SEGA
type: Collection
persistent identity: SEGA collection
display id: SEGA_collection
version: 1
description: In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.
creator: Carolyn Nicole Bayer
created: 2021-09-09T19:49:27.975Z
created: 2021-09-09T19:59:54.232Z
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https://synbiohub.org/public/SEGA/SEGA052/1
type: ComponentDefinition
persistent identity: SEGA052
display id: SEGA052
version: 1
description: natural linear DNA.
owned by: carbay
owned by: morno
date: 08-SEP-2021
division: UNA
keywords: .
locus: SEGA052
molecule: ds-DNA
organism: unspecified
reference: https://synbiohub.org/user/carbay/SEGA/SEGA052_reference0/1
source: natural DNA sequence
type: DnaRegion
type: SO:0000987
role: SO:0000804
sequence annotation: sfGFP
sequence annotation: high TIR
sequence annotation: rhaB promoter
sequence annotation: rrnC
sequence annotation: L3S3P22 Terminator (synthetic)
sequence annotation: L3S2P11 Terminator
sequence annotation: glmS
sequence annotation: pelB signal sequence
sequence annotation: Promoter 3BCD2
sequence annotation: rhaR
sequence annotation: TcR
sequence annotation: rhaS
sequence annotation: pstS
sequence: https://synbiohub.org/public/SEGA/SEGA052_seq/1

sfGFP
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
codon start: 1
label: sfGFP
note: sfGFP
product: superfolder GFP
translation: RKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDGTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEFVTAAGITHGMDELYK
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA052/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA052/annotation7/range/1

https://synbiohub.org/public/SEGA/SEGA052/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA052/1
start: 2722
end: 3432
orientation: inline

high TIR
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
note: TIR
note: high TIR
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA052/1
role: SO:0000139
location: https://synbiohub.org/public/SEGA/SEGA052/annotation5/range/1

https://synbiohub.org/public/SEGA/SEGA052/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA052/1
start: 2638
end: 2664
orientation: inline

rhaB promoter
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
label: rhaB promoter
note: promoter of the E. coli rhaBAD operon, conferring tight induction with L-rhamnose and repression with D-glucose in the presence of RhaR and RhaS (Giacalone et al., 2006)
note: rhaB promoter
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA052/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA052/annotation4/range/1

https://synbiohub.org/public/SEGA/SEGA052/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA052/1
start: 2508
end: 2626
orientation: inline

rrnC
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
label: rrnC
note: rrnC
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA052/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA052/annotation11/range/1

https://synbiohub.org/public/SEGA/SEGA052/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA052/1
start: 4764
end: 4804
orientation: inline

L3S3P22 Terminator (synthetic)
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
label: L3S3P22 Terminator (synthetic)
note: L3S3P22 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA052/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA052/annotation8/range/1

https://synbiohub.org/public/SEGA/SEGA052/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA052/1
start: 3436
end: 3484
orientation: inline

L3S2P11 Terminator
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
label: L3S2P11 Terminator
note: L3S2P11 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA052/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA052/annotation1/range/1

https://synbiohub.org/public/SEGA/SEGA052/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA052/1
start: 538
end: 594
orientation: inline

glmS
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
codon start: 1
label: glmS
note: glmS
translation: VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA052/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA052/annotation0/range/1

https://synbiohub.org/public/SEGA/SEGA052/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA052/1
start: 1
end: 528
orientation: inline

pelB signal sequence
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
codon start: 1
gene: pelB (fragment)
label: pelB signal sequence
note: pelB signal sequence
product: leader peptide for secretion
translation: MKYLLPTAAAGLLLLAAQPAMA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA052/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA052/annotation6/range/1

https://synbiohub.org/public/SEGA/SEGA052/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA052/1
start: 2656
end: 2721
orientation: inline

Promoter 3BCD2
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
label: Promoter 3BCD2
note: Promoter 3BCD2
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA052/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA052/annotation9/range/1

https://synbiohub.org/public/SEGA/SEGA052/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA052/1
start: 3495
end: 3523
orientation: inline

rhaR
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
codon start: 1
gene: E. coli rhaR
label: rhaR
note: needed for rhamnose-dependent activation of the rhaB promoter
note: rhaR
product: transcriptional activator of rhaR and rhaS
translation: MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQGAIPGFNASAGQPHWRLGSMGMAQARQVIGQLEHESSQHVPFANEMAELLFGQLVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRHLNSQKD
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA052/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA052/annotation2/range/1

https://synbiohub.org/public/SEGA/SEGA052/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA052/1
start: 607
end: 1452
orientation: reverseComplement

TcR
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
codon start: 1
gene: tet
label: TcR
note: TcR
note: confers resistance to tetracycline
product: tetracycline efflux protein
translation: MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHYGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVIFGEDRFRWSATMSGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA052/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA052/annotation10/range/1

https://synbiohub.org/public/SEGA/SEGA052/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA052/1
start: 3563
end: 4753
orientation: inline

rhaS
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
codon start: 1
gene: E. coli rhaS
label: rhaS
note: activated by L-rhamnose
note: rhaS
product: positive regulator of the rhaB promoter
translation: MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHGTGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLLPQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKSSLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQRYLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGFLQ
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA052/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA052/annotation3/range/1

https://synbiohub.org/public/SEGA/SEGA052/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA052/1
start: 1526
end: 2362
orientation: reverseComplement

pstS
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
codon start: 1
label: pstS
note: pstS
translation: MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKWADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA052/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA052/annotation12/range/1

https://synbiohub.org/public/SEGA/SEGA052/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA052/1
start: 4999
end: 5246
orientation: inline

https://synbiohub.org/public/SEGA/SEGA083/1
type: ComponentDefinition
persistent identity: SEGA083
display id: SEGA083
version: 1
description: natural linear DNA.
owned by: carbay
owned by: morno
date: 09-SEP-2021
division: UNA
keywords: .
locus: SEGA083
molecule: ds-DNA
organism: unspecified
reference: https://synbiohub.org/user/carbay/SEGA/SEGA083_reference0/1
source: natural DNA sequence
type: DnaRegion
type: SO:0000987
role: SO:0000804
sequence annotation: Ubiquitin
sequence annotation: Promoter 3BCD2
sequence annotation: rrnC
sequence annotation: GFP
sequence annotation: rhaR
sequence annotation: rhaS
sequence annotation: L3S3P22 Terminator (synthetic)
sequence annotation: galK
sequence annotation: EM7 promoter
sequence annotation: rhaB promoter
sequence annotation: Terminator rrnB T1
sequence annotation: L3S2P11 Terminator
sequence annotation: glmS
sequence annotation: pstS
sequence annotation: high TIR
sequence annotation: TcR
sequence: https://synbiohub.org/public/SEGA/SEGA083_seq/1

Ubiquitin
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
codon start: 1
label: Ubiquitin
note: Ubiquitin
translation: MQIFVKTLTGKTITLEVEPGSAHHHHHHHHHHAGSSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQEKSTLELVLELQNETQSATSDA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA083/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA083/annotation9/range/1

https://synbiohub.org/public/SEGA/SEGA083/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA083/1
start: 3948
end: 4247
orientation: inline

Promoter 3BCD2
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
label: Promoter 3BCD2
note: Promoter 3BCD2
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA083/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA083/annotation12/range/1

https://synbiohub.org/public/SEGA/SEGA083/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA083/1
start: 5030
end: 5058
orientation: inline

rrnC
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
label: rrnC
note: rrnC
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA083/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA083/annotation14/range/1

https://synbiohub.org/public/SEGA/SEGA083/annotation14/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA083/1
start: 6299
end: 6339
orientation: inline

GFP
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
codon start: 1
label: GFP
note: GFP
product: green fluorescent protein
translation: SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYK
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA083/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA083/annotation10/range/1

https://synbiohub.org/public/SEGA/SEGA083/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA083/1
start: 4257
end: 4967
orientation: inline

rhaR
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
codon start: 1
gene: E. coli rhaR
label: rhaR
note: needed for rhamnose-dependent activation of the rhaB promoter
note: rhaR
product: transcriptional activator of rhaR and rhaS
translation: MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQGAIPGFNASAGQPHWRLGSMGMAQARQVIGQLEHESSQHVPFANEMAELLFGQLVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRHLNSQKD
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA083/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA083/annotation5/range/1

https://synbiohub.org/public/SEGA/SEGA083/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA083/1
start: 1899
end: 2744
orientation: reverseComplement

rhaS
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
codon start: 1
gene: E. coli rhaS
label: rhaS
note: activated by L-rhamnose
note: rhaS
product: positive regulator of the rhaB promoter
translation: MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHGTGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLLPQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKSSLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQRYLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGFLQ
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA083/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA083/annotation6/range/1

https://synbiohub.org/public/SEGA/SEGA083/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA083/1
start: 2818
end: 3654
orientation: reverseComplement

L3S3P22 Terminator (synthetic)
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
label: L3S3P22 Terminator (synthetic)
note: L3S3P22 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA083/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA083/annotation11/range/1

https://synbiohub.org/public/SEGA/SEGA083/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA083/1
start: 4971
end: 5019
orientation: inline

galK
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
EC number: 2.7.1.6
codon start: 1
db xref: ASAP:ABE-0002568
db xref: EcoGene:EG10363
db xref: GI:16128725
db xref: GeneID:945358
db xref: UniProtKB/Swiss-Prot:P0A6T3
function: enzyme; Degradation of small molecules: Carbon compounds
gene: galK
gene synonym: ECK0746; galA; JW0740
label: galK
locus tag: b0757
note: galK
product: galactokinase
protein id: NP_415278.1
translation: MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVLPCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLDAPIVAHENYQWANYVRGVVKHLQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQEAENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTLVGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTVEAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGGGFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA083/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA083/annotation2/range/1

https://synbiohub.org/public/SEGA/SEGA083/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA083/1
start: 605
end: 1753
orientation: reverseComplement

EM7 promoter
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
label: EM7 promoter
note: EM7 promoter
note: synthetic bacterial promoter
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA083/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA083/annotation3/range/1

https://synbiohub.org/public/SEGA/SEGA083/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA083/1
start: 1783
end: 1830
orientation: reverseComplement

rhaB promoter
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
label: rhaB promoter
note: promoter of the E. coli rhaBAD operon, conferring tight induction with L-rhamnose and repression with D-glucose in the presence of RhaR and RhaS (Giacalone et al., 2006)
note: rhaB promoter
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA083/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA083/annotation7/range/1

https://synbiohub.org/public/SEGA/SEGA083/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA083/1
start: 3800
end: 3918
orientation: inline

Terminator rrnB T1
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
label: Terminator rrnB T1
note: Terminator rrnB T1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA083/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA083/annotation4/range/1

https://synbiohub.org/public/SEGA/SEGA083/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA083/1
start: 1843
end: 1886
orientation: inline

L3S2P11 Terminator
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
label: L3S2P11 Terminator
note: L3S2P11 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA083/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA083/annotation1/range/1

https://synbiohub.org/public/SEGA/SEGA083/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA083/1
start: 538
end: 594
orientation: inline

glmS
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
codon start: 1
label: glmS
note: glmS
translation: VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA083/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA083/annotation0/range/1

https://synbiohub.org/public/SEGA/SEGA083/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA083/1
start: 1
end: 528
orientation: inline

pstS
type: SequenceAnnotation
persistent identity: annotation15
display id: annotation15
version: 1
codon start: 1
label: pstS
note: pstS
translation: MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKWADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA083/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA083/annotation15/range/1

https://synbiohub.org/public/SEGA/SEGA083/annotation15/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA083/1
start: 6534
end: 6781
orientation: inline

high TIR
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
note: TIR
note: high TIR
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA083/1
role: SO:0000139
location: https://synbiohub.org/public/SEGA/SEGA083/annotation8/range/1

https://synbiohub.org/public/SEGA/SEGA083/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA083/1
start: 3930
end: 3956
orientation: inline

TcR
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
codon start: 1
gene: tet
label: TcR
note: TcR
note: confers resistance to tetracycline
product: tetracycline efflux protein
translation: MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHYGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA083/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA083/annotation13/range/1

https://synbiohub.org/public/SEGA/SEGA083/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA083/1
start: 5098
end: 6288
orientation: inline

https://synbiohub.org/public/SEGA/SEGA004/1
type: ComponentDefinition
persistent identity: SEGA004
display id: SEGA004
version: 1
description: natural linear DNA.
owned by: carbay
owned by: morno
date: 09-SEP-2021
division: UNA
keywords: .
locus: SEGA004
molecule: ds-DNA
organism: unspecified
reference: https://synbiohub.org/user/carbay/SEGA/SEGA004_reference0/1
source: natural DNA sequence
type: DnaRegion
type: SO:0000987
role: SO:0000804
sequence annotation: pstS
sequence annotation: glmS
sequence annotation: RBS
sequence annotation: L3S2P11 Terminator
sequence annotation: lacI
sequence annotation: rrnC
sequence annotation: lac operator
sequence annotation: T7 promoter
sequence annotation: lacI promoter
sequence annotation: TcR
sequence: https://synbiohub.org/public/SEGA/SEGA004_seq/1

pstS
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
codon start: 1
label: pstS
note: pstS
translation: MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKWADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA004/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA004/annotation9/range/1

https://synbiohub.org/public/SEGA/SEGA004/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA004/1
start: 3220
end: 3467
orientation: inline

glmS
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
codon start: 1
label: glmS
note: glmS
translation: VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA004/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA004/annotation0/range/1

https://synbiohub.org/public/SEGA/SEGA004/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA004/1
start: 1
end: 528
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
label: RBS
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA004/1
role: SO:0000139
location: https://synbiohub.org/public/SEGA/SEGA004/annotation6/range/1

https://synbiohub.org/public/SEGA/SEGA004/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA004/1
start: 1850
end: 1861
orientation: inline

L3S2P11 Terminator
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
label: L3S2P11 Terminator
note: L3S2P11 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA004/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA004/annotation1/range/1

https://synbiohub.org/public/SEGA/SEGA004/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA004/1
start: 538
end: 594
orientation: inline

lacI
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
codon start: 1
gene: lacI
label: lacI
note: The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG).
note: lacI
product: lac repressor
translation: MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA004/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA004/annotation2/range/1

https://synbiohub.org/public/SEGA/SEGA004/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA004/1
start: 604
end: 1686
orientation: reverseComplement

rrnC
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
label: rrnC
note: rrnC
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA004/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA004/annotation8/range/1

https://synbiohub.org/public/SEGA/SEGA004/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA004/1
start: 2985
end: 3025
orientation: inline

lac operator
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
bound moiety: lac repressor encoded by lacI
feature type: protein_bind
label: lac operator
note: The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG).
note: lac operator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA004/1
role: SO:0000110
location: https://synbiohub.org/public/SEGA/SEGA004/annotation5/range/1

https://synbiohub.org/public/SEGA/SEGA004/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA004/1
start: 1814
end: 1838
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
label: T7 promoter
note: T7 promoter
note: promoter for bacteriophage T7 RNA polymerase
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA004/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA004/annotation4/range/1

https://synbiohub.org/public/SEGA/SEGA004/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA004/1
start: 1795
end: 1813
orientation: inline

lacI promoter
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
gene: lacI
label: lacI promoter
note: lacI promoter
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA004/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA004/annotation3/range/1

https://synbiohub.org/public/SEGA/SEGA004/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA004/1
start: 1687
end: 1764
orientation: reverseComplement

TcR
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
codon start: 1
gene: tet
label: TcR
note: TcR
note: confers resistance to tetracycline
product: tetracycline efflux protein
translation: VLPGLLRDIVHSDSIASHYGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVIFGEDRFRWSATMSGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA004/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA004/annotation7/range/1

https://synbiohub.org/public/SEGA/SEGA004/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA004/1
start: 1862
end: 2974
orientation: inline

https://synbiohub.org/public/SEGA/SEGA077/1
type: ComponentDefinition
persistent identity: SEGA077
display id: SEGA077
version: 1
description: natural linear DNA.
owned by: carbay
owned by: morno
date: 09-SEP-2021
division: UNA
keywords: .
locus: SEGA077
molecule: ds-DNA
organism: unspecified
reference: https://synbiohub.org/user/carbay/SEGA/SEGA077_reference0/1
source: natural DNA sequence
type: DnaRegion
type: SO:0000987
role: SO:0000804
sequence annotation: high TIR
sequence annotation: L3S3P22 Terminator (synthetic)
sequence annotation: glmS
sequence annotation: rrnC
sequence annotation: BCD
sequence annotation: RiboJ
sequence annotation: GFP
sequence annotation: TcR
sequence annotation: L3S2P11 Terminator
sequence annotation: Promoter 3BCD2
sequence annotation: TetR
sequence annotation: pLacI
sequence annotation: tet1
sequence annotation: pstS
sequence annotation: pTet*
sequence: https://synbiohub.org/public/SEGA/SEGA077_seq/1

high TIR
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
note: TIR
note: high TIR
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA077/1
role: SO:0000139
location: https://synbiohub.org/public/SEGA/SEGA077/annotation7/range/1

https://synbiohub.org/public/SEGA/SEGA077/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA077/1
start: 1505
end: 1531
orientation: inline

L3S3P22 Terminator (synthetic)
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
label: L3S3P22 Terminator (synthetic)
note: L3S3P22 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA077/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA077/annotation10/range/1

https://synbiohub.org/public/SEGA/SEGA077/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA077/1
start: 2293
end: 2341
orientation: inline

glmS
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
codon start: 1
label: glmS
note: glmS
translation: VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA077/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA077/annotation0/range/1

https://synbiohub.org/public/SEGA/SEGA077/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA077/1
start: 1
end: 528
orientation: inline

rrnC
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
label: rrnC
note: rrnC
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA077/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA077/annotation13/range/1

https://synbiohub.org/public/SEGA/SEGA077/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA077/1
start: 3621
end: 3661
orientation: inline

BCD
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
codon start: 1
label: BCD
note: BCD
translation: MKAIFVLKHLNHAKEVF
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA077/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA077/annotation8/range/1

https://synbiohub.org/public/SEGA/SEGA077/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA077/1
start: 1523
end: 1578
orientation: inline

RiboJ
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
label: RiboJ
note: RiboJ
note: insulator: self-cleaving ribozyme RiboJ
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA077/1
role: SO:0001411
location: https://synbiohub.org/public/SEGA/SEGA077/annotation6/range/1

https://synbiohub.org/public/SEGA/SEGA077/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA077/1
start: 1419
end: 1493
orientation: inline

GFP
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
codon start: 1
label: GFP
note: GFP
product: green fluorescent protein
translation: SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYK
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA077/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA077/annotation9/range/1

https://synbiohub.org/public/SEGA/SEGA077/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA077/1
start: 1579
end: 2289
orientation: inline

TcR
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
codon start: 1
gene: tet
label: TcR
note: TcR
note: confers resistance to tetracycline
product: tetracycline efflux protein
translation: MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHYGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVIFGEDRFRWSATMSGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA077/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA077/annotation12/range/1

https://synbiohub.org/public/SEGA/SEGA077/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA077/1
start: 2420
end: 3610
orientation: inline

L3S2P11 Terminator
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
label: L3S2P11 Terminator
note: L3S2P11 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA077/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA077/annotation1/range/1

https://synbiohub.org/public/SEGA/SEGA077/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA077/1
start: 538
end: 594
orientation: inline

Promoter 3BCD2
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
label: Promoter 3BCD2
note: Promoter 3BCD2
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA077/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA077/annotation11/range/1

https://synbiohub.org/public/SEGA/SEGA077/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA077/1
start: 2352
end: 2380
orientation: inline

TetR
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
label: TetR
note: Geneious type: CDS
note: TetR
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA077/1
role: SO:0001411
location: https://synbiohub.org/public/SEGA/SEGA077/annotation2/range/1

https://synbiohub.org/public/SEGA/SEGA077/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA077/1
start: 611
end: 1234
orientation: reverseComplement

pLacI
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
label: pLacI
note: lacI promoter
note: pLacI
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA077/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA077/annotation4/range/1

https://synbiohub.org/public/SEGA/SEGA077/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA077/1
start: 1263
end: 1316
orientation: reverseComplement

tet1
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
label: tet1
note: tet1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA077/1
role: SO:0000139
location: https://synbiohub.org/public/SEGA/SEGA077/annotation3/range/1

https://synbiohub.org/public/SEGA/SEGA077/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA077/1
start: 1235
end: 1262
orientation: inline

pstS
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
codon start: 1
label: pstS
note: pstS
translation: MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKWADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA077/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA077/annotation14/range/1

https://synbiohub.org/public/SEGA/SEGA077/annotation14/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA077/1
start: 3856
end: 4103
orientation: inline

pTet*
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
label: pTet*
note: pTet*
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA077/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA077/annotation5/range/1

https://synbiohub.org/public/SEGA/SEGA077/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA077/1
start: 1343
end: 1418
orientation: inline

https://synbiohub.org/public/SEGA/SEGA070/1
type: ComponentDefinition
persistent identity: SEGA070
display id: SEGA070
version: 1
description: natural linear DNA.
owned by: carbay
owned by: morno
date: 08-SEP-2021
division: UNA
keywords: .
locus: SEGA070
molecule: ds-DNA
organism: unspecified
reference: https://synbiohub.org/user/carbay/SEGA/SEGA070_reference0/1
source: natural DNA sequence
type: DnaRegion
type: SO:0000987
role: SO:0000804
sequence annotation: L3S3P22 Terminator (synthetic)
sequence annotation: glmS
sequence annotation: TcR
sequence annotation: L3S2P11 Terminator
sequence annotation: lacI promoter
sequence annotation: rrnC
sequence annotation: lacI
sequence annotation: sfGFP
sequence annotation: pstS
sequence annotation: Promoter 3BCD2
sequence annotation: high TIR
sequence annotation: T7 promoter
sequence annotation: pelB signal sequence
sequence annotation: lac operator
sequence: https://synbiohub.org/public/SEGA/SEGA070_seq/1

L3S3P22 Terminator (synthetic)
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
label: L3S3P22 Terminator (synthetic)
note: L3S3P22 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA070/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA070/annotation9/range/1

https://synbiohub.org/public/SEGA/SEGA070/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA070/1
start: 2648
end: 2696
orientation: inline

glmS
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
codon start: 1
label: glmS
note: glmS
translation: VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA070/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA070/annotation0/range/1

https://synbiohub.org/public/SEGA/SEGA070/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA070/1
start: 1
end: 528
orientation: inline

TcR
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
codon start: 1
gene: tet
label: TcR
note: TcR
note: confers resistance to tetracycline
product: tetracycline efflux protein
translation: MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHYGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVIFGEDRFRWSATMSGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA070/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA070/annotation11/range/1

https://synbiohub.org/public/SEGA/SEGA070/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA070/1
start: 2775
end: 3965
orientation: inline

L3S2P11 Terminator
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
label: L3S2P11 Terminator
note: L3S2P11 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA070/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA070/annotation1/range/1

https://synbiohub.org/public/SEGA/SEGA070/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA070/1
start: 538
end: 594
orientation: inline

lacI promoter
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
gene: lacI
label: lacI promoter
note: lacI promoter
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA070/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA070/annotation3/range/1

https://synbiohub.org/public/SEGA/SEGA070/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA070/1
start: 1687
end: 1764
orientation: reverseComplement

rrnC
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
label: rrnC
note: rrnC
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA070/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA070/annotation12/range/1

https://synbiohub.org/public/SEGA/SEGA070/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA070/1
start: 3976
end: 4016
orientation: inline

lacI
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
codon start: 1
gene: lacI
label: lacI
note: The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG).
note: lacI
product: lac repressor
translation: MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA070/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA070/annotation2/range/1

https://synbiohub.org/public/SEGA/SEGA070/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA070/1
start: 604
end: 1686
orientation: reverseComplement

sfGFP
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
codon start: 1
label: sfGFP
note: sfGFP
product: superfolder GFP
translation: RKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDGTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEFVTAAGITHGMDELYK
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA070/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA070/annotation8/range/1

https://synbiohub.org/public/SEGA/SEGA070/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA070/1
start: 1934
end: 2644
orientation: inline

pstS
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
codon start: 1
label: pstS
note: pstS
translation: MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKWADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA070/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA070/annotation13/range/1

https://synbiohub.org/public/SEGA/SEGA070/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA070/1
start: 4211
end: 4458
orientation: inline

Promoter 3BCD2
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
label: Promoter 3BCD2
note: Promoter 3BCD2
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA070/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA070/annotation10/range/1

https://synbiohub.org/public/SEGA/SEGA070/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA070/1
start: 2707
end: 2735
orientation: inline

high TIR
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
note: TIR
note: high TIR
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA070/1
role: SO:0000139
location: https://synbiohub.org/public/SEGA/SEGA070/annotation6/range/1

https://synbiohub.org/public/SEGA/SEGA070/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA070/1
start: 1850
end: 1876
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
label: T7 promoter
note: T7 promoter
note: promoter for bacteriophage T7 RNA polymerase
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA070/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA070/annotation4/range/1

https://synbiohub.org/public/SEGA/SEGA070/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA070/1
start: 1795
end: 1813
orientation: inline

pelB signal sequence
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
codon start: 1
gene: pelB (fragment)
label: pelB signal sequence
note: pelB signal sequence
product: leader peptide for secretion
translation: MKYLLPTAAAGLLLLAAQPAMA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA070/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA070/annotation7/range/1

https://synbiohub.org/public/SEGA/SEGA070/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA070/1
start: 1868
end: 1933
orientation: inline

lac operator
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
bound moiety: lac repressor encoded by lacI
feature type: protein_bind
label: lac operator
note: The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG).
note: lac operator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA070/1
role: SO:0000110
location: https://synbiohub.org/public/SEGA/SEGA070/annotation5/range/1

https://synbiohub.org/public/SEGA/SEGA070/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA070/1
start: 1814
end: 1838
orientation: inline

https://synbiohub.org/public/SEGA/SEGA038/1
type: ComponentDefinition
persistent identity: SEGA038
display id: SEGA038
version: 1
description: natural linear DNA.
owned by: carbay
owned by: morno
date: 09-SEP-2021
division: UNA
keywords: .
locus: SEGA038
molecule: ds-DNA
organism: unspecified
reference: https://synbiohub.org/user/carbay/SEGA/SEGA038_reference0/1
source: natural DNA sequence
type: DnaRegion
type: SO:0000987
role: SO:0000804
sequence annotation: rhaS
sequence annotation: glmS
sequence annotation: Promoter 3BCD2
sequence annotation: L3S2P11 Terminator
sequence annotation: rhaR
sequence annotation: low TIR
sequence annotation: BCD
sequence annotation: pstS
sequence annotation: GFP
sequence annotation: rrnC
sequence annotation: rhaB promoter
sequence annotation: TcR
sequence annotation: L3S3P22 Terminator (synthetic)
sequence: https://synbiohub.org/public/SEGA/SEGA038_seq/1

rhaS
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
codon start: 1
gene: E. coli rhaS
label: rhaS
note: activated by L-rhamnose
note: rhaS
product: positive regulator of the rhaB promoter
translation: MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHGTGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLLPQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKSSLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQRYLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGFLQ
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA038/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA038/annotation3/range/1

https://synbiohub.org/public/SEGA/SEGA038/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA038/1
start: 1526
end: 2362
orientation: reverseComplement

glmS
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
codon start: 1
label: glmS
note: glmS
translation: VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA038/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA038/annotation0/range/1

https://synbiohub.org/public/SEGA/SEGA038/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA038/1
start: 1
end: 528
orientation: inline

Promoter 3BCD2
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
label: Promoter 3BCD2
note: Promoter 3BCD2
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA038/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA038/annotation9/range/1

https://synbiohub.org/public/SEGA/SEGA038/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA038/1
start: 3485
end: 3513
orientation: inline

L3S2P11 Terminator
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
label: L3S2P11 Terminator
note: L3S2P11 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA038/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA038/annotation1/range/1

https://synbiohub.org/public/SEGA/SEGA038/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA038/1
start: 538
end: 594
orientation: inline

rhaR
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
codon start: 1
gene: E. coli rhaR
label: rhaR
note: needed for rhamnose-dependent activation of the rhaB promoter
note: rhaR
product: transcriptional activator of rhaR and rhaS
translation: MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQGAIPGFNASAGQPHWRLGSMGMAQARQVIGQLEHESSQHVPFANEMAELLFGQLVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRHLNSQKD
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA038/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA038/annotation2/range/1

https://synbiohub.org/public/SEGA/SEGA038/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA038/1
start: 607
end: 1452
orientation: reverseComplement

low TIR
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
note: TIR
note: low TIR
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA038/1
role: SO:0000139
location: https://synbiohub.org/public/SEGA/SEGA038/annotation5/range/1

https://synbiohub.org/public/SEGA/SEGA038/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA038/1
start: 2638
end: 2664
orientation: inline

BCD
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
codon start: 1
label: BCD
note: BCD
translation: MKAIFVLKHLNHAKEVF
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA038/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA038/annotation6/range/1

https://synbiohub.org/public/SEGA/SEGA038/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA038/1
start: 2656
end: 2711
orientation: inline

pstS
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
codon start: 1
label: pstS
note: pstS
translation: MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKWADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA038/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA038/annotation12/range/1

https://synbiohub.org/public/SEGA/SEGA038/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA038/1
start: 4989
end: 5236
orientation: inline

GFP
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
codon start: 1
label: GFP
note: GFP
product: green fluorescent protein
translation: SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYK
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA038/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA038/annotation7/range/1

https://synbiohub.org/public/SEGA/SEGA038/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA038/1
start: 2712
end: 3422
orientation: inline

rrnC
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
label: rrnC
note: rrnC
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA038/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA038/annotation11/range/1

https://synbiohub.org/public/SEGA/SEGA038/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA038/1
start: 4754
end: 4794
orientation: inline

rhaB promoter
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
label: rhaB promoter
note: promoter of the E. coli rhaBAD operon, conferring tight induction with L-rhamnose and repression with D-glucose in the presence of RhaR and RhaS (Giacalone et al., 2006)
note: rhaB promoter
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA038/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA038/annotation4/range/1

https://synbiohub.org/public/SEGA/SEGA038/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA038/1
start: 2508
end: 2626
orientation: inline

TcR
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
codon start: 1
gene: tet
label: TcR
note: TcR
note: confers resistance to tetracycline
product: tetracycline efflux protein
translation: MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHYGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVIFGEDRFRWSATMSGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA038/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA038/annotation10/range/1

https://synbiohub.org/public/SEGA/SEGA038/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA038/1
start: 3553
end: 4743
orientation: inline

L3S3P22 Terminator (synthetic)
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
label: L3S3P22 Terminator (synthetic)
note: L3S3P22 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA038/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA038/annotation8/range/1

https://synbiohub.org/public/SEGA/SEGA038/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA038/1
start: 3426
end: 3474
orientation: inline

https://synbiohub.org/public/SEGA/SEGA066/1
type: ComponentDefinition
persistent identity: SEGA066
display id: SEGA066
version: 1
description: natural linear DNA.
owned by: carbay
owned by: morno
date: 08-SEP-2021
division: UNA
keywords: .
locus: SEGA066
molecule: ds-DNA
organism: unspecified
reference: https://synbiohub.org/user/carbay/SEGA/SEGA066_reference0/1
source: natural DNA sequence
type: DnaRegion
type: SO:0000987
role: SO:0000804
sequence annotation: PROTi-Linker
sequence annotation: pstS
sequence annotation: Promoter 3BCD2
sequence annotation: lac operator
sequence annotation: TcR
sequence annotation: lacI promoter
sequence annotation: lacI
sequence annotation: TEV site
sequence annotation: L3S3P22 Terminator (synthetic)
sequence annotation: rrnC
sequence annotation: glmS
sequence annotation: T7 promoter
sequence annotation: GFP
sequence annotation: medium TIR
sequence annotation: L3S2P11 Terminator
sequence: https://synbiohub.org/public/SEGA/SEGA066_seq/1

PROTi-Linker
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
label: PROTi-Linker
note: PROTi-Linker
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA066/1
role: SO:0001411
location: https://synbiohub.org/public/SEGA/SEGA066/annotation8/range/1

https://synbiohub.org/public/SEGA/SEGA066/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA066/1
start: 1889
end: 1924
orientation: inline

pstS
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
codon start: 1
label: pstS
note: pstS
translation: MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKWADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA066/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA066/annotation14/range/1

https://synbiohub.org/public/SEGA/SEGA066/annotation14/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA066/1
start: 4202
end: 4449
orientation: inline

Promoter 3BCD2
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
label: Promoter 3BCD2
note: Promoter 3BCD2
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA066/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA066/annotation11/range/1

https://synbiohub.org/public/SEGA/SEGA066/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA066/1
start: 2698
end: 2726
orientation: inline

lac operator
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
bound moiety: lac repressor encoded by lacI
feature type: protein_bind
label: lac operator
note: The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG).
note: lac operator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA066/1
role: SO:0000110
location: https://synbiohub.org/public/SEGA/SEGA066/annotation5/range/1

https://synbiohub.org/public/SEGA/SEGA066/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA066/1
start: 1814
end: 1838
orientation: inline

TcR
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
codon start: 1
gene: tet
label: TcR
note: TcR
note: confers resistance to tetracycline
product: tetracycline efflux protein
translation: MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHYGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVIFGEDRFRWSATMSGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA066/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA066/annotation12/range/1

https://synbiohub.org/public/SEGA/SEGA066/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA066/1
start: 2766
end: 3956
orientation: inline

lacI promoter
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
gene: lacI
label: lacI promoter
note: lacI promoter
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA066/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA066/annotation3/range/1

https://synbiohub.org/public/SEGA/SEGA066/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA066/1
start: 1687
end: 1764
orientation: reverseComplement

lacI
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
codon start: 1
gene: lacI
label: lacI
note: The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG).
note: lacI
product: lac repressor
translation: MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA066/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA066/annotation2/range/1

https://synbiohub.org/public/SEGA/SEGA066/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA066/1
start: 604
end: 1686
orientation: reverseComplement

TEV site
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
codon start: 1
label: TEV site
note: TEV site
product: tobacco etch virus (TEV) protease recognition and cleavage site
translation: MENLYFQ
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA066/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA066/annotation7/range/1

https://synbiohub.org/public/SEGA/SEGA066/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA066/1
start: 1868
end: 1888
orientation: inline

L3S3P22 Terminator (synthetic)
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
label: L3S3P22 Terminator (synthetic)
note: L3S3P22 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA066/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA066/annotation10/range/1

https://synbiohub.org/public/SEGA/SEGA066/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA066/1
start: 2639
end: 2687
orientation: inline

rrnC
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
label: rrnC
note: rrnC
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA066/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA066/annotation13/range/1

https://synbiohub.org/public/SEGA/SEGA066/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA066/1
start: 3967
end: 4007
orientation: inline

glmS
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
codon start: 1
label: glmS
note: glmS
translation: VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA066/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA066/annotation0/range/1

https://synbiohub.org/public/SEGA/SEGA066/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA066/1
start: 1
end: 528
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
label: T7 promoter
note: T7 promoter
note: promoter for bacteriophage T7 RNA polymerase
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA066/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA066/annotation4/range/1

https://synbiohub.org/public/SEGA/SEGA066/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA066/1
start: 1795
end: 1813
orientation: inline

GFP
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
codon start: 1
label: GFP
note: GFP
product: green fluorescent protein
translation: SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYK
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA066/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA066/annotation9/range/1

https://synbiohub.org/public/SEGA/SEGA066/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA066/1
start: 1925
end: 2635
orientation: inline

medium TIR
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
note: TIR
note: medium TIR
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA066/1
role: SO:0000139
location: https://synbiohub.org/public/SEGA/SEGA066/annotation6/range/1

https://synbiohub.org/public/SEGA/SEGA066/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA066/1
start: 1850
end: 1876
orientation: inline

L3S2P11 Terminator
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
label: L3S2P11 Terminator
note: L3S2P11 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA066/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA066/annotation1/range/1

https://synbiohub.org/public/SEGA/SEGA066/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA066/1
start: 538
end: 594
orientation: inline

https://synbiohub.org/public/SEGA/SEGA080/1
type: ComponentDefinition
persistent identity: SEGA080
display id: SEGA080
version: 1
description: natural linear DNA.
owned by: carbay
owned by: morno
date: 09-SEP-2021
division: UNA
keywords: .
locus: SEGA080
molecule: ds-DNA
organism: unspecified
reference: https://synbiohub.org/public/SEGA/reference0_1/1
source: natural DNA sequence
type: DnaRegion
type: SO:0000987
role: SO:0000804
sequence annotation: Terminator rrnB T1
sequence annotation: rrnC
sequence annotation: glmS
sequence annotation: rhaB promoter
sequence annotation: high TIR
sequence annotation: EM7 promoter
sequence annotation: L3S3P22
sequence annotation: galK
sequence annotation: rhaR
sequence annotation: Promoter 3BCD2
sequence annotation: rhaS
sequence annotation: pstS
sequence annotation: TcR
sequence annotation: BCD
sequence annotation: L3S2P11 Terminator
sequence annotation: GFP
sequence: https://synbiohub.org/public/SEGA/SEGA080_seq/1

Terminator rrnB T1
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
label: Terminator rrnB T1
label: rrnB
note: rrnB
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA080/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA080/annotation4/range/1

https://synbiohub.org/public/SEGA/SEGA080/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA080/1
start: 1843
end: 1886
orientation: inline

rrnC
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
label: rrnC
note: rrnC
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA080/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA080/annotation14/range/1

https://synbiohub.org/public/SEGA/SEGA080/annotation14/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA080/1
start: 6046
end: 6086
orientation: inline

glmS
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
codon start: 1
label: glmS
note: glmS
translation: VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA080/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA080/annotation0/range/1

https://synbiohub.org/public/SEGA/SEGA080/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA080/1
start: 1
end: 528
orientation: inline

rhaB promoter
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
label: rhaB promoter
note: promoter of the E. coli rhaBAD operon, conferring tight induction with L-rhamnose and repression with D-glucose in the presence of RhaR and RhaS (Giacalone et al., 2006)
note: rhaB promoter
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA080/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA080/annotation7/range/1

https://synbiohub.org/public/SEGA/SEGA080/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA080/1
start: 3800
end: 3918
orientation: inline

high TIR
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
note: TIR
note: high TIR
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA080/1
role: SO:0000139
location: https://synbiohub.org/public/SEGA/SEGA080/annotation8/range/1

https://synbiohub.org/public/SEGA/SEGA080/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA080/1
start: 3930
end: 3956
orientation: inline

EM7 promoter
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
label: EM7 promoter
note: EM7 promoter
note: synthetic bacterial promoter
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA080/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA080/annotation3/range/1

https://synbiohub.org/public/SEGA/SEGA080/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA080/1
start: 1783
end: 1830
orientation: reverseComplement

L3S3P22
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
label: L3S3P22
note: L3S3P22
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA080/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA080/annotation11/range/1

https://synbiohub.org/public/SEGA/SEGA080/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA080/1
start: 4718
end: 4766
orientation: inline

galK
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
EC number: 2.7.1.6
codon start: 1
db xref: ASAP:ABE-0002568
db xref: EcoGene:EG10363
db xref: GI:16128725
db xref: GeneID:945358
db xref: UniProtKB/Swiss-Prot:P0A6T3
function: enzyme; Degradation of small molecules: Carbon compounds
gene: galK
gene synonym: ECK0746; galA; JW0740
label: galK
locus tag: b0757
note: galK
product: galactokinase
protein id: NP_415278.1
translation: MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVLPCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLDAPIVAHENYQWANYVRGVVKHLQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQEAENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTLVGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTVEAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGGGFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETLYVCKPSQGAGQC
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA080/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA080/annotation2/range/1

https://synbiohub.org/public/SEGA/SEGA080/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA080/1
start: 605
end: 1753
orientation: reverseComplement

rhaR
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
codon start: 1
gene: E. coli rhaR
label: rhaR
note: needed for rhamnose-dependent activation of the rhaB promoter
note: rhaR
product: transcriptional activator of rhaR and rhaS
translation: MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQGAIPGFNASAGQPHWRLGSMGMAQARQVIGQLEHESSQHVPFANEMAELLFGQLVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRHLNSQKD
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA080/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA080/annotation5/range/1

https://synbiohub.org/public/SEGA/SEGA080/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA080/1
start: 1899
end: 2744
orientation: reverseComplement

