948542
version: 1
direction: direction/forward
top level: BBa_I6042
start: 64
end: 75
orientation: inline

OmpR
type: ComponentDefinition
persistent identity: BBa R0084
display id: BBa_R0084
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter (OmpR, positive)
creator: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
created: 2004-01-27T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Positively regulated, OmpR-controlled promoter. This promoter is taken from the upstream region of ompF. Phosphorylated OmpR binds to the three operator sites and activates transcription.
mutable notes: The promoter includes three OmpR binding sites: F1, F2, and F3. The promoter starts at -107 and goes up to the transcriptional start site at +1. A distant fourth binding site, F4, has been deleted. It is involved in negative regulation of ompF (Head, Tardy and Kenney, 1998), so its deletion is intended to make the promoter solely activating. The efficacy of this promoter versus the ompC upstream region (BBa_R0082) or the truncated ompC upstream region (BBa_R0083) is currently unknown.
mutable provenance: NC_000913 E. coli K12
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: F2 OmpR
sequence annotation: F3 OmpR
sequence annotation: -10
sequence annotation: -35
sequence annotation: F1 OmpR
sequence: https://synbiohub.org/public/igem/BBa_R0084_sequence/1

F2 OmpR
type: SequenceAnnotation
persistent identity: annotation301309
display id: annotation301309
version: 1
top level: OmpR
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0084/annotation301309/range301309/1

https://synbiohub.org/public/igem/BBa_R0084/annotation301309/range301309/1
type: Range
persistent identity: range301309
display id: range301309
version: 1
direction: direction/forward
top level: OmpR
start: 31
end: 48
orientation: inline

F3 OmpR
type: SequenceAnnotation
persistent identity: annotation301313
display id: annotation301313
version: 1
top level: OmpR
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0084/annotation301313/range301313/1

https://synbiohub.org/public/igem/BBa_R0084/annotation301313/range301313/1
type: Range
persistent identity: range301313
display id: range301313
version: 1
direction: direction/forward
top level: OmpR
start: 51
end: 68
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation301318
display id: annotation301318
version: 1
top level: OmpR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0084/annotation301318/range301318/1

https://synbiohub.org/public/igem/BBa_R0084/annotation301318/range301318/1
type: Range
persistent identity: range301318
display id: range301318
version: 1
direction: direction/forward
top level: OmpR
start: 88
end: 93
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation301317
display id: annotation301317
version: 1
top level: OmpR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0084/annotation301317/range301317/1

https://synbiohub.org/public/igem/BBa_R0084/annotation301317/range301317/1
type: Range
persistent identity: range301317
display id: range301317
version: 1
direction: direction/forward
top level: OmpR
start: 73
end: 78
orientation: inline

F1 OmpR
type: SequenceAnnotation
persistent identity: annotation301308
display id: annotation301308
version: 1
top level: OmpR
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0084/annotation301308/range301308/1

https://synbiohub.org/public/igem/BBa_R0084/annotation301308/range301308/1
type: Range
persistent identity: range301308
display id: range301308
version: 1
direction: direction/forward
top level: OmpR
start: 11
end: 28
orientation: inline

BBa_K861042
type: ComponentDefinition
persistent identity: BBa K861042
display id: BBa_K861042
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: FadA, gene for fatty acid degradation from Salmonella enterica LT2
creator: Kuanwei Sheng
created: 2012-09-05T11:00:00Z
modified: 2015-05-08T01:13:35Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1121_
m user id: 0
owner id: 12357
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: FadA gene in S. enterica and in E.coli have similar function. Yet it is shown that S-FadA have far better efficiency compared to FadA in E.coli.(???S-??? is used to distinguish FadA gene in E.coli )
mutable notes: None
mutable provenance: E.coli str.K12 lab mutant DH5α
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K861042_sequence/1

BBa_I6101
type: ComponentDefinition
persistent identity: BBa I6101
display id: BBa_I6101
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter O_H Test R0071.E0430
creator: Randy Rettberg
created: 2004-04-21T11:00:00Z
modified: 2015-08-31T04:07:43Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_B0034
component: BBa_B0010
component: BBa_B0012
component: BBa_E0030
component: BBa_R0071
sequence annotation: BBa_B0034
sequence annotation: BBa_R0071
sequence annotation: BBa_B0012
sequence annotation: BBa_E0030
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_I6101_sequence/1

BBa_B0034
type: Component
persistent identity: component949718
display id: component949718
version: 1
top level: BBa_I6101
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component949725
display id: component949725
version: 1
top level: BBa_I6101
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component949735
display id: component949735
version: 1
top level: BBa_I6101
access: public
definition: BBa_B0012

BBa_E0030
type: Component
persistent identity: component949720
display id: component949720
version: 1
top level: BBa_I6101
access: public
definition: https://synbiohub.org/public/igem/BBa_E0030/1

BBa_R0071
type: Component
persistent identity: component949713
display id: component949713
version: 1
top level: BBa_I6101
access: public
definition: RhlR+C4

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation949718
display id: annotation949718
version: 1
top level: BBa_I6101
location: https://synbiohub.org/public/igem/BBa_I6101/annotation949718/range949718/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I6101/annotation949718/range949718/1
type: Range
persistent identity: range949718
display id: range949718
version: 1
direction: direction/forward
top level: BBa_I6101
start: 62
end: 73
orientation: inline

BBa_R0071
type: SequenceAnnotation
persistent identity: annotation949713
display id: annotation949713
version: 1
top level: BBa_I6101
location: https://synbiohub.org/public/igem/BBa_I6101/annotation949713/range949713/1
component: BBa_R0071

https://synbiohub.org/public/igem/BBa_I6101/annotation949713/range949713/1
type: Range
persistent identity: range949713
display id: range949713
version: 1
direction: direction/forward
top level: BBa_I6101
start: 1
end: 53
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation949735
display id: annotation949735
version: 1
top level: BBa_I6101
location: https://synbiohub.org/public/igem/BBa_I6101/annotation949735/range949735/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I6101/annotation949735/range949735/1
type: Range
persistent identity: range949735
display id: range949735
version: 1
direction: direction/forward
top level: BBa_I6101
start: 899
end: 939
orientation: inline

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation949720
display id: annotation949720
version: 1
top level: BBa_I6101
location: https://synbiohub.org/public/igem/BBa_I6101/annotation949720/range949720/1
component: BBa_E0030

https://synbiohub.org/public/igem/BBa_I6101/annotation949720/range949720/1
type: Range
persistent identity: range949720
display id: range949720
version: 1
direction: direction/forward
top level: BBa_I6101
start: 80
end: 802
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation949725
display id: annotation949725
version: 1
top level: BBa_I6101
location: https://synbiohub.org/public/igem/BBa_I6101/annotation949725/range949725/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I6101/annotation949725/range949725/1
type: Range
persistent identity: range949725
display id: range949725
version: 1
direction: direction/forward
top level: BBa_I6101
start: 811
end: 890
orientation: inline

BBa_S0107
type: ComponentDefinition
persistent identity: BBa S0107
display id: BBa_S0107
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0033.C0040
creator: Caitlin Conboy and Jennifer Braff
created: 2003-12-04T12:00:00Z
modified: 2015-05-08T01:14:17Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_B0033
component: BBa_C0040
sequence annotation: BBa_B0033
sequence annotation: BBa_C0040
sequence: https://synbiohub.org/public/igem/BBa_S0107_sequence/1

BBa_B0033
type: Component
persistent identity: component939815
display id: component939815
version: 1
top level: BBa_S0107
access: public
definition: BBa_B0033

BBa_C0040
type: Component
persistent identity: component939826
display id: component939826
version: 1
top level: BBa_S0107
access: public
definition: tetR

BBa_B0033
type: SequenceAnnotation
persistent identity: annotation939815
display id: annotation939815
version: 1
top level: BBa_S0107
location: https://synbiohub.org/public/igem/BBa_S0107/annotation939815/range939815/1
component: BBa_B0033

https://synbiohub.org/public/igem/BBa_S0107/annotation939815/range939815/1
type: Range
persistent identity: range939815
display id: range939815
version: 1
direction: direction/forward
top level: BBa_S0107
start: 1
end: 11
orientation: inline

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation939826
display id: annotation939826
version: 1
top level: BBa_S0107
location: https://synbiohub.org/public/igem/BBa_S0107/annotation939826/range939826/1
component: BBa_C0040

https://synbiohub.org/public/igem/BBa_S0107/annotation939826/range939826/1
type: Range
persistent identity: range939826
display id: range939826
version: 1
direction: direction/forward
top level: BBa_S0107
start: 18
end: 677
orientation: inline

BBa_I716210
type: ComponentDefinition
persistent identity: BBa I716210
display id: BBa_I716210
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: BamHI endonuclease without start codon
creator: Samantha Liang
created: 2007-06-04T11:00:00Z
modified: 2015-08-31T04:07:51Z
discontinued: false
dominant: false
experience: experience/None
group u list: _110_
m user id: 0
owner id: 933
owning group id: 52
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: BamHI endonuclease without start codon
mutable notes: PCR sil04F and sil04R on BamHI methylase/endonuclease region. (657 bp, BglII/XhoI) Sub into pBca9145-Bca1089 (BglII/XhoI,2063+696, L) Product is pBca9145-I716210 sil04F Forward BglII biobrick of BamHI ccaaaAGATCTgaagtagaaaaagagtttattactga sil04R Reverse XholI/BamHI biobrick of BamHI gctagCTCGAGttaGGATCCctatttgttttcaactttatc
mutable provenance: PCR sil04F and sil04R on BamHI methylase/endonuclease region. (657 bp, BglII/XhoI) Sub into pBca9145-Bca1089 (BglII/XhoI,2063+696, L) Product is pBca9145-I716210
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_I716210_sequence/1

BBa_I6050
type: ComponentDefinition
persistent identity: BBa I6050
display id: BBa_I6050
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter O_H Test R1051.E0430
creator: Caitlin Conboy and Jennifer Braff
created: 2004-03-16T12:00:00Z
modified: 2015-08-31T04:07:43Z
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_B0010
component: BBa_E0030
component: BBa_B0034
component: BBa_R1051
component: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_E0030
sequence annotation: BBa_R1051
sequence annotation: BBa_B0012
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_I6050_sequence/1

BBa_B0010
type: Component
persistent identity: component948699
display id: component948699
version: 1
top level: BBa_I6050
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_E0030
type: Component
persistent identity: component948694
display id: component948694
version: 1
top level: BBa_I6050
access: public
definition: https://synbiohub.org/public/igem/BBa_E0030/1

BBa_B0034
type: Component
persistent identity: component948692
display id: component948692
version: 1
top level: BBa_I6050
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R1051
type: Component
persistent identity: component948684
display id: component948684
version: 1
top level: BBa_I6050
access: public
definition: cI lam

BBa_B0012
type: Component
persistent identity: component948709
display id: component948709
version: 1
top level: BBa_I6050
access: public
definition: BBa_B0012

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation948699
display id: annotation948699
version: 1
top level: BBa_I6050
location: https://synbiohub.org/public/igem/BBa_I6050/annotation948699/range948699/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I6050/annotation948699/range948699/1
type: Range
persistent identity: range948699
display id: range948699
version: 1
direction: direction/forward
top level: BBa_I6050
start: 807
end: 886
orientation: inline

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation948694
display id: annotation948694
version: 1
top level: BBa_I6050
location: https://synbiohub.org/public/igem/BBa_I6050/annotation948694/range948694/1
component: BBa_E0030

https://synbiohub.org/public/igem/BBa_I6050/annotation948694/range948694/1
type: Range
persistent identity: range948694
display id: range948694
version: 1
direction: direction/forward
top level: BBa_I6050
start: 76
end: 798
orientation: inline

BBa_R1051
type: SequenceAnnotation
persistent identity: annotation948684
display id: annotation948684
version: 1
top level: BBa_I6050
location: https://synbiohub.org/public/igem/BBa_I6050/annotation948684/range948684/1
component: BBa_R1051

https://synbiohub.org/public/igem/BBa_I6050/annotation948684/range948684/1
type: Range
persistent identity: range948684
display id: range948684
version: 1
direction: direction/forward
top level: BBa_I6050
start: 1
end: 49
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation948709
display id: annotation948709
version: 1
top level: BBa_I6050
location: https://synbiohub.org/public/igem/BBa_I6050/annotation948709/range948709/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I6050/annotation948709/range948709/1
type: Range
persistent identity: range948709
display id: range948709
version: 1
direction: direction/forward
top level: BBa_I6050
start: 895
end: 935
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation948692
display id: annotation948692
version: 1
top level: BBa_I6050
location: https://synbiohub.org/public/igem/BBa_I6050/annotation948692/range948692/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I6050/annotation948692/range948692/1
type: Range
persistent identity: range948692
display id: range948692
version: 1
direction: direction/forward
top level: BBa_I6050
start: 58
end: 69
orientation: inline

BBa_I6080
type: ComponentDefinition
persistent identity: BBa I6080
display id: BBa_I6080
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter O_H Test R1051.E0432
creator: Caitlin Conboy and Jennifer Braff
created: 2004-03-17T12:00:00Z
modified: 2015-08-31T04:07:43Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_B0034
component: BBa_B0010
component: BBa_B0012
component: BBa_R1051
component: BBa_E0032
sequence annotation: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_R1051
sequence annotation: BBa_E0032
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_I6080_sequence/1

BBa_B0034
type: Component
persistent identity: component949446
display id: component949446
version: 1
top level: BBa_I6080
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component949466
display id: component949466
version: 1
top level: BBa_I6080
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component949476
display id: component949476
version: 1
top level: BBa_I6080
access: public
definition: BBa_B0012

BBa_R1051
type: Component
persistent identity: component949438
display id: component949438
version: 1
top level: BBa_I6080
access: public
definition: cI lam

BBa_E0032
type: Component
persistent identity: component949458
display id: component949458
version: 1
top level: BBa_I6080
access: public
definition: YFP

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation949466
display id: annotation949466
version: 1
top level: BBa_I6080
location: https://synbiohub.org/public/igem/BBa_I6080/annotation949466/range949466/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I6080/annotation949466/range949466/1
type: Range
persistent identity: range949466
display id: range949466
version: 1
direction: direction/forward
top level: BBa_I6080
start: 846
end: 925
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation949446
display id: annotation949446
version: 1
top level: BBa_I6080
location: https://synbiohub.org/public/igem/BBa_I6080/annotation949446/range949446/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I6080/annotation949446/range949446/1
type: Range
persistent identity: range949446
display id: range949446
version: 1
direction: direction/forward
top level: BBa_I6080
start: 58
end: 69
orientation: inline

BBa_R1051
type: SequenceAnnotation
persistent identity: annotation949438
display id: annotation949438
version: 1
top level: BBa_I6080
location: https://synbiohub.org/public/igem/BBa_I6080/annotation949438/range949438/1
component: BBa_R1051

https://synbiohub.org/public/igem/BBa_I6080/annotation949438/range949438/1
type: Range
persistent identity: range949438
display id: range949438
version: 1
direction: direction/forward
top level: BBa_I6080
start: 1
end: 49
orientation: inline

BBa_E0032
type: SequenceAnnotation
persistent identity: annotation949458
display id: annotation949458
version: 1
top level: BBa_I6080
location: https://synbiohub.org/public/igem/BBa_I6080/annotation949458/range949458/1
component: BBa_E0032

https://synbiohub.org/public/igem/BBa_I6080/annotation949458/range949458/1
type: Range
persistent identity: range949458
display id: range949458
version: 1
direction: direction/forward
top level: BBa_I6080
start: 76
end: 837
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation949476
display id: annotation949476
version: 1
top level: BBa_I6080
location: https://synbiohub.org/public/igem/BBa_I6080/annotation949476/range949476/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I6080/annotation949476/range949476/1
type: Range
persistent identity: range949476
display id: range949476
version: 1
direction: direction/forward
top level: BBa_I6080
start: 934
end: 974
orientation: inline

BBa_J61051
type: ComponentDefinition
persistent identity: BBa J61051
display id: BBa_J61051
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [Psal1]
creator: John Anderson
created: 2007-02-20T12:00:00Z
modified: 2015-08-31T02:03:00Z
discontinued: false
dominant: false
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Salicylate promoter and nahR
mutable notes: nonh
mutable provenance: non
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J61051_sequence/1

PT7-D
type: ComponentDefinition
persistent identity: BBa I716106
display id: BBa_I716106
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 Promoter (Mutant D)
creator: David Tulga
created: 2007-06-08T11:00:00Z
modified: 2015-08-31T04:07:51Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _110_
m user id: 0
owner id: 1694
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is the T7 Promoter which is activated when the T7 RNA Polymerase is produced. It provides very good PoPS (Polymerases Per Second) output, as well as it is highly orthogonal to E. Coli's natural transcription system. This one of the moderately active (Mutant D) variations we are using.
mutable notes: {{BerkiGEM2007-Biobrick2.0Intro}}
mutable provenance: This part was generated synthetically.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/T7
role: SO:0001207
sequence annotation: T7 Promoter D
sequence: https://synbiohub.org/public/igem/BBa_I716106_sequence/1

T7 Promoter D
type: SequenceAnnotation
persistent identity: annotation1954612
display id: annotation1954612
version: 1
top level: PT7-D
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I716106/annotation1954612/range1954612/1

https://synbiohub.org/public/igem/BBa_I716106/annotation1954612/range1954612/1
type: Range
persistent identity: range1954612
display id: range1954612
version: 1
direction: direction/forward
top level: PT7-D
start: 1
end: 29
orientation: inline

BBa_I732812
type: ComponentDefinition
persistent identity: BBa I732812
display id: BBa_I732812
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ARL2A0301 (RBS+, TERM-)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:59Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_I732112
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_I732112
sequence: https://synbiohub.org/public/igem/BBa_I732812_sequence/1

BBa_I732112
type: Component
persistent identity: component1946199
display id: component1946199
version: 1
top level: BBa_I732812
access: public
definition: https://synbiohub.org/public/igem/BBa_I732112/1

BBa_B0034
type: Component
persistent identity: component1946198
display id: component1946198
version: 1
top level: BBa_I732812
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1946198
display id: annotation1946198
version: 1
top level: BBa_I732812
location: https://synbiohub.org/public/igem/BBa_I732812/annotation1946198/range1946198/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I732812/annotation1946198/range1946198/1
type: Range
persistent identity: range1946198
display id: range1946198
version: 1
direction: direction/reverse
top level: BBa_I732812
start: 1
end: 12
orientation: inline

BBa_I732112
type: SequenceAnnotation
persistent identity: annotation1946199
display id: annotation1946199
version: 1
top level: BBa_I732812
location: https://synbiohub.org/public/igem/BBa_I732812/annotation1946199/range1946199/1
component: BBa_I732112

https://synbiohub.org/public/igem/BBa_I732812/annotation1946199/range1946199/1
type: Range
persistent identity: range1946199
display id: range1946199
version: 1
direction: direction/reverse
top level: BBa_I732812
start: 19
end: 1104
orientation: inline

ECFP
type: ComponentDefinition
persistent identity: BBa E0026
display id: BBa_E0026
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: enhanced cyan fluorescent protein derived from A. victoria GFP
creator: jcbraff
created: 2004-06-06T11:00:00Z
modified: 2015-08-31T04:07:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 61
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_E0026_sequence/1

BBa_K909009
type: ComponentDefinition
persistent identity: BBa K909009
display id: BBa_K909009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cDNA of UV-B sensing protein UVR8 from Arabidopsis thaliana
creator: Gintautas Vainorius
created: 2012-09-22T11:00:00Z
modified: 2015-05-08T01:13:45Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1174_
m user id: 0
owner id: 14094
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: UVR8 is responsible for UV-B detection in plants. In dark state UVR8 forms a dimer, which breaks into monomers upon exposure to UV-B light (280-315 nm). Later monomer induces transcriptional changes necessary for UV-B damage repairs and protection. In addition a BamHI site is inserted after ATG codon for fusions with other proteins (e.g. tetR-DNA binding domain), to use UVR8 as a UV-B sensing domain.
mutable notes: Contains two illegal PstI sites.
mutable provenance: cDNA of UVR8 from Arabidopsis thaliana was provided by Roman Ulm, University of Geneva.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: BamHI site
sequence: https://synbiohub.org/public/igem/BBa_K909009_sequence/1

BamHI site
type: SequenceAnnotation
persistent identity: annotation2202567
display id: annotation2202567
version: 1
top level: BBa_K909009
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K909009/annotation2202567/range2202567/1

https://synbiohub.org/public/igem/BBa_K909009/annotation2202567/range2202567/1
type: Range
persistent identity: range2202567
display id: range2202567
version: 1
direction: direction/forward
top level: BBa_K909009
start: 4
end: 9
orientation: inline

mCherry
type: ComponentDefinition
persistent identity: BBa J06504
display id: BBa_J06504
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: monomeric RFP optimized for bacteria
creator: ytwang
created: 2005-07-17T11:00:00Z
modified: 2016-01-25T01:05:28Z
discontinued: false
dominant: false
experience: experience/None
group u list: _20_
m user id: 4206
owner id: 340
owning group id: 20
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: mRFP (DsRed) derived, altered to be a biobrick by removing a PstI site and adding in the ends.
mutable notes: Made a point mutation to eliminate a PstI site in the middle and then added BioBrick ends using PCR. Sequenced using primers that bind to the pSB1A2 plasmid on either side of the brick.
mutable provenance: mCherry from Roger Y. Tsien's lab, altered to be BioBrick compatible
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: mCherry
sequence annotation: C->T (removing PstI site)
sequence: https://synbiohub.org/public/igem/BBa_J06504_sequence/1

mCherry
type: SequenceAnnotation
persistent identity: annotation1585829
display id: annotation1585829
version: 1
top level: mCherry
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J06504/annotation1585829/range1585829/1

https://synbiohub.org/public/igem/BBa_J06504/annotation1585829/range1585829/1
type: Range
persistent identity: range1585829
display id: range1585829
version: 1
direction: direction/forward
top level: mCherry
start: 1
end: 711
orientation: inline

C->T (removing PstI site)
type: SequenceAnnotation
persistent identity: annotation1585833
display id: annotation1585833
version: 1
top level: mCherry
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_J06504/annotation1585833/range1585833/1

https://synbiohub.org/public/igem/BBa_J06504/annotation1585833/range1585833/1
type: Range
persistent identity: range1585833
display id: range1585833
version: 1
direction: direction/forward
top level: mCherry
start: 352
end: 352
orientation: inline

Cel5A
type: ComponentDefinition
persistent identity: BBa K805011
display id: BBa_K805011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cel5A, endoglucanases
creator: Liu Ping
created: 2012-09-19T11:00:00Z
modified: 2015-06-19T01:48:30Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1062_
m user id: 4206
owner id: 11895
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: test
mutable notes: test
mutable provenance: yes
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: protein
sequence annotation: start codon
sequence: https://synbiohub.org/public/igem/BBa_K805011_sequence/1

protein
type: SequenceAnnotation
persistent identity: annotation2200608
display id: annotation2200608
version: 1
top level: Cel5A
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K805011/annotation2200608/range2200608/1

https://synbiohub.org/public/igem/BBa_K805011/annotation2200608/range2200608/1
type: Range
persistent identity: range2200608
display id: range2200608
version: 1
direction: direction/forward
top level: Cel5A
start: 4
end: 1233
orientation: inline

start codon
type: SequenceAnnotation
persistent identity: annotation2200607
display id: annotation2200607
version: 1
top level: Cel5A
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K805011/annotation2200607/range2200607/1

https://synbiohub.org/public/igem/BBa_K805011/annotation2200607/range2200607/1
type: Range
persistent identity: range2200607
display id: range2200607
version: 1
direction: direction/forward
top level: Cel5A
start: 1
end: 3
orientation: inline

BBa_K863021
type: ComponentDefinition
persistent identity: BBa K863021
display id: BBa_K863021
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: bhal laccase from Bacillus halodurans
creator: Isabel Huber
created: 2012-09-22T11:00:00Z
modified: 2015-05-08T01:13:36Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1123_
m user id: 0
owner id: 12844
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: bhal laccase from Bacillus halodurans
mutable notes: test
mutable provenance: Bacillus halodurans C-125
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: CDS
sequence annotation: TAA
sequence annotation: ATG
sequence: https://synbiohub.org/public/igem/BBa_K863021_sequence/1

CDS
type: SequenceAnnotation
persistent identity: annotation2213229
display id: annotation2213229
version: 1
top level: BBa_K863021
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K863021/annotation2213229/range2213229/1

https://synbiohub.org/public/igem/BBa_K863021/annotation2213229/range2213229/1
type: Range
persistent identity: range2213229
display id: range2213229
version: 1
direction: direction/forward
top level: BBa_K863021
start: 1
end: 1503
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2213231
display id: annotation2213231
version: 1
top level: BBa_K863021
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K863021/annotation2213231/range2213231/1

https://synbiohub.org/public/igem/BBa_K863021/annotation2213231/range2213231/1
type: Range
persistent identity: range2213231
display id: range2213231
version: 1
direction: direction/forward
top level: BBa_K863021
start: 1501
end: 1503
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2213230
display id: annotation2213230
version: 1
top level: BBa_K863021
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K863021/annotation2213230/range2213230/1

https://synbiohub.org/public/igem/BBa_K863021/annotation2213230/range2213230/1
type: Range
persistent identity: range2213230
display id: range2213230
version: 1
direction: direction/forward
top level: BBa_K863021
start: 1
end: 3
orientation: inline

BBa_I13453
type: ComponentDefinition
persistent identity: BBa I13453
display id: BBa_I13453
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pbad promoter
creator: jkm
created: 2005-05-24T11:00:00Z
modified: 2015-08-31T04:07:34Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: PBad promoter from I0500 without AraC.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I13453_sequence/1

BBa_M30011
type: ComponentDefinition
persistent identity: BBa M30011
display id: BBa_M30011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: OmpR controlled mRFP
creator: natalie kuldell
created: 2005-12-14T12:00:00Z
modified: 2015-09-17T06:41:23Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _45_1_
m user id: 25546
owner id: 314
owning group id: 1
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Positively regulated, OmpR-controlled promoter from OmpC gene (R0082) directing transcription of mRFP (I13507). Constructed with standard assembly method except R0082 was isolated using VF and VR-directed PCR. Consequently, there may be silent mutations that were inadvertently introduced. An alternative clone is M30012. Cells bearing this construct are pale pink.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_B0010
component: BBa_R0082
component: BBa_B0034
component: BBa_B0012
component: BBa_E1010
sequence annotation: BBa_B0012
sequence annotation: BBa_E1010
sequence annotation: BBa_R0082
sequence annotation: BBa_B0010
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_M30011_sequence/1

