iGEM 2018 Distribution Plate 7 Well 4L
type: Collection
persistent identity: iGEM 2018 Plate7 Well4L
display id: iGEM_2018_Plate7_Well4L
version: 1
owned by: myers
member: https://synbiohub.org/public/igem/BBa_J364007/1

iGEM 2018 Distribution Plate 7 Well 5A
type: Collection
persistent identity: iGEM 2018 Plate7 Well5A
display id: iGEM_2018_Plate7_Well5A
version: 1
owned by: myers
member: BBa_K1800002

iGEM 2018 Distribution Plate 7 Well 11P
type: Collection
persistent identity: iGEM 2018 Plate7 Well11P
display id: iGEM_2018_Plate7_Well11P
version: 1
owned by: myers
member: BBa_K1610202

iGEM 2018 Distribution Plate 7 Well 18G
type: Collection
persistent identity: iGEM 2018 Plate7 Well18G
display id: iGEM_2018_Plate7_Well18G
version: 1
owned by: myers
member: BBa_K1602046

iGEM 2018 Distribution Plate 7 Well 16C
type: Collection
persistent identity: iGEM 2018 Plate7 Well16C
display id: iGEM_2018_Plate7_Well16C
version: 1
owned by: myers
member: BBa_K1602016

iGEM 2018 Distribution Plate 7 Well 21N
type: Collection
persistent identity: iGEM 2018 Plate7 Well21N
display id: iGEM_2018_Plate7_Well21N
version: 1
owned by: myers
member: https://synbiohub.org/public/igem/BBa_J364004/1

iGEM 2018 Distribution Plate 7 Well 21I
type: Collection
persistent identity: iGEM 2018 Plate7 Well21I
display id: iGEM_2018_Plate7_Well21I
version: 1
owned by: myers
member: BBa_K1789011

iGEM 2018 Distribution Plate 7 Well 15J
type: Collection
persistent identity: iGEM 2018 Plate7 Well15J
display id: iGEM_2018_Plate7_Well15J
version: 1
owned by: myers
member: BBa_K1614000

iGEM 2018 Distribution Plate 7 Well 15M
type: Collection
persistent identity: iGEM 2018 Plate7 Well15M
display id: iGEM_2018_Plate7_Well15M
version: 1
owned by: myers
member: Yeb-Art175

iGEM 2018 Distribution Plate 7 Well 21G
type: Collection
persistent identity: iGEM 2018 Plate7 Well21G
display id: iGEM_2018_Plate7_Well21G
version: 1
owned by: myers
member: BBa_K1789010

iGEM 2018 Distribution Plate 7 Well 2F
type: Collection
persistent identity: iGEM 2018 Plate7 Well2F
display id: iGEM_2018_Plate7_Well2F
version: 1
owned by: myers
member: https://synbiohub.org/public/igem/BBa_J364000/1

iGEM 2018 Distribution Plate 7 Well 9L
type: Collection
persistent identity: iGEM 2018 Plate7 Well9L
display id: iGEM_2018_Plate7_Well9L
version: 1
owned by: myers
member: BBa_K1799008

iGEM 2018 Distribution Plate 7 Well 2P
type: Collection
persistent identity: iGEM 2018 Plate7 Well2P
display id: iGEM_2018_Plate7_Well2P
version: 1
owned by: myers
member: https://synbiohub.org/public/igem/BBa_J364009/1

iGEM 2018 Distribution Plate 7 Well 11A
type: Collection
persistent identity: iGEM 2018 Plate7 Well11A
display id: iGEM_2018_Plate7_Well11A
version: 1
owned by: myers
member: BBa_K1758321

iGEM 2018 Distribution Plate 7 Well 21L
type: Collection
persistent identity: iGEM 2018 Plate7 Well21L
display id: iGEM_2018_Plate7_Well21L
version: 1
owned by: myers
member: https://synbiohub.org/public/igem/BBa_J364003/1

iGEM 2018 Distribution Plate 7 Well 4N
type: Collection
persistent identity: iGEM 2018 Plate7 Well4N
display id: iGEM_2018_Plate7_Well4N
version: 1
owned by: myers
member: https://synbiohub.org/public/igem/BBa_J364008/1

iGEM 2018 Distribution Plate 7 Well 15I
type: Collection
persistent identity: iGEM 2018 Plate7 Well15I
display id: iGEM_2018_Plate7_Well15I
version: 1
owned by: myers
member: DsbDspBx

iGEM 2018 Distribution Plate 7 Well 11N
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persistent identity: iGEM 2018 Plate7 Well11N
display id: iGEM_2018_Plate7_Well11N
version: 1
owned by: myers
member: BBa_K1799019

iGEM 2018 Distribution Plate 7 Well 9A
type: Collection
persistent identity: iGEM 2018 Plate7 Well9A
display id: iGEM_2018_Plate7_Well9A
version: 1
owned by: myers
member: BBa_K1758120

iGEM 2018 Distribution Plate 7 Well 13N
type: Collection
persistent identity: iGEM 2018 Plate7 Well13N
display id: iGEM_2018_Plate7_Well13N
version: 1
owned by: myers
member: BBa_K1795018

iGEM 2018 Distribution Plate 7 Well 9N
type: Collection
persistent identity: iGEM 2018 Plate7 Well9N
display id: iGEM_2018_Plate7_Well9N
version: 1
owned by: myers
member: BBa_K1799009

iGEM 2018 Distribution Plate 7 Well 19E
type: Collection
persistent identity: iGEM 2018 Plate7 Well19E
display id: iGEM_2018_Plate7_Well19E
version: 1
owned by: myers
member: BBa_K1632018

iGEM 2018 Distribution Plate 7 Well 13J
type: Collection
persistent identity: iGEM 2018 Plate7 Well13J
display id: iGEM_2018_Plate7_Well13J
version: 1
owned by: myers
member: BBa_K1795010

iGEM 2018 Distribution Plate 7 Well 2H
type: Collection
persistent identity: iGEM 2018 Plate7 Well2H
display id: iGEM_2018_Plate7_Well2H
version: 1
owned by: myers
member: https://synbiohub.org/public/igem/BBa_J364001/1

iGEM 2018 Distribution Plate 7 Well 15P
type: Collection
persistent identity: iGEM 2018 Plate7 Well15P
display id: iGEM_2018_Plate7_Well15P
version: 1
owned by: myers
member: BBa_K1692033

iGEM 2018 Distribution Plate 7 Well 17M
type: Collection
persistent identity: iGEM 2018 Plate7 Well17M
display id: iGEM_2018_Plate7_Well17M
version: 1
owned by: myers
member: BBa_K1632003

iGEM 2018 Distribution Plate 7 Well 21K
type: Collection
persistent identity: iGEM 2018 Plate7 Well21K
display id: iGEM_2018_Plate7_Well21K
version: 1
owned by: myers
member: BBa_K1789012

iGEM 2018 Distribution Plate 7 Well 7F
type: Collection
persistent identity: iGEM 2018 Plate7 Well7F
display id: iGEM_2018_Plate7_Well7F
version: 1
owned by: myers
member: BBa_K1847010

iGEM 2018 Distribution Plate 7 Well 23O
type: Collection
persistent identity: iGEM 2018 Plate7 Well23O
display id: iGEM_2018_Plate7_Well23O
version: 1
owned by: myers
member: BBa_J04450

iGEM 2018 Distribution Plate 7 Well 15L
type: Collection
persistent identity: iGEM 2018 Plate7 Well15L
display id: iGEM_2018_Plate7_Well15L
version: 1
owned by: myers
member: BBa_K1614001

iGEM 2018 Distribution Plate 7 Well 5F
type: Collection
persistent identity: iGEM 2018 Plate7 Well5F
display id: iGEM_2018_Plate7_Well5F
version: 1
owned by: myers
member: BBa_K1583112

iGEM 2018 Distribution Plate 7 Well 17F
type: Collection
persistent identity: iGEM 2018 Plate7 Well17F
display id: iGEM_2018_Plate7_Well17F
version: 1
owned by: myers
member: BBa_K1829007

iGEM 2018 Distribution Plate 7 Well 20O
type: Collection
persistent identity: iGEM 2018 Plate7 Well20O
display id: iGEM_2018_Plate7_Well20O
version: 1
owned by: myers
member: BBa_K1689000

iGEM 2018 Distribution Plate 7 Well 24I
type: Collection
persistent identity: iGEM 2018 Plate7 Well24I
display id: iGEM_2018_Plate7_Well24I
version: 1
owned by: myers
member: BBa_K1592000

iGEM 2018 Distribution Plate 7 Well 15E
type: Collection
persistent identity: iGEM 2018 Plate7 Well15E
display id: iGEM_2018_Plate7_Well15E
version: 1
owned by: myers
member: DspBx

iGEM 2018 Distribution Plate 7 Well 13I
type: Collection
persistent identity: iGEM 2018 Plate7 Well13I
display id: iGEM_2018_Plate7_Well13I
version: 1
owned by: myers
member: BBa_K1758376

iGEM 2018 Distribution Plate 7 Well 7I
type: Collection
persistent identity: iGEM 2018 Plate7 Well7I
display id: iGEM_2018_Plate7_Well7I
version: 1
owned by: myers
member: BBa_K1582029

iGEM 2018 Distribution Plate 7 Well 4P
type: Collection
persistent identity: iGEM 2018 Plate7 Well4P
display id: iGEM_2018_Plate7_Well4P
version: 1
owned by: myers
member: https://synbiohub.org/public/igem/BBa_J364009/1

iGEM 2018 Distribution Plate 7 Well 2N
type: Collection
persistent identity: iGEM 2018 Plate7 Well2N
display id: iGEM_2018_Plate7_Well2N
version: 1
owned by: myers
member: https://synbiohub.org/public/igem/BBa_J364008/1

iGEM 2018 Distribution Plate 7 Well 22A
type: Collection
persistent identity: iGEM 2018 Plate7 Well22A
display id: iGEM_2018_Plate7_Well22A
version: 1
owned by: myers
member: BBa_K1689001

iGEM 2018 Distribution Plate 7 Well 13P
type: Collection
persistent identity: iGEM 2018 Plate7 Well13P
display id: iGEM_2018_Plate7_Well13P
version: 1
owned by: myers
member: BBa_K1795024

iGEM 2018 Distribution Plate 7 Well 23K
type: Collection
persistent identity: iGEM 2018 Plate7 Well23K
display id: iGEM_2018_Plate7_Well23K
version: 1
owned by: myers
member: BBa_K1806003

iGEM 2018 Distribution Plate 7 Well 17B
type: Collection
persistent identity: iGEM 2018 Plate7 Well17B
display id: iGEM_2018_Plate7_Well17B
version: 1
owned by: myers
member: BBa_K1692023

iGEM 2018 Distribution Plate 7 Well 7H
type: Collection
persistent identity: iGEM 2018 Plate7 Well7H
display id: iGEM_2018_Plate7_Well7H
version: 1
owned by: myers
member: BBa_K1847011

iGEM 2018 Distribution Plate 7 Well 13D
type: Collection
persistent identity: iGEM 2018 Plate7 Well13D
display id: iGEM_2018_Plate7_Well13D
version: 1
owned by: myers
member: BBa_K1795004

iGEM 2018 Distribution Plate 7 Well 7G
type: Collection
persistent identity: iGEM 2018 Plate7 Well7G
display id: iGEM_2018_Plate7_Well7G
version: 1
owned by: myers
member: BBa_K1724000

iGEM 2018 Distribution Plate 7 Well 20G
type: Collection
persistent identity: iGEM 2018 Plate7 Well20G
display id: iGEM_2018_Plate7_Well20G
version: 1
owned by: myers
member: eYFP_SH3li

iGEM 2018 Distribution Plate 7 Well 2L
type: Collection
persistent identity: iGEM 2018 Plate7 Well2L
display id: iGEM_2018_Plate7_Well2L
version: 1
owned by: myers
member: https://synbiohub.org/public/igem/BBa_J364007/1

iGEM 2018 Distribution Plate 7 Well 1J
type: Collection
persistent identity: iGEM 2018 Plate7 Well1J
display id: iGEM_2018_Plate7_Well1J
version: 1
owned by: myers
member: Si-tag2

iGEM 2018 Distribution Plate 7 Well 5K
type: Collection
persistent identity: iGEM 2018 Plate7 Well5K
display id: iGEM_2018_Plate7_Well5K
version: 1
owned by: myers
member: BBa_K1585310

iGEM 2018 Distribution Plate 7 Well 22K
type: Collection
persistent identity: iGEM 2018 Plate7 Well22K
display id: iGEM_2018_Plate7_Well22K
version: 1
owned by: myers
member: BBa_K1689012

iGEM 2018 Distribution Plate 7 Well 10G
type: Collection
persistent identity: iGEM 2018 Plate7 Well10G
display id: iGEM_2018_Plate7_Well10G
version: 1
owned by: myers
member: BBa_K1696005

iGEM 2018 Distribution Plate 7 Well 2O
type: Collection
persistent identity: iGEM 2018 Plate7 Well2O
display id: iGEM_2018_Plate7_Well2O
version: 1
owned by: myers
member: BBa_K1639001

iGEM 2018 Distribution Plate 7 Well 15K
type: Collection
persistent identity: iGEM 2018 Plate7 Well15K
display id: iGEM_2018_Plate7_Well15K
version: 1
owned by: myers
member: Dsb-Art175

iGEM 2018 Distribution Plate 7 Well 2J
type: Collection
persistent identity: iGEM 2018 Plate7 Well2J
display id: iGEM_2018_Plate7_Well2J
version: 1
owned by: myers
member: https://synbiohub.org/public/igem/BBa_J364002/1

iGEM 2018 Distribution Plate 7 Well 3H
type: Collection
persistent identity: iGEM 2018 Plate7 Well3H
display id: iGEM_2018_Plate7_Well3H
version: 1
owned by: myers
member: BBa_K1592017

iGEM 2018 Distribution Plate 7 Well 13K
type: Collection
persistent identity: iGEM 2018 Plate7 Well13K
display id: iGEM_2018_Plate7_Well13K
version: 1
owned by: myers
member: BBa_K1758377

iGEM 2018 Distribution Plate 7 Well 21J
type: Collection
persistent identity: iGEM 2018 Plate7 Well21J
display id: iGEM_2018_Plate7_Well21J
version: 1
owned by: myers
member: https://synbiohub.org/public/igem/BBa_J364002/1

iGEM 2018 Distribution Plate 7 Well 18O
type: Collection
persistent identity: iGEM 2018 Plate7 Well18O
display id: iGEM_2018_Plate7_Well18O
version: 1
owned by: myers
member: BBa_K1602055

iGEM 2018 Distribution Plate 7 Well 17A
type: Collection
persistent identity: iGEM 2018 Plate7 Well17A
display id: iGEM_2018_Plate7_Well17A
version: 1
owned by: myers
member: Art175

iGEM 2018 Distribution Plate 7 Well 5G
type: Collection
persistent identity: iGEM 2018 Plate7 Well5G
display id: iGEM_2018_Plate7_Well5G
version: 1
owned by: myers
member: BBa_K1585212

iGEM 2018 Distribution Plate 7 Well 7M
type: Collection
persistent identity: iGEM 2018 Plate7 Well7M
display id: iGEM_2018_Plate7_Well7M
version: 1
owned by: myers
member: BBa_K1758106

iGEM 2018 Distribution Plate 7 Well 1F
type: Collection
persistent identity: iGEM 2018 Plate7 Well1F
display id: iGEM_2018_Plate7_Well1F
version: 1
owned by: myers
member: Php4d

iGEM 2018 Distribution Plate 7 Well 1L
type: Collection
persistent identity: iGEM 2018 Plate7 Well1L
display id: iGEM_2018_Plate7_Well1L
version: 1
owned by: myers
member: Si-tag3

iGEM 2018 Distribution Plate 7 Well 18C
type: Collection
persistent identity: iGEM 2018 Plate7 Well18C
display id: iGEM_2018_Plate7_Well18C
version: 1
owned by: myers
member: BBa_K1602043

iGEM 2018 Distribution Plate 7 Well 17O
type: Collection
persistent identity: iGEM 2018 Plate7 Well17O
display id: iGEM_2018_Plate7_Well17O
version: 1
owned by: myers
member: BBa_K1632008

iGEM 2018 Distribution Plate 7 Well 13A
type: Collection
persistent identity: iGEM 2018 Plate7 Well13A
display id: iGEM_2018_Plate7_Well13A
version: 1
owned by: myers
member: BBa_K1758343

iGEM 2018 Distribution Plate 7 Well 16A
type: Collection
persistent identity: iGEM 2018 Plate7 Well16A
display id: iGEM_2018_Plate7_Well16A
version: 1
owned by: myers
member: BBa_K1602014

iGEM 2018 Distribution Plate 7 Well 19G
type: Collection
persistent identity: iGEM 2018 Plate7 Well19G
display id: iGEM_2018_Plate7_Well19G
version: 1
owned by: myers
member: BBa_K1789000

iGEM 2018 Distribution Plate 7 Well 4C
type: Collection
persistent identity: iGEM 2018 Plate7 Well4C
display id: iGEM_2018_Plate7_Well4C
version: 1
owned by: myers
member: mLacI

iGEM 2018 Distribution Plate 7 Well 12K
type: Collection
persistent identity: iGEM 2018 Plate7 Well12K
display id: iGEM_2018_Plate7_Well12K
version: 1
owned by: myers
member: BBa_K1614016

iGEM 2018 Distribution Plate 7 Well 2A
type: Collection
persistent identity: iGEM 2018 Plate7 Well2A
display id: iGEM_2018_Plate7_Well2A
version: 1
owned by: myers
member: BBa_K1806004

iGEM 2018 Distribution Plate 7 Well 4B
type: Collection
persistent identity: iGEM 2018 Plate7 Well4B
display id: iGEM_2018_Plate7_Well4B
version: 1
owned by: myers
member: BBa_I20270

iGEM 2018 Distribution Plate 7 Well 11L
type: Collection
persistent identity: iGEM 2018 Plate7 Well11L
display id: iGEM_2018_Plate7_Well11L
version: 1
owned by: myers
member: BBa_K1799016

iGEM 2018 Distribution Plate 7 Well 22E
type: Collection
persistent identity: iGEM 2018 Plate7 Well22E
display id: iGEM_2018_Plate7_Well22E
version: 1
owned by: myers
member: BBa_K1689005

iGEM 2018 Distribution Plate 7 Well 7D
type: Collection
persistent identity: iGEM 2018 Plate7 Well7D
display id: iGEM_2018_Plate7_Well7D
version: 1
owned by: myers
member: BBa_K1847008

iGEM 2018 Distribution Plate 7 Well 1G
type: Collection
persistent identity: iGEM 2018 Plate7 Well1G
display id: iGEM_2018_Plate7_Well1G
version: 1
owned by: myers
member: BBa_K1763009

iGEM 2018 Distribution Plate 7 Well 13F
type: Collection
persistent identity: iGEM 2018 Plate7 Well13F
display id: iGEM_2018_Plate7_Well13F
version: 1
owned by: myers
member: BBa_K1795006

iGEM 2018 Distribution Plate 7 Well 3K
type: Collection
persistent identity: iGEM 2018 Plate7 Well3K
display id: iGEM_2018_Plate7_Well3K
version: 1
owned by: myers
member: BBa_K1699003

iGEM 2018 Distribution Plate 7 Well 24A
type: Collection
persistent identity: iGEM 2018 Plate7 Well24A
display id: iGEM_2018_Plate7_Well24A
version: 1
owned by: myers
member: BBa_K1689015

iGEM 2018 Distribution Plate 7 Well 9D
type: Collection
persistent identity: iGEM 2018 Plate7 Well9D
display id: iGEM_2018_Plate7_Well9D
version: 1
owned by: myers
member: BBa_K1799000

iGEM 2018 Distribution Plate 7 Well 5I
type: Collection
persistent identity: iGEM 2018 Plate7 Well5I
display id: iGEM_2018_Plate7_Well5I
version: 1
owned by: myers
member: BBa_K1585213

iGEM 2018 Distribution Plate 7 Well 13O
type: Collection
persistent identity: iGEM 2018 Plate7 Well13O
display id: iGEM_2018_Plate7_Well13O
version: 1
owned by: myers
member: BBa_K1659501

iGEM 2018 Distribution Plate 7 Well 20I
type: Collection
persistent identity: iGEM 2018 Plate7 Well20I
display id: iGEM_2018_Plate7_Well20I
version: 1
owned by: myers
member: eYFP_SH3 g

iGEM 2018 Distribution Plate 7 Well 18E
type: Collection
persistent identity: iGEM 2018 Plate7 Well18E
display id: iGEM_2018_Plate7_Well18E
version: 1
owned by: myers
member: BBa_K1602044

iGEM 2018 Distribution Plate 7 Well 2C
type: Collection
persistent identity: iGEM 2018 Plate7 Well2C
display id: iGEM_2018_Plate7_Well2C
version: 1
owned by: myers
member: BBa_K1806005

iGEM 2018 Distribution Plate 7 Well 20C
type: Collection
persistent identity: iGEM 2018 Plate7 Well20C
display id: iGEM_2018_Plate7_Well20C
version: 1
owned by: myers
member: BBa_K1602058

iGEM 2018 Distribution Plate 7 Well 1O
type: Collection
persistent identity: iGEM 2018 Plate7 Well1O
display id: iGEM_2018_Plate7_Well1O
version: 1
owned by: myers
member: BBa_K1692001

iGEM 2018 Distribution Plate 7 Well 5N
type: Collection
persistent identity: iGEM 2018 Plate7 Well5N
display id: iGEM_2018_Plate7_Well5N
version: 1
owned by: myers
member: BBa_K1847003

iGEM 2018 Distribution Plate 7 Well 3P
type: Collection
persistent identity: iGEM 2018 Plate7 Well3P
display id: iGEM_2018_Plate7_Well3P
version: 1
owned by: myers
member: BBa_K1690003

iGEM 2018 Distribution Plate 7 Well 15F
type: Collection
persistent identity: iGEM 2018 Plate7 Well15F
display id: iGEM_2018_Plate7_Well15F
version: 1
owned by: myers
member: BBa_K1614019

iGEM 2018 Distribution Plate 7 Well 9O
type: Collection
persistent identity: iGEM 2018 Plate7 Well9O
display id: iGEM_2018_Plate7_Well9O
version: 1
owned by: myers
member: BBa_K1758314

iGEM 2018 Distribution Plate 7 Well 4M
type: Collection
persistent identity: iGEM 2018 Plate7 Well4M
display id: iGEM_2018_Plate7_Well4M
version: 1
owned by: myers
member: BBa_K1763425

iGEM 2018 Distribution Plate 7 Well 4A
type: Collection
persistent identity: iGEM 2018 Plate7 Well4A
display id: iGEM_2018_Plate7_Well4A
version: 1
owned by: myers
member: BBa_K1639004

iGEM 2018 Distribution Plate 7 Well 14I
type: Collection
persistent identity: iGEM 2018 Plate7 Well14I
display id: iGEM_2018_Plate7_Well14I
version: 1
owned by: myers
member: BBa_K1602010

iGEM 2018 Distribution Plate 7 Well 12E
type: Collection
persistent identity: iGEM 2018 Plate7 Well12E
display id: iGEM_2018_Plate7_Well12E
version: 1
owned by: myers
member: BBa_K1640012

iGEM 2018 Distribution Plate 7 Well 17K
type: Collection
persistent identity: iGEM 2018 Plate7 Well17K
display id: iGEM_2018_Plate7_Well17K
version: 1
owned by: myers
member: BBa_K1604022

iGEM 2018 Distribution Plate 7 Well 6O
type: Collection
persistent identity: iGEM 2018 Plate7 Well6O
display id: iGEM_2018_Plate7_Well6O
version: 1
owned by: myers
member: BBa_K1763442

iGEM 2018 Distribution Plate 7 Well 13E
type: Collection
persistent identity: iGEM 2018 Plate7 Well13E
display id: iGEM_2018_Plate7_Well13E
version: 1
owned by: myers
member: BBa_K1758361

iGEM 2018 Distribution Plate 7 Well 20E
type: Collection
persistent identity: iGEM 2018 Plate7 Well20E
display id: iGEM_2018_Plate7_Well20E
version: 1
owned by: myers
member: eYFP_GBDli

iGEM 2018 Distribution Plate 7 Well 15D
type: Collection
persistent identity: iGEM 2018 Plate7 Well15D
display id: iGEM_2018_Plate7_Well15D
version: 1
owned by: myers
member: BBa_K1614018

iGEM 2018 Distribution Plate 7 Well 9F
type: Collection
persistent identity: iGEM 2018 Plate7 Well9F
display id: iGEM_2018_Plate7_Well9F
version: 1
owned by: myers
member: BBa_K1799002

iGEM 2018 Distribution Plate 7 Well 1B
type: Collection
persistent identity: iGEM 2018 Plate7 Well1B
display id: iGEM_2018_Plate7_Well1B
version: 1
owned by: myers
member: YLcwp3

iGEM 2018 Distribution Plate 7 Well 23I
type: Collection
persistent identity: iGEM 2018 Plate7 Well23I
display id: iGEM_2018_Plate7_Well23I
version: 1
owned by: myers
member: BBa_K1806001

iGEM 2018 Distribution Plate 7 Well 10K
type: Collection
persistent identity: iGEM 2018 Plate7 Well10K
display id: iGEM_2018_Plate7_Well10K
version: 1
owned by: myers
member: BBa_K1696013

iGEM 2018 Distribution Plate 7 Well 14G
type: Collection
persistent identity: iGEM 2018 Plate7 Well14G
display id: iGEM_2018_Plate7_Well14G
version: 1
owned by: myers
member: BBa_K1602009

iGEM 2018 Distribution Plate 7 Well 11H
type: Collection
persistent identity: iGEM 2018 Plate7 Well11H
display id: iGEM_2018_Plate7_Well11H
version: 1
owned by: myers
member: BBa_K1799014

iGEM 2018 Distribution Plate 7 Well 5J
type: Collection
persistent identity: iGEM 2018 Plate7 Well5J
display id: iGEM_2018_Plate7_Well5J
version: 1
owned by: myers
member: BBa_K1583113

iGEM 2018 Distribution Plate 7 Well 23P
type: Collection
persistent identity: iGEM 2018 Plate7 Well23P
display id: iGEM_2018_Plate7_Well23P
version: 1
owned by: myers
member: BBa_J04450

iGEM 2018 Distribution Plate 7 Well 3N
type: Collection
persistent identity: iGEM 2018 Plate7 Well3N
display id: iGEM_2018_Plate7_Well3N
version: 1
owned by: myers
member: BBa_K1680009

iGEM 2018 Distribution Plate 7 Well 6E
type: Collection
persistent identity: iGEM 2018 Plate7 Well6E
display id: iGEM_2018_Plate7_Well6E
version: 1
owned by: myers
member: BBa_K1763435

iGEM 2018 Distribution Plate 7 Well 9B
type: Collection
persistent identity: iGEM 2018 Plate7 Well9B
display id: iGEM_2018_Plate7_Well9B
version: 1
owned by: myers
member: BBa_K1723005

iGEM 2018 Distribution Plate 7 Well 24C
type: Collection
persistent identity: iGEM 2018 Plate7 Well24C
display id: iGEM_2018_Plate7_Well24C
version: 1
owned by: myers
member: BBa_K1689016

iGEM 2018 Distribution Plate 7 Well 19C
type: Collection
persistent identity: iGEM 2018 Plate7 Well19C
display id: iGEM_2018_Plate7_Well19C
version: 1
owned by: myers
member: BBa_K1632013

iGEM 2018 Distribution Plate 7 Well 23G
type: Collection
persistent identity: iGEM 2018 Plate7 Well23G
display id: iGEM_2018_Plate7_Well23G
version: 1
owned by: myers
member: BBa_K1632011

iGEM 2018 Distribution Plate 7 Well 7O
type: Collection
persistent identity: iGEM 2018 Plate7 Well7O
display id: iGEM_2018_Plate7_Well7O
version: 1
owned by: myers
member: BBa_K1758107

iGEM 2018 Distribution Plate 7 Well 6M
type: Collection
persistent identity: iGEM 2018 Plate7 Well6M
display id: iGEM_2018_Plate7_Well6M
version: 1
owned by: myers
member: BBa_K1763441

iGEM 2018 Distribution Plate 7 Well 17I
type: Collection
persistent identity: iGEM 2018 Plate7 Well17I
display id: iGEM_2018_Plate7_Well17I
version: 1
owned by: myers
member: BBa_K1604021

iGEM 2018 Distribution Plate 7 Well 8C
type: Collection
persistent identity: iGEM 2018 Plate7 Well8C
display id: iGEM_2018_Plate7_Well8C
version: 1
owned by: myers
member: BBa_K1763007

iGEM 2018 Distribution Plate 7 Well 2D
type: Collection
persistent identity: iGEM 2018 Plate7 Well2D
display id: iGEM_2018_Plate7_Well2D
version: 1
owned by: myers
member: p(tetR)

iGEM 2018 Distribution Plate 7 Well 5E
type: Collection
persistent identity: iGEM 2018 Plate7 Well5E
display id: iGEM_2018_Plate7_Well5E
version: 1
owned by: myers
member: BBa_K1585211

iGEM 2018 Distribution Plate 7 Well 7L
type: Collection
persistent identity: iGEM 2018 Plate7 Well7L
display id: iGEM_2018_Plate7_Well7L
version: 1
owned by: myers
member: BBa_K1595001

iGEM 2018 Distribution Plate 7 Well 11M
type: Collection
persistent identity: iGEM 2018 Plate7 Well11M
display id: iGEM_2018_Plate7_Well11M
version: 1
owned by: myers
member: BBa_K1758335

iGEM 2018 Distribution Plate 7 Well 22G
type: Collection
persistent identity: iGEM 2018 Plate7 Well22G
display id: iGEM_2018_Plate7_Well22G
version: 1
owned by: myers
member: BBa_K1689006

iGEM 2018 Distribution Plate 7 Well 4O
type: Collection
persistent identity: iGEM 2018 Plate7 Well4O
display id: iGEM_2018_Plate7_Well4O
version: 1
owned by: myers
member: BBa_K1763426

iGEM 2018 Distribution Plate 7 Well 4E
type: Collection
persistent identity: iGEM 2018 Plate7 Well4E
display id: iGEM_2018_Plate7_Well4E
version: 1
owned by: myers
member: mLacI

iGEM 2018 Distribution Plate 7 Well 6C
type: Collection
persistent identity: iGEM 2018 Plate7 Well6C
display id: iGEM_2018_Plate7_Well6C
version: 1
owned by: myers
member: BBa_K1763434

iGEM 2018 Distribution Plate 7 Well 11J
type: Collection
persistent identity: iGEM 2018 Plate7 Well11J
display id: iGEM_2018_Plate7_Well11J
version: 1
owned by: myers
member: BBa_K1799015

iGEM 2018 Distribution Plate 7 Well 10O
type: Collection
persistent identity: iGEM 2018 Plate7 Well10O
display id: iGEM_2018_Plate7_Well10O
version: 1
owned by: myers
member: BBa_K1640018

iGEM 2018 Distribution Plate 7 Well 1N
type: Collection
persistent identity: iGEM 2018 Plate7 Well1N
display id: iGEM_2018_Plate7_Well1N
version: 1
owned by: myers
member: Si-tag1+2

iGEM 2018 Distribution Plate 7 Well 16E
type: Collection
persistent identity: iGEM 2018 Plate7 Well16E
display id: iGEM_2018_Plate7_Well16E
version: 1
owned by: myers
member: HIC genera

iGEM 2018 Distribution Plate 7 Well 5L
type: Collection
persistent identity: iGEM 2018 Plate7 Well5L
display id: iGEM_2018_Plate7_Well5L
version: 1
owned by: myers
member: BBa_K1847002

iGEM 2018 Distribution Plate 7 Well 24E
type: Collection
persistent identity: iGEM 2018 Plate7 Well24E
display id: iGEM_2018_Plate7_Well24E
version: 1
owned by: myers
member: BBa_K1689017

iGEM 2018 Distribution Plate 7 Well 3I
type: Collection
persistent identity: iGEM 2018 Plate7 Well3I
display id: iGEM_2018_Plate7_Well3I
version: 1
owned by: myers
member: BBa_K1699002

iGEM 2018 Distribution Plate 7 Well 16G
type: Collection
persistent identity: iGEM 2018 Plate7 Well16G
display id: iGEM_2018_Plate7_Well16G
version: 1
owned by: myers
member: RBS-GFP_Si

iGEM 2018 Distribution Plate 7 Well 21P
type: Collection
persistent identity: iGEM 2018 Plate7 Well21P
display id: iGEM_2018_Plate7_Well21P
version: 1
owned by: myers
member: https://synbiohub.org/public/igem/BBa_J364005/1

iGEM 2018 Distribution Plate 7 Well 7N
type: Collection
persistent identity: iGEM 2018 Plate7 Well7N
display id: iGEM_2018_Plate7_Well7N
version: 1
owned by: myers
member: BBa_K1595006

iGEM 2018 Distribution Plate 7 Well 1D
type: Collection
persistent identity: iGEM 2018 Plate7 Well1D
display id: iGEM_2018_Plate7_Well1D
version: 1
owned by: myers
member: BBa_K1592003

iGEM 2018 Distribution Plate 7 Well 20A
type: Collection
persistent identity: iGEM 2018 Plate7 Well20A
display id: iGEM_2018_Plate7_Well20A
version: 1
owned by: myers
member: BBa_K1602056

iGEM 2018 Distribution Plate 7 Well 3J
type: Collection
persistent identity: iGEM 2018 Plate7 Well3J
display id: iGEM_2018_Plate7_Well3J
version: 1
owned by: myers
member: BBa_K1592020

iGEM 2018 Distribution Plate 7 Well 3B
type: Collection
persistent identity: iGEM 2018 Plate7 Well3B
display id: iGEM_2018_Plate7_Well3B
version: 1
owned by: myers
member: Si-tag1+3

iGEM 2018 Distribution Plate 7 Well 13M
type: Collection
persistent identity: iGEM 2018 Plate7 Well13M
display id: iGEM_2018_Plate7_Well13M
version: 1
owned by: myers
member: BBa_K1583100

iGEM 2018 Distribution Plate 7 Well 8K
type: Collection
persistent identity: iGEM 2018 Plate7 Well8K
display id: iGEM_2018_Plate7_Well8K
version: 1
owned by: myers
member: BBa_K1850010

iGEM 2018 Distribution Plate 7 Well 3A
type: Collection
persistent identity: iGEM 2018 Plate7 Well3A
display id: iGEM_2018_Plate7_Well3A
version: 1
owned by: myers
member: BBa_K1692003

iGEM 2018 Distribution Plate 7 Well 4I
type: Collection
persistent identity: iGEM 2018 Plate7 Well4I
display id: iGEM_2018_Plate7_Well4I
version: 1
owned by: myers
member: BBa_K1763423

iGEM 2018 Distribution Plate 7 Well 11F
type: Collection
persistent identity: iGEM 2018 Plate7 Well11F
display id: iGEM_2018_Plate7_Well11F
version: 1
owned by: myers
member: BBa_K1799013

iGEM 2018 Distribution Plate 7 Well 10C
type: Collection
persistent identity: iGEM 2018 Plate7 Well10C
display id: iGEM_2018_Plate7_Well10C
version: 1
owned by: myers
member: ldhE-Lc

iGEM 2018 Distribution Plate 7 Well 16I
type: Collection
persistent identity: iGEM 2018 Plate7 Well16I
display id: iGEM_2018_Plate7_Well16I
version: 1
owned by: myers
member: His-Scaffo

iGEM 2018 Distribution Plate 7 Well 1P
type: Collection
persistent identity: iGEM 2018 Plate7 Well1P
display id: iGEM_2018_Plate7_Well1P
version: 1
owned by: myers
member: Si-tag2+3

iGEM 2018 Distribution Plate 7 Well 16O
type: Collection
persistent identity: iGEM 2018 Plate7 Well16O
display id: iGEM_2018_Plate7_Well16O
version: 1
owned by: myers
member: aes-SH3lig

iGEM 2018 Distribution Plate 7 Well 20K
type: Collection
persistent identity: iGEM 2018 Plate7 Well20K
display id: iGEM_2018_Plate7_Well20K
version: 1
owned by: myers
member: UNIK Antif

iGEM 2018 Distribution Plate 7 Well 15G
type: Collection
persistent identity: iGEM 2018 Plate7 Well15G
display id: iGEM_2018_Plate7_Well15G
version: 1
owned by: myers
member: MccS

iGEM 2018 Distribution Plate 7 Well 9M
type: Collection
persistent identity: iGEM 2018 Plate7 Well9M
display id: iGEM_2018_Plate7_Well9M
version: 1
owned by: myers
member: BBa_K1758312

iGEM 2018 Distribution Plate 7 Well 18K
type: Collection
persistent identity: iGEM 2018 Plate7 Well18K
display id: iGEM_2018_Plate7_Well18K
version: 1
owned by: myers
member: BBa_K1602050

iGEM 2018 Distribution Plate 7 Well 7E
type: Collection
persistent identity: iGEM 2018 Plate7 Well7E
display id: iGEM_2018_Plate7_Well7E
version: 1
owned by: myers
member: tFba1

iGEM 2018 Distribution Plate 7 Well 13C
type: Collection
persistent identity: iGEM 2018 Plate7 Well13C
display id: iGEM_2018_Plate7_Well13C
version: 1
owned by: myers
member: BBa_K1758360

iGEM 2018 Distribution Plate 7 Well 11B
type: Collection
persistent identity: iGEM 2018 Plate7 Well11B
display id: iGEM_2018_Plate7_Well11B
version: 1
owned by: myers
member: BBa_K1799011

iGEM 2018 Distribution Plate 7 Well 21A
type: Collection
persistent identity: iGEM 2018 Plate7 Well21A
display id: iGEM_2018_Plate7_Well21A
version: 1
owned by: myers
member: BBa_K1789006

iGEM 2018 Distribution Plate 7 Well 3C
type: Collection
persistent identity: iGEM 2018 Plate7 Well3C
display id: iGEM_2018_Plate7_Well3C
version: 1
owned by: myers
member: BBa_K1692004

iGEM 2018 Distribution Plate 7 Well 16K
type: Collection
persistent identity: iGEM 2018 Plate7 Well16K
display id: iGEM_2018_Plate7_Well16K
version: 1
owned by: myers
member: RBS-Scaffo

iGEM 2018 Distribution Plate 7 Well 2E
type: Collection
persistent identity: iGEM 2018 Plate7 Well2E
display id: iGEM_2018_Plate7_Well2E
version: 1
owned by: myers
member: SP-aprE

iGEM 2018 Distribution Plate 7 Well 12I
type: Collection
persistent identity: iGEM 2018 Plate7 Well12I
display id: iGEM_2018_Plate7_Well12I
version: 1
owned by: myers
member: BBa_K1614002

iGEM 2018 Distribution Plate 7 Well 24G
type: Collection
persistent identity: iGEM 2018 Plate7 Well24G
display id: iGEM_2018_Plate7_Well24G
version: 1
owned by: myers
member: BBa_K1689018

iGEM 2018 Distribution Plate 7 Well 4G
type: Collection
persistent identity: iGEM 2018 Plate7 Well4G
display id: iGEM_2018_Plate7_Well4G
version: 1
owned by: myers
member: T7 express

iGEM 2018 Distribution Plate 7 Well 1I
type: Collection
persistent identity: iGEM 2018 Plate7 Well1I
display id: iGEM_2018_Plate7_Well1I
version: 1
owned by: myers
member: BBa_K1763010

iGEM 2018 Distribution Plate 7 Well 3D
type: Collection
persistent identity: iGEM 2018 Plate7 Well3D
display id: iGEM_2018_Plate7_Well3D
version: 1
owned by: myers
member: Si-tag1+li

iGEM 2018 Distribution Plate 7 Well 22I
type: Collection
persistent identity: iGEM 2018 Plate7 Well22I
display id: iGEM_2018_Plate7_Well22I
version: 1
owned by: myers
member: BBa_K1689007

iGEM 2018 Distribution Plate 7 Well 12G
type: Collection
persistent identity: iGEM 2018 Plate7 Well12G
display id: iGEM_2018_Plate7_Well12G
version: 1
owned by: myers
member: BBa_K1640020

iGEM 2018 Distribution Plate 7 Well 6K
type: Collection
persistent identity: iGEM 2018 Plate7 Well6K
display id: iGEM_2018_Plate7_Well6K
version: 1
owned by: myers
member: BBa_K1763440

iGEM 2018 Distribution Plate 7 Well 19B
type: Collection
persistent identity: iGEM 2018 Plate7 Well19B
display id: iGEM_2018_Plate7_Well19B
version: 1
owned by: myers
member: BBa_K1666005

iGEM 2018 Distribution Plate 7 Well 8I
type: Collection
persistent identity: iGEM 2018 Plate7 Well8I
display id: iGEM_2018_Plate7_Well8I
version: 1
owned by: myers
member: Tamura

iGEM 2018 Distribution Plate 7 Well 13H
type: Collection
persistent identity: iGEM 2018 Plate7 Well13H
display id: iGEM_2018_Plate7_Well13H
version: 1
owned by: myers
member: BBa_K1795007

iGEM 2018 Distribution Plate 7 Well 3M
type: Collection
persistent identity: iGEM 2018 Plate7 Well3M
display id: iGEM_2018_Plate7_Well3M
version: 1
owned by: myers
member: BBa_K1699004

iGEM 2018 Distribution Plate 7 Well 3F
type: Collection
persistent identity: iGEM 2018 Plate7 Well3F
display id: iGEM_2018_Plate7_Well3F
version: 1
owned by: myers
member: Si-tag1+2+

iGEM 2018 Distribution Plate 7 Well 7P
type: Collection
persistent identity: iGEM 2018 Plate7 Well7P
display id: iGEM_2018_Plate7_Well7P
version: 1
owned by: myers
member: BBa_K1723000

iGEM 2018 Distribution Plate 7 Well 23E
type: Collection
persistent identity: iGEM 2018 Plate7 Well23E
display id: iGEM_2018_Plate7_Well23E
version: 1
owned by: myers
member: BBa_K1632010

iGEM 2018 Distribution Plate 7 Well 19A
type: Collection
persistent identity: iGEM 2018 Plate7 Well19A
display id: iGEM_2018_Plate7_Well19A
version: 1
owned by: myers
member: BBa_K1632012

iGEM 2018 Distribution Plate 7 Well 7B
type: Collection
persistent identity: iGEM 2018 Plate7 Well7B
display id: iGEM_2018_Plate7_Well7B
version: 1
owned by: myers
member: BBa_K1847005

iGEM 2018 Distribution Plate 7 Well 17N
type: Collection
persistent identity: iGEM 2018 Plate7 Well17N
display id: iGEM_2018_Plate7_Well17N
version: 1
owned by: myers
member: BBa_K1666003

iGEM 2018 Distribution Plate 7 Well 12M
type: Collection
persistent identity: iGEM 2018 Plate7 Well12M
display id: iGEM_2018_Plate7_Well12M
version: 1
owned by: myers
member: RBS-xylB

iGEM 2018 Distribution Plate 7 Well 15H
type: Collection
persistent identity: iGEM 2018 Plate7 Well15H
display id: iGEM_2018_Plate7_Well15H
version: 1
owned by: myers
member: BBa_K1614020

iGEM 2018 Distribution Plate 7 Well 10M
type: Collection
persistent identity: iGEM 2018 Plate7 Well10M
display id: iGEM_2018_Plate7_Well10M
version: 1
owned by: myers
member: BBa_K1696015

iGEM 2018 Distribution Plate 7 Well 8E
type: Collection
persistent identity: iGEM 2018 Plate7 Well8E
display id: iGEM_2018_Plate7_Well8E
version: 1
owned by: myers
member: BBa_K1763015

iGEM 2018 Distribution Plate 7 Well 12C
type: Collection
persistent identity: iGEM 2018 Plate7 Well12C
display id: iGEM_2018_Plate7_Well12C
version: 1
owned by: myers
member: BBa_K1640006

iGEM 2018 Distribution Plate 7 Well 14E
type: Collection
persistent identity: iGEM 2018 Plate7 Well14E
display id: iGEM_2018_Plate7_Well14E
version: 1
owned by: myers
member: BBa_K1602008

iGEM 2018 Distribution Plate 7 Well 12O
type: Collection
persistent identity: iGEM 2018 Plate7 Well12O
display id: iGEM_2018_Plate7_Well12O
version: 1
owned by: myers
member: RBS-xylC

iGEM 2018 Distribution Plate 7 Well 21M
type: Collection
persistent identity: iGEM 2018 Plate7 Well21M
display id: iGEM_2018_Plate7_Well21M
version: 1
owned by: myers
member: BBa_K1632000

iGEM 2018 Distribution Plate 7 Well 1E
type: Collection
persistent identity: iGEM 2018 Plate7 Well1E
display id: iGEM_2018_Plate7_Well1E
version: 1
owned by: myers
member: BBa_K1763004

iGEM 2018 Distribution Plate 7 Well 9H
type: Collection
persistent identity: iGEM 2018 Plate7 Well9H
display id: iGEM_2018_Plate7_Well9H
version: 1
owned by: myers
member: BBa_K1799004

iGEM 2018 Distribution Plate 7 Well 18I
type: Collection
persistent identity: iGEM 2018 Plate7 Well18I
display id: iGEM_2018_Plate7_Well18I
version: 1
owned by: myers
member: BBa_K1602049

iGEM 2018 Distribution Plate 7 Well 4K
type: Collection
persistent identity: iGEM 2018 Plate7 Well4K
display id: iGEM_2018_Plate7_Well4K
version: 1
owned by: myers
member: BBa_K1763424

iGEM 2018 Distribution Plate 7 Well 8M
type: Collection
persistent identity: iGEM 2018 Plate7 Well8M
display id: iGEM_2018_Plate7_Well8M
version: 1
owned by: myers
member: BBa_K1850011

iGEM 2018 Distribution Plate 7 Well 5C
type: Collection
persistent identity: iGEM 2018 Plate7 Well5C
display id: iGEM_2018_Plate7_Well5C
version: 1
owned by: myers
member: BBa_K1653006

iGEM 2018 Distribution Plate 7 Well 5P
type: Collection
persistent identity: iGEM 2018 Plate7 Well5P
display id: iGEM_2018_Plate7_Well5P
version: 1
owned by: myers
member: BBa_K1847004

iGEM 2018 Distribution Plate 7 Well 10A
type: Collection
persistent identity: iGEM 2018 Plate7 Well10A
display id: iGEM_2018_Plate7_Well10A
version: 1
owned by: myers
member: BBa_K1692032

iGEM 2018 Distribution Plate 7 Well 7C
type: Collection
persistent identity: iGEM 2018 Plate7 Well7C
display id: iGEM_2018_Plate7_Well7C
version: 1
owned by: myers
member: tEno2

iGEM 2018 Distribution Plate 7 Well 14K
type: Collection
persistent identity: iGEM 2018 Plate7 Well14K
display id: iGEM_2018_Plate7_Well14K
version: 1
owned by: myers
member: BBa_K1602011

iGEM 2018 Distribution Plate 7 Well 7K
type: Collection
persistent identity: iGEM 2018 Plate7 Well7K
display id: iGEM_2018_Plate7_Well7K
version: 1
owned by: myers
member: BBa_K1758105

iGEM 2018 Distribution Plate 7 Well 3O
type: Collection
persistent identity: iGEM 2018 Plate7 Well3O
display id: iGEM_2018_Plate7_Well3O
version: 1
owned by: myers
member: BBa_K1699005

iGEM 2018 Distribution Plate 7 Well 15B
type: Collection
persistent identity: iGEM 2018 Plate7 Well15B
display id: iGEM_2018_Plate7_Well15B
version: 1
owned by: myers
member: BBa_K1650037

iGEM 2018 Distribution Plate 7 Well 7A
type: Collection
persistent identity: iGEM 2018 Plate7 Well7A
display id: iGEM_2018_Plate7_Well7A
version: 1
owned by: myers
member: pFig2c

iGEM 2018 Distribution Plate 7 Well 15A
type: Collection
persistent identity: iGEM 2018 Plate7 Well15A
display id: iGEM_2018_Plate7_Well15A
version: 1
owned by: myers
member: BBa_K1659301

iGEM 2018 Distribution Plate 7 Well 11K
type: Collection
persistent identity: iGEM 2018 Plate7 Well11K
display id: iGEM_2018_Plate7_Well11K
version: 1
owned by: myers
member: BBa_K1758333

iGEM 2018 Distribution Plate 7 Well 15O
type: Collection
persistent identity: iGEM 2018 Plate7 Well15O
display id: iGEM_2018_Plate7_Well15O
version: 1
owned by: myers
member: Fla-Art175

iGEM 2018 Distribution Plate 7
type: Collection
persistent identity: iGEM 2018 Plate7
display id: iGEM_2018_Plate7
version: 1
owned by: myers
member: BBa_K1592000
member: 3alpha-HSD
member: BBa_K1696009
member: iGEM 2018 Distribution Plate 7 Well 15O
member: BBa_K1847008
member: iGEM 2018 Distribution Plate 7 Well 23E
member: iGEM 2018 Distribution Plate 7 Well 9D
member: iGEM 2018 Distribution Plate 7 Well 3D
member: iGEM 2018 Distribution Plate 7 Well 18O
member: BBa_K1806005
member: iGEM 2018 Distribution Plate 7 Well 12O
member: BBa_K1763425
member: BBa_K1799011
member: BBa_K1758360
member: BBa_K1758330
member: BBa_K1585312
member: iGEM 2018 Distribution Plate 7 Well 21N
member: BBa_K1650037
member: BBa_K1758300
member: BBa_K1614002
member: BBa_K1799010
member: iGEM 2018 Distribution Plate 7 Well 8I
member: BBa_K1674002
member: BBa_K1758204
member: BBa_K1789000
member: iGEM 2018 Distribution Plate 7 Well 15N
member: iGEM 2018 Distribution Plate 7 Well 3C
member: iGEM 2018 Distribution Plate 7 Well 9C
member: BBa_K1632018
member: iGEM 2018 Distribution Plate 7 Well 6C
member: iGEM 2018 Distribution Plate 7 Well 5I
member: BBa_K1604022
member: iGEM 2018 Distribution Plate 7 Well 2I
member: BBa_K1799004
member: BBa_K1763012
member: iGEM 2018 Distribution Plate 7 Well 3E
member: SP-aprE
member: iGEM 2018 Distribution Plate 7 Well 13J
member: iGEM 2018 Distribution Plate 7 Well 15P
member: iGEM 2018 Distribution Plate 7 Well 7M
member: BBa_K1653006
member: iGEM 2018 Distribution Plate 7 Well 15B
member: BBa_K1789002
member: iGEM 2018 Distribution Plate 7 Well 4M
member: iGEM 2018 Distribution Plate 7 Well 8G
member: iGEM 2018 Distribution Plate 7 Well 21B
member: iGEM 2018 Distribution Plate 7 Well 5G
member: BBa_K1585310
member: iGEM 2018 Distribution Plate 7 Well 2G
member: iGEM 2018 Distribution Plate 7 Well 1M
member: BBa_K1795006
member: RBS-xylB
member: iGEM 2018 Distribution Plate 7 Well 17F
member: BBa_K1763423
member: iGEM 2018 Distribution Plate 7 Well 13L
member: iGEM 2018 Distribution Plate 7 Well 11F
member: iGEM 2018 Distribution Plate 7 Well 7K
member: iGEM 2018 Distribution Plate 7 Well 4K
member: pBar1
member: iGEM 2018 Distribution Plate 7 Well 23G
member: iGEM 2018 Distribution Plate 7 Well 20G
member: iGEM 2018 Distribution Plate 7 Well 9B
member: BBa_K1795010
member: BBa_K1602046
member: BBa_K1692023
member: iGEM 2018 Distribution Plate 7 Well 21P
member: BBa_K1602016
member: iGEM 2018 Distribution Plate 7 Well 13I
member: iGEM 2018 Distribution Plate 7 Well 10I
member: iGEM 2018 Distribution Plate 7 Well 1K
member: BBa_K1604021
member: BBa_K1799012
member: iGEM 2018 Distribution Plate 7 Well 17D
member: iGEM 2018 Distribution Plate 7 Well 11D

iGEM 2018 Distribution Plate 7 Well 2B
type: Collection
persistent identity: iGEM 2018 Plate7 Well2B
display id: iGEM_2018_Plate7_Well2B
version: 1
owned by: myers
member: BBa_I20270

iGEM 2018 Distribution Plate 7 Well 17P
type: Collection
persistent identity: iGEM 2018 Plate7 Well17P
display id: iGEM_2018_Plate7_Well17P
version: 1
owned by: myers
member: BBa_K1666004

iGEM 2018 Distribution Plate 7 Well 2G
type: Collection
persistent identity: iGEM 2018 Plate7 Well2G
display id: iGEM_2018_Plate7_Well2G
version: 1
owned by: myers
member: 3alpha-HSD

iGEM 2018 Distribution Plate 7 Well 11D
type: Collection
persistent identity: iGEM 2018 Plate7 Well11D
display id: iGEM_2018_Plate7_Well11D
version: 1
owned by: myers
member: BBa_K1799012

iGEM 2018 Distribution Plate 7 Well 21O
type: Collection
persistent identity: iGEM 2018 Plate7 Well21O
display id: iGEM_2018_Plate7_Well21O
version: 1
owned by: myers
member: BBa_K1632001

iGEM 2018 Distribution Plate 7 Well 21E
type: Collection
persistent identity: iGEM 2018 Plate7 Well21E
display id: iGEM_2018_Plate7_Well21E
version: 1
owned by: myers
member: BBa_K1789009

iGEM 2018 Distribution Plate 7 Well 5O
type: Collection
persistent identity: iGEM 2018 Plate7 Well5O
display id: iGEM_2018_Plate7_Well5O
version: 1
owned by: myers
member: pBar1

iGEM 2018 Distribution Plate 7 Well 11E
type: Collection
persistent identity: iGEM 2018 Plate7 Well11E
display id: iGEM_2018_Plate7_Well11E
version: 1
owned by: myers
member: BBa_K1758325

iGEM 2018 Distribution Plate 7 Well 9K
type: Collection
persistent identity: iGEM 2018 Plate7 Well9K
display id: iGEM_2018_Plate7_Well9K
version: 1
owned by: myers
member: BBa_K1758311

iGEM 2018 Distribution Plate 7 Well 17G
type: Collection
persistent identity: iGEM 2018 Plate7 Well17G
display id: iGEM_2018_Plate7_Well17G
version: 1
owned by: myers
member: BBa_K1598003

iGEM 2018 Distribution Plate 7 Well 14M
type: Collection
persistent identity: iGEM 2018 Plate7 Well14M
display id: iGEM_2018_Plate7_Well14M
version: 1
owned by: myers
member: BBa_K1602012

iGEM 2018 Distribution Plate 7 Well 4D
type: Collection
persistent identity: iGEM 2018 Plate7 Well4D
display id: iGEM_2018_Plate7_Well4D
version: 1
owned by: myers
member: p(tetR)

iGEM 2018 Distribution Plate 7 Well 3L
type: Collection
persistent identity: iGEM 2018 Plate7 Well3L
display id: iGEM_2018_Plate7_Well3L
version: 1
owned by: myers
member: BBa_K1682004

iGEM 2018 Distribution Plate 7 Well 11I
type: Collection
persistent identity: iGEM 2018 Plate7 Well11I
display id: iGEM_2018_Plate7_Well11I
version: 1
owned by: myers
member: BBa_K1758331

iGEM 2018 Distribution Plate 7 Well 10E
type: Collection
persistent identity: iGEM 2018 Plate7 Well10E
display id: iGEM_2018_Plate7_Well10E
version: 1
owned by: myers
member: BBa_K1696003

iGEM 2018 Distribution Plate 7 Well 1A
type: Collection
persistent identity: iGEM 2018 Plate7 Well1A
display id: iGEM_2018_Plate7_Well1A
version: 1
owned by: myers
member: BBa_K1763000

iGEM 2018 Distribution Plate 7 Well 19I
type: Collection
persistent identity: iGEM 2018 Plate7 Well19I
display id: iGEM_2018_Plate7_Well19I
version: 1
owned by: myers
member: BBa_K1789001

iGEM 2018 Distribution Plate 7 Well 2I
type: Collection
persistent identity: iGEM 2018 Plate7 Well2I
display id: iGEM_2018_Plate7_Well2I
version: 1
owned by: myers
member: BBa_K1674002

iGEM 2018 Distribution Plate 7 Well 17L
type: Collection
persistent identity: iGEM 2018 Plate7 Well17L
display id: iGEM_2018_Plate7_Well17L
version: 1
owned by: myers
member: BBa_K1666002

iGEM 2018 Distribution Plate 7 Well 6I
type: Collection
persistent identity: iGEM 2018 Plate7 Well6I
display id: iGEM_2018_Plate7_Well6I
version: 1
owned by: myers
member: BBa_K1763439

iGEM 2018 Distribution Plate 7 Well 7J
type: Collection
persistent identity: iGEM 2018 Plate7 Well7J
display id: iGEM_2018_Plate7_Well7J
version: 1
owned by: myers
member: BBa_K1847012

iGEM 2018 Distribution Plate 7 Well 9C
type: Collection
persistent identity: iGEM 2018 Plate7 Well9C
display id: iGEM_2018_Plate7_Well9C
version: 1
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member: BBa_K1758203

iGEM 2018 Distribution Plate 7 Well 5D
type: Collection
persistent identity: iGEM 2018 Plate7 Well5D
display id: iGEM_2018_Plate7_Well5D
version: 1
owned by: myers
member: BBa_K1690005

iGEM 2018 Distribution Plate 7 Well 21B
type: Collection
persistent identity: iGEM 2018 Plate7 Well21B
display id: iGEM_2018_Plate7_Well21B
version: 1
owned by: myers
member: BBa_I20270

iGEM 2018 Distribution Plate 7 Well 6G
type: Collection
persistent identity: iGEM 2018 Plate7 Well6G
display id: iGEM_2018_Plate7_Well6G
version: 1
owned by: myers
member: BBa_K1763438

iGEM 2018 Distribution Plate 7 Well 22M
type: Collection
persistent identity: iGEM 2018 Plate7 Well22M
display id: iGEM_2018_Plate7_Well22M
version: 1
owned by: myers
member: BBa_K1689013

iGEM 2018 Distribution Plate 7 Well 13G
type: Collection
persistent identity: iGEM 2018 Plate7 Well13G
display id: iGEM_2018_Plate7_Well13G
version: 1
owned by: myers
member: BBa_K1758375

iGEM 2018 Distribution Plate 7 Well 21F
type: Collection
persistent identity: iGEM 2018 Plate7 Well21F
display id: iGEM_2018_Plate7_Well21F
version: 1
owned by: myers
member: https://synbiohub.org/public/igem/BBa_J364000/1

iGEM 2018 Distribution Plate 7 Well 17E
type: Collection
persistent identity: iGEM 2018 Plate7 Well17E
display id: iGEM_2018_Plate7_Well17E
version: 1
owned by: myers
member: BBa_K1598001

iGEM 2018 Distribution Plate 7 Well 1C
type: Collection
persistent identity: iGEM 2018 Plate7 Well1C
display id: iGEM_2018_Plate7_Well1C
version: 1
owned by: myers
member: BBa_K1763001

iGEM 2018 Distribution Plate 7 Well 5H
type: Collection
persistent identity: iGEM 2018 Plate7 Well5H
display id: iGEM_2018_Plate7_Well5H
version: 1
owned by: myers
member: BBa_K1583204

iGEM 2018 Distribution Plate 7 Well 4H
type: Collection
persistent identity: iGEM 2018 Plate7 Well4H
display id: iGEM_2018_Plate7_Well4H
version: 1
owned by: myers
member: https://synbiohub.org/public/igem/BBa_J364001/1

iGEM 2018 Distribution Plate 7 Well 11O
type: Collection
persistent identity: iGEM 2018 Plate7 Well11O
display id: iGEM_2018_Plate7_Well11O
version: 1
owned by: myers
member: BBa_K1758342

iGEM 2018 Distribution Plate 7 Well 11G
type: Collection
persistent identity: iGEM 2018 Plate7 Well11G
display id: iGEM_2018_Plate7_Well11G
version: 1
owned by: myers
member: BBa_K1758330

iGEM 2018 Distribution Plate 7 Well 9P
type: Collection
persistent identity: iGEM 2018 Plate7 Well9P
display id: iGEM_2018_Plate7_Well9P
version: 1
owned by: myers
member: BBa_K1799010

iGEM 2018 Distribution Plate 7 Well 17H
type: Collection
persistent identity: iGEM 2018 Plate7 Well17H
display id: iGEM_2018_Plate7_Well17H
version: 1
owned by: myers
member: BBa_K1666000

iGEM 2018 Distribution Plate 7 Well 8O
type: Collection
persistent identity: iGEM 2018 Plate7 Well8O
display id: iGEM_2018_Plate7_Well8O
version: 1
owned by: myers
member: BBa_K1850013

iGEM 2018 Distribution Plate 7 Well 21C
type: Collection
persistent identity: iGEM 2018 Plate7 Well21C
display id: iGEM_2018_Plate7_Well21C
version: 1
owned by: myers
member: BBa_K1789008

iGEM 2018 Distribution Plate 7 Well 21H
type: Collection
persistent identity: iGEM 2018 Plate7 Well21H
display id: iGEM_2018_Plate7_Well21H
version: 1
owned by: myers
member: https://synbiohub.org/public/igem/BBa_J364001/1

iGEM 2018 Distribution Plate 7 Well 4F
type: Collection
persistent identity: iGEM 2018 Plate7 Well4F
display id: iGEM_2018_Plate7_Well4F
version: 1
owned by: myers
member: https://synbiohub.org/public/igem/BBa_J364000/1

iGEM 2018 Distribution Plate 7 Well 19K
type: Collection
persistent identity: iGEM 2018 Plate7 Well19K
display id: iGEM_2018_Plate7_Well19K
version: 1
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member: BBa_K1789002

iGEM 2018 Distribution Plate 7 Well 2K
type: Collection
persistent identity: iGEM 2018 Plate7 Well2K
display id: iGEM_2018_Plate7_Well2K
version: 1
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member: BBa_K1674003

iGEM 2018 Distribution Plate 7 Well 22O
type: Collection
persistent identity: iGEM 2018 Plate7 Well22O
display id: iGEM_2018_Plate7_Well22O
version: 1
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member: BBa_K1689014

iGEM 2018 Distribution Plate 7 Well 20M
type: Collection
persistent identity: iGEM 2018 Plate7 Well20M
display id: iGEM_2018_Plate7_Well20M
version: 1
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member: BBa_K1825005

iGEM 2018 Distribution Plate 7 Well 9E
type: Collection
persistent identity: iGEM 2018 Plate7 Well9E
display id: iGEM_2018_Plate7_Well9E
version: 1
owned by: myers
member: BBa_K1758204

iGEM 2018 Distribution Plate 7 Well 12A
type: Collection
persistent identity: iGEM 2018 Plate7 Well12A
display id: iGEM_2018_Plate7_Well12A
version: 1
owned by: myers
member: BBa_K1640008

iGEM 2018 Distribution Plate 7 Well 14O
type: Collection
persistent identity: iGEM 2018 Plate7 Well14O
display id: iGEM_2018_Plate7_Well14O
version: 1
owned by: myers
member: BBa_K1602013

iGEM 2018 Distribution Plate 7 Well 13L
type: Collection
persistent identity: iGEM 2018 Plate7 Well13L
display id: iGEM_2018_Plate7_Well13L
version: 1
owned by: myers
member: BBa_K1795013

iGEM 2018 Distribution Plate 7 Well 23A
type: Collection
persistent identity: iGEM 2018 Plate7 Well23A
display id: iGEM_2018_Plate7_Well23A
version: 1
owned by: myers
member: BBa_K1632005

iGEM 2018 Distribution Plate 7 Well 1H
type: Collection
persistent identity: iGEM 2018 Plate7 Well1H
display id: iGEM_2018_Plate7_Well1H
version: 1
owned by: myers
member: Si-tag1

iGEM 2018 Distribution Plate 7 Well 17C
type: Collection
persistent identity: iGEM 2018 Plate7 Well17C
display id: iGEM_2018_Plate7_Well17C
version: 1
owned by: myers
member: DsbDspB

iGEM 2018 Distribution Plate 7 Well 9G
type: Collection
persistent identity: iGEM 2018 Plate7 Well9G
display id: iGEM_2018_Plate7_Well9G
version: 1
owned by: myers
member: BBa_K1758300

iGEM 2018 Distribution Plate 7 Well 16M
type: Collection
persistent identity: iGEM 2018 Plate7 Well16M
display id: iGEM_2018_Plate7_Well16M
version: 1
owned by: myers
member: RBS-His-Sc

iGEM 2018 Distribution Plate 7 Well 15N
type: Collection
persistent identity: iGEM 2018 Plate7 Well15N
display id: iGEM_2018_Plate7_Well15N
version: 1
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member: BBa_K1682018

iGEM 2018 Distribution Plate 7 Well 1M
type: Collection
persistent identity: iGEM 2018 Plate7 Well1M
display id: iGEM_2018_Plate7_Well1M
version: 1
owned by: myers
member: BBa_K1763012

iGEM 2018 Distribution Plate 7 Well 3G
type: Collection
persistent identity: iGEM 2018 Plate7 Well3G
display id: iGEM_2018_Plate7_Well3G
version: 1
owned by: myers
member: BBa_K1692006

iGEM 2018 Distribution Plate 7 Well 14C
type: Collection
persistent identity: iGEM 2018 Plate7 Well14C
display id: iGEM_2018_Plate7_Well14C
version: 1
owned by: myers
member: GRE3

iGEM 2018 Distribution Plate 7 Well 5B
type: Collection
persistent identity: iGEM 2018 Plate7 Well5B
display id: iGEM_2018_Plate7_Well5B
version: 1
owned by: myers
member: BBa_K1690004

iGEM 2018 Distribution Plate 7 Well 22C
type: Collection
persistent identity: iGEM 2018 Plate7 Well22C
display id: iGEM_2018_Plate7_Well22C
version: 1
owned by: myers
member: BBa_K1689002

iGEM 2018 Distribution Plate 7 Well 9I
type: Collection
persistent identity: iGEM 2018 Plate7 Well9I
display id: iGEM_2018_Plate7_Well9I
version: 1
owned by: myers
member: BBa_K1758301

iGEM 2018 Distribution Plate 7 Well 19M
type: Collection
persistent identity: iGEM 2018 Plate7 Well19M
display id: iGEM_2018_Plate7_Well19M
version: 1
owned by: myers
member: BBa_K1789003

iGEM 2018 Distribution Plate 7 Well 24K
type: Collection
persistent identity: iGEM 2018 Plate7 Well24K
display id: iGEM_2018_Plate7_Well24K
version: 1
owned by: myers
member: Mcfp3

iGEM 2018 Distribution Plate 7 Well 6A
type: Collection
persistent identity: iGEM 2018 Plate7 Well6A
display id: iGEM_2018_Plate7_Well6A
version: 1
owned by: myers
member: BBa_K1763427

iGEM 2018 Distribution Plate 7 Well 11C
type: Collection
persistent identity: iGEM 2018 Plate7 Well11C
display id: iGEM_2018_Plate7_Well11C
version: 1
owned by: myers
member: BBa_K1758323

iGEM 2018 Distribution Plate 7 Well 23C
type: Collection
persistent identity: iGEM 2018 Plate7 Well23C
display id: iGEM_2018_Plate7_Well23C
version: 1
owned by: myers
member: BBa_K1632006

iGEM 2018 Distribution Plate 7 Well 9J
type: Collection
persistent identity: iGEM 2018 Plate7 Well9J
display id: iGEM_2018_Plate7_Well9J
version: 1
owned by: myers
member: BBa_K1799006

iGEM 2018 Distribution Plate 7 Well 24O
type: Collection
persistent identity: iGEM 2018 Plate7 Well24O
display id: iGEM_2018_Plate7_Well24O
version: 1
owned by: myers
member: BBa_J04450

iGEM 2018 Distribution Plate 7 Well 19O
type: Collection
persistent identity: iGEM 2018 Plate7 Well19O
display id: iGEM_2018_Plate7_Well19O
version: 1
owned by: myers
member: BBa_K1789004

iGEM 2018 Distribution Plate 7 Well 2M
type: Collection
persistent identity: iGEM 2018 Plate7 Well2M
display id: iGEM_2018_Plate7_Well2M
version: 1
owned by: myers
member: BBa_K1674004

iGEM 2018 Distribution Plate 7 Well 4J
type: Collection
persistent identity: iGEM 2018 Plate7 Well4J
display id: iGEM_2018_Plate7_Well4J
version: 1
owned by: myers
member: https://synbiohub.org/public/igem/BBa_J364002/1

iGEM 2018 Distribution Plate 7 Well 18A
type: Collection
persistent identity: iGEM 2018 Plate7 Well18A
display id: iGEM_2018_Plate7_Well18A
version: 1
owned by: myers
member: xynA-PDZli

iGEM 2018 Distribution Plate 7 Well 3E
type: Collection
persistent identity: iGEM 2018 Plate7 Well3E
display id: iGEM_2018_Plate7_Well3E
version: 1
owned by: myers
member: BBa_K1692005

iGEM 2018 Distribution Plate 7 Well 10I
type: Collection
persistent identity: iGEM 2018 Plate7 Well10I
display id: iGEM_2018_Plate7_Well10I
version: 1
owned by: myers
member: BBa_K1696009

iGEM 2018 Distribution Plate 7 Well 1K
type: Collection
persistent identity: iGEM 2018 Plate7 Well1K
display id: iGEM_2018_Plate7_Well1K
version: 1
owned by: myers
member: BBa_K1763011

iGEM 2018 Distribution Plate 7 Well 15C
type: Collection
persistent identity: iGEM 2018 Plate7 Well15C
display id: iGEM_2018_Plate7_Well15C
version: 1
owned by: myers
member: DspB

iGEM 2018 Distribution Plate 7 Well 14A
type: Collection
persistent identity: iGEM 2018 Plate7 Well14A
display id: iGEM_2018_Plate7_Well14A
version: 1
owned by: myers
member: cadA

iGEM 2018 Distribution Plate 7 Well 17J
type: Collection
persistent identity: iGEM 2018 Plate7 Well17J
display id: iGEM_2018_Plate7_Well17J
version: 1
owned by: myers
member: BBa_K1666001

iGEM 2018 Distribution Plate 7 Well 8A
type: Collection
persistent identity: iGEM 2018 Plate7 Well8A
display id: iGEM_2018_Plate7_Well8A
version: 1
owned by: myers
member: BBa_K1763443

iGEM 2018 Distribution Plate 7 Well 5M
type: Collection
persistent identity: iGEM 2018 Plate7 Well5M
display id: iGEM_2018_Plate7_Well5M
version: 1
owned by: myers
member: BBa_K1585312

iGEM 2018 Distribution Plate 7 Well 17D
type: Collection
persistent identity: iGEM 2018 Plate7 Well17D
display id: iGEM_2018_Plate7_Well17D
version: 1
owned by: myers
member: BBa_K1829005

iGEM 2018 Distribution Plate 7 Well 21D
type: Collection
persistent identity: iGEM 2018 Plate7 Well21D
display id: iGEM_2018_Plate7_Well21D
version: 1
owned by: myers
member: p(tetR)

iGEM 2018 Distribution Plate 7 Well 8G
type: Collection
persistent identity: iGEM 2018 Plate7 Well8G
display id: iGEM_2018_Plate7_Well8G
version: 1
owned by: myers
member: BBa_K1763421

iGEM 2018 Distribution Plate 7 Well 18M
type: Collection
persistent identity: iGEM 2018 Plate7 Well18M
display id: iGEM_2018_Plate7_Well18M
version: 1
owned by: myers
member: BBa_K1602051

iGEM 2018 Distribution Plate 7 Well 13B
type: Collection
persistent identity: iGEM 2018 Plate7 Well13B
display id: iGEM_2018_Plate7_Well13B
version: 1
owned by: myers
member: BBa_K1795002

BBa_K1666000
type: ComponentDefinition
persistent identity: BBa K1666000
display id: BBa_K1666000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LsrA of LuxS/AI-2 signaling pathway in Salmonalla
creator: Heming Wang
created: 2015-08-25T11:00:00Z
modified: 2015-08-30T07:46:12Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2084_
m user id: 28478
owner id: 28478
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: In a process called quorum sensing, bacteria communicate with one another using secreted chemical signaling molecules termed autoinducers. As the density of the bacterial population increases, so does the concentration of secreted autoinducer molecules. When the concentration of the autoinducer reaches a critical threshold, it diffuses back into the cell and activates or represses certain target genes. An autoinducer called AI-2 is generated by many species of Gram-negative and Gram-positive bacteria. In the LuxS/AI-2 signaling system of Salmonella enterica serovar Typhimurium, AI-2 response involves genes named lsr (LuxS regulated) that encode ATP binding cassette-type transporter. And LsrA is ATP-binding protein of the ABC transporter. In our project, we set this part under a nisA promoter for the final purpose of constructing an integrated AI-2 response pathway in our engineering bacteria.
mutable notes: The lsrA gene was isolated from the genome of Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 using PCR.We added restriction enzyme sites when designing primers,and the assembly is done through the use of restriction sites (cutting and ligating).
mutable provenance: It comes from the genome of Salmonella enterica subsp. enterica serovar Typhimurium str. LT2(Gene ID: 1255601)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: LsrA
sequence: https://synbiohub.org/public/igem/BBa_K1666000_sequence/1

LsrA
type: SequenceAnnotation
persistent identity: annotation2439289
display id: annotation2439289
version: 1
top level: BBa_K1666000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1666000/annotation2439289/range2439289/1

https://synbiohub.org/public/igem/BBa_K1666000/annotation2439289/range2439289/1
type: Range
persistent identity: range2439289
display id: range2439289
version: 1
direction: direction/forward
top level: BBa_K1666000
start: 1
end: 1536
orientation: inline

BBa_K1614019
type: ComponentDefinition
persistent identity: BBa K1614019
display id: BBa_K1614019
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ATP Aptamer JAWS1 Spinach 2.1
creator: Daniel Heid
created: 2015-09-17T11:00:00Z
modified: 2015-09-20T03:17:44Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2031_
m user id: 24664
owner id: 24635
owning group id: 9
status: status/Planning
bookmark: false
mutable description: ATP Spinach 12
mutable provenance: Synthetic
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence annotation: ATP Spinach
sequence annotation: ATP Aptamer
sequence: https://synbiohub.org/public/igem/BBa_K1614019_sequence/1

ATP Spinach
type: SequenceAnnotation
persistent identity: annotation2476189
display id: annotation2476189
version: 1
top level: BBa_K1614019
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1614019/annotation2476189/range2476189/1

https://synbiohub.org/public/igem/BBa_K1614019/annotation2476189/range2476189/1
type: Range
persistent identity: range2476189
display id: range2476189
version: 1
direction: direction/forward
top level: BBa_K1614019
start: 1
end: 146
orientation: inline

ATP Aptamer
type: SequenceAnnotation
persistent identity: annotation2476188
display id: annotation2476188
version: 1
top level: BBa_K1614019
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1614019/annotation2476188/range2476188/1

https://synbiohub.org/public/igem/BBa_K1614019/annotation2476188/range2476188/1
type: Range
persistent identity: range2476188
display id: range2476188
version: 1
direction: direction/forward
top level: BBa_K1614019
start: 45
end: 101
orientation: inline

BBa_K1763427
type: ComponentDefinition
persistent identity: BBa K1763427
display id: BBa_K1763427
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: MaSp2-6(T7)
creator: Vinson Lam
created: 2015-09-14T11:00:00Z
modified: 2015-09-15T08:41:53Z
discontinued: false
dominant: false
group u list: _2185_
m user id: 27136
owner id: 27136
owning group id: 9
status: status/Planning
bookmark: false
mutable description: M2-6(1C3) under control of T7 promoter RBS.
mutable notes: no
mutable provenance: N. clavipes
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K525998
component: BBa_K1763426
sequence annotation: BBa_K1763426
sequence annotation: BBa_K525998
sequence: https://synbiohub.org/public/igem/BBa_K1763427_sequence/1

BBa_K525998
type: Component
persistent identity: component2456837
display id: component2456837
version: 1
top level: BBa_K1763427
access: public
definition: https://synbiohub.org/public/igem/BBa_K525998/1

BBa_K1763426
type: Component
persistent identity: component2456838
display id: component2456838
version: 1
top level: BBa_K1763427
access: public
definition: BBa_K1763426

BBa_K1763426
type: SequenceAnnotation
persistent identity: annotation2456838
display id: annotation2456838
version: 1
top level: BBa_K1763427
location: https://synbiohub.org/public/igem/BBa_K1763427/annotation2456838/range2456838/1
component: BBa_K1763426

https://synbiohub.org/public/igem/BBa_K1763427/annotation2456838/range2456838/1
type: Range
persistent identity: range2456838
display id: range2456838
version: 1
direction: direction/reverse
top level: BBa_K1763427
start: 39
end: 701
orientation: inline

BBa_K525998
type: SequenceAnnotation
persistent identity: annotation2456837
display id: annotation2456837
version: 1
top level: BBa_K1763427
location: https://synbiohub.org/public/igem/BBa_K1763427/annotation2456837/range2456837/1
component: BBa_K525998

https://synbiohub.org/public/igem/BBa_K1763427/annotation2456837/range2456837/1
type: Range
persistent identity: range2456837
display id: range2456837
version: 1
direction: direction/reverse
top level: BBa_K1763427
start: 1
end: 32
orientation: inline

BBa_K1763442
type: ComponentDefinition
persistent identity: BBa K1763442
display id: BBa_K1763442
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: MaSp1/2[1:2]-12(1C3)
creator: Vinson Lam
created: 2015-09-14T11:00:00Z
modified: 2015-09-17T12:06:58Z
discontinued: false
dominant: false
group u list: _2185_
m user id: 27136
owner id: 27136
owning group id: 9
status: status/Planning
bookmark: false
mutable description: MaSp1 and MaSp2 12-mer block co-polymer in pSB1C3, with no regulatory elements. Ratio of 1 MaSp1 to 2 MaSp2.
mutable notes: no
mutable provenance: N. clavipes
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: M1-CA
sequence annotation: M1-AB
sequence annotation: M2-CA
sequence annotation: M2-BC
sequence annotation: M1-BC
sequence annotation: 8-his tag
sequence annotation: M2-AB
sequence: https://synbiohub.org/public/igem/BBa_K1763442_sequence/1

M1-CA
type: SequenceAnnotation
persistent identity: annotation2464153
display id: annotation2464153
version: 1
top level: BBa_K1763442
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763442/annotation2464153/range2464153/1

https://synbiohub.org/public/igem/BBa_K1763442/annotation2464153/range2464153/1
type: Range
persistent identity: range2464153
display id: range2464153
version: 1
direction: direction/forward
top level: BBa_K1763442
start: 240
end: 341
orientation: inline

M1-AB
type: SequenceAnnotation
persistent identity: annotation2464155
display id: annotation2464155
version: 1
top level: BBa_K1763442
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763442/annotation2464155/range2464155/1

https://synbiohub.org/public/igem/BBa_K1763442/annotation2464155/range2464155/1
type: Range
persistent identity: range2464155
display id: range2464155
version: 1
direction: direction/forward
top level: BBa_K1763442
start: 36
end: 137
orientation: inline

M2-CA
type: SequenceAnnotation
persistent identity: annotation2464146
display id: annotation2464146
version: 1
top level: BBa_K1763442
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763442/annotation2464146/range2464146/1

https://synbiohub.org/public/igem/BBa_K1763442/annotation2464146/range2464146/1
type: Range
persistent identity: range2464146
display id: range2464146
version: 1
direction: direction/forward
top level: BBa_K1763442
start: 1158
end: 1259
orientation: inline

M2-BC
type: SequenceAnnotation
persistent identity: annotation2464147
display id: annotation2464147
version: 1
top level: BBa_K1763442
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763442/annotation2464147/range2464147/1

https://synbiohub.org/public/igem/BBa_K1763442/annotation2464147/range2464147/1
type: Range
persistent identity: range2464147
display id: range2464147
version: 1
direction: direction/forward
top level: BBa_K1763442
start: 1056
end: 1157
orientation: inline

M1-BC
type: SequenceAnnotation
persistent identity: annotation2464149
display id: annotation2464149
version: 1
top level: BBa_K1763442
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763442/annotation2464149/range2464149/1

https://synbiohub.org/public/igem/BBa_K1763442/annotation2464149/range2464149/1
type: Range
persistent identity: range2464149
display id: range2464149
version: 1
direction: direction/forward
top level: BBa_K1763442
start: 138
end: 239
orientation: inline

M2-BC
type: SequenceAnnotation
persistent identity: annotation2464144
display id: annotation2464144
version: 1
top level: BBa_K1763442
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763442/annotation2464144/range2464144/1

https://synbiohub.org/public/igem/BBa_K1763442/annotation2464144/range2464144/1
type: Range
persistent identity: range2464144
display id: range2464144
version: 1
direction: direction/forward
top level: BBa_K1763442
start: 444
end: 545
orientation: inline

M1-AB
type: SequenceAnnotation
persistent identity: annotation2464152
display id: annotation2464152
version: 1
top level: BBa_K1763442
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763442/annotation2464152/range2464152/1

https://synbiohub.org/public/igem/BBa_K1763442/annotation2464152/range2464152/1
type: Range
persistent identity: range2464152
display id: range2464152
version: 1
direction: direction/forward
top level: BBa_K1763442
start: 342
end: 443
orientation: inline

M2-CA
type: SequenceAnnotation
persistent identity: annotation2464145
display id: annotation2464145
version: 1
top level: BBa_K1763442
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763442/annotation2464145/range2464145/1

https://synbiohub.org/public/igem/BBa_K1763442/annotation2464145/range2464145/1
type: Range
persistent identity: range2464145
display id: range2464145
version: 1
direction: direction/forward
top level: BBa_K1763442
start: 852
end: 953
orientation: inline

M2-BC
type: SequenceAnnotation
persistent identity: annotation2464150
display id: annotation2464150
version: 1
top level: BBa_K1763442
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763442/annotation2464150/range2464150/1

https://synbiohub.org/public/igem/BBa_K1763442/annotation2464150/range2464150/1
type: Range
persistent identity: range2464150
display id: range2464150
version: 1
direction: direction/forward
top level: BBa_K1763442
start: 750
end: 851
orientation: inline

8-his tag
type: SequenceAnnotation
persistent identity: annotation2464143
display id: annotation2464143
version: 1
top level: BBa_K1763442
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1763442/annotation2464143/range2464143/1

https://synbiohub.org/public/igem/BBa_K1763442/annotation2464143/range2464143/1
type: Range
persistent identity: range2464143
display id: range2464143
version: 1
direction: direction/forward
top level: BBa_K1763442
start: 4
end: 27
orientation: inline

M2-AB
type: SequenceAnnotation
persistent identity: annotation2464151
display id: annotation2464151
version: 1
top level: BBa_K1763442
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763442/annotation2464151/range2464151/1

https://synbiohub.org/public/igem/BBa_K1763442/annotation2464151/range2464151/1
type: Range
persistent identity: range2464151
display id: range2464151
version: 1
direction: direction/forward
top level: BBa_K1763442
start: 648
end: 749
orientation: inline

M2-AB
type: SequenceAnnotation
persistent identity: annotation2464148
display id: annotation2464148
version: 1
top level: BBa_K1763442
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763442/annotation2464148/range2464148/1

https://synbiohub.org/public/igem/BBa_K1763442/annotation2464148/range2464148/1
type: Range
persistent identity: range2464148
display id: range2464148
version: 1
direction: direction/forward
top level: BBa_K1763442
start: 954
end: 1055
orientation: inline

M2-CA
type: SequenceAnnotation
persistent identity: annotation2464154
display id: annotation2464154
version: 1
top level: BBa_K1763442
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763442/annotation2464154/range2464154/1

https://synbiohub.org/public/igem/BBa_K1763442/annotation2464154/range2464154/1
type: Range
persistent identity: range2464154
display id: range2464154
version: 1
direction: direction/forward
top level: BBa_K1763442
start: 546
end: 647
orientation: inline

BBa_K1689015
type: ComponentDefinition
persistent identity: BBa K1689015
display id: BBa_K1689015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: F[1,2]-dCas9
creator: Wei weijia
created: 2015-09-15T11:00:00Z
modified: 2015-09-27T01:33:33Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2107_
m user id: 26531
owner id: 26531
owning group id: 9
status: status/Planning
bookmark: false
mutable description: pSB1C3-6His-F[1,2]-dCas9, segment F[1,2] fusion with dCas9, 6His tag allows for protein purification.
mutable notes: the GC% is high of F[1,2]
mutable provenance: E.coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: His
sequence annotation: random spacer
sequence annotation: F[1,2]
sequence annotation: dCas9
sequence annotation: stop
sequence annotation: F[1,2]-dCas9
sequence annotation: GGGGS*2 Linker
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_K1689015_sequence/1

His
type: SequenceAnnotation
persistent identity: annotation2469122
display id: annotation2469122
version: 1
top level: BBa_K1689015
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1689015/annotation2469122/range2469122/1

https://synbiohub.org/public/igem/BBa_K1689015/annotation2469122/range2469122/1
type: Range
persistent identity: range2469122
display id: range2469122
version: 1
direction: direction/forward
top level: BBa_K1689015
start: 37
end: 54
orientation: inline

random spacer
type: SequenceAnnotation
persistent identity: annotation2477781
display id: annotation2477781
version: 1
top level: BBa_K1689015
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1689015/annotation2477781/range2477781/1

https://synbiohub.org/public/igem/BBa_K1689015/annotation2477781/range2477781/1
type: Range
persistent identity: range2477781
display id: range2477781
version: 1
direction: direction/forward
top level: BBa_K1689015
start: 1
end: 33
orientation: inline

F[1,2]
type: SequenceAnnotation
persistent identity: annotation2469133
display id: annotation2469133
version: 1
top level: BBa_K1689015
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1689015/annotation2469133/range2469133/1

https://synbiohub.org/public/igem/BBa_K1689015/annotation2469133/range2469133/1
type: Range
persistent identity: range2469133
display id: range2469133
version: 1
direction: direction/forward
top level: BBa_K1689015
start: 55
end: 315
orientation: inline

dCas9
type: SequenceAnnotation
persistent identity: annotation2469157
display id: annotation2469157
version: 1
top level: BBa_K1689015
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1689015/annotation2469157/range2469157/1

https://synbiohub.org/public/igem/BBa_K1689015/annotation2469157/range2469157/1
type: Range
persistent identity: range2469157
display id: range2469157
version: 1
direction: direction/forward
top level: BBa_K1689015
start: 346
end: 4449
orientation: inline

random spacer
type: SequenceAnnotation
persistent identity: annotation2477784
display id: annotation2477784
version: 1
top level: BBa_K1689015
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1689015/annotation2477784/range2477784/1

https://synbiohub.org/public/igem/BBa_K1689015/annotation2477784/range2477784/1
type: Range
persistent identity: range2477784
display id: range2477784
version: 1
direction: direction/forward
top level: BBa_K1689015
start: 4453
end: 4520
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2477783
display id: annotation2477783
version: 1
top level: BBa_K1689015
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1689015/annotation2477783/range2477783/1

https://synbiohub.org/public/igem/BBa_K1689015/annotation2477783/range2477783/1
type: Range
persistent identity: range2477783
display id: range2477783
version: 1
direction: direction/forward
top level: BBa_K1689015
start: 4450
end: 4452
orientation: inline

F[1,2]-dCas9
type: SequenceAnnotation
persistent identity: annotation2470142
display id: annotation2470142
version: 1
top level: BBa_K1689015
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1689015/annotation2470142/range2470142/1

https://synbiohub.org/public/igem/BBa_K1689015/annotation2470142/range2470142/1
type: Range
persistent identity: range2470142
display id: range2470142
version: 1
direction: direction/forward
top level: BBa_K1689015
start: 34
end: 4452
orientation: inline

GGGGS*2 Linker
type: SequenceAnnotation
persistent identity: annotation2470140
display id: annotation2470140
version: 1
top level: BBa_K1689015
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1689015/annotation2470140/range2470140/1

https://synbiohub.org/public/igem/BBa_K1689015/annotation2470140/range2470140/1
type: Range
persistent identity: range2470140
display id: range2470140
version: 1
direction: direction/forward
top level: BBa_K1689015
start: 316
end: 345
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2477782
display id: annotation2477782
version: 1
top level: BBa_K1689015
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1689015/annotation2477782/range2477782/1

https://synbiohub.org/public/igem/BBa_K1689015/annotation2477782/range2477782/1
type: Range
persistent identity: range2477782
display id: range2477782
version: 1
direction: direction/forward
top level: BBa_K1689015
start: 34
end: 36
orientation: inline

ldhE-Lc
type: ComponentDefinition
persistent identity: BBa K1696001
display id: BBa_K1696001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: L-Lactate producing module from Lactobacillus casei
creator: Yue Liu
created: 2015-09-10T11:00:00Z
modified: 2015-09-11T10:20:43Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2114_
m user id: 16881
owner id: 16881
owning group id: 9
status: status/Planning
bookmark: false
mutable description: The ldh gene can produce L-lactate from pyruvate, which not only increase the overall amounts of lactate but reduce the byproduct generation.
mutable notes: coden optimization
mutable provenance: From Lactobacillus casei
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: ldhA
sequence: https://synbiohub.org/public/igem/BBa_K1696001_sequence/1

ldhA
type: SequenceAnnotation
persistent identity: annotation2449232
display id: annotation2449232
version: 1
top level: ldhE-Lc
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1696001/annotation2449232/range2449232/1

https://synbiohub.org/public/igem/BBa_K1696001/annotation2449232/range2449232/1
type: Range
persistent identity: range2449232
display id: range2449232
version: 1
direction: direction/forward
top level: ldhE-Lc
start: 1
end: 981
orientation: inline