Promoter 3BCD2
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
label: Promoter 3BCD2
note: Promoter 3BCD2
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA080/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA080/annotation12/range/1

https://synbiohub.org/public/SEGA/SEGA080/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA080/1
start: 4777
end: 4805
orientation: inline

rhaS
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
codon start: 1
gene: E. coli rhaS
label: rhaS
note: activated by L-rhamnose
note: rhaS
product: positive regulator of the rhaB promoter
translation: MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHGTGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLLPQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKSSLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQRYLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGFLQ
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA080/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA080/annotation6/range/1

https://synbiohub.org/public/SEGA/SEGA080/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA080/1
start: 2818
end: 3654
orientation: reverseComplement

pstS
type: SequenceAnnotation
persistent identity: annotation15
display id: annotation15
version: 1
codon start: 1
label: pstS
note: pstS
translation: MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKWADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA080/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA080/annotation15/range/1

https://synbiohub.org/public/SEGA/SEGA080/annotation15/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA080/1
start: 6281
end: 6528
orientation: inline

TcR
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
codon start: 1
gene: tet
label: TcR
note: TcR
note: confers resistance to tetracycline
product: tetracycline efflux protein
translation: MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHYGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVIFGEDRFRWSATMSGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA080/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA080/annotation13/range/1

https://synbiohub.org/public/SEGA/SEGA080/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA080/1
start: 4845
end: 6035
orientation: inline

BCD
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
codon start: 1
label: BCD
note: BCD
translation: MKAIFVLKHLNHAKEVF
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA080/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA080/annotation9/range/1

https://synbiohub.org/public/SEGA/SEGA080/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA080/1
start: 3948
end: 4003
orientation: inline

L3S2P11 Terminator
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
label: L3S2P11 Terminator
note: L3S2P11 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA080/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA080/annotation1/range/1

https://synbiohub.org/public/SEGA/SEGA080/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA080/1
start: 538
end: 594
orientation: inline

GFP
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
codon start: 1
label: GFP
note: GFP
product: gree fluorescent protein
translation: SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYK
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA080/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA080/annotation10/range/1

https://synbiohub.org/public/SEGA/SEGA080/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA080/1
start: 4004
end: 4714
orientation: inline

https://synbiohub.org/public/SEGA/SEGA044/1
type: ComponentDefinition
persistent identity: SEGA044
display id: SEGA044
version: 1
description: natural linear DNA.
owned by: carbay
owned by: morno
date: 08-SEP-2021
division: UNA
keywords: .
locus: SEGA044
molecule: ds-DNA
organism: unspecified
reference: https://synbiohub.org/user/carbay/SEGA/SEGA044_reference0/1
source: natural DNA sequence
type: DnaRegion
type: SO:0000987
role: SO:0000804
sequence annotation: L3S2P11 Terminator
sequence annotation: rhaS
sequence annotation: rrnC
sequence annotation: TcR
sequence annotation: rhaB promoter
sequence annotation: pstS
sequence annotation: glmS
sequence annotation: Promoter 3BCD2
sequence annotation: GFP
sequence annotation: low TIR
sequence annotation: L3S3P22 Terminator (synthetic)
sequence annotation: TEV site
sequence annotation: rhaR
sequence: https://synbiohub.org/public/SEGA/SEGA044_seq/1

L3S2P11 Terminator
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
label: L3S2P11 Terminator
note: L3S2P11 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA044/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA044/annotation1/range/1

https://synbiohub.org/public/SEGA/SEGA044/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA044/1
start: 538
end: 594
orientation: inline

rhaS
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
codon start: 1
gene: E. coli rhaS
label: rhaS
note: activated by L-rhamnose
note: rhaS
product: positive regulator of the rhaB promoter
translation: MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHGTGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLLPQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKSSLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQRYLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGFLQ
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA044/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA044/annotation3/range/1

https://synbiohub.org/public/SEGA/SEGA044/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA044/1
start: 1526
end: 2362
orientation: reverseComplement

rrnC
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
label: rrnC
note: rrnC
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA044/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA044/annotation11/range/1

https://synbiohub.org/public/SEGA/SEGA044/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA044/1
start: 4719
end: 4759
orientation: inline

TcR
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
codon start: 1
gene: tet
label: TcR
note: TcR
note: confers resistance to tetracycline
product: tetracycline efflux protein
translation: MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHYGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVIFGEDRFRWSATMSGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA044/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA044/annotation10/range/1

https://synbiohub.org/public/SEGA/SEGA044/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA044/1
start: 3518
end: 4708
orientation: inline

rhaB promoter
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
label: rhaB promoter
note: promoter of the E. coli rhaBAD operon, conferring tight induction with L-rhamnose and repression with D-glucose in the presence of RhaR and RhaS (Giacalone et al., 2006)
note: rhaB promoter
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA044/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA044/annotation4/range/1

https://synbiohub.org/public/SEGA/SEGA044/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA044/1
start: 2508
end: 2626
orientation: inline

pstS
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
codon start: 1
label: pstS
note: pstS
translation: MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKWADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA044/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA044/annotation12/range/1

https://synbiohub.org/public/SEGA/SEGA044/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA044/1
start: 4954
end: 5201
orientation: inline

glmS
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
codon start: 1
label: glmS
note: glmS
translation: VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA044/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA044/annotation0/range/1

https://synbiohub.org/public/SEGA/SEGA044/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA044/1
start: 1
end: 528
orientation: inline

Promoter 3BCD2
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
label: Promoter 3BCD2
note: Promoter 3BCD2
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA044/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA044/annotation9/range/1

https://synbiohub.org/public/SEGA/SEGA044/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA044/1
start: 3450
end: 3478
orientation: inline

GFP
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
codon start: 1
label: GFP
note: GFP
product: green fluorescent protein
translation: SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYK
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA044/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA044/annotation7/range/1

https://synbiohub.org/public/SEGA/SEGA044/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA044/1
start: 2677
end: 3387
orientation: inline

low TIR
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
note: TIR
note: low TIR
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA044/1
role: SO:0000139
location: https://synbiohub.org/public/SEGA/SEGA044/annotation5/range/1

https://synbiohub.org/public/SEGA/SEGA044/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA044/1
start: 2638
end: 2664
orientation: inline

L3S3P22 Terminator (synthetic)
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
label: L3S3P22 Terminator (synthetic)
note: L3S3P22 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA044/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA044/annotation8/range/1

https://synbiohub.org/public/SEGA/SEGA044/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA044/1
start: 3391
end: 3439
orientation: inline

TEV site
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
codon start: 1
label: TEV site
note: TEV site
product: tobacco etch virus (TEV) protease recognition and cleavage site
translation: MENLYFQ
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA044/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA044/annotation6/range/1

https://synbiohub.org/public/SEGA/SEGA044/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA044/1
start: 2656
end: 2676
orientation: inline

rhaR
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
codon start: 1
gene: E. coli rhaR
label: rhaR
note: needed for rhamnose-dependent activation of the rhaB promoter
note: rhaR
product: transcriptional activator of rhaR and rhaS
translation: MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQGAIPGFNASAGQPHWRLGSMGMAQARQVIGQLEHESSQHVPFANEMAELLFGQLVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRHLNSQKD
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA044/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA044/annotation2/range/1

https://synbiohub.org/public/SEGA/SEGA044/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA044/1
start: 607
end: 1452
orientation: reverseComplement

https://synbiohub.org/public/SEGA/SEGA035/1
type: ComponentDefinition
persistent identity: SEGA035
display id: SEGA035
version: 1
description: natural linear DNA.
owned by: carbay
owned by: morno
date: 09-SEP-2021
division: UNA
keywords: .
locus: SEGA035
molecule: ds-DNA
organism: unspecified
reference: https://synbiohub.org/user/carbay/SEGA/SEGA035_reference0/1
source: natural DNA sequence
type: DnaRegion
type: SO:0000987
role: SO:0000804
sequence annotation: Promoter 3BCD2
sequence annotation: YebF
sequence annotation: lacI
sequence annotation: trc promoter
sequence annotation: pstS
sequence annotation: sfGFP
sequence annotation: L3S3P22 Terminator (synthetic)
sequence annotation: lacI promoter
sequence annotation: glmS
sequence annotation: YebFss
sequence annotation: TcR
sequence annotation: lacO
sequence annotation: L3S2P11 Terminator
sequence annotation: low TIR
sequence annotation: rrnC
sequence: https://synbiohub.org/public/SEGA/SEGA035_seq/1

Promoter 3BCD2
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
label: Promoter 3BCD2
note: Promoter 3BCD2
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA035/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA035/annotation11/range/1

https://synbiohub.org/public/SEGA/SEGA035/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA035/1
start: 3005
end: 3033
orientation: inline

YebF
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
codon start: 1
label: YebF
note: YebF
product: full size yebF protein
translation: ANNETSKSVTFPKCEDLDAAGIAASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHYQVSVDCKAGMAEYQRR
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA035/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA035/annotation8/range/1

https://synbiohub.org/public/SEGA/SEGA035/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA035/1
start: 1941
end: 2231
orientation: inline

lacI
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
codon start: 1
gene: lacI
label: lacI
note: The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG).
note: lacI
product: lac repressor
translation: MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA035/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA035/annotation2/range/1

https://synbiohub.org/public/SEGA/SEGA035/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA035/1
start: 604
end: 1686
orientation: reverseComplement

trc promoter
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
label: trc promoter
note: strong E. coli promoter; hybrid between the trp and lac UV5 promoters
note: trc promoter
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA035/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA035/annotation4/range/1

https://synbiohub.org/public/SEGA/SEGA035/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA035/1
start: 1795
end: 1824
orientation: inline

pstS
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
codon start: 1
label: pstS
note: pstS
translation: MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKWADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA035/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA035/annotation14/range/1

https://synbiohub.org/public/SEGA/SEGA035/annotation14/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA035/1
start: 4509
end: 4756
orientation: inline

sfGFP
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
codon start: 1
label: sfGFP
note: sfGFP
product: superfolder GFP
translation: RKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDGTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEFVTAAGITHGMDELYK
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA035/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA035/annotation9/range/1

https://synbiohub.org/public/SEGA/SEGA035/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA035/1
start: 2232
end: 2942
orientation: inline

L3S3P22 Terminator (synthetic)
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
label: L3S3P22 Terminator (synthetic)
note: L3S3P22 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA035/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA035/annotation10/range/1

https://synbiohub.org/public/SEGA/SEGA035/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA035/1
start: 2946
end: 2994
orientation: inline

lacI promoter
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
gene: lacI
label: lacI promoter
note: lacI promoter
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA035/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA035/annotation3/range/1

https://synbiohub.org/public/SEGA/SEGA035/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA035/1
start: 1687
end: 1764
orientation: reverseComplement

glmS
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
codon start: 1
label: glmS
note: glmS
translation: VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA035/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA035/annotation0/range/1

https://synbiohub.org/public/SEGA/SEGA035/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA035/1
start: 1
end: 528
orientation: inline

YebFss
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
codon start: 1
db xref: ASAP:ABE-0006141
db xref: EcoGene:EG11807
db xref: GI:145698278
db xref: GeneID:946363
db xref: UniProtKB/Swiss-Prot:P33219
gene: yebF
gene synonym: ECK1848; JW1836
label: YebFss
locus tag: b1847
note: YebFss
product: yebF signal sequence
protein id: NP_416361.2
translation: MKKRGAFLGLLLVSACASVFA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA035/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA035/annotation7/range/1

https://synbiohub.org/public/SEGA/SEGA035/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA035/1
start: 1878
end: 1940
orientation: inline

TcR
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
codon start: 1
gene: tet
label: TcR
note: TcR
note: confers resistance to tetracycline
product: tetracycline efflux protein
translation: MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHYGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVIFGEDRFRWSATMSGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA035/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA035/annotation12/range/1

https://synbiohub.org/public/SEGA/SEGA035/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA035/1
start: 3073
end: 4263
orientation: inline

lacO
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
bound moiety: lac repressor
feature type: protein_bind
label: lacO
note: lac operator
note: lacO
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA035/1
role: SO:0000110
location: https://synbiohub.org/public/SEGA/SEGA035/annotation5/range/1

https://synbiohub.org/public/SEGA/SEGA035/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA035/1
start: 1825
end: 1848
orientation: inline

L3S2P11 Terminator
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
label: L3S2P11 Terminator
note: L3S2P11 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA035/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA035/annotation1/range/1

https://synbiohub.org/public/SEGA/SEGA035/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA035/1
start: 538
end: 594
orientation: inline

low TIR
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
note: TIR
note: low TIR
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA035/1
role: SO:0000139
location: https://synbiohub.org/public/SEGA/SEGA035/annotation6/range/1

https://synbiohub.org/public/SEGA/SEGA035/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA035/1
start: 1860
end: 1886
orientation: inline

rrnC
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
label: rrnC
note: rrnC
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA035/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA035/annotation13/range/1

https://synbiohub.org/public/SEGA/SEGA035/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA035/1
start: 4274
end: 4314
orientation: inline

https://synbiohub.org/public/SEGA/SEGA085/1
type: ComponentDefinition
persistent identity: SEGA085
display id: SEGA085
version: 1
description: natural linear DNA.
owned by: carbay
owned by: morno
date: 09-SEP-2021
division: UNA
keywords: .
locus: SEGA085
molecule: ds-DNA
organism: unspecified
reference: https://synbiohub.org/user/carbay/SEGA/SEGA085_reference0/1
source: natural DNA sequence
type: DnaRegion
type: SO:0000987
role: SO:0000804
sequence annotation: EM7 promoter
sequence annotation: rhaR
sequence annotation: Terminator rrnB T1
sequence annotation: L3S2P11 Terminator
sequence annotation: galK
sequence annotation: L3S3P22 Terminator (synthetic)
sequence annotation: medium TIR
sequence annotation: pstS
sequence annotation: glmS
sequence annotation: GFP
sequence annotation: TcR
sequence annotation: rhaB promoter
sequence annotation: TEV site
sequence annotation: rrnC
sequence annotation: rhaS
sequence annotation: Promoter 3BCD2
sequence: https://synbiohub.org/public/SEGA/SEGA085_seq/1

EM7 promoter
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
label: EM7 promoter
note: EM7 promoter
note: synthetic bacterial promoter
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA085/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA085/annotation3/range/1

https://synbiohub.org/public/SEGA/SEGA085/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA085/1
start: 1783
end: 1830
orientation: reverseComplement

rhaR
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
codon start: 1
gene: E. coli rhaR
label: rhaR
note: needed for rhamnose-dependent activation of the rhaB promoter
note: rhaR
product: transcriptional activator of rhaR and rhaS
translation: MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQGAIPGFNASAGQPHWRLGSMGMAQARQVIGQLEHESSQHVPFANEMAELLFGQLVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRHLNSQKD
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA085/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA085/annotation5/range/1

https://synbiohub.org/public/SEGA/SEGA085/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA085/1
start: 1899
end: 2744
orientation: reverseComplement

Terminator rrnB T1
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
label: Terminator rrnB T1
note: Terminator rrnB T1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA085/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA085/annotation4/range/1

https://synbiohub.org/public/SEGA/SEGA085/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA085/1
start: 1843
end: 1886
orientation: inline

L3S2P11 Terminator
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
label: L3S2P11 Terminator
note: L3S2P11 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA085/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA085/annotation1/range/1

https://synbiohub.org/public/SEGA/SEGA085/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA085/1
start: 538
end: 594
orientation: inline

galK
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
EC number: 2.7.1.6
codon start: 1
db xref: ASAP:ABE-0002568
db xref: EcoGene:EG10363
db xref: GI:16128725
db xref: GeneID:945358
db xref: UniProtKB/Swiss-Prot:P0A6T3
function: enzyme; Degradation of small molecules: Carbon compounds
gene: galK
gene synonym: ECK0746; galA; JW0740
label: galK
locus tag: b0757
note: galK
product: galactokinase
protein id: NP_415278.1
translation: MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVLPCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLDAPIVAHENYQWANYVRGVVKHLQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQEAENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTLVGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTVEAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGGGFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA085/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA085/annotation2/range/1

https://synbiohub.org/public/SEGA/SEGA085/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA085/1
start: 605
end: 1753
orientation: reverseComplement

L3S3P22 Terminator (synthetic)
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
label: L3S3P22 Terminator (synthetic)
note: L3S3P22 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA085/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA085/annotation11/range/1

https://synbiohub.org/public/SEGA/SEGA085/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA085/1
start: 4683
end: 4731
orientation: inline

medium TIR
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
note: TIR
note: medium TIR
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA085/1
role: SO:0000139
location: https://synbiohub.org/public/SEGA/SEGA085/annotation8/range/1

https://synbiohub.org/public/SEGA/SEGA085/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA085/1
start: 3930
end: 3956
orientation: inline

pstS
type: SequenceAnnotation
persistent identity: annotation15
display id: annotation15
version: 1
codon start: 1
label: pstS
note: pstS
translation: MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKWADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA085/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA085/annotation15/range/1

https://synbiohub.org/public/SEGA/SEGA085/annotation15/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA085/1
start: 6246
end: 6493
orientation: inline

glmS
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
codon start: 1
label: glmS
note: glmS
translation: VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA085/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA085/annotation0/range/1

https://synbiohub.org/public/SEGA/SEGA085/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA085/1
start: 1
end: 528
orientation: inline

GFP
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
codon start: 1
label: GFP
note: GFP
product: green fluorescent protein
translation: SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYK
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA085/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA085/annotation10/range/1

https://synbiohub.org/public/SEGA/SEGA085/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA085/1
start: 3969
end: 4679
orientation: inline

TcR
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
codon start: 1
gene: tet
label: TcR
note: TcR
note: confers resistance to tetracycline
product: tetracycline efflux protein
translation: MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHYGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA085/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA085/annotation13/range/1

https://synbiohub.org/public/SEGA/SEGA085/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA085/1
start: 4810
end: 6000
orientation: inline

rhaB promoter
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
label: rhaB promoter
note: promoter of the E. coli rhaBAD operon, conferring tight induction with L-rhamnose and repression with D-glucose in the presence of RhaR and RhaS (Giacalone et al., 2006)
note: rhaB promoter
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA085/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA085/annotation7/range/1

https://synbiohub.org/public/SEGA/SEGA085/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA085/1
start: 3800
end: 3918
orientation: inline

TEV site
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
codon start: 1
label: TEV site
note: TEV site
product: tobacco etch virus (TEV) protease recognition and cleavage site
translation: MENLYFQ
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA085/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA085/annotation9/range/1

https://synbiohub.org/public/SEGA/SEGA085/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA085/1
start: 3948
end: 3968
orientation: inline

rrnC
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
label: rrnC
note: rrnC
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA085/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA085/annotation14/range/1

https://synbiohub.org/public/SEGA/SEGA085/annotation14/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA085/1
start: 6011
end: 6051
orientation: inline

rhaS
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
codon start: 1
gene: E. coli rhaS
label: rhaS
note: activated by L-rhamnose
note: rhaS
product: positive regulator of the rhaB promoter
translation: MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHGTGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLLPQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKSSLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQRYLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGFLQ
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA085/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA085/annotation6/range/1

https://synbiohub.org/public/SEGA/SEGA085/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA085/1
start: 2818
end: 3654
orientation: reverseComplement

Promoter 3BCD2
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
label: Promoter 3BCD2
note: Promoter 3BCD2
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA085/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA085/annotation12/range/1

https://synbiohub.org/public/SEGA/SEGA085/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA085/1
start: 4742
end: 4770
orientation: inline

https://synbiohub.org/public/SEGA/SEGA081/1
type: ComponentDefinition
persistent identity: SEGA081
display id: SEGA081
version: 1
description: natural linear DNA.
owned by: carbay
owned by: morno
date: 09-SEP-2021
division: UNA
keywords: .
locus: SEGA081
molecule: ds-DNA
organism: unspecified
reference: https://synbiohub.org/user/carbay/SEGA/SEGA081_reference0/1
source: natural DNA sequence
type: DnaRegion
type: SO:0000987
role: SO:0000804
sequence annotation: galK
sequence annotation: GFP
sequence annotation: TcR
sequence annotation: rrnC
sequence annotation: pstS
sequence annotation: L3S2P11 Terminator
sequence annotation: rhaB promoter
sequence annotation: Ubiquitin
sequence annotation: low TIR
sequence annotation: EM7 promoter
sequence annotation: glmS
sequence annotation: Terminator rrnB T1
sequence annotation: L3S3P22 Terminator (synthetic)
sequence annotation: rhaS
sequence annotation: rhaR
sequence annotation: Promoter 3BCD2
sequence: https://synbiohub.org/public/SEGA/SEGA081_seq/1

galK
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
EC number: 2.7.1.6
codon start: 1
db xref: ASAP:ABE-0002568
db xref: EcoGene:EG10363
db xref: GI:16128725
db xref: GeneID:945358
db xref: UniProtKB/Swiss-Prot:P0A6T3
function: enzyme; Degradation of small molecules: Carbon compounds
gene: galK
gene synonym: ECK0746; galA; JW0740
label: galK
locus tag: b0757
note: galK
product: galactokinase
protein id: NP_415278.1
translation: MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVLPCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLDAPIVAHENYQWANYVRGVVKHLQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQEAENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTLVGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTVEAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGGGFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA081/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA081/annotation2/range/1

https://synbiohub.org/public/SEGA/SEGA081/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA081/1
start: 605
end: 1753
orientation: reverseComplement

GFP
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
codon start: 1
label: GFP
note: GFP
product: green fluorescent protein
translation: SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYK
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA081/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA081/annotation10/range/1

https://synbiohub.org/public/SEGA/SEGA081/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA081/1
start: 4257
end: 4967
orientation: inline

TcR
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
codon start: 1
gene: tet
label: TcR
note: TcR
note: confers resistance to tetracycline
product: tetracycline efflux protein
translation: MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHYGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA081/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA081/annotation13/range/1

https://synbiohub.org/public/SEGA/SEGA081/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA081/1
start: 5098
end: 6288
orientation: inline

rrnC
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
label: rrnC
note: rrnC
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA081/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA081/annotation14/range/1

https://synbiohub.org/public/SEGA/SEGA081/annotation14/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA081/1
start: 6299
end: 6339
orientation: inline

pstS
type: SequenceAnnotation
persistent identity: annotation15
display id: annotation15
version: 1
codon start: 1
label: pstS
note: pstS
translation: MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKWADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA081/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA081/annotation15/range/1

https://synbiohub.org/public/SEGA/SEGA081/annotation15/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA081/1
start: 6534
end: 6781
orientation: inline

L3S2P11 Terminator
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
label: L3S2P11 Terminator
note: L3S2P11 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA081/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA081/annotation1/range/1

https://synbiohub.org/public/SEGA/SEGA081/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA081/1
start: 538
end: 594
orientation: inline

rhaB promoter
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
label: rhaB promoter
note: promoter of the E. coli rhaBAD operon, conferring tight induction with L-rhamnose and repression with D-glucose in the presence of RhaR and RhaS (Giacalone et al., 2006)
note: rhaB promoter
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA081/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA081/annotation7/range/1

https://synbiohub.org/public/SEGA/SEGA081/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA081/1
start: 3800
end: 3918
orientation: inline

Ubiquitin
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
codon start: 1
label: Ubiquitin
note: Ubiquitin
translation: MQIFVKTLTGKTITLEVEPGSAHHHHHHHHHHAGSSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQEKSTLELVLELQNETQSATSDA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA081/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA081/annotation9/range/1

https://synbiohub.org/public/SEGA/SEGA081/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA081/1
start: 3948
end: 4247
orientation: inline

low TIR
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
note: TIR
note: low TIR
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA081/1
role: SO:0000139
location: https://synbiohub.org/public/SEGA/SEGA081/annotation8/range/1

https://synbiohub.org/public/SEGA/SEGA081/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA081/1
start: 3930
end: 3956
orientation: inline

EM7 promoter
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
label: EM7 promoter
note: EM7 promoter
note: synthetic bacterial promoter
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA081/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA081/annotation3/range/1

https://synbiohub.org/public/SEGA/SEGA081/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA081/1
start: 1783
end: 1830
orientation: reverseComplement

glmS
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
codon start: 1
label: glmS
note: glmS
translation: VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA081/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA081/annotation0/range/1

https://synbiohub.org/public/SEGA/SEGA081/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA081/1
start: 1
end: 528
orientation: inline

Terminator rrnB T1
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
label: Terminator rrnB T1
note: Terminator rrnB T1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA081/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA081/annotation4/range/1

https://synbiohub.org/public/SEGA/SEGA081/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA081/1
start: 1843
end: 1886
orientation: inline

L3S3P22 Terminator (synthetic)
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
label: L3S3P22 Terminator (synthetic)
note: L3S3P22 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA081/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA081/annotation11/range/1

https://synbiohub.org/public/SEGA/SEGA081/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA081/1
start: 4971
end: 5019
orientation: inline

rhaS
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
codon start: 1
gene: E. coli rhaS
label: rhaS
note: activated by L-rhamnose
note: rhaS
product: positive regulator of the rhaB promoter
translation: MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHGTGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLLPQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKSSLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQRYLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGFLQ
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA081/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA081/annotation6/range/1

https://synbiohub.org/public/SEGA/SEGA081/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA081/1
start: 2818
end: 3654
orientation: reverseComplement

rhaR
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
codon start: 1
gene: E. coli rhaR
label: rhaR
note: needed for rhamnose-dependent activation of the rhaB promoter
note: rhaR
product: transcriptional activator of rhaR and rhaS
translation: MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQGAIPGFNASAGQPHWRLGSMGMAQARQVIGQLEHESSQHVPFANEMAELLFGQLVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRHLNSQKD
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA081/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA081/annotation5/range/1

https://synbiohub.org/public/SEGA/SEGA081/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA081/1
start: 1899
end: 2744
orientation: reverseComplement

Promoter 3BCD2
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
label: Promoter 3BCD2
note: Promoter 3BCD2
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA081/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA081/annotation12/range/1

https://synbiohub.org/public/SEGA/SEGA081/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA081/1
start: 5030
end: 5058
orientation: inline

https://synbiohub.org/public/SEGA/SEGA102/1
type: ComponentDefinition
persistent identity: SEGA102
display id: SEGA102
version: 1
description: natural linear DNA.
owned by: carbay
owned by: morno
date: 09-SEP-2021
division: UNA
keywords: .
locus: SEGA102
molecule: ds-DNA
organism: unspecified
reference: https://synbiohub.org/user/carbay/SEGA/SEGA102_reference0/1
source: natural DNA sequence
type: DnaRegion
type: SO:0000987
role: SO:0000804
sequence annotation: rhaR
sequence annotation: rhaB promoter
sequence annotation: rrnC
sequence annotation: TcR
sequence annotation: pstS
sequence annotation: rhaS
sequence annotation: glmS
sequence annotation: L3S2P11 Terminator
sequence annotation: BCD
sequence annotation: high TIR
sequence: https://synbiohub.org/public/SEGA/SEGA102_seq/1

rhaR
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
codon start: 1
gene: E. coli rhaR
label: rhaR
note: needed for rhamnose-dependent activation of the rhaB promoter
note: rhaR
product: transcriptional activator of rhaR and rhaS
translation: MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQGAIPGFNASAGQPHWRLGSMGMAQARQVIGQLEHESSQHVPFANEMAELLFGQLVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRHLNSQKD
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA102/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA102/annotation2/range/1

https://synbiohub.org/public/SEGA/SEGA102/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA102/1
start: 607
end: 1452
orientation: reverseComplement

rhaB promoter
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
label: rhaB promoter
note: promoter of the E. coli rhaBAD operon, conferring tight induction with L-rhamnose and repression with D-glucose in the presence of RhaR and RhaS (Giacalone et al., 2006)
note: rhaB promoter
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA102/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA102/annotation4/range/1

https://synbiohub.org/public/SEGA/SEGA102/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA102/1
start: 2508
end: 2626
orientation: inline

rrnC
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
label: rrnC
note: rrnC
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA102/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA102/annotation8/range/1

https://synbiohub.org/public/SEGA/SEGA102/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA102/1
start: 3835
end: 3875
orientation: inline

TcR
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
codon start: 1
gene: tet
label: TcR
note: TcR
note: confers resistance to tetracycline
product: tetracycline efflux protein
translation: VLPGLLRDIVHSDSIASHYGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVIFGEDRFRWSATMSGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA102/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA102/annotation7/range/1

https://synbiohub.org/public/SEGA/SEGA102/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA102/1
start: 2712
end: 3824
orientation: inline

pstS
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
codon start: 1
label: pstS
note: pstS
translation: MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKWADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA102/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA102/annotation9/range/1

https://synbiohub.org/public/SEGA/SEGA102/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA102/1
start: 4070
end: 4317
orientation: inline

rhaS
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
codon start: 1
gene: E. coli rhaS
label: rhaS
note: activated by L-rhamnose
note: rhaS
product: positive regulator of the rhaB promoter
translation: MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHGTGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLLPQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKSSLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQRYLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGFLQ
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA102/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA102/annotation3/range/1

https://synbiohub.org/public/SEGA/SEGA102/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA102/1
start: 1526
end: 2362
orientation: reverseComplement

glmS
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
codon start: 1
label: glmS
note: glmS
translation: VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA102/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA102/annotation0/range/1

https://synbiohub.org/public/SEGA/SEGA102/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA102/1
start: 1
end: 528
orientation: inline

L3S2P11 Terminator
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
label: L3S2P11 Terminator
note: L3S2P11 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA102/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA102/annotation1/range/1

https://synbiohub.org/public/SEGA/SEGA102/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA102/1
start: 538
end: 594
orientation: inline

BCD
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
codon start: 1
label: BCD
note: BCD
translation: MKAIFVLKHLNHAKEVF
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA102/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA102/annotation6/range/1

https://synbiohub.org/public/SEGA/SEGA102/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA102/1
start: 2656
end: 2711
orientation: inline

high TIR
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
note: TIR
note: high TIR
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA102/1
role: SO:0000139
location: https://synbiohub.org/public/SEGA/SEGA102/annotation5/range/1

https://synbiohub.org/public/SEGA/SEGA102/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA102/1
start: 2638
end: 2664
orientation: inline

https://synbiohub.org/public/SEGA/SEGA040/1
type: ComponentDefinition
persistent identity: SEGA040
display id: SEGA040
version: 1
description: natural linear DNA.
owned by: carbay
owned by: morno
date: 08-SEP-2021
division: UNA
keywords: .
locus: SEGA040
molecule: ds-DNA
organism: unspecified
reference: https://synbiohub.org/public/SEGA/reference0_1/1
source: natural DNA sequence
type: DnaRegion
type: SO:0000987
role: SO:0000804
sequence annotation: GFP
sequence annotation: L3S2P11 Terminator
sequence annotation: TcR
sequence annotation: BCD
sequence annotation: Promoter 3BCD2
sequence annotation: pstS
sequence annotation: rrnC
sequence annotation: L3S3P22 Terminator (synthetic)
sequence annotation: glmS
sequence annotation: rhaS
sequence annotation: rhaR
sequence annotation: high TIR
sequence annotation: rhaB promoter
sequence: https://synbiohub.org/public/SEGA/SEGA040_seq/1

GFP
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
codon start: 1
label: GFP
note: GFP
product: green fluorescent protein
translation: SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYK
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA040/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA040/annotation7/range/1

https://synbiohub.org/public/SEGA/SEGA040/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA040/1
start: 2712
end: 3422
orientation: inline

L3S2P11 Terminator
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
label: L3S2P11 Terminator
note: L3S2P11 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA040/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA040/annotation1/range/1

https://synbiohub.org/public/SEGA/SEGA040/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA040/1
start: 538
end: 594
orientation: inline

TcR
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
codon start: 1
gene: tet
label: TcR
note: TcR
note: confers resistance to tetracycline
product: tetracycline efflux protein
translation: MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHYGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVIFGEDRFRWSATMSGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA040/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA040/annotation10/range/1

https://synbiohub.org/public/SEGA/SEGA040/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA040/1
start: 3553
end: 4743
orientation: inline

BCD
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
codon start: 1
label: BCD
note: BCD
translation: MKAIFVLKHLNHAKEVF
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA040/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA040/annotation6/range/1

https://synbiohub.org/public/SEGA/SEGA040/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA040/1
start: 2656
end: 2711
orientation: inline

Promoter 3BCD2
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
label: Promoter 3BCD2
note: Promoter 3BCD2
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA040/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA040/annotation9/range/1

https://synbiohub.org/public/SEGA/SEGA040/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA040/1
start: 3485
end: 3513
orientation: inline

pstS
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
codon start: 1
label: pstS
note: pstS
translation: MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKWADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA040/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA040/annotation12/range/1

https://synbiohub.org/public/SEGA/SEGA040/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA040/1
start: 4989
end: 5236
orientation: inline

rrnC
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
label: rrnC
note: rrnC
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA040/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA040/annotation11/range/1

https://synbiohub.org/public/SEGA/SEGA040/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA040/1
start: 4754
end: 4794
orientation: inline

L3S3P22 Terminator (synthetic)
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
label: L3S3P22 Terminator (synthetic)
note: L3S3P22 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA040/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA040/annotation8/range/1

https://synbiohub.org/public/SEGA/SEGA040/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA040/1
start: 3426
end: 3474
orientation: inline

glmS
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
codon start: 1
label: glmS
note: glmS
translation: VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA040/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA040/annotation0/range/1

https://synbiohub.org/public/SEGA/SEGA040/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA040/1
start: 1
end: 528
orientation: inline

rhaS
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
codon start: 1
gene: E. coli rhaS
label: rhaS
note: activated by L-rhamnose
note: rhaS
product: positive regulator of the rhaB promoter
translation: MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHGTGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLLPQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKSSLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQRYLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGFLQ
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA040/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA040/annotation3/range/1

https://synbiohub.org/public/SEGA/SEGA040/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA040/1
start: 1526
end: 2362
orientation: reverseComplement

rhaR
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
codon start: 1
gene: E. coli rhaR
label: rhaR
note: needed for rhamnose-dependent activation of the rhaB promoter
note: rhaR
product: transcriptional activator of rhaR and rhaS
translation: MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQGAIPGFNASAGQPHWRLGSMGMAQARQVIGQLEHESSQHVPFANEMAELLFGQLVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRHLNSQKD
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA040/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA040/annotation2/range/1

https://synbiohub.org/public/SEGA/SEGA040/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA040/1
start: 607
end: 1452
orientation: reverseComplement

high TIR
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
note: TIR
note: high TIR
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA040/1
role: SO:0000139
location: https://synbiohub.org/public/SEGA/SEGA040/annotation5/range/1

https://synbiohub.org/public/SEGA/SEGA040/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA040/1
start: 2638
end: 2664
orientation: inline

rhaB promoter
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
label: rhaB promoter
note: promoter of the E. coli rhaBAD operon, conferring tight induction with L-rhamnose and repression with D-glucose in the presence of RhaR and RhaS (Giacalone et al., 2006)
note: rhaB promoter
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA040/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA040/annotation4/range/1

https://synbiohub.org/public/SEGA/SEGA040/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA040/1
start: 2508
end: 2626
orientation: inline

https://synbiohub.org/public/SEGA/SEGA001/1
type: ComponentDefinition
persistent identity: SEGA001
display id: SEGA001
version: 1
description: natural linear DNA.
owned by: carbay
owned by: morno
date: 09-SEP-2021
division: UNA
keywords: .
locus: SEGA001
molecule: ds-DNA
organism: unspecified
reference: https://synbiohub.org/user/carbay/SEGA/SEGA001_reference0/1
source: natural DNA sequence
type: DnaRegion
type: SO:0000987
role: SO:0000804
sequence annotation: glmS
sequence annotation: TcR
sequence annotation: P-J23100
sequence annotation: rrnC
sequence annotation: RBS
sequence annotation: L3S2P11
sequence annotation: pstS
sequence: https://synbiohub.org/public/SEGA/SEGA001_seq/1

glmS
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
codon start: 1
label: glmS
note: glmS
translation: VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA001/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA001/annotation0/range/1

https://synbiohub.org/public/SEGA/SEGA001/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA001/1
start: 1
end: 528
orientation: inline

TcR
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
codon start: 1
gene: tet
label: TcR
note: TcR
note: confers resistance to tetracycline
product: tetracycline efflux protein
translation: VLPGLLRDIVHSDSIASHYGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVIFGEDRFRWSATMSGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA001/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA001/annotation4/range/1

https://synbiohub.org/public/SEGA/SEGA001/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA001/1
start: 662
end: 1774
orientation: inline

P-J23100
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
label: P-J23100
note: P-J23100
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA001/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA001/annotation2/range/1

https://synbiohub.org/public/SEGA/SEGA001/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA001/1
start: 604
end: 638
orientation: inline

rrnC
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
label: rrnC
note: rrnC
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA001/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA001/annotation5/range/1

https://synbiohub.org/public/SEGA/SEGA001/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA001/1
start: 1785
end: 1825
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
note: RBS
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA001/1
role: SO:0000139
location: https://synbiohub.org/public/SEGA/SEGA001/annotation3/range/1

https://synbiohub.org/public/SEGA/SEGA001/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA001/1
start: 650
end: 661
orientation: inline

L3S2P11
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
label: L3S2P11
note: L3S2P11 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA001/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA001/annotation1/range/1

https://synbiohub.org/public/SEGA/SEGA001/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA001/1
start: 538
end: 594
orientation: inline

pstS
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
codon start: 1
label: pstS
note: pstS
translation: MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKWADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA001/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA001/annotation6/range/1

https://synbiohub.org/public/SEGA/SEGA001/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA001/1
start: 2020
end: 2267
orientation: inline

https://synbiohub.org/public/SEGA/SEGA014/1
type: ComponentDefinition
persistent identity: SEGA014
display id: SEGA014
version: 1
description: natural linear DNA.
owned by: carbay
owned by: morno
date: 09-SEP-2021
division: UNA
keywords: .
locus: SEGA014
molecule: ds-DNA
organism: unspecified
reference: https://synbiohub.org/user/carbay/SEGA/SEGA014_reference0/1
source: natural DNA sequence
type: DnaRegion
type: SO:0000987
role: SO:0000804
sequence annotation: rrnC
sequence annotation: TcR
sequence annotation: Promoter 3BCD2
sequence annotation: PROTi-Linker
sequence annotation: glmS
sequence annotation: P-J23100
sequence annotation: L3S3P22 Terminator (synthetic)
sequence annotation: TEV site
sequence annotation: GFP
sequence annotation: low TIR
sequence annotation: pstS
sequence annotation: L3S2P11 Terminator
sequence: https://synbiohub.org/public/SEGA/SEGA014_seq/1

rrnC
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
label: rrnC
note: rrnC
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA014/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA014/annotation10/range/1

https://synbiohub.org/public/SEGA/SEGA014/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA014/1
start: 2767
end: 2807
orientation: inline

TcR
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
codon start: 1
gene: tet
label: TcR
note: TcR
note: confers resistance to tetracycline
product: tetracycline efflux protein
translation: MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHYGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVIFGEDRFRWSATMSGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA014/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA014/annotation9/range/1

https://synbiohub.org/public/SEGA/SEGA014/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA014/1
start: 1566
end: 2756
orientation: inline

Promoter 3BCD2
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
label: Promoter 3BCD2
note: Promoter 3BCD2
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA014/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA014/annotation8/range/1

https://synbiohub.org/public/SEGA/SEGA014/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA014/1
start: 1498
end: 1526
orientation: inline

PROTi-Linker
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
label: PROTi-Linker
note: PROTi-Linker
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA014/1
role: SO:0001411
location: https://synbiohub.org/public/SEGA/SEGA014/annotation5/range/1

https://synbiohub.org/public/SEGA/SEGA014/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA014/1
start: 689
end: 724
orientation: inline

glmS
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
codon start: 1
label: glmS
note: glmS
translation: VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA014/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA014/annotation0/range/1

https://synbiohub.org/public/SEGA/SEGA014/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA014/1
start: 1
end: 528
orientation: inline

P-J23100
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
label: P-J23100
note: P-J23100
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA014/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA014/annotation2/range/1

https://synbiohub.org/public/SEGA/SEGA014/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA014/1
start: 604
end: 638
orientation: inline

L3S3P22 Terminator (synthetic)
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
label: L3S3P22 Terminator (synthetic)
note: L3S3P22 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA014/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA014/annotation7/range/1

https://synbiohub.org/public/SEGA/SEGA014/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA014/1
start: 1439
end: 1487
orientation: inline

TEV site
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
codon start: 1
label: TEV site
note: TEV site
product: tobacco etch virus (TEV) protease recognition and cleavage site
translation: MENLYFQ
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA014/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA014/annotation4/range/1

https://synbiohub.org/public/SEGA/SEGA014/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA014/1
start: 668
end: 688
orientation: inline