BBa_B0010
type: Component
persistent identity: component1768986
display id: component1768986
version: 1
top level: BBa_M30011
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_R0082
type: Component
persistent identity: component1768968
display id: component1768968
version: 1
top level: BBa_M30011
access: public
definition: OmpR

BBa_B0034
type: Component
persistent identity: component1768973
display id: component1768973
version: 1
top level: BBa_M30011
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: Component
persistent identity: component1768996
display id: component1768996
version: 1
top level: BBa_M30011
access: public
definition: BBa_B0012

BBa_E1010
type: Component
persistent identity: component1768980
display id: component1768980
version: 1
top level: BBa_M30011
access: public
definition: https://synbiohub.org/public/igem/BBa_E1010/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1768996
display id: annotation1768996
version: 1
top level: BBa_M30011
location: https://synbiohub.org/public/igem/BBa_M30011/annotation1768996/range1768996/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_M30011/annotation1768996/range1768996/1
type: Range
persistent identity: range1768996
display id: range1768996
version: 1
direction: direction/forward
top level: BBa_M30011
start: 937
end: 977
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation1768980
display id: annotation1768980
version: 1
top level: BBa_M30011
location: https://synbiohub.org/public/igem/BBa_M30011/annotation1768980/range1768980/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_M30011/annotation1768980/range1768980/1
type: Range
persistent identity: range1768980
display id: range1768980
version: 1
direction: direction/forward
top level: BBa_M30011
start: 135
end: 815
orientation: inline

BBa_R0082
type: SequenceAnnotation
persistent identity: annotation1768968
display id: annotation1768968
version: 1
top level: BBa_M30011
location: https://synbiohub.org/public/igem/BBa_M30011/annotation1768968/range1768968/1
component: BBa_R0082

https://synbiohub.org/public/igem/BBa_M30011/annotation1768968/range1768968/1
type: Range
persistent identity: range1768968
display id: range1768968
version: 1
direction: direction/forward
top level: BBa_M30011
start: 1
end: 108
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1768986
display id: annotation1768986
version: 1
top level: BBa_M30011
location: https://synbiohub.org/public/igem/BBa_M30011/annotation1768986/range1768986/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_M30011/annotation1768986/range1768986/1
type: Range
persistent identity: range1768986
display id: range1768986
version: 1
direction: direction/forward
top level: BBa_M30011
start: 849
end: 928
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1768973
display id: annotation1768973
version: 1
top level: BBa_M30011
location: https://synbiohub.org/public/igem/BBa_M30011/annotation1768973/range1768973/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_M30011/annotation1768973/range1768973/1
type: Range
persistent identity: range1768973
display id: range1768973
version: 1
direction: direction/forward
top level: BBa_M30011
start: 117
end: 128
orientation: inline

lacI+pL
type: ComponentDefinition
persistent identity: BBa R0011
display id: BBa_R0011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter (lacI regulated, lambda pL hybrid)
creator: Neelaksh Varshney, Grace Kenney, Daniel Shen, Samantha Sutton
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong promotion that can nevertheless be tightly repressed by LacI, the Lac inhibitor (i.e. repressor) (<bb_part>BBa_C0010</bb_part>) ([LUTZ97]). The activity of the promoter can be regulated over a >600-fold range by IPTG in E.Coli DH5-alpha-Z1 (same paper reference).
mutable notes: hybrid promoter design to create strong promoter that is, at the same time, highly repressible. note that the upstream operator installed in this hybrid is slightly different than the one in the original source (Lutz and Bujard, 1997). the most upstream operator region is slightly truncated in the represillator version, so that both operators in the hybrid are the same sequence. see references for details. also, the sequence has been truncated after the transcriptional start site. LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and increase transcription. This part is incompatible with environments containing lactose or lactose analogs.
mutable provenance: represillator of Elowitz and Leibler (2000)
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0011
sequence annotation: lac O1
sequence annotation: -10
sequence annotation: -35
sequence: https://synbiohub.org/public/igem/BBa_R0011_sequence/1

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation7064
display id: annotation7064
version: 1
top level: lacI+pL
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_R0011/annotation7064/range7064/1

https://synbiohub.org/public/igem/BBa_R0011/annotation7064/range7064/1
type: Range
persistent identity: range7064
display id: range7064
version: 1
direction: direction/forward
top level: lacI+pL
start: 1
end: 54
orientation: inline

lac O1
type: SequenceAnnotation
persistent identity: annotation2001
display id: annotation2001
version: 1
top level: lacI+pL
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2001/range2001/1

https://synbiohub.org/public/igem/BBa_R0011/annotation2001/range2001/1
type: Range
persistent identity: range2001
display id: range2001
version: 1
direction: direction/forward
top level: lacI+pL
start: 26
end: 42
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2002
display id: annotation2002
version: 1
top level: lacI+pL
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2002/range2002/1

https://synbiohub.org/public/igem/BBa_R0011/annotation2002/range2002/1
type: Range
persistent identity: range2002
display id: range2002
version: 1
direction: direction/forward
top level: lacI+pL
start: 43
end: 48
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2000
display id: annotation2000
version: 1
top level: lacI+pL
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2000/range2000/1

https://synbiohub.org/public/igem/BBa_R0011/annotation2000/range2000/1
type: Range
persistent identity: range2000
display id: range2000
version: 1
direction: direction/forward
top level: lacI+pL
start: 20
end: 25
orientation: inline

lac O1
type: SequenceAnnotation
persistent identity: annotation1999
display id: annotation1999
version: 1
top level: lacI+pL
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_R0011/annotation1999/range1999/1

https://synbiohub.org/public/igem/BBa_R0011/annotation1999/range1999/1
type: Range
persistent identity: range1999
display id: range1999
version: 1
direction: direction/forward
top level: lacI+pL
start: 3
end: 19
orientation: inline

BBa_K786003
type: ComponentDefinition
persistent identity: BBa K786003
display id: BBa_K786003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Sensory rhodopsin I (SRI) with HtrII & Tar, sensitive to orange light
creator: Leung Wai Tak
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:22Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1041_
m user id: 0
owner id: 14285
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Sensory rhodopsin I fusion with HtrII & Tar, which is sensitive to orange light, with sensing spectra covering from 590-650 nm. We used it for sensing blur-green light to achieve phototaxis and switch for downstream gene expression.
mutable notes: Pfam (version 26.0) was used to predict the domain of fusion protein. Linker was refereed and modified from previous research study of SRI fusion with HtrII.
mutable provenance: From genomic sequence of bacterial N. pharaonis (DSM 2160) and E.coli K12.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: B0015
sequence annotation: SRI-HtrI-Tar
sequence annotation: RBS 1.0
sequence annotation: Constitutive promoter BBa_J23100
sequence: https://synbiohub.org/public/igem/BBa_K786003_sequence/1

B0015
type: SequenceAnnotation
persistent identity: annotation2199719
display id: annotation2199719
version: 1
top level: BBa_K786003
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K786003/annotation2199719/range2199719/1

https://synbiohub.org/public/igem/BBa_K786003/annotation2199719/range2199719/1
type: Range
persistent identity: range2199719
display id: range2199719
version: 1
direction: direction/forward
top level: BBa_K786003
start: 2029
end: 2158
orientation: inline

SRI-HtrI-Tar
type: SequenceAnnotation
persistent identity: annotation2199706
display id: annotation2199706
version: 1
top level: BBa_K786003
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K786003/annotation2199706/range2199706/1

https://synbiohub.org/public/igem/BBa_K786003/annotation2199706/range2199706/1
type: Range
persistent identity: range2199706
display id: range2199706
version: 1
direction: direction/forward
top level: BBa_K786003
start: 68
end: 1992
orientation: inline

RBS 1.0
type: SequenceAnnotation
persistent identity: annotation2190186
display id: annotation2190186
version: 1
top level: BBa_K786003
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K786003/annotation2190186/range2190186/1

https://synbiohub.org/public/igem/BBa_K786003/annotation2190186/range2190186/1
type: Range
persistent identity: range2190186
display id: range2190186
version: 1
direction: direction/forward
top level: BBa_K786003
start: 42
end: 61
orientation: inline

Constitutive promoter BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2190185
display id: annotation2190185
version: 1
top level: BBa_K786003
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K786003/annotation2190185/range2190185/1

https://synbiohub.org/public/igem/BBa_K786003/annotation2190185/range2190185/1
type: Range
persistent identity: range2190185
display id: range2190185
version: 1
direction: direction/forward
top level: BBa_K786003
start: 1
end: 41
orientation: inline

BBa_K737031
type: ComponentDefinition
persistent identity: BBa K737031
display id: BBa_K737031
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Spot42 based small RNA1
creator: Peiran Zhang and Minghao Gong
created: 2012-09-17T11:00:00Z
modified: 2015-05-08T01:13:07Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _986_
m user id: 0
owner id: 14291
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Spot42 is a multitarget small RNA that mediates the discoordinate expression of the E.coli galactose operon and other sugar metabolic pathway,such as galK,nanC,ytfJ,srlA,which are the 5??? leader sequence of the corresponding metabolic enzymes. Take galK for example,Spot42 causes translation repression by base pairing to RBS in the 5??? leader sequence,then block the recognition of the antiSD sequence on 30S subunit. It???s a very strong pairing(up to 20bases),but it only causes 2.6 fold repression according to Johannes H. Urban and Jo?? rg Vogel,for it doesn???t result in the degradation of the RNA complex by RNaseE(ssRNA degradation) and RNaseIII(dsRNA degradation),both of them are major enzymes that causes RNA degradation in vivo.Spot42-galK complex degrades in a slow and presently unclear way. The base pairing is initiated by the recognition of seed region,which plays a significant role in the repression efficiency.
mutable notes: No
mutable provenance: No
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence: https://synbiohub.org/public/igem/BBa_K737031_sequence/1

LacIM
type: ComponentDefinition
persistent identity: BBa K819012
display id: BBa_K819012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Luminesensor repressible LacIM generator
creator: ZHANG Hong
created: 2012-09-02T11:00:00Z
modified: 2015-05-08T01:13:29Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1077_
m user id: 0
owner id: 13785
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: LacI mutant driven by luminesensor repressible SulA promoter in 408 form.
mutable notes: B0034, CDS of LacI mutant and B0015 were connected directly to SulA promoter in 408 form.
mutable provenance: Sequene of the LacI mutant is from Partregistry.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: SulA408 Promoter
sequence annotation: LacIM(K395400)
sequence: https://synbiohub.org/public/igem/BBa_K819012_sequence/1

SulA408 Promoter
type: SequenceAnnotation
persistent identity: annotation2181628
display id: annotation2181628
version: 1
top level: LacIM
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K819012/annotation2181628/range2181628/1

https://synbiohub.org/public/igem/BBa_K819012/annotation2181628/range2181628/1
type: Range
persistent identity: range2181628
display id: range2181628
version: 1
direction: direction/forward
top level: LacIM
start: 1
end: 64
orientation: inline

LacIM(K395400)
type: SequenceAnnotation
persistent identity: annotation2181627
display id: annotation2181627
version: 1
top level: LacIM
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K819012/annotation2181627/range2181627/1

https://synbiohub.org/public/igem/BBa_K819012/annotation2181627/range2181627/1
type: Range
persistent identity: range2181627
display id: range2181627
version: 1
direction: direction/forward
top level: LacIM
start: 65
end: 1292
orientation: inline

BBa_K864680
type: ComponentDefinition
persistent identity: BBa K864680
display id: BBa_K864680
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: tetM
creator: Erik Lundin
created: 2012-09-25T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: tetM
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: tetM
sequence: https://synbiohub.org/public/igem/BBa_K864680_sequence/1

tetM
type: SequenceAnnotation
persistent identity: annotation2214190
display id: annotation2214190
version: 1
top level: BBa_K864680
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K864680/annotation2214190/range2214190/1

https://synbiohub.org/public/igem/BBa_K864680/annotation2214190/range2214190/1
type: Range
persistent identity: range2214190
display id: range2214190
version: 1
direction: direction/forward
top level: BBa_K864680
start: 1
end: 1920
orientation: inline

BBa_K822002
type: ComponentDefinition
persistent identity: BBa K822002
display id: BBa_K822002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Colicin E1 + immunoprotein
creator: Jarle Pahr
created: 2012-09-22T11:00:00Z
modified: 2015-05-08T01:13:29Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1080_
m user id: 0
owner id: 11627
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Colicin E1 operon of E. coli expressed under a constitutive promoter (BBa_J23119).
mutable notes: BBa_K150007 was not available from the registry, so its sequence was cloned from K150009 by PCR.
mutable provenance: BBa_J23119, BBa_B0034 and K150009/BBa_K150007.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: ColE1 protein
sequence annotation: ColE1 immunity protein
sequence annotation: Kil protein fragment
sequence: https://synbiohub.org/public/igem/BBa_K822002_sequence/1

ColE1 protein
type: SequenceAnnotation
persistent identity: annotation2197333
display id: annotation2197333
version: 1
top level: BBa_K822002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K822002/annotation2197333/range2197333/1

https://synbiohub.org/public/igem/BBa_K822002/annotation2197333/range2197333/1
type: Range
persistent identity: range2197333
display id: range2197333
version: 1
direction: direction/forward
top level: BBa_K822002
start: 1
end: 1569
orientation: inline

ColE1 immunity protein
type: SequenceAnnotation
persistent identity: annotation2197334
display id: annotation2197334
version: 1
top level: BBa_K822002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K822002/annotation2197334/range2197334/1

https://synbiohub.org/public/igem/BBa_K822002/annotation2197334/range2197334/1
type: Range
persistent identity: range2197334
display id: range2197334
version: 1
direction: direction/reverse
top level: BBa_K822002
start: 1566
end: 1907
orientation: inline

Kil protein fragment
type: SequenceAnnotation
persistent identity: annotation2197335
display id: annotation2197335
version: 1
top level: BBa_K822002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K822002/annotation2197335/range2197335/1

https://synbiohub.org/public/igem/BBa_K822002/annotation2197335/range2197335/1
type: Range
persistent identity: range2197335
display id: range2197335
version: 1
direction: direction/forward
top level: BBa_K822002
start: 1955
end: 2050
orientation: inline

BBa_K777117
type: ComponentDefinition
persistent identity: BBa K777117
display id: BBa_K777117
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: motB
creator: Team Goettingen
created: 2012-09-17T11:00:00Z
modified: 2015-05-08T01:13:16Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1029_
m user id: 0
owner id: 12106
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This gene codes for MotB, a part of the flagellar motor. MotA and MotB constitute the stator and are required for the flow of ions and torque generation.
mutable notes: * motA was amplified from genomic DNA of E. coli DH10B via PCR using the following primers: ** Fwd: GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAAGAATCAAGCGCATCC ** Rev: GTTTCTTCCTGCAGCGGCCGCTACTAGTATCACCTCGGTTCGGCTGATGG
mutable provenance: * The part was amplified from genomic DNA of E. coli str. K-12 substr. DH10B, complete genome (CP000948.1).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: start
sequence annotation: MotB coding region
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_K777117_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation2184526
display id: annotation2184526
version: 1
top level: BBa_K777117
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K777117/annotation2184526/range2184526/1

https://synbiohub.org/public/igem/BBa_K777117/annotation2184526/range2184526/1
type: Range
persistent identity: range2184526
display id: range2184526
version: 1
direction: direction/forward
top level: BBa_K777117
start: 1
end: 1
orientation: inline

MotB coding region
type: SequenceAnnotation
persistent identity: annotation2184525
display id: annotation2184525
version: 1
top level: BBa_K777117
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K777117/annotation2184525/range2184525/1

https://synbiohub.org/public/igem/BBa_K777117/annotation2184525/range2184525/1
type: Range
persistent identity: range2184525
display id: range2184525
version: 1
direction: direction/forward
top level: BBa_K777117
start: 1
end: 927
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2184527
display id: annotation2184527
version: 1
top level: BBa_K777117
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K777117/annotation2184527/range2184527/1

https://synbiohub.org/public/igem/BBa_K777117/annotation2184527/range2184527/1
type: Range
persistent identity: range2184527
display id: range2184527
version: 1
direction: direction/forward
top level: BBa_K777117
start: 927
end: 927
orientation: inline

BBa_I6076
type: ComponentDefinition
persistent identity: BBa I6076
display id: BBa_I6076
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter O_H Test R0065.E0432
creator: Caitlin Conboy and Jennifer Braff
created: 2004-03-17T12:00:00Z
modified: 2015-08-31T04:07:43Z
discontinued: false
dominant: true
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_B0012
component: BBa_B0034
component: BBa_B0010
component: BBa_R0065
component: BBa_E0032
sequence annotation: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_E0032
sequence annotation: BBa_B0012
sequence annotation: BBa_R0065
sequence: https://synbiohub.org/public/igem/BBa_I6076_sequence/1

BBa_B0012
type: Component
persistent identity: component949378
display id: component949378
version: 1
top level: BBa_I6076
access: public
definition: BBa_B0012

BBa_B0034
type: Component
persistent identity: component949348
display id: component949348
version: 1
top level: BBa_I6076
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component949368
display id: component949368
version: 1
top level: BBa_I6076
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_R0065
type: Component
persistent identity: component949336
display id: component949336
version: 1
top level: BBa_I6076
access: public
definition: cI+luxR

BBa_E0032
type: Component
persistent identity: component949360
display id: component949360
version: 1
top level: BBa_I6076
access: public
definition: YFP

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation949368
display id: annotation949368
version: 1
top level: BBa_I6076
location: https://synbiohub.org/public/igem/BBa_I6076/annotation949368/range949368/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I6076/annotation949368/range949368/1
type: Range
persistent identity: range949368
display id: range949368
version: 1
direction: direction/forward
top level: BBa_I6076
start: 894
end: 973
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation949348
display id: annotation949348
version: 1
top level: BBa_I6076
location: https://synbiohub.org/public/igem/BBa_I6076/annotation949348/range949348/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I6076/annotation949348/range949348/1
type: Range
persistent identity: range949348
display id: range949348
version: 1
direction: direction/forward
top level: BBa_I6076
start: 106
end: 117
orientation: inline

BBa_E0032
type: SequenceAnnotation
persistent identity: annotation949360
display id: annotation949360
version: 1
top level: BBa_I6076
location: https://synbiohub.org/public/igem/BBa_I6076/annotation949360/range949360/1
component: BBa_E0032

https://synbiohub.org/public/igem/BBa_I6076/annotation949360/range949360/1
type: Range
persistent identity: range949360
display id: range949360
version: 1
direction: direction/forward
top level: BBa_I6076
start: 124
end: 885
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation949378
display id: annotation949378
version: 1
top level: BBa_I6076
location: https://synbiohub.org/public/igem/BBa_I6076/annotation949378/range949378/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I6076/annotation949378/range949378/1
type: Range
persistent identity: range949378
display id: range949378
version: 1
direction: direction/forward
top level: BBa_I6076
start: 982
end: 1022
orientation: inline

BBa_R0065
type: SequenceAnnotation
persistent identity: annotation949336
display id: annotation949336
version: 1
top level: BBa_I6076
location: https://synbiohub.org/public/igem/BBa_I6076/annotation949336/range949336/1
component: BBa_R0065

https://synbiohub.org/public/igem/BBa_I6076/annotation949336/range949336/1
type: Range
persistent identity: range949336
display id: range949336
version: 1
direction: direction/forward
top level: BBa_I6076
start: 1
end: 97
orientation: inline

BBa_K801012
type: ComponentDefinition
persistent identity: BBa K801012
display id: BBa_K801012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ADH1 yeast terminator
creator: Georg Sch??tzinger
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
discontinued: false
dominant: false
group u list: _1057_
m user id: 0
owner id: 13624
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: ADH1 terminator of ''S. cerevisiae''
mutable notes: -
mutable provenance: Extracted from genomic DNA of ''S. cerevisiae'' using PCR.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: ADH1-Terminator
sequence: https://synbiohub.org/public/igem/BBa_K801012_sequence/1

ADH1-Terminator
type: SequenceAnnotation
persistent identity: annotation2202145
display id: annotation2202145
version: 1
top level: BBa_K801012
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K801012/annotation2202145/range2202145/1

https://synbiohub.org/public/igem/BBa_K801012/annotation2202145/range2202145/1
type: Range
persistent identity: range2202145
display id: range2202145
version: 1
direction: direction/forward
top level: BBa_K801012
start: 1
end: 349
orientation: inline

LasR+PAI
type: ComponentDefinition
persistent identity: BBa R0079
display id: BBa_R0079
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter (LasR & PAI regulated)
creator: Alvin Carter Powers (Fighting Darwins)
created: 2004-01-27T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Binding region for LasR protein (positive regulation)
mutable provenance: "Analysis of the Pseudomonas aeruginosa Elastase (lasB) Regulatory Region". Lynn Rust, Everett Pesci, and Barbara Iglewski
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: OP2
sequence annotation: -35
sequence annotation: -10
sequence annotation: OP1
sequence: https://synbiohub.org/public/igem/BBa_R0079_sequence/1

OP2
type: SequenceAnnotation
persistent identity: annotation300985
display id: annotation300985
version: 1
top level: LasR+PAI
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0079/annotation300985/range300985/1

https://synbiohub.org/public/igem/BBa_R0079/annotation300985/range300985/1
type: Range
persistent identity: range300985
display id: range300985
version: 1
direction: direction/forward
top level: LasR+PAI
start: 46
end: 63
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation318495
display id: annotation318495
version: 1
top level: LasR+PAI
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0079/annotation318495/range318495/1

https://synbiohub.org/public/igem/BBa_R0079/annotation318495/range318495/1
type: Range
persistent identity: range318495
display id: range318495
version: 1
direction: direction/forward
top level: LasR+PAI
start: 117
end: 122
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation318496
display id: annotation318496
version: 1
top level: LasR+PAI
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0079/annotation318496/range318496/1

https://synbiohub.org/public/igem/BBa_R0079/annotation318496/range318496/1
type: Range
persistent identity: range318496
display id: range318496
version: 1
direction: direction/forward
top level: LasR+PAI
start: 140
end: 145
orientation: inline

OP1
type: SequenceAnnotation
persistent identity: annotation300992
display id: annotation300992
version: 1
top level: LasR+PAI
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0079/annotation300992/range300992/1

https://synbiohub.org/public/igem/BBa_R0079/annotation300992/range300992/1
type: Range
persistent identity: range300992
display id: range300992
version: 1
direction: direction/forward
top level: LasR+PAI
start: 106
end: 125
orientation: inline

BBa_S0109
type: ComponentDefinition
persistent identity: BBa S0109
display id: BBa_S0109
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0033.C0051
creator: Caitlin Conboy and Jennifer Braff
created: 2003-12-04T12:00:00Z
modified: 2015-05-08T01:14:17Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_B0033
component: BBa_C0051
sequence annotation: BBa_B0033
sequence annotation: BBa_C0051
sequence: https://synbiohub.org/public/igem/BBa_S0109_sequence/1

BBa_B0033
type: Component
persistent identity: component939856
display id: component939856
version: 1
top level: BBa_S0109
access: public
definition: BBa_B0033

BBa_C0051
type: Component
persistent identity: component939867
display id: component939867
version: 1
top level: BBa_S0109
access: public
definition: cI lam

BBa_B0033
type: SequenceAnnotation
persistent identity: annotation939856
display id: annotation939856
version: 1
top level: BBa_S0109
location: https://synbiohub.org/public/igem/BBa_S0109/annotation939856/range939856/1
component: BBa_B0033

https://synbiohub.org/public/igem/BBa_S0109/annotation939856/range939856/1
type: Range
persistent identity: range939856
display id: range939856
version: 1
direction: direction/forward
top level: BBa_S0109
start: 1
end: 11
orientation: inline

BBa_C0051
type: SequenceAnnotation
persistent identity: annotation939867
display id: annotation939867
version: 1
top level: BBa_S0109
location: https://synbiohub.org/public/igem/BBa_S0109/annotation939867/range939867/1
component: BBa_C0051

https://synbiohub.org/public/igem/BBa_S0109/annotation939867/range939867/1
type: Range
persistent identity: range939867
display id: range939867
version: 1
direction: direction/forward
top level: BBa_S0109
start: 18
end: 767
orientation: inline

BBa_E5501
type: ComponentDefinition
persistent identity: BBa E5501
display id: BBa_E5501
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0032.E0040
creator: jcbraff
created: 2005-05-16T11:00:00Z
modified: 2015-08-31T04:07:27Z
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 61
owning group id: 1
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: B0032.E0040
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_B0032
component: BBa_E0040
sequence annotation: BBa_E0040
sequence annotation: BBa_B0032
sequence: https://synbiohub.org/public/igem/BBa_E5501_sequence/1

BBa_B0032
type: Component
persistent identity: component1489098
display id: component1489098
version: 1
top level: BBa_E5501
access: public
definition: BBa_B0032

BBa_E0040
type: Component
persistent identity: component1489101
display id: component1489101
version: 1
top level: BBa_E5501
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1489101
display id: annotation1489101
version: 1
top level: BBa_E5501
location: https://synbiohub.org/public/igem/BBa_E5501/annotation1489101/range1489101/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_E5501/annotation1489101/range1489101/1
type: Range
persistent identity: range1489101
display id: range1489101
version: 1
direction: direction/forward
top level: BBa_E5501
start: 20
end: 739
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1489098
display id: annotation1489098
version: 1
top level: BBa_E5501
location: https://synbiohub.org/public/igem/BBa_E5501/annotation1489098/range1489098/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_E5501/annotation1489098/range1489098/1
type: Range
persistent identity: range1489098
display id: range1489098
version: 1
direction: direction/forward
top level: BBa_E5501
start: 1
end: 13
orientation: inline

Operon2
type: ComponentDefinition
persistent identity: BBa K808007
display id: BBa_K808007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: putative terephtalate uptake operon2
creator: Valentina Herbring, Sebastian Palluk, Andreas Schmidt
created: 2012-09-04T11:00:00Z
modified: 2015-05-08T01:13:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1065_
m user id: 0
owner id: 12829
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: putative terephtalate uptake operon2 AraProm_RBS_tctA-505_RBS_tctB-197_RBS_tphC
mutable notes: -
mutable provenance: Comamonas testosteroni KF1
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K808001
component: BBa_J61101
component: BBa_K808000
component: BBa_K808004
component: BBa_J61100
component: BBa_K808005
sequence annotation: BBa_J61101
sequence annotation: BBa_K808005
sequence annotation: BBa_K808004
sequence annotation: BBa_K808000
sequence annotation: BBa_K808001
sequence annotation: BBa_J61100
sequence: https://synbiohub.org/public/igem/BBa_K808007_sequence/1