BBa_K1666003
type: ComponentDefinition
persistent identity: BBa K1666003
display id: BBa_K1666003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LsrD of LuxS/AI-2 signaling pathway in Salmonalla
creator: Heming Wang
created: 2015-09-05T11:00:00Z
modified: 2015-09-06T08:08:52Z
discontinued: false
dominant: false
group u list: _2084_
m user id: 23948
owner id: 23948
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Quorum sensing is a process of bacterial cell-to-cell communication involving the production and detection of extracellular signaling molecules called autoinducers. And autoinducer-2 (AI-2) has been proposed to serve as a 'universal signal' for interspecies communication. In the LuxS/AI-2 signaling system of Salmonella Typhimurium, AI-2 response involves ATP binding cassette transporter encoded by genes named Lsr (LuxS regulated). And LsrD is thetransmembrane protein which constructed the ABC transport. In our project, we set this protein-coding part under a nisA promoter and try to integrate them in the genome of Lactobacillus or Lactococcus for the final purpose of constructing an integrated AI-2 response pathway of Salmonella in the engineered bacteria.
mutable notes: The lsrD gene was isolated from the genome of Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 using PCR. We added restriction enzyme sites when designing primers, and the assembly is done through the use of restriction sites (cutting and ligating).
mutable provenance: We extracted the genomic DNA of Salmonella enterica subsp. enterica serovar Typhimurium str. LT2, and used PCR to isolate the gene LsrA(Gene ID: 1255603).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: lsrD
sequence: https://synbiohub.org/public/igem/BBa_K1666003_sequence/1

lsrD
type: SequenceAnnotation
persistent identity: annotation2445115
display id: annotation2445115
version: 1
top level: BBa_K1666003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1666003/annotation2445115/range2445115/1

https://synbiohub.org/public/igem/BBa_K1666003/annotation2445115/range2445115/1
type: Range
persistent identity: range2445115
display id: range2445115
version: 1
direction: direction/forward
top level: BBa_K1666003
start: 1
end: 1002
orientation: inline

BBa_K1602012
type: ComponentDefinition
persistent identity: BBa K1602012
display id: BBa_K1602012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: yjhG
creator: Leon Kraus, Jonathan Pletzer-Zelgert, Jonas Sindlinger, Carmen Sakas Gandullo, Sebastian Holzner, Elena Tonchevska, Mirjam Haun
created: 2015-09-13T11:00:00Z
modified: 2015-09-18T06:39:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2019_
m user id: 26220
owner id: 25878
owning group id: 9
status: status/Planning
bookmark: false
mutable description: BLANK
mutable notes: BLANK
mutable provenance: BLANK
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1602012_sequence/1

BBa_K1829007
type: ComponentDefinition
persistent identity: BBa K1829007
display id: BBa_K1829007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ste2: ligand receptor of mating factor alpha
creator: Hunter Collins
created: 2015-09-17T11:00:00Z
modified: 2015-09-18T01:22:24Z
discontinued: false
dominant: false
group u list: _2255_
m user id: 27847
owner id: 27847
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Ste2 is an integral ligand receptor of alpha factor found endogenously in MATa S. Cerevisiae (budding yeast).
mutable notes: Nucleotide 1104 changed from T to C to remove PstI cutsite; silent mutation
mutable provenance: Ste2 is found in the genomic sequence of MATa S. Cerevisiae.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K1829007_sequence/1

BBa_K1690004
type: ComponentDefinition
persistent identity: BBa K1690004
display id: BBa_K1690004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pheromone receptor (C. albicans)
creator: Anna Sosnova, Pavel Zach
created: 2015-09-16T11:00:00Z
modified: 2015-09-17T05:34:43Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2108_
m user id: 25090
owner id: 25090
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This is a coding sequence of C. albicans mating pheromone receptor. Cells with this receptor being expressed react to the C. albicans (can be found in the registry as [http://parts.igem.org/Part:BBa_K1690001 BBa_K1690001]) mating pheromone by the activation of the pheromone mating pathway. The coding sequence was codon optimized for the expression in S. cerevisiae. Detailed description can be obtained from the [http://2015.igem.org/Team:Czech_Republic/Project/Synthetic_signals_and_receptors#Receptors iGEM 2015 Czech Republic wiki].
mutable notes: The coding sequence was codon optimized for the expression in S. cerevisiae. Kozak sequence was added before the start codon for a better translation.
mutable provenance: The whole sequence was synthetized.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: start
sequence annotation: Kozak
sequence annotation: stop
sequence annotation: Receptor
sequence: https://synbiohub.org/public/igem/BBa_K1690004_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation2464330
display id: annotation2464330
version: 1
top level: BBa_K1690004
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1690004/annotation2464330/range2464330/1

https://synbiohub.org/public/igem/BBa_K1690004/annotation2464330/range2464330/1
type: Range
persistent identity: range2464330
display id: range2464330
version: 1
direction: direction/forward
top level: BBa_K1690004
start: 7
end: 9
orientation: inline

Kozak
type: SequenceAnnotation
persistent identity: annotation2464329
display id: annotation2464329
version: 1
top level: BBa_K1690004
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1690004/annotation2464329/range2464329/1

https://synbiohub.org/public/igem/BBa_K1690004/annotation2464329/range2464329/1
type: Range
persistent identity: range2464329
display id: range2464329
version: 1
direction: direction/forward
top level: BBa_K1690004
start: 1
end: 6
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2464332
display id: annotation2464332
version: 1
top level: BBa_K1690004
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1690004/annotation2464332/range2464332/1

https://synbiohub.org/public/igem/BBa_K1690004/annotation2464332/range2464332/1
type: Range
persistent identity: range2464332
display id: range2464332
version: 1
direction: direction/forward
top level: BBa_K1690004
start: 1411
end: 1413
orientation: inline

Receptor
type: SequenceAnnotation
persistent identity: annotation2464331
display id: annotation2464331
version: 1
top level: BBa_K1690004
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1690004/annotation2464331/range2464331/1

https://synbiohub.org/public/igem/BBa_K1690004/annotation2464331/range2464331/1
type: Range
persistent identity: range2464331
display id: range2464331
version: 1
direction: direction/forward
top level: BBa_K1690004
start: 10
end: 1410
orientation: inline

BBa_K1614018
type: ComponentDefinition
persistent identity: BBa K1614018
display id: BBa_K1614018
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ATP Aptamer Spinach 2.1
creator: Daniel Heid
created: 2015-09-17T11:00:00Z
modified: 2015-09-18T05:43:54Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2031_
m user id: 25630
owner id: 24635
owning group id: 9
status: status/Planning
bookmark: false
mutable description: ATP Spinach
mutable provenance: Synthetic
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence: https://synbiohub.org/public/igem/BBa_K1614018_sequence/1

BBa_K1585212
type: ComponentDefinition
persistent identity: BBa K1585212
display id: BBa_K1585212
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0034.PHI
creator: Laura Grabowski
created: 2015-08-24T11:00:00Z
modified: 2015-08-28T08:24:03Z
discontinued: false
dominant: false
group u list: _2002_
m user id: 19522
owner id: 24505
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This is the translational unit of the 6-phospho-3-hexuloisomerase (PHI) from Bacillus methanolicus MGA3. It is codon optimized for E.coli.
mutable notes: RFC10 compatible
mutable provenance: Bacillus methanolicus
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0034
component: BBa_K1585202
sequence annotation: BBa_B0034
sequence annotation: BBa_K1585202
sequence: https://synbiohub.org/public/igem/BBa_K1585212_sequence/1

BBa_B0034
type: Component
persistent identity: component2439455
display id: component2439455
version: 1
top level: BBa_K1585212
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1585202
type: Component
persistent identity: component2439456
display id: component2439456
version: 1
top level: BBa_K1585212
access: public
definition: https://synbiohub.org/public/igem/BBa_K1585202/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2439455
display id: annotation2439455
version: 1
top level: BBa_K1585212
location: https://synbiohub.org/public/igem/BBa_K1585212/annotation2439455/range2439455/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1585212/annotation2439455/range2439455/1
type: Range
persistent identity: range2439455
display id: range2439455
version: 1
direction: direction/reverse
top level: BBa_K1585212
start: 1
end: 12
orientation: inline

BBa_K1585202
type: SequenceAnnotation
persistent identity: annotation2439456
display id: annotation2439456
version: 1
top level: BBa_K1585212
location: https://synbiohub.org/public/igem/BBa_K1585212/annotation2439456/range2439456/1
component: BBa_K1585202

https://synbiohub.org/public/igem/BBa_K1585212/annotation2439456/range2439456/1
type: Range
persistent identity: range2439456
display id: range2439456
version: 1
direction: direction/reverse
top level: BBa_K1585212
start: 19
end: 576
orientation: inline

BBa_K1689012
type: ComponentDefinition
persistent identity: BBa K1689012
display id: BBa_K1689012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: dCas9-Nlact
creator: Jiaofeng Li
created: 2015-09-15T11:00:00Z
modified: 2015-11-17T09:39:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2107_
m user id: 26531
owner id: 26434
owning group id: 9
status: status/Planning
bookmark: false
mutable description: NFluc of lactamase fused with dCas9, His tag allows for protein purification
mutable notes: goldengate
mutable provenance: E.coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: GGGGS*2 Linker
sequence annotation: dCas9
sequence annotation: random spacer
sequence annotation: His
sequence annotation: stop
sequence annotation: dCas9-Nlact
sequence annotation: Nlact for N terminal of β-Lactamase
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_K1689012_sequence/1

GGGGS*2 Linker
type: SequenceAnnotation
persistent identity: annotation2470090
display id: annotation2470090
version: 1
top level: BBa_K1689012
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1689012/annotation2470090/range2470090/1

https://synbiohub.org/public/igem/BBa_K1689012/annotation2470090/range2470090/1
type: Range
persistent identity: range2470090
display id: range2470090
version: 1
direction: direction/forward
top level: BBa_K1689012
start: 4156
end: 4185
orientation: inline

dCas9
type: SequenceAnnotation
persistent identity: annotation2470079
display id: annotation2470079
version: 1
top level: BBa_K1689012
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1689012/annotation2470079/range2470079/1

https://synbiohub.org/public/igem/BBa_K1689012/annotation2470079/range2470079/1
type: Range
persistent identity: range2470079
display id: range2470079
version: 1
direction: direction/forward
top level: BBa_K1689012
start: 55
end: 4155
orientation: inline

random spacer
type: SequenceAnnotation
persistent identity: annotation2477772
display id: annotation2477772
version: 1
top level: BBa_K1689012
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1689012/annotation2477772/range2477772/1

https://synbiohub.org/public/igem/BBa_K1689012/annotation2477772/range2477772/1
type: Range
persistent identity: range2477772
display id: range2477772
version: 1
direction: direction/forward
top level: BBa_K1689012
start: 4702
end: 4772
orientation: inline

His
type: SequenceAnnotation
persistent identity: annotation2470058
display id: annotation2470058
version: 1
top level: BBa_K1689012
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1689012/annotation2470058/range2470058/1

https://synbiohub.org/public/igem/BBa_K1689012/annotation2470058/range2470058/1
type: Range
persistent identity: range2470058
display id: range2470058
version: 1
direction: direction/forward
top level: BBa_K1689012
start: 37
end: 54
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2477771
display id: annotation2477771
version: 1
top level: BBa_K1689012
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1689012/annotation2477771/range2477771/1

https://synbiohub.org/public/igem/BBa_K1689012/annotation2477771/range2477771/1
type: Range
persistent identity: range2477771
display id: range2477771
version: 1
direction: direction/forward
top level: BBa_K1689012
start: 4699
end: 4701
orientation: inline

dCas9-Nlact
type: SequenceAnnotation
persistent identity: annotation2470073
display id: annotation2470073
version: 1
top level: BBa_K1689012
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1689012/annotation2470073/range2470073/1

https://synbiohub.org/public/igem/BBa_K1689012/annotation2470073/range2470073/1
type: Range
persistent identity: range2470073
display id: range2470073
version: 1
direction: direction/forward
top level: BBa_K1689012
start: 34
end: 4701
orientation: inline

random spacer
type: SequenceAnnotation
persistent identity: annotation2477770
display id: annotation2477770
version: 1
top level: BBa_K1689012
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1689012/annotation2477770/range2477770/1

https://synbiohub.org/public/igem/BBa_K1689012/annotation2477770/range2477770/1
type: Range
persistent identity: range2477770
display id: range2477770
version: 1
direction: direction/forward
top level: BBa_K1689012
start: 1
end: 33
orientation: inline

Nlact for N terminal of β-Lactamase
type: SequenceAnnotation
persistent identity: annotation2470104
display id: annotation2470104
version: 1
top level: BBa_K1689012
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1689012/annotation2470104/range2470104/1

https://synbiohub.org/public/igem/BBa_K1689012/annotation2470104/range2470104/1
type: Range
persistent identity: range2470104
display id: range2470104
version: 1
direction: direction/forward
top level: BBa_K1689012
start: 4186
end: 4698
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2477769
display id: annotation2477769
version: 1
top level: BBa_K1689012
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1689012/annotation2477769/range2477769/1

https://synbiohub.org/public/igem/BBa_K1689012/annotation2477769/range2477769/1
type: Range
persistent identity: range2477769
display id: range2477769
version: 1
direction: direction/forward
top level: BBa_K1689012
start: 34
end: 36
orientation: inline

Si-tag1
type: ComponentDefinition
persistent identity: BBa K1592007
display id: BBa_K1592007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LIP prepro + E. coli ribosomal protein L2 (1-60aa) + YLcwp3 Fusion
creator: Shuyan Tang
created: 2015-09-04T11:00:00Z
modified: 2015-09-18T09:26:29Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2009_
m user id: 20267
owner id: 20267
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: This is the cell display fusion protein of Yarrowia lipolytica, composed of LIP prepro, interest protein, and YLcwp3. LIP prepro is signal peptide used to secrete the interest protein out of the cell, and the YLcwp3 is the anchor domain binding the interest protein to the cell wall of yeast. We use this system to display silica-tag and test its binding characteristics. E.coli ribosomal protein L2 was found to bind tightly to silicon particles, which have surfaces that are oxidized to silica. This L2 silica-binding tag, called the ??????Si-tag,?????? can be used for one-step targeting of functional proteins on silica surfaces. The silica-binding domains of E. coli L2 was mapped to amino acids 1???60, 61-202 and 203???273. We respectively test the silica-binding characteristics of this three regions and their combinations. E. coli ribosomal protein L2 (1-60) is the amino acids 1???60 of E. coli ribosomal protein L2, we call it si-tag1.
mutable notes: In order to replace more convenient, we respectively added BamHI, SalI, NdeI between LIP2 prepro, E. coli ribosomal protein L2 (1-60), GS linker, YLcwp3.
mutable provenance: E. coli ribosomal protein L2 was synthesized by IDT. LIP2 prepro and YLcwp3 were cloning from the plasmid JMP62 of our lab.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: his-tag
sequence annotation: SalI
sequence annotation: BBa_K1592000
sequence annotation: BBa_K1592002
sequence annotation: YLcwp3
sequence annotation: LIP2 prepro
sequence annotation: Si-tag1
sequence annotation: GS linker
sequence annotation: NdeI
sequence annotation: BamHI
sequence: https://synbiohub.org/public/igem/BBa_K1592007_sequence/1

his-tag
type: SequenceAnnotation
persistent identity: annotation2447678
display id: annotation2447678
version: 1
top level: Si-tag1
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1592007/annotation2447678/range2447678/1

https://synbiohub.org/public/igem/BBa_K1592007/annotation2447678/range2447678/1
type: Range
persistent identity: range2447678
display id: range2447678
version: 1
direction: direction/forward
top level: Si-tag1
start: 106
end: 123
orientation: inline

SalI
type: SequenceAnnotation
persistent identity: annotation2444373
display id: annotation2444373
version: 1
top level: Si-tag1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1592007/annotation2444373/range2444373/1

https://synbiohub.org/public/igem/BBa_K1592007/annotation2444373/range2444373/1
type: Range
persistent identity: range2444373
display id: range2444373
version: 1
direction: direction/forward
top level: Si-tag1
start: 304
end: 309
orientation: inline

BBa_K1592000
type: SequenceAnnotation
persistent identity: annotation2444370
display id: annotation2444370
version: 1
top level: Si-tag1
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1592007/annotation2444370/range2444370/1

https://synbiohub.org/public/igem/BBa_K1592007/annotation2444370/range2444370/1
type: Range
persistent identity: range2444370
display id: range2444370
version: 1
direction: direction/forward
top level: Si-tag1
start: 1
end: 99
orientation: inline

BBa_K1592002
type: SequenceAnnotation
persistent identity: annotation2444390
display id: annotation2444390
version: 1
top level: Si-tag1
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1592007/annotation2444390/range2444390/1

https://synbiohub.org/public/igem/BBa_K1592007/annotation2444390/range2444390/1
type: Range
persistent identity: range2444390
display id: range2444390
version: 1
direction: direction/forward
top level: Si-tag1
start: 343
end: 708
orientation: inline

YLcwp3
type: SequenceAnnotation
persistent identity: annotation2444389
display id: annotation2444389
version: 1
top level: Si-tag1
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1592007/annotation2444389/range2444389/1

https://synbiohub.org/public/igem/BBa_K1592007/annotation2444389/range2444389/1
type: Range
persistent identity: range2444389
display id: range2444389
version: 1
direction: direction/forward
top level: Si-tag1
start: 343
end: 708
orientation: inline

LIP2 prepro
type: SequenceAnnotation
persistent identity: annotation2444391
display id: annotation2444391
version: 1
top level: Si-tag1
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1592007/annotation2444391/range2444391/1

https://synbiohub.org/public/igem/BBa_K1592007/annotation2444391/range2444391/1
type: Range
persistent identity: range2444391
display id: range2444391
version: 1
direction: direction/forward
top level: Si-tag1
start: 1
end: 99
orientation: inline

Si-tag1
type: SequenceAnnotation
persistent identity: annotation2444372
display id: annotation2444372
version: 1
top level: Si-tag1
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1592007/annotation2444372/range2444372/1

https://synbiohub.org/public/igem/BBa_K1592007/annotation2444372/range2444372/1
type: Range
persistent identity: range2444372
display id: range2444372
version: 1
direction: direction/forward
top level: Si-tag1
start: 124
end: 303
orientation: inline

GS linker
type: SequenceAnnotation
persistent identity: annotation2444387
display id: annotation2444387
version: 1
top level: Si-tag1
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1592007/annotation2444387/range2444387/1

https://synbiohub.org/public/igem/BBa_K1592007/annotation2444387/range2444387/1
type: Range
persistent identity: range2444387
display id: range2444387
version: 1
direction: direction/forward
top level: Si-tag1
start: 310
end: 336
orientation: inline

NdeI
type: SequenceAnnotation
persistent identity: annotation2444388
display id: annotation2444388
version: 1
top level: Si-tag1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1592007/annotation2444388/range2444388/1

https://synbiohub.org/public/igem/BBa_K1592007/annotation2444388/range2444388/1
type: Range
persistent identity: range2444388
display id: range2444388
version: 1
direction: direction/forward
top level: Si-tag1
start: 337
end: 342
orientation: inline

BamHI
type: SequenceAnnotation
persistent identity: annotation2444371
display id: annotation2444371
version: 1
top level: Si-tag1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1592007/annotation2444371/range2444371/1

https://synbiohub.org/public/igem/BBa_K1592007/annotation2444371/range2444371/1
type: Range
persistent identity: range2444371
display id: range2444371
version: 1
direction: direction/forward
top level: Si-tag1
start: 100
end: 105
orientation: inline

BBa_K1689005
type: ComponentDefinition
persistent identity: BBa K1689005
display id: BBa_K1689005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Coding sequence of FKBP-Cluc398
creator: Xiang Li
created: 2015-09-15T11:00:00Z
modified: 2015-09-18T04:19:09Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2107_
m user id: 26445
owner id: 26464
owning group id: 9
status: status/Planning
bookmark: false
mutable description: including firefly luciferase C terminal 398 amino acid domain, 2??linker, and FKBP.
mutable notes: FKBP and C terminal 398 amino acid domain are linked by 2??linker
mutable provenance: E.coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: start codon
sequence annotation: FKBP
sequence annotation: B0034
sequence annotation: J23110
sequence annotation: stop codon
sequence annotation: C398 terminal of firefly luciferase
sequence annotation: GGGSGGGGSG linker
sequence: https://synbiohub.org/public/igem/BBa_K1689005_sequence/1

start codon
type: SequenceAnnotation
persistent identity: annotation2470122
display id: annotation2470122
version: 1
top level: BBa_K1689005
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1689005/annotation2470122/range2470122/1

https://synbiohub.org/public/igem/BBa_K1689005/annotation2470122/range2470122/1
type: Range
persistent identity: range2470122
display id: range2470122
version: 1
direction: direction/forward
top level: BBa_K1689005
start: 48
end: 50
orientation: inline

FKBP
type: SequenceAnnotation
persistent identity: annotation2470134
display id: annotation2470134
version: 1
top level: BBa_K1689005
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1689005/annotation2470134/range2470134/1

https://synbiohub.org/public/igem/BBa_K1689005/annotation2470134/range2470134/1
type: Range
persistent identity: range2470134
display id: range2470134
version: 1
direction: direction/forward
top level: BBa_K1689005
start: 51
end: 371
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2470121
display id: annotation2470121
version: 1
top level: BBa_K1689005
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1689005/annotation2470121/range2470121/1

https://synbiohub.org/public/igem/BBa_K1689005/annotation2470121/range2470121/1
type: Range
persistent identity: range2470121
display id: range2470121
version: 1
direction: direction/forward
top level: BBa_K1689005
start: 36
end: 47
orientation: inline

J23110
type: SequenceAnnotation
persistent identity: annotation2470118
display id: annotation2470118
version: 1
top level: BBa_K1689005
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1689005/annotation2470118/range2470118/1

https://synbiohub.org/public/igem/BBa_K1689005/annotation2470118/range2470118/1
type: Range
persistent identity: range2470118
display id: range2470118
version: 1
direction: direction/forward
top level: BBa_K1689005
start: 1
end: 35
orientation: inline

stop codon
type: SequenceAnnotation
persistent identity: annotation2470138
display id: annotation2470138
version: 1
top level: BBa_K1689005
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1689005/annotation2470138/range2470138/1

https://synbiohub.org/public/igem/BBa_K1689005/annotation2470138/range2470138/1
type: Range
persistent identity: range2470138
display id: range2470138
version: 1
direction: direction/forward
top level: BBa_K1689005
start: 861
end: 863
orientation: inline

C398 terminal of firefly luciferase
type: SequenceAnnotation
persistent identity: annotation2470137
display id: annotation2470137
version: 1
top level: BBa_K1689005
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1689005/annotation2470137/range2470137/1

https://synbiohub.org/public/igem/BBa_K1689005/annotation2470137/range2470137/1
type: Range
persistent identity: range2470137
display id: range2470137
version: 1
direction: direction/forward
top level: BBa_K1689005
start: 402
end: 860
orientation: inline

GGGSGGGGSG linker
type: SequenceAnnotation
persistent identity: annotation2470136
display id: annotation2470136
version: 1
top level: BBa_K1689005
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1689005/annotation2470136/range2470136/1

https://synbiohub.org/public/igem/BBa_K1689005/annotation2470136/range2470136/1
type: Range
persistent identity: range2470136
display id: range2470136
version: 1
direction: direction/forward
top level: BBa_K1689005
start: 372
end: 401
orientation: inline

BBa_K1758342
type: ComponentDefinition
persistent identity: BBa K1758342
display id: BBa_K1758342
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Mercury responsive promoter with UTR-sfGFP
creator: Team Bielefeld-CeBiTec 2015
created: 2015-08-28T11:00:00Z
modified: 2016-01-06T03:41:22Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2180_
m user id: 4206
owner id: 26630
owning group id: 9
status: status/Planning
bookmark: false
mutable description: mercury responsive promoter with 5??untranslated region and sfGFP for detection
mutable notes: ...
mutable provenance: modified after K346002
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: T7-term
sequence annotation: 5??UTR
sequence annotation: merT
sequence annotation: sfGFP
sequence annotation: T1
sequence: https://synbiohub.org/public/igem/BBa_K1758342_sequence/1

T7-term
type: SequenceAnnotation
persistent identity: annotation2439970
display id: annotation2439970
version: 1
top level: BBa_K1758342
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1758342/annotation2439970/range2439970/1

https://synbiohub.org/public/igem/BBa_K1758342/annotation2439970/range2439970/1
type: Range
persistent identity: range2439970
display id: range2439970
version: 1
direction: direction/forward
top level: BBa_K1758342
start: 926
end: 966
orientation: inline

5??UTR
type: SequenceAnnotation
persistent identity: annotation2439967
display id: annotation2439967
version: 1
top level: BBa_K1758342
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1758342/annotation2439967/range2439967/1

https://synbiohub.org/public/igem/BBa_K1758342/annotation2439967/range2439967/1
type: Range
persistent identity: range2439967
display id: range2439967
version: 1
direction: direction/forward
top level: BBa_K1758342
start: 64
end: 109
orientation: inline

merT
type: SequenceAnnotation
persistent identity: annotation2439966
display id: annotation2439966
version: 1
top level: BBa_K1758342
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1758342/annotation2439966/range2439966/1

https://synbiohub.org/public/igem/BBa_K1758342/annotation2439966/range2439966/1
type: Range
persistent identity: range2439966
display id: range2439966
version: 1
direction: direction/forward
top level: BBa_K1758342
start: 1
end: 57
orientation: inline

sfGFP
type: SequenceAnnotation
persistent identity: annotation2439968
display id: annotation2439968
version: 1
top level: BBa_K1758342
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1758342/annotation2439968/range2439968/1

https://synbiohub.org/public/igem/BBa_K1758342/annotation2439968/range2439968/1
type: Range
persistent identity: range2439968
display id: range2439968
version: 1
direction: direction/forward
top level: BBa_K1758342
start: 110
end: 829
orientation: inline

T1
type: SequenceAnnotation
persistent identity: annotation2439969
display id: annotation2439969
version: 1
top level: BBa_K1758342
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1758342/annotation2439969/range2439969/1

https://synbiohub.org/public/igem/BBa_K1758342/annotation2439969/range2439969/1
type: Range
persistent identity: range2439969
display id: range2439969
version: 1
direction: direction/forward
top level: BBa_K1758342
start: 838
end: 917
orientation: inline

BBa_K1632011
type: ComponentDefinition
persistent identity: BBa K1632011
display id: BBa_K1632011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: fimE (wild-type)
creator: Riku Shinohara
created: 2015-08-17T11:00:00Z
modified: 2015-09-15T01:08:25Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2049_
m user id: 24760
owner id: 22687
owning group id: 9
status: status/Planning
bookmark: false
mutable description: a
mutable notes: a
mutable provenance: a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1632011_sequence/1

Php4d
type: ComponentDefinition
persistent identity: BBa K1592004
display id: BBa_K1592004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: promoter hp4d
creator: Shuyan Tang
created: 2015-08-31T11:00:00Z
modified: 2015-09-18T09:25:27Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2009_
m user id: 20267
owner id: 20267
owning group id: 9
status: status/Planning
bookmark: false
mutable description: The recombinant promotor hp4d, nearly unaffected by pH, carbon source, nitrogen source and some conditions, can strongly promote gene expression in any culture medium(Madzak et al., 2000). Promoter hp4d is composed of four tandem repeated UAS1 sequences in upstream and the small LEU2 promoter, which is the sequence between TATA box and start condon ATG of LEU2 promoter in downstream. UAS1 is the upsteam activating sequence of the pXPR2, which is less affected by other conditions. Though any inducers are non-essential for activiation of php4d during cultivating, hp4d, activated by unknown element, isn't constitutive promoter. The gene promoted by promoter hp4d usually expresses at the early stage of stabilization, called growth phase dependent (Nicaud et al., 2002). This feature, separating the strain growth and producing foreign protein, is useful for production of foreign protein, which avoids the harm by expressing foreign protein.
mutable notes: No.
mutable provenance: From our lab.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: UAS1-3
sequence annotation: LEU
sequence annotation: UAS1-1
sequence annotation: UAS1-2
sequence annotation: UAS1-4
sequence: https://synbiohub.org/public/igem/BBa_K1592004_sequence/1

UAS1-3
type: SequenceAnnotation
persistent identity: annotation2441398
display id: annotation2441398
version: 1
top level: Php4d
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1592004/annotation2441398/range2441398/1

https://synbiohub.org/public/igem/BBa_K1592004/annotation2441398/range2441398/1
type: Range
persistent identity: range2441398
display id: range2441398
version: 1
direction: direction/forward
top level: Php4d
start: 242
end: 350
orientation: inline

LEU
type: SequenceAnnotation
persistent identity: annotation2441400
display id: annotation2441400
version: 1
top level: Php4d
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1592004/annotation2441400/range2441400/1

https://synbiohub.org/public/igem/BBa_K1592004/annotation2441400/range2441400/1
type: Range
persistent identity: range2441400
display id: range2441400
version: 1
direction: direction/forward
top level: Php4d
start: 477
end: 550
orientation: inline

UAS1-1
type: SequenceAnnotation
persistent identity: annotation2441397
display id: annotation2441397
version: 1
top level: Php4d
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1592004/annotation2441397/range2441397/1

https://synbiohub.org/public/igem/BBa_K1592004/annotation2441397/range2441397/1
type: Range
persistent identity: range2441397
display id: range2441397
version: 1
direction: direction/forward
top level: Php4d
start: 24
end: 132
orientation: inline

UAS1-2
type: SequenceAnnotation
persistent identity: annotation2441396
display id: annotation2441396
version: 1
top level: Php4d
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1592004/annotation2441396/range2441396/1

https://synbiohub.org/public/igem/BBa_K1592004/annotation2441396/range2441396/1
type: Range
persistent identity: range2441396
display id: range2441396
version: 1
direction: direction/forward
top level: Php4d
start: 133
end: 241
orientation: inline

UAS1-4
type: SequenceAnnotation
persistent identity: annotation2441399
display id: annotation2441399
version: 1
top level: Php4d
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1592004/annotation2441399/range2441399/1

https://synbiohub.org/public/igem/BBa_K1592004/annotation2441399/range2441399/1
type: Range
persistent identity: range2441399
display id: range2441399
version: 1
direction: direction/forward
top level: Php4d
start: 351
end: 453
orientation: inline

BBa_K1699005
type: ComponentDefinition
persistent identity: BBa K1699005
display id: BBa_K1699005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: gRNA for dCas9-VP64 targeting synthetic activation promoter pMLPm under U6 promoter
creator: Emil Ruvinov
created: 2015-08-08T11:00:00Z
modified: 2015-08-09T10:07:27Z
discontinued: false
dominant: false
group u list: _2117_
m user id: 24826
owner id: 24826
owning group id: 9
status: status/Planning
bookmark: false
mutable description: gRNA for dCas9-VP64 targeting synthetic activation promoter pMLPm under U6 promoter
mutable notes: Design
mutable provenance: Source
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: gRNA for pMLPm
sequence annotation: Human U6 promoter
sequence annotation: gRNA scaffold for SpCas9
sequence: https://synbiohub.org/public/igem/BBa_K1699005_sequence/1

gRNA for pMLPm
type: SequenceAnnotation
persistent identity: annotation2434622
display id: annotation2434622
version: 1
top level: BBa_K1699005
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1699005/annotation2434622/range2434622/1

https://synbiohub.org/public/igem/BBa_K1699005/annotation2434622/range2434622/1
type: Range
persistent identity: range2434622
display id: range2434622
version: 1
direction: direction/forward
top level: BBa_K1699005
start: 256
end: 275
orientation: inline

Human U6 promoter
type: SequenceAnnotation
persistent identity: annotation2434621
display id: annotation2434621
version: 1
top level: BBa_K1699005
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1699005/annotation2434621/range2434621/1

https://synbiohub.org/public/igem/BBa_K1699005/annotation2434621/range2434621/1
type: Range
persistent identity: range2434621
display id: range2434621
version: 1
direction: direction/forward
top level: BBa_K1699005
start: 1
end: 249
orientation: inline

gRNA scaffold for SpCas9
type: SequenceAnnotation
persistent identity: annotation2434623
display id: annotation2434623
version: 1
top level: BBa_K1699005
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1699005/annotation2434623/range2434623/1

https://synbiohub.org/public/igem/BBa_K1699005/annotation2434623/range2434623/1
type: Range
persistent identity: range2434623
display id: range2434623
version: 1
direction: direction/forward
top level: BBa_K1699005
start: 276
end: 359
orientation: inline

BBa_K1758300
type: ComponentDefinition
persistent identity: BBa K1758300
display id: BBa_K1758300
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: sfGFP controlled by T7 promoter and arsenic operator
creator: Team Bielefeld-CeBiTec 2015
created: 2015-08-21T11:00:00Z
modified: 2015-09-18T04:46:17Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2180_
m user id: 27935
owner id: 26587
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This part consists of sfGFP with a translation enhancing 5'-UTR under the control of the T7 promoter and arsenic operator. In combination with the E. coli arsenic repressor arsR, this part can be used as an arsenic biosensor.
mutable notes: This part was created by inserting the T7 promoter BBa_I719005 upstream of BBa_J33201 via 3A assembly. Subsequently, Gibson assembly was performed to replace arsR by sfGFP and introduce the UTR. The followoing primers and templates were used: Fragment 1: UTR_arsO_rev: ccttcttttttttttttaaagttaaacaaaattatTAAGTCAAAAACATATATGACTTAACG Cm_fwd: CGGCATCAGCACCTTGTC Template: pSB1C3-pT7-arsR Fragment 2: UTR_fwd: GTTTAACTTTAAAAAAAAAAAAGAAGGAGATATACATATGCGTAAAGGCGAAGAGCTGTTCAC Cm_rev: TATACGCAAGGCGACAAG Template: BBa_I746909
mutable provenance: Based on three parts from the distribution.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: ArsR binding site
sequence annotation: sfGFP
sequence annotation: BBa_B0034
sequence annotation: 5'-UTR
sequence annotation: BBa_I719005
sequence annotation: T7 promoter
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_K1758300_sequence/1

ArsR binding site
type: SequenceAnnotation
persistent identity: annotation2436871
display id: annotation2436871
version: 1
top level: BBa_K1758300
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K1758300/annotation2436871/range2436871/1

https://synbiohub.org/public/igem/BBa_K1758300/annotation2436871/range2436871/1
type: Range
persistent identity: range2436871
display id: range2436871
version: 1
direction: direction/forward
top level: BBa_K1758300
start: 67
end: 85
orientation: inline

sfGFP
type: SequenceAnnotation
persistent identity: annotation2436867
display id: annotation2436867
version: 1
top level: BBa_K1758300
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1758300/annotation2436867/range2436867/1

https://synbiohub.org/public/igem/BBa_K1758300/annotation2436867/range2436867/1
type: Range
persistent identity: range2436867
display id: range2436867
version: 1
direction: direction/forward
top level: BBa_K1758300
start: 150
end: 866
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2436868
display id: annotation2436868
version: 1
top level: BBa_K1758300
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1758300/annotation2436868/range2436868/1

https://synbiohub.org/public/igem/BBa_K1758300/annotation2436868/range2436868/1
type: Range
persistent identity: range2436868
display id: range2436868
version: 1
direction: direction/forward
top level: BBa_K1758300
start: 137
end: 144
orientation: inline

5'-UTR
type: SequenceAnnotation
persistent identity: annotation2436872
display id: annotation2436872
version: 1
top level: BBa_K1758300
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1758300/annotation2436872/range2436872/1

https://synbiohub.org/public/igem/BBa_K1758300/annotation2436872/range2436872/1
type: Range
persistent identity: range2436872
display id: range2436872
version: 1
direction: direction/forward
top level: BBa_K1758300
start: 104
end: 149
orientation: inline

BBa_I719005
type: SequenceAnnotation
persistent identity: annotation2436866
display id: annotation2436866
version: 1
top level: BBa_K1758300
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1758300/annotation2436866/range2436866/1

https://synbiohub.org/public/igem/BBa_K1758300/annotation2436866/range2436866/1
type: Range
persistent identity: range2436866
display id: range2436866
version: 1
direction: direction/forward
top level: BBa_K1758300
start: 1
end: 23
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2436865
display id: annotation2436865
version: 1
top level: BBa_K1758300
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1758300/annotation2436865/range2436865/1

https://synbiohub.org/public/igem/BBa_K1758300/annotation2436865/range2436865/1
type: Range
persistent identity: range2436865
display id: range2436865
version: 1
direction: direction/forward
top level: BBa_K1758300
start: 1
end: 23
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2436870
display id: annotation2436870
version: 1
top level: BBa_K1758300
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1758300/annotation2436870/range2436870/1

https://synbiohub.org/public/igem/BBa_K1758300/annotation2436870/range2436870/1
type: Range
persistent identity: range2436870
display id: range2436870
version: 1
direction: direction/forward
top level: BBa_K1758300
start: 966
end: 1006
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2436869
display id: annotation2436869
version: 1
top level: BBa_K1758300
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1758300/annotation2436869/range2436869/1

https://synbiohub.org/public/igem/BBa_K1758300/annotation2436869/range2436869/1
type: Range
persistent identity: range2436869
display id: range2436869
version: 1
direction: direction/forward
top level: BBa_K1758300
start: 878
end: 957
orientation: inline

pFig2c
type: ComponentDefinition
persistent identity: BBa K1829002
display id: BBa_K1829002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pFig2c: Mating factor alpha responsive promoter
creator: Nicholas Suen
created: 2015-09-08T11:00:00Z
modified: 2015-09-14T11:40:07Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2255_
m user id: 18557
owner id: 27842
owning group id: 9
status: status/Planning
bookmark: false
mutable description: pFig2c is a mating factor alpha responsive promoter, meaning that it is activated by the presence of mating factor alpha. It is endogenous in S. Cerevisiae cells and is followed by the Fig2 gene. The Fig2 gene is a part of the mating pathway in S. Cerevisiae, it contributes to maintaining cell wall integrity when the cells project ("shmoo") towards each other.
mutable notes: We changed nucleeotide 22 from adenine to thymine in order to eliminate the illegal Pst1 cut site.
mutable provenance: pFig2c is found in the genomic sequence of MATa S. cerevisiae.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K1829002_sequence/1

BBa_K1639001
type: ComponentDefinition
persistent identity: BBa K1639001
display id: BBa_K1639001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Trigger RNA
creator: Mustafa Yılmaz
created: 2015-09-11T11:00:00Z
modified: 2015-09-18T06:50:31Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2056_
m user id: 20835
owner id: 20835
owning group id: 9
status: status/Planning
bookmark: true
mutable description: This part required for producing reporter flourescence protein GFP from Toehold-GFP(BBa_K1639000) composite part.
mutable notes: This part synthetase with strong T7 Promoter downstream of Trigger RNA. There is also a T7 Terminator after Trigger RNA.
mutable provenance: Wyss Institute for Biologically Inspired Engineering. Sequence is taken from supplementary data of article: "Toehold Switches: De-Novo-Designed Regulators of Gene Expression". (PMID: 25417166)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence annotation: T7 Terminator
sequence annotation: T7 Promoter
sequence annotation: Trigger RNA
sequence: https://synbiohub.org/public/igem/BBa_K1639001_sequence/1

T7 Terminator
type: SequenceAnnotation
persistent identity: annotation2452876
display id: annotation2452876
version: 1
top level: BBa_K1639001
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1639001/annotation2452876/range2452876/1

https://synbiohub.org/public/igem/BBa_K1639001/annotation2452876/range2452876/1
type: Range
persistent identity: range2452876
display id: range2452876
version: 1
direction: direction/forward
top level: BBa_K1639001
start: 84
end: 130
orientation: inline

T7 Promoter
type: SequenceAnnotation
persistent identity: annotation2452874
display id: annotation2452874
version: 1
top level: BBa_K1639001
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1639001/annotation2452874/range2452874/1

https://synbiohub.org/public/igem/BBa_K1639001/annotation2452874/range2452874/1
type: Range
persistent identity: range2452874
display id: range2452874
version: 1
direction: direction/forward
top level: BBa_K1639001
start: 1
end: 19
orientation: inline

Trigger RNA
type: SequenceAnnotation
persistent identity: annotation2452875
display id: annotation2452875
version: 1
top level: BBa_K1639001
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1639001/annotation2452875/range2452875/1

https://synbiohub.org/public/igem/BBa_K1639001/annotation2452875/range2452875/1
type: Range
persistent identity: range2452875
display id: range2452875
version: 1
direction: direction/forward
top level: BBa_K1639001
start: 22
end: 83
orientation: inline

BBa_K1806005
type: ComponentDefinition
persistent identity: BBa K1806005
display id: BBa_K1806005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7+ ibPB RNA Thermometer + RFP
creator: İBRAHİM YASİR ORHAN
created: 2015-09-16T11:00:00Z
modified: 2015-09-21T11:27:36Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _973_2231_
m user id: 11156
owner id: 11153
owning group id: 9
status: status/Planning
bookmark: true
mutable description: The part is designed to produce RFP in higher temperatures. The RNA Thermometer acts as a heat-shock RBS.
mutable notes: none
mutable provenance: The part is composed of distinct parts from various origins.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence: https://synbiohub.org/public/igem/BBa_K1806005_sequence/1

BBa_K1758325
type: ComponentDefinition
persistent identity: BBa K1758325
display id: BBa_K1758325
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Copper responsive promoter with T7-promoter and UTR-sfGFP
creator: Team Bielefeld-CeBiTec 2015
created: 2015-09-07T11:00:00Z
modified: 2016-01-06T03:42:09Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2180_
m user id: 4206
owner id: 26630
owning group id: 9
status: status/Planning
bookmark: false
mutable description: ...
mutable notes: ...
mutable provenance: ...
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_I719005
component: BBa_K1758323
sequence annotation: BBa_K1758323
sequence annotation: BBa_I719005
sequence: https://synbiohub.org/public/igem/BBa_K1758325_sequence/1

BBa_I719005
type: Component
persistent identity: component2447482
display id: component2447482
version: 1
top level: BBa_K1758325
access: public
definition: https://synbiohub.org/public/igem/BBa_I719005/1

BBa_K1758323
type: Component
persistent identity: component2447488
display id: component2447488
version: 1
top level: BBa_K1758325
access: public
definition: BBa_K1758323

BBa_K1758323
type: SequenceAnnotation
persistent identity: annotation2447488
display id: annotation2447488
version: 1
top level: BBa_K1758325
location: https://synbiohub.org/public/igem/BBa_K1758325/annotation2447488/range2447488/1
component: BBa_K1758323

https://synbiohub.org/public/igem/BBa_K1758325/annotation2447488/range2447488/1
type: Range
persistent identity: range2447488
display id: range2447488
version: 1
direction: direction/reverse
top level: BBa_K1758325
start: 32
end: 1023
orientation: inline

BBa_I719005
type: SequenceAnnotation
persistent identity: annotation2447482
display id: annotation2447482
version: 1
top level: BBa_K1758325
location: https://synbiohub.org/public/igem/BBa_K1758325/annotation2447482/range2447482/1
component: BBa_I719005

https://synbiohub.org/public/igem/BBa_K1758325/annotation2447482/range2447482/1
type: Range
persistent identity: range2447482
display id: range2447482
version: 1
direction: direction/reverse
top level: BBa_K1758325
start: 1
end: 23
orientation: inline

BBa_K1758330
type: ComponentDefinition
persistent identity: BBa K1758330
display id: BBa_K1758330
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lead repressor under control of constitutive promoter and strong RBS
creator: Team Bielefeld-CeBiTec 2015
created: 2015-08-29T11:00:00Z
modified: 2016-01-25T11:24:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2180_
m user id: 4206
owner id: 26630
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Lead promoter repressor under the control of konstitutive promoter (K608002)
mutable notes: ...
mutable provenance: ...
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: K608002
sequence annotation: PbrR
sequence: https://synbiohub.org/public/igem/BBa_K1758330_sequence/1

K608002
type: SequenceAnnotation
persistent identity: annotation2440078
display id: annotation2440078
version: 1
top level: BBa_K1758330
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1758330/annotation2440078/range2440078/1

https://synbiohub.org/public/igem/BBa_K1758330/annotation2440078/range2440078/1
type: Range
persistent identity: range2440078
display id: range2440078
version: 1
direction: direction/reverse
top level: BBa_K1758330
start: 444
end: 499
orientation: inline

PbrR
type: SequenceAnnotation
persistent identity: annotation2440079
display id: annotation2440079
version: 1
top level: BBa_K1758330
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1758330/annotation2440079/range2440079/1

https://synbiohub.org/public/igem/BBa_K1758330/annotation2440079/range2440079/1
type: Range
persistent identity: range2440079
display id: range2440079
version: 1
direction: direction/reverse
top level: BBa_K1758330
start: 1
end: 438
orientation: inline

BBa_K1758376
type: ComponentDefinition
persistent identity: BBa K1758376
display id: BBa_K1758376
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GHB / GBL biosensor - reporter part
creator: Team Bielefeld-CeBiTec 2015
created: 2015-08-31T11:00:00Z
modified: 2015-09-19T01:22:36Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2180_
m user id: 26563
owner id: 26563
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This part contains all features from [http://parts.igem.org/Part:BBa_K1758102 BBa_K1758102] plus the blc-operator site described in [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4083662/ Pan et al. 2013]. BlcR (see http://parts.igem.org/Part:BBa_K1758370 K1758370]) can bind to the operator site.
mutable notes: ---
mutable provenance: This part was assembled via PCR with Gibson primers on [http://parts.igem.org/Part:BBa_K1758102 BBa_K1758102]
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: B0010
sequence annotation: silent mutation
sequence annotation: Blc operator
sequence annotation: sfGFP
sequence annotation: 5' -UTR
sequence annotation: B0012
sequence annotation: K1758100
sequence annotation: K1758101
sequence annotation: T7 promoter
sequence: https://synbiohub.org/public/igem/BBa_K1758376_sequence/1

B0010
type: SequenceAnnotation
persistent identity: annotation2441683
display id: annotation2441683
version: 1
top level: BBa_K1758376
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1758376/annotation2441683/range2441683/1

https://synbiohub.org/public/igem/BBa_K1758376/annotation2441683/range2441683/1
type: Range
persistent identity: range2441683
display id: range2441683
version: 1
direction: direction/forward
top level: BBa_K1758376
start: 838
end: 917
orientation: inline

silent mutation
type: SequenceAnnotation
persistent identity: annotation2441694
display id: annotation2441694
version: 1
top level: BBa_K1758376
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K1758376/annotation2441694/range2441694/1

https://synbiohub.org/public/igem/BBa_K1758376/annotation2441694/range2441694/1
type: Range
persistent identity: range2441694
display id: range2441694
version: 1
direction: direction/forward
top level: BBa_K1758376
start: 691
end: 691
orientation: inline

Blc operator
type: SequenceAnnotation
persistent identity: annotation2441678
display id: annotation2441678
version: 1
top level: BBa_K1758376
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K1758376/annotation2441678/range2441678/1

https://synbiohub.org/public/igem/BBa_K1758376/annotation2441678/range2441678/1
type: Range
persistent identity: range2441678
display id: range2441678
version: 1
direction: direction/forward
top level: BBa_K1758376
start: 23
end: 63
orientation: inline

sfGFP
type: SequenceAnnotation
persistent identity: annotation2441682
display id: annotation2441682
version: 1
top level: BBa_K1758376
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1758376/annotation2441682/range2441682/1

https://synbiohub.org/public/igem/BBa_K1758376/annotation2441682/range2441682/1
type: Range
persistent identity: range2441682
display id: range2441682
version: 1
direction: direction/forward
top level: BBa_K1758376
start: 110
end: 829
orientation: inline

5' -UTR
type: SequenceAnnotation
persistent identity: annotation2441679
display id: annotation2441679
version: 1
top level: BBa_K1758376
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1758376/annotation2441679/range2441679/1

https://synbiohub.org/public/igem/BBa_K1758376/annotation2441679/range2441679/1
type: Range
persistent identity: range2441679
display id: range2441679
version: 1
direction: direction/forward
top level: BBa_K1758376
start: 64
end: 109
orientation: inline

B0012
type: SequenceAnnotation
persistent identity: annotation2441684
display id: annotation2441684
version: 1
top level: BBa_K1758376
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1758376/annotation2441684/range2441684/1

https://synbiohub.org/public/igem/BBa_K1758376/annotation2441684/range2441684/1
type: Range
persistent identity: range2441684
display id: range2441684
version: 1
direction: direction/forward
top level: BBa_K1758376
start: 926
end: 966
orientation: inline

K1758100
type: SequenceAnnotation
persistent identity: annotation2441680
display id: annotation2441680
version: 1
top level: BBa_K1758376
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1758376/annotation2441680/range2441680/1

https://synbiohub.org/public/igem/BBa_K1758376/annotation2441680/range2441680/1
type: Range
persistent identity: range2441680
display id: range2441680
version: 1
direction: direction/forward
top level: BBa_K1758376
start: 64
end: 109
orientation: inline

K1758101
type: SequenceAnnotation
persistent identity: annotation2441681
display id: annotation2441681
version: 1
top level: BBa_K1758376
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1758376/annotation2441681/range2441681/1

https://synbiohub.org/public/igem/BBa_K1758376/annotation2441681/range2441681/1
type: Range
persistent identity: range2441681
display id: range2441681
version: 1
direction: direction/forward
top level: BBa_K1758376
start: 64
end: 966
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2441677
display id: annotation2441677
version: 1
top level: BBa_K1758376
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1758376/annotation2441677/range2441677/1

https://synbiohub.org/public/igem/BBa_K1758376/annotation2441677/range2441677/1
type: Range
persistent identity: range2441677
display id: range2441677
version: 1
direction: direction/forward
top level: BBa_K1758376
start: 1
end: 22
orientation: inline

BBa_K1799000
type: ComponentDefinition
persistent identity: BBa K1799000
display id: BBa_K1799000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pLsrA-2
creator: Miranda Halle
created: 2015-08-05T11:00:00Z
modified: 2015-09-18T01:13:21Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2224_
m user id: 9151
owner id: 19613
owning group id: 9
status: status/Planning
bookmark: true
mutable description: Promoter for the E. coli LsrACDB operon. This promoter is repressed by LsrR, and derepressed by autoinducer 2 (AI-2) in the presence of LsrK.
mutable notes: Promoter begins with the start of its cAMP-CRP binding site and ends after the second key LsrR binding sequence.
mutable provenance: This part was synthesized from E. coli genomic data retrieved via EcoGene. It is based on, but not identical to, a part created by the 2011 Tokyo Tech iGEM team: http://parts.igem.org/Part:BBa_K649100
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: LsrR binding site
sequence annotation: cAMP-CRP binding site
sequence: https://synbiohub.org/public/igem/BBa_K1799000_sequence/1

LsrR binding site
type: SequenceAnnotation
persistent identity: annotation2434487
display id: annotation2434487
version: 1
top level: BBa_K1799000
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K1799000/annotation2434487/range2434487/1

https://synbiohub.org/public/igem/BBa_K1799000/annotation2434487/range2434487/1
type: Range
persistent identity: range2434487
display id: range2434487
version: 1
direction: direction/forward
top level: BBa_K1799000
start: 94
end: 102
orientation: inline

cAMP-CRP binding site
type: SequenceAnnotation
persistent identity: annotation2434485
display id: annotation2434485
version: 1
top level: BBa_K1799000
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K1799000/annotation2434485/range2434485/1

https://synbiohub.org/public/igem/BBa_K1799000/annotation2434485/range2434485/1
type: Range
persistent identity: range2434485
display id: range2434485
version: 1
direction: direction/forward
top level: BBa_K1799000
start: 1
end: 21
orientation: inline

LsrR binding site
type: SequenceAnnotation
persistent identity: annotation2434486
display id: annotation2434486
version: 1
top level: BBa_K1799000
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K1799000/annotation2434486/range2434486/1

https://synbiohub.org/public/igem/BBa_K1799000/annotation2434486/range2434486/1
type: Range
persistent identity: range2434486
display id: range2434486
version: 1
direction: direction/forward
top level: BBa_K1799000
start: 81
end: 86
orientation: inline

BBa_K1799016
type: ComponentDefinition
persistent identity: BBa K1799016
display id: BBa_K1799016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NsrR open reading frame
creator: Will Shindel
created: 2015-08-26T11:00:00Z
modified: 2015-09-17T02:18:38Z
discontinued: false
dominant: false
group u list: _2224_
m user id: 26954
owner id: 26954
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Coding sequence for nitric oxide-sensitive repressor of pYeaR.
mutable notes: None
mutable provenance: This part was synthesized from E. coli genomic data retrieved via EcoGene.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1799016_sequence/1

RBS-xylB
type: ComponentDefinition
persistent identity: BBa K1602000
display id: BBa_K1602000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0034-xylB
creator: Jonas Sindlinger, Leon Kraus, Jonathan Pletzer-Zelgert, Elena Tonchevska, Sebastian Holzner, Mirjam Haun, Sebastian Barthel
created: 2015-08-27T11:00:00Z
modified: 2015-09-25T01:14:47Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2019_
m user id: 12512
owner id: 25989
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: BBa_IA Itaconic acid is an organic, dicarboxylic acid that is biotechnologically synthesized in Aspergillus terreus. It is derived from citric acid via 2 intermediates and a final decarboxylation. To enable this pathway in Escherichia coli it is necessary to introduce 3 genes. 2 of which are already established in the citric acid cycle and one that is from Apergillus terreus, a cis-aconitate decarboxylase (cadA). Figure 1 Reaction scheme of the itaconic acid producing operon. The substrates for the reaction are acetyl-coa and oxaloacetate. To simplify the process we are only looking at oxaloacetate right now. Oxaloacetate is metabolized to itaconic acid in 3 steps. ===Usage=== This part is a composite of three genes, each with a strong RBS (BBa_B0034) and all of them under control of one T7 Promoter (BBa_K1497017). citrate synthase - gltA (BBa_K1033001) aconitate hydratase 1 - acnA (BBa_K1033000) cis-aconitate decarboxylase - cadA (BBa_K1497100) iGEM TU Darmstadt 2014 :) Figure 2 Genetic map of the itaconic acid producing operon with T7 promoter. This brick directs the flux towards and finally enables E.Coli BL21 cells to synthesize itaconic acid in presence of the inductor IPTG.
mutable notes: BLANK
mutable provenance: GenBank
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_K1602009
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_K1602009
sequence: https://synbiohub.org/public/igem/BBa_K1602000_sequence/1

BBa_K1602009
type: Component
persistent identity: component2463050
display id: component2463050
version: 1
top level: RBS-xylB
access: public
definition: BBa_K1602009

BBa_B0034
type: Component
persistent identity: component2463049
display id: component2463049
version: 1
top level: RBS-xylB
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2463049
display id: annotation2463049
version: 1
top level: RBS-xylB
location: https://synbiohub.org/public/igem/BBa_K1602000/annotation2463049/range2463049/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1602000/annotation2463049/range2463049/1
type: Range
persistent identity: range2463049
display id: range2463049
version: 1
direction: direction/reverse
top level: RBS-xylB
start: 1
end: 12
orientation: inline

BBa_K1602009
type: SequenceAnnotation
persistent identity: annotation2463050
display id: annotation2463050
version: 1
top level: RBS-xylB
location: https://synbiohub.org/public/igem/BBa_K1602000/annotation2463050/range2463050/1
component: BBa_K1602009

https://synbiohub.org/public/igem/BBa_K1602000/annotation2463050/range2463050/1
type: Range
persistent identity: range2463050
display id: range2463050
version: 1
direction: direction/reverse
top level: RBS-xylB
start: 19
end: 765
orientation: inline

Si-tag1+li
type: ComponentDefinition
persistent identity: BBa K1592013
display id: BBa_K1592013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LIP prepro + E. coli ribosomal protein L2 (1-60,GS linker,202-273aa) + YLcwp3
creator: Shuyan Tang
created: 2015-09-07T11:00:00Z
modified: 2015-09-18T09:36:35Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2009_
m user id: 20267
owner id: 20267
owning group id: 9
status: status/Planning
bookmark: true
mutable description: __NOTOC__ BBa_K1592008 short This is the cell display system of Yarrowia lipolytica, composed of LIP prepro, interest protein, and YLcwp3.LIP prepro is signal peptide used to secrete the interest protein out of the cell, and the YLcwp3 is the anchor domain binding the interest protein to the cell wall of yeast.We use this system to display silica-tag and test its binding characteristics. Here we use this system to display Silica-tag on cell wall of Yarrowia lipolytica to binding the silica. E.coli ribosomal protein L2 was found to bind tightly to silicon particles, which have surfaces that are oxidized to silica. This L2 silica-binding tag, called the 'Si-tag', can be used for one-step targeting of functional proteins on silica surfaces. The silica-binding domains of E. coli L2 was mapped to amino acids 1???60, 61-202 and 203???273, called Si-tag1, Si-tag2 and Si-tag3. We respectively test the silica-binding characteristics of this three regions and their combinations.
mutable notes: In order to replace the domains more conveniently, we respectivey added BamHI, SalI, NdeI between LIP2 prepro, E. coli ribosomal protein L2, GS linker and YLcwp3
mutable provenance: E. coli ribosomal protein L2 was synthesized by IDT, LIP2 prepro and YLcwp3 were cloning from the plasmid JMP62, and we fused this three sequences.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: LIP2 prepro
sequence annotation: NdeI
sequence annotation: SalI
sequence annotation: GS Linker
sequence annotation: His6 tag
sequence annotation: BamHI
sequence annotation: Si-tag 1L3
sequence annotation: YLcwp3
sequence: https://synbiohub.org/public/igem/BBa_K1592013_sequence/1

LIP2 prepro
type: SequenceAnnotation
persistent identity: annotation2456166
display id: annotation2456166
version: 1
top level: Si-tag1+li
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1592013/annotation2456166/range2456166/1

https://synbiohub.org/public/igem/BBa_K1592013/annotation2456166/range2456166/1
type: Range
persistent identity: range2456166
display id: range2456166
version: 1
direction: direction/forward
top level: Si-tag1+li
start: 1
end: 99
orientation: inline

NdeI
type: SequenceAnnotation
persistent identity: annotation2456173
display id: annotation2456173
version: 1
top level: Si-tag1+li
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1592013/annotation2456173/range2456173/1

https://synbiohub.org/public/igem/BBa_K1592013/annotation2456173/range2456173/1
type: Range
persistent identity: range2456173
display id: range2456173
version: 1
direction: direction/forward
top level: Si-tag1+li
start: 586
end: 591
orientation: inline

SalI
type: SequenceAnnotation
persistent identity: annotation2456171
display id: annotation2456171
version: 1
top level: Si-tag1+li
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1592013/annotation2456171/range2456171/1

https://synbiohub.org/public/igem/BBa_K1592013/annotation2456171/range2456171/1
type: Range
persistent identity: range2456171
display id: range2456171
version: 1
direction: direction/forward
top level: Si-tag1+li
start: 535
end: 540
orientation: inline

GS Linker
type: SequenceAnnotation
persistent identity: annotation2463874
display id: annotation2463874
version: 1
top level: Si-tag1+li
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1592013/annotation2463874/range2463874/1

https://synbiohub.org/public/igem/BBa_K1592013/annotation2463874/range2463874/1
type: Range
persistent identity: range2463874
display id: range2463874
version: 1
direction: direction/forward
top level: Si-tag1+li
start: 304
end: 318
orientation: inline

GS Linker
type: SequenceAnnotation
persistent identity: annotation2456172
display id: annotation2456172
version: 1
top level: Si-tag1+li
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1592013/annotation2456172/range2456172/1

https://synbiohub.org/public/igem/BBa_K1592013/annotation2456172/range2456172/1
type: Range
persistent identity: range2456172
display id: range2456172
version: 1
direction: direction/forward
top level: Si-tag1+li
start: 541
end: 585
orientation: inline

His6 tag
type: SequenceAnnotation
persistent identity: annotation2456169
display id: annotation2456169
version: 1
top level: Si-tag1+li
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1592013/annotation2456169/range2456169/1

https://synbiohub.org/public/igem/BBa_K1592013/annotation2456169/range2456169/1
type: Range
persistent identity: range2456169
display id: range2456169
version: 1
direction: direction/forward
top level: Si-tag1+li
start: 106
end: 123
orientation: inline

BamHI
type: SequenceAnnotation
persistent identity: annotation2456168
display id: annotation2456168
version: 1
top level: Si-tag1+li
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1592013/annotation2456168/range2456168/1

https://synbiohub.org/public/igem/BBa_K1592013/annotation2456168/range2456168/1
type: Range
persistent identity: range2456168
display id: range2456168
version: 1
direction: direction/forward
top level: Si-tag1+li
start: 100
end: 105
orientation: inline

Si-tag 1L3
type: SequenceAnnotation
persistent identity: annotation2456170
display id: annotation2456170
version: 1
top level: Si-tag1+li
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1592013/annotation2456170/range2456170/1

https://synbiohub.org/public/igem/BBa_K1592013/annotation2456170/range2456170/1
type: Range
persistent identity: range2456170
display id: range2456170
version: 1
direction: direction/forward
top level: Si-tag1+li
start: 124
end: 534
orientation: inline

YLcwp3
type: SequenceAnnotation
persistent identity: annotation2456174
display id: annotation2456174
version: 1
top level: Si-tag1+li
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1592013/annotation2456174/range2456174/1

https://synbiohub.org/public/igem/BBa_K1592013/annotation2456174/range2456174/1
type: Range
persistent identity: range2456174
display id: range2456174
version: 1
direction: direction/forward
top level: Si-tag1+li
start: 592
end: 957
orientation: inline

BBa_K1650037
type: ComponentDefinition
persistent identity: BBa K1650037
display id: BBa_K1650037
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SpyCatcher
creator: Alexandra Richter
created: 2015-09-17T11:00:00Z
modified: 2015-09-18T11:03:58Z
discontinued: false
dominant: false
group u list: _2067_
m user id: 12520
owner id: 27246
owning group id: 9
status: status/Planning
bookmark: false
mutable description: part of the SpySystem
mutable notes: test
mutable provenance: test
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: BBa_K1650037
sequence: https://synbiohub.org/public/igem/BBa_K1650037_sequence/1

BBa_K1650037
type: SequenceAnnotation
persistent identity: annotation2474920
display id: annotation2474920
version: 1
top level: BBa_K1650037
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1650037/annotation2474920/range2474920/1

https://synbiohub.org/public/igem/BBa_K1650037/annotation2474920/range2474920/1
type: Range
persistent identity: range2474920
display id: range2474920
version: 1
direction: direction/forward
top level: BBa_K1650037
start: 1
end: 417
orientation: inline

BBa_K1666002
type: ComponentDefinition
persistent identity: BBa K1666002
display id: BBa_K1666002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LsrC of LuxS/AI-2 signaling pathway in Salmonalla
creator: Heming Wang
created: 2015-09-05T11:00:00Z
modified: 2015-09-13T04:06:04Z
discontinued: false
dominant: false
group u list: _2084_
m user id: 28478
owner id: 23948
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Quorum sensing is a process of bacterial cell-to-cell communication involving the production and detection of extracellular signaling molecules called autoinducers. And autoinducer-2 (AI-2) has been proposed to serve as a 'universal signal' for interspecies communication. In the LuxS/AI-2 signaling system of Salmonella Typhimurium, AI-2 response involves ATP binding cassette transporter encoded by genes named Lsr (LuxS regulated). And LsrC is thetransmembrane protein which constructed the ABC transport. In our project, we set this protein-coding part under a nisA promoter and try to integrate them in the genome of Lactobacillus or Lactococcus for the final purpose of constructing an integrated AI-2 response pathway of Salmonella in the engineered bacteria.
mutable notes: The lsrC gene was isolated from the genome of Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 using PCR. We added restriction enzyme sites when designing primers, and the assembly is done through the use of restriction sites (cutting and ligating).
mutable provenance: We extracted the genomic DNA of Salmonella enterica subsp. enterica serovar Typhimurium str. LT2, and used PCR to isolate the gene LsrA(Gene ID: 1255602).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: lsrC
sequence: https://synbiohub.org/public/igem/BBa_K1666002_sequence/1

lsrC
type: SequenceAnnotation
persistent identity: annotation2445156
display id: annotation2445156
version: 1
top level: BBa_K1666002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1666002/annotation2445156/range2445156/1

https://synbiohub.org/public/igem/BBa_K1666002/annotation2445156/range2445156/1
type: Range
persistent identity: range2445156
display id: range2445156
version: 1
direction: direction/forward
top level: BBa_K1666002
start: 1
end: 1089
orientation: inline

BBa_K1689016
type: ComponentDefinition
persistent identity: BBa K1689016
display id: BBa_K1689016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: dCas9-F[1,2]
creator: Wei weijia
created: 2015-09-15T11:00:00Z
modified: 2015-09-27T01:35:06Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2107_
m user id: 26531
owner id: 26531
owning group id: 9
status: status/Planning
bookmark: false
mutable description: pSB1C3-6His-dCas9-F[1,2], this part is a fusion protein of dCas9-F[1,2], the His tag is used for protein purification
mutable notes: dCas9 fusion protein coding sequence is too long, so you'd better amplify it separately.
mutable provenance: E.coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: GGGGS*2 Linker
sequence annotation: F[1,2]-dCas9
sequence annotation: F[1,2]
sequence annotation: stop
sequence annotation: dCas9
sequence annotation: His tag
sequence annotation: random spacer
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_K1689016_sequence/1

GGGGS*2 Linker
type: SequenceAnnotation
persistent identity: annotation2469960
display id: annotation2469960
version: 1
top level: BBa_K1689016
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1689016/annotation2469960/range2469960/1

https://synbiohub.org/public/igem/BBa_K1689016/annotation2469960/range2469960/1
type: Range
persistent identity: range2469960
display id: range2469960
version: 1
direction: direction/forward
top level: BBa_K1689016
start: 4156
end: 4185
orientation: inline

F[1,2]-dCas9
type: SequenceAnnotation
persistent identity: annotation2469957
display id: annotation2469957
version: 1
top level: BBa_K1689016
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1689016/annotation2469957/range2469957/1

https://synbiohub.org/public/igem/BBa_K1689016/annotation2469957/range2469957/1
type: Range
persistent identity: range2469957
display id: range2469957
version: 1
direction: direction/forward
top level: BBa_K1689016
start: 34
end: 4449
orientation: inline

F[1,2]
type: SequenceAnnotation
persistent identity: annotation2469962
display id: annotation2469962
version: 1
top level: BBa_K1689016
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1689016/annotation2469962/range2469962/1

https://synbiohub.org/public/igem/BBa_K1689016/annotation2469962/range2469962/1
type: Range
persistent identity: range2469962
display id: range2469962
version: 1
direction: direction/forward
top level: BBa_K1689016
start: 4186
end: 4446
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2477787
display id: annotation2477787
version: 1
top level: BBa_K1689016
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1689016/annotation2477787/range2477787/1

https://synbiohub.org/public/igem/BBa_K1689016/annotation2477787/range2477787/1
type: Range
persistent identity: range2477787
display id: range2477787
version: 1
direction: direction/forward
top level: BBa_K1689016
start: 4447
end: 4449
orientation: inline

dCas9
type: SequenceAnnotation
persistent identity: annotation2469959
display id: annotation2469959
version: 1
top level: BBa_K1689016
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1689016/annotation2469959/range2469959/1

https://synbiohub.org/public/igem/BBa_K1689016/annotation2469959/range2469959/1
type: Range
persistent identity: range2469959
display id: range2469959
version: 1
direction: direction/forward
top level: BBa_K1689016
start: 55
end: 4155
orientation: inline

His tag
type: SequenceAnnotation
persistent identity: annotation2469958
display id: annotation2469958
version: 1
top level: BBa_K1689016
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1689016/annotation2469958/range2469958/1

https://synbiohub.org/public/igem/BBa_K1689016/annotation2469958/range2469958/1
type: Range
persistent identity: range2469958
display id: range2469958
version: 1
direction: direction/forward
top level: BBa_K1689016
start: 37
end: 54
orientation: inline

random spacer
type: SequenceAnnotation
persistent identity: annotation2477788
display id: annotation2477788
version: 1
top level: BBa_K1689016
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1689016/annotation2477788/range2477788/1

https://synbiohub.org/public/igem/BBa_K1689016/annotation2477788/range2477788/1
type: Range
persistent identity: range2477788
display id: range2477788
version: 1
direction: direction/forward
top level: BBa_K1689016
start: 4450
end: 4520
orientation: inline

random spacer
type: SequenceAnnotation
persistent identity: annotation2477785
display id: annotation2477785
version: 1
top level: BBa_K1689016
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1689016/annotation2477785/range2477785/1

https://synbiohub.org/public/igem/BBa_K1689016/annotation2477785/range2477785/1
type: Range
persistent identity: range2477785
display id: range2477785
version: 1
direction: direction/forward
top level: BBa_K1689016
start: 1
end: 33
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2477786
display id: annotation2477786
version: 1
top level: BBa_K1689016
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1689016/annotation2477786/range2477786/1

https://synbiohub.org/public/igem/BBa_K1689016/annotation2477786/range2477786/1
type: Range
persistent identity: range2477786
display id: range2477786
version: 1
direction: direction/forward
top level: BBa_K1689016
start: 34
end: 36
orientation: inline

BBa_K1758107
type: ComponentDefinition
persistent identity: BBa K1758107
display id: BBa_K1758107
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SYFP2 under T7 promoter + RBS
creator: Team Bielefeld-CeBiTec 2015
created: 2015-08-28T11:00:00Z
modified: 2015-09-18T04:42:23Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2180_
m user id: 27935
owner id: 26563
owning group id: 9
status: status/Planning
bookmark: false
mutable description: SYFP2 is synthesized when T7-polymerase is induced.
mutable notes: ---
mutable provenance: 3A assembly of [http://parts.igem.org/Part:BBa_K525998 BBa_K525998] and [http://parts.igem.org/Part:BBa_K864100 BBa_K864100]
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K864100
component: BBa_K525998
sequence annotation: BBa_K864100
sequence annotation: BBa_K525998
sequence: https://synbiohub.org/public/igem/BBa_K1758107_sequence/1

BBa_K864100
type: Component
persistent identity: component2439949
display id: component2439949
version: 1
top level: BBa_K1758107
access: public
definition: https://synbiohub.org/public/igem/BBa_K864100/1

BBa_K525998
type: Component
persistent identity: component2439947
display id: component2439947
version: 1
top level: BBa_K1758107
access: public
definition: https://synbiohub.org/public/igem/BBa_K525998/1

BBa_K864100
type: SequenceAnnotation
persistent identity: annotation2439949
display id: annotation2439949
version: 1
top level: BBa_K1758107
location: https://synbiohub.org/public/igem/BBa_K1758107/annotation2439949/range2439949/1
component: BBa_K864100

https://synbiohub.org/public/igem/BBa_K1758107/annotation2439949/range2439949/1
type: Range
persistent identity: range2439949
display id: range2439949
version: 1
direction: direction/reverse
top level: BBa_K1758107
start: 39
end: 761
orientation: inline

BBa_K525998
type: SequenceAnnotation
persistent identity: annotation2439947
display id: annotation2439947
version: 1
top level: BBa_K1758107
location: https://synbiohub.org/public/igem/BBa_K1758107/annotation2439947/range2439947/1
component: BBa_K525998

https://synbiohub.org/public/igem/BBa_K1758107/annotation2439947/range2439947/1
type: Range
persistent identity: range2439947
display id: range2439947
version: 1
direction: direction/reverse
top level: BBa_K1758107
start: 1
end: 32
orientation: inline

BBa_K1758321
type: ComponentDefinition
persistent identity: BBa K1758321
display id: BBa_K1758321
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: mRFP under control of copper responsive promoter
creator: Team Bielefeld-CeBiTec 2015
created: 2015-08-28T11:00:00Z
modified: 2016-01-06T03:42:39Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2180_
m user id: 4206
owner id: 26630
owning group id: 9
status: status/Planning
bookmark: false
mutable description: A copper iduceble promoter with RFP for detection
mutable notes: ---
mutable provenance: CopAp is a copper induceble promoter out of ``E.coli`` K12
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: mRFP
sequence annotation: CopAP
sequence: https://synbiohub.org/public/igem/BBa_K1758321_sequence/1

mRFP
type: SequenceAnnotation
persistent identity: annotation2440067
display id: annotation2440067
version: 1
top level: BBa_K1758321
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1758321/annotation2440067/range2440067/1

https://synbiohub.org/public/igem/BBa_K1758321/annotation2440067/range2440067/1
type: Range
persistent identity: range2440067
display id: range2440067
version: 1
direction: direction/forward
top level: BBa_K1758321
start: 90
end: 770
orientation: inline

CopAP
type: SequenceAnnotation
persistent identity: annotation2440066
display id: annotation2440066
version: 1
top level: BBa_K1758321
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1758321/annotation2440066/range2440066/1

https://synbiohub.org/public/igem/BBa_K1758321/annotation2440066/range2440066/1
type: Range
persistent identity: range2440066
display id: range2440066
version: 1
direction: direction/forward
top level: BBa_K1758321
start: 1
end: 83
orientation: inline

BBa_K1689013
type: ComponentDefinition
persistent identity: BBa K1689013
display id: BBa_K1689013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Nlact-dCas9
creator: Jiaofeng Li
created: 2015-09-15T11:00:00Z
modified: 2015-09-27T01:29:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2107_
m user id: 26531
owner id: 26434
owning group id: 9
status: status/Planning
bookmark: false
mutable description: NFluc of lactamase fused with dCas9, His tag allows for protein purification
mutable notes: goldengate
mutable provenance: E.coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: Nlact-dCas9
sequence annotation: Nlact for N terminal of β-Lactamase
sequence annotation: GGGGS*2 Linker
sequence annotation: start
sequence annotation: random spacer
sequence annotation: dCas9
sequence annotation: His
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_K1689013_sequence/1

Nlact-dCas9
type: SequenceAnnotation
persistent identity: annotation2470114
display id: annotation2470114
version: 1
top level: BBa_K1689013
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1689013/annotation2470114/range2470114/1

https://synbiohub.org/public/igem/BBa_K1689013/annotation2470114/range2470114/1
type: Range
persistent identity: range2470114
display id: range2470114
version: 1
direction: direction/forward
top level: BBa_K1689013
start: 34
end: 4701
orientation: inline

Nlact for N terminal of β-Lactamase
type: SequenceAnnotation
persistent identity: annotation2470116
display id: annotation2470116
version: 1
top level: BBa_K1689013
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1689013/annotation2470116/range2470116/1

https://synbiohub.org/public/igem/BBa_K1689013/annotation2470116/range2470116/1
type: Range
persistent identity: range2470116
display id: range2470116
version: 1
direction: direction/forward
top level: BBa_K1689013
start: 55
end: 567
orientation: inline

GGGGS*2 Linker
type: SequenceAnnotation
persistent identity: annotation2470117
display id: annotation2470117
version: 1
top level: BBa_K1689013
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1689013/annotation2470117/range2470117/1

https://synbiohub.org/public/igem/BBa_K1689013/annotation2470117/range2470117/1
type: Range
persistent identity: range2470117
display id: range2470117
version: 1
direction: direction/forward
top level: BBa_K1689013
start: 568
end: 697
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2477773
display id: annotation2477773
version: 1
top level: BBa_K1689013
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1689013/annotation2477773/range2477773/1

https://synbiohub.org/public/igem/BBa_K1689013/annotation2477773/range2477773/1
type: Range
persistent identity: range2477773
display id: range2477773
version: 1
direction: direction/forward
top level: BBa_K1689013
start: 34
end: 36
orientation: inline

random spacer
type: SequenceAnnotation
persistent identity: annotation2477774
display id: annotation2477774
version: 1
top level: BBa_K1689013
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1689013/annotation2477774/range2477774/1

https://synbiohub.org/public/igem/BBa_K1689013/annotation2477774/range2477774/1
type: Range
persistent identity: range2477774
display id: range2477774
version: 1
direction: direction/forward
top level: BBa_K1689013
start: 1
end: 33
orientation: inline

dCas9
type: SequenceAnnotation
persistent identity: annotation2470119
display id: annotation2470119
version: 1
top level: BBa_K1689013
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1689013/annotation2470119/range2470119/1

https://synbiohub.org/public/igem/BBa_K1689013/annotation2470119/range2470119/1
type: Range
persistent identity: range2470119
display id: range2470119
version: 1
direction: direction/forward
top level: BBa_K1689013
start: 598
end: 4698
orientation: inline

His
type: SequenceAnnotation
persistent identity: annotation2470115
display id: annotation2470115
version: 1
top level: BBa_K1689013
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1689013/annotation2470115/range2470115/1

https://synbiohub.org/public/igem/BBa_K1689013/annotation2470115/range2470115/1
type: Range
persistent identity: range2470115
display id: range2470115
version: 1
direction: direction/forward
top level: BBa_K1689013
start: 37
end: 54
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2477776
display id: annotation2477776
version: 1
top level: BBa_K1689013
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1689013/annotation2477776/range2477776/1

https://synbiohub.org/public/igem/BBa_K1689013/annotation2477776/range2477776/1
type: Range
persistent identity: range2477776
display id: range2477776
version: 1
direction: direction/forward
top level: BBa_K1689013
start: 4699
end: 4701
orientation: inline

random spacer
type: SequenceAnnotation
persistent identity: annotation2477775
display id: annotation2477775
version: 1
top level: BBa_K1689013
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1689013/annotation2477775/range2477775/1

https://synbiohub.org/public/igem/BBa_K1689013/annotation2477775/range2477775/1
type: Range
persistent identity: range2477775
display id: range2477775
version: 1
direction: direction/forward
top level: BBa_K1689013
start: 4701
end: 4772
orientation: inline

BBa_K1799012
type: ComponentDefinition
persistent identity: BBa K1799012
display id: BBa_K1799012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LsrC RBS
creator: Will Shindel
created: 2015-08-26T11:00:00Z
modified: 2015-08-27T03:09:30Z
discontinued: false
dominant: false
group u list: _2224_
m user id: 26954
owner id: 26954
owning group id: 9
status: status/Planning
bookmark: false
mutable description: RBS located upstream of the LsrC open reading frame (inside the LsrA open reading frame) in E. coli
mutable notes: None
mutable provenance: This part was synthesized, and is based on E. coli genomic data retrieved via EcoGene: http://www.ecogene.org/gene/EG13809
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_K1799012_sequence/1

BBa_K1758361
type: ComponentDefinition
persistent identity: BBa K1758361
display id: BBa_K1758361
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: mRFP1 controlled by the GABA-inducible activator GabR
creator: Team Bielefeld-CeBiTec 2015
created: 2015-08-21T11:00:00Z
modified: 2015-09-18T02:48:20Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2180_
m user id: 26913
owner id: 26587
owning group id: 9
status: status/Planning
bookmark: true
mutable description: gabR encodes a repressor which controls the expression of mRFP1.
mutable notes: This device was created by 3A assembly and subsequent subcloning into pSB1C3.
mutable provenance: BBa_K1758350 was obtained by gene synthesis. BBa_K516030 was part of the distribution.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K516030
sequence annotation: BBa_K516030
sequence: https://synbiohub.org/public/igem/BBa_K1758361_sequence/1

BBa_K516030
type: Component
persistent identity: component2436864
display id: component2436864
version: 1
top level: BBa_K1758361
access: public
definition: https://synbiohub.org/public/igem/BBa_K516030/1

BBa_K516030
type: SequenceAnnotation
persistent identity: annotation2436864
display id: annotation2436864
version: 1
top level: BBa_K1758361
location: https://synbiohub.org/public/igem/BBa_K1758361/annotation2436864/range2436864/1
component: BBa_K516030

https://synbiohub.org/public/igem/BBa_K1758361/annotation2436864/range2436864/1
type: Range
persistent identity: range2436864
display id: range2436864
version: 1
direction: direction/reverse
top level: BBa_K1758361
start: 1555
end: 2418
orientation: inline

BBa_K1758312
type: ComponentDefinition
persistent identity: BBa K1758312
display id: BBa_K1758312
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Chromium responsive promoter with UTR+RBS and sfGFP
creator: Team Bielefeld-CeBiTec 2015
created: 2015-08-28T11:00:00Z
modified: 2016-01-06T03:35:44Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2180_
m user id: 4206
owner id: 26630
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Chromium responsive promoter with 5?? untremslated region and sfGFP for detection
mutable notes: ...
mutable provenance: Biobrick modified after K1058007
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: 5-UTR
sequence annotation: T1
sequence annotation: sfGFP
sequence annotation: T7-term
sequence annotation: Cr-promoter
sequence: https://synbiohub.org/public/igem/BBa_K1758312_sequence/1

5-UTR
type: SequenceAnnotation
persistent identity: annotation2439957
display id: annotation2439957
version: 1
top level: BBa_K1758312
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1758312/annotation2439957/range2439957/1

https://synbiohub.org/public/igem/BBa_K1758312/annotation2439957/range2439957/1
type: Range
persistent identity: range2439957
display id: range2439957
version: 1
direction: direction/forward
top level: BBa_K1758312
start: 171
end: 216
orientation: inline

T1
type: SequenceAnnotation
persistent identity: annotation2439959
display id: annotation2439959
version: 1
top level: BBa_K1758312
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1758312/annotation2439959/range2439959/1

https://synbiohub.org/public/igem/BBa_K1758312/annotation2439959/range2439959/1
type: Range
persistent identity: range2439959
display id: range2439959
version: 1
direction: direction/forward
top level: BBa_K1758312
start: 945
end: 1024
orientation: inline

sfGFP
type: SequenceAnnotation
persistent identity: annotation2439958
display id: annotation2439958
version: 1
top level: BBa_K1758312
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1758312/annotation2439958/range2439958/1

https://synbiohub.org/public/igem/BBa_K1758312/annotation2439958/range2439958/1
type: Range
persistent identity: range2439958
display id: range2439958
version: 1
direction: direction/forward
top level: BBa_K1758312
start: 217
end: 936
orientation: inline

T7-term
type: SequenceAnnotation
persistent identity: annotation2439960
display id: annotation2439960
version: 1
top level: BBa_K1758312
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1758312/annotation2439960/range2439960/1

https://synbiohub.org/public/igem/BBa_K1758312/annotation2439960/range2439960/1
type: Range
persistent identity: range2439960
display id: range2439960
version: 1
direction: direction/forward
top level: BBa_K1758312
start: 1033
end: 1073
orientation: inline

Cr-promoter
type: SequenceAnnotation
persistent identity: annotation2439956
display id: annotation2439956
version: 1
top level: BBa_K1758312
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1758312/annotation2439956/range2439956/1

https://synbiohub.org/public/igem/BBa_K1758312/annotation2439956/range2439956/1
type: Range
persistent identity: range2439956
display id: range2439956
version: 1
direction: direction/forward
top level: BBa_K1758312
start: 1
end: 164
orientation: inline

mLacI
type: ComponentDefinition
persistent identity: BBa K1639011
display id: BBa_K1639011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: mammalian LacI with miR-223 and miR-21 binding sites
creator: Nurgeldi Bazarov
created: 2015-09-14T11:00:00Z
modified: 2015-09-15T12:46:07Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2056_
m user id: 24789
owner id: 24789
owning group id: 9
status: status/Planning
bookmark: false
mutable description: In our project second step of cancer module is based on production of LacI so that it will suppress expression of DsRed. Binding site on 3' end of LacI makes it regulatable and limits it's expression only in cells with low miR-223 and miR-21 levels. miR-21 has high rate of production in most cancer types. In gastric epithelial cells level of this miRNAs are low but in gastric cancer cells their level is very high.
mutable notes: We suggest also to view similar part we submitted-BBa_K1639010. It contains mLacI with miR binding sites but have restriction enzyme cut sites to remove miR binding site as needed.
mutable provenance: This protein is mammalian codon optimized version of LacI repressor originally derived from E.coli. It also possess nuclear localization signal (NLS) so that it can act on lac operators in nucleus.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: miR-223 binding site
sequence annotation: SV40 NLS
sequence annotation: Kozak
sequence annotation: mLacI
sequence annotation: Stop Codon
sequence annotation: ATG
sequence annotation: miR-21 binding site
sequence: https://synbiohub.org/public/igem/BBa_K1639011_sequence/1

miR-223 binding site
type: SequenceAnnotation
persistent identity: annotation2457428
display id: annotation2457428
version: 1
top level: mLacI
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1639011/annotation2457428/range2457428/1

https://synbiohub.org/public/igem/BBa_K1639011/annotation2457428/range2457428/1
type: Range
persistent identity: range2457428
display id: range2457428
version: 1
direction: direction/forward
top level: mLacI
start: 1129
end: 1150
orientation: inline

SV40 NLS
type: SequenceAnnotation
persistent identity: annotation2457422
display id: annotation2457422
version: 1
top level: mLacI
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1639011/annotation2457422/range2457422/1

https://synbiohub.org/public/igem/BBa_K1639011/annotation2457422/range2457422/1
type: Range
persistent identity: range2457422
display id: range2457422
version: 1
direction: direction/forward
top level: mLacI
start: 1102
end: 1122
orientation: inline

Kozak
type: SequenceAnnotation
persistent identity: annotation2457408
display id: annotation2457408
version: 1
top level: mLacI
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1639011/annotation2457408/range2457408/1

https://synbiohub.org/public/igem/BBa_K1639011/annotation2457408/range2457408/1
type: Range
persistent identity: range2457408
display id: range2457408
version: 1
direction: direction/forward
top level: mLacI
start: 1
end: 6
orientation: inline

mLacI
type: SequenceAnnotation
persistent identity: annotation2457411
display id: annotation2457411
version: 1
top level: mLacI
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1639011/annotation2457411/range2457411/1

https://synbiohub.org/public/igem/BBa_K1639011/annotation2457411/range2457411/1
type: Range
persistent identity: range2457411
display id: range2457411
version: 1
direction: direction/forward
top level: mLacI
start: 7
end: 1125
orientation: inline

Stop Codon
type: SequenceAnnotation
persistent identity: annotation2457427
display id: annotation2457427
version: 1
top level: mLacI
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1639011/annotation2457427/range2457427/1

https://synbiohub.org/public/igem/BBa_K1639011/annotation2457427/range2457427/1
type: Range
persistent identity: range2457427
display id: range2457427
version: 1
direction: direction/forward
top level: mLacI
start: 1123
end: 1128
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2457409
display id: annotation2457409
version: 1
top level: mLacI
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1639011/annotation2457409/range2457409/1

https://synbiohub.org/public/igem/BBa_K1639011/annotation2457409/range2457409/1
type: Range
persistent identity: range2457409
display id: range2457409
version: 1
direction: direction/forward
top level: mLacI
start: 7
end: 9
orientation: inline

miR-21 binding site
type: SequenceAnnotation
persistent identity: annotation2457429
display id: annotation2457429
version: 1
top level: mLacI
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1639011/annotation2457429/range2457429/1

https://synbiohub.org/public/igem/BBa_K1639011/annotation2457429/range2457429/1
type: Range
persistent identity: range2457429
display id: range2457429
version: 1
direction: direction/forward
top level: mLacI
start: 1180
end: 1200
orientation: inline

BBa_K1850011
type: ComponentDefinition
persistent identity: BBa K1850011
display id: BBa_K1850011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pRha - fimH 49 KO - RPMrel - SpyTag_225 - HisTag_225
creator: Lydia Goldberg
created: 2015-09-14T11:00:00Z
modified: 2015-09-16T10:48:20Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2277_
m user id: 26421
owner id: 26421
owning group id: 9
status: status/Planning
bookmark: false
mutable description: placeholder
mutable notes: placeholder
mutable provenance: placeholder
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: pRha
sequence annotation: SpyTag
sequence annotation: HisTag
sequence annotation: RPMrel
sequence annotation: fimH
sequence annotation: B0034
sequence: https://synbiohub.org/public/igem/BBa_K1850011_sequence/1

pRha
type: SequenceAnnotation
persistent identity: annotation2463792
display id: annotation2463792
version: 1
top level: BBa_K1850011
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1850011/annotation2463792/range2463792/1

https://synbiohub.org/public/igem/BBa_K1850011/annotation2463792/range2463792/1
type: Range
persistent identity: range2463792
display id: range2463792
version: 1
direction: direction/forward
top level: BBa_K1850011
start: 4
end: 243
orientation: inline

SpyTag
type: SequenceAnnotation
persistent identity: annotation2463798
display id: annotation2463798
version: 1
top level: BBa_K1850011
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1850011/annotation2463798/range2463798/1

https://synbiohub.org/public/igem/BBa_K1850011/annotation2463798/range2463798/1
type: Range
persistent identity: range2463798
display id: range2463798
version: 1
direction: direction/forward
top level: BBa_K1850011
start: 1032
end: 1070
orientation: inline

HisTag
type: SequenceAnnotation
persistent identity: annotation2463799
display id: annotation2463799
version: 1
top level: BBa_K1850011
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1850011/annotation2463799/range2463799/1

https://synbiohub.org/public/igem/BBa_K1850011/annotation2463799/range2463799/1
type: Range
persistent identity: range2463799
display id: range2463799
version: 1
direction: direction/forward
top level: BBa_K1850011
start: 1071
end: 1088
orientation: inline

RPMrel
type: SequenceAnnotation
persistent identity: annotation2463796
display id: annotation2463796
version: 1
top level: BBa_K1850011
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1850011/annotation2463796/range2463796/1

https://synbiohub.org/public/igem/BBa_K1850011/annotation2463796/range2463796/1
type: Range
persistent identity: range2463796
display id: range2463796
version: 1
direction: direction/forward
top level: BBa_K1850011
start: 333
end: 359
orientation: inline

fimH
type: SequenceAnnotation
persistent identity: annotation2463795
display id: annotation2463795
version: 1
top level: BBa_K1850011
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1850011/annotation2463795/range2463795/1

https://synbiohub.org/public/igem/BBa_K1850011/annotation2463795/range2463795/1
type: Range
persistent identity: range2463795
display id: range2463795
version: 1
direction: direction/forward
top level: BBa_K1850011
start: 270
end: 1256
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2463794
display id: annotation2463794
version: 1
top level: BBa_K1850011
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1850011/annotation2463794/range2463794/1

https://synbiohub.org/public/igem/BBa_K1850011/annotation2463794/range2463794/1
type: Range
persistent identity: range2463794
display id: range2463794
version: 1
direction: direction/forward
top level: BBa_K1850011
start: 252
end: 263
orientation: inline