GFP
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
codon start: 1
label: GFP
note: GFP
product: green fluorescent protein
translation: SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYK
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA014/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA014/annotation6/range/1

https://synbiohub.org/public/SEGA/SEGA014/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA014/1
start: 725
end: 1435
orientation: inline

low TIR
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
note: TIR
note: low TIR
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA014/1
role: SO:0000139
location: https://synbiohub.org/public/SEGA/SEGA014/annotation3/range/1

https://synbiohub.org/public/SEGA/SEGA014/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA014/1
start: 650
end: 676
orientation: inline

pstS
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
codon start: 1
label: pstS
note: pstS
translation: MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKWADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA014/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA014/annotation11/range/1

https://synbiohub.org/public/SEGA/SEGA014/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA014/1
start: 3002
end: 3249
orientation: inline

L3S2P11 Terminator
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
label: L3S2P11 Terminator
note: L3S2P11 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA014/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA014/annotation1/range/1

https://synbiohub.org/public/SEGA/SEGA014/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA014/1
start: 538
end: 594
orientation: inline

https://synbiohub.org/public/SEGA/SEGA005/1
type: ComponentDefinition
persistent identity: SEGA005
display id: SEGA005
version: 1
description: natural linear DNA.
owned by: carbay
owned by: morno
date: 09-SEP-2021
division: UNA
keywords: .
locus: SEGA005
molecule: ds-DNA
organism: unspecified
reference: https://synbiohub.org/user/carbay/SEGA/SEGA005_reference0/1
source: natural DNA sequence
type: DnaRegion
type: SO:0000987
role: SO:0000804
sequence annotation: GFP
sequence annotation: TcR
sequence annotation: Promoter 3BCD2
sequence annotation: rrnC
sequence annotation: P-J23100
sequence annotation: BCD
sequence annotation: low TIR
sequence annotation: L3S2P11 Terminator
sequence annotation: pstS
sequence annotation: glmS
sequence annotation: L3S3P22 Terminator (synthetic)
sequence: https://synbiohub.org/public/SEGA/SEGA005_seq/1

GFP
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
codon start: 1
label: GFP
note: GFP
product: green fluoresent protein
translation: SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYK
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA005/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA005/annotation5/range/1

https://synbiohub.org/public/SEGA/SEGA005/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA005/1
start: 724
end: 1434
orientation: inline

TcR
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
codon start: 1
gene: tet
label: TcR
note: TcR
note: confers resistance to tetracycline
product: tetracycline efflux protein
translation: MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHYGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVIFGEDRFRWSATMSGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA005/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA005/annotation8/range/1

https://synbiohub.org/public/SEGA/SEGA005/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA005/1
start: 1565
end: 2755
orientation: inline

Promoter 3BCD2
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
label: Promoter 3BCD2
note: Promoter 3BCD2
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA005/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA005/annotation7/range/1

https://synbiohub.org/public/SEGA/SEGA005/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA005/1
start: 1497
end: 1525
orientation: inline

rrnC
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
label: rrnC
note: rrnC
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA005/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA005/annotation9/range/1

https://synbiohub.org/public/SEGA/SEGA005/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA005/1
start: 2766
end: 2806
orientation: inline

P-J23100
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
label: P-J23100
note: P-J23100
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA005/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA005/annotation2/range/1

https://synbiohub.org/public/SEGA/SEGA005/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA005/1
start: 604
end: 638
orientation: inline

BCD
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
codon start: 1
label: BCD
note: BCD
translation: MKAIFVLKHLNHAKEVF
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA005/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA005/annotation4/range/1

https://synbiohub.org/public/SEGA/SEGA005/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA005/1
start: 668
end: 723
orientation: inline

low TIR
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
note: TIR
note: low TIR
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA005/1
role: SO:0000139
location: https://synbiohub.org/public/SEGA/SEGA005/annotation3/range/1

https://synbiohub.org/public/SEGA/SEGA005/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA005/1
start: 650
end: 676
orientation: inline

L3S2P11 Terminator
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
label: L3S2P11 Terminator
note: L3S2P11 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA005/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA005/annotation1/range/1

https://synbiohub.org/public/SEGA/SEGA005/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA005/1
start: 538
end: 594
orientation: inline

pstS
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
codon start: 1
label: pstS
note: pstS
translation: MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKWADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA005/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA005/annotation10/range/1

https://synbiohub.org/public/SEGA/SEGA005/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA005/1
start: 3001
end: 3248
orientation: inline

glmS
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
codon start: 1
label: glmS
note: glmS
translation: VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA005/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA005/annotation0/range/1

https://synbiohub.org/public/SEGA/SEGA005/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA005/1
start: 1
end: 528
orientation: inline

L3S3P22 Terminator (synthetic)
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
label: L3S3P22 Terminator (synthetic)
note: L3S3P22 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA005/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA005/annotation6/range/1

https://synbiohub.org/public/SEGA/SEGA005/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA005/1
start: 1438
end: 1486
orientation: inline

https://synbiohub.org/public/SEGA/SEGA011/1
type: ComponentDefinition
persistent identity: SEGA011
display id: SEGA011
version: 1
description: natural linear DNA.
owned by: carbay
owned by: morno
date: 09-SEP-2021
division: UNA
keywords: .
locus: SEGA011
molecule: ds-DNA
organism: unspecified
reference: https://synbiohub.org/user/carbay/SEGA/SEGA011_reference0/1
source: natural DNA sequence
type: DnaRegion
type: SO:0000987
role: SO:0000804
sequence annotation: TEV site
sequence annotation: pstS
sequence annotation: glmS
sequence annotation: L3S3P22 Terminator (synthetic)
sequence annotation: low TIR
sequence annotation: L3S2P11 Terminator
sequence annotation: GFP
sequence annotation: TcR
sequence annotation: rrnC
sequence annotation: P-J23100
sequence annotation: Promoter 3BCD2
sequence: https://synbiohub.org/public/SEGA/SEGA011_seq/1

TEV site
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
codon start: 1
label: TEV site
note: TEV site
product: tobacco etch virus (TEV) protease recognition and cleavage site
translation: MENLYFQ
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA011/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA011/annotation4/range/1

https://synbiohub.org/public/SEGA/SEGA011/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA011/1
start: 668
end: 688
orientation: inline

pstS
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
codon start: 1
label: pstS
note: pstS
translation: MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKWADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA011/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA011/annotation10/range/1

https://synbiohub.org/public/SEGA/SEGA011/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA011/1
start: 2966
end: 3213
orientation: inline

glmS
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
codon start: 1
label: glmS
note: glmS
translation: VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA011/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA011/annotation0/range/1

https://synbiohub.org/public/SEGA/SEGA011/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA011/1
start: 1
end: 528
orientation: inline

L3S3P22 Terminator (synthetic)
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
label: L3S3P22 Terminator (synthetic)
note: L3S3P22 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA011/1
role: SO:0000139
location: https://synbiohub.org/public/SEGA/SEGA011/annotation6/range/1

https://synbiohub.org/public/SEGA/SEGA011/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA011/1
start: 1403
end: 1451
orientation: inline

low TIR
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
note: TIR
note: low TIR
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA011/1
role: SO:0000139
location: https://synbiohub.org/public/SEGA/SEGA011/annotation3/range/1

https://synbiohub.org/public/SEGA/SEGA011/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA011/1
start: 650
end: 676
orientation: inline

L3S2P11 Terminator
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
label: L3S2P11 Terminator
note: L3S2P11 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA011/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA011/annotation1/range/1

https://synbiohub.org/public/SEGA/SEGA011/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA011/1
start: 538
end: 594
orientation: inline

GFP
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
codon start: 1
label: GFP
note: GFP
product: green fluorescent protein
translation: SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYK
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA011/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA011/annotation5/range/1

https://synbiohub.org/public/SEGA/SEGA011/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA011/1
start: 689
end: 1399
orientation: inline

TcR
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
codon start: 1
gene: tet
label: TcR
note: TcR
note: confers resistance to tetracycline
product: tetracycline efflux protein
translation: MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHYGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVIFGEDRFRWSATMSGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA011/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA011/annotation8/range/1

https://synbiohub.org/public/SEGA/SEGA011/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA011/1
start: 1530
end: 2720
orientation: inline

rrnC
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
label: rrnC
note: rrnC
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA011/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA011/annotation9/range/1

https://synbiohub.org/public/SEGA/SEGA011/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA011/1
start: 2731
end: 2771
orientation: inline

P-J23100
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
label: P-J23100
note: P-J23100
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA011/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA011/annotation2/range/1

https://synbiohub.org/public/SEGA/SEGA011/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA011/1
start: 604
end: 638
orientation: inline

Promoter 3BCD2
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
label: Promoter 3BCD2
note: Promoter 3BCD2
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA011/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA011/annotation7/range/1

https://synbiohub.org/public/SEGA/SEGA011/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA011/1
start: 1462
end: 1490
orientation: inline

https://synbiohub.org/public/SEGA/SEGA057/1
type: ComponentDefinition
persistent identity: SEGA057
display id: SEGA057
version: 1
description: natural linear DNA.
owned by: carbay
owned by: morno
date: 08-SEP-2021
division: UNA
keywords: .
locus: SEGA057
molecule: ds-DNA
organism: unspecified
reference: https://synbiohub.org/user/carbay/SEGA/SEGA057_reference0/1
source: natural DNA sequence
type: DnaRegion
type: SO:0000987
role: SO:0000804
sequence annotation: rrnC
sequence annotation: lac operator
sequence annotation: glmS
sequence annotation: medium TIR
sequence annotation: L3S3P22 Terminator (synthetic)
sequence annotation: lacI
sequence annotation: BCD
sequence annotation: lacI promoter
sequence annotation: L3S2P11 Terminator
sequence annotation: Promoter 3BCD2
sequence annotation: TcR
sequence annotation: pstS
sequence annotation: T7 promoter
sequence annotation: GFP
sequence: https://synbiohub.org/public/SEGA/SEGA057_seq/1

rrnC
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
label: rrnC
note: rrnC
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA057/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA057/annotation12/range/1

https://synbiohub.org/public/SEGA/SEGA057/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA057/1
start: 3966
end: 4006
orientation: inline

lac operator
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
bound moiety: lac repressor encoded by lacI
feature type: protein_bind
label: lac operator
note: The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG).
note: lac operator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA057/1
role: SO:0000110
location: https://synbiohub.org/public/SEGA/SEGA057/annotation5/range/1

https://synbiohub.org/public/SEGA/SEGA057/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA057/1
start: 1814
end: 1838
orientation: inline

glmS
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
codon start: 1
label: glmS
note: glmS
translation: VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA057/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA057/annotation0/range/1

https://synbiohub.org/public/SEGA/SEGA057/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA057/1
start: 1
end: 528
orientation: inline

medium TIR
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
note: TIR
note: medium TIR
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA057/1
role: SO:0000139
location: https://synbiohub.org/public/SEGA/SEGA057/annotation6/range/1

https://synbiohub.org/public/SEGA/SEGA057/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA057/1
start: 1850
end: 1876
orientation: inline

L3S3P22 Terminator (synthetic)
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
label: L3S3P22 Terminator (synthetic)
note: L3S3P22 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA057/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA057/annotation9/range/1

https://synbiohub.org/public/SEGA/SEGA057/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA057/1
start: 2638
end: 2686
orientation: inline

lacI
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
codon start: 1
gene: lacI
label: lacI
note: The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG).
note: lacI
product: lac repressor
translation: MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA057/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA057/annotation2/range/1

https://synbiohub.org/public/SEGA/SEGA057/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA057/1
start: 604
end: 1686
orientation: reverseComplement

BCD
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
codon start: 1
label: BCD
note: BCD
translation: MKAIFVLKHLNHAKEVF
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA057/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA057/annotation7/range/1

https://synbiohub.org/public/SEGA/SEGA057/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA057/1
start: 1868
end: 1923
orientation: inline

lacI promoter
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
gene: lacI
label: lacI promoter
note: lacI promoter
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA057/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA057/annotation3/range/1

https://synbiohub.org/public/SEGA/SEGA057/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA057/1
start: 1687
end: 1764
orientation: reverseComplement

L3S2P11 Terminator
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
label: L3S2P11 Terminator
note: L3S2P11 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA057/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA057/annotation1/range/1

https://synbiohub.org/public/SEGA/SEGA057/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA057/1
start: 538
end: 594
orientation: inline

Promoter 3BCD2
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
label: Promoter 3BCD2
note: Promoter 3BCD2
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA057/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA057/annotation10/range/1

https://synbiohub.org/public/SEGA/SEGA057/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA057/1
start: 2697
end: 2725
orientation: inline

TcR
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
codon start: 1
gene: tet
label: TcR
note: TcR
note: confers resistance to tetracycline
product: tetracycline efflux protein
translation: MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHYGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVIFGEDRFRWSATMSGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA057/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA057/annotation11/range/1

https://synbiohub.org/public/SEGA/SEGA057/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA057/1
start: 2765
end: 3955
orientation: inline

pstS
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
codon start: 1
label: pstS
note: pstS
translation: MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKWADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA057/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA057/annotation13/range/1

https://synbiohub.org/public/SEGA/SEGA057/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA057/1
start: 4201
end: 4448
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
label: T7 promoter
note: T7 promoter
note: promoter for bacteriophage T7 RNA polymerase
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA057/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA057/annotation4/range/1

https://synbiohub.org/public/SEGA/SEGA057/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA057/1
start: 1795
end: 1813
orientation: inline

GFP
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
codon start: 1
label: GFP
note: GFP
product: green fluorescent protein
translation: SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYK
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA057/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA057/annotation8/range/1

https://synbiohub.org/public/SEGA/SEGA057/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA057/1
start: 1924
end: 2634
orientation: inline

https://synbiohub.org/public/SEGA/SEGA088/1
type: ComponentDefinition
persistent identity: SEGA088
display id: SEGA088
version: 1
description: natural linear DNA.
modified: 2021-09-20T12:43:02
owned by: carbay
owned by: morno
date: 09-SEP-2021
division: UNA
keywords: .
locus: SEGA088
molecule: ds-DNA
organism: unspecified
reference: https://synbiohub.org/user/carbay/SEGA/SEGA088_reference0/1
source: natural DNA sequence
type: DnaRegion
type: SO:0000987
role: SO:0000804
sequence annotation: EM7 promoter
sequence annotation: PROTi-Linker
sequence annotation: rrnC
sequence annotation: pstS
sequence annotation: L3S2P11 Terminator
sequence annotation: L3S3P22 Terminator (synthetic)
sequence annotation: glmS
sequence annotation: medium TIR
sequence annotation: rhaB promoter
sequence annotation: TEV site
sequence annotation: TcR
sequence annotation: Promoter 3BCD2
sequence annotation: GFP
sequence annotation: galK
sequence annotation: Terminator rrnB T1
sequence annotation: rhaS
sequence annotation: rhaR
sequence: https://synbiohub.org/public/SEGA/SEGA088_seq/1

EM7 promoter
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
label: EM7 promoter
note: EM7 promoter
note: synthetic bacterial promoter
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA088/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA088/annotation3/range/1

https://synbiohub.org/public/SEGA/SEGA088/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA088/1
start: 1783
end: 1830
orientation: reverseComplement

PROTi-Linker
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
label: PROTi-Linker
note: PROTi-Linker
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA088/1
role: SO:0001411
location: https://synbiohub.org/public/SEGA/SEGA088/annotation10/range/1

https://synbiohub.org/public/SEGA/SEGA088/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA088/1
start: 3969
end: 4004
orientation: inline

rrnC
type: SequenceAnnotation
persistent identity: annotation15
display id: annotation15
version: 1
label: rrnC
note: rrnC
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA088/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA088/annotation15/range/1

https://synbiohub.org/public/SEGA/SEGA088/annotation15/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA088/1
start: 6047
end: 6087
orientation: inline

pstS
type: SequenceAnnotation
persistent identity: annotation16
display id: annotation16
version: 1
codon start: 1
label: pstS
note: pstS
translation: MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKWADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA088/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA088/annotation16/range/1

https://synbiohub.org/public/SEGA/SEGA088/annotation16/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA088/1
start: 6282
end: 6529
orientation: inline

L3S2P11 Terminator
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
label: L3S2P11 Terminator
note: L3S2P11 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA088/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA088/annotation1/range/1

https://synbiohub.org/public/SEGA/SEGA088/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA088/1
start: 538
end: 594
orientation: inline

L3S3P22 Terminator (synthetic)
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
label: L3S3P22 Terminator (synthetic)
note: L3S3P22 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA088/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA088/annotation12/range/1

https://synbiohub.org/public/SEGA/SEGA088/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA088/1
start: 4719
end: 4767
orientation: inline

glmS
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
codon start: 1
label: glmS
note: glmS
translation: VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA088/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA088/annotation0/range/1

https://synbiohub.org/public/SEGA/SEGA088/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA088/1
start: 1
end: 528
orientation: inline

medium TIR
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
note: TIR
note: medium TIR
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA088/1
role: SO:0000139
location: https://synbiohub.org/public/SEGA/SEGA088/annotation8/range/1

https://synbiohub.org/public/SEGA/SEGA088/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA088/1
start: 3930
end: 3956
orientation: inline

rhaB promoter
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
label: rhaB promoter
note: promoter of the E. coli rhaBAD operon, conferring tight induction with L-rhamnose and repression with D-glucose in the presence of RhaR and RhaS (Giacalone et al., 2006)
note: rhaB promoter
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA088/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA088/annotation7/range/1

https://synbiohub.org/public/SEGA/SEGA088/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA088/1
start: 3800
end: 3918
orientation: inline

TEV site
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
codon start: 1
label: TEV site
note: TEV site
product: tobacco etch virus (TEV) protease recognition and cleavage site
translation: MENLYFQ
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA088/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA088/annotation9/range/1

https://synbiohub.org/public/SEGA/SEGA088/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA088/1
start: 3948
end: 3968
orientation: inline

TcR
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
codon start: 1
gene: tet
label: TcR
note: TcR
note: confers resistance to tetracycline
product: tetracycline efflux protein
translation: MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHYGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA088/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA088/annotation14/range/1

https://synbiohub.org/public/SEGA/SEGA088/annotation14/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA088/1
start: 4846
end: 6036
orientation: inline

Promoter 3BCD2
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
label: Promoter 3BCD2
note: Promoter 3BCD2
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA088/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA088/annotation13/range/1

https://synbiohub.org/public/SEGA/SEGA088/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA088/1
start: 4778
end: 4806
orientation: inline

GFP
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
codon start: 1
label: GFP
note: GFP
product: green fluorescent protein
translation: SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYK
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA088/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA088/annotation11/range/1

https://synbiohub.org/public/SEGA/SEGA088/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA088/1
start: 4005
end: 4715
orientation: inline

galK
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
EC number: 2.7.1.6
codon start: 1
db xref: ASAP:ABE-0002568
db xref: EcoGene:EG10363
db xref: GI:16128725
db xref: GeneID:945358
db xref: UniProtKB/Swiss-Prot:P0A6T3
function: enzyme; Degradation of small molecules: Carbon compounds
gene: galK
gene synonym: ECK0746; galA; JW0740
label: galK
locus tag: b0757
note: galK
product: galactokinase
protein id: NP_415278.1
translation: MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVLPCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLDAPIVAHENYQWANYVRGVVKHLQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQEAENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTLVGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTVEAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGGGFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA088/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA088/annotation2/range/1

https://synbiohub.org/public/SEGA/SEGA088/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA088/1
start: 605
end: 1753
orientation: reverseComplement

Terminator rrnB T1
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
label: Terminator rrnB T1
note: Terminator rrnB T1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA088/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA088/annotation4/range/1

https://synbiohub.org/public/SEGA/SEGA088/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA088/1
start: 1843
end: 1886
orientation: inline

rhaS
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
codon start: 1
gene: E. coli rhaS
label: rhaS
note: activated by L-rhamnose
note: rhaS
product: positive regulator of the rhaB promoter
translation: MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHGTGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLLPQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKSSLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQRYLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGFLQ
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA088/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA088/annotation6/range/1

https://synbiohub.org/public/SEGA/SEGA088/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA088/1
start: 2818
end: 3654
orientation: reverseComplement

rhaR
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
codon start: 1
gene: E. coli rhaR
label: rhaR
note: needed for rhamnose-dependent activation of the rhaB promoter
note: rhaR
product: transcriptional activator of rhaR and rhaS
translation: MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQGAIPGFNASAGQPHWRLGSMGMAQARQVIGQLEHESSQHVPFANEMAELLFGQLVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRHLNSQKD
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA088/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA088/annotation5/range/1

https://synbiohub.org/public/SEGA/SEGA088/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA088/1
start: 1899
end: 2744
orientation: reverseComplement

https://synbiohub.org/public/SEGA/SEGA025/1
type: ComponentDefinition
persistent identity: SEGA025
display id: SEGA025
version: 1
description: natural linear DNA.
owned by: carbay
owned by: morno
date: 08-SEP-2021
division: UNA
keywords: .
locus: SEGA025
molecule: ds-DNA
organism: unspecified
reference: https://synbiohub.org/public/SEGA/reference0_1/1
source: natural DNA sequence
type: DnaRegion
type: SO:0000987
role: SO:0000804
sequence annotation: trc promoter
sequence annotation: GFP
sequence annotation: glmS
sequence annotation: lacI
sequence annotation: lacO
sequence annotation: pstS
sequence annotation: L3S2P11 Terminator
sequence annotation: Ubiquitin
sequence annotation: Promoter 3BCD2
sequence annotation: L3S3P22 Terminator (synthetic)
sequence annotation: rrnC
sequence annotation: TcR
sequence annotation: high TIR
sequence annotation: lacI promoter
sequence: https://synbiohub.org/public/SEGA/SEGA025_seq/1

trc promoter
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
label: trc promoter
note: strong E. coli promoter; hybrid between the trp and lac UV5 promoters
note: trc promoter
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA025/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA025/annotation4/range/1

https://synbiohub.org/public/SEGA/SEGA025/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA025/1
start: 1795
end: 1824
orientation: inline

GFP
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
codon start: 1
label: GFP
note: GFP
product: green fluorescent protein
translation: SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYK
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA025/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA025/annotation8/range/1

https://synbiohub.org/public/SEGA/SEGA025/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA025/1
start: 2187
end: 2897
orientation: inline

glmS
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
codon start: 1
label: glmS
note: glmS
translation: VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA025/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA025/annotation0/range/1

https://synbiohub.org/public/SEGA/SEGA025/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA025/1
start: 1
end: 528
orientation: inline

lacI
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
codon start: 1
gene: lacI
label: lacI
note: The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG).
note: lacI
product: lac repressor
translation: MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA025/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA025/annotation2/range/1

https://synbiohub.org/public/SEGA/SEGA025/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA025/1
start: 604
end: 1686
orientation: reverseComplement

lacO
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
bound moiety: lac repressor
feature type: protein_bind
label: lacO
note: lac operator
note: lacO
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA025/1
role: SO:0000110
location: https://synbiohub.org/public/SEGA/SEGA025/annotation5/range/1

https://synbiohub.org/public/SEGA/SEGA025/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA025/1
start: 1825
end: 1848
orientation: inline

pstS
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
codon start: 1
label: pstS
note: pstS
translation: MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKWADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA025/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA025/annotation13/range/1

https://synbiohub.org/public/SEGA/SEGA025/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA025/1
start: 4464
end: 4711
orientation: inline

L3S2P11 Terminator
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
label: L3S2P11 Terminator
note: L3S2P11 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA025/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA025/annotation1/range/1

https://synbiohub.org/public/SEGA/SEGA025/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA025/1
start: 538
end: 594
orientation: inline

Ubiquitin
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
codon start: 1
label: Ubiquitin
note: Ubiquitin
translation: MQIFVKTLTGKTITLEVEPGSAHHHHHHHHHHAGSSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQEKSTLELVLELQNETQSATSDA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA025/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA025/annotation7/range/1

https://synbiohub.org/public/SEGA/SEGA025/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA025/1
start: 1878
end: 2177
orientation: inline

Promoter 3BCD2
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
label: Promoter 3BCD2
note: Promoter 3BCD2
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA025/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA025/annotation10/range/1

https://synbiohub.org/public/SEGA/SEGA025/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA025/1
start: 2960
end: 2988
orientation: inline

L3S3P22 Terminator (synthetic)
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
label: L3S3P22 Terminator (synthetic)
note: L3S3P22 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA025/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA025/annotation9/range/1

https://synbiohub.org/public/SEGA/SEGA025/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA025/1
start: 2901
end: 2949
orientation: inline

rrnC
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
label: rrnC
note: rrnC
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA025/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA025/annotation12/range/1

https://synbiohub.org/public/SEGA/SEGA025/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA025/1
start: 4229
end: 4269
orientation: inline

TcR
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
codon start: 1
gene: tet
label: TcR
note: TcR
note: confers resistance to tetracycline
product: tetracycline efflux protein
translation: MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHYGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVIFGEDRFRWSATMSGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA025/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA025/annotation11/range/1

https://synbiohub.org/public/SEGA/SEGA025/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA025/1
start: 3028
end: 4218
orientation: inline

high TIR
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
note: TIR
note: high TIR
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA025/1
role: SO:0000139
location: https://synbiohub.org/public/SEGA/SEGA025/annotation6/range/1

https://synbiohub.org/public/SEGA/SEGA025/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA025/1
start: 1860
end: 1886
orientation: inline

lacI promoter
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
gene: lacI
label: lacI promoter
note: lacI promoter
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA025/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA025/annotation3/range/1

https://synbiohub.org/public/SEGA/SEGA025/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA025/1
start: 1687
end: 1764
orientation: reverseComplement

https://synbiohub.org/public/SEGA/SEGA013/1
type: ComponentDefinition
persistent identity: SEGA013
display id: SEGA013
version: 1
description: natural linear DNA.
owned by: carbay
owned by: morno
date: 09-SEP-2021
division: UNA
keywords: .
locus: SEGA013
molecule: ds-DNA
organism: unspecified
reference: https://synbiohub.org/user/carbay/SEGA/SEGA013_reference0/1
source: natural DNA sequence
type: DnaRegion
type: SO:0000987
role: SO:0000804
sequence annotation: L3S2P11 Terminator
sequence annotation: L3S3P22 Terminator (synthetic)
sequence annotation: TcR
sequence annotation: rrnC
sequence annotation: high TIR
sequence annotation: TEV site
sequence annotation: Promoter 3BCD2
sequence annotation: GFP
sequence annotation: pstS
sequence annotation: P-J23100
sequence annotation: glmS
sequence: https://synbiohub.org/public/SEGA/SEGA013_seq/1

L3S2P11 Terminator
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
label: L3S2P11 Terminator
note: L3S2P11 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA013/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA013/annotation1/range/1

https://synbiohub.org/public/SEGA/SEGA013/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA013/1
start: 538
end: 594
orientation: inline

L3S3P22 Terminator (synthetic)
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
label: L3S3P22 Terminator (synthetic)
note: L3S3P22 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA013/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA013/annotation6/range/1

https://synbiohub.org/public/SEGA/SEGA013/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA013/1
start: 1403
end: 1451
orientation: inline

TcR
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
codon start: 1
gene: tet
label: TcR
note: TcR
note: confers resistance to tetracycline
product: tetracycline efflux protein
translation: MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHYGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVIFGEDRFRWSATMSGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA013/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA013/annotation8/range/1

https://synbiohub.org/public/SEGA/SEGA013/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA013/1
start: 1530
end: 2720
orientation: inline

rrnC
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
label: rrnC
note: rrnC
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA013/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA013/annotation9/range/1

https://synbiohub.org/public/SEGA/SEGA013/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA013/1
start: 2731
end: 2771
orientation: inline

high TIR
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
note: TIR
note: high TIR
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA013/1
role: SO:0000139
location: https://synbiohub.org/public/SEGA/SEGA013/annotation3/range/1

https://synbiohub.org/public/SEGA/SEGA013/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA013/1
start: 650
end: 676
orientation: inline

TEV site
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
codon start: 1
label: TEV site
note: TEV site
product: tobacco etch virus (TEV) protease recognition and cleavage site
translation: MENLYFQ
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA013/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA013/annotation4/range/1

https://synbiohub.org/public/SEGA/SEGA013/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA013/1
start: 668
end: 688
orientation: inline

Promoter 3BCD2
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
label: Promoter 3BCD2
note: Promoter 3BCD2
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA013/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA013/annotation7/range/1

https://synbiohub.org/public/SEGA/SEGA013/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA013/1
start: 1462
end: 1490
orientation: inline

GFP
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
codon start: 1
label: GFP
note: GFP
product: green fluorescent protein
translation: SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSTLSKDPNEKRDHMVLLEFVTAAGITHGMDELYK
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA013/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA013/annotation5/range/1

https://synbiohub.org/public/SEGA/SEGA013/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA013/1
start: 689
end: 1399
orientation: inline

pstS
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
codon start: 1
label: pstS
note: pstS
translation: MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKWADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA013/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA013/annotation10/range/1

https://synbiohub.org/public/SEGA/SEGA013/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA013/1
start: 2966
end: 3213
orientation: inline

P-J23100
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
label: P-J23100
note: P-J23100
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA013/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA013/annotation2/range/1

https://synbiohub.org/public/SEGA/SEGA013/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA013/1
start: 604
end: 638
orientation: inline

glmS
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
codon start: 1
label: glmS
note: glmS
translation: VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA013/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA013/annotation0/range/1

https://synbiohub.org/public/SEGA/SEGA013/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA013/1
start: 1
end: 528
orientation: inline

https://synbiohub.org/public/SEGA/SEGA043/1
type: ComponentDefinition
persistent identity: SEGA043
display id: SEGA043
version: 1
description: natural linear DNA.
owned by: carbay
owned by: morno
date: 08-SEP-2021
division: UNA
keywords: .
locus: SEGA043
molecule: ds-DNA
organism: unspecified
reference: https://synbiohub.org/user/carbay/SEGA/SEGA043_reference0/1
source: natural DNA sequence
type: DnaRegion
type: SO:0000987
role: SO:0000804
sequence annotation: high TIR
sequence annotation: rrnC
sequence annotation: rhaB promoter
sequence annotation: TcR
sequence annotation: L3S2P11 Terminator
sequence annotation: Ubiquitin
sequence annotation: GFP
sequence annotation: glmS
sequence annotation: rhaS
sequence annotation: Promoter 3BCD2
sequence annotation: L3S3P22 Terminator (synthetic)
sequence annotation: pstS
sequence annotation: rhaR
sequence: https://synbiohub.org/public/SEGA/SEGA043_seq/1

high TIR
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
note: TIR
note: high TIR
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA043/1
role: SO:0000139
location: https://synbiohub.org/public/SEGA/SEGA043/annotation5/range/1

https://synbiohub.org/public/SEGA/SEGA043/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA043/1
start: 2638
end: 2664
orientation: inline

rrnC
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
label: rrnC
note: rrnC
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA043/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA043/annotation11/range/1

https://synbiohub.org/public/SEGA/SEGA043/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA043/1
start: 5007
end: 5047
orientation: inline

rhaB promoter
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
label: rhaB promoter
note: promoter of the E. coli rhaBAD operon, conferring tight induction with L-rhamnose and repression with D-glucose in the presence of RhaR and RhaS (Giacalone et al., 2006)
note: rhaB promoter
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA043/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA043/annotation4/range/1

https://synbiohub.org/public/SEGA/SEGA043/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA043/1
start: 2508
end: 2626
orientation: inline

TcR
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
codon start: 1
gene: tet
label: TcR
note: TcR
note: confers resistance to tetracycline
product: tetracycline efflux protein
translation: MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHYGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVIFGEDRFRWSATMSGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA043/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA043/annotation10/range/1

https://synbiohub.org/public/SEGA/SEGA043/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA043/1
start: 3806
end: 4996
orientation: inline

L3S2P11 Terminator
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
label: L3S2P11 Terminator
note: L3S2P11 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA043/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA043/annotation1/range/1

https://synbiohub.org/public/SEGA/SEGA043/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA043/1
start: 538
end: 594
orientation: inline

Ubiquitin
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
codon start: 1
label: Ubiquitin
note: Ubiquitin
translation: MQIFVKTLTGKTITLEVEPGSAHHHHHHHHHHAGSSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQEKSTLELVLELQNETQSATSDA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA043/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA043/annotation6/range/1

https://synbiohub.org/public/SEGA/SEGA043/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA043/1
start: 2656
end: 2955
orientation: inline

GFP
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
codon start: 1
label: GFP
note: GFP
product: green fluorescent protein
translation: SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYK
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA043/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA043/annotation7/range/1

https://synbiohub.org/public/SEGA/SEGA043/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA043/1
start: 2965
end: 3675
orientation: inline

glmS
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
codon start: 1
label: glmS
note: glmS
translation: VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA043/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA043/annotation0/range/1

https://synbiohub.org/public/SEGA/SEGA043/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA043/1
start: 1
end: 528
orientation: inline

rhaS
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
codon start: 1
gene: E. coli rhaS
label: rhaS
note: activated by L-rhamnose
note: rhaS
product: positive regulator of the rhaB promoter
translation: MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHGTGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLLPQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKSSLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQRYLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGFLQ
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA043/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA043/annotation3/range/1

https://synbiohub.org/public/SEGA/SEGA043/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA043/1
start: 1526
end: 2362
orientation: reverseComplement

Promoter 3BCD2
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
label: Promoter 3BCD2
note: Promoter 3BCD2
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA043/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA043/annotation9/range/1

https://synbiohub.org/public/SEGA/SEGA043/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA043/1
start: 3738
end: 3766
orientation: inline

L3S3P22 Terminator (synthetic)
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
label: L3S3P22 Terminator (synthetic)
note: L3S3P22 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA043/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA043/annotation8/range/1

https://synbiohub.org/public/SEGA/SEGA043/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA043/1
start: 3679
end: 3727
orientation: inline

pstS
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
codon start: 1
label: pstS
note: pstS
translation: MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKWADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA043/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA043/annotation12/range/1

https://synbiohub.org/public/SEGA/SEGA043/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA043/1
start: 5242
end: 5489
orientation: inline

rhaR
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
codon start: 1
gene: E. coli rhaR
label: rhaR
note: needed for rhamnose-dependent activation of the rhaB promoter
note: rhaR
product: transcriptional activator of rhaR and rhaS
translation: MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQGAIPGFNASAGQPHWRLGSMGMAQARQVIGQLEHESSQHVPFANEMAELLFGQLVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRHLNSQKD
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA043/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA043/annotation2/range/1

https://synbiohub.org/public/SEGA/SEGA043/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA043/1
start: 607
end: 1452
orientation: reverseComplement

https://synbiohub.org/public/SEGA/SEGA094/1
type: ComponentDefinition
persistent identity: SEGA094
display id: SEGA094
version: 1
description: natural linear DNA.
owned by: carbay
owned by: morno
date: 09-SEP-2021
division: UNA
keywords: .
locus: SEGA094
molecule: ds-DNA
organism: unspecified
reference: https://synbiohub.org/user/carbay/SEGA/SEGA094_reference0/1
source: natural DNA sequence
type: DnaRegion
type: SO:0000987
role: SO:0000804
sequence annotation: EM7 promoter
sequence annotation: YebFss
sequence annotation: Terminator rrnB T1
sequence annotation: galK
sequence annotation: rhaB promoter
sequence annotation: rrnC
sequence annotation: sfGFP
sequence annotation: L3S2P11 Terminator
sequence annotation: glmS
sequence annotation: rhaS
sequence annotation: Promoter 3BCD2
sequence annotation: rhaR
sequence annotation: pstS
sequence annotation: L3S3P22 Terminator (synthetic)
sequence annotation: medium TIR
sequence annotation: YebF
sequence annotation: TcR
sequence: https://synbiohub.org/public/SEGA/SEGA094_seq/1

EM7 promoter
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
label: EM7 promoter
note: EM7 promoter
note: synthetic bacterial promoter
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA094/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA094/annotation3/range/1

https://synbiohub.org/public/SEGA/SEGA094/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA094/1
start: 1783
end: 1830
orientation: reverseComplement

YebFss
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
codon start: 1
db xref: ASAP:ABE-0006141
db xref: EcoGene:EG11807
db xref: GI:145698278
db xref: GeneID:946363
db xref: UniProtKB/Swiss-Prot:P33219
gene: yebF
gene synonym: ECK1848; JW1836
label: YebFss
locus tag: b1847
note: YebFss
product: yebF signal sequence
protein id: NP_416361.2
translation: MKKRGAFLGLLLVSACASVFA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA094/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA094/annotation9/range/1

https://synbiohub.org/public/SEGA/SEGA094/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA094/1
start: 3948
end: 4010
orientation: inline

Terminator rrnB T1
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
label: Terminator rrnB T1
note: Terminator rrnB T1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA094/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA094/annotation4/range/1

https://synbiohub.org/public/SEGA/SEGA094/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA094/1
start: 1843
end: 1886
orientation: inline

galK
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
EC number: 2.7.1.6
codon start: 1
db xref: ASAP:ABE-0002568
db xref: EcoGene:EG10363
db xref: GI:16128725
db xref: GeneID:945358
db xref: UniProtKB/Swiss-Prot:P0A6T3
function: enzyme; Degradation of small molecules: Carbon compounds
gene: galK
gene synonym: ECK0746; galA; JW0740
label: galK
locus tag: b0757
note: galK
product: galactokinase
protein id: NP_415278.1
translation: MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVLPCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLDAPIVAHENYQWANYVRGVVKHLQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQEAENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTLVGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTVEAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGGGFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA094/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA094/annotation2/range/1

https://synbiohub.org/public/SEGA/SEGA094/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA094/1
start: 605
end: 1753
orientation: reverseComplement

rhaB promoter
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
label: rhaB promoter
note: promoter of the E. coli rhaBAD operon, conferring tight induction with L-rhamnose and repression with D-glucose in the presence of RhaR and RhaS (Giacalone et al., 2006)
note: rhaB promoter
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA094/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA094/annotation7/range/1

https://synbiohub.org/public/SEGA/SEGA094/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA094/1
start: 3800
end: 3918
orientation: inline

rrnC
type: SequenceAnnotation
persistent identity: annotation15
display id: annotation15
version: 1
label: rrnC
note: rrnC
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA094/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA094/annotation15/range/1

https://synbiohub.org/public/SEGA/SEGA094/annotation15/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA094/1
start: 6344
end: 6384
orientation: inline

sfGFP
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
codon start: 1
label: sfGFP
note: sfGFP
product: superfolder GFP
translation: RKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDGTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEFVTAAGITHGMDELYK
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA094/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA094/annotation11/range/1

https://synbiohub.org/public/SEGA/SEGA094/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA094/1
start: 4302
end: 5012
orientation: inline

L3S2P11 Terminator
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
label: L3S2P11 Terminator
note: L3S2P11 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA094/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA094/annotation1/range/1

https://synbiohub.org/public/SEGA/SEGA094/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA094/1
start: 538
end: 594
orientation: inline

glmS
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
codon start: 1
label: glmS
note: glmS
translation: VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA094/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA094/annotation0/range/1

https://synbiohub.org/public/SEGA/SEGA094/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA094/1
start: 1
end: 528
orientation: inline

rhaS
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
codon start: 1
gene: E. coli rhaS
label: rhaS
note: activated by L-rhamnose
note: rhaS
product: positive regulator of the rhaB promoter
translation: MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHGTGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLLPQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKSSLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQRYLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGFLQ
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA094/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA094/annotation6/range/1

https://synbiohub.org/public/SEGA/SEGA094/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA094/1
start: 2818
end: 3654
orientation: reverseComplement

Promoter 3BCD2
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
label: Promoter 3BCD2
note: Promoter 3BCD2
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA094/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA094/annotation13/range/1

https://synbiohub.org/public/SEGA/SEGA094/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA094/1
start: 5075
end: 5103
orientation: inline

rhaR
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
codon start: 1
gene: E. coli rhaR
label: rhaR
note: needed for rhamnose-dependent activation of the rhaB promoter
note: rhaR
product: transcriptional activator of rhaR and rhaS
translation: MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQGAIPGFNASAGQPHWRLGSMGMAQARQVIGQLEHESSQHVPFANEMAELLFGQLVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRHLNSQKD
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA094/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA094/annotation5/range/1

https://synbiohub.org/public/SEGA/SEGA094/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA094/1
start: 1899
end: 2744
orientation: reverseComplement

pstS
type: SequenceAnnotation
persistent identity: annotation16
display id: annotation16
version: 1
codon start: 1
label: pstS
note: pstS
translation: MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKWADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA094/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA094/annotation16/range/1

https://synbiohub.org/public/SEGA/SEGA094/annotation16/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA094/1
start: 6579
end: 6826
orientation: inline