BBa_K808001
type: Component
persistent identity: component2201312
display id: component2201312
version: 1
top level: Operon2
access: public
definition: https://synbiohub.org/public/igem/BBa_K808001/1

BBa_J61101
type: Component
persistent identity: component2201311
display id: component2201311
version: 1
top level: Operon2
access: public
definition: https://synbiohub.org/public/igem/BBa_J61101/1

BBa_K808000
type: Component
persistent identity: component2201306
display id: component2201306
version: 1
top level: Operon2
access: public
definition: Ara

BBa_K808004
type: Component
persistent identity: component2201308
display id: component2201308
version: 1
top level: Operon2
access: public
definition: tctA_505aa

BBa_J61100
type: Component
persistent identity: component2201309
display id: component2201309
version: 1
top level: Operon2
access: public
definition: https://synbiohub.org/public/igem/BBa_J61100/1

BBa_K808005
type: Component
persistent identity: component2201310
display id: component2201310
version: 1
top level: Operon2
access: public
definition: https://synbiohub.org/public/igem/BBa_K808005/1

BBa_J61100
type: Component
persistent identity: component2201307
display id: component2201307
version: 1
top level: Operon2
access: public
definition: https://synbiohub.org/public/igem/BBa_J61100/1

BBa_J61101
type: SequenceAnnotation
persistent identity: annotation2201311
display id: annotation2201311
version: 1
top level: Operon2
location: https://synbiohub.org/public/igem/BBa_K808007/annotation2201311/range2201311/1
component: BBa_J61101

https://synbiohub.org/public/igem/BBa_K808007/annotation2201311/range2201311/1
type: Range
persistent identity: range2201311
display id: range2201311
version: 1
direction: direction/reverse
top level: Operon2
start: 3382
end: 3393
orientation: inline

BBa_K808005
type: SequenceAnnotation
persistent identity: annotation2201310
display id: annotation2201310
version: 1
top level: Operon2
location: https://synbiohub.org/public/igem/BBa_K808007/annotation2201310/range2201310/1
component: BBa_K808005

https://synbiohub.org/public/igem/BBa_K808007/annotation2201310/range2201310/1
type: Range
persistent identity: range2201310
display id: range2201310
version: 1
direction: direction/reverse
top level: Operon2
start: 2780
end: 3373
orientation: inline

BBa_K808004
type: SequenceAnnotation
persistent identity: annotation2201308
display id: annotation2201308
version: 1
top level: Operon2
location: https://synbiohub.org/public/igem/BBa_K808007/annotation2201308/range2201308/1
component: BBa_K808004

https://synbiohub.org/public/igem/BBa_K808007/annotation2201308/range2201308/1
type: Range
persistent identity: range2201308
display id: range2201308
version: 1
direction: direction/reverse
top level: Operon2
start: 1236
end: 2753
orientation: inline

BBa_K808000
type: SequenceAnnotation
persistent identity: annotation2201306
display id: annotation2201306
version: 1
top level: Operon2
location: https://synbiohub.org/public/igem/BBa_K808007/annotation2201306/range2201306/1
component: BBa_K808000

https://synbiohub.org/public/igem/BBa_K808007/annotation2201306/range2201306/1
type: Range
persistent identity: range2201306
display id: range2201306
version: 1
direction: direction/reverse
top level: Operon2
start: 1
end: 1209
orientation: inline

BBa_K808001
type: SequenceAnnotation
persistent identity: annotation2201312
display id: annotation2201312
version: 1
top level: Operon2
location: https://synbiohub.org/public/igem/BBa_K808007/annotation2201312/range2201312/1
component: BBa_K808001

https://synbiohub.org/public/igem/BBa_K808007/annotation2201312/range2201312/1
type: Range
persistent identity: range2201312
display id: range2201312
version: 1
direction: direction/reverse
top level: Operon2
start: 3400
end: 4368
orientation: inline

BBa_J61100
type: SequenceAnnotation
persistent identity: annotation2201307
display id: annotation2201307
version: 1
top level: Operon2
location: https://synbiohub.org/public/igem/BBa_K808007/annotation2201307/range2201307/1
component: BBa_J61100

https://synbiohub.org/public/igem/BBa_K808007/annotation2201307/range2201307/1
type: Range
persistent identity: range2201307
display id: range2201307
version: 1
direction: direction/reverse
top level: Operon2
start: 1218
end: 1229
orientation: inline

BBa_J61100
type: SequenceAnnotation
persistent identity: annotation2201309
display id: annotation2201309
version: 1
top level: Operon2
location: https://synbiohub.org/public/igem/BBa_K808007/annotation2201309/range2201309/1
component: BBa_J61100

https://synbiohub.org/public/igem/BBa_K808007/annotation2201309/range2201309/1
type: Range
persistent identity: range2201309
display id: range2201309
version: 1
direction: direction/reverse
top level: Operon2
start: 2762
end: 2773
orientation: inline

BBa_K808033
type: ComponentDefinition
persistent identity: BBa K808033
display id: BBa_K808033
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: araC-Pbad regulated XylE - catechol 2,3-dioxygenase from P.putida with terminator
creator: Valentina Herbring, Sebastian Palluk, Andreas Schmidt
created: 2012-09-05T11:00:00Z
modified: 2015-05-08T01:13:26Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1065_
m user id: 0
owner id: 12529
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J61100
component: BBa_K316003
component: BBa_K808000
sequence annotation: BBa_J61100
sequence annotation: BBa_K316003
sequence annotation: BBa_K808000
sequence: https://synbiohub.org/public/igem/BBa_K808033_sequence/1

BBa_J61100
type: Component
persistent identity: component2201323
display id: component2201323
version: 1
top level: BBa_K808033
access: public
definition: https://synbiohub.org/public/igem/BBa_J61100/1

BBa_K316003
type: Component
persistent identity: component2201335
display id: component2201335
version: 1
top level: BBa_K808033
access: public
definition: https://synbiohub.org/public/igem/BBa_K316003/1

BBa_K808000
type: Component
persistent identity: component2201322
display id: component2201322
version: 1
top level: BBa_K808033
access: public
definition: Ara

BBa_J61100
type: SequenceAnnotation
persistent identity: annotation2201323
display id: annotation2201323
version: 1
top level: BBa_K808033
location: https://synbiohub.org/public/igem/BBa_K808033/annotation2201323/range2201323/1
component: BBa_J61100

https://synbiohub.org/public/igem/BBa_K808033/annotation2201323/range2201323/1
type: Range
persistent identity: range2201323
display id: range2201323
version: 1
direction: direction/reverse
top level: BBa_K808033
start: 1218
end: 1229
orientation: inline

BBa_K316003
type: SequenceAnnotation
persistent identity: annotation2201335
display id: annotation2201335
version: 1
top level: BBa_K808033
location: https://synbiohub.org/public/igem/BBa_K808033/annotation2201335/range2201335/1
component: BBa_K316003

https://synbiohub.org/public/igem/BBa_K808033/annotation2201335/range2201335/1
type: Range
persistent identity: range2201335
display id: range2201335
version: 1
direction: direction/reverse
top level: BBa_K808033
start: 1238
end: 2298
orientation: inline

BBa_K808000
type: SequenceAnnotation
persistent identity: annotation2201322
display id: annotation2201322
version: 1
top level: BBa_K808033
location: https://synbiohub.org/public/igem/BBa_K808033/annotation2201322/range2201322/1
component: BBa_K808000

https://synbiohub.org/public/igem/BBa_K808033/annotation2201322/range2201322/1
type: Range
persistent identity: range2201322
display id: range2201322
version: 1
direction: direction/reverse
top level: BBa_K808033
start: 1
end: 1209
orientation: inline

BBa_E5504
type: ComponentDefinition
persistent identity: BBa E5504
display id: BBa_E5504
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: R0040.B0032.E0040
creator: jcbraff
created: 2005-05-16T11:00:00Z
modified: 2015-08-31T04:07:27Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 61
owning group id: 1
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: R0040.B0032.E0040 (R0040.E5501)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0032
component: BBa_E0040
component: BBa_R0040
sequence annotation: BBa_B0032
sequence annotation: BBa_R0040
sequence annotation: BBa_E0040
sequence: https://synbiohub.org/public/igem/BBa_E5504_sequence/1

BBa_B0032
type: Component
persistent identity: component1489171
display id: component1489171
version: 1
top level: BBa_E5504
access: public
definition: BBa_B0032

BBa_E0040
type: Component
persistent identity: component1489174
display id: component1489174
version: 1
top level: BBa_E5504
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_R0040
type: Component
persistent identity: component1489160
display id: component1489160
version: 1
top level: BBa_E5504
access: public
definition: p(tetR)

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1489171
display id: annotation1489171
version: 1
top level: BBa_E5504
location: https://synbiohub.org/public/igem/BBa_E5504/annotation1489171/range1489171/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_E5504/annotation1489171/range1489171/1
type: Range
persistent identity: range1489171
display id: range1489171
version: 1
direction: direction/forward
top level: BBa_E5504
start: 63
end: 75
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1489160
display id: annotation1489160
version: 1
top level: BBa_E5504
location: https://synbiohub.org/public/igem/BBa_E5504/annotation1489160/range1489160/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_E5504/annotation1489160/range1489160/1
type: Range
persistent identity: range1489160
display id: range1489160
version: 1
direction: direction/forward
top level: BBa_E5504
start: 1
end: 54
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1489174
display id: annotation1489174
version: 1
top level: BBa_E5504
location: https://synbiohub.org/public/igem/BBa_E5504/annotation1489174/range1489174/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_E5504/annotation1489174/range1489174/1
type: Range
persistent identity: range1489174
display id: range1489174
version: 1
direction: direction/forward
top level: BBa_E5504
start: 82
end: 801
orientation: inline

BBa_I6107
type: ComponentDefinition
persistent identity: BBa I6107
display id: BBa_I6107
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter O_H Test R0079.E0430
creator: cconboy
created: 2004-05-09T11:00:00Z
modified: 2015-08-31T04:07:44Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 60
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_R0079
component: BBa_B0012
component: BBa_E0030
component: BBa_B0034
component: BBa_B0010
sequence annotation: BBa_E0030
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_R0079
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_I6107_sequence/1

BBa_R0079
type: Component
persistent identity: component949919
display id: component949919
version: 1
top level: BBa_I6107
access: public
definition: LasR+PAI

BBa_B0012
type: Component
persistent identity: component949941
display id: component949941
version: 1
top level: BBa_I6107
access: public
definition: BBa_B0012

BBa_E0030
type: Component
persistent identity: component949926
display id: component949926
version: 1
top level: BBa_I6107
access: public
definition: https://synbiohub.org/public/igem/BBa_E0030/1

BBa_B0034
type: Component
persistent identity: component949924
display id: component949924
version: 1
top level: BBa_I6107
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component949931
display id: component949931
version: 1
top level: BBa_I6107
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation949926
display id: annotation949926
version: 1
top level: BBa_I6107
location: https://synbiohub.org/public/igem/BBa_I6107/annotation949926/range949926/1
component: BBa_E0030

https://synbiohub.org/public/igem/BBa_I6107/annotation949926/range949926/1
type: Range
persistent identity: range949926
display id: range949926
version: 1
direction: direction/forward
top level: BBa_I6107
start: 184
end: 906
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation949931
display id: annotation949931
version: 1
top level: BBa_I6107
location: https://synbiohub.org/public/igem/BBa_I6107/annotation949931/range949931/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I6107/annotation949931/range949931/1
type: Range
persistent identity: range949931
display id: range949931
version: 1
direction: direction/forward
top level: BBa_I6107
start: 915
end: 994
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation949941
display id: annotation949941
version: 1
top level: BBa_I6107
location: https://synbiohub.org/public/igem/BBa_I6107/annotation949941/range949941/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I6107/annotation949941/range949941/1
type: Range
persistent identity: range949941
display id: range949941
version: 1
direction: direction/forward
top level: BBa_I6107
start: 1003
end: 1043
orientation: inline

BBa_R0079
type: SequenceAnnotation
persistent identity: annotation949919
display id: annotation949919
version: 1
top level: BBa_I6107
location: https://synbiohub.org/public/igem/BBa_I6107/annotation949919/range949919/1
component: BBa_R0079

https://synbiohub.org/public/igem/BBa_I6107/annotation949919/range949919/1
type: Range
persistent identity: range949919
display id: range949919
version: 1
direction: direction/forward
top level: BBa_I6107
start: 1
end: 157
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation949924
display id: annotation949924
version: 1
top level: BBa_I6107
location: https://synbiohub.org/public/igem/BBa_I6107/annotation949924/range949924/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I6107/annotation949924/range949924/1
type: Range
persistent identity: range949924
display id: range949924
version: 1
direction: direction/forward
top level: BBa_I6107
start: 166
end: 177
orientation: inline

BBa_I13028
type: ComponentDefinition
persistent identity: BBa I13028
display id: BBa_I13028
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cI (TP-901) QPI intermediate
creator: jenmitch
created: 2004-07-14T11:00:00Z
modified: 2015-08-31T04:07:32Z
discontinued: false
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 159
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_C0075
component: BBa_B0034
sequence annotation: BBa_C0075
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_I13028_sequence/1

BBa_C0075
type: Component
persistent identity: component939472
display id: component939472
version: 1
top level: BBa_I13028
access: public
definition: https://synbiohub.org/public/igem/BBa_C0075/1

BBa_B0034
type: Component
persistent identity: component939462
display id: component939462
version: 1
top level: BBa_I13028
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_C0075
type: SequenceAnnotation
persistent identity: annotation939472
display id: annotation939472
version: 1
top level: BBa_I13028
location: https://synbiohub.org/public/igem/BBa_I13028/annotation939472/range939472/1
component: BBa_C0075

https://synbiohub.org/public/igem/BBa_I13028/annotation939472/range939472/1
type: Range
persistent identity: range939472
display id: range939472
version: 1
direction: direction/forward
top level: BBa_I13028
start: 19
end: 597
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation939462
display id: annotation939462
version: 1
top level: BBa_I13028
location: https://synbiohub.org/public/igem/BBa_I13028/annotation939462/range939462/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13028/annotation939462/range939462/1
type: Range
persistent identity: range939462
display id: range939462
version: 1
direction: direction/forward
top level: BBa_I13028
start: 1
end: 12
orientation: inline

BBa_K777113
type: ComponentDefinition
persistent identity: BBa K777113
display id: BBa_K777113
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: motA
creator: Team Goettingen
created: 2012-09-16T11:00:00Z
modified: 2015-05-08T01:13:16Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1029_
m user id: 0
owner id: 12106
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: The motA gene codes for a part of the flagellar motor complex. MotA proteins represent the proton conducting component of this molecular machine. Thus it is necessary for flagellar rotation.
mutable notes: *''motA'' was amplified from genomic DNA of ''E. coli'' DH10B via PCR using the following primers: **Fwd: GTTTCTTCGAATTCGCGGCCGCTTCTAGATGACCGGTGGAAGCCTTGG **Rev: GTTTCTTCCTGCAGCGGCCGCTACTAGTATCATGCTTCCTCGGTTGTCG
mutable provenance: * The part was amplified from genomic DNA of E. coli str. K-12 substr. DH10B, complete genome (CP000948.1).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: stop
sequence annotation: start
sequence annotation: MotA coding sequence
sequence: https://synbiohub.org/public/igem/BBa_K777113_sequence/1

stop
type: SequenceAnnotation
persistent identity: annotation2184144
display id: annotation2184144
version: 1
top level: BBa_K777113
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K777113/annotation2184144/range2184144/1

https://synbiohub.org/public/igem/BBa_K777113/annotation2184144/range2184144/1
type: Range
persistent identity: range2184144
display id: range2184144
version: 1
direction: direction/forward
top level: BBa_K777113
start: 831
end: 831
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2184143
display id: annotation2184143
version: 1
top level: BBa_K777113
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K777113/annotation2184143/range2184143/1

https://synbiohub.org/public/igem/BBa_K777113/annotation2184143/range2184143/1
type: Range
persistent identity: range2184143
display id: range2184143
version: 1
direction: direction/forward
top level: BBa_K777113
start: 1
end: 1
orientation: inline

MotA coding sequence
type: SequenceAnnotation
persistent identity: annotation2184142
display id: annotation2184142
version: 1
top level: BBa_K777113
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K777113/annotation2184142/range2184142/1

https://synbiohub.org/public/igem/BBa_K777113/annotation2184142/range2184142/1
type: Range
persistent identity: range2184142
display id: range2184142
version: 1
direction: direction/forward
top level: BBa_K777113
start: 1
end: 831
orientation: inline

BBa_K876052
type: ComponentDefinition
persistent identity: BBa K876052
display id: BBa_K876052
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: plasR-luxI-RBS-YFP-Term-Term
creator: Leah Ferrell, Sanket Shah, Shayan Ghassemi, Zakaraya Ashour
created: 2012-10-01T11:00:00Z
modified: 2015-05-08T01:13:39Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1138_
m user id: 0
owner id: 13835
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: pLasR is off by default and can be actated by LasR. LuxI converts SAM to AHL.
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_R0079
component: BBa_C0061
component: BBa_E0432
sequence annotation: BBa_E0432
sequence annotation: BBa_R0079
sequence annotation: BBa_C0061
sequence: https://synbiohub.org/public/igem/BBa_K876052_sequence/1

BBa_R0079
type: Component
persistent identity: component2264725
display id: component2264725
version: 1
top level: BBa_K876052
access: public
definition: LasR+PAI

BBa_C0061
type: Component
persistent identity: component2264727
display id: component2264727
version: 1
top level: BBa_K876052
access: public
definition: luxI

BBa_E0432
type: Component
persistent identity: component2264743
display id: component2264743
version: 1
top level: BBa_K876052
access: public
definition: https://synbiohub.org/public/igem/BBa_E0432/1

BBa_E0432
type: SequenceAnnotation
persistent identity: annotation2264743
display id: annotation2264743
version: 1
top level: BBa_K876052
location: https://synbiohub.org/public/igem/BBa_K876052/annotation2264743/range2264743/1
component: BBa_E0432

https://synbiohub.org/public/igem/BBa_K876052/annotation2264743/range2264743/1
type: Range
persistent identity: range2264743
display id: range2264743
version: 1
direction: direction/reverse
top level: BBa_K876052
start: 815
end: 1731
orientation: inline

BBa_R0079
type: SequenceAnnotation
persistent identity: annotation2264725
display id: annotation2264725
version: 1
top level: BBa_K876052
location: https://synbiohub.org/public/igem/BBa_K876052/annotation2264725/range2264725/1
component: BBa_R0079

https://synbiohub.org/public/igem/BBa_K876052/annotation2264725/range2264725/1
type: Range
persistent identity: range2264725
display id: range2264725
version: 1
direction: direction/reverse
top level: BBa_K876052
start: 1
end: 157
orientation: inline

BBa_C0061
type: SequenceAnnotation
persistent identity: annotation2264727
display id: annotation2264727
version: 1
top level: BBa_K876052
location: https://synbiohub.org/public/igem/BBa_K876052/annotation2264727/range2264727/1
component: BBa_C0061

https://synbiohub.org/public/igem/BBa_K876052/annotation2264727/range2264727/1
type: Range
persistent identity: range2264727
display id: range2264727
version: 1
direction: direction/forward
top level: BBa_K876052
start: 164
end: 781
orientation: inline

BBa_K895002
type: ComponentDefinition
persistent identity: BBa K895002
display id: BBa_K895002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Salty_VgrG
creator: Frank Sargent
created: 2012-09-21T11:00:00Z
modified: 2015-05-08T01:13:41Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1160_
m user id: 0
owner id: 8083
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This is a coding part comprising the full open reading frame of the gene encoding the Salmonella enterica LT2 VgrG protein (also known as STM0279 or TssI). VgrG is an important component of bacterial Type VI Secretion Systems and is believed to locate at the very tip of the Type VI secretion device. Normally, therefore, this protein would be considered to be a secreted protein and to be located outside of the bacterial cell.
mutable notes: The Salmonella vgrG gene did not require any modification to meet RFC[10] standard.
mutable provenance: Amplified by PCR from Salmonella LT2 genomic DNA.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: vgrG
sequence: https://synbiohub.org/public/igem/BBa_K895002_sequence/1

vgrG
type: SequenceAnnotation
persistent identity: annotation2192729
display id: annotation2192729
version: 1
top level: BBa_K895002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K895002/annotation2192729/range2192729/1

https://synbiohub.org/public/igem/BBa_K895002/annotation2192729/range2192729/1
type: Range
persistent identity: range2192729
display id: range2192729
version: 1
direction: direction/forward
top level: BBa_K895002
start: 1
end: 2193
orientation: inline

BBa_K748003
type: ComponentDefinition
persistent identity: BBa K748003
display id: BBa_K748003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: yddV is a Diguanylate Cyclase-Genomic.
creator: Lei Qiao
created: 2012-09-14T11:00:00Z
modified: 2015-05-08T01:13:12Z
discontinued: false
dominant: false
experience: experience/None
group u list: _999_
m user id: 0
owner id: 8564
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: yddV is a Diguanylate Cyclase-Genomic. The product of gene yddV has diguanylate cyclase (DGC) activity. DGC uses 2 GTP to form a Bis-(3???-5???)-cyclic dimeric guanosine monophosphate (c-di-GMP). C-di-GMP is a global second messenger in bacteria. Biofilm formation of E.coli is manipulable by varying c-di-GMP concentrations. When the c-di-GMP level stays low, there is little biofilm and the bacteria is dispersive. High concentrations of c-di-GMP promote bacteria to form more cellulose and fimbriae, which enhances the biofilm formation and decrease the motility of bacteria.
mutable notes: nothing special
mutable provenance: de novo synthesis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K748003_sequence/1

BBa_I718013
type: ComponentDefinition
persistent identity: BBa I718013
display id: BBa_I718013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ter-lox66-rbs-dapA Coli
creator: David Bikard
created: 2007-10-25T11:00:00Z
modified: 2015-08-31T04:07:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _141_
m user id: 0
owner id: 1481
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: downstream construct of the synthetic multicellular bacterium
mutable notes: none
mutable provenance: none
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_I718016
component: BBa_B0010
component: BBa_B0030
component: BBa_B0012
component: BBa_I718004
sequence annotation: BBa_B0010
sequence annotation: BBa_I718004
sequence annotation: BBa_I718016
sequence annotation: BBa_B0012
sequence annotation: BBa_B0030
sequence: https://synbiohub.org/public/igem/BBa_I718013_sequence/1

BBa_I718016
type: Component
persistent identity: component1956314
display id: component1956314
version: 1
top level: BBa_I718013
access: public
definition: https://synbiohub.org/public/igem/BBa_I718016/1

BBa_B0010
type: Component
persistent identity: component1956308
display id: component1956308
version: 1
top level: BBa_I718013
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0030
type: Component
persistent identity: component1956316
display id: component1956316
version: 1
top level: BBa_I718013
access: public
definition: BBa_B0030

BBa_B0012
type: Component
persistent identity: component1956310
display id: component1956310
version: 1
top level: BBa_I718013
access: public
definition: BBa_B0012

BBa_I718004
type: Component
persistent identity: component1956321
display id: component1956321
version: 1
top level: BBa_I718013
access: public
definition: https://synbiohub.org/public/igem/BBa_I718004/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1956308
display id: annotation1956308
version: 1
top level: BBa_I718013
location: https://synbiohub.org/public/igem/BBa_I718013/annotation1956308/range1956308/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I718013/annotation1956308/range1956308/1
type: Range
persistent identity: range1956308
display id: range1956308
version: 1
direction: direction/forward
top level: BBa_I718013
start: 1
end: 80
orientation: inline

BBa_I718004
type: SequenceAnnotation
persistent identity: annotation1956321
display id: annotation1956321
version: 1
top level: BBa_I718013
location: https://synbiohub.org/public/igem/BBa_I718013/annotation1956321/range1956321/1
component: BBa_I718004

https://synbiohub.org/public/igem/BBa_I718013/annotation1956321/range1956321/1
type: Range
persistent identity: range1956321
display id: range1956321
version: 1
direction: direction/reverse
top level: BBa_I718013
start: 201
end: 1082
orientation: inline

BBa_I718016
type: SequenceAnnotation
persistent identity: annotation1956314
display id: annotation1956314
version: 1
top level: BBa_I718013
location: https://synbiohub.org/public/igem/BBa_I718013/annotation1956314/range1956314/1
component: BBa_I718016

https://synbiohub.org/public/igem/BBa_I718013/annotation1956314/range1956314/1
type: Range
persistent identity: range1956314
display id: range1956314
version: 1
direction: direction/reverse
top level: BBa_I718013
start: 138
end: 171
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1956310
display id: annotation1956310
version: 1
top level: BBa_I718013
location: https://synbiohub.org/public/igem/BBa_I718013/annotation1956310/range1956310/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I718013/annotation1956310/range1956310/1
type: Range
persistent identity: range1956310
display id: range1956310
version: 1
direction: direction/forward
top level: BBa_I718013
start: 89
end: 129
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1956316
display id: annotation1956316
version: 1
top level: BBa_I718013
location: https://synbiohub.org/public/igem/BBa_I718013/annotation1956316/range1956316/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_I718013/annotation1956316/range1956316/1
type: Range
persistent identity: range1956316
display id: range1956316
version: 1
direction: direction/forward
top level: BBa_I718013
start: 180
end: 194
orientation: inline

BBa_I718011
type: ComponentDefinition
persistent identity: BBa I718011
display id: BBa_I718011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: rbs-dapA coli
creator: David Bikard
created: 2007-10-25T11:00:00Z
modified: 2015-08-31T04:07:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _141_
m user id: 0
owner id: 1481
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: none
mutable notes: none
mutable provenance: none
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_B0030
component: BBa_I718004
sequence annotation: BBa_B0030
sequence annotation: BBa_I718004
sequence: https://synbiohub.org/public/igem/BBa_I718011_sequence/1

BBa_B0030
type: Component
persistent identity: component1956157
display id: component1956157
version: 1
top level: BBa_I718011
access: public
definition: BBa_B0030

BBa_I718004
type: Component
persistent identity: component1956162
display id: component1956162
version: 1
top level: BBa_I718011
access: public
definition: https://synbiohub.org/public/igem/BBa_I718004/1

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1956157
display id: annotation1956157
version: 1
top level: BBa_I718011
location: https://synbiohub.org/public/igem/BBa_I718011/annotation1956157/range1956157/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_I718011/annotation1956157/range1956157/1
type: Range
persistent identity: range1956157
display id: range1956157
version: 1
direction: direction/forward
top level: BBa_I718011
start: 1
end: 15
orientation: inline