BBa_K1689018
type: ComponentDefinition
persistent identity: BBa K1689018
display id: BBa_K1689018
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: dCas9-F3
creator: Wei weijia
created: 2015-09-15T11:00:00Z
modified: 2015-09-27T09:51:28Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2107_
m user id: 26531
owner id: 26531
owning group id: 9
status: status/Planning
bookmark: false
mutable description: pSB1C3-6H-dCas9-F3, this is the fusion protein of dCas9-F3, His tag allows for protein purification
mutable notes: The coding sequence of this fusion protein is too long, you'd better amplify is separately.
mutable provenance: E.coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: start
sequence annotation: random spacer
sequence annotation: His
sequence annotation: dCas9-F3
sequence annotation: GGGGS*2 Linker
sequence annotation: stop
sequence annotation: F3
sequence annotation: dCas9
sequence: https://synbiohub.org/public/igem/BBa_K1689018_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation2477794
display id: annotation2477794
version: 1
top level: BBa_K1689018
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1689018/annotation2477794/range2477794/1

https://synbiohub.org/public/igem/BBa_K1689018/annotation2477794/range2477794/1
type: Range
persistent identity: range2477794
display id: range2477794
version: 1
direction: direction/forward
top level: BBa_K1689018
start: 34
end: 36
orientation: inline

random spacer
type: SequenceAnnotation
persistent identity: annotation2477796
display id: annotation2477796
version: 1
top level: BBa_K1689018
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1689018/annotation2477796/range2477796/1

https://synbiohub.org/public/igem/BBa_K1689018/annotation2477796/range2477796/1
type: Range
persistent identity: range2477796
display id: range2477796
version: 1
direction: direction/forward
top level: BBa_K1689018
start: 4402
end: 4472
orientation: inline

His
type: SequenceAnnotation
persistent identity: annotation2469989
display id: annotation2469989
version: 1
top level: BBa_K1689018
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1689018/annotation2469989/range2469989/1

https://synbiohub.org/public/igem/BBa_K1689018/annotation2469989/range2469989/1
type: Range
persistent identity: range2469989
display id: range2469989
version: 1
direction: direction/forward
top level: BBa_K1689018
start: 37
end: 54
orientation: inline

dCas9-F3
type: SequenceAnnotation
persistent identity: annotation2469985
display id: annotation2469985
version: 1
top level: BBa_K1689018
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1689018/annotation2469985/range2469985/1

https://synbiohub.org/public/igem/BBa_K1689018/annotation2469985/range2469985/1
type: Range
persistent identity: range2469985
display id: range2469985
version: 1
direction: direction/forward
top level: BBa_K1689018
start: 34
end: 4401
orientation: inline

GGGGS*2 Linker
type: SequenceAnnotation
persistent identity: annotation2469994
display id: annotation2469994
version: 1
top level: BBa_K1689018
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1689018/annotation2469994/range2469994/1

https://synbiohub.org/public/igem/BBa_K1689018/annotation2469994/range2469994/1
type: Range
persistent identity: range2469994
display id: range2469994
version: 1
direction: direction/forward
top level: BBa_K1689018
start: 4156
end: 4185
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2477795
display id: annotation2477795
version: 1
top level: BBa_K1689018
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1689018/annotation2477795/range2477795/1

https://synbiohub.org/public/igem/BBa_K1689018/annotation2477795/range2477795/1
type: Range
persistent identity: range2477795
display id: range2477795
version: 1
direction: direction/forward
top level: BBa_K1689018
start: 4399
end: 4401
orientation: inline

F3
type: SequenceAnnotation
persistent identity: annotation2469996
display id: annotation2469996
version: 1
top level: BBa_K1689018
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1689018/annotation2469996/range2469996/1

https://synbiohub.org/public/igem/BBa_K1689018/annotation2469996/range2469996/1
type: Range
persistent identity: range2469996
display id: range2469996
version: 1
direction: direction/forward
top level: BBa_K1689018
start: 4186
end: 4398
orientation: inline

random spacer
type: SequenceAnnotation
persistent identity: annotation2477793
display id: annotation2477793
version: 1
top level: BBa_K1689018
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1689018/annotation2477793/range2477793/1

https://synbiohub.org/public/igem/BBa_K1689018/annotation2477793/range2477793/1
type: Range
persistent identity: range2477793
display id: range2477793
version: 1
direction: direction/forward
top level: BBa_K1689018
start: 1
end: 33
orientation: inline

dCas9
type: SequenceAnnotation
persistent identity: annotation2469992
display id: annotation2469992
version: 1
top level: BBa_K1689018
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1689018/annotation2469992/range2469992/1

https://synbiohub.org/public/igem/BBa_K1689018/annotation2469992/range2469992/1
type: Range
persistent identity: range2469992
display id: range2469992
version: 1
direction: direction/forward
top level: BBa_K1689018
start: 55
end: 4155
orientation: inline

BBa_K1758331
type: ComponentDefinition
persistent identity: BBa K1758331
display id: BBa_K1758331
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lead responsive promoter with mRFP
creator: Team Bielefeld-CeBiTec 2015
created: 2015-08-29T11:00:00Z
modified: 2016-01-06T03:42:01Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2180_
m user id: 4206
owner id: 26630
owning group id: 9
status: status/Planning
bookmark: false
mutable description: lead responsive promoter with mRFP for detection
mutable notes: ...
mutable provenance: lead responsive promoter of ????Cupriavidus metallidurans???? CH34
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: pbrA
sequence annotation: mRFP
sequence: https://synbiohub.org/public/igem/BBa_K1758331_sequence/1

pbrA
type: SequenceAnnotation
persistent identity: annotation2440072
display id: annotation2440072
version: 1
top level: BBa_K1758331
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1758331/annotation2440072/range2440072/1

https://synbiohub.org/public/igem/BBa_K1758331/annotation2440072/range2440072/1
type: Range
persistent identity: range2440072
display id: range2440072
version: 1
direction: direction/forward
top level: BBa_K1758331
start: 1
end: 68
orientation: inline

mRFP
type: SequenceAnnotation
persistent identity: annotation2440073
display id: annotation2440073
version: 1
top level: BBa_K1758331
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1758331/annotation2440073/range2440073/1

https://synbiohub.org/public/igem/BBa_K1758331/annotation2440073/range2440073/1
type: Range
persistent identity: range2440073
display id: range2440073
version: 1
direction: direction/forward
top level: BBa_K1758331
start: 75
end: 755
orientation: inline

Yeb-Art175
type: ComponentDefinition
persistent identity: BBa K1659003
display id: BBa_K1659003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Artilysin Art-175 fused at N-terminal with porin-dependent YebF secretion signal protein
creator: Wei Chung Kong
created: 2015-08-27T11:00:00Z
modified: 2016-02-01T12:52:17Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2077_
m user id: 4206
owner id: 24524
owning group id: 9
status: status/Planning
bookmark: false
mutable description: to be updated
mutable notes: to be updated
mutable provenance: to be updated
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: start
sequence annotation: YebF
sequence annotation: Art-175
sequence annotation: stop
sequence annotation: Hisx6
sequence: https://synbiohub.org/public/igem/BBa_K1659003_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation2445825
display id: annotation2445825
version: 1
top level: Yeb-Art175
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1659003/annotation2445825/range2445825/1

https://synbiohub.org/public/igem/BBa_K1659003/annotation2445825/range2445825/1
type: Range
persistent identity: range2445825
display id: range2445825
version: 1
direction: direction/forward
top level: Yeb-Art175
start: 1
end: 3
orientation: inline

YebF
type: SequenceAnnotation
persistent identity: annotation2445826
display id: annotation2445826
version: 1
top level: Yeb-Art175
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1659003/annotation2445826/range2445826/1

https://synbiohub.org/public/igem/BBa_K1659003/annotation2445826/range2445826/1
type: Range
persistent identity: range2445826
display id: range2445826
version: 1
direction: direction/forward
top level: Yeb-Art175
start: 4
end: 354
orientation: inline

Art-175
type: SequenceAnnotation
persistent identity: annotation2445827
display id: annotation2445827
version: 1
top level: Yeb-Art175
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1659003/annotation2445827/range2445827/1

https://synbiohub.org/public/igem/BBa_K1659003/annotation2445827/range2445827/1
type: Range
persistent identity: range2445827
display id: range2445827
version: 1
direction: direction/forward
top level: Yeb-Art175
start: 355
end: 1221
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2445829
display id: annotation2445829
version: 1
top level: Yeb-Art175
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1659003/annotation2445829/range2445829/1

https://synbiohub.org/public/igem/BBa_K1659003/annotation2445829/range2445829/1
type: Range
persistent identity: range2445829
display id: range2445829
version: 1
direction: direction/forward
top level: Yeb-Art175
start: 1240
end: 1245
orientation: inline

Hisx6
type: SequenceAnnotation
persistent identity: annotation2445828
display id: annotation2445828
version: 1
top level: Yeb-Art175
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1659003/annotation2445828/range2445828/1

https://synbiohub.org/public/igem/BBa_K1659003/annotation2445828/range2445828/1
type: Range
persistent identity: range2445828
display id: range2445828
version: 1
direction: direction/forward
top level: Yeb-Art175
start: 1222
end: 1239
orientation: inline

BBa_K1789006
type: ComponentDefinition
persistent identity: BBa K1789006
display id: BBa_K1789006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SCAF2
creator: Xinyuan Qiu
created: 2015-09-12T11:00:00Z
modified: 2015-09-18T08:33:02Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2214_
m user id: 21134
owner id: 26299
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This part is a highly repetitive DNA sequence which can bind TALE protein specifically.
mutable notes: This part is designed to contain two basic short sequences which can bind TALE1 and TALE2(3) specifically. These two basic sequences are repeated with no space by ten times, so it can bind more TALE protein and the spatial location of each pair of TALE1 and TALE2(3) is almost opposite.
mutable provenance: This part is synthetized by viewsolid biotech firm (Beijing, China).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: BM2
sequence annotation: SCAF2
sequence annotation: BM1
sequence: https://synbiohub.org/public/igem/BBa_K1789006_sequence/1

BM2
type: SequenceAnnotation
persistent identity: annotation2470918
display id: annotation2470918
version: 1
top level: BBa_K1789006
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1789006/annotation2470918/range2470918/1

https://synbiohub.org/public/igem/BBa_K1789006/annotation2470918/range2470918/1
type: Range
persistent identity: range2470918
display id: range2470918
version: 1
direction: direction/forward
top level: BBa_K1789006
start: 21
end: 34
orientation: inline

SCAF2
type: SequenceAnnotation
persistent identity: annotation2453186
display id: annotation2453186
version: 1
top level: BBa_K1789006
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1789006/annotation2453186/range2453186/1

https://synbiohub.org/public/igem/BBa_K1789006/annotation2453186/range2453186/1
type: Range
persistent identity: range2453186
display id: range2453186
version: 1
direction: direction/forward
top level: BBa_K1789006
start: 1
end: 462
orientation: inline

BM1
type: SequenceAnnotation
persistent identity: annotation2470907
display id: annotation2470907
version: 1
top level: BBa_K1789006
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1789006/annotation2470907/range2470907/1

https://synbiohub.org/public/igem/BBa_K1789006/annotation2470907/range2470907/1
type: Range
persistent identity: range2470907
display id: range2470907
version: 1
direction: direction/forward
top level: BBa_K1789006
start: 1
end: 14
orientation: inline

BBa_I20270
type: ComponentDefinition
persistent identity: BBa I20270
display id: BBa_I20270
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter MeasKit (J23151)
creator: Jason Kelly
created: 2008-02-06T12:00:00Z
modified: 2015-08-31T04:07:39Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 2
owning group id: 84
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: J23108 inserted in the Promoter MeasKit
mutable notes: .
mutable provenance: .
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_B0012
component: BBa_E0040
component: BBa_B0032
component: BBa_J23151
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0032
sequence annotation: BBa_J23151
sequence annotation: BBa_B0012
sequence annotation: BBa_E0040
sequence: https://synbiohub.org/public/igem/BBa_I20270_sequence/1

BBa_B0012
type: Component
persistent identity: component1961207
display id: component1961207
version: 1
top level: BBa_I20270
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_E0040
type: Component
persistent identity: component1961204
display id: component1961204
version: 1
top level: BBa_I20270
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_B0032
type: Component
persistent identity: component1961201
display id: component1961201
version: 1
top level: BBa_I20270
access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_J23151
type: Component
persistent identity: component1961199
display id: component1961199
version: 1
top level: BBa_I20270
access: public
definition: https://synbiohub.org/public/igem/BBa_J23151/1

BBa_B0010
type: Component
persistent identity: component1961205
display id: component1961205
version: 1
top level: BBa_I20270
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1961205
display id: annotation1961205
version: 1
top level: BBa_I20270
location: https://synbiohub.org/public/igem/BBa_I20270/annotation1961205/range1961205/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I20270/annotation1961205/range1961205/1
type: Range
persistent identity: range1961205
display id: range1961205
version: 1
direction: direction/forward
top level: BBa_I20270
start: 791
end: 870
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1961201
display id: annotation1961201
version: 1
top level: BBa_I20270
location: https://synbiohub.org/public/igem/BBa_I20270/annotation1961201/range1961201/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_I20270/annotation1961201/range1961201/1
type: Range
persistent identity: range1961201
display id: range1961201
version: 1
direction: direction/forward
top level: BBa_I20270
start: 44
end: 56
orientation: inline

BBa_J23151
type: SequenceAnnotation
persistent identity: annotation1961199
display id: annotation1961199
version: 1
top level: BBa_I20270
location: https://synbiohub.org/public/igem/BBa_I20270/annotation1961199/range1961199/1
component: BBa_J23151

https://synbiohub.org/public/igem/BBa_I20270/annotation1961199/range1961199/1
type: Range
persistent identity: range1961199
display id: range1961199
version: 1
direction: direction/reverse
top level: BBa_I20270
start: 1
end: 35
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1961207
display id: annotation1961207
version: 1
top level: BBa_I20270
location: https://synbiohub.org/public/igem/BBa_I20270/annotation1961207/range1961207/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I20270/annotation1961207/range1961207/1
type: Range
persistent identity: range1961207
display id: range1961207
version: 1
direction: direction/forward
top level: BBa_I20270
start: 879
end: 919
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1961204
display id: annotation1961204
version: 1
top level: BBa_I20270
location: https://synbiohub.org/public/igem/BBa_I20270/annotation1961204/range1961204/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_I20270/annotation1961204/range1961204/1
type: Range
persistent identity: range1961204
display id: range1961204
version: 1
direction: direction/reverse
top level: BBa_I20270
start: 63
end: 782
orientation: inline

BBa_K1692032
type: ComponentDefinition
persistent identity: BBa K1692032
display id: BBa_K1692032
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: amilCP blue chromoprotein with RBS and promoter
creator: Jack Ryan Takahashi
created: 2015-09-13T11:00:00Z
modified: 2015-09-18T05:00:27Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2110_
m user id: 26427
owner id: 26427
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This plasmid contains the amilCP blue chromoprotein gene found in part BBa_K592009 and the RBS and promoter from part BBa_K608002.
mutable notes: N/A
mutable provenance: This part is made from parts BBa_K608002 and BBa_K592009.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_K592009
component: BBa_K608002
sequence annotation: BBa_K592009
sequence annotation: BBa_K608002
sequence: https://synbiohub.org/public/igem/BBa_K1692032_sequence/1

BBa_K592009
type: Component
persistent identity: component2455198
display id: component2455198
version: 1
top level: BBa_K1692032
access: public
definition: https://synbiohub.org/public/igem/BBa_K592009/1

BBa_K608002
type: Component
persistent identity: component2455196
display id: component2455196
version: 1
top level: BBa_K1692032
access: public
definition: https://synbiohub.org/public/igem/BBa_K608002/1

BBa_K592009
type: SequenceAnnotation
persistent identity: annotation2455198
display id: annotation2455198
version: 1
top level: BBa_K1692032
location: https://synbiohub.org/public/igem/BBa_K1692032/annotation2455198/range2455198/1
component: BBa_K592009

https://synbiohub.org/public/igem/BBa_K1692032/annotation2455198/range2455198/1
type: Range
persistent identity: range2455198
display id: range2455198
version: 1
direction: direction/reverse
top level: BBa_K1692032
start: 62
end: 730
orientation: inline

BBa_K608002
type: SequenceAnnotation
persistent identity: annotation2455196
display id: annotation2455196
version: 1
top level: BBa_K1692032
location: https://synbiohub.org/public/igem/BBa_K1692032/annotation2455196/range2455196/1
component: BBa_K608002

https://synbiohub.org/public/igem/BBa_K1692032/annotation2455196/range2455196/1
type: Range
persistent identity: range2455196
display id: range2455196
version: 1
direction: direction/reverse
top level: BBa_K1692032
start: 1
end: 55
orientation: inline

BBa_K1632013
type: ComponentDefinition
persistent identity: BBa K1632013
display id: BBa_K1632013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PBAD/araC_rbs_fimE(wild-type)
creator: Riku Shinohara
created: 2015-08-29T11:00:00Z
modified: 2015-09-15T07:47:30Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2049_
m user id: 24760
owner id: 22687
owning group id: 9
status: status/Planning
bookmark: false
mutable description: a
mutable notes: a
mutable provenance: a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0034
component: BBa_I0500
component: BBa_K1632011
sequence annotation: BBa_I0500
sequence annotation: BBa_B0034
sequence annotation: BBa_K1632011
sequence: https://synbiohub.org/public/igem/BBa_K1632013_sequence/1

BBa_B0034
type: Component
persistent identity: component2440382
display id: component2440382
version: 1
top level: BBa_K1632013
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_I0500
type: Component
persistent identity: component2440380
display id: component2440380
version: 1
top level: BBa_K1632013
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_K1632011
type: Component
persistent identity: component2440383
display id: component2440383
version: 1
top level: BBa_K1632013
access: public
definition: BBa_K1632011

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2440380
display id: annotation2440380
version: 1
top level: BBa_K1632013
location: https://synbiohub.org/public/igem/BBa_K1632013/annotation2440380/range2440380/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_K1632013/annotation2440380/range2440380/1
type: Range
persistent identity: range2440380
display id: range2440380
version: 1
direction: direction/reverse
top level: BBa_K1632013
start: 1
end: 1210
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2440382
display id: annotation2440382
version: 1
top level: BBa_K1632013
location: https://synbiohub.org/public/igem/BBa_K1632013/annotation2440382/range2440382/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1632013/annotation2440382/range2440382/1
type: Range
persistent identity: range2440382
display id: range2440382
version: 1
direction: direction/reverse
top level: BBa_K1632013
start: 1219
end: 1230
orientation: inline

BBa_K1632011
type: SequenceAnnotation
persistent identity: annotation2440383
display id: annotation2440383
version: 1
top level: BBa_K1632013
location: https://synbiohub.org/public/igem/BBa_K1632013/annotation2440383/range2440383/1
component: BBa_K1632011

https://synbiohub.org/public/igem/BBa_K1632013/annotation2440383/range2440383/1
type: Range
persistent identity: range2440383
display id: range2440383
version: 1
direction: direction/reverse
top level: BBa_K1632013
start: 1239
end: 1835
orientation: inline

BBa_K1795002
type: ComponentDefinition
persistent identity: BBa K1795002
display id: BBa_K1795002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: R0010 gRNA under R0040
creator: Andrew D Halleran, Joseph L Maniaci
created: 2015-09-16T11:00:00Z
modified: 2015-09-17T09:40:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2220_
m user id: 27446
owner id: 27446
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This sgRNA sequence was designed to target the Promoter Bba_R0010 with the N20 sequence atgttgtgtggaattgtgag . If this gRNA is expressed in a cell with a form of the dCas9 protein, the protein will be targeted to the N20 sequence and repress gene expression. The part is under the control of the promoter Bba_R0040. Promoter Bba_R0040 was chosen because it does not contain a PAM and therefore cannot be inadvertently repressed by dCas9 due to unintentional homology in the N20 sequence. Expression of this part can be repressed if TetR is expressed in the cell without Tetracycline present. If TetR and Tetracycline (or its analog aTc) are both present, the part will be expressed.
mutable notes: We made sure to not target the sequence that is promoting the gRNA
mutable provenance: The basic template for the sgRNA sequence was taken from the supplementary information of Qi LS, Larson MH, Gilbert LA, et al. Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression. Cell. 2013;152(5):1173-1183. doi:10.1016/j.cell.2013.02.022.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_R0040
component: BBa_K1795100
component: BBa_B0015
component: BBa_K1795102
sequence annotation: BBa_K1795102
sequence annotation: BBa_R0040
sequence annotation: BBa_B0015
sequence annotation: BBa_K1795100
sequence: https://synbiohub.org/public/igem/BBa_K1795002_sequence/1

BBa_R0040
type: Component
persistent identity: component2467679
display id: component2467679
version: 1
top level: BBa_K1795002
access: public
definition: p(tetR)

BBa_K1795100
type: Component
persistent identity: component2467685
display id: component2467685
version: 1
top level: BBa_K1795002
access: public
definition: https://synbiohub.org/public/igem/BBa_K1795100/1

BBa_B0015
type: Component
persistent identity: component2467692
display id: component2467692
version: 1
top level: BBa_K1795002
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_K1795102
type: Component
persistent identity: component2467684
display id: component2467684
version: 1
top level: BBa_K1795002
access: public
definition: https://synbiohub.org/public/igem/BBa_K1795102/1

BBa_K1795102
type: SequenceAnnotation
persistent identity: annotation2467684
display id: annotation2467684
version: 1
top level: BBa_K1795002
location: https://synbiohub.org/public/igem/BBa_K1795002/annotation2467684/range2467684/1
component: BBa_K1795102

https://synbiohub.org/public/igem/BBa_K1795002/annotation2467684/range2467684/1
type: Range
persistent identity: range2467684
display id: range2467684
version: 1
direction: direction/reverse
top level: BBa_K1795002
start: 55
end: 74
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2467679
display id: annotation2467679
version: 1
top level: BBa_K1795002
location: https://synbiohub.org/public/igem/BBa_K1795002/annotation2467679/range2467679/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K1795002/annotation2467679/range2467679/1
type: Range
persistent identity: range2467679
display id: range2467679
version: 1
direction: direction/forward
top level: BBa_K1795002
start: 1
end: 54
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2467692
display id: annotation2467692
version: 1
top level: BBa_K1795002
location: https://synbiohub.org/public/igem/BBa_K1795002/annotation2467692/range2467692/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1795002/annotation2467692/range2467692/1
type: Range
persistent identity: range2467692
display id: range2467692
version: 1
direction: direction/reverse
top level: BBa_K1795002
start: 157
end: 285
orientation: inline

BBa_K1795100
type: SequenceAnnotation
persistent identity: annotation2467685
display id: annotation2467685
version: 1
top level: BBa_K1795002
location: https://synbiohub.org/public/igem/BBa_K1795002/annotation2467685/range2467685/1
component: BBa_K1795100

https://synbiohub.org/public/igem/BBa_K1795002/annotation2467685/range2467685/1
type: Range
persistent identity: range2467685
display id: range2467685
version: 1
direction: direction/reverse
top level: BBa_K1795002
start: 75
end: 156
orientation: inline

BBa_K1602014
type: ComponentDefinition
persistent identity: BBa K1602014
display id: BBa_K1602014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0034-yagE
creator: Leon Kraus, Jonathan Pletzer-Zelgert, Jonas Sindlinger, Carmen Sakas Gandullo, Sebastian Holzner, Elena Tonchevska, Mirjam Haun
created: 2015-09-15T11:00:00Z
modified: 2015-09-18T07:26:18Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2019_
m user id: 25990
owner id: 22022
owning group id: 9
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_K1602011
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_K1602011
sequence: https://synbiohub.org/public/igem/BBa_K1602014_sequence/1

BBa_K1602011
type: Component
persistent identity: component2462504
display id: component2462504
version: 1
top level: BBa_K1602014
access: public
definition: BBa_K1602011

BBa_B0034
type: Component
persistent identity: component2462503
display id: component2462503
version: 1
top level: BBa_K1602014
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2462503
display id: annotation2462503
version: 1
top level: BBa_K1602014
location: https://synbiohub.org/public/igem/BBa_K1602014/annotation2462503/range2462503/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1602014/annotation2462503/range2462503/1
type: Range
persistent identity: range2462503
display id: range2462503
version: 1
direction: direction/reverse
top level: BBa_K1602014
start: 1
end: 12
orientation: inline

BBa_K1602011
type: SequenceAnnotation
persistent identity: annotation2462504
display id: annotation2462504
version: 1
top level: BBa_K1602014
location: https://synbiohub.org/public/igem/BBa_K1602014/annotation2462504/range2462504/1
component: BBa_K1602011

https://synbiohub.org/public/igem/BBa_K1602014/annotation2462504/range2462504/1
type: Range
persistent identity: range2462504
display id: range2462504
version: 1
direction: direction/reverse
top level: BBa_K1602014
start: 19
end: 927
orientation: inline

BBa_K1806001
type: ComponentDefinition
persistent identity: BBa K1806001
display id: BBa_K1806001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Helicobacter pylori catalase
creator: İBRAHİM YASİR ORHAN
created: 2015-09-15T11:00:00Z
modified: 2015-09-17T07:10:18Z
discontinued: false
dominant: false
group u list: _2231_
m user id: 11153
owner id: 11153
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This part is the coding sequence for the Helicobacter pylori catalase, which effectively catalyses the breakdown of hydrogen peroxide. The breakdown of hydrogen peroxide, which is an exothermic process, will be utilized to provide heat to the medium to increase temperature to counteract a shift that decreases the temperature of the host environment.
mutable notes: There are no specific design considerations that were made.
mutable provenance: Helicobacter pylori XZ274 http://www.ncbi.nlm.nih.gov/protein/AFJ81452.1 GenBank: AFJ81452.1
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1806001_sequence/1

BBa_K1847005
type: ComponentDefinition
persistent identity: BBa K1847005
display id: BBa_K1847005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23117-lldRO1-R2oDNA-lldRO2
creator: Anna Fomitcheva Khartchenko
created: 2015-08-25T11:00:00Z
modified: 2015-09-16T02:35:30Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _2273_
m user id: 26442
owner id: 26442
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Collection of promoters regulated by LldR.
mutable provenance: Synthetic synthesis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: R2oDNA spacer
sequence annotation: lldRO1
sequence annotation: J23117
sequence annotation: lldRO2
sequence: https://synbiohub.org/public/igem/BBa_K1847005_sequence/1

R2oDNA spacer
type: SequenceAnnotation
persistent identity: annotation2438061
display id: annotation2438061
version: 1
top level: BBa_K1847005
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1847005/annotation2438061/range2438061/1

https://synbiohub.org/public/igem/BBa_K1847005/annotation2438061/range2438061/1
type: Range
persistent identity: range2438061
display id: range2438061
version: 1
direction: direction/forward
top level: BBa_K1847005
start: 61
end: 169
orientation: inline

lldRO1
type: SequenceAnnotation
persistent identity: annotation2438060
display id: annotation2438060
version: 1
top level: BBa_K1847005
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K1847005/annotation2438060/range2438060/1

https://synbiohub.org/public/igem/BBa_K1847005/annotation2438060/range2438060/1
type: Range
persistent identity: range2438060
display id: range2438060
version: 1
direction: direction/forward
top level: BBa_K1847005
start: 44
end: 60
orientation: inline

J23117
type: SequenceAnnotation
persistent identity: annotation2438059
display id: annotation2438059
version: 1
top level: BBa_K1847005
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1847005/annotation2438059/range2438059/1

https://synbiohub.org/public/igem/BBa_K1847005/annotation2438059/range2438059/1
type: Range
persistent identity: range2438059
display id: range2438059
version: 1
direction: direction/forward
top level: BBa_K1847005
start: 1
end: 35
orientation: inline

lldRO2
type: SequenceAnnotation
persistent identity: annotation2438062
display id: annotation2438062
version: 1
top level: BBa_K1847005
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K1847005/annotation2438062/range2438062/1

https://synbiohub.org/public/igem/BBa_K1847005/annotation2438062/range2438062/1
type: Range
persistent identity: range2438062
display id: range2438062
version: 1
direction: direction/forward
top level: BBa_K1847005
start: 170
end: 186
orientation: inline

BBa_K1789001
type: ComponentDefinition
persistent identity: BBa K1789001
display id: BBa_K1789001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: IaaH
creator: Xinyuan Qiu
created: 2015-09-13T11:00:00Z
modified: 2015-09-14T05:49:28Z
discontinued: false
dominant: false
group u list: _2214_
m user id: 21134
owner id: 21134
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This part is the coding sequence of the enzyme Indoleacetimide hydrolase Iaa H. This enzyme catalyses the hydrolysis of indoleacetamide to indoleacetate and ammonia. This part is gathered from the 2-step pathway in Pseudomonas savastanoi producing the auxin, indole-3-acetic acid.
mutable notes: In order to get the whole sequence of IAAH, we removed the RBS and other irrelevant sequences in K515100 when designing the PCR primers.
mutable provenance: This part is gained from the part BBa_K515100 by PCR.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: IaaH
sequence: https://synbiohub.org/public/igem/BBa_K1789001_sequence/1

IaaH
type: SequenceAnnotation
persistent identity: annotation2454035
display id: annotation2454035
version: 1
top level: BBa_K1789001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1789001/annotation2454035/range2454035/1

https://synbiohub.org/public/igem/BBa_K1789001/annotation2454035/range2454035/1
type: Range
persistent identity: range2454035
display id: range2454035
version: 1
direction: direction/forward
top level: BBa_K1789001
start: 1
end: 1335
orientation: inline

BBa_K1640012
type: ComponentDefinition
persistent identity: BBa K1640012
display id: BBa_K1640012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: psbQR
creator: Meghan Cook
created: 2015-09-09T11:00:00Z
modified: 2015-09-10T01:02:41Z
discontinued: false
dominant: false
group u list: _2057_
m user id: 16353
owner id: 28565
owning group id: 9
status: status/Planning
bookmark: false
mutable description: n/a
mutable notes: n/a
mutable provenance: n/a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: rbs
sequence annotation: psbQ
sequence annotation: psbR
sequence annotation: double terminator
sequence: https://synbiohub.org/public/igem/BBa_K1640012_sequence/1

rbs
type: SequenceAnnotation
persistent identity: annotation2448233
display id: annotation2448233
version: 1
top level: BBa_K1640012
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1640012/annotation2448233/range2448233/1

https://synbiohub.org/public/igem/BBa_K1640012/annotation2448233/range2448233/1
type: Range
persistent identity: range2448233
display id: range2448233
version: 1
direction: direction/forward
top level: BBa_K1640012
start: 1
end: 19
orientation: inline

psbQ
type: SequenceAnnotation
persistent identity: annotation2448234
display id: annotation2448234
version: 1
top level: BBa_K1640012
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1640012/annotation2448234/range2448234/1

https://synbiohub.org/public/igem/BBa_K1640012/annotation2448234/range2448234/1
type: Range
persistent identity: range2448234
display id: range2448234
version: 1
direction: direction/forward
top level: BBa_K1640012
start: 20
end: 553
orientation: inline

psbR
type: SequenceAnnotation
persistent identity: annotation2448237
display id: annotation2448237
version: 1
top level: BBa_K1640012
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1640012/annotation2448237/range2448237/1

https://synbiohub.org/public/igem/BBa_K1640012/annotation2448237/range2448237/1
type: Range
persistent identity: range2448237
display id: range2448237
version: 1
direction: direction/forward
top level: BBa_K1640012
start: 571
end: 912
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2448236
display id: annotation2448236
version: 1
top level: BBa_K1640012
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1640012/annotation2448236/range2448236/1

https://synbiohub.org/public/igem/BBa_K1640012/annotation2448236/range2448236/1
type: Range
persistent identity: range2448236
display id: range2448236
version: 1
direction: direction/forward
top level: BBa_K1640012
start: 554
end: 570
orientation: inline

double terminator
type: SequenceAnnotation
persistent identity: annotation2448238
display id: annotation2448238
version: 1
top level: BBa_K1640012
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1640012/annotation2448238/range2448238/1

https://synbiohub.org/public/igem/BBa_K1640012/annotation2448238/range2448238/1
type: Range
persistent identity: range2448238
display id: range2448238
version: 1
direction: direction/forward
top level: BBa_K1640012
start: 913
end: 1041
orientation: inline

BBa_K1602050
type: ComponentDefinition
persistent identity: BBa K1602050
display id: BBa_K1602050
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RRlocked
creator: Christian Sator,Stefan Zens, Max Zander, Benedikt Spannenkrebs, Sebastian Jaeger
created: 2015-09-15T11:00:00Z
modified: 2015-09-25T05:13:21Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _2019_
m user id: 23028
owner id: 26220
owning group id: 9
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K1602050_sequence/1

GRE3
type: ComponentDefinition
persistent identity: BBa K1602004
display id: BBa_K1602004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Aldose reductase (GRE3) from Saccharomyces cerevisiae S288c
creator: Alexandra Goretzki, Tanja Habeck, Nina Kuschik-Maczollek, Laurin Monnheimer, Steven Pilger, Bianca Reisinger, Daniel Stumpf, Sebastian Barthel
created: 2015-08-31T11:00:00Z
modified: 2015-09-25T01:11:55Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2019_
m user id: 12512
owner id: 25989
owning group id: 9
status: status/Planning
bookmark: false
mutable description: BLANK
mutable notes: BLANK
mutable provenance: Saccharomyces cerevisiae
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1602004_sequence/1

BBa_K1632008
type: ComponentDefinition
persistent identity: BBa K1632008
display id: BBa_K1632008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: fim switch[default OFF](wild-type)_rbs_gfp
creator: Riku Shinohara
created: 2015-09-09T11:00:00Z
modified: 2015-09-15T07:04:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2049_
m user id: 24760
owner id: 22687
owning group id: 9
status: status/Planning
bookmark: false
mutable description: a
mutable notes: 1
mutable provenance: a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_I13500
component: BBa_K1632005
sequence annotation: BBa_I13500
sequence annotation: BBa_K1632005
sequence: https://synbiohub.org/public/igem/BBa_K1632008_sequence/1

BBa_I13500
type: Component
persistent identity: component2448485
display id: component2448485
version: 1
top level: BBa_K1632008
access: public
definition: https://synbiohub.org/public/igem/BBa_I13500/1

BBa_K1632005
type: Component
persistent identity: component2448481
display id: component2448481
version: 1
top level: BBa_K1632008
access: public
definition: BBa_K1632005

BBa_I13500
type: SequenceAnnotation
persistent identity: annotation2448485
display id: annotation2448485
version: 1
top level: BBa_K1632008
location: https://synbiohub.org/public/igem/BBa_K1632008/annotation2448485/range2448485/1
component: BBa_I13500

https://synbiohub.org/public/igem/BBa_K1632008/annotation2448485/range2448485/1
type: Range
persistent identity: range2448485
display id: range2448485
version: 1
direction: direction/reverse
top level: BBa_K1632008
start: 391
end: 1128
orientation: inline

BBa_K1632005
type: SequenceAnnotation
persistent identity: annotation2448481
display id: annotation2448481
version: 1
top level: BBa_K1632008
location: https://synbiohub.org/public/igem/BBa_K1632008/annotation2448481/range2448481/1
component: BBa_K1632005

https://synbiohub.org/public/igem/BBa_K1632008/annotation2448481/range2448481/1
type: Range
persistent identity: range2448481
display id: range2448481
version: 1
direction: direction/reverse
top level: BBa_K1632008
start: 1
end: 382
orientation: inline

BBa_K1850013
type: ComponentDefinition
persistent identity: BBa K1850013
display id: BBa_K1850013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pAra - fim
creator: Lydia Goldberg
created: 2015-09-14T11:00:00Z
modified: 2015-09-16T11:00:54Z
discontinued: false
dominant: false
group u list: _2277_
m user id: 26421
owner id: 26421
owning group id: 9
status: status/Planning
bookmark: false
mutable description: placeholder
mutable notes: placeholder
mutable provenance: placeholder
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: pAra
sequence annotation: fimG
sequence annotation: fimC
sequence annotation: fimF
sequence annotation: fimI
sequence annotation: fimA
sequence annotation: fimD
sequence: https://synbiohub.org/public/igem/BBa_K1850013_sequence/1

pAra
type: SequenceAnnotation
persistent identity: annotation2463810
display id: annotation2463810
version: 1
top level: BBa_K1850013
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1850013/annotation2463810/range2463810/1

https://synbiohub.org/public/igem/BBa_K1850013/annotation2463810/range2463810/1
type: Range
persistent identity: range2463810
display id: range2463810
version: 1
direction: direction/forward
top level: BBa_K1850013
start: 1
end: 130
orientation: inline

fimG
type: SequenceAnnotation
persistent identity: annotation2463816
display id: annotation2463816
version: 1
top level: BBa_K1850013
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1850013/annotation2463816/range2463816/1

https://synbiohub.org/public/igem/BBa_K1850013/annotation2463816/range2463816/1
type: Range
persistent identity: range2463816
display id: range2463816
version: 1
direction: direction/forward
top level: BBa_K1850013
start: 5371
end: 5874
orientation: inline

fimC
type: SequenceAnnotation
persistent identity: annotation2463812
display id: annotation2463812
version: 1
top level: BBa_K1850013
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1850013/annotation2463812/range2463812/1

https://synbiohub.org/public/igem/BBa_K1850013/annotation2463812/range2463812/1
type: Range
persistent identity: range2463812
display id: range2463812
version: 1
direction: direction/forward
top level: BBa_K1850013
start: 1390
end: 2115
orientation: inline

fimF
type: SequenceAnnotation
persistent identity: annotation2463815
display id: annotation2463815
version: 1
top level: BBa_K1850013
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1850013/annotation2463815/range2463815/1

https://synbiohub.org/public/igem/BBa_K1850013/annotation2463815/range2463815/1
type: Range
persistent identity: range2463815
display id: range2463815
version: 1
direction: direction/forward
top level: BBa_K1850013
start: 4828
end: 5358
orientation: inline

fimI
type: SequenceAnnotation
persistent identity: annotation2463811
display id: annotation2463811
version: 1
top level: BBa_K1850013
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1850013/annotation2463811/range2463811/1

https://synbiohub.org/public/igem/BBa_K1850013/annotation2463811/range2463811/1
type: Range
persistent identity: range2463811
display id: range2463811
version: 1
direction: direction/forward
top level: BBa_K1850013
start: 814
end: 1353
orientation: inline

fimA
type: SequenceAnnotation
persistent identity: annotation2463809
display id: annotation2463809
version: 1
top level: BBa_K1850013
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1850013/annotation2463809/range2463809/1

https://synbiohub.org/public/igem/BBa_K1850013/annotation2463809/range2463809/1
type: Range
persistent identity: range2463809
display id: range2463809
version: 1
direction: direction/forward
top level: BBa_K1850013
start: 201
end: 749
orientation: inline

fimD
type: SequenceAnnotation
persistent identity: annotation2463813
display id: annotation2463813
version: 1
top level: BBa_K1850013
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1850013/annotation2463813/range2463813/1

https://synbiohub.org/public/igem/BBa_K1850013/annotation2463813/range2463813/1
type: Range
persistent identity: range2463813
display id: range2463813
version: 1
direction: direction/forward
top level: BBa_K1850013
start: 2182
end: 4818
orientation: inline

BBa_K1847003
type: ComponentDefinition
persistent identity: BBa K1847003
display id: BBa_K1847003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23117-lldRO1-lldRO2
creator: Anna Fomitcheva Khartchenko
created: 2015-08-25T11:00:00Z
modified: 2015-08-26T04:21:58Z
discontinued: false
dominant: false
group u list: _2273_
m user id: 26442
owner id: 26442
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Collection of promoters regulated by LldR
mutable provenance: Synthetic syntehsis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: J23117
sequence annotation: lldRO2
sequence annotation: lldRO1
sequence: https://synbiohub.org/public/igem/BBa_K1847003_sequence/1

J23117
type: SequenceAnnotation
persistent identity: annotation2438051
display id: annotation2438051
version: 1
top level: BBa_K1847003
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1847003/annotation2438051/range2438051/1

https://synbiohub.org/public/igem/BBa_K1847003/annotation2438051/range2438051/1
type: Range
persistent identity: range2438051
display id: range2438051
version: 1
direction: direction/forward
top level: BBa_K1847003
start: 1
end: 35
orientation: inline

lldRO2
type: SequenceAnnotation
persistent identity: annotation2438053
display id: annotation2438053
version: 1
top level: BBa_K1847003
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K1847003/annotation2438053/range2438053/1

https://synbiohub.org/public/igem/BBa_K1847003/annotation2438053/range2438053/1
type: Range
persistent identity: range2438053
display id: range2438053
version: 1
direction: direction/forward
top level: BBa_K1847003
start: 61
end: 77
orientation: inline

lldRO1
type: SequenceAnnotation
persistent identity: annotation2438052
display id: annotation2438052
version: 1
top level: BBa_K1847003
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K1847003/annotation2438052/range2438052/1

https://synbiohub.org/public/igem/BBa_K1847003/annotation2438052/range2438052/1
type: Range
persistent identity: range2438052
display id: range2438052
version: 1
direction: direction/forward
top level: BBa_K1847003
start: 44
end: 60
orientation: inline

His-Scaffo
type: ComponentDefinition
persistent identity: BBa K1602027
display id: BBa_K1602027
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Protein scaffold with N-terminal HisTag
creator: Sebastian Barthel, Niklas Hummel, Jasmin Weber, Michelle Zoeller, Marcus Geissler, Stefan Bohn, Simon Linder
created: 2015-09-15T11:00:00Z
modified: 2015-09-16T06:19:29Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2019_
m user id: 22022
owner id: 22022
owning group id: 9
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1602027_sequence/1

BBa_K1692003
type: ComponentDefinition
persistent identity: BBa K1692003
display id: BBa_K1692003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PAL with T7 promoter
creator: Daniel Kunin
created: 2015-08-02T11:00:00Z
modified: 2015-08-03T01:03:09Z
discontinued: false
dominant: false
group u list: _2110_
m user id: 26209
owner id: 26209
owning group id: 9
status: status/Planning
bookmark: false
mutable description: PAL with T7 promoter
mutable notes: None
mutable provenance: University British Columbia 2013 iGEM
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1129003
component: BBa_K525998
sequence annotation: BBa_K525998
sequence annotation: BBa_K1129003
sequence: https://synbiohub.org/public/igem/BBa_K1692003_sequence/1

BBa_K1129003
type: Component
persistent identity: component2433987
display id: component2433987
version: 1
top level: BBa_K1692003
access: public
definition: https://synbiohub.org/public/igem/BBa_K1129003/1

BBa_K525998
type: Component
persistent identity: component2433986
display id: component2433986
version: 1
top level: BBa_K1692003
access: public
definition: https://synbiohub.org/public/igem/BBa_K525998/1

BBa_K525998
type: SequenceAnnotation
persistent identity: annotation2433986
display id: annotation2433986
version: 1
top level: BBa_K1692003
location: https://synbiohub.org/public/igem/BBa_K1692003/annotation2433986/range2433986/1
component: BBa_K525998

https://synbiohub.org/public/igem/BBa_K1692003/annotation2433986/range2433986/1
type: Range
persistent identity: range2433986
display id: range2433986
version: 1
direction: direction/reverse
top level: BBa_K1692003
start: 1
end: 32
orientation: inline

BBa_K1129003
type: SequenceAnnotation
persistent identity: annotation2433987
display id: annotation2433987
version: 1
top level: BBa_K1692003
location: https://synbiohub.org/public/igem/BBa_K1692003/annotation2433987/range2433987/1
component: BBa_K1129003

https://synbiohub.org/public/igem/BBa_K1692003/annotation2433987/range2433987/1
type: Range
persistent identity: range2433987
display id: range2433987
version: 1
direction: direction/reverse
top level: BBa_K1692003
start: 39
end: 1610
orientation: inline

BBa_K1763004
type: ComponentDefinition
persistent identity: BBa K1763004
display id: BBa_K1763004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Major Spidroin Protein 2 (MaSp2) with sticky ends CA
creator: Vinson Lam
created: 2015-06-01T11:00:00Z
modified: 2015-09-18T10:53:36Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2185_
m user id: 27136
owner id: 27136
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This part contains the core sequence of MaSp2 that has been assembled with a 5'-CGTG-3' overhang on the 5' end of the sequence and 3'-TCAA-5' overhang on the 3' end of the sequence. This part was designed for use with ICA, along with parts BBa_K1763003 and BBa_K1763004 as an efficient method to assemble multiple MaSp2 sequences together. This setup is to show that this particular set of sticky ends is suitable for ICA. Before using in ICA, this part should be digested with BsaI, a type IIs restriction enzyme, which cuts DNA outside of its recognition site.
mutable notes: The gene sequence for MaSp2 is particularly GC-rich, which may complicate PCR.
mutable provenance: This part is derived from the MaSp2 gene found in the spider N. Clavipes.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: BsaI recognition site (reverse)
sequence annotation: A sticky end
sequence annotation: BsaI recognition site (forward)
sequence annotation: MaSp2 Core
sequence annotation: C sticky end
sequence: https://synbiohub.org/public/igem/BBa_K1763004_sequence/1

BsaI recognition site (reverse)
type: SequenceAnnotation
persistent identity: annotation2432570
display id: annotation2432570
version: 1
top level: BBa_K1763004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1763004/annotation2432570/range2432570/1

https://synbiohub.org/public/igem/BBa_K1763004/annotation2432570/range2432570/1
type: Range
persistent identity: range2432570
display id: range2432570
version: 1
direction: direction/reverse
top level: BBa_K1763004
start: 114
end: 119
orientation: inline

A sticky end
type: SequenceAnnotation
persistent identity: annotation2463765
display id: annotation2463765
version: 1
top level: BBa_K1763004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1763004/annotation2463765/range2463765/1

https://synbiohub.org/public/igem/BBa_K1763004/annotation2463765/range2463765/1
type: Range
persistent identity: range2463765
display id: range2463765
version: 1
direction: direction/forward
top level: BBa_K1763004
start: 109
end: 112
orientation: inline

BsaI recognition site (forward)
type: SequenceAnnotation
persistent identity: annotation2432569
display id: annotation2432569
version: 1
top level: BBa_K1763004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1763004/annotation2432569/range2432569/1

https://synbiohub.org/public/igem/BBa_K1763004/annotation2432569/range2432569/1
type: Range
persistent identity: range2432569
display id: range2432569
version: 1
direction: direction/forward
top level: BBa_K1763004
start: 1
end: 5
orientation: inline

MaSp2 Core
type: SequenceAnnotation
persistent identity: annotation2473129
display id: annotation2473129
version: 1
top level: BBa_K1763004
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763004/annotation2473129/range2473129/1

https://synbiohub.org/public/igem/BBa_K1763004/annotation2473129/range2473129/1
type: Range
persistent identity: range2473129
display id: range2473129
version: 1
direction: direction/forward
top level: BBa_K1763004
start: 10
end: 108
orientation: inline

C sticky end
type: SequenceAnnotation
persistent identity: annotation2463764
display id: annotation2463764
version: 1
top level: BBa_K1763004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1763004/annotation2463764/range2463764/1

https://synbiohub.org/public/igem/BBa_K1763004/annotation2463764/range2463764/1
type: Range
persistent identity: range2463764
display id: range2463764
version: 1
direction: direction/forward
top level: BBa_K1763004
start: 7
end: 10
orientation: inline

DsbDspB
type: ComponentDefinition
persistent identity: BBa K1659201
display id: BBa_K1659201
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Dispersin B fused at N-terminal with DsbA 2-19 signal sequence
creator: Wei Chung Kong
created: 2015-08-27T11:00:00Z
modified: 2015-09-14T03:05:35Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2077_
m user id: 24524
owner id: 24524
owning group id: 9
status: status/Planning
bookmark: false
mutable description: to be updated
mutable notes: to be updated
mutable provenance: to be updated
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: DspB
sequence annotation: Hisx6
sequence annotation: stop
sequence annotation: DsbA 2-19 Signal Sequence
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_K1659201_sequence/1

DspB
type: SequenceAnnotation
persistent identity: annotation2449115
display id: annotation2449115
version: 1
top level: DsbDspB
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1659201/annotation2449115/range2449115/1

https://synbiohub.org/public/igem/BBa_K1659201/annotation2449115/range2449115/1
type: Range
persistent identity: range2449115
display id: range2449115
version: 1
direction: direction/forward
top level: DsbDspB
start: 58
end: 1140
orientation: inline

Hisx6
type: SequenceAnnotation
persistent identity: annotation2449116
display id: annotation2449116
version: 1
top level: DsbDspB
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1659201/annotation2449116/range2449116/1

https://synbiohub.org/public/igem/BBa_K1659201/annotation2449116/range2449116/1
type: Range
persistent identity: range2449116
display id: range2449116
version: 1
direction: direction/forward
top level: DsbDspB
start: 1141
end: 1158
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2453897
display id: annotation2453897
version: 1
top level: DsbDspB
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1659201/annotation2453897/range2453897/1

https://synbiohub.org/public/igem/BBa_K1659201/annotation2453897/range2453897/1
type: Range
persistent identity: range2453897
display id: range2453897
version: 1
direction: direction/forward
top level: DsbDspB
start: 1159
end: 1164
orientation: inline

DsbA 2-19 Signal Sequence
type: SequenceAnnotation
persistent identity: annotation2449114
display id: annotation2449114
version: 1
top level: DsbDspB
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1659201/annotation2449114/range2449114/1

https://synbiohub.org/public/igem/BBa_K1659201/annotation2449114/range2449114/1
type: Range
persistent identity: range2449114
display id: range2449114
version: 1
direction: direction/forward
top level: DsbDspB
start: 4
end: 57
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2449113
display id: annotation2449113
version: 1
top level: DsbDspB
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1659201/annotation2449113/range2449113/1

https://synbiohub.org/public/igem/BBa_K1659201/annotation2449113/range2449113/1
type: Range
persistent identity: range2449113
display id: range2449113
version: 1
direction: direction/forward
top level: DsbDspB
start: 1
end: 3
orientation: inline

BBa_K1789004
type: ComponentDefinition
persistent identity: BBa K1789004
display id: BBa_K1789004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP2
creator: Xinyuan Qiu
created: 2015-09-13T11:00:00Z
modified: 2016-01-25T01:04:48Z
discontinued: false
dominant: false
group u list: _2214_
m user id: 4206
owner id: 21134
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This part is the Carboxyl Half of GFP
mutable notes: we REMOVED the extra base from part BBa_I715020(easier to fuse with other proteins) when designing PCR primers to make the protein fusion easier.
mutable provenance: This part is gained from part BBa_I715020.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: GFP2
sequence: https://synbiohub.org/public/igem/BBa_K1789004_sequence/1

GFP2
type: SequenceAnnotation
persistent identity: annotation2454067
display id: annotation2454067
version: 1
top level: BBa_K1789004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1789004/annotation2454067/range2454067/1

https://synbiohub.org/public/igem/BBa_K1789004/annotation2454067/range2454067/1
type: Range
persistent identity: range2454067
display id: range2454067
version: 1
direction: direction/forward
top level: BBa_K1789004
start: 1
end: 249
orientation: inline

BBa_K1795018
type: ComponentDefinition
persistent identity: BBa K1795018
display id: BBa_K1795018
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CFP-LVA under promoter I13453
creator: Andrew D Halleran, Michael C LeFew, Joseph L Maniaci, John P Marken, Caroline A Golino
created: 2015-09-16T11:00:00Z
modified: 2015-09-17T09:25:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2220_
m user id: 27446
owner id: 27446
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Cyan fluorescent protein with LVA fast degradation tag under the control of pBad promoter I13453.
mutable provenance: iGEM registry parts
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0015
component: BBa_E0022
component: BBa_I13453
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_I13453
sequence annotation: BBa_E0022
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K1795018_sequence/1

BBa_B0015
type: Component
persistent identity: component2468253
display id: component2468253
version: 1
top level: BBa_K1795018
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_E0022
type: Component
persistent identity: component2468243
display id: component2468243
version: 1
top level: BBa_K1795018
access: public
definition: https://synbiohub.org/public/igem/BBa_E0022/1

BBa_I13453
type: Component
persistent identity: component2468239
display id: component2468239
version: 1
top level: BBa_K1795018
access: public
definition: https://synbiohub.org/public/igem/BBa_I13453/1

BBa_B0034
type: Component
persistent identity: component2468241
display id: component2468241
version: 1
top level: BBa_K1795018
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2468241
display id: annotation2468241
version: 1
top level: BBa_K1795018
location: https://synbiohub.org/public/igem/BBa_K1795018/annotation2468241/range2468241/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1795018/annotation2468241/range2468241/1
type: Range
persistent identity: range2468241
display id: range2468241
version: 1
direction: direction/reverse
top level: BBa_K1795018
start: 139
end: 150
orientation: inline

BBa_I13453
type: SequenceAnnotation
persistent identity: annotation2468239
display id: annotation2468239
version: 1
top level: BBa_K1795018
location: https://synbiohub.org/public/igem/BBa_K1795018/annotation2468239/range2468239/1
component: BBa_I13453

https://synbiohub.org/public/igem/BBa_K1795018/annotation2468239/range2468239/1
type: Range
persistent identity: range2468239
display id: range2468239
version: 1
direction: direction/reverse
top level: BBa_K1795018
start: 1
end: 130
orientation: inline

BBa_E0022
type: SequenceAnnotation
persistent identity: annotation2468243
display id: annotation2468243
version: 1
top level: BBa_K1795018
location: https://synbiohub.org/public/igem/BBa_K1795018/annotation2468243/range2468243/1
component: BBa_E0022

https://synbiohub.org/public/igem/BBa_K1795018/annotation2468243/range2468243/1
type: Range
persistent identity: range2468243
display id: range2468243
version: 1
direction: direction/forward
top level: BBa_K1795018
start: 157
end: 918
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2468253
display id: annotation2468253
version: 1
top level: BBa_K1795018
location: https://synbiohub.org/public/igem/BBa_K1795018/annotation2468253/range2468253/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1795018/annotation2468253/range2468253/1
type: Range
persistent identity: range2468253
display id: range2468253
version: 1
direction: direction/reverse
top level: BBa_K1795018
start: 927
end: 1055
orientation: inline

BBa_K1602051
type: ComponentDefinition
persistent identity: BBa K1602051
display id: BBa_K1602051
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: araCpBad-RRkey
creator: Christian Sator,Stefan Zens, Max Zander, Benedikt Spannenkrebs, Sebastian Jaeger
created: 2015-09-15T11:00:00Z
modified: 2015-09-25T05:11:55Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _2019_
m user id: 23028
owner id: 26220
owning group id: 9
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1602049
component: BBa_K808000
sequence annotation: BBa_K808000
sequence annotation: BBa_K1602049
sequence: https://synbiohub.org/public/igem/BBa_K1602051_sequence/1

BBa_K1602049
type: Component
persistent identity: component2462790
display id: component2462790
version: 1
top level: BBa_K1602051
access: public
definition: BBa_K1602049

BBa_K808000
type: Component
persistent identity: component2462789
display id: component2462789
version: 1
top level: BBa_K1602051
access: public
definition: https://synbiohub.org/public/igem/BBa_K808000/1

BBa_K808000
type: SequenceAnnotation
persistent identity: annotation2462789
display id: annotation2462789
version: 1
top level: BBa_K1602051
location: https://synbiohub.org/public/igem/BBa_K1602051/annotation2462789/range2462789/1
component: BBa_K808000

https://synbiohub.org/public/igem/BBa_K1602051/annotation2462789/range2462789/1
type: Range
persistent identity: range2462789
display id: range2462789
version: 1
direction: direction/reverse
top level: BBa_K1602051
start: 1
end: 1209
orientation: inline

BBa_K1602049
type: SequenceAnnotation
persistent identity: annotation2462790
display id: annotation2462790
version: 1
top level: BBa_K1602051
location: https://synbiohub.org/public/igem/BBa_K1602051/annotation2462790/range2462790/1
component: BBa_K1602049

https://synbiohub.org/public/igem/BBa_K1602051/annotation2462790/range2462790/1
type: Range
persistent identity: range2462790
display id: range2462790
version: 1
direction: direction/reverse
top level: BBa_K1602051
start: 1218
end: 1288
orientation: inline

BBa_K1674003
type: ComponentDefinition
persistent identity: BBa K1674003
display id: BBa_K1674003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: tsPurple Reporter + 3-alpha-HSD (RBS + Coding + Terminator)
creator: Adi Yannai
created: 2015-09-14T11:00:00Z
modified: 2015-09-18T02:25:56Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2092_
m user id: 25765
owner id: 25765
owning group id: 9
status: status/Planning
bookmark: false
mutable description: tsPurple Reporter + 3α- HSD
mutable notes: tsPurple Reporter + 3α- HSD
mutable provenance: tsPurple Reporter + 3α- HSD
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1357008
component: BBa_K1321338
component: BBa_K1674001
sequence annotation: BBa_K1321338
sequence annotation: BBa_K1357008
sequence annotation: BBa_K1674001
sequence: https://synbiohub.org/public/igem/BBa_K1674003_sequence/1

BBa_K1357008
type: Component
persistent identity: component2457671
display id: component2457671
version: 1
top level: BBa_K1674003
access: public
definition: https://synbiohub.org/public/igem/BBa_K1357008/1

BBa_K1321338
type: Component
persistent identity: component2457658
display id: component2457658
version: 1
top level: BBa_K1674003
access: public
definition: https://synbiohub.org/public/igem/BBa_K1321338/1

BBa_K1674001
type: Component
persistent identity: component2457659
display id: component2457659
version: 1
top level: BBa_K1674003
access: public
definition: 3alpha-HSD

BBa_K1321338
type: SequenceAnnotation
persistent identity: annotation2457658
display id: annotation2457658
version: 1
top level: BBa_K1674003
location: https://synbiohub.org/public/igem/BBa_K1674003/annotation2457658/range2457658/1
component: BBa_K1321338

https://synbiohub.org/public/igem/BBa_K1674003/annotation2457658/range2457658/1
type: Range
persistent identity: range2457658
display id: range2457658
version: 1
direction: direction/reverse
top level: BBa_K1674003
start: 1
end: 43
orientation: inline

BBa_K1357008
type: SequenceAnnotation
persistent identity: annotation2457671
display id: annotation2457671
version: 1
top level: BBa_K1674003
location: https://synbiohub.org/public/igem/BBa_K1674003/annotation2457671/range2457671/1
component: BBa_K1357008

https://synbiohub.org/public/igem/BBa_K1674003/annotation2457671/range2457671/1
type: Range
persistent identity: range2457671
display id: range2457671
version: 1
direction: direction/reverse
top level: BBa_K1674003
start: 1027
end: 1871
orientation: inline

BBa_K1674001
type: SequenceAnnotation
persistent identity: annotation2457659
display id: annotation2457659
version: 1
top level: BBa_K1674003
location: https://synbiohub.org/public/igem/BBa_K1674003/annotation2457659/range2457659/1
component: BBa_K1674001

https://synbiohub.org/public/igem/BBa_K1674003/annotation2457659/range2457659/1
type: Range
persistent identity: range2457659
display id: range2457659
version: 1
direction: direction/reverse
top level: BBa_K1674003
start: 50
end: 1018
orientation: inline

Tamura
type: ComponentDefinition
persistent identity: BBa K1763444
display id: BBa_K1763444
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Bombyx mori silk co-spinning module
creator: Fasih Ahsan
created: 2015-09-14T11:00:00Z
modified: 2016-02-10T11:26:30Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2185_
m user id: 4206
owner id: 22156
owning group id: 9
status: status/Planning
bookmark: true
mutable description: Studies investigating the potential for co-spinning, a process in which the terminal domains of the functional B. mori silk protein heavy chain are fused with genes encoding functional binding domains, and subsequently are combined with native silk proteins to form crystalline fibers, have shown some success (Sponner et al., 2005). In vivo co-spinning simulations have previously been conducted using the transgene in silkworms, producing functionally fluorescent N-terminal ??? GFP- C-terminal fusions that successfully incorporate into endogenous Bombyx mori silkworm silk monomers (Kojima et. al., 2007). These N-Terminal and C-terminal regions are not only easier to clone due to their non-repetitive structures, but additionally allows for storage and assembly of endogenous ampullate spidroins (Askarieh et al., 2010). Composite silkworm-spider silk chimeras have shown improved mechanical properties, and may increase the ease in high-yield production due to the easier manipulation of silkworm silk monomers (Teule, et. al. 2012). Yet, this process still relies on the use of transgenic silkworms, which have longer developmental cycles, require larger storage and nutrient conditions, and difficulty to harvest relative to bacterial counterparts (Murphy et al., 2009). An ex situ co-spinning process has been developed for the production of composite N. clavipes ??? B. mori silk fibers with functional protein fusions in bacterial chassis (namely, E. coli). This process results in the construction of a transgene that can be modularized using restriction enzymes to incorporate a wide range of functional peptide binding sites or other proteins, including avidin-biotin binding domains, RGD-cadherin motifs, albumin binding domains, or antibody affinity domains (Jansson et al., 2014). Specifically, this composite structure focuses on the ability to express functional sfGFP in silk fibers as a marker for successful co-spinning, and verifies proper NC-sfGFP expression using SDS-PAGE a…
mutable notes: We decided to use a LacI promoter to regulate expression of the large protein construct in E. coli K12 laboratory strains, in lieu of utilizing a T7 promoter or constitutive Anderson family promoters. This is to maximize the protein yield of the co-spinning module without causing a tradeoff between regular cell growth and housekeeping protein synthesis. Additionally, we decided to use sfGFP as the reporter gene, instead of a standard mut3b GFP, due to the ability of sfGFP to function even when fused to large bulky protein domains (in this case, FibNT and FibCT). Lastly, we designed BsaI TypeIIS restriction sites flanking the reporter gene that when cut, generates sticky ends compatible with EcoRI and SpeI sites. This was created to allow iGEM teams to take protein domains of interest from the Standard Registry of Parts, and fuse them into the co-spinning module by a simple Digest and Ligate assembly to swap the sfGFP construct. However, the protein domain to be added must have the Silver Fusion prefix and suffix, to allow for in-frame cloning of the protein domain. Without this in-frame cloning, the scar sites of the tradition prefix and suffix will cause a frameshift mutation that mutate the translation product, and renders it non-functional.
mutable provenance: The LacI promoter (BBa_R0010), RBS (BBa_B0034), and super folder GFP (BBa_I746916) were derived from sequences found in the Standard Registry of Parts. The Bombyx mori silk fibroin heavy chain N-terminal (FibNT) and C-terminal (FibCT) domains were derived from the sequenced consensus genome of Bombyx mori silkworm species, and were synthesized using IDT gBlocks gene fragments.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: B. mori FibNT
sequence annotation: 6x N-terminal polyhistidine tag
sequence annotation: Elowitz RBS (BBa_B0034)
sequence annotation: LacI (BBa_R0010)
sequence annotation: B. mori FibCT
sequence annotation: Bba prefix/suffix assembly scar site
sequence annotation: superfolder GFP (BBa_I746916)
sequence annotation: Ribosome Double-Stop Site
sequence annotation: BsaI Restriction Site (EcoRI Compatible)
sequence: https://synbiohub.org/public/igem/BBa_K1763444_sequence/1

B. mori FibNT
type: SequenceAnnotation
persistent identity: annotation2462436
display id: annotation2462436
version: 1
top level: Tamura
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1763444/annotation2462436/range2462436/1

https://synbiohub.org/public/igem/BBa_K1763444/annotation2462436/range2462436/1
type: Range
persistent identity: range2462436
display id: range2462436
version: 1
direction: direction/forward
top level: Tamura
start: 248
end: 712
orientation: inline

6x N-terminal polyhistidine tag
type: SequenceAnnotation
persistent identity: annotation2462435
display id: annotation2462435
version: 1
top level: Tamura
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1763444/annotation2462435/range2462435/1

https://synbiohub.org/public/igem/BBa_K1763444/annotation2462435/range2462435/1
type: Range
persistent identity: range2462435
display id: range2462435
version: 1
direction: direction/forward
top level: Tamura
start: 230
end: 247
orientation: inline

Elowitz RBS (BBa_B0034)
type: SequenceAnnotation
persistent identity: annotation2462434
display id: annotation2462434
version: 1
top level: Tamura
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1763444/annotation2462434/range2462434/1

https://synbiohub.org/public/igem/BBa_K1763444/annotation2462434/range2462434/1
type: Range
persistent identity: range2462434
display id: range2462434
version: 1
direction: direction/forward
top level: Tamura
start: 209
end: 220
orientation: inline

LacI (BBa_R0010)
type: SequenceAnnotation
persistent identity: annotation2462432
display id: annotation2462432
version: 1
top level: Tamura
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1763444/annotation2462432/range2462432/1

https://synbiohub.org/public/igem/BBa_K1763444/annotation2462432/range2462432/1
type: Range
persistent identity: range2462432
display id: range2462432
version: 1
direction: direction/forward
top level: Tamura
start: 1
end: 200
orientation: inline

B. mori FibCT
type: SequenceAnnotation
persistent identity: annotation2462439
display id: annotation2462439
version: 1
top level: Tamura
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1763444/annotation2462439/range2462439/1

https://synbiohub.org/public/igem/BBa_K1763444/annotation2462439/range2462439/1
type: Range
persistent identity: range2462439
display id: range2462439
version: 1
direction: direction/forward
top level: Tamura
start: 1451
end: 1627
orientation: inline

Bba prefix/suffix assembly scar site
type: SequenceAnnotation
persistent identity: annotation2462433
display id: annotation2462433
version: 1
top level: Tamura
role: feature/scar
location: https://synbiohub.org/public/igem/BBa_K1763444/annotation2462433/range2462433/1

https://synbiohub.org/public/igem/BBa_K1763444/annotation2462433/range2462433/1
type: Range
persistent identity: range2462433
display id: range2462433
version: 1
direction: direction/forward
top level: Tamura
start: 201
end: 208
orientation: inline

superfolder GFP (BBa_I746916)
type: SequenceAnnotation
persistent identity: annotation2462438
display id: annotation2462438
version: 1
top level: Tamura
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1763444/annotation2462438/range2462438/1

https://synbiohub.org/public/igem/BBa_K1763444/annotation2462438/range2462438/1
type: Range
persistent identity: range2462438
display id: range2462438
version: 1
direction: direction/forward
top level: Tamura
start: 725
end: 1438
orientation: inline

Ribosome Double-Stop Site
type: SequenceAnnotation
persistent identity: annotation2462440
display id: annotation2462440
version: 1
top level: Tamura
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1763444/annotation2462440/range2462440/1

https://synbiohub.org/public/igem/BBa_K1763444/annotation2462440/range2462440/1
type: Range
persistent identity: range2462440
display id: range2462440
version: 1
direction: direction/forward
top level: Tamura
start: 1628
end: 1633
orientation: inline

BsaI Restriction Site (EcoRI Compatible)
type: SequenceAnnotation
persistent identity: annotation2462437
display id: annotation2462437
version: 1
top level: Tamura
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763444/annotation2462437/range2462437/1

https://synbiohub.org/public/igem/BBa_K1763444/annotation2462437/range2462437/1
type: Range
persistent identity: range2462437
display id: range2462437
version: 1
direction: direction/forward
top level: Tamura
start: 719
end: 724
orientation: inline

BBa_K1585310
type: ComponentDefinition
persistent identity: BBa K1585310
display id: BBa_K1585310
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0034.GlgA
creator: Laura Helleckes, Michael Osthege, Sarah Lubjuhn
created: 2015-08-24T11:00:00Z
modified: 2015-09-17T02:20:38Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2002_
m user id: 24474
owner id: 24505
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This is the translational unit of GlgA (Glycogen synthase) for elongation of glycogen chains. Through autophosphorylation, GlgA is also the starting point of glycogen synthesis in bacteria.
mutable notes: -
mutable provenance: E. coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0034
component: BBa_K1585300
sequence annotation: BBa_B0034
sequence annotation: BBa_K1585300
sequence: https://synbiohub.org/public/igem/BBa_K1585310_sequence/1

BBa_B0034
type: Component
persistent identity: component2437901
display id: component2437901
version: 1
top level: BBa_K1585310
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1585300
type: Component
persistent identity: component2437909
display id: component2437909
version: 1
top level: BBa_K1585310
access: public
definition: https://synbiohub.org/public/igem/BBa_K1585300/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2437901
display id: annotation2437901
version: 1
top level: BBa_K1585310
location: https://synbiohub.org/public/igem/BBa_K1585310/annotation2437901/range2437901/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1585310/annotation2437901/range2437901/1
type: Range
persistent identity: range2437901
display id: range2437901
version: 1
direction: direction/reverse
top level: BBa_K1585310
start: 1
end: 12
orientation: inline

BBa_K1585300
type: SequenceAnnotation
persistent identity: annotation2437909
display id: annotation2437909
version: 1
top level: BBa_K1585310
location: https://synbiohub.org/public/igem/BBa_K1585310/annotation2437909/range2437909/1
component: BBa_K1585300

https://synbiohub.org/public/igem/BBa_K1585310/annotation2437909/range2437909/1
type: Range
persistent identity: range2437909
display id: range2437909
version: 1
direction: direction/reverse
top level: BBa_K1585310
start: 19
end: 1455
orientation: inline

BBa_K1639004
type: ComponentDefinition
persistent identity: BBa K1639004
display id: BBa_K1639004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: H-NS
creator: Mustafa Yılmaz
created: 2015-09-11T11:00:00Z
modified: 2015-09-18T07:25:52Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2056_
m user id: 20835
owner id: 20835
owning group id: 9
status: status/Planning
bookmark: false
mutable description: global DNA-binding transcriptional dual regulator H-NS
mutable notes: This part contains an additional BamHI restriction site at beginning of TlpB sequence and a XhoI restriction site at the end.
mutable provenance: Escherichia Coli K-12, sequence taken from Genbank: http://www.ncbi.nlm.nih.gov/gene/945829
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Stop Codon
sequence annotation: ATG Start Codon
sequence annotation: H-NS
sequence: https://synbiohub.org/public/igem/BBa_K1639004_sequence/1

Stop Codon
type: SequenceAnnotation
persistent identity: annotation2452958
display id: annotation2452958
version: 1
top level: BBa_K1639004
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1639004/annotation2452958/range2452958/1

https://synbiohub.org/public/igem/BBa_K1639004/annotation2452958/range2452958/1
type: Range
persistent identity: range2452958
display id: range2452958
version: 1
direction: direction/forward
top level: BBa_K1639004
start: 420
end: 426
orientation: inline

ATG Start Codon
type: SequenceAnnotation
persistent identity: annotation2452956
display id: annotation2452956
version: 1
top level: BBa_K1639004
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1639004/annotation2452956/range2452956/1

https://synbiohub.org/public/igem/BBa_K1639004/annotation2452956/range2452956/1
type: Range
persistent identity: range2452956
display id: range2452956
version: 1
direction: direction/forward
top level: BBa_K1639004
start: 9
end: 11
orientation: inline

H-NS
type: SequenceAnnotation
persistent identity: annotation2452955
display id: annotation2452955
version: 1
top level: BBa_K1639004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1639004/annotation2452955/range2452955/1

https://synbiohub.org/public/igem/BBa_K1639004/annotation2452955/range2452955/1
type: Range
persistent identity: range2452955
display id: range2452955
version: 1
direction: direction/forward
top level: BBa_K1639004
start: 9
end: 422
orientation: inline

BBa_K1800002
type: ComponentDefinition
persistent identity: BBa K1800002
display id: BBa_K1800002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Horseradish Peroxidase
creator: Laura Lee Irvin
created: 2015-08-27T11:00:00Z
modified: 2015-12-16T01:32:07Z
discontinued: false
dominant: false
group u list: _2225_
m user id: 4206
owner id: 27447
owning group id: 9
status: status/Planning
bookmark: false
mutable description: In an effort to show proof of concept for the CBDA synthase portion of the project, the GSU-iGem team is using horseradish peroxidase transformed into agrobacterium then producing transgenic tobacco plants.
mutable notes: It is intended to be put into pGAP vectors and pORE vectors for expression in Pichia Pastoris and Agrobacterium.
mutable provenance: synthesized by IDT
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1800002_sequence/1

BBa_K1640018
type: ComponentDefinition
persistent identity: BBa K1640018
display id: BBa_K1640018
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CHlM
creator: Meghan Cook
created: 2015-09-09T11:00:00Z
modified: 2016-10-20T07:15:09Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2057_
m user id: 7708
owner id: 28565
owning group id: 9
status: status/Planning
bookmark: true
mutable description: n/a
mutable notes: n/a
mutable provenance: n/a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: ChlM
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_K1640018_sequence/1

ChlM
type: SequenceAnnotation
persistent identity: annotation2474919
display id: annotation2474919
version: 1
top level: BBa_K1640018
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1640018/annotation2474919/range2474919/1

https://synbiohub.org/public/igem/BBa_K1640018/annotation2474919/range2474919/1
type: Range
persistent identity: range2474919
display id: range2474919
version: 1
direction: direction/forward
top level: BBa_K1640018
start: 20
end: 862
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2474918
display id: annotation2474918
version: 1
top level: BBa_K1640018
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1640018/annotation2474918/range2474918/1

https://synbiohub.org/public/igem/BBa_K1640018/annotation2474918/range2474918/1
type: Range
persistent identity: range2474918
display id: range2474918
version: 1
direction: direction/forward
top level: BBa_K1640018
start: 1
end: 11
orientation: inline

BBa_K1585312
type: ComponentDefinition
persistent identity: BBa K1585312
display id: BBa_K1585312
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0034.GlgC
creator: Michael Osthege, Sarah Lubjuhn, Laura Helleckes
created: 2015-08-24T11:00:00Z
modified: 2015-09-17T02:21:03Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2002_
m user id: 24474
owner id: 24505
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This is the translational unit of GlgC. It creates ADP-glucose from glucose-1-phosphate for glycogen synthesis.
mutable notes: -
mutable provenance: E. coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0034
component: BBa_K118016
sequence annotation: BBa_K118016
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K1585312_sequence/1

BBa_B0034
type: Component
persistent identity: component2437914
display id: component2437914
version: 1
top level: BBa_K1585312
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K118016
type: Component
persistent identity: component2437918
display id: component2437918
version: 1
top level: BBa_K1585312
access: public
definition: https://synbiohub.org/public/igem/BBa_K118016/1

BBa_K118016
type: SequenceAnnotation
persistent identity: annotation2437918
display id: annotation2437918
version: 1
top level: BBa_K1585312
location: https://synbiohub.org/public/igem/BBa_K1585312/annotation2437918/range2437918/1
component: BBa_K118016

https://synbiohub.org/public/igem/BBa_K1585312/annotation2437918/range2437918/1
type: Range
persistent identity: range2437918
display id: range2437918
version: 1
direction: direction/reverse
top level: BBa_K1585312
start: 19
end: 1317
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2437914
display id: annotation2437914
version: 1
top level: BBa_K1585312
location: https://synbiohub.org/public/igem/BBa_K1585312/annotation2437914/range2437914/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1585312/annotation2437914/range2437914/1
type: Range
persistent identity: range2437914
display id: range2437914
version: 1
direction: direction/reverse
top level: BBa_K1585312
start: 1
end: 12
orientation: inline

BBa_K1592003
type: ComponentDefinition
persistent identity: BBa K1592003
display id: BBa_K1592003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Mcfp3 with LIP2 prepro
creator: Shuyan Tang
created: 2015-09-05T11:00:00Z
modified: 2015-09-18T10:05:23Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2009_
m user id: 20267
owner id: 20267
owning group id: 9
status: status/Planning
bookmark: true
mutable description: Mcfp-3 is foot protein secreted from Mytilus californianus. The protein is of significance to the formation of byssus to help mussels permanently or temporarily tether to the surface of solid surface of reef or ship-body. This coherent substance shows the excellent adhesion performance with no substitute under water for sustaining the repeating wash of waves and having no toxicity. The signal tag, LIP2 prepro(BBa_K1592000), fused to Mcfp3 can lead the co-translational translocation of heterologous protein Mcfp-3 to be secreted out of the cell to achieve its function, during the progress of which the peptide will be deleted in the Golgi apparatus to eliminate the interference to the secreting protein. And hexahis-tag added between LIP2 prepro and Mcfp3 can therefore be used for protein purification.
mutable notes: none
mutable provenance: Synthesized by IDT,
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: BBa_K1592000
sequence annotation: BBa_K1592001
sequence annotation: LIP2 prepro
sequence annotation: BamHI
sequence annotation: his-tag
sequence annotation: Mcfp3
sequence: https://synbiohub.org/public/igem/BBa_K1592003_sequence/1

BBa_K1592000
type: SequenceAnnotation
persistent identity: annotation2444637
display id: annotation2444637
version: 1
top level: BBa_K1592003
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1592003/annotation2444637/range2444637/1

https://synbiohub.org/public/igem/BBa_K1592003/annotation2444637/range2444637/1
type: Range
persistent identity: range2444637
display id: range2444637
version: 1
direction: direction/forward
top level: BBa_K1592003
start: 1
end: 99
orientation: inline

BBa_K1592001
type: SequenceAnnotation
persistent identity: annotation2444643
display id: annotation2444643
version: 1
top level: BBa_K1592003
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1592003/annotation2444643/range2444643/1

https://synbiohub.org/public/igem/BBa_K1592003/annotation2444643/range2444643/1
type: Range
persistent identity: range2444643
display id: range2444643
version: 1
direction: direction/forward
top level: BBa_K1592003
start: 124
end: 357
orientation: inline

LIP2 prepro
type: SequenceAnnotation
persistent identity: annotation2444638
display id: annotation2444638
version: 1
top level: BBa_K1592003
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1592003/annotation2444638/range2444638/1

https://synbiohub.org/public/igem/BBa_K1592003/annotation2444638/range2444638/1
type: Range
persistent identity: range2444638
display id: range2444638
version: 1
direction: direction/forward
top level: BBa_K1592003
start: 1
end: 99
orientation: inline

BamHI
type: SequenceAnnotation
persistent identity: annotation2444639
display id: annotation2444639
version: 1
top level: BBa_K1592003
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1592003/annotation2444639/range2444639/1

https://synbiohub.org/public/igem/BBa_K1592003/annotation2444639/range2444639/1
type: Range
persistent identity: range2444639
display id: range2444639
version: 1
direction: direction/forward
top level: BBa_K1592003
start: 100
end: 105
orientation: inline

his-tag
type: SequenceAnnotation
persistent identity: annotation2444640
display id: annotation2444640
version: 1
top level: BBa_K1592003
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1592003/annotation2444640/range2444640/1

https://synbiohub.org/public/igem/BBa_K1592003/annotation2444640/range2444640/1
type: Range
persistent identity: range2444640
display id: range2444640
version: 1
direction: direction/forward
top level: BBa_K1592003
start: 106
end: 123
orientation: inline

Mcfp3
type: SequenceAnnotation
persistent identity: annotation2444642
display id: annotation2444642
version: 1
top level: BBa_K1592003
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1592003/annotation2444642/range2444642/1

https://synbiohub.org/public/igem/BBa_K1592003/annotation2444642/range2444642/1
type: Range
persistent identity: range2444642
display id: range2444642
version: 1
direction: direction/forward
top level: BBa_K1592003
start: 124
end: 357
orientation: inline

BBa_K1763000
type: ComponentDefinition
persistent identity: BBa K1763000
display id: BBa_K1763000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Apis mellifera (honeybee) silk fibroin 3
creator: Daniel Cancilla
created: 2015-05-20T11:00:00Z
modified: 2015-05-21T07:56:16Z
discontinued: false
dominant: false
group u list: _2185_
m user id: 18035
owner id: 18035
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This is the coding region for the honeybee silk protein #3 (will update more later)
mutable notes: Will update more later (mention that signal peptide has been removed)
mutable provenance: Apis mellifera genome. Reference Genbank FJ235090.1
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Honey bee silk coding region
sequence annotation: start codon
sequence: https://synbiohub.org/public/igem/BBa_K1763000_sequence/1

Honey bee silk coding region
type: SequenceAnnotation
persistent identity: annotation2432106
display id: annotation2432106
version: 1
top level: BBa_K1763000
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1763000/annotation2432106/range2432106/1

https://synbiohub.org/public/igem/BBa_K1763000/annotation2432106/range2432106/1
type: Range
persistent identity: range2432106
display id: range2432106
version: 1
direction: direction/forward
top level: BBa_K1763000
start: 1
end: 951
orientation: inline

start codon
type: SequenceAnnotation
persistent identity: annotation2432105
display id: annotation2432105
version: 1
top level: BBa_K1763000
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1763000/annotation2432105/range2432105/1

https://synbiohub.org/public/igem/BBa_K1763000/annotation2432105/range2432105/1
type: Range
persistent identity: range2432105
display id: range2432105
version: 1
direction: direction/forward
top level: BBa_K1763000
start: 1
end: 3
orientation: inline

HIC genera
type: ComponentDefinition
persistent identity: BBa K1602020
display id: BBa_K1602020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Inducible generator of Humicola insolens cutinase (HIC) with N-terminal His-Tag
creator: Sebastian Barthel, Steven Pilger, Jonas Sindlinger, Bianca Reisinger, Sven Jager
created: 2015-09-15T11:00:00Z
modified: 2015-09-26T03:05:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2019_
m user id: 12512
owner id: 22022
owning group id: 9
status: status/Planning
bookmark: true
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K1602018
component: BBa_I719005
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_I719005
sequence annotation: BBa_K1602018
sequence: https://synbiohub.org/public/igem/BBa_K1602020_sequence/1

BBa_K1602018
type: Component
persistent identity: component2462409
display id: component2462409
version: 1
top level: HIC genera
access: public
definition: https://synbiohub.org/public/igem/BBa_K1602018/1

BBa_I719005
type: Component
persistent identity: component2462406
display id: component2462406
version: 1
top level: HIC genera
access: public
definition: https://synbiohub.org/public/igem/BBa_I719005/1

BBa_B0034
type: Component
persistent identity: component2462408
display id: component2462408
version: 1
top level: HIC genera
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2462408
display id: annotation2462408
version: 1
top level: HIC genera
location: https://synbiohub.org/public/igem/BBa_K1602020/annotation2462408/range2462408/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1602020/annotation2462408/range2462408/1
type: Range
persistent identity: range2462408
display id: range2462408
version: 1
direction: direction/reverse
top level: HIC genera
start: 32
end: 43
orientation: inline

BBa_I719005
type: SequenceAnnotation
persistent identity: annotation2462406
display id: annotation2462406
version: 1
top level: HIC genera
location: https://synbiohub.org/public/igem/BBa_K1602020/annotation2462406/range2462406/1
component: BBa_I719005

https://synbiohub.org/public/igem/BBa_K1602020/annotation2462406/range2462406/1
type: Range
persistent identity: range2462406
display id: range2462406
version: 1
direction: direction/reverse
top level: HIC genera
start: 1
end: 23
orientation: inline

BBa_K1602018
type: SequenceAnnotation
persistent identity: annotation2462409
display id: annotation2462409
version: 1
top level: HIC genera
location: https://synbiohub.org/public/igem/BBa_K1602020/annotation2462409/range2462409/1
component: BBa_K1602018

https://synbiohub.org/public/igem/BBa_K1602020/annotation2462409/range2462409/1
type: Range
persistent identity: range2462409
display id: range2462409
version: 1
direction: direction/reverse
top level: HIC genera
start: 50
end: 652
orientation: inline

BBa_K1689014
type: ComponentDefinition
persistent identity: BBa K1689014
display id: BBa_K1689014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: dCas9-Clact
creator: Wei weijia
created: 2015-09-15T11:00:00Z
modified: 2015-09-27T01:31:12Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2107_
m user id: 26531
owner id: 26531
owning group id: 9
status: status/Planning
bookmark: false
mutable description: pSB1C3-6H-dCas9-Clact, C terminal segment of beta-lacatamse fused with dCas9, His tag allows for protein purification
mutable notes: delta alpha segment of LacZ fused with dCas9, His tag allows for protein purification
mutable provenance: E.coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: random spacer
sequence annotation: stop
sequence annotation: start
sequence annotation: dCas9
sequence annotation: C terminal of β-Lactamase
sequence annotation: 6??His tag
sequence annotation: GGGGS*2 Linker
sequence annotation: dCas9-Clact
sequence: https://synbiohub.org/public/igem/BBa_K1689014_sequence/1

random spacer
type: SequenceAnnotation
persistent identity: annotation2477777
display id: annotation2477777
version: 1
top level: BBa_K1689014
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1689014/annotation2477777/range2477777/1

https://synbiohub.org/public/igem/BBa_K1689014/annotation2477777/range2477777/1
type: Range
persistent identity: range2477777
display id: range2477777
version: 1
direction: direction/forward
top level: BBa_K1689014
start: 1
end: 33
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2477779
display id: annotation2477779
version: 1
top level: BBa_K1689014
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1689014/annotation2477779/range2477779/1

https://synbiohub.org/public/igem/BBa_K1689014/annotation2477779/range2477779/1
type: Range
persistent identity: range2477779
display id: range2477779
version: 1
direction: direction/forward
top level: BBa_K1689014
start: 4459
end: 4461
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2477778
display id: annotation2477778
version: 1
top level: BBa_K1689014
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1689014/annotation2477778/range2477778/1

https://synbiohub.org/public/igem/BBa_K1689014/annotation2477778/range2477778/1
type: Range
persistent identity: range2477778
display id: range2477778
version: 1
direction: direction/forward
top level: BBa_K1689014
start: 34
end: 36
orientation: inline

random spacer
type: SequenceAnnotation
persistent identity: annotation2477780
display id: annotation2477780
version: 1
top level: BBa_K1689014
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1689014/annotation2477780/range2477780/1

https://synbiohub.org/public/igem/BBa_K1689014/annotation2477780/range2477780/1
type: Range
persistent identity: range2477780
display id: range2477780
version: 1
direction: direction/forward
top level: BBa_K1689014
start: 4462
end: 4532
orientation: inline

dCas9
type: SequenceAnnotation
persistent identity: annotation2469006
display id: annotation2469006
version: 1
top level: BBa_K1689014
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1689014/annotation2469006/range2469006/1

https://synbiohub.org/public/igem/BBa_K1689014/annotation2469006/range2469006/1
type: Range
persistent identity: range2469006
display id: range2469006
version: 1
direction: direction/forward
top level: BBa_K1689014
start: 55
end: 4155
orientation: inline

C terminal of β-Lactamase
type: SequenceAnnotation
persistent identity: annotation2469012
display id: annotation2469012
version: 1
top level: BBa_K1689014
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1689014/annotation2469012/range2469012/1

https://synbiohub.org/public/igem/BBa_K1689014/annotation2469012/range2469012/1
type: Range
persistent identity: range2469012
display id: range2469012
version: 1
direction: direction/forward
top level: BBa_K1689014
start: 4186
end: 4458
orientation: inline

6??His tag
type: SequenceAnnotation
persistent identity: annotation2469003
display id: annotation2469003
version: 1
top level: BBa_K1689014
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1689014/annotation2469003/range2469003/1

https://synbiohub.org/public/igem/BBa_K1689014/annotation2469003/range2469003/1
type: Range
persistent identity: range2469003
display id: range2469003
version: 1
direction: direction/forward
top level: BBa_K1689014
start: 37
end: 54
orientation: inline

GGGGS*2 Linker
type: SequenceAnnotation
persistent identity: annotation2469010
display id: annotation2469010
version: 1
top level: BBa_K1689014
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1689014/annotation2469010/range2469010/1

https://synbiohub.org/public/igem/BBa_K1689014/annotation2469010/range2469010/1
type: Range
persistent identity: range2469010
display id: range2469010
version: 1
direction: direction/forward
top level: BBa_K1689014
start: 4156
end: 4185
orientation: inline

dCas9-Clact
type: SequenceAnnotation
persistent identity: annotation2470120
display id: annotation2470120
version: 1
top level: BBa_K1689014
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1689014/annotation2470120/range2470120/1

https://synbiohub.org/public/igem/BBa_K1689014/annotation2470120/range2470120/1
type: Range
persistent identity: range2470120
display id: range2470120
version: 1
direction: direction/forward
top level: BBa_K1689014
start: 34
end: 4461
orientation: inline

BBa_K1795013
type: ComponentDefinition
persistent identity: BBa K1795013
display id: BBa_K1795013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Origene Scrambled guide RNA under R0040
creator: Andrew D Halleran, Joseph L Maniaci
created: 2015-09-16T11:00:00Z
modified: 2015-09-17T09:46:57Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2220_
m user id: 27446
owner id: 27446
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This sgRNA sequence features a scrambled N20 sequence designed to serve as a negative control for dCas9 gene repression studies. The part is under the control of the promoter Bba_R0040. Promoter Bba_R0040 was chosen because it does not contain a PAM and therefore cannot be inadvertently repressed by dCas9 due to unintentional homology in the N20 sequence. Expression of this part can be repressed if TetR is expressed in the cell without Tetracycline present. If TetR and Tetracycline (or its analog aTc) are both present, the part will be expressed.
mutable provenance: The N20 sequence was used with Origene???s permission and was taken from Origene???s pCas Scramble (GE100003). The basic template for the sgRNA sequence was taken from the supplementary information of Qi LS, Larson MH, Gilbert LA, et al. Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression. Cell. 2013;152(5):1173-1183. doi:10.1016/j.cell.2013.02.022.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1795100
component: BBa_R0040
component: BBa_B0015
component: BBa_K1795113
sequence annotation: BBa_R0040
sequence annotation: BBa_K1795113
sequence annotation: BBa_K1795100
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K1795013_sequence/1