L3S3P22 Terminator (synthetic)
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
label: L3S3P22 Terminator (synthetic)
note: L3S3P22 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA094/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA094/annotation12/range/1

https://synbiohub.org/public/SEGA/SEGA094/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA094/1
start: 5016
end: 5064
orientation: inline

medium TIR
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
note: TIR
note: medium TIR
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA094/1
role: SO:0000139
location: https://synbiohub.org/public/SEGA/SEGA094/annotation8/range/1

https://synbiohub.org/public/SEGA/SEGA094/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA094/1
start: 3930
end: 3956
orientation: inline

YebF
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
codon start: 1
label: YebF
note: YebF
product: full size yebF protein
translation: ANNETSKSVTFPKCEDLDAAGIAASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHYQVSVDCKAGMAEYQRR
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA094/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA094/annotation10/range/1

https://synbiohub.org/public/SEGA/SEGA094/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA094/1
start: 4011
end: 4301
orientation: inline

TcR
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
codon start: 1
gene: tet
label: TcR
note: TcR
note: confers resistance to tetracycline
product: tetracycline efflux protein
translation: MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHYGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA094/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA094/annotation14/range/1

https://synbiohub.org/public/SEGA/SEGA094/annotation14/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA094/1
start: 5143
end: 6333
orientation: inline

https://synbiohub.org/public/SEGA/SEGA061/1
type: ComponentDefinition
persistent identity: SEGA061
display id: SEGA061
version: 1
description: natural linear DNA.
owned by: carbay
owned by: morno
date: 08-SEP-2021
division: UNA
keywords: .
locus: SEGA061
molecule: ds-DNA
organism: unspecified
reference: https://synbiohub.org/user/carbay/SEGA/SEGA061_reference0/1
source: natural DNA sequence
type: DnaRegion
type: SO:0000987
role: SO:0000804
sequence annotation: GFP
sequence annotation: lac operator
sequence annotation: lacI
sequence annotation: TcR
sequence annotation: glmS
sequence annotation: T7 promoter
sequence annotation: L3S2P11 Terminator
sequence annotation: high TIR
sequence annotation: L3S3P22 Terminator (synthetic)
sequence annotation: lacI promoter
sequence annotation: Ubiquitin
sequence annotation: Promoter 3BCD2
sequence annotation: rrnC
sequence annotation: pstS
sequence: https://synbiohub.org/public/SEGA/SEGA061_seq/1

GFP
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
codon start: 1
label: GFP
note: GFP
product: green fluorescent protein
translation: SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYK
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA061/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA061/annotation8/range/1

https://synbiohub.org/public/SEGA/SEGA061/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA061/1
start: 2177
end: 2887
orientation: inline

lac operator
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
bound moiety: lac repressor encoded by lacI
feature type: protein_bind
label: lac operator
note: The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG).
note: lac operator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA061/1
role: SO:0000110
location: https://synbiohub.org/public/SEGA/SEGA061/annotation5/range/1

https://synbiohub.org/public/SEGA/SEGA061/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA061/1
start: 1814
end: 1838
orientation: inline

lacI
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
codon start: 1
gene: lacI
label: lacI
note: The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG).
note: lacI
product: lac repressor
translation: MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA061/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA061/annotation2/range/1

https://synbiohub.org/public/SEGA/SEGA061/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA061/1
start: 604
end: 1686
orientation: reverseComplement

TcR
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
codon start: 1
gene: tet
label: TcR
note: TcR
note: confers resistance to tetracycline
product: tetracycline efflux protein
translation: MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHYGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVIFGEDRFRWSATMSGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA061/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA061/annotation11/range/1

https://synbiohub.org/public/SEGA/SEGA061/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA061/1
start: 3018
end: 4208
orientation: inline

glmS
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
codon start: 1
label: glmS
note: glmS
translation: VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA061/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA061/annotation0/range/1

https://synbiohub.org/public/SEGA/SEGA061/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA061/1
start: 1
end: 528
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
label: T7 promoter
note: T7 promoter
note: promoter for bacteriophage T7 RNA polymerase
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA061/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA061/annotation4/range/1

https://synbiohub.org/public/SEGA/SEGA061/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA061/1
start: 1795
end: 1813
orientation: inline

L3S2P11 Terminator
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
label: L3S2P11 Terminator
note: L3S2P11 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA061/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA061/annotation1/range/1

https://synbiohub.org/public/SEGA/SEGA061/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA061/1
start: 538
end: 594
orientation: inline

high TIR
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
note: TIR
note: high TIR
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA061/1
role: SO:0000139
location: https://synbiohub.org/public/SEGA/SEGA061/annotation6/range/1

https://synbiohub.org/public/SEGA/SEGA061/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA061/1
start: 1850
end: 1876
orientation: inline

L3S3P22 Terminator (synthetic)
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
label: L3S3P22 Terminator (synthetic)
note: L3S3P22 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA061/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA061/annotation9/range/1

https://synbiohub.org/public/SEGA/SEGA061/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA061/1
start: 2891
end: 2939
orientation: inline

lacI promoter
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
gene: lacI
label: lacI promoter
note: lacI promoter
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA061/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA061/annotation3/range/1

https://synbiohub.org/public/SEGA/SEGA061/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA061/1
start: 1687
end: 1764
orientation: reverseComplement

Ubiquitin
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
codon start: 1
label: Ubiquitin
note: Ubiquitin
translation: MQIFVKTLTGKTITLEVEPGSAHHHHHHHHHHAGSSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQEKSTLELVLELQNETQSATSDA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA061/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA061/annotation7/range/1

https://synbiohub.org/public/SEGA/SEGA061/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA061/1
start: 1868
end: 2167
orientation: inline

Promoter 3BCD2
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
label: Promoter 3BCD2
note: Promoter 3BCD2
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA061/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA061/annotation10/range/1

https://synbiohub.org/public/SEGA/SEGA061/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA061/1
start: 2950
end: 2978
orientation: inline

rrnC
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
label: rrnC
note: rrnC
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA061/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA061/annotation12/range/1

https://synbiohub.org/public/SEGA/SEGA061/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA061/1
start: 4219
end: 4259
orientation: inline

pstS
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
codon start: 1
label: pstS
note: pstS
translation: MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKWADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA061/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA061/annotation13/range/1

https://synbiohub.org/public/SEGA/SEGA061/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA061/1
start: 4454
end: 4701
orientation: inline

https://synbiohub.org/public/SEGA/SEGA028/1
type: ComponentDefinition
persistent identity: SEGA028
display id: SEGA028
version: 1
description: natural linear DNA.
owned by: carbay
owned by: morno
date: 09-SEP-2021
division: UNA
keywords: .
locus: SEGA028
molecule: ds-DNA
organism: unspecified
reference: https://synbiohub.org/user/carbay/SEGA/SEGA028_reference0/1
source: natural DNA sequence
type: DnaRegion
type: SO:0000987
role: SO:0000804
sequence annotation: L3S2P11 Terminator
sequence annotation: pstS
sequence annotation: Promoter 3BCD2
sequence annotation: TcR
sequence annotation: lacI promoter
sequence annotation: lacO
sequence annotation: lacI
sequence annotation: GFP
sequence annotation: The trc promoter combines the -35 region from the trp promoter and the -10 region from the lacUV5 promoter
sequence annotation: glmS
sequence annotation: rrnC
sequence annotation: high TIR
sequence annotation: TEV site
sequence annotation: L3S3P22 Terminator (synthetic)
sequence: https://synbiohub.org/public/SEGA/SEGA028_seq/1

L3S2P11 Terminator
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
label: L3S2P11 Terminator
note: L3S2P11 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA028/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA028/annotation1/range/1

https://synbiohub.org/public/SEGA/SEGA028/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA028/1
start: 538
end: 594
orientation: inline

pstS
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
codon start: 1
label: pstS
note: pstS
translation: MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKWADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA028/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA028/annotation13/range/1

https://synbiohub.org/public/SEGA/SEGA028/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA028/1
start: 4176
end: 4423
orientation: inline

Promoter 3BCD2
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
label: Promoter 3BCD2
note: Promoter 3BCD2
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA028/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA028/annotation10/range/1

https://synbiohub.org/public/SEGA/SEGA028/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA028/1
start: 2672
end: 2700
orientation: inline

TcR
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
codon start: 1
gene: tet
label: TcR
note: TcR
note: confers resistance to tetracycline
product: tetracycline efflux protein
translation: MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHYGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVIFGEDRFRWSATMSGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA028/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA028/annotation11/range/1

https://synbiohub.org/public/SEGA/SEGA028/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA028/1
start: 2740
end: 3930
orientation: inline

lacI promoter
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
gene: lacI
label: lacI promoter
note: lacI promoter
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA028/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA028/annotation3/range/1

https://synbiohub.org/public/SEGA/SEGA028/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA028/1
start: 1687
end: 1764
orientation: reverseComplement

lacO
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
bound moiety: lac repressor
feature type: protein_bind
label: lacO
note: lac operator
note: lacO
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA028/1
role: SO:0000110
location: https://synbiohub.org/public/SEGA/SEGA028/annotation5/range/1

https://synbiohub.org/public/SEGA/SEGA028/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA028/1
start: 1825
end: 1848
orientation: inline

lacI
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
codon start: 1
gene: lacI
label: lacI
note: The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG).
note: lacI
product: lac repressor
translation: MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA028/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA028/annotation2/range/1

https://synbiohub.org/public/SEGA/SEGA028/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA028/1
start: 604
end: 1686
orientation: reverseComplement

GFP
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
codon start: 1
label: GFP
note: GFP
product: green fluorescent protein
translation: SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSTLSKDPNEKRDHMVLLEFVTAAGITHGMDELYK
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA028/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA028/annotation8/range/1

https://synbiohub.org/public/SEGA/SEGA028/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA028/1
start: 1899
end: 2609
orientation: inline

The trc promoter combines the -35 region from the trp promoter and the -10 region from the lacUV5 promoter
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
note: Ptrc
note: The trc promoter combines the -35 region from the trp promoter and the -10 region from the lacUV5 promoter
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA028/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA028/annotation4/range/1

https://synbiohub.org/public/SEGA/SEGA028/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA028/1
start: 1795
end: 1824
orientation: inline

glmS
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
codon start: 1
label: glmS
note: glmS
translation: VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA028/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA028/annotation0/range/1

https://synbiohub.org/public/SEGA/SEGA028/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA028/1
start: 1
end: 528
orientation: inline

rrnC
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
label: rrnC
note: rrnC
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA028/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA028/annotation12/range/1

https://synbiohub.org/public/SEGA/SEGA028/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA028/1
start: 3941
end: 3981
orientation: inline

high TIR
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
note: TIR
note: high TIR
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA028/1
role: SO:0000139
location: https://synbiohub.org/public/SEGA/SEGA028/annotation6/range/1

https://synbiohub.org/public/SEGA/SEGA028/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA028/1
start: 1860
end: 1886
orientation: inline

TEV site
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
codon start: 1
label: TEV site
note: TEV site
product: tobacco etch virus (TEV) protease recognition and cleavage site
translation: MENLYFQ
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA028/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA028/annotation7/range/1

https://synbiohub.org/public/SEGA/SEGA028/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA028/1
start: 1878
end: 1898
orientation: inline

L3S3P22 Terminator (synthetic)
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
label: L3S3P22 Terminator (synthetic)
note: L3S3P22 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA028/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA028/annotation9/range/1

https://synbiohub.org/public/SEGA/SEGA028/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA028/1
start: 2613
end: 2661
orientation: inline

https://synbiohub.org/public/SEGA/SEGA048/1
type: ComponentDefinition
persistent identity: SEGA048
display id: SEGA048
version: 1
description: natural linear DNA.
owned by: carbay
owned by: morno
date: 08-SEP-2021
division: UNA
keywords: .
locus: SEGA048
molecule: ds-DNA
organism: unspecified
reference: https://synbiohub.org/public/SEGA/reference0_1/1
source: natural DNA sequence
type: DnaRegion
type: SO:0000987
role: SO:0000804
sequence annotation: L3S2P11 Terminator
sequence annotation: glmS
sequence annotation: rrnC
sequence annotation: L3S3P22 Terminator (synthetic)
sequence annotation: medium TIR
sequence annotation: TcR
sequence annotation: TEV site
sequence annotation: pstS
sequence annotation: rhaB promoter
sequence annotation: PROTi-Linker
sequence annotation: Promoter 3BCD2
sequence annotation: rhaS
sequence annotation: GFP
sequence annotation: rhaR
sequence: https://synbiohub.org/public/SEGA/SEGA048_seq/1

L3S2P11 Terminator
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
label: L3S2P11 Terminator
note: L3S2P11 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA048/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA048/annotation1/range/1

https://synbiohub.org/public/SEGA/SEGA048/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA048/1
start: 538
end: 594
orientation: inline

glmS
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
codon start: 1
label: glmS
note: glmS
translation: VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA048/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA048/annotation0/range/1

https://synbiohub.org/public/SEGA/SEGA048/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA048/1
start: 1
end: 528
orientation: inline

rrnC
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
label: rrnC
note: rrnC
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA048/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA048/annotation12/range/1

https://synbiohub.org/public/SEGA/SEGA048/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA048/1
start: 4755
end: 4795
orientation: inline

L3S3P22 Terminator (synthetic)
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
label: L3S3P22 Terminator (synthetic)
note: L3S3P22 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA048/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA048/annotation9/range/1

https://synbiohub.org/public/SEGA/SEGA048/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA048/1
start: 3427
end: 3475
orientation: inline

medium TIR
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
note: TIR
note: medium TIR
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA048/1
role: SO:0000139
location: https://synbiohub.org/public/SEGA/SEGA048/annotation5/range/1

https://synbiohub.org/public/SEGA/SEGA048/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA048/1
start: 2638
end: 2664
orientation: inline

TcR
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
codon start: 1
gene: tet
label: TcR
note: TcR
note: confers resistance to tetracycline
product: tetracycline efflux protein
translation: MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHYGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVIFGEDRFRWSATMSGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA048/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA048/annotation11/range/1

https://synbiohub.org/public/SEGA/SEGA048/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA048/1
start: 3554
end: 4744
orientation: inline

TEV site
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
codon start: 1
label: TEV site
note: TEV site
product: tobacco etch virus (TEV) protease recognition and cleavage site
translation: MENLYFQ
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA048/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA048/annotation6/range/1

https://synbiohub.org/public/SEGA/SEGA048/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA048/1
start: 2656
end: 2676
orientation: inline

pstS
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
codon start: 1
label: pstS
note: pstS
translation: MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKWADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA048/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA048/annotation13/range/1

https://synbiohub.org/public/SEGA/SEGA048/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA048/1
start: 4990
end: 5237
orientation: inline

rhaB promoter
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
label: rhaB promoter
note: promoter of the E. coli rhaBAD operon, conferring tight induction with L-rhamnose and repression with D-glucose in the presence of RhaR and RhaS (Giacalone et al., 2006)
note: rhaB promoter
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA048/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA048/annotation4/range/1

https://synbiohub.org/public/SEGA/SEGA048/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA048/1
start: 2508
end: 2626
orientation: inline

PROTi-Linker
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
label: PROTi-Linker
note: PROTi-Linker
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA048/1
role: SO:0001411
location: https://synbiohub.org/public/SEGA/SEGA048/annotation7/range/1

https://synbiohub.org/public/SEGA/SEGA048/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA048/1
start: 2677
end: 2712
orientation: inline

Promoter 3BCD2
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
label: Promoter 3BCD2
note: Promoter 3BCD2
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA048/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA048/annotation10/range/1

https://synbiohub.org/public/SEGA/SEGA048/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA048/1
start: 3486
end: 3514
orientation: inline

rhaS
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
codon start: 1
gene: E. coli rhaS
label: rhaS
note: activated by L-rhamnose
note: rhaS
product: positive regulator of the rhaB promoter
translation: MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHGTGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLLPQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKSSLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQRYLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGFLQ
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA048/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA048/annotation3/range/1

https://synbiohub.org/public/SEGA/SEGA048/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA048/1
start: 1526
end: 2362
orientation: reverseComplement

GFP
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
codon start: 1
label: GFP
note: GFP
product: green fluorescent protein
translation: SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYK
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA048/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA048/annotation8/range/1

https://synbiohub.org/public/SEGA/SEGA048/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA048/1
start: 2713
end: 3423
orientation: inline

rhaR
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
codon start: 1
gene: E. coli rhaR
label: rhaR
note: needed for rhamnose-dependent activation of the rhaB promoter
note: rhaR
product: transcriptional activator of rhaR and rhaS
translation: MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQGAIPGFNASAGQPHWRLGSMGMAQARQVIGQLEHESSQHVPFANEMAELLFGQLVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRHLNSQKD
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA048/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA048/annotation2/range/1

https://synbiohub.org/public/SEGA/SEGA048/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA048/1
start: 607
end: 1452
orientation: reverseComplement

https://synbiohub.org/public/SEGA/SEGA059/1
type: ComponentDefinition
persistent identity: SEGA059
display id: SEGA059
version: 1
description: natural linear DNA.
owned by: carbay
owned by: morno
date: 08-SEP-2021
division: UNA
keywords: .
locus: SEGA059
molecule: ds-DNA
organism: unspecified
reference: https://synbiohub.org/public/SEGA/reference0_1/1
source: natural DNA sequence
type: DnaRegion
type: SO:0000987
role: SO:0000804
sequence annotation: lacI promoter
sequence annotation: low TIR
sequence annotation: T7 promoter
sequence annotation: Promoter 3BCD2
sequence annotation: lacI
sequence annotation: GFP
sequence annotation: L3S2P11 Terminator
sequence annotation: rrnC
sequence annotation: TcR
sequence annotation: glmS
sequence annotation: lac operator
sequence annotation: L3S3P22 Terminator (synthetic)
sequence annotation: pstS
sequence annotation: Ubiquitin
sequence: https://synbiohub.org/public/SEGA/SEGA059_seq/1

lacI promoter
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
gene: lacI
label: lacI promoter
note: lacI promoter
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA059/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA059/annotation3/range/1

https://synbiohub.org/public/SEGA/SEGA059/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA059/1
start: 1687
end: 1764
orientation: reverseComplement

low TIR
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
note: TIR
note: low TIR
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA059/1
role: SO:0000139
location: https://synbiohub.org/public/SEGA/SEGA059/annotation6/range/1

https://synbiohub.org/public/SEGA/SEGA059/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA059/1
start: 1850
end: 1876
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
label: T7 promoter
note: T7 promoter
note: promoter for bacteriophage T7 RNA polymerase
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA059/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA059/annotation4/range/1

https://synbiohub.org/public/SEGA/SEGA059/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA059/1
start: 1795
end: 1813
orientation: inline

Promoter 3BCD2
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
label: Promoter 3BCD2
note: Promoter 3BCD2
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA059/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA059/annotation10/range/1

https://synbiohub.org/public/SEGA/SEGA059/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA059/1
start: 2950
end: 2978
orientation: inline

lacI
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
codon start: 1
gene: lacI
label: lacI
note: The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG).
note: lacI
product: lac repressor
translation: MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA059/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA059/annotation2/range/1

https://synbiohub.org/public/SEGA/SEGA059/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA059/1
start: 604
end: 1686
orientation: reverseComplement

GFP
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
codon start: 1
label: GFP
note: GFP
product: green fluorescent protein
translation: SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYK
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA059/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA059/annotation8/range/1

https://synbiohub.org/public/SEGA/SEGA059/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA059/1
start: 2177
end: 2887
orientation: inline

L3S2P11 Terminator
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
label: L3S2P11 Terminator
note: L3S2P11 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA059/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA059/annotation1/range/1

https://synbiohub.org/public/SEGA/SEGA059/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA059/1
start: 538
end: 594
orientation: inline

rrnC
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
label: rrnC
note: rrnC
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA059/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA059/annotation12/range/1

https://synbiohub.org/public/SEGA/SEGA059/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA059/1
start: 4219
end: 4259
orientation: inline

TcR
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
codon start: 1
gene: tet
label: TcR
note: TcR
note: confers resistance to tetracycline
product: tetracycline efflux protein
translation: MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHYGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVIFGEDRFRWSATMSGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA059/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA059/annotation11/range/1

https://synbiohub.org/public/SEGA/SEGA059/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA059/1
start: 3018
end: 4208
orientation: inline

glmS
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
codon start: 1
label: glmS
note: glmS
translation: VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA059/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA059/annotation0/range/1

https://synbiohub.org/public/SEGA/SEGA059/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA059/1
start: 1
end: 528
orientation: inline

lac operator
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
bound moiety: lac repressor encoded by lacI
feature type: protein_bind
label: lac operator
note: The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG).
note: lac operator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA059/1
role: SO:0000110
location: https://synbiohub.org/public/SEGA/SEGA059/annotation5/range/1

https://synbiohub.org/public/SEGA/SEGA059/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA059/1
start: 1814
end: 1838
orientation: inline

L3S3P22 Terminator (synthetic)
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
label: L3S3P22 Terminator (synthetic)
note: L3S3P22 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA059/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA059/annotation9/range/1

https://synbiohub.org/public/SEGA/SEGA059/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA059/1
start: 2891
end: 2939
orientation: inline

pstS
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
codon start: 1
label: pstS
note: pstS
translation: MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKWADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA059/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA059/annotation13/range/1

https://synbiohub.org/public/SEGA/SEGA059/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA059/1
start: 4454
end: 4701
orientation: inline

Ubiquitin
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
codon start: 1
label: Ubiquitin
note: Ubiquitin
translation: MQIFVKTLTGKTITLEVEPGSAHHHHHHHHHHAGSSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQEKSTLELVLELQNETQSATSDA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA059/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA059/annotation7/range/1

https://synbiohub.org/public/SEGA/SEGA059/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA059/1
start: 1868
end: 2167
orientation: inline

https://synbiohub.org/public/SEGA/SEGA058/1
type: ComponentDefinition
persistent identity: SEGA058
display id: SEGA058
version: 1
description: natural linear DNA.
owned by: carbay
owned by: morno
date: 08-SEP-2021
division: UNA
keywords: .
locus: SEGA058
molecule: ds-DNA
organism: unspecified
reference: https://synbiohub.org/user/carbay/SEGA/SEGA058_reference0/1
source: natural DNA sequence
type: DnaRegion
type: SO:0000987
role: SO:0000804
sequence annotation: L3S3P22 Terminator (synthetic)
sequence annotation: lacI promoter
sequence annotation: high TIR
sequence annotation: pstS
sequence annotation: lac operator
sequence annotation: T7 promoter
sequence annotation: Promoter 3BCD2
sequence annotation: glmS
sequence annotation: GFP
sequence annotation: TcR
sequence annotation: BCD
sequence annotation: lacI
sequence annotation: rrnC
sequence annotation: L3S2P11 Terminator
sequence: https://synbiohub.org/public/SEGA/SEGA058_seq/1

L3S3P22 Terminator (synthetic)
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
label: L3S3P22 Terminator (synthetic)
note: L3S3P22 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA058/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA058/annotation9/range/1

https://synbiohub.org/public/SEGA/SEGA058/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA058/1
start: 2638
end: 2686
orientation: inline

lacI promoter
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
gene: lacI
label: lacI promoter
note: lacI promoter
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA058/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA058/annotation3/range/1

https://synbiohub.org/public/SEGA/SEGA058/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA058/1
start: 1687
end: 1764
orientation: reverseComplement

high TIR
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
note: TIR
note: high TIR
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA058/1
role: SO:0000139
location: https://synbiohub.org/public/SEGA/SEGA058/annotation6/range/1

https://synbiohub.org/public/SEGA/SEGA058/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA058/1
start: 1850
end: 1876
orientation: inline

pstS
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
codon start: 1
label: pstS
note: pstS
translation: MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKWADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA058/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA058/annotation13/range/1

https://synbiohub.org/public/SEGA/SEGA058/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA058/1
start: 4201
end: 4448
orientation: inline

lac operator
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
bound moiety: lac repressor encoded by lacI
feature type: protein_bind
label: lac operator
note: The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG).
note: lac operator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA058/1
role: SO:0000110
location: https://synbiohub.org/public/SEGA/SEGA058/annotation5/range/1

https://synbiohub.org/public/SEGA/SEGA058/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA058/1
start: 1814
end: 1838
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
label: T7 promoter
note: T7 promoter
note: promoter for bacteriophage T7 RNA polymerase
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA058/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA058/annotation4/range/1

https://synbiohub.org/public/SEGA/SEGA058/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA058/1
start: 1795
end: 1813
orientation: inline

Promoter 3BCD2
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
label: Promoter 3BCD2
note: Promoter 3BCD2
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA058/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA058/annotation10/range/1

https://synbiohub.org/public/SEGA/SEGA058/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA058/1
start: 2697
end: 2725
orientation: inline

glmS
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
codon start: 1
label: glmS
note: glmS
translation: VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA058/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA058/annotation0/range/1

https://synbiohub.org/public/SEGA/SEGA058/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA058/1
start: 1
end: 528
orientation: inline

GFP
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
codon start: 1
label: GFP
note: GFP
product: green fluorescent protein
translation: SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYK
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA058/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA058/annotation8/range/1

https://synbiohub.org/public/SEGA/SEGA058/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA058/1
start: 1924
end: 2634
orientation: inline

TcR
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
codon start: 1
gene: tet
label: TcR
note: TcR
note: confers resistance to tetracycline
product: tetracycline efflux protein
translation: MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHYGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVIFGEDRFRWSATMSGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA058/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA058/annotation11/range/1

https://synbiohub.org/public/SEGA/SEGA058/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA058/1
start: 2765
end: 3955
orientation: inline

BCD
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
codon start: 1
label: BCD
note: BCD
translation: MKAIFVLKHLNHAKEVF
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA058/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA058/annotation7/range/1

https://synbiohub.org/public/SEGA/SEGA058/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA058/1
start: 1868
end: 1923
orientation: inline

lacI
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
codon start: 1
gene: lacI
label: lacI
note: The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG).
note: lacI
product: lac repressor
translation: MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA058/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA058/annotation2/range/1

https://synbiohub.org/public/SEGA/SEGA058/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA058/1
start: 604
end: 1686
orientation: reverseComplement

rrnC
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
label: rrnC
note: rrnC
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA058/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA058/annotation12/range/1

https://synbiohub.org/public/SEGA/SEGA058/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA058/1
start: 3966
end: 4006
orientation: inline

L3S2P11 Terminator
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
label: L3S2P11 Terminator
note: L3S2P11 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA058/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA058/annotation1/range/1

https://synbiohub.org/public/SEGA/SEGA058/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA058/1
start: 538
end: 594
orientation: inline

https://synbiohub.org/public/SEGA/SEGA062/1
type: ComponentDefinition
persistent identity: SEGA062
display id: SEGA062
version: 1
description: natural linear DNA.
owned by: carbay
owned by: morno
date: 09-SEP-2021
division: UNA
keywords: .
locus: SEGA062
molecule: ds-DNA
organism: unspecified
reference: https://synbiohub.org/user/carbay/SEGA/SEGA062_reference0/1
source: natural DNA sequence
type: DnaRegion
type: SO:0000987
role: SO:0000804
sequence annotation: glmS
sequence annotation: T7 promoter
sequence annotation: TEV site
sequence annotation: L3S2P11 Terminator
sequence annotation: GFP
sequence annotation: pstS
sequence annotation: lacI
sequence annotation: rrnC
sequence annotation: L3S3P22 Terminator (synthetic)
sequence annotation: lacI promoter
sequence annotation: lac operator
sequence annotation: TcR
sequence annotation: Promoter 3BCD2
sequence annotation: low TIR
sequence: https://synbiohub.org/public/SEGA/SEGA062_seq/1

glmS
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
codon start: 1
label: glmS
note: glmS
translation: VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA062/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA062/annotation0/range/1

https://synbiohub.org/public/SEGA/SEGA062/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA062/1
start: 1
end: 528
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
label: T7 promoter
note: T7 promoter
note: promoter for bacteriophage T7 RNA polymerase
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA062/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA062/annotation4/range/1

https://synbiohub.org/public/SEGA/SEGA062/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA062/1
start: 1795
end: 1813
orientation: inline

TEV site
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
codon start: 1
label: TEV site
note: TEV site
product: tobacco etch virus (TEV) protease recognition and cleavage site
translation: MENLYFQ
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA062/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA062/annotation7/range/1

https://synbiohub.org/public/SEGA/SEGA062/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA062/1
start: 1868
end: 1888
orientation: inline

L3S2P11 Terminator
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
label: L3S2P11 Terminator
note: L3S2P11 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA062/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA062/annotation1/range/1

https://synbiohub.org/public/SEGA/SEGA062/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA062/1
start: 538
end: 594
orientation: inline

GFP
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
codon start: 1
label: GFP
note: GFP
product: green fluorescent protein
translation: SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYK
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA062/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA062/annotation8/range/1

https://synbiohub.org/public/SEGA/SEGA062/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA062/1
start: 1889
end: 2599
orientation: inline

pstS
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
codon start: 1
label: pstS
note: pstS
translation: MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKWADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA062/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA062/annotation13/range/1

https://synbiohub.org/public/SEGA/SEGA062/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA062/1
start: 4166
end: 4413
orientation: inline

lacI
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
codon start: 1
gene: lacI
label: lacI
note: The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG).
note: lacI
product: lac repressor
translation: MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA062/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA062/annotation2/range/1

https://synbiohub.org/public/SEGA/SEGA062/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA062/1
start: 604
end: 1686
orientation: reverseComplement

rrnC
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
label: rrnC
note: rrnC
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA062/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA062/annotation12/range/1

https://synbiohub.org/public/SEGA/SEGA062/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA062/1
start: 3931
end: 3971
orientation: inline

L3S3P22 Terminator (synthetic)
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
label: L3S3P22 Terminator (synthetic)
note: L3S3P22 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA062/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA062/annotation9/range/1

https://synbiohub.org/public/SEGA/SEGA062/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA062/1
start: 2603
end: 2651
orientation: inline

lacI promoter
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
gene: lacI
label: lacI promoter
note: lacI promoter
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA062/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA062/annotation3/range/1

https://synbiohub.org/public/SEGA/SEGA062/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA062/1
start: 1687
end: 1764
orientation: reverseComplement

lac operator
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
bound moiety: lac repressor encoded by lacI
feature type: protein_bind
label: lac operator
note: The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG).
note: lac operator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA062/1
role: SO:0000110
location: https://synbiohub.org/public/SEGA/SEGA062/annotation5/range/1

https://synbiohub.org/public/SEGA/SEGA062/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA062/1
start: 1814
end: 1838
orientation: inline

TcR
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
codon start: 1
gene: tet
label: TcR
note: TcR
note: confers resistance to tetracycline
product: tetracycline efflux protein
translation: MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHYGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVIFGEDRFRWSATMSGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA062/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA062/annotation11/range/1

https://synbiohub.org/public/SEGA/SEGA062/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA062/1
start: 2730
end: 3920
orientation: inline

Promoter 3BCD2
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
label: Promoter 3BCD2
note: Promoter 3BCD2
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA062/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA062/annotation10/range/1

https://synbiohub.org/public/SEGA/SEGA062/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA062/1
start: 2662
end: 2690
orientation: inline

low TIR
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
note: TIR
note: low TIR
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA062/1
role: SO:0000139
location: https://synbiohub.org/public/SEGA/SEGA062/annotation6/range/1

https://synbiohub.org/public/SEGA/SEGA062/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA062/1
start: 1850
end: 1876
orientation: inline

https://synbiohub.org/public/SEGA/SEGA073/1
type: ComponentDefinition
persistent identity: SEGA073
display id: SEGA073
version: 1
description: natural linear DNA.
owned by: carbay
owned by: morno
date: 09-SEP-2021
division: UNA
keywords: .
locus: SEGA073
molecule: ds-DNA
organism: unspecified
reference: https://synbiohub.org/public/SEGA/reference0_1/1
source: natural DNA sequence
type: DnaRegion
type: SO:0000987
role: SO:0000804
sequence annotation: lacI promoter
sequence annotation: glmS
sequence annotation: TcR
sequence annotation: lacI
sequence annotation: L3S3P22 Terminator (synthetic)
sequence annotation: L3S2P11 Terminator
sequence annotation: YebF
sequence annotation: Promoter 3BCD2
sequence annotation: yebF
sequence annotation: lac operator
sequence annotation: high TIR
sequence annotation: sfGFP
sequence annotation: pstS
sequence annotation: T7 promoter
sequence annotation: rrnC
sequence: https://synbiohub.org/public/SEGA/SEGA073_seq/1

lacI promoter
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
gene: lacI
label: lacI promoter
note: lacI promoter
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA073/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA073/annotation3/range/1

https://synbiohub.org/public/SEGA/SEGA073/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA073/1
start: 1687
end: 1764
orientation: reverseComplement

glmS
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
codon start: 1
label: glmS
note: glmS
translation: VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA073/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA073/annotation0/range/1

https://synbiohub.org/public/SEGA/SEGA073/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA073/1
start: 1
end: 528
orientation: inline

TcR
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
codon start: 1
gene: tet
label: TcR
note: TcR
note: confers resistance to tetracycline
product: tetracycline efflux protein
translation: MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHYGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVIFGEDRFRWSATMSGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA073/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA073/annotation12/range/1

https://synbiohub.org/public/SEGA/SEGA073/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA073/1
start: 3063
end: 4253
orientation: inline

lacI
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
codon start: 1
gene: lacI
label: lacI
note: The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG).
note: lacI
product: lac repressor
translation: MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA073/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA073/annotation2/range/1

https://synbiohub.org/public/SEGA/SEGA073/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA073/1
start: 604
end: 1686
orientation: reverseComplement

L3S3P22 Terminator (synthetic)
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
label: L3S3P22 Terminator (synthetic)
note: L3S3P22 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA073/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA073/annotation10/range/1

https://synbiohub.org/public/SEGA/SEGA073/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA073/1
start: 2936
end: 2984
orientation: inline

L3S2P11 Terminator
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
label: L3S2P11 Terminator
note: L3S2P11 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA073/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA073/annotation1/range/1

https://synbiohub.org/public/SEGA/SEGA073/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA073/1
start: 538
end: 594
orientation: inline

YebF
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
codon start: 1
label: YebF
note: YebF
product: full size yebF protein
translation: ANNETSKSVTFPKCEDLDAAGIAASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHYQVSVDCKAGMAEYQRR
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA073/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA073/annotation8/range/1

https://synbiohub.org/public/SEGA/SEGA073/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA073/1
start: 1931
end: 2221
orientation: inline

Promoter 3BCD2
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
label: Promoter 3BCD2
note: Promoter 3BCD2
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA073/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA073/annotation11/range/1

https://synbiohub.org/public/SEGA/SEGA073/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA073/1
start: 2995
end: 3023
orientation: inline

yebF
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
codon start: 1
db xref: ASAP:ABE-0006141
db xref: EcoGene:EG11807
db xref: GI:145698278
db xref: GeneID:946363
db xref: UniProtKB/Swiss-Prot:P33219
gene: yebF
gene synonym: ECK1848; JW1836
label: yebF
locus tag: b1847
note: yebFss
product: yebF signal sequence
protein id: NP_416361.2
translation: MKKRGAFLGLLLVSACASVFA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA073/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA073/annotation7/range/1

https://synbiohub.org/public/SEGA/SEGA073/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA073/1
start: 1868
end: 1930
orientation: inline

lac operator
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
bound moiety: lac repressor encoded by lacI
feature type: protein_bind
label: lac operator
note: The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG).
note: lac operator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA073/1
role: SO:0000110
location: https://synbiohub.org/public/SEGA/SEGA073/annotation5/range/1

https://synbiohub.org/public/SEGA/SEGA073/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA073/1
start: 1814
end: 1838
orientation: inline

high TIR
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
note: TIR
note: high TIR
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA073/1
role: SO:0000139
location: https://synbiohub.org/public/SEGA/SEGA073/annotation6/range/1

https://synbiohub.org/public/SEGA/SEGA073/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA073/1
start: 1850
end: 1876
orientation: inline

sfGFP
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
codon start: 1
label: sfGFP
note: sfGFP
product: superfolder GFP
translation: RKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDGTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEFVTAAGITHGMDELYK
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA073/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA073/annotation9/range/1

https://synbiohub.org/public/SEGA/SEGA073/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA073/1
start: 2222
end: 2932
orientation: inline

pstS
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
codon start: 1
label: pstS
note: pstS
translation: MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKWADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA073/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA073/annotation14/range/1

https://synbiohub.org/public/SEGA/SEGA073/annotation14/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA073/1
start: 4499
end: 4746
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
label: T7 promoter
note: T7 promoter
note: promoter for bacteriophage T7 RNA polymerase
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA073/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA073/annotation4/range/1

https://synbiohub.org/public/SEGA/SEGA073/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA073/1
start: 1795
end: 1813
orientation: inline

rrnC
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
label: rrnC
note: rrnC
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA073/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA073/annotation13/range/1

https://synbiohub.org/public/SEGA/SEGA073/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA073/1
start: 4264
end: 4304
orientation: inline

https://synbiohub.org/public/SEGA/SEGA060/1
type: ComponentDefinition
persistent identity: SEGA060
display id: SEGA060
version: 1
description: natural linear DNA.
owned by: carbay
owned by: morno
date: 08-SEP-2021
division: UNA
keywords: .
locus: SEGA060
molecule: ds-DNA
organism: unspecified
reference: https://synbiohub.org/user/carbay/SEGA/SEGA060_reference0/1
source: natural DNA sequence
type: DnaRegion
type: SO:0000987
role: SO:0000804
sequence annotation: GFP
sequence annotation: rrnC
sequence annotation: L3S2P11 Terminator
sequence annotation: L3S3P22 Terminator (synthetic)
sequence annotation: pstS
sequence annotation: lacI promoter
sequence annotation: TcR
sequence annotation: T7 promoter
sequence annotation: Ubiquitin
sequence annotation: medium TIR
sequence annotation: lacI
sequence annotation: lac operator
sequence annotation: glmS
sequence annotation: Promoter 3BCD2
sequence: https://synbiohub.org/public/SEGA/SEGA060_seq/1

GFP
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
codon start: 1
label: GFP
note: GFP
product: green fluorescent protein
translation: SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYK
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA060/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA060/annotation8/range/1

https://synbiohub.org/public/SEGA/SEGA060/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA060/1
start: 2177
end: 2887
orientation: inline

rrnC
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
label: rrnC
note: rrnC
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA060/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA060/annotation12/range/1

https://synbiohub.org/public/SEGA/SEGA060/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA060/1
start: 4219
end: 4259
orientation: inline

L3S2P11 Terminator
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
label: L3S2P11 Terminator
note: L3S2P11 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA060/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA060/annotation1/range/1

https://synbiohub.org/public/SEGA/SEGA060/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA060/1
start: 538
end: 594
orientation: inline

L3S3P22 Terminator (synthetic)
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
label: L3S3P22 Terminator (synthetic)
note: L3S3P22 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA060/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA060/annotation9/range/1

https://synbiohub.org/public/SEGA/SEGA060/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA060/1
start: 2891
end: 2939
orientation: inline

pstS
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
codon start: 1
label: pstS
note: pstS
translation: MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKWADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA060/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA060/annotation13/range/1

https://synbiohub.org/public/SEGA/SEGA060/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA060/1
start: 4454
end: 4701
orientation: inline

lacI promoter
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
gene: lacI
label: lacI promoter
note: lacI promoter
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA060/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA060/annotation3/range/1

https://synbiohub.org/public/SEGA/SEGA060/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA060/1
start: 1687
end: 1764
orientation: reverseComplement

TcR
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
codon start: 1
gene: tet
label: TcR
note: TcR
note: confers resistance to tetracycline
product: tetracycline efflux protein
translation: MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHYGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVIFGEDRFRWSATMSGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA060/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA060/annotation11/range/1

https://synbiohub.org/public/SEGA/SEGA060/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA060/1
start: 3018
end: 4208
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
label: T7 promoter
note: T7 promoter
note: promoter for bacteriophage T7 RNA polymerase
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA060/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA060/annotation4/range/1

https://synbiohub.org/public/SEGA/SEGA060/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA060/1
start: 1795
end: 1813
orientation: inline