BBa_I718004
type: SequenceAnnotation
persistent identity: annotation1956162
display id: annotation1956162
version: 1
top level: BBa_I718011
location: https://synbiohub.org/public/igem/BBa_I718011/annotation1956162/range1956162/1
component: BBa_I718004

https://synbiohub.org/public/igem/BBa_I718011/annotation1956162/range1956162/1
type: Range
persistent identity: range1956162
display id: range1956162
version: 1
direction: direction/reverse
top level: BBa_I718011
start: 22
end: 903
orientation: inline

BBa_K797000
type: ComponentDefinition
persistent identity: BBa K797000
display id: BBa_K797000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: tatABCD from E.coli
creator: Tomohiro Nobeyama
created: 2012-09-19T11:00:00Z
modified: 2015-05-08T01:13:23Z
discontinued: false
dominant: false
group u list: _1053_
m user id: 0
owner id: 11588
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part is involved in TAT protein export pathway of E.coli. The part consist of three genes expressing tatA,B,C proteins. They are the components of Tat complex on cell membrane, where protein with torA signal can go through
mutable notes: This parts have very long region so that we have to divide this parts into two regions to read sequence.
mutable provenance: They are derived from tatABCD operon in Escherichia coli???s genome.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: tatA
sequence annotation: tatB
sequence annotation: tatC
sequence annotation: tatD
sequence: https://synbiohub.org/public/igem/BBa_K797000_sequence/1

tatA
type: SequenceAnnotation
persistent identity: annotation2194797
display id: annotation2194797
version: 1
top level: BBa_K797000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K797000/annotation2194797/range2194797/1

https://synbiohub.org/public/igem/BBa_K797000/annotation2194797/range2194797/1
type: Range
persistent identity: range2194797
display id: range2194797
version: 1
direction: direction/forward
top level: BBa_K797000
start: 119
end: 389
orientation: inline

tatB
type: SequenceAnnotation
persistent identity: annotation2197721
display id: annotation2197721
version: 1
top level: BBa_K797000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K797000/annotation2197721/range2197721/1

https://synbiohub.org/public/igem/BBa_K797000/annotation2197721/range2197721/1
type: Range
persistent identity: range2197721
display id: range2197721
version: 1
direction: direction/forward
top level: BBa_K797000
start: 393
end: 907
orientation: inline

tatC
type: SequenceAnnotation
persistent identity: annotation2197737
display id: annotation2197737
version: 1
top level: BBa_K797000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K797000/annotation2197737/range2197737/1

https://synbiohub.org/public/igem/BBa_K797000/annotation2197737/range2197737/1
type: Range
persistent identity: range2197737
display id: range2197737
version: 1
direction: direction/forward
top level: BBa_K797000
start: 911
end: 1687
orientation: inline

tatD
type: SequenceAnnotation
persistent identity: annotation2197836
display id: annotation2197836
version: 1
top level: BBa_K797000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K797000/annotation2197836/range2197836/1

https://synbiohub.org/public/igem/BBa_K797000/annotation2197836/range2197836/1
type: Range
persistent identity: range2197836
display id: range2197836
version: 1
direction: direction/forward
top level: BBa_K797000
start: 1716
end: 2511
orientation: inline

BBa_K731255
type: ComponentDefinition
persistent identity: BBa K731255
display id: BBa_K731255
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Inducible araC-pBAD promoter with GFPmut3b, weak RBS
creator: Daniele Rossetto
created: 2012-08-02T11:00:00Z
modified: 2015-05-08T01:13:06Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _977_
m user id: 0
owner id: 11997
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part has been used for testing and characterization of araC-pBAD promoter (part BBa_K731200) when using a weak RBS. The part is composed of part BBa_K731200 and BBa_E0240
mutable provenance: araC-pBAD from E. coli genome, GFPmut3b is from the registry (part BBa_E0840)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_E0840
component: BBa_K731201
sequence annotation: BBa_E0840
sequence annotation: BBa_K731201
sequence: https://synbiohub.org/public/igem/BBa_K731255_sequence/1

BBa_E0840
type: Component
persistent identity: component2331150
display id: component2331150
version: 1
top level: BBa_K731255
access: public
definition: GFP genera

BBa_K731201
type: Component
persistent identity: component2331139
display id: component2331139
version: 1
top level: BBa_K731255
access: public
definition: https://synbiohub.org/public/igem/BBa_K731201/1

BBa_E0840
type: SequenceAnnotation
persistent identity: annotation2331150
display id: annotation2331150
version: 1
top level: BBa_K731255
location: https://synbiohub.org/public/igem/BBa_K731255/annotation2331150/range2331150/1
component: BBa_E0840

https://synbiohub.org/public/igem/BBa_K731255/annotation2331150/range2331150/1
type: Range
persistent identity: range2331150
display id: range2331150
version: 1
direction: direction/reverse
top level: BBa_K731255
start: 1212
end: 2089
orientation: inline

BBa_K731201
type: SequenceAnnotation
persistent identity: annotation2331139
display id: annotation2331139
version: 1
top level: BBa_K731255
location: https://synbiohub.org/public/igem/BBa_K731255/annotation2331139/range2331139/1
component: BBa_K731201

https://synbiohub.org/public/igem/BBa_K731255/annotation2331139/range2331139/1
type: Range
persistent identity: range2331139
display id: range2331139
version: 1
direction: direction/reverse
top level: BBa_K731255
start: 1
end: 1203
orientation: inline

OriTR
type: ComponentDefinition
persistent identity: BBa J01003
display id: BBa_J01003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: OriT-R (Origin of transfer for the R-plasmid nic region)
creator: Golden Bear
created: 2005-10-17T11:00:00Z
modified: 2015-08-31T04:08:11Z
discontinued: false
dominant: false
experience: experience/None
group u list: _13_
m user id: 0
owner id: 395
owning group id: 13
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: OriTR, the R plasmid nic region, is where the relaxosome nicks the plasmid and conjugative transfer by R plasmid machinery begins.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Conjugation
role: SO:0000724
sequence: https://synbiohub.org/public/igem/BBa_J01003_sequence/1

lacI
type: ComponentDefinition
persistent identity: BBa C0012
display id: BBa_C0012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: lacI repressor from E. coli (+LVA)
creator: Grace Kenney, Daniel Shen, Neelaksh Varshney, Samantha Sutton
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Coding region for the LacI protein with an LVA degradation tail and without an RBS. LacI binds to the pLac regulator <bb_part>BBa_R0010</bb_part> and PLlac01 hybrid regulator <bb_part>BBa_R0011</bb_part> and inhibits transcription. IPTG (Isopropylthiogalactoside) binds to LacI and inhibits its operation, therefore promoting transcription. A rapid degredation tail (LVA) has been added to improve the High to Low performance of this part.
mutable notes: References (unparsed) here: Elowitz, M.B., Leibler, S. A synthetic oscillatory network of transcriptional regulators. Nature 403, 335-338 (2000). [ELOW00] References (unparsed) here: Elowitz, M.B., Leibler, S. A synthetic oscillatory network of transcriptional regulators. Nature 403, 335-338 (2000). [ELOW00] Sequence taken from the repressilator of Elowitz and Leibler (2000). The obtained sequence was compared to the wild-type sequence for LacI obtained through a database search. The sequence had been modified from the wild-type in that wild-type GTG start was changed to an ATG start (note, actual ORF in E.coli has several GTG starts it would seem). The LVA tag has been added for quicker degradation. Incompatible with systems containing LacI, lactose, or IPTG.
mutable provenance: represillator of Elowitz and Leibler (2000)
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Help:Barcodes
sequence annotation: lacI-LVA
sequence annotation: LVA
sequence annotation: BBa_C0012
sequence: https://synbiohub.org/public/igem/BBa_C0012_sequence/1

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213988
display id: annotation2213988
version: 1
top level: lacI
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_C0012/annotation2213988/range2213988/1

https://synbiohub.org/public/igem/BBa_C0012/annotation2213988/range2213988/1
type: Range
persistent identity: range2213988
display id: range2213988
version: 1
direction: direction/forward
top level: lacI
start: 1129
end: 1153
orientation: inline

lacI-LVA
type: SequenceAnnotation
persistent identity: annotation1723
display id: annotation1723
version: 1
top level: lacI
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0012/annotation1723/range1723/1

https://synbiohub.org/public/igem/BBa_C0012/annotation1723/range1723/1
type: Range
persistent identity: range1723
display id: range1723
version: 1
direction: direction/forward
top level: lacI
start: 1
end: 1128
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation1722
display id: annotation1722
version: 1
top level: lacI
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_C0012/annotation1722/range1722/1

https://synbiohub.org/public/igem/BBa_C0012/annotation1722/range1722/1
type: Range
persistent identity: range1722
display id: range1722
version: 1
direction: direction/forward
top level: lacI
start: 1090
end: 1128
orientation: inline

BBa_C0012
type: SequenceAnnotation
persistent identity: annotation7031
display id: annotation7031
version: 1
top level: lacI
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_C0012/annotation7031/range7031/1

https://synbiohub.org/public/igem/BBa_C0012/annotation7031/range7031/1
type: Range
persistent identity: range7031
display id: range7031
version: 1
direction: direction/forward
top level: lacI
start: 1
end: 1128
orientation: inline

pLambda+RF
type: ComponentDefinition
persistent identity: BBa I763007
display id: BBa_I763007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: promoter lambda (cI regulated) with RFP reporter
creator: Alice Pasini
created: 2007-07-29T11:00:00Z
modified: 2015-08-31T04:08:08Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _111_
m user id: 0
owner id: 2088
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Promoter lambda - cI regulated - (R0051) with RFP (E1010) as a reporter. It can be used as a detector of the glucose metabolism.
mutable notes: Reporter
mutable provenance: Igem 2007 plates
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_I13507
component: BBa_R0051
sequence annotation: BBa_R0051
sequence annotation: BBa_I13507
sequence: https://synbiohub.org/public/igem/BBa_I763007_sequence/1

BBa_I13507
type: Component
persistent identity: component2252884
display id: component2252884
version: 1
top level: pLambda+RF
access: public
definition: https://synbiohub.org/public/igem/BBa_I13507/1

BBa_R0051
type: Component
persistent identity: component2252869
display id: component2252869
version: 1
top level: pLambda+RF
access: public
definition: https://synbiohub.org/public/igem/BBa_R0051/1

BBa_R0051
type: SequenceAnnotation
persistent identity: annotation2252869
display id: annotation2252869
version: 1
top level: pLambda+RF
location: https://synbiohub.org/public/igem/BBa_I763007/annotation2252869/range2252869/1
component: BBa_R0051

https://synbiohub.org/public/igem/BBa_I763007/annotation2252869/range2252869/1
type: Range
persistent identity: range2252869
display id: range2252869
version: 1
direction: direction/forward
top level: pLambda+RF
start: 1
end: 49
orientation: inline

BBa_I13507
type: SequenceAnnotation
persistent identity: annotation2252884
display id: annotation2252884
version: 1
top level: pLambda+RF
location: https://synbiohub.org/public/igem/BBa_I763007/annotation2252884/range2252884/1
component: BBa_I13507

https://synbiohub.org/public/igem/BBa_I763007/annotation2252884/range2252884/1
type: Range
persistent identity: range2252884
display id: range2252884
version: 1
direction: direction/reverse
top level: pLambda+RF
start: 58
end: 918
orientation: inline

BBa_K812032
type: ComponentDefinition
persistent identity: BBa K812032
display id: BBa_K812032
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: mCFP with kozak sequence for eukaryotic expression
creator: Chauris Karine
created: 2012-09-19T11:00:00Z
modified: 2015-05-08T01:13:27Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _1069_
m user id: 0
owner id: 12937
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: mCFP fused with a kozak sequence to enhance its expression in eukaryotic cells. To be used with an eukaryotic expression system such as pSC2+ (K812000) and related parts.
mutable notes: PCR and cloned in pSB1C3 using EcoRI and PstI
mutable provenance: PCR amplified with kozak sequence from DNA coming from Alfonso Jaramillo's lab
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: mCFP
sequence annotation: Kozak
sequence: https://synbiohub.org/public/igem/BBa_K812032_sequence/1

mCFP
type: SequenceAnnotation
persistent identity: annotation2187737
display id: annotation2187737
version: 1
top level: BBa_K812032
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K812032/annotation2187737/range2187737/1

https://synbiohub.org/public/igem/BBa_K812032/annotation2187737/range2187737/1
type: Range
persistent identity: range2187737
display id: range2187737
version: 1
direction: direction/forward
top level: BBa_K812032
start: 6
end: 722
orientation: inline

Kozak
type: SequenceAnnotation
persistent identity: annotation2187736
display id: annotation2187736
version: 1
top level: BBa_K812032
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K812032/annotation2187736/range2187736/1

https://synbiohub.org/public/igem/BBa_K812032/annotation2187736/range2187736/1
type: Range
persistent identity: range2187736
display id: range2187736
version: 1
direction: direction/forward
top level: BBa_K812032
start: 1
end: 5
orientation: inline

BBa_K929002
type: ComponentDefinition
persistent identity: BBa K929002
display id: BBa_K929002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: modified AID with CMV and hGH-polyA
creator: Potsdam Bioware 2012 iGEM
created: 2012-09-17T11:00:00Z
modified: 2015-05-08T01:13:46Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1194_
m user id: 0
owner id: 14457
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: The BioBrick wtAID is an extended version of the existing AID BioBrick (BBa_K929001). It is built of 3 parts: CMV promoter (BBa_I712004), superAID and hGH polyadenylation signal sequence (BBa_K404108). AID: AID is known to be responsible for somatic hypermutation and the class-switch recombination of immunoglobulin in B cells. This enzyme of 28 kDa originally occurs in B cells but does also show activity after transfection into CHO cells. AID induces the deamination of cytidine to uridine at actively transcribed single strand DNA. The replacement of cytidine by uridine leads to a mismatch during DNA replication and integrates a single base substitution predominantly in the immunoglobulin genes. The AID motif is naturally terminated with the Nuclear Export Sequence (NES) that causes the protein to translocate from the nucleus to the cytoplasm. Additionally, upstream, dysfunctional Nuclear Localization Sequence (NLS) is located. Due to the fact that AID mutates the actively transcribed single stranded DNA, it is supposed that the direction of the enzyme to the inside of the nucleus would improve the mutation rate. That is why we called it superAID Functional NLS sequence: This part of the BioBrick directs the expressed protein into the nucleus, where it can mutate stronger. Kozak sequence Kozak consensus sequence is added upstream of the AID mutant to express the protein stronger. CMV promoter: CMV stands for cytomegalovirus. The CMV promoter is commonly used due to its very strong activity, and effectivity in a broad range of cell types. The BioBrick is therefore improved via addition of the strong promoter. hGH polyadenylation signal sequence: Polyadenylation is a significant part for the translation and stability of mRNA. In eukaryotes, it is part of the process that produces mature messenger RNA (mRNA) for translation. It, therefore, forms part of the larger process of gene expression. hGH terminator gives a signal to start polyadenylation in the translation proce…
mutable notes: blaa bla
mutable provenance: 3 other Biobricks: CMV promoter (BBa_I712004), superAID (BBa_K929001) and hGH polyadenylation signal sequence (BBa_K404108).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: NLS sequence
sequence annotation: AUG
sequence annotation: AgeI
sequence annotation: BBa_K929001
sequence annotation: BBa_K404108
sequence annotation: hGH terminator
sequence annotation: CMV promoter
sequence annotation: BBa_I712004
sequence annotation: UAA
sequence annotation: Kozak sequence
sequence annotation: mod. AID
sequence: https://synbiohub.org/public/igem/BBa_K929002_sequence/1

NLS sequence
type: SequenceAnnotation
persistent identity: annotation2184471
display id: annotation2184471
version: 1
top level: BBa_K929002
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K929002/annotation2184471/range2184471/1

https://synbiohub.org/public/igem/BBa_K929002/annotation2184471/range2184471/1
type: Range
persistent identity: range2184471
display id: range2184471
version: 1
direction: direction/forward
top level: BBa_K929002
start: 678
end: 695
orientation: inline

AUG
type: SequenceAnnotation
persistent identity: annotation2193793
display id: annotation2193793
version: 1
top level: BBa_K929002
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K929002/annotation2193793/range2193793/1

https://synbiohub.org/public/igem/BBa_K929002/annotation2193793/range2193793/1
type: Range
persistent identity: range2193793
display id: range2193793
version: 1
direction: direction/forward
top level: BBa_K929002
start: 671
end: 673
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2196257
display id: annotation2196257
version: 1
top level: BBa_K929002
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K929002/annotation2196257/range2196257/1

https://synbiohub.org/public/igem/BBa_K929002/annotation2196257/range2196257/1
type: Range
persistent identity: range2196257
display id: range2196257
version: 1
direction: direction/forward
top level: BBa_K929002
start: 1241
end: 1246
orientation: inline

BBa_K929001
type: SequenceAnnotation
persistent identity: annotation2184473
display id: annotation2184473
version: 1
top level: BBa_K929002
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K929002/annotation2184473/range2184473/1

https://synbiohub.org/public/igem/BBa_K929002/annotation2184473/range2184473/1
type: Range
persistent identity: range2184473
display id: range2184473
version: 1
direction: direction/forward
top level: BBa_K929002
start: 663
end: 1249
orientation: inline

BBa_K404108
type: SequenceAnnotation
persistent identity: annotation2184475
display id: annotation2184475
version: 1
top level: BBa_K929002
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K929002/annotation2184475/range2184475/1

https://synbiohub.org/public/igem/BBa_K929002/annotation2184475/range2184475/1
type: Range
persistent identity: range2184475
display id: range2184475
version: 1
direction: direction/forward
top level: BBa_K929002
start: 1259
end: 1736
orientation: inline

hGH terminator
type: SequenceAnnotation
persistent identity: annotation2184474
display id: annotation2184474
version: 1
top level: BBa_K929002
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_K929002/annotation2184474/range2184474/1

https://synbiohub.org/public/igem/BBa_K929002/annotation2184474/range2184474/1
type: Range
persistent identity: range2184474
display id: range2184474
version: 1
direction: direction/forward
top level: BBa_K929002
start: 1259
end: 1736
orientation: inline

CMV promoter
type: SequenceAnnotation
persistent identity: annotation2184468
display id: annotation2184468
version: 1
top level: BBa_K929002
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K929002/annotation2184468/range2184468/1

https://synbiohub.org/public/igem/BBa_K929002/annotation2184468/range2184468/1
type: Range
persistent identity: range2184468
display id: range2184468
version: 1
direction: direction/forward
top level: BBa_K929002
start: 1
end: 654
orientation: inline

BBa_I712004
type: SequenceAnnotation
persistent identity: annotation2184469
display id: annotation2184469
version: 1
top level: BBa_K929002
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K929002/annotation2184469/range2184469/1

https://synbiohub.org/public/igem/BBa_K929002/annotation2184469/range2184469/1
type: Range
persistent identity: range2184469
display id: range2184469
version: 1
direction: direction/forward
top level: BBa_K929002
start: 1
end: 654
orientation: inline

UAA
type: SequenceAnnotation
persistent identity: annotation2193794
display id: annotation2193794
version: 1
top level: BBa_K929002
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K929002/annotation2193794/range2193794/1

https://synbiohub.org/public/igem/BBa_K929002/annotation2193794/range2193794/1
type: Range
persistent identity: range2193794
display id: range2193794
version: 1
direction: direction/forward
top level: BBa_K929002
start: 1247
end: 1249
orientation: inline

Kozak sequence
type: SequenceAnnotation
persistent identity: annotation2184470
display id: annotation2184470
version: 1
top level: BBa_K929002
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K929002/annotation2184470/range2184470/1

https://synbiohub.org/public/igem/BBa_K929002/annotation2184470/range2184470/1
type: Range
persistent identity: range2184470
display id: range2184470
version: 1
direction: direction/forward
top level: BBa_K929002
start: 663
end: 677
orientation: inline

mod. AID
type: SequenceAnnotation
persistent identity: annotation2184472
display id: annotation2184472
version: 1
top level: BBa_K929002
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K929002/annotation2184472/range2184472/1

https://synbiohub.org/public/igem/BBa_K929002/annotation2184472/range2184472/1
type: Range
persistent identity: range2184472
display id: range2184472
version: 1
direction: direction/forward
top level: BBa_K929002
start: 696
end: 1250
orientation: inline

BBa_S01003
type: ComponentDefinition
persistent identity: BBa S01003
display id: BBa_S01003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: R0062.E0422
creator: Randy Rettberg
created: 2005-06-10T11:00:00Z
modified: 2015-05-08T01:14:16Z
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 25
owning group id: 1
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Intermediate part from Jen 1 tube 45
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_R0062
component: BBa_B0010
component: BBa_E0022
component: BBa_B0034
component: BBa_B0012
sequence annotation: BBa_R0062
sequence annotation: BBa_E0022
sequence annotation: BBa_B0034
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_S01003_sequence/1

BBa_R0062
type: Component
persistent identity: component1510974
display id: component1510974
version: 1
top level: BBa_S01003
access: public
definition: lux pR

BBa_B0010
type: Component
persistent identity: component1511002
display id: component1511002
version: 1
top level: BBa_S01003
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_E0022
type: Component
persistent identity: component1510994
display id: component1510994
version: 1
top level: BBa_S01003
access: public
definition: ECFP

BBa_B0034
type: Component
persistent identity: component1510982
display id: component1510982
version: 1
top level: BBa_S01003
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: Component
persistent identity: component1511012
display id: component1511012
version: 1
top level: BBa_S01003
access: public
definition: BBa_B0012

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation1510974
display id: annotation1510974
version: 1
top level: BBa_S01003
location: https://synbiohub.org/public/igem/BBa_S01003/annotation1510974/range1510974/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_S01003/annotation1510974/range1510974/1
type: Range
persistent identity: range1510974
display id: range1510974
version: 1
direction: direction/forward
top level: BBa_S01003
start: 1
end: 55
orientation: inline

BBa_E0022
type: SequenceAnnotation
persistent identity: annotation1510994
display id: annotation1510994
version: 1
top level: BBa_S01003
location: https://synbiohub.org/public/igem/BBa_S01003/annotation1510994/range1510994/1
component: BBa_E0022

https://synbiohub.org/public/igem/BBa_S01003/annotation1510994/range1510994/1
type: Range
persistent identity: range1510994
display id: range1510994
version: 1
direction: direction/forward
top level: BBa_S01003
start: 82
end: 843
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1510982
display id: annotation1510982
version: 1
top level: BBa_S01003
location: https://synbiohub.org/public/igem/BBa_S01003/annotation1510982/range1510982/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_S01003/annotation1510982/range1510982/1
type: Range
persistent identity: range1510982
display id: range1510982
version: 1
direction: direction/forward
top level: BBa_S01003
start: 64
end: 75
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1511012
display id: annotation1511012
version: 1
top level: BBa_S01003
location: https://synbiohub.org/public/igem/BBa_S01003/annotation1511012/range1511012/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_S01003/annotation1511012/range1511012/1
type: Range
persistent identity: range1511012
display id: range1511012
version: 1
direction: direction/forward
top level: BBa_S01003
start: 940
end: 980
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1511002
display id: annotation1511002
version: 1
top level: BBa_S01003
location: https://synbiohub.org/public/igem/BBa_S01003/annotation1511002/range1511002/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_S01003/annotation1511002/range1511002/1
type: Range
persistent identity: range1511002
display id: range1511002
version: 1
direction: direction/forward
top level: BBa_S01003
start: 852
end: 931
orientation: inline

BBa_K844007
type: ComponentDefinition
persistent identity: BBa K844007
display id: BBa_K844007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Spider Silk 1x Subunit "F" (Fewest tRNA codon optimized) with Met (ATG) added
creator: Charles Barentine
created: 2012-10-01T11:00:00Z
modified: 2015-05-08T01:13:33Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1104_
m user id: 0
owner id: 10131
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Spider silk subunit optimized to use the fewest number of tRNA codons; contains two elasticity domains and one strength domain, also contains 5??? Met (ATG) codon.
mutable notes: none
mutable provenance: Created using mutagenesis PCR to add upstream Met (ATG) to part BBa_K844003
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Beta-spiral
sequence annotation: Elasticity Domain
sequence annotation: Linker Domain
sequence annotation: Start
sequence annotation: Beta-helix
sequence annotation: Strength Domain
sequence: https://synbiohub.org/public/igem/BBa_K844007_sequence/1

Beta-spiral
type: SequenceAnnotation
persistent identity: annotation2212476
display id: annotation2212476
version: 1
top level: BBa_K844007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K844007/annotation2212476/range2212476/1

https://synbiohub.org/public/igem/BBa_K844007/annotation2212476/range2212476/1
type: Range
persistent identity: range2212476
display id: range2212476
version: 1
direction: direction/forward
top level: BBa_K844007
start: 117
end: 132
orientation: inline

Elasticity Domain
type: SequenceAnnotation
persistent identity: annotation2212467
display id: annotation2212467
version: 1
top level: BBa_K844007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K844007/annotation2212467/range2212467/1

https://synbiohub.org/public/igem/BBa_K844007/annotation2212467/range2212467/1
type: Range
persistent identity: range2212467
display id: range2212467
version: 1
direction: direction/forward
top level: BBa_K844007
start: 4
end: 87
orientation: inline

Linker Domain
type: SequenceAnnotation
persistent identity: annotation2212479
display id: annotation2212479
version: 1
top level: BBa_K844007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K844007/annotation2212479/range2212479/1

https://synbiohub.org/public/igem/BBa_K844007/annotation2212479/range2212479/1
type: Range
persistent identity: range2212479
display id: range2212479
version: 1
direction: direction/forward
top level: BBa_K844007
start: 172
end: 188
orientation: inline

Elasticity Domain
type: SequenceAnnotation
persistent identity: annotation2212473
display id: annotation2212473
version: 1
top level: BBa_K844007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K844007/annotation2212473/range2212473/1

https://synbiohub.org/public/igem/BBa_K844007/annotation2212473/range2212473/1
type: Range
persistent identity: range2212473
display id: range2212473
version: 1
direction: direction/forward
top level: BBa_K844007
start: 88
end: 171
orientation: inline

Start
type: SequenceAnnotation
persistent identity: annotation2212466
display id: annotation2212466
version: 1
top level: BBa_K844007
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K844007/annotation2212466/range2212466/1

https://synbiohub.org/public/igem/BBa_K844007/annotation2212466/range2212466/1
type: Range
persistent identity: range2212466
display id: range2212466
version: 1
direction: direction/forward
top level: BBa_K844007
start: 1
end: 3
orientation: inline