BBa_K1795100
type: Component
persistent identity: component2467977
display id: component2467977
version: 1
top level: BBa_K1795013
access: public
definition: https://synbiohub.org/public/igem/BBa_K1795100/1

BBa_R0040
type: Component
persistent identity: component2467971
display id: component2467971
version: 1
top level: BBa_K1795013
access: public
definition: p(tetR)

BBa_B0015
type: Component
persistent identity: component2467984
display id: component2467984
version: 1
top level: BBa_K1795013
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_K1795113
type: Component
persistent identity: component2467976
display id: component2467976
version: 1
top level: BBa_K1795013
access: public
definition: https://synbiohub.org/public/igem/BBa_K1795113/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2467971
display id: annotation2467971
version: 1
top level: BBa_K1795013
location: https://synbiohub.org/public/igem/BBa_K1795013/annotation2467971/range2467971/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K1795013/annotation2467971/range2467971/1
type: Range
persistent identity: range2467971
display id: range2467971
version: 1
direction: direction/forward
top level: BBa_K1795013
start: 1
end: 54
orientation: inline

BBa_K1795113
type: SequenceAnnotation
persistent identity: annotation2467976
display id: annotation2467976
version: 1
top level: BBa_K1795013
location: https://synbiohub.org/public/igem/BBa_K1795013/annotation2467976/range2467976/1
component: BBa_K1795113

https://synbiohub.org/public/igem/BBa_K1795013/annotation2467976/range2467976/1
type: Range
persistent identity: range2467976
display id: range2467976
version: 1
direction: direction/reverse
top level: BBa_K1795013
start: 55
end: 74
orientation: inline

BBa_K1795100
type: SequenceAnnotation
persistent identity: annotation2467977
display id: annotation2467977
version: 1
top level: BBa_K1795013
location: https://synbiohub.org/public/igem/BBa_K1795013/annotation2467977/range2467977/1
component: BBa_K1795100

https://synbiohub.org/public/igem/BBa_K1795013/annotation2467977/range2467977/1
type: Range
persistent identity: range2467977
display id: range2467977
version: 1
direction: direction/reverse
top level: BBa_K1795013
start: 75
end: 156
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2467984
display id: annotation2467984
version: 1
top level: BBa_K1795013
location: https://synbiohub.org/public/igem/BBa_K1795013/annotation2467984/range2467984/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1795013/annotation2467984/range2467984/1
type: Range
persistent identity: range2467984
display id: range2467984
version: 1
direction: direction/reverse
top level: BBa_K1795013
start: 157
end: 285
orientation: inline

Si-tag2+3
type: ComponentDefinition
persistent identity: BBa K1592011
display id: BBa_K1592011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LIP prepro + E. coli ribosomal protein L2 (61-273aa) + YLcwp3 Fusion
creator: Shuyan Tang
created: 2015-09-06T11:00:00Z
modified: 2015-09-18T09:34:57Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2009_
m user id: 20267
owner id: 20267
owning group id: 9
status: status/Planning
bookmark: true
mutable description: __NOTOC__ BBa_K1592008 short This is the cell display system of Yarrowia lipolytica, composed of LIP prepro, interest protein, and YLcwp3.LIP prepro is signal peptide used to secrete the interest protein out of the cell, and the YLcwp3 is the anchor domain binding the interest protein to the cell wall of yeast.We use this system to display silica-tag and test its binding characteristics. Here we use this system to display Silica-tag on cell wall of Yarrowia lipolytica to binding the silica. E.coli ribosomal protein L2 was found to bind tightly to silicon particles, which have surfaces that are oxidized to silica. This L2 silica-binding tag, called the 'Si-tag', can be used for one-step targeting of functional proteins on silica surfaces. The silica-binding domains of E. coli L2 was mapped to amino acids 1???60, 61-202 and 203???273, called Si-tag1, Si-tag2 and Si-tag3. We respectively test the silica-binding characteristics of this three regions and their combinations.
mutable notes: In order to replace the domains more conveniently, we respectivey added BamHI, SalI, NdeI between LIP2 prepro, E. coli ribosomal protein L2, GS linker and YLcwp3
mutable provenance: E. coli ribosomal protein L2 was synthesized by IDT, LIP2 prepro and YLcwp3 were cloning from the plasmid JMP62, and we fused this three sequences.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: GS Likner
sequence annotation: SalI
sequence annotation: Si-tag 23
sequence annotation: YLcwp3
sequence annotation: NdeI
sequence annotation: BamHI
sequence annotation: LIP2 prepro
sequence: https://synbiohub.org/public/igem/BBa_K1592011_sequence/1

GS Likner
type: SequenceAnnotation
persistent identity: annotation2456155
display id: annotation2456155
version: 1
top level: Si-tag2+3
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1592011/annotation2456155/range2456155/1

https://synbiohub.org/public/igem/BBa_K1592011/annotation2456155/range2456155/1
type: Range
persistent identity: range2456155
display id: range2456155
version: 1
direction: direction/forward
top level: Si-tag2+3
start: 754
end: 798
orientation: inline

SalI
type: SequenceAnnotation
persistent identity: annotation2456154
display id: annotation2456154
version: 1
top level: Si-tag2+3
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1592011/annotation2456154/range2456154/1

https://synbiohub.org/public/igem/BBa_K1592011/annotation2456154/range2456154/1
type: Range
persistent identity: range2456154
display id: range2456154
version: 1
direction: direction/forward
top level: Si-tag2+3
start: 748
end: 753
orientation: inline

Si-tag 23
type: SequenceAnnotation
persistent identity: annotation2456153
display id: annotation2456153
version: 1
top level: Si-tag2+3
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1592011/annotation2456153/range2456153/1

https://synbiohub.org/public/igem/BBa_K1592011/annotation2456153/range2456153/1
type: Range
persistent identity: range2456153
display id: range2456153
version: 1
direction: direction/forward
top level: Si-tag2+3
start: 106
end: 747
orientation: inline

YLcwp3
type: SequenceAnnotation
persistent identity: annotation2456157
display id: annotation2456157
version: 1
top level: Si-tag2+3
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1592011/annotation2456157/range2456157/1

https://synbiohub.org/public/igem/BBa_K1592011/annotation2456157/range2456157/1
type: Range
persistent identity: range2456157
display id: range2456157
version: 1
direction: direction/forward
top level: Si-tag2+3
start: 805
end: 1170
orientation: inline

NdeI
type: SequenceAnnotation
persistent identity: annotation2456156
display id: annotation2456156
version: 1
top level: Si-tag2+3
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1592011/annotation2456156/range2456156/1

https://synbiohub.org/public/igem/BBa_K1592011/annotation2456156/range2456156/1
type: Range
persistent identity: range2456156
display id: range2456156
version: 1
direction: direction/forward
top level: Si-tag2+3
start: 799
end: 804
orientation: inline

BamHI
type: SequenceAnnotation
persistent identity: annotation2456152
display id: annotation2456152
version: 1
top level: Si-tag2+3
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1592011/annotation2456152/range2456152/1

https://synbiohub.org/public/igem/BBa_K1592011/annotation2456152/range2456152/1
type: Range
persistent identity: range2456152
display id: range2456152
version: 1
direction: direction/forward
top level: Si-tag2+3
start: 100
end: 105
orientation: inline

LIP2 prepro
type: SequenceAnnotation
persistent identity: annotation2456151
display id: annotation2456151
version: 1
top level: Si-tag2+3
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1592011/annotation2456151/range2456151/1

https://synbiohub.org/public/igem/BBa_K1592011/annotation2456151/range2456151/1
type: Range
persistent identity: range2456151
display id: range2456151
version: 1
direction: direction/forward
top level: Si-tag2+3
start: 1
end: 99
orientation: inline

BBa_K1583204
type: ComponentDefinition
persistent identity: BBa K1583204
display id: BBa_K1583204
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Medium constitutive promoter + CsgB & CsgC
creator: Stefan Robert Marsden
created: 2015-09-03T11:00:00Z
modified: 2016-01-25T11:29:20Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2000_
m user id: 4206
owner id: 24463
owning group id: 9
status: status/Planning
bookmark: false
mutable description: CsgB functions as the anchor on the cell wall for curli nanowires. It triggers the aggregation of CsgA and leads to the formation of amyloid nanowires. CsgC is positively involved in the extracellular aggregation of CsgA into amyloid nanowires (curli assembly) in E.coli functioning as a chaperone for CsgA. Constructs with different promoter strenghts were designed to investigate the impact on nanowire self-assembly. Medium expression level.
mutable notes: We inserted non-coding scar sequences after the promoter and RBS to prevent missing initial codons during transcription/translation.
mutable provenance: The RBS and CsgC were synthesized from the genomic sequence of E.coli K-12 MG1655. The promoter is part of the constitutive promoter family made by John Anderson from iGEM Berkeley 2006.Non-coding scar sites were designed at random.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0034
component: BBa_K1583058
component: BBa_K1583001
component: BBa_K1583055
component: BBa_K1583054
component: BBa_K1583016
component: BBa_K1583053
component: BBa_J23118
sequence annotation: BBa_K1583054
sequence annotation: BBa_K1583053
sequence annotation: BBa_K1583055
sequence annotation: BBa_B0034
sequence annotation: BBa_K1583001
sequence annotation: BBa_K1583058
sequence annotation: BBa_K1583016
sequence annotation: BBa_J23118
sequence: https://synbiohub.org/public/igem/BBa_K1583204_sequence/1

BBa_B0034
type: Component
persistent identity: component2458765
display id: component2458765
version: 1
top level: BBa_K1583204
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1583058
type: Component
persistent identity: component2458760
display id: component2458760
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_K1583058/1

BBa_K1583001
type: Component
persistent identity: component2458767
display id: component2458767
version: 1
top level: BBa_K1583204
access: public
definition: https://synbiohub.org/public/igem/BBa_K1583001/1

BBa_K1583055
type: Component
persistent identity: component2458763
display id: component2458763
version: 1
top level: BBa_K1583204
access: public
definition: https://synbiohub.org/public/igem/BBa_K1583055/1

BBa_K1583054
type: Component
persistent identity: component2458766
display id: component2458766
version: 1
top level: BBa_K1583204
access: public
definition: https://synbiohub.org/public/igem/BBa_K1583054/1

BBa_K1583054
type: Component
persistent identity: component2458761
display id: component2458761
version: 1
top level: BBa_K1583204
access: public
definition: https://synbiohub.org/public/igem/BBa_K1583054/1

BBa_K1583016
type: Component
persistent identity: component2458762
display id: component2458762
version: 1
top level: BBa_K1583204
access: public
definition: https://synbiohub.org/public/igem/BBa_K1583016/1

BBa_K1583053
type: Component
persistent identity: component2458759
display id: component2458759
version: 1
top level: BBa_K1583204
access: public
definition: https://synbiohub.org/public/igem/BBa_K1583053/1

BBa_J23118
type: Component
persistent identity: component2458758
display id: component2458758
version: 1
top level: BBa_K1583204
access: public
definition: https://synbiohub.org/public/igem/BBa_J23118/1

BBa_K1583054
type: SequenceAnnotation
persistent identity: annotation2458766
display id: annotation2458766
version: 1
top level: BBa_K1583204
location: https://synbiohub.org/public/igem/BBa_K1583204/annotation2458766/range2458766/1
component: BBa_K1583054

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type: Range
persistent identity: range2458766
display id: range2458766
version: 1
direction: direction/reverse
top level: BBa_K1583204
start: 554
end: 559
orientation: inline

BBa_K1583053
type: SequenceAnnotation
persistent identity: annotation2458759
display id: annotation2458759
version: 1
top level: BBa_K1583204
location: https://synbiohub.org/public/igem/BBa_K1583204/annotation2458759/range2458759/1
component: BBa_K1583053

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type: Range
persistent identity: range2458759
display id: range2458759
version: 1
direction: direction/reverse
top level: BBa_K1583204
start: 36
end: 53
orientation: inline

BBa_K1583055
type: SequenceAnnotation
persistent identity: annotation2458763
display id: annotation2458763
version: 1
top level: BBa_K1583204
location: https://synbiohub.org/public/igem/BBa_K1583204/annotation2458763/range2458763/1
component: BBa_K1583055

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type: Range
persistent identity: range2458763
display id: range2458763
version: 1
direction: direction/reverse
top level: BBa_K1583204
start: 528
end: 541
orientation: inline

BBa_K1583054
type: SequenceAnnotation
persistent identity: annotation2458761
display id: annotation2458761
version: 1
top level: BBa_K1583204
location: https://synbiohub.org/public/igem/BBa_K1583204/annotation2458761/range2458761/1
component: BBa_K1583054

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type: Range
persistent identity: range2458761
display id: range2458761
version: 1
direction: direction/reverse
top level: BBa_K1583204
start: 66
end: 71
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2458765
display id: annotation2458765
version: 1
top level: BBa_K1583204
location: https://synbiohub.org/public/igem/BBa_K1583204/annotation2458765/range2458765/1
component: BBa_B0034

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type: Range
persistent identity: range2458765
display id: range2458765
version: 1
direction: direction/reverse
top level: BBa_K1583204
start: 542
end: 553
orientation: inline

BBa_K1583001
type: SequenceAnnotation
persistent identity: annotation2458767
display id: annotation2458767
version: 1
top level: BBa_K1583204
location: https://synbiohub.org/public/igem/BBa_K1583204/annotation2458767/range2458767/1
component: BBa_K1583001

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type: Range
persistent identity: range2458767
display id: range2458767
version: 1
direction: direction/reverse
top level: BBa_K1583204
start: 560
end: 892
orientation: inline

BBa_K1583058
type: SequenceAnnotation
persistent identity: annotation2458760
display id: annotation2458760
version: 1
top level: BBa_K1583204
location: https://synbiohub.org/public/igem/BBa_K1583204/annotation2458760/range2458760/1
component: BBa_K1583058

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type: Range
persistent identity: range2458760
display id: range2458760
version: 1
direction: direction/reverse
top level: BBa_K1583204
start: 54
end: 65
orientation: inline

BBa_K1583016
type: SequenceAnnotation
persistent identity: annotation2458762
display id: annotation2458762
version: 1
top level: BBa_K1583204
location: https://synbiohub.org/public/igem/BBa_K1583204/annotation2458762/range2458762/1
component: BBa_K1583016

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type: Range
persistent identity: range2458762
display id: range2458762
version: 1
direction: direction/reverse
top level: BBa_K1583204
start: 72
end: 527
orientation: inline

BBa_J23118
type: SequenceAnnotation
persistent identity: annotation2458758
display id: annotation2458758
version: 1
top level: BBa_K1583204
location: https://synbiohub.org/public/igem/BBa_K1583204/annotation2458758/range2458758/1
component: BBa_J23118

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type: Range
persistent identity: range2458758
display id: range2458758
version: 1
direction: direction/reverse
top level: BBa_K1583204
start: 1
end: 35
orientation: inline

BBa_K1585211
type: ComponentDefinition
persistent identity: BBa K1585211
display id: BBa_K1585211
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0034.HPS
creator: Laura Grabowski
created: 2015-08-24T11:00:00Z
modified: 2015-08-28T08:21:36Z
discontinued: false
dominant: false
group u list: _2002_
m user id: 19522
owner id: 24505
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This is the translational unit of 3-hexulose-6-phosphate synthase (HPS) from Bacillus methanolicus MGA3. It is codon optimized for E.coli.
mutable notes: RFC10 compatible
mutable provenance: Bacillus methanolicus MGA3
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1585201
component: BBa_B0034
sequence annotation: BBa_K1585201
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K1585211_sequence/1

BBa_K1585201
type: Component
persistent identity: component2439453
display id: component2439453
version: 1
top level: BBa_K1585211
access: public
definition: https://synbiohub.org/public/igem/BBa_K1585201/1

BBa_B0034
type: Component
persistent identity: component2439452
display id: component2439452
version: 1
top level: BBa_K1585211
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1585201
type: SequenceAnnotation
persistent identity: annotation2439453
display id: annotation2439453
version: 1
top level: BBa_K1585211
location: https://synbiohub.org/public/igem/BBa_K1585211/annotation2439453/range2439453/1
component: BBa_K1585201

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type: Range
persistent identity: range2439453
display id: range2439453
version: 1
direction: direction/reverse
top level: BBa_K1585211
start: 19
end: 657
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2439452
display id: annotation2439452
version: 1
top level: BBa_K1585211
location: https://synbiohub.org/public/igem/BBa_K1585211/annotation2439452/range2439452/1
component: BBa_B0034

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type: Range
persistent identity: range2439452
display id: range2439452
version: 1
direction: direction/reverse
top level: BBa_K1585211
start: 1
end: 12
orientation: inline

BBa_K1763426
type: ComponentDefinition
persistent identity: BBa K1763426
display id: BBa_K1763426
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: MaSp2-6(1C3)
creator: Vinson Lam
created: 2015-09-14T11:00:00Z
modified: 2015-09-15T08:38:24Z
discontinued: false
dominant: false
group u list: _2185_
m user id: 27136
owner id: 27136
owning group id: 9
status: status/Planning
bookmark: false
mutable description: MaSp2 6 mer in pSB1C3. No regulatory elements present.
mutable notes: no
mutable provenance: Nephila clavipes
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: M2-AB
sequence annotation: M2-BC
sequence annotation: 8-his tag
sequence annotation: M2-CA
sequence: https://synbiohub.org/public/igem/BBa_K1763426_sequence/1

M2-AB
type: SequenceAnnotation
persistent identity: annotation2463832
display id: annotation2463832
version: 1
top level: BBa_K1763426
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763426/annotation2463832/range2463832/1

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type: Range
persistent identity: range2463832
display id: range2463832
version: 1
direction: direction/forward
top level: BBa_K1763426
start: 36
end: 137
orientation: inline

M2-BC
type: SequenceAnnotation
persistent identity: annotation2463833
display id: annotation2463833
version: 1
top level: BBa_K1763426
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763426/annotation2463833/range2463833/1

https://synbiohub.org/public/igem/BBa_K1763426/annotation2463833/range2463833/1
type: Range
persistent identity: range2463833
display id: range2463833
version: 1
direction: direction/forward
top level: BBa_K1763426
start: 444
end: 545
orientation: inline

M2-AB
type: SequenceAnnotation
persistent identity: annotation2463835
display id: annotation2463835
version: 1
top level: BBa_K1763426
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763426/annotation2463835/range2463835/1

https://synbiohub.org/public/igem/BBa_K1763426/annotation2463835/range2463835/1
type: Range
persistent identity: range2463835
display id: range2463835
version: 1
direction: direction/forward
top level: BBa_K1763426
start: 342
end: 443
orientation: inline

M2-BC
type: SequenceAnnotation
persistent identity: annotation2463837
display id: annotation2463837
version: 1
top level: BBa_K1763426
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763426/annotation2463837/range2463837/1

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type: Range
persistent identity: range2463837
display id: range2463837
version: 1
direction: direction/forward
top level: BBa_K1763426
start: 138
end: 239
orientation: inline

8-his tag
type: SequenceAnnotation
persistent identity: annotation2463831
display id: annotation2463831
version: 1
top level: BBa_K1763426
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1763426/annotation2463831/range2463831/1

https://synbiohub.org/public/igem/BBa_K1763426/annotation2463831/range2463831/1
type: Range
persistent identity: range2463831
display id: range2463831
version: 1
direction: direction/forward
top level: BBa_K1763426
start: 4
end: 27
orientation: inline

M2-CA
type: SequenceAnnotation
persistent identity: annotation2463836
display id: annotation2463836
version: 1
top level: BBa_K1763426
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763426/annotation2463836/range2463836/1

https://synbiohub.org/public/igem/BBa_K1763426/annotation2463836/range2463836/1
type: Range
persistent identity: range2463836
display id: range2463836
version: 1
direction: direction/forward
top level: BBa_K1763426
start: 240
end: 341
orientation: inline

M2-CA
type: SequenceAnnotation
persistent identity: annotation2463834
display id: annotation2463834
version: 1
top level: BBa_K1763426
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763426/annotation2463834/range2463834/1

https://synbiohub.org/public/igem/BBa_K1763426/annotation2463834/range2463834/1
type: Range
persistent identity: range2463834
display id: range2463834
version: 1
direction: direction/forward
top level: BBa_K1763426
start: 546
end: 647
orientation: inline

BBa_K1789009
type: ComponentDefinition
persistent identity: BBa K1789009
display id: BBa_K1789009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TALE2
creator: Xinyuan Qiu
created: 2015-09-13T11:00:00Z
modified: 2015-09-14T07:20:22Z
discontinued: false
dominant: false
group u list: _2214_
m user id: 21134
owner id: 21134
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This is a engineered TAL effector that can bind with the DNA sequence 5'-GATAAACACCTTTC-3'
mutable notes: The part sequence should be able to correctly and efficiently bind with corresponding DNA, so we used bioinformatics tools to help us designing this sequence.
mutable provenance: This part is built and standardized by ourselves using GATEWAY technique.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: TALE2
sequence annotation: DNA Binding Domain
sequence: https://synbiohub.org/public/igem/BBa_K1789009_sequence/1

TALE2
type: SequenceAnnotation
persistent identity: annotation2454121
display id: annotation2454121
version: 1
top level: BBa_K1789009
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1789009/annotation2454121/range2454121/1

https://synbiohub.org/public/igem/BBa_K1789009/annotation2454121/range2454121/1
type: Range
persistent identity: range2454121
display id: range2454121
version: 1
direction: direction/forward
top level: BBa_K1789009
start: 1
end: 2088
orientation: inline

DNA Binding Domain
type: SequenceAnnotation
persistent identity: annotation2454120
display id: annotation2454120
version: 1
top level: BBa_K1789009
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1789009/annotation2454120/range2454120/1

https://synbiohub.org/public/igem/BBa_K1789009/annotation2454120/range2454120/1
type: Range
persistent identity: range2454120
display id: range2454120
version: 1
direction: direction/forward
top level: BBa_K1789009
start: 514
end: 1872
orientation: inline

BBa_K1699003
type: ComponentDefinition
persistent identity: BBa K1699003
display id: BBa_K1699003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: gRNA for dCas9-VP64 targeting synthetic activation promoter pMLPm
creator: Emil Ruvinov
created: 2015-08-08T11:00:00Z
modified: 2015-08-09T09:45:01Z
discontinued: false
dominant: false
group u list: _2117_
m user id: 24826
owner id: 24826
owning group id: 9
status: status/Planning
bookmark: false
mutable description: gRNA for dCas9-VP64 targeting synthetic activation promoter pMLPm
mutable notes: Design
mutable provenance: Source
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: gRNA for pMLPm
sequence annotation: Hammerhead ribozyme
sequence annotation: gRNA scaffold for SpCas9
sequence annotation: HDV ribozyme
sequence: https://synbiohub.org/public/igem/BBa_K1699003_sequence/1

gRNA for pMLPm
type: SequenceAnnotation
persistent identity: annotation2434600
display id: annotation2434600
version: 1
top level: BBa_K1699003
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1699003/annotation2434600/range2434600/1

https://synbiohub.org/public/igem/BBa_K1699003/annotation2434600/range2434600/1
type: Range
persistent identity: range2434600
display id: range2434600
version: 1
direction: direction/forward
top level: BBa_K1699003
start: 44
end: 63
orientation: inline

Hammerhead ribozyme
type: SequenceAnnotation
persistent identity: annotation2434599
display id: annotation2434599
version: 1
top level: BBa_K1699003
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1699003/annotation2434599/range2434599/1

https://synbiohub.org/public/igem/BBa_K1699003/annotation2434599/range2434599/1
type: Range
persistent identity: range2434599
display id: range2434599
version: 1
direction: direction/forward
top level: BBa_K1699003
start: 1
end: 43
orientation: inline

gRNA scaffold for SpCas9
type: SequenceAnnotation
persistent identity: annotation2434601
display id: annotation2434601
version: 1
top level: BBa_K1699003
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1699003/annotation2434601/range2434601/1

https://synbiohub.org/public/igem/BBa_K1699003/annotation2434601/range2434601/1
type: Range
persistent identity: range2434601
display id: range2434601
version: 1
direction: direction/forward
top level: BBa_K1699003
start: 64
end: 144
orientation: inline

HDV ribozyme
type: SequenceAnnotation
persistent identity: annotation2434602
display id: annotation2434602
version: 1
top level: BBa_K1699003
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1699003/annotation2434602/range2434602/1

https://synbiohub.org/public/igem/BBa_K1699003/annotation2434602/range2434602/1
type: Range
persistent identity: range2434602
display id: range2434602
version: 1
direction: direction/forward
top level: BBa_K1699003
start: 145
end: 210
orientation: inline

BBa_K1585213
type: ComponentDefinition
persistent identity: BBa K1585213
display id: BBa_K1585213
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0034.XPK
creator: Laura Grabowski
created: 2015-08-24T11:00:00Z
modified: 2015-08-28T08:37:34Z
discontinued: false
dominant: false
group u list: _2002_
m user id: 19522
owner id: 24505
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This is the translational unit for the phosphoketolase (XPK). It derives from Bifidobacterium adolescentis and is codon optimized for E. coli.
mutable notes: RFC10 compatible
mutable provenance: Bifidobacterium adolescentis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1585203
component: BBa_B0034
sequence annotation: BBa_K1585203
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K1585213_sequence/1

BBa_K1585203
type: Component
persistent identity: component2439488
display id: component2439488
version: 1
top level: BBa_K1585213
access: public
definition: https://synbiohub.org/public/igem/BBa_K1585203/1

BBa_B0034
type: Component
persistent identity: component2439486
display id: component2439486
version: 1
top level: BBa_K1585213
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1585203
type: SequenceAnnotation
persistent identity: annotation2439488
display id: annotation2439488
version: 1
top level: BBa_K1585213
location: https://synbiohub.org/public/igem/BBa_K1585213/annotation2439488/range2439488/1
component: BBa_K1585203

https://synbiohub.org/public/igem/BBa_K1585213/annotation2439488/range2439488/1
type: Range
persistent identity: range2439488
display id: range2439488
version: 1
direction: direction/reverse
top level: BBa_K1585213
start: 19
end: 2499
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2439486
display id: annotation2439486
version: 1
top level: BBa_K1585213
location: https://synbiohub.org/public/igem/BBa_K1585213/annotation2439486/range2439486/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1585213/annotation2439486/range2439486/1
type: Range
persistent identity: range2439486
display id: range2439486
version: 1
direction: direction/reverse
top level: BBa_K1585213
start: 1
end: 12
orientation: inline

tFba1
type: ComponentDefinition
persistent identity: BBa K1829004
display id: BBa_K1829004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: tFba1: yeast terminator
creator: Nicholas Suen, Hunter Collins
created: 2015-09-09T11:00:00Z
modified: 2015-09-18T06:14:38Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2255_
m user id: 27847
owner id: 27842
owning group id: 9
sample status: No part sequence
status: status/Planning
bookmark: false
mutable description: tENO2 is a terminator
mutable notes: cats
mutable provenance: cats
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence: https://synbiohub.org/public/igem/BBa_K1829004_sequence/1

Si-tag3
type: ComponentDefinition
persistent identity: BBa K1592009
display id: BBa_K1592009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LIP2 prepro + E. coli ribosomal protein L2 (203-273aa) + YLcwp3 Fusion
creator: Shuyan Tang
created: 2015-09-05T11:00:00Z
modified: 2015-09-18T09:27:28Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2009_
m user id: 20267
owner id: 20267
owning group id: 9
status: status/Planning
bookmark: true
mutable description: This is the cell display system of Yarrowia lipolytica, composed of LIP2 prepro, interest protein, and YLcwp3.LIP prepro is signal peptide used to secrete the interest protein out of the cell, and the YLcwp3 is the anchor domain binding the interest protein to the cell wall of yeast. We use this system to display silica-tag and test its binding characteristics. E.coli ribosomal protein L2 was found to bind tightly to silicon particles, which have surfaces that are oxidized to silica. This L2 silica-binding tag, called the 'Si-tag', can be used for one-step targeting of functional proteins on silica surfaces. The silica-binding domains of E. coli L2 was mapped to amino acids 1???60, 61-202 and 203???273, called Si-tag1, Si-tag2, Si-tag3. We respectively test the silica-binding characteristics of this three regions and their combinations.
mutable notes: In order to replace the domains more conveniently, we respectivey added BamHI, SalI, NdeI between LIP2 prepro, E. coli ribosomal protein L2, GS linker and YLcwp3.
mutable provenance: E. coli ribosomal protein L2 was synthesized by IDT, LIP2 prepro and YLcwp3 were cloning from the plasmid JMP62, and we fused this three sequences.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: GS Linker
sequence annotation: SalI
sequence annotation: YLcwp3
sequence annotation: Si-tag 3
sequence annotation: His6 tag
sequence annotation: NdeI
sequence annotation: LIP2 prepro
sequence annotation: BamHI
sequence: https://synbiohub.org/public/igem/BBa_K1592009_sequence/1

GS Linker
type: SequenceAnnotation
persistent identity: annotation2456126
display id: annotation2456126
version: 1
top level: Si-tag3
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1592009/annotation2456126/range2456126/1

https://synbiohub.org/public/igem/BBa_K1592009/annotation2456126/range2456126/1
type: Range
persistent identity: range2456126
display id: range2456126
version: 1
direction: direction/forward
top level: Si-tag3
start: 346
end: 372
orientation: inline

SalI
type: SequenceAnnotation
persistent identity: annotation2456125
display id: annotation2456125
version: 1
top level: Si-tag3
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1592009/annotation2456125/range2456125/1

https://synbiohub.org/public/igem/BBa_K1592009/annotation2456125/range2456125/1
type: Range
persistent identity: range2456125
display id: range2456125
version: 1
direction: direction/forward
top level: Si-tag3
start: 340
end: 345
orientation: inline

YLcwp3
type: SequenceAnnotation
persistent identity: annotation2456127
display id: annotation2456127
version: 1
top level: Si-tag3
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1592009/annotation2456127/range2456127/1

https://synbiohub.org/public/igem/BBa_K1592009/annotation2456127/range2456127/1
type: Range
persistent identity: range2456127
display id: range2456127
version: 1
direction: direction/forward
top level: Si-tag3
start: 379
end: 762
orientation: inline

Si-tag 3
type: SequenceAnnotation
persistent identity: annotation2456124
display id: annotation2456124
version: 1
top level: Si-tag3
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1592009/annotation2456124/range2456124/1

https://synbiohub.org/public/igem/BBa_K1592009/annotation2456124/range2456124/1
type: Range
persistent identity: range2456124
display id: range2456124
version: 1
direction: direction/forward
top level: Si-tag3
start: 124
end: 339
orientation: inline

His6 tag
type: SequenceAnnotation
persistent identity: annotation2456123
display id: annotation2456123
version: 1
top level: Si-tag3
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1592009/annotation2456123/range2456123/1

https://synbiohub.org/public/igem/BBa_K1592009/annotation2456123/range2456123/1
type: Range
persistent identity: range2456123
display id: range2456123
version: 1
direction: direction/forward
top level: Si-tag3
start: 106
end: 123
orientation: inline

NdeI
type: SequenceAnnotation
persistent identity: annotation2456187
display id: annotation2456187
version: 1
top level: Si-tag3
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1592009/annotation2456187/range2456187/1

https://synbiohub.org/public/igem/BBa_K1592009/annotation2456187/range2456187/1
type: Range
persistent identity: range2456187
display id: range2456187
version: 1
direction: direction/forward
top level: Si-tag3
start: 373
end: 378
orientation: inline

LIP2 prepro
type: SequenceAnnotation
persistent identity: annotation2456121
display id: annotation2456121
version: 1
top level: Si-tag3
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1592009/annotation2456121/range2456121/1

https://synbiohub.org/public/igem/BBa_K1592009/annotation2456121/range2456121/1
type: Range
persistent identity: range2456121
display id: range2456121
version: 1
direction: direction/forward
top level: Si-tag3
start: 1
end: 99
orientation: inline

BamHI
type: SequenceAnnotation
persistent identity: annotation2456122
display id: annotation2456122
version: 1
top level: Si-tag3
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1592009/annotation2456122/range2456122/1

https://synbiohub.org/public/igem/BBa_K1592009/annotation2456122/range2456122/1
type: Range
persistent identity: range2456122
display id: range2456122
version: 1
direction: direction/forward
top level: Si-tag3
start: 100
end: 105
orientation: inline

BBa_K1799002
type: ComponentDefinition
persistent identity: BBa K1799002
display id: BBa_K1799002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LsrR
creator: Miranda Halle
created: 2015-08-06T11:00:00Z
modified: 2015-09-18T01:12:11Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2224_
m user id: 9151
owner id: 19613
owning group id: 9
status: status/Planning
bookmark: true
mutable description: Gene for the repressor LsrR, present in the E. coli LsrRK operon. Represses the pLsrA promoter, as well as its own promoter, pLsrR
mutable notes: None.
mutable provenance: This part was synthesized from E. coli genomic data retrieved via EcoGene: http://www.ecogene.org/gene/EG13805
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1799002_sequence/1

BBa_K1758323
type: ComponentDefinition
persistent identity: BBa K1758323
display id: BBa_K1758323
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: UTR-sfGFP under control of Copper responsive promoter
creator: Team Bielefeld-CeBiTec 2015
created: 2015-08-28T11:00:00Z
modified: 2016-01-06T03:42:20Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2180_
m user id: 4206
owner id: 26630
owning group id: 9
status: status/Planning
bookmark: false
mutable description: copper responsive promoter with 5?? untranslated region and sfGFP for detection
mutable notes: ...
mutable provenance: copper responsive promoter of ????E.coli`` with sfGFP for detection
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: sfGFP
sequence annotation: T7-term
sequence annotation: 5??UTR
sequence annotation: CopAp
sequence annotation: T1
sequence: https://synbiohub.org/public/igem/BBa_K1758323_sequence/1

sfGFP
type: SequenceAnnotation
persistent identity: annotation2439963
display id: annotation2439963
version: 1
top level: BBa_K1758323
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1758323/annotation2439963/range2439963/1

https://synbiohub.org/public/igem/BBa_K1758323/annotation2439963/range2439963/1
type: Range
persistent identity: range2439963
display id: range2439963
version: 1
direction: direction/forward
top level: BBa_K1758323
start: 136
end: 855
orientation: inline

T7-term
type: SequenceAnnotation
persistent identity: annotation2439965
display id: annotation2439965
version: 1
top level: BBa_K1758323
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1758323/annotation2439965/range2439965/1

https://synbiohub.org/public/igem/BBa_K1758323/annotation2439965/range2439965/1
type: Range
persistent identity: range2439965
display id: range2439965
version: 1
direction: direction/forward
top level: BBa_K1758323
start: 952
end: 992
orientation: inline

5??UTR
type: SequenceAnnotation
persistent identity: annotation2439962
display id: annotation2439962
version: 1
top level: BBa_K1758323
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1758323/annotation2439962/range2439962/1

https://synbiohub.org/public/igem/BBa_K1758323/annotation2439962/range2439962/1
type: Range
persistent identity: range2439962
display id: range2439962
version: 1
direction: direction/forward
top level: BBa_K1758323
start: 90
end: 135
orientation: inline

CopAp
type: SequenceAnnotation
persistent identity: annotation2439961
display id: annotation2439961
version: 1
top level: BBa_K1758323
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1758323/annotation2439961/range2439961/1

https://synbiohub.org/public/igem/BBa_K1758323/annotation2439961/range2439961/1
type: Range
persistent identity: range2439961
display id: range2439961
version: 1
direction: direction/forward
top level: BBa_K1758323
start: 1
end: 83
orientation: inline

T1
type: SequenceAnnotation
persistent identity: annotation2439964
display id: annotation2439964
version: 1
top level: BBa_K1758323
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1758323/annotation2439964/range2439964/1

https://synbiohub.org/public/igem/BBa_K1758323/annotation2439964/range2439964/1
type: Range
persistent identity: range2439964
display id: range2439964
version: 1
direction: direction/forward
top level: BBa_K1758323
start: 864
end: 943
orientation: inline

BBa_K1699004
type: ComponentDefinition
persistent identity: BBa K1699004
display id: BBa_K1699004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: gRNA for SaCas9 targeting human ubiquitin B gene under U6 promoter
creator: Emil Ruvinov
created: 2015-08-08T11:00:00Z
modified: 2015-09-13T06:26:36Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2117_
m user id: 24826
owner id: 24826
owning group id: 9
status: status/Planning
bookmark: false
mutable description: gRNA for SaCas9 targeting ubiquitin B under U6 promoter
mutable notes: Design
mutable provenance: Source
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: gRNA-UBB-Exon 2
sequence annotation: gRNA scaffold for SaCas9
sequence annotation: Human U6 promoter
sequence: https://synbiohub.org/public/igem/BBa_K1699004_sequence/1

gRNA-UBB-Exon 2
type: SequenceAnnotation
persistent identity: annotation2434619
display id: annotation2434619
version: 1
top level: BBa_K1699004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1699004/annotation2434619/range2434619/1

https://synbiohub.org/public/igem/BBa_K1699004/annotation2434619/range2434619/1
type: Range
persistent identity: range2434619
display id: range2434619
version: 1
direction: direction/forward
top level: BBa_K1699004
start: 257
end: 278
orientation: inline

gRNA scaffold for SaCas9
type: SequenceAnnotation
persistent identity: annotation2434620
display id: annotation2434620
version: 1
top level: BBa_K1699004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1699004/annotation2434620/range2434620/1

https://synbiohub.org/public/igem/BBa_K1699004/annotation2434620/range2434620/1
type: Range
persistent identity: range2434620
display id: range2434620
version: 1
direction: direction/forward
top level: BBa_K1699004
start: 279
end: 359
orientation: inline

Human U6 promoter
type: SequenceAnnotation
persistent identity: annotation2434618
display id: annotation2434618
version: 1
top level: BBa_K1699004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1699004/annotation2434618/range2434618/1

https://synbiohub.org/public/igem/BBa_K1699004/annotation2434618/range2434618/1
type: Range
persistent identity: range2434618
display id: range2434618
version: 1
direction: direction/forward
top level: BBa_K1699004
start: 1
end: 249
orientation: inline

BBa_K1758106
type: ComponentDefinition
persistent identity: BBa K1758106
display id: BBa_K1758106
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Translation enhancing 5-UTR + mRFP under control of T7 promoter
creator: Team Bielefeld-CeBiTec 2015
created: 2015-08-26T11:00:00Z
modified: 2015-09-18T06:40:02Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2180_
m user id: 26563
owner id: 26563
owning group id: 9
status: status/Planning
bookmark: false
mutable description: mRFP generator ([http://parts.igem.org/Part:BBa_K516030 BBa_K516030]) under control of the T7 promoter ([http://parts.igem.org/Part:BBa_I719005 BBa_I719005]). The part includes a translation enhancing sequence, 5'-UTR, which improves the binding of the mRNA to the ribosome ([http://www.ncbi.nlm.nih.gov/pubmed/2676996 Olins et al. 1989]). The 5'-UTR sequence contains a spacer of 10 adenine bases which has been shown to further enhance translation efficiency ([http://www.ncbi.nlm.nih.gov/pubmed/23927491 Takahashi et al. 2013]).
mutable notes: See [http://parts.igem.org/Part:BBa_K1758102:Design BBa_K1758102]
mutable provenance: This part was obtained via Gibson primers that bind on [http://parts.igem.org/Part:BBa_K1758105 BBa_K1758105].
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K283035
component: BBa_I719005
component: BBa_K1758100
sequence annotation: BBa_K283035
sequence annotation: BBa_I719005
sequence annotation: BBa_K1758100
sequence: https://synbiohub.org/public/igem/BBa_K1758106_sequence/1

BBa_K283035
type: Component
persistent identity: component2439116
display id: component2439116
version: 1
top level: BBa_K1758106
access: public
definition: https://synbiohub.org/public/igem/BBa_K283035/1

BBa_I719005
type: Component
persistent identity: component2439103
display id: component2439103
version: 1
top level: BBa_K1758106
access: public
definition: https://synbiohub.org/public/igem/BBa_I719005/1

BBa_K1758100
type: Component
persistent identity: component2439106
display id: component2439106
version: 1
top level: BBa_K1758106
access: public
definition: https://synbiohub.org/public/igem/BBa_K1758100/1

BBa_K283035
type: SequenceAnnotation
persistent identity: annotation2439116
display id: annotation2439116
version: 1
top level: BBa_K1758106
location: https://synbiohub.org/public/igem/BBa_K1758106/annotation2439116/range2439116/1
component: BBa_K283035

https://synbiohub.org/public/igem/BBa_K1758106/annotation2439116/range2439116/1
type: Range
persistent identity: range2439116
display id: range2439116
version: 1
direction: direction/reverse
top level: BBa_K1758106
start: 70
end: 912
orientation: inline

BBa_I719005
type: SequenceAnnotation
persistent identity: annotation2439103
display id: annotation2439103
version: 1
top level: BBa_K1758106
location: https://synbiohub.org/public/igem/BBa_K1758106/annotation2439103/range2439103/1
component: BBa_I719005

https://synbiohub.org/public/igem/BBa_K1758106/annotation2439103/range2439103/1
type: Range
persistent identity: range2439103
display id: range2439103
version: 1
direction: direction/reverse
top level: BBa_K1758106
start: 1
end: 23
orientation: inline

BBa_K1758100
type: SequenceAnnotation
persistent identity: annotation2439106
display id: annotation2439106
version: 1
top level: BBa_K1758106
location: https://synbiohub.org/public/igem/BBa_K1758106/annotation2439106/range2439106/1
component: BBa_K1758100

https://synbiohub.org/public/igem/BBa_K1758106/annotation2439106/range2439106/1
type: Range
persistent identity: range2439106
display id: range2439106
version: 1
direction: direction/reverse
top level: BBa_K1758106
start: 24
end: 69
orientation: inline

BBa_K1758333
type: ComponentDefinition
persistent identity: BBa K1758333
display id: BBa_K1758333
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lead repressor under control of constitutive promoter and strong RBS and lead responsive promoter wi
creator: Team Bielefeld-CeBiTec 2015
created: 2015-08-29T11:00:00Z
modified: 2016-01-06T03:41:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2180_
m user id: 4206
owner id: 26630
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Repressor for lead responsive promoter under the control of konstitutive promoter and lead responsive promoter with 5??untranslated region and sfGFP for detection. As a lead biosensor
mutable notes: ??...
mutable provenance: genomic sequence out of ????Cupriavidus metallidurans???? CH34
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1758330
component: BBa_K1758332
sequence annotation: BBa_K1758332
sequence annotation: BBa_K1758330
sequence: https://synbiohub.org/public/igem/BBa_K1758333_sequence/1

BBa_K1758330
type: Component
persistent identity: component2440122
display id: component2440122
version: 1
top level: BBa_K1758333
access: public
definition: BBa_K1758330

BBa_K1758332
type: Component
persistent identity: component2440128
display id: component2440128
version: 1
top level: BBa_K1758333
access: public
definition: https://synbiohub.org/public/igem/BBa_K1758332/1

BBa_K1758332
type: SequenceAnnotation
persistent identity: annotation2440128
display id: annotation2440128
version: 1
top level: BBa_K1758333
location: https://synbiohub.org/public/igem/BBa_K1758333/annotation2440128/range2440128/1
component: BBa_K1758332

https://synbiohub.org/public/igem/BBa_K1758333/annotation2440128/range2440128/1
type: Range
persistent identity: range2440128
display id: range2440128
version: 1
direction: direction/reverse
top level: BBa_K1758333
start: 508
end: 1484
orientation: inline

BBa_K1758330
type: SequenceAnnotation
persistent identity: annotation2440122
display id: annotation2440122
version: 1
top level: BBa_K1758333
location: https://synbiohub.org/public/igem/BBa_K1758333/annotation2440122/range2440122/1
component: BBa_K1758330

https://synbiohub.org/public/igem/BBa_K1758333/annotation2440122/range2440122/1
type: Range
persistent identity: range2440122
display id: range2440122
version: 1
direction: direction/reverse
top level: BBa_K1758333
start: 1
end: 499
orientation: inline

BBa_K1825005
type: ComponentDefinition
persistent identity: BBa K1825005
display id: BBa_K1825005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: nptII
creator: Jonathan Asmund Arnesen
created: 2015-09-02T11:00:00Z
modified: 2015-12-16T12:17:11Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2251_
m user id: 4206
owner id: 28047
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Neomycin Phosphotransferase, for a composite biobrick.
mutable notes: none
mutable provenance: From a vector.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1825005_sequence/1

BBa_K1602055
type: ComponentDefinition
persistent identity: BBa K1602055
display id: BBa_K1602055
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: araCpBad-GFP
creator: Christian Sator,Stefan Zens, Max Zander, Benedikt Spannenkrebs, Sebastian Jaeger
created: 2015-09-15T11:00:00Z
modified: 2015-09-25T04:55:36Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2019_
m user id: 23028
owner id: 26220
owning group id: 9
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0034
component: BBa_K808000
component: BBa_E0040
sequence annotation: BBa_K808000
sequence annotation: BBa_B0034
sequence annotation: BBa_E0040
sequence: https://synbiohub.org/public/igem/BBa_K1602055_sequence/1

BBa_B0034
type: Component
persistent identity: component2462750
display id: component2462750
version: 1
top level: BBa_K1602055
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K808000
type: Component
persistent identity: component2462748
display id: component2462748
version: 1
top level: BBa_K1602055
access: public
definition: https://synbiohub.org/public/igem/BBa_K808000/1

BBa_E0040
type: Component
persistent identity: component2462752
display id: component2462752
version: 1
top level: BBa_K1602055
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_K808000
type: SequenceAnnotation
persistent identity: annotation2462748
display id: annotation2462748
version: 1
top level: BBa_K1602055
location: https://synbiohub.org/public/igem/BBa_K1602055/annotation2462748/range2462748/1
component: BBa_K808000

https://synbiohub.org/public/igem/BBa_K1602055/annotation2462748/range2462748/1
type: Range
persistent identity: range2462748
display id: range2462748
version: 1
direction: direction/reverse
top level: BBa_K1602055
start: 1
end: 1209
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2462750
display id: annotation2462750
version: 1
top level: BBa_K1602055
location: https://synbiohub.org/public/igem/BBa_K1602055/annotation2462750/range2462750/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1602055/annotation2462750/range2462750/1
type: Range
persistent identity: range2462750
display id: range2462750
version: 1
direction: direction/reverse
top level: BBa_K1602055
start: 1218
end: 1229
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2462752
display id: annotation2462752
version: 1
top level: BBa_K1602055
location: https://synbiohub.org/public/igem/BBa_K1602055/annotation2462752/range2462752/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K1602055/annotation2462752/range2462752/1
type: Range
persistent identity: range2462752
display id: range2462752
version: 1
direction: direction/reverse
top level: BBa_K1602055
start: 1236
end: 1955
orientation: inline

BBa_K1692005
type: ComponentDefinition
persistent identity: BBa K1692005
display id: BBa_K1692005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: UbiX
creator: Daniel Kunin
created: 2015-08-02T11:00:00Z
modified: 2015-08-03T12:24:31Z
discontinued: false
dominant: false
group u list: _2110_
m user id: 26209
owner id: 26209
owning group id: 9
status: status/Planning
bookmark: false
mutable description: UbiX is a flavin prenyltransferase required in ubiquinone synthesis in Escherichia coli.
mutable notes: No considerations
mutable provenance: Escherichia coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1692005_sequence/1

BBa_K1692023
type: ComponentDefinition
persistent identity: BBa K1692023
display id: BBa_K1692023
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ptet + Luxl
creator: Thai Nguyen
created: 2015-09-16T11:00:00Z
modified: 2015-09-22T11:50:15Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2110_
m user id: 28204
owner id: 28204
owning group id: 9
status: status/Planning
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_R0040
component: BBa_K805016
sequence annotation: BBa_K805016
sequence annotation: BBa_R0040
sequence: https://synbiohub.org/public/igem/BBa_K1692023_sequence/1

BBa_R0040
type: Component
persistent identity: component2476306
display id: component2476306
version: 1
top level: BBa_K1692023
access: public
definition: p(tetR)

BBa_K805016
type: Component
persistent identity: component2476324
display id: component2476324
version: 1
top level: BBa_K1692023
access: public
definition: https://synbiohub.org/public/igem/BBa_K805016/1

BBa_K805016
type: SequenceAnnotation
persistent identity: annotation2476324
display id: annotation2476324
version: 1
top level: BBa_K1692023
location: https://synbiohub.org/public/igem/BBa_K1692023/annotation2476324/range2476324/1
component: BBa_K805016

https://synbiohub.org/public/igem/BBa_K1692023/annotation2476324/range2476324/1
type: Range
persistent identity: range2476324
display id: range2476324
version: 1
direction: direction/reverse
top level: BBa_K1692023
start: 63
end: 860
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2476306
display id: annotation2476306
version: 1
top level: BBa_K1692023
location: https://synbiohub.org/public/igem/BBa_K1692023/annotation2476306/range2476306/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K1692023/annotation2476306/range2476306/1
type: Range
persistent identity: range2476306
display id: range2476306
version: 1
direction: direction/forward
top level: BBa_K1692023
start: 1
end: 54
orientation: inline

BBa_K1758335
type: ComponentDefinition
persistent identity: BBa K1758335
display id: BBa_K1758335
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: UTR-sfGFP controlled by T7-promoter and lead responsive promoter
creator: Team Bielefeld-CeBiTec 2015
created: 2015-09-08T11:00:00Z
modified: 2016-01-06T03:41:29Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2180_
m user id: 4206
owner id: 26630
owning group id: 9
status: status/Planning
bookmark: false
mutable description: lead induceble promoter under the control of T7 Promoter with mRFP for detection
mutable notes: ...
mutable provenance: This part consists of T7 promoter lead induceble Promoter PbrA from ''Cupriavidus metallidurans CH34'' and mRFP for detection
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1758331
component: BBa_I719005
sequence annotation: BBa_I719005
sequence annotation: BBa_K1758331
sequence: https://synbiohub.org/public/igem/BBa_K1758335_sequence/1

BBa_K1758331
type: Component
persistent identity: component2447790
display id: component2447790
version: 1
top level: BBa_K1758335
access: public
definition: BBa_K1758331

BBa_I719005
type: Component
persistent identity: component2447787
display id: component2447787
version: 1
top level: BBa_K1758335
access: public
definition: https://synbiohub.org/public/igem/BBa_I719005/1

BBa_I719005
type: SequenceAnnotation
persistent identity: annotation2447787
display id: annotation2447787
version: 1
top level: BBa_K1758335
location: https://synbiohub.org/public/igem/BBa_K1758335/annotation2447787/range2447787/1
component: BBa_I719005

https://synbiohub.org/public/igem/BBa_K1758335/annotation2447787/range2447787/1
type: Range
persistent identity: range2447787
display id: range2447787
version: 1
direction: direction/reverse
top level: BBa_K1758335
start: 1
end: 23
orientation: inline

BBa_K1758331
type: SequenceAnnotation
persistent identity: annotation2447790
display id: annotation2447790
version: 1
top level: BBa_K1758335
location: https://synbiohub.org/public/igem/BBa_K1758335/annotation2447790/range2447790/1
component: BBa_K1758331

https://synbiohub.org/public/igem/BBa_K1758335/annotation2447790/range2447790/1
type: Range
persistent identity: range2447790
display id: range2447790
version: 1
direction: direction/reverse
top level: BBa_K1758335
start: 32
end: 786
orientation: inline

RBS-xylC
type: ComponentDefinition
persistent identity: BBa K1602001
display id: BBa_K1602001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0034-xylC
creator: Jonas Sindlinger, Leon Kraus, Jonathan Pletzer-Zelgert, Elena Tonchevska, Sebastian Holzner, Mirjam Haun, Sebastian Barthel
created: 2015-08-27T11:00:00Z
modified: 2015-09-18T12:11:36Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2019_
m user id: 22022
owner id: 25989
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Blank
mutable notes: Blank
mutable provenance: E.coli TOP10
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_B0034
component: BBa_K1602010
sequence annotation: BBa_K1602010
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K1602001_sequence/1

BBa_B0034
type: Component
persistent identity: component2463052
display id: component2463052
version: 1
top level: RBS-xylC
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1602010
type: Component
persistent identity: component2463053
display id: component2463053
version: 1
top level: RBS-xylC
access: public
definition: BBa_K1602010

BBa_K1602010
type: SequenceAnnotation
persistent identity: annotation2463053
display id: annotation2463053
version: 1
top level: RBS-xylC
location: https://synbiohub.org/public/igem/BBa_K1602001/annotation2463053/range2463053/1
component: BBa_K1602010

https://synbiohub.org/public/igem/BBa_K1602001/annotation2463053/range2463053/1
type: Range
persistent identity: range2463053
display id: range2463053
version: 1
direction: direction/reverse
top level: RBS-xylC
start: 19
end: 888
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2463052
display id: annotation2463052
version: 1
top level: RBS-xylC
location: https://synbiohub.org/public/igem/BBa_K1602001/annotation2463052/range2463052/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1602001/annotation2463052/range2463052/1
type: Range
persistent identity: range2463052
display id: range2463052
version: 1
direction: direction/reverse
top level: RBS-xylC
start: 1
end: 12
orientation: inline

YLcwp3
type: ComponentDefinition
persistent identity: BBa K1592002
display id: BBa_K1592002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Yarrowia lipolytica cell wall protein 3
creator: Shuyan Tang
created: 2015-08-30T11:00:00Z
modified: 2015-09-18T09:24:05Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2009_
m user id: 20267
owner id: 20267
owning group id: 9
status: status/Planning
bookmark: false
mutable description: The covalently bound GPI-anchored cell wall protein of Y. lipolytica Ylcwp1 has been isolated and characterized (Jaafar and Zueco 2004). The nucleotide sequence encoding 110 amino acids of the Ylcwp1 C terminus has been utilized for the construction of the cell surface display vector in Y. lipolytica (Yue et al. 2008). This system was successfully used to immobilize green fluorescent protein (Yue et al. 2008). The ability of five new putative covalently linked cell wall proteins of Y. lipolytica -Ylcwp1, Ylcwp2, Ylcwp3, Ylcwp4, Ylcwp5, was tested and the highest cell-bound lipase value was obtained with the anchor domains Ylcwp3 (Evgeniya et al. 2011), so we utilized the YLcwp3 for the cell surface display in Y. lipolytica.
mutable notes: No
mutable provenance: The source of the part will be its sequence which was retrieved from GenBank, and altered some regions by our lab.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1592002_sequence/1

BBa_K1758311
type: ComponentDefinition
persistent identity: BBa K1758311
display id: BBa_K1758311
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: mRFP under control of the chromium responsive promoter
creator: Team Bielefeld-CeBiTec 2015
created: 2015-08-29T11:00:00Z
modified: 2015-09-20T08:45:00Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2180_
m user id: 26931
owner id: 26630
owning group id: 9
status: status/Planning
bookmark: false
mutable description: chromium responsive Promoter with RFP for detecition
mutable notes: ...
mutable provenance: chromium VI responsive promoter of ????Ochrobactrum tritici???? 5bvl1
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: ChrP
sequence annotation: mRFP
sequence: https://synbiohub.org/public/igem/BBa_K1758311_sequence/1

ChrP
type: SequenceAnnotation
persistent identity: annotation2440065
display id: annotation2440065
version: 1
top level: BBa_K1758311
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1758311/annotation2440065/range2440065/1

https://synbiohub.org/public/igem/BBa_K1758311/annotation2440065/range2440065/1
type: Range
persistent identity: range2440065
display id: range2440065
version: 1
direction: direction/forward
top level: BBa_K1758311
start: 1
end: 165
orientation: inline

mRFP
type: SequenceAnnotation
persistent identity: annotation2440064
display id: annotation2440064
version: 1
top level: BBa_K1758311
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1758311/annotation2440064/range2440064/1

https://synbiohub.org/public/igem/BBa_K1758311/annotation2440064/range2440064/1
type: Range
persistent identity: range2440064
display id: range2440064
version: 1
direction: direction/forward
top level: BBa_K1758311
start: 171
end: 848
orientation: inline

BBa_K1789003
type: ComponentDefinition
persistent identity: BBa K1789003
display id: BBa_K1789003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP1(with termination codon)
creator: Xinyuan Qiu
created: 2015-09-13T11:00:00Z
modified: 2016-01-25T01:04:29Z
discontinued: false
dominant: false
group u list: _2214_
m user id: 4206
owner id: 21134
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This part is the Amino Half of GFP with termination codon
mutable notes: Because of the need of our project, we added a termination codon when designing the PCR.
mutable provenance: This part is gained from part BBa_I715019. we added the termination codon when designing the PCR.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: GFP1
sequence annotation: termination codon
sequence: https://synbiohub.org/public/igem/BBa_K1789003_sequence/1

GFP1
type: SequenceAnnotation
persistent identity: annotation2454065
display id: annotation2454065
version: 1
top level: BBa_K1789003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1789003/annotation2454065/range2454065/1

https://synbiohub.org/public/igem/BBa_K1789003/annotation2454065/range2454065/1
type: Range
persistent identity: range2454065
display id: range2454065
version: 1
direction: direction/forward
top level: BBa_K1789003
start: 1
end: 471
orientation: inline

termination codon
type: SequenceAnnotation
persistent identity: annotation2454066
display id: annotation2454066
version: 1
top level: BBa_K1789003
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1789003/annotation2454066/range2454066/1

https://synbiohub.org/public/igem/BBa_K1789003/annotation2454066/range2454066/1
type: Range
persistent identity: range2454066
display id: range2454066
version: 1
direction: direction/forward
top level: BBa_K1789003
start: 472
end: 474
orientation: inline

Si-tag2
type: ComponentDefinition
persistent identity: BBa K1592008
display id: BBa_K1592008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LIP prepro + E. coli ribosomal protein L2 (61-202aa) + YLcwp3 Fusion
creator: Shuyan Tang
created: 2015-09-05T11:00:00Z
modified: 2015-09-18T09:45:18Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2009_
m user id: 20267
owner id: 20267
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: This is the cell display system of Yarrowia lipolytica, composed of LIP2 prepro, interest protein, and YLcwp3.LIP prepro is signal peptide used to secrete the interest protein out of the cell, and the YLcwp3 is the anchor domain binding the interest protein to the cell wall of yeast. We use this system to display silica-tag and test its binding characteristics. E.coli ribosomal protein L2 was found to bind tightly to silicon particles, which have surfaces that are oxidized to silica. This L2 silica-binding tag, called the ??????Si-tag,?????? can be used for one-step targeting of functional proteins on silica surfaces. The silica-binding domains of E. coli L2 was mapped to amino acids 1???60, 61-202 and 203???273, called Si-tag1, Si-tag2, Si-tag3. We respectively test the silica-binding characteristics of this three regions and their combinations.
mutable notes: In order to replace the domains more conveniently, we added BamHI, SalI, NdeI, NdeI between LIP2 prepro, E. coli ribosomal protein L2, GS linker and YLcwp3.
mutable provenance: E. coli ribosomal protein L2 was synthesized by IDT, LIP2 prepro and YLcwp3 were cloning from the plasmid JMP62, and we fused this three sequences.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: LIP2 prepro
sequence annotation: GS Linker
sequence annotation: Si-tag 2
sequence annotation: HIs6 tag
sequence annotation: SalI
sequence annotation: NdeI
sequence annotation: BamHI
sequence annotation: YLcwp3
sequence: https://synbiohub.org/public/igem/BBa_K1592008_sequence/1

LIP2 prepro
type: SequenceAnnotation
persistent identity: annotation2455910
display id: annotation2455910
version: 1
top level: Si-tag2
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1592008/annotation2455910/range2455910/1

https://synbiohub.org/public/igem/BBa_K1592008/annotation2455910/range2455910/1
type: Range
persistent identity: range2455910
display id: range2455910
version: 1
direction: direction/forward
top level: Si-tag2
start: 1
end: 99
orientation: inline

GS Linker
type: SequenceAnnotation
persistent identity: annotation2456104
display id: annotation2456104
version: 1
top level: Si-tag2
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1592008/annotation2456104/range2456104/1

https://synbiohub.org/public/igem/BBa_K1592008/annotation2456104/range2456104/1
type: Range
persistent identity: range2456104
display id: range2456104
version: 1
direction: direction/forward
top level: Si-tag2
start: 556
end: 600
orientation: inline

Si-tag 2
type: SequenceAnnotation
persistent identity: annotation2455957
display id: annotation2455957
version: 1
top level: Si-tag2
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1592008/annotation2455957/range2455957/1

https://synbiohub.org/public/igem/BBa_K1592008/annotation2455957/range2455957/1
type: Range
persistent identity: range2455957
display id: range2455957
version: 1
direction: direction/forward
top level: Si-tag2
start: 124
end: 549
orientation: inline

HIs6 tag
type: SequenceAnnotation
persistent identity: annotation2455913
display id: annotation2455913
version: 1
top level: Si-tag2
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1592008/annotation2455913/range2455913/1

https://synbiohub.org/public/igem/BBa_K1592008/annotation2455913/range2455913/1
type: Range
persistent identity: range2455913
display id: range2455913
version: 1
direction: direction/forward
top level: Si-tag2
start: 106
end: 123
orientation: inline

SalI
type: SequenceAnnotation
persistent identity: annotation2456026
display id: annotation2456026
version: 1
top level: Si-tag2
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1592008/annotation2456026/range2456026/1

https://synbiohub.org/public/igem/BBa_K1592008/annotation2456026/range2456026/1
type: Range
persistent identity: range2456026
display id: range2456026
version: 1
direction: direction/forward
top level: Si-tag2
start: 550
end: 555
orientation: inline

NdeI
type: SequenceAnnotation
persistent identity: annotation2456109
display id: annotation2456109
version: 1
top level: Si-tag2
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1592008/annotation2456109/range2456109/1

https://synbiohub.org/public/igem/BBa_K1592008/annotation2456109/range2456109/1
type: Range
persistent identity: range2456109
display id: range2456109
version: 1
direction: direction/forward
top level: Si-tag2
start: 601
end: 606
orientation: inline

BamHI
type: SequenceAnnotation
persistent identity: annotation2455912
display id: annotation2455912
version: 1
top level: Si-tag2
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1592008/annotation2455912/range2455912/1

https://synbiohub.org/public/igem/BBa_K1592008/annotation2455912/range2455912/1
type: Range
persistent identity: range2455912
display id: range2455912
version: 1
direction: direction/forward
top level: Si-tag2
start: 100
end: 105
orientation: inline

YLcwp3
type: SequenceAnnotation
persistent identity: annotation2456110
display id: annotation2456110
version: 1
top level: Si-tag2
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1592008/annotation2456110/range2456110/1

https://synbiohub.org/public/igem/BBa_K1592008/annotation2456110/range2456110/1
type: Range
persistent identity: range2456110
display id: range2456110
version: 1
direction: direction/forward
top level: Si-tag2
start: 607
end: 972
orientation: inline

BBa_K1674004
type: ComponentDefinition
persistent identity: BBa K1674004
display id: BBa_K1674004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: zwf - Glucose-6-Phosphate-Dehydrogenase from E.coli
creator: Roni Cohen
created: 2015-09-14T11:00:00Z
modified: 2015-09-18T12:17:43Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2092_
m user id: 25765
owner id: 25765
owning group id: 9
status: status/Planning
bookmark: true
mutable description: ZWF
mutable notes: ZWF
mutable provenance: ZWF
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1674004_sequence/1

SP-aprE
type: ComponentDefinition
persistent identity: BBa K1674000
display id: BBa_K1674000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Secretion signal peptide originated from the B.subtilis extracellular protease AprE
creator: Liron Abrahami
created: 2015-09-03T11:00:00Z
modified: 2015-09-17T08:12:50Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2092_
m user id: 26115
owner id: 26115
owning group id: 9
sample status: In stock
status: status/Available
status: status/Planning
bookmark: true
mutable description: 1
mutable notes: 1
mutable provenance: 1
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K1674000_sequence/1

RBS-Scaffo
type: ComponentDefinition
persistent identity: BBa K1602028
display id: BBa_K1602028
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Protein scaffold with N-terminal HisTag and RBS BBa_B0034
creator: Sebastian Barthel, Niklas Hummel, Jasmin Weber, Michelle Zoeller, Marcus Geissler, Stefan Bohn, Simon Linder
created: 2015-09-15T11:00:00Z
modified: 2015-09-16T06:25:50Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2019_
m user id: 22022
owner id: 22022
owning group id: 9
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1602027
component: BBa_B0034
sequence annotation: BBa_K1602027
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K1602028_sequence/1

BBa_K1602027
type: Component
persistent identity: component2462703
display id: component2462703
version: 1
top level: RBS-Scaffo
access: public
definition: His-Scaffo

BBa_B0034
type: Component
persistent identity: component2462702
display id: component2462702
version: 1
top level: RBS-Scaffo
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1602027
type: SequenceAnnotation
persistent identity: annotation2462703
display id: annotation2462703
version: 1
top level: RBS-Scaffo
location: https://synbiohub.org/public/igem/BBa_K1602028/annotation2462703/range2462703/1
component: BBa_K1602027

https://synbiohub.org/public/igem/BBa_K1602028/annotation2462703/range2462703/1
type: Range
persistent identity: range2462703
display id: range2462703
version: 1
direction: direction/reverse
top level: RBS-Scaffo
start: 19
end: 900
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2462702
display id: annotation2462702
version: 1
top level: RBS-Scaffo
location: https://synbiohub.org/public/igem/BBa_K1602028/annotation2462702/range2462702/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1602028/annotation2462702/range2462702/1
type: Range
persistent identity: range2462702
display id: range2462702
version: 1
direction: direction/reverse
top level: RBS-Scaffo
start: 1
end: 12
orientation: inline

BBa_K1666004
type: ComponentDefinition
persistent identity: BBa K1666004
display id: BBa_K1666004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LsrK of LuxS/AI-2 signaling pathway in Salmonalla
creator: Heming Wang
created: 2015-09-05T11:00:00Z
modified: 2015-09-18T09:14:01Z
discontinued: false
dominant: false
group u list: _2084_
m user id: 28478
owner id: 23948
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Quorum sensing is a process of bacterial cell-to-cell communication involving the production and detection of extracellular signaling molecules called autoinducers. And autoinducer-2 (AI-2) has been proposed to serve as a 'universal signal' for interspecies communication. In the LuxS/AI-2 signaling system of Salmonella Typhimurium, AI-2 response involves ATP binding cassette transporter encoded by genes named Lsr (LuxS regulated). And LsrK is the kinase which catalyzes the phosphorylation of autoinducer 2 (AI-2) to phospho-AI-2. In our project, we set this protein-coding part under a nisA promoter and try to integrate them in the genome of Lactobacillus or Lactococcus for the final purpose of constructing an integrated AI-2 response pathway of Salmonella in the engineered bacteria.
mutable notes: The lsrK gene was isolated from the genome of Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 using PCR. We added restriction enzyme sites when designing primers, and the assembly is done through the use of restriction sites (cutting and ligating).
mutable provenance: We extracted the genomic DNA of Salmonella enterica subsp. enterica serovar Typhimurium str. LT2, and used PCR to isolate the gene LsrK(Gene ID: 1255599).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: lsrK
sequence: https://synbiohub.org/public/igem/BBa_K1666004_sequence/1

lsrK
type: SequenceAnnotation
persistent identity: annotation2445114
display id: annotation2445114
version: 1
top level: BBa_K1666004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1666004/annotation2445114/range2445114/1

https://synbiohub.org/public/igem/BBa_K1666004/annotation2445114/range2445114/1
type: Range
persistent identity: range2445114
display id: range2445114
version: 1
direction: direction/forward
top level: BBa_K1666004
start: 1
end: 1593
orientation: inline

Art175
type: ComponentDefinition
persistent identity: BBa K1659000
display id: BBa_K1659000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Artilysin Art-175, an antibacterial fusion protein
creator: Wei Chung Kong
created: 2015-08-27T11:00:00Z
modified: 2016-02-01T12:51:21Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2077_
m user id: 4206
owner id: 24524
owning group id: 9
status: status/Planning
bookmark: false
mutable description: to be updated
mutable notes: to be updated
mutable provenance: to be updated
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Art-175
sequence annotation: Mistake
sequence annotation: Start
sequence annotation: Stop
sequence annotation: Hisx6
sequence: https://synbiohub.org/public/igem/BBa_K1659000_sequence/1

Art-175
type: SequenceAnnotation
persistent identity: annotation2440111
display id: annotation2440111
version: 1
top level: Art175
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1659000/annotation2440111/range2440111/1

https://synbiohub.org/public/igem/BBa_K1659000/annotation2440111/range2440111/1
type: Range
persistent identity: range2440111
display id: range2440111
version: 1
direction: direction/forward
top level: Art175
start: 7
end: 873
orientation: inline

Mistake
type: SequenceAnnotation
persistent identity: annotation2456677
display id: annotation2456677
version: 1
top level: Art175
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K1659000/annotation2456677/range2456677/1

https://synbiohub.org/public/igem/BBa_K1659000/annotation2456677/range2456677/1
type: Range
persistent identity: range2456677
display id: range2456677
version: 1
direction: direction/forward
top level: Art175
start: 4
end: 6
orientation: inline

Start
type: SequenceAnnotation
persistent identity: annotation2440110
display id: annotation2440110
version: 1
top level: Art175
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1659000/annotation2440110/range2440110/1

https://synbiohub.org/public/igem/BBa_K1659000/annotation2440110/range2440110/1
type: Range
persistent identity: range2440110
display id: range2440110
version: 1
direction: direction/forward
top level: Art175
start: 1
end: 3
orientation: inline

Stop
type: SequenceAnnotation
persistent identity: annotation2440113
display id: annotation2440113
version: 1
top level: Art175
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1659000/annotation2440113/range2440113/1

https://synbiohub.org/public/igem/BBa_K1659000/annotation2440113/range2440113/1
type: Range
persistent identity: range2440113
display id: range2440113
version: 1
direction: direction/forward
top level: Art175
start: 892
end: 897
orientation: inline

Hisx6
type: SequenceAnnotation
persistent identity: annotation2440112
display id: annotation2440112
version: 1
top level: Art175
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1659000/annotation2440112/range2440112/1

https://synbiohub.org/public/igem/BBa_K1659000/annotation2440112/range2440112/1
type: Range
persistent identity: range2440112
display id: range2440112
version: 1
direction: direction/forward
top level: Art175
start: 874
end: 891
orientation: inline

BBa_K1602044
type: ComponentDefinition
persistent identity: BBa K1602044
display id: BBa_K1602044
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RRL3H
creator: Christian Sator,Stefan Zens, Max Zander, Benedikt Spannenkrebs, Sebastian Jaeger
created: 2015-09-15T11:00:00Z
modified: 2015-09-25T05:18:30Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _2019_
m user id: 23028
owner id: 26220
owning group id: 9
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J23100
component: BBa_J01080
component: BBa_K1497008
sequence annotation: BBa_J01080
sequence annotation: BBa_K1497008
sequence annotation: BBa_J23100
sequence: https://synbiohub.org/public/igem/BBa_K1602044_sequence/1

BBa_J23100
type: Component
persistent identity: component2462882
display id: component2462882
version: 1
top level: BBa_K1602044
access: public
definition: https://synbiohub.org/public/igem/BBa_J23100/1

BBa_J01080
type: Component
persistent identity: component2462883
display id: component2462883
version: 1
top level: BBa_K1602044
access: public
definition: https://synbiohub.org/public/igem/BBa_J01080/1

BBa_K1497008
type: Component
persistent identity: component2462884
display id: component2462884
version: 1
top level: BBa_K1602044
access: public
definition: https://synbiohub.org/public/igem/BBa_K1497008/1

BBa_J01080
type: SequenceAnnotation
persistent identity: annotation2462883
display id: annotation2462883
version: 1
top level: BBa_K1602044
location: https://synbiohub.org/public/igem/BBa_K1602044/annotation2462883/range2462883/1
component: BBa_J01080

https://synbiohub.org/public/igem/BBa_K1602044/annotation2462883/range2462883/1
type: Range
persistent identity: range2462883
display id: range2462883
version: 1
direction: direction/reverse
top level: BBa_K1602044
start: 44
end: 83
orientation: inline

BBa_K1497008
type: SequenceAnnotation
persistent identity: annotation2462884
display id: annotation2462884
version: 1
top level: BBa_K1602044
location: https://synbiohub.org/public/igem/BBa_K1602044/annotation2462884/range2462884/1
component: BBa_K1497008

https://synbiohub.org/public/igem/BBa_K1602044/annotation2462884/range2462884/1
type: Range
persistent identity: range2462884
display id: range2462884
version: 1
direction: direction/reverse
top level: BBa_K1602044
start: 90
end: 245
orientation: inline

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2462882
display id: annotation2462882
version: 1
top level: BBa_K1602044
location: https://synbiohub.org/public/igem/BBa_K1602044/annotation2462882/range2462882/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K1602044/annotation2462882/range2462882/1
type: Range
persistent identity: range2462882
display id: range2462882
version: 1
direction: direction/reverse
top level: BBa_K1602044
start: 1
end: 35
orientation: inline

BBa_K1799004
type: ComponentDefinition
persistent identity: BBa K1799004
display id: BBa_K1799004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LsrC (wild type)
creator: Miranda Halle
created: 2015-08-06T11:00:00Z
modified: 2015-08-07T12:32:25Z
discontinued: false
dominant: false
group u list: _2224_
m user id: 19613
owner id: 19613
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Gene for the C subunit of the LsrACDB transporter, present in E. coli on the LsrACDBFG operon. The C subunit is a permease protein in the transporter, which actively brings autoinducer 2 (AI-2) into the cell.
mutable notes: The portion of the LsrACDBFG operon that codes for the AI-2 transporter consists of four open reading frames, which code for A, C, D, and B, respectively. Three of these ORFs overlap with one another: C overlaps with A, and its RBS is contained within the A subunit ORF, and D overlaps with C (although to a lesser extent), and its RBS is contained within the C subunit ORF. From a modularization standpoint, this creates an inefficiency because the wild type ORFs of the A and C subunits both contain functional RBSs, and in the case of the A subunit ORF, an ATG 4bp downstream. To reduce this inefficiency, we have created versions of each subunit ORF that are optimized for modularization. Further explanation is available in the descriptions of those parts.
mutable provenance: This part was synthesized from E. coli genomic data retrieved via EcoGene: http://www.ecogene.org/gene/EG13807
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1799004_sequence/1

mLacI
type: ComponentDefinition
persistent identity: BBa K1639010
display id: BBa_K1639010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: mammalian LacI with miR-373 binding site
creator: Nurgeldi Bazarov
created: 2015-09-14T11:00:00Z
modified: 2015-09-15T12:20:03Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2056_
m user id: 24789
owner id: 24789
owning group id: 9
status: status/Planning
bookmark: false
mutable description: In our project second step of cancer module is based on production of LacI so that it will suppress expression of DsRed. Binding site on 3' end of LacI makes it regulatable and limits it's expression only in cells with low miR-373 rate. In gastric epithelial cells level of this mirna is low but in gastric cancer cells level is very high.
mutable notes: We inserted two Mlu1 cut sites on each side of miR-373 binding so that other iGEM teams could easily replace it with any sequence.
mutable provenance: This protein is mammalian codon optimized version of LacI repressor originally derived from E.coli. It also possess nuclear localization signal (NLS) so that it can act on lac operators.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: miR-373 binding site
sequence annotation: Kozak
sequence annotation: Stop Codon
sequence annotation: mammalian LacI
sequence annotation: SV40 NLS
sequence annotation: ATG
sequence: https://synbiohub.org/public/igem/BBa_K1639010_sequence/1

miR-373 binding site
type: SequenceAnnotation
persistent identity: annotation2457338
display id: annotation2457338
version: 1
top level: mLacI
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1639010/annotation2457338/range2457338/1

https://synbiohub.org/public/igem/BBa_K1639010/annotation2457338/range2457338/1
type: Range
persistent identity: range2457338
display id: range2457338
version: 1
direction: direction/forward
top level: mLacI
start: 1141
end: 1163
orientation: inline

Kozak
type: SequenceAnnotation
persistent identity: annotation2457312
display id: annotation2457312
version: 1
top level: mLacI
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1639010/annotation2457312/range2457312/1

https://synbiohub.org/public/igem/BBa_K1639010/annotation2457312/range2457312/1
type: Range
persistent identity: range2457312
display id: range2457312
version: 1
direction: direction/forward
top level: mLacI
start: 1
end: 6
orientation: inline

Stop Codon
type: SequenceAnnotation
persistent identity: annotation2457337
display id: annotation2457337
version: 1
top level: mLacI
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1639010/annotation2457337/range2457337/1

https://synbiohub.org/public/igem/BBa_K1639010/annotation2457337/range2457337/1
type: Range
persistent identity: range2457337
display id: range2457337
version: 1
direction: direction/forward
top level: mLacI
start: 1123
end: 1134
orientation: inline

mammalian LacI
type: SequenceAnnotation
persistent identity: annotation2457315
display id: annotation2457315
version: 1
top level: mLacI
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1639010/annotation2457315/range2457315/1

https://synbiohub.org/public/igem/BBa_K1639010/annotation2457315/range2457315/1
type: Range
persistent identity: range2457315
display id: range2457315
version: 1
direction: direction/forward
top level: mLacI
start: 7
end: 1125
orientation: inline

SV40 NLS
type: SequenceAnnotation
persistent identity: annotation2457336
display id: annotation2457336
version: 1
top level: mLacI
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1639010/annotation2457336/range2457336/1

https://synbiohub.org/public/igem/BBa_K1639010/annotation2457336/range2457336/1
type: Range
persistent identity: range2457336
display id: range2457336
version: 1
direction: direction/forward
top level: mLacI
start: 1102
end: 1122
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2457339
display id: annotation2457339
version: 1
top level: mLacI
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1639010/annotation2457339/range2457339/1

https://synbiohub.org/public/igem/BBa_K1639010/annotation2457339/range2457339/1
type: Range
persistent identity: range2457339
display id: range2457339
version: 1
direction: direction/forward
top level: mLacI
start: 7
end: 9
orientation: inline

BBa_K1763438
type: ComponentDefinition
persistent identity: BBa K1763438
display id: BBa_K1763438
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: MaSp1/2[2:1]-12(1C3)
creator: Vinson Lam
created: 2015-09-14T11:00:00Z
modified: 2015-09-16T11:57:37Z
discontinued: false
dominant: false
group u list: _2185_
m user id: 27136
owner id: 27136
owning group id: 9
status: status/Planning
bookmark: false
mutable description: MaSp1 and MaSp2 12-mer block co-polymer in pSB1C3, no regulatory elements. Ratio of 2 MaSp1 to 1 MaSp2.
mutable notes: no
mutable provenance: N. clavipes
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: 8-his tag
sequence annotation: M2-AB
sequence annotation: M1-CA
sequence annotation: M2-CA
sequence annotation: M1-AB
sequence annotation: M1-BC
sequence annotation: M2-BC
sequence: https://synbiohub.org/public/igem/BBa_K1763438_sequence/1

8-his tag
type: SequenceAnnotation
persistent identity: annotation2464087
display id: annotation2464087
version: 1
top level: BBa_K1763438
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1763438/annotation2464087/range2464087/1

https://synbiohub.org/public/igem/BBa_K1763438/annotation2464087/range2464087/1
type: Range
persistent identity: range2464087
display id: range2464087
version: 1
direction: direction/forward
top level: BBa_K1763438
start: 4
end: 27
orientation: inline

M2-AB
type: SequenceAnnotation
persistent identity: annotation2464097
display id: annotation2464097
version: 1
top level: BBa_K1763438
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763438/annotation2464097/range2464097/1

https://synbiohub.org/public/igem/BBa_K1763438/annotation2464097/range2464097/1
type: Range
persistent identity: range2464097
display id: range2464097
version: 1
direction: direction/forward
top level: BBa_K1763438
start: 954
end: 1055
orientation: inline

M1-CA
type: SequenceAnnotation
persistent identity: annotation2464093
display id: annotation2464093
version: 1
top level: BBa_K1763438
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763438/annotation2464093/range2464093/1

https://synbiohub.org/public/igem/BBa_K1763438/annotation2464093/range2464093/1
type: Range
persistent identity: range2464093
display id: range2464093
version: 1
direction: direction/forward
top level: BBa_K1763438
start: 546
end: 647
orientation: inline

M2-CA
type: SequenceAnnotation
persistent identity: annotation2464096
display id: annotation2464096
version: 1
top level: BBa_K1763438
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763438/annotation2464096/range2464096/1

https://synbiohub.org/public/igem/BBa_K1763438/annotation2464096/range2464096/1
type: Range
persistent identity: range2464096
display id: range2464096
version: 1
direction: direction/forward
top level: BBa_K1763438
start: 852
end: 953
orientation: inline

M1-AB
type: SequenceAnnotation
persistent identity: annotation2464088
display id: annotation2464088
version: 1
top level: BBa_K1763438
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763438/annotation2464088/range2464088/1

https://synbiohub.org/public/igem/BBa_K1763438/annotation2464088/range2464088/1
type: Range
persistent identity: range2464088
display id: range2464088
version: 1
direction: direction/forward
top level: BBa_K1763438
start: 36
end: 137
orientation: inline

M1-CA
type: SequenceAnnotation
persistent identity: annotation2464090
display id: annotation2464090
version: 1
top level: BBa_K1763438
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763438/annotation2464090/range2464090/1

https://synbiohub.org/public/igem/BBa_K1763438/annotation2464090/range2464090/1
type: Range
persistent identity: range2464090
display id: range2464090
version: 1
direction: direction/forward
top level: BBa_K1763438
start: 240
end: 341
orientation: inline

M1-BC
type: SequenceAnnotation
persistent identity: annotation2464095
display id: annotation2464095
version: 1
top level: BBa_K1763438
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763438/annotation2464095/range2464095/1

https://synbiohub.org/public/igem/BBa_K1763438/annotation2464095/range2464095/1
type: Range
persistent identity: range2464095
display id: range2464095
version: 1
direction: direction/forward
top level: BBa_K1763438
start: 750
end: 851
orientation: inline

M2-CA
type: SequenceAnnotation
persistent identity: annotation2464085
display id: annotation2464085
version: 1
top level: BBa_K1763438
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763438/annotation2464085/range2464085/1

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type: Range
persistent identity: range2464085
display id: range2464085
version: 1
direction: direction/forward
top level: BBa_K1763438
start: 1158
end: 1259
orientation: inline

M1-BC
type: SequenceAnnotation
persistent identity: annotation2464089
display id: annotation2464089
version: 1
top level: BBa_K1763438
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763438/annotation2464089/range2464089/1

https://synbiohub.org/public/igem/BBa_K1763438/annotation2464089/range2464089/1
type: Range
persistent identity: range2464089
display id: range2464089
version: 1
direction: direction/forward
top level: BBa_K1763438
start: 138
end: 239
orientation: inline

M1-BC
type: SequenceAnnotation
persistent identity: annotation2464092
display id: annotation2464092
version: 1
top level: BBa_K1763438
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763438/annotation2464092/range2464092/1

https://synbiohub.org/public/igem/BBa_K1763438/annotation2464092/range2464092/1
type: Range
persistent identity: range2464092
display id: range2464092
version: 1
direction: direction/forward
top level: BBa_K1763438
start: 444
end: 545
orientation: inline

M1-AB
type: SequenceAnnotation
persistent identity: annotation2464091
display id: annotation2464091
version: 1
top level: BBa_K1763438
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763438/annotation2464091/range2464091/1

https://synbiohub.org/public/igem/BBa_K1763438/annotation2464091/range2464091/1
type: Range
persistent identity: range2464091
display id: range2464091
version: 1
direction: direction/forward
top level: BBa_K1763438
start: 342
end: 443
orientation: inline

M2-BC
type: SequenceAnnotation
persistent identity: annotation2464086
display id: annotation2464086
version: 1
top level: BBa_K1763438
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763438/annotation2464086/range2464086/1

https://synbiohub.org/public/igem/BBa_K1763438/annotation2464086/range2464086/1
type: Range
persistent identity: range2464086
display id: range2464086
version: 1
direction: direction/forward
top level: BBa_K1763438
start: 1056
end: 1157
orientation: inline

M1-AB
type: SequenceAnnotation
persistent identity: annotation2464094
display id: annotation2464094
version: 1
top level: BBa_K1763438
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763438/annotation2464094/range2464094/1

https://synbiohub.org/public/igem/BBa_K1763438/annotation2464094/range2464094/1
type: Range
persistent identity: range2464094
display id: range2464094
version: 1
direction: direction/forward
top level: BBa_K1763438
start: 648
end: 749
orientation: inline

BBa_K1602043
type: ComponentDefinition
persistent identity: BBa K1602043
display id: BBa_K1602043
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: araCpBad-hokD
creator: Christian Sator,Stefan Zens, Max Zander, Benedikt Spannenkrebs, Sebastian Jaeger
created: 2015-09-15T11:00:00Z
modified: 2015-09-25T05:19:21Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2019_
m user id: 23028
owner id: 26220
owning group id: 9
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K1497008
component: BBa_K808000
component: BBa_B0034
sequence annotation: BBa_K808000
sequence annotation: BBa_K1497008
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K1602043_sequence/1

BBa_K1497008
type: Component
persistent identity: component2462892
display id: component2462892
version: 1
top level: BBa_K1602043
access: public
definition: https://synbiohub.org/public/igem/BBa_K1497008/1

BBa_K808000
type: Component
persistent identity: component2462889
display id: component2462889
version: 1
top level: BBa_K1602043
access: public
definition: https://synbiohub.org/public/igem/BBa_K808000/1

BBa_B0034
type: Component
persistent identity: component2462891
display id: component2462891
version: 1
top level: BBa_K1602043
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K808000
type: SequenceAnnotation
persistent identity: annotation2462889
display id: annotation2462889
version: 1
top level: BBa_K1602043
location: https://synbiohub.org/public/igem/BBa_K1602043/annotation2462889/range2462889/1
component: BBa_K808000

https://synbiohub.org/public/igem/BBa_K1602043/annotation2462889/range2462889/1
type: Range
persistent identity: range2462889
display id: range2462889
version: 1
direction: direction/reverse
top level: BBa_K1602043
start: 1
end: 1209
orientation: inline

BBa_K1497008
type: SequenceAnnotation
persistent identity: annotation2462892
display id: annotation2462892
version: 1
top level: BBa_K1602043
location: https://synbiohub.org/public/igem/BBa_K1602043/annotation2462892/range2462892/1
component: BBa_K1497008

https://synbiohub.org/public/igem/BBa_K1602043/annotation2462892/range2462892/1
type: Range
persistent identity: range2462892
display id: range2462892
version: 1
direction: direction/reverse
top level: BBa_K1602043
start: 1236
end: 1391
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2462891
display id: annotation2462891
version: 1
top level: BBa_K1602043
location: https://synbiohub.org/public/igem/BBa_K1602043/annotation2462891/range2462891/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1602043/annotation2462891/range2462891/1
type: Range
persistent identity: range2462891
display id: range2462891
version: 1
direction: direction/reverse
top level: BBa_K1602043
start: 1218
end: 1229
orientation: inline

BBa_K1763439
type: ComponentDefinition
persistent identity: BBa K1763439
display id: BBa_K1763439
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: MaSp1/2[2:1]-12(T7)
creator: Vinson Lam
created: 2015-09-14T11:00:00Z
modified: 2015-09-15T09:39:57Z
discontinued: false
dominant: false
group u list: _2185_
m user id: 27136
owner id: 27136
owning group id: 9
status: status/Planning
bookmark: false
mutable description: MaSp1 and MaSp2 12-mer block co-polymer under control of T7 promoter and RBS. Ratio of 2 MaSp1 to 1 MaSp2.
mutable notes: no
mutable provenance: N. clavipes
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K525998
component: BBa_K1763438
sequence annotation: BBa_K525998
sequence annotation: BBa_K1763438
sequence: https://synbiohub.org/public/igem/BBa_K1763439_sequence/1

BBa_K525998
type: Component
persistent identity: component2456897
display id: component2456897
version: 1
top level: BBa_K1763439
access: public
definition: https://synbiohub.org/public/igem/BBa_K525998/1

BBa_K1763438
type: Component
persistent identity: component2456898
display id: component2456898
version: 1
top level: BBa_K1763439
access: public
definition: BBa_K1763438

BBa_K525998
type: SequenceAnnotation
persistent identity: annotation2456897
display id: annotation2456897
version: 1
top level: BBa_K1763439
location: https://synbiohub.org/public/igem/BBa_K1763439/annotation2456897/range2456897/1
component: BBa_K525998

https://synbiohub.org/public/igem/BBa_K1763439/annotation2456897/range2456897/1
type: Range
persistent identity: range2456897
display id: range2456897
version: 1
direction: direction/reverse
top level: BBa_K1763439
start: 1
end: 32
orientation: inline

BBa_K1763438
type: SequenceAnnotation
persistent identity: annotation2456898
display id: annotation2456898
version: 1
top level: BBa_K1763439
location: https://synbiohub.org/public/igem/BBa_K1763439/annotation2456898/range2456898/1
component: BBa_K1763438

https://synbiohub.org/public/igem/BBa_K1763439/annotation2456898/range2456898/1
type: Range
persistent identity: range2456898
display id: range2456898
version: 1
direction: direction/reverse
top level: BBa_K1763439
start: 39
end: 1313
orientation: inline

DsbDspBx
type: ComponentDefinition
persistent identity: BBa K1659211
display id: BBa_K1659211
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Dispersin B fused with DsbA, with mutation at nucleotide 636 (T to C)
creator: Wei Chung Kong
created: 2015-08-27T11:00:00Z
modified: 2015-09-14T04:16:28Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2077_
m user id: 24524
owner id: 24524
owning group id: 9
status: status/Planning
bookmark: false
mutable description: to be updated
mutable notes: to be updated
mutable provenance: to be updated
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: Hisx6
sequence annotation: stop
sequence annotation: start
sequence annotation: DsbA 2-19 signal sequence
sequence annotation: DspBx
sequence: https://synbiohub.org/public/igem/BBa_K1659211_sequence/1