Ubiquitin
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
codon start: 1
label: Ubiquitin
note: Ubiquitin
translation: MQIFVKTLTGKTITLEVEPGSAHHHHHHHHHHAGSSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQEKSTLELVLELQNETQSATSDA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA060/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA060/annotation7/range/1

https://synbiohub.org/public/SEGA/SEGA060/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA060/1
start: 1868
end: 2167
orientation: inline

medium TIR
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
note: TIR
note: medium TIR
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA060/1
role: SO:0000139
location: https://synbiohub.org/public/SEGA/SEGA060/annotation6/range/1

https://synbiohub.org/public/SEGA/SEGA060/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA060/1
start: 1850
end: 1876
orientation: inline

lacI
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
codon start: 1
gene: lacI
label: lacI
note: The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG).
note: lacI
product: lac repressor
translation: MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA060/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA060/annotation2/range/1

https://synbiohub.org/public/SEGA/SEGA060/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA060/1
start: 604
end: 1686
orientation: reverseComplement

lac operator
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
bound moiety: lac repressor encoded by lacI
feature type: protein_bind
label: lac operator
note: The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG).
note: lac operator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA060/1
role: SO:0000110
location: https://synbiohub.org/public/SEGA/SEGA060/annotation5/range/1

https://synbiohub.org/public/SEGA/SEGA060/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA060/1
start: 1814
end: 1838
orientation: inline

glmS
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
codon start: 1
label: glmS
note: glmS
translation: VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA060/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA060/annotation0/range/1

https://synbiohub.org/public/SEGA/SEGA060/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA060/1
start: 1
end: 528
orientation: inline

Promoter 3BCD2
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
label: Promoter 3BCD2
note: Promoter 3BCD2
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA060/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA060/annotation10/range/1

https://synbiohub.org/public/SEGA/SEGA060/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA060/1
start: 2950
end: 2978
orientation: inline

https://synbiohub.org/public/SEGA/SEGA104/1
type: ComponentDefinition
persistent identity: SEGA104
display id: SEGA104
version: 1
description: natural linear DNA.
owned by: carbay
owned by: morno
date: 09-SEP-2021
division: UNA
keywords: .
locus: SEGA104
molecule: ds-DNA
organism: unspecified
reference: https://synbiohub.org/user/carbay/SEGA/SEGA104_reference0/1
source: natural DNA sequence
type: DnaRegion
type: SO:0000987
role: SO:0000804
sequence annotation: rrnC
sequence annotation: lac operator
sequence annotation: T7 promoter
sequence annotation: BCD
sequence annotation: lacI
sequence annotation: lacI promoter
sequence annotation: high TIR
sequence annotation: glmS
sequence annotation: L3S2P11 Terminator
sequence annotation: pstS
sequence annotation: TcR
sequence: https://synbiohub.org/public/SEGA/SEGA104_seq/1

rrnC
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
label: rrnC
note: rrnC
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA104/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA104/annotation9/range/1

https://synbiohub.org/public/SEGA/SEGA104/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA104/1
start: 3047
end: 3087
orientation: inline

lac operator
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
bound moiety: lac repressor encoded by lacI
feature type: protein_bind
label: lac operator
note: The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG).
note: lac operator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA104/1
role: SO:0000110
location: https://synbiohub.org/public/SEGA/SEGA104/annotation5/range/1

https://synbiohub.org/public/SEGA/SEGA104/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA104/1
start: 1814
end: 1838
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
label: T7 promoter
note: T7 promoter
note: promoter for bacteriophage T7 RNA polymerase
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA104/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA104/annotation4/range/1

https://synbiohub.org/public/SEGA/SEGA104/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA104/1
start: 1795
end: 1813
orientation: inline

BCD
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
codon start: 1
label: BCD
note: BCD
translation: MKAIFVLKHLNHAKEVF
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA104/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA104/annotation7/range/1

https://synbiohub.org/public/SEGA/SEGA104/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA104/1
start: 1868
end: 1923
orientation: inline

lacI
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
codon start: 1
gene: lacI
label: lacI
note: The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG).
note: lacI
product: lac repressor
translation: MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA104/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA104/annotation2/range/1

https://synbiohub.org/public/SEGA/SEGA104/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA104/1
start: 604
end: 1686
orientation: reverseComplement

lacI promoter
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
gene: lacI
label: lacI promoter
note: lacI promoter
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA104/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA104/annotation3/range/1

https://synbiohub.org/public/SEGA/SEGA104/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA104/1
start: 1687
end: 1764
orientation: reverseComplement

high TIR
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
note: TIR
note: high TIR
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA104/1
role: SO:0000139
location: https://synbiohub.org/public/SEGA/SEGA104/annotation6/range/1

https://synbiohub.org/public/SEGA/SEGA104/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA104/1
start: 1850
end: 1876
orientation: inline

glmS
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
codon start: 1
label: glmS
note: glmS
translation: VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA104/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA104/annotation0/range/1

https://synbiohub.org/public/SEGA/SEGA104/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA104/1
start: 1
end: 528
orientation: inline

L3S2P11 Terminator
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
label: L3S2P11 Terminator
note: L3S2P11 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA104/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA104/annotation1/range/1

https://synbiohub.org/public/SEGA/SEGA104/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA104/1
start: 538
end: 594
orientation: inline

pstS
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
codon start: 1
label: pstS
note: pstS
translation: MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKWADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA104/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA104/annotation10/range/1

https://synbiohub.org/public/SEGA/SEGA104/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA104/1
start: 3282
end: 3529
orientation: inline

TcR
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
codon start: 1
gene: tet
label: TcR
note: TcR
note: confers resistance to tetracycline
product: tetracycline efflux protein
translation: VLPGLLRDIVHSDSIASHYGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVIFGEDRFRWSATMSGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA104/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA104/annotation8/range/1

https://synbiohub.org/public/SEGA/SEGA104/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA104/1
start: 1924
end: 3036
orientation: inline

https://synbiohub.org/public/SEGA/SEGA024/1
type: ComponentDefinition
persistent identity: SEGA024
display id: SEGA024
version: 1
description: natural linear DNA.
owned by: carbay
owned by: morno
date: 09-SEP-2021
division: UNA
keywords: .
locus: SEGA024
molecule: ds-DNA
organism: unspecified
reference: https://synbiohub.org/public/SEGA/reference0_1/1
source: natural DNA sequence
type: DnaRegion
type: SO:0000987
role: SO:0000804
sequence annotation: L3S2P11 Terminator
sequence annotation: lacI promoter
sequence annotation: rrnC
sequence annotation: lacI
sequence annotation: GFP
sequence annotation: trc promoter
sequence annotation: medium TIR
sequence annotation: lacO
sequence annotation: Ubiquitin
sequence annotation: Promoter 3BCD2
sequence annotation: TcR
sequence annotation: pstS
sequence annotation: glmS
sequence annotation: L3S3P22 Terminator (synthetic)
sequence: https://synbiohub.org/public/SEGA/SEGA024_seq/1

L3S2P11 Terminator
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
label: L3S2P11 Terminator
note: L3S2P11 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA024/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA024/annotation1/range/1

https://synbiohub.org/public/SEGA/SEGA024/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA024/1
start: 538
end: 594
orientation: inline

lacI promoter
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
gene: lacI
label: lacI promoter
note: lacI promoter
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA024/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA024/annotation3/range/1

https://synbiohub.org/public/SEGA/SEGA024/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA024/1
start: 1687
end: 1764
orientation: reverseComplement

rrnC
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
label: rrnC
note: rrnC
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA024/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA024/annotation12/range/1

https://synbiohub.org/public/SEGA/SEGA024/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA024/1
start: 4229
end: 4269
orientation: inline

lacI
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
codon start: 1
gene: lacI
label: lacI
note: The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG).
note: lacI
product: lac repressor
translation: MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA024/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA024/annotation2/range/1

https://synbiohub.org/public/SEGA/SEGA024/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA024/1
start: 604
end: 1686
orientation: reverseComplement

GFP
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
codon start: 1
label: GFP
note: GFP
product: green fluorescent protein
translation: SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYK
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA024/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA024/annotation8/range/1

https://synbiohub.org/public/SEGA/SEGA024/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA024/1
start: 2187
end: 2897
orientation: inline

trc promoter
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
label: trc promoter
note: strong E. coli promoter; hybrid between the trp and lac UV5 promoters
note: trc promoter
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA024/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA024/annotation4/range/1

https://synbiohub.org/public/SEGA/SEGA024/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA024/1
start: 1795
end: 1824
orientation: inline

medium TIR
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
note: TIR
note: medium TIR
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA024/1
role: SO:0000139
location: https://synbiohub.org/public/SEGA/SEGA024/annotation6/range/1

https://synbiohub.org/public/SEGA/SEGA024/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA024/1
start: 1860
end: 1886
orientation: inline

lacO
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
bound moiety: lac repressor
feature type: protein_bind
label: lacO
note: lac operator
note: lacO
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA024/1
role: SO:0000110
location: https://synbiohub.org/public/SEGA/SEGA024/annotation5/range/1

https://synbiohub.org/public/SEGA/SEGA024/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA024/1
start: 1825
end: 1848
orientation: inline

Ubiquitin
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
codon start: 1
label: Ubiquitin
note: Ubiquitin
translation: MQIFVKTLTGKTITLEVEPGSAHHHHHHHHHHAGSSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQEKSTLELVLELQNETQSATSDA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA024/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA024/annotation7/range/1

https://synbiohub.org/public/SEGA/SEGA024/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA024/1
start: 1878
end: 2177
orientation: inline

Promoter 3BCD2
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
label: Promoter 3BCD2
note: Promoter 3BCD2
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA024/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA024/annotation10/range/1

https://synbiohub.org/public/SEGA/SEGA024/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA024/1
start: 2960
end: 2988
orientation: inline

TcR
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
codon start: 1
gene: tet
label: TcR
note: TcR
note: confers resistance to tetracycline
product: tetracycline efflux protein
translation: MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHYGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVIFGEDRFRWSATMSGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA024/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA024/annotation11/range/1

https://synbiohub.org/public/SEGA/SEGA024/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA024/1
start: 3028
end: 4218
orientation: inline

pstS
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
codon start: 1
label: pstS
note: pstS
translation: MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKWADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA024/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA024/annotation13/range/1

https://synbiohub.org/public/SEGA/SEGA024/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA024/1
start: 4464
end: 4711
orientation: inline

glmS
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
codon start: 1
label: glmS
note: glmS
translation: VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA024/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA024/annotation0/range/1

https://synbiohub.org/public/SEGA/SEGA024/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA024/1
start: 1
end: 528
orientation: inline

L3S3P22 Terminator (synthetic)
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
label: L3S3P22 Terminator (synthetic)
note: L3S3P22 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA024/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA024/annotation9/range/1

https://synbiohub.org/public/SEGA/SEGA024/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA024/1
start: 2901
end: 2949
orientation: inline

https://synbiohub.org/public/SEGA/SEGA053/1
type: ComponentDefinition
persistent identity: SEGA053
display id: SEGA053
version: 1
description: natural linear DNA.
owned by: carbay
owned by: morno
date: 08-SEP-2021
division: UNA
keywords: .
locus: SEGA053
molecule: ds-DNA
organism: unspecified
reference: https://synbiohub.org/user/carbay/SEGA/SEGA053_reference0/1
source: natural DNA sequence
type: DnaRegion
type: SO:0000987
role: SO:0000804
sequence annotation: yebF
sequence annotation: glmS
sequence annotation: rhaB promoter
sequence annotation: sfGFP
sequence annotation: YebF
sequence annotation: rhaS
sequence annotation: Promoter 3BCD2
sequence annotation: L3S2P11 Terminator
sequence annotation: low TIR
sequence annotation: rhaR
sequence annotation: TcR
sequence annotation: pstS
sequence annotation: L3S3P22 Terminator (synthetic)
sequence annotation: rrnC
sequence: https://synbiohub.org/public/SEGA/SEGA053_seq/1

yebF
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
codon start: 1
db xref: ASAP:ABE-0006141
db xref: EcoGene:EG11807
db xref: GI:145698278
db xref: GeneID:946363
db xref: UniProtKB/Swiss-Prot:P33219
gene: yebF
gene synonym: ECK1848; JW1836
label: yebF
locus tag: b1847
note: yebFss
product: yebF signal sequence
protein id: NP_416361.2
translation: MKKRGAFLGLLLVSACASVFA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA053/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA053/annotation6/range/1

https://synbiohub.org/public/SEGA/SEGA053/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA053/1
start: 2656
end: 2718
orientation: inline

glmS
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
codon start: 1
label: glmS
note: glmS
translation: VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA053/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA053/annotation0/range/1

https://synbiohub.org/public/SEGA/SEGA053/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA053/1
start: 1
end: 528
orientation: inline

rhaB promoter
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
label: rhaB promoter
note: promoter of the E. coli rhaBAD operon, conferring tight induction with L-rhamnose and repression with D-glucose in the presence of RhaR and RhaS (Giacalone et al., 2006)
note: rhaB promoter
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA053/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA053/annotation4/range/1

https://synbiohub.org/public/SEGA/SEGA053/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA053/1
start: 2508
end: 2626
orientation: inline

sfGFP
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
codon start: 1
label: sfGFP
note: sfGFP
product: superfolder GFP
translation: RKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDGTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEFVTAAGITHGMDELYK
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA053/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA053/annotation8/range/1

https://synbiohub.org/public/SEGA/SEGA053/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA053/1
start: 3010
end: 3720
orientation: inline

YebF
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
codon start: 1
label: YebF
note: YebF
product: full size yebF protein
translation: ANNETSKSVTFPKCEDLDAAGIAASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHYQVSVDCKAGMAEYQRR
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA053/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA053/annotation7/range/1

https://synbiohub.org/public/SEGA/SEGA053/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA053/1
start: 2719
end: 3009
orientation: inline

rhaS
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
codon start: 1
gene: E. coli rhaS
label: rhaS
note: activated by L-rhamnose
note: rhaS
product: positive regulator of the rhaB promoter
translation: MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHGTGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLLPQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKSSLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQRYLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGFLQ
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA053/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA053/annotation3/range/1

https://synbiohub.org/public/SEGA/SEGA053/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA053/1
start: 1526
end: 2362
orientation: reverseComplement

Promoter 3BCD2
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
label: Promoter 3BCD2
note: Promoter 3BCD2
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA053/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA053/annotation10/range/1

https://synbiohub.org/public/SEGA/SEGA053/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA053/1
start: 3783
end: 3811
orientation: inline

L3S2P11 Terminator
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
label: L3S2P11 Terminator
note: L3S2P11 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA053/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA053/annotation1/range/1

https://synbiohub.org/public/SEGA/SEGA053/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA053/1
start: 538
end: 594
orientation: inline

low TIR
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
note: TIR
note: low TIR
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA053/1
role: SO:0000139
location: https://synbiohub.org/public/SEGA/SEGA053/annotation5/range/1

https://synbiohub.org/public/SEGA/SEGA053/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA053/1
start: 2638
end: 2664
orientation: inline

rhaR
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
codon start: 1
gene: E. coli rhaR
label: rhaR
note: needed for rhamnose-dependent activation of the rhaB promoter
note: rhaR
product: transcriptional activator of rhaR and rhaS
translation: MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQGAIPGFNASAGQPHWRLGSMGMAQARQVIGQLEHESSQHVPFANEMAELLFGQLVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRHLNSQKD
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA053/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA053/annotation2/range/1

https://synbiohub.org/public/SEGA/SEGA053/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA053/1
start: 607
end: 1452
orientation: reverseComplement

TcR
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
codon start: 1
gene: tet
label: TcR
note: TcR
note: confers resistance to tetracycline
product: tetracycline efflux protein
translation: MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHYGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVIFGEDRFRWSATMSGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA053/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA053/annotation11/range/1

https://synbiohub.org/public/SEGA/SEGA053/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA053/1
start: 3851
end: 5041
orientation: inline

pstS
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
codon start: 1
label: pstS
note: pstS
translation: MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKWADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA053/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA053/annotation13/range/1

https://synbiohub.org/public/SEGA/SEGA053/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA053/1
start: 5287
end: 5534
orientation: inline

L3S3P22 Terminator (synthetic)
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
label: L3S3P22 Terminator (synthetic)
note: L3S3P22 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA053/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA053/annotation9/range/1

https://synbiohub.org/public/SEGA/SEGA053/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA053/1
start: 3724
end: 3772
orientation: inline

rrnC
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
label: rrnC
note: rrnC
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA053/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA053/annotation12/range/1

https://synbiohub.org/public/SEGA/SEGA053/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA053/1
start: 5052
end: 5092
orientation: inline

https://synbiohub.org/public/SEGA/SEGA065/1
type: ComponentDefinition
persistent identity: SEGA065
display id: SEGA065
version: 1
description: natural linear DNA.
owned by: carbay
owned by: morno
date: 08-SEP-2021
division: UNA
keywords: .
locus: SEGA065
molecule: ds-DNA
organism: unspecified
reference: https://synbiohub.org/public/SEGA/reference0_1/1
source: natural DNA sequence
type: DnaRegion
type: SO:0000987
role: SO:0000804
sequence annotation: L3S2P11 Terminator
sequence annotation: T7 promoter
sequence annotation: Promoter 3BCD2
sequence annotation: L3S3P22 Terminator (synthetic)
sequence annotation: lac operator
sequence annotation: PROTi-Linker
sequence annotation: rrnC
sequence annotation: lacI
sequence annotation: TcR
sequence annotation: lacI promoter
sequence annotation: TEV site
sequence annotation: low TIR
sequence annotation: glmS
sequence annotation: pstS
sequence annotation: GFP
sequence: https://synbiohub.org/public/SEGA/SEGA065_seq/1

L3S2P11 Terminator
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
label: L3S2P11 Terminator
note: L3S2P11 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA065/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA065/annotation1/range/1

https://synbiohub.org/public/SEGA/SEGA065/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA065/1
start: 538
end: 594
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
label: T7 promoter
note: T7 promoter
note: promoter for bacteriophage T7 RNA polymerase
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA065/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA065/annotation4/range/1

https://synbiohub.org/public/SEGA/SEGA065/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA065/1
start: 1795
end: 1813
orientation: inline

Promoter 3BCD2
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
label: Promoter 3BCD2
note: Promoter 3BCD2
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA065/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA065/annotation11/range/1

https://synbiohub.org/public/SEGA/SEGA065/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA065/1
start: 2698
end: 2726
orientation: inline

L3S3P22 Terminator (synthetic)
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
label: L3S3P22 Terminator (synthetic)
note: L3S3P22 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA065/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA065/annotation10/range/1

https://synbiohub.org/public/SEGA/SEGA065/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA065/1
start: 2639
end: 2687
orientation: inline

lac operator
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
bound moiety: lac repressor encoded by lacI
feature type: protein_bind
label: lac operator
note: The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG).
note: lac operator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA065/1
role: SO:0000110
location: https://synbiohub.org/public/SEGA/SEGA065/annotation5/range/1

https://synbiohub.org/public/SEGA/SEGA065/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA065/1
start: 1814
end: 1838
orientation: inline

PROTi-Linker
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
label: PROTi-Linker
note: PROTi-Linker
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA065/1
role: SO:0001411
location: https://synbiohub.org/public/SEGA/SEGA065/annotation8/range/1

https://synbiohub.org/public/SEGA/SEGA065/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA065/1
start: 1889
end: 1924
orientation: inline

rrnC
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
label: rrnC
note: rrnC
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA065/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA065/annotation13/range/1

https://synbiohub.org/public/SEGA/SEGA065/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA065/1
start: 3967
end: 4007
orientation: inline

lacI
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
codon start: 1
gene: lacI
label: lacI
note: The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG).
note: lacI
product: lac repressor
translation: MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA065/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA065/annotation2/range/1

https://synbiohub.org/public/SEGA/SEGA065/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA065/1
start: 604
end: 1686
orientation: reverseComplement

TcR
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
codon start: 1
gene: tet
label: TcR
note: TcR
note: confers resistance to tetracycline
product: tetracycline efflux protein
translation: MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHYGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVIFGEDRFRWSATMSGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA065/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA065/annotation12/range/1

https://synbiohub.org/public/SEGA/SEGA065/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA065/1
start: 2766
end: 3956
orientation: inline

lacI promoter
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
gene: lacI
label: lacI promoter
note: lacI promoter
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA065/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA065/annotation3/range/1

https://synbiohub.org/public/SEGA/SEGA065/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA065/1
start: 1687
end: 1764
orientation: reverseComplement

TEV site
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
codon start: 1
label: TEV site
note: TEV site
product: tobacco etch virus (TEV) protease recognition and cleavage site
translation: MENLYFQ
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA065/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA065/annotation7/range/1

https://synbiohub.org/public/SEGA/SEGA065/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA065/1
start: 1868
end: 1888
orientation: inline

low TIR
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
note: TIR
note: low TIR
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA065/1
role: SO:0000139
location: https://synbiohub.org/public/SEGA/SEGA065/annotation6/range/1

https://synbiohub.org/public/SEGA/SEGA065/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA065/1
start: 1850
end: 1876
orientation: inline

glmS
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
codon start: 1
label: glmS
note: glmS
translation: VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA065/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA065/annotation0/range/1

https://synbiohub.org/public/SEGA/SEGA065/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA065/1
start: 1
end: 528
orientation: inline

pstS
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
codon start: 1
label: pstS
note: pstS
translation: MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKWADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA065/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA065/annotation14/range/1

https://synbiohub.org/public/SEGA/SEGA065/annotation14/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA065/1
start: 4202
end: 4449
orientation: inline

GFP
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
codon start: 1
label: GFP
note: GFP
product: green fluorescent protein
translation: SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYK
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA065/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA065/annotation9/range/1

https://synbiohub.org/public/SEGA/SEGA065/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA065/1
start: 1925
end: 2635
orientation: inline

https://synbiohub.org/public/SEGA/SEGA069/1
type: ComponentDefinition
persistent identity: SEGA069
display id: SEGA069
version: 1
description: natural linear DNA.
owned by: carbay
owned by: morno
date: 08-SEP-2021
division: UNA
keywords: .
locus: SEGA069
molecule: ds-DNA
organism: unspecified
reference: https://synbiohub.org/public/SEGA/reference0_1/1
source: natural DNA sequence
type: DnaRegion
type: SO:0000987
role: SO:0000804
sequence annotation: rrnC
sequence annotation: lacI
sequence annotation: L3S2P11 Terminator
sequence annotation: pelB signal sequence
sequence annotation: L3S3P22 Terminator (synthetic)
sequence annotation: glmS
sequence annotation: TcR
sequence annotation: pstS
sequence annotation: medium TIR
sequence annotation: lac operator
sequence annotation: T7 promoter
sequence annotation: sfGFP
sequence annotation: Promoter 3BCD2
sequence annotation: lacI promoter
sequence: https://synbiohub.org/public/SEGA/SEGA069_seq/1

rrnC
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
label: rrnC
note: rrnC
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA069/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA069/annotation12/range/1

https://synbiohub.org/public/SEGA/SEGA069/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA069/1
start: 3976
end: 4016
orientation: inline

lacI
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
codon start: 1
gene: lacI
label: lacI
note: The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG).
note: lacI
product: lac repressor
translation: MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA069/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA069/annotation2/range/1

https://synbiohub.org/public/SEGA/SEGA069/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA069/1
start: 604
end: 1686
orientation: reverseComplement

L3S2P11 Terminator
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
label: L3S2P11 Terminator
note: L3S2P11 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA069/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA069/annotation1/range/1

https://synbiohub.org/public/SEGA/SEGA069/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA069/1
start: 538
end: 594
orientation: inline

pelB signal sequence
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
codon start: 1
gene: pelB (fragment)
label: pelB signal sequence
note: pelB signal sequence
product: leader peptide for secretion
translation: MKYLLPTAAAGLLLLAAQPAMA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA069/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA069/annotation7/range/1

https://synbiohub.org/public/SEGA/SEGA069/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA069/1
start: 1868
end: 1933
orientation: inline

L3S3P22 Terminator (synthetic)
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
label: L3S3P22 Terminator (synthetic)
note: L3S3P22 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA069/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA069/annotation9/range/1

https://synbiohub.org/public/SEGA/SEGA069/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA069/1
start: 2648
end: 2696
orientation: inline

glmS
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
codon start: 1
label: glmS
note: glmS
translation: VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA069/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA069/annotation0/range/1

https://synbiohub.org/public/SEGA/SEGA069/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA069/1
start: 1
end: 528
orientation: inline

TcR
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
codon start: 1
gene: tet
label: TcR
note: TcR
note: confers resistance to tetracycline
product: tetracycline efflux protein
translation: MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHYGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVIFGEDRFRWSATMSGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA069/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA069/annotation11/range/1

https://synbiohub.org/public/SEGA/SEGA069/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA069/1
start: 2775
end: 3965
orientation: inline

pstS
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
codon start: 1
label: pstS
note: pstS
translation: MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKWADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA069/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA069/annotation13/range/1

https://synbiohub.org/public/SEGA/SEGA069/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA069/1
start: 4211
end: 4458
orientation: inline

medium TIR
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
note: TIR
note: medium TIR
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA069/1
role: SO:0000139
location: https://synbiohub.org/public/SEGA/SEGA069/annotation6/range/1

https://synbiohub.org/public/SEGA/SEGA069/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA069/1
start: 1850
end: 1876
orientation: inline

lac operator
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
bound moiety: lac repressor encoded by lacI
feature type: protein_bind
label: lac operator
note: The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG).
note: lac operator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA069/1
role: SO:0000110
location: https://synbiohub.org/public/SEGA/SEGA069/annotation5/range/1

https://synbiohub.org/public/SEGA/SEGA069/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA069/1
start: 1814
end: 1838
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
label: T7 promoter
note: T7 promoter
note: promoter for bacteriophage T7 RNA polymerase
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA069/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA069/annotation4/range/1

https://synbiohub.org/public/SEGA/SEGA069/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA069/1
start: 1795
end: 1813
orientation: inline

sfGFP
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
codon start: 1
label: sfGFP
note: sfGFP
product: superfolder GFP
translation: RKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDGTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEFVTAAGITHGMDELYK
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA069/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA069/annotation8/range/1

https://synbiohub.org/public/SEGA/SEGA069/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA069/1
start: 1934
end: 2644
orientation: inline

Promoter 3BCD2
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
label: Promoter 3BCD2
note: Promoter 3BCD2
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA069/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA069/annotation10/range/1

https://synbiohub.org/public/SEGA/SEGA069/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA069/1
start: 2707
end: 2735
orientation: inline

lacI promoter
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
gene: lacI
label: lacI promoter
note: lacI promoter
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA069/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA069/annotation3/range/1

https://synbiohub.org/public/SEGA/SEGA069/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA069/1
start: 1687
end: 1764
orientation: reverseComplement

https://synbiohub.org/public/SEGA/SEGA037/1
type: ComponentDefinition
persistent identity: SEGA037
display id: SEGA037
version: 1
description: natural linear DNA.
owned by: carbay
owned by: morno
date: 09-SEP-2021
division: UNA
keywords: .
locus: SEGA037
molecule: ds-DNA
organism: unspecified
reference: https://synbiohub.org/user/carbay/SEGA/SEGA037_reference0/1
source: natural DNA sequence
type: DnaRegion
type: SO:0000987
role: SO:0000804
sequence annotation: L3S2P11 Terminator
sequence annotation: glmS
sequence annotation: YebFss
sequence annotation: TcR
sequence annotation: lacO
sequence annotation: high TIR
sequence annotation: lacI
sequence annotation: Promoter 3BCD2
sequence annotation: YebF
sequence annotation: lacI promoter
sequence annotation: L3S3P22 Terminator (synthetic)
sequence annotation: rrnC
sequence annotation: pstS
sequence annotation: sfGFP
sequence annotation: trc promoter
sequence: https://synbiohub.org/public/SEGA/SEGA037_seq/1

L3S2P11 Terminator
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
label: L3S2P11 Terminator
note: L3S2P11 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA037/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA037/annotation1/range/1

https://synbiohub.org/public/SEGA/SEGA037/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA037/1
start: 538
end: 594
orientation: inline

glmS
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
codon start: 1
label: glmS
note: glmS
translation: VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA037/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA037/annotation0/range/1

https://synbiohub.org/public/SEGA/SEGA037/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA037/1
start: 1
end: 528
orientation: inline

YebFss
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
codon start: 1
db xref: ASAP:ABE-0006141
db xref: EcoGene:EG11807
db xref: GI:145698278
db xref: GeneID:946363
db xref: UniProtKB/Swiss-Prot:P33219
gene: yebF
gene synonym: ECK1848; JW1836
label: YebFss
locus tag: b1847
note: YebFss
product: yebF signal sequence
protein id: NP_416361.2
translation: MKKRGAFLGLLLVSACASVFA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA037/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA037/annotation7/range/1

https://synbiohub.org/public/SEGA/SEGA037/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA037/1
start: 1878
end: 1940
orientation: inline

TcR
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
codon start: 1
gene: tet
label: TcR
note: TcR
note: confers resistance to tetracycline
product: tetracycline efflux protein
translation: MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHYGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVIFGEDRFRWSATMSGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA037/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA037/annotation12/range/1

https://synbiohub.org/public/SEGA/SEGA037/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA037/1
start: 3073
end: 4263
orientation: inline

lacO
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
bound moiety: lac repressor
feature type: protein_bind
label: lacO
note: lac operator
note: lacO
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA037/1
role: SO:0000110
location: https://synbiohub.org/public/SEGA/SEGA037/annotation5/range/1

https://synbiohub.org/public/SEGA/SEGA037/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA037/1
start: 1825
end: 1848
orientation: inline

high TIR
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
note: TIR
note: high TIR
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA037/1
role: SO:0000139
location: https://synbiohub.org/public/SEGA/SEGA037/annotation6/range/1

https://synbiohub.org/public/SEGA/SEGA037/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA037/1
start: 1860
end: 1886
orientation: inline

lacI
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
codon start: 1
gene: lacI
label: lacI
note: The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG).
note: lacI
product: lac repressor
translation: MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA037/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA037/annotation2/range/1

https://synbiohub.org/public/SEGA/SEGA037/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA037/1
start: 604
end: 1686
orientation: reverseComplement

Promoter 3BCD2
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
label: Promoter 3BCD2
note: Promoter 3BCD2
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA037/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA037/annotation11/range/1

https://synbiohub.org/public/SEGA/SEGA037/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA037/1
start: 3005
end: 3033
orientation: inline

YebF
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
codon start: 1
label: YebF
note: YebF
product: full size yebF protein
translation: ANNETSKSVTFPKCEDLDAAGIAASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHYQVSVDCKAGMAEYQRR
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA037/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA037/annotation8/range/1

https://synbiohub.org/public/SEGA/SEGA037/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA037/1
start: 1941
end: 2231
orientation: inline

lacI promoter
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
gene: lacI
label: lacI promoter
note: lacI promoter
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA037/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA037/annotation3/range/1

https://synbiohub.org/public/SEGA/SEGA037/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA037/1
start: 1687
end: 1764
orientation: reverseComplement

L3S3P22 Terminator (synthetic)
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
label: L3S3P22 Terminator (synthetic)
note: L3S3P22 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA037/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA037/annotation10/range/1

https://synbiohub.org/public/SEGA/SEGA037/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA037/1
start: 2946
end: 2994
orientation: inline

rrnC
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
label: rrnC
note: rrnC
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA037/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA037/annotation13/range/1

https://synbiohub.org/public/SEGA/SEGA037/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA037/1
start: 4274
end: 4314
orientation: inline

pstS
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
codon start: 1
label: pstS
note: pstS
translation: MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKWADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA037/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA037/annotation14/range/1

https://synbiohub.org/public/SEGA/SEGA037/annotation14/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA037/1
start: 4509
end: 4756
orientation: inline

sfGFP
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
codon start: 1
label: sfGFP
note: sfGFP
product: superfolder GFP
translation: RKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDGTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEFVTAAGITHGMDELYK
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA037/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA037/annotation9/range/1

https://synbiohub.org/public/SEGA/SEGA037/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA037/1
start: 2232
end: 2942
orientation: inline

trc promoter
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
label: trc promoter
note: strong E. coli promoter; hybrid between the trp and lac UV5 promoters
note: trc promoter
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA037/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA037/annotation4/range/1

https://synbiohub.org/public/SEGA/SEGA037/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA037/1
start: 1795
end: 1824
orientation: inline

https://synbiohub.org/public/SEGA/SEGA009/1
type: ComponentDefinition
persistent identity: SEGA009
display id: SEGA009
version: 1
description: natural linear DNA.
owned by: carbay
owned by: morno
date: 09-SEP-2021
division: UNA
keywords: .
locus: SEGA009
molecule: ds-DNA
organism: unspecified
reference: https://synbiohub.org/public/SEGA/reference0_1/1
source: natural DNA sequence
type: DnaRegion
type: SO:0000987
role: SO:0000804
sequence annotation: Promoter 3BCD2
sequence annotation: pstS
sequence annotation: glmS
sequence annotation: medium TIR
sequence annotation: Ubiquitin
sequence annotation: L3S3P22 Terminator (synthetic)
sequence annotation: GFP
sequence annotation: P-J23100
sequence annotation: L3S2P11 Terminator
sequence annotation: TcR
sequence annotation: rrnC
sequence: https://synbiohub.org/public/SEGA/SEGA009_seq/1

Promoter 3BCD2
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
label: Promoter 3BCD2
note: Promoter 3BCD2
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA009/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA009/annotation7/range/1

https://synbiohub.org/public/SEGA/SEGA009/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA009/1
start: 1750
end: 1778
orientation: inline

pstS
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
codon start: 1
label: pstS
note: pstS
translation: MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKWADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA009/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA009/annotation10/range/1

https://synbiohub.org/public/SEGA/SEGA009/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA009/1
start: 3254
end: 3501
orientation: inline

glmS
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
codon start: 1
label: glmS
note: glmS
translation: VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA009/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA009/annotation0/range/1

https://synbiohub.org/public/SEGA/SEGA009/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA009/1
start: 1
end: 528
orientation: inline

medium TIR
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
note: TIR
note: medium TIR
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA009/1
role: SO:0000139
location: https://synbiohub.org/public/SEGA/SEGA009/annotation3/range/1

https://synbiohub.org/public/SEGA/SEGA009/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA009/1
start: 650
end: 676
orientation: inline

Ubiquitin
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
codon start: 1
label: Ubiquitin
note: Ubiquitin
translation: MQIFVKTLTGKTITLEVEPGSAHHHHHHHHHHAGSSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQEKSTLELVLELQNETQSATSDA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA009/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA009/annotation4/range/1

https://synbiohub.org/public/SEGA/SEGA009/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA009/1
start: 668
end: 967
orientation: inline

L3S3P22 Terminator (synthetic)
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
label: L3S3P22 Terminator (synthetic)
note: L3S3P22 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA009/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA009/annotation6/range/1

https://synbiohub.org/public/SEGA/SEGA009/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA009/1
start: 1691
end: 1739
orientation: inline

GFP
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
codon start: 1
label: GFP
note: GFP
product: green fluorescent protein
translation: SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYK
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA009/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA009/annotation5/range/1

https://synbiohub.org/public/SEGA/SEGA009/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA009/1
start: 977
end: 1687
orientation: inline

P-J23100
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
label: P-J23100
note: P-J23100
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA009/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA009/annotation2/range/1

https://synbiohub.org/public/SEGA/SEGA009/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA009/1
start: 604
end: 638
orientation: inline

L3S2P11 Terminator
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
label: L3S2P11 Terminator
note: L3S2P11 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA009/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA009/annotation1/range/1

https://synbiohub.org/public/SEGA/SEGA009/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA009/1
start: 538
end: 594
orientation: inline

TcR
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
codon start: 1
gene: tet
label: TcR
note: TcR
note: confers resistance to tetracycline
product: tetracycline efflux protein
translation: MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHYGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVIFGEDRFRWSATMSGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA009/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA009/annotation8/range/1

https://synbiohub.org/public/SEGA/SEGA009/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA009/1
start: 1818
end: 3008
orientation: inline

rrnC
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
label: rrnC
note: rrnC
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA009/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA009/annotation9/range/1

https://synbiohub.org/public/SEGA/SEGA009/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA009/1
start: 3019
end: 3059
orientation: inline

https://synbiohub.org/public/SEGA/SEGA056/1
type: ComponentDefinition
persistent identity: SEGA056
display id: SEGA056
version: 1
description: natural linear DNA.
owned by: carbay
owned by: morno
date: 08-SEP-2021
division: UNA
keywords: .
locus: SEGA056
molecule: ds-DNA
organism: unspecified
reference: https://synbiohub.org/user/carbay/SEGA/SEGA056_reference0/1
source: natural DNA sequence
type: DnaRegion
type: SO:0000987
role: SO:0000804
sequence annotation: low TIR
sequence annotation: GFP
sequence annotation: Promoter 3BCD2
sequence annotation: L3S3P22 Terminator (synthetic)
sequence annotation: TcR
sequence annotation: lacI
sequence annotation: lacI promoter
sequence annotation: BCD
sequence annotation: lac operator
sequence annotation: rrnC
sequence annotation: glmS
sequence annotation: L3S2P11 Terminator
sequence annotation: pstS
sequence annotation: T7 promoter
sequence: https://synbiohub.org/public/SEGA/SEGA056_seq/1

low TIR
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
note: TIR
note: low TIR
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA056/1
role: SO:0000139
location: https://synbiohub.org/public/SEGA/SEGA056/annotation6/range/1

https://synbiohub.org/public/SEGA/SEGA056/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA056/1
start: 1850
end: 1876
orientation: inline

GFP
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
codon start: 1
label: GFP
note: GFP
product: green fluorescent protein
translation: SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYK
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA056/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA056/annotation8/range/1

https://synbiohub.org/public/SEGA/SEGA056/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA056/1
start: 1924
end: 2634
orientation: inline

Promoter 3BCD2
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
label: Promoter 3BCD2
note: Promoter 3BCD2
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA056/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA056/annotation10/range/1

https://synbiohub.org/public/SEGA/SEGA056/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA056/1
start: 2697
end: 2725
orientation: inline

L3S3P22 Terminator (synthetic)
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
label: L3S3P22 Terminator (synthetic)
note: L3S3P22 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA056/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA056/annotation9/range/1

https://synbiohub.org/public/SEGA/SEGA056/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA056/1
start: 2638
end: 2686
orientation: inline

TcR
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
codon start: 1
gene: tet
label: TcR
note: TcR
note: confers resistance to tetracycline
product: tetracycline efflux protein
translation: MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHYGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVIFGEDRFRWSATMSGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA056/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA056/annotation11/range/1

https://synbiohub.org/public/SEGA/SEGA056/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA056/1
start: 2765
end: 3955
orientation: inline

lacI
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
codon start: 1
gene: lacI
label: lacI
note: The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG).
note: lacI
product: lac repressor
translation: MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA056/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA056/annotation2/range/1

https://synbiohub.org/public/SEGA/SEGA056/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA056/1
start: 604
end: 1686
orientation: reverseComplement

lacI promoter
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
gene: lacI
label: lacI promoter
note: lacI promoter
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA056/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA056/annotation3/range/1

https://synbiohub.org/public/SEGA/SEGA056/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA056/1
start: 1687
end: 1764
orientation: reverseComplement

BCD
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
codon start: 1
label: BCD
note: BCD
translation: MKAIFVLKHLNHAKEVF
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA056/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA056/annotation7/range/1

https://synbiohub.org/public/SEGA/SEGA056/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA056/1
start: 1868
end: 1923
orientation: inline

lac operator
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
bound moiety: lac repressor encoded by lacI
feature type: protein_bind
label: lac operator
note: The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG).
note: lac operator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA056/1
role: SO:0000110
location: https://synbiohub.org/public/SEGA/SEGA056/annotation5/range/1

https://synbiohub.org/public/SEGA/SEGA056/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA056/1
start: 1814
end: 1838
orientation: inline

rrnC
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
label: rrnC
note: rrnC
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA056/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA056/annotation12/range/1

https://synbiohub.org/public/SEGA/SEGA056/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA056/1
start: 3966
end: 4006
orientation: inline

glmS
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
codon start: 1
label: glmS
note: glmS
translation: VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA056/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA056/annotation0/range/1

https://synbiohub.org/public/SEGA/SEGA056/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA056/1
start: 1
end: 528
orientation: inline