Beta-spiral
type: SequenceAnnotation
persistent identity: annotation2212478
display id: annotation2212478
version: 1
top level: BBa_K844007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K844007/annotation2212478/range2212478/1

https://synbiohub.org/public/igem/BBa_K844007/annotation2212478/range2212478/1
type: Range
persistent identity: range2212478
display id: range2212478
version: 1
direction: direction/forward
top level: BBa_K844007
start: 156
end: 171
orientation: inline

Beta-helix
type: SequenceAnnotation
persistent identity: annotation2212468
display id: annotation2212468
version: 1
top level: BBa_K844007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K844007/annotation2212468/range2212468/1

https://synbiohub.org/public/igem/BBa_K844007/annotation2212468/range2212468/1
type: Range
persistent identity: range2212468
display id: range2212468
version: 1
direction: direction/forward
top level: BBa_K844007
start: 4
end: 12
orientation: inline

Beta-spiral
type: SequenceAnnotation
persistent identity: annotation2212475
display id: annotation2212475
version: 1
top level: BBa_K844007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K844007/annotation2212475/range2212475/1

https://synbiohub.org/public/igem/BBa_K844007/annotation2212475/range2212475/1
type: Range
persistent identity: range2212475
display id: range2212475
version: 1
direction: direction/forward
top level: BBa_K844007
start: 98
end: 113
orientation: inline

Strength Domain
type: SequenceAnnotation
persistent identity: annotation2212480
display id: annotation2212480
version: 1
top level: BBa_K844007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K844007/annotation2212480/range2212480/1

https://synbiohub.org/public/igem/BBa_K844007/annotation2212480/range2212480/1
type: Range
persistent identity: range2212480
display id: range2212480
version: 1
direction: direction/forward
top level: BBa_K844007
start: 189
end: 207
orientation: inline

Beta-helix
type: SequenceAnnotation
persistent identity: annotation2212474
display id: annotation2212474
version: 1
top level: BBa_K844007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K844007/annotation2212474/range2212474/1

https://synbiohub.org/public/igem/BBa_K844007/annotation2212474/range2212474/1
type: Range
persistent identity: range2212474
display id: range2212474
version: 1
direction: direction/forward
top level: BBa_K844007
start: 88
end: 97
orientation: inline

Beta-spiral
type: SequenceAnnotation
persistent identity: annotation2212470
display id: annotation2212470
version: 1
top level: BBa_K844007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K844007/annotation2212470/range2212470/1

https://synbiohub.org/public/igem/BBa_K844007/annotation2212470/range2212470/1
type: Range
persistent identity: range2212470
display id: range2212470
version: 1
direction: direction/forward
top level: BBa_K844007
start: 34
end: 48
orientation: inline

Beta-spiral
type: SequenceAnnotation
persistent identity: annotation2212471
display id: annotation2212471
version: 1
top level: BBa_K844007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K844007/annotation2212471/range2212471/1

https://synbiohub.org/public/igem/BBa_K844007/annotation2212471/range2212471/1
type: Range
persistent identity: range2212471
display id: range2212471
version: 1
direction: direction/forward
top level: BBa_K844007
start: 49
end: 63
orientation: inline

Beta-spiral
type: SequenceAnnotation
persistent identity: annotation2212477
display id: annotation2212477
version: 1
top level: BBa_K844007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K844007/annotation2212477/range2212477/1

https://synbiohub.org/public/igem/BBa_K844007/annotation2212477/range2212477/1
type: Range
persistent identity: range2212477
display id: range2212477
version: 1
direction: direction/forward
top level: BBa_K844007
start: 133
end: 147
orientation: inline

Beta-spiral
type: SequenceAnnotation
persistent identity: annotation2212469
display id: annotation2212469
version: 1
top level: BBa_K844007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K844007/annotation2212469/range2212469/1

https://synbiohub.org/public/igem/BBa_K844007/annotation2212469/range2212469/1
type: Range
persistent identity: range2212469
display id: range2212469
version: 1
direction: direction/forward
top level: BBa_K844007
start: 13
end: 27
orientation: inline

Beta-spiral
type: SequenceAnnotation
persistent identity: annotation2212472
display id: annotation2212472
version: 1
top level: BBa_K844007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K844007/annotation2212472/range2212472/1

https://synbiohub.org/public/igem/BBa_K844007/annotation2212472/range2212472/1
type: Range
persistent identity: range2212472
display id: range2212472
version: 1
direction: direction/forward
top level: BBa_K844007
start: 72
end: 87
orientation: inline

BBa_K737005
type: ComponentDefinition
persistent identity: BBa K737005
display id: BBa_K737005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Inducible GVPC Generator
creator: Li Kang
created: 2012-09-17T11:00:00Z
modified: 2015-05-08T01:13:07Z
discontinued: false
dominant: false
experience: experience/None
group u list: _986_
m user id: 0
owner id: 14291
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part contains a constitutive promoter (J23106), a coding region of TetR protein, a terminator (B0015), a promoter repressed by TetR protein (R0040) a coding region of GvpC and GFP and a terminator (B0015) behind it. This part can be used to induce the strength of promoter (R0040) by Tet and indicate the content of GvpC by fluorescence of GFP. In short, it can regulate the expression of GvpC. We can use different concentration of Tet to regulate the strength of promoter (R0040) ahead GvpC Protein and GFP. And We can know the approximate concentration of GvpC by the fluorescence unit of GFP. To achieve this goal, we measured a curve to connect the concentration of IPTG and the fluorescence unit of GFP. Here is the curve:
mutable notes: We can use different concentration of Tet to regulate the strength of promoter (R0040) ahead GvpC Protein and GFP. And We can know the approximate concentration of GvpC by the fluorescence unit of GFP. To achieve this goal, we measured a curve to connect the concentration of IPTG and the fluorescence unit of GFP.
mutable provenance: No
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_R0040
component: BBa_B0034
component: BBa_P0440
component: BBa_E0840
component: BBa_K737017
component: BBa_J23106
sequence annotation: BBa_P0440
sequence annotation: BBa_E0840
sequence annotation: BBa_R0040
sequence annotation: BBa_J23106
sequence annotation: BBa_B0034
sequence annotation: BBa_K737017
sequence: https://synbiohub.org/public/igem/BBa_K737005_sequence/1

BBa_R0040
type: Component
persistent identity: component2184854
display id: component2184854
version: 1
top level: BBa_K737005
access: public
definition: p(tetR)

BBa_B0034
type: Component
persistent identity: component2184860
display id: component2184860
version: 1
top level: BBa_K737005
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_P0440
type: Component
persistent identity: component2184853
display id: component2184853
version: 1
top level: BBa_K737005
access: public
definition: BBa_P0440

BBa_E0840
type: Component
persistent identity: component2184872
display id: component2184872
version: 1
top level: BBa_K737005
access: public
definition: GFP genera

BBa_K737017
type: Component
persistent identity: component2184861
display id: component2184861
version: 1
top level: BBa_K737005
access: public
definition: https://synbiohub.org/public/igem/BBa_K737017/1

BBa_J23106
type: Component
persistent identity: component2184840
display id: component2184840
version: 1
top level: BBa_K737005
access: public
definition: https://synbiohub.org/public/igem/BBa_J23106/1

BBa_P0440
type: SequenceAnnotation
persistent identity: annotation2184853
display id: annotation2184853
version: 1
top level: BBa_K737005
location: https://synbiohub.org/public/igem/BBa_K737005/annotation2184853/range2184853/1
component: BBa_P0440

https://synbiohub.org/public/igem/BBa_K737005/annotation2184853/range2184853/1
type: Range
persistent identity: range2184853
display id: range2184853
version: 1
direction: direction/reverse
top level: BBa_K737005
start: 44
end: 883
orientation: inline

BBa_E0840
type: SequenceAnnotation
persistent identity: annotation2184872
display id: annotation2184872
version: 1
top level: BBa_K737005
location: https://synbiohub.org/public/igem/BBa_K737005/annotation2184872/range2184872/1
component: BBa_E0840

https://synbiohub.org/public/igem/BBa_K737005/annotation2184872/range2184872/1
type: Range
persistent identity: range2184872
display id: range2184872
version: 1
direction: direction/reverse
top level: BBa_K737005
start: 1484
end: 2361
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2184854
display id: annotation2184854
version: 1
top level: BBa_K737005
location: https://synbiohub.org/public/igem/BBa_K737005/annotation2184854/range2184854/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K737005/annotation2184854/range2184854/1
type: Range
persistent identity: range2184854
display id: range2184854
version: 1
direction: direction/forward
top level: BBa_K737005
start: 892
end: 945
orientation: inline

BBa_J23106
type: SequenceAnnotation
persistent identity: annotation2184840
display id: annotation2184840
version: 1
top level: BBa_K737005
location: https://synbiohub.org/public/igem/BBa_K737005/annotation2184840/range2184840/1
component: BBa_J23106

https://synbiohub.org/public/igem/BBa_K737005/annotation2184840/range2184840/1
type: Range
persistent identity: range2184840
display id: range2184840
version: 1
direction: direction/reverse
top level: BBa_K737005
start: 1
end: 35
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2184860
display id: annotation2184860
version: 1
top level: BBa_K737005
location: https://synbiohub.org/public/igem/BBa_K737005/annotation2184860/range2184860/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K737005/annotation2184860/range2184860/1
type: Range
persistent identity: range2184860
display id: range2184860
version: 1
direction: direction/reverse
top level: BBa_K737005
start: 954
end: 965
orientation: inline

BBa_K737017
type: SequenceAnnotation
persistent identity: annotation2184861
display id: annotation2184861
version: 1
top level: BBa_K737005
location: https://synbiohub.org/public/igem/BBa_K737005/annotation2184861/range2184861/1
component: BBa_K737017

https://synbiohub.org/public/igem/BBa_K737005/annotation2184861/range2184861/1
type: Range
persistent identity: range2184861
display id: range2184861
version: 1
direction: direction/reverse
top level: BBa_K737005
start: 972
end: 1475
orientation: inline

BBa_K773004
type: ComponentDefinition
persistent identity: BBa K773004
display id: BBa_K773004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: mCherry-AAV
creator: Katie Knister
created: 2012-09-30T11:00:00Z
modified: 2015-05-08T01:13:15Z
discontinued: false
dominant: false
group u list: _1025_
m user id: 0
owner id: 11646
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part holds the fluorescent protein mCherry attached to a degradation tag AAV which breaks down fluorescent protein over time.
mutable notes: We eliminated a restriction site from the gene.
mutable provenance: This part was modified from a plasmid provided by the Murray lab at Caltech.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: ssrA degradation tag: AAV
sequence annotation: Stop
sequence annotation: B0034
sequence annotation: Start
sequence annotation: mCherry-AAV
sequence: https://synbiohub.org/public/igem/BBa_K773004_sequence/1

ssrA degradation tag: AAV
type: SequenceAnnotation
persistent identity: annotation2209506
display id: annotation2209506
version: 1
top level: BBa_K773004
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K773004/annotation2209506/range2209506/1

https://synbiohub.org/public/igem/BBa_K773004/annotation2209506/range2209506/1
type: Range
persistent identity: range2209506
display id: range2209506
version: 1
direction: direction/forward
top level: BBa_K773004
start: 730
end: 759
orientation: inline

Stop
type: SequenceAnnotation
persistent identity: annotation2209505
display id: annotation2209505
version: 1
top level: BBa_K773004
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K773004/annotation2209505/range2209505/1

https://synbiohub.org/public/igem/BBa_K773004/annotation2209505/range2209505/1
type: Range
persistent identity: range2209505
display id: range2209505
version: 1
direction: direction/forward
top level: BBa_K773004
start: 763
end: 765
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2209502
display id: annotation2209502
version: 1
top level: BBa_K773004
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K773004/annotation2209502/range2209502/1

https://synbiohub.org/public/igem/BBa_K773004/annotation2209502/range2209502/1
type: Range
persistent identity: range2209502
display id: range2209502
version: 1
direction: direction/forward
top level: BBa_K773004
start: 1
end: 12
orientation: inline

Start
type: SequenceAnnotation
persistent identity: annotation2209507
display id: annotation2209507
version: 1
top level: BBa_K773004
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K773004/annotation2209507/range2209507/1

https://synbiohub.org/public/igem/BBa_K773004/annotation2209507/range2209507/1
type: Range
persistent identity: range2209507
display id: range2209507
version: 1
direction: direction/forward
top level: BBa_K773004
start: 19
end: 21
orientation: inline

mCherry-AAV
type: SequenceAnnotation
persistent identity: annotation2209503
display id: annotation2209503
version: 1
top level: BBa_K773004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K773004/annotation2209503/range2209503/1

https://synbiohub.org/public/igem/BBa_K773004/annotation2209503/range2209503/1
type: Range
persistent identity: range2209503
display id: range2209503
version: 1
direction: direction/forward
top level: BBa_K773004
start: 19
end: 765
orientation: inline

Stop
type: SequenceAnnotation
persistent identity: annotation2209504
display id: annotation2209504
version: 1
top level: BBa_K773004
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K773004/annotation2209504/range2209504/1

https://synbiohub.org/public/igem/BBa_K773004/annotation2209504/range2209504/1
type: Range
persistent identity: range2209504
display id: range2209504
version: 1
direction: direction/forward
top level: BBa_K773004
start: 760
end: 762
orientation: inline

BBa_J04421
type: ComponentDefinition
persistent identity: BBa J04421
display id: BBa_J04421
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ECFP Coding Device with Promoter, RBS, Coding Sequence, and Terminator
creator: Kristen DeCelle
created: 2005-06-08T11:00:00Z
modified: 2016-10-14T07:25:32Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _16_
m user id: 31248
owner id: 326
owning group id: 16
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: IPTG Inducible Promoter, RBS, ECFP with LVA tag, Terminator
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0034
component: BBa_B0010
component: BBa_E0022
component: BBa_B0012
component: BBa_R0010
sequence annotation: BBa_B0034
sequence annotation: BBa_B0010
sequence annotation: BBa_E0022
sequence annotation: BBa_B0012
sequence annotation: BBa_R0010
sequence: https://synbiohub.org/public/igem/BBa_J04421_sequence/1

BBa_B0034
type: Component
persistent identity: component1509508
display id: component1509508
version: 1
top level: BBa_J04421
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component1509528
display id: component1509528
version: 1
top level: BBa_J04421
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_E0022
type: Component
persistent identity: component1509520
display id: component1509520
version: 1
top level: BBa_J04421
access: public
definition: ECFP

BBa_B0012
type: Component
persistent identity: component1509538
display id: component1509538
version: 1
top level: BBa_J04421
access: public
definition: BBa_B0012

BBa_R0010
type: Component
persistent identity: component1509498
display id: component1509498
version: 1
top level: BBa_J04421
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1509508
display id: annotation1509508
version: 1
top level: BBa_J04421
location: https://synbiohub.org/public/igem/BBa_J04421/annotation1509508/range1509508/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J04421/annotation1509508/range1509508/1
type: Range
persistent identity: range1509508
display id: range1509508
version: 1
direction: direction/forward
top level: BBa_J04421
start: 209
end: 220
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1509528
display id: annotation1509528
version: 1
top level: BBa_J04421
location: https://synbiohub.org/public/igem/BBa_J04421/annotation1509528/range1509528/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J04421/annotation1509528/range1509528/1
type: Range
persistent identity: range1509528
display id: range1509528
version: 1
direction: direction/forward
top level: BBa_J04421
start: 997
end: 1076
orientation: inline

BBa_E0022
type: SequenceAnnotation
persistent identity: annotation1509520
display id: annotation1509520
version: 1
top level: BBa_J04421
location: https://synbiohub.org/public/igem/BBa_J04421/annotation1509520/range1509520/1
component: BBa_E0022

https://synbiohub.org/public/igem/BBa_J04421/annotation1509520/range1509520/1
type: Range
persistent identity: range1509520
display id: range1509520
version: 1
direction: direction/forward
top level: BBa_J04421
start: 227
end: 988
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1509538
display id: annotation1509538
version: 1
top level: BBa_J04421
location: https://synbiohub.org/public/igem/BBa_J04421/annotation1509538/range1509538/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J04421/annotation1509538/range1509538/1
type: Range
persistent identity: range1509538
display id: range1509538
version: 1
direction: direction/forward
top level: BBa_J04421
start: 1085
end: 1125
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1509498
display id: annotation1509498
version: 1
top level: BBa_J04421
location: https://synbiohub.org/public/igem/BBa_J04421/annotation1509498/range1509498/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_J04421/annotation1509498/range1509498/1
type: Range
persistent identity: range1509498
display id: range1509498
version: 1
direction: direction/forward
top level: BBa_J04421
start: 1
end: 200
orientation: inline

BBa_J37019
type: ComponentDefinition
persistent identity: BBa J37019
display id: BBa_J37019
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AHL induced LuxR generator
creator: Deepti Aswani
created: 2006-07-18T11:00:00Z
modified: 2015-08-31T04:08:48Z
discontinued: false
dominant: false
group u list: _66_
m user id: 0
owner id: 1064
owning group id: 66
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_C0062
component: BBa_R0062
component: BBa_B0034
sequence annotation: BBa_R0062
sequence annotation: BBa_B0034
sequence annotation: BBa_C0062
sequence: https://synbiohub.org/public/igem/BBa_J37019_sequence/1

BBa_C0062
type: Component
persistent identity: component2245223
display id: component2245223
version: 1
top level: BBa_J37019
access: public
definition: luxr

BBa_R0062
type: Component
persistent identity: component2245214
display id: component2245214
version: 1
top level: BBa_J37019
access: public
definition: lux pR

BBa_B0034
type: Component
persistent identity: component2245220
display id: component2245220
version: 1
top level: BBa_J37019
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation2245214
display id: annotation2245214
version: 1
top level: BBa_J37019
location: https://synbiohub.org/public/igem/BBa_J37019/annotation2245214/range2245214/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_J37019/annotation2245214/range2245214/1
type: Range
persistent identity: range2245214
display id: range2245214
version: 1
direction: direction/forward
top level: BBa_J37019
start: 1
end: 55
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2245220
display id: annotation2245220
version: 1
top level: BBa_J37019
location: https://synbiohub.org/public/igem/BBa_J37019/annotation2245220/range2245220/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J37019/annotation2245220/range2245220/1
type: Range
persistent identity: range2245220
display id: range2245220
version: 1
direction: direction/reverse
top level: BBa_J37019
start: 64
end: 75
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation2245223
display id: annotation2245223
version: 1
top level: BBa_J37019
location: https://synbiohub.org/public/igem/BBa_J37019/annotation2245223/range2245223/1
component: BBa_C0062

https://synbiohub.org/public/igem/BBa_J37019/annotation2245223/range2245223/1
type: Range
persistent identity: range2245223
display id: range2245223
version: 1
direction: direction/forward
top level: BBa_J37019
start: 82
end: 837
orientation: inline

BBa_K737051
type: ComponentDefinition
persistent identity: BBa K737051
display id: BBa_K737051
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: IPTG inducible GFP Generator
creator: Weihong Lai and Peiran Zhang
created: 2012-09-18T11:00:00Z
modified: 2015-05-08T01:13:08Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _986_
m user id: 0
owner id: 14291
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: [J23106,P0412]is a constitutive lacI repressor generator,Fluorescence can be observed with induced by IPTG. The RBS of E0240 is B0030,a weak GFP translational unit specialized to quantitively describe the behaviour of promotor in many cases. NOTE: R0011 don???t work well in high copy number plasmids.
mutable notes: No
mutable provenance: No
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_R0011
component: BBa_E0240
component: BBa_J23106
component: BBa_P0412
sequence annotation: BBa_E0240
sequence annotation: BBa_R0011
sequence annotation: BBa_P0412
sequence annotation: BBa_J23106
sequence: https://synbiohub.org/public/igem/BBa_K737051_sequence/1

BBa_R0011
type: Component
persistent identity: component2185899
display id: component2185899
version: 1
top level: BBa_K737051
access: public
definition: lacI+pL

BBa_E0240
type: Component
persistent identity: component2185914
display id: component2185914
version: 1
top level: BBa_K737051
access: public
definition: GFP report

BBa_J23106
type: Component
persistent identity: component2185885
display id: component2185885
version: 1
top level: BBa_K737051
access: public
definition: https://synbiohub.org/public/igem/BBa_J23106/1

BBa_P0412
type: Component
persistent identity: component2185898
display id: component2185898
version: 1
top level: BBa_K737051
access: public
definition: https://synbiohub.org/public/igem/BBa_P0412/1

BBa_E0240
type: SequenceAnnotation
persistent identity: annotation2185914
display id: annotation2185914
version: 1
top level: BBa_K737051
location: https://synbiohub.org/public/igem/BBa_K737051/annotation2185914/range2185914/1
component: BBa_E0240

https://synbiohub.org/public/igem/BBa_K737051/annotation2185914/range2185914/1
type: Range
persistent identity: range2185914
display id: range2185914
version: 1
direction: direction/reverse
top level: BBa_K737051
start: 1423
end: 2298
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation2185899
display id: annotation2185899
version: 1
top level: BBa_K737051
location: https://synbiohub.org/public/igem/BBa_K737051/annotation2185899/range2185899/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_K737051/annotation2185899/range2185899/1
type: Range
persistent identity: range2185899
display id: range2185899
version: 1
direction: direction/forward
top level: BBa_K737051
start: 1360
end: 1413
orientation: inline

BBa_P0412
type: SequenceAnnotation
persistent identity: annotation2185898
display id: annotation2185898
version: 1
top level: BBa_K737051
location: https://synbiohub.org/public/igem/BBa_K737051/annotation2185898/range2185898/1
component: BBa_P0412

https://synbiohub.org/public/igem/BBa_K737051/annotation2185898/range2185898/1
type: Range
persistent identity: range2185898
display id: range2185898
version: 1
direction: direction/reverse
top level: BBa_K737051
start: 44
end: 1351
orientation: inline

BBa_J23106
type: SequenceAnnotation
persistent identity: annotation2185885
display id: annotation2185885
version: 1
top level: BBa_K737051
location: https://synbiohub.org/public/igem/BBa_K737051/annotation2185885/range2185885/1
component: BBa_J23106

https://synbiohub.org/public/igem/BBa_K737051/annotation2185885/range2185885/1
type: Range
persistent identity: range2185885
display id: range2185885
version: 1
direction: direction/reverse
top level: BBa_K737051
start: 1
end: 35
orientation: inline

BBa_A340620
type: ComponentDefinition
persistent identity: BBa A340620
display id: BBa_A340620
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Quadpart Amplifier (B0034.C0062.B0015.R0062)
creator: cconboy
created: 2004-08-09T11:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 60
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Inverter
component: BBa_C0062
component: BBa_R0062
component: BBa_B0034
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_R0062
sequence annotation: BBa_B0034
sequence annotation: BBa_B0012
sequence annotation: BBa_C0062
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_A340620_sequence/1

BBa_C0062
type: Component
persistent identity: component1041202
display id: component1041202
version: 1
top level: BBa_A340620
access: public
definition: luxr

BBa_R0062
type: Component
persistent identity: component1041235
display id: component1041235
version: 1
top level: BBa_A340620
access: public
definition: lux pR

BBa_B0034
type: Component
persistent identity: component1041187
display id: component1041187
version: 1
top level: BBa_A340620
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: Component
persistent identity: component1041220
display id: component1041220
version: 1
top level: BBa_A340620
access: public
definition: BBa_B0012

BBa_B0010
type: Component
persistent identity: component1041210
display id: component1041210
version: 1
top level: BBa_A340620
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation1041235
display id: annotation1041235
version: 1
top level: BBa_A340620
location: https://synbiohub.org/public/igem/BBa_A340620/annotation1041235/range1041235/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_A340620/annotation1041235/range1041235/1
type: Range
persistent identity: range1041235
display id: range1041235
version: 1
direction: direction/forward
top level: BBa_A340620
start: 945
end: 999
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1041187
display id: annotation1041187
version: 1
top level: BBa_A340620
location: https://synbiohub.org/public/igem/BBa_A340620/annotation1041187/range1041187/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_A340620/annotation1041187/range1041187/1
type: Range
persistent identity: range1041187
display id: range1041187
version: 1
direction: direction/forward
top level: BBa_A340620
start: 1
end: 12
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1041220
display id: annotation1041220
version: 1
top level: BBa_A340620
location: https://synbiohub.org/public/igem/BBa_A340620/annotation1041220/range1041220/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_A340620/annotation1041220/range1041220/1
type: Range
persistent identity: range1041220
display id: range1041220
version: 1
direction: direction/forward
top level: BBa_A340620
start: 896
end: 936
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation1041202
display id: annotation1041202
version: 1
top level: BBa_A340620
location: https://synbiohub.org/public/igem/BBa_A340620/annotation1041202/range1041202/1
component: BBa_C0062

https://synbiohub.org/public/igem/BBa_A340620/annotation1041202/range1041202/1
type: Range
persistent identity: range1041202
display id: range1041202
version: 1
direction: direction/forward
top level: BBa_A340620
start: 19
end: 774
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1041210
display id: annotation1041210
version: 1
top level: BBa_A340620
location: https://synbiohub.org/public/igem/BBa_A340620/annotation1041210/range1041210/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_A340620/annotation1041210/range1041210/1
type: Range
persistent identity: range1041210
display id: range1041210
version: 1
direction: direction/forward
top level: BBa_A340620
start: 808
end: 887
orientation: inline

BBa_I732912
type: ComponentDefinition
persistent identity: BBa I732912
display id: BBa_I732912
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Adapter + LacI
creator: Zhan Jian
created: 2007-10-16T11:00:00Z
modified: 2015-08-31T04:08:00Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_I732007
component: BBa_B0034
component: BBa_B0010
component: BBa_I732100
component: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_I732007
sequence annotation: BBa_B0034
sequence annotation: BBa_B0012
sequence annotation: BBa_I732100
sequence: https://synbiohub.org/public/igem/BBa_I732912_sequence/1

BBa_I732007
type: Component
persistent identity: component1948767
display id: component1948767
version: 1
top level: BBa_I732912
access: public
definition: Adapter

BBa_B0034
type: Component
persistent identity: component1948769
display id: component1948769
version: 1
top level: BBa_I732912
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component1948771
display id: component1948771
version: 1
top level: BBa_I732912
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_I732100
type: Component
persistent identity: component1948770
display id: component1948770
version: 1
top level: BBa_I732912
access: public
definition: https://synbiohub.org/public/igem/BBa_I732100/1

BBa_B0012
type: Component
persistent identity: component1948773
display id: component1948773
version: 1
top level: BBa_I732912
access: public
definition: BBa_B0012