Hisx6
type: SequenceAnnotation
persistent identity: annotation2452808
display id: annotation2452808
version: 1
top level: DsbDspBx
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1659211/annotation2452808/range2452808/1

https://synbiohub.org/public/igem/BBa_K1659211/annotation2452808/range2452808/1
type: Range
persistent identity: range2452808
display id: range2452808
version: 1
direction: direction/forward
top level: DsbDspBx
start: 1141
end: 1158
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2452809
display id: annotation2452809
version: 1
top level: DsbDspBx
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1659211/annotation2452809/range2452809/1

https://synbiohub.org/public/igem/BBa_K1659211/annotation2452809/range2452809/1
type: Range
persistent identity: range2452809
display id: range2452809
version: 1
direction: direction/forward
top level: DsbDspBx
start: 1159
end: 1164
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2452805
display id: annotation2452805
version: 1
top level: DsbDspBx
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1659211/annotation2452805/range2452805/1

https://synbiohub.org/public/igem/BBa_K1659211/annotation2452805/range2452805/1
type: Range
persistent identity: range2452805
display id: range2452805
version: 1
direction: direction/forward
top level: DsbDspBx
start: 1
end: 3
orientation: inline

DsbA 2-19 signal sequence
type: SequenceAnnotation
persistent identity: annotation2452806
display id: annotation2452806
version: 1
top level: DsbDspBx
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1659211/annotation2452806/range2452806/1

https://synbiohub.org/public/igem/BBa_K1659211/annotation2452806/range2452806/1
type: Range
persistent identity: range2452806
display id: range2452806
version: 1
direction: direction/forward
top level: DsbDspBx
start: 4
end: 57
orientation: inline

DspBx
type: SequenceAnnotation
persistent identity: annotation2452807
display id: annotation2452807
version: 1
top level: DsbDspBx
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1659211/annotation2452807/range2452807/1

https://synbiohub.org/public/igem/BBa_K1659211/annotation2452807/range2452807/1
type: Range
persistent identity: range2452807
display id: range2452807
version: 1
direction: direction/forward
top level: DsbDspBx
start: 58
end: 1140
orientation: inline

BBa_K1763443
type: ComponentDefinition
persistent identity: BBa K1763443
display id: BBa_K1763443
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: MaSp1/2[1:2]-12(T7)
creator: Vinson Lam
created: 2015-09-14T11:00:00Z
modified: 2015-09-15T09:53:21Z
discontinued: false
dominant: false
group u list: _2185_
m user id: 27136
owner id: 27136
owning group id: 9
status: status/Planning
bookmark: false
mutable description: MaSp1 and MaSp 12-mer block co-polymer under control of T7 promoter and RBS
mutable notes: no.
mutable provenance: N. clavipes
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K1763442
component: BBa_K525998
sequence annotation: BBa_K1763442
sequence annotation: BBa_K525998
sequence: https://synbiohub.org/public/igem/BBa_K1763443_sequence/1

BBa_K1763442
type: Component
persistent identity: component2456986
display id: component2456986
version: 1
top level: BBa_K1763443
access: public
definition: BBa_K1763442

BBa_K525998
type: Component
persistent identity: component2456985
display id: component2456985
version: 1
top level: BBa_K1763443
access: public
definition: https://synbiohub.org/public/igem/BBa_K525998/1

BBa_K1763442
type: SequenceAnnotation
persistent identity: annotation2456986
display id: annotation2456986
version: 1
top level: BBa_K1763443
location: https://synbiohub.org/public/igem/BBa_K1763443/annotation2456986/range2456986/1
component: BBa_K1763442

https://synbiohub.org/public/igem/BBa_K1763443/annotation2456986/range2456986/1
type: Range
persistent identity: range2456986
display id: range2456986
version: 1
direction: direction/reverse
top level: BBa_K1763443
start: 39
end: 1313
orientation: inline

BBa_K525998
type: SequenceAnnotation
persistent identity: annotation2456985
display id: annotation2456985
version: 1
top level: BBa_K1763443
location: https://synbiohub.org/public/igem/BBa_K1763443/annotation2456985/range2456985/1
component: BBa_K525998

https://synbiohub.org/public/igem/BBa_K1763443/annotation2456985/range2456985/1
type: Range
persistent identity: range2456985
display id: range2456985
version: 1
direction: direction/reverse
top level: BBa_K1763443
start: 1
end: 32
orientation: inline

BBa_K1699002
type: ComponentDefinition
persistent identity: BBa K1699002
display id: BBa_K1699002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: gRNA for SaCas9 targeting human ubiquitin B gene
creator: Emil Ruvinov
created: 2015-08-08T11:00:00Z
modified: 2015-09-13T06:26:57Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2117_
m user id: 24826
owner id: 24826
owning group id: 9
status: status/Planning
bookmark: false
mutable description: gRNA for SaCas9 targeting ubiquitin B
mutable notes: design
mutable provenance: source
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: gRNA-UBB-Exon 2
sequence annotation: HDV ribozyme
sequence annotation: Hammerhead ribozyme
sequence annotation: gRNA scaffold for SaCas9
sequence: https://synbiohub.org/public/igem/BBa_K1699002_sequence/1

gRNA-UBB-Exon 2
type: SequenceAnnotation
persistent identity: annotation2434596
display id: annotation2434596
version: 1
top level: BBa_K1699002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1699002/annotation2434596/range2434596/1

https://synbiohub.org/public/igem/BBa_K1699002/annotation2434596/range2434596/1
type: Range
persistent identity: range2434596
display id: range2434596
version: 1
direction: direction/forward
top level: BBa_K1699002
start: 44
end: 65
orientation: inline

HDV ribozyme
type: SequenceAnnotation
persistent identity: annotation2434598
display id: annotation2434598
version: 1
top level: BBa_K1699002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1699002/annotation2434598/range2434598/1

https://synbiohub.org/public/igem/BBa_K1699002/annotation2434598/range2434598/1
type: Range
persistent identity: range2434598
display id: range2434598
version: 1
direction: direction/forward
top level: BBa_K1699002
start: 147
end: 212
orientation: inline

Hammerhead ribozyme
type: SequenceAnnotation
persistent identity: annotation2434595
display id: annotation2434595
version: 1
top level: BBa_K1699002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1699002/annotation2434595/range2434595/1

https://synbiohub.org/public/igem/BBa_K1699002/annotation2434595/range2434595/1
type: Range
persistent identity: range2434595
display id: range2434595
version: 1
direction: direction/forward
top level: BBa_K1699002
start: 1
end: 43
orientation: inline

gRNA scaffold for SaCas9
type: SequenceAnnotation
persistent identity: annotation2434597
display id: annotation2434597
version: 1
top level: BBa_K1699002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1699002/annotation2434597/range2434597/1

https://synbiohub.org/public/igem/BBa_K1699002/annotation2434597/range2434597/1
type: Range
persistent identity: range2434597
display id: range2434597
version: 1
direction: direction/forward
top level: BBa_K1699002
start: 66
end: 146
orientation: inline

BBa_K1758203
type: ComponentDefinition
persistent identity: BBa K1758203
display id: BBa_K1758203
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: His-tagged arsenic repressor fused to superfolder GFP under the control of T7 promoter
creator: Team Bielefeld-CeBiTec 2015
created: 2015-08-28T11:00:00Z
modified: 2015-09-18T05:53:38Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2180_
m user id: 26913
owner id: 26913
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Uses T7 promotor and a strong rbs (BBa_K525998), the arsenic repressor linked with superfolding GFP (sfGFP) with a His-Tag. By linking sfGFP we want to detect bound protein via fluorescence.
mutable notes: We clone arsR in T7-RBS-sfGFP and added a His-Tag via PCR and Gibson Assembly.
mutable provenance: This is a composite part which enables a high protein expression and an easy purification of arsR fused to superfolding GFP (sfGFP). The green fluorescence of the culture is a sign for protein expression.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: T7 Promoter
sequence annotation: BBa_B0010
sequence annotation: RBS
sequence annotation: BBa_B0012
sequence annotation: His-Tag
sequence annotation: BBa_K525998
sequence annotation: arsR
sequence annotation: sfGFP
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K1758203_sequence/1

T7 Promoter
type: SequenceAnnotation
persistent identity: annotation2440039
display id: annotation2440039
version: 1
top level: BBa_K1758203
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1758203/annotation2440039/range2440039/1

https://synbiohub.org/public/igem/BBa_K1758203/annotation2440039/range2440039/1
type: Range
persistent identity: range2440039
display id: range2440039
version: 1
direction: direction/forward
top level: BBa_K1758203
start: 1
end: 23
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2440043
display id: annotation2440043
version: 1
top level: BBa_K1758203
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1758203/annotation2440043/range2440043/1

https://synbiohub.org/public/igem/BBa_K1758203/annotation2440043/range2440043/1
type: Range
persistent identity: range2440043
display id: range2440043
version: 1
direction: direction/forward
top level: BBa_K1758203
start: 1155
end: 1234
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2440038
display id: annotation2440038
version: 1
top level: BBa_K1758203
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1758203/annotation2440038/range2440038/1

https://synbiohub.org/public/igem/BBa_K1758203/annotation2440038/range2440038/1
type: Range
persistent identity: range2440038
display id: range2440038
version: 1
direction: direction/forward
top level: BBa_K1758203
start: 24
end: 27
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2440044
display id: annotation2440044
version: 1
top level: BBa_K1758203
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1758203/annotation2440044/range2440044/1

https://synbiohub.org/public/igem/BBa_K1758203/annotation2440044/range2440044/1
type: Range
persistent identity: range2440044
display id: range2440044
version: 1
direction: direction/forward
top level: BBa_K1758203
start: 1243
end: 1283
orientation: inline

His-Tag
type: SequenceAnnotation
persistent identity: annotation2440040
display id: annotation2440040
version: 1
top level: BBa_K1758203
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1758203/annotation2440040/range2440040/1

https://synbiohub.org/public/igem/BBa_K1758203/annotation2440040/range2440040/1
type: Range
persistent identity: range2440040
display id: range2440040
version: 1
direction: direction/forward
top level: BBa_K1758203
start: 1123
end: 1146
orientation: inline

BBa_K525998
type: SequenceAnnotation
persistent identity: annotation2440037
display id: annotation2440037
version: 1
top level: BBa_K1758203
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1758203/annotation2440037/range2440037/1

https://synbiohub.org/public/igem/BBa_K1758203/annotation2440037/range2440037/1
type: Range
persistent identity: range2440037
display id: range2440037
version: 1
direction: direction/forward
top level: BBa_K1758203
start: 1
end: 32
orientation: inline

arsR
type: SequenceAnnotation
persistent identity: annotation2440041
display id: annotation2440041
version: 1
top level: BBa_K1758203
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1758203/annotation2440041/range2440041/1

https://synbiohub.org/public/igem/BBa_K1758203/annotation2440041/range2440041/1
type: Range
persistent identity: range2440041
display id: range2440041
version: 1
direction: direction/forward
top level: BBa_K1758203
start: 50
end: 402
orientation: inline

sfGFP
type: SequenceAnnotation
persistent identity: annotation2440042
display id: annotation2440042
version: 1
top level: BBa_K1758203
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1758203/annotation2440042/range2440042/1

https://synbiohub.org/public/igem/BBa_K1758203/annotation2440042/range2440042/1
type: Range
persistent identity: range2440042
display id: range2440042
version: 1
direction: direction/forward
top level: BBa_K1758203
start: 409
end: 1122
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2440036
display id: annotation2440036
version: 1
top level: BBa_K1758203
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1758203/annotation2440036/range2440036/1

https://synbiohub.org/public/igem/BBa_K1758203/annotation2440036/range2440036/1
type: Range
persistent identity: range2440036
display id: range2440036
version: 1
direction: direction/forward
top level: BBa_K1758203
start: 21
end: 32
orientation: inline

BBa_K1789010
type: ComponentDefinition
persistent identity: BBa K1789010
display id: BBa_K1789010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TALE3
creator: Xinyuan Qiu
created: 2015-09-13T11:00:00Z
modified: 2015-09-14T07:28:23Z
discontinued: false
dominant: false
group u list: _2214_
m user id: 21134
owner id: 21134
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This is a engineered TAL effector that can bind with the DNA sequence 5'-CTTTCCACAAATAG-3'
mutable notes: The part sequence should be able to correctly and efficiently bind with corresponding DNA, so we used bioinformatics tools to help us designing this sequence.
mutable provenance: This part is built and standardized by ourselves using GATEWAY technique.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: DNA Binding Domain
sequence annotation: TALE3
sequence: https://synbiohub.org/public/igem/BBa_K1789010_sequence/1

DNA Binding Domain
type: SequenceAnnotation
persistent identity: annotation2454136
display id: annotation2454136
version: 1
top level: BBa_K1789010
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1789010/annotation2454136/range2454136/1

https://synbiohub.org/public/igem/BBa_K1789010/annotation2454136/range2454136/1
type: Range
persistent identity: range2454136
display id: range2454136
version: 1
direction: direction/forward
top level: BBa_K1789010
start: 508
end: 1872
orientation: inline

TALE3
type: SequenceAnnotation
persistent identity: annotation2454135
display id: annotation2454135
version: 1
top level: BBa_K1789010
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1789010/annotation2454135/range2454135/1

https://synbiohub.org/public/igem/BBa_K1789010/annotation2454135/range2454135/1
type: Range
persistent identity: range2454135
display id: range2454135
version: 1
direction: direction/forward
top level: BBa_K1789010
start: 1
end: 2088
orientation: inline

BBa_K1789011
type: ComponentDefinition
persistent identity: BBa K1789011
display id: BBa_K1789011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: IAAH+Ter
creator: Jiaqi Sun
created: 2015-09-13T11:00:00Z
modified: 2015-09-18T10:46:33Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2214_
m user id: 21134
owner id: 26298
owning group id: 9
status: status/Planning
bookmark: false
mutable description: It is the combination of IAAH and termination.
mutable notes: We combine IAAH and the termination to improve the efficiency of the building.
mutable provenance: It is constructed by ourselves.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_K1789001
component: BBa_B0015
sequence annotation: BBa_B0015
sequence annotation: BBa_K1789001
sequence: https://synbiohub.org/public/igem/BBa_K1789011_sequence/1

BBa_K1789001
type: Component
persistent identity: component2454246
display id: component2454246
version: 1
top level: BBa_K1789011
access: public
definition: BBa_K1789001

BBa_B0015
type: Component
persistent identity: component2454253
display id: component2454253
version: 1
top level: BBa_K1789011
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2454253
display id: annotation2454253
version: 1
top level: BBa_K1789011
location: https://synbiohub.org/public/igem/BBa_K1789011/annotation2454253/range2454253/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1789011/annotation2454253/range2454253/1
type: Range
persistent identity: range2454253
display id: range2454253
version: 1
direction: direction/reverse
top level: BBa_K1789011
start: 1344
end: 1472
orientation: inline

BBa_K1789001
type: SequenceAnnotation
persistent identity: annotation2454246
display id: annotation2454246
version: 1
top level: BBa_K1789011
location: https://synbiohub.org/public/igem/BBa_K1789011/annotation2454246/range2454246/1
component: BBa_K1789001

https://synbiohub.org/public/igem/BBa_K1789011/annotation2454246/range2454246/1
type: Range
persistent identity: range2454246
display id: range2454246
version: 1
direction: direction/reverse
top level: BBa_K1789011
start: 1
end: 1335
orientation: inline

BBa_K1614020
type: ComponentDefinition
persistent identity: BBa K1614020
display id: BBa_K1614020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ATP Aptamer JAWS2 Spinach 2.1
creator: Daniel Heid
created: 2015-09-17T11:00:00Z
modified: 2015-09-18T09:19:49Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2031_
m user id: 16422
owner id: 24635
owning group id: 9
status: status/Planning
bookmark: false
mutable description: ATP Spinach
mutable provenance: Synthetic
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence: https://synbiohub.org/public/igem/BBa_K1614020_sequence/1

BBa_K1614016
type: ComponentDefinition
persistent identity: BBa K1614016
display id: BBa_K1614016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Hammerhead Ribozyme and Malchite Green Aptamer in BBF RFC 110 transcription cassette
creator: Frieda Anna Sorgenfrei
created: 2015-09-15T11:00:00Z
modified: 2015-09-16T05:09:22Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2031_
m user id: 24664
owner id: 24664
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Hammerhead Ribozyme (HHR) and Malchite Green (MG) Aptamer in BBF RFC 110 transcription cassette for live tracking of in vitro transcription.
mutable notes: Cloned without restriction enzymes to avoid issues occurring from conserved cut sites. For more details see BBF RFC 110.
mutable provenance: Synthetic, Malchit Green aptamer (Grate, D., and Wilson, C. (1999). Laser-mediated, site-specific inactivation of RNA transcripts. Proceedings of the National Academy of Sciences of the United States of America 96, 6131-6136.) HHR (Prody, G.A., Bakos, J.T., Buzayan, J.M., Schneider, I.R., and Bruening, G. (1986). Autolytic processing of dimeric plant virus satellite RNA. Science 231, 1577-1580)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence annotation: HDV
sequence annotation: G required for transcription
sequence annotation: T7 promotor
sequence annotation: HHR
sequence annotation: Malachite Green Aptamer
sequence: https://synbiohub.org/public/igem/BBa_K1614016_sequence/1

HDV
type: SequenceAnnotation
persistent identity: annotation2459506
display id: annotation2459506
version: 1
top level: BBa_K1614016
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1614016/annotation2459506/range2459506/1

https://synbiohub.org/public/igem/BBa_K1614016/annotation2459506/range2459506/1
type: Range
persistent identity: range2459506
display id: range2459506
version: 1
direction: direction/forward
top level: BBa_K1614016
start: 123
end: 206
orientation: inline

G required for transcription
type: SequenceAnnotation
persistent identity: annotation2459467
display id: annotation2459467
version: 1
top level: BBa_K1614016
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1614016/annotation2459467/range2459467/1

https://synbiohub.org/public/igem/BBa_K1614016/annotation2459467/range2459467/1
type: Range
persistent identity: range2459467
display id: range2459467
version: 1
direction: direction/forward
top level: BBa_K1614016
start: 30
end: 30
orientation: inline

T7 promotor
type: SequenceAnnotation
persistent identity: annotation2459466
display id: annotation2459466
version: 1
top level: BBa_K1614016
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1614016/annotation2459466/range2459466/1

https://synbiohub.org/public/igem/BBa_K1614016/annotation2459466/range2459466/1
type: Range
persistent identity: range2459466
display id: range2459466
version: 1
direction: direction/forward
top level: BBa_K1614016
start: 13
end: 29
orientation: inline

HHR
type: SequenceAnnotation
persistent identity: annotation2459489
display id: annotation2459489
version: 1
top level: BBa_K1614016
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1614016/annotation2459489/range2459489/1

https://synbiohub.org/public/igem/BBa_K1614016/annotation2459489/range2459489/1
type: Range
persistent identity: range2459489
display id: range2459489
version: 1
direction: direction/forward
top level: BBa_K1614016
start: 31
end: 84
orientation: inline

Malachite Green Aptamer
type: SequenceAnnotation
persistent identity: annotation2459490
display id: annotation2459490
version: 1
top level: BBa_K1614016
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1614016/annotation2459490/range2459490/1

https://synbiohub.org/public/igem/BBa_K1614016/annotation2459490/range2459490/1
type: Range
persistent identity: range2459490
display id: range2459490
version: 1
direction: direction/forward
top level: BBa_K1614016
start: 85
end: 122
orientation: inline

BBa_K1689017
type: ComponentDefinition
persistent identity: BBa K1689017
display id: BBa_K1689017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: F3-dCas9
creator: Wei weijia
created: 2015-09-15T11:00:00Z
modified: 2015-09-27T01:38:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2107_
m user id: 26531
owner id: 26531
owning group id: 9
status: status/Planning
bookmark: false
mutable description: pSB1C3-6H-F3-dCas9, fusion protein of F3 segment of DHFR fused with dCas9, the His tag facilitates protein purification
mutable notes: The coding sequence of this fusion protein is too long, you'd better amplify is separately.
mutable provenance: E.coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: F3
sequence annotation: His tag
sequence annotation: dCas9
sequence annotation: F3-dCas9
sequence annotation: random spacer
sequence annotation: start
sequence annotation: stop
sequence annotation: GGGGS*2 Linker
sequence: https://synbiohub.org/public/igem/BBa_K1689017_sequence/1

F3
type: SequenceAnnotation
persistent identity: annotation2469978
display id: annotation2469978
version: 1
top level: BBa_K1689017
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1689017/annotation2469978/range2469978/1

https://synbiohub.org/public/igem/BBa_K1689017/annotation2469978/range2469978/1
type: Range
persistent identity: range2469978
display id: range2469978
version: 1
direction: direction/forward
top level: BBa_K1689017
start: 55
end: 267
orientation: inline

His tag
type: SequenceAnnotation
persistent identity: annotation2469977
display id: annotation2469977
version: 1
top level: BBa_K1689017
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1689017/annotation2469977/range2469977/1

https://synbiohub.org/public/igem/BBa_K1689017/annotation2469977/range2469977/1
type: Range
persistent identity: range2469977
display id: range2469977
version: 1
direction: direction/forward
top level: BBa_K1689017
start: 37
end: 54
orientation: inline

dCas9
type: SequenceAnnotation
persistent identity: annotation2469982
display id: annotation2469982
version: 1
top level: BBa_K1689017
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1689017/annotation2469982/range2469982/1

https://synbiohub.org/public/igem/BBa_K1689017/annotation2469982/range2469982/1
type: Range
persistent identity: range2469982
display id: range2469982
version: 1
direction: direction/forward
top level: BBa_K1689017
start: 298
end: 4398
orientation: inline

F3-dCas9
type: SequenceAnnotation
persistent identity: annotation2469975
display id: annotation2469975
version: 1
top level: BBa_K1689017
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1689017/annotation2469975/range2469975/1

https://synbiohub.org/public/igem/BBa_K1689017/annotation2469975/range2469975/1
type: Range
persistent identity: range2469975
display id: range2469975
version: 1
direction: direction/forward
top level: BBa_K1689017
start: 34
end: 4401
orientation: inline

random spacer
type: SequenceAnnotation
persistent identity: annotation2477789
display id: annotation2477789
version: 1
top level: BBa_K1689017
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1689017/annotation2477789/range2477789/1

https://synbiohub.org/public/igem/BBa_K1689017/annotation2477789/range2477789/1
type: Range
persistent identity: range2477789
display id: range2477789
version: 1
direction: direction/forward
top level: BBa_K1689017
start: 1
end: 33
orientation: inline

random spacer
type: SequenceAnnotation
persistent identity: annotation2477792
display id: annotation2477792
version: 1
top level: BBa_K1689017
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1689017/annotation2477792/range2477792/1

https://synbiohub.org/public/igem/BBa_K1689017/annotation2477792/range2477792/1
type: Range
persistent identity: range2477792
display id: range2477792
version: 1
direction: direction/forward
top level: BBa_K1689017
start: 4402
end: 4472
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2477790
display id: annotation2477790
version: 1
top level: BBa_K1689017
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1689017/annotation2477790/range2477790/1

https://synbiohub.org/public/igem/BBa_K1689017/annotation2477790/range2477790/1
type: Range
persistent identity: range2477790
display id: range2477790
version: 1
direction: direction/forward
top level: BBa_K1689017
start: 34
end: 36
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2477791
display id: annotation2477791
version: 1
top level: BBa_K1689017
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1689017/annotation2477791/range2477791/1

https://synbiohub.org/public/igem/BBa_K1689017/annotation2477791/range2477791/1
type: Range
persistent identity: range2477791
display id: range2477791
version: 1
direction: direction/forward
top level: BBa_K1689017
start: 4398
end: 4401
orientation: inline

GGGGS*2 Linker
type: SequenceAnnotation
persistent identity: annotation2469979
display id: annotation2469979
version: 1
top level: BBa_K1689017
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1689017/annotation2469979/range2469979/1

https://synbiohub.org/public/igem/BBa_K1689017/annotation2469979/range2469979/1
type: Range
persistent identity: range2469979
display id: range2469979
version: 1
direction: direction/forward
top level: BBa_K1689017
start: 268
end: 297
orientation: inline

BBa_K1598003
type: ComponentDefinition
persistent identity: BBa K1598003
display id: BBa_K1598003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS-ChAT-6xHis-Terminator
creator: Marta Napiorkowska
created: 2015-08-26T11:00:00Z
modified: 2015-08-28T10:29:27Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2015_
m user id: 27259
owner id: 27180
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This part consists of B0034 RBS, human choline acetyltransferase (CHAT) gene with C-terminal His tag, and B0015 terminator.
mutable notes: - human CHAT gene (UniProt accession no: P28329) was codon-optimized for E.coli and provided with C-terminal 6xHis tag - due to its length, the sequence was synthesized as two gBlock fragments with 30 bp overlap between each other and with 20 bp overlaps with respective PSB1C3 ends - the construct was cloned into PSB1C3 using Gibson Assembly
mutable provenance: DNA synthesis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: RBS B0034
sequence annotation: CHAT
sequence annotation: Terminator B0015
sequence: https://synbiohub.org/public/igem/BBa_K1598003_sequence/1

RBS B0034
type: SequenceAnnotation
persistent identity: annotation2438652
display id: annotation2438652
version: 1
top level: BBa_K1598003
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1598003/annotation2438652/range2438652/1

https://synbiohub.org/public/igem/BBa_K1598003/annotation2438652/range2438652/1
type: Range
persistent identity: range2438652
display id: range2438652
version: 1
direction: direction/forward
top level: BBa_K1598003
start: 1
end: 12
orientation: inline

CHAT
type: SequenceAnnotation
persistent identity: annotation2438653
display id: annotation2438653
version: 1
top level: BBa_K1598003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1598003/annotation2438653/range2438653/1

https://synbiohub.org/public/igem/BBa_K1598003/annotation2438653/range2438653/1
type: Range
persistent identity: range2438653
display id: range2438653
version: 1
direction: direction/forward
top level: BBa_K1598003
start: 19
end: 1932
orientation: inline

Terminator B0015
type: SequenceAnnotation
persistent identity: annotation2438654
display id: annotation2438654
version: 1
top level: BBa_K1598003
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1598003/annotation2438654/range2438654/1

https://synbiohub.org/public/igem/BBa_K1598003/annotation2438654/range2438654/1
type: Range
persistent identity: range2438654
display id: range2438654
version: 1
direction: direction/forward
top level: BBa_K1598003
start: 1941
end: 2069
orientation: inline

T7 express
type: ComponentDefinition
persistent identity: BBa K1639012
display id: BBa_K1639012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 expression system for pSB1C3
creator: Nurgeldi Bazarov
created: 2015-09-14T11:00:00Z
modified: 2015-09-18T06:55:31Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2056_
m user id: 20835
owner id: 24789
owning group id: 9
status: status/Planning
bookmark: true
mutable description: In our project we needed to express two different genes at the same bacteria, but unfortunately because of high repeats of sequences gene synthesizing company couldn't synthesize it. To solve this we just divided our genes into different plasmids and cotransformed it into same bacteria. For coexpression of genes from different plasmids origins have to be compatible, like ColA and ColE1. pSB1C3 possess ColE1 origin of replication. So it's compatible for expression with plasmid containg ColA origin of replication. But it doesn't contain expression machinery like promoters, terminators. We inserted this part to modify pSB1C3 so that it can be used as expression vector.
mutable notes: We inserted BamH1 and Sal1 cut sites between T7 promoter and T7 terminator. This part also contain RBS. Any other team can use this part to modify vectors like pSB1C3 to add T7 expression feature. Just clone it with enzymes at prefix and suffix.
mutable provenance: It is combination of T7 promoter-terminator with sequences containing restriction enzyme cut sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: T7 bacteriophage RBS
sequence annotation: T7 terminator
sequence annotation: T7 promoter
sequence: https://synbiohub.org/public/igem/BBa_K1639012_sequence/1

T7 bacteriophage RBS
type: SequenceAnnotation
persistent identity: annotation2457603
display id: annotation2457603
version: 1
top level: T7 express
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1639012/annotation2457603/range2457603/1

https://synbiohub.org/public/igem/BBa_K1639012/annotation2457603/range2457603/1
type: Range
persistent identity: range2457603
display id: range2457603
version: 1
direction: direction/forward
top level: T7 express
start: 35
end: 57
orientation: inline

T7 terminator
type: SequenceAnnotation
persistent identity: annotation2457604
display id: annotation2457604
version: 1
top level: T7 express
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1639012/annotation2457604/range2457604/1

https://synbiohub.org/public/igem/BBa_K1639012/annotation2457604/range2457604/1
type: Range
persistent identity: range2457604
display id: range2457604
version: 1
direction: direction/forward
top level: T7 express
start: 124
end: 171
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2457602
display id: annotation2457602
version: 1
top level: T7 express
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1639012/annotation2457602/range2457602/1

https://synbiohub.org/public/igem/BBa_K1639012/annotation2457602/range2457602/1
type: Range
persistent identity: range2457602
display id: range2457602
version: 1
direction: direction/forward
top level: T7 express
start: 1
end: 19
orientation: inline

p(tetR)
type: ComponentDefinition
persistent identity: BBa R0040
display id: BBa_R0040
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TetR repressible promoter
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Sequence for pTet inverting regulator driven by the TetR protein.
mutable notes: BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.
mutable provenance: Lutz, R., Bujard, H., Nucleic Acids Research (1997) 25, 1203-1210.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: TetR 2
sequence annotation: BBa_R0040
sequence annotation: TetR 1
sequence annotation: -10
sequence annotation: -35
sequence: https://synbiohub.org/public/igem/BBa_R0040_sequence/1

TetR 2
type: SequenceAnnotation
persistent identity: annotation1986786
display id: annotation1986786
version: 1
top level: p(tetR)
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1

https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
type: Range
persistent identity: range1986786
display id: range1986786
version: 1
direction: direction/forward
top level: p(tetR)
start: 26
end: 44
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1986784
display id: annotation1986784
version: 1
top level: p(tetR)
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1

https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
type: Range
persistent identity: range1986784
display id: range1986784
version: 1
direction: direction/forward
top level: p(tetR)
start: 1
end: 54
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1986783
display id: annotation1986783
version: 1
top level: p(tetR)
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1

https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
type: Range
persistent identity: range1986783
display id: range1986783
version: 1
direction: direction/forward
top level: p(tetR)
start: 1
end: 19
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986787
display id: annotation1986787
version: 1
top level: p(tetR)
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1

https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
type: Range
persistent identity: range1986787
display id: range1986787
version: 1
direction: direction/forward
top level: p(tetR)
start: 43
end: 48
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1986785
display id: annotation1986785
version: 1
top level: p(tetR)
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1

https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
type: Range
persistent identity: range1986785
display id: range1986785
version: 1
direction: direction/forward
top level: p(tetR)
start: 20
end: 25
orientation: inline

BBa_K1602056
type: ComponentDefinition
persistent identity: BBa K1602056
display id: BBa_K1602056
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0034-yjhG-B0034-yqhD
creator: Leon Kraus, Jonathan Pletzer-Zelgert, Jonas Sindlinger, Carmen Sakas Gandullo, Sebastian Holzner, Elena Tonchevska, Mirjam Haun
created: 2015-09-15T11:00:00Z
modified: 2015-09-18T06:35:45Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2019_
m user id: 26220
owner id: 25990
owning group id: 9
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0034
component: BBa_K1602013
component: BBa_K1602012
sequence annotation: BBa_K1602012
sequence annotation: BBa_B0034
sequence annotation: BBa_K1602013
sequence: https://synbiohub.org/public/igem/BBa_K1602056_sequence/1

BBa_B0034
type: Component
persistent identity: component2462944
display id: component2462944
version: 1
top level: BBa_K1602056
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: Component
persistent identity: component2462941
display id: component2462941
version: 1
top level: BBa_K1602056
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1602013
type: Component
persistent identity: component2462945
display id: component2462945
version: 1
top level: BBa_K1602056
access: public
definition: BBa_K1602013

BBa_K1602012
type: Component
persistent identity: component2462942
display id: component2462942
version: 1
top level: BBa_K1602056
access: public
definition: BBa_K1602012

BBa_K1602012
type: SequenceAnnotation
persistent identity: annotation2462942
display id: annotation2462942
version: 1
top level: BBa_K1602056
location: https://synbiohub.org/public/igem/BBa_K1602056/annotation2462942/range2462942/1
component: BBa_K1602012

https://synbiohub.org/public/igem/BBa_K1602056/annotation2462942/range2462942/1
type: Range
persistent identity: range2462942
display id: range2462942
version: 1
direction: direction/reverse
top level: BBa_K1602056
start: 19
end: 1986
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2462941
display id: annotation2462941
version: 1
top level: BBa_K1602056
location: https://synbiohub.org/public/igem/BBa_K1602056/annotation2462941/range2462941/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1602056/annotation2462941/range2462941/1
type: Range
persistent identity: range2462941
display id: range2462941
version: 1
direction: direction/reverse
top level: BBa_K1602056
start: 1
end: 12
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2462944
display id: annotation2462944
version: 1
top level: BBa_K1602056
location: https://synbiohub.org/public/igem/BBa_K1602056/annotation2462944/range2462944/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1602056/annotation2462944/range2462944/1
type: Range
persistent identity: range2462944
display id: range2462944
version: 1
direction: direction/reverse
top level: BBa_K1602056
start: 1995
end: 2006
orientation: inline

BBa_K1602013
type: SequenceAnnotation
persistent identity: annotation2462945
display id: annotation2462945
version: 1
top level: BBa_K1602056
location: https://synbiohub.org/public/igem/BBa_K1602056/annotation2462945/range2462945/1
component: BBa_K1602013

https://synbiohub.org/public/igem/BBa_K1602056/annotation2462945/range2462945/1
type: Range
persistent identity: range2462945
display id: range2462945
version: 1
direction: direction/reverse
top level: BBa_K1602056
start: 2013
end: 3176
orientation: inline

Si-tag1+2
type: ComponentDefinition
persistent identity: BBa K1592010
display id: BBa_K1592010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LIP2 prepro + E. coli ribosomal protein L2 (1-202aa)+ YLcwp3 Fusion
creator: Shuyan Tang
created: 2015-09-05T11:00:00Z
modified: 2015-09-18T09:31:56Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2009_
m user id: 20267
owner id: 20267
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This is the cell display system of Yarrowia lipolytica, composed of LIP prepro, interest protein, and YLcwp3. LIP prepro is signal peptide used to secrete the interest protein out of the cell, and the YLcwp3 is the anchor domain binding the interest protein to the cell wall of yeast. We use this system to display silica-tag and test its binding characteristics. E.coli ribosomal protein L2 was found to bind tightly to silicon particles, which have surfaces that are oxidized to silica. This L2 silica-binding tag, called the ??????Si-tag,?????? can be used for one-step targeting of functional proteins on silica surfaces. The silica-binding domains of E. coli L2 was mapped to amino acids 1???60, 61-202 and 203???273, called Si-tag1, Si-tag2,Si-tag3. We respectively test the silica-binding characteristics of this three regions and their combinations..
mutable notes: In order to replace the domains more conveniently, we respectivey added BamHI, SalI, NdeI between LIP2 prepro, E. coli ribosomal protein L2, GS linker and YLcwp3.
mutable provenance: E. coli ribosomal protein L2 was synthesized by IDT, LIP2 prepro and YLcwp3 were cloning from the plasmid JMP62, and we fused this three sequences.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: GS linker
sequence annotation: LIP2 prepro
sequence annotation: BamHI
sequence annotation: YLcwp3
sequence annotation: NdeI
sequence annotation: Si-tag 12
sequence annotation: SalI
sequence: https://synbiohub.org/public/igem/BBa_K1592010_sequence/1

GS linker
type: SequenceAnnotation
persistent identity: annotation2456138
display id: annotation2456138
version: 1
top level: Si-tag1+2
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1592010/annotation2456138/range2456138/1

https://synbiohub.org/public/igem/BBa_K1592010/annotation2456138/range2456138/1
type: Range
persistent identity: range2456138
display id: range2456138
version: 1
direction: direction/forward
top level: Si-tag1+2
start: 718
end: 762
orientation: inline

LIP2 prepro
type: SequenceAnnotation
persistent identity: annotation2456128
display id: annotation2456128
version: 1
top level: Si-tag1+2
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1592010/annotation2456128/range2456128/1

https://synbiohub.org/public/igem/BBa_K1592010/annotation2456128/range2456128/1
type: Range
persistent identity: range2456128
display id: range2456128
version: 1
direction: direction/forward
top level: Si-tag1+2
start: 1
end: 99
orientation: inline

BamHI
type: SequenceAnnotation
persistent identity: annotation2456129
display id: annotation2456129
version: 1
top level: Si-tag1+2
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1592010/annotation2456129/range2456129/1

https://synbiohub.org/public/igem/BBa_K1592010/annotation2456129/range2456129/1
type: Range
persistent identity: range2456129
display id: range2456129
version: 1
direction: direction/forward
top level: Si-tag1+2
start: 100
end: 105
orientation: inline

YLcwp3
type: SequenceAnnotation
persistent identity: annotation2456144
display id: annotation2456144
version: 1
top level: Si-tag1+2
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1592010/annotation2456144/range2456144/1

https://synbiohub.org/public/igem/BBa_K1592010/annotation2456144/range2456144/1
type: Range
persistent identity: range2456144
display id: range2456144
version: 1
direction: direction/forward
top level: Si-tag1+2
start: 769
end: 1134
orientation: inline

NdeI
type: SequenceAnnotation
persistent identity: annotation2456141
display id: annotation2456141
version: 1
top level: Si-tag1+2
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1592010/annotation2456141/range2456141/1

https://synbiohub.org/public/igem/BBa_K1592010/annotation2456141/range2456141/1
type: Range
persistent identity: range2456141
display id: range2456141
version: 1
direction: direction/forward
top level: Si-tag1+2
start: 763
end: 768
orientation: inline

Si-tag 12
type: SequenceAnnotation
persistent identity: annotation2456130
display id: annotation2456130
version: 1
top level: Si-tag1+2
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1592010/annotation2456130/range2456130/1

https://synbiohub.org/public/igem/BBa_K1592010/annotation2456130/range2456130/1
type: Range
persistent identity: range2456130
display id: range2456130
version: 1
direction: direction/forward
top level: Si-tag1+2
start: 106
end: 711
orientation: inline

SalI
type: SequenceAnnotation
persistent identity: annotation2456137
display id: annotation2456137
version: 1
top level: Si-tag1+2
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1592010/annotation2456137/range2456137/1

https://synbiohub.org/public/igem/BBa_K1592010/annotation2456137/range2456137/1
type: Range
persistent identity: range2456137
display id: range2456137
version: 1
direction: direction/forward
top level: Si-tag1+2
start: 712
end: 717
orientation: inline

BBa_K1674002
type: ComponentDefinition
persistent identity: BBa K1674002
display id: BBa_K1674002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pT7 - RBS - 3αHSD
creator: Adi Yannai
created: 2015-09-14T11:00:00Z
modified: 2015-09-17T03:07:01Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2092_
m user id: 25765
owner id: 25765
owning group id: 9
status: status/Planning
bookmark: false
mutable description: pT7 - 3αHSD
mutable notes: pT7 - 3αHSD
mutable provenance: pT7 - 3αHSD
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1674001
component: BBa_K1321338
sequence annotation: BBa_K1321338
sequence annotation: BBa_K1674001
sequence: https://synbiohub.org/public/igem/BBa_K1674002_sequence/1

BBa_K1674001
type: Component
persistent identity: component2467114
display id: component2467114
version: 1
top level: BBa_K1674002
access: public
definition: 3alpha-HSD

BBa_K1321338
type: Component
persistent identity: component2467113
display id: component2467113
version: 1
top level: BBa_K1674002
access: public
definition: https://synbiohub.org/public/igem/BBa_K1321338/1

BBa_K1321338
type: SequenceAnnotation
persistent identity: annotation2467113
display id: annotation2467113
version: 1
top level: BBa_K1674002
location: https://synbiohub.org/public/igem/BBa_K1674002/annotation2467113/range2467113/1
component: BBa_K1321338

https://synbiohub.org/public/igem/BBa_K1674002/annotation2467113/range2467113/1
type: Range
persistent identity: range2467113
display id: range2467113
version: 1
direction: direction/reverse
top level: BBa_K1674002
start: 1
end: 43
orientation: inline

BBa_K1674001
type: SequenceAnnotation
persistent identity: annotation2467114
display id: annotation2467114
version: 1
top level: BBa_K1674002
location: https://synbiohub.org/public/igem/BBa_K1674002/annotation2467114/range2467114/1
component: BBa_K1674001

https://synbiohub.org/public/igem/BBa_K1674002/annotation2467114/range2467114/1
type: Range
persistent identity: range2467114
display id: range2467114
version: 1
direction: direction/reverse
top level: BBa_K1674002
start: 50
end: 1018
orientation: inline

BBa_K1689007
type: ComponentDefinition
persistent identity: BBa K1689007
display id: BBa_K1689007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: dCas9-C-luc
creator: ZHANG Yihao
created: 2015-09-15T11:00:00Z
modified: 2015-09-27T01:02:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2107_
m user id: 26531
owner id: 26434
owning group id: 9
status: status/Planning
bookmark: false
mutable description: the coding sequence of the fusion protein of dCas and the CFluc of the luciferase
mutable notes: Goldengate
mutable provenance: E.coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: start codon
sequence annotation: rrnB T1 terminator
sequence annotation: stop codon
sequence annotation: random spacer
sequence annotation: dCas9
sequence annotation: CFluc398
sequence annotation: B0034
sequence annotation: GGGGSGGGGS linker
sequence: https://synbiohub.org/public/igem/BBa_K1689007_sequence/1

start codon
type: SequenceAnnotation
persistent identity: annotation2473593
display id: annotation2473593
version: 1
top level: BBa_K1689007
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1689007/annotation2473593/range2473593/1

https://synbiohub.org/public/igem/BBa_K1689007/annotation2473593/range2473593/1
type: Range
persistent identity: range2473593
display id: range2473593
version: 1
direction: direction/forward
top level: BBa_K1689007
start: 78
end: 80
orientation: inline

rrnB T1 terminator
type: SequenceAnnotation
persistent identity: annotation2469599
display id: annotation2469599
version: 1
top level: BBa_K1689007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1689007/annotation2469599/range2469599/1

https://synbiohub.org/public/igem/BBa_K1689007/annotation2469599/range2469599/1
type: Range
persistent identity: range2469599
display id: range2469599
version: 1
direction: direction/forward
top level: BBa_K1689007
start: 4686
end: 4764
orientation: inline

stop codon
type: SequenceAnnotation
persistent identity: annotation2473597
display id: annotation2473597
version: 1
top level: BBa_K1689007
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1689007/annotation2473597/range2473597/1

https://synbiohub.org/public/igem/BBa_K1689007/annotation2473597/range2473597/1
type: Range
persistent identity: range2473597
display id: range2473597
version: 1
direction: direction/forward
top level: BBa_K1689007
start: 4668
end: 4670
orientation: inline

random spacer
type: SequenceAnnotation
persistent identity: annotation2477746
display id: annotation2477746
version: 1
top level: BBa_K1689007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1689007/annotation2477746/range2477746/1

https://synbiohub.org/public/igem/BBa_K1689007/annotation2477746/range2477746/1
type: Range
persistent identity: range2477746
display id: range2477746
version: 1
direction: direction/forward
top level: BBa_K1689007
start: 4765
end: 4841
orientation: inline

dCas9
type: SequenceAnnotation
persistent identity: annotation2473594
display id: annotation2473594
version: 1
top level: BBa_K1689007
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1689007/annotation2473594/range2473594/1

https://synbiohub.org/public/igem/BBa_K1689007/annotation2473594/range2473594/1
type: Range
persistent identity: range2473594
display id: range2473594
version: 1
direction: direction/forward
top level: BBa_K1689007
start: 81
end: 4181
orientation: inline

CFluc398
type: SequenceAnnotation
persistent identity: annotation2473596
display id: annotation2473596
version: 1
top level: BBa_K1689007
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1689007/annotation2473596/range2473596/1

https://synbiohub.org/public/igem/BBa_K1689007/annotation2473596/range2473596/1
type: Range
persistent identity: range2473596
display id: range2473596
version: 1
direction: direction/forward
top level: BBa_K1689007
start: 4212
end: 4667
orientation: inline

random spacer
type: SequenceAnnotation
persistent identity: annotation2477747
display id: annotation2477747
version: 1
top level: BBa_K1689007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1689007/annotation2477747/range2477747/1

https://synbiohub.org/public/igem/BBa_K1689007/annotation2477747/range2477747/1
type: Range
persistent identity: range2477747
display id: range2477747
version: 1
direction: direction/forward
top level: BBa_K1689007
start: 1
end: 59
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2473592
display id: annotation2473592
version: 1
top level: BBa_K1689007
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1689007/annotation2473592/range2473592/1

https://synbiohub.org/public/igem/BBa_K1689007/annotation2473592/range2473592/1
type: Range
persistent identity: range2473592
display id: range2473592
version: 1
direction: direction/forward
top level: BBa_K1689007
start: 60
end: 77
orientation: inline

GGGGSGGGGS linker
type: SequenceAnnotation
persistent identity: annotation2473595
display id: annotation2473595
version: 1
top level: BBa_K1689007
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1689007/annotation2473595/range2473595/1

https://synbiohub.org/public/igem/BBa_K1689007/annotation2473595/range2473595/1
type: Range
persistent identity: range2473595
display id: range2473595
version: 1
direction: direction/forward
top level: BBa_K1689007
start: 4182
end: 4211
orientation: inline

BBa_K1795007
type: ComponentDefinition
persistent identity: BBa K1795007
display id: BBa_K1795007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23101 gRNA under R0040
creator: Andrew D Halleran, Joseph L Maniaci
created: 2015-09-16T11:00:00Z
modified: 2015-09-17T09:43:40Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2220_
m user id: 27446
owner id: 27446
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This sgRNA sequence was designed to target the Promoter Bba_J23101 with the N20 sequence tttacagctagctcagtcct . If this gRNA is expressed in a cell with a form of the dCas9 protein, the protein will be targeted to the N20 sequence and repress gene expression. The part is under the control of the promoter Bba_R0040. Promoter Bba_R0040 was chosen because it does not contain a PAM and therefore cannot be inadvertently repressed by dCas9 due to unintentional homology in the N20 sequence. Expression of this part can be repressed if TetR is expressed in the cell without Tetracycline present. If TetR and Tetracycline (or its analog aTc) are both present, the part will be expressed.
mutable notes: We made sure to use a different promoter for this part than the one which the part targets.
mutable provenance: The basic template for the sgRNA sequence was taken from the supplementary information of Qi LS, Larson MH, Gilbert LA, et al. Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression. Cell. 2013;152(5):1173-1183. doi:10.1016/j.cell.2013.02.022.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1795100
component: BBa_K1795107
component: BBa_B0015
component: BBa_R0040
sequence annotation: BBa_R0040
sequence annotation: BBa_K1795100
sequence annotation: BBa_K1795107
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K1795007_sequence/1

BBa_K1795100
type: Component
persistent identity: component2467789
display id: component2467789
version: 1
top level: BBa_K1795007
access: public
definition: https://synbiohub.org/public/igem/BBa_K1795100/1

BBa_K1795107
type: Component
persistent identity: component2467788
display id: component2467788
version: 1
top level: BBa_K1795007
access: public
definition: https://synbiohub.org/public/igem/BBa_K1795107/1

BBa_B0015
type: Component
persistent identity: component2467796
display id: component2467796
version: 1
top level: BBa_K1795007
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_R0040
type: Component
persistent identity: component2467783
display id: component2467783
version: 1
top level: BBa_K1795007
access: public
definition: p(tetR)

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2467783
display id: annotation2467783
version: 1
top level: BBa_K1795007
location: https://synbiohub.org/public/igem/BBa_K1795007/annotation2467783/range2467783/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K1795007/annotation2467783/range2467783/1
type: Range
persistent identity: range2467783
display id: range2467783
version: 1
direction: direction/forward
top level: BBa_K1795007
start: 1
end: 54
orientation: inline

BBa_K1795100
type: SequenceAnnotation
persistent identity: annotation2467789
display id: annotation2467789
version: 1
top level: BBa_K1795007
location: https://synbiohub.org/public/igem/BBa_K1795007/annotation2467789/range2467789/1
component: BBa_K1795100

https://synbiohub.org/public/igem/BBa_K1795007/annotation2467789/range2467789/1
type: Range
persistent identity: range2467789
display id: range2467789
version: 1
direction: direction/reverse
top level: BBa_K1795007
start: 75
end: 156
orientation: inline

BBa_K1795107
type: SequenceAnnotation
persistent identity: annotation2467788
display id: annotation2467788
version: 1
top level: BBa_K1795007
location: https://synbiohub.org/public/igem/BBa_K1795007/annotation2467788/range2467788/1
component: BBa_K1795107

https://synbiohub.org/public/igem/BBa_K1795007/annotation2467788/range2467788/1
type: Range
persistent identity: range2467788
display id: range2467788
version: 1
direction: direction/reverse
top level: BBa_K1795007
start: 55
end: 74
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2467796
display id: annotation2467796
version: 1
top level: BBa_K1795007
location: https://synbiohub.org/public/igem/BBa_K1795007/annotation2467796/range2467796/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1795007/annotation2467796/range2467796/1
type: Range
persistent identity: range2467796
display id: range2467796
version: 1
direction: direction/reverse
top level: BBa_K1795007
start: 157
end: 285
orientation: inline

aes-SH3lig
type: ComponentDefinition
persistent identity: BBa K1602034
display id: BBa_K1602034
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: aes-SH3lig
creator: Jasmin Weber, Michelle Zoeller, Marcus Geissler, Stefan Bohn, Simon Linder, Sebastian Barthel
created: 2015-09-15T11:00:00Z
modified: 2015-09-16T07:54:44Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2019_
m user id: 22022
owner id: 22022
owning group id: 9
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1602034_sequence/1

BBa_K1682004
type: ComponentDefinition
persistent identity: BBa K1682004
display id: BBa_K1682004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PkdpF[-15, T>G]
creator: iGEM15_HKUST-Rice
created: 2015-08-09T11:00:00Z
modified: 2015-09-18T09:16:31Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2100_
m user id: 8758
owner id: 21235
owning group id: 9
status: status/Planning
bookmark: false
mutable description: kdpFp[-15, T>G]
mutable notes: kdpFp[-15, T>G]
mutable provenance: kdpFp[-15, T>G]
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: phospho-KdpE binding site
sequence annotation: -10
sequence annotation: -35
sequence annotation: EcoRI Illegal site removed T->G
sequence: https://synbiohub.org/public/igem/BBa_K1682004_sequence/1

phospho-KdpE binding site
type: SequenceAnnotation
persistent identity: annotation2473581
display id: annotation2473581
version: 1
top level: BBa_K1682004
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1682004/annotation2473581/range2473581/1

https://synbiohub.org/public/igem/BBa_K1682004/annotation2473581/range2473581/1
type: Range
persistent identity: range2473581
display id: range2473581
version: 1
direction: direction/forward
top level: BBa_K1682004
start: 8
end: 24
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2473582
display id: annotation2473582
version: 1
top level: BBa_K1682004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1682004/annotation2473582/range2473582/1

https://synbiohub.org/public/igem/BBa_K1682004/annotation2473582/range2473582/1
type: Range
persistent identity: range2473582
display id: range2473582
version: 1
direction: direction/forward
top level: BBa_K1682004
start: 69
end: 74
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2473584
display id: annotation2473584
version: 1
top level: BBa_K1682004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1682004/annotation2473584/range2473584/1

https://synbiohub.org/public/igem/BBa_K1682004/annotation2473584/range2473584/1
type: Range
persistent identity: range2473584
display id: range2473584
version: 1
direction: direction/forward
top level: BBa_K1682004
start: 42
end: 47
orientation: inline

EcoRI Illegal site removed T->G
type: SequenceAnnotation
persistent identity: annotation2475215
display id: annotation2475215
version: 1
top level: BBa_K1682004
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K1682004/annotation2475215/range2475215/1

https://synbiohub.org/public/igem/BBa_K1682004/annotation2475215/range2475215/1
type: Range
persistent identity: range2475215
display id: range2475215
version: 1
direction: direction/forward
top level: BBa_K1682004
start: 65
end: 66
orientation: inline

BBa_K1758301
type: ComponentDefinition
persistent identity: BBa K1758301
display id: BBa_K1758301
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: His-tagged arsenic repressor (ArsR) under control of the T7 promoter
creator: Team Bielefeld-CeBiTec 2015
created: 2015-08-21T11:00:00Z
modified: 2015-09-18T04:46:36Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2180_
m user id: 27935
owner id: 26587
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This part can be used for expression and purification of the arsenic repressor ArsR.
mutable notes: Created using Gibson Assembly.
mutable provenance: Based on BBa_J33201.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: arsR
sequence annotation: T7 promoter + RBS
sequence annotation: his-tag
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_K1758301_sequence/1

arsR
type: SequenceAnnotation
persistent identity: annotation2436873
display id: annotation2436873
version: 1
top level: BBa_K1758301
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1758301/annotation2436873/range2436873/1

https://synbiohub.org/public/igem/BBa_K1758301/annotation2436873/range2436873/1
type: Range
persistent identity: range2436873
display id: range2436873
version: 1
direction: direction/forward
top level: BBa_K1758301
start: 41
end: 391
orientation: inline

T7 promoter + RBS
type: SequenceAnnotation
persistent identity: annotation2436876
display id: annotation2436876
version: 1
top level: BBa_K1758301
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1758301/annotation2436876/range2436876/1

https://synbiohub.org/public/igem/BBa_K1758301/annotation2436876/range2436876/1
type: Range
persistent identity: range2436876
display id: range2436876
version: 1
direction: direction/forward
top level: BBa_K1758301
start: 1
end: 32
orientation: inline

his-tag
type: SequenceAnnotation
persistent identity: annotation2436874
display id: annotation2436874
version: 1
top level: BBa_K1758301
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1758301/annotation2436874/range2436874/1

https://synbiohub.org/public/igem/BBa_K1758301/annotation2436874/range2436874/1
type: Range
persistent identity: range2436874
display id: range2436874
version: 1
direction: direction/forward
top level: BBa_K1758301
start: 392
end: 409
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2436875
display id: annotation2436875
version: 1
top level: BBa_K1758301
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1758301/annotation2436875/range2436875/1

https://synbiohub.org/public/igem/BBa_K1758301/annotation2436875/range2436875/1
type: Range
persistent identity: range2436875
display id: range2436875
version: 1
direction: direction/forward
top level: BBa_K1758301
start: 410
end: 412
orientation: inline

BBa_K1847004
type: ComponentDefinition
persistent identity: BBa K1847004
display id: BBa_K1847004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23118-lldRO1-lldRO2
creator: Anna Fomitcheva Khartchenko
created: 2015-08-25T11:00:00Z
modified: 2015-08-26T04:23:26Z
discontinued: false
dominant: false
group u list: _2273_
m user id: 26442
owner id: 26442
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Collection of promoters regulated by LldR.
mutable provenance: Synthetic synthesis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: J23118
sequence annotation: lldRO1
sequence annotation: lldRO2
sequence: https://synbiohub.org/public/igem/BBa_K1847004_sequence/1

J23118
type: SequenceAnnotation
persistent identity: annotation2438054
display id: annotation2438054
version: 1
top level: BBa_K1847004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1847004/annotation2438054/range2438054/1

https://synbiohub.org/public/igem/BBa_K1847004/annotation2438054/range2438054/1
type: Range
persistent identity: range2438054
display id: range2438054
version: 1
direction: direction/forward
top level: BBa_K1847004
start: 1
end: 35
orientation: inline

lldRO1
type: SequenceAnnotation
persistent identity: annotation2438055
display id: annotation2438055
version: 1
top level: BBa_K1847004
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K1847004/annotation2438055/range2438055/1

https://synbiohub.org/public/igem/BBa_K1847004/annotation2438055/range2438055/1
type: Range
persistent identity: range2438055
display id: range2438055
version: 1
direction: direction/forward
top level: BBa_K1847004
start: 44
end: 60
orientation: inline

lldRO2
type: SequenceAnnotation
persistent identity: annotation2438056
display id: annotation2438056
version: 1
top level: BBa_K1847004
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K1847004/annotation2438056/range2438056/1

https://synbiohub.org/public/igem/BBa_K1847004/annotation2438056/range2438056/1
type: Range
persistent identity: range2438056
display id: range2438056
version: 1
direction: direction/forward
top level: BBa_K1847004
start: 61
end: 77
orientation: inline

BBa_K1632012
type: ComponentDefinition
persistent identity: BBa K1632012
display id: BBa_K1632012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PBAD/araC_rbs_fimB(wild-type)
creator: Riku Shinohara
created: 2015-08-29T11:00:00Z
modified: 2015-09-18T09:40:16Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2049_
m user id: 24760
owner id: 22687
owning group id: 9
status: status/Planning
bookmark: true
mutable description: a
mutable notes: a
mutable provenance: a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1632010
component: BBa_I0500
component: BBa_B0034
sequence annotation: BBa_K1632010
sequence annotation: BBa_I0500
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K1632012_sequence/1

BBa_K1632010
type: Component
persistent identity: component2440137
display id: component2440137
version: 1
top level: BBa_K1632012
access: public
definition: BBa_K1632010

BBa_I0500
type: Component
persistent identity: component2440134
display id: component2440134
version: 1
top level: BBa_K1632012
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_B0034
type: Component
persistent identity: component2440136
display id: component2440136
version: 1
top level: BBa_K1632012
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1632010
type: SequenceAnnotation
persistent identity: annotation2440137
display id: annotation2440137
version: 1
top level: BBa_K1632012
location: https://synbiohub.org/public/igem/BBa_K1632012/annotation2440137/range2440137/1
component: BBa_K1632010

https://synbiohub.org/public/igem/BBa_K1632012/annotation2440137/range2440137/1
type: Range
persistent identity: range2440137
display id: range2440137
version: 1
direction: direction/reverse
top level: BBa_K1632012
start: 1237
end: 1839
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2440134
display id: annotation2440134
version: 1
top level: BBa_K1632012
location: https://synbiohub.org/public/igem/BBa_K1632012/annotation2440134/range2440134/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_K1632012/annotation2440134/range2440134/1
type: Range
persistent identity: range2440134
display id: range2440134
version: 1
direction: direction/reverse
top level: BBa_K1632012
start: 1
end: 1210
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2440136
display id: annotation2440136
version: 1
top level: BBa_K1632012
location: https://synbiohub.org/public/igem/BBa_K1632012/annotation2440136/range2440136/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1632012/annotation2440136/range2440136/1
type: Range
persistent identity: range2440136
display id: range2440136
version: 1
direction: direction/reverse
top level: BBa_K1632012
start: 1219
end: 1230
orientation: inline

Si-tag1+2+
type: ComponentDefinition
persistent identity: BBa K1592014
display id: BBa_K1592014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LIP prepro + E. coli ribosomal protein L2 (1-273aa) + YLcwp3
creator: Shuyan Tang
created: 2015-09-07T11:00:00Z
modified: 2015-09-18T09:37:10Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2009_
m user id: 20267
owner id: 20267
owning group id: 9
status: status/Planning
bookmark: true
mutable description: Long description: __NOTOC__ BBa_K1592008 short This is the cell display system of Yarrowia lipolytica, composed of LIP prepro, interest protein, and YLcwp3.LIP prepro is signal peptide used to secrete the interest protein out of the cell, and the YLcwp3 is the anchor domain binding the interest protein to the cell wall of yeast.We use this system to display silica-tag and test its binding characteristics. Here we use this system to display Silica-tag on cell wall of Yarrowia lipolytica to binding the silica. E.coli ribosomal protein L2 was found to bind tightly to silicon particles, which have surfaces that are oxidized to silica. This L2 silica-binding tag, called the 'Si-tag', can be used for one-step targeting of functional proteins on silica surfaces. The silica-binding domains of E. coli L2 was mapped to amino acids 1???60, 61-202 and 203???273, called Si-tag1, Si-tag2 and Si-tag3. We respectively test the silica-binding characteristics of this three regions and their combinations.
mutable notes: In order to replace the domains more conveniently, we respectivey added BamHI, SalI, NdeI between LIP2 prepro, E. coli ribosomal protein L2, GS linker and YLcwp3
mutable provenance: E. coli ribosomal protein L2 was synthesized by IDT, LIP2 prepro and YLcwp3 were cloning from the plasmid JMP62, and we fused this three sequences.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: GS Linker
sequence annotation: Si-tag 123
sequence annotation: YLcwp3
sequence annotation: SalI
sequence annotation: LIP2 prepro
sequence annotation: BamHI
sequence annotation: NdeI
sequence: https://synbiohub.org/public/igem/BBa_K1592014_sequence/1

GS Linker
type: SequenceAnnotation
persistent identity: annotation2456671
display id: annotation2456671
version: 1
top level: Si-tag1+2+
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1592014/annotation2456671/range2456671/1

https://synbiohub.org/public/igem/BBa_K1592014/annotation2456671/range2456671/1
type: Range
persistent identity: range2456671
display id: range2456671
version: 1
direction: direction/forward
top level: Si-tag1+2+
start: 934
end: 978
orientation: inline

Si-tag 123
type: SequenceAnnotation
persistent identity: annotation2456669
display id: annotation2456669
version: 1
top level: Si-tag1+2+
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1592014/annotation2456669/range2456669/1

https://synbiohub.org/public/igem/BBa_K1592014/annotation2456669/range2456669/1
type: Range
persistent identity: range2456669
display id: range2456669
version: 1
direction: direction/forward
top level: Si-tag1+2+
start: 106
end: 927
orientation: inline

YLcwp3
type: SequenceAnnotation
persistent identity: annotation2456673
display id: annotation2456673
version: 1
top level: Si-tag1+2+
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1592014/annotation2456673/range2456673/1

https://synbiohub.org/public/igem/BBa_K1592014/annotation2456673/range2456673/1
type: Range
persistent identity: range2456673
display id: range2456673
version: 1
direction: direction/forward
top level: Si-tag1+2+
start: 985
end: 1350
orientation: inline

SalI
type: SequenceAnnotation
persistent identity: annotation2456670
display id: annotation2456670
version: 1
top level: Si-tag1+2+
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1592014/annotation2456670/range2456670/1

https://synbiohub.org/public/igem/BBa_K1592014/annotation2456670/range2456670/1
type: Range
persistent identity: range2456670
display id: range2456670
version: 1
direction: direction/forward
top level: Si-tag1+2+
start: 928
end: 933
orientation: inline

LIP2 prepro
type: SequenceAnnotation
persistent identity: annotation2456667
display id: annotation2456667
version: 1
top level: Si-tag1+2+
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1592014/annotation2456667/range2456667/1

https://synbiohub.org/public/igem/BBa_K1592014/annotation2456667/range2456667/1
type: Range
persistent identity: range2456667
display id: range2456667
version: 1
direction: direction/forward
top level: Si-tag1+2+
start: 1
end: 99
orientation: inline

BamHI
type: SequenceAnnotation
persistent identity: annotation2456668
display id: annotation2456668
version: 1
top level: Si-tag1+2+
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1592014/annotation2456668/range2456668/1

https://synbiohub.org/public/igem/BBa_K1592014/annotation2456668/range2456668/1
type: Range
persistent identity: range2456668
display id: range2456668
version: 1
direction: direction/forward
top level: Si-tag1+2+
start: 100
end: 105
orientation: inline

NdeI
type: SequenceAnnotation
persistent identity: annotation2456672
display id: annotation2456672
version: 1
top level: Si-tag1+2+
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1592014/annotation2456672/range2456672/1

https://synbiohub.org/public/igem/BBa_K1592014/annotation2456672/range2456672/1
type: Range
persistent identity: range2456672
display id: range2456672
version: 1
direction: direction/forward
top level: Si-tag1+2+
start: 979
end: 984
orientation: inline

BBa_K1614002
type: ComponentDefinition
persistent identity: BBa K1614002
display id: BBa_K1614002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: transcription cassette of BBF RFC 110
creator: Stefan Holderbach
created: 2015-09-13T11:00:00Z
modified: 2015-09-16T05:48:58Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2031_
m user id: 24664
owner id: 12380
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Unit for RFC 110 assembly, clonable in RFC 10 vectors like pSB1C3.
mutable notes: T7 promoter for expression
mutable provenance: Standard assembly construct for RFC 110 assembly
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence: https://synbiohub.org/public/igem/BBa_K1614002_sequence/1

BBa_K1659301
type: ComponentDefinition
persistent identity: BBa K1659301
display id: BBa_K1659301
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Micrococcal Nuclease (DNase) fused at N-terminal with DsbA signal sequence
creator: Wei Chung Kong
created: 2015-08-27T11:00:00Z
modified: 2015-09-14T08:14:39Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2077_
m user id: 24524
owner id: 24524
owning group id: 9
status: status/Planning
bookmark: false
mutable description: to be updated
mutable notes: to be updated
mutable provenance: to be updated
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: start
sequence annotation: Hisx6
sequence annotation: Micrococcal DNase
sequence annotation: DsbA 2-19 signal sequence
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_K1659301_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation2449089
display id: annotation2449089
version: 1
top level: BBa_K1659301
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1659301/annotation2449089/range2449089/1

https://synbiohub.org/public/igem/BBa_K1659301/annotation2449089/range2449089/1
type: Range
persistent identity: range2449089
display id: range2449089
version: 1
direction: direction/forward
top level: BBa_K1659301
start: 1
end: 3
orientation: inline

Hisx6
type: SequenceAnnotation
persistent identity: annotation2449092
display id: annotation2449092
version: 1
top level: BBa_K1659301
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1659301/annotation2449092/range2449092/1

https://synbiohub.org/public/igem/BBa_K1659301/annotation2449092/range2449092/1
type: Range
persistent identity: range2449092
display id: range2449092
version: 1
direction: direction/forward
top level: BBa_K1659301
start: 559
end: 576
orientation: inline

Micrococcal DNase
type: SequenceAnnotation
persistent identity: annotation2449091
display id: annotation2449091
version: 1
top level: BBa_K1659301
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1659301/annotation2449091/range2449091/1

https://synbiohub.org/public/igem/BBa_K1659301/annotation2449091/range2449091/1
type: Range
persistent identity: range2449091
display id: range2449091
version: 1
direction: direction/forward
top level: BBa_K1659301
start: 58
end: 558
orientation: inline

DsbA 2-19 signal sequence
type: SequenceAnnotation
persistent identity: annotation2449090
display id: annotation2449090
version: 1
top level: BBa_K1659301
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1659301/annotation2449090/range2449090/1

https://synbiohub.org/public/igem/BBa_K1659301/annotation2449090/range2449090/1
type: Range
persistent identity: range2449090
display id: range2449090
version: 1
direction: direction/forward
top level: BBa_K1659301
start: 4
end: 57
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2449093
display id: annotation2449093
version: 1
top level: BBa_K1659301
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1659301/annotation2449093/range2449093/1

https://synbiohub.org/public/igem/BBa_K1659301/annotation2449093/range2449093/1
type: Range
persistent identity: range2449093
display id: range2449093
version: 1
direction: direction/forward
top level: BBa_K1659301
start: 577
end: 582
orientation: inline

BBa_K1602058
type: ComponentDefinition
persistent identity: BBa K1602058
display id: BBa_K1602058
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0034-yagE-B0034-yjhG-B0034-yqhD
creator: Leon Kraus, Jonathan Pletzer-Zelgert, Jonas Sindlinger, Carmen Sakas Gandullo, Sebastian Holzner, Elena Tonchevska, Mirjam Haun
created: 2015-09-15T11:00:00Z
modified: 2015-09-18T06:37:07Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2019_
m user id: 26220
owner id: 25990
owning group id: 9
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1602002
component: BBa_K1602013
component: BBa_B0034
component: BBa_K1602014
sequence annotation: BBa_K1602013
sequence annotation: BBa_K1602014
sequence annotation: BBa_K1602002
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K1602058_sequence/1

BBa_K1602002
type: Component
persistent identity: component2463128
display id: component2463128
version: 1
top level: BBa_K1602058
access: public
definition: https://synbiohub.org/public/igem/BBa_K1602002/1

BBa_K1602013
type: Component
persistent identity: component2463131
display id: component2463131
version: 1
top level: BBa_K1602058
access: public
definition: BBa_K1602013

BBa_B0034
type: Component
persistent identity: component2463129
display id: component2463129
version: 1
top level: BBa_K1602058
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1602014
type: Component
persistent identity: component2463124
display id: component2463124
version: 1
top level: BBa_K1602058
access: public
definition: BBa_K1602014

BBa_K1602013
type: SequenceAnnotation
persistent identity: annotation2463131
display id: annotation2463131
version: 1
top level: BBa_K1602058
location: https://synbiohub.org/public/igem/BBa_K1602058/annotation2463131/range2463131/1
component: BBa_K1602013

https://synbiohub.org/public/igem/BBa_K1602058/annotation2463131/range2463131/1
type: Range
persistent identity: range2463131
display id: range2463131
version: 1
direction: direction/reverse
top level: BBa_K1602058
start: 2948
end: 4111
orientation: inline

BBa_K1602014
type: SequenceAnnotation
persistent identity: annotation2463124
display id: annotation2463124
version: 1
top level: BBa_K1602058
location: https://synbiohub.org/public/igem/BBa_K1602058/annotation2463124/range2463124/1
component: BBa_K1602014

https://synbiohub.org/public/igem/BBa_K1602058/annotation2463124/range2463124/1
type: Range
persistent identity: range2463124
display id: range2463124
version: 1
direction: direction/reverse
top level: BBa_K1602058
start: 1
end: 927
orientation: inline

BBa_K1602002
type: SequenceAnnotation
persistent identity: annotation2463128
display id: annotation2463128
version: 1
top level: BBa_K1602058
location: https://synbiohub.org/public/igem/BBa_K1602058/annotation2463128/range2463128/1
component: BBa_K1602002

https://synbiohub.org/public/igem/BBa_K1602058/annotation2463128/range2463128/1
type: Range
persistent identity: range2463128
display id: range2463128
version: 1
direction: direction/reverse
top level: BBa_K1602058
start: 936
end: 2921
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2463129
display id: annotation2463129
version: 1
top level: BBa_K1602058
location: https://synbiohub.org/public/igem/BBa_K1602058/annotation2463129/range2463129/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1602058/annotation2463129/range2463129/1
type: Range
persistent identity: range2463129
display id: range2463129
version: 1
direction: direction/reverse
top level: BBa_K1602058
start: 2930
end: 2941
orientation: inline

BBa_K1758360
type: ComponentDefinition
persistent identity: BBa K1758360
display id: BBa_K1758360
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: gabR gene and gabT promoter from Bacillus subtilis
creator: Team Bielefeld-CeBiTec 2015
created: 2015-08-21T11:00:00Z
modified: 2015-09-18T04:39:39Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2180_
m user id: 27935
owner id: 26587
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This is a part of the gab operon of Bacillus subtilis. It includes the gene for the repressor protein GabR under the control of its natural promoter as well as the gabT promoter, which is regulated by GabR. GabR is inactivated by γ-aminobutyrate(GABA). The expression of any gene that is inserted downstream of this part can therefore be induced by GABA.
mutable notes: The coding sequence of gabR is codon optimized for expression in E. coli.
mutable provenance: This part was obtained by gene synthesis.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: gabR
sequence: https://synbiohub.org/public/igem/BBa_K1758360_sequence/1

gabR
type: SequenceAnnotation
persistent identity: annotation2436848
display id: annotation2436848
version: 1
top level: BBa_K1758360
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1758360/annotation2436848/range2436848/1

https://synbiohub.org/public/igem/BBa_K1758360/annotation2436848/range2436848/1
type: Range
persistent identity: range2436848
display id: range2436848
version: 1
direction: direction/reverse
top level: BBa_K1758360
start: 1
end: 1440
orientation: inline

BBa_K1583112
type: ComponentDefinition
persistent identity: BBa K1583112
display id: BBa_K1583112
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pRha + CsgA & GFP in same operon
creator: Stefan Robert Marsden, Max van 't Hof, Samantha Basalo Vazquez
created: 2015-09-14T11:00:00Z
modified: 2015-11-13T09:41:52Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2000_
m user id: 24465
owner id: 24463
owning group id: 9
status: status/Planning
bookmark: true
mutable description: CsgA is a protein monomer which can aggregate to form amyloid nanowires in natural biofilms taken from E.coli K-12 MG1655. The aggregation to the nanowire ('curli') is induced by the membrane protein CsgB. CsgC, CsgE, CsgF and CsgG act as chaperones for CsgA during translation and export to the extracellular space. This part was designed to measure intracellular expression rates of CsgA coupled to fluorescence (GFP) by cloning the biobrick I13504 into the same operon which is under control of the Rhamnose promoter.
mutable notes: The scar is a stop codon, but no terminator. Like this, cloning a GFP which contains an RBS we intended to directly couple CsgA expression to expression of GFP.
mutable provenance: This part was synthesized. The rhamnose promoter was used by the iGEM 2014 TU Delft team and originally added by the iGEM12 Paris Bettencourt team. RBS and CsgA originate from E. coli K-12 MG1655
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_I13504
component: BBa_K1583100
sequence annotation: BBa_K1583100
sequence annotation: BBa_I13504
sequence: https://synbiohub.org/public/igem/BBa_K1583112_sequence/1

BBa_I13504
type: Component
persistent identity: component2457335
display id: component2457335
version: 1
top level: BBa_K1583112
access: public
definition: https://synbiohub.org/public/igem/BBa_I13504/1

BBa_K1583100
type: Component
persistent identity: component2457325
display id: component2457325
version: 1
top level: BBa_K1583112
access: public
definition: BBa_K1583100

BBa_K1583100
type: SequenceAnnotation
persistent identity: annotation2457325
display id: annotation2457325
version: 1
top level: BBa_K1583112
location: https://synbiohub.org/public/igem/BBa_K1583112/annotation2457325/range2457325/1
component: BBa_K1583100

https://synbiohub.org/public/igem/BBa_K1583112/annotation2457325/range2457325/1
type: Range
persistent identity: range2457325
display id: range2457325
version: 1
direction: direction/reverse
top level: BBa_K1583112
start: 1
end: 638
orientation: inline

BBa_I13504
type: SequenceAnnotation
persistent identity: annotation2457335
display id: annotation2457335
version: 1
top level: BBa_K1583112
location: https://synbiohub.org/public/igem/BBa_K1583112/annotation2457335/range2457335/1
component: BBa_I13504

https://synbiohub.org/public/igem/BBa_K1583112/annotation2457335/range2457335/1
type: Range
persistent identity: range2457335
display id: range2457335
version: 1
direction: direction/reverse
top level: BBa_K1583112
start: 647
end: 1521
orientation: inline

BBa_K1763001
type: ComponentDefinition
persistent identity: BBa K1763001
display id: BBa_K1763001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Apis mellifera (honeybee) silk fibroin 3 with regulatory elements
creator: Daniel Cancilla
created: 2015-05-20T11:00:00Z
modified: 2015-05-22T01:05:28Z
discontinued: false
dominant: false
group u list: _2185_
m user id: 18035
owner id: 18035
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Same as part BBa_K1763000 with regulatory elements upstream. These include lac promoter and rbs (will update later)
mutable notes: Will update later
mutable provenance: Apis mellifera genome. Reference Genbank FJ235090.1
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: BBa_R0010
sequence annotation: conserved
sequence annotation: Honey bee silk coding region
sequence annotation: start codon
sequence annotation: BBa_K1763000
sequence annotation: BBa_B0034
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_K1763001_sequence/1

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2432167
display id: annotation2432167
version: 1
top level: BBa_K1763001
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1763001/annotation2432167/range2432167/1

https://synbiohub.org/public/igem/BBa_K1763001/annotation2432167/range2432167/1
type: Range
persistent identity: range2432167
display id: range2432167
version: 1
direction: direction/forward
top level: BBa_K1763001
start: 1
end: 200
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation2432170
display id: annotation2432170
version: 1
top level: BBa_K1763001
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K1763001/annotation2432170/range2432170/1

https://synbiohub.org/public/igem/BBa_K1763001/annotation2432170/range2432170/1
type: Range
persistent identity: range2432170
display id: range2432170
version: 1
direction: direction/forward
top level: BBa_K1763001
start: 205
end: 208
orientation: inline

Honey bee silk coding region
type: SequenceAnnotation
persistent identity: annotation2432172
display id: annotation2432172
version: 1
top level: BBa_K1763001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1763001/annotation2432172/range2432172/1

https://synbiohub.org/public/igem/BBa_K1763001/annotation2432172/range2432172/1
type: Range
persistent identity: range2432172
display id: range2432172
version: 1
direction: direction/forward
top level: BBa_K1763001
start: 213
end: 1163
orientation: inline

start codon
type: SequenceAnnotation
persistent identity: annotation2432171
display id: annotation2432171
version: 1
top level: BBa_K1763001
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1763001/annotation2432171/range2432171/1

https://synbiohub.org/public/igem/BBa_K1763001/annotation2432171/range2432171/1
type: Range
persistent identity: range2432171
display id: range2432171
version: 1
direction: direction/forward
top level: BBa_K1763001
start: 213
end: 215
orientation: inline

BBa_K1763000
type: SequenceAnnotation
persistent identity: annotation2432173
display id: annotation2432173
version: 1
top level: BBa_K1763001
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1763001/annotation2432173/range2432173/1

https://synbiohub.org/public/igem/BBa_K1763001/annotation2432173/range2432173/1
type: Range
persistent identity: range2432173
display id: range2432173
version: 1
direction: direction/reverse
top level: BBa_K1763001
start: 213
end: 1163
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2432169
display id: annotation2432169
version: 1
top level: BBa_K1763001
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1763001/annotation2432169/range2432169/1

https://synbiohub.org/public/igem/BBa_K1763001/annotation2432169/range2432169/1
type: Range
persistent identity: range2432169
display id: range2432169
version: 1
direction: direction/reverse
top level: BBa_K1763001
start: 201
end: 212
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2432168
display id: annotation2432168
version: 1
top level: BBa_K1763001
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1763001/annotation2432168/range2432168/1

https://synbiohub.org/public/igem/BBa_K1763001/annotation2432168/range2432168/1
type: Range
persistent identity: range2432168
display id: range2432168
version: 1
direction: direction/forward
top level: BBa_K1763001
start: 173
end: 173
orientation: inline

BBa_K1789002
type: ComponentDefinition
persistent identity: BBa K1789002
display id: BBa_K1789002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS30
creator: Xinyuan Qiu
created: 2015-09-13T11:00:00Z
modified: 2015-09-14T06:03:14Z
discontinued: false
dominant: false
group u list: _2214_
m user id: 21134
owner id: 21134
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This part is another copy of the RBS30 (BBa_B0030). It is a Strong RBS based on Ron Weiss thesis.
mutable notes: The reason why we built this part by ourselves is that the copy of B0030 in the 2015 DNA distribution we received is sequenced as incorrect. And same problem also occurred in several other teams.
mutable provenance: The part sequence was gained from the registry, the part is annealed from the ssDNA synthesized by Life(R).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS30
sequence: https://synbiohub.org/public/igem/BBa_K1789002_sequence/1

RBS30
type: SequenceAnnotation
persistent identity: annotation2454064
display id: annotation2454064
version: 1
top level: BBa_K1789002
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1789002/annotation2454064/range2454064/1

https://synbiohub.org/public/igem/BBa_K1789002/annotation2454064/range2454064/1
type: Range
persistent identity: range2454064
display id: range2454064
version: 1
direction: direction/forward
top level: BBa_K1789002
start: 1
end: 15
orientation: inline

BBa_K1582029
type: ComponentDefinition
persistent identity: BBa K1582029
display id: BBa_K1582029
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Protein Extraction Tag: Linker+sJanus
creator: Dongqi Bao
created: 2015-09-04T11:00:00Z
modified: 2015-09-09T12:05:29Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1998_
m user id: 23270
owner id: 23270
owning group id: 9
status: status/Planning
bookmark: false
mutable description: HFB1+Linker
mutable notes: HFB1+Linker
mutable provenance: HFB1+Linker
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: Linker
sequence annotation: BBa_K1582001
sequence annotation: TEV protease cleavage site
sequence: https://synbiohub.org/public/igem/BBa_K1582029_sequence/1

Linker
type: SequenceAnnotation
persistent identity: annotation2447549
display id: annotation2447549
version: 1
top level: BBa_K1582029
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1582029/annotation2447549/range2447549/1

https://synbiohub.org/public/igem/BBa_K1582029/annotation2447549/range2447549/1
type: Range
persistent identity: range2447549
display id: range2447549
version: 1
direction: direction/forward
top level: BBa_K1582029
start: 1
end: 27
orientation: inline

BBa_K1582001
type: SequenceAnnotation
persistent identity: annotation2444239
display id: annotation2444239
version: 1
top level: BBa_K1582029
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1582029/annotation2444239/range2444239/1

https://synbiohub.org/public/igem/BBa_K1582029/annotation2444239/range2444239/1
type: Range
persistent identity: range2444239
display id: range2444239
version: 1
direction: direction/forward
top level: BBa_K1582029
start: 28
end: 255
orientation: inline

TEV protease cleavage site
type: SequenceAnnotation
persistent identity: annotation2447550
display id: annotation2447550
version: 1
top level: BBa_K1582029
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1582029/annotation2447550/range2447550/1

https://synbiohub.org/public/igem/BBa_K1582029/annotation2447550/range2447550/1
type: Range
persistent identity: range2447550
display id: range2447550
version: 1
direction: direction/forward
top level: BBa_K1582029
start: 1
end: 21
orientation: inline

eYFP_SH3li
type: ComponentDefinition
persistent identity: BBa K1602038
display id: BBa_K1602038
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: eYFP with C-terminal SH3 ligand and RBS BBa_B0034
creator: Jasmin Weber, Michelle Zoeller, Marcus Geissler, Stefan Bohn, Simon Linder, Sebastian Barthel
created: 2015-09-16T11:00:00Z
modified: 2015-09-17T05:11:27Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2019_
m user id: 22022
owner id: 22022
owning group id: 9
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K1602038_sequence/1

BBa_K1692033
type: ComponentDefinition
persistent identity: BBa K1692033
display id: BBa_K1692033
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RFP gDNA
creator: Kirsten Thompson
created: 2015-09-16T11:00:00Z
modified: 2015-09-18T08:22:08Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2110_
m user id: 26427
owner id: 26511
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This gene provides the intermediate for synthesizing gRNA targeting RFP (BBa_J04450). When used in conjuction with the CRISPR/Cas system, this gRNA will bind to and digest the RFP plasmid.
mutable notes: N/A
mutable provenance: Synthesized by Integrated DNA Technologies (IDT). We amplified the sequence, digested with EcoRI and SpeI and ligated into the PSB1C3 vector.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
sequence: https://synbiohub.org/public/igem/BBa_K1692033_sequence/1

BBa_K1640020
type: ComponentDefinition
persistent identity: BBa K1640020
display id: BBa_K1640020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: psbA
creator: Meghan Cook
created: 2015-09-09T11:00:00Z
modified: 2015-09-18T08:54:57Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2057_
m user id: 28565
owner id: 28565
owning group id: 9
status: status/Planning
bookmark: true
mutable description: n/a
mutable notes: n/a
mutable provenance: n/a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: double terminator
sequence annotation: psbA
sequence annotation: rbs
sequence: https://synbiohub.org/public/igem/BBa_K1640020_sequence/1

double terminator
type: SequenceAnnotation
persistent identity: annotation2448253
display id: annotation2448253
version: 1
top level: BBa_K1640020
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1640020/annotation2448253/range2448253/1

https://synbiohub.org/public/igem/BBa_K1640020/annotation2448253/range2448253/1
type: Range
persistent identity: range2448253
display id: range2448253
version: 1
direction: direction/forward
top level: BBa_K1640020
start: 1075
end: 1203
orientation: inline

psbA
type: SequenceAnnotation
persistent identity: annotation2448252
display id: annotation2448252
version: 1
top level: BBa_K1640020
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1640020/annotation2448252/range2448252/1

https://synbiohub.org/public/igem/BBa_K1640020/annotation2448252/range2448252/1
type: Range
persistent identity: range2448252
display id: range2448252
version: 1
direction: direction/forward
top level: BBa_K1640020
start: 16
end: 1074
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2448251
display id: annotation2448251
version: 1
top level: BBa_K1640020
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1640020/annotation2448251/range2448251/1

https://synbiohub.org/public/igem/BBa_K1640020/annotation2448251/range2448251/1
type: Range
persistent identity: range2448251
display id: range2448251
version: 1
direction: direction/forward
top level: BBa_K1640020
start: 1
end: 15
orientation: inline

BBa_K1632003
type: ComponentDefinition
persistent identity: BBa K1632003
display id: BBa_K1632003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: fim switch[default OFF](Tokyo_Tech/J23119)_rbs_gfp
creator: Riku Shinohara
created: 2015-08-12T11:00:00Z
modified: 2015-09-15T06:52:06Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _2049_
m user id: 24760
owner id: 22687
owning group id: 9
status: status/Planning
bookmark: false
mutable description: 1
mutable notes: 1
mutable provenance: 1
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_E0040
component: BBa_K1632001
component: BBa_B0034
sequence annotation: BBa_E0040
sequence annotation: BBa_K1632001
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K1632003_sequence/1

BBa_E0040
type: Component
persistent identity: component2435339
display id: component2435339
version: 1
top level: BBa_K1632003
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_K1632001
type: Component
persistent identity: component2435335
display id: component2435335
version: 1
top level: BBa_K1632003
access: public
definition: BBa_K1632001

BBa_B0034
type: Component
persistent identity: component2435337
display id: component2435337
version: 1
top level: BBa_K1632003
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2435339
display id: annotation2435339
version: 1
top level: BBa_K1632003
location: https://synbiohub.org/public/igem/BBa_K1632003/annotation2435339/range2435339/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K1632003/annotation2435339/range2435339/1
type: Range
persistent identity: range2435339
display id: range2435339
version: 1
direction: direction/reverse
top level: BBa_K1632003
start: 459
end: 1178
orientation: inline

BBa_K1632001
type: SequenceAnnotation
persistent identity: annotation2435335
display id: annotation2435335
version: 1
top level: BBa_K1632003
location: https://synbiohub.org/public/igem/BBa_K1632003/annotation2435335/range2435335/1
component: BBa_K1632001

https://synbiohub.org/public/igem/BBa_K1632003/annotation2435335/range2435335/1
type: Range
persistent identity: range2435335
display id: range2435335
version: 1
direction: direction/reverse
top level: BBa_K1632003
start: 1
end: 432
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2435337
display id: annotation2435337
version: 1
top level: BBa_K1632003
location: https://synbiohub.org/public/igem/BBa_K1632003/annotation2435337/range2435337/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1632003/annotation2435337/range2435337/1
type: Range
persistent identity: range2435337
display id: range2435337
version: 1
direction: direction/reverse
top level: BBa_K1632003
start: 441
end: 452
orientation: inline

DspB
type: ComponentDefinition
persistent identity: BBa K1659200
display id: BBa_K1659200
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Dispersin B, an antibiofilm enzyme that specifically hydrolyses PGA polymers
creator: Wei Chung Kong
created: 2015-08-27T11:00:00Z
modified: 2015-09-12T03:59:05Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2077_
m user id: 24524
owner id: 24524
owning group id: 9
status: status/Planning
bookmark: false
mutable description: to be updated
mutable notes: to be updated
mutable provenance: to be updated
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: start
sequence annotation: stop
sequence annotation: Hisx6
sequence annotation: DspB
sequence: https://synbiohub.org/public/igem/BBa_K1659200_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation2449109
display id: annotation2449109
version: 1
top level: DspB
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1659200/annotation2449109/range2449109/1

https://synbiohub.org/public/igem/BBa_K1659200/annotation2449109/range2449109/1
type: Range
persistent identity: range2449109
display id: range2449109
version: 1
direction: direction/forward
top level: DspB
start: 1
end: 3
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2449112
display id: annotation2449112
version: 1
top level: DspB
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1659200/annotation2449112/range2449112/1

https://synbiohub.org/public/igem/BBa_K1659200/annotation2449112/range2449112/1
type: Range
persistent identity: range2449112
display id: range2449112
version: 1
direction: direction/forward
top level: DspB
start: 1105
end: 1110
orientation: inline

Hisx6
type: SequenceAnnotation
persistent identity: annotation2449111
display id: annotation2449111
version: 1
top level: DspB
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1659200/annotation2449111/range2449111/1

https://synbiohub.org/public/igem/BBa_K1659200/annotation2449111/range2449111/1
type: Range
persistent identity: range2449111
display id: range2449111
version: 1
direction: direction/forward
top level: DspB
start: 1087
end: 1104
orientation: inline

DspB
type: SequenceAnnotation
persistent identity: annotation2449110
display id: annotation2449110
version: 1
top level: DspB
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1659200/annotation2449110/range2449110/1

https://synbiohub.org/public/igem/BBa_K1659200/annotation2449110/range2449110/1
type: Range
persistent identity: range2449110
display id: range2449110
version: 1
direction: direction/forward
top level: DspB
start: 4
end: 1086
orientation: inline