L3S2P11 Terminator
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
label: L3S2P11 Terminator
note: L3S2P11 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA056/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA056/annotation1/range/1

https://synbiohub.org/public/SEGA/SEGA056/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA056/1
start: 538
end: 594
orientation: inline

pstS
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
codon start: 1
label: pstS
note: pstS
translation: MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKWADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA056/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA056/annotation13/range/1

https://synbiohub.org/public/SEGA/SEGA056/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA056/1
start: 4201
end: 4448
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
label: T7 promoter
note: T7 promoter
note: promoter for bacteriophage T7 RNA polymerase
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA056/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA056/annotation4/range/1

https://synbiohub.org/public/SEGA/SEGA056/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA056/1
start: 1795
end: 1813
orientation: inline

https://synbiohub.org/public/SEGA/SEGA074/1
type: ComponentDefinition
persistent identity: SEGA074
display id: SEGA074
version: 1
description: natural linear DNA.
owned by: carbay
owned by: morno
date: 09-SEP-2021
division: UNA
keywords: .
locus: SEGA074
molecule: ds-DNA
organism: unspecified
reference: https://synbiohub.org/user/carbay/SEGA/SEGA074_reference0/1
source: natural DNA sequence
type: DnaRegion
type: SO:0000987
role: SO:0000804
sequence annotation: L3S3P22 Terminator (synthetic)
sequence annotation: RiboJ
sequence annotation: NahR-AM
sequence annotation: BCD
sequence annotation: Promoter 3BCD2
sequence annotation: rrnC
sequence annotation: low TIR
sequence annotation: L3S2P11 Terminator
sequence annotation: glmS
sequence annotation: TcR
sequence annotation: nah2
sequence annotation: pstS
sequence annotation: GFP
sequence annotation: pLacIQ
sequence annotation: pSalTTC
sequence: https://synbiohub.org/public/SEGA/SEGA074_seq/1

L3S3P22 Terminator (synthetic)
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
label: L3S3P22 Terminator (synthetic)
note: L3S3P22 Terminator (synthetic)
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA074/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA074/annotation10/range/1

https://synbiohub.org/public/SEGA/SEGA074/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA074/1
start: 2613
end: 2661
orientation: inline

RiboJ
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
label: RiboJ
note: RiboJ
note: insulator: self-cleaving ribozyme RiboJ
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA074/1
role: SO:0001411
location: https://synbiohub.org/public/SEGA/SEGA074/annotation6/range/1

https://synbiohub.org/public/SEGA/SEGA074/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA074/1
start: 1739
end: 1813
orientation: inline

NahR-AM
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
label: NahR-AM
note: NahR-AM
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA074/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA074/annotation2/range/1

https://synbiohub.org/public/SEGA/SEGA074/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA074/1
start: 611
end: 1513
orientation: reverseComplement

BCD
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
codon start: 1
label: BCD
note: BCD
translation: MKAIFVLKHLNHAKEVF
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA074/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA074/annotation8/range/1

https://synbiohub.org/public/SEGA/SEGA074/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA074/1
start: 1843
end: 1898
orientation: inline

Promoter 3BCD2
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
label: Promoter 3BCD2
note: Promoter 3BCD2
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA074/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA074/annotation11/range/1

https://synbiohub.org/public/SEGA/SEGA074/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA074/1
start: 2672
end: 2700
orientation: inline

rrnC
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
label: rrnC
note: rrnC
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA074/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA074/annotation13/range/1

https://synbiohub.org/public/SEGA/SEGA074/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA074/1
start: 3941
end: 3981
orientation: inline

low TIR
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
note: TIR
note: low TIR
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA074/1
role: SO:0000139
location: https://synbiohub.org/public/SEGA/SEGA074/annotation7/range/1

https://synbiohub.org/public/SEGA/SEGA074/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA074/1
start: 1825
end: 1851
orientation: inline

L3S2P11 Terminator
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
label: L3S2P11 Terminator
note: L3S2P11 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA074/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA074/annotation1/range/1

https://synbiohub.org/public/SEGA/SEGA074/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA074/1
start: 538
end: 594
orientation: inline

glmS
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
codon start: 1
label: glmS
note: glmS
translation: VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA074/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA074/annotation0/range/1

https://synbiohub.org/public/SEGA/SEGA074/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA074/1
start: 1
end: 528
orientation: inline

TcR
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
codon start: 1
gene: tet
label: TcR
note: TcR
note: confers resistance to tetracycline
product: tetracycline efflux protein
translation: MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHYGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVIFGEDRFRWSATMSGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA074/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA074/annotation12/range/1

https://synbiohub.org/public/SEGA/SEGA074/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA074/1
start: 2740
end: 3930
orientation: inline

nah2
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
label: nah2
note: nah2
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA074/1
role: SO:0000139
location: https://synbiohub.org/public/SEGA/SEGA074/annotation3/range/1

https://synbiohub.org/public/SEGA/SEGA074/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA074/1
start: 1514
end: 1542
orientation: inline

pstS
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
codon start: 1
label: pstS
note: pstS
translation: MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKWADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA074/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA074/annotation14/range/1

https://synbiohub.org/public/SEGA/SEGA074/annotation14/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA074/1
start: 4176
end: 4423
orientation: inline

GFP
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
codon start: 1
label: GFP
note: GFP
product: green fluorescent protein
translation: SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYK
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA074/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA074/annotation9/range/1

https://synbiohub.org/public/SEGA/SEGA074/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA074/1
start: 1899
end: 2609
orientation: inline

pLacIQ
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
label: pLacIQ
note: lacI promoter
note: pLacIQ
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA074/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA074/annotation4/range/1

https://synbiohub.org/public/SEGA/SEGA074/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA074/1
start: 1543
end: 1596
orientation: reverseComplement

pSalTTC
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
label: pSalTTC
note: pSalTTC
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA074/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA074/annotation5/range/1

https://synbiohub.org/public/SEGA/SEGA074/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA074/1
start: 1623
end: 1738
orientation: inline

https://synbiohub.org/public/SEGA/SEGA006/1
type: ComponentDefinition
persistent identity: SEGA006
display id: SEGA006
version: 1
description: natural linear DNA.
owned by: carbay
owned by: morno
date: 09-SEP-2021
division: UNA
keywords: .
locus: SEGA006
molecule: ds-DNA
organism: unspecified
reference: https://synbiohub.org/user/carbay/SEGA/SEGA006_reference0/1
source: natural DNA sequence
type: DnaRegion
type: SO:0000987
role: SO:0000804
sequence annotation: P-J23100
sequence annotation: L3S3P22 Terminator (synthetic)
sequence annotation: L3S2P11 Terminator
sequence annotation: rrnC
sequence annotation: Promoter 3BCD2
sequence annotation: pstS
sequence annotation: medium TIR
sequence annotation: TcR
sequence annotation: glmS
sequence annotation: BCD
sequence annotation: GFP
sequence: https://synbiohub.org/public/SEGA/SEGA006_seq/1

P-J23100
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
label: P-J23100
note: P-J23100
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA006/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA006/annotation2/range/1

https://synbiohub.org/public/SEGA/SEGA006/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA006/1
start: 604
end: 638
orientation: inline

L3S3P22 Terminator (synthetic)
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
label: L3S3P22 Terminator (synthetic)
note: L3S3P22 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA006/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA006/annotation6/range/1

https://synbiohub.org/public/SEGA/SEGA006/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA006/1
start: 1438
end: 1486
orientation: inline

L3S2P11 Terminator
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
label: L3S2P11 Terminator
note: L3S2P11 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA006/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA006/annotation1/range/1

https://synbiohub.org/public/SEGA/SEGA006/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA006/1
start: 538
end: 594
orientation: inline

rrnC
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
label: rrnC
note: rrnC
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA006/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA006/annotation9/range/1

https://synbiohub.org/public/SEGA/SEGA006/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA006/1
start: 2766
end: 2806
orientation: inline

Promoter 3BCD2
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
label: Promoter 3BCD2
note: Promoter 3BCD2
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA006/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA006/annotation7/range/1

https://synbiohub.org/public/SEGA/SEGA006/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA006/1
start: 1497
end: 1525
orientation: inline

pstS
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
codon start: 1
label: pstS
note: pstS
translation: MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKWADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA006/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA006/annotation10/range/1

https://synbiohub.org/public/SEGA/SEGA006/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA006/1
start: 3001
end: 3248
orientation: inline

medium TIR
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
note: TIR
note: medium TIR
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA006/1
role: SO:0000139
location: https://synbiohub.org/public/SEGA/SEGA006/annotation3/range/1

https://synbiohub.org/public/SEGA/SEGA006/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA006/1
start: 650
end: 676
orientation: inline

TcR
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
codon start: 1
gene: tet
label: TcR
note: TcR
note: confers resistance to tetracycline
product: tetracycline efflux protein
translation: MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHYGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVIFGEDRFRWSATMSGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA006/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA006/annotation8/range/1

https://synbiohub.org/public/SEGA/SEGA006/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA006/1
start: 1565
end: 2755
orientation: inline

glmS
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
codon start: 1
label: glmS
note: glmS
translation: VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA006/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA006/annotation0/range/1

https://synbiohub.org/public/SEGA/SEGA006/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA006/1
start: 1
end: 528
orientation: inline

BCD
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
codon start: 1
label: BCD
note: BCD
translation: MKAIFVLKHLNHAKEVF
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA006/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA006/annotation4/range/1

https://synbiohub.org/public/SEGA/SEGA006/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA006/1
start: 668
end: 723
orientation: inline

GFP
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
codon start: 1
label: GFP
note: GFP
product: green fluorescent protein
translation: SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYK
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA006/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA006/annotation5/range/1

https://synbiohub.org/public/SEGA/SEGA006/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA006/1
start: 724
end: 1434
orientation: inline

https://synbiohub.org/public/SEGA/SEGA097/1
type: ComponentDefinition
persistent identity: SEGA097
display id: SEGA097
version: 1
description: natural linear DNA.
owned by: carbay
owned by: morno
date: 09-SEP-2021
division: UNA
keywords: .
locus: SEGA097
molecule: ds-DNA
organism: unspecified
reference: https://synbiohub.org/user/carbay/SEGA/SEGA097_reference0/1
source: natural DNA sequence
type: DnaRegion
type: SO:0000987
role: SO:0000804
sequence annotation: rrnC
sequence annotation: high TIR
sequence annotation: L3S2P11 Terminator
sequence annotation: glmS
sequence annotation: P-J23100
sequence annotation: TcR
sequence annotation: pstS
sequence annotation: BCD
sequence: https://synbiohub.org/public/SEGA/SEGA097_seq/1

rrnC
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
label: rrnC
note: rrnC
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA097/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA097/annotation6/range/1

https://synbiohub.org/public/SEGA/SEGA097/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA097/1
start: 1847
end: 1887
orientation: inline

high TIR
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
note: TIR
note: high TIR
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA097/1
role: SO:0000139
location: https://synbiohub.org/public/SEGA/SEGA097/annotation3/range/1

https://synbiohub.org/public/SEGA/SEGA097/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA097/1
start: 650
end: 676
orientation: inline

L3S2P11 Terminator
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
label: L3S2P11 Terminator
note: L3S2P11 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA097/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA097/annotation1/range/1

https://synbiohub.org/public/SEGA/SEGA097/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA097/1
start: 538
end: 594
orientation: inline

glmS
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
codon start: 1
label: glmS
note: glmS
translation: VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA097/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA097/annotation0/range/1

https://synbiohub.org/public/SEGA/SEGA097/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA097/1
start: 1
end: 528
orientation: inline

P-J23100
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
label: P-J23100
note: P-J23100
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA097/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA097/annotation2/range/1

https://synbiohub.org/public/SEGA/SEGA097/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA097/1
start: 604
end: 638
orientation: inline

TcR
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
codon start: 1
gene: tet
label: TcR
note: TcR
note: confers resistance to tetracycline
product: tetracycline efflux protein
translation: VLPGLLRDIVHSDSIASHYGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVIFGEDRFRWSATMSGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA097/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA097/annotation5/range/1

https://synbiohub.org/public/SEGA/SEGA097/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA097/1
start: 724
end: 1836
orientation: inline

pstS
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
codon start: 1
label: pstS
note: pstS
translation: MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKWADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA097/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA097/annotation7/range/1

https://synbiohub.org/public/SEGA/SEGA097/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA097/1
start: 2082
end: 2329
orientation: inline

BCD
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
codon start: 1
label: BCD
note: BCD
translation: MKAIFVLKHLNHAKEVF
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA097/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA097/annotation4/range/1

https://synbiohub.org/public/SEGA/SEGA097/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA097/1
start: 668
end: 723
orientation: inline

https://synbiohub.org/public/SEGA/SEGA090/1
type: ComponentDefinition
persistent identity: SEGA090
display id: SEGA090
version: 1
description: natural linear DNA.
owned by: carbay
owned by: morno
date: 09-SEP-2021
division: UNA
keywords: .
locus: SEGA090
molecule: ds-DNA
organism: unspecified
reference: https://synbiohub.org/user/carbay/SEGA/SEGA090_reference0/1
source: natural DNA sequence
type: DnaRegion
type: SO:0000987
role: SO:0000804
sequence annotation: glmS
sequence annotation: L3S3P22 Terminator (synthetic)
sequence annotation: rrnC
sequence annotation: pelB signal sequence
sequence annotation: Promoter 3BCD2
sequence annotation: TcR
sequence annotation: EM7 promoter
sequence annotation: rhaS
sequence annotation: L3S2P11 Terminator
sequence annotation: rhaR
sequence annotation: low TIR
sequence annotation: rhaB promoter
sequence annotation: pstS
sequence annotation: Terminator rrnB T1
sequence annotation: sfGFP
sequence annotation: galK
sequence: https://synbiohub.org/public/SEGA/SEGA090_seq/1

glmS
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
codon start: 1
label: glmS
note: glmS
translation: VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA090/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA090/annotation0/range/1

https://synbiohub.org/public/SEGA/SEGA090/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA090/1
start: 1
end: 528
orientation: inline

L3S3P22 Terminator (synthetic)
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
label: L3S3P22 Terminator (synthetic)
note: L3S3P22 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA090/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA090/annotation11/range/1

https://synbiohub.org/public/SEGA/SEGA090/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA090/1
start: 4728
end: 4776
orientation: inline

rrnC
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
label: rrnC
note: rrnC
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA090/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA090/annotation14/range/1

https://synbiohub.org/public/SEGA/SEGA090/annotation14/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA090/1
start: 6056
end: 6096
orientation: inline

pelB signal sequence
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
codon start: 1
gene: pelB (fragment)
label: pelB signal sequence
note: pelB signal sequence
product: leader peptide for secretion
translation: MKYLLPTAAAGLLLLAAQPAMA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA090/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA090/annotation9/range/1

https://synbiohub.org/public/SEGA/SEGA090/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA090/1
start: 3948
end: 4013
orientation: inline

Promoter 3BCD2
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
label: Promoter 3BCD2
note: Promoter 3BCD2
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA090/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA090/annotation12/range/1

https://synbiohub.org/public/SEGA/SEGA090/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA090/1
start: 4787
end: 4815
orientation: inline

TcR
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
codon start: 1
gene: tet
label: TcR
note: TcR
note: confers resistance to tetracycline
product: tetracycline efflux protein
translation: MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHYGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA090/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA090/annotation13/range/1

https://synbiohub.org/public/SEGA/SEGA090/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA090/1
start: 4855
end: 6045
orientation: inline

EM7 promoter
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
label: EM7 promoter
note: EM7 promoter
note: synthetic bacterial promoter
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA090/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA090/annotation3/range/1

https://synbiohub.org/public/SEGA/SEGA090/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA090/1
start: 1783
end: 1830
orientation: reverseComplement

rhaS
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
codon start: 1
gene: E. coli rhaS
label: rhaS
note: activated by L-rhamnose
note: rhaS
product: positive regulator of the rhaB promoter
translation: MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHGTGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLLPQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKSSLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQRYLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGFLQ
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA090/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA090/annotation6/range/1

https://synbiohub.org/public/SEGA/SEGA090/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA090/1
start: 2818
end: 3654
orientation: reverseComplement

L3S2P11 Terminator
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
label: L3S2P11 Terminator
note: L3S2P11 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA090/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA090/annotation1/range/1

https://synbiohub.org/public/SEGA/SEGA090/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA090/1
start: 538
end: 594
orientation: inline

rhaR
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
codon start: 1
gene: E. coli rhaR
label: rhaR
note: needed for rhamnose-dependent activation of the rhaB promoter
note: rhaR
product: transcriptional activator of rhaR and rhaS
translation: MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQGAIPGFNASAGQPHWRLGSMGMAQARQVIGQLEHESSQHVPFANEMAELLFGQLVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRHLNSQKD
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA090/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA090/annotation5/range/1

https://synbiohub.org/public/SEGA/SEGA090/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA090/1
start: 1899
end: 2744
orientation: reverseComplement

low TIR
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
note: TIR
note: low TIR
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA090/1
role: SO:0000173
location: https://synbiohub.org/public/SEGA/SEGA090/annotation8/range/1

https://synbiohub.org/public/SEGA/SEGA090/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA090/1
start: 3930
end: 3956
orientation: inline

rhaB promoter
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
label: rhaB promoter
note: promoter of the E. coli rhaBAD operon, conferring tight induction with L-rhamnose and repression with D-glucose in the presence of RhaR and RhaS (Giacalone et al., 2006)
note: rhaB promoter
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA090/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA090/annotation7/range/1

https://synbiohub.org/public/SEGA/SEGA090/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA090/1
start: 3800
end: 3918
orientation: inline

pstS
type: SequenceAnnotation
persistent identity: annotation15
display id: annotation15
version: 1
codon start: 1
label: pstS
note: pstS
translation: MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKWADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA090/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA090/annotation15/range/1

https://synbiohub.org/public/SEGA/SEGA090/annotation15/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA090/1
start: 6291
end: 6538
orientation: inline

Terminator rrnB T1
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
label: Terminator rrnB T1
note: Terminator rrnB T1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA090/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA090/annotation4/range/1

https://synbiohub.org/public/SEGA/SEGA090/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA090/1
start: 1843
end: 1886
orientation: inline

sfGFP
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
codon start: 1
label: sfGFP
note: sfGFP
product: superfolder GFP
translation: RKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDGTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEFVTAAGITHGMDELYK
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA090/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA090/annotation10/range/1

https://synbiohub.org/public/SEGA/SEGA090/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA090/1
start: 4014
end: 4724
orientation: inline

galK
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
EC number: 2.7.1.6
codon start: 1
db xref: ASAP:ABE-0002568
db xref: EcoGene:EG10363
db xref: GI:16128725
db xref: GeneID:945358
db xref: UniProtKB/Swiss-Prot:P0A6T3
function: enzyme; Degradation of small molecules: Carbon compounds
gene: galK
gene synonym: ECK0746; galA; JW0740
label: galK
locus tag: b0757
note: galK
product: galactokinase
protein id: NP_415278.1
translation: MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVLPCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLDAPIVAHENYQWANYVRGVVKHLQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQEAENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTLVGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTVEAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGGGFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA090/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA090/annotation2/range/1

https://synbiohub.org/public/SEGA/SEGA090/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA090/1
start: 605
end: 1753
orientation: reverseComplement

https://synbiohub.org/public/SEGA/SEGA101/1
type: ComponentDefinition
persistent identity: SEGA101
display id: SEGA101
version: 1
description: natural linear DNA.
owned by: carbay
owned by: morno
date: 09-SEP-2021
division: UNA
keywords: .
locus: SEGA101
molecule: ds-DNA
organism: unspecified
reference: https://synbiohub.org/user/carbay/SEGA/SEGA101_reference0/1
source: natural DNA sequence
type: DnaRegion
type: SO:0000987
role: SO:0000804
sequence annotation: rrnC
sequence annotation: L3S2P11 Terminator
sequence annotation: glmS
sequence annotation: TcR
sequence annotation: rhaS
sequence annotation: rhaR
sequence annotation: pstS
sequence annotation: BCD
sequence annotation: rhaB promoter
sequence annotation: low TIR
sequence: https://synbiohub.org/public/SEGA/SEGA101_seq/1

rrnC
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
label: rrnC
note: rrnC
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA101/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA101/annotation8/range/1

https://synbiohub.org/public/SEGA/SEGA101/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA101/1
start: 3835
end: 3875
orientation: inline

L3S2P11 Terminator
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
label: L3S2P11 Terminator
note: L3S2P11 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA101/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA101/annotation1/range/1

https://synbiohub.org/public/SEGA/SEGA101/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA101/1
start: 538
end: 594
orientation: inline

glmS
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
codon start: 1
label: glmS
note: glmS
translation: VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA101/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA101/annotation0/range/1

https://synbiohub.org/public/SEGA/SEGA101/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA101/1
start: 1
end: 528
orientation: inline

TcR
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
codon start: 1
gene: tet
label: TcR
note: TcR
note: confers resistance to tetracycline
product: tetracycline efflux protein
translation: VLPGLLRDIVHSDSIASHYGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVIFGEDRFRWSATMSGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA101/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA101/annotation7/range/1

https://synbiohub.org/public/SEGA/SEGA101/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA101/1
start: 2712
end: 3824
orientation: inline

rhaS
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
codon start: 1
gene: E. coli rhaS
label: rhaS
note: activated by L-rhamnose
note: rhaS
product: positive regulator of the rhaB promoter
translation: MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHGTGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLLPQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKSSLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQRYLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGFLQ
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA101/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA101/annotation3/range/1

https://synbiohub.org/public/SEGA/SEGA101/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA101/1
start: 1526
end: 2362
orientation: reverseComplement

rhaR
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
codon start: 1
gene: E. coli rhaR
label: rhaR
note: needed for rhamnose-dependent activation of the rhaB promoter
note: rhaR
product: transcriptional activator of rhaR and rhaS
translation: MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQGAIPGFNASAGQPHWRLGSMGMAQARQVIGQLEHESSQHVPFANEMAELLFGQLVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRHLNSQKD
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA101/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA101/annotation2/range/1

https://synbiohub.org/public/SEGA/SEGA101/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA101/1
start: 607
end: 1452
orientation: reverseComplement

pstS
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
codon start: 1
label: pstS
note: pstS
translation: MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKWADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA101/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA101/annotation9/range/1

https://synbiohub.org/public/SEGA/SEGA101/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA101/1
start: 4070
end: 4317
orientation: inline

BCD
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
codon start: 1
label: BCD
note: BCD
translation: MKAIFVLKHLNHAKEVF
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA101/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA101/annotation6/range/1

https://synbiohub.org/public/SEGA/SEGA101/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA101/1
start: 2656
end: 2711
orientation: inline

rhaB promoter
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
label: rhaB promoter
note: promoter of the E. coli rhaBAD operon, conferring tight induction with L-rhamnose and repression with D-glucose in the presence of RhaR and RhaS (Giacalone et al., 2006)
note: rhaB promoter
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA101/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA101/annotation4/range/1

https://synbiohub.org/public/SEGA/SEGA101/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA101/1
start: 2508
end: 2626
orientation: inline

low TIR
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
note: TIR
note: low TIR
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA101/1
role: SO:0000139
location: https://synbiohub.org/public/SEGA/SEGA101/annotation5/range/1

https://synbiohub.org/public/SEGA/SEGA101/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA101/1
start: 2638
end: 2664
orientation: inline

https://synbiohub.org/public/SEGA/SEGA010/1
type: ComponentDefinition
persistent identity: SEGA010
display id: SEGA010
version: 1
description: natural linear DNA.
owned by: carbay
owned by: morno
date: 09-SEP-2021
division: UNA
keywords: .
locus: SEGA010
molecule: ds-DNA
organism: unspecified
reference: https://synbiohub.org/user/carbay/SEGA/SEGA010_reference0/1
source: natural DNA sequence
type: DnaRegion
type: SO:0000987
role: SO:0000804
sequence annotation: GFP
sequence annotation: rrnC
sequence annotation: TcR
sequence annotation: pstS
sequence annotation: glmS
sequence annotation: P-J23100
sequence annotation: high TIR
sequence annotation: Ubiquitin
sequence annotation: L3S3P22 Terminator (synthetic)
sequence annotation: L3S2P11 Terminator
sequence annotation: Promoter 3BCD2
sequence: https://synbiohub.org/public/SEGA/SEGA010_seq/1

GFP
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
codon start: 1
label: GFP
note: GFP
product: green fluorescent protein
translation: SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYK
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA010/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA010/annotation5/range/1

https://synbiohub.org/public/SEGA/SEGA010/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA010/1
start: 977
end: 1687
orientation: inline

rrnC
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
label: rrnC
note: rrnC
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA010/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA010/annotation9/range/1

https://synbiohub.org/public/SEGA/SEGA010/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA010/1
start: 3019
end: 3059
orientation: inline

TcR
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
codon start: 1
gene: tet
label: TcR
note: TcR
note: confers resistance to tetracycline
product: tetracycline efflux protein
translation: MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHYGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVIFGEDRFRWSATMSGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA010/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA010/annotation8/range/1

https://synbiohub.org/public/SEGA/SEGA010/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA010/1
start: 1818
end: 3008
orientation: inline

pstS
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
codon start: 1
label: pstS
note: pstS
translation: MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKWADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA010/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA010/annotation10/range/1

https://synbiohub.org/public/SEGA/SEGA010/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA010/1
start: 3254
end: 3501
orientation: inline

glmS
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
codon start: 1
label: glmS
note: glmS
translation: VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA010/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA010/annotation0/range/1

https://synbiohub.org/public/SEGA/SEGA010/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA010/1
start: 1
end: 528
orientation: inline

P-J23100
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
label: P-J23100
note: P-J23100
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA010/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA010/annotation2/range/1

https://synbiohub.org/public/SEGA/SEGA010/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA010/1
start: 604
end: 638
orientation: inline

high TIR
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
note: TIR
note: high TIR
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA010/1
role: SO:0000139
location: https://synbiohub.org/public/SEGA/SEGA010/annotation3/range/1

https://synbiohub.org/public/SEGA/SEGA010/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA010/1
start: 650
end: 676
orientation: inline

Ubiquitin
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
codon start: 1
label: Ubiquitin
note: Ubiquitin
translation: MQIFVKTLTGKTITLEVEPGSAHHHHHHHHHHAGSSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQEKSTLELVLELQNETQSATSDA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA010/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA010/annotation4/range/1

https://synbiohub.org/public/SEGA/SEGA010/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA010/1
start: 668
end: 967
orientation: inline

L3S3P22 Terminator (synthetic)
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
label: L3S3P22 Terminator (synthetic)
note: L3S3P22 Terminator (synthetic)
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA010/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA010/annotation6/range/1

https://synbiohub.org/public/SEGA/SEGA010/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA010/1
start: 1691
end: 1739
orientation: inline

L3S2P11 Terminator
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
label: L3S2P11 Terminator
note: L3S2P11 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA010/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA010/annotation1/range/1

https://synbiohub.org/public/SEGA/SEGA010/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA010/1
start: 538
end: 594
orientation: inline

Promoter 3BCD2
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
label: Promoter 3BCD2
note: Promoter 3BCD2
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA010/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA010/annotation7/range/1

https://synbiohub.org/public/SEGA/SEGA010/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA010/1
start: 1750
end: 1778
orientation: inline

https://synbiohub.org/public/SEGA/SEGA086/1
type: ComponentDefinition
persistent identity: SEGA086
display id: SEGA086
version: 1
description: natural linear DNA.
owned by: carbay
owned by: morno
date: 09-SEP-2021
division: UNA
keywords: .
locus: SEGA086
molecule: ds-DNA
organism: unspecified
reference: https://synbiohub.org/public/SEGA/reference0_1/1
source: natural DNA sequence
type: DnaRegion
type: SO:0000987
role: SO:0000804
sequence annotation: rhaB promoter
sequence annotation: Promoter 3BCD2
sequence annotation: rhaS
sequence annotation: galK
sequence annotation: GFP
sequence annotation: rhaR
sequence annotation: Terminator rrnB T1
sequence annotation: rrnC
sequence annotation: L3S3P22 Terminator (synthetic)
sequence annotation: glmS
sequence annotation: pstS
sequence annotation: L3S2P11 Terminator
sequence annotation: TEV site
sequence annotation: TcR
sequence annotation: EM7 promoter
sequence annotation: high TIR
sequence: https://synbiohub.org/public/SEGA/SEGA086_seq/1

rhaB promoter
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
label: rhaB promoter
note: promoter of the E. coli rhaBAD operon, conferring tight induction with L-rhamnose and repression with D-glucose in the presence of RhaR and RhaS (Giacalone et al., 2006)
note: rhaB promoter
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA086/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA086/annotation7/range/1

https://synbiohub.org/public/SEGA/SEGA086/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA086/1
start: 3800
end: 3918
orientation: inline

Promoter 3BCD2
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
label: Promoter 3BCD2
note: Promoter 3BCD2
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA086/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA086/annotation12/range/1

https://synbiohub.org/public/SEGA/SEGA086/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA086/1
start: 4742
end: 4770
orientation: inline

rhaS
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
codon start: 1
gene: E. coli rhaS
label: rhaS
note: activated by L-rhamnose
note: rhaS
product: positive regulator of the rhaB promoter
translation: MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHGTGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLLPQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKSSLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQRYLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGFLQ
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA086/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA086/annotation6/range/1

https://synbiohub.org/public/SEGA/SEGA086/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA086/1
start: 2818
end: 3654
orientation: reverseComplement

galK
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
EC number: 2.7.1.6
codon start: 1
db xref: ASAP:ABE-0002568
db xref: EcoGene:EG10363
db xref: GI:16128725
db xref: GeneID:945358
db xref: UniProtKB/Swiss-Prot:P0A6T3
function: enzyme; Degradation of small molecules: Carbon compounds
gene: galK
gene synonym: ECK0746; galA; JW0740
label: galK
locus tag: b0757
note: galK
product: galactokinase
protein id: NP_415278.1
translation: MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVLPCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLDAPIVAHENYQWANYVRGVVKHLQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQEAENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTLVGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTVEAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGGGFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA086/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA086/annotation2/range/1

https://synbiohub.org/public/SEGA/SEGA086/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA086/1
start: 605
end: 1753
orientation: reverseComplement

GFP
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
codon start: 1
label: GFP
note: GFP
product: green fluorescent protein
translation: SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSTLSKDPNEKRDHMVLLEFVTAAGITHGMDELYK
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA086/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA086/annotation10/range/1

https://synbiohub.org/public/SEGA/SEGA086/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA086/1
start: 3969
end: 4679
orientation: inline

rhaR
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
codon start: 1
gene: E. coli rhaR
label: rhaR
note: needed for rhamnose-dependent activation of the rhaB promoter
note: rhaR
product: transcriptional activator of rhaR and rhaS
translation: MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQGAIPGFNASAGQPHWRLGSMGMAQARQVIGQLEHESSQHVPFANEMAELLFGQLVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRHLNSQKD
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA086/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA086/annotation5/range/1

https://synbiohub.org/public/SEGA/SEGA086/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA086/1
start: 1899
end: 2744
orientation: reverseComplement

Terminator rrnB T1
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
label: Terminator rrnB T1
note: Terminator rrnB T1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA086/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA086/annotation4/range/1

https://synbiohub.org/public/SEGA/SEGA086/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA086/1
start: 1843
end: 1886
orientation: inline

rrnC
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
label: rrnC
note: rrnC
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA086/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA086/annotation14/range/1

https://synbiohub.org/public/SEGA/SEGA086/annotation14/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA086/1
start: 6011
end: 6051
orientation: inline

L3S3P22 Terminator (synthetic)
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
label: L3S3P22 Terminator (synthetic)
note: L3S3P22 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA086/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA086/annotation11/range/1

https://synbiohub.org/public/SEGA/SEGA086/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA086/1
start: 4683
end: 4731
orientation: inline

glmS
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
codon start: 1
label: glmS
note: glmS
translation: VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA086/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA086/annotation0/range/1

https://synbiohub.org/public/SEGA/SEGA086/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA086/1
start: 1
end: 528
orientation: inline

pstS
type: SequenceAnnotation
persistent identity: annotation15
display id: annotation15
version: 1
codon start: 1
label: pstS
note: pstS
translation: MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKWADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA086/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA086/annotation15/range/1

https://synbiohub.org/public/SEGA/SEGA086/annotation15/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA086/1
start: 6246
end: 6493
orientation: inline

L3S2P11 Terminator
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
label: L3S2P11 Terminator
note: L3S2P11 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA086/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA086/annotation1/range/1

https://synbiohub.org/public/SEGA/SEGA086/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA086/1
start: 538
end: 594
orientation: inline

TEV site
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
codon start: 1
label: TEV site
note: TEV site
product: tobacco etch virus (TEV) protease recognition and cleavage site
translation: MENLYFQ
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA086/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA086/annotation9/range/1

https://synbiohub.org/public/SEGA/SEGA086/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA086/1
start: 3948
end: 3968
orientation: inline

TcR
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
codon start: 1
gene: tet
label: TcR
note: TcR
note: confers resistance to tetracycline
product: tetracycline efflux protein
translation: MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHYGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA086/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA086/annotation13/range/1

https://synbiohub.org/public/SEGA/SEGA086/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA086/1
start: 4810
end: 6000
orientation: inline

EM7 promoter
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
label: EM7 promoter
note: EM7 promoter
note: synthetic bacterial promoter
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA086/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA086/annotation3/range/1

https://synbiohub.org/public/SEGA/SEGA086/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA086/1
start: 1783
end: 1830
orientation: reverseComplement

high TIR
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
note: TIR
note: high TIR
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA086/1
role: SO:0000139
location: https://synbiohub.org/public/SEGA/SEGA086/annotation8/range/1

https://synbiohub.org/public/SEGA/SEGA086/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA086/1
start: 3930
end: 3956
orientation: inline

https://synbiohub.org/public/SEGA/SEGA002/1
type: ComponentDefinition
persistent identity: SEGA002
display id: SEGA002
version: 1
description: natural linear DNA.
owned by: carbay
owned by: morno
date: 08-SEP-2021
division: UNA
keywords: .
locus: SEGA002
molecule: ds-DNA
organism: unspecified
reference: https://synbiohub.org/user/carbay/SEGA/SEGA002_reference0/1
source: natural DNA sequence
type: DnaRegion
type: SO:0000987
role: SO:0000804
sequence annotation: TcR
sequence annotation: pstS
sequence annotation: rrnC
sequence annotation: L3S2P11 Terminator
sequence annotation: lacI
sequence annotation: RBS
sequence annotation: glmS
sequence annotation: lacI promoter
sequence annotation: trc promoter
sequence: https://synbiohub.org/public/SEGA/SEGA002_seq/1

TcR
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
codon start: 1
gene: tet
label: TcR
note: TcR
note: confers resistance to tetracycline
product: tetracycline efflux protein
translation: VLPGLLRDIVHSDSIASHYGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVIFGEDRFRWSATMSGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA002/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA002/annotation6/range/1

https://synbiohub.org/public/SEGA/SEGA002/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA002/1
start: 1872
end: 2984
orientation: inline

pstS
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
codon start: 1
label: pstS
note: pstS
translation: MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKWADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA002/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA002/annotation8/range/1

https://synbiohub.org/public/SEGA/SEGA002/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA002/1
start: 3230
end: 3477
orientation: inline

rrnC
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
label: rrnC
note: rrnC
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA002/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA002/annotation7/range/1

https://synbiohub.org/public/SEGA/SEGA002/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA002/1
start: 2995
end: 3035
orientation: inline

L3S2P11 Terminator
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
label: L3S2P11 Terminator
note: L3S2P11 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA002/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA002/annotation1/range/1

https://synbiohub.org/public/SEGA/SEGA002/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA002/1
start: 538
end: 594
orientation: inline

lacI
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
codon start: 1
gene: lacI
label: lacI
note: The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG).
note: lacI
product: lac repressor
translation: MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA002/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA002/annotation2/range/1

https://synbiohub.org/public/SEGA/SEGA002/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA002/1
start: 604
end: 1686
orientation: reverseComplement

RBS
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
note: RBS
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA002/1
role: SO:0000139
location: https://synbiohub.org/public/SEGA/SEGA002/annotation5/range/1

https://synbiohub.org/public/SEGA/SEGA002/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA002/1
start: 1860
end: 1871
orientation: inline

glmS
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
codon start: 1
label: glmS
note: glmS
translation: VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA002/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA002/annotation0/range/1

https://synbiohub.org/public/SEGA/SEGA002/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA002/1
start: 1
end: 528
orientation: inline

lacI promoter
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
gene: lacI
label: lacI promoter
note: lacI promoter
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA002/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA002/annotation3/range/1

https://synbiohub.org/public/SEGA/SEGA002/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA002/1
start: 1687
end: 1764
orientation: reverseComplement

trc promoter
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
label: trc promoter
note: strong E. coli promoter; hybrid between the trp and lac UV5 promoters
note: trc promoter
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA002/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA002/annotation4/range/1

https://synbiohub.org/public/SEGA/SEGA002/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA002/1
start: 1795
end: 1824
orientation: inline

https://synbiohub.org/public/SEGA/SEGA082/1
type: ComponentDefinition
persistent identity: SEGA082
display id: SEGA082
version: 1
description: natural linear DNA.
owned by: carbay
owned by: morno
date: 09-SEP-2021
division: UNA
keywords: .
locus: SEGA082
molecule: ds-DNA
organism: unspecified
reference: https://synbiohub.org/public/SEGA/reference0_1/1
source: natural DNA sequence
type: DnaRegion
type: SO:0000987
role: SO:0000804
sequence annotation: rhaS
sequence annotation: EM7 promoter
sequence annotation: rhaB promoter
sequence annotation: pstS
sequence annotation: Terminator rrnB T1
sequence annotation: rrnC
sequence annotation: L3S3P22 Terminator (synthetic)
sequence annotation: TcR
sequence annotation: L3S2P11 Terminator
sequence annotation: glmS
sequence annotation: Ubiquitin
sequence annotation: GFP
sequence annotation: medium TIR
sequence annotation: rhaR
sequence annotation: Promoter 3BCD2
sequence annotation: galK
sequence: https://synbiohub.org/public/SEGA/SEGA082_seq/1

rhaS
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
codon start: 1
gene: E. coli rhaS
label: rhaS
note: activated by L-rhamnose
note: rhaS
product: positive regulator of the rhaB promoter
translation: MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHGTGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLLPQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKSSLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQRYLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGFLQ
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA082/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA082/annotation6/range/1

https://synbiohub.org/public/SEGA/SEGA082/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA082/1
start: 2818
end: 3654
orientation: reverseComplement

EM7 promoter
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
label: EM7 promoter
note: EM7 promoter
note: synthetic bacterial promoter
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA082/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA082/annotation3/range/1

https://synbiohub.org/public/SEGA/SEGA082/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA082/1
start: 1783
end: 1830
orientation: reverseComplement

rhaB promoter
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
label: rhaB promoter
note: promoter of the E. coli rhaBAD operon, conferring tight induction with L-rhamnose and repression with D-glucose in the presence of RhaR and RhaS (Giacalone et al., 2006)
note: rhaB promoter
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA082/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA082/annotation7/range/1

https://synbiohub.org/public/SEGA/SEGA082/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA082/1
start: 3800
end: 3918
orientation: inline

pstS
type: SequenceAnnotation
persistent identity: annotation15
display id: annotation15
version: 1
codon start: 1
label: pstS
note: pstS
translation: MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKWADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA082/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA082/annotation15/range/1

https://synbiohub.org/public/SEGA/SEGA082/annotation15/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA082/1
start: 6534
end: 6781
orientation: inline

Terminator rrnB T1
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
label: Terminator rrnB T1
note: Terminator rrnB T1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA082/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA082/annotation4/range/1

https://synbiohub.org/public/SEGA/SEGA082/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA082/1
start: 1843
end: 1886
orientation: inline

rrnC
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
label: rrnC
note: rrnC
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA082/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA082/annotation14/range/1

https://synbiohub.org/public/SEGA/SEGA082/annotation14/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA082/1
start: 6299
end: 6339
orientation: inline

L3S3P22 Terminator (synthetic)
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
label: L3S3P22 Terminator (synthetic)
note: L3S3P22 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA082/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA082/annotation11/range/1

https://synbiohub.org/public/SEGA/SEGA082/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA082/1
start: 4971
end: 5019
orientation: inline

TcR
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
codon start: 1
gene: tet
label: TcR
note: TcR
note: confers resistance to tetracycline
product: tetracycline efflux protein
translation: MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHYGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA082/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA082/annotation13/range/1

https://synbiohub.org/public/SEGA/SEGA082/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA082/1
start: 5098
end: 6288
orientation: inline

L3S2P11 Terminator
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
label: L3S2P11 Terminator
note: L3S2P11 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA082/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA082/annotation1/range/1

https://synbiohub.org/public/SEGA/SEGA082/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA082/1
start: 538
end: 594
orientation: inline

glmS
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
codon start: 1
label: glmS
note: glmS
translation: VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA082/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA082/annotation0/range/1

https://synbiohub.org/public/SEGA/SEGA082/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA082/1
start: 1
end: 528
orientation: inline