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1948771
display id: annotation1948771
version: 1
top level: BBa_I732912
location: https://synbiohub.org/public/igem/BBa_I732912/annotation1948771/range1948771/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I732912/annotation1948771/range1948771/1
type: Range
persistent identity: range1948771
display id: range1948771
version: 1
direction: direction/forward
top level: BBa_I732912
start: 1140
end: 1219
orientation: inline

BBa_I732007
type: SequenceAnnotation
persistent identity: annotation1948767
display id: annotation1948767
version: 1
top level: BBa_I732912
location: https://synbiohub.org/public/igem/BBa_I732912/annotation1948767/range1948767/1
component: BBa_I732007

https://synbiohub.org/public/igem/BBa_I732912/annotation1948767/range1948767/1
type: Range
persistent identity: range1948767
display id: range1948767
version: 1
direction: direction/reverse
top level: BBa_I732912
start: 1
end: 19
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1948769
display id: annotation1948769
version: 1
top level: BBa_I732912
location: https://synbiohub.org/public/igem/BBa_I732912/annotation1948769/range1948769/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I732912/annotation1948769/range1948769/1
type: Range
persistent identity: range1948769
display id: range1948769
version: 1
direction: direction/reverse
top level: BBa_I732912
start: 28
end: 39
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1948773
display id: annotation1948773
version: 1
top level: BBa_I732912
location: https://synbiohub.org/public/igem/BBa_I732912/annotation1948773/range1948773/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I732912/annotation1948773/range1948773/1
type: Range
persistent identity: range1948773
display id: range1948773
version: 1
direction: direction/forward
top level: BBa_I732912
start: 1228
end: 1268
orientation: inline

BBa_I732100
type: SequenceAnnotation
persistent identity: annotation1948770
display id: annotation1948770
version: 1
top level: BBa_I732912
location: https://synbiohub.org/public/igem/BBa_I732912/annotation1948770/range1948770/1
component: BBa_I732100

https://synbiohub.org/public/igem/BBa_I732912/annotation1948770/range1948770/1
type: Range
persistent identity: range1948770
display id: range1948770
version: 1
direction: direction/reverse
top level: BBa_I732912
start: 46
end: 1131
orientation: inline

BBa_I13450
type: ComponentDefinition
persistent identity: BBa I13450
display id: BBa_I13450
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Removes BioBrick End
creator: jkm
created: 2005-05-24T11:00:00Z
modified: 2015-08-31T04:07:34Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Contains both an early MfeI site and a late NsiI site. Cutting with Mfe and Spe and ligation to a BB part cut EX will form two mixed sites, neither of which can be recut. Cutting with Xba and Nsi and ligation to a BB part cut SP will again form two mixed sites, neither of which can be recut.
mutable notes: Should be grown in pSB1A2, as Nsi cuts both pSB2K3 and pSB1AK3 and Mfe cuts pSB4A3. 70 bp from Xba to Nsi or Mfe to Spe, the minimum for gel extraction. The middle is largely noise, to raise the size of the part.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: MfeI
sequence annotation: NsiI
sequence: https://synbiohub.org/public/igem/BBa_I13450_sequence/1

MfeI
type: SequenceAnnotation
persistent identity: annotation1494720
display id: annotation1494720
version: 1
top level: BBa_I13450
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I13450/annotation1494720/range1494720/1

https://synbiohub.org/public/igem/BBa_I13450/annotation1494720/range1494720/1
type: Range
persistent identity: range1494720
display id: range1494720
version: 1
direction: direction/forward
top level: BBa_I13450
start: 2
end: 7
orientation: inline

NsiI
type: SequenceAnnotation
persistent identity: annotation1494630
display id: annotation1494630
version: 1
top level: BBa_I13450
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I13450/annotation1494630/range1494630/1

https://synbiohub.org/public/igem/BBa_I13450/annotation1494630/range1494630/1
type: Range
persistent identity: range1494630
display id: range1494630
version: 1
direction: direction/forward
top level: BBa_I13450
start: 68
end: 73
orientation: inline

BBa_K775000
type: ComponentDefinition
persistent identity: BBa K775000
display id: BBa_K775000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RI7-GPR109A Niacin chimeric GPCR
creator: Sietske Grijseels
created: 2012-08-30T11:00:00Z
modified: 2015-05-08T01:13:15Z
discontinued: false
dominant: false
group u list: _1027_
m user id: 0
owner id: 12035
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: BBa_K775000 encodes a chimeric niacin-sensing G-protein coupled receptor which can be functionally expressed in S. cerevisiae. This chimeric receptor is composed of the ligand-binding domain of the mammalian GPR109A, flanked by the N- and C- terminals of the rat OR RI7.
mutable notes: Promoter: GPD Terminator: CYC1.
mutable provenance: Homo sapiens NP_808219.1 Rat
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: C terminus RI7
sequence annotation: Kozak sequence
sequence annotation: NdeI restriction site
sequence annotation: N terminus RI7
sequence annotation: FLAGtag
sequence annotation: CYC1 terminator
sequence annotation: Niacin receptor part
sequence annotation: Stopcodon
sequence annotation: Startcodon
sequence annotation: GPD promoter
sequence annotation: BamHI restriction site
sequence: https://synbiohub.org/public/igem/BBa_K775000_sequence/1

C terminus RI7
type: SequenceAnnotation
persistent identity: annotation2181647
display id: annotation2181647
version: 1
top level: BBa_K775000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K775000/annotation2181647/range2181647/1

https://synbiohub.org/public/igem/BBa_K775000/annotation2181647/range2181647/1
type: Range
persistent identity: range2181647
display id: range2181647
version: 1
direction: direction/forward
top level: BBa_K775000
start: 1055
end: 1150
orientation: inline

Kozak sequence
type: SequenceAnnotation
persistent identity: annotation2181640
display id: annotation2181640
version: 1
top level: BBa_K775000
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K775000/annotation2181640/range2181640/1

https://synbiohub.org/public/igem/BBa_K775000/annotation2181640/range2181640/1
type: Range
persistent identity: range2181640
display id: range2181640
version: 1
direction: direction/forward
top level: BBa_K775000
start: 119
end: 124
orientation: inline

NdeI restriction site
type: SequenceAnnotation
persistent identity: annotation2181645
display id: annotation2181645
version: 1
top level: BBa_K775000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K775000/annotation2181645/range2181645/1

https://synbiohub.org/public/igem/BBa_K775000/annotation2181645/range2181645/1
type: Range
persistent identity: range2181645
display id: range2181645
version: 1
direction: direction/forward
top level: BBa_K775000
start: 1157
end: 1162
orientation: inline

N terminus RI7
type: SequenceAnnotation
persistent identity: annotation2181643
display id: annotation2181643
version: 1
top level: BBa_K775000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K775000/annotation2181643/range2181643/1

https://synbiohub.org/public/igem/BBa_K775000/annotation2181643/range2181643/1
type: Range
persistent identity: range2181643
display id: range2181643
version: 1
direction: direction/forward
top level: BBa_K775000
start: 152
end: 337
orientation: inline

FLAGtag
type: SequenceAnnotation
persistent identity: annotation2181642
display id: annotation2181642
version: 1
top level: BBa_K775000
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K775000/annotation2181642/range2181642/1

https://synbiohub.org/public/igem/BBa_K775000/annotation2181642/range2181642/1
type: Range
persistent identity: range2181642
display id: range2181642
version: 1
direction: direction/forward
top level: BBa_K775000
start: 128
end: 151
orientation: inline

CYC1 terminator
type: SequenceAnnotation
persistent identity: annotation2181646
display id: annotation2181646
version: 1
top level: BBa_K775000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K775000/annotation2181646/range2181646/1

https://synbiohub.org/public/igem/BBa_K775000/annotation2181646/range2181646/1
type: Range
persistent identity: range2181646
display id: range2181646
version: 1
direction: direction/forward
top level: BBa_K775000
start: 1163
end: 1414
orientation: inline

Niacin receptor part
type: SequenceAnnotation
persistent identity: annotation2181648
display id: annotation2181648
version: 1
top level: BBa_K775000
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K775000/annotation2181648/range2181648/1

https://synbiohub.org/public/igem/BBa_K775000/annotation2181648/range2181648/1
type: Range
persistent identity: range2181648
display id: range2181648
version: 1
direction: direction/forward
top level: BBa_K775000
start: 338
end: 1054
orientation: inline

Stopcodon
type: SequenceAnnotation
persistent identity: annotation2181644
display id: annotation2181644
version: 1
top level: BBa_K775000
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K775000/annotation2181644/range2181644/1

https://synbiohub.org/public/igem/BBa_K775000/annotation2181644/range2181644/1
type: Range
persistent identity: range2181644
display id: range2181644
version: 1
direction: direction/forward
top level: BBa_K775000
start: 1151
end: 1156
orientation: inline

Startcodon
type: SequenceAnnotation
persistent identity: annotation2181641
display id: annotation2181641
version: 1
top level: BBa_K775000
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K775000/annotation2181641/range2181641/1

https://synbiohub.org/public/igem/BBa_K775000/annotation2181641/range2181641/1
type: Range
persistent identity: range2181641
display id: range2181641
version: 1
direction: direction/forward
top level: BBa_K775000
start: 125
end: 127
orientation: inline

GPD promoter
type: SequenceAnnotation
persistent identity: annotation2181638
display id: annotation2181638
version: 1
top level: BBa_K775000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K775000/annotation2181638/range2181638/1

https://synbiohub.org/public/igem/BBa_K775000/annotation2181638/range2181638/1
type: Range
persistent identity: range2181638
display id: range2181638
version: 1
direction: direction/forward
top level: BBa_K775000
start: 1
end: 112
orientation: inline

BamHI restriction site
type: SequenceAnnotation
persistent identity: annotation2181639
display id: annotation2181639
version: 1
top level: BBa_K775000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K775000/annotation2181639/range2181639/1

https://synbiohub.org/public/igem/BBa_K775000/annotation2181639/range2181639/1
type: Range
persistent identity: range2181639
display id: range2181639
version: 1
direction: direction/forward
top level: BBa_K775000
start: 113
end: 118
orientation: inline

BBa_S03156
type: ComponentDefinition
persistent identity: BBa S03156
display id: BBa_S03156
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0034.C0079
creator: Jennifer Braff
created: 2004-07-22T11:00:00Z
modified: 2015-05-08T01:14:20Z
discontinued: false
dominant: true
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 60
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_B0034
component: BBa_C0079
sequence annotation: BBa_C0079
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_S03156_sequence/1

BBa_B0034
type: Component
persistent identity: component940976
display id: component940976
version: 1
top level: BBa_S03156
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_C0079
type: Component
persistent identity: component940989
display id: component940989
version: 1
top level: BBa_S03156
access: public
definition: https://synbiohub.org/public/igem/BBa_C0079/1

BBa_C0079
type: SequenceAnnotation
persistent identity: annotation940989
display id: annotation940989
version: 1
top level: BBa_S03156
location: https://synbiohub.org/public/igem/BBa_S03156/annotation940989/range940989/1
component: BBa_C0079

https://synbiohub.org/public/igem/BBa_S03156/annotation940989/range940989/1
type: Range
persistent identity: range940989
display id: range940989
version: 1
direction: direction/forward
top level: BBa_S03156
start: 19
end: 774
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation940976
display id: annotation940976
version: 1
top level: BBa_S03156
location: https://synbiohub.org/public/igem/BBa_S03156/annotation940976/range940976/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_S03156/annotation940976/range940976/1
type: Range
persistent identity: range940976
display id: range940976
version: 1
direction: direction/forward
top level: BBa_S03156
start: 1
end: 12
orientation: inline

Ara
type: ComponentDefinition
persistent identity: BBa K808000
display id: BBa_K808000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: araC-Pbad - Arabinose inducible regulatory promoter/repressor unit
creator: Valentina Herbring, Sebastian Palluk, Andreas Schmidt
created: 2012-08-10T11:00:00Z
modified: 2015-05-08T01:13:25Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1065_
m user id: 0
owner id: 11792
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This promoter can be induced by arabinose. In presens of arabinose the promotor is active.
mutable notes: non
mutable provenance: Guzman LM, Belin D, Carson MJ, Beckwith J. Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter. J Bacteriol. 1995 Jul;177(14):4121-30 Khlebnikov A, Skaug T, Keasling JD, modulation of gene expression from the arabinose-inducible araBAD promoter,J Ind Microbiol Biotechnol. 2002 Jul;29(1):34-7.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: AraO
sequence annotation: AraO1
sequence annotation: AraPromoter
sequence annotation: AraC
sequence: https://synbiohub.org/public/igem/BBa_K808000_sequence/1

AraO
type: SequenceAnnotation
persistent identity: annotation2200758
display id: annotation2200758
version: 1
top level: Ara
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K808000/annotation2200758/range2200758/1

https://synbiohub.org/public/igem/BBa_K808000/annotation2200758/range2200758/1
type: Range
persistent identity: range2200758
display id: range2200758
version: 1
direction: direction/forward
top level: Ara
start: 909
end: 924
orientation: inline

AraO1
type: SequenceAnnotation
persistent identity: annotation2200759
display id: annotation2200759
version: 1
top level: Ara
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K808000/annotation2200759/range2200759/1

https://synbiohub.org/public/igem/BBa_K808000/annotation2200759/range2200759/1
type: Range
persistent identity: range2200759
display id: range2200759
version: 1
direction: direction/forward
top level: Ara
start: 1066
end: 1077
orientation: inline

AraPromoter
type: SequenceAnnotation
persistent identity: annotation2200760
display id: annotation2200760
version: 1
top level: Ara
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K808000/annotation2200760/range2200760/1

https://synbiohub.org/public/igem/BBa_K808000/annotation2200760/range2200760/1
type: Range
persistent identity: range2200760
display id: range2200760
version: 1
direction: direction/forward
top level: Ara
start: 1153
end: 1181
orientation: inline

AraC
type: SequenceAnnotation
persistent identity: annotation2200757
display id: annotation2200757
version: 1
top level: Ara
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K808000/annotation2200757/range2200757/1

https://synbiohub.org/public/igem/BBa_K808000/annotation2200757/range2200757/1
type: Range
persistent identity: range2200757
display id: range2200757
version: 1
direction: direction/reverse
top level: Ara
start: 1
end: 879
orientation: inline

rbs+COMT
type: ComponentDefinition
persistent identity: BBa I742109
display id: BBa_I742109
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: COMT gene with ribosome binding site
creator: sarah hollingshead
created: 2007-10-18T11:00:00Z
modified: 2015-08-31T04:08:02Z
discontinued: false
dominant: false
experience: experience/None
group u list: _123_
m user id: 0
owner id: 1964
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: COMT (caffeic acid-O-methyl transferase) with ribosome binding site. Enzyme methylates -OH on C4 of the caffeic acid aromatic ring to produce ferulic acid. COMT is the third gene in the vanillin biosynthesis pathway,
mutable notes: Used COMT cDNA as genomic DNA contains introns
mutable provenance: Genes and Enzymes Involved in Caffeic Acid Biosynthesis in the Actinomycete Saccharothrix esanaensis, Berner M, et al, Journal of Bacteriology 188(7): 2666, (2006) Gowri G, et al, Molecular cloning and expression of alfalfa S-adenosyl-L-methionine: caffeic acid 3-0-methyltransferase, a key enzyme of lignin biosynthesis, Plant Physiology 97(1):7 (1991) Downregulation of Caffeic Acid 3-O-Methyltransferase and Caffeoyl CoA 3-O-Methyltransferase in Transgenic Alfalfa Impacts on Lignin Structure and Implications for the Biosynthesis of G and S Lignin, Guo D, et al, Plant Cell. January; 13(1): 73 (2001)
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_J15001
component: BBa_I742107
sequence annotation: BBa_J15001
sequence annotation: BBa_I742107
sequence: https://synbiohub.org/public/igem/BBa_I742109_sequence/1

BBa_J15001
type: Component
persistent identity: component1949770
display id: component1949770
version: 1
top level: rbs+COMT
access: public
definition: https://synbiohub.org/public/igem/BBa_J15001/1

BBa_I742107
type: Component
persistent identity: component1949771
display id: component1949771
version: 1
top level: rbs+COMT
access: public
definition: https://synbiohub.org/public/igem/BBa_I742107/1

BBa_J15001
type: SequenceAnnotation
persistent identity: annotation1949770
display id: annotation1949770
version: 1
top level: rbs+COMT
location: https://synbiohub.org/public/igem/BBa_I742109/annotation1949770/range1949770/1
component: BBa_J15001

https://synbiohub.org/public/igem/BBa_I742109/annotation1949770/range1949770/1
type: Range
persistent identity: range1949770
display id: range1949770
version: 1
direction: direction/reverse
top level: rbs+COMT
start: 1
end: 10
orientation: inline

BBa_I742107
type: SequenceAnnotation
persistent identity: annotation1949771
display id: annotation1949771
version: 1
top level: rbs+COMT
location: https://synbiohub.org/public/igem/BBa_I742109/annotation1949771/range1949771/1
component: BBa_I742107

https://synbiohub.org/public/igem/BBa_I742109/annotation1949771/range1949771/1
type: Range
persistent identity: range1949771
display id: range1949771
version: 1
direction: direction/reverse
top level: rbs+COMT
start: 17
end: 1117
orientation: inline

BBa_I13452
type: ComponentDefinition
persistent identity: BBa I13452
display id: BBa_I13452
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Adds another BioBrick site
creator: jkm
created: 2005-05-24T11:00:00Z
modified: 2015-08-31T04:07:34Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Contains both a BbsI and a BstXI restriction site. When cut with both enzymes, it leaves an EcoRI and a PstI site.
mutable notes: 70 bp from Eco to Spe or Xba to Pst, to allow for gel extraction. The middle portion is largely noise, intended as filler to raise the size of the part.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: BstXI site
sequence annotation: Future Pst site
sequence annotation: Future Eco site
sequence annotation: BbsI site
sequence: https://synbiohub.org/public/igem/BBa_I13452_sequence/1

BstXI site
type: SequenceAnnotation
persistent identity: annotation1891621
display id: annotation1891621
version: 1
top level: BBa_I13452
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I13452/annotation1891621/range1891621/1

https://synbiohub.org/public/igem/BBa_I13452/annotation1891621/range1891621/1
type: Range
persistent identity: range1891621
display id: range1891621
version: 1
direction: direction/forward
top level: BBa_I13452
start: 41
end: 52
orientation: inline

Future Pst site
type: SequenceAnnotation
persistent identity: annotation1891622
display id: annotation1891622
version: 1
top level: BBa_I13452
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I13452/annotation1891622/range1891622/1

https://synbiohub.org/public/igem/BBa_I13452/annotation1891622/range1891622/1
type: Range
persistent identity: range1891622
display id: range1891622
version: 1
direction: direction/forward
top level: BBa_I13452
start: 44
end: 49
orientation: inline

Future Eco site
type: SequenceAnnotation
persistent identity: annotation1891619
display id: annotation1891619
version: 1
top level: BBa_I13452
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I13452/annotation1891619/range1891619/1

https://synbiohub.org/public/igem/BBa_I13452/annotation1891619/range1891619/1
type: Range
persistent identity: range1891619
display id: range1891619
version: 1
direction: direction/forward
top level: BBa_I13452
start: 1
end: 6
orientation: inline

BbsI site
type: SequenceAnnotation
persistent identity: annotation1891620
display id: annotation1891620
version: 1
top level: BBa_I13452
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I13452/annotation1891620/range1891620/1

https://synbiohub.org/public/igem/BBa_I13452/annotation1891620/range1891620/1
type: Range
persistent identity: range1891620
display id: range1891620
version: 1
direction: direction/forward
top level: BBa_I13452
start: 8
end: 13
orientation: inline

BBa_I13519
type: ComponentDefinition
persistent identity: BBa I13519
display id: BBa_I13519
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Screening Plasmid Intermediate
creator: jkm
created: 2005-06-05T11:00:00Z
modified: 2015-08-31T04:07:34Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Built by Josh as an intermediate in screening plasmid construction.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0032
component: BBa_I13452
component: BBa_E0040
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_E0040
sequence annotation: BBa_B0010
sequence annotation: BBa_I13452
sequence annotation: BBa_B0032
sequence: https://synbiohub.org/public/igem/BBa_I13519_sequence/1

BBa_B0032
type: Component
persistent identity: component1505573
display id: component1505573
version: 1
top level: BBa_I13519
access: public
definition: BBa_B0032

BBa_I13452
type: Component
persistent identity: component1505565
display id: component1505565
version: 1
top level: BBa_I13519
access: public
definition: BBa_I13452

BBa_E0040
type: Component
persistent identity: component1505576
display id: component1505576
version: 1
top level: BBa_I13519
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_B0012
type: Component
persistent identity: component1505591
display id: component1505591
version: 1
top level: BBa_I13519
access: public
definition: BBa_B0012

BBa_B0010
type: Component
persistent identity: component1505581
display id: component1505581
version: 1
top level: BBa_I13519
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1505591
display id: annotation1505591
version: 1
top level: BBa_I13519
location: https://synbiohub.org/public/igem/BBa_I13519/annotation1505591/range1505591/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13519/annotation1505591/range1505591/1
type: Range
persistent identity: range1505591
display id: range1505591
version: 1
direction: direction/forward
top level: BBa_I13519
start: 896
end: 936
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1505576
display id: annotation1505576
version: 1
top level: BBa_I13519
location: https://synbiohub.org/public/igem/BBa_I13519/annotation1505576/range1505576/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_I13519/annotation1505576/range1505576/1
type: Range
persistent identity: range1505576
display id: range1505576
version: 1
direction: direction/forward
top level: BBa_I13519
start: 80
end: 799
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1505581
display id: annotation1505581
version: 1
top level: BBa_I13519
location: https://synbiohub.org/public/igem/BBa_I13519/annotation1505581/range1505581/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13519/annotation1505581/range1505581/1
type: Range
persistent identity: range1505581
display id: range1505581
version: 1
direction: direction/forward
top level: BBa_I13519
start: 808
end: 887
orientation: inline

BBa_I13452
type: SequenceAnnotation
persistent identity: annotation1505565
display id: annotation1505565
version: 1
top level: BBa_I13519
location: https://synbiohub.org/public/igem/BBa_I13519/annotation1505565/range1505565/1
component: BBa_I13452

https://synbiohub.org/public/igem/BBa_I13519/annotation1505565/range1505565/1
type: Range
persistent identity: range1505565
display id: range1505565
version: 1
direction: direction/forward
top level: BBa_I13519
start: 1
end: 52
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1505573
display id: annotation1505573
version: 1
top level: BBa_I13519
location: https://synbiohub.org/public/igem/BBa_I13519/annotation1505573/range1505573/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_I13519/annotation1505573/range1505573/1
type: Range
persistent identity: range1505573
display id: range1505573
version: 1
direction: direction/forward
top level: BBa_I13519
start: 61
end: 73
orientation: inline

BBa_K779500
type: ComponentDefinition
persistent identity: BBa K779500
display id: BBa_K779500
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Long reporter top strand with bulge MammoBlock
creator: Kristjan Eerik Kaseniit
created: 2012-09-28T11:00:00Z
modified: 2015-05-08T01:13:18Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1033_
m user id: 0
owner id: 12684
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Reporter top strand with a 4nt bulge. Modifications: 5' RQ quencher, 3' Alexa florophore, all bases modified with 2'O Methyl (ordered as a DNA oligo with Us instead of Ts). IDT DNA oligo order: /5IAbRQ/mCmA mCmCmC mAmCmU mCmCmC mAmCmU mUmCmU mCmCmC mAmCmC mA/3AlexF488N/
mutable notes: Sequence designed to be orthogonal to the HEK293 transcriptome, and to have a high melting temperature. 4-nucleotide bulge added 10-nt from the 3' end as a potential protection mechanism from cleavage by RISC as suggested by Iba et al. (2011). See more details at the MIT iGEM wiki: http://2012.igem.org/Team:MIT
mutable provenance: De novo design.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence annotation: Domain Sb
sequence annotation: Domain Sa part 1
sequence annotation: Bulge
sequence annotation: Domain Sa part 2
sequence: https://synbiohub.org/public/igem/BBa_K779500_sequence/1

Domain Sb
type: SequenceAnnotation
persistent identity: annotation2204232
display id: annotation2204232
version: 1
top level: BBa_K779500
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K779500/annotation2204232/range2204232/1

https://synbiohub.org/public/igem/BBa_K779500/annotation2204232/range2204232/1
type: Range
persistent identity: range2204232
display id: range2204232
version: 1
direction: direction/forward
top level: BBa_K779500
start: 1
end: 24
orientation: inline

Domain Sa part 1
type: SequenceAnnotation
persistent identity: annotation2204233
display id: annotation2204233
version: 1
top level: BBa_K779500
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K779500/annotation2204233/range2204233/1

https://synbiohub.org/public/igem/BBa_K779500/annotation2204233/range2204233/1
type: Range
persistent identity: range2204233
display id: range2204233
version: 1
direction: direction/forward
top level: BBa_K779500
start: 1
end: 10
orientation: inline

Bulge
type: SequenceAnnotation
persistent identity: annotation2204231
display id: annotation2204231
version: 1
top level: BBa_K779500
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K779500/annotation2204231/range2204231/1

https://synbiohub.org/public/igem/BBa_K779500/annotation2204231/range2204231/1
type: Range
persistent identity: range2204231
display id: range2204231
version: 1
direction: direction/forward
top level: BBa_K779500
start: 11
end: 14
orientation: inline

Domain Sa part 2
type: SequenceAnnotation
persistent identity: annotation2204234
display id: annotation2204234
version: 1
top level: BBa_K779500
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K779500/annotation2204234/range2204234/1

https://synbiohub.org/public/igem/BBa_K779500/annotation2204234/range2204234/1
type: Range
persistent identity: range2204234
display id: range2204234
version: 1
direction: direction/forward
top level: BBa_K779500
start: 15
end: 24
orientation: inline

BBa_I0466
type: ComponentDefinition
persistent identity: BBa I0466
display id: BBa_I0466
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RhlR Protein Generator
creator: mit
created: 2004-07-12T11:00:00Z
modified: 2015-08-31T04:07:29Z
discontinued: false
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 101
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: RhlR can sense N-butyryl-HSL in the media and can activate transcription from R0071. It can be used as part of a receiver device.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_B0012
component: BBa_C0071
component: BBa_B0010
component: BBa_B0034
sequence annotation: BBa_B0010
sequence annotation: BBa_C0071
sequence annotation: BBa_B0034
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_I0466_sequence/1

BBa_B0012
type: Component
persistent identity: component943330
display id: component943330
version: 1
top level: BBa_I0466
access: public
definition: BBa_B0012