BBa_K1583113
type: ComponentDefinition
persistent identity: BBa K1583113
display id: BBa_K1583113
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pAra + fusion protein BFP_Spycatcher_His
creator: Max van
created: 2015-09-13T11:00:00Z
modified: 2015-09-17T05:21:01Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _2000_
m user id: 24478
owner id: 24478
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Inducible arabinose promoter controlling expression of BFP_SpyCatcher_His.
mutable notes: The start condon of SpyCatcher and the stop codon of BFP needed to be removed to ensure that one complete protein was expressed.
mutable provenance: These part was synthesized as linear DNA and only restriction/ligation was done to insert this into the backbone.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1583061
component: BBa_K1583054
component: BBa_K1583017
component: BBa_K1583018
component: BBa_K206000
component: BBa_G0002
sequence annotation: BBa_G0002
sequence annotation: BBa_K1583061
sequence annotation: BBa_K1583017
sequence annotation: BBa_K1583054
sequence annotation: BBa_K1583018
sequence annotation: BBa_K206000
sequence: https://synbiohub.org/public/igem/BBa_K1583113_sequence/1

BBa_K1583061
type: Component
persistent identity: component2454217
display id: component2454217
version: 1
top level: BBa_K1583113
access: public
definition: https://synbiohub.org/public/igem/BBa_K1583061/1

BBa_K1583054
type: Component
persistent identity: component2454211
display id: component2454211
version: 1
top level: BBa_K1583113
access: public
definition: https://synbiohub.org/public/igem/BBa_K1583054/1

BBa_K1583017
type: Component
persistent identity: component2454218
display id: component2454218
version: 1
top level: BBa_K1583113
access: public
definition: https://synbiohub.org/public/igem/BBa_K1583017/1

BBa_K1583018
type: Component
persistent identity: component2454222
display id: component2454222
version: 1
top level: BBa_K1583113
access: public
definition: https://synbiohub.org/public/igem/BBa_K1583018/1

BBa_K206000
type: Component
persistent identity: component2454215
display id: component2454215
version: 1
top level: BBa_K1583113
access: public
definition: https://synbiohub.org/public/igem/BBa_K206000/1

BBa_G0002
type: Component
persistent identity: component2454216
display id: component2454216
version: 1
top level: BBa_K1583113
access: public
definition: https://synbiohub.org/public/igem/BBa_G0002/1

BBa_G0002
type: SequenceAnnotation
persistent identity: annotation2454216
display id: annotation2454216
version: 1
top level: BBa_K1583113
location: https://synbiohub.org/public/igem/BBa_K1583113/annotation2454216/range2454216/1
component: BBa_G0002

https://synbiohub.org/public/igem/BBa_K1583113/annotation2454216/range2454216/1
type: Range
persistent identity: range2454216
display id: range2454216
version: 1
direction: direction/reverse
top level: BBa_K1583113
start: 137
end: 144
orientation: inline

BBa_K1583061
type: SequenceAnnotation
persistent identity: annotation2454217
display id: annotation2454217
version: 1
top level: BBa_K1583113
location: https://synbiohub.org/public/igem/BBa_K1583113/annotation2454217/range2454217/1
component: BBa_K1583061

https://synbiohub.org/public/igem/BBa_K1583113/annotation2454217/range2454217/1
type: Range
persistent identity: range2454217
display id: range2454217
version: 1
direction: direction/reverse
top level: BBa_K1583113
start: 147
end: 158
orientation: inline

BBa_K1583017
type: SequenceAnnotation
persistent identity: annotation2454218
display id: annotation2454218
version: 1
top level: BBa_K1583113
location: https://synbiohub.org/public/igem/BBa_K1583113/annotation2454218/range2454218/1
component: BBa_K1583017

https://synbiohub.org/public/igem/BBa_K1583113/annotation2454218/range2454218/1
type: Range
persistent identity: range2454218
display id: range2454218
version: 1
direction: direction/reverse
top level: BBa_K1583113
start: 162
end: 860
orientation: inline

BBa_K1583054
type: SequenceAnnotation
persistent identity: annotation2454211
display id: annotation2454211
version: 1
top level: BBa_K1583113
location: https://synbiohub.org/public/igem/BBa_K1583113/annotation2454211/range2454211/1
component: BBa_K1583054

https://synbiohub.org/public/igem/BBa_K1583113/annotation2454211/range2454211/1
type: Range
persistent identity: range2454211
display id: range2454211
version: 1
direction: direction/reverse
top level: BBa_K1583113
start: 1
end: 6
orientation: inline

BBa_K1583018
type: SequenceAnnotation
persistent identity: annotation2454222
display id: annotation2454222
version: 1
top level: BBa_K1583113
location: https://synbiohub.org/public/igem/BBa_K1583113/annotation2454222/range2454222/1
component: BBa_K1583018

https://synbiohub.org/public/igem/BBa_K1583113/annotation2454222/range2454222/1
type: Range
persistent identity: range2454222
display id: range2454222
version: 1
direction: direction/reverse
top level: BBa_K1583113
start: 861
end: 1268
orientation: inline

BBa_K206000
type: SequenceAnnotation
persistent identity: annotation2454215
display id: annotation2454215
version: 1
top level: BBa_K1583113
location: https://synbiohub.org/public/igem/BBa_K1583113/annotation2454215/range2454215/1
component: BBa_K206000

https://synbiohub.org/public/igem/BBa_K1583113/annotation2454215/range2454215/1
type: Range
persistent identity: range2454215
display id: range2454215
version: 1
direction: direction/reverse
top level: BBa_K1583113
start: 7
end: 136
orientation: inline

BBa_K1696003
type: ComponentDefinition
persistent identity: BBa K1696003
display id: BBa_K1696003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: L-lactate producing part1
creator: Yue Liu
created: 2015-09-10T11:00:00Z
modified: 2015-09-11T11:49:17Z
discontinued: false
dominant: false
group u list: _2114_
m user id: 16881
owner id: 16881
owning group id: 9
status: status/Planning
bookmark: false
mutable description: We choose a strong promoter+ strong RBS to construct L-Lactate part. The NAD+-dependent L-lactate dehydrogenase gene is from Lactobacillus casei.
mutable notes: Strong promoter+strong RBS
mutable provenance: It's our design based on BBa_K1696001.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: ldhE-Lc
sequence annotation: J23100
sequence annotation: B0034
sequence annotation: B0014
sequence: https://synbiohub.org/public/igem/BBa_K1696003_sequence/1

ldhE-Lc
type: SequenceAnnotation
persistent identity: annotation2449375
display id: annotation2449375
version: 1
top level: BBa_K1696003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1696003/annotation2449375/range2449375/1

https://synbiohub.org/public/igem/BBa_K1696003/annotation2449375/range2449375/1
type: Range
persistent identity: range2449375
display id: range2449375
version: 1
direction: direction/forward
top level: BBa_K1696003
start: 69
end: 1049
orientation: inline

J23100
type: SequenceAnnotation
persistent identity: annotation2449370
display id: annotation2449370
version: 1
top level: BBa_K1696003
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1696003/annotation2449370/range2449370/1

https://synbiohub.org/public/igem/BBa_K1696003/annotation2449370/range2449370/1
type: Range
persistent identity: range2449370
display id: range2449370
version: 1
direction: direction/forward
top level: BBa_K1696003
start: 1
end: 35
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2449374
display id: annotation2449374
version: 1
top level: BBa_K1696003
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1696003/annotation2449374/range2449374/1

https://synbiohub.org/public/igem/BBa_K1696003/annotation2449374/range2449374/1
type: Range
persistent identity: range2449374
display id: range2449374
version: 1
direction: direction/forward
top level: BBa_K1696003
start: 51
end: 62
orientation: inline

B0014
type: SequenceAnnotation
persistent identity: annotation2449376
display id: annotation2449376
version: 1
top level: BBa_K1696003
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1696003/annotation2449376/range2449376/1

https://synbiohub.org/public/igem/BBa_K1696003/annotation2449376/range2449376/1
type: Range
persistent identity: range2449376
display id: range2449376
version: 1
direction: direction/forward
top level: BBa_K1696003
start: 1064
end: 1158
orientation: inline

BBa_K1795004
type: ComponentDefinition
persistent identity: BBa K1795004
display id: BBa_K1795004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: R0062 gRNA under R0040
creator: Andrew D Halleran, Joseph L Maniaci
created: 2015-09-16T11:00:00Z
modified: 2015-09-17T09:41:10Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2220_
m user id: 27446
owner id: 27446
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This sgRNA sequence was designed to target the Promoter Bba_R0062 with the N20 sequence tacaggtttacgcaagaaaa . If this gRNA is expressed in a cell with a form of the dCas9 protein, the protein will be targeted to the N20 sequence and repress gene expression. The part is under the control of the promoter Bba_R0040. Promoter Bba_R0040 was chosen because it does not contain a PAM and therefore cannot be inadvertently repressed by dCas9 due to unintentional homology in the N20 sequence. Expression of this part can be repressed if TetR is expressed in the cell without Tetracycline present. If TetR and Tetracycline (or its analog aTc) are both present, the part will be expressed.
mutable notes: We made sure to use a different promoter for this part than the one which the part targets.
mutable provenance: The basic template for the sgRNA sequence was taken from the supplementary information of Qi LS, Larson MH, Gilbert LA, et al. Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression. Cell. 2013;152(5):1173-1183. doi:10.1016/j.cell.2013.02.022.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1795104
component: BBa_K1795100
component: BBa_R0040
component: BBa_B0015
sequence annotation: BBa_K1795104
sequence annotation: BBa_R0040
sequence annotation: BBa_K1795100
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K1795004_sequence/1

BBa_K1795104
type: Component
persistent identity: component2467727
display id: component2467727
version: 1
top level: BBa_K1795004
access: public
definition: https://synbiohub.org/public/igem/BBa_K1795104/1

BBa_K1795100
type: Component
persistent identity: component2467728
display id: component2467728
version: 1
top level: BBa_K1795004
access: public
definition: https://synbiohub.org/public/igem/BBa_K1795100/1

BBa_R0040
type: Component
persistent identity: component2467722
display id: component2467722
version: 1
top level: BBa_K1795004
access: public
definition: p(tetR)

BBa_B0015
type: Component
persistent identity: component2467735
display id: component2467735
version: 1
top level: BBa_K1795004
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_K1795104
type: SequenceAnnotation
persistent identity: annotation2467727
display id: annotation2467727
version: 1
top level: BBa_K1795004
location: https://synbiohub.org/public/igem/BBa_K1795004/annotation2467727/range2467727/1
component: BBa_K1795104

https://synbiohub.org/public/igem/BBa_K1795004/annotation2467727/range2467727/1
type: Range
persistent identity: range2467727
display id: range2467727
version: 1
direction: direction/reverse
top level: BBa_K1795004
start: 55
end: 74
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2467722
display id: annotation2467722
version: 1
top level: BBa_K1795004
location: https://synbiohub.org/public/igem/BBa_K1795004/annotation2467722/range2467722/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K1795004/annotation2467722/range2467722/1
type: Range
persistent identity: range2467722
display id: range2467722
version: 1
direction: direction/forward
top level: BBa_K1795004
start: 1
end: 54
orientation: inline

BBa_K1795100
type: SequenceAnnotation
persistent identity: annotation2467728
display id: annotation2467728
version: 1
top level: BBa_K1795004
location: https://synbiohub.org/public/igem/BBa_K1795004/annotation2467728/range2467728/1
component: BBa_K1795100

https://synbiohub.org/public/igem/BBa_K1795004/annotation2467728/range2467728/1
type: Range
persistent identity: range2467728
display id: range2467728
version: 1
direction: direction/reverse
top level: BBa_K1795004
start: 75
end: 156
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2467735
display id: annotation2467735
version: 1
top level: BBa_K1795004
location: https://synbiohub.org/public/igem/BBa_K1795004/annotation2467735/range2467735/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1795004/annotation2467735/range2467735/1
type: Range
persistent identity: range2467735
display id: range2467735
version: 1
direction: direction/reverse
top level: BBa_K1795004
start: 157
end: 285
orientation: inline

xynA-PDZli
type: ComponentDefinition
persistent identity: BBa K1602035
display id: BBa_K1602035
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Inducible generator of RBS-xynA-PDZlig
creator: Jasmin Weber, Michelle Zoeller, Marcus Geissler, Stefan Bohn, Simon Linder, Sebastian Barthel
created: 2015-09-15T11:00:00Z
modified: 2015-09-16T07:45:09Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2019_
m user id: 22022
owner id: 22022
owning group id: 9
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K1602033
component: BBa_I719005
sequence annotation: BBa_I719005
sequence annotation: BBa_K1602033
sequence: https://synbiohub.org/public/igem/BBa_K1602035_sequence/1

BBa_K1602033
type: Component
persistent identity: component2463047
display id: component2463047
version: 1
top level: xynA-PDZli
access: public
definition: https://synbiohub.org/public/igem/BBa_K1602033/1

BBa_I719005
type: Component
persistent identity: component2463046
display id: component2463046
version: 1
top level: xynA-PDZli
access: public
definition: https://synbiohub.org/public/igem/BBa_I719005/1

BBa_I719005
type: SequenceAnnotation
persistent identity: annotation2463046
display id: annotation2463046
version: 1
top level: xynA-PDZli
location: https://synbiohub.org/public/igem/BBa_K1602035/annotation2463046/range2463046/1
component: BBa_I719005

https://synbiohub.org/public/igem/BBa_K1602035/annotation2463046/range2463046/1
type: Range
persistent identity: range2463046
display id: range2463046
version: 1
direction: direction/reverse
top level: xynA-PDZli
start: 1
end: 23
orientation: inline

BBa_K1602033
type: SequenceAnnotation
persistent identity: annotation2463047
display id: annotation2463047
version: 1
top level: xynA-PDZli
location: https://synbiohub.org/public/igem/BBa_K1602035/annotation2463047/range2463047/1
component: BBa_K1602033

https://synbiohub.org/public/igem/BBa_K1602035/annotation2463047/range2463047/1
type: Range
persistent identity: range2463047
display id: range2463047
version: 1
direction: direction/reverse
top level: xynA-PDZli
start: 32
end: 724
orientation: inline

BBa_K1690003
type: ComponentDefinition
persistent identity: BBa K1690003
display id: BBa_K1690003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Mating pheromone (L. elongisporus)
creator: Anna Sosnova, Pavel Zach
created: 2015-09-16T11:00:00Z
modified: 2015-09-17T05:34:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2108_
m user id: 25090
owner id: 25090
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This is a coding sequence of L. elongisporus mating pheromone together with a secretion tag. Expression in the yeast cell causes secretion of the mature mating pheromone into the medium. Cells with a corresponding STE2 receptor react to this pheromone by the activation of the pheromone mating pathway. Detailed description can be obtained from the [http://2015.igem.org/Team:Czech_Republic/Project/Synthetic_signals_and_receptors#Signals iGEM 2015 Czech Republic wiki].
mutable notes: The secretion tag was obtained by a genomic PCR from S. cerevisiae. The pheromone sequence was synthetized.
mutable provenance: This part is derived from [http://parts.igem.org/Part:BBa_K1690000 BBa_K1690000]. Using site-directed mutagenesis, we replaced the original pheromone sequence.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: start
sequence annotation: stop
sequence annotation: Kozak
sequence annotation: Pheromone
sequence annotation: Secretion tag
sequence: https://synbiohub.org/public/igem/BBa_K1690003_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation2464314
display id: annotation2464314
version: 1
top level: BBa_K1690003
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1690003/annotation2464314/range2464314/1

https://synbiohub.org/public/igem/BBa_K1690003/annotation2464314/range2464314/1
type: Range
persistent identity: range2464314
display id: range2464314
version: 1
direction: direction/forward
top level: BBa_K1690003
start: 7
end: 9
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2464323
display id: annotation2464323
version: 1
top level: BBa_K1690003
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1690003/annotation2464323/range2464323/1

https://synbiohub.org/public/igem/BBa_K1690003/annotation2464323/range2464323/1
type: Range
persistent identity: range2464323
display id: range2464323
version: 1
direction: direction/forward
top level: BBa_K1690003
start: 313
end: 315
orientation: inline

Kozak
type: SequenceAnnotation
persistent identity: annotation2464313
display id: annotation2464313
version: 1
top level: BBa_K1690003
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1690003/annotation2464313/range2464313/1

https://synbiohub.org/public/igem/BBa_K1690003/annotation2464313/range2464313/1
type: Range
persistent identity: range2464313
display id: range2464313
version: 1
direction: direction/forward
top level: BBa_K1690003
start: 1
end: 6
orientation: inline

Pheromone
type: SequenceAnnotation
persistent identity: annotation2464322
display id: annotation2464322
version: 1
top level: BBa_K1690003
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1690003/annotation2464322/range2464322/1

https://synbiohub.org/public/igem/BBa_K1690003/annotation2464322/range2464322/1
type: Range
persistent identity: range2464322
display id: range2464322
version: 1
direction: direction/forward
top level: BBa_K1690003
start: 276
end: 312
orientation: inline

Secretion tag
type: SequenceAnnotation
persistent identity: annotation2464315
display id: annotation2464315
version: 1
top level: BBa_K1690003
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1690003/annotation2464315/range2464315/1

https://synbiohub.org/public/igem/BBa_K1690003/annotation2464315/range2464315/1
type: Range
persistent identity: range2464315
display id: range2464315
version: 1
direction: direction/forward
top level: BBa_K1690003
start: 10
end: 275
orientation: inline

BBa_K1659501
type: ComponentDefinition
persistent identity: BBa K1659501
display id: BBa_K1659501
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pLsr + RBS + sfGFP
creator: Wei Chung Kong
created: 2015-08-27T11:00:00Z
modified: 2015-09-14T09:35:38Z
discontinued: false
dominant: false
group u list: _2077_
m user id: 24524
owner id: 24524
owning group id: 9
status: status/Planning
bookmark: false
mutable description: to be updated
mutable notes: to be updated
mutable provenance: to be updated
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: pLsr (K117002)
sequence annotation: Weak RBS (B0031)
sequence annotation: Superfolder GFP (I746916)
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_K1659501_sequence/1

pLsr (K117002)
type: SequenceAnnotation
persistent identity: annotation2447907
display id: annotation2447907
version: 1
top level: BBa_K1659501
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1659501/annotation2447907/range2447907/1

https://synbiohub.org/public/igem/BBa_K1659501/annotation2447907/range2447907/1
type: Range
persistent identity: range2447907
display id: range2447907
version: 1
direction: direction/forward
top level: BBa_K1659501
start: 1
end: 90
orientation: inline

Weak RBS (B0031)
type: SequenceAnnotation
persistent identity: annotation2447908
display id: annotation2447908
version: 1
top level: BBa_K1659501
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1659501/annotation2447908/range2447908/1

https://synbiohub.org/public/igem/BBa_K1659501/annotation2447908/range2447908/1
type: Range
persistent identity: range2447908
display id: range2447908
version: 1
direction: direction/forward
top level: BBa_K1659501
start: 99
end: 112
orientation: inline

Superfolder GFP (I746916)
type: SequenceAnnotation
persistent identity: annotation2447909
display id: annotation2447909
version: 1
top level: BBa_K1659501
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1659501/annotation2447909/range2447909/1

https://synbiohub.org/public/igem/BBa_K1659501/annotation2447909/range2447909/1
type: Range
persistent identity: range2447909
display id: range2447909
version: 1
direction: direction/forward
top level: BBa_K1659501
start: 119
end: 838
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2447910
display id: annotation2447910
version: 1
top level: BBa_K1659501
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1659501/annotation2447910/range2447910/1

https://synbiohub.org/public/igem/BBa_K1659501/annotation2447910/range2447910/1
type: Range
persistent identity: range2447910
display id: range2447910
version: 1
direction: direction/forward
top level: BBa_K1659501
start: 839
end: 844
orientation: inline

BBa_K1689001
type: ComponentDefinition
persistent identity: BBa K1689001
display id: BBa_K1689001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Coding sequence of STV-N-luc
creator: ZHANG Yihao
created: 2015-09-15T11:00:00Z
modified: 2016-01-19T02:50:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2107_
m user id: 4206
owner id: 26464
owning group id: 9
status: status/Planning
bookmark: false
mutable description: including streptavidin, 2??linker, and firefly luciferase N terminal 416 amino acid domain.
mutable notes: streptavidin and N terminal 416 amino acid domain was linked by 2??linker
mutable provenance: E.coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: streptavidin
sequence annotation: N416 terminal of firefly luciferase
sequence annotation: linker
sequence annotation: start codon
sequence annotation: stop codon
sequence annotation: random spacer
sequence: https://synbiohub.org/public/igem/BBa_K1689001_sequence/1

streptavidin
type: SequenceAnnotation
persistent identity: annotation2469930
display id: annotation2469930
version: 1
top level: BBa_K1689001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1689001/annotation2469930/range2469930/1

https://synbiohub.org/public/igem/BBa_K1689001/annotation2469930/range2469930/1
type: Range
persistent identity: range2469930
display id: range2469930
version: 1
direction: direction/forward
top level: BBa_K1689001
start: 38
end: 391
orientation: inline

N416 terminal of firefly luciferase
type: SequenceAnnotation
persistent identity: annotation2469932
display id: annotation2469932
version: 1
top level: BBa_K1689001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1689001/annotation2469932/range2469932/1

https://synbiohub.org/public/igem/BBa_K1689001/annotation2469932/range2469932/1
type: Range
persistent identity: range2469932
display id: range2469932
version: 1
direction: direction/forward
top level: BBa_K1689001
start: 422
end: 1669
orientation: inline

linker
type: SequenceAnnotation
persistent identity: annotation2469931
display id: annotation2469931
version: 1
top level: BBa_K1689001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1689001/annotation2469931/range2469931/1

https://synbiohub.org/public/igem/BBa_K1689001/annotation2469931/range2469931/1
type: Range
persistent identity: range2469931
display id: range2469931
version: 1
direction: direction/forward
top level: BBa_K1689001
start: 392
end: 421
orientation: inline

start codon
type: SequenceAnnotation
persistent identity: annotation2469928
display id: annotation2469928
version: 1
top level: BBa_K1689001
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1689001/annotation2469928/range2469928/1

https://synbiohub.org/public/igem/BBa_K1689001/annotation2469928/range2469928/1
type: Range
persistent identity: range2469928
display id: range2469928
version: 1
direction: direction/forward
top level: BBa_K1689001
start: 35
end: 37
orientation: inline

stop codon
type: SequenceAnnotation
persistent identity: annotation2469934
display id: annotation2469934
version: 1
top level: BBa_K1689001
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1689001/annotation2469934/range2469934/1

https://synbiohub.org/public/igem/BBa_K1689001/annotation2469934/range2469934/1
type: Range
persistent identity: range2469934
display id: range2469934
version: 1
direction: direction/forward
top level: BBa_K1689001
start: 1670
end: 1672
orientation: inline

random spacer
type: SequenceAnnotation
persistent identity: annotation2477762
display id: annotation2477762
version: 1
top level: BBa_K1689001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1689001/annotation2477762/range2477762/1

https://synbiohub.org/public/igem/BBa_K1689001/annotation2477762/range2477762/1
type: Range
persistent identity: range2477762
display id: range2477762
version: 1
direction: direction/forward
top level: BBa_K1689001
start: 1673
end: 1727
orientation: inline

random spacer
type: SequenceAnnotation
persistent identity: annotation2477761
display id: annotation2477761
version: 1
top level: BBa_K1689001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1689001/annotation2477761/range2477761/1

https://synbiohub.org/public/igem/BBa_K1689001/annotation2477761/range2477761/1
type: Range
persistent identity: range2477761
display id: range2477761
version: 1
direction: direction/forward
top level: BBa_K1689001
start: 1
end: 34
orientation: inline

UNIK Antif
type: ComponentDefinition
persistent identity: BBa K1825000
display id: BBa_K1825000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: UNIK antifreeze protein
creator: Adam Petersen
created: 2015-08-30T11:00:00Z
modified: 2016-01-27T10:30:03Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2251_
m user id: 4206
owner id: 28032
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This is the coding region of a small antifreeze protein. It works by binding to the ice crystals as they form inside the cell, and by doing so prevents the ice crystals from enlarging and penetrating the cell membrane. We wanted to test if there would be a significant difference in the growth rate of moss, Physcomitrella patens, with the antifreeze gene, compared to the one without. This would be done at a low temperature to test the in-vivo function of the antifreeze protein.
mutable notes: We used codon optimization for moss so it would work better in Physcomitrella Patens.
mutable provenance: The part is taken from a spruce budworm, Choristoneura fumiferana, living in areas with permafrost.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: startcodon
sequence: https://synbiohub.org/public/igem/BBa_K1825000_sequence/1

startcodon
type: SequenceAnnotation
persistent identity: annotation2440844
display id: annotation2440844
version: 1
top level: UNIK Antif
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1825000/annotation2440844/range2440844/1

https://synbiohub.org/public/igem/BBa_K1825000/annotation2440844/range2440844/1
type: Range
persistent identity: range2440844
display id: range2440844
version: 1
direction: direction/forward
top level: UNIK Antif
start: 1
end: 3
orientation: inline

BBa_K1799019
type: ComponentDefinition
persistent identity: BBa K1799019
display id: BBa_K1799019
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AHL-induced LacZ Generator
creator: Will Shindel
created: 2015-09-07T11:00:00Z
modified: 2015-09-18T04:12:41Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2224_
m user id: 9151
owner id: 26954
owning group id: 9
status: status/Planning
bookmark: true
mutable description: In the presence of AHL, produces LuxR, which generates a positive feedback loop increasing transcription. LacZ is located downstream of LuxR and is used to measure promoter activity through a β-galactosidase assay.
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K909006
component: BBa_J37019
sequence annotation: BBa_J37019
sequence annotation: BBa_K909006
sequence: https://synbiohub.org/public/igem/BBa_K1799019_sequence/1

BBa_K909006
type: Component
persistent identity: component2447465
display id: component2447465
version: 1
top level: BBa_K1799019
access: public
definition: https://synbiohub.org/public/igem/BBa_K909006/1

BBa_J37019
type: Component
persistent identity: component2447451
display id: component2447451
version: 1
top level: BBa_K1799019
access: public
definition: https://synbiohub.org/public/igem/BBa_J37019/1

BBa_J37019
type: SequenceAnnotation
persistent identity: annotation2447451
display id: annotation2447451
version: 1
top level: BBa_K1799019
location: https://synbiohub.org/public/igem/BBa_K1799019/annotation2447451/range2447451/1
component: BBa_J37019

https://synbiohub.org/public/igem/BBa_K1799019/annotation2447451/range2447451/1
type: Range
persistent identity: range2447451
display id: range2447451
version: 1
direction: direction/reverse
top level: BBa_K1799019
start: 1
end: 862
orientation: inline

BBa_K909006
type: SequenceAnnotation
persistent identity: annotation2447465
display id: annotation2447465
version: 1
top level: BBa_K1799019
location: https://synbiohub.org/public/igem/BBa_K1799019/annotation2447465/range2447465/1
component: BBa_K909006

https://synbiohub.org/public/igem/BBa_K1799019/annotation2447465/range2447465/1
type: Range
persistent identity: range2447465
display id: range2447465
version: 1
direction: direction/reverse
top level: BBa_K1799019
start: 871
end: 4139
orientation: inline

BBa_K1595006
type: ComponentDefinition
persistent identity: BBa K1595006
display id: BBa_K1595006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: EcnB from Escherichia coli
creator: Jonlin Chen
created: 2015-09-04T11:00:00Z
modified: 2015-09-05T09:48:05Z
discontinued: false
dominant: false
group u list: _2012_
m user id: 18839
owner id: 18839
owning group id: 9
status: status/Planning
bookmark: false
mutable description: T7 Promoter + Ribosome binding site + EcnB + 6x Hist tag + terminator. Coding device for anti-microbial peptide derived from Escherichia coli EcnB.
mutable notes: Use in conjunction with strain-expressing T7 polymerase
mutable provenance: Synthesized by Integrated DNA Technologies
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: T7
sequence annotation: RBS
sequence annotation: 6xHist tag
sequence annotation: EcnB
sequence annotation: Terminator
sequence: https://synbiohub.org/public/igem/BBa_K1595006_sequence/1

T7
type: SequenceAnnotation
persistent identity: annotation2444188
display id: annotation2444188
version: 1
top level: BBa_K1595006
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1595006/annotation2444188/range2444188/1

https://synbiohub.org/public/igem/BBa_K1595006/annotation2444188/range2444188/1
type: Range
persistent identity: range2444188
display id: range2444188
version: 1
direction: direction/forward
top level: BBa_K1595006
start: 2
end: 20
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2444187
display id: annotation2444187
version: 1
top level: BBa_K1595006
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1595006/annotation2444187/range2444187/1

https://synbiohub.org/public/igem/BBa_K1595006/annotation2444187/range2444187/1
type: Range
persistent identity: range2444187
display id: range2444187
version: 1
direction: direction/forward
top level: BBa_K1595006
start: 22
end: 80
orientation: inline

6xHist tag
type: SequenceAnnotation
persistent identity: annotation2444185
display id: annotation2444185
version: 1
top level: BBa_K1595006
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1595006/annotation2444185/range2444185/1

https://synbiohub.org/public/igem/BBa_K1595006/annotation2444185/range2444185/1
type: Range
persistent identity: range2444185
display id: range2444185
version: 1
direction: direction/forward
top level: BBa_K1595006
start: 225
end: 242
orientation: inline

EcnB
type: SequenceAnnotation
persistent identity: annotation2444186
display id: annotation2444186
version: 1
top level: BBa_K1595006
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1595006/annotation2444186/range2444186/1

https://synbiohub.org/public/igem/BBa_K1595006/annotation2444186/range2444186/1
type: Range
persistent identity: range2444186
display id: range2444186
version: 1
direction: direction/forward
top level: BBa_K1595006
start: 81
end: 224
orientation: inline

Terminator
type: SequenceAnnotation
persistent identity: annotation2444184
display id: annotation2444184
version: 1
top level: BBa_K1595006
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1595006/annotation2444184/range2444184/1

https://synbiohub.org/public/igem/BBa_K1595006/annotation2444184/range2444184/1
type: Range
persistent identity: range2444184
display id: range2444184
version: 1
direction: direction/forward
top level: BBa_K1595006
start: 246
end: 284
orientation: inline

BBa_K1795010
type: ComponentDefinition
persistent identity: BBa K1795010
display id: BBa_K1795010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23119 gRNA under R0040
creator: Andrew D Halleran, Joseph L Maniaci
created: 2015-09-16T11:00:00Z
modified: 2015-09-17T09:45:40Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2220_
m user id: 27446
owner id: 27446
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This sgRNA sequence was designed to target the Promoter Bba_J23119 with the N20 sequence ttgacagctagctcagtcct . If this gRNA is expressed in a cell with a form of the dCas9 protein, the protein will be targeted to the N20 sequence and repress gene expression. The part is under the control of the promoter Bba_R0040. Promoter Bba_R0040 was chosen because it does not contain a PAM and therefore cannot be inadvertently repressed by dCas9 due to unintentional homology in the N20 sequence. Expression of this part can be repressed if TetR is expressed in the cell without Tetracycline present. If TetR and Tetracycline (or its analog aTc) are both present, the part will be expressed.
mutable notes: We made sure to use a different promoter for this part than the one which the part targets.
mutable provenance: The basic template for the sgRNA sequence was taken from the supplementary information of Qi LS, Larson MH, Gilbert LA, et al. Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression. Cell. 2013;152(5):1173-1183. doi:10.1016/j.cell.2013.02.022.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1795100
component: BBa_K1795110
component: BBa_R0040
component: BBa_B0015
sequence annotation: BBa_K1795110
sequence annotation: BBa_R0040
sequence annotation: BBa_K1795100
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K1795010_sequence/1

BBa_K1795100
type: Component
persistent identity: component2467904
display id: component2467904
version: 1
top level: BBa_K1795010
access: public
definition: https://synbiohub.org/public/igem/BBa_K1795100/1

BBa_K1795110
type: Component
persistent identity: component2467903
display id: component2467903
version: 1
top level: BBa_K1795010
access: public
definition: https://synbiohub.org/public/igem/BBa_K1795110/1

BBa_R0040
type: Component
persistent identity: component2467898
display id: component2467898
version: 1
top level: BBa_K1795010
access: public
definition: p(tetR)

BBa_B0015
type: Component
persistent identity: component2467911
display id: component2467911
version: 1
top level: BBa_K1795010
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_K1795110
type: SequenceAnnotation
persistent identity: annotation2467903
display id: annotation2467903
version: 1
top level: BBa_K1795010
location: https://synbiohub.org/public/igem/BBa_K1795010/annotation2467903/range2467903/1
component: BBa_K1795110

https://synbiohub.org/public/igem/BBa_K1795010/annotation2467903/range2467903/1
type: Range
persistent identity: range2467903
display id: range2467903
version: 1
direction: direction/reverse
top level: BBa_K1795010
start: 55
end: 74
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2467898
display id: annotation2467898
version: 1
top level: BBa_K1795010
location: https://synbiohub.org/public/igem/BBa_K1795010/annotation2467898/range2467898/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K1795010/annotation2467898/range2467898/1
type: Range
persistent identity: range2467898
display id: range2467898
version: 1
direction: direction/forward
top level: BBa_K1795010
start: 1
end: 54
orientation: inline

BBa_K1795100
type: SequenceAnnotation
persistent identity: annotation2467904
display id: annotation2467904
version: 1
top level: BBa_K1795010
location: https://synbiohub.org/public/igem/BBa_K1795010/annotation2467904/range2467904/1
component: BBa_K1795100

https://synbiohub.org/public/igem/BBa_K1795010/annotation2467904/range2467904/1
type: Range
persistent identity: range2467904
display id: range2467904
version: 1
direction: direction/reverse
top level: BBa_K1795010
start: 75
end: 156
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2467911
display id: annotation2467911
version: 1
top level: BBa_K1795010
location: https://synbiohub.org/public/igem/BBa_K1795010/annotation2467911/range2467911/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1795010/annotation2467911/range2467911/1
type: Range
persistent identity: range2467911
display id: range2467911
version: 1
direction: direction/reverse
top level: BBa_K1795010
start: 157
end: 285
orientation: inline

BBa_K1696005
type: ComponentDefinition
persistent identity: BBa K1696005
display id: BBa_K1696005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: P170 Promoter
creator: Yue Liu
created: 2015-09-10T11:00:00Z
modified: 2015-09-11T12:43:19Z
discontinued: false
dominant: false
group u list: _2114_
m user id: 16881
owner id: 16881
owning group id: 9
status: status/Planning
bookmark: false
mutable description: P170 promoter is upregulated at low pH during the transition to stationary phase.P170 was induced between pH 6.5 and pH 6.0.
mutable notes: P170 segment contains a cis-acting sequence involved in the control of promoter regulation.
mutable provenance: L. lactis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: P170 promoter
sequence: https://synbiohub.org/public/igem/BBa_K1696005_sequence/1

P170 promoter
type: SequenceAnnotation
persistent identity: annotation2449400
display id: annotation2449400
version: 1
top level: BBa_K1696005
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1696005/annotation2449400/range2449400/1

https://synbiohub.org/public/igem/BBa_K1696005/annotation2449400/range2449400/1
type: Range
persistent identity: range2449400
display id: range2449400
version: 1
direction: direction/forward
top level: BBa_K1696005
start: 1
end: 418
orientation: inline

BBa_K1763010
type: ComponentDefinition
persistent identity: BBa K1763010
display id: BBa_K1763010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Major Spidroin Protein 1 (MaSp1) with Sticky Ends AB
creator: Vinson Lam
created: 2015-07-26T11:00:00Z
modified: 2015-09-16T10:43:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2185_
m user id: 27136
owner id: 27136
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This part contains the core sequence of MaSp1 that has been assembled with a 5'-AGTT-3' overhang on the 5' end of the sequence and 3'-ACAG-5' overhang on the 3' end of the sequence. This part was designed for use with ICA, along with parts BBa_K1763003 and BBa_K1763004 as an efficient method to assemble multiple MaSp sequences together. This setup is to show that this particular set of sticky ends is suitable for ICA. Before using in ICA, this part should be digested with BsaI, a type IIs restriction enzyme, which cuts DNA outside of its recognition site.
mutable notes: The gene sequence for MaSp2 is particularly GC-rich, which may complicate PCR.
mutable provenance: This part is derived from the MaSp2 gene found in the spider N. Clavipes.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: BsaI recognition site (reverse)
sequence annotation: MaSp1 Core
sequence annotation: BsaI recognition site (forward)
sequence annotation: B sticky end
sequence annotation: A sticky end
sequence: https://synbiohub.org/public/igem/BBa_K1763010_sequence/1

BsaI recognition site (reverse)
type: SequenceAnnotation
persistent identity: annotation2433553
display id: annotation2433553
version: 1
top level: BBa_K1763010
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1763010/annotation2433553/range2433553/1

https://synbiohub.org/public/igem/BBa_K1763010/annotation2433553/range2433553/1
type: Range
persistent identity: range2433553
display id: range2433553
version: 1
direction: direction/forward
top level: BBa_K1763010
start: 114
end: 119
orientation: inline

MaSp1 Core
type: SequenceAnnotation
persistent identity: annotation2473356
display id: annotation2473356
version: 1
top level: BBa_K1763010
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763010/annotation2473356/range2473356/1

https://synbiohub.org/public/igem/BBa_K1763010/annotation2473356/range2473356/1
type: Range
persistent identity: range2473356
display id: range2473356
version: 1
direction: direction/forward
top level: BBa_K1763010
start: 10
end: 108
orientation: inline

BsaI recognition site (forward)
type: SequenceAnnotation
persistent identity: annotation2433552
display id: annotation2433552
version: 1
top level: BBa_K1763010
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1763010/annotation2433552/range2433552/1

https://synbiohub.org/public/igem/BBa_K1763010/annotation2433552/range2433552/1
type: Range
persistent identity: range2433552
display id: range2433552
version: 1
direction: direction/forward
top level: BBa_K1763010
start: 1
end: 5
orientation: inline

B sticky end
type: SequenceAnnotation
persistent identity: annotation2463773
display id: annotation2463773
version: 1
top level: BBa_K1763010
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1763010/annotation2463773/range2463773/1

https://synbiohub.org/public/igem/BBa_K1763010/annotation2463773/range2463773/1
type: Range
persistent identity: range2463773
display id: range2463773
version: 1
direction: direction/forward
top level: BBa_K1763010
start: 109
end: 112
orientation: inline

A sticky end
type: SequenceAnnotation
persistent identity: annotation2463772
display id: annotation2463772
version: 1
top level: BBa_K1763010
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1763010/annotation2463772/range2463772/1

https://synbiohub.org/public/igem/BBa_K1763010/annotation2463772/range2463772/1
type: Range
persistent identity: range2463772
display id: range2463772
version: 1
direction: direction/forward
top level: BBa_K1763010
start: 7
end: 10
orientation: inline

BBa_K1602046
type: ComponentDefinition
persistent identity: BBa K1602046
display id: BBa_K1602046
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RRK3
creator: Christian Sator,Stefan Zens, Max Zander, Benedikt Spannenkrebs, Sebastian Jaeger
created: 2015-09-15T11:00:00Z
modified: 2015-09-25T05:16:35Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _2019_
m user id: 0
m user id: 23028
owner id: 26220
owning group id: 9
sample status: In stock
status: status/Available
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K808000
component: BBa_B0015
component: BBa_J01086
sequence annotation: BBa_J01086
sequence annotation: BBa_B0015
sequence annotation: BBa_K808000
sequence: https://synbiohub.org/public/igem/BBa_K1602046_sequence/1

BBa_K808000
type: Component
persistent identity: component2462830
display id: component2462830
version: 1
top level: BBa_K1602046
access: public
definition: https://synbiohub.org/public/igem/BBa_K808000/1

BBa_B0015
type: Component
persistent identity: component2462838
display id: component2462838
version: 1
top level: BBa_K1602046
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_J01086
type: Component
persistent identity: component2462831
display id: component2462831
version: 1
top level: BBa_K1602046
access: public
definition: https://synbiohub.org/public/igem/BBa_J01086/1

BBa_J01086
type: SequenceAnnotation
persistent identity: annotation2462831
display id: annotation2462831
version: 1
top level: BBa_K1602046
location: https://synbiohub.org/public/igem/BBa_K1602046/annotation2462831/range2462831/1
component: BBa_J01086

https://synbiohub.org/public/igem/BBa_K1602046/annotation2462831/range2462831/1
type: Range
persistent identity: range2462831
display id: range2462831
version: 1
direction: direction/reverse
top level: BBa_K1602046
start: 1218
end: 1311
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2462838
display id: annotation2462838
version: 1
top level: BBa_K1602046
location: https://synbiohub.org/public/igem/BBa_K1602046/annotation2462838/range2462838/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1602046/annotation2462838/range2462838/1
type: Range
persistent identity: range2462838
display id: range2462838
version: 1
direction: direction/reverse
top level: BBa_K1602046
start: 1320
end: 1448
orientation: inline

BBa_K808000
type: SequenceAnnotation
persistent identity: annotation2462830
display id: annotation2462830
version: 1
top level: BBa_K1602046
location: https://synbiohub.org/public/igem/BBa_K1602046/annotation2462830/range2462830/1
component: BBa_K808000

https://synbiohub.org/public/igem/BBa_K1602046/annotation2462830/range2462830/1
type: Range
persistent identity: range2462830
display id: range2462830
version: 1
direction: direction/reverse
top level: BBa_K1602046
start: 1
end: 1209
orientation: inline

BBa_K1763009
type: ComponentDefinition
persistent identity: BBa K1763009
display id: BBa_K1763009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Major Spidroin Protein 2 (MaSp2) Sequencing Core with AB Sticky Ends
creator: Vinson Lam
created: 2015-07-26T11:00:00Z
modified: 2015-09-16T10:43:11Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2185_
m user id: 27136
owner id: 27136
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This part contains the core sequence of MaSp2 that has been assembled with a 5'-AGTT-3' overhang on the 5' end of the sequence and 3'-ACAG-5' overhang on the 3' end of the sequence. This part was designed for use with ICA, along with parts BBa_K1763003 and BBa_K1763004 as an efficient method to assemble multiple MaSp2 sequences together. This setup is to show that this particular set of sticky ends is suitable for ICA. Before using in ICA, this part should be digested with BsaI, a type IIs restriction enzyme, which cuts DNA outside of its recognition site. This part codes for identical protein primary sequence as BBa_K17630002, but has a different DNA sequence. This part is intended to anneal to a sequencing primer that will allow longer composite MaSp genes to be read.
mutable notes: The gene sequence for MaSp2 is particularly GC-rich, which may complicate PCR.
mutable provenance: This part is derived from the MaSp2 gene found in the spider N. Clavipes.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: BsaI recognition site(reverse)
sequence annotation: SeqF Primer Binding Site
sequence annotation: A sticky end
sequence annotation: BsaI recognition site (forward)
sequence annotation: MaSp2 Core Sequence
sequence annotation: SeqR Primer Binding Site
sequence annotation: B sticky end
sequence: https://synbiohub.org/public/igem/BBa_K1763009_sequence/1

BsaI recognition site(reverse)
type: SequenceAnnotation
persistent identity: annotation2454683
display id: annotation2454683
version: 1
top level: BBa_K1763009
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1763009/annotation2454683/range2454683/1

https://synbiohub.org/public/igem/BBa_K1763009/annotation2454683/range2454683/1
type: Range
persistent identity: range2454683
display id: range2454683
version: 1
direction: direction/reverse
top level: BBa_K1763009
start: 114
end: 119
orientation: inline

SeqF Primer Binding Site
type: SequenceAnnotation
persistent identity: annotation2473347
display id: annotation2473347
version: 1
top level: BBa_K1763009
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K1763009/annotation2473347/range2473347/1

https://synbiohub.org/public/igem/BBa_K1763009/annotation2473347/range2473347/1
type: Range
persistent identity: range2473347
display id: range2473347
version: 1
direction: direction/forward
top level: BBa_K1763009
start: 47
end: 64
orientation: inline

A sticky end
type: SequenceAnnotation
persistent identity: annotation2463770
display id: annotation2463770
version: 1
top level: BBa_K1763009
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1763009/annotation2463770/range2463770/1

https://synbiohub.org/public/igem/BBa_K1763009/annotation2463770/range2463770/1
type: Range
persistent identity: range2463770
display id: range2463770
version: 1
direction: direction/forward
top level: BBa_K1763009
start: 7
end: 10
orientation: inline

BsaI recognition site (forward)
type: SequenceAnnotation
persistent identity: annotation2433551
display id: annotation2433551
version: 1
top level: BBa_K1763009
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1763009/annotation2433551/range2433551/1

https://synbiohub.org/public/igem/BBa_K1763009/annotation2433551/range2433551/1
type: Range
persistent identity: range2433551
display id: range2433551
version: 1
direction: direction/forward
top level: BBa_K1763009
start: 1
end: 5
orientation: inline

MaSp2 Core Sequence
type: SequenceAnnotation
persistent identity: annotation2473130
display id: annotation2473130
version: 1
top level: BBa_K1763009
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763009/annotation2473130/range2473130/1

https://synbiohub.org/public/igem/BBa_K1763009/annotation2473130/range2473130/1
type: Range
persistent identity: range2473130
display id: range2473130
version: 1
direction: direction/forward
top level: BBa_K1763009
start: 10
end: 108
orientation: inline

SeqR Primer Binding Site
type: SequenceAnnotation
persistent identity: annotation2473348
display id: annotation2473348
version: 1
top level: BBa_K1763009
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K1763009/annotation2473348/range2473348/1

https://synbiohub.org/public/igem/BBa_K1763009/annotation2473348/range2473348/1
type: Range
persistent identity: range2473348
display id: range2473348
version: 1
direction: direction/reverse
top level: BBa_K1763009
start: 47
end: 64
orientation: inline

B sticky end
type: SequenceAnnotation
persistent identity: annotation2463771
display id: annotation2463771
version: 1
top level: BBa_K1763009
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1763009/annotation2463771/range2463771/1

https://synbiohub.org/public/igem/BBa_K1763009/annotation2463771/range2463771/1
type: Range
persistent identity: range2463771
display id: range2463771
version: 1
direction: direction/forward
top level: BBa_K1763009
start: 109
end: 112
orientation: inline

BBa_K1723005
type: ComponentDefinition
persistent identity: BBa K1723005
display id: BBa_K1723005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PAM rich URS J23117Alt promoter
creator: Emilie Cuillery
created: 2015-09-14T11:00:00Z
modified: 2015-09-15T10:48:08Z
discontinued: false
dominant: false
group u list: _2143_
m user id: 26018
owner id: 26018
owning group id: 9
status: status/Planning
bookmark: false
mutable description: PAM rich URS J23117 is a promoter made from the existing PAM rich URS J23117 promoter (BBa_K1723001) and mutated. This promoter has PAM (PAM = NGG sequence) rich Upstream Regulatory Sequence to enable the use of protein dCas9-ω (BBa_K1723000) as a gene transcription regulator when in complex with one sgRNA targeting the promoter such as: X0 (BBa_K1723006), X4 (BBa_K1723007), or X35 (BBa_K1723008). Discover all the parts that can work with this one: http://2015.igem.org/Team:EPF_Lausanne/Part_Collection
mutable notes: This part was synthesized on the model of the PAM rich URS J23117 promoter. It was obtained by mutating bases on the promoter to obtain different target sites for sgRNAs for promoter regulation using dCas9-ω system.
mutable provenance: this part was fully synthesized.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: PAM
sequence annotation: J23117Alt
sequence annotation: sgRNA X4 recognition site
sequence annotation: sgRNA X35 recognition site
sequence annotation: PAM rich URS
sequence annotation: sgRNA X0 recognition site
sequence annotation: -10 sequence
sequence annotation: -35 sequence
sequence: https://synbiohub.org/public/igem/BBa_K1723005_sequence/1

PAM
type: SequenceAnnotation
persistent identity: annotation2457072
display id: annotation2457072
version: 1
top level: BBa_K1723005
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1723005/annotation2457072/range2457072/1

https://synbiohub.org/public/igem/BBa_K1723005/annotation2457072/range2457072/1
type: Range
persistent identity: range2457072
display id: range2457072
version: 1
direction: direction/forward
top level: BBa_K1723005
start: 269
end: 271
orientation: inline

J23117Alt
type: SequenceAnnotation
persistent identity: annotation2457067
display id: annotation2457067
version: 1
top level: BBa_K1723005
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1723005/annotation2457067/range2457067/1

https://synbiohub.org/public/igem/BBa_K1723005/annotation2457067/range2457067/1
type: Range
persistent identity: range2457067
display id: range2457067
version: 1
direction: direction/forward
top level: BBa_K1723005
start: 248
end: 282
orientation: inline

PAM
type: SequenceAnnotation
persistent identity: annotation2457068
display id: annotation2457068
version: 1
top level: BBa_K1723005
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1723005/annotation2457068/range2457068/1

https://synbiohub.org/public/igem/BBa_K1723005/annotation2457068/range2457068/1
type: Range
persistent identity: range2457068
display id: range2457068
version: 1
direction: direction/reverse
top level: BBa_K1723005
start: 189
end: 191
orientation: inline

sgRNA X4 recognition site
type: SequenceAnnotation
persistent identity: annotation2457069
display id: annotation2457069
version: 1
top level: BBa_K1723005
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1723005/annotation2457069/range2457069/1

https://synbiohub.org/public/igem/BBa_K1723005/annotation2457069/range2457069/1
type: Range
persistent identity: range2457069
display id: range2457069
version: 1
direction: direction/reverse
top level: BBa_K1723005
start: 192
end: 211
orientation: inline

sgRNA X35 recognition site
type: SequenceAnnotation
persistent identity: annotation2457071
display id: annotation2457071
version: 1
top level: BBa_K1723005
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1723005/annotation2457071/range2457071/1

https://synbiohub.org/public/igem/BBa_K1723005/annotation2457071/range2457071/1
type: Range
persistent identity: range2457071
display id: range2457071
version: 1
direction: direction/reverse
top level: BBa_K1723005
start: 245
end: 264
orientation: inline

PAM rich URS
type: SequenceAnnotation
persistent identity: annotation2457066
display id: annotation2457066
version: 1
top level: BBa_K1723005
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1723005/annotation2457066/range2457066/1

https://synbiohub.org/public/igem/BBa_K1723005/annotation2457066/range2457066/1
type: Range
persistent identity: range2457066
display id: range2457066
version: 1
direction: direction/forward
top level: BBa_K1723005
start: 1
end: 247
orientation: inline

sgRNA X0 recognition site
type: SequenceAnnotation
persistent identity: annotation2457074
display id: annotation2457074
version: 1
top level: BBa_K1723005
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1723005/annotation2457074/range2457074/1

https://synbiohub.org/public/igem/BBa_K1723005/annotation2457074/range2457074/1
type: Range
persistent identity: range2457074
display id: range2457074
version: 1
direction: direction/forward
top level: BBa_K1723005
start: 249
end: 268
orientation: inline

PAM
type: SequenceAnnotation
persistent identity: annotation2457070
display id: annotation2457070
version: 1
top level: BBa_K1723005
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1723005/annotation2457070/range2457070/1

https://synbiohub.org/public/igem/BBa_K1723005/annotation2457070/range2457070/1
type: Range
persistent identity: range2457070
display id: range2457070
version: 1
direction: direction/reverse
top level: BBa_K1723005
start: 242
end: 244
orientation: inline

-10 sequence
type: SequenceAnnotation
persistent identity: annotation2462284
display id: annotation2462284
version: 1
top level: BBa_K1723005
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1723005/annotation2462284/range2462284/1

https://synbiohub.org/public/igem/BBa_K1723005/annotation2462284/range2462284/1
type: Range
persistent identity: range2462284
display id: range2462284
version: 1
direction: direction/forward
top level: BBa_K1723005
start: 271
end: 276
orientation: inline

-35 sequence
type: SequenceAnnotation
persistent identity: annotation2462285
display id: annotation2462285
version: 1
top level: BBa_K1723005
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1723005/annotation2462285/range2462285/1

https://synbiohub.org/public/igem/BBa_K1723005/annotation2462285/range2462285/1
type: Range
persistent identity: range2462285
display id: range2462285
version: 1
direction: direction/forward
top level: BBa_K1723005
start: 248
end: 253
orientation: inline

BBa_K1806003
type: ComponentDefinition
persistent identity: BBa K1806003
display id: BBa_K1806003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7+ ibPB RNA Thermometer + lacI
creator: İBRAHİM YASİR ORHAN
created: 2015-09-16T11:00:00Z
modified: 2015-09-17T07:15:35Z
discontinued: false
dominant: false
group u list: _973_2231_
m user id: 11153
owner id: 11153
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Designed to close lacO present systems in higher temperatures by producing lacI.
mutable notes: none
mutable provenance: The part is a composite part formed up of several distinct parts.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence: https://synbiohub.org/public/igem/BBa_K1806003_sequence/1

BBa_K1799009
type: ComponentDefinition
persistent identity: BBa K1799009
display id: BBa_K1799009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LsrD (modularization optimized)
creator: Michael Flanagan
created: 2015-08-06T11:00:00Z
modified: 2015-09-18T05:16:59Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2224_
m user id: 26720
owner id: 19613
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Gene for the D subunit of the LsrACDB transporter, present in E. coli on the LsrACDBFG operon. The D subunit is a permease protein in the transporter, which actively brings autoinducer 2 (AI-2) into the cell. The portion of the LsrACDBFG operon that codes for the AI-2 transporter consists of four open reading frames, which code for A, C, D, and B, respectively. Three of these ORFs overlap with one another: C overlaps with A, and its RBS is contained within the A subunit ORF, and D overlaps with C (although to a lesser extent), and its RBS is contained within the C subunit ORF. From a modularization standpoint, this creates an inefficiency because the wild type ORFs of the A and C subunits both contain functional RBSs, and in the case of the A subunit ORF, an ATG 4bp downstream. To reduce this inefficiency, we have created versions of each subunit ORF that are optimized for modularization.
mutable notes: This version of the open reading frame has been optimized for modularization via silent mutations in segments of code sharing more than 83% homology with the Shine-Dalgarno sequence. It is otherwise identical to the wild type LsrD gene (http://parts.igem.org/Part:BBa_K1799005).
mutable provenance: This part was synthesized, and is based on E. coli genomic data retrieved via EcoGene: http://www.ecogene.org/gene/EG13808
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1799009_sequence/1

BBa_K1799011
type: ComponentDefinition
persistent identity: BBa K1799011
display id: BBa_K1799011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LsrA RBS
creator: Will Shindel
created: 2015-08-26T11:00:00Z
modified: 2015-08-27T03:05:53Z
discontinued: false
dominant: false
group u list: _2224_
m user id: 26954
owner id: 26954
owning group id: 9
status: status/Planning
bookmark: false
mutable description: RBS found upstream of the LsrA open reading frame in E. coli.
mutable notes: None.
mutable provenance: This part was synthesized, and is based on E. coli genomic data retrieved via EcoGene: http://www.ecogene.org/gene/EG13809
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_K1799011_sequence/1

tEno2
type: ComponentDefinition
persistent identity: BBa K1829003
display id: BBa_K1829003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: tEno2: Yeast terminator
creator: Nicholas Suen
created: 2015-09-09T11:00:00Z
modified: 2015-09-17T02:28:03Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2255_
m user id: 27842
owner id: 27842
owning group id: 9
status: status/Planning
bookmark: false
mutable description: tENO2 is a terminator
mutable notes: cats
mutable provenance: cats
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence: https://synbiohub.org/public/igem/BBa_K1829003_sequence/1

BBa_K1799010
type: ComponentDefinition
persistent identity: BBa K1799010
display id: BBa_K1799010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LsrB (modularization optimized)
creator: Michael Flanagan
created: 2015-08-06T11:00:00Z
modified: 2015-09-18T05:17:34Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2224_
m user id: 26720
owner id: 19613
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Gene for the B subunit of the LsrACDB transporter, present in E. coli on the LsrACDBFG operon. The B subunit is the autoinducer 2 (AI-2) binding protein in the transporter, which actively brings AI-2 into the cell. The portion of the LsrACDBFG operon that codes for the AI-2 transporter consists of four open reading frames, which code for A, C, D, and B, respectively. Three of these ORFs overlap with one another: C overlaps with A, and its RBS is contained within the A subunit ORF, and D overlaps with C (although to a lesser extent), and its RBS is contained within the C subunit ORF. From a modularization standpoint, this creates an inefficiency because the wild type ORFs of the A and C subunits both contain functional RBSs, and in the case of the A subunit ORF, an ATG 4bp downstream. To reduce this inefficiency, we have created versions of each subunit ORF that are optimized for modularization.
mutable notes: This version of the open reading frame has been optimized for modularization via silent mutations in segments of code sharing more than 83% homology with the Shine-Dalgarno sequence. It is otherwise identical to the wild type LsrB gene (http://parts.igem.org/Part:BBa_K1799006).
mutable provenance: This part was synthesized, and is based on E. coli genomic data retrieved via EcoGene: http://www.ecogene.org/gene/EG13809
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1799010_sequence/1

BBa_K1653006
type: ComponentDefinition
persistent identity: BBa K1653006
display id: BBa_K1653006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: MarA
creator: Yoshiharu Otaki, Daiki Haraguchi
created: 2015-05-30T11:00:00Z
modified: 2015-09-18T04:00:50Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2071_
m user id: 22434
owner id: 22434
owning group id: 9
status: status/Planning
bookmark: false
mutable description: next time
mutable notes: next time
mutable provenance: next time
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: MarA
sequence: https://synbiohub.org/public/igem/BBa_K1653006_sequence/1

MarA
type: SequenceAnnotation
persistent identity: annotation2432405
display id: annotation2432405
version: 1
top level: BBa_K1653006
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1653006/annotation2432405/range2432405/1

https://synbiohub.org/public/igem/BBa_K1653006/annotation2432405/range2432405/1
type: Range
persistent identity: range2432405
display id: range2432405
version: 1
direction: direction/forward
top level: BBa_K1653006
start: 1
end: 384
orientation: inline

BBa_K1640008
type: ComponentDefinition
persistent identity: BBa K1640008
display id: BBa_K1640008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: psbMZH
creator: Meghan Cook
created: 2015-09-09T11:00:00Z
modified: 2015-09-18T08:53:22Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2057_
m user id: 28565
owner id: 28565
owning group id: 9
status: status/Planning
bookmark: true
mutable description: N/a
mutable notes: N/a
mutable provenance: N/a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: psbH
sequence annotation: rbs
sequence annotation: psbZ
sequence annotation: Plac
sequence annotation: psbM
sequence: https://synbiohub.org/public/igem/BBa_K1640008_sequence/1

psbH
type: SequenceAnnotation
persistent identity: annotation2448250
display id: annotation2448250
version: 1
top level: BBa_K1640008
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1640008/annotation2448250/range2448250/1

https://synbiohub.org/public/igem/BBa_K1640008/annotation2448250/range2448250/1
type: Range
persistent identity: range2448250
display id: range2448250
version: 1
direction: direction/forward
top level: BBa_K1640008
start: 321
end: 488
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2448245
display id: annotation2448245
version: 1
top level: BBa_K1640008
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1640008/annotation2448245/range2448245/1

https://synbiohub.org/public/igem/BBa_K1640008/annotation2448245/range2448245/1
type: Range
persistent identity: range2448245
display id: range2448245
version: 1
direction: direction/forward
top level: BBa_K1640008
start: 41
end: 57
orientation: inline

psbZ
type: SequenceAnnotation
persistent identity: annotation2448248
display id: annotation2448248
version: 1
top level: BBa_K1640008
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1640008/annotation2448248/range2448248/1

https://synbiohub.org/public/igem/BBa_K1640008/annotation2448248/range2448248/1
type: Range
persistent identity: range2448248
display id: range2448248
version: 1
direction: direction/forward
top level: BBa_K1640008
start: 176
end: 307
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2448249
display id: annotation2448249
version: 1
top level: BBa_K1640008
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1640008/annotation2448249/range2448249/1

https://synbiohub.org/public/igem/BBa_K1640008/annotation2448249/range2448249/1
type: Range
persistent identity: range2448249
display id: range2448249
version: 1
direction: direction/forward
top level: BBa_K1640008
start: 308
end: 320
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2448247
display id: annotation2448247
version: 1
top level: BBa_K1640008
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1640008/annotation2448247/range2448247/1

https://synbiohub.org/public/igem/BBa_K1640008/annotation2448247/range2448247/1
type: Range
persistent identity: range2448247
display id: range2448247
version: 1
direction: direction/forward
top level: BBa_K1640008
start: 163
end: 175
orientation: inline

Plac
type: SequenceAnnotation
persistent identity: annotation2448244
display id: annotation2448244
version: 1
top level: BBa_K1640008
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1640008/annotation2448244/range2448244/1

https://synbiohub.org/public/igem/BBa_K1640008/annotation2448244/range2448244/1
type: Range
persistent identity: range2448244
display id: range2448244
version: 1
direction: direction/forward
top level: BBa_K1640008
start: 1
end: 40
orientation: inline

psbM
type: SequenceAnnotation
persistent identity: annotation2448246
display id: annotation2448246
version: 1
top level: BBa_K1640008
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1640008/annotation2448246/range2448246/1

https://synbiohub.org/public/igem/BBa_K1640008/annotation2448246/range2448246/1
type: Range
persistent identity: range2448246
display id: range2448246
version: 1
direction: direction/forward
top level: BBa_K1640008
start: 58
end: 162
orientation: inline

BBa_K1723000
type: ComponentDefinition
persistent identity: BBa K1723000
display id: BBa_K1723000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: dCas9-ω
creator: Gregoire Thouvenin
created: 2015-08-09T11:00:00Z
modified: 2015-08-10T10:13:34Z
discontinued: false
dominant: false
group u list: _2143_
m user id: 26022
owner id: 26022
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This part codes for a enzymatically inactive Cas9 protein (dCas9) that is fused to rpoZ, the omega subunit of E.Coli RNA Polymerase (RNAP). Cas9 (CRISPR associated protein 9) is an RNA-guided DNA endonuclease that targets and cleaves any DNA sequence complementary to its guide RNA (gRNA). Catalytically ???dead??? Cas9 (dCas9) lack the ability to cleave DNA. When fused to a RNAP recruiting element (in this case the omega subunit of RNAP), chimeric dCas9 can act as a programmable transcription activator. dCas9 may also act as a DNA transcription inhibitor: depending on its gRNA-determined binding site, it has been shown in yeasts to sterically hinder RNAP recruitment to promoter sequences.
mutable notes: The original dCas9-ω given to us by the Bikard lab contains an EcoRI restriction site. We performed a site-directed mutagenesis to remove it.
mutable provenance: A plasmid containing dCas9_ω (pdCas9) was given to our iGEM team by David Bikard.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: linker
sequence annotation: rpoZ (omega subunit)
sequence annotation: dCas9
sequence: https://synbiohub.org/public/igem/BBa_K1723000_sequence/1

linker
type: SequenceAnnotation
persistent identity: annotation2434967
display id: annotation2434967
version: 1
top level: BBa_K1723000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1723000/annotation2434967/range2434967/1

https://synbiohub.org/public/igem/BBa_K1723000/annotation2434967/range2434967/1
type: Range
persistent identity: range2434967
display id: range2434967
version: 1
direction: direction/forward
top level: BBa_K1723000
start: 4105
end: 4113
orientation: inline

rpoZ (omega subunit)
type: SequenceAnnotation
persistent identity: annotation2434968
display id: annotation2434968
version: 1
top level: BBa_K1723000
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1723000/annotation2434968/range2434968/1

https://synbiohub.org/public/igem/BBa_K1723000/annotation2434968/range2434968/1
type: Range
persistent identity: range2434968
display id: range2434968
version: 1
direction: direction/forward
top level: BBa_K1723000
start: 4114
end: 4383
orientation: inline

dCas9
type: SequenceAnnotation
persistent identity: annotation2434969
display id: annotation2434969
version: 1
top level: BBa_K1723000
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1723000/annotation2434969/range2434969/1

https://synbiohub.org/public/igem/BBa_K1723000/annotation2434969/range2434969/1
type: Range
persistent identity: range2434969
display id: range2434969
version: 1
direction: direction/forward
top level: BBa_K1723000
start: 1
end: 4104
orientation: inline

BBa_K1763425
type: ComponentDefinition
persistent identity: BBa K1763425
display id: BBa_K1763425
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: MaSp2-3(T7)
creator: Vinson Lam
created: 2015-09-14T11:00:00Z
modified: 2015-09-15T12:19:04Z
discontinued: false
dominant: false
group u list: _2185_
m user id: 27136
owner id: 27136
owning group id: 9
status: status/Planning
bookmark: false
mutable description: MaSp2 3-mer in a T7-RBS expression vector (BBa_K552998)
mutable notes: no.
mutable provenance: Nephila clavipes
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1763424
component: BBa_K525998
sequence annotation: BBa_K525998
sequence annotation: BBa_K1763424
sequence: https://synbiohub.org/public/igem/BBa_K1763425_sequence/1

BBa_K1763424
type: Component
persistent identity: component2456225
display id: component2456225
version: 1
top level: BBa_K1763425
access: public
definition: BBa_K1763424

BBa_K525998
type: Component
persistent identity: component2456224
display id: component2456224
version: 1
top level: BBa_K1763425
access: public
definition: https://synbiohub.org/public/igem/BBa_K525998/1

BBa_K525998
type: SequenceAnnotation
persistent identity: annotation2456224
display id: annotation2456224
version: 1
top level: BBa_K1763425
location: https://synbiohub.org/public/igem/BBa_K1763425/annotation2456224/range2456224/1
component: BBa_K525998

https://synbiohub.org/public/igem/BBa_K1763425/annotation2456224/range2456224/1
type: Range
persistent identity: range2456224
display id: range2456224
version: 1
direction: direction/reverse
top level: BBa_K1763425
start: 1
end: 32
orientation: inline

BBa_K1763424
type: SequenceAnnotation
persistent identity: annotation2456225
display id: annotation2456225
version: 1
top level: BBa_K1763425
location: https://synbiohub.org/public/igem/BBa_K1763425/annotation2456225/range2456225/1
component: BBa_K1763424

https://synbiohub.org/public/igem/BBa_K1763425/annotation2456225/range2456225/1
type: Range
persistent identity: range2456225
display id: range2456225
version: 1
direction: direction/reverse
top level: BBa_K1763425
start: 39
end: 395
orientation: inline

pBar1
type: ComponentDefinition
persistent identity: BBa K1829001
display id: BBa_K1829001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pBar1: Mating factor alpha responsive promoter
creator: Nicholas Suen
created: 2015-09-07T11:00:00Z
modified: 2015-09-14T11:39:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2255_
m user id: 18557
owner id: 27842
owning group id: 9
status: status/Planning
bookmark: false
mutable description: The pBar1 promoter is a mating factor alpha responsive promoter found in MATa S. Cerevisiae. This promoter drives expression of Bar1, a protease that cleaves mating factor alpha.
mutable notes: We changed nucleotide 210 from thymine to cytosine. This does not change the amino acid Asparagine and is therefore a silent mutation. We also changed nucleotide 285 from cytosine to adenine. This does not change the amino acid valine and is therefore a silent mutation.
mutable provenance: pBar1 is found in the genomic sequence of MATa S. cerevisiae.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K1829001_sequence/1

BBa_K1666001
type: ComponentDefinition
persistent identity: BBa K1666001
display id: BBa_K1666001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LsrB of LuxS/AI-2 signaling pathway in Salmonalla
creator: Heming Wang
created: 2015-09-05T11:00:00Z
modified: 2015-09-06T08:09:30Z
discontinued: false
dominant: false
group u list: _2084_
m user id: 23948
owner id: 23948
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Quorum sensing is a process of bacterial cell-to-cell communication involving the production and detection of extracellular signaling molecules called autoinducers. And autoinducer-2 (AI-2) has been proposed to serve as a 'universal signal' for interspecies communication. In the LuxS/AI-2 signaling system of Salmonella Typhimurium, AI-2 response involves ATP binding cassette transporter encoded by genes named Lsr (LuxS regulated). And LsrB a solute-binding protein which could transport the AI-2 into the cytoplasm. In our project, we set this protein-coding part under a nisA promoter and try to integrate them in the genome of Lactobacillus or Lactococcus for the final purpose of constructing an integrated AI-2 response pathway of Salmonella in the engineered bacteria.
mutable notes: The lsrB gene was isolated from the genome of Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 using PCR. We added restriction enzyme sites when designing primers, and the assembly is done through the use of restriction sites (cutting and ligating).
mutable provenance: We extracted the genomic DNA of Salmonella enterica subsp. enterica serovar Typhimurium str. LT2, and used PCR to isolate the gene LsrA(Gene ID: 1255604).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: lsrB
sequence: https://synbiohub.org/public/igem/BBa_K1666001_sequence/1

lsrB
type: SequenceAnnotation
persistent identity: annotation2445157
display id: annotation2445157
version: 1
top level: BBa_K1666001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1666001/annotation2445157/range2445157/1

https://synbiohub.org/public/igem/BBa_K1666001/annotation2445157/range2445157/1
type: Range
persistent identity: range2445157
display id: range2445157
version: 1
direction: direction/forward
top level: BBa_K1666001
start: 1
end: 1023
orientation: inline

Mcfp3
type: ComponentDefinition
persistent identity: BBa K1592001
display id: BBa_K1592001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Mytilus californianus foot protein 3(Mcfp3) variant 3
creator: Shuyan Tang
created: 2015-08-29T11:00:00Z
modified: 2015-09-18T09:23:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2009_
m user id: 20267
owner id: 20267
owning group id: 9
status: status/Planning
bookmark: true
mutable description: This is the cell display system of Yarrowia lipolytica, composed of LIP prepro, interest protein, and YLcwp3. LIP prepro is signal peptide used to secrete the interest protein out of the cell, and the YLcwp3 is the anchor domain binding the interest protein to the cell wall of yeast. We use this system to display silica-tag and test its binding characteristics. E.coli ribosomal protein L2 was found to bind tightly to silicon particles, which have surfaces that are oxidized to silica. This L2 silica-binding tag, called the ??????Si-tag,?????? can be used for one-step targeting of functional proteins on silica surfaces. The silica-binding domains of E. coli L2 was mapped to amino acids 1???60, 61-202 and 203???273. We respectively test the silica-binding characteristics of this three regions and their combinations. E. coli ribosomal protein L2 (1-60) is the amino acids 1???60 of E. coli ribosomal protein L2, we call it si-tag1.
mutable notes: No.
mutable provenance: We got the LIP prepro and YLcwp3 sequence from our lab, and synthetize the E. coli ribosomal protein L2 (1-60) by IDT.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Start codon
sequence: https://synbiohub.org/public/igem/BBa_K1592001_sequence/1

Start codon
type: SequenceAnnotation
persistent identity: annotation2455905
display id: annotation2455905
version: 1
top level: Mcfp3
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1592001/annotation2455905/range2455905/1

https://synbiohub.org/public/igem/BBa_K1592001/annotation2455905/range2455905/1
type: Range
persistent identity: range2455905
display id: range2455905
version: 1
direction: direction/forward
top level: Mcfp3
start: 1
end: 3
orientation: inline

MccS
type: ComponentDefinition
persistent identity: BBa K1659100
display id: BBa_K1659100
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Microcin S, a Class II microcin produced by probiotic E. coli G3/10
creator: Raphaella Hull
created: 2015-08-09T11:00:00Z
modified: 2016-02-03T10:51:08Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2077_
m user id: 4206
owner id: 24525
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Microcins are antibacterial peptides. Microcin S was isolated from Escherichia coli G3/10, a component of the probiotic drug Symbioflor 2. Here it was shown to be responsible for E. coli G3/10's ability to suppress adherence of enteropathogenic E. coli E2348/69. [1] The Microcin S operon is about 4.7 kb in size and is comprised of four genes. This part is the sequencing encoding the microcin itself (mcsS). [1] Zsch??ttig, A., Zimmermann, K., Blom, J., Goesmann, A., P??hlmann, C., & Gunzer, F. (2012). Identification and characterization of microcin S, a new antibacterial peptide produced by probiotic Escherichia coli G3/10. PLoS ONE, 7(3), 1???9. http://doi.org/10.1371/journal.pone.0033351
mutable notes: No specific design considerations
mutable provenance: UniProtKB - H9ZMG7 (H9ZMG7_ECOLX)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Microcin S
sequence annotation: stop
sequence annotation: Hisx6
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_K1659100_sequence/1

Microcin S
type: SequenceAnnotation
persistent identity: annotation2449244
display id: annotation2449244
version: 1
top level: MccS
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1659100/annotation2449244/range2449244/1

https://synbiohub.org/public/igem/BBa_K1659100/annotation2449244/range2449244/1
type: Range
persistent identity: range2449244
display id: range2449244
version: 1
direction: direction/forward
top level: MccS
start: 4
end: 360
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2449246
display id: annotation2449246
version: 1
top level: MccS
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1659100/annotation2449246/range2449246/1

https://synbiohub.org/public/igem/BBa_K1659100/annotation2449246/range2449246/1
type: Range
persistent identity: range2449246
display id: range2449246
version: 1
direction: direction/forward
top level: MccS
start: 379
end: 384
orientation: inline

Hisx6
type: SequenceAnnotation
persistent identity: annotation2449245
display id: annotation2449245
version: 1
top level: MccS
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1659100/annotation2449245/range2449245/1

https://synbiohub.org/public/igem/BBa_K1659100/annotation2449245/range2449245/1
type: Range
persistent identity: range2449245
display id: range2449245
version: 1
direction: direction/forward
top level: MccS
start: 361
end: 378
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2449243
display id: annotation2449243
version: 1
top level: MccS
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1659100/annotation2449243/range2449243/1

https://synbiohub.org/public/igem/BBa_K1659100/annotation2449243/range2449243/1
type: Range
persistent identity: range2449243
display id: range2449243
version: 1
direction: direction/forward
top level: MccS
start: 1
end: 3
orientation: inline

BBa_K1847011
type: ComponentDefinition
persistent identity: BBa K1847011
display id: BBa_K1847011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: lldRO1-placUV5-lacO-lldRO2
creator: Anna Fomitcheva, Lisa Baumgartner, Charlotte Ramon
created: 2015-08-25T11:00:00Z
modified: 2015-09-14T06:50:31Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2273_
m user id: 26442
owner id: 26442
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Collection of promoters regulated by LldR and LacI.
mutable provenance: Synthetic synthesis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: lldRO2
sequence annotation: lacO
sequence annotation: placUV5
sequence annotation: lldRO1
sequence: https://synbiohub.org/public/igem/BBa_K1847011_sequence/1

lldRO2
type: SequenceAnnotation
persistent identity: annotation2438093
display id: annotation2438093
version: 1
top level: BBa_K1847011
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K1847011/annotation2438093/range2438093/1

https://synbiohub.org/public/igem/BBa_K1847011/annotation2438093/range2438093/1
type: Range
persistent identity: range2438093
display id: range2438093
version: 1
direction: direction/forward
top level: BBa_K1847011
start: 132
end: 148
orientation: inline

lacO
type: SequenceAnnotation
persistent identity: annotation2438092
display id: annotation2438092
version: 1
top level: BBa_K1847011
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K1847011/annotation2438092/range2438092/1

https://synbiohub.org/public/igem/BBa_K1847011/annotation2438092/range2438092/1
type: Range
persistent identity: range2438092
display id: range2438092
version: 1
direction: direction/forward
top level: BBa_K1847011
start: 110
end: 126
orientation: inline

placUV5
type: SequenceAnnotation
persistent identity: annotation2438091
display id: annotation2438091
version: 1
top level: BBa_K1847011
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1847011/annotation2438091/range2438091/1

https://synbiohub.org/public/igem/BBa_K1847011/annotation2438091/range2438091/1
type: Range
persistent identity: range2438091
display id: range2438091
version: 1
direction: direction/forward
top level: BBa_K1847011
start: 72
end: 102
orientation: inline

lldRO1
type: SequenceAnnotation
persistent identity: annotation2438090
display id: annotation2438090
version: 1
top level: BBa_K1847011
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K1847011/annotation2438090/range2438090/1

https://synbiohub.org/public/igem/BBa_K1847011/annotation2438090/range2438090/1
type: Range
persistent identity: range2438090
display id: range2438090
version: 1
direction: direction/forward
top level: BBa_K1847011
start: 1
end: 17
orientation: inline

BBa_J04450
type: ComponentDefinition
persistent identity: BBa J04450
display id: BBa_J04450
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RFP Coding Device
creator: Tamar Odle
created: 2005-06-08T11:00:00Z
modified: 2015-08-31T04:08:14Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _16_
m user id: 0
owner id: 328
owning group id: 16
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Contains an IPTG inducible promoter an RBS, RFP, and terminator.
mutable provenance: Davidson Synth-Aces
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0034
component: BBa_E1010
component: BBa_R0010
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_R0010
sequence annotation: BBa_E1010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_J04450_sequence/1

BBa_B0034
type: Component
persistent identity: component1509404
display id: component1509404
version: 1
top level: BBa_J04450
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_E1010
type: Component
persistent identity: component1509411
display id: component1509411
version: 1
top level: BBa_J04450
access: public
definition: https://synbiohub.org/public/igem/BBa_E1010/1

BBa_R0010
type: Component
persistent identity: component1509394
display id: component1509394
version: 1
top level: BBa_J04450
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_B0012
type: Component
persistent identity: component1509427
display id: component1509427
version: 1
top level: BBa_J04450
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component1509417
display id: component1509417
version: 1
top level: BBa_J04450
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1509427
display id: annotation1509427
version: 1
top level: BBa_J04450
location: https://synbiohub.org/public/igem/BBa_J04450/annotation1509427/range1509427/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J04450/annotation1509427/range1509427/1
type: Range
persistent identity: range1509427
display id: range1509427
version: 1
direction: direction/forward
top level: BBa_J04450
start: 1029
end: 1069
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1509394
display id: annotation1509394
version: 1
top level: BBa_J04450
location: https://synbiohub.org/public/igem/BBa_J04450/annotation1509394/range1509394/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_J04450/annotation1509394/range1509394/1
type: Range
persistent identity: range1509394
display id: range1509394
version: 1
direction: direction/forward
top level: BBa_J04450
start: 1
end: 200
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation1509411
display id: annotation1509411
version: 1
top level: BBa_J04450
location: https://synbiohub.org/public/igem/BBa_J04450/annotation1509411/range1509411/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_J04450/annotation1509411/range1509411/1
type: Range
persistent identity: range1509411
display id: range1509411
version: 1
direction: direction/forward
top level: BBa_J04450
start: 227
end: 907
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1509417
display id: annotation1509417
version: 1
top level: BBa_J04450
location: https://synbiohub.org/public/igem/BBa_J04450/annotation1509417/range1509417/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J04450/annotation1509417/range1509417/1
type: Range
persistent identity: range1509417
display id: range1509417
version: 1
direction: direction/forward
top level: BBa_J04450
start: 941
end: 1020
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1509404
display id: annotation1509404
version: 1
top level: BBa_J04450
location: https://synbiohub.org/public/igem/BBa_J04450/annotation1509404/range1509404/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J04450/annotation1509404/range1509404/1
type: Range
persistent identity: range1509404
display id: range1509404
version: 1
direction: direction/forward
top level: BBa_J04450
start: 209
end: 220
orientation: inline

BBa_K1690005
type: ComponentDefinition
persistent identity: BBa K1690005
display id: BBa_K1690005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pheromone receptor (C. parapsilosis)
creator: Anna Sosnova, Pavel Zach
created: 2015-09-16T11:00:00Z
modified: 2015-09-17T05:35:03Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2108_
m user id: 25090
owner id: 25090
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This is a coding sequence of C. parapsilosis mating pheromone receptor. Cells with this receptor being expressed react to the C. parapsilosis mating pheromone by the activation of the pheromone mating pathway. The coding sequence was codon optimized for the expression in S. cerevisiae. C-terminal was replaced by a C-terminal from C. albicans for more efficient coupling to the mating pathway in S. cerevisiae. Detailed description can be obtained from the [http://2015.igem.org/Team:Czech_Republic/Project/Synthetic_signals_and_receptors#Receptors iGEM 2015 Czech Republic wiki].
mutable notes: The coding sequence was codon optimized for the expression in S. cerevisiae. Kozak sequence was added before the start codon for a better translation. C-terminal was replaced by a C-terminal from C. albicans for more efficient coupling to the mating pathway in S. cerevisiae.
mutable provenance: The whole sequence was synthetized.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: stop
sequence annotation: Kozak
sequence annotation: Receptor
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_K1690005_sequence/1

stop
type: SequenceAnnotation
persistent identity: annotation2464866
display id: annotation2464866
version: 1
top level: BBa_K1690005
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1690005/annotation2464866/range2464866/1

https://synbiohub.org/public/igem/BBa_K1690005/annotation2464866/range2464866/1
type: Range
persistent identity: range2464866
display id: range2464866
version: 1
direction: direction/forward
top level: BBa_K1690005
start: 1207
end: 1209
orientation: inline

Kozak
type: SequenceAnnotation
persistent identity: annotation2464863
display id: annotation2464863
version: 1
top level: BBa_K1690005
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1690005/annotation2464863/range2464863/1

https://synbiohub.org/public/igem/BBa_K1690005/annotation2464863/range2464863/1
type: Range
persistent identity: range2464863
display id: range2464863
version: 1
direction: direction/forward
top level: BBa_K1690005
start: 1
end: 6
orientation: inline

Receptor
type: SequenceAnnotation
persistent identity: annotation2464865
display id: annotation2464865
version: 1
top level: BBa_K1690005
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1690005/annotation2464865/range2464865/1

https://synbiohub.org/public/igem/BBa_K1690005/annotation2464865/range2464865/1
type: Range
persistent identity: range2464865
display id: range2464865
version: 1
direction: direction/forward
top level: BBa_K1690005
start: 10
end: 1206
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2464864
display id: annotation2464864
version: 1
top level: BBa_K1690005
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1690005/annotation2464864/range2464864/1

https://synbiohub.org/public/igem/BBa_K1690005/annotation2464864/range2464864/1
type: Range
persistent identity: range2464864
display id: range2464864
version: 1
direction: direction/forward
top level: BBa_K1690005
start: 7
end: 9
orientation: inline

Si-tag1+3
type: ComponentDefinition
persistent identity: BBa K1592012
display id: BBa_K1592012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LIP prepro + E. coli ribosomal protein L2 (1-60,203-273aa) + YLcwp3 Fusion
creator: Shuyan Tang
created: 2015-09-06T11:00:00Z
modified: 2015-09-18T09:35:56Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2009_
m user id: 20267
owner id: 20267
owning group id: 9
status: status/Planning
bookmark: true
mutable description: __NOTOC__ BBa_K1592008 short This is the cell display system of Yarrowia lipolytica, composed of LIP prepro, interest protein, and YLcwp3.LIP prepro is signal peptide used to secrete the interest protein out of the cell, and the YLcwp3 is the anchor domain binding the interest protein to the cell wall of yeast.We use this system to display silica-tag and test its binding characteristics. Here we use this system to display Silica-tag on cell wall of Yarrowia lipolytica to binding the silica. E.coli ribosomal protein L2 was found to bind tightly to silicon particles, which have surfaces that are oxidized to silica. This L2 silica-binding tag, called the 'Si-tag', can be used for one-step targeting of functional proteins on silica surfaces. The silica-binding domains of E. coli L2 was mapped to amino acids 1???60, 61-202 and 203???273, called Si-tag1, Si-tag2 and Si-tag3. We respectively test the silica-binding characteristics of this three regions and their combinations.
mutable notes: In order to replace the domains more conveniently, we respectivey added BamHI, SalI, NdeI between LIP2 prepro, E. coli ribosomal protein L2, GS linker and YLcwp3
mutable provenance: E. coli ribosomal protein L2 was synthesized by IDT, LIP2 prepro and YLcwp3 were cloning from the plasmid JMP62, and we fused this three sequences.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: GS Linker
sequence annotation: YLcwp3
sequence annotation: LIP2 prepro
sequence annotation: Si-tag
sequence annotation: BamHI
sequence annotation: NdeI
sequence annotation: SalI
sequence: https://synbiohub.org/public/igem/BBa_K1592012_sequence/1

GS Linker
type: SequenceAnnotation
persistent identity: annotation2456162
display id: annotation2456162
version: 1
top level: Si-tag1+3
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1592012/annotation2456162/range2456162/1

https://synbiohub.org/public/igem/BBa_K1592012/annotation2456162/range2456162/1
type: Range
persistent identity: range2456162
display id: range2456162
version: 1
direction: direction/forward
top level: Si-tag1+3
start: 508
end: 552
orientation: inline

YLcwp3
type: SequenceAnnotation
persistent identity: annotation2456164
display id: annotation2456164
version: 1
top level: Si-tag1+3
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1592012/annotation2456164/range2456164/1

https://synbiohub.org/public/igem/BBa_K1592012/annotation2456164/range2456164/1
type: Range
persistent identity: range2456164
display id: range2456164
version: 1
direction: direction/forward
top level: Si-tag1+3
start: 559
end: 924
orientation: inline

LIP2 prepro
type: SequenceAnnotation
persistent identity: annotation2456158
display id: annotation2456158
version: 1
top level: Si-tag1+3
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1592012/annotation2456158/range2456158/1

https://synbiohub.org/public/igem/BBa_K1592012/annotation2456158/range2456158/1
type: Range
persistent identity: range2456158
display id: range2456158
version: 1
direction: direction/forward
top level: Si-tag1+3
start: 1
end: 99
orientation: inline

Si-tag
type: SequenceAnnotation
persistent identity: annotation2456160
display id: annotation2456160
version: 1
top level: Si-tag1+3
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1592012/annotation2456160/range2456160/1

https://synbiohub.org/public/igem/BBa_K1592012/annotation2456160/range2456160/1
type: Range
persistent identity: range2456160
display id: range2456160
version: 1
direction: direction/forward
top level: Si-tag1+3
start: 106
end: 501
orientation: inline

BamHI
type: SequenceAnnotation
persistent identity: annotation2456159
display id: annotation2456159
version: 1
top level: Si-tag1+3
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1592012/annotation2456159/range2456159/1

https://synbiohub.org/public/igem/BBa_K1592012/annotation2456159/range2456159/1
type: Range
persistent identity: range2456159
display id: range2456159
version: 1
direction: direction/forward
top level: Si-tag1+3
start: 100
end: 105
orientation: inline

NdeI
type: SequenceAnnotation
persistent identity: annotation2456163
display id: annotation2456163
version: 1
top level: Si-tag1+3
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1592012/annotation2456163/range2456163/1

https://synbiohub.org/public/igem/BBa_K1592012/annotation2456163/range2456163/1
type: Range
persistent identity: range2456163
display id: range2456163
version: 1
direction: direction/forward
top level: Si-tag1+3
start: 553
end: 558
orientation: inline

SalI
type: SequenceAnnotation
persistent identity: annotation2456161
display id: annotation2456161
version: 1
top level: Si-tag1+3
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1592012/annotation2456161/range2456161/1

https://synbiohub.org/public/igem/BBa_K1592012/annotation2456161/range2456161/1
type: Range
persistent identity: range2456161
display id: range2456161
version: 1
direction: direction/forward
top level: Si-tag1+3
start: 502
end: 507
orientation: inline

BBa_K1795006
type: ComponentDefinition
persistent identity: BBa K1795006
display id: BBa_K1795006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23100 gRNA under R0040
creator: Andrew D Halleran, Joseph L Maniaci
created: 2015-09-16T11:00:00Z
modified: 2015-09-17T09:42:20Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2220_
m user id: 27446
owner id: 27446
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This sgRNA sequence was designed to target the Promoter Bba_J23100 with the N20 sequence ttgacggctagctcagtcct . If this gRNA is expressed in a cell with a form of the dCas9 protein, the protein will be targeted to the N20 sequence and repress gene expression. The part is under the control of the promoter Bba_R0040. Promoter Bba_R0040 was chosen because it does not contain a PAM and therefore cannot be inadvertently repressed by dCas9 due to unintentional homology in the N20 sequence. Expression of this part can be repressed if TetR is expressed in the cell without Tetracycline present. If TetR and Tetracycline (or its analog aTc) are both present, the part will be expressed.
mutable notes: We made sure to use a different promoter for this part than the one which the part targets.
mutable provenance: The basic template for the sgRNA sequence was taken from the supplementary information of Qi LS, Larson MH, Gilbert LA, et al. Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression. Cell. 2013;152(5):1173-1183. doi:10.1016/j.cell.2013.02.022.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_R0040
component: BBa_K1795106
component: BBa_K1795100
component: BBa_B0015
sequence annotation: BBa_K1795100
sequence annotation: BBa_R0040
sequence annotation: BBa_K1795106
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K1795006_sequence/1

BBa_R0040
type: Component
persistent identity: component2467768
display id: component2467768
version: 1
top level: BBa_K1795006
access: public
definition: p(tetR)

BBa_K1795106
type: Component
persistent identity: component2467773
display id: component2467773
version: 1
top level: BBa_K1795006
access: public
definition: https://synbiohub.org/public/igem/BBa_K1795106/1

BBa_K1795100
type: Component
persistent identity: component2467774
display id: component2467774
version: 1
top level: BBa_K1795006
access: public
definition: https://synbiohub.org/public/igem/BBa_K1795100/1

BBa_B0015
type: Component
persistent identity: component2467781
display id: component2467781
version: 1
top level: BBa_K1795006
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_K1795100
type: SequenceAnnotation
persistent identity: annotation2467774
display id: annotation2467774
version: 1
top level: BBa_K1795006
location: https://synbiohub.org/public/igem/BBa_K1795006/annotation2467774/range2467774/1
component: BBa_K1795100

https://synbiohub.org/public/igem/BBa_K1795006/annotation2467774/range2467774/1
type: Range
persistent identity: range2467774
display id: range2467774
version: 1
direction: direction/reverse
top level: BBa_K1795006
start: 75
end: 156
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2467768
display id: annotation2467768
version: 1
top level: BBa_K1795006
location: https://synbiohub.org/public/igem/BBa_K1795006/annotation2467768/range2467768/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K1795006/annotation2467768/range2467768/1
type: Range
persistent identity: range2467768
display id: range2467768
version: 1
direction: direction/forward
top level: BBa_K1795006
start: 1
end: 54
orientation: inline