Ubiquitin
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
codon start: 1
label: Ubiquitin
note: Ubiquitin
translation: MQIFVKTLTGKTITLEVEPGSAHHHHHHHHHHAGSSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQEKSTLELVLELQNETQSATSDA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA082/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA082/annotation9/range/1

https://synbiohub.org/public/SEGA/SEGA082/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA082/1
start: 3948
end: 4247
orientation: inline

GFP
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
codon start: 1
label: GFP
note: GFP
product: green fluorescent protein
translation: SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYK
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA082/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA082/annotation10/range/1

https://synbiohub.org/public/SEGA/SEGA082/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA082/1
start: 4257
end: 4967
orientation: inline

medium TIR
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
note: TIR
note: medium TIR
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA082/1
role: SO:0000139
location: https://synbiohub.org/public/SEGA/SEGA082/annotation8/range/1

https://synbiohub.org/public/SEGA/SEGA082/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA082/1
start: 3930
end: 3956
orientation: inline

rhaR
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
codon start: 1
gene: E. coli rhaR
label: rhaR
note: needed for rhamnose-dependent activation of the rhaB promoter
note: rhaR
product: transcriptional activator of rhaR and rhaS
translation: MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQGAIPGFNASAGQPHWRLGSMGMAQARQVIGQLEHESSQHVPFANEMAELLFGQLVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRHLNSQKD
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA082/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA082/annotation5/range/1

https://synbiohub.org/public/SEGA/SEGA082/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA082/1
start: 1899
end: 2744
orientation: reverseComplement

Promoter 3BCD2
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
label: Promoter 3BCD2
note: Promoter 3BCD2
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA082/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA082/annotation12/range/1

https://synbiohub.org/public/SEGA/SEGA082/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA082/1
start: 5030
end: 5058
orientation: inline

galK
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
EC number: 2.7.1.6
codon start: 1
db xref: ASAP:ABE-0002568
db xref: EcoGene:EG10363
db xref: GI:16128725
db xref: GeneID:945358
db xref: UniProtKB/Swiss-Prot:P0A6T3
function: enzyme; Degradation of small molecules: Carbon compounds
gene: galK
gene synonym: ECK0746; galA; JW0740
label: galK
locus tag: b0757
note: galK
product: galactokinase
protein id: NP_415278.1
translation: MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVLPCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLDAPIVAHENYQWANYVRGVVKHLQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQEAENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTLVGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTVEAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGGGFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA082/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA082/annotation2/range/1

https://synbiohub.org/public/SEGA/SEGA082/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA082/1
start: 605
end: 1753
orientation: reverseComplement

https://synbiohub.org/public/SEGA/SEGA055/1
type: ComponentDefinition
persistent identity: SEGA055
display id: SEGA055
version: 1
description: natural linear DNA.
owned by: carbay
owned by: morno
date: 08-SEP-2021
division: UNA
keywords: .
locus: SEGA055
molecule: ds-DNA
organism: unspecified
reference: https://synbiohub.org/user/carbay/SEGA/SEGA055_reference0/1
source: natural DNA sequence
type: DnaRegion
type: SO:0000987
role: SO:0000804
sequence annotation: yebF
sequence annotation: pstS
sequence annotation: Promoter 3BCD2
sequence annotation: glmS
sequence annotation: rhaR
sequence annotation: YebF
sequence annotation: L3S3P22 Terminator (synthetic)
sequence annotation: rhaS
sequence annotation: rhaB promoter
sequence annotation: rrnC
sequence annotation: high TIR
sequence annotation: sfGFP
sequence annotation: L3S2P11 Terminator
sequence annotation: TcR
sequence: https://synbiohub.org/public/SEGA/SEGA055_seq/1

yebF
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
codon start: 1
db xref: ASAP:ABE-0006141
db xref: EcoGene:EG11807
db xref: GI:145698278
db xref: GeneID:946363
db xref: UniProtKB/Swiss-Prot:P33219
gene: yebF
gene synonym: ECK1848; JW1836
label: yebF
locus tag: b1847
note: yebFss
product: yebF signal sequence
protein id: NP_416361.2
translation: MKKRGAFLGLLLVSACASVFA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA055/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA055/annotation6/range/1

https://synbiohub.org/public/SEGA/SEGA055/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA055/1
start: 2656
end: 2718
orientation: inline

pstS
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
codon start: 1
label: pstS
note: pstS
translation: MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKWADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA055/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA055/annotation13/range/1

https://synbiohub.org/public/SEGA/SEGA055/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA055/1
start: 5287
end: 5534
orientation: inline

Promoter 3BCD2
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
label: Promoter 3BCD2
note: Promoter 3BCD2
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA055/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA055/annotation10/range/1

https://synbiohub.org/public/SEGA/SEGA055/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA055/1
start: 3783
end: 3811
orientation: inline

glmS
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
codon start: 1
label: glmS
note: glmS
translation: VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA055/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA055/annotation0/range/1

https://synbiohub.org/public/SEGA/SEGA055/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA055/1
start: 1
end: 528
orientation: inline

rhaR
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
codon start: 1
gene: E. coli rhaR
label: rhaR
note: needed for rhamnose-dependent activation of the rhaB promoter
note: rhaR
product: transcriptional activator of rhaR and rhaS
translation: MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQGAIPGFNASAGQPHWRLGSMGMAQARQVIGQLEHESSQHVPFANEMAELLFGQLVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRHLNSQKD
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA055/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA055/annotation2/range/1

https://synbiohub.org/public/SEGA/SEGA055/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA055/1
start: 607
end: 1452
orientation: reverseComplement

YebF
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
codon start: 1
label: YebF
note: YebF
product: full size yebF protein
translation: ANNETSKSVTFPKCEDLDAAGIAASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHYQVSVDCKAGMAEYQRR
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA055/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA055/annotation7/range/1

https://synbiohub.org/public/SEGA/SEGA055/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA055/1
start: 2719
end: 3009
orientation: inline

L3S3P22 Terminator (synthetic)
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
label: L3S3P22 Terminator (synthetic)
note: L3S3P22 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA055/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA055/annotation9/range/1

https://synbiohub.org/public/SEGA/SEGA055/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA055/1
start: 3724
end: 3772
orientation: inline

rhaS
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
codon start: 1
gene: E. coli rhaS
label: rhaS
note: activated by L-rhamnose
note: rhaS
product: positive regulator of the rhaB promoter
translation: MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHGTGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLLPQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKSSLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQRYLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGFLQ
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA055/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA055/annotation3/range/1

https://synbiohub.org/public/SEGA/SEGA055/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA055/1
start: 1526
end: 2362
orientation: reverseComplement

rhaB promoter
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
label: rhaB promoter
note: promoter of the E. coli rhaBAD operon, conferring tight induction with L-rhamnose and repression with D-glucose in the presence of RhaR and RhaS (Giacalone et al., 2006)
note: rhaB promoter
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA055/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA055/annotation4/range/1

https://synbiohub.org/public/SEGA/SEGA055/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA055/1
start: 2508
end: 2626
orientation: inline

rrnC
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
label: rrnC
note: rrnC
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA055/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA055/annotation12/range/1

https://synbiohub.org/public/SEGA/SEGA055/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA055/1
start: 5052
end: 5092
orientation: inline

high TIR
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
note: TIR
note: high TIR
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA055/1
role: SO:0000139
location: https://synbiohub.org/public/SEGA/SEGA055/annotation5/range/1

https://synbiohub.org/public/SEGA/SEGA055/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA055/1
start: 2638
end: 2664
orientation: inline

sfGFP
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
codon start: 1
label: sfGFP
note: sfGFP
product: superfolder GFP
translation: RKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDGTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEFVTAAGITHGMDELYK
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA055/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA055/annotation8/range/1

https://synbiohub.org/public/SEGA/SEGA055/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA055/1
start: 3010
end: 3720
orientation: inline

L3S2P11 Terminator
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
label: L3S2P11 Terminator
note: L3S2P11 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA055/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA055/annotation1/range/1

https://synbiohub.org/public/SEGA/SEGA055/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA055/1
start: 538
end: 594
orientation: inline

TcR
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
codon start: 1
gene: tet
label: TcR
note: TcR
note: confers resistance to tetracycline
product: tetracycline efflux protein
translation: MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHYGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVIFGEDRFRWSATMSGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA055/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA055/annotation11/range/1

https://synbiohub.org/public/SEGA/SEGA055/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA055/1
start: 3851
end: 5041
orientation: inline

https://synbiohub.org/public/SEGA/SEGA063/1
type: ComponentDefinition
persistent identity: SEGA063
display id: SEGA063
version: 1
description: natural linear DNA.
owned by: carbay
owned by: morno
date: 08-SEP-2021
division: UNA
keywords: .
locus: SEGA063
molecule: ds-DNA
organism: unspecified
reference: https://synbiohub.org/user/carbay/SEGA/SEGA063_reference0/1
source: natural DNA sequence
type: DnaRegion
type: SO:0000987
role: SO:0000804
sequence annotation: T7 promoter
sequence annotation: TEV site
sequence annotation: TcR
sequence annotation: Promoter 3BCD2
sequence annotation: rrnC
sequence annotation: glmS
sequence annotation: GFP
sequence annotation: L3S2P11 Terminator
sequence annotation: pstS
sequence annotation: lacI
sequence annotation: medium TIR
sequence annotation: lac operator
sequence annotation: L3S3P22 Terminator (synthetic)
sequence annotation: lacI promoter
sequence: https://synbiohub.org/public/SEGA/SEGA063_seq/1

T7 promoter
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
label: T7 promoter
note: T7 promoter
note: promoter for bacteriophage T7 RNA polymerase
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA063/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA063/annotation4/range/1

https://synbiohub.org/public/SEGA/SEGA063/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA063/1
start: 1795
end: 1813
orientation: inline

TEV site
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
codon start: 1
label: TEV site
note: TEV site
product: tobacco etch virus (TEV) protease recognition and cleavage site
translation: MENLYFQ
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA063/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA063/annotation7/range/1

https://synbiohub.org/public/SEGA/SEGA063/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA063/1
start: 1868
end: 1888
orientation: inline

TcR
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
codon start: 1
gene: tet
label: TcR
note: TcR
note: confers resistance to tetracycline
product: tetracycline efflux protein
translation: MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHYGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVIFGEDRFRWSATMSGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA063/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA063/annotation11/range/1

https://synbiohub.org/public/SEGA/SEGA063/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA063/1
start: 2730
end: 3920
orientation: inline

Promoter 3BCD2
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
label: Promoter 3BCD2
note: Promoter 3BCD2
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA063/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA063/annotation10/range/1

https://synbiohub.org/public/SEGA/SEGA063/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA063/1
start: 2662
end: 2690
orientation: inline

rrnC
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
label: rrnC
note: rrnC
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA063/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA063/annotation12/range/1

https://synbiohub.org/public/SEGA/SEGA063/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA063/1
start: 3931
end: 3971
orientation: inline

glmS
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
codon start: 1
label: glmS
note: glmS
translation: VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA063/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA063/annotation0/range/1

https://synbiohub.org/public/SEGA/SEGA063/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA063/1
start: 1
end: 528
orientation: inline

GFP
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
codon start: 1
label: GFP
note: GFP
product: green fluorescent protein
translation: SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYK
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA063/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA063/annotation8/range/1

https://synbiohub.org/public/SEGA/SEGA063/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA063/1
start: 1889
end: 2599
orientation: inline

L3S2P11 Terminator
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
label: L3S2P11 Terminator
note: L3S2P11 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA063/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA063/annotation1/range/1

https://synbiohub.org/public/SEGA/SEGA063/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA063/1
start: 538
end: 594
orientation: inline

pstS
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
codon start: 1
label: pstS
note: pstS
translation: MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKWADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA063/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA063/annotation13/range/1

https://synbiohub.org/public/SEGA/SEGA063/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA063/1
start: 4166
end: 4413
orientation: inline

lacI
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
codon start: 1
gene: lacI
label: lacI
note: The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG).
note: lacI
product: lac repressor
translation: MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA063/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA063/annotation2/range/1

https://synbiohub.org/public/SEGA/SEGA063/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA063/1
start: 604
end: 1686
orientation: reverseComplement

medium TIR
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
note: TIR
note: medium TIR
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA063/1
role: SO:0000139
location: https://synbiohub.org/public/SEGA/SEGA063/annotation6/range/1

https://synbiohub.org/public/SEGA/SEGA063/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA063/1
start: 1850
end: 1876
orientation: inline

lac operator
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
bound moiety: lac repressor encoded by lacI
feature type: protein_bind
label: lac operator
note: The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG).
note: lac operator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA063/1
role: SO:0000110
location: https://synbiohub.org/public/SEGA/SEGA063/annotation5/range/1

https://synbiohub.org/public/SEGA/SEGA063/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA063/1
start: 1814
end: 1838
orientation: inline

L3S3P22 Terminator (synthetic)
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
label: L3S3P22 Terminator (synthetic)
note: L3S3P22 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA063/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA063/annotation9/range/1

https://synbiohub.org/public/SEGA/SEGA063/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA063/1
start: 2603
end: 2651
orientation: inline

lacI promoter
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
gene: lacI
label: lacI promoter
note: lacI promoter
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA063/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA063/annotation3/range/1

https://synbiohub.org/public/SEGA/SEGA063/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA063/1
start: 1687
end: 1764
orientation: reverseComplement

https://synbiohub.org/public/SEGA/SEGA099/1
type: ComponentDefinition
persistent identity: SEGA099
display id: SEGA099
version: 1
description: natural linear DNA.
owned by: carbay
owned by: morno
date: 09-SEP-2021
division: UNA
keywords: .
locus: SEGA099
molecule: ds-DNA
organism: unspecified
reference: https://synbiohub.org/user/carbay/SEGA/SEGA099_reference0/1
source: natural DNA sequence
type: DnaRegion
type: SO:0000987
role: SO:0000804
sequence annotation: trc promoter
sequence annotation: BCD
sequence annotation: lacI promoter
sequence annotation: L3S2P11 Terminator
sequence annotation: lacO
sequence annotation: lacI
sequence annotation: TcR
sequence annotation: low TIR
sequence annotation: glmS
sequence annotation: rrnC
sequence annotation: pstS
sequence: https://synbiohub.org/public/SEGA/SEGA099_seq/1

trc promoter
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
label: trc promoter
note: strong E. coli promoter; hybrid between the trp and lac UV5 promoters
note: trc promoter
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA099/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA099/annotation4/range/1

https://synbiohub.org/public/SEGA/SEGA099/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA099/1
start: 1795
end: 1824
orientation: inline

BCD
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
codon start: 1
label: BCD
note: BCD
translation: MKAIFVLKHLNHAKEVF
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA099/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA099/annotation7/range/1

https://synbiohub.org/public/SEGA/SEGA099/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA099/1
start: 1878
end: 1933
orientation: inline

lacI promoter
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
gene: lacI
label: lacI promoter
note: lacI promoter
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA099/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA099/annotation3/range/1

https://synbiohub.org/public/SEGA/SEGA099/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA099/1
start: 1687
end: 1764
orientation: reverseComplement

L3S2P11 Terminator
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
label: L3S2P11 Terminator
note: L3S2P11 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA099/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA099/annotation1/range/1

https://synbiohub.org/public/SEGA/SEGA099/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA099/1
start: 538
end: 594
orientation: inline

lacO
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
bound moiety: lac repressor
feature type: protein_bind
label: lacO
note: lac operator
note: lacO
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA099/1
role: SO:0000110
location: https://synbiohub.org/public/SEGA/SEGA099/annotation5/range/1

https://synbiohub.org/public/SEGA/SEGA099/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA099/1
start: 1825
end: 1848
orientation: inline

lacI
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
codon start: 1
gene: lacI
label: lacI
note: The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG).
note: lacI
product: lac repressor
translation: MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA099/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA099/annotation2/range/1

https://synbiohub.org/public/SEGA/SEGA099/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA099/1
start: 604
end: 1686
orientation: reverseComplement

TcR
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
codon start: 1
gene: tet
label: TcR
note: TcR
note: confers resistance to tetracycline
product: tetracycline efflux protein
translation: VLPGLLRDIVHSDSIASHYGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVIFGEDRFRWSATMSGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA099/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA099/annotation8/range/1

https://synbiohub.org/public/SEGA/SEGA099/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA099/1
start: 1934
end: 3046
orientation: inline

low TIR
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
note: TIR
note: low TIR
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA099/1
role: SO:0000139
location: https://synbiohub.org/public/SEGA/SEGA099/annotation6/range/1

https://synbiohub.org/public/SEGA/SEGA099/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA099/1
start: 1860
end: 1886
orientation: inline

glmS
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
codon start: 1
label: glmS
note: glmS
translation: VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA099/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA099/annotation0/range/1

https://synbiohub.org/public/SEGA/SEGA099/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA099/1
start: 1
end: 528
orientation: inline

rrnC
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
label: rrnC
note: rrnC
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA099/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA099/annotation9/range/1

https://synbiohub.org/public/SEGA/SEGA099/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA099/1
start: 3057
end: 3097
orientation: inline

pstS
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
codon start: 1
label: pstS
note: pstS
translation: MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKWADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA099/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA099/annotation10/range/1

https://synbiohub.org/public/SEGA/SEGA099/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA099/1
start: 3292
end: 3539
orientation: inline

https://synbiohub.org/public/SEGA/SEGA089/1
type: ComponentDefinition
persistent identity: SEGA089
display id: SEGA089
version: 1
description: natural linear DNA.
owned by: carbay
owned by: morno
date: 09-SEP-2021
division: UNA
keywords: .
locus: SEGA089
molecule: ds-DNA
organism: unspecified
reference: https://synbiohub.org/user/carbay/SEGA/SEGA089_reference0/1
source: natural DNA sequence
type: DnaRegion
type: SO:0000987
role: SO:0000804
sequence annotation: rhaS
sequence annotation: GFP
sequence annotation: Terminator rrnB T1
sequence annotation: L3S2P11 Terminator
sequence annotation: high TIR
sequence annotation: TcR
sequence annotation: EM7 promoter
sequence annotation: pstS
sequence annotation: L3S3P22 Terminator (synthetic)
sequence annotation: PROTi-Linker
sequence annotation: Promoter 3BCD2
sequence annotation: TEV site
sequence annotation: rhaR
sequence annotation: glmS
sequence annotation: rhaB promoter
sequence annotation: galK
sequence annotation: rrnC
sequence: https://synbiohub.org/public/SEGA/SEGA089_seq/1

rhaS
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
codon start: 1
gene: E. coli rhaS
label: rhaS
note: activated by L-rhamnose
note: rhaS
product: positive regulator of the rhaB promoter
translation: MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHGTGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLLPQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKSSLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQRYLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGFLQ
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA089/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA089/annotation6/range/1

https://synbiohub.org/public/SEGA/SEGA089/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA089/1
start: 2818
end: 3654
orientation: reverseComplement

GFP
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
codon start: 1
label: GFP
note: GFP
product: green fluorescent protein
translation: SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYK
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA089/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA089/annotation11/range/1

https://synbiohub.org/public/SEGA/SEGA089/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA089/1
start: 4005
end: 4715
orientation: inline

Terminator rrnB T1
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
label: Terminator rrnB T1
note: Terminator rrnB T1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA089/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA089/annotation4/range/1

https://synbiohub.org/public/SEGA/SEGA089/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA089/1
start: 1843
end: 1886
orientation: inline

L3S2P11 Terminator
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
label: L3S2P11 Terminator
note: L3S2P11 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA089/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA089/annotation1/range/1

https://synbiohub.org/public/SEGA/SEGA089/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA089/1
start: 538
end: 594
orientation: inline

high TIR
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
note: TIR
note: high TIR
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA089/1
role: SO:0000139
location: https://synbiohub.org/public/SEGA/SEGA089/annotation8/range/1

https://synbiohub.org/public/SEGA/SEGA089/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA089/1
start: 3930
end: 3956
orientation: inline

TcR
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
codon start: 1
gene: tet
label: TcR
note: TcR
note: confers resistance to tetracycline
product: tetracycline efflux protein
translation: MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHYGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA089/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA089/annotation14/range/1

https://synbiohub.org/public/SEGA/SEGA089/annotation14/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA089/1
start: 4846
end: 6036
orientation: inline

EM7 promoter
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
label: EM7 promoter
note: EM7 promoter
note: synthetic bacterial promoter
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA089/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA089/annotation3/range/1

https://synbiohub.org/public/SEGA/SEGA089/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA089/1
start: 1783
end: 1830
orientation: reverseComplement

pstS
type: SequenceAnnotation
persistent identity: annotation16
display id: annotation16
version: 1
codon start: 1
label: pstS
note: pstS
translation: MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKWADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA089/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA089/annotation16/range/1

https://synbiohub.org/public/SEGA/SEGA089/annotation16/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA089/1
start: 6282
end: 6529
orientation: inline

L3S3P22 Terminator (synthetic)
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
label: L3S3P22 Terminator (synthetic)
note: L3S3P22 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA089/1
role: SO:0000174
location: https://synbiohub.org/public/SEGA/SEGA089/annotation12/range/1

https://synbiohub.org/public/SEGA/SEGA089/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA089/1
start: 4719
end: 4767
orientation: inline

PROTi-Linker
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
label: PROTi-Linker
note: PROTi-Linker
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA089/1
role: SO:0001411
location: https://synbiohub.org/public/SEGA/SEGA089/annotation10/range/1

https://synbiohub.org/public/SEGA/SEGA089/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA089/1
start: 3969
end: 4004
orientation: inline

Promoter 3BCD2
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
label: Promoter 3BCD2
note: Promoter 3BCD2
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA089/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA089/annotation13/range/1

https://synbiohub.org/public/SEGA/SEGA089/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA089/1
start: 4778
end: 4806
orientation: inline

TEV site
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
codon start: 1
label: TEV site
note: TEV site
product: tobacco etch virus (TEV) protease recognition and cleavage site
translation: MENLYFQ
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA089/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA089/annotation9/range/1

https://synbiohub.org/public/SEGA/SEGA089/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA089/1
start: 3948
end: 3968
orientation: inline

rhaR
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
codon start: 1
gene: E. coli rhaR
label: rhaR
note: needed for rhamnose-dependent activation of the rhaB promoter
note: rhaR
product: transcriptional activator of rhaR and rhaS
translation: MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQGAIPGFNASAGQPHWRLGSMGMAQARQVIGQLEHESSQHVPFANEMAELLFGQLVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRHLNSQKD
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA089/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA089/annotation5/range/1

https://synbiohub.org/public/SEGA/SEGA089/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA089/1
start: 1899
end: 2744
orientation: reverseComplement

glmS
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
codon start: 1
label: glmS
note: glmS
translation: VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA089/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA089/annotation0/range/1

https://synbiohub.org/public/SEGA/SEGA089/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA089/1
start: 1
end: 528
orientation: inline

rhaB promoter
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
label: rhaB promoter
note: promoter of the E. coli rhaBAD operon, conferring tight induction with L-rhamnose and repression with D-glucose in the presence of RhaR and RhaS (Giacalone et al., 2006)
note: rhaB promoter
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA089/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA089/annotation7/range/1

https://synbiohub.org/public/SEGA/SEGA089/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA089/1
start: 3800
end: 3918
orientation: inline

galK
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
EC number: 2.7.1.6
codon start: 1
db xref: ASAP:ABE-0002568
db xref: EcoGene:EG10363
db xref: GI:16128725
db xref: GeneID:945358
db xref: UniProtKB/Swiss-Prot:P0A6T3
function: enzyme; Degradation of small molecules: Carbon compounds
gene: galK
gene synonym: ECK0746; galA; JW0740
label: galK
locus tag: b0757
note: galK
product: galactokinase
protein id: NP_415278.1
translation: MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVLPCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLDAPIVAHENYQWANYVRGVVKHLQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQEAENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTLVGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTVEAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGGGFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA089/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA089/annotation2/range/1

https://synbiohub.org/public/SEGA/SEGA089/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA089/1
start: 605
end: 1753
orientation: reverseComplement

rrnC
type: SequenceAnnotation
persistent identity: annotation15
display id: annotation15
version: 1
label: rrnC
note: rrnC
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA089/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA089/annotation15/range/1

https://synbiohub.org/public/SEGA/SEGA089/annotation15/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA089/1
start: 6047
end: 6087
orientation: inline

https://synbiohub.org/public/SEGA/SEGA049/1
type: ComponentDefinition
persistent identity: SEGA049
display id: SEGA049
version: 1
description: natural linear DNA.
owned by: carbay
owned by: morno
date: 08-SEP-2021
division: UNA
keywords: .
locus: SEGA049
molecule: ds-DNA
organism: unspecified
reference: https://synbiohub.org/user/carbay/SEGA/SEGA049_reference0/1
source: natural DNA sequence
type: DnaRegion
type: SO:0000987
role: SO:0000804
sequence annotation: TEV site
sequence annotation: rhaS
sequence annotation: glmS
sequence annotation: rhaR
sequence annotation: TcR
sequence annotation: L3S2P11 Terminator
sequence annotation: Promoter 3BCD2
sequence annotation: high TIR
sequence annotation: PROTi-Linker
sequence annotation: pstS
sequence annotation: GFP
sequence annotation: L3S3P22 Terminator (synthetic)
sequence annotation: rrnC
sequence annotation: rhaB promoter
sequence: https://synbiohub.org/public/SEGA/SEGA049_seq/1

TEV site
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
codon start: 1
label: TEV site
note: TEV site
product: tobacco etch virus (TEV) protease recognition and cleavage site
translation: MENLYFQ
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA049/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA049/annotation6/range/1

https://synbiohub.org/public/SEGA/SEGA049/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA049/1
start: 2656
end: 2676
orientation: inline

rhaS
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
codon start: 1
gene: E. coli rhaS
label: rhaS
note: activated by L-rhamnose
note: rhaS
product: positive regulator of the rhaB promoter
translation: MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHGTGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLLPQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKSSLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQRYLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGFLQ
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA049/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA049/annotation3/range/1

https://synbiohub.org/public/SEGA/SEGA049/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA049/1
start: 1526
end: 2362
orientation: reverseComplement

glmS
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
codon start: 1
label: glmS
note: glmS
translation: VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA049/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA049/annotation0/range/1

https://synbiohub.org/public/SEGA/SEGA049/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA049/1
start: 1
end: 528
orientation: inline

rhaR
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
codon start: 1
gene: E. coli rhaR
label: rhaR
note: needed for rhamnose-dependent activation of the rhaB promoter
note: rhaR
product: transcriptional activator of rhaR and rhaS
translation: MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQGAIPGFNASAGQPHWRLGSMGMAQARQVIGQLEHESSQHVPFANEMAELLFGQLVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRHLNSQKD
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA049/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA049/annotation2/range/1

https://synbiohub.org/public/SEGA/SEGA049/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA049/1
start: 607
end: 1452
orientation: reverseComplement

TcR
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
codon start: 1
gene: tet
label: TcR
note: TcR
note: confers resistance to tetracycline
product: tetracycline efflux protein
translation: MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHYGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVIFGEDRFRWSATMSGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA049/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA049/annotation11/range/1

https://synbiohub.org/public/SEGA/SEGA049/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA049/1
start: 3554
end: 4744
orientation: inline

L3S2P11 Terminator
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
label: L3S2P11 Terminator
note: L3S2P11 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA049/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA049/annotation1/range/1

https://synbiohub.org/public/SEGA/SEGA049/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA049/1
start: 538
end: 594
orientation: inline

Promoter 3BCD2
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
label: Promoter 3BCD2
note: Promoter 3BCD2
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA049/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA049/annotation10/range/1

https://synbiohub.org/public/SEGA/SEGA049/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA049/1
start: 3486
end: 3514
orientation: inline

high TIR
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
note: TIR
note: high TIR
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA049/1
role: SO:0000139
location: https://synbiohub.org/public/SEGA/SEGA049/annotation5/range/1

https://synbiohub.org/public/SEGA/SEGA049/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA049/1
start: 2638
end: 2664
orientation: inline

PROTi-Linker
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
label: PROTi-Linker
note: PROTi-Linker
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA049/1
role: SO:0001411
location: https://synbiohub.org/public/SEGA/SEGA049/annotation7/range/1

https://synbiohub.org/public/SEGA/SEGA049/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA049/1
start: 2677
end: 2712
orientation: inline

pstS
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
codon start: 1
label: pstS
note: pstS
translation: MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKWADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA049/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA049/annotation13/range/1

https://synbiohub.org/public/SEGA/SEGA049/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA049/1
start: 4990
end: 5237
orientation: inline

GFP
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
codon start: 1
label: GFP
note: GFP
product: green fluorescent protein
translation: SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYK
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA049/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA049/annotation8/range/1

https://synbiohub.org/public/SEGA/SEGA049/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA049/1
start: 2713
end: 3423
orientation: inline

L3S3P22 Terminator (synthetic)
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
label: L3S3P22 Terminator (synthetic)
note: L3S3P22 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA049/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA049/annotation9/range/1

https://synbiohub.org/public/SEGA/SEGA049/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA049/1
start: 3427
end: 3475
orientation: inline

rrnC
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
label: rrnC
note: rrnC
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA049/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA049/annotation12/range/1

https://synbiohub.org/public/SEGA/SEGA049/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA049/1
start: 4755
end: 4795
orientation: inline

rhaB promoter
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
label: rhaB promoter
note: promoter of the E. coli rhaBAD operon, conferring tight induction with L-rhamnose and repression with D-glucose in the presence of RhaR and RhaS (Giacalone et al., 2006)
note: rhaB promoter
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA049/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA049/annotation4/range/1

https://synbiohub.org/public/SEGA/SEGA049/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA049/1
start: 2508
end: 2626
orientation: inline

https://synbiohub.org/public/SEGA/SEGA087/1
type: ComponentDefinition
persistent identity: SEGA087
display id: SEGA087
version: 1
description: natural linear DNA.
owned by: carbay
owned by: morno
date: 09-SEP-2021
division: UNA
keywords: .
locus: SEGA087
molecule: ds-DNA
organism: unspecified
reference: https://synbiohub.org/user/carbay/SEGA/SEGA087_reference0/1
source: natural DNA sequence
type: DnaRegion
type: SO:0000987
role: SO:0000804
sequence annotation: pstS
sequence annotation: Promoter 3BCD2
sequence annotation: rhaB promoter
sequence annotation: galK
sequence annotation: PROTi-Linker
sequence annotation: rrnC
sequence annotation: low TIR
sequence annotation: TcR
sequence annotation: rhaR
sequence annotation: glmS
sequence annotation: Terminator rrnB T1
sequence annotation: L3S3P22 Terminator (synthetic)
sequence annotation: L3S2P11 Terminator
sequence annotation: EM7 promoter
sequence annotation: GFP
sequence annotation: rhaS
sequence annotation: TEV site
sequence: https://synbiohub.org/public/SEGA/SEGA087_seq/1

pstS
type: SequenceAnnotation
persistent identity: annotation16
display id: annotation16
version: 1
codon start: 1
label: pstS
note: pstS
translation: MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKWADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA087/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA087/annotation16/range/1

https://synbiohub.org/public/SEGA/SEGA087/annotation16/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA087/1
start: 6282
end: 6529
orientation: inline

Promoter 3BCD2
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
label: Promoter 3BCD2
note: Promoter 3BCD2
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA087/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA087/annotation13/range/1

https://synbiohub.org/public/SEGA/SEGA087/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA087/1
start: 4778
end: 4806
orientation: inline

rhaB promoter
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
label: rhaB promoter
note: promoter of the E. coli rhaBAD operon, conferring tight induction with L-rhamnose and repression with D-glucose in the presence of RhaR and RhaS (Giacalone et al., 2006)
note: rhaB promoter
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA087/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA087/annotation7/range/1

https://synbiohub.org/public/SEGA/SEGA087/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA087/1
start: 3800
end: 3918
orientation: inline

galK
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
EC number: 2.7.1.6
codon start: 1
db xref: ASAP:ABE-0002568
db xref: EcoGene:EG10363
db xref: GI:16128725
db xref: GeneID:945358
db xref: UniProtKB/Swiss-Prot:P0A6T3
function: enzyme; Degradation of small molecules: Carbon compounds
gene: galK
gene synonym: ECK0746; galA; JW0740
label: galK
locus tag: b0757
note: galK
product: galactokinase
protein id: NP_415278.1
translation: MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVLPCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLDAPIVAHENYQWANYVRGVVKHLQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQEAENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTLVGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTVEAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGGGFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA087/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA087/annotation2/range/1

https://synbiohub.org/public/SEGA/SEGA087/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA087/1
start: 605
end: 1753
orientation: reverseComplement

PROTi-Linker
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
label: PROTi-Linker
note: PROTi-Linker
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA087/1
role: SO:0001411
location: https://synbiohub.org/public/SEGA/SEGA087/annotation10/range/1

https://synbiohub.org/public/SEGA/SEGA087/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA087/1
start: 3969
end: 4004
orientation: inline

rrnC
type: SequenceAnnotation
persistent identity: annotation15
display id: annotation15
version: 1
label: rrnC
note: rrnC
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA087/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA087/annotation15/range/1

https://synbiohub.org/public/SEGA/SEGA087/annotation15/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA087/1
start: 6047
end: 6087
orientation: inline

low TIR
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
note: TIR
note: low TIR
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA087/1
role: SO:0000139
location: https://synbiohub.org/public/SEGA/SEGA087/annotation8/range/1

https://synbiohub.org/public/SEGA/SEGA087/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA087/1
start: 3930
end: 3956
orientation: inline

TcR
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
codon start: 1
gene: tet
label: TcR
note: TcR
note: confers resistance to tetracycline
product: tetracycline efflux protein
translation: MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHYGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA087/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA087/annotation14/range/1

https://synbiohub.org/public/SEGA/SEGA087/annotation14/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA087/1
start: 4846
end: 6036
orientation: inline

rhaR
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
codon start: 1
gene: E. coli rhaR
label: rhaR
note: needed for rhamnose-dependent activation of the rhaB promoter
note: rhaR
product: transcriptional activator of rhaR and rhaS
translation: MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQGAIPGFNASAGQPHWRLGSMGMAQARQVIGQLEHESSQHVPFANEMAELLFGQLVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRHLNSQKD
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA087/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA087/annotation5/range/1

https://synbiohub.org/public/SEGA/SEGA087/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA087/1
start: 1899
end: 2744
orientation: reverseComplement

glmS
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
codon start: 1
label: glmS
note: glmS
translation: VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA087/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA087/annotation0/range/1

https://synbiohub.org/public/SEGA/SEGA087/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA087/1
start: 1
end: 528
orientation: inline

Terminator rrnB T1
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
label: Terminator rrnB T1
note: Terminator rrnB T1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA087/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA087/annotation4/range/1

https://synbiohub.org/public/SEGA/SEGA087/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA087/1
start: 1843
end: 1886
orientation: inline

L3S3P22 Terminator (synthetic)
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
label: L3S3P22 Terminator (synthetic)
note: L3S3P22 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA087/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA087/annotation12/range/1

https://synbiohub.org/public/SEGA/SEGA087/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA087/1
start: 4719
end: 4767
orientation: inline

L3S2P11 Terminator
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
label: L3S2P11 Terminator
note: L3S2P11 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA087/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA087/annotation1/range/1

https://synbiohub.org/public/SEGA/SEGA087/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA087/1
start: 538
end: 594
orientation: inline

EM7 promoter
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
label: EM7 promoter
note: EM7 promoter
note: synthetic bacterial promoter
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA087/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA087/annotation3/range/1

https://synbiohub.org/public/SEGA/SEGA087/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA087/1
start: 1783
end: 1830
orientation: reverseComplement

GFP
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
codon start: 1
label: GFP
note: GFP
product: green fluorescent protein
translation: SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYK
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA087/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA087/annotation11/range/1

https://synbiohub.org/public/SEGA/SEGA087/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA087/1
start: 4005
end: 4715
orientation: inline

rhaS
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
codon start: 1
gene: E. coli rhaS
label: rhaS
note: activated by L-rhamnose
note: rhaS
product: positive regulator of the rhaB promoter
translation: MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHGTGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLLPQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKSSLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQRYLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGFLQ
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA087/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA087/annotation6/range/1

https://synbiohub.org/public/SEGA/SEGA087/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA087/1
start: 2818
end: 3654
orientation: reverseComplement

TEV site
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
codon start: 1
label: TEV site
note: TEV site
product: tobacco etch virus (TEV) protease recognition and cleavage site
translation: MENLYFQ
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA087/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA087/annotation9/range/1

https://synbiohub.org/public/SEGA/SEGA087/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA087/1
start: 3948
end: 3968
orientation: inline

https://synbiohub.org/public/SEGA/SEGA051/1
type: ComponentDefinition
persistent identity: SEGA051
display id: SEGA051
version: 1
description: natural linear DNA.
owned by: carbay
owned by: morno
date: 08-SEP-2021
division: UNA
keywords: .
locus: SEGA051
molecule: ds-DNA
organism: unspecified
reference: https://synbiohub.org/user/carbay/SEGA/SEGA051_reference0/1
source: natural DNA sequence
type: DnaRegion
type: SO:0000987
role: SO:0000804
sequence annotation: sfGFP
sequence annotation: medium TIR
sequence annotation: rrnC
sequence annotation: rhaS
sequence annotation: pelB signal sequence
sequence annotation: pstS
sequence annotation: rhaR
sequence annotation: rhaB promoter
sequence annotation: TcR
sequence annotation: L3S2P11 Terminator
sequence annotation: Promoter 3BCD2
sequence annotation: glmS
sequence annotation: L3S3P22 Terminator (synthetic)
sequence: https://synbiohub.org/public/SEGA/SEGA051_seq/1

sfGFP
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
codon start: 1
label: sfGFP
note: sfGFP
product: superfolder GFP
translation: RKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDGTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEFVTAAGITHGMDELYK
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA051/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA051/annotation7/range/1

https://synbiohub.org/public/SEGA/SEGA051/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA051/1
start: 2722
end: 3432
orientation: inline

medium TIR
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
note: TIR
note: medium TIR
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA051/1
role: SO:0000139
location: https://synbiohub.org/public/SEGA/SEGA051/annotation5/range/1

https://synbiohub.org/public/SEGA/SEGA051/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA051/1
start: 2638
end: 2664
orientation: inline

rrnC
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
label: rrnC
note: rrnC
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA051/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA051/annotation11/range/1

https://synbiohub.org/public/SEGA/SEGA051/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA051/1
start: 4764
end: 4804
orientation: inline

rhaS
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
codon start: 1
gene: E. coli rhaS
label: rhaS
note: activated by L-rhamnose
note: rhaS
product: positive regulator of the rhaB promoter
translation: MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHGTGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLLPQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKSSLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQRYLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGFLQ
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA051/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA051/annotation3/range/1

https://synbiohub.org/public/SEGA/SEGA051/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA051/1
start: 1526
end: 2362
orientation: reverseComplement

pelB signal sequence
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
codon start: 1
gene: pelB (fragment)
label: pelB signal sequence
note: pelB signal sequence
product: leader peptide for secretion
translation: MKYLLPTAAAGLLLLAAQPAMA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA051/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA051/annotation6/range/1

https://synbiohub.org/public/SEGA/SEGA051/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA051/1
start: 2656
end: 2721
orientation: inline

pstS
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
codon start: 1
label: pstS
note: pstS
translation: MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKWADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA051/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA051/annotation12/range/1

https://synbiohub.org/public/SEGA/SEGA051/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA051/1
start: 4999
end: 5246
orientation: inline

rhaR
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
codon start: 1
gene: E. coli rhaR
label: rhaR
note: needed for rhamnose-dependent activation of the rhaB promoter
note: rhaR
product: transcriptional activator of rhaR and rhaS
translation: MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQGAIPGFNASAGQPHWRLGSMGMAQARQVIGQLEHESSQHVPFANEMAELLFGQLVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRHLNSQKD
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA051/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA051/annotation2/range/1

https://synbiohub.org/public/SEGA/SEGA051/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA051/1
start: 607
end: 1452
orientation: reverseComplement

rhaB promoter
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
label: rhaB promoter
note: promoter of the E. coli rhaBAD operon, conferring tight induction with L-rhamnose and repression with D-glucose in the presence of RhaR and RhaS (Giacalone et al., 2006)
note: rhaB promoter
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA051/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA051/annotation4/range/1

https://synbiohub.org/public/SEGA/SEGA051/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA051/1
start: 2508
end: 2626
orientation: inline

TcR
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
codon start: 1
gene: tet
label: TcR
note: TcR
note: confers resistance to tetracycline
product: tetracycline efflux protein
translation: MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHYGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVIFGEDRFRWSATMSGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA051/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA051/annotation10/range/1

https://synbiohub.org/public/SEGA/SEGA051/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA051/1
start: 3563
end: 4753
orientation: inline