BBa_C0071
type: Component
persistent identity: component943314
display id: component943314
version: 1
top level: BBa_I0466
access: public
definition: https://synbiohub.org/public/igem/BBa_C0071/1

BBa_B0010
type: Component
persistent identity: component943320
display id: component943320
version: 1
top level: BBa_I0466
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component943304
display id: component943304
version: 1
top level: BBa_I0466
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation943320
display id: annotation943320
version: 1
top level: BBa_I0466
location: https://synbiohub.org/public/igem/BBa_I0466/annotation943320/range943320/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I0466/annotation943320/range943320/1
type: Range
persistent identity: range943320
display id: range943320
version: 1
direction: direction/forward
top level: BBa_I0466
start: 814
end: 893
orientation: inline

BBa_C0071
type: SequenceAnnotation
persistent identity: annotation943314
display id: annotation943314
version: 1
top level: BBa_I0466
location: https://synbiohub.org/public/igem/BBa_I0466/annotation943314/range943314/1
component: BBa_C0071

https://synbiohub.org/public/igem/BBa_I0466/annotation943314/range943314/1
type: Range
persistent identity: range943314
display id: range943314
version: 1
direction: direction/forward
top level: BBa_I0466
start: 19
end: 780
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation943304
display id: annotation943304
version: 1
top level: BBa_I0466
location: https://synbiohub.org/public/igem/BBa_I0466/annotation943304/range943304/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I0466/annotation943304/range943304/1
type: Range
persistent identity: range943304
display id: range943304
version: 1
direction: direction/forward
top level: BBa_I0466
start: 1
end: 12
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation943330
display id: annotation943330
version: 1
top level: BBa_I0466
location: https://synbiohub.org/public/igem/BBa_I0466/annotation943330/range943330/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I0466/annotation943330/range943330/1
type: Range
persistent identity: range943330
display id: range943330
version: 1
direction: direction/forward
top level: BBa_I0466
start: 902
end: 942
orientation: inline

BBa_K876014
type: ComponentDefinition
persistent identity: BBa K876014
display id: BBa_K876014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pLac-LuxI
creator: Timothy Cogan
created: 2012-09-29T11:00:00Z
modified: 2015-05-08T01:13:38Z
discontinued: false
dominant: false
group u list: _1138_
m user id: 0
owner id: 13836
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Constitutive promoter for LuxI which can be repressed by LacI
mutable notes: Biobrick placement
mutable provenance: Generated from biobricks
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_R0010
component: BBa_C0061
sequence annotation: BBa_C0061
sequence annotation: BBa_R0010
sequence: https://synbiohub.org/public/igem/BBa_K876014_sequence/1

BBa_R0010
type: Component
persistent identity: component2245106
display id: component2245106
version: 1
top level: BBa_K876014
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_C0061
type: Component
persistent identity: component2245114
display id: component2245114
version: 1
top level: BBa_K876014
access: public
definition: luxI

BBa_C0061
type: SequenceAnnotation
persistent identity: annotation2245114
display id: annotation2245114
version: 1
top level: BBa_K876014
location: https://synbiohub.org/public/igem/BBa_K876014/annotation2245114/range2245114/1
component: BBa_C0061

https://synbiohub.org/public/igem/BBa_K876014/annotation2245114/range2245114/1
type: Range
persistent identity: range2245114
display id: range2245114
version: 1
direction: direction/forward
top level: BBa_K876014
start: 207
end: 824
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2245106
display id: annotation2245106
version: 1
top level: BBa_K876014
location: https://synbiohub.org/public/igem/BBa_K876014/annotation2245106/range2245106/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K876014/annotation2245106/range2245106/1
type: Range
persistent identity: range2245106
display id: range2245106
version: 1
direction: direction/forward
top level: BBa_K876014
start: 1
end: 200
orientation: inline

BBa_K808012
type: ComponentDefinition
persistent identity: BBa K808012
display id: BBa_K808012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: tphA2: Catalyzes together with tphA3 TPA to DCD
creator: Sascha Hein, Sven Rumpf, Daniel Sachs
created: 2012-09-01T11:00:00Z
modified: 2015-05-08T01:13:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1065_
m user id: 0
owner id: 11792
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K808012_sequence/1

BBa_K861110
type: ComponentDefinition
persistent identity: BBa K861110
display id: BBa_K861110
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: BcsB,regulator of cellulose synthetase
creator: Xian Xia
created: 2012-09-06T11:00:00Z
modified: 2015-06-17T01:21:30Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1121_
m user id: 0
m user id: 4206
owner id: 12357
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: BcsB is involved in biosynthesis of cellulose, which is a significant constituent of the extracellular matrix observed during multicellular morphotype (rdar) growth. The BcsB protein that was indirectly inferred to bind c-di-GMP.
mutable notes: None
mutable provenance: Escherichia coli str. K-12 substr. MG1655
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K861110_sequence/1

BBa_S0116
type: ComponentDefinition
persistent identity: BBa S0116
display id: BBa_S0116
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0031.C0052
creator: Caitlin Conboy and Jennifer Braff
created: 2003-12-04T12:00:00Z
modified: 2015-05-08T01:14:17Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_C0052
component: BBa_B0031
sequence annotation: BBa_B0031
sequence annotation: BBa_C0052
sequence: https://synbiohub.org/public/igem/BBa_S0116_sequence/1

BBa_C0052
type: Component
persistent identity: component939984
display id: component939984
version: 1
top level: BBa_S0116
access: public
definition: cI 434

BBa_B0031
type: Component
persistent identity: component939974
display id: component939974
version: 1
top level: BBa_S0116
access: public
definition: BBa_B0031

BBa_B0031
type: SequenceAnnotation
persistent identity: annotation939974
display id: annotation939974
version: 1
top level: BBa_S0116
location: https://synbiohub.org/public/igem/BBa_S0116/annotation939974/range939974/1
component: BBa_B0031

https://synbiohub.org/public/igem/BBa_S0116/annotation939974/range939974/1
type: Range
persistent identity: range939974
display id: range939974
version: 1
direction: direction/forward
top level: BBa_S0116
start: 1
end: 14
orientation: inline

BBa_C0052
type: SequenceAnnotation
persistent identity: annotation939984
display id: annotation939984
version: 1
top level: BBa_S0116
location: https://synbiohub.org/public/igem/BBa_S0116/annotation939984/range939984/1
component: BBa_C0052

https://synbiohub.org/public/igem/BBa_S0116/annotation939984/range939984/1
type: Range
persistent identity: range939984
display id: range939984
version: 1
direction: direction/forward
top level: BBa_S0116
start: 21
end: 689
orientation: inline

TD0.6
type: ComponentDefinition
persistent identity: BBa K750010
display id: BBa_K750010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TIME DELAY 0.6:LuxI(RBS0.6)->LuxR->LuxPR->GFP
creator: Sifan Wang, Ruosang Qiu, Shuqin Hu,Zhao Ma,Yunxin Long
created: 2012-09-18T11:00:00Z
modified: 2015-05-08T01:13:12Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1001_
m user id: 0
owner id: 14212
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part contains part5 and part10. Arabinose will activate the promoter Pbad, which will cause production of protein LuxI and LuxR. The protein LuxI, LuxR and LuxPR make up the quorum sensing system, which leads to the expression of GFP.
mutable notes: editing...
mutable provenance: We built this part by biobricks from the DNA distribution kit plates 2012.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_K750007
component: BBa_K750002
sequence annotation: BBa_K750002
sequence annotation: BBa_K750007
sequence: https://synbiohub.org/public/igem/BBa_K750010_sequence/1

BBa_K750007
type: Component
persistent identity: component2312702
display id: component2312702
version: 1
top level: TD0.6
access: public
definition: https://synbiohub.org/public/igem/BBa_K750007/1

BBa_K750002
type: Component
persistent identity: component2312665
display id: component2312665
version: 1
top level: TD0.6
access: public
definition: https://synbiohub.org/public/igem/BBa_K750002/1

BBa_K750002
type: SequenceAnnotation
persistent identity: annotation2312665
display id: annotation2312665
version: 1
top level: TD0.6
location: https://synbiohub.org/public/igem/BBa_K750010/annotation2312665/range2312665/1
component: BBa_K750002

https://synbiohub.org/public/igem/BBa_K750010/annotation2312665/range2312665/1
type: Range
persistent identity: range2312665
display id: range2312665
version: 1
direction: direction/reverse
top level: TD0.6
start: 1
end: 939
orientation: inline

BBa_K750007
type: SequenceAnnotation
persistent identity: annotation2312702
display id: annotation2312702
version: 1
top level: TD0.6
location: https://synbiohub.org/public/igem/BBa_K750010/annotation2312702/range2312702/1
component: BBa_K750007

https://synbiohub.org/public/igem/BBa_K750010/annotation2312702/range2312702/1
type: Range
persistent identity: range2312702
display id: range2312702
version: 1
direction: direction/reverse
top level: TD0.6
start: 948
end: 2967
orientation: inline

BBa_J202005
type: ComponentDefinition
persistent identity: BBa J202005
display id: BBa_J202005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PBAD Mutant#6
creator: Jiyeon Park
created: 2012-07-25T11:00:00Z
modified: 2015-08-31T04:08:37Z
discontinued: false
dominant: false
group u list: _910_
m user id: 0
owner id: 11164
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is PBAD (BBa_I13453) mutant. -67 relative to the araC transcriptional start site was changed from T to A.
mutable notes: In order to regulate transcription from PBAD, araC binds three different sites called araO2, araI1, and araI2. In the absence of L-arabinose, araC binds both araO2 and araI1 and bends DNA preventing transcription from PBAD. In the presence of L-arabinose, however, araC has a conformational change because of L-arabinose and binds araI1 and araI2, enabling transcription from PBAD. Like the wild-type (I13453), this part does not have araO2 site which is essential for araC repressor function. Therefore, araC acts as an activator in the presence of L-arabinose.
mutable provenance: This mutant was created by site-directed mutagenesis.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: araI2
sequence annotation: Mutation
sequence annotation: Transcription Start
sequence annotation: araI1
sequence: https://synbiohub.org/public/igem/BBa_J202005_sequence/1

araI2
type: SequenceAnnotation
persistent identity: annotation2178438
display id: annotation2178438
version: 1
top level: BBa_J202005
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J202005/annotation2178438/range2178438/1

https://synbiohub.org/public/igem/BBa_J202005/annotation2178438/range2178438/1
type: Range
persistent identity: range2178438
display id: range2178438
version: 1
direction: direction/forward
top level: BBa_J202005
start: 61
end: 77
orientation: inline

Mutation
type: SequenceAnnotation
persistent identity: annotation2178439
display id: annotation2178439
version: 1
top level: BBa_J202005
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_J202005/annotation2178439/range2178439/1

https://synbiohub.org/public/igem/BBa_J202005/annotation2178439/range2178439/1
type: Range
persistent identity: range2178439
display id: range2178439
version: 1
direction: direction/forward
top level: BBa_J202005
start: 45
end: 45
orientation: inline

Transcription Start
type: SequenceAnnotation
persistent identity: annotation2178436
display id: annotation2178436
version: 1
top level: BBa_J202005
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_J202005/annotation2178436/range2178436/1

https://synbiohub.org/public/igem/BBa_J202005/annotation2178436/range2178436/1
type: Range
persistent identity: range2178436
display id: range2178436
version: 1
direction: direction/forward
top level: BBa_J202005
start: 112
end: 112
orientation: inline

araI1
type: SequenceAnnotation
persistent identity: annotation2178437
display id: annotation2178437
version: 1
top level: BBa_J202005
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J202005/annotation2178437/range2178437/1

https://synbiohub.org/public/igem/BBa_J202005/annotation2178437/range2178437/1
type: Range
persistent identity: range2178437
display id: range2178437
version: 1
direction: direction/forward
top level: BBa_J202005
start: 40
end: 56
orientation: inline

BBa_S03857
type: ComponentDefinition
persistent identity: BBa S03857
display id: BBa_S03857
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: E0840:I718016
creator: Eimad Shotar
created: 2007-10-25T11:00:00Z
modified: 2015-05-08T01:14:31Z
discontinued: false
dominant: true
group u list: _9_
m user id: 0
owner id: 1568
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_E0040
component: BBa_B0010
component: BBa_B0030
component: BBa_B0012
component: BBa_I718016
sequence annotation: BBa_B0010
sequence annotation: BBa_B0030
sequence annotation: BBa_E0040
sequence annotation: BBa_B0012
sequence annotation: BBa_I718016
sequence: https://synbiohub.org/public/igem/BBa_S03857_sequence/1

BBa_E0040
type: Component
persistent identity: component1956452
display id: component1956452
version: 1
top level: BBa_S03857
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_B0010
type: Component
persistent identity: component1956453
display id: component1956453
version: 1
top level: BBa_S03857
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0030
type: Component
persistent identity: component1956449
display id: component1956449
version: 1
top level: BBa_S03857
access: public
definition: BBa_B0030

BBa_B0012
type: Component
persistent identity: component1956455
display id: component1956455
version: 1
top level: BBa_S03857
access: public
definition: BBa_B0012

BBa_I718016
type: Component
persistent identity: component1956459
display id: component1956459
version: 1
top level: BBa_S03857
access: public
definition: https://synbiohub.org/public/igem/BBa_I718016/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1956453
display id: annotation1956453
version: 1
top level: BBa_S03857
location: https://synbiohub.org/public/igem/BBa_S03857/annotation1956453/range1956453/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_S03857/annotation1956453/range1956453/1
type: Range
persistent identity: range1956453
display id: range1956453
version: 1
direction: direction/forward
top level: BBa_S03857
start: 750
end: 829
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1956449
display id: annotation1956449
version: 1
top level: BBa_S03857
location: https://synbiohub.org/public/igem/BBa_S03857/annotation1956449/range1956449/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_S03857/annotation1956449/range1956449/1
type: Range
persistent identity: range1956449
display id: range1956449
version: 1
direction: direction/forward
top level: BBa_S03857
start: 1
end: 15
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1956452
display id: annotation1956452
version: 1
top level: BBa_S03857
location: https://synbiohub.org/public/igem/BBa_S03857/annotation1956452/range1956452/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_S03857/annotation1956452/range1956452/1
type: Range
persistent identity: range1956452
display id: range1956452
version: 1
direction: direction/reverse
top level: BBa_S03857
start: 22
end: 741
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1956455
display id: annotation1956455
version: 1
top level: BBa_S03857
location: https://synbiohub.org/public/igem/BBa_S03857/annotation1956455/range1956455/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_S03857/annotation1956455/range1956455/1
type: Range
persistent identity: range1956455
display id: range1956455
version: 1
direction: direction/forward
top level: BBa_S03857
start: 838
end: 878
orientation: inline

BBa_I718016
type: SequenceAnnotation
persistent identity: annotation1956459
display id: annotation1956459
version: 1
top level: BBa_S03857
location: https://synbiohub.org/public/igem/BBa_S03857/annotation1956459/range1956459/1
component: BBa_I718016

https://synbiohub.org/public/igem/BBa_S03857/annotation1956459/range1956459/1
type: Range
persistent identity: range1956459
display id: range1956459
version: 1
direction: direction/reverse
top level: BBa_S03857
start: 887
end: 920
orientation: inline

BBa_S0108
type: ComponentDefinition
persistent identity: BBa S0108
display id: BBa_S0108
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0033.C0050
creator: Caitlin Conboy and Jennifer Braff
created: 2003-12-04T12:00:00Z
modified: 2015-05-08T01:14:17Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_B0033
component: BBa_C0050
sequence annotation: BBa_B0033
sequence annotation: BBa_C0050
sequence: https://synbiohub.org/public/igem/BBa_S0108_sequence/1

BBa_B0033
type: Component
persistent identity: component939832
display id: component939832
version: 1
top level: BBa_S0108
access: public
definition: BBa_B0033

BBa_C0050
type: Component
persistent identity: component939848
display id: component939848
version: 1
top level: BBa_S0108
access: public
definition: https://synbiohub.org/public/igem/BBa_C0050/1

BBa_B0033
type: SequenceAnnotation
persistent identity: annotation939832
display id: annotation939832
version: 1
top level: BBa_S0108
location: https://synbiohub.org/public/igem/BBa_S0108/annotation939832/range939832/1
component: BBa_B0033

https://synbiohub.org/public/igem/BBa_S0108/annotation939832/range939832/1
type: Range
persistent identity: range939832
display id: range939832
version: 1
direction: direction/forward
top level: BBa_S0108
start: 1
end: 11
orientation: inline

BBa_C0050
type: SequenceAnnotation
persistent identity: annotation939848
display id: annotation939848
version: 1
top level: BBa_S0108
location: https://synbiohub.org/public/igem/BBa_S0108/annotation939848/range939848/1
component: BBa_C0050

https://synbiohub.org/public/igem/BBa_S0108/annotation939848/range939848/1
type: Range
persistent identity: range939848
display id: range939848
version: 1
direction: direction/forward
top level: BBa_S0108
start: 18
end: 761
orientation: inline

BBa_S03154
type: ComponentDefinition
persistent identity: BBa S03154
display id: BBa_S03154
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0034.C0078
creator: Jennifer Braff
created: 2004-07-22T11:00:00Z
modified: 2015-05-08T01:14:20Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 60
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_B0034
component: BBa_C0078
sequence annotation: BBa_C0078
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_S03154_sequence/1

BBa_B0034
type: Component
persistent identity: component940962
display id: component940962
version: 1
top level: BBa_S03154
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_C0078
type: Component
persistent identity: component940972
display id: component940972
version: 1
top level: BBa_S03154
access: public
definition: https://synbiohub.org/public/igem/BBa_C0078/1

BBa_C0078
type: SequenceAnnotation
persistent identity: annotation940972
display id: annotation940972
version: 1
top level: BBa_S03154
location: https://synbiohub.org/public/igem/BBa_S03154/annotation940972/range940972/1
component: BBa_C0078

https://synbiohub.org/public/igem/BBa_S03154/annotation940972/range940972/1
type: Range
persistent identity: range940972
display id: range940972
version: 1
direction: direction/forward
top level: BBa_S03154
start: 19
end: 660
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation940962
display id: annotation940962
version: 1
top level: BBa_S03154
location: https://synbiohub.org/public/igem/BBa_S03154/annotation940962/range940962/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_S03154/annotation940962/range940962/1
type: Range
persistent identity: range940962
display id: range940962
version: 1
direction: direction/forward
top level: BBa_S03154
start: 1
end: 12
orientation: inline

BBa_S05051
type: ComponentDefinition
persistent identity: BBa S05051
display id: BBa_S05051
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0034:K748003
creator: Lei Qiao
created: 2012-09-14T11:00:00Z
modified: 2015-05-08T01:14:49Z
discontinued: false
dominant: false
group u list: _9_
m user id: 0
owner id: 8564
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_K748003
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_K748003
sequence: https://synbiohub.org/public/igem/BBa_S05051_sequence/1

BBa_K748003
type: Component
persistent identity: component2183422
display id: component2183422
version: 1
top level: BBa_S05051
access: public
definition: BBa_K748003

BBa_B0034
type: Component
persistent identity: component2183421
display id: component2183421
version: 1
top level: BBa_S05051
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2183421
display id: annotation2183421
version: 1
top level: BBa_S05051
location: https://synbiohub.org/public/igem/BBa_S05051/annotation2183421/range2183421/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_S05051/annotation2183421/range2183421/1
type: Range
persistent identity: range2183421
display id: range2183421
version: 1
direction: direction/reverse
top level: BBa_S05051
start: 1
end: 12
orientation: inline

BBa_K748003
type: SequenceAnnotation
persistent identity: annotation2183422
display id: annotation2183422
version: 1
top level: BBa_S05051
location: https://synbiohub.org/public/igem/BBa_S05051/annotation2183422/range2183422/1
component: BBa_K748003

https://synbiohub.org/public/igem/BBa_S05051/annotation2183422/range2183422/1
type: Range
persistent identity: range2183422
display id: range2183422
version: 1
direction: direction/reverse
top level: BBa_S05051
start: 19
end: 1404
orientation: inline

BBa_J04450
type: ComponentDefinition
persistent identity: BBa J04450
display id: BBa_J04450
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RFP Coding Device
creator: Tamar Odle
created: 2005-06-08T11:00:00Z
modified: 2015-08-31T04:08:14Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _16_
m user id: 0
owner id: 328
owning group id: 16
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Contains an IPTG inducible promoter an RBS, RFP, and terminator.
mutable provenance: Davidson Synth-Aces
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_R0010
component: BBa_B0010
component: BBa_E1010
component: BBa_B0012
component: BBa_B0034
sequence annotation: BBa_R0010
sequence annotation: BBa_E1010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_J04450_sequence/1

BBa_R0010
type: Component
persistent identity: component1509394
display id: component1509394
version: 1
top level: BBa_J04450
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_B0010
type: Component
persistent identity: component1509417
display id: component1509417
version: 1
top level: BBa_J04450
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_E1010
type: Component
persistent identity: component1509411
display id: component1509411
version: 1
top level: BBa_J04450
access: public
definition: https://synbiohub.org/public/igem/BBa_E1010/1

BBa_B0012
type: Component
persistent identity: component1509427
display id: component1509427
version: 1
top level: BBa_J04450
access: public
definition: BBa_B0012

BBa_B0034
type: Component
persistent identity: component1509404
display id: component1509404
version: 1
top level: BBa_J04450
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1509394
display id: annotation1509394
version: 1
top level: BBa_J04450
location: https://synbiohub.org/public/igem/BBa_J04450/annotation1509394/range1509394/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_J04450/annotation1509394/range1509394/1
type: Range
persistent identity: range1509394
display id: range1509394
version: 1
direction: direction/forward
top level: BBa_J04450
start: 1
end: 200
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation1509411
display id: annotation1509411
version: 1
top level: BBa_J04450
location: https://synbiohub.org/public/igem/BBa_J04450/annotation1509411/range1509411/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_J04450/annotation1509411/range1509411/1
type: Range
persistent identity: range1509411
display id: range1509411
version: 1
direction: direction/forward
top level: BBa_J04450
start: 227
end: 907
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1509417
display id: annotation1509417
version: 1
top level: BBa_J04450
location: https://synbiohub.org/public/igem/BBa_J04450/annotation1509417/range1509417/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J04450/annotation1509417/range1509417/1
type: Range
persistent identity: range1509417
display id: range1509417
version: 1
direction: direction/forward
top level: BBa_J04450
start: 941
end: 1020
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1509404
display id: annotation1509404
version: 1
top level: BBa_J04450
location: https://synbiohub.org/public/igem/BBa_J04450/annotation1509404/range1509404/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J04450/annotation1509404/range1509404/1
type: Range
persistent identity: range1509404
display id: range1509404
version: 1
direction: direction/forward
top level: BBa_J04450
start: 209
end: 220
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1509427
display id: annotation1509427
version: 1
top level: BBa_J04450
location: https://synbiohub.org/public/igem/BBa_J04450/annotation1509427/range1509427/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J04450/annotation1509427/range1509427/1
type: Range
persistent identity: range1509427
display id: range1509427
version: 1
direction: direction/forward
top level: BBa_J04450
start: 1029
end: 1069
orientation: inline

BBa_P0152
type: ComponentDefinition
persistent identity: BBa P0152
display id: BBa_P0152
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PoPS -> cI (434) [S0166]
creator: Randy Rettberg
created: 2004-04-24T11:00:00Z
modified: 2015-05-08T01:14:10Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Protein generator converting TIPS to the protein cI from 434. Used as the input section for Quad Part Inverter Q01520.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0010
component: BBa_C0052
component: BBa_B0031
component: BBa_B0012
sequence annotation: BBa_B0031
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_C0052
sequence: https://synbiohub.org/public/igem/BBa_P0152_sequence/1

BBa_B0010
type: Component
persistent identity: component944552
display id: component944552
version: 1
top level: BBa_P0152
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_C0052
type: Component
persistent identity: component944545
display id: component944545
version: 1
top level: BBa_P0152
access: public
definition: cI 434

BBa_B0031
type: Component
persistent identity: component944535
display id: component944535
version: 1
top level: BBa_P0152
access: public
definition: BBa_B0031

BBa_B0012
type: Component
persistent identity: component944562
display id: component944562
version: 1
top level: BBa_P0152
access: public
definition: BBa_B0012

BBa_B0031
type: SequenceAnnotation
persistent identity: annotation944535
display id: annotation944535
version: 1
top level: BBa_P0152
location: https://synbiohub.org/public/igem/BBa_P0152/annotation944535/range944535/1
component: BBa_B0031

https://synbiohub.org/public/igem/BBa_P0152/annotation944535/range944535/1
type: Range
persistent identity: range944535
display id: range944535
version: 1
direction: direction/forward
top level: BBa_P0152
start: 1
end: 14
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation944562
display id: annotation944562
version: 1
top level: BBa_P0152
location: https://synbiohub.org/public/igem/BBa_P0152/annotation944562/range944562/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_P0152/annotation944562/range944562/1
type: Range
persistent identity: range944562
display id: range944562
version: 1
direction: direction/forward
top level: BBa_P0152
start: 811
end: 851
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation944552
display id: annotation944552
version: 1
top level: BBa_P0152
location: https://synbiohub.org/public/igem/BBa_P0152/annotation944552/range944552/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_P0152/annotation944552/range944552/1
type: Range
persistent identity: range944552
display id: range944552
version: 1
direction: direction/forward
top level: BBa_P0152
start: 723
end: 802
orientation: inline

BBa_C0052
type: SequenceAnnotation
persistent identity: annotation944545
display id: annotation944545
version: 1
top level: BBa_P0152
location: https://synbiohub.org/public/igem/BBa_P0152/annotation944545/range944545/1
component: BBa_C0052

https://synbiohub.org/public/igem/BBa_P0152/annotation944545/range944545/1
type: Range
persistent identity: range944545
display id: range944545
version: 1
direction: direction/forward
top level: BBa_P0152
start: 21
end: 689
orientation: inline

BBa_S0110
type: ComponentDefinition
persistent identity: BBa S0110
display id: BBa_S0110
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0033.C0052
creator: Caitlin Conboy and Jennifer Braff
created: 2003-12-04T12:00:00Z
modified: 2015-05-08T01:14:17Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_C0052
component: BBa_B0033
sequence annotation: BBa_B0033
sequence annotation: BBa_C0052
sequence: https://synbiohub.org/public/igem/BBa_S0110_sequence/1

BBa_C0052
type: Component
persistent identity: component939884
display id: component939884
version: 1
top level: BBa_S0110
access: public
definition: cI 434

BBa_B0033
type: Component
persistent identity: component939873
display id: component939873
version: 1
top level: BBa_S0110
access: public
definition: BBa_B0033

