This is a MoClo converted version of BBa_J23110
This is a MoClo converted version of BBa_J23110. It has two MoClo fusion sites flanking this part.
We had to design the proper MoClo fusion sites to flank this part.
The MoClo format of BBa_R0040 with AB fusion sites.
===Design Notes=== This is the MoClo formatted part of BBa_R0040 with AB fusion sites. See the BBa_R0040 page for full information on the part. This is a Level 0 MoClo part with flanking sites A on the 5' side and site B on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. Summary of modifications from original part: Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See BBa_K783055 . ===Source=== iGEM Distribution Kit ===References===
The MoClo format of BBa_J23102 with AB fusion sites.
This is the MoClo formatted part of BBa_J23102 with EB fusion sites. See the BBa_J23102 page for full information on the part. Summary of modifications from original part: Backbone has one added SpeI site in front of gene, BsaI sites, and 4bp fusion sites.
The MoClo format of BBa_J23112 with AB fusion sites.
__NOTOC__ <partinfo>BBa_K1114014 short</partinfo> <partinfo>BBa_K1114014 SequenceAndFeatures</partinfo> ===Design Notes=== This is the MoClo formatted part of BBa_J23112 with AB fusion sites. See the BBa_J23112 page for full information on the part. This is a Level 0 MoClo part with flanking sites A on the 5' side and site B on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. Summary of modifications from original part: Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See BBa_K783055 . ===Source=== iGEM Distribution Kit ===References===
The MoClo format of BBa_J23113 with AB fusion sites.
===Design Notes=== This is the MoClo formatted part of BBa_J23113 with AB fusion sites. See the BBa_J23113 page for full information on the part. This is a Level 0 MoClo part with flanking sites A on the 5' side and site B on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. Summary of modifications from original part: Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See BBa_K783055 . ===Source=== iGEM Distribution Kit ===References===
The MoClo format of BBa_J23106 with AB fusion sites.
This is the MoClo formatted part of BBa_J23106 with AB fusion sites. See the BBa_J23106 page for full information on the part. Summary of modifications from original part: Backbone has one added SpeI site in front of gene, BsaI sites, and 4bp fusion sites. The fusion site letters refer to 4bp fusion sites: A=GGAG; B=TACT; C=AATG; D=AGGT; E=GCTT; F = CGCT; G=TGCC; H=ACTA.
Bicistronic Design RBS 2, MoClo Format with BC fusion sites
This is the MoClo formatted part of a bicistronic design ribosomal binding site 1 with BC fusion sites. This is a Level 0 MoClo part with flanking sites B on the 5' side and site C on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See BBa_K783055.
This part was cloned off a BCD template from Sonya Iverson. Sonya Iverson based her designs for her BCD2 part off of a paper published by Mutalik, et al.
This tandem promoter was created using the MoClo assembly method. This is a Level 0 MoClo part with flanking sites A on the 5' side and site B on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. The promoter is induced by atc and arabinose. The characterization data will be updated before the jamboree.
We looked through the literature and decided to attach the two promoters without any extra sequence between them
This was formed using BBa_I13453 and BBa_R0040
This is a MoClo converted version of BBa_R0063
This is the MoClo converted version of BBa_R0063, which is the negatively regulated pLuxR promoter. It has two MoClo fusion sites flanking it.
Bicistronic design RBS 16, MoClo Format with BC fusion sites
This is the MoClo formatted part of a bicistronic design ribosomal binding site 16 with BC fusion sites. This is a Level 0 MoClo part with flanking sites B on the 5' side and site C on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See BBa_K783055.
Bicistronic design RBS 8, MoClo Format with BC fusion sites
This is the MoClo formatted part of a bicistronic design ribosomal binding site 8 with BC fusion sites. This is a Level 0 MoClo part with flanking sites B on the 5' side and site C on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See BBa_K783055.
The MoClo format of BBa_J23100 with AB fusion sites.
This is the MoClo formatted part of BBa_J23100 with AB fusion sites. See the BBa_J23100 page for full information on the part.
This is the MoClo formatted part of BBa_J23100 with AB fusion sites. See the BBa_J23100 page for full information on the part. Summary of Modifications from original part: Backbone has one added SpeI site in front of gene, BsaI sites, and 4bp fusion sites.