L3S2P11 Terminator
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
label: L3S2P11 Terminator
note: L3S2P11 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA051/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA051/annotation1/range/1

https://synbiohub.org/public/SEGA/SEGA051/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA051/1
start: 538
end: 594
orientation: inline

Promoter 3BCD2
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
label: Promoter 3BCD2
note: Promoter 3BCD2
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA051/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA051/annotation9/range/1

https://synbiohub.org/public/SEGA/SEGA051/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA051/1
start: 3495
end: 3523
orientation: inline

glmS
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
codon start: 1
label: glmS
note: glmS
translation: VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA051/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA051/annotation0/range/1

https://synbiohub.org/public/SEGA/SEGA051/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA051/1
start: 1
end: 528
orientation: inline

L3S3P22 Terminator (synthetic)
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
label: L3S3P22 Terminator (synthetic)
note: L3S3P22 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA051/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA051/annotation8/range/1

https://synbiohub.org/public/SEGA/SEGA051/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA051/1
start: 3436
end: 3484
orientation: inline

https://synbiohub.org/public/SEGA/SEGA012/1
type: ComponentDefinition
persistent identity: SEGA012
display id: SEGA012
version: 1
description: natural linear DNA.
owned by: carbay
owned by: morno
date: 09-SEP-2021
division: UNA
keywords: .
locus: SEGA012
molecule: ds-DNA
organism: unspecified
reference: https://synbiohub.org/public/SEGA/reference0_1/1
source: natural DNA sequence
type: DnaRegion
type: SO:0000987
role: SO:0000804
sequence annotation: P-J23100
sequence annotation: rrnC
sequence annotation: TcR
sequence annotation: medium TIR
sequence annotation: Promoter 3BCD2
sequence annotation: GFP
sequence annotation: L3S2P11 Terminator
sequence annotation: TEV site
sequence annotation: pstS
sequence annotation: glmS
sequence annotation: L3S3P22 Terminator (synthetic)
sequence: https://synbiohub.org/public/SEGA/SEGA012_seq/1

P-J23100
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
label: P-J23100
note: P-J23100
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA012/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA012/annotation2/range/1

https://synbiohub.org/public/SEGA/SEGA012/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA012/1
start: 604
end: 638
orientation: inline

rrnC
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
label: rrnC
note: rrnC
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA012/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA012/annotation9/range/1

https://synbiohub.org/public/SEGA/SEGA012/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA012/1
start: 2731
end: 2771
orientation: inline

TcR
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
codon start: 1
gene: tet
label: TcR
note: TcR
note: confers resistance to tetracycline
product: tetracycline efflux protein
translation: MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHYGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVIFGEDRFRWSATMSGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA012/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA012/annotation8/range/1

https://synbiohub.org/public/SEGA/SEGA012/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA012/1
start: 1530
end: 2720
orientation: inline

medium TIR
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
note: TIR
note: medium TIR
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA012/1
role: SO:0000139
location: https://synbiohub.org/public/SEGA/SEGA012/annotation3/range/1

https://synbiohub.org/public/SEGA/SEGA012/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA012/1
start: 650
end: 676
orientation: inline

Promoter 3BCD2
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
label: Promoter 3BCD2
note: Promoter 3BCD2
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA012/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA012/annotation7/range/1

https://synbiohub.org/public/SEGA/SEGA012/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA012/1
start: 1462
end: 1490
orientation: inline

GFP
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
codon start: 1
label: GFP
note: GFP
product: green fluorescent protein
translation: SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYK
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA012/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA012/annotation5/range/1

https://synbiohub.org/public/SEGA/SEGA012/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA012/1
start: 689
end: 1399
orientation: inline

L3S2P11 Terminator
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
label: L3S2P11 Terminator
note: L3S2P11 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA012/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA012/annotation1/range/1

https://synbiohub.org/public/SEGA/SEGA012/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA012/1
start: 538
end: 594
orientation: inline

TEV site
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
codon start: 1
label: TEV site
note: TEV site
product: tobacco etch virus (TEV) protease recognition and cleavage site
translation: MENLYFQ
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA012/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA012/annotation4/range/1

https://synbiohub.org/public/SEGA/SEGA012/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA012/1
start: 668
end: 688
orientation: inline

pstS
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
codon start: 1
label: pstS
note: pstS
translation: MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKWADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA012/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA012/annotation10/range/1

https://synbiohub.org/public/SEGA/SEGA012/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA012/1
start: 2966
end: 3213
orientation: inline

glmS
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
codon start: 1
label: glmS
note: glmS
translation: VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA012/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA012/annotation0/range/1

https://synbiohub.org/public/SEGA/SEGA012/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA012/1
start: 1
end: 528
orientation: inline

L3S3P22 Terminator (synthetic)
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
label: L3S3P22 Terminator (synthetic)
note: L3S3P22 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA012/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA012/annotation6/range/1

https://synbiohub.org/public/SEGA/SEGA012/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA012/1
start: 1403
end: 1451
orientation: inline

https://synbiohub.org/public/SEGA/SEGA096/1
type: ComponentDefinition
persistent identity: SEGA096
display id: SEGA096
version: 1
description: natural linear DNA.
owned by: carbay
owned by: morno
date: 09-SEP-2021
division: UNA
keywords: .
locus: SEGA096
molecule: ds-DNA
organism: unspecified
reference: https://synbiohub.org/user/carbay/SEGA/SEGA096_reference0/1
source: natural DNA sequence
type: DnaRegion
type: SO:0000987
role: SO:0000804
sequence annotation: pstS
sequence annotation: P-J23100
sequence annotation: glmS
sequence annotation: TcR
sequence annotation: low TIR
sequence annotation: rrnC
sequence annotation: BCD
sequence annotation: L3S2P11 Terminator
sequence: https://synbiohub.org/public/SEGA/SEGA096_seq/1

pstS
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
codon start: 1
label: pstS
note: pstS
translation: MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKWADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA096/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA096/annotation7/range/1

https://synbiohub.org/public/SEGA/SEGA096/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA096/1
start: 2082
end: 2329
orientation: inline

P-J23100
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
label: P-J23100
note: P-J23100
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA096/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA096/annotation2/range/1

https://synbiohub.org/public/SEGA/SEGA096/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA096/1
start: 604
end: 638
orientation: inline

glmS
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
codon start: 1
label: glmS
note: glmS
translation: VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA096/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA096/annotation0/range/1

https://synbiohub.org/public/SEGA/SEGA096/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA096/1
start: 1
end: 528
orientation: inline

TcR
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
codon start: 1
gene: tet
label: TcR
note: TcR
note: confers resistance to tetracycline
product: tetracycline efflux protein
translation: VLPGLLRDIVHSDSIASHYGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVIFGEDRFRWSATMSGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA096/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA096/annotation5/range/1

https://synbiohub.org/public/SEGA/SEGA096/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA096/1
start: 724
end: 1836
orientation: inline

low TIR
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
note: TIR
note: low TIR
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA096/1
role: SO:0000139
location: https://synbiohub.org/public/SEGA/SEGA096/annotation3/range/1

https://synbiohub.org/public/SEGA/SEGA096/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA096/1
start: 650
end: 676
orientation: inline

rrnC
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
label: rrnC
note: rrnC
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA096/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA096/annotation6/range/1

https://synbiohub.org/public/SEGA/SEGA096/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA096/1
start: 1847
end: 1887
orientation: inline

BCD
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
codon start: 1
label: BCD
note: BCD
translation: MKAIFVLKHLNHAKEVF
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA096/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA096/annotation4/range/1

https://synbiohub.org/public/SEGA/SEGA096/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA096/1
start: 668
end: 723
orientation: inline

L3S2P11 Terminator
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
label: L3S2P11 Terminator
note: L3S2P11 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA096/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA096/annotation1/range/1

https://synbiohub.org/public/SEGA/SEGA096/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA096/1
start: 538
end: 594
orientation: inline

https://synbiohub.org/public/SEGA/SEGA047/1
type: ComponentDefinition
persistent identity: SEGA047
display id: SEGA047
version: 1
description: natural linear DNA.
owned by: carbay
owned by: morno
date: 08-SEP-2021
division: UNA
keywords: .
locus: SEGA047
molecule: ds-DNA
organism: unspecified
reference: https://synbiohub.org/user/carbay/SEGA/SEGA047_reference0/1
source: natural DNA sequence
type: DnaRegion
type: SO:0000987
role: SO:0000804
sequence annotation: rhaB promoter
sequence annotation: PROTi-Linker
sequence annotation: Promoter 3BCD2
sequence annotation: pstS
sequence annotation: low TIR
sequence annotation: L3S3P22 Terminator (synthetic)
sequence annotation: rrnC
sequence annotation: TcR
sequence annotation: glmS
sequence annotation: rhaS
sequence annotation: GFP
sequence annotation: L3S2P11 Terminator
sequence annotation: rhaR
sequence annotation: TEV site
sequence: https://synbiohub.org/public/SEGA/SEGA047_seq/1

rhaB promoter
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
label: rhaB promoter
note: promoter of the E. coli rhaBAD operon, conferring tight induction with L-rhamnose and repression with D-glucose in the presence of RhaR and RhaS (Giacalone et al., 2006)
note: rhaB promoter
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA047/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA047/annotation4/range/1

https://synbiohub.org/public/SEGA/SEGA047/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA047/1
start: 2508
end: 2626
orientation: inline

PROTi-Linker
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
label: PROTi-Linker
note: PROTi-Linker
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA047/1
role: SO:0001411
location: https://synbiohub.org/public/SEGA/SEGA047/annotation7/range/1

https://synbiohub.org/public/SEGA/SEGA047/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA047/1
start: 2677
end: 2712
orientation: inline

Promoter 3BCD2
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
label: Promoter 3BCD2
note: Promoter 3BCD2
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA047/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA047/annotation10/range/1

https://synbiohub.org/public/SEGA/SEGA047/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA047/1
start: 3486
end: 3514
orientation: inline

pstS
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
codon start: 1
label: pstS
note: pstS
translation: MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKWADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA047/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA047/annotation13/range/1

https://synbiohub.org/public/SEGA/SEGA047/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA047/1
start: 4990
end: 5237
orientation: inline

low TIR
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
note: TIR
note: low TIR
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA047/1
role: SO:0000139
location: https://synbiohub.org/public/SEGA/SEGA047/annotation5/range/1

https://synbiohub.org/public/SEGA/SEGA047/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA047/1
start: 2638
end: 2664
orientation: inline

L3S3P22 Terminator (synthetic)
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
label: L3S3P22 Terminator (synthetic)
note: L3S3P22 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA047/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA047/annotation9/range/1

https://synbiohub.org/public/SEGA/SEGA047/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA047/1
start: 3427
end: 3475
orientation: inline

rrnC
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
label: rrnC
note: rrnC
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA047/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA047/annotation12/range/1

https://synbiohub.org/public/SEGA/SEGA047/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA047/1
start: 4755
end: 4795
orientation: inline

TcR
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
codon start: 1
gene: tet
label: TcR
note: TcR
note: confers resistance to tetracycline
product: tetracycline efflux protein
translation: MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHYGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVIFGEDRFRWSATMSGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA047/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA047/annotation11/range/1

https://synbiohub.org/public/SEGA/SEGA047/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA047/1
start: 3554
end: 4744
orientation: inline

glmS
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
codon start: 1
label: glmS
note: glmS
translation: VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA047/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA047/annotation0/range/1

https://synbiohub.org/public/SEGA/SEGA047/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA047/1
start: 1
end: 528
orientation: inline

rhaS
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
codon start: 1
gene: E. coli rhaS
label: rhaS
note: activated by L-rhamnose
note: rhaS
product: positive regulator of the rhaB promoter
translation: MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHGTGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLLPQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKSSLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQRYLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGFLQ
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA047/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA047/annotation3/range/1

https://synbiohub.org/public/SEGA/SEGA047/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA047/1
start: 1526
end: 2362
orientation: reverseComplement

GFP
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
codon start: 1
label: GFP
note: GFP
product: green fluorescent protein
translation: SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYK
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA047/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA047/annotation8/range/1

https://synbiohub.org/public/SEGA/SEGA047/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA047/1
start: 2713
end: 3423
orientation: inline

L3S2P11 Terminator
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
label: L3S2P11 Terminator
note: L3S2P11 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA047/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA047/annotation1/range/1

https://synbiohub.org/public/SEGA/SEGA047/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA047/1
start: 538
end: 594
orientation: inline

rhaR
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
codon start: 1
gene: E. coli rhaR
label: rhaR
note: needed for rhamnose-dependent activation of the rhaB promoter
note: rhaR
product: transcriptional activator of rhaR and rhaS
translation: MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQGAIPGFNASAGQPHWRLGSMGMAQARQVIGQLEHESSQHVPFANEMAELLFGQLVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRHLNSQKD
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA047/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA047/annotation2/range/1

https://synbiohub.org/public/SEGA/SEGA047/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA047/1
start: 607
end: 1452
orientation: reverseComplement

TEV site
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
codon start: 1
label: TEV site
note: TEV site
product: tobacco etch virus (TEV) protease recognition and cleavage site
translation: MENLYFQ
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA047/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA047/annotation6/range/1

https://synbiohub.org/public/SEGA/SEGA047/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA047/1
start: 2656
end: 2676
orientation: inline

https://synbiohub.org/public/SEGA/SEGA054/1
type: ComponentDefinition
persistent identity: SEGA054
display id: SEGA054
version: 1
description: natural linear DNA.
owned by: carbay
owned by: morno
date: 08-SEP-2021
division: UNA
keywords: .
locus: SEGA054
molecule: ds-DNA
organism: unspecified
reference: https://synbiohub.org/user/carbay/SEGA/SEGA054_reference0/1
source: natural DNA sequence
type: DnaRegion
type: SO:0000987
role: SO:0000804
sequence annotation: L3S2P11 Terminator
sequence annotation: YebF
sequence annotation: sfGFP
sequence annotation: rhaB promoter
sequence annotation: Promoter 3BCD2
sequence annotation: yebF
sequence annotation: medium TIR
sequence annotation: rrnC
sequence annotation: glmS
sequence annotation: rhaS
sequence annotation: pstS
sequence annotation: rhaR
sequence annotation: L3S3P22 Terminator (synthetic)
sequence annotation: TcR
sequence: https://synbiohub.org/public/SEGA/SEGA054_seq/1

L3S2P11 Terminator
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
label: L3S2P11 Terminator
note: L3S2P11 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA054/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA054/annotation1/range/1

https://synbiohub.org/public/SEGA/SEGA054/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA054/1
start: 538
end: 594
orientation: inline

YebF
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
codon start: 1
label: YebF
note: YebF
product: full size yebF protein
translation: ANNETSKSVTFPKCEDLDAAGIAASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHYQVSVDCKAGMAEYQRR
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA054/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA054/annotation7/range/1

https://synbiohub.org/public/SEGA/SEGA054/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA054/1
start: 2719
end: 3009
orientation: inline

sfGFP
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
codon start: 1
label: sfGFP
note: sfGFP
product: superfolder GFP
translation: RKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDGTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEFVTAAGITHGMDELYK
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA054/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA054/annotation8/range/1

https://synbiohub.org/public/SEGA/SEGA054/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA054/1
start: 3010
end: 3720
orientation: inline

rhaB promoter
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
label: rhaB promoter
note: promoter of the E. coli rhaBAD operon, conferring tight induction with L-rhamnose and repression with D-glucose in the presence of RhaR and RhaS (Giacalone et al., 2006)
note: rhaB promoter
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA054/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA054/annotation4/range/1

https://synbiohub.org/public/SEGA/SEGA054/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA054/1
start: 2508
end: 2626
orientation: inline

Promoter 3BCD2
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
label: Promoter 3BCD2
note: Promoter 3BCD2
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA054/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA054/annotation10/range/1

https://synbiohub.org/public/SEGA/SEGA054/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA054/1
start: 3783
end: 3811
orientation: inline

yebF
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
codon start: 1
db xref: ASAP:ABE-0006141
db xref: EcoGene:EG11807
db xref: GI:145698278
db xref: GeneID:946363
db xref: UniProtKB/Swiss-Prot:P33219
gene: yebF
gene synonym: ECK1848; JW1836
label: yebF
locus tag: b1847
note: yebFss
product: yebF signal sequence
protein id: NP_416361.2
translation: MKKRGAFLGLLLVSACASVFA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA054/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA054/annotation6/range/1

https://synbiohub.org/public/SEGA/SEGA054/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA054/1
start: 2656
end: 2718
orientation: inline

medium TIR
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
note: TIR
note: medium TIR
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA054/1
role: SO:0000139
location: https://synbiohub.org/public/SEGA/SEGA054/annotation5/range/1

https://synbiohub.org/public/SEGA/SEGA054/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA054/1
start: 2638
end: 2664
orientation: inline

rrnC
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
label: rrnC
note: rrnC
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA054/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA054/annotation12/range/1

https://synbiohub.org/public/SEGA/SEGA054/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA054/1
start: 5052
end: 5092
orientation: inline

glmS
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
codon start: 1
label: glmS
note: glmS
translation: VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA054/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA054/annotation0/range/1

https://synbiohub.org/public/SEGA/SEGA054/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA054/1
start: 1
end: 528
orientation: inline

rhaS
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
codon start: 1
gene: E. coli rhaS
label: rhaS
note: activated by L-rhamnose
note: rhaS
product: positive regulator of the rhaB promoter
translation: MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHGTGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLLPQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKSSLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQRYLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGFLQ
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA054/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA054/annotation3/range/1

https://synbiohub.org/public/SEGA/SEGA054/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA054/1
start: 1526
end: 2362
orientation: reverseComplement

pstS
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
codon start: 1
label: pstS
note: pstS
translation: MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKWADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA054/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA054/annotation13/range/1

https://synbiohub.org/public/SEGA/SEGA054/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA054/1
start: 5287
end: 5534
orientation: inline

rhaR
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
codon start: 1
gene: E. coli rhaR
label: rhaR
note: needed for rhamnose-dependent activation of the rhaB promoter
note: rhaR
product: transcriptional activator of rhaR and rhaS
translation: MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQGAIPGFNASAGQPHWRLGSMGMAQARQVIGQLEHESSQHVPFANEMAELLFGQLVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRHLNSQKD
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA054/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA054/annotation2/range/1

https://synbiohub.org/public/SEGA/SEGA054/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA054/1
start: 607
end: 1452
orientation: reverseComplement

L3S3P22 Terminator (synthetic)
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
label: L3S3P22 Terminator (synthetic)
note: L3S3P22 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA054/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA054/annotation9/range/1

https://synbiohub.org/public/SEGA/SEGA054/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA054/1
start: 3724
end: 3772
orientation: inline

TcR
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
codon start: 1
gene: tet
label: TcR
note: TcR
note: confers resistance to tetracycline
product: tetracycline efflux protein
translation: MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHYGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVIFGEDRFRWSATMSGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA054/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA054/annotation11/range/1

https://synbiohub.org/public/SEGA/SEGA054/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA054/1
start: 3851
end: 5041
orientation: inline

https://synbiohub.org/public/SEGA/SEGA071/1
type: ComponentDefinition
persistent identity: SEGA071
display id: SEGA071
version: 1
description: natural linear DNA.
owned by: carbay
owned by: morno
date: 08-SEP-2021
division: UNA
keywords: .
locus: SEGA071
molecule: ds-DNA
organism: unspecified
reference: https://synbiohub.org/public/SEGA/reference0_1/1
source: natural DNA sequence
type: DnaRegion
type: SO:0000987
role: SO:0000804
sequence annotation: pstS
sequence annotation: lacI promoter
sequence annotation: TcR
sequence annotation: L3S3P22 Terminator (synthetic)
sequence annotation: rrnC
sequence annotation: L3S2P11 Terminator
sequence annotation: YebF
sequence annotation: yebF
sequence annotation: T7 promoter
sequence annotation: lac operator
sequence annotation: Promoter 3BCD2
sequence annotation: sfGFP
sequence annotation: lacI
sequence annotation: low TIR
sequence annotation: glmS
sequence: https://synbiohub.org/public/SEGA/SEGA071_seq/1

pstS
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
codon start: 1
label: pstS
note: pstS
translation: MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKWADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA071/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA071/annotation14/range/1

https://synbiohub.org/public/SEGA/SEGA071/annotation14/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA071/1
start: 4499
end: 4746
orientation: inline

lacI promoter
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
gene: lacI
label: lacI promoter
note: lacI promoter
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA071/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA071/annotation3/range/1

https://synbiohub.org/public/SEGA/SEGA071/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA071/1
start: 1687
end: 1764
orientation: reverseComplement

TcR
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
codon start: 1
gene: tet
label: TcR
note: TcR
note: confers resistance to tetracycline
product: tetracycline efflux protein
translation: MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHYGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVIFGEDRFRWSATMSGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA071/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA071/annotation12/range/1

https://synbiohub.org/public/SEGA/SEGA071/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA071/1
start: 3063
end: 4253
orientation: inline

L3S3P22 Terminator (synthetic)
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
label: L3S3P22 Terminator (synthetic)
note: L3S3P22 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA071/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA071/annotation10/range/1

https://synbiohub.org/public/SEGA/SEGA071/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA071/1
start: 2936
end: 2984
orientation: inline

rrnC
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
label: rrnC
note: rrnC
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA071/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA071/annotation13/range/1

https://synbiohub.org/public/SEGA/SEGA071/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA071/1
start: 4264
end: 4304
orientation: inline

L3S2P11 Terminator
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
label: L3S2P11 Terminator
note: L3S2P11 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA071/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA071/annotation1/range/1

https://synbiohub.org/public/SEGA/SEGA071/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA071/1
start: 538
end: 594
orientation: inline

YebF
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
codon start: 1
label: YebF
note: YebF
product: full size yebF protein
translation: ANNETSKSVTFPKCEDLDAAGIAASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHYQVSVDCKAGMAEYQRR
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA071/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA071/annotation8/range/1

https://synbiohub.org/public/SEGA/SEGA071/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA071/1
start: 1931
end: 2221
orientation: inline

yebF
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
codon start: 1
db xref: ASAP:ABE-0006141
db xref: EcoGene:EG11807
db xref: GI:145698278
db xref: GeneID:946363
db xref: UniProtKB/Swiss-Prot:P33219
gene: yebF
gene synonym: ECK1848; JW1836
label: yebF
locus tag: b1847
note: yebFss
product: yebF signal sequence
protein id: NP_416361.2
translation: MKKRGAFLGLLLVSACASVFA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA071/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA071/annotation7/range/1

https://synbiohub.org/public/SEGA/SEGA071/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA071/1
start: 1868
end: 1930
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
label: T7 promoter
note: T7 promoter
note: promoter for bacteriophage T7 RNA polymerase
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA071/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA071/annotation4/range/1

https://synbiohub.org/public/SEGA/SEGA071/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA071/1
start: 1795
end: 1813
orientation: inline

lac operator
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
bound moiety: lac repressor encoded by lacI
feature type: protein_bind
label: lac operator
note: The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG).
note: lac operator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA071/1
role: SO:0000110
location: https://synbiohub.org/public/SEGA/SEGA071/annotation5/range/1

https://synbiohub.org/public/SEGA/SEGA071/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA071/1
start: 1814
end: 1838
orientation: inline

Promoter 3BCD2
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
label: Promoter 3BCD2
note: Promoter 3BCD2
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA071/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA071/annotation11/range/1

https://synbiohub.org/public/SEGA/SEGA071/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA071/1
start: 2995
end: 3023
orientation: inline

sfGFP
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
codon start: 1
label: sfGFP
note: sfGFP
product: superfolder GFP
translation: RKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDGTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEFVTAAGITHGMDELYK
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA071/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA071/annotation9/range/1

https://synbiohub.org/public/SEGA/SEGA071/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA071/1
start: 2222
end: 2932
orientation: inline

lacI
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
codon start: 1
gene: lacI
label: lacI
note: The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG).
note: lacI
product: lac repressor
translation: MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA071/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA071/annotation2/range/1

https://synbiohub.org/public/SEGA/SEGA071/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA071/1
start: 604
end: 1686
orientation: reverseComplement

low TIR
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
note: TIR
note: low TIR
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA071/1
role: SO:0000139
location: https://synbiohub.org/public/SEGA/SEGA071/annotation6/range/1

https://synbiohub.org/public/SEGA/SEGA071/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA071/1
start: 1850
end: 1876
orientation: inline

glmS
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
codon start: 1
label: glmS
note: glmS
translation: VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA071/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA071/annotation0/range/1

https://synbiohub.org/public/SEGA/SEGA071/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA071/1
start: 1
end: 528
orientation: inline

https://synbiohub.org/public/SEGA/SEGA091/1
type: ComponentDefinition
persistent identity: SEGA091
display id: SEGA091
version: 1
description: natural linear DNA.
owned by: carbay
owned by: morno
date: 09-SEP-2021
division: UNA
keywords: .
locus: SEGA091
molecule: ds-DNA
organism: unspecified
reference: https://synbiohub.org/user/carbay/SEGA/SEGA091_reference0/1
source: natural DNA sequence
type: DnaRegion
type: SO:0000987
role: SO:0000804
sequence annotation: galK
sequence annotation: rhaB promoter
sequence annotation: medium TIR
sequence annotation: Promoter 3BCD2
sequence annotation: TcR
sequence annotation: L3S3P22 Terminator (synthetic)
sequence annotation: rrnC
sequence annotation: L3S2P11 Terminator
sequence annotation: pstS
sequence annotation: pelB signal sequence
sequence annotation: sfGFP
sequence annotation: Terminator rrnB T1
sequence annotation: rhaS
sequence annotation: glmS
sequence annotation: rhaR
sequence annotation: EM7 promoter
sequence: https://synbiohub.org/public/SEGA/SEGA091_seq/1

galK
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
EC number: 2.7.1.6
codon start: 1
db xref: ASAP:ABE-0002568
db xref: EcoGene:EG10363
db xref: GI:16128725
db xref: GeneID:945358
db xref: UniProtKB/Swiss-Prot:P0A6T3
function: enzyme; Degradation of small molecules: Carbon compounds
gene: galK
gene synonym: ECK0746; galA; JW0740
label: galK
locus tag: b0757
note: galK
product: galactokinase
protein id: NP_415278.1
translation: MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVLPCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLDAPIVAHENYQWANYVRGVVKHLQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQEAENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTLVGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTVEAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGGGFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA091/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA091/annotation2/range/1

https://synbiohub.org/public/SEGA/SEGA091/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA091/1
start: 605
end: 1753
orientation: reverseComplement

rhaB promoter
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
label: rhaB promoter
note: promoter of the E. coli rhaBAD operon, conferring tight induction with L-rhamnose and repression with D-glucose in the presence of RhaR and RhaS (Giacalone et al., 2006)
note: rhaB promoter
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA091/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA091/annotation7/range/1

https://synbiohub.org/public/SEGA/SEGA091/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA091/1
start: 3800
end: 3918
orientation: inline

medium TIR
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
note: TIR
note: medium TIR
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA091/1
role: SO:0000139
location: https://synbiohub.org/public/SEGA/SEGA091/annotation8/range/1

https://synbiohub.org/public/SEGA/SEGA091/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA091/1
start: 3930
end: 3956
orientation: inline

Promoter 3BCD2
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
label: Promoter 3BCD2
note: Promoter 3BCD2
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA091/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA091/annotation12/range/1

https://synbiohub.org/public/SEGA/SEGA091/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA091/1
start: 4787
end: 4815
orientation: inline

TcR
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
codon start: 1
gene: tet
label: TcR
note: TcR
note: confers resistance to tetracycline
product: tetracycline efflux protein
translation: MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHYGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA091/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA091/annotation13/range/1

https://synbiohub.org/public/SEGA/SEGA091/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA091/1
start: 4855
end: 6045
orientation: inline

L3S3P22 Terminator (synthetic)
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
label: L3S3P22 Terminator (synthetic)
note: L3S3P22 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA091/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA091/annotation11/range/1

https://synbiohub.org/public/SEGA/SEGA091/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA091/1
start: 4728
end: 4776
orientation: inline

rrnC
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
label: rrnC
note: rrnC
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA091/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA091/annotation14/range/1

https://synbiohub.org/public/SEGA/SEGA091/annotation14/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA091/1
start: 6056
end: 6096
orientation: inline

L3S2P11 Terminator
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
label: L3S2P11 Terminator
note: L3S2P11 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA091/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA091/annotation1/range/1

https://synbiohub.org/public/SEGA/SEGA091/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA091/1
start: 538
end: 594
orientation: inline

pstS
type: SequenceAnnotation
persistent identity: annotation15
display id: annotation15
version: 1
codon start: 1
label: pstS
note: pstS
translation: MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKWADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA091/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA091/annotation15/range/1

https://synbiohub.org/public/SEGA/SEGA091/annotation15/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA091/1
start: 6291
end: 6538
orientation: inline

pelB signal sequence
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
codon start: 1
gene: pelB (fragment)
label: pelB signal sequence
note: pelB signal sequence
product: leader peptide for secretion
translation: MKYLLPTAAAGLLLLAAQPAMA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA091/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA091/annotation9/range/1

https://synbiohub.org/public/SEGA/SEGA091/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA091/1
start: 3948
end: 4013
orientation: inline

sfGFP
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
codon start: 1
label: sfGFP
note: sfGFP
product: superfolder GFP
translation: RKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDGTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEFVTAAGITHGMDELYK
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA091/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA091/annotation10/range/1

https://synbiohub.org/public/SEGA/SEGA091/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA091/1
start: 4014
end: 4724
orientation: inline

Terminator rrnB T1
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
label: Terminator rrnB T1
note: Terminator rrnB T1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA091/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA091/annotation4/range/1

https://synbiohub.org/public/SEGA/SEGA091/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA091/1
start: 1843
end: 1886
orientation: inline

rhaS
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
codon start: 1
gene: E. coli rhaS
label: rhaS
note: activated by L-rhamnose
note: rhaS
product: positive regulator of the rhaB promoter
translation: MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHGTGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLLPQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKSSLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQRYLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGFLQ
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA091/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA091/annotation6/range/1

https://synbiohub.org/public/SEGA/SEGA091/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA091/1
start: 2818
end: 3654
orientation: reverseComplement

glmS
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
codon start: 1
label: glmS
note: glmS
translation: VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA091/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA091/annotation0/range/1

https://synbiohub.org/public/SEGA/SEGA091/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA091/1
start: 1
end: 528
orientation: inline

rhaR
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
codon start: 1
gene: E. coli rhaR
label: rhaR
note: needed for rhamnose-dependent activation of the rhaB promoter
note: rhaR
product: transcriptional activator of rhaR and rhaS
translation: MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQGAIPGFNASAGQPHWRLGSMGMAQARQVIGQLEHESSQHVPFANEMAELLFGQLVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRHLNSQKD
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA091/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA091/annotation5/range/1

https://synbiohub.org/public/SEGA/SEGA091/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA091/1
start: 1899
end: 2744
orientation: reverseComplement

EM7 promoter
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
label: EM7 promoter
note: EM7 promoter
note: synthetic bacterial promoter
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA091/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA091/annotation3/range/1

https://synbiohub.org/public/SEGA/SEGA091/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA091/1
start: 1783
end: 1830
orientation: reverseComplement

https://synbiohub.org/public/SEGA/SEGA033/1
type: ComponentDefinition
persistent identity: SEGA033
display id: SEGA033
version: 1
description: natural linear DNA.
owned by: carbay
owned by: morno
date: 08-SEP-2021
division: UNA
keywords: .
locus: SEGA033
molecule: ds-DNA
organism: unspecified
reference: https://synbiohub.org/user/carbay/SEGA/SEGA033_reference0/1
source: natural DNA sequence
type: DnaRegion
type: SO:0000987
role: SO:0000804
sequence annotation: rrnC
sequence annotation: medium TIR
sequence annotation: pelB signal sequence
sequence annotation: TcR
sequence annotation: sfGFP
sequence annotation: trc promoter
sequence annotation: L3S2P11 Terminator
sequence annotation: lacI
sequence annotation: pstS
sequence annotation: glmS
sequence annotation: L3S3P22 Terminator (synthetic)
sequence annotation: lacI promoter
sequence annotation: lacO
sequence annotation: Promoter 3BCD2
sequence: https://synbiohub.org/public/SEGA/SEGA033_seq/1

rrnC
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
label: rrnC
note: rrnC
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA033/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA033/annotation12/range/1

https://synbiohub.org/public/SEGA/SEGA033/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA033/1
start: 3986
end: 4026
orientation: inline

medium TIR
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
note: TIR
note: medium TIR
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA033/1
role: SO:0000139
location: https://synbiohub.org/public/SEGA/SEGA033/annotation6/range/1

https://synbiohub.org/public/SEGA/SEGA033/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA033/1
start: 1860
end: 1886
orientation: inline

pelB signal sequence
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
codon start: 1
gene: pelB (fragment)
label: pelB signal sequence
note: pelB signal sequence
product: leader peptide for secretion
translation: MKYLLPTAAAGLLLLAAQPAMA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA033/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA033/annotation7/range/1

https://synbiohub.org/public/SEGA/SEGA033/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA033/1
start: 1878
end: 1943
orientation: inline

TcR
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
codon start: 1
gene: tet
label: TcR
note: TcR
note: confers resistance to tetracycline
product: tetracycline efflux protein
translation: MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHYGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVIFGEDRFRWSATMSGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA033/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA033/annotation11/range/1

https://synbiohub.org/public/SEGA/SEGA033/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA033/1
start: 2785
end: 3975
orientation: inline

sfGFP
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
codon start: 1
label: sfGFP
note: sfGFP
product: superfolder GFP
translation: RKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDGTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEFVTAAGITHGMDELYK
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA033/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA033/annotation8/range/1

https://synbiohub.org/public/SEGA/SEGA033/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA033/1
start: 1944
end: 2654
orientation: inline

trc promoter
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
label: trc promoter
note: strong E. coli promoter; hybrid between the trp and lac UV5 promoters
note: trc promoter
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA033/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA033/annotation4/range/1

https://synbiohub.org/public/SEGA/SEGA033/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA033/1
start: 1795
end: 1824
orientation: inline

L3S2P11 Terminator
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
label: L3S2P11 Terminator
note: L3S2P11 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA033/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA033/annotation1/range/1

https://synbiohub.org/public/SEGA/SEGA033/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA033/1
start: 538
end: 594
orientation: inline

lacI
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
codon start: 1
gene: lacI
label: lacI
note: The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG).
note: lacI
product: lac repressor
translation: MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA033/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA033/annotation2/range/1

https://synbiohub.org/public/SEGA/SEGA033/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA033/1
start: 604
end: 1686
orientation: reverseComplement

pstS
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
codon start: 1
label: pstS
note: pstS
translation: MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKWADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA033/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA033/annotation13/range/1

https://synbiohub.org/public/SEGA/SEGA033/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA033/1
start: 4221
end: 4468
orientation: inline

glmS
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
codon start: 1
label: glmS
note: glmS
translation: VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA033/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA033/annotation0/range/1

https://synbiohub.org/public/SEGA/SEGA033/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA033/1
start: 1
end: 528
orientation: inline

L3S3P22 Terminator (synthetic)
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
label: L3S3P22 Terminator (synthetic)
note: L3S3P22 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA033/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA033/annotation9/range/1

https://synbiohub.org/public/SEGA/SEGA033/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA033/1
start: 2658
end: 2706
orientation: inline

lacI promoter
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
gene: lacI
label: lacI promoter
note: lacI promoter
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA033/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA033/annotation3/range/1

https://synbiohub.org/public/SEGA/SEGA033/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA033/1
start: 1687
end: 1764
orientation: reverseComplement

lacO
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
bound moiety: lac repressor
feature type: protein_bind
label: lacO
note: lac operator
note: lacO
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA033/1
role: SO:0000110
location: https://synbiohub.org/public/SEGA/SEGA033/annotation5/range/1

https://synbiohub.org/public/SEGA/SEGA033/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA033/1
start: 1825
end: 1848
orientation: inline

Promoter 3BCD2
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
label: Promoter 3BCD2
note: Promoter 3BCD2
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA033/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA033/annotation10/range/1

https://synbiohub.org/public/SEGA/SEGA033/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA033/1
start: 2717
end: 2745
orientation: inline

https://synbiohub.org/public/SEGA/SEGA019/1
type: ComponentDefinition
persistent identity: SEGA019
display id: SEGA019
version: 1
description: natural linear DNA.
owned by: carbay
owned by: morno
date: 09-SEP-2021
division: UNA
keywords: .
locus: SEGA019
molecule: ds-DNA
organism: unspecified
reference: https://synbiohub.org/public/SEGA/reference0_1/1
source: natural DNA sequence
type: DnaRegion
type: SO:0000987
role: SO:0000804
sequence annotation: glmS
sequence annotation: Promoter 3BCD2
sequence annotation: high TIR
sequence annotation: pstS
sequence annotation: rrnC
sequence annotation: P-J23100
sequence annotation: L3S3P22 Terminator (synthetic)
sequence annotation: L3S2P11 Terminator
sequence annotation: TcR
sequence annotation: sfGFP
sequence annotation: pelB signal sequence
sequence: https://synbiohub.org/public/SEGA/SEGA019_seq/1

glmS
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
codon start: 1
label: glmS
note: glmS
translation: VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA019/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA019/annotation0/range/1

https://synbiohub.org/public/SEGA/SEGA019/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA019/1
start: 1
end: 528
orientation: inline

Promoter 3BCD2
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
label: Promoter 3BCD2
note: Promoter 3BCD2
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA019/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA019/annotation7/range/1

https://synbiohub.org/public/SEGA/SEGA019/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA019/1
start: 1507
end: 1535
orientation: inline

high TIR
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
note: TIR
note: high TIR
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA019/1
role: SO:0000139
location: https://synbiohub.org/public/SEGA/SEGA019/annotation3/range/1

https://synbiohub.org/public/SEGA/SEGA019/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA019/1
start: 650
end: 676
orientation: inline

pstS
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
codon start: 1
label: pstS
note: pstS
translation: MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKWADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA019/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA019/annotation10/range/1

https://synbiohub.org/public/SEGA/SEGA019/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA019/1
start: 3011
end: 3258
orientation: inline

rrnC
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
label: rrnC
note: rrnC
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA019/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA019/annotation9/range/1

https://synbiohub.org/public/SEGA/SEGA019/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA019/1
start: 2776
end: 2816
orientation: inline

P-J23100
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
label: P-J23100
note: P-J23100
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA019/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA019/annotation2/range/1

https://synbiohub.org/public/SEGA/SEGA019/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA019/1
start: 604
end: 638
orientation: inline

L3S3P22 Terminator (synthetic)
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
label: L3S3P22 Terminator (synthetic)
note: L3S3P22 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA019/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA019/annotation6/range/1

https://synbiohub.org/public/SEGA/SEGA019/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA019/1
start: 1448
end: 1496
orientation: inline

L3S2P11 Terminator
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
label: L3S2P11 Terminator
note: L3S2P11 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA019/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA019/annotation1/range/1

https://synbiohub.org/public/SEGA/SEGA019/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA019/1
start: 538
end: 594
orientation: inline

TcR
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
codon start: 1
gene: tet
label: TcR
note: TcR
note: confers resistance to tetracycline
product: tetracycline efflux protein
translation: MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHYGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVIFGEDRFRWSATMSGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA019/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA019/annotation8/range/1

https://synbiohub.org/public/SEGA/SEGA019/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA019/1
start: 1575
end: 2765
orientation: inline

sfGFP
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
codon start: 1
label: sfGFP
note: sfGFP
product: superfolder GFP
translation: RKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDGTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEFVTAAGITHGMDELYK
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA019/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA019/annotation5/range/1

https://synbiohub.org/public/SEGA/SEGA019/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA019/1
start: 734
end: 1444
orientation: inline

pelB signal sequence
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
codon start: 1
gene: pelB (fragment)
label: pelB signal sequence
note: pelB signal sequence
product: leader peptide for secretion
translation: MKYLLPTAAAGLLLLAAQPAMA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA019/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA019/annotation4/range/1

https://synbiohub.org/public/SEGA/SEGA019/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA019/1
start: 668
end: 733
orientation: inline

https://synbiohub.org/public/SEGA/SEGA020/1
type: ComponentDefinition
persistent identity: SEGA020
display id: SEGA020
version: 1
description: natural linear DNA.
owned by: carbay
owned by: morno
date: 09-SEP-2021
division: UNA
keywords: .
locus: SEGA020
molecule: ds-DNA
organism: unspecified
reference: https://synbiohub.org/public/SEGA/reference0_1/1
source: natural DNA sequence
type: DnaRegion