BBa_B0033
type: SequenceAnnotation
persistent identity: annotation939873
display id: annotation939873
version: 1
top level: BBa_S0110
location: https://synbiohub.org/public/igem/BBa_S0110/annotation939873/range939873/1
component: BBa_B0033

https://synbiohub.org/public/igem/BBa_S0110/annotation939873/range939873/1
type: Range
persistent identity: range939873
display id: range939873
version: 1
direction: direction/forward
top level: BBa_S0110
start: 1
end: 11
orientation: inline

BBa_C0052
type: SequenceAnnotation
persistent identity: annotation939884
display id: annotation939884
version: 1
top level: BBa_S0110
location: https://synbiohub.org/public/igem/BBa_S0110/annotation939884/range939884/1
component: BBa_C0052

https://synbiohub.org/public/igem/BBa_S0110/annotation939884/range939884/1
type: Range
persistent identity: range939884
display id: range939884
version: 1
direction: direction/forward
top level: BBa_S0110
start: 18
end: 686
orientation: inline

BBa_K876063
type: ComponentDefinition
persistent identity: BBa K876063
display id: BBa_K876063
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: luxI-RBS-YFP-Term-Term
creator: Zakaraya Ashour
created: 2012-10-01T11:00:00Z
modified: 2015-05-08T01:13:39Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1138_
m user id: 0
owner id: 13835
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: intermediate part
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_E0432
component: BBa_C0061
sequence annotation: BBa_C0061
sequence annotation: BBa_E0432
sequence: https://synbiohub.org/public/igem/BBa_K876063_sequence/1

BBa_E0432
type: Component
persistent identity: component2259786
display id: component2259786
version: 1
top level: BBa_K876063
access: public
definition: https://synbiohub.org/public/igem/BBa_E0432/1

BBa_C0061
type: Component
persistent identity: component2259770
display id: component2259770
version: 1
top level: BBa_K876063
access: public
definition: luxI

BBa_C0061
type: SequenceAnnotation
persistent identity: annotation2259770
display id: annotation2259770
version: 1
top level: BBa_K876063
location: https://synbiohub.org/public/igem/BBa_K876063/annotation2259770/range2259770/1
component: BBa_C0061

https://synbiohub.org/public/igem/BBa_K876063/annotation2259770/range2259770/1
type: Range
persistent identity: range2259770
display id: range2259770
version: 1
direction: direction/forward
top level: BBa_K876063
start: 1
end: 618
orientation: inline

BBa_E0432
type: SequenceAnnotation
persistent identity: annotation2259786
display id: annotation2259786
version: 1
top level: BBa_K876063
location: https://synbiohub.org/public/igem/BBa_K876063/annotation2259786/range2259786/1
component: BBa_E0432

https://synbiohub.org/public/igem/BBa_K876063/annotation2259786/range2259786/1
type: Range
persistent identity: range2259786
display id: range2259786
version: 1
direction: direction/reverse
top level: BBa_K876063
start: 652
end: 1568
orientation: inline

BBa_I13202
type: ComponentDefinition
persistent identity: BBa I13202
display id: BBa_I13202
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 3OC6HSL Sender Controlled by Lac Repressible Promoter
creator: Barry Canton
created: 2004-07-29T11:00:00Z
modified: 2015-08-31T04:07:33Z
discontinued: false
dominant: true
experience: experience/None
group u list: _101_6_
m user id: 0
owner id: 135
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: A lac repressible promoter driving production of an untagged LuxI enzyme which produces an AHL (3OC6HSL).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_B0012
component: BBa_B0034
component: BBa_B0010
component: BBa_C0161
component: BBa_R0011
sequence annotation: BBa_R0011
sequence annotation: BBa_B0034
sequence annotation: BBa_B0010
sequence annotation: BBa_C0161
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_I13202_sequence/1

BBa_B0012
type: Component
persistent identity: component1484952
display id: component1484952
version: 1
top level: BBa_I13202
access: public
definition: BBa_B0012

BBa_B0034
type: Component
persistent identity: component1484935
display id: component1484935
version: 1
top level: BBa_I13202
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component1484942
display id: component1484942
version: 1
top level: BBa_I13202
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_C0161
type: Component
persistent identity: component1484937
display id: component1484937
version: 1
top level: BBa_I13202
access: public
definition: https://synbiohub.org/public/igem/BBa_C0161/1

BBa_R0011
type: Component
persistent identity: component1484926
display id: component1484926
version: 1
top level: BBa_I13202
access: public
definition: lacI+pL

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation1484926
display id: annotation1484926
version: 1
top level: BBa_I13202
location: https://synbiohub.org/public/igem/BBa_I13202/annotation1484926/range1484926/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_I13202/annotation1484926/range1484926/1
type: Range
persistent identity: range1484926
display id: range1484926
version: 1
direction: direction/forward
top level: BBa_I13202
start: 1
end: 54
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1484935
display id: annotation1484935
version: 1
top level: BBa_I13202
location: https://synbiohub.org/public/igem/BBa_I13202/annotation1484935/range1484935/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13202/annotation1484935/range1484935/1
type: Range
persistent identity: range1484935
display id: range1484935
version: 1
direction: direction/forward
top level: BBa_I13202
start: 64
end: 75
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1484942
display id: annotation1484942
version: 1
top level: BBa_I13202
location: https://synbiohub.org/public/igem/BBa_I13202/annotation1484942/range1484942/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13202/annotation1484942/range1484942/1
type: Range
persistent identity: range1484942
display id: range1484942
version: 1
direction: direction/forward
top level: BBa_I13202
start: 675
end: 754
orientation: inline

BBa_C0161
type: SequenceAnnotation
persistent identity: annotation1484937
display id: annotation1484937
version: 1
top level: BBa_I13202
location: https://synbiohub.org/public/igem/BBa_I13202/annotation1484937/range1484937/1
component: BBa_C0161

https://synbiohub.org/public/igem/BBa_I13202/annotation1484937/range1484937/1
type: Range
persistent identity: range1484937
display id: range1484937
version: 1
direction: direction/forward
top level: BBa_I13202
start: 82
end: 666
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1484952
display id: annotation1484952
version: 1
top level: BBa_I13202
location: https://synbiohub.org/public/igem/BBa_I13202/annotation1484952/range1484952/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13202/annotation1484952/range1484952/1
type: Range
persistent identity: range1484952
display id: range1484952
version: 1
direction: direction/forward
top level: BBa_I13202
start: 763
end: 803
orientation: inline

Adapter
type: ComponentDefinition
persistent identity: BBa I732007
display id: BBa_I732007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: BglII + BamHI
creator: Zhan Jian
created: 2007-07-06T11:00:00Z
modified: 2015-08-31T04:07:56Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: BglII and BamHI restriction sites with some flanking
mutable notes: None
mutable provenance: http://openwetware.org/wiki/Silver:_Oligonucleotide_Inserts Oligonucleotide Inserts
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: BglII
sequence annotation: BamHI
sequence: https://synbiohub.org/public/igem/BBa_I732007_sequence/1

BglII
type: SequenceAnnotation
persistent identity: annotation1936907
display id: annotation1936907
version: 1
top level: Adapter
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I732007/annotation1936907/range1936907/1

https://synbiohub.org/public/igem/BBa_I732007/annotation1936907/range1936907/1
type: Range
persistent identity: range1936907
display id: range1936907
version: 1
direction: direction/forward
top level: Adapter
start: 3
end: 8
orientation: inline

BamHI
type: SequenceAnnotation
persistent identity: annotation1936908
display id: annotation1936908
version: 1
top level: Adapter
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I732007/annotation1936908/range1936908/1

https://synbiohub.org/public/igem/BBa_I732007/annotation1936908/range1936908/1
type: Range
persistent identity: range1936908
display id: range1936908
version: 1
direction: direction/forward
top level: Adapter
start: 12
end: 17
orientation: inline

BBa_I732902
type: ComponentDefinition
persistent identity: BBa I732902
display id: BBa_I732902
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: R0010 I732020
creator: Zhan Jian
created: 2007-10-16T11:00:00Z
modified: 2015-08-31T04:08:00Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_B0034
component: BBa_R0010
component: BBa_I732006
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_I732006
sequence annotation: BBa_R0010
sequence annotation: BBa_B0034
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_I732902_sequence/1

BBa_B0034
type: Component
persistent identity: component1948539
display id: component1948539
version: 1
top level: BBa_I732902
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0010
type: Component
persistent identity: component1948531
display id: component1948531
version: 1
top level: BBa_I732902
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_I732006
type: Component
persistent identity: component1948543
display id: component1948543
version: 1
top level: BBa_I732902
access: public
definition: https://synbiohub.org/public/igem/BBa_I732006/1

BBa_B0012
type: Component
persistent identity: component1948546
display id: component1948546
version: 1
top level: BBa_I732902
access: public
definition: BBa_B0012

BBa_B0010
type: Component
persistent identity: component1948544
display id: component1948544
version: 1
top level: BBa_I732902
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1948544
display id: annotation1948544
version: 1
top level: BBa_I732902
location: https://synbiohub.org/public/igem/BBa_I732902/annotation1948544/range1948544/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I732902/annotation1948544/range1948544/1
type: Range
persistent identity: range1948544
display id: range1948544
version: 1
direction: direction/forward
top level: BBa_I732902
start: 469
end: 548
orientation: inline

BBa_I732006
type: SequenceAnnotation
persistent identity: annotation1948543
display id: annotation1948543
version: 1
top level: BBa_I732902
location: https://synbiohub.org/public/igem/BBa_I732902/annotation1948543/range1948543/1
component: BBa_I732006

https://synbiohub.org/public/igem/BBa_I732902/annotation1948543/range1948543/1
type: Range
persistent identity: range1948543
display id: range1948543
version: 1
direction: direction/reverse
top level: BBa_I732902
start: 227
end: 460
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1948531
display id: annotation1948531
version: 1
top level: BBa_I732902
location: https://synbiohub.org/public/igem/BBa_I732902/annotation1948531/range1948531/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_I732902/annotation1948531/range1948531/1
type: Range
persistent identity: range1948531
display id: range1948531
version: 1
direction: direction/forward
top level: BBa_I732902
start: 1
end: 200
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1948539
display id: annotation1948539
version: 1
top level: BBa_I732902
location: https://synbiohub.org/public/igem/BBa_I732902/annotation1948539/range1948539/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I732902/annotation1948539/range1948539/1
type: Range
persistent identity: range1948539
display id: range1948539
version: 1
direction: direction/reverse
top level: BBa_I732902
start: 209
end: 220
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1948546
display id: annotation1948546
version: 1
top level: BBa_I732902
location: https://synbiohub.org/public/igem/BBa_I732902/annotation1948546/range1948546/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I732902/annotation1948546/range1948546/1
type: Range
persistent identity: range1948546
display id: range1948546
version: 1
direction: direction/forward
top level: BBa_I732902
start: 557
end: 597
orientation: inline

YFP
type: ComponentDefinition
persistent identity: BBa E0032
display id: BBa_E0032
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: enhanced yellow fluorescent protein derived from A. victoria GFP
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:25Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Yellow fluorescent protein (EYFP) reporter coding sequence without the Ribosome Binding Site. Modified with an LVA tail for rapid degradation of the protein and faster fall time for the emission. Annotated mutation cause a Q81L mutation that appears to not be near the active site.
mutable notes: BBa_E0032 yellow fluorescent protein is based on BioBrick part BBa_E0031. It has been modified to include a rapid degradation LVA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.
mutable provenance: Modified from <bb_part>BBa_E0031</bb_part>
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: YFP (LVA)
sequence annotation: BBa_E0032
sequence annotation: 2
sequence annotation: A (Q->L)
sequence annotation: SsrA
sequence: https://synbiohub.org/public/igem/BBa_E0032_sequence/1

YFP (LVA)
type: SequenceAnnotation
persistent identity: annotation2156
display id: annotation2156
version: 1
top level: YFP
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_E0032/annotation2156/range2156/1

https://synbiohub.org/public/igem/BBa_E0032/annotation2156/range2156/1
type: Range
persistent identity: range2156
display id: range2156
version: 1
direction: direction/forward
top level: YFP
start: 1
end: 762
orientation: inline

BBa_E0032
type: SequenceAnnotation
persistent identity: annotation7041
display id: annotation7041
version: 1
top level: YFP
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_E0032/annotation7041/range7041/1

https://synbiohub.org/public/igem/BBa_E0032/annotation7041/range7041/1
type: Range
persistent identity: range7041
display id: range7041
version: 1
direction: direction/forward
top level: YFP
start: 1
end: 762
orientation: inline

2
type: SequenceAnnotation
persistent identity: annotation2159
display id: annotation2159
version: 1
top level: YFP
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_E0032/annotation2159/range2159/1

https://synbiohub.org/public/igem/BBa_E0032/annotation2159/range2159/1
type: Range
persistent identity: range2159
display id: range2159
version: 1
direction: direction/forward
top level: YFP
start: 757
end: 762
orientation: inline

A (Q->L)
type: SequenceAnnotation
persistent identity: annotation2161
display id: annotation2161
version: 1
top level: YFP
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_E0032/annotation2161/range2161/1

https://synbiohub.org/public/igem/BBa_E0032/annotation2161/range2161/1
type: Range
persistent identity: range2161
display id: range2161
version: 1
direction: direction/forward
top level: YFP
start: 242
end: 242
orientation: inline

SsrA
type: SequenceAnnotation
persistent identity: annotation2160
display id: annotation2160
version: 1
top level: YFP
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_E0032/annotation2160/range2160/1

https://synbiohub.org/public/igem/BBa_E0032/annotation2160/range2160/1
type: Range
persistent identity: range2160
display id: range2160
version: 1
direction: direction/forward
top level: YFP
start: 718
end: 756
orientation: inline

BBa_I7108
type: ComponentDefinition
persistent identity: BBa I7108
display id: BBa_I7108
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: R0053.E0840
creator: Jennifer Braff
created: 2005-07-27T11:00:00Z
modified: 2015-08-31T04:07:45Z
discontinued: false
dominant: false
group u list: _11_
m user id: 0
owner id: 61
owning group id: 1
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: R0053.E0840 (S_N; strong rbs; low PoPS)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_B0012
component: BBa_E0040
component: BBa_B0030
component: BBa_B0010
component: BBa_R0053
sequence annotation: BBa_R0053
sequence annotation: BBa_B0010
sequence annotation: BBa_B0030
sequence annotation: BBa_B0012
sequence annotation: BBa_E0040
sequence: https://synbiohub.org/public/igem/BBa_I7108_sequence/1

BBa_B0012
type: Component
persistent identity: component1596691
display id: component1596691
version: 1
top level: BBa_I7108
access: public
definition: BBa_B0012

BBa_E0040
type: Component
persistent identity: component1596676
display id: component1596676
version: 1
top level: BBa_I7108
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_B0030
type: Component
persistent identity: component1596673
display id: component1596673
version: 1
top level: BBa_I7108
access: public
definition: BBa_B0030

BBa_B0010
type: Component
persistent identity: component1596681
display id: component1596681
version: 1
top level: BBa_I7108
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_R0053
type: Component
persistent identity: component1596660
display id: component1596660
version: 1
top level: BBa_I7108
access: public
definition: https://synbiohub.org/public/igem/BBa_R0053/1

BBa_R0053
type: SequenceAnnotation
persistent identity: annotation1596660
display id: annotation1596660
version: 1
top level: BBa_I7108
location: https://synbiohub.org/public/igem/BBa_I7108/annotation1596660/range1596660/1
component: BBa_R0053

https://synbiohub.org/public/igem/BBa_I7108/annotation1596660/range1596660/1
type: Range
persistent identity: range1596660
display id: range1596660
version: 1
direction: direction/forward
top level: BBa_I7108
start: 1
end: 54
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1596681
display id: annotation1596681
version: 1
top level: BBa_I7108
location: https://synbiohub.org/public/igem/BBa_I7108/annotation1596681/range1596681/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I7108/annotation1596681/range1596681/1
type: Range
persistent identity: range1596681
display id: range1596681
version: 1
direction: direction/forward
top level: BBa_I7108
start: 812
end: 891
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1596673
display id: annotation1596673
version: 1
top level: BBa_I7108
location: https://synbiohub.org/public/igem/BBa_I7108/annotation1596673/range1596673/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_I7108/annotation1596673/range1596673/1
type: Range
persistent identity: range1596673
display id: range1596673
version: 1
direction: direction/forward
top level: BBa_I7108
start: 63
end: 77
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1596691
display id: annotation1596691
version: 1
top level: BBa_I7108
location: https://synbiohub.org/public/igem/BBa_I7108/annotation1596691/range1596691/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I7108/annotation1596691/range1596691/1
type: Range
persistent identity: range1596691
display id: range1596691
version: 1
direction: direction/forward
top level: BBa_I7108
start: 900
end: 940
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1596676
display id: annotation1596676
version: 1
top level: BBa_I7108
location: https://synbiohub.org/public/igem/BBa_I7108/annotation1596676/range1596676/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_I7108/annotation1596676/range1596676/1
type: Range
persistent identity: range1596676
display id: range1596676
version: 1
direction: direction/forward
top level: BBa_I7108
start: 84
end: 803
orientation: inline

BBa_K849004
type: ComponentDefinition
persistent identity: BBa K849004
display id: BBa_K849004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ent-Kaurene Oxidase from Gibberella fujikuroi
creator: Martin Schneider
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:34Z
discontinued: false
dominant: false
group u list: _1109_
m user id: 0
owner id: 12313
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: KO
mutable notes: no
mutable provenance: Genomic Sequence from Gibberella fujikuroi
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: cds
sequence: https://synbiohub.org/public/igem/BBa_K849004_sequence/1

cds
type: SequenceAnnotation
persistent identity: annotation2202568
display id: annotation2202568
version: 1
top level: BBa_K849004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K849004/annotation2202568/range2202568/1

https://synbiohub.org/public/igem/BBa_K849004/annotation2202568/range2202568/1
type: Range
persistent identity: range2202568
display id: range2202568
version: 1
direction: direction/forward
top level: BBa_K849004
start: 1
end: 1578
orientation: inline

ecfp
type: ComponentDefinition
persistent identity: BBa I15017
display id: BBa_I15017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0032.EYFP
creator: Jeff Tabor
created: 2005-04-14T11:00:00Z
modified: 2015-08-31T04:07:38Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _5_
m user id: 0
owner id: 88
owning group id: 5
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part accepts POPS to generate EYFP under the medium strength RBS B0032.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_E0030
component: BBa_B0032
sequence annotation: BBa_E0030
sequence annotation: BBa_B0032
sequence: https://synbiohub.org/public/igem/BBa_I15017_sequence/1

BBa_E0030
type: Component
persistent identity: component1478422
display id: component1478422
version: 1
top level: ecfp
access: public
definition: https://synbiohub.org/public/igem/BBa_E0030/1

BBa_B0032
type: Component
persistent identity: component1478419
display id: component1478419
version: 1
top level: ecfp
access: public
definition: BBa_B0032

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation1478422
display id: annotation1478422
version: 1
top level: ecfp
location: https://synbiohub.org/public/igem/BBa_I15017/annotation1478422/range1478422/1
component: BBa_E0030

https://synbiohub.org/public/igem/BBa_I15017/annotation1478422/range1478422/1
type: Range
persistent identity: range1478422
display id: range1478422
version: 1
direction: direction/forward
top level: ecfp
start: 20
end: 742
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1478419
display id: annotation1478419
version: 1
top level: ecfp
location: https://synbiohub.org/public/igem/BBa_I15017/annotation1478419/range1478419/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_I15017/annotation1478419/range1478419/1
type: Range
persistent identity: range1478419
display id: range1478419
version: 1
direction: direction/forward
top level: ecfp
start: 1
end: 13
orientation: inline

BBa_J01142
type: ComponentDefinition
persistent identity: BBa J01142
display id: BBa_J01142
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [pLacI pL][key3]
creator: Golden Bear (Berkeley iGEM 2005)
created: 2006-04-13T11:00:00Z
modified: 2015-08-31T04:08:13Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _13_1_
m user id: 0
owner id: 612
owning group id: 13
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000834
role: partType/RNA
component: BBa_J01086
component: BBa_R0011
sequence annotation: BBa_J01086
sequence annotation: BBa_R0011
sequence: https://synbiohub.org/public/igem/BBa_J01142_sequence/1

BBa_J01086
type: Component
persistent identity: component1843674
display id: component1843674
version: 1
top level: BBa_J01142
access: public
definition: https://synbiohub.org/public/igem/BBa_J01086/1

BBa_R0011
type: Component
persistent identity: component1843668
display id: component1843668
version: 1
top level: BBa_J01142
access: public
definition: lacI+pL

BBa_J01086
type: SequenceAnnotation
persistent identity: annotation1843674
display id: annotation1843674
version: 1
top level: BBa_J01142
location: https://synbiohub.org/public/igem/BBa_J01142/annotation1843674/range1843674/1
component: BBa_J01086

https://synbiohub.org/public/igem/BBa_J01142/annotation1843674/range1843674/1
type: Range
persistent identity: range1843674
display id: range1843674
version: 1
direction: direction/reverse
top level: BBa_J01142
start: 64
end: 157
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation1843668
display id: annotation1843668
version: 1
top level: BBa_J01142
location: https://synbiohub.org/public/igem/BBa_J01142/annotation1843668/range1843668/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_J01142/annotation1843668/range1843668/1
type: Range
persistent identity: range1843668
display id: range1843668
version: 1
direction: direction/forward
top level: BBa_J01142
start: 1
end: 54
orientation: inline

trkD
type: ComponentDefinition
persistent identity: BBa K629005
display id: BBa_K629005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: trkD, a functional Kup (formerly TrkD) system took up Cs+ with a moderate rate and affinity
creator: Zilong WANG, Yi ZHENG
created: 2011-10-03T11:00:00Z
modified: 2015-05-08T01:12:56Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _801_
m user id: 0
owner id: 9022
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Escherichia coli cells which contain a functional Kup (formerly TrkD) system took up Cs' with a moderate rate and affinity. Kup is a separate K+ uptake system with relatively little discrimination in the transport of the cations K+, Rb+, and Cs'. Regardless of the presence or absence of Kup, K+-replete cells took up Cs'primarily by a very low affinity mode, proportional to the ratio of the Cs' and K+ concentrations in the medium. In our project, trkD is used to collect Cs-137 when recN is activated by radiation and start the transcription of trkD,in order to absorb this kind of radioactive source like Cs-137
mutable notes: none
mutable provenance: ATGAGCACTGATAATAAGCAATCATTGCCCGCGATTACCCTCGCGGCGATTGGAGTTGTCTACGGCGATATTGGTACCAGCCCGTTATATACACTTCGTGAATGTTTGTCCGGCCAGTTTGGTTTTGGCGTTGAACGCGATGCCGTGTTTGGCTTTTTATCGCTGATCTTCTGGCTGCTAATCTTTGTGGTTTCCATTAAATATCTCACCTTCGTGATGCGGGCAGATAACGCCGGTGAGGGGGGGATCCTGACGTTGATGTCGCTTGCCGGGCGTAATACGTCGGCGCGAACCACATCAATGCTGGTGATTATGGGGCTAATCGGCGGCAGCTTTTTCTATGGTGAAGTCGTCATAACACCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATTTATGATTCAAAAGCATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCGATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGCTGTCGATTACGGGGGTCGAGGCGCTGTATGCTGATATGGGACACTTTGGTAAGTTCCCTATTCGTCTGGCGTGGTTCACTGTCGTATTACCTTCCTTAACTCTTAATTACTTCGGCCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTTTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATCGCCTCGCAGGCGGTTATCTCTGGCGTCTTTTCATTGACGCGTCAGGCGGTACGTCTGGGATATTTGTCGCCGATGCGCATTATTCACACCTCCGAAATGGAGTCAGGGCAAATCTATATTCCGTTTGTGAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCAGCTTTGAGCACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGGAATAAGTATTTTGTTGCGCTGATCCTGATTGCTTTCCTTTGTGTCGATATTCCATTGTTCACCGCTAACCTCGATAAACTGCTCTCCGGCGGCTGGTTGCCATTGAGCCTCGGTACTGTGATGTTTATCGTGATGACCACCTGGAAGAGCGAGCGTTTCCGCTTGCTGCGGCGGATGCATGAACATGGTAACTCTCTGGAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGCCCGGGACCGCGGTGTATATGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCGGGTGATTCTGTTAACTCTGCGCACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: terminator
sequence annotation: rev primer
sequence annotation: start
sequence annotation: fwd primer
sequence annotation: TrkD
sequence: https://synbiohub.org/public/igem/BBa_K629005_sequence/1

terminator
type: SequenceAnnotation
persistent identity: annotation2157758
display id: annotation2157758
version: 1
top level: trkD
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K629005/annotation2157758/range2157758/1

https://synbiohub.org/public/igem/BBa_K629005/annotation2157758/range2157758/1
type: Range
persistent identity: range2157758
display id: range2157758
version: 1
direction: direction/forward
top level: trkD
start: 1867
end: 1869
orientation: inline

rev primer
type: SequenceAnnotation
persistent identity: annotation2157757
display id: annotation2157757
version: 1
top level: trkD
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K629005/annotation2157757/range2157757/1

https://synbiohub.org/public/igem/BBa_K629005/annotation2157757/range2157757/1
type: Range
persistent identity: range2157757
display id: range2157757
version: 1
direction: direction/reverse
top level: trkD
start: 1849
end: 1869
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2157754
display id: annotation2157754
version: 1
top level: trkD
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K629005/annotation2157754/range2157754/1

https://synbiohub.org/public/igem/BBa_K629005/annotation2157754/range2157754/1
type: Range
persistent identity: range2157754
display id: range2157754
version: 1
direction: direction/forward
top level: trkD
start: 1
end: 3
orientation: inline

fwd primer
type: SequenceAnnotation
persistent identity: annotation2157755
display id: annotation2157755
version: 1
top level: trkD
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K629005/annotation2157755/range2157755/1

https://synbiohub.org/public/igem/BBa_K629005/annotation2157755/range2157755/1
type: Range
persistent identity: range2157755
display id: range2157755
version: 1
direction: direction/forward
top level: trkD
start: 1
end: 21
orientation: inline

TrkD
type: SequenceAnnotation
persistent identity: annotation2157756
display id: annotation2157756
version: 1
top level: trkD
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K629005/annotation2157756/range2157756/1

https://synbiohub.org/public/igem/BBa_K629005/annotation2157756/range2157756/1
type: Range
persistent identity: range2157756
display id: range2157756
version: 1
direction: direction/forward
top level: trkD
start: 1
end: 1869
orientation: inline