The MoClo format of BBa_J23116 with AB fusion sites.
===Design Notes=== This is the MoClo formatted part of BBa_J23116 with AB fusion sites. See the BBa_J23116 page for full information on the part. This is a Level 0 MoClo part with flanking sites A on the 5' side and site B on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. Summary of modifications from original part: Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See BBa_K783055 . ===Source=== iGEM Distribution Kit ===References===
This is a MoClo converted version of BBa_J23115
This is a MoClo converted version of BBa_J23115. It has two MoClo fusion sites flanking it.
MoClo formatted version of B0033 with BC fusion sites
This is the MoClo formatted part of BBa_B0033 with BC fusion sites. See the BBa_B0033 page for full information on the part. This is a Level 0 MoClo part with flanking sites B on the 5' side and site C on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. Summary of modifications from original part: Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See BBa_K783055.
The MoClo format of BBa_J23104 with AB fusion sites.
This is the MoClo formatted part of BBa_R0079 with EB fusion sites. See the BBa_R0079 page for full information on the part. Summary of modifications from original part: Backbone has one added SpeI site in front of gene, BsaI sites, and 4bp fusion sites.
MoClo format of a modified version of BBa_B0031 with BC fusion sites.
This is the MoClo formatted part of a modified BBa_B0031 with BC fusion sites. See the BBa_B0031 page for full information on the part. This is a Level 0 MoClo part with flanking sites B on the 5' side and site C on the 3' side of the sequence. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. This part is in a MoClo backbone that is a modified version of the pSB1C3 backbone. See <a href: http://parts.igem.org/Part:BBa_K783058???>BBA_K783058 Summary of modifications from original part: Backbone has one added SpeI site in front of gene, BsaI sites, and 4bp fusion sites.
MoClo format of a modified version of BBa_B0030 with BC fusion sites.
This is the MoClo formatted part of a modified BBa_B0030 with BC fusion sites. See the BBa_R0010 page for full information on the part. This is a Level 0 MoClo part with flanking sites B on the 5' side and site C on the 3' side of the sequence. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. This part is in a MoClo backbone that is a modified version of the pSB1C3 backbone. See <a href: http://parts.igem.org/Part:BBa_B0030???>BBA_K783058 Summary of modifications from original part: Backbone has one added SpeI site in front of gene, BsaI sites, and 4bp fusion sites.
The MoClo format of BBa_J23118 with AB fusion sites.
===Design Notes=== This is the MoClo formatted part of BBa_J23118 with AB fusion sites. See the BBa_J23118 page for full information on the part. This is a Level 0 MoClo part with flanking sites A on the 5' side and site B on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. Summary of modifications from original part: Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See BBa_K783055 . ===Source=== iGEM Distribution Kit ===References===
The MoClo format of BBa_J23109 with AB fusion sites.
__NOTOC__ <partinfo>BBa_K1114012 short</partinfo> <partinfo>BBa_K1114012 SequenceAndFeatures</partinfo> ===Design Notes=== This is the MoClo formatted part of BBa_J23109 with AB fusion sites. See the BBa_J23109 page for full information on the part. This is a Level 0 MoClo part with flanking sites F on the 5' side and site B on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. Summary of modifications from original part: Backbone has one added SpeI site in front of gene, BsaI sites, and 4bp fusion sites. ===Source=== iGEM Distribution Kit ===References===
__NOTOC__ <partinfo>BBa_K1114033 short</partinfo> <partinfo>BBa_K1114033 SequenceAndFeatures</partinfo> ===Design Notes=== This is the MoClo formatted part of BBa_J23109 with FB fusion sites. See the BBa_J23109 page for full information on the part. This is a Level 0 MoClo part with flanking sites F on the 5' side and site B on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. Summary of modifications from original part: Backbone has one added SpeI site in front of gene, BsaI sites, and 4bp fusion sites. ===Source=== iGEM Distribution Kit ===References===
The MoClo format of BBa_J23117 with AB fusion sites.
===Design Notes=== This is the MoClo formatted part of BBa_J23117 with AB fusion sites. See the BBa_J23117 page for full information on the part. This is a Level 0 MoClo part with flanking sites A on the 5' side and site B on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. Summary of modifications from original part: Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See BBa_K783055 . ===Source=== iGEM Distribution Kit ===References===
The MoClo format of BBa_J23107 with AB fusion sites.