BBa_K1795106
type: SequenceAnnotation
persistent identity: annotation2467773
display id: annotation2467773
version: 1
top level: BBa_K1795006
location: https://synbiohub.org/public/igem/BBa_K1795006/annotation2467773/range2467773/1
component: BBa_K1795106

https://synbiohub.org/public/igem/BBa_K1795006/annotation2467773/range2467773/1
type: Range
persistent identity: range2467773
display id: range2467773
version: 1
direction: direction/reverse
top level: BBa_K1795006
start: 55
end: 74
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2467781
display id: annotation2467781
version: 1
top level: BBa_K1795006
location: https://synbiohub.org/public/igem/BBa_K1795006/annotation2467781/range2467781/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1795006/annotation2467781/range2467781/1
type: Range
persistent identity: range2467781
display id: range2467781
version: 1
direction: direction/reverse
top level: BBa_K1795006
start: 157
end: 285
orientation: inline

BBa_K1680009
type: ComponentDefinition
persistent identity: BBa K1680009
display id: BBa_K1680009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NanoLuc Luciferase
creator: Katharina Sporbeck
created: 2015-09-16T11:00:00Z
modified: 2015-10-27T01:37:07Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2098_
m user id: 15693
owner id: 26460
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Protein coding region for the NanoLuc(tm) Luziferase (designed by the Promega Corporation, free for research use), Codon optimized for yeast. The Nanoluc is smaller than other luziferase proteins and yields equal or stronger luminescence readings.
mutable notes: Part is shipped in RFC10.
mutable provenance: gBlock designed by Team Tuebingen, sequence by Promega
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: NanoLuc
sequence: https://synbiohub.org/public/igem/BBa_K1680009_sequence/1

NanoLuc
type: SequenceAnnotation
persistent identity: annotation2477413
display id: annotation2477413
version: 1
top level: BBa_K1680009
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680009/annotation2477413/range2477413/1

https://synbiohub.org/public/igem/BBa_K1680009/annotation2477413/range2477413/1
type: Range
persistent identity: range2477413
display id: range2477413
version: 1
direction: direction/forward
top level: BBa_K1680009
start: 1
end: 516
orientation: inline

BBa_K1763012
type: ComponentDefinition
persistent identity: BBa K1763012
display id: BBa_K1763012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Major Spidroin Protein 1 (MaSp1) with Sticky Ends CA
creator: Vinson Lam
created: 2015-07-26T11:00:00Z
modified: 2015-09-18T11:29:29Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2185_
m user id: 27136
owner id: 27136
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This part contains the core sequence of MaSp1 that has been assembled with a 5'-AGTT-3' overhang on the 5' end of the sequence and 3'-ACAG-5' overhang on the 3' end of the sequence. This part was designed for use with ICA, along with parts BBa_K1763003 and BBa_K1763004 as an efficient method to assemble multiple MaSp sequences together. This setup is to show that this particular set of sticky ends is suitable for ICA. Before using in ICA, this part should be digested with BsaI, a type IIs restriction enzyme, which cuts DNA outside of its recognition site.
mutable notes: The gene sequence for MaSp2 is particularly GC-rich, which may complicate PCR.
mutable provenance: This part is derived from the MaSp2 gene found in the spider N. Clavipes.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: BsaI recognition site (forward)
sequence annotation: BsaI recognition site (reverse)
sequence annotation: C sticky end
sequence annotation: A sticky end
sequence annotation: MaSp1 Core
sequence: https://synbiohub.org/public/igem/BBa_K1763012_sequence/1

BsaI recognition site (forward)
type: SequenceAnnotation
persistent identity: annotation2433556
display id: annotation2433556
version: 1
top level: BBa_K1763012
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1763012/annotation2433556/range2433556/1

https://synbiohub.org/public/igem/BBa_K1763012/annotation2433556/range2433556/1
type: Range
persistent identity: range2433556
display id: range2433556
version: 1
direction: direction/forward
top level: BBa_K1763012
start: 1
end: 5
orientation: inline

BsaI recognition site (reverse)
type: SequenceAnnotation
persistent identity: annotation2433557
display id: annotation2433557
version: 1
top level: BBa_K1763012
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1763012/annotation2433557/range2433557/1

https://synbiohub.org/public/igem/BBa_K1763012/annotation2433557/range2433557/1
type: Range
persistent identity: range2433557
display id: range2433557
version: 1
direction: direction/reverse
top level: BBa_K1763012
start: 114
end: 119
orientation: inline

C sticky end
type: SequenceAnnotation
persistent identity: annotation2463777
display id: annotation2463777
version: 1
top level: BBa_K1763012
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1763012/annotation2463777/range2463777/1

https://synbiohub.org/public/igem/BBa_K1763012/annotation2463777/range2463777/1
type: Range
persistent identity: range2463777
display id: range2463777
version: 1
direction: direction/forward
top level: BBa_K1763012
start: 7
end: 10
orientation: inline

A sticky end
type: SequenceAnnotation
persistent identity: annotation2463778
display id: annotation2463778
version: 1
top level: BBa_K1763012
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1763012/annotation2463778/range2463778/1

https://synbiohub.org/public/igem/BBa_K1763012/annotation2463778/range2463778/1
type: Range
persistent identity: range2463778
display id: range2463778
version: 1
direction: direction/forward
top level: BBa_K1763012
start: 109
end: 112
orientation: inline

MaSp1 Core
type: SequenceAnnotation
persistent identity: annotation2473425
display id: annotation2473425
version: 1
top level: BBa_K1763012
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763012/annotation2473425/range2473425/1

https://synbiohub.org/public/igem/BBa_K1763012/annotation2473425/range2473425/1
type: Range
persistent identity: range2473425
display id: range2473425
version: 1
direction: direction/forward
top level: BBa_K1763012
start: 11
end: 108
orientation: inline

BBa_K1763007
type: ComponentDefinition
persistent identity: BBa K1763007
display id: BBa_K1763007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Honeybee silk protein driven by T7 promoter
creator: Daniel Cancilla
created: 2015-07-22T11:00:00Z
modified: 2015-07-23T03:43:47Z
discontinued: false
dominant: false
group u list: _2185_
m user id: 18035
owner id: 18035
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This part is the Apis Mellifera (Honeybee) silk protein coding region, with a t7 promoter element upstream as well as a ribosomal binding site.
mutable notes: Keep in mind that in order for the promoter to work as expected, the plasmid must be transformed into a cell line that contains a gene coding for T7 polymerase.
mutable provenance: Genbank FJ235090.1 Genomic sequence from Apis Mellifera
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K525998
component: BBa_K1763000
sequence annotation: BBa_K525998
sequence annotation: BBa_K1763000
sequence: https://synbiohub.org/public/igem/BBa_K1763007_sequence/1

BBa_K525998
type: Component
persistent identity: component2433354
display id: component2433354
version: 1
top level: BBa_K1763007
access: public
definition: https://synbiohub.org/public/igem/BBa_K525998/1

BBa_K1763000
type: Component
persistent identity: component2433357
display id: component2433357
version: 1
top level: BBa_K1763007
access: public
definition: BBa_K1763000

BBa_K525998
type: SequenceAnnotation
persistent identity: annotation2433354
display id: annotation2433354
version: 1
top level: BBa_K1763007
location: https://synbiohub.org/public/igem/BBa_K1763007/annotation2433354/range2433354/1
component: BBa_K525998

https://synbiohub.org/public/igem/BBa_K1763007/annotation2433354/range2433354/1
type: Range
persistent identity: range2433354
display id: range2433354
version: 1
direction: direction/reverse
top level: BBa_K1763007
start: 1
end: 32
orientation: inline

BBa_K1763000
type: SequenceAnnotation
persistent identity: annotation2433357
display id: annotation2433357
version: 1
top level: BBa_K1763007
location: https://synbiohub.org/public/igem/BBa_K1763007/annotation2433357/range2433357/1
component: BBa_K1763000

https://synbiohub.org/public/igem/BBa_K1763007/annotation2433357/range2433357/1
type: Range
persistent identity: range2433357
display id: range2433357
version: 1
direction: direction/reverse
top level: BBa_K1763007
start: 39
end: 989
orientation: inline

BBa_K1692001
type: ComponentDefinition
persistent identity: BBa K1692001
display id: BBa_K1692001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ferulic acid decarboxylase (FDC) with T7 promoter and RBS
creator: Daniel Kunin
created: 2015-08-02T11:00:00Z
modified: 2015-08-03T12:51:21Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2110_
m user id: 26209
owner id: 26209
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Ferulic acid decarboxylase (FDC) with T7 promoter and RBS
mutable notes: Site directed mutagenesis of SpeI restriction enzyme site within sequence.
mutable provenance: Saccharomyces cerevisiae
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1692000
component: BBa_K525998
sequence annotation: BBa_K1692000
sequence annotation: BBa_K525998
sequence: https://synbiohub.org/public/igem/BBa_K1692001_sequence/1

BBa_K1692000
type: Component
persistent identity: component2433977
display id: component2433977
version: 1
top level: BBa_K1692001
access: public
definition: https://synbiohub.org/public/igem/BBa_K1692000/1

BBa_K525998
type: Component
persistent identity: component2433976
display id: component2433976
version: 1
top level: BBa_K1692001
access: public
definition: https://synbiohub.org/public/igem/BBa_K525998/1

BBa_K1692000
type: SequenceAnnotation
persistent identity: annotation2433977
display id: annotation2433977
version: 1
top level: BBa_K1692001
location: https://synbiohub.org/public/igem/BBa_K1692001/annotation2433977/range2433977/1
component: BBa_K1692000

https://synbiohub.org/public/igem/BBa_K1692001/annotation2433977/range2433977/1
type: Range
persistent identity: range2433977
display id: range2433977
version: 1
direction: direction/reverse
top level: BBa_K1692001
start: 39
end: 1550
orientation: inline

BBa_K525998
type: SequenceAnnotation
persistent identity: annotation2433976
display id: annotation2433976
version: 1
top level: BBa_K1692001
location: https://synbiohub.org/public/igem/BBa_K1692001/annotation2433976/range2433976/1
component: BBa_K525998

https://synbiohub.org/public/igem/BBa_K1692001/annotation2433976/range2433976/1
type: Range
persistent identity: range2433976
display id: range2433976
version: 1
direction: direction/reverse
top level: BBa_K1692001
start: 1
end: 32
orientation: inline

BBa_K1595001
type: ComponentDefinition
persistent identity: BBa K1595001
display id: BBa_K1595001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: EcnB from Methyloversatilis universalis
creator: Jonlin Chen
created: 2015-09-04T11:00:00Z
modified: 2015-09-05T02:52:24Z
discontinued: false
dominant: false
group u list: _2012_
m user id: 18839
owner id: 18839
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Coding device for anti-microbial peptide derived from Methyloversatilis universalis EcnB.
mutable notes: Use in conjunction with strain-expressing T7 polymerase.
mutable provenance: Methyloversatilis universalis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Terminator
sequence annotation: EcnB
sequence annotation: 6xHist tag
sequence annotation: RBS
sequence annotation: T7
sequence: https://synbiohub.org/public/igem/BBa_K1595001_sequence/1

Terminator
type: SequenceAnnotation
persistent identity: annotation2443812
display id: annotation2443812
version: 1
top level: BBa_K1595001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1595001/annotation2443812/range2443812/1

https://synbiohub.org/public/igem/BBa_K1595001/annotation2443812/range2443812/1
type: Range
persistent identity: range2443812
display id: range2443812
version: 1
direction: direction/forward
top level: BBa_K1595001
start: 222
end: 260
orientation: inline

EcnB
type: SequenceAnnotation
persistent identity: annotation2443810
display id: annotation2443810
version: 1
top level: BBa_K1595001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1595001/annotation2443810/range2443810/1

https://synbiohub.org/public/igem/BBa_K1595001/annotation2443810/range2443810/1
type: Range
persistent identity: range2443810
display id: range2443810
version: 1
direction: direction/forward
top level: BBa_K1595001
start: 81
end: 200
orientation: inline

6xHist tag
type: SequenceAnnotation
persistent identity: annotation2443811
display id: annotation2443811
version: 1
top level: BBa_K1595001
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1595001/annotation2443811/range2443811/1

https://synbiohub.org/public/igem/BBa_K1595001/annotation2443811/range2443811/1
type: Range
persistent identity: range2443811
display id: range2443811
version: 1
direction: direction/forward
top level: BBa_K1595001
start: 201
end: 218
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2443809
display id: annotation2443809
version: 1
top level: BBa_K1595001
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1595001/annotation2443809/range2443809/1

https://synbiohub.org/public/igem/BBa_K1595001/annotation2443809/range2443809/1
type: Range
persistent identity: range2443809
display id: range2443809
version: 1
direction: direction/forward
top level: BBa_K1595001
start: 22
end: 80
orientation: inline

T7
type: SequenceAnnotation
persistent identity: annotation2443808
display id: annotation2443808
version: 1
top level: BBa_K1595001
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1595001/annotation2443808/range2443808/1

https://synbiohub.org/public/igem/BBa_K1595001/annotation2443808/range2443808/1
type: Range
persistent identity: range2443808
display id: range2443808
version: 1
direction: direction/forward
top level: BBa_K1595001
start: 2
end: 20
orientation: inline

BBa_K1847002
type: ComponentDefinition
persistent identity: BBa K1847002
display id: BBa_K1847002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23100-lldRO1-lldRO2
creator: Anna Fomitcheva, Lisa Baumgartner
created: 2015-08-25T11:00:00Z
modified: 2015-09-16T02:33:25Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _2273_
m user id: 26442
owner id: 26442
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Collection of promoters regulated by LldR
mutable provenance: Synthetic synthesis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: lldRO1
sequence annotation: J23100
sequence annotation: lldRO2
sequence: https://synbiohub.org/public/igem/BBa_K1847002_sequence/1

lldRO1
type: SequenceAnnotation
persistent identity: annotation2438049
display id: annotation2438049
version: 1
top level: BBa_K1847002
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K1847002/annotation2438049/range2438049/1

https://synbiohub.org/public/igem/BBa_K1847002/annotation2438049/range2438049/1
type: Range
persistent identity: range2438049
display id: range2438049
version: 1
direction: direction/forward
top level: BBa_K1847002
start: 44
end: 60
orientation: inline

J23100
type: SequenceAnnotation
persistent identity: annotation2438048
display id: annotation2438048
version: 1
top level: BBa_K1847002
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1847002/annotation2438048/range2438048/1

https://synbiohub.org/public/igem/BBa_K1847002/annotation2438048/range2438048/1
type: Range
persistent identity: range2438048
display id: range2438048
version: 1
direction: direction/forward
top level: BBa_K1847002
start: 1
end: 35
orientation: inline

lldRO2
type: SequenceAnnotation
persistent identity: annotation2438050
display id: annotation2438050
version: 1
top level: BBa_K1847002
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K1847002/annotation2438050/range2438050/1

https://synbiohub.org/public/igem/BBa_K1847002/annotation2438050/range2438050/1
type: Range
persistent identity: range2438050
display id: range2438050
version: 1
direction: direction/forward
top level: BBa_K1847002
start: 61
end: 77
orientation: inline

BBa_K1763421
type: ComponentDefinition
persistent identity: BBa K1763421
display id: BBa_K1763421
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PCquad Mutant 10
creator: Tyler Lee
created: 2015-09-10T11:00:00Z
modified: 2016-10-02T06:33:24Z
discontinued: false
dominant: false
group u list: _2185_
m user id: 27134
owner id: 27528
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Subunit of a 12-subunit protein cage with a thrombin cleavage sequence inserted between residues 243 and 248 (ALPSAE to ALPGLVPRGSGSAE).
mutable notes: Considered the area to insert the cleavage sequence. This mutation was selected by examining the secondary structure to ensure minimal disruption of existing alpha-helices or beta sheets, sites were sandwiched by glycine residues, and used PyMOL to check if the site would be accessible to the protease. Geometric accessibility by the protease thrombin and minimal speculated extent of conformational changes by insertion.
mutable provenance: Bromoperoxidase (trimer) and M1 Virus Matrix Protein (dimer) linked with an alpha helix.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: Start
sequence annotation: RBS
sequence annotation: T7 Promoter
sequence annotation: Double Stop
sequence annotation: Scar
sequence annotation: PCquad Mutant 10
sequence annotation: 6x His Tag
sequence: https://synbiohub.org/public/igem/BBa_K1763421_sequence/1

Start
type: SequenceAnnotation
persistent identity: annotation2467612
display id: annotation2467612
version: 1
top level: BBa_K1763421
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1763421/annotation2467612/range2467612/1

https://synbiohub.org/public/igem/BBa_K1763421/annotation2467612/range2467612/1
type: Range
persistent identity: range2467612
display id: range2467612
version: 1
direction: direction/forward
top level: BBa_K1763421
start: 40
end: 42
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2467609
display id: annotation2467609
version: 1
top level: BBa_K1763421
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1763421/annotation2467609/range2467609/1

https://synbiohub.org/public/igem/BBa_K1763421/annotation2467609/range2467609/1
type: Range
persistent identity: range2467609
display id: range2467609
version: 1
direction: direction/forward
top level: BBa_K1763421
start: 24
end: 28
orientation: inline

T7 Promoter
type: SequenceAnnotation
persistent identity: annotation2467608
display id: annotation2467608
version: 1
top level: BBa_K1763421
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1763421/annotation2467608/range2467608/1

https://synbiohub.org/public/igem/BBa_K1763421/annotation2467608/range2467608/1
type: Range
persistent identity: range2467608
display id: range2467608
version: 1
direction: direction/forward
top level: BBa_K1763421
start: 1
end: 22
orientation: inline

Double Stop
type: SequenceAnnotation
persistent identity: annotation2467611
display id: annotation2467611
version: 1
top level: BBa_K1763421
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1763421/annotation2467611/range2467611/1

https://synbiohub.org/public/igem/BBa_K1763421/annotation2467611/range2467611/1
type: Range
persistent identity: range2467611
display id: range2467611
version: 1
direction: direction/forward
top level: BBa_K1763421
start: 1425
end: 1430
orientation: inline

Scar
type: SequenceAnnotation
persistent identity: annotation2467610
display id: annotation2467610
version: 1
top level: BBa_K1763421
role: feature/scar
location: https://synbiohub.org/public/igem/BBa_K1763421/annotation2467610/range2467610/1

https://synbiohub.org/public/igem/BBa_K1763421/annotation2467610/range2467610/1
type: Range
persistent identity: range2467610
display id: range2467610
version: 1
direction: direction/forward
top level: BBa_K1763421
start: 34
end: 39
orientation: inline

PCquad Mutant 10
type: SequenceAnnotation
persistent identity: annotation2467630
display id: annotation2467630
version: 1
top level: BBa_K1763421
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1763421/annotation2467630/range2467630/1

https://synbiohub.org/public/igem/BBa_K1763421/annotation2467630/range2467630/1
type: Range
persistent identity: range2467630
display id: range2467630
version: 1
direction: direction/forward
top level: BBa_K1763421
start: 40
end: 1385
orientation: inline

6x His Tag
type: SequenceAnnotation
persistent identity: annotation2467628
display id: annotation2467628
version: 1
top level: BBa_K1763421
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1763421/annotation2467628/range2467628/1

https://synbiohub.org/public/igem/BBa_K1763421/annotation2467628/range2467628/1
type: Range
persistent identity: range2467628
display id: range2467628
version: 1
direction: direction/forward
top level: BBa_K1763421
start: 1386
end: 1400
orientation: inline

BBa_K1632010
type: ComponentDefinition
persistent identity: BBa K1632010
display id: BBa_K1632010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: fimB (wild-type)
creator: Riku Shinohara
created: 2015-08-17T11:00:00Z
modified: 2015-09-17T02:45:06Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2049_
m user id: 24760
owner id: 22687
owning group id: 9
status: status/Planning
bookmark: false
mutable description: a
mutable notes: a
mutable provenance: a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1632010_sequence/1

BBa_K1850010
type: ComponentDefinition
persistent identity: BBa K1850010
display id: BBa_K1850010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pRha - fimH 136 KO - RPMrel - SpyTag_225 - HisTag_225
creator: Lydia Goldberg
created: 2015-09-14T11:00:00Z
modified: 2015-09-16T10:47:41Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2277_
m user id: 26421
owner id: 26421
owning group id: 9
status: status/Planning
bookmark: false
mutable description: placeholder
mutable notes: placeholder
mutable provenance: placeholder
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: fimH
sequence annotation: SpyTag
sequence annotation: pRha
sequence annotation: HisTag
sequence annotation: RPMrel
sequence annotation: B0034
sequence: https://synbiohub.org/public/igem/BBa_K1850010_sequence/1

fimH
type: SequenceAnnotation
persistent identity: annotation2463784
display id: annotation2463784
version: 1
top level: BBa_K1850010
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1850010/annotation2463784/range2463784/1

https://synbiohub.org/public/igem/BBa_K1850010/annotation2463784/range2463784/1
type: Range
persistent identity: range2463784
display id: range2463784
version: 1
direction: direction/forward
top level: BBa_K1850010
start: 270
end: 1256
orientation: inline

SpyTag
type: SequenceAnnotation
persistent identity: annotation2463786
display id: annotation2463786
version: 1
top level: BBa_K1850010
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1850010/annotation2463786/range2463786/1

https://synbiohub.org/public/igem/BBa_K1850010/annotation2463786/range2463786/1
type: Range
persistent identity: range2463786
display id: range2463786
version: 1
direction: direction/forward
top level: BBa_K1850010
start: 1032
end: 1070
orientation: inline

pRha
type: SequenceAnnotation
persistent identity: annotation2463782
display id: annotation2463782
version: 1
top level: BBa_K1850010
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1850010/annotation2463782/range2463782/1

https://synbiohub.org/public/igem/BBa_K1850010/annotation2463782/range2463782/1
type: Range
persistent identity: range2463782
display id: range2463782
version: 1
direction: direction/forward
top level: BBa_K1850010
start: 4
end: 243
orientation: inline

HisTag
type: SequenceAnnotation
persistent identity: annotation2463787
display id: annotation2463787
version: 1
top level: BBa_K1850010
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1850010/annotation2463787/range2463787/1

https://synbiohub.org/public/igem/BBa_K1850010/annotation2463787/range2463787/1
type: Range
persistent identity: range2463787
display id: range2463787
version: 1
direction: direction/forward
top level: BBa_K1850010
start: 1071
end: 1088
orientation: inline

RPMrel
type: SequenceAnnotation
persistent identity: annotation2463785
display id: annotation2463785
version: 1
top level: BBa_K1850010
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1850010/annotation2463785/range2463785/1

https://synbiohub.org/public/igem/BBa_K1850010/annotation2463785/range2463785/1
type: Range
persistent identity: range2463785
display id: range2463785
version: 1
direction: direction/forward
top level: BBa_K1850010
start: 333
end: 359
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2463783
display id: annotation2463783
version: 1
top level: BBa_K1850010
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1850010/annotation2463783/range2463783/1

https://synbiohub.org/public/igem/BBa_K1850010/annotation2463783/range2463783/1
type: Range
persistent identity: range2463783
display id: range2463783
version: 1
direction: direction/forward
top level: BBa_K1850010
start: 252
end: 263
orientation: inline

3alpha-HSD
type: ComponentDefinition
persistent identity: BBa K1674001
display id: BBa_K1674001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 3alpha-hydroxysteroid dehydrogenase
creator: Liron Abrahami
created: 2015-09-12T11:00:00Z
modified: 2015-09-18T02:27:41Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2092_
m user id: 25765
owner id: 25765
owning group id: 9
status: status/Planning
bookmark: true
mutable description: 3α- HSD
mutable notes: k
mutable provenance: gblock ordered from IDT
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1674001_sequence/1

BBa_K1763424
type: ComponentDefinition
persistent identity: BBa K1763424
display id: BBa_K1763424
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: MaSp2-3(1C3)
creator: Vinson Lam
created: 2015-09-14T11:00:00Z
modified: 2015-09-16T11:06:16Z
discontinued: false
dominant: false
group u list: _2185_
m user id: 27136
owner id: 27136
owning group id: 9
status: status/Planning
bookmark: false
mutable description: 3-mer construct of MaSp2 only.
mutable notes: Assembled using ICA
mutable provenance: Nephila clavipes
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: 8-his tag
sequence annotation: M2-CA
sequence annotation: M2-AB
sequence annotation: M2-BC
sequence: https://synbiohub.org/public/igem/BBa_K1763424_sequence/1

8-his tag
type: SequenceAnnotation
persistent identity: annotation2463797
display id: annotation2463797
version: 1
top level: BBa_K1763424
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1763424/annotation2463797/range2463797/1

https://synbiohub.org/public/igem/BBa_K1763424/annotation2463797/range2463797/1
type: Range
persistent identity: range2463797
display id: range2463797
version: 1
direction: direction/forward
top level: BBa_K1763424
start: 4
end: 27
orientation: inline

M2-CA
type: SequenceAnnotation
persistent identity: annotation2463823
display id: annotation2463823
version: 1
top level: BBa_K1763424
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763424/annotation2463823/range2463823/1

https://synbiohub.org/public/igem/BBa_K1763424/annotation2463823/range2463823/1
type: Range
persistent identity: range2463823
display id: range2463823
version: 1
direction: direction/forward
top level: BBa_K1763424
start: 240
end: 341
orientation: inline

M2-AB
type: SequenceAnnotation
persistent identity: annotation2463808
display id: annotation2463808
version: 1
top level: BBa_K1763424
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763424/annotation2463808/range2463808/1

https://synbiohub.org/public/igem/BBa_K1763424/annotation2463808/range2463808/1
type: Range
persistent identity: range2463808
display id: range2463808
version: 1
direction: direction/forward
top level: BBa_K1763424
start: 36
end: 137
orientation: inline

M2-BC
type: SequenceAnnotation
persistent identity: annotation2463818
display id: annotation2463818
version: 1
top level: BBa_K1763424
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763424/annotation2463818/range2463818/1

https://synbiohub.org/public/igem/BBa_K1763424/annotation2463818/range2463818/1
type: Range
persistent identity: range2463818
display id: range2463818
version: 1
direction: direction/forward
top level: BBa_K1763424
start: 138
end: 239
orientation: inline

Fla-Art175
type: ComponentDefinition
persistent identity: BBa K1659001
display id: BBa_K1659001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Artilysin Art-175 fused at N-terminal with flagellin 26-47 peptide segment
creator: Raphaella Hull
created: 2015-08-09T11:00:00Z
modified: 2016-02-01T12:51:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2077_
m user id: 4206
owner id: 24525
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Artilysins are an exciting class of enzyme-based antibacterials. Their name is derived from "artificial endolysin" and they exploit the lytic power of bacteriophage-encoded endolyins. Endolysins are peptidoglycan hydrolases produced at the end of the lytic cycle that pass through the cytoplasmic membrane, degrade the peptidoglycan layer and cause the osmotic lysis of the infected bacterial cell, thus liberating the progeny. Purified endolysins have been used to kill Gram-positive pathogens. Gram-negative bacteria, however, have a protective outer membrane containing lipopolysaccharide (LPS) that serves as a barrier against the peptidoglycan hydolytic activity of endolysins from the outside. To overcome this problem, selected polycationic or amphipathic peptides that locally destabilize the LPS layer have been covalently fused to endolysins. Biers et al. fused the sheep myeloid antimicrobial peptide (SMAP-29) to the N terminus of the endolysin KZ144 to create Artilysin 175. Art-175 has been shown to be a highly potent antibacterial that acts in minutes to kill virtually all Pseudomonas aeruginosa strains. [1] BBa_K1659001 is a composite of Art-175 with the 26-47 segment of Salmonella typhimurium flagellin combined with a hexahistidine tag. [2] Fla 26-47 is capable of mediating flagellar export of recombinant proteins and, combined with a hexahistidine tag, facilitates their secreted expression and easy purification. [1] Briers, Y., Walmagh, M., Grymonprez, B., Biebl, M., Pirnay, J. P., Defraine, V., ??? Lavigne, R. (2014). Art-175 is a highly efficient antibacterial against multidrug-resistant strains and persisters of Pseudomonas aeruginosa. Antimicrobial Agents and Chemotherapy, 58(7), 3774???3784. http://doi.org/10.1128/AAC.02668-14 [2] Balb??s, P., & Lorence, A. (2004). Recombinant Gene Expression. Reviews and Protocols, 267, 506. http://doi.org/10.1007/978-1-61779-433-9
mutable notes: Export signal combined with hexahistidine tag
mutable provenance: Art-175 amino acid sequence: Briers, Y., Walmagh, M., Grymonprez, B., Biebl, M., Pirnay, J. P., Defraine, V., ??? Lavigne, R. (2014). Art-175 is a highly efficient antibacterial against multidrug-resistant strains and persisters of Pseudomonas aeruginosa. Antimicrobial Agents and Chemotherapy, 58(7), 3774???3784. http://doi.org/10.1128/AAC.02668-14 Amino acid sequence of Fla 26-47: Balb??s, P., & Lorence, A. (2004). Recombinant Gene Expression. Reviews and Protocols, 267, 506. http://doi.org/10.1007/978-1-61779-433-9
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: Hisx6
sequence annotation: stop
sequence annotation: start
sequence annotation: Flagellin 26-47 Secretion Signal Tag
sequence annotation: Art-175
sequence: https://synbiohub.org/public/igem/BBa_K1659001_sequence/1

Hisx6
type: SequenceAnnotation
persistent identity: annotation2438070
display id: annotation2438070
version: 1
top level: Fla-Art175
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1659001/annotation2438070/range2438070/1

https://synbiohub.org/public/igem/BBa_K1659001/annotation2438070/range2438070/1
type: Range
persistent identity: range2438070
display id: range2438070
version: 1
direction: direction/forward
top level: Fla-Art175
start: 1033
end: 1050
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2438074
display id: annotation2438074
version: 1
top level: Fla-Art175
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1659001/annotation2438074/range2438074/1

https://synbiohub.org/public/igem/BBa_K1659001/annotation2438074/range2438074/1
type: Range
persistent identity: range2438074
display id: range2438074
version: 1
direction: direction/forward
top level: Fla-Art175
start: 1054
end: 1056
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2438066
display id: annotation2438066
version: 1
top level: Fla-Art175
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1659001/annotation2438066/range2438066/1

https://synbiohub.org/public/igem/BBa_K1659001/annotation2438066/range2438066/1
type: Range
persistent identity: range2438066
display id: range2438066
version: 1
direction: direction/forward
top level: Fla-Art175
start: 1
end: 3
orientation: inline

Flagellin 26-47 Secretion Signal Tag
type: SequenceAnnotation
persistent identity: annotation2438058
display id: annotation2438058
version: 1
top level: Fla-Art175
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1659001/annotation2438058/range2438058/1

https://synbiohub.org/public/igem/BBa_K1659001/annotation2438058/range2438058/1
type: Range
persistent identity: range2438058
display id: range2438058
version: 1
direction: direction/forward
top level: Fla-Art175
start: 4
end: 165
orientation: inline

Art-175
type: SequenceAnnotation
persistent identity: annotation2438068
display id: annotation2438068
version: 1
top level: Fla-Art175
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1659001/annotation2438068/range2438068/1

https://synbiohub.org/public/igem/BBa_K1659001/annotation2438068/range2438068/1
type: Range
persistent identity: range2438068
display id: range2438068
version: 1
direction: direction/forward
top level: Fla-Art175
start: 166
end: 1032
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2438072
display id: annotation2438072
version: 1
top level: Fla-Art175
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1659001/annotation2438072/range2438072/1

https://synbiohub.org/public/igem/BBa_K1659001/annotation2438072/range2438072/1
type: Range
persistent identity: range2438072
display id: range2438072
version: 1
direction: direction/forward
top level: Fla-Art175
start: 1051
end: 1053
orientation: inline

BBa_K1614000
type: ComponentDefinition
persistent identity: BBa K1614000
display id: BBa_K1614000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 promoter for expression of functional RNA
creator: Stefan Holderbach
created: 2015-09-13T11:00:00Z
modified: 2015-09-14T12:29:29Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2031_
m user id: 12380
owner id: 16422
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Test
mutable notes: None.
mutable provenance: T7 phage minimal promoter (consensus)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: T7 promoter
sequence: https://synbiohub.org/public/igem/BBa_K1614000_sequence/1

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2454728
display id: annotation2454728
version: 1
top level: BBa_K1614000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1614000/annotation2454728/range2454728/1

https://synbiohub.org/public/igem/BBa_K1614000/annotation2454728/range2454728/1
type: Range
persistent identity: range2454728
display id: range2454728
version: 1
direction: direction/forward
top level: BBa_K1614000
start: 1
end: 17
orientation: inline

cadA
type: ComponentDefinition
persistent identity: BBa K1602003
display id: BBa_K1602003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cis-aconitate decarboxylase (cadA) from Aspergillus terreus
creator: Alexandra Goretzki, Tanja Habeck, Nina Kuschik-Maczollek, Laurin Monnheimer, Steven Pilger, Bianca Reisinger, Daniel Stumpf, Sebastian Barthel
created: 2015-08-27T11:00:00Z
modified: 2015-09-25T01:18:30Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2019_
m user id: 12512
owner id: 25989
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Blank
mutable notes: Blank
mutable provenance: Aspergillus terreus
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1602003_sequence/1

BBa_K1602016
type: ComponentDefinition
persistent identity: BBa K1602016
display id: BBa_K1602016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0034-xylB-B0034-xylC
creator: Leon Kraus, Jonathan Pletzer-Zelgert, Jonas Sindlinger, Carmen Sakas Gandullo, Sebastian Holzner, Elena Tonchevska, Mirjam Haun
created: 2015-09-15T11:00:00Z
modified: 2015-09-25T01:30:41Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2019_
m user id: 12512
owner id: 22022
owning group id: 9
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0034
component: BBa_K1602010
component: BBa_K1602009
sequence annotation: BBa_B0034
sequence annotation: BBa_K1602009
sequence annotation: BBa_K1602010
sequence: https://synbiohub.org/public/igem/BBa_K1602016_sequence/1

BBa_B0034
type: Component
persistent identity: component2463077
display id: component2463077
version: 1
top level: BBa_K1602016
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: Component
persistent identity: component2463074
display id: component2463074
version: 1
top level: BBa_K1602016
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1602010
type: Component
persistent identity: component2463078
display id: component2463078
version: 1
top level: BBa_K1602016
access: public
definition: BBa_K1602010

BBa_K1602009
type: Component
persistent identity: component2463075
display id: component2463075
version: 1
top level: BBa_K1602016
access: public
definition: BBa_K1602009

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2463077
display id: annotation2463077
version: 1
top level: BBa_K1602016
location: https://synbiohub.org/public/igem/BBa_K1602016/annotation2463077/range2463077/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1602016/annotation2463077/range2463077/1
type: Range
persistent identity: range2463077
display id: range2463077
version: 1
direction: direction/reverse
top level: BBa_K1602016
start: 774
end: 785
orientation: inline

BBa_K1602009
type: SequenceAnnotation
persistent identity: annotation2463075
display id: annotation2463075
version: 1
top level: BBa_K1602016
location: https://synbiohub.org/public/igem/BBa_K1602016/annotation2463075/range2463075/1
component: BBa_K1602009

https://synbiohub.org/public/igem/BBa_K1602016/annotation2463075/range2463075/1
type: Range
persistent identity: range2463075
display id: range2463075
version: 1
direction: direction/reverse
top level: BBa_K1602016
start: 19
end: 765
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2463074
display id: annotation2463074
version: 1
top level: BBa_K1602016
location: https://synbiohub.org/public/igem/BBa_K1602016/annotation2463074/range2463074/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1602016/annotation2463074/range2463074/1
type: Range
persistent identity: range2463074
display id: range2463074
version: 1
direction: direction/reverse
top level: BBa_K1602016
start: 1
end: 12
orientation: inline

BBa_K1602010
type: SequenceAnnotation
persistent identity: annotation2463078
display id: annotation2463078
version: 1
top level: BBa_K1602016
location: https://synbiohub.org/public/igem/BBa_K1602016/annotation2463078/range2463078/1
component: BBa_K1602010

https://synbiohub.org/public/igem/BBa_K1602016/annotation2463078/range2463078/1
type: Range
persistent identity: range2463078
display id: range2463078
version: 1
direction: direction/reverse
top level: BBa_K1602016
start: 792
end: 1661
orientation: inline

BBa_K1689000
type: ComponentDefinition
persistent identity: BBa K1689000
display id: BBa_K1689000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: sgRNA generator
creator: WEI Weijia
created: 2015-09-15T11:00:00Z
modified: 2015-09-26T11:33:57Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2107_
m user id: 4411
owner id: 26464
owning group id: 9
status: status/Planning
bookmark: true
mutable description: including T7 promotor, LacZ a', crRNA (tracrRNA) and T7 terminator.
mutable notes: TACTAGTAGCGGCCGCTGCAGTCCGGCAAAAAAGGGCAAGGTGTCACCACCCTGCCCTTTTTCTTTAAAACCGAAAAGATTACTTCGCGTTATGCAGGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCACAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGCTCGAGGCTTGGATTCTCACCAATAAAAAACGCCCGGCGGCAACCGAGCGTTCTGAACAAATCCAGATGGAGTTCTGAGGTCATTACTGGATCTATCAACAGGAGTCCAAGCGAGCTCGATATCAAATTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCATTAAGCATTCTGCCGACATGGAAGCCATCACAAACGGCATGATGAACCTGAATCGCCAGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAGACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGAAATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCCTGAAAATCTCGATAACTCAAAAAATACGCCCGGTAGTGATCTTATTTCATTATGGTGAAAGTTGGAACCTCTTACGTGCCCGATCAACTCGAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCA…
mutable provenance: E.coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: lacZ promoter
sequence annotation: lacZa
sequence annotation: crRNA
sequence annotation: T7 promoter
sequence annotation: T7 terminator
sequence: https://synbiohub.org/public/igem/BBa_K1689000_sequence/1

lacZ promoter
type: SequenceAnnotation
persistent identity: annotation2471927
display id: annotation2471927
version: 1
top level: BBa_K1689000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1689000/annotation2471927/range2471927/1

https://synbiohub.org/public/igem/BBa_K1689000/annotation2471927/range2471927/1
type: Range
persistent identity: range2471927
display id: range2471927
version: 1
direction: direction/reverse
top level: BBa_K1689000
start: 303
end: 424
orientation: inline

lacZa
type: SequenceAnnotation
persistent identity: annotation2471926
display id: annotation2471926
version: 1
top level: BBa_K1689000
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1689000/annotation2471926/range2471926/1

https://synbiohub.org/public/igem/BBa_K1689000/annotation2471926/range2471926/1
type: Range
persistent identity: range2471926
display id: range2471926
version: 1
direction: direction/reverse
top level: BBa_K1689000
start: 27
end: 302
orientation: inline

crRNA
type: SequenceAnnotation
persistent identity: annotation2471928
display id: annotation2471928
version: 1
top level: BBa_K1689000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1689000/annotation2471928/range2471928/1

https://synbiohub.org/public/igem/BBa_K1689000/annotation2471928/range2471928/1
type: Range
persistent identity: range2471928
display id: range2471928
version: 1
direction: direction/forward
top level: BBa_K1689000
start: 432
end: 511
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2471925
display id: annotation2471925
version: 1
top level: BBa_K1689000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1689000/annotation2471925/range2471925/1

https://synbiohub.org/public/igem/BBa_K1689000/annotation2471925/range2471925/1
type: Range
persistent identity: range2471925
display id: range2471925
version: 1
direction: direction/forward
top level: BBa_K1689000
start: 1
end: 19
orientation: inline

T7 terminator
type: SequenceAnnotation
persistent identity: annotation2471929
display id: annotation2471929
version: 1
top level: BBa_K1689000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1689000/annotation2471929/range2471929/1

https://synbiohub.org/public/igem/BBa_K1689000/annotation2471929/range2471929/1
type: Range
persistent identity: range2471929
display id: range2471929
version: 1
direction: direction/forward
top level: BBa_K1689000
start: 512
end: 557
orientation: inline

BBa_K1758314
type: ComponentDefinition
persistent identity: BBa K1758314
display id: BBa_K1758314
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Chromium responsive promoter under T7-promoter with UTR-sfGFP
creator: Team Bielefeld-CeBiTec 2015
created: 2015-09-07T11:00:00Z
modified: 2016-01-06T03:34:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2180_
m user id: 4206
owner id: 26630
owning group id: 9
status: status/Planning
bookmark: false
mutable description: T7 controlled Chromium responsive promoter with 5??untranslated region and sfGFP for detection
mutable notes: ...
mutable provenance: ...
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_I719005
component: BBa_K1758312
sequence annotation: BBa_K1758312
sequence annotation: BBa_I719005
sequence: https://synbiohub.org/public/igem/BBa_K1758314_sequence/1

BBa_I719005
type: Component
persistent identity: component2474963
display id: component2474963
version: 1
top level: BBa_K1758314
access: public
definition: https://synbiohub.org/public/igem/BBa_I719005/1

BBa_K1758312
type: Component
persistent identity: component2474969
display id: component2474969
version: 1
top level: BBa_K1758314
access: public
definition: BBa_K1758312

BBa_K1758312
type: SequenceAnnotation
persistent identity: annotation2474969
display id: annotation2474969
version: 1
top level: BBa_K1758314
location: https://synbiohub.org/public/igem/BBa_K1758314/annotation2474969/range2474969/1
component: BBa_K1758312

https://synbiohub.org/public/igem/BBa_K1758314/annotation2474969/range2474969/1
type: Range
persistent identity: range2474969
display id: range2474969
version: 1
direction: direction/reverse
top level: BBa_K1758314
start: 32
end: 1104
orientation: inline

BBa_I719005
type: SequenceAnnotation
persistent identity: annotation2474963
display id: annotation2474963
version: 1
top level: BBa_K1758314
location: https://synbiohub.org/public/igem/BBa_K1758314/annotation2474963/range2474963/1
component: BBa_I719005

https://synbiohub.org/public/igem/BBa_K1758314/annotation2474963/range2474963/1
type: Range
persistent identity: range2474963
display id: range2474963
version: 1
direction: direction/reverse
top level: BBa_K1758314
start: 1
end: 23
orientation: inline

BBa_K1602011
type: ComponentDefinition
persistent identity: BBa K1602011
display id: BBa_K1602011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: yagE
creator: Leon Kraus, Jonathan Pletzer-Zelgert, Jonas Sindlinger, Carmen Sakas Gandullo, Sebastian Holzner, Elena Tonchevska, Mirjam Haun
created: 2015-09-13T11:00:00Z
modified: 2015-09-18T06:39:03Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2019_
m user id: 26220
owner id: 25878
owning group id: 9
status: status/Planning
bookmark: false
mutable description: BLANK
mutable notes: BLANK
mutable provenance: BLANK
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1602011_sequence/1

BBa_K1682018
type: ComponentDefinition
persistent identity: BBa K1682018
display id: BBa_K1682018
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PLTet0-1:NarL, PdcuS:sfGFP, J23109:TetR
creator: Nikola Dyulgyarov
created: 2015-09-16T11:00:00Z
modified: 2015-09-17T09:32:14Z
discontinued: false
dominant: false
group u list: _2100_
m user id: 28169
owner id: 28169
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Functional nitrate anion sensor based on the NarX/NarL Two-Component Signal Transduction System in E. coli with semi-tunable dynamic range and sensitivity. NarL expression is regulated by the TetR-PLTet0-1 1-component regulatory system, whereby addition of ATC (anhydrotetracycline) induces higher NarL expression. This regulates the ratio of native NarX/NarL protein in the cell, controlling to an extent the dynamic range and sensitivities of the sensor. Phosphorylated NarL binds to the repressible PdcuS promoter from E. coli, regulating the expression of sfGFP. Low nitrate conditions result in high sfGFP expression, high nitrate conditions repress transcription. The sensitivity of the sensor is in the sub-mM to mM range for nitrate. Further testing is needed to fully characterize the relationship of ATC-induction to nitrate sensitivity and dynamic range. The sensor is functional in the absence of ATC with a low dynamic range, and ATC concentrations of 1-10 ng/ml increase dynamic range and sensitivity. Further increase of ATC drives the ON-state down, decreasing dynamic range.
mutable notes: The PdcuS promoter was chosen for its NarL binding site, repressible activity, and the absence of other protein-binding domains, suggesting a robust, orthogonal response. The construct was assembled using Golden Gate Assembly.
mutable provenance: NarL, PdcuS - E. coli MG1655 genome sfGFP,TetR-PLTet0-1 scaffold - pRS334 (courtesy of Tabor Lab, Rice Univeristy)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: sRBS TIR900
sequence annotation: NarL CDS
sequence annotation: PLTet0-1
sequence: https://synbiohub.org/public/igem/BBa_K1682018_sequence/1

sRBS TIR900
type: SequenceAnnotation
persistent identity: annotation2466134
display id: annotation2466134
version: 1
top level: BBa_K1682018
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1682018/annotation2466134/range2466134/1

https://synbiohub.org/public/igem/BBa_K1682018/annotation2466134/range2466134/1
type: Range
persistent identity: range2466134
display id: range2466134
version: 1
direction: direction/forward
top level: BBa_K1682018
start: 111
end: 144
orientation: inline

NarL CDS
type: SequenceAnnotation
persistent identity: annotation2466136
display id: annotation2466136
version: 1
top level: BBa_K1682018
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1682018/annotation2466136/range2466136/1

https://synbiohub.org/public/igem/BBa_K1682018/annotation2466136/range2466136/1
type: Range
persistent identity: range2466136
display id: range2466136
version: 1
direction: direction/forward
top level: BBa_K1682018
start: 145
end: 795
orientation: inline

PLTet0-1
type: SequenceAnnotation
persistent identity: annotation2466132
display id: annotation2466132
version: 1
top level: BBa_K1682018
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1682018/annotation2466132/range2466132/1

https://synbiohub.org/public/igem/BBa_K1682018/annotation2466132/range2466132/1
type: Range
persistent identity: range2466132
display id: range2466132
version: 1
direction: direction/forward
top level: BBa_K1682018
start: 37
end: 74
orientation: inline

BBa_K1799008
type: ComponentDefinition
persistent identity: BBa K1799008
display id: BBa_K1799008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LsrC (modularization optimized)
creator: Michael Flanagan
created: 2015-08-06T11:00:00Z
modified: 2015-09-18T05:16:06Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2224_
m user id: 26720
owner id: 19613
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Gene for the C subunit of the LsrACDB transporter, present in E. coli on the LsrACDBFG operon. The C subunit is a permease protein in the transporter, which actively brings autoinducer 2 (AI-2) into the cell. The portion of the LsrACDBFG operon that codes for the AI-2 transporter consists of four open reading frames, which code for A, C, D, and B, respectively. Three of these ORFs overlap with one another: C overlaps with A, and its RBS is contained within the A subunit ORF, and D overlaps with C (although to a lesser extent), and its RBS is contained within the C subunit ORF. From a modularization standpoint, this creates an inefficiency because the wild type ORFs of the A and C subunits both contain functional RBSs, and in the case of the A subunit ORF, an ATG 4bp downstream. To reduce this inefficiency, we have created versions of each subunit ORF that are optimized for modularization.
mutable notes: This version of the open reading frame has been optimized for modularization via silent mutations of the LsrD RBS, as well as any segments of code sharing more than 83% homology with the Shine-Dalgarno sequence. It is otherwise identical to the wild type LsrC gene (http://parts.igem.org/Part:BBa_K1799004).
mutable provenance: This part was synthesized, and is based on E. coli genomic data retrieved via EcoGene: http://www.ecogene.org/gene/EG13807
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1799008_sequence/1

BBa_K1847010
type: ComponentDefinition
persistent identity: BBa K1847010
display id: BBa_K1847010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: lldRO1-plac-lacO-lldRO2
creator: Anna Fomitcheva, Lisa Baumgartner, Charlotte Ramon
created: 2015-08-25T11:00:00Z
modified: 2016-11-15T04:41:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2273_
m user id: 25035
owner id: 26442
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Collection of promoters regulated by LldR and LacI.
mutable provenance: Synthetic synthesis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: plac
sequence annotation: lldRO1
sequence annotation: lldRO2
sequence annotation: lacO
sequence: https://synbiohub.org/public/igem/BBa_K1847010_sequence/1

plac
type: SequenceAnnotation
persistent identity: annotation2438087
display id: annotation2438087
version: 1
top level: BBa_K1847010
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1847010/annotation2438087/range2438087/1

https://synbiohub.org/public/igem/BBa_K1847010/annotation2438087/range2438087/1
type: Range
persistent identity: range2438087
display id: range2438087
version: 1
direction: direction/forward
top level: BBa_K1847010
start: 72
end: 102
orientation: inline

lldRO1
type: SequenceAnnotation
persistent identity: annotation2438086
display id: annotation2438086
version: 1
top level: BBa_K1847010
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K1847010/annotation2438086/range2438086/1

https://synbiohub.org/public/igem/BBa_K1847010/annotation2438086/range2438086/1
type: Range
persistent identity: range2438086
display id: range2438086
version: 1
direction: direction/forward
top level: BBa_K1847010
start: 1
end: 17
orientation: inline

lldRO2
type: SequenceAnnotation
persistent identity: annotation2438089
display id: annotation2438089
version: 1
top level: BBa_K1847010
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K1847010/annotation2438089/range2438089/1

https://synbiohub.org/public/igem/BBa_K1847010/annotation2438089/range2438089/1
type: Range
persistent identity: range2438089
display id: range2438089
version: 1
direction: direction/forward
top level: BBa_K1847010
start: 132
end: 148
orientation: inline

lacO
type: SequenceAnnotation
persistent identity: annotation2438088
display id: annotation2438088
version: 1
top level: BBa_K1847010
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K1847010/annotation2438088/range2438088/1

https://synbiohub.org/public/igem/BBa_K1847010/annotation2438088/range2438088/1
type: Range
persistent identity: range2438088
display id: range2438088
version: 1
direction: direction/forward
top level: BBa_K1847010
start: 110
end: 126
orientation: inline

BBa_K1789000
type: ComponentDefinition
persistent identity: BBa K1789000
display id: BBa_K1789000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: IaaM
creator: Xinyuan Qiu
created: 2015-09-11T11:00:00Z
modified: 2015-09-12T02:27:07Z
discontinued: false
dominant: false
group u list: _2214_
m user id: 21134
owner id: 21134
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This part is the coding sequence of tryptophan-2-mono-oxygenase. This enzyme catalyzes the oxidative carboxylation of L-tryptophan to indole-3-acetamide which acts as an intermediate to the production of indole-3-acetic acid in the IAM pathway.
mutable notes: The reason why er designed this part is that we needed IAA producing pathway as a reporter in our system, and the IAAM and IAAH CDS needed to be fused with the coding sequence of the TAL effector we engineered. So we used the part BBa_K515100, which contains the CDS of IaaM and IaaH, and used PCR to eliminate the RBS sequence from the 5' region of the CDS.
mutable provenance: This part is gained from BBa_K515100 by PCR. The IAA producing pathway originates from Pseudomonas savastanoi.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: IaaM
sequence: https://synbiohub.org/public/igem/BBa_K1789000_sequence/1

IaaM
type: SequenceAnnotation
persistent identity: annotation2452420
display id: annotation2452420
version: 1
top level: BBa_K1789000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1789000/annotation2452420/range2452420/1

https://synbiohub.org/public/igem/BBa_K1789000/annotation2452420/range2452420/1
type: Range
persistent identity: range2452420
display id: range2452420
version: 1
direction: direction/forward
top level: BBa_K1789000
start: 1
end: 1638
orientation: inline

BBa_K1758377
type: ComponentDefinition
persistent identity: BBa K1758377
display id: BBa_K1758377
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Biosensor device for detection of GHB and GBL
creator: Team Bielefeld-CeBiTec 2015
created: 2015-09-09T11:00:00Z
modified: 2015-09-18T07:41:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2180_
m user id: 26563
owner id: 26563
owning group id: 9
status: status/Planning
bookmark: true
mutable description: This part serves as a generator of sfGFP when induced.
mutable notes: ---
mutable provenance: Biobrick assembly
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1758370
component: BBa_K1758376
sequence annotation: BBa_K1758370
sequence annotation: BBa_K1758376
sequence: https://synbiohub.org/public/igem/BBa_K1758377_sequence/1

BBa_K1758370
type: Component
persistent identity: component2448548
display id: component2448548
version: 1
top level: BBa_K1758377
access: public
definition: https://synbiohub.org/public/igem/BBa_K1758370/1

BBa_K1758376
type: Component
persistent identity: component2448545
display id: component2448545
version: 1
top level: BBa_K1758377
access: public
definition: BBa_K1758376

BBa_K1758370
type: SequenceAnnotation
persistent identity: annotation2448548
display id: annotation2448548
version: 1
top level: BBa_K1758377
location: https://synbiohub.org/public/igem/BBa_K1758377/annotation2448548/range2448548/1
component: BBa_K1758370

https://synbiohub.org/public/igem/BBa_K1758377/annotation2448548/range2448548/1
type: Range
persistent identity: range2448548
display id: range2448548
version: 1
direction: direction/reverse
top level: BBa_K1758377
start: 975
end: 1863
orientation: inline

BBa_K1758376
type: SequenceAnnotation
persistent identity: annotation2448545
display id: annotation2448545
version: 1
top level: BBa_K1758377
location: https://synbiohub.org/public/igem/BBa_K1758377/annotation2448545/range2448545/1
component: BBa_K1758376

https://synbiohub.org/public/igem/BBa_K1758377/annotation2448545/range2448545/1
type: Range
persistent identity: range2448545
display id: range2448545
version: 1
direction: direction/reverse
top level: BBa_K1758377
start: 1
end: 966
orientation: inline

BBa_K1592020
type: ComponentDefinition
persistent identity: BBa K1592020
display id: BBa_K1592020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ptrp mutant1
creator: Shuyan Tang
created: 2015-09-08T11:00:00Z
modified: 2015-09-16T08:47:12Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2009_
m user id: 20267
owner id: 20267
owning group id: 9
status: status/Planning
bookmark: false
mutable description: ptrp is a promoter with the trp operator. It will be repressed by trpR (K588000) and LovTAP. Note that the original trp operator contains two SpeI sites。 We remove the illegal sites without affecting its function by site-directed mutagenesis for meeting the requirements of RFC10 and more convenient to be assembled, and finally constructed three ptrp muant, called ptrp mutant1, ptrp mutant2, and ptrp mutant3.
mutable notes: none
mutable provenance: Cloned from BBa_K588001 and mutated by overlap extension PCR.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: A-C
sequence annotation: T-G
sequence: https://synbiohub.org/public/igem/BBa_K1592020_sequence/1

A-C
type: SequenceAnnotation
persistent identity: annotation2456106
display id: annotation2456106
version: 1
top level: BBa_K1592020
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K1592020/annotation2456106/range2456106/1

https://synbiohub.org/public/igem/BBa_K1592020/annotation2456106/range2456106/1
type: Range
persistent identity: range2456106
display id: range2456106
version: 1
direction: direction/forward
top level: BBa_K1592020
start: 54
end: 54
orientation: inline

T-G
type: SequenceAnnotation
persistent identity: annotation2456105
display id: annotation2456105
version: 1
top level: BBa_K1592020
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K1592020/annotation2456105/range2456105/1

https://synbiohub.org/public/igem/BBa_K1592020/annotation2456105/range2456105/1
type: Range
persistent identity: range2456105
display id: range2456105
version: 1
direction: direction/forward
top level: BBa_K1592020
start: 45
end: 45
orientation: inline

BBa_K1829005
type: ComponentDefinition
persistent identity: BBa K1829005
display id: BBa_K1829005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pAga1: mating factor alpha responsive promoter
creator: Nicholas Suen
created: 2015-09-17T11:00:00Z
modified: 2015-09-18T11:13:43Z
discontinued: false
dominant: false
group u list: _2255_
m user id: 27842
owner id: 27842
owning group id: 9
status: status/Planning
bookmark: false
mutable description: pAga1 is a mating factor alpha responsive promoter that is found endogenously in MATa S. Cerevisiae (budding yeast). pAga1 activates the Aga1 gene, which contributes in the S. Cerevisiae mating pathway by supporting cell structure as mating yeast cells project, or "shmoo" towards each other. pAga1 is activated by the presence of mating factor alpha (aka alpha factor) which is endogenously secreted by MATα type cells.
mutable notes: N/A
mutable provenance: pAga1 is found in the genomic sequence of MATa S. Cerevisiae.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K1829005_sequence/1

Dsb-Art175
type: ComponentDefinition
persistent identity: BBa K1659002
display id: BBa_K1659002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Artilysin Art-175 fused at N-terminal with DsbA signal peptide
creator: Wei Chung Kong
created: 2015-08-25T11:00:00Z
modified: 2016-02-01T12:52:09Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2077_
m user id: 4206
owner id: 24524
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Artilysins are an exciting class of enzyme-based antibacterials. Their name is derived from "artificial endolysin" and they exploit the lytic power of bacteriophage-encoded endolysins. Endolysins are peptidoglycan hydrolases produced at the end of the lytic cycle that pass through the cytoplasmic membrane, degrade the peptidoglycan layer and cause the osmotic lysis of the infected bacterial cell, thus liberating the progeny. Purified endolysins have been used to kill Gram-positive pathogens. Gram-negative bacteria, however, have a protective outer membrane containing lipopolysaccharide (LPS) that serves as a barrier against the peptidoglycan hydrolytic activity of endolysins from the outside. To overcome this problem, selected polycationic or amphipathic peptides that locally destabilize the LPS layer have been covalently fused to endolysins. Biers et al. fused the sheep myeloid antimicrobial peptide (SMAP-29) to the N terminus of the endolysin KZ144 to create Artilysin Art-175. Art-175 has been shown to be a highly potent antibacterial that acts in minutes to kill virtually all Pseudomonas aeruginosa strains. [2] BBa_K1659002 is a composite of Art-175 with the N-terminal DsbA signal peptide with a hexahistidine tag. DsbA targets recombinant proteins to the co-translational signal- recognition-particle (SRP)-dependent pathway to mediate secretion out of the bacterial chassis. [2]
mutable notes: -
mutable provenance: Endolysin KZ144 originates from Pseudomonas aeruginosa bacteriophage varphiKZ. SMAP-29 originates from sheep genomic DNA.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: start
sequence annotation: DsbA 2-19 Signal Sequence
sequence annotation: Art-175
sequence annotation: stop
sequence annotation: Hisx6
sequence: https://synbiohub.org/public/igem/BBa_K1659002_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation2445685
display id: annotation2445685
version: 1
top level: Dsb-Art175
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1659002/annotation2445685/range2445685/1

https://synbiohub.org/public/igem/BBa_K1659002/annotation2445685/range2445685/1
type: Range
persistent identity: range2445685
display id: range2445685
version: 1
direction: direction/forward
top level: Dsb-Art175
start: 1
end: 3
orientation: inline

DsbA 2-19 Signal Sequence
type: SequenceAnnotation
persistent identity: annotation2445686
display id: annotation2445686
version: 1
top level: Dsb-Art175
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1659002/annotation2445686/range2445686/1

https://synbiohub.org/public/igem/BBa_K1659002/annotation2445686/range2445686/1
type: Range
persistent identity: range2445686
display id: range2445686
version: 1
direction: direction/forward
top level: Dsb-Art175
start: 4
end: 57
orientation: inline

Art-175
type: SequenceAnnotation
persistent identity: annotation2445687
display id: annotation2445687
version: 1
top level: Dsb-Art175
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1659002/annotation2445687/range2445687/1

https://synbiohub.org/public/igem/BBa_K1659002/annotation2445687/range2445687/1
type: Range
persistent identity: range2445687
display id: range2445687
version: 1
direction: direction/forward
top level: Dsb-Art175
start: 58
end: 924
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2445689
display id: annotation2445689
version: 1
top level: Dsb-Art175
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1659002/annotation2445689/range2445689/1

https://synbiohub.org/public/igem/BBa_K1659002/annotation2445689/range2445689/1
type: Range
persistent identity: range2445689
display id: range2445689
version: 1
direction: direction/forward
top level: Dsb-Art175
start: 943
end: 948
orientation: inline

Hisx6
type: SequenceAnnotation
persistent identity: annotation2445688
display id: annotation2445688
version: 1
top level: Dsb-Art175
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1659002/annotation2445688/range2445688/1

https://synbiohub.org/public/igem/BBa_K1659002/annotation2445688/range2445688/1
type: Range
persistent identity: range2445688
display id: range2445688
version: 1
direction: direction/forward
top level: Dsb-Art175
start: 925
end: 942
orientation: inline

BBa_K1632005
type: ComponentDefinition
persistent identity: BBa K1632005
display id: BBa_K1632005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: fim switch[default OFF](wild-type)
creator: Riku Shinohara
created: 2015-08-29T11:00:00Z
modified: 2015-09-15T12:54:57Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2049_
m user id: 24760
owner id: 22687
owning group id: 9
status: status/Planning
bookmark: false
mutable description: a
mutable notes: a
mutable provenance: a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K1632005_sequence/1

RBS-His-Sc
type: ComponentDefinition
persistent identity: BBa K1602031
display id: BBa_K1602031
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: HisTag-Scaffold_Si4-Tag fusion protein with RBS BBa_B0034
creator: Sebastian Barthel, Niklas Hummel, Jasmin Weber, Michelle Zoeller, Marcus Geissler, Stefan Bohn, Simon Linder
created: 2015-09-15T11:00:00Z
modified: 2015-09-16T07:00:33Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2019_
m user id: 22022
owner id: 22022
owning group id: 9
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0034
component: BBa_K1602030
sequence annotation: BBa_B0034
sequence annotation: BBa_K1602030
sequence: https://synbiohub.org/public/igem/BBa_K1602031_sequence/1

BBa_B0034
type: Component
persistent identity: component2462920
display id: component2462920
version: 1
top level: RBS-His-Sc
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1602030
type: Component
persistent identity: component2462921
display id: component2462921
version: 1
top level: RBS-His-Sc
access: public
definition: https://synbiohub.org/public/igem/BBa_K1602030/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2462920
display id: annotation2462920
version: 1
top level: RBS-His-Sc
location: https://synbiohub.org/public/igem/BBa_K1602031/annotation2462920/range2462920/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1602031/annotation2462920/range2462920/1
type: Range
persistent identity: range2462920
display id: range2462920
version: 1
direction: direction/reverse
top level: RBS-His-Sc
start: 1
end: 12
orientation: inline

BBa_K1602030
type: SequenceAnnotation
persistent identity: annotation2462921
display id: annotation2462921
version: 1
top level: RBS-His-Sc
location: https://synbiohub.org/public/igem/BBa_K1602031/annotation2462921/range2462921/1
component: BBa_K1602030

https://synbiohub.org/public/igem/BBa_K1602031/annotation2462921/range2462921/1
type: Range
persistent identity: range2462921
display id: range2462921
version: 1
direction: direction/reverse
top level: RBS-His-Sc
start: 19
end: 951
orientation: inline

BBa_K1614001
type: ComponentDefinition
persistent identity: BBa K1614001
display id: BBa_K1614001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Hepatitis Delta Virus(HDV) Ribozyme
creator: Stefan Holderbach
created: 2015-09-13T11:00:00Z
modified: 2015-09-14T07:59:27Z
discontinued: false
dominant: false
group u list: _2031_
m user id: 12380
owner id: 12380
owning group id: 9
status: status/Planning
bookmark: false
mutable description: The hepatitis delta virus ribozyme shows a specific and highly efficient cleavage activity at the RNAs 5' end of the sequence. Therefore it is highly suitable to create defined ends for functional RNA expression.
mutable notes: Cleavage occurs upstream of the G on the 5' end.
mutable provenance: Optimized sequence derived from hepatitis delta virus genome.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence: https://synbiohub.org/public/igem/BBa_K1614001_sequence/1

BBa_K1763423
type: ComponentDefinition
persistent identity: BBa K1763423
display id: BBa_K1763423
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: MaSp1-SeqAB2
creator: Vinson Lam
created: 2015-09-14T11:00:00Z
modified: 2016-02-10T11:27:38Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2185_
m user id: 4206
owner id: 27136
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This part contains the core sequence of MaSp2 that has been assembled with a 5'-AGTT-3' overhang on the 5' end of the sequence and 3'-ACAG-5' overhang on the 3' end of the sequence. This part was designed for use with ICA, along with parts BBa_K1763003 and BBa_K1763004 as an efficient method to assemble multiple MaSp2 sequences together. This setup is to show that this particular set of sticky ends is suitable for ICA. Before using in ICA, this part should be digested with BsaI, a type IIs restriction enzyme, which cuts DNA outside of its recognition site. Sequence and Features
mutable notes: The gene sequence for MaSp2 is particularly GC-rich, which may complicate PCR.
mutable provenance: This part is derived from the MaSp2 gene found in the spider N. Clavipes.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: BsaI recognition site (forward)
sequence annotation: BsaI recognition site (reverse)
sequence annotation: MaSp1 SeqF Primer
sequence annotation: A sticky end
sequence annotation: MaSp1 Core Sequence
sequence annotation: B sticky end
sequence annotation: MaSp1 SeqR Primer
sequence: https://synbiohub.org/public/igem/BBa_K1763423_sequence/1

BsaI recognition site (forward)
type: SequenceAnnotation
persistent identity: annotation2463789
display id: annotation2463789
version: 1
top level: BBa_K1763423
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1763423/annotation2463789/range2463789/1

https://synbiohub.org/public/igem/BBa_K1763423/annotation2463789/range2463789/1
type: Range
persistent identity: range2463789
display id: range2463789
version: 1
direction: direction/forward
top level: BBa_K1763423
start: 1
end: 5
orientation: inline

BsaI recognition site (reverse)
type: SequenceAnnotation
persistent identity: annotation2463790
display id: annotation2463790
version: 1
top level: BBa_K1763423
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1763423/annotation2463790/range2463790/1

https://synbiohub.org/public/igem/BBa_K1763423/annotation2463790/range2463790/1
type: Range
persistent identity: range2463790
display id: range2463790
version: 1
direction: direction/reverse
top level: BBa_K1763423
start: 114
end: 119
orientation: inline

MaSp1 SeqF Primer
type: SequenceAnnotation
persistent identity: annotation2473473
display id: annotation2473473
version: 1
top level: BBa_K1763423
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K1763423/annotation2473473/range2473473/1

https://synbiohub.org/public/igem/BBa_K1763423/annotation2473473/range2473473/1
type: Range
persistent identity: range2473473
display id: range2473473
version: 1
direction: direction/forward
top level: BBa_K1763423
start: 41
end: 58
orientation: inline

A sticky end
type: SequenceAnnotation
persistent identity: annotation2463791
display id: annotation2463791
version: 1
top level: BBa_K1763423
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1763423/annotation2463791/range2463791/1

https://synbiohub.org/public/igem/BBa_K1763423/annotation2463791/range2463791/1
type: Range
persistent identity: range2463791
display id: range2463791
version: 1
direction: direction/forward
top level: BBa_K1763423
start: 7
end: 10
orientation: inline

MaSp1 Core Sequence
type: SequenceAnnotation
persistent identity: annotation2473472
display id: annotation2473472
version: 1
top level: BBa_K1763423
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763423/annotation2473472/range2473472/1

https://synbiohub.org/public/igem/BBa_K1763423/annotation2473472/range2473472/1
type: Range
persistent identity: range2473472
display id: range2473472
version: 1
direction: direction/forward
top level: BBa_K1763423
start: 11
end: 108
orientation: inline

B sticky end
type: SequenceAnnotation
persistent identity: annotation2463793
display id: annotation2463793
version: 1
top level: BBa_K1763423
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1763423/annotation2463793/range2463793/1

https://synbiohub.org/public/igem/BBa_K1763423/annotation2463793/range2463793/1
type: Range
persistent identity: range2463793
display id: range2463793
version: 1
direction: direction/forward
top level: BBa_K1763423
start: 109
end: 112
orientation: inline

MaSp1 SeqR Primer
type: SequenceAnnotation
persistent identity: annotation2473474
display id: annotation2473474
version: 1
top level: BBa_K1763423
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K1763423/annotation2473474/range2473474/1

https://synbiohub.org/public/igem/BBa_K1763423/annotation2473474/range2473474/1
type: Range
persistent identity: range2473474
display id: range2473474
version: 1
direction: direction/reverse
top level: BBa_K1763423
start: 41
end: 58
orientation: inline

BBa_K1602009
type: ComponentDefinition
persistent identity: BBa K1602009
display id: BBa_K1602009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: xylB
creator: Leon Kraus, Jonathan Pletzer-Zelgert, Jonas Sindlinger, Carmen Sakas Gandullo, Sebastian Holzner, Elena Tonchevska, Mirjam Haun
created: 2015-09-10T11:00:00Z
modified: 2015-09-25T01:22:39Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2019_
m user id: 12512
owner id: 25989
owning group id: 9
status: status/Planning
bookmark: false
mutable description: BLANK
mutable notes: BLANK
mutable provenance: BLANK
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1602009_sequence/1

BBa_K1806004
type: ComponentDefinition
persistent identity: BBa K1806004
display id: BBa_K1806004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7+ ibPB RNA Thermometer + pelB + MCS + 6xHis
creator: İBRAHİM YASİR ORHAN
created: 2015-09-16T11:00:00Z
modified: 2015-09-20T11:13:33Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2231_
m user id: 11155
owner id: 11153
owning group id: 9
status: status/Planning
bookmark: true
mutable description: The part is to act as a backbone for the T7+ibPB RNA Thermometer+pelB+6xHis system. The MCS(Multiple Cloning Site) as the ligation region for the parts to be integrated into the system. The part that is added to the backbone will be translated in the presence of higher temperatures. The pelB signal peptide is present for the produced protein to be carried out of the cell. The 6xHis is for protein analysis.
mutable notes: none
mutable provenance: This is a composite part formed up of distinct parts from distinct origins.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence: https://synbiohub.org/public/igem/BBa_K1806004_sequence/1

BBa_K1763434
type: ComponentDefinition
persistent identity: BBa K1763434
display id: BBa_K1763434
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: MaSp1-9(1C3)
creator: Vinson Lam
created: 2015-09-14T11:00:00Z
modified: 2015-09-16T11:44:18Z
discontinued: false
dominant: false
group u list: _2185_
m user id: 27136
owner id: 27136
owning group id: 9
status: status/Planning
bookmark: false
mutable description: MaSp1 9 mer in pSB1C3, no regulatory elements present.
mutable notes: no.
mutable provenance: N. clavipes
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: M1-AB
sequence annotation: M1-CA
sequence annotation: M1-BC
sequence annotation: 8-his tag
sequence: https://synbiohub.org/public/igem/BBa_K1763434_sequence/1

M1-AB
type: SequenceAnnotation
persistent identity: annotation2463975
display id: annotation2463975
version: 1
top level: BBa_K1763434
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763434/annotation2463975/range2463975/1

https://synbiohub.org/public/igem/BBa_K1763434/annotation2463975/range2463975/1
type: Range
persistent identity: range2463975
display id: range2463975
version: 1
direction: direction/forward
top level: BBa_K1763434
start: 648
end: 749
orientation: inline

M1-CA
type: SequenceAnnotation
persistent identity: annotation2463974
display id: annotation2463974
version: 1
top level: BBa_K1763434
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763434/annotation2463974/range2463974/1

https://synbiohub.org/public/igem/BBa_K1763434/annotation2463974/range2463974/1
type: Range
persistent identity: range2463974
display id: range2463974
version: 1
direction: direction/forward
top level: BBa_K1763434
start: 240
end: 341
orientation: inline

M1-CA
type: SequenceAnnotation
persistent identity: annotation2463976
display id: annotation2463976
version: 1
top level: BBa_K1763434
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763434/annotation2463976/range2463976/1

https://synbiohub.org/public/igem/BBa_K1763434/annotation2463976/range2463976/1
type: Range
persistent identity: range2463976
display id: range2463976
version: 1
direction: direction/forward
top level: BBa_K1763434
start: 852
end: 953
orientation: inline

M1-BC
type: SequenceAnnotation
persistent identity: annotation2463977
display id: annotation2463977
version: 1
top level: BBa_K1763434
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763434/annotation2463977/range2463977/1

https://synbiohub.org/public/igem/BBa_K1763434/annotation2463977/range2463977/1
type: Range
persistent identity: range2463977
display id: range2463977
version: 1
direction: direction/forward
top level: BBa_K1763434
start: 750
end: 851
orientation: inline

M1-AB
type: SequenceAnnotation
persistent identity: annotation2463982
display id: annotation2463982
version: 1
top level: BBa_K1763434
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763434/annotation2463982/range2463982/1

https://synbiohub.org/public/igem/BBa_K1763434/annotation2463982/range2463982/1
type: Range
persistent identity: range2463982
display id: range2463982
version: 1
direction: direction/forward
top level: BBa_K1763434
start: 36
end: 137
orientation: inline

M1-CA
type: SequenceAnnotation
persistent identity: annotation2463979
display id: annotation2463979
version: 1
top level: BBa_K1763434
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763434/annotation2463979/range2463979/1

https://synbiohub.org/public/igem/BBa_K1763434/annotation2463979/range2463979/1
type: Range
persistent identity: range2463979
display id: range2463979
version: 1
direction: direction/forward
top level: BBa_K1763434
start: 546
end: 647
orientation: inline

8-his tag
type: SequenceAnnotation
persistent identity: annotation2463973
display id: annotation2463973
version: 1
top level: BBa_K1763434
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1763434/annotation2463973/range2463973/1

https://synbiohub.org/public/igem/BBa_K1763434/annotation2463973/range2463973/1
type: Range
persistent identity: range2463973
display id: range2463973
version: 1
direction: direction/forward
top level: BBa_K1763434
start: 4
end: 27
orientation: inline

M1-AB
type: SequenceAnnotation
persistent identity: annotation2463981
display id: annotation2463981
version: 1
top level: BBa_K1763434
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763434/annotation2463981/range2463981/1

https://synbiohub.org/public/igem/BBa_K1763434/annotation2463981/range2463981/1
type: Range
persistent identity: range2463981
display id: range2463981
version: 1
direction: direction/forward
top level: BBa_K1763434
start: 342
end: 443
orientation: inline

M1-BC
type: SequenceAnnotation
persistent identity: annotation2463980
display id: annotation2463980
version: 1
top level: BBa_K1763434
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763434/annotation2463980/range2463980/1

https://synbiohub.org/public/igem/BBa_K1763434/annotation2463980/range2463980/1
type: Range
persistent identity: range2463980
display id: range2463980
version: 1
direction: direction/forward
top level: BBa_K1763434
start: 444
end: 545
orientation: inline

M1-BC
type: SequenceAnnotation
persistent identity: annotation2463978
display id: annotation2463978
version: 1
top level: BBa_K1763434
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763434/annotation2463978/range2463978/1

https://synbiohub.org/public/igem/BBa_K1763434/annotation2463978/range2463978/1
type: Range
persistent identity: range2463978
display id: range2463978
version: 1
direction: direction/forward
top level: BBa_K1763434
start: 138
end: 239
orientation: inline

BBa_K1758120
type: ComponentDefinition
persistent identity: BBa K1758120
display id: BBa_K1758120
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RNase E regulating protein RraA
creator: Team Bielefeld-CeBiTec 2015
created: 2015-08-12T11:00:00Z
modified: 2015-09-18T07:05:34Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2180_
m user id: 26563
owner id: 26563
owning group id: 9
status: status/Planning
bookmark: true
mutable description: The product of the RraA-gene has been reported to interact with RNase E from ''E. coli'' and to alternate its activity. Airen showed that if RraA-protein is added to a cell free protein synthesis reaction, the productivity raises about 30% (Airen, 2011). He postulated that activity of RNase E in the reaction is lowered due to the interaction with RraA-protein, therefore mRNA-levels are stabilized. RNase E is brought to the ''in vitro'' reaction by ''E. coli'' cell extract itself.
mutable notes: '''Amplification with Gibson-primer:''' *Amplification of regulator of ribonuclease activity A (''RraA''): '''Forward primer RraA''' 5'-ATGAAATACGATACTTCCGAGCTTTG-3' '''Reverse primer RraA''' 5'-TCATTCAATATCCAGCGGATCTTC-3' *Amplification of the pSB1C3-Backbone: '''Forward primer pSB1C3 RraA(43 bp)''' 5'-TCAGAAGATCCGCTGGATATTGAATGATACTAGTAGCGGCCGCTGCAG-3' '''Reverse primer pSB1C3 RraA(43 bp)''' 5'-TTCTTCCAGTTGTTCCGGGTTTGCCATCTCTAGAAGCGGCCGCGAATTCC-3' '''Ligation via [http://2015.igem.org/Team:Bielefeld-CeBiTec/Protocols Gibson-Assembly].'''
mutable provenance: RraA was obtained via PCR on ''E. coli'' K12 genomic DNA.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: rraA (menG)
sequence: https://synbiohub.org/public/igem/BBa_K1758120_sequence/1

rraA (menG)
type: SequenceAnnotation
persistent identity: annotation2435621
display id: annotation2435621
version: 1
top level: BBa_K1758120
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1758120/annotation2435621/range2435621/1

https://synbiohub.org/public/igem/BBa_K1758120/annotation2435621/range2435621/1
type: Range
persistent identity: range2435621
display id: range2435621
version: 1
direction: direction/forward
top level: BBa_K1758120
start: 1
end: 486
orientation: inline

BBa_K1632018
type: ComponentDefinition
persistent identity: BBa K1632018
display id: BBa_K1632018
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23100_rbs_lasR_TT_Plux_rbs_fimE(wild-type)
creator: Jun kawamura
created: 2015-08-29T11:00:00Z
modified: 2015-09-15T07:54:26Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2049_
m user id: 24760
owner id: 24760
owning group id: 9
status: status/Planning
bookmark: false
mutable description: aaaaaaaa
mutable notes: a
mutable provenance: aa
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1632011
component: BBa_K553003
component: BBa_B0034
component: BBa_R0062
sequence annotation: BBa_K553003
sequence annotation: BBa_K1632011
sequence annotation: BBa_B0034
sequence annotation: BBa_R0062
sequence: https://synbiohub.org/public/igem/BBa_K1632018_sequence/1

BBa_K1632011
type: Component
persistent identity: component2440284
display id: component2440284
version: 1
top level: BBa_K1632018
access: public
definition: BBa_K1632011

BBa_K553003
type: Component
persistent identity: component2440276
display id: component2440276
version: 1
top level: BBa_K1632018
access: public
definition: https://synbiohub.org/public/igem/BBa_K553003/1

BBa_B0034
type: Component
persistent identity: component2440283
display id: component2440283
version: 1
top level: BBa_K1632018
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0062
type: Component
persistent identity: component2440278
display id: component2440278
version: 1
top level: BBa_K1632018
access: public
definition: https://synbiohub.org/public/igem/BBa_R0062/1

BBa_K553003
type: SequenceAnnotation
persistent identity: annotation2440276
display id: annotation2440276
version: 1
top level: BBa_K1632018
location: https://synbiohub.org/public/igem/BBa_K1632018/annotation2440276/range2440276/1
component: BBa_K553003

https://synbiohub.org/public/igem/BBa_K1632018/annotation2440276/range2440276/1
type: Range
persistent identity: range2440276
display id: range2440276
version: 1
direction: direction/reverse
top level: BBa_K1632018
start: 1
end: 921
orientation: inline

BBa_K1632011
type: SequenceAnnotation
persistent identity: annotation2440284
display id: annotation2440284
version: 1
top level: BBa_K1632018
location: https://synbiohub.org/public/igem/BBa_K1632018/annotation2440284/range2440284/1
component: BBa_K1632011

https://synbiohub.org/public/igem/BBa_K1632018/annotation2440284/range2440284/1
type: Range
persistent identity: range2440284
display id: range2440284
version: 1
direction: direction/reverse
top level: BBa_K1632018
start: 1013
end: 1609
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2440283
display id: annotation2440283
version: 1
top level: BBa_K1632018
location: https://synbiohub.org/public/igem/BBa_K1632018/annotation2440283/range2440283/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1632018/annotation2440283/range2440283/1
type: Range
persistent identity: range2440283
display id: range2440283
version: 1
direction: direction/reverse
top level: BBa_K1632018
start: 993
end: 1004
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation2440278
display id: annotation2440278
version: 1
top level: BBa_K1632018
location: https://synbiohub.org/public/igem/BBa_K1632018/annotation2440278/range2440278/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_K1632018/annotation2440278/range2440278/1
type: Range
persistent identity: range2440278
display id: range2440278
version: 1
direction: direction/forward
top level: BBa_K1632018
start: 930
end: 984
orientation: inline

BBa_K1696009
type: ComponentDefinition
persistent identity: BBa K1696009
display id: BBa_K1696009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lactate sensing system
creator: Yue Liu
created: 2015-09-13T11:00:00Z
modified: 2015-09-14T07:07:15Z
discontinued: false
dominant: false
group u list: _2114_
m user id: 16881
owner id: 16881
owning group id: 9
status: status/Planning
bookmark: false
mutable description: We construct a lactate sensing system for our MFC.
mutable notes: Keep special enzyme site to change component of the system in order to regulate parts' expression level.
mutable provenance: From Lactococcus lactis.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: P170
sequence annotation: RPF
sequence annotation: RcfB
sequence: https://synbiohub.org/public/igem/BBa_K1696009_sequence/1

P170
type: SequenceAnnotation
persistent identity: annotation2454076
display id: annotation2454076
version: 1
top level: BBa_K1696009
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1696009/annotation2454076/range2454076/1

https://synbiohub.org/public/igem/BBa_K1696009/annotation2454076/range2454076/1
type: Range
persistent identity: range2454076
display id: range2454076
version: 1
direction: direction/forward
top level: BBa_K1696009
start: 1
end: 418
orientation: inline

RPF
type: SequenceAnnotation
persistent identity: annotation2454078
display id: annotation2454078
version: 1
top level: BBa_K1696009
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1696009/annotation2454078/range2454078/1

https://synbiohub.org/public/igem/BBa_K1696009/annotation2454078/range2454078/1
type: Range
persistent identity: range2454078
display id: range2454078
version: 1
direction: direction/forward
top level: BBa_K1696009
start: 455
end: 1122
orientation: inline

RcfB
type: SequenceAnnotation
persistent identity: annotation2454094
display id: annotation2454094
version: 1
top level: BBa_K1696009
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1696009/annotation2454094/range2454094/1

https://synbiohub.org/public/igem/BBa_K1696009/annotation2454094/range2454094/1
type: Range
persistent identity: range2454094
display id: range2454094
version: 1
direction: direction/forward
top level: BBa_K1696009
start: 1295
end: 1981
orientation: inline

BBa_K1602008
type: ComponentDefinition
persistent identity: BBa K1602008
display id: BBa_K1602008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0034+GRE3
creator: Alexandra Goretzki, Tanja Habeck, Nina Kuschik-Maczollek, Laurin Monnheimer, Steven Pilger, Bianca Reisinger, Daniel Stumpf
created: 2015-09-09T11:00:00Z
modified: 2015-09-25T01:21:40Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2019_
m user id: 12512
owner id: 25989
owning group id: 9
status: status/Planning
bookmark: false
mutable description: BLANK
mutable notes: BLANK
mutable provenance: BLANK
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_K1602004
component: BBa_B0034
sequence annotation: BBa_K1602004
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K1602008_sequence/1

BBa_K1602004
type: Component
persistent identity: component2448559
display id: component2448559
version: 1
top level: BBa_K1602008
access: public
definition: GRE3

BBa_B0034
type: Component
persistent identity: component2448558
display id: component2448558
version: 1
top level: BBa_K1602008
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1602004
type: SequenceAnnotation
persistent identity: annotation2448559
display id: annotation2448559
version: 1
top level: BBa_K1602008
location: https://synbiohub.org/public/igem/BBa_K1602008/annotation2448559/range2448559/1
component: BBa_K1602004

https://synbiohub.org/public/igem/BBa_K1602008/annotation2448559/range2448559/1
type: Range
persistent identity: range2448559
display id: range2448559
version: 1
direction: direction/reverse
top level: BBa_K1602008
start: 19
end: 1002
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2448558
display id: annotation2448558
version: 1
top level: BBa_K1602008
location: https://synbiohub.org/public/igem/BBa_K1602008/annotation2448558/range2448558/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1602008/annotation2448558/range2448558/1
type: Range
persistent identity: range2448558
display id: range2448558
version: 1
direction: direction/reverse
top level: BBa_K1602008
start: 1
end: 12
orientation: inline

BBa_K1692004
type: ComponentDefinition
persistent identity: BBa K1692004
display id: BBa_K1692004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: codon optimized PAL with T7 promoter and Flag Tag
creator: Daniel Kunin
created: 2015-08-02T11:00:00Z
modified: 2015-08-03T01:17:39Z
discontinued: false
dominant: false
group u list: _2110_
m user id: 26209
owner id: 26209
owning group id: 9
status: status/Planning
bookmark: false
mutable description: codon optimized PAL with T7 promoter and Flag Tag
mutable notes: None
mutable provenance: IDT
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1692004_sequence/1

BBa_K1689002
type: ComponentDefinition
persistent identity: BBa K1689002
display id: BBa_K1689002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Coding sequence of Cluc-STV
creator: ZHANG Yihao
created: 2015-09-15T11:00:00Z
modified: 2015-09-27T01:19:10Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2107_
m user id: 26531
owner id: 26464
owning group id: 9
status: status/Planning
bookmark: false
mutable description: including firefly luciferase C terminal 398 amino acid domain, 2??linker, and streptavidin.
mutable notes: firefly luciferase C terminal 398 amino acid domain and streptavidin are linked by 2??linker
mutable provenance: E.coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: streptavidin
sequence annotation: CFluc398
sequence annotation: random spacer
sequence annotation: stop codon
sequence annotation: start codon
sequence annotation: linker
sequence: https://synbiohub.org/public/igem/BBa_K1689002_sequence/1

streptavidin
type: SequenceAnnotation
persistent identity: annotation2469948
display id: annotation2469948
version: 1
top level: BBa_K1689002
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1689002/annotation2469948/range2469948/1

https://synbiohub.org/public/igem/BBa_K1689002/annotation2469948/range2469948/1
type: Range
persistent identity: range2469948
display id: range2469948
version: 1
direction: direction/forward
top level: BBa_K1689002
start: 526
end: 879
orientation: inline

CFluc398
type: SequenceAnnotation
persistent identity: annotation2469944
display id: annotation2469944
version: 1
top level: BBa_K1689002
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1689002/annotation2469944/range2469944/1

https://synbiohub.org/public/igem/BBa_K1689002/annotation2469944/range2469944/1
type: Range
persistent identity: range2469944
display id: range2469944
version: 1
direction: direction/forward
top level: BBa_K1689002
start: 40
end: 495
orientation: inline

random spacer
type: SequenceAnnotation
persistent identity: annotation2477764
display id: annotation2477764
version: 1
top level: BBa_K1689002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1689002/annotation2477764/range2477764/1

https://synbiohub.org/public/igem/BBa_K1689002/annotation2477764/range2477764/1
type: Range
persistent identity: range2477764
display id: range2477764
version: 1
direction: direction/forward
top level: BBa_K1689002
start: 883
end: 937
orientation: inline

stop codon
type: SequenceAnnotation
persistent identity: annotation2469952
display id: annotation2469952
version: 1
top level: BBa_K1689002
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1689002/annotation2469952/range2469952/1

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type: Range
persistent identity: range2469952
display id: range2469952
version: 1
direction: direction/forward
top level: BBa_K1689002
start: 880
end: 882
orientation: inline

start codon
type: SequenceAnnotation
persistent identity: annotation2469942
display id: annotation2469942
version: 1
top level: BBa_K1689002
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1689002/annotation2469942/range2469942/1

https://synbiohub.org/public/igem/BBa_K1689002/annotation2469942/range2469942/1
type: Range
persistent identity: range2469942
display id: range2469942
version: 1
direction: direction/forward
top level: BBa_K1689002
start: 37
end: 39
orientation: inline

random spacer
type: SequenceAnnotation
persistent identity: annotation2477763
display id: annotation2477763
version: 1
top level: BBa_K1689002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1689002/annotation2477763/range2477763/1

https://synbiohub.org/public/igem/BBa_K1689002/annotation2477763/range2477763/1
type: Range
persistent identity: range2477763
display id: range2477763
version: 1
direction: direction/forward
top level: BBa_K1689002
start: 1
end: 36
orientation: inline

linker
type: SequenceAnnotation
persistent identity: annotation2469946
display id: annotation2469946
version: 1
top level: BBa_K1689002
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1689002/annotation2469946/range2469946/1

https://synbiohub.org/public/igem/BBa_K1689002/annotation2469946/range2469946/1
type: Range
persistent identity: range2469946
display id: range2469946
version: 1
direction: direction/forward
top level: BBa_K1689002
start: 496
end: 525
orientation: inline

BBa_K1632006
type: ComponentDefinition
persistent identity: BBa K1632006
display id: BBa_K1632006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: fim switch[default ON](Tokyo_Tech/R0010)
creator: Riku Shinohara
created: 2015-08-29T11:00:00Z
modified: 2015-09-18T07:51:53Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2049_
m user id: 24760
owner id: 22687
owning group id: 9
status: status/Planning
bookmark: false
mutable description: a
mutable notes: a
mutable provenance: a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K1632006_sequence/1