This is the MoClo formatted part of BBa_J23107 with AB fusion sites. See the BBa_J23107 page for full information on the part. Summary of modifications from original part: Backbone has one added SpeI site in front of gene, BsaI sites, and 4bp fusion sites. The fusion site letters refer to 4bp fusion sites: A=GGAG; B=TACT; C=AATG; D=AGGT; E=GCTT; F = CGCT; G=TGCC; H=ACTA.
A CDS of Dronpa protein with a mutation in the betha-strand 7 in Lys145 for Asn.
Pectinidae sp. sequence found in the following article Lummer, M., Humpert, F., Steuwe, C., Caesar, K., Sch??ttpelz, M., Sauer, M. and Staiger, D. (2011), Reversible Photoswitchable DRONPA-s Monitors Nucleocytoplasmic Transport of an RNA-Binding Protein in Transgenic Plants. Traffic, 12: 693???702. doi: 10.1111/j.1600-0854.2011.01180.x
The MoClo format of BBa_I13453 with AB fusion sites.
===Design Notes=== This is the MoClo formatted part of BBa_I13453 with AB fusion sites. See the BBa_I13453 page for full information on the part. This is a Level 0 MoClo part with flanking sites A on the 5' side and site B on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. Summary of modifications from original part: Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See BBa_K783055 . ===Source=== iGEM Distribution Kit ===References===
Benzalacetone Synthase, compatible with MoClo
Bicistronic design RBS 12, MoClo Format with BC fusion sites
Bicistronic design RBS 13, MoClo Format with BC fusion sites
This is the MoClo formatted part of a bicistronic design ribosomal binding site 13 with BC fusion sites. This is a Level 0 MoClo part with flanking sites B on the 5' side and site C on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See BBa_K783055.
This is a MoClo converted version of BBa_J23114
This is a MoClo converted version of BBa_J23114. It has two MoClo fusion sites flanking it.
The MoClo format of BBa_J23108 with AB fusion sites.
<partinfo> BBa_K1114011 </partinfo> <partinfo> BBa_K1114011 SequenceAndFeatures </partinfo> This is the MoClo formatted part of BBa_J23108 with AB fusion sites. See the BBa_J23108 page for full information on the part. This is a MoClo level 0 promoter with a flanking site A on the 5' side and site B on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A=GGAG; B=TACT; C=AATG; D=AGGT; E=GCTT; F = CGCT; G=TGCC; H=ACTA. Summary of modifications from original part: Backbone has one added SpeI site in front of gene, BsaI sites, and 4bp fusion sites.
It is a CDS of PhiC31 integrase from Streptomyces phage. It has a nuclear location sequence in the N-terminus.
It has a nuclear location sequence (NLS) and its codons are optimized for plants.
MoClo formatted version of B0034 with BC fusion sites
This is the MoClo formatted part of BBa_B0034 with BC fusion sites. See the BBa_B0034 page for full information on the part. This is a Level 0 MoClo part with flanking sites B on the 5' side and site C on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. Summary of modifications from original part: Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See BBa_K783055.
The MoClo format of BBa_J23103 with AB fusion sites.
MoClo format of a modified version of BBa_B0032 with BC fusion sites
This is the MoClo formatted part of a modified BBa_B0032 with BC fusion sites. See the BBa_B0032 page for full information on the part. This is a Level 0 MoClo part with flanking sites B on the 5' side and site C on the 3' side of the sequence. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. This part is in a MoClo backbone that is a modified version of the pSB1C3 backbone. See <a href: http://parts.igem.org/Part:BBa_K783058???>BBA_K783058 Summary of modifications from original part: Backbone has one added SpeI site in front of gene, BsaI sites, and 4bp fusion sites.
The MoClo format of BBa_J23105 with AB fusion sites.
This is the MoClo formatted part of BBa_J23105 with AB fusion sites. See the BBa_J23105 page for full information on the part. Summary of modifications from original part: Backbone has one added SpeI site in front of gene, BsaI sites, and 4bp fusion sites. The fusion site letters refer to 4bp fusion sites: A=GGAG; B=TACT; C=AATG; D=AGGT; E=GCTT; F = CGCT; G=TGCC; H=ACTA.