BBa_K1847008
type: ComponentDefinition
persistent identity: BBa K1847008
display id: BBa_K1847008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: lldRO1-J23117-lldRO2
creator: Anna Fomitcheva Khartchenko
created: 2015-08-25T11:00:00Z
modified: 2015-09-17T08:54:56Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2273_
m user id: 26442
owner id: 26442
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Collection of promoters regulated by LldR.
mutable provenance: Synthetic synthesis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: lldRO2
sequence annotation: lldRO1
sequence annotation: J23117
sequence: https://synbiohub.org/public/igem/BBa_K1847008_sequence/1

lldRO2
type: SequenceAnnotation
persistent identity: annotation2438077
display id: annotation2438077
version: 1
top level: BBa_K1847008
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K1847008/annotation2438077/range2438077/1

https://synbiohub.org/public/igem/BBa_K1847008/annotation2438077/range2438077/1
type: Range
persistent identity: range2438077
display id: range2438077
version: 1
direction: direction/forward
top level: BBa_K1847008
start: 127
end: 143
orientation: inline

lldRO1
type: SequenceAnnotation
persistent identity: annotation2438075
display id: annotation2438075
version: 1
top level: BBa_K1847008
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K1847008/annotation2438075/range2438075/1

https://synbiohub.org/public/igem/BBa_K1847008/annotation2438075/range2438075/1
type: Range
persistent identity: range2438075
display id: range2438075
version: 1
direction: direction/forward
top level: BBa_K1847008
start: 1
end: 17
orientation: inline

J23117
type: SequenceAnnotation
persistent identity: annotation2438076
display id: annotation2438076
version: 1
top level: BBa_K1847008
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1847008/annotation2438076/range2438076/1

https://synbiohub.org/public/igem/BBa_K1847008/annotation2438076/range2438076/1
type: Range
persistent identity: range2438076
display id: range2438076
version: 1
direction: direction/forward
top level: BBa_K1847008
start: 72
end: 106
orientation: inline

DspBx
type: ComponentDefinition
persistent identity: BBa K1659210
display id: BBa_K1659210
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Dispersin B with mutation at nucleotide 582 (T to C)
creator: Wei Chung Kong
created: 2015-08-27T11:00:00Z
modified: 2016-02-12T09:06:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2077_
m user id: 4206
owner id: 24524
owning group id: 9
status: status/Planning
bookmark: false
mutable description: to be updated
mutable notes: to be updated
mutable provenance: to be updated
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: start
sequence annotation: Hisx6
sequence annotation: DspBx
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_K1659210_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation2449117
display id: annotation2449117
version: 1
top level: DspBx
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1659210/annotation2449117/range2449117/1

https://synbiohub.org/public/igem/BBa_K1659210/annotation2449117/range2449117/1
type: Range
persistent identity: range2449117
display id: range2449117
version: 1
direction: direction/forward
top level: DspBx
start: 1
end: 3
orientation: inline

Hisx6
type: SequenceAnnotation
persistent identity: annotation2449119
display id: annotation2449119
version: 1
top level: DspBx
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1659210/annotation2449119/range2449119/1

https://synbiohub.org/public/igem/BBa_K1659210/annotation2449119/range2449119/1
type: Range
persistent identity: range2449119
display id: range2449119
version: 1
direction: direction/forward
top level: DspBx
start: 1087
end: 1104
orientation: inline

DspBx
type: SequenceAnnotation
persistent identity: annotation2449118
display id: annotation2449118
version: 1
top level: DspBx
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1659210/annotation2449118/range2449118/1

https://synbiohub.org/public/igem/BBa_K1659210/annotation2449118/range2449118/1
type: Range
persistent identity: range2449118
display id: range2449118
version: 1
direction: direction/forward
top level: DspBx
start: 4
end: 1086
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2453187
display id: annotation2453187
version: 1
top level: DspBx
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1659210/annotation2453187/range2453187/1

https://synbiohub.org/public/igem/BBa_K1659210/annotation2453187/range2453187/1
type: Range
persistent identity: range2453187
display id: range2453187
version: 1
direction: direction/forward
top level: DspBx
start: 1105
end: 1110
orientation: inline

BBa_K1583100
type: ComponentDefinition
persistent identity: BBa K1583100
display id: BBa_K1583100
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pRha + CsgA
creator: Max van 't Hof
created: 2015-09-02T11:00:00Z
modified: 2015-09-18T11:09:29Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2000_
m user id: 24478
owner id: 24478
owning group id: 9
status: status/Planning
bookmark: false
mutable description: CsgA under control of L-rhamnose-inducible promoter
mutable notes: The nucleotides encoding for the second and third amino acid of the CsgA gene were changed to optimize synthesis success. (silent mutations)
mutable provenance: This part was synthesized. The rhamnose promoter was used by the iGEM 2014 TU Delft teama and originally added by the iGEM12 Paris Bettencourt team. RBS and CsgA originate from E. coli K-12 MG1655
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1583050
component: BBa_K1583000
component: BBa_K1583008
component: BBa_K1583051
component: BBa_G0002
component: BBa_K914003
sequence annotation: BBa_K1583000
sequence annotation: BBa_K1583051
sequence annotation: BBa_G0002
sequence annotation: BBa_K914003
sequence annotation: BBa_K1583050
sequence annotation: BBa_K1583008
sequence: https://synbiohub.org/public/igem/BBa_K1583100_sequence/1

BBa_K1583050
type: Component
persistent identity: component2443486
display id: component2443486
version: 1
top level: BBa_K1583100
access: public
definition: https://synbiohub.org/public/igem/BBa_K1583050/1

BBa_K1583000
type: Component
persistent identity: component2443489
display id: component2443489
version: 1
top level: BBa_K1583100
access: public
definition: https://synbiohub.org/public/igem/BBa_K1583000/1

BBa_K1583008
type: Component
persistent identity: component2443487
display id: component2443487
version: 1
top level: BBa_K1583100
access: public
definition: https://synbiohub.org/public/igem/BBa_K1583008/1

BBa_K1583051
type: Component
persistent identity: component2443490
display id: component2443490
version: 1
top level: BBa_K1583100
access: public
definition: https://synbiohub.org/public/igem/BBa_K1583051/1

BBa_G0002
type: Component
persistent identity: component2443483
display id: component2443483
version: 1
top level: BBa_K1583100
access: public
definition: https://synbiohub.org/public/igem/BBa_G0002/1

BBa_K914003
type: Component
persistent identity: component2443485
display id: component2443485
version: 1
top level: BBa_K1583100
access: public
definition: https://synbiohub.org/public/igem/BBa_K914003/1

BBa_K1583000
type: SequenceAnnotation
persistent identity: annotation2443489
display id: annotation2443489
version: 1
top level: BBa_K1583100
location: https://synbiohub.org/public/igem/BBa_K1583100/annotation2443489/range2443489/1
component: BBa_K1583000

https://synbiohub.org/public/igem/BBa_K1583100/annotation2443489/range2443489/1
type: Range
persistent identity: range2443489
display id: range2443489
version: 1
direction: direction/reverse
top level: BBa_K1583100
start: 169
end: 624
orientation: inline

BBa_K1583051
type: SequenceAnnotation
persistent identity: annotation2443490
display id: annotation2443490
version: 1
top level: BBa_K1583100
location: https://synbiohub.org/public/igem/BBa_K1583100/annotation2443490/range2443490/1
component: BBa_K1583051

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type: Range
persistent identity: range2443490
display id: range2443490
version: 1
direction: direction/reverse
top level: BBa_K1583100
start: 625
end: 638
orientation: inline

BBa_G0002
type: SequenceAnnotation
persistent identity: annotation2443483
display id: annotation2443483
version: 1
top level: BBa_K1583100
location: https://synbiohub.org/public/igem/BBa_K1583100/annotation2443483/range2443483/1
component: BBa_G0002

https://synbiohub.org/public/igem/BBa_K1583100/annotation2443483/range2443483/1
type: Range
persistent identity: range2443483
display id: range2443483
version: 1
direction: direction/reverse
top level: BBa_K1583100
start: 1
end: 8
orientation: inline

BBa_K914003
type: SequenceAnnotation
persistent identity: annotation2443485
display id: annotation2443485
version: 1
top level: BBa_K1583100
location: https://synbiohub.org/public/igem/BBa_K1583100/annotation2443485/range2443485/1
component: BBa_K914003

https://synbiohub.org/public/igem/BBa_K1583100/annotation2443485/range2443485/1
type: Range
persistent identity: range2443485
display id: range2443485
version: 1
direction: direction/reverse
top level: BBa_K1583100
start: 9
end: 130
orientation: inline

BBa_K1583050
type: SequenceAnnotation
persistent identity: annotation2443486
display id: annotation2443486
version: 1
top level: BBa_K1583100
location: https://synbiohub.org/public/igem/BBa_K1583100/annotation2443486/range2443486/1
component: BBa_K1583050

https://synbiohub.org/public/igem/BBa_K1583100/annotation2443486/range2443486/1
type: Range
persistent identity: range2443486
display id: range2443486
version: 1
direction: direction/reverse
top level: BBa_K1583100
start: 131
end: 155
orientation: inline

BBa_K1583008
type: SequenceAnnotation
persistent identity: annotation2443487
display id: annotation2443487
version: 1
top level: BBa_K1583100
location: https://synbiohub.org/public/igem/BBa_K1583100/annotation2443487/range2443487/1
component: BBa_K1583008

https://synbiohub.org/public/igem/BBa_K1583100/annotation2443487/range2443487/1
type: Range
persistent identity: range2443487
display id: range2443487
version: 1
direction: direction/reverse
top level: BBa_K1583100
start: 156
end: 167
orientation: inline

BBa_K1689006
type: ComponentDefinition
persistent identity: BBa K1689006
display id: BBa_K1689006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Coding sequence of FKBP-Cluc394
creator: Xiang Li
created: 2015-09-15T11:00:00Z
modified: 2016-01-19T01:28:16Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2107_
m user id: 4206
owner id: 26464
owning group id: 9
status: status/Planning
bookmark: false
mutable description: including FKBP, 2??linker, and firefly luciferase C terminal 394 amino acid domain.
mutable notes: FKBP and C terminal 394 amino acid domain are linked by 2??linker
mutable provenance: E.coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: FKBP
sequence annotation: start codon
sequence annotation: stop codon
sequence annotation: J23110
sequence annotation: B0034
sequence annotation: C394 terminal domain of firefly luciferase
sequence annotation: GGGGSGGGGS linker
sequence: https://synbiohub.org/public/igem/BBa_K1689006_sequence/1

FKBP
type: SequenceAnnotation
persistent identity: annotation2470150
display id: annotation2470150
version: 1
top level: BBa_K1689006
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1689006/annotation2470150/range2470150/1

https://synbiohub.org/public/igem/BBa_K1689006/annotation2470150/range2470150/1
type: Range
persistent identity: range2470150
display id: range2470150
version: 1
direction: direction/forward
top level: BBa_K1689006
start: 51
end: 371
orientation: inline

start codon
type: SequenceAnnotation
persistent identity: annotation2470149
display id: annotation2470149
version: 1
top level: BBa_K1689006
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1689006/annotation2470149/range2470149/1

https://synbiohub.org/public/igem/BBa_K1689006/annotation2470149/range2470149/1
type: Range
persistent identity: range2470149
display id: range2470149
version: 1
direction: direction/forward
top level: BBa_K1689006
start: 48
end: 50
orientation: inline

stop codon
type: SequenceAnnotation
persistent identity: annotation2470155
display id: annotation2470155
version: 1
top level: BBa_K1689006
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1689006/annotation2470155/range2470155/1

https://synbiohub.org/public/igem/BBa_K1689006/annotation2470155/range2470155/1
type: Range
persistent identity: range2470155
display id: range2470155
version: 1
direction: direction/forward
top level: BBa_K1689006
start: 873
end: 875
orientation: inline

J23110
type: SequenceAnnotation
persistent identity: annotation2470146
display id: annotation2470146
version: 1
top level: BBa_K1689006
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1689006/annotation2470146/range2470146/1

https://synbiohub.org/public/igem/BBa_K1689006/annotation2470146/range2470146/1
type: Range
persistent identity: range2470146
display id: range2470146
version: 1
direction: direction/forward
top level: BBa_K1689006
start: 1
end: 35
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2470147
display id: annotation2470147
version: 1
top level: BBa_K1689006
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1689006/annotation2470147/range2470147/1

https://synbiohub.org/public/igem/BBa_K1689006/annotation2470147/range2470147/1
type: Range
persistent identity: range2470147
display id: range2470147
version: 1
direction: direction/forward
top level: BBa_K1689006
start: 36
end: 47
orientation: inline

C394 terminal domain of firefly luciferase
type: SequenceAnnotation
persistent identity: annotation2470154
display id: annotation2470154
version: 1
top level: BBa_K1689006
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1689006/annotation2470154/range2470154/1

https://synbiohub.org/public/igem/BBa_K1689006/annotation2470154/range2470154/1
type: Range
persistent identity: range2470154
display id: range2470154
version: 1
direction: direction/forward
top level: BBa_K1689006
start: 402
end: 872
orientation: inline

GGGGSGGGGS linker
type: SequenceAnnotation
persistent identity: annotation2470152
display id: annotation2470152
version: 1
top level: BBa_K1689006
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1689006/annotation2470152/range2470152/1

https://synbiohub.org/public/igem/BBa_K1689006/annotation2470152/range2470152/1
type: Range
persistent identity: range2470152
display id: range2470152
version: 1
direction: direction/forward
top level: BBa_K1689006
start: 372
end: 401
orientation: inline

BBa_K1758204
type: ComponentDefinition
persistent identity: BBa K1758204
display id: BBa_K1758204
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: His-tagged blc repressor fused to superfolder GFP under the control of the T7 promoter
creator: Team Bielefeld-CeBiTec 2015
created: 2015-08-28T11:00:00Z
modified: 2015-09-18T07:21:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2180_
m user id: 26913
owner id: 26913
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Uses T7 promotor and a strong rbs (BBa_K525998), the blc repressor linked with superfolding GFP (sfGFP) with a His-Tag. By linking sfGFP we want to detect bound protein via fluorescence.
mutable notes: This is a composite part which enables a high protein expression and an easy purification of blcR fused to superfolding GFP (sfGFP). The green fluorescence of the culture is a sign for protein expression.
mutable provenance: We clone blcR in T7-RBS-sfGFP and added a His-Tag via PCR and Gibson Assembly.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: sfGFP
sequence annotation: RBS
sequence annotation: BBa_K525998
sequence annotation: His-Tag
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: T7 promoter
sequence annotation: blcR
sequence: https://synbiohub.org/public/igem/BBa_K1758204_sequence/1

sfGFP
type: SequenceAnnotation
persistent identity: annotation2440052
display id: annotation2440052
version: 1
top level: BBa_K1758204
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1758204/annotation2440052/range2440052/1

https://synbiohub.org/public/igem/BBa_K1758204/annotation2440052/range2440052/1
type: Range
persistent identity: range2440052
display id: range2440052
version: 1
direction: direction/forward
top level: BBa_K1758204
start: 881
end: 1594
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2440046
display id: annotation2440046
version: 1
top level: BBa_K1758204
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1758204/annotation2440046/range2440046/1

https://synbiohub.org/public/igem/BBa_K1758204/annotation2440046/range2440046/1
type: Range
persistent identity: range2440046
display id: range2440046
version: 1
direction: direction/forward
top level: BBa_K1758204
start: 24
end: 27
orientation: inline

BBa_K525998
type: SequenceAnnotation
persistent identity: annotation2440047
display id: annotation2440047
version: 1
top level: BBa_K1758204
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1758204/annotation2440047/range2440047/1

https://synbiohub.org/public/igem/BBa_K1758204/annotation2440047/range2440047/1
type: Range
persistent identity: range2440047
display id: range2440047
version: 1
direction: direction/forward
top level: BBa_K1758204
start: 1
end: 32
orientation: inline

His-Tag
type: SequenceAnnotation
persistent identity: annotation2440053
display id: annotation2440053
version: 1
top level: BBa_K1758204
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1758204/annotation2440053/range2440053/1

https://synbiohub.org/public/igem/BBa_K1758204/annotation2440053/range2440053/1
type: Range
persistent identity: range2440053
display id: range2440053
version: 1
direction: direction/forward
top level: BBa_K1758204
start: 1595
end: 1618
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2440049
display id: annotation2440049
version: 1
top level: BBa_K1758204
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1758204/annotation2440049/range2440049/1

https://synbiohub.org/public/igem/BBa_K1758204/annotation2440049/range2440049/1
type: Range
persistent identity: range2440049
display id: range2440049
version: 1
direction: direction/forward
top level: BBa_K1758204
start: 1715
end: 1755
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2440050
display id: annotation2440050
version: 1
top level: BBa_K1758204
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1758204/annotation2440050/range2440050/1

https://synbiohub.org/public/igem/BBa_K1758204/annotation2440050/range2440050/1
type: Range
persistent identity: range2440050
display id: range2440050
version: 1
direction: direction/forward
top level: BBa_K1758204
start: 1627
end: 1706
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2440048
display id: annotation2440048
version: 1
top level: BBa_K1758204
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1758204/annotation2440048/range2440048/1

https://synbiohub.org/public/igem/BBa_K1758204/annotation2440048/range2440048/1
type: Range
persistent identity: range2440048
display id: range2440048
version: 1
direction: direction/forward
top level: BBa_K1758204
start: 21
end: 32
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2440045
display id: annotation2440045
version: 1
top level: BBa_K1758204
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1758204/annotation2440045/range2440045/1

https://synbiohub.org/public/igem/BBa_K1758204/annotation2440045/range2440045/1
type: Range
persistent identity: range2440045
display id: range2440045
version: 1
direction: direction/forward
top level: BBa_K1758204
start: 1
end: 23
orientation: inline

blcR
type: SequenceAnnotation
persistent identity: annotation2440051
display id: annotation2440051
version: 1
top level: BBa_K1758204
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1758204/annotation2440051/range2440051/1

https://synbiohub.org/public/igem/BBa_K1758204/annotation2440051/range2440051/1
type: Range
persistent identity: range2440051
display id: range2440051
version: 1
direction: direction/forward
top level: BBa_K1758204
start: 50
end: 874
orientation: inline

BBa_K1799014
type: ComponentDefinition
persistent identity: BBa K1799014
display id: BBa_K1799014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LsrB RBS
creator: Will Shindel
created: 2015-08-26T11:00:00Z
modified: 2015-08-27T03:13:56Z
discontinued: false
dominant: false
group u list: _2224_
m user id: 26954
owner id: 26954
owning group id: 9
status: status/Planning
bookmark: false
mutable description: RBS located upstream of the LsrC open reading frame in E. coli
mutable notes: None
mutable provenance: This part was synthesized, and is based on E. coli genomic data retrieved via EcoGene: http://www.ecogene.org/gene/EG13809
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_K1799014_sequence/1

BBa_K1763015
type: ComponentDefinition
persistent identity: BBa K1763015
display id: BBa_K1763015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 Promoter+Silk+sfGFP
creator: Megan Satyadi
created: 2015-08-21T11:00:00Z
modified: 2016-02-10T11:25:59Z
discontinued: false
dominant: false
group u list: _2185_
m user id: 4206
owner id: 27400
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This part is a fusion of the Apis mellifera (Western Honey Bee) silk protein coding region and the superfolder GFP coding region. A T7 promoter regulatory element has been added upstream, along with a ribosomal binding site. (in progress)
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence: https://synbiohub.org/public/igem/BBa_K1763015_sequence/1

BBa_K1795024
type: ComponentDefinition
persistent identity: BBa K1795024
display id: BBa_K1795024
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: KanR Operon under R0010
creator: Joseph L Maniaci
created: 2015-09-16T11:00:00Z
modified: 2015-09-17T10:39:32Z
discontinued: false
dominant: false
group u list: _2220_
m user id: 27446
owner id: 27446
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Provides Kanamycin Resistance to the cell under the Promoter R0010.
mutable notes: N/a
mutable provenance: iGEM Parts Registry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0034
component: BBa_B0015
component: BBa_R0010
component: BBa_K1795300
sequence annotation: BBa_K1795300
sequence annotation: BBa_R0010
sequence annotation: BBa_B0015
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K1795024_sequence/1

BBa_B0034
type: Component
persistent identity: component2468806
display id: component2468806
version: 1
top level: BBa_K1795024
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0015
type: Component
persistent identity: component2468814
display id: component2468814
version: 1
top level: BBa_K1795024
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_R0010
type: Component
persistent identity: component2468798
display id: component2468798
version: 1
top level: BBa_K1795024
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_K1795300
type: Component
persistent identity: component2468807
display id: component2468807
version: 1
top level: BBa_K1795024
access: public
definition: https://synbiohub.org/public/igem/BBa_K1795300/1

BBa_K1795300
type: SequenceAnnotation
persistent identity: annotation2468807
display id: annotation2468807
version: 1
top level: BBa_K1795024
location: https://synbiohub.org/public/igem/BBa_K1795024/annotation2468807/range2468807/1
component: BBa_K1795300

https://synbiohub.org/public/igem/BBa_K1795024/annotation2468807/range2468807/1
type: Range
persistent identity: range2468807
display id: range2468807
version: 1
direction: direction/reverse
top level: BBa_K1795024
start: 227
end: 1042
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2468798
display id: annotation2468798
version: 1
top level: BBa_K1795024
location: https://synbiohub.org/public/igem/BBa_K1795024/annotation2468798/range2468798/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K1795024/annotation2468798/range2468798/1
type: Range
persistent identity: range2468798
display id: range2468798
version: 1
direction: direction/forward
top level: BBa_K1795024
start: 1
end: 200
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2468814
display id: annotation2468814
version: 1
top level: BBa_K1795024
location: https://synbiohub.org/public/igem/BBa_K1795024/annotation2468814/range2468814/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1795024/annotation2468814/range2468814/1
type: Range
persistent identity: range2468814
display id: range2468814
version: 1
direction: direction/reverse
top level: BBa_K1795024
start: 1051
end: 1179
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2468806
display id: annotation2468806
version: 1
top level: BBa_K1795024
location: https://synbiohub.org/public/igem/BBa_K1795024/annotation2468806/range2468806/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1795024/annotation2468806/range2468806/1
type: Range
persistent identity: range2468806
display id: range2468806
version: 1
direction: direction/reverse
top level: BBa_K1795024
start: 209
end: 220
orientation: inline

BBa_K1799013
type: ComponentDefinition
persistent identity: BBa K1799013
display id: BBa_K1799013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LsrD RBS
creator: Will Shindel
created: 2015-08-26T11:00:00Z
modified: 2015-08-27T03:12:19Z
discontinued: false
dominant: false
group u list: _2224_
m user id: 26954
owner id: 26954
owning group id: 9
status: status/Planning
bookmark: false
mutable description: RBS located upstream of the LsrD open reading frame (inside the LsrC open reading frame) in E. coli
mutable notes: None
mutable provenance: This part was synthesized, and is based on E. coli genomic data retrieved via EcoGene: http://www.ecogene.org/gene/EG13809
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_K1799013_sequence/1

BBa_K1763441
type: ComponentDefinition
persistent identity: BBa K1763441
display id: BBa_K1763441
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: MaSp1/2[1:1]-12(T7)
creator: Vinson Lam
created: 2015-09-14T11:00:00Z
modified: 2015-09-15T09:54:37Z
discontinued: false
dominant: false
group u list: _2185_
m user id: 27136
owner id: 27136
owning group id: 9
status: status/Planning
bookmark: false
mutable description: MaSp1 and MaSp2 12-mer block co-polymer under control of T7 promoter and RBS. Ratio of 1 MaSp1 to 1 MaSp2.
mutable notes: no.
mutable provenance: N. clavipes
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K525998
component: BBa_K1763440
sequence annotation: BBa_K1763440
sequence annotation: BBa_K525998
sequence: https://synbiohub.org/public/igem/BBa_K1763441_sequence/1

BBa_K525998
type: Component
persistent identity: component2456990
display id: component2456990
version: 1
top level: BBa_K1763441
access: public
definition: https://synbiohub.org/public/igem/BBa_K525998/1

BBa_K1763440
type: Component
persistent identity: component2456991
display id: component2456991
version: 1
top level: BBa_K1763441
access: public
definition: BBa_K1763440

BBa_K1763440
type: SequenceAnnotation
persistent identity: annotation2456991
display id: annotation2456991
version: 1
top level: BBa_K1763441
location: https://synbiohub.org/public/igem/BBa_K1763441/annotation2456991/range2456991/1
component: BBa_K1763440

https://synbiohub.org/public/igem/BBa_K1763441/annotation2456991/range2456991/1
type: Range
persistent identity: range2456991
display id: range2456991
version: 1
direction: direction/reverse
top level: BBa_K1763441
start: 39
end: 1313
orientation: inline

BBa_K525998
type: SequenceAnnotation
persistent identity: annotation2456990
display id: annotation2456990
version: 1
top level: BBa_K1763441
location: https://synbiohub.org/public/igem/BBa_K1763441/annotation2456990/range2456990/1
component: BBa_K525998

https://synbiohub.org/public/igem/BBa_K1763441/annotation2456990/range2456990/1
type: Range
persistent identity: range2456990
display id: range2456990
version: 1
direction: direction/reverse
top level: BBa_K1763441
start: 1
end: 32
orientation: inline

BBa_K1592017
type: ComponentDefinition
persistent identity: BBa K1592017
display id: BBa_K1592017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Mcfp3 with XPR2 pre
creator: Shuyan Tang
created: 2015-09-08T11:00:00Z
modified: 2015-09-09T07:26:33Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2009_
m user id: 20267
owner id: 20267
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Mcfp-3 is foot protein secreted from Mytilus californianus. The protein is of significance to the formation of byssus to help mussels permanently or temporarily tether to the surface of solid surface of reef or ship-body. This coherent substance shows the excellent adhesion performance with no substitute under water for sustaining the repeating wash of waves and having no toxicity. XPR2 pre is the pre-region corresponds to the signal sequence: the dipeptides XA and XP are substrates for diamino-peptidase; the dibasic KR cleavage site is substrate for Xpr6p endoproteinase (Matoba and Ogrydziak, 1989). The signal tag, XPR2 fused to Mcfp3 can lead the co-translational translocation of heterologous protein Mcfp-3 to be secreted out of the cell to achieve its function, during the progress of which the peptide will be deleted in the Golgi apparatus to eliminate the interference to the secreting protein.
mutable notes: none
mutable provenance: Synthesized by IDT.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: start
sequence annotation: XPR2 signal peptide
sequence annotation: stop
sequence annotation: MCFP3
sequence: https://synbiohub.org/public/igem/BBa_K1592017_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation2456118
display id: annotation2456118
version: 1
top level: BBa_K1592017
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1592017/annotation2456118/range2456118/1

https://synbiohub.org/public/igem/BBa_K1592017/annotation2456118/range2456118/1
type: Range
persistent identity: range2456118
display id: range2456118
version: 1
direction: direction/forward
top level: BBa_K1592017
start: 46
end: 48
orientation: inline

XPR2 signal peptide
type: SequenceAnnotation
persistent identity: annotation2456116
display id: annotation2456116
version: 1
top level: BBa_K1592017
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1592017/annotation2456116/range2456116/1

https://synbiohub.org/public/igem/BBa_K1592017/annotation2456116/range2456116/1
type: Range
persistent identity: range2456116
display id: range2456116
version: 1
direction: direction/forward
top level: BBa_K1592017
start: 1
end: 45
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2456119
display id: annotation2456119
version: 1
top level: BBa_K1592017
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1592017/annotation2456119/range2456119/1

https://synbiohub.org/public/igem/BBa_K1592017/annotation2456119/range2456119/1
type: Range
persistent identity: range2456119
display id: range2456119
version: 1
direction: direction/forward
top level: BBa_K1592017
start: 277
end: 279
orientation: inline

MCFP3
type: SequenceAnnotation
persistent identity: annotation2456117
display id: annotation2456117
version: 1
top level: BBa_K1592017
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1592017/annotation2456117/range2456117/1

https://synbiohub.org/public/igem/BBa_K1592017/annotation2456117/range2456117/1
type: Range
persistent identity: range2456117
display id: range2456117
version: 1
direction: direction/forward
top level: BBa_K1592017
start: 46
end: 279
orientation: inline

BBa_K1763440
type: ComponentDefinition
persistent identity: BBa K1763440
display id: BBa_K1763440
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: MaSp1/2[1:1]-12(1C3)
creator: Vinson Lam
created: 2015-09-14T11:00:00Z
modified: 2015-09-17T12:02:30Z
discontinued: false
dominant: false
group u list: _2185_
m user id: 27136
owner id: 27136
owning group id: 9
status: status/Planning
bookmark: false
mutable description: MaSp1 and MaSp2 12-mer block co-polymer in pSB1C3. No regulatory elements present. Ratio of 1 MaSp1 to 1 MaSp2.
mutable notes: no.
mutable provenance: N. clavipes
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: M2-AB
sequence annotation: M2-BC
sequence annotation: M1-CA
sequence annotation: M1-BC
sequence annotation: M2-CA
sequence annotation: M1-AB
sequence annotation: 8-his tag
sequence: https://synbiohub.org/public/igem/BBa_K1763440_sequence/1

M2-AB
type: SequenceAnnotation
persistent identity: annotation2464107
display id: annotation2464107
version: 1
top level: BBa_K1763440
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763440/annotation2464107/range2464107/1

https://synbiohub.org/public/igem/BBa_K1763440/annotation2464107/range2464107/1
type: Range
persistent identity: range2464107
display id: range2464107
version: 1
direction: direction/forward
top level: BBa_K1763440
start: 648
end: 749
orientation: inline

M2-BC
type: SequenceAnnotation
persistent identity: annotation2464106
display id: annotation2464106
version: 1
top level: BBa_K1763440
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763440/annotation2464106/range2464106/1

https://synbiohub.org/public/igem/BBa_K1763440/annotation2464106/range2464106/1
type: Range
persistent identity: range2464106
display id: range2464106
version: 1
direction: direction/forward
top level: BBa_K1763440
start: 750
end: 851
orientation: inline

M1-CA
type: SequenceAnnotation
persistent identity: annotation2464110
display id: annotation2464110
version: 1
top level: BBa_K1763440
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763440/annotation2464110/range2464110/1

https://synbiohub.org/public/igem/BBa_K1763440/annotation2464110/range2464110/1
type: Range
persistent identity: range2464110
display id: range2464110
version: 1
direction: direction/forward
top level: BBa_K1763440
start: 546
end: 647
orientation: inline

M2-BC
type: SequenceAnnotation
persistent identity: annotation2464103
display id: annotation2464103
version: 1
top level: BBa_K1763440
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763440/annotation2464103/range2464103/1

https://synbiohub.org/public/igem/BBa_K1763440/annotation2464103/range2464103/1
type: Range
persistent identity: range2464103
display id: range2464103
version: 1
direction: direction/forward
top level: BBa_K1763440
start: 1056
end: 1157
orientation: inline

M1-CA
type: SequenceAnnotation
persistent identity: annotation2464109
display id: annotation2464109
version: 1
top level: BBa_K1763440
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763440/annotation2464109/range2464109/1

https://synbiohub.org/public/igem/BBa_K1763440/annotation2464109/range2464109/1
type: Range
persistent identity: range2464109
display id: range2464109
version: 1
direction: direction/forward
top level: BBa_K1763440
start: 240
end: 341
orientation: inline

M1-BC
type: SequenceAnnotation
persistent identity: annotation2464105
display id: annotation2464105
version: 1
top level: BBa_K1763440
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763440/annotation2464105/range2464105/1

https://synbiohub.org/public/igem/BBa_K1763440/annotation2464105/range2464105/1
type: Range
persistent identity: range2464105
display id: range2464105
version: 1
direction: direction/forward
top level: BBa_K1763440
start: 138
end: 239
orientation: inline

M2-CA
type: SequenceAnnotation
persistent identity: annotation2464102
display id: annotation2464102
version: 1
top level: BBa_K1763440
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763440/annotation2464102/range2464102/1

https://synbiohub.org/public/igem/BBa_K1763440/annotation2464102/range2464102/1
type: Range
persistent identity: range2464102
display id: range2464102
version: 1
direction: direction/forward
top level: BBa_K1763440
start: 1158
end: 1259
orientation: inline

M1-AB
type: SequenceAnnotation
persistent identity: annotation2464108
display id: annotation2464108
version: 1
top level: BBa_K1763440
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763440/annotation2464108/range2464108/1

https://synbiohub.org/public/igem/BBa_K1763440/annotation2464108/range2464108/1
type: Range
persistent identity: range2464108
display id: range2464108
version: 1
direction: direction/forward
top level: BBa_K1763440
start: 342
end: 443
orientation: inline

M2-AB
type: SequenceAnnotation
persistent identity: annotation2464104
display id: annotation2464104
version: 1
top level: BBa_K1763440
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763440/annotation2464104/range2464104/1

https://synbiohub.org/public/igem/BBa_K1763440/annotation2464104/range2464104/1
type: Range
persistent identity: range2464104
display id: range2464104
version: 1
direction: direction/forward
top level: BBa_K1763440
start: 954
end: 1055
orientation: inline

8-his tag
type: SequenceAnnotation
persistent identity: annotation2464099
display id: annotation2464099
version: 1
top level: BBa_K1763440
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1763440/annotation2464099/range2464099/1

https://synbiohub.org/public/igem/BBa_K1763440/annotation2464099/range2464099/1
type: Range
persistent identity: range2464099
display id: range2464099
version: 1
direction: direction/forward
top level: BBa_K1763440
start: 4
end: 27
orientation: inline

M1-BC
type: SequenceAnnotation
persistent identity: annotation2464100
display id: annotation2464100
version: 1
top level: BBa_K1763440
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763440/annotation2464100/range2464100/1

https://synbiohub.org/public/igem/BBa_K1763440/annotation2464100/range2464100/1
type: Range
persistent identity: range2464100
display id: range2464100
version: 1
direction: direction/forward
top level: BBa_K1763440
start: 444
end: 545
orientation: inline

M2-CA
type: SequenceAnnotation
persistent identity: annotation2464101
display id: annotation2464101
version: 1
top level: BBa_K1763440
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763440/annotation2464101/range2464101/1

https://synbiohub.org/public/igem/BBa_K1763440/annotation2464101/range2464101/1
type: Range
persistent identity: range2464101
display id: range2464101
version: 1
direction: direction/forward
top level: BBa_K1763440
start: 852
end: 953
orientation: inline

M1-AB
type: SequenceAnnotation
persistent identity: annotation2464111
display id: annotation2464111
version: 1
top level: BBa_K1763440
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763440/annotation2464111/range2464111/1

https://synbiohub.org/public/igem/BBa_K1763440/annotation2464111/range2464111/1
type: Range
persistent identity: range2464111
display id: range2464111
version: 1
direction: direction/forward
top level: BBa_K1763440
start: 36
end: 137
orientation: inline

BBa_K1799006
type: ComponentDefinition
persistent identity: BBa K1799006
display id: BBa_K1799006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LsrB (wild type)
creator: Miranda Halle
created: 2015-08-06T11:00:00Z
modified: 2015-08-07T01:01:56Z
discontinued: false
dominant: false
group u list: _2224_
m user id: 19613
owner id: 19613
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Gene for the B subunit of the LsrACDB transporter, present in E. coli on the LsrACDBFG operon. The B subunit is the autoinducer 2 (AI-2) binding protein in the transporter, which actively brings AI-2 into the cell.
mutable notes: The portion of the LsrACDBFG operon that codes for the AI-2 transporter consists of four open reading frames, which code for A, C, D, and B, respectively. Three of these ORFs overlap with one another: C overlaps with A, and its RBS is contained within the A subunit ORF, and D overlaps with C (although to a lesser extent), and its RBS is contained within the C subunit ORF. From a modularization standpoint, this creates an inefficiency because the wild type ORFs of the A and C subunits both contain functional RBSs, and in the case of the A subunit ORF, an ATG 4bp downstream. To reduce this inefficiency, we have created versions of each subunit ORF that are optimized for modularization. Further explanation is available in the descriptions of those parts.
mutable provenance: This part was synthesized from E. coli genomic data retrieved via EcoGene: http://www.ecogene.org/gene/EG13809
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1799006_sequence/1

BBa_K1598001
type: ComponentDefinition
persistent identity: BBa K1598001
display id: BBa_K1598001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TPH1 (Tryptophan hydroxylase 1)
creator: Marta Napiorkowska
created: 2015-06-17T11:00:00Z
modified: 2015-08-27T02:25:18Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2015_
m user id: 27180
owner id: 27256
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Converts the amino acid tryptophan into 5-HTP, which is a precursor to serotonin. Soon to be edit
mutable notes: Optimised for E.coli, removed illegal restriction enzyme sites
mutable provenance: Human (Homo sapiens)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1598001_sequence/1

BBa_K1789012
type: ComponentDefinition
persistent identity: BBa K1789012
display id: BBa_K1789012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: P+R
creator: Jiaqi Sun
created: 2015-09-13T11:00:00Z
modified: 2015-09-14T07:22:47Z
discontinued: false
dominant: false
group u list: _2214_
m user id: 26298
owner id: 26298
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This part is a promoter. It has a strong RBS restriction site following it which can guarantee the high efficiency of our project.
mutable notes: We want to construct a promoter which has RBS30 itself for RBS30 is small. If we supply a promoter with RBS, the efficiency of building will be high.
mutable provenance: It is formed by our basic parts.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_R0010
component: BBa_K1789002
sequence annotation: BBa_K1789002
sequence annotation: BBa_R0010
sequence: https://synbiohub.org/public/igem/BBa_K1789012_sequence/1

BBa_R0010
type: Component
persistent identity: component2454122
display id: component2454122
version: 1
top level: BBa_K1789012
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_K1789002
type: Component
persistent identity: component2454130
display id: component2454130
version: 1
top level: BBa_K1789012
access: public
definition: BBa_K1789002

BBa_K1789002
type: SequenceAnnotation
persistent identity: annotation2454130
display id: annotation2454130
version: 1
top level: BBa_K1789012
location: https://synbiohub.org/public/igem/BBa_K1789012/annotation2454130/range2454130/1
component: BBa_K1789002

https://synbiohub.org/public/igem/BBa_K1789012/annotation2454130/range2454130/1
type: Range
persistent identity: range2454130
display id: range2454130
version: 1
direction: direction/reverse
top level: BBa_K1789012
start: 209
end: 223
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2454122
display id: annotation2454122
version: 1
top level: BBa_K1789012
location: https://synbiohub.org/public/igem/BBa_K1789012/annotation2454122/range2454122/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K1789012/annotation2454122/range2454122/1
type: Range
persistent identity: range2454122
display id: range2454122
version: 1
direction: direction/forward
top level: BBa_K1789012
start: 1
end: 200
orientation: inline

BBa_K1758105
type: ComponentDefinition
persistent identity: BBa K1758105
display id: BBa_K1758105
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: mRFP generator under control of T7 promoter
creator: Team Bielefeld-CeBiTec 2015
created: 2015-08-26T11:00:00Z
modified: 2015-09-18T04:41:35Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2180_
m user id: 27935
owner id: 26563
owning group id: 9
status: status/Planning
bookmark: false
mutable description: mRFP is synthesized when T7-polymerase is induced.
mutable notes: ---
mutable provenance: Both subparts were obtained from iGEM distribution plates.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K516030
component: BBa_I719005
sequence annotation: BBa_I719005
sequence annotation: BBa_K516030
sequence: https://synbiohub.org/public/igem/BBa_K1758105_sequence/1

BBa_K516030
type: Component
persistent identity: component2439084
display id: component2439084
version: 1
top level: BBa_K1758105
access: public
definition: https://synbiohub.org/public/igem/BBa_K516030/1

BBa_I719005
type: Component
persistent identity: component2439070
display id: component2439070
version: 1
top level: BBa_K1758105
access: public
definition: https://synbiohub.org/public/igem/BBa_I719005/1

BBa_I719005
type: SequenceAnnotation
persistent identity: annotation2439070
display id: annotation2439070
version: 1
top level: BBa_K1758105
location: https://synbiohub.org/public/igem/BBa_K1758105/annotation2439070/range2439070/1
component: BBa_I719005

https://synbiohub.org/public/igem/BBa_K1758105/annotation2439070/range2439070/1
type: Range
persistent identity: range2439070
display id: range2439070
version: 1
direction: direction/reverse
top level: BBa_K1758105
start: 1
end: 23
orientation: inline

BBa_K516030
type: SequenceAnnotation
persistent identity: annotation2439084
display id: annotation2439084
version: 1
top level: BBa_K1758105
location: https://synbiohub.org/public/igem/BBa_K1758105/annotation2439084/range2439084/1
component: BBa_K516030

https://synbiohub.org/public/igem/BBa_K1758105/annotation2439084/range2439084/1
type: Range
persistent identity: range2439084
display id: range2439084
version: 1
direction: direction/reverse
top level: BBa_K1758105
start: 32
end: 895
orientation: inline

eYFP_SH3 g
type: ComponentDefinition
persistent identity: BBa K1602041
display id: BBa_K1602041
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Inducible generator of eYFP with C-terminal SH3 ligand
creator: Jasmin Weber, Michelle Zoeller, Marcus Geissler, Stefan Bohn, Simon Linder, Sebastian Barthel
created: 2015-09-16T11:00:00Z
modified: 2015-09-17T05:30:40Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2019_
m user id: 22022
owner id: 22022
owning group id: 9
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_I719005
component: BBa_K1602038
sequence annotation: BBa_I719005
sequence annotation: BBa_K1602038
sequence: https://synbiohub.org/public/igem/BBa_K1602041_sequence/1

BBa_I719005
type: Component
persistent identity: component2467302
display id: component2467302
version: 1
top level: eYFP_SH3 g
access: public
definition: https://synbiohub.org/public/igem/BBa_I719005/1

BBa_K1602038
type: Component
persistent identity: component2467303
display id: component2467303
version: 1
top level: eYFP_SH3 g
access: public
definition: eYFP_SH3li

BBa_I719005
type: SequenceAnnotation
persistent identity: annotation2467302
display id: annotation2467302
version: 1
top level: eYFP_SH3 g
location: https://synbiohub.org/public/igem/BBa_K1602041/annotation2467302/range2467302/1
component: BBa_I719005

https://synbiohub.org/public/igem/BBa_K1602041/annotation2467302/range2467302/1
type: Range
persistent identity: range2467302
display id: range2467302
version: 1
direction: direction/reverse
top level: eYFP_SH3 g
start: 1
end: 23
orientation: inline

BBa_K1602038
type: SequenceAnnotation
persistent identity: annotation2467303
display id: annotation2467303
version: 1
top level: eYFP_SH3 g
location: https://synbiohub.org/public/igem/BBa_K1602041/annotation2467303/range2467303/1
component: BBa_K1602038

https://synbiohub.org/public/igem/BBa_K1602041/annotation2467303/range2467303/1
type: Range
persistent identity: range2467303
display id: range2467303
version: 1
direction: direction/reverse
top level: eYFP_SH3 g
start: 32
end: 817
orientation: inline

BBa_K1799015
type: ComponentDefinition
persistent identity: BBa K1799015
display id: BBa_K1799015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pYeaR
creator: Will Shindel
created: 2015-08-26T11:00:00Z
modified: 2015-08-27T03:20:08Z
discontinued: false
dominant: false
group u list: _2224_
m user id: 26954
owner id: 26954
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Nitrate sensitive promoter
mutable notes: None
mutable provenance: This part was synthesized from E. coli genomic data retrieved via EcoGene.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K1799015_sequence/1

BBa_K1632000
type: ComponentDefinition
persistent identity: BBa K1632000
display id: BBa_K1632000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: fim switch[deault ON](Tokyo_Tech/J23119)
creator: Riku Shinohara
created: 2015-08-12T11:00:00Z
modified: 2015-09-15T12:53:44Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _2049_
m user id: 24760
owner id: 22687
owning group id: 9
status: status/Planning
bookmark: false
mutable description: a
mutable notes: a
mutable provenance: a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Sall
sequence annotation: J23119 promoter
sequence annotation: Ascl
sequence annotation: BamHl
sequence annotation: Bglll
sequence annotation: Mlul
sequence: https://synbiohub.org/public/igem/BBa_K1632000_sequence/1

Sall
type: SequenceAnnotation
persistent identity: annotation2435247
display id: annotation2435247
version: 1
top level: BBa_K1632000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1632000/annotation2435247/range2435247/1

https://synbiohub.org/public/igem/BBa_K1632000/annotation2435247/range2435247/1
type: Range
persistent identity: range2435247
display id: range2435247
version: 1
direction: direction/forward
top level: BBa_K1632000
start: 319
end: 324
orientation: inline

J23119 promoter
type: SequenceAnnotation
persistent identity: annotation2435250
display id: annotation2435250
version: 1
top level: BBa_K1632000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1632000/annotation2435250/range2435250/1

https://synbiohub.org/public/igem/BBa_K1632000/annotation2435250/range2435250/1
type: Range
persistent identity: range2435250
display id: range2435250
version: 1
direction: direction/forward
top level: BBa_K1632000
start: 339
end: 373
orientation: inline

Ascl
type: SequenceAnnotation
persistent identity: annotation2435252
display id: annotation2435252
version: 1
top level: BBa_K1632000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1632000/annotation2435252/range2435252/1

https://synbiohub.org/public/igem/BBa_K1632000/annotation2435252/range2435252/1
type: Range
persistent identity: range2435252
display id: range2435252
version: 1
direction: direction/forward
top level: BBa_K1632000
start: 380
end: 387
orientation: inline

BamHl
type: SequenceAnnotation
persistent identity: annotation2435249
display id: annotation2435249
version: 1
top level: BBa_K1632000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1632000/annotation2435249/range2435249/1

https://synbiohub.org/public/igem/BBa_K1632000/annotation2435249/range2435249/1
type: Range
persistent identity: range2435249
display id: range2435249
version: 1
direction: direction/forward
top level: BBa_K1632000
start: 333
end: 338
orientation: inline

Ascl
type: SequenceAnnotation
persistent identity: annotation2435248
display id: annotation2435248
version: 1
top level: BBa_K1632000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1632000/annotation2435248/range2435248/1

https://synbiohub.org/public/igem/BBa_K1632000/annotation2435248/range2435248/1
type: Range
persistent identity: range2435248
display id: range2435248
version: 1
direction: direction/forward
top level: BBa_K1632000
start: 325
end: 332
orientation: inline

Bglll
type: SequenceAnnotation
persistent identity: annotation2435251
display id: annotation2435251
version: 1
top level: BBa_K1632000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1632000/annotation2435251/range2435251/1

https://synbiohub.org/public/igem/BBa_K1632000/annotation2435251/range2435251/1
type: Range
persistent identity: range2435251
display id: range2435251
version: 1
direction: direction/forward
top level: BBa_K1632000
start: 374
end: 379
orientation: inline

Mlul
type: SequenceAnnotation
persistent identity: annotation2435253
display id: annotation2435253
version: 1
top level: BBa_K1632000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1632000/annotation2435253/range2435253/1

https://synbiohub.org/public/igem/BBa_K1632000/annotation2435253/range2435253/1
type: Range
persistent identity: range2435253
display id: range2435253
version: 1
direction: direction/forward
top level: BBa_K1632000
start: 388
end: 392
orientation: inline

RBS-GFP_Si
type: ComponentDefinition
persistent identity: BBa K1602025
display id: BBa_K1602025
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: HisTag-GFP_Si4-Tag fusion protein with RBS BBa_B0034
creator: Jasmin Weber, Michelle Zoeller, Marcus Geissler, Stefan Bohn, Simon Linder, Sebastian Barthel
created: 2015-09-15T11:00:00Z
modified: 2015-09-16T05:56:03Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2019_
m user id: 22022
owner id: 22022
owning group id: 9
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0034
component: BBa_K1602024
sequence annotation: BBa_B0034
sequence annotation: BBa_K1602024
sequence: https://synbiohub.org/public/igem/BBa_K1602025_sequence/1

BBa_B0034
type: Component
persistent identity: component2462563
display id: component2462563
version: 1
top level: RBS-GFP_Si
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1602024
type: Component
persistent identity: component2462564
display id: component2462564
version: 1
top level: RBS-GFP_Si
access: public
definition: https://synbiohub.org/public/igem/BBa_K1602024/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2462563
display id: annotation2462563
version: 1
top level: RBS-GFP_Si
location: https://synbiohub.org/public/igem/BBa_K1602025/annotation2462563/range2462563/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1602025/annotation2462563/range2462563/1
type: Range
persistent identity: range2462563
display id: range2462563
version: 1
direction: direction/reverse
top level: RBS-GFP_Si
start: 1
end: 12
orientation: inline

BBa_K1602024
type: SequenceAnnotation
persistent identity: annotation2462564
display id: annotation2462564
version: 1
top level: RBS-GFP_Si
location: https://synbiohub.org/public/igem/BBa_K1602025/annotation2462564/range2462564/1
component: BBa_K1602024

https://synbiohub.org/public/igem/BBa_K1602025/annotation2462564/range2462564/1
type: Range
persistent identity: range2462564
display id: range2462564
version: 1
direction: direction/reverse
top level: RBS-GFP_Si
start: 19
end: 825
orientation: inline

BBa_K1763011
type: ComponentDefinition
persistent identity: BBa K1763011
display id: BBa_K1763011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Major Spidroin Protein 1 (MaSp1) with Sticky Ends BC
creator: Vinson Lam
created: 2015-07-26T11:00:00Z
modified: 2015-09-16T10:44:29Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2185_
m user id: 27136
owner id: 27136
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This part contains the core sequence of MaSp1 that has been assembled with a 5'-TGTC-3' overhang on the 5' end of the sequence and 3'-GCAC-5' overhang on the 3' end of the sequence. This part was designed for use with ICA, along with parts BBa_K1763003 and BBa_K1763004 as an efficient method to assemble multiple MaSp sequences together. This setup is to show that this particular set of sticky ends is suitable for ICA. Before using in ICA, this part should be digested with BsaI, a type IIs restriction enzyme, which cuts DNA outside of its recognition site.
mutable notes: The gene sequence for MaSp2 is particularly GC-rich, which may complicate PCR.
mutable provenance: This part is derived from the MaSp2 gene found in the spider N. Clavipes.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: B sticky end
sequence annotation: BsaI recognition site (forward)
sequence annotation: MaSp1 Core
sequence annotation: BsaI site recognition site (reverse)
sequence annotation: C sticky end
sequence: https://synbiohub.org/public/igem/BBa_K1763011_sequence/1

B sticky end
type: SequenceAnnotation
persistent identity: annotation2463774
display id: annotation2463774
version: 1
top level: BBa_K1763011
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1763011/annotation2463774/range2463774/1

https://synbiohub.org/public/igem/BBa_K1763011/annotation2463774/range2463774/1
type: Range
persistent identity: range2463774
display id: range2463774
version: 1
direction: direction/forward
top level: BBa_K1763011
start: 7
end: 10
orientation: inline

BsaI recognition site (forward)
type: SequenceAnnotation
persistent identity: annotation2433554
display id: annotation2433554
version: 1
top level: BBa_K1763011
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1763011/annotation2433554/range2433554/1

https://synbiohub.org/public/igem/BBa_K1763011/annotation2433554/range2433554/1
type: Range
persistent identity: range2433554
display id: range2433554
version: 1
direction: direction/forward
top level: BBa_K1763011
start: 1
end: 5
orientation: inline

MaSp1 Core
type: SequenceAnnotation
persistent identity: annotation2473360
display id: annotation2473360
version: 1
top level: BBa_K1763011
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763011/annotation2473360/range2473360/1

https://synbiohub.org/public/igem/BBa_K1763011/annotation2473360/range2473360/1
type: Range
persistent identity: range2473360
display id: range2473360
version: 1
direction: direction/forward
top level: BBa_K1763011
start: 11
end: 108
orientation: inline

BsaI site recognition site (reverse)
type: SequenceAnnotation
persistent identity: annotation2433555
display id: annotation2433555
version: 1
top level: BBa_K1763011
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1763011/annotation2433555/range2433555/1

https://synbiohub.org/public/igem/BBa_K1763011/annotation2433555/range2433555/1
type: Range
persistent identity: range2433555
display id: range2433555
version: 1
direction: direction/reverse
top level: BBa_K1763011
start: 114
end: 119
orientation: inline

C sticky end
type: SequenceAnnotation
persistent identity: annotation2463775
display id: annotation2463775
version: 1
top level: BBa_K1763011
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1763011/annotation2463775/range2463775/1

https://synbiohub.org/public/igem/BBa_K1763011/annotation2463775/range2463775/1
type: Range
persistent identity: range2463775
display id: range2463775
version: 1
direction: direction/forward
top level: BBa_K1763011
start: 109
end: 112
orientation: inline

eYFP_GBDli
type: ComponentDefinition
persistent identity: BBa K1602037
display id: BBa_K1602037
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: eYFP with C-terminal GBD ligand and RBS BBa_B0034
creator: Jasmin Weber, Michelle Zoeller, Marcus Geissler, Stefan Bohn, Simon Linder, Sebastian Barthel
created: 2015-09-16T11:00:00Z
modified: 2015-09-17T05:05:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2019_
m user id: 22022
owner id: 22022
owning group id: 9
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K1602037_sequence/1

BBa_K1604022
type: ComponentDefinition
persistent identity: BBa K1604022
display id: BBa_K1604022
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: araC-pBAD + β-carotene + J23100 + blh
creator: Veronica Pinamonti
created: 2015-08-19T11:00:00Z
modified: 2015-09-17T05:19:54Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2021_
m user id: 11821
owner id: 24581
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This biobrick is obtained by the assembly of two parts K1604020 and K1604021. The composite part contains the five genes necessary for the retinal synthesis. The first part contains ctrEIBY under araC-pBAD, these four gene are responsive of beta-carotene production starting from FPP, a colurless precurse presents in E.coli. The second part contains blh under costitutive promoter. It encodes for the beta-carotene15-15'dioxygenase that catalizes the cleavege of a single molecule of beta-carotene into two molecules of retinal.
mutable notes: The two parts was joined together by strandard assembly, before it beta-carotene was amplified by Phusion PCR.
mutable provenance: AraC-pBAD promoter comes from E.coli genome, while ctrEIBY are from Pantoea ananatis (formerly Erwinia uredovora) genome. Blh gene and its promoter are synthetized and codon optimezed by GeneScript.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1604020
component: BBa_K1604021
sequence annotation: BBa_K1604020
sequence annotation: BBa_K1604021
sequence: https://synbiohub.org/public/igem/BBa_K1604022_sequence/1

BBa_K1604020
type: Component
persistent identity: component2449228
display id: component2449228
version: 1
top level: BBa_K1604022
access: public
definition: https://synbiohub.org/public/igem/BBa_K1604020/1

BBa_K1604021
type: Component
persistent identity: component2449205
display id: component2449205
version: 1
top level: BBa_K1604022
access: public
definition: BBa_K1604021

BBa_K1604020
type: SequenceAnnotation
persistent identity: annotation2449228
display id: annotation2449228
version: 1
top level: BBa_K1604022
location: https://synbiohub.org/public/igem/BBa_K1604022/annotation2449228/range2449228/1
component: BBa_K1604020

https://synbiohub.org/public/igem/BBa_K1604022/annotation2449228/range2449228/1
type: Range
persistent identity: range2449228
display id: range2449228
version: 1
direction: direction/reverse
top level: BBa_K1604022
start: 898
end: 6663
orientation: inline

BBa_K1604021
type: SequenceAnnotation
persistent identity: annotation2449205
display id: annotation2449205
version: 1
top level: BBa_K1604022
location: https://synbiohub.org/public/igem/BBa_K1604022/annotation2449205/range2449205/1
component: BBa_K1604021

https://synbiohub.org/public/igem/BBa_K1604022/annotation2449205/range2449205/1
type: Range
persistent identity: range2449205
display id: range2449205
version: 1
direction: direction/reverse
top level: BBa_K1604022
start: 1
end: 889
orientation: inline

BBa_K1758343
type: ComponentDefinition
persistent identity: BBa K1758343
display id: BBa_K1758343
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: MerR activator under constitutive promoter and induceble merT promoter with 5 UTR -sfGFP
creator: Team Bielefeld-CeBiTec 2015
created: 2015-09-07T11:00:00Z
modified: 2016-01-06T03:41:10Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2180_
m user id: 4206
owner id: 26630
owning group id: 9
status: status/Planning
bookmark: false
mutable description: ...
mutable notes: ...
mutable provenance: ...
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1758340
component: BBa_K1758342
sequence annotation: BBa_K1758340
sequence annotation: BBa_K1758342
sequence: https://synbiohub.org/public/igem/BBa_K1758343_sequence/1

BBa_K1758340
type: Component
persistent identity: component2447498
display id: component2447498
version: 1
top level: BBa_K1758343
access: public
definition: https://synbiohub.org/public/igem/BBa_K1758340/1

BBa_K1758342
type: Component
persistent identity: component2447504
display id: component2447504
version: 1
top level: BBa_K1758343
access: public
definition: BBa_K1758342

BBa_K1758340
type: SequenceAnnotation
persistent identity: annotation2447498
display id: annotation2447498
version: 1
top level: BBa_K1758343
location: https://synbiohub.org/public/igem/BBa_K1758343/annotation2447498/range2447498/1
component: BBa_K1758340

https://synbiohub.org/public/igem/BBa_K1758343/annotation2447498/range2447498/1
type: Range
persistent identity: range2447498
display id: range2447498
version: 1
direction: direction/reverse
top level: BBa_K1758343
start: 1
end: 496
orientation: inline

BBa_K1758342
type: SequenceAnnotation
persistent identity: annotation2447504
display id: annotation2447504
version: 1
top level: BBa_K1758343
location: https://synbiohub.org/public/igem/BBa_K1758343/annotation2447504/range2447504/1
component: BBa_K1758342

https://synbiohub.org/public/igem/BBa_K1758343/annotation2447504/range2447504/1
type: Range
persistent identity: range2447504
display id: range2447504
version: 1
direction: direction/reverse
top level: BBa_K1758343
start: 505
end: 1470
orientation: inline

BBa_K1666005
type: ComponentDefinition
persistent identity: BBa K1666005
display id: BBa_K1666005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LsrR of LuxS/AI-2 signaling pathway in Salmonalla
creator: Heming Wang
created: 2015-09-05T11:00:00Z
modified: 2015-09-06T08:10:44Z
discontinued: false
dominant: false
group u list: _2084_
m user id: 23948
owner id: 23948
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Quorum sensing is a process of bacterial cell-to-cell communication involving the production and detection of extracellular signaling molecules called autoinducers. And autoinducer-2 (AI-2) has been proposed to serve as a 'universal signal' for interspecies communication. In the LuxS/AI-2 signaling system of Salmonella Typhimurium, AI-2 response involves ATP binding cassette transporter encoded by genes named Lsr (LuxS regulated). And LsrR is the Transcriptional regulator which is inactivated by binding phospho-AI-2, leading to the transcription of the lsr genes. In our project, we set this protein-coding part under a nisA promoter and try to integrate them in the genome of Lactobacillus or Lactococcus for the final purpose of constructing an integrated AI-2 response pathway of Salmonella in the engineered bacteria.
mutable notes: The lsrR gene was isolated from the genome of Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 using PCR. We added restriction enzyme sites when designing primers, and the assembly is done through the use of restriction sites (cutting and ligating).
mutable provenance: We extracted the genomic DNA of Salmonella enterica subsp. enterica serovar Typhimurium str. LT2, and used PCR to isolate the gene LsrR(Gene ID: 1255600).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: lsrR
sequence: https://synbiohub.org/public/igem/BBa_K1666005_sequence/1

lsrR
type: SequenceAnnotation
persistent identity: annotation2445113
display id: annotation2445113
version: 1
top level: BBa_K1666005
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1666005/annotation2445113/range2445113/1

https://synbiohub.org/public/igem/BBa_K1666005/annotation2445113/range2445113/1
type: Range
persistent identity: range2445113
display id: range2445113
version: 1
direction: direction/forward
top level: BBa_K1666005
start: 1
end: 960
orientation: inline

BBa_K1592000
type: ComponentDefinition
persistent identity: BBa K1592000
display id: BBa_K1592000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LIP2 prepro(signal peptide)
creator: Shuyan Tang
created: 2015-08-27T11:00:00Z
modified: 2015-09-18T09:23:12Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2009_
m user id: 20267
owner id: 20267
owning group id: 9
status: status/Planning
bookmark: false
mutable description: The pre-region of lipase 2 from Yarrowia lipolytica corresponds to the signal sequence; the dipeptides XA and XP are substrates for diamino-peptidase; the dibasic KR cleavage site is substrate for Xpr6p endoproteinase.
mutable notes: No.
mutable provenance: It have been contributed from our lab.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: KR cleavage site
sequence annotation: 4 XA or XP
sequence annotation: LIP2 pro
sequence annotation: Start codon
sequence annotation: LIP2 pre
sequence: https://synbiohub.org/public/igem/BBa_K1592000_sequence/1

KR cleavage site
type: SequenceAnnotation
persistent identity: annotation2444448
display id: annotation2444448
version: 1
top level: BBa_K1592000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1592000/annotation2444448/range2444448/1

https://synbiohub.org/public/igem/BBa_K1592000/annotation2444448/range2444448/1
type: Range
persistent identity: range2444448
display id: range2444448
version: 1
direction: direction/forward
top level: BBa_K1592000
start: 94
end: 99
orientation: inline

4 XA or XP
type: SequenceAnnotation
persistent identity: annotation2444446
display id: annotation2444446
version: 1
top level: BBa_K1592000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1592000/annotation2444446/range2444446/1

https://synbiohub.org/public/igem/BBa_K1592000/annotation2444446/range2444446/1
type: Range
persistent identity: range2444446
display id: range2444446
version: 1
direction: direction/forward
top level: BBa_K1592000
start: 40
end: 63
orientation: inline

LIP2 pro
type: SequenceAnnotation
persistent identity: annotation2444447
display id: annotation2444447
version: 1
top level: BBa_K1592000
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K1592000/annotation2444447/range2444447/1

https://synbiohub.org/public/igem/BBa_K1592000/annotation2444447/range2444447/1
type: Range
persistent identity: range2444447
display id: range2444447
version: 1
direction: direction/forward
top level: BBa_K1592000
start: 64
end: 93
orientation: inline

Start codon
type: SequenceAnnotation
persistent identity: annotation2455904
display id: annotation2455904
version: 1
top level: BBa_K1592000
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1592000/annotation2455904/range2455904/1

https://synbiohub.org/public/igem/BBa_K1592000/annotation2455904/range2455904/1
type: Range
persistent identity: range2455904
display id: range2455904
version: 1
direction: direction/forward
top level: BBa_K1592000
start: 1
end: 3
orientation: inline

LIP2 pre
type: SequenceAnnotation
persistent identity: annotation2444445
display id: annotation2444445
version: 1
top level: BBa_K1592000
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K1592000/annotation2444445/range2444445/1

https://synbiohub.org/public/igem/BBa_K1592000/annotation2444445/range2444445/1
type: Range
persistent identity: range2444445
display id: range2444445
version: 1
direction: direction/forward
top level: BBa_K1592000
start: 1
end: 39
orientation: inline

BBa_K1696015
type: ComponentDefinition
persistent identity: BBa K1696015
display id: BBa_K1696015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Mf-ssrA Tag1 reporter
creator: Yue Liu
created: 2015-09-13T11:00:00Z
modified: 2015-09-15T09:21:00Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2114_
m user id: 16881
owner id: 16881
owning group id: 9
status: status/Planning
bookmark: false
mutable description: We incorporated tag1 into a RFP-based test platform for inducible protein degradation in E. coli.
mutable notes: codon optimization
mutable provenance: E.coli and M. florum.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence: https://synbiohub.org/public/igem/BBa_K1696015_sequence/1

BBa_K1640006
type: ComponentDefinition
persistent identity: BBa K1640006
display id: BBa_K1640006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: psbELJ
creator: Meghan Cook
created: 2015-09-09T11:00:00Z
modified: 2015-09-09T11:28:11Z
discontinued: false
dominant: false
group u list: _2057_
m user id: 16353
owner id: 28565
owning group id: 9
status: status/Planning
bookmark: false
mutable description: n/A
mutable notes: N/A
mutable provenance: N.A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: double terminator
sequence annotation: psbL
sequence annotation: rbs
sequence annotation: psbJ
sequence annotation: Plac
sequence annotation: psbE
sequence: https://synbiohub.org/public/igem/BBa_K1640006_sequence/1

double terminator
type: SequenceAnnotation
persistent identity: annotation2448218
display id: annotation2448218
version: 1
top level: BBa_K1640006
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1640006/annotation2448218/range2448218/1

https://synbiohub.org/public/igem/BBa_K1640006/annotation2448218/range2448218/1
type: Range
persistent identity: range2448218
display id: range2448218
version: 1
direction: direction/forward
top level: BBa_K1640006
start: 577
end: 705
orientation: inline

psbL
type: SequenceAnnotation
persistent identity: annotation2448215
display id: annotation2448215
version: 1
top level: BBa_K1640006
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1640006/annotation2448215/range2448215/1

https://synbiohub.org/public/igem/BBa_K1640006/annotation2448215/range2448215/1
type: Range
persistent identity: range2448215
display id: range2448215
version: 1
direction: direction/forward
top level: BBa_K1640006
start: 318
end: 434
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2448205
display id: annotation2448205
version: 1
top level: BBa_K1640006
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1640006/annotation2448205/range2448205/1

https://synbiohub.org/public/igem/BBa_K1640006/annotation2448205/range2448205/1
type: Range
persistent identity: range2448205
display id: range2448205
version: 1
direction: direction/forward
top level: BBa_K1640006
start: 43
end: 55
orientation: inline

psbJ
type: SequenceAnnotation
persistent identity: annotation2448217
display id: annotation2448217
version: 1
top level: BBa_K1640006
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1640006/annotation2448217/range2448217/1

https://synbiohub.org/public/igem/BBa_K1640006/annotation2448217/range2448217/1
type: Range
persistent identity: range2448217
display id: range2448217
version: 1
direction: direction/forward
top level: BBa_K1640006
start: 448
end: 576
orientation: inline

Plac
type: SequenceAnnotation
persistent identity: annotation2475062
display id: annotation2475062
version: 1
top level: BBa_K1640006
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1640006/annotation2475062/range2475062/1

https://synbiohub.org/public/igem/BBa_K1640006/annotation2475062/range2475062/1
type: Range
persistent identity: range2475062
display id: range2475062
version: 1
direction: direction/forward
top level: BBa_K1640006
start: 3
end: 42
orientation: inline

psbE
type: SequenceAnnotation
persistent identity: annotation2448206
display id: annotation2448206
version: 1
top level: BBa_K1640006
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1640006/annotation2448206/range2448206/1

https://synbiohub.org/public/igem/BBa_K1640006/annotation2448206/range2448206/1
type: Range
persistent identity: range2448206
display id: range2448206
version: 1
direction: direction/forward
top level: BBa_K1640006
start: 56
end: 304
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2448216
display id: annotation2448216
version: 1
top level: BBa_K1640006
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1640006/annotation2448216/range2448216/1

https://synbiohub.org/public/igem/BBa_K1640006/annotation2448216/range2448216/1
type: Range
persistent identity: range2448216
display id: range2448216
version: 1
direction: direction/forward
top level: BBa_K1640006
start: 435
end: 447
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2448214
display id: annotation2448214
version: 1
top level: BBa_K1640006
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1640006/annotation2448214/range2448214/1

https://synbiohub.org/public/igem/BBa_K1640006/annotation2448214/range2448214/1
type: Range
persistent identity: range2448214
display id: range2448214
version: 1
direction: direction/forward
top level: BBa_K1640006
start: 305
end: 317
orientation: inline

BBa_K1758375
type: ComponentDefinition
persistent identity: BBa K1758375
display id: BBa_K1758375
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pblc in front of mRFP+terminator
creator: Team Bielefeld-CeBiTec 2015
created: 2015-08-26T11:00:00Z
modified: 2015-09-18T04:37:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2180_
m user id: 27935
owner id: 26563
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Pblc is the promoter of the blcABC operon from ''Agrobacterium tumefaciens''. It contains operator sites that are recognized by the repressor [http://parts.igem.org/Part:BBa_K1758370 BlcR]. Pblc was described by [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4083662/ Pan et al. 2013].
mutable notes: Pblc was described by [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4083662/ Pan et al. 2013].
mutable provenance: Pblc was cloned in front of [http://parts.igem.org/Part:BBa_K516030 BBa_K516030] in pSB1C3 with Gibson primers.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence annotation: Pblc
sequence annotation: mRFP
sequence annotation: BBa_E1010
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K1758375_sequence/1

Pblc
type: SequenceAnnotation
persistent identity: annotation2438935
display id: annotation2438935
version: 1
top level: BBa_K1758375
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1758375/annotation2438935/range2438935/1

https://synbiohub.org/public/igem/BBa_K1758375/annotation2438935/range2438935/1
type: Range
persistent identity: range2438935
display id: range2438935
version: 1
direction: direction/forward
top level: BBa_K1758375
start: 1
end: 93
orientation: inline

mRFP
type: SequenceAnnotation
persistent identity: annotation2438937
display id: annotation2438937
version: 1
top level: BBa_K1758375
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1758375/annotation2438937/range2438937/1

https://synbiohub.org/public/igem/BBa_K1758375/annotation2438937/range2438937/1
type: Range
persistent identity: range2438937
display id: range2438937
version: 1
direction: direction/forward
top level: BBa_K1758375
start: 94
end: 768
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2438936
display id: annotation2438936
version: 1
top level: BBa_K1758375
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1758375/annotation2438936/range2438936/1

https://synbiohub.org/public/igem/BBa_K1758375/annotation2438936/range2438936/1
type: Range
persistent identity: range2438936
display id: range2438936
version: 1
direction: direction/forward
top level: BBa_K1758375
start: 94
end: 799
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2438961
display id: annotation2438961
version: 1
top level: BBa_K1758375
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1758375/annotation2438961/range2438961/1

https://synbiohub.org/public/igem/BBa_K1758375/annotation2438961/range2438961/1
type: Range
persistent identity: range2438961
display id: range2438961
version: 1
direction: direction/forward
top level: BBa_K1758375
start: 808
end: 936
orientation: inline

BBa_K1696013
type: ComponentDefinition
persistent identity: BBa K1696013
display id: BBa_K1696013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Mf-ssrA Tag1
creator: Yue Liu
created: 2015-09-13T11:00:00Z
modified: 2015-09-15T09:16:11Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2114_
m user id: 16881
owner id: 16881
owning group id: 9
status: status/Planning
bookmark: false
mutable description: The M. florum ssrA tag (mf-ssrA) is degraded by its endogenous Lon protease (mf-Lon) but not by E. coli Lon or ClpXP, and mf-Lon does not recognize or degrade ec-ssrA, providing a protease and cognate degradation tag with orthogonal functionality in E. coli.
mutable notes: codon optimization
mutable provenance: M. florum
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence: https://synbiohub.org/public/igem/BBa_K1696013_sequence/1

BBa_K1692006
type: ComponentDefinition
persistent identity: BBa K1692006
display id: BBa_K1692006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: UbiX with T7 Promoter
creator: Daniel Kunin
created: 2015-08-02T11:00:00Z
modified: 2015-08-03T01:00:08Z
discontinued: false
dominant: false
group u list: _2110_
m user id: 26209
owner id: 26209
owning group id: 9
status: status/Planning
bookmark: false
mutable description: UbiX with T7 Promoter
mutable notes: None...
mutable provenance: Escherichia coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K525998
component: BBa_K1692005
sequence annotation: BBa_K525998
sequence annotation: BBa_K1692005
sequence: https://synbiohub.org/public/igem/BBa_K1692006_sequence/1

BBa_K525998
type: Component
persistent identity: component2433981
display id: component2433981
version: 1
top level: BBa_K1692006
access: public
definition: https://synbiohub.org/public/igem/BBa_K525998/1

BBa_K1692005
type: Component
persistent identity: component2433982
display id: component2433982
version: 1
top level: BBa_K1692006
access: public
definition: BBa_K1692005

BBa_K525998
type: SequenceAnnotation
persistent identity: annotation2433981
display id: annotation2433981
version: 1
top level: BBa_K1692006
location: https://synbiohub.org/public/igem/BBa_K1692006/annotation2433981/range2433981/1
component: BBa_K525998

https://synbiohub.org/public/igem/BBa_K1692006/annotation2433981/range2433981/1
type: Range
persistent identity: range2433981
display id: range2433981
version: 1
direction: direction/reverse
top level: BBa_K1692006
start: 1
end: 32
orientation: inline

BBa_K1692005
type: SequenceAnnotation
persistent identity: annotation2433982
display id: annotation2433982
version: 1
top level: BBa_K1692006
location: https://synbiohub.org/public/igem/BBa_K1692006/annotation2433982/range2433982/1
component: BBa_K1692005

https://synbiohub.org/public/igem/BBa_K1692006/annotation2433982/range2433982/1
type: Range
persistent identity: range2433982
display id: range2433982
version: 1
direction: direction/reverse
top level: BBa_K1692006
start: 39
end: 608
orientation: inline

BBa_K1604021
type: ComponentDefinition
persistent identity: BBa K1604021
display id: BBa_K1604021
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23100 + β-carotene 15,15’-dioxygenase (blh gene)
creator: Veronica Pinamonti
created: 2015-08-17T11:00:00Z
modified: 2015-09-18T03:56:40Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2021_
m user id: 24580
owner id: 24581
owning group id: 9
status: status/Planning
bookmark: false
mutable description: blh encodes for β-carotene 15,15'-dioxygenase, the enzyme that cleaves one molecule of β-carotene into two molecules of retinal
mutable notes: The part was subcloned from pUC57 into pSB1C3
mutable provenance: The sequence of uncultered cluster bacteria SAR86 was synthetized and codon optimized by GeneScript
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1604019
component: BBa_J23100
sequence annotation: BBa_K1604019
sequence annotation: BBa_J23100
sequence: https://synbiohub.org/public/igem/BBa_K1604021_sequence/1

BBa_K1604019
type: Component
persistent identity: component2449242
display id: component2449242
version: 1
top level: BBa_K1604021
access: public
definition: https://synbiohub.org/public/igem/BBa_K1604019/1

BBa_J23100
type: Component
persistent identity: component2449238
display id: component2449238
version: 1
top level: BBa_K1604021
access: public
definition: https://synbiohub.org/public/igem/BBa_J23100/1

BBa_K1604019
type: SequenceAnnotation
persistent identity: annotation2449242
display id: annotation2449242
version: 1
top level: BBa_K1604021
location: https://synbiohub.org/public/igem/BBa_K1604021/annotation2449242/range2449242/1
component: BBa_K1604019

https://synbiohub.org/public/igem/BBa_K1604021/annotation2449242/range2449242/1
type: Range
persistent identity: range2449242
display id: range2449242
version: 1
direction: direction/reverse
top level: BBa_K1604021
start: 44
end: 887
orientation: inline

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2449238
display id: annotation2449238
version: 1
top level: BBa_K1604021
location: https://synbiohub.org/public/igem/BBa_K1604021/annotation2449238/range2449238/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K1604021/annotation2449238/range2449238/1
type: Range
persistent identity: range2449238
display id: range2449238
version: 1
direction: direction/reverse
top level: BBa_K1604021
start: 1
end: 35
orientation: inline

BBa_K1789008
type: ComponentDefinition
persistent identity: BBa K1789008
display id: BBa_K1789008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TALE1
creator: Xinyuan Qiu
created: 2015-09-13T11:00:00Z
modified: 2015-09-18T02:27:57Z
discontinued: false
dominant: false
group u list: _2214_
m user id: 21134
owner id: 21134
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This is a engineered TAL effector that can bind with the DNA sequence 5'-GGAGGCACCGGTGG-3'
mutable notes: The part sequence should be able to correctly and efficiently bind with corresponding DNA, so we used bioinformatics tools to help us designing this sequence.
mutable provenance: This part is built by NJU_CHINA using GATEWAY technique. We standardized this part by ourselves.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: TALE1
sequence annotation: DNA Binding domain
sequence: https://synbiohub.org/public/igem/BBa_K1789008_sequence/1

TALE1
type: SequenceAnnotation
persistent identity: annotation2454114
display id: annotation2454114
version: 1
top level: BBa_K1789008
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1789008/annotation2454114/range2454114/1

https://synbiohub.org/public/igem/BBa_K1789008/annotation2454114/range2454114/1
type: Range
persistent identity: range2454114
display id: range2454114
version: 1
direction: direction/forward
top level: BBa_K1789008
start: 1
end: 2087
orientation: inline

DNA Binding domain
type: SequenceAnnotation
persistent identity: annotation2454113
display id: annotation2454113
version: 1
top level: BBa_K1789008
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1789008/annotation2454113/range2454113/1

https://synbiohub.org/public/igem/BBa_K1789008/annotation2454113/range2454113/1
type: Range
persistent identity: range2454113
display id: range2454113
version: 1
direction: direction/forward
top level: BBa_K1789008
start: 508
end: 1872
orientation: inline

BBa_K1763435
type: ComponentDefinition
persistent identity: BBa K1763435
display id: BBa_K1763435
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: MaSp1-9(T7)
creator: Vinson Lam
created: 2015-09-14T11:00:00Z
modified: 2015-09-15T09:24:50Z
discontinued: false
dominant: false
group u list: _2185_
m user id: 27136
owner id: 27136
owning group id: 9
status: status/Planning
bookmark: false
mutable description: MaSp1 9 mer under control of T7 promoter and RBS
mutable notes: no.
mutable provenance: N. clavipes
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K525998
component: BBa_K1763434
sequence annotation: BBa_K1763434
sequence annotation: BBa_K525998
sequence: https://synbiohub.org/public/igem/BBa_K1763435_sequence/1

BBa_K525998
type: Component
persistent identity: component2456884
display id: component2456884
version: 1
top level: BBa_K1763435
access: public
definition: https://synbiohub.org/public/igem/BBa_K525998/1

BBa_K1763434
type: Component
persistent identity: component2456885
display id: component2456885
version: 1
top level: BBa_K1763435
access: public
definition: BBa_K1763434

BBa_K1763434
type: SequenceAnnotation
persistent identity: annotation2456885
display id: annotation2456885
version: 1
top level: BBa_K1763435
location: https://synbiohub.org/public/igem/BBa_K1763435/annotation2456885/range2456885/1
component: BBa_K1763434

https://synbiohub.org/public/igem/BBa_K1763435/annotation2456885/range2456885/1
type: Range
persistent identity: range2456885
display id: range2456885
version: 1
direction: direction/reverse
top level: BBa_K1763435
start: 39
end: 1007
orientation: inline

BBa_K525998
type: SequenceAnnotation
persistent identity: annotation2456884
display id: annotation2456884
version: 1
top level: BBa_K1763435
location: https://synbiohub.org/public/igem/BBa_K1763435/annotation2456884/range2456884/1
component: BBa_K525998

https://synbiohub.org/public/igem/BBa_K1763435/annotation2456884/range2456884/1
type: Range
persistent identity: range2456884
display id: range2456884
version: 1
direction: direction/reverse
top level: BBa_K1763435
start: 1
end: 32
orientation: inline

BBa_K1602049
type: ComponentDefinition
persistent identity: BBa K1602049
display id: BBa_K1602049
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RRkey
creator: Christian Sator,Stefan Zens, Max Zander, Benedikt Spannenkrebs, Sebastian Jaeger
created: 2015-09-15T11:00:00Z
modified: 2015-09-18T06:28:17Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2019_
m user id: 26220
owner id: 26220
owning group id: 9
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K1602049_sequence/1

BBa_K1632001
type: ComponentDefinition
persistent identity: BBa K1632001
display id: BBa_K1632001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: fim switch[default OFF](Tokyo_Tech/J23119)
creator: Riku Shinohara
created: 2015-08-12T11:00:00Z
modified: 2015-09-15T12:53:21Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _2049_
m user id: 24760
owner id: 22687
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: 1
mutable notes: 1
mutable provenance: 1
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BamH1
sequence annotation: Mlu1
sequence annotation: Bgl2
sequence annotation: J23119 promoter
sequence annotation: Sal1
sequence annotation: Asc1
sequence: https://synbiohub.org/public/igem/BBa_K1632001_sequence/1

BamH1
type: SequenceAnnotation
persistent identity: annotation2435625
display id: annotation2435625
version: 1
top level: BBa_K1632001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1632001/annotation2435625/range2435625/1

https://synbiohub.org/public/igem/BBa_K1632001/annotation2435625/range2435625/1
type: Range
persistent identity: range2435625
display id: range2435625
version: 1
direction: direction/reverse
top level: BBa_K1632001
start: 133
end: 138
orientation: inline

Mlu1
type: SequenceAnnotation
persistent identity: annotation2435623
display id: annotation2435623
version: 1
top level: BBa_K1632001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1632001/annotation2435623/range2435623/1

https://synbiohub.org/public/igem/BBa_K1632001/annotation2435623/range2435623/1
type: Range
persistent identity: range2435623
display id: range2435623
version: 1
direction: direction/reverse
top level: BBa_K1632001
start: 78
end: 83
orientation: inline

Bgl2
type: SequenceAnnotation
persistent identity: annotation2435273
display id: annotation2435273
version: 1
top level: BBa_K1632001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1632001/annotation2435273/range2435273/1

https://synbiohub.org/public/igem/BBa_K1632001/annotation2435273/range2435273/1
type: Range
persistent identity: range2435273
display id: range2435273
version: 1
direction: direction/reverse
top level: BBa_K1632001
start: 92
end: 97
orientation: inline

J23119 promoter
type: SequenceAnnotation
persistent identity: annotation2435624
display id: annotation2435624
version: 1
top level: BBa_K1632001
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1632001/annotation2435624/range2435624/1

https://synbiohub.org/public/igem/BBa_K1632001/annotation2435624/range2435624/1
type: Range
persistent identity: range2435624
display id: range2435624
version: 1
direction: direction/reverse
top level: BBa_K1632001
start: 98
end: 132
orientation: inline

Sal1
type: SequenceAnnotation
persistent identity: annotation2435627
display id: annotation2435627
version: 1
top level: BBa_K1632001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1632001/annotation2435627/range2435627/1

https://synbiohub.org/public/igem/BBa_K1632001/annotation2435627/range2435627/1
type: Range
persistent identity: range2435627
display id: range2435627
version: 1
direction: direction/reverse
top level: BBa_K1632001
start: 147
end: 152
orientation: inline

Asc1
type: SequenceAnnotation
persistent identity: annotation2435626
display id: annotation2435626
version: 1
top level: BBa_K1632001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1632001/annotation2435626/range2435626/1

https://synbiohub.org/public/igem/BBa_K1632001/annotation2435626/range2435626/1
type: Range
persistent identity: range2435626
display id: range2435626
version: 1
direction: direction/reverse
top level: BBa_K1632001
start: 139
end: 146
orientation: inline

Asc1
type: SequenceAnnotation
persistent identity: annotation2435272
display id: annotation2435272
version: 1
top level: BBa_K1632001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1632001/annotation2435272/range2435272/1

https://synbiohub.org/public/igem/BBa_K1632001/annotation2435272/range2435272/1
type: Range
persistent identity: range2435272
display id: range2435272
version: 1
direction: direction/reverse
top level: BBa_K1632001
start: 84
end: 91
orientation: inline

BBa_K1610202
type: ComponentDefinition
persistent identity: BBa K1610202
display id: BBa_K1610202
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ACT + Term
creator: Leon Yim
created: 2015-09-17T11:00:00Z
modified: 2015-09-17T11:31:41Z
discontinued: false
dominant: false
group u list: _2027_
m user id: 22834
owner id: 22834
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This part consists of the human antichymotrypsin with a terminator attached behind it.
mutable notes: None.
mutable provenance: This part comes from a human cDNA clone attached with the iGEM terminator B0015.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1610200
component: BBa_B0015
sequence annotation: BBa_K1610200
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K1610202_sequence/1

BBa_K1610200
type: Component
persistent identity: component2469226
display id: component2469226
version: 1
top level: BBa_K1610202
access: public
definition: https://synbiohub.org/public/igem/BBa_K1610200/1

BBa_B0015
type: Component
persistent identity: component2469233
display id: component2469233
version: 1
top level: BBa_K1610202
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_K1610200
type: SequenceAnnotation
persistent identity: annotation2469226
display id: annotation2469226
version: 1
top level: BBa_K1610202
location: https://synbiohub.org/public/igem/BBa_K1610202/annotation2469226/range2469226/1
component: BBa_K1610200

https://synbiohub.org/public/igem/BBa_K1610202/annotation2469226/range2469226/1
type: Range
persistent identity: range2469226
display id: range2469226
version: 1
direction: direction/reverse
top level: BBa_K1610202
start: 1
end: 1272
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2469233
display id: annotation2469233
version: 1
top level: BBa_K1610202
location: https://synbiohub.org/public/igem/BBa_K1610202/annotation2469233/range2469233/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1610202/annotation2469233/range2469233/1
type: Range
persistent identity: range2469233
display id: range2469233
version: 1
direction: direction/reverse
top level: BBa_K1610202
start: 1281
end: 1409
orientation: inline

BBa_K1847012
type: ComponentDefinition
persistent identity: BBa K1847012
display id: BBa_K1847012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: placUV5-lacO-lldRO1-R2oDNA-lldRO2
creator: Anna Fomitcheva Khartchenko
created: 2015-08-25T11:00:00Z
modified: 2015-08-26T06:41:42Z
discontinued: false
dominant: false
group u list: _2273_
m user id: 26442
owner id: 26442
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Collection of promoters regulated by LldR and LacI.
mutable provenance: Synthetic synthesis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: R2oDNA spacer
sequence annotation: lac UV5
sequence annotation: lldRO1
sequence annotation: lldRO2
sequence annotation: lacO
sequence: https://synbiohub.org/public/igem/BBa_K1847012_sequence/1

R2oDNA spacer
type: SequenceAnnotation
persistent identity: annotation2438097
display id: annotation2438097
version: 1
top level: BBa_K1847012
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1847012/annotation2438097/range2438097/1

https://synbiohub.org/public/igem/BBa_K1847012/annotation2438097/range2438097/1
type: Range
persistent identity: range2438097
display id: range2438097
version: 1
direction: direction/forward
top level: BBa_K1847012
start: 86
end: 194
orientation: inline

lac UV5
type: SequenceAnnotation
persistent identity: annotation2438094
display id: annotation2438094
version: 1
top level: BBa_K1847012
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1847012/annotation2438094/range2438094/1

https://synbiohub.org/public/igem/BBa_K1847012/annotation2438094/range2438094/1
type: Range
persistent identity: range2438094
display id: range2438094
version: 1
direction: direction/forward
top level: BBa_K1847012
start: 1
end: 31
orientation: inline

lldRO1
type: SequenceAnnotation
persistent identity: annotation2438096
display id: annotation2438096
version: 1
top level: BBa_K1847012
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K1847012/annotation2438096/range2438096/1

https://synbiohub.org/public/igem/BBa_K1847012/annotation2438096/range2438096/1
type: Range
persistent identity: range2438096
display id: range2438096
version: 1
direction: direction/forward
top level: BBa_K1847012
start: 69
end: 85
orientation: inline

lldRO2
type: SequenceAnnotation
persistent identity: annotation2438098
display id: annotation2438098
version: 1
top level: BBa_K1847012
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K1847012/annotation2438098/range2438098/1

https://synbiohub.org/public/igem/BBa_K1847012/annotation2438098/range2438098/1
type: Range
persistent identity: range2438098
display id: range2438098
version: 1
direction: direction/forward
top level: BBa_K1847012
start: 195
end: 211
orientation: inline

lacO
type: SequenceAnnotation
persistent identity: annotation2438095
display id: annotation2438095
version: 1
top level: BBa_K1847012
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K1847012/annotation2438095/range2438095/1

https://synbiohub.org/public/igem/BBa_K1847012/annotation2438095/range2438095/1
type: Range
persistent identity: range2438095
display id: range2438095
version: 1
direction: direction/forward
top level: BBa_K1847012
start: 39
end: 55
orientation: inline

BBa_K1602010
type: ComponentDefinition
persistent identity: BBa K1602010
display id: BBa_K1602010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: xylC
creator: Leon Kraus, Jonathan Pletzer-Zelgert, Jonas Sindlinger, Carmen Sakas Gandullo, Sebastian Holzner, Elena Tonchevska, Mirjam Haun
created: 2015-09-13T11:00:00Z
modified: 2015-09-25T01:32:15Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2019_
m user id: 12512
owner id: 25878
owning group id: 9
status: status/Planning
bookmark: false
mutable description: BLANK
mutable notes: BLANK
mutable provenance: BLANK
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1602010_sequence/1

BBa_K1602013
type: ComponentDefinition
persistent identity: BBa K1602013
display id: BBa_K1602013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: yqhD
creator: Leon Kraus, Jonathan Pletzer-Zelgert, Jonas Sindlinger, Carmen Sakas Gandullo, Sebastian Holzner, Elena Tonchevska, Mirjam Haun
created: 2015-09-13T11:00:00Z
modified: 2015-09-18T06:39:43Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2019_
m user id: 26220
owner id: 25878
owning group id: 9
status: status/Planning
bookmark: false
mutable description: BLANK
mutable notes: BLANK
mutable provenance: BLANK
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1602013_sequence/1

BBa_K1724000
type: ComponentDefinition
persistent identity: BBa K1724000
display id: BBa_K1724000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pcada
creator: Zhanru Chen
created: 2015-09-04T11:00:00Z
modified: 2016-01-11T03:19:05Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2144_
m user id: 4206
owner id: 21980
owning group id: 9
status: status/Planning
bookmark: true
mutable description: cada promoter
mutable notes: FP:GTTTCTTCGAATTCGCGGCCGCTTCTAGATTGACTCTGTAGTTGCTACA VP:GTTTCTTCCTGCAGCGGCCGCTACTAGT ATTGCACACC CTGTAGCAAC TACAGA
mutable provenance: OVERLAP PCR
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: cadmium activated promoter
sequence: https://synbiohub.org/public/igem/BBa_K1724000_sequence/1

cadmium activated promoter
type: SequenceAnnotation
persistent identity: annotation2443820
display id: annotation2443820
version: 1
top level: BBa_K1724000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1724000/annotation2443820/range2443820/1

https://synbiohub.org/public/igem/BBa_K1724000/annotation2443820/range2443820/1
type: Range
persistent identity: range2443820
display id: range2443820
version: 1
direction: direction/forward
top level: BBa_K1724000
start: 1
end: 31
orientation: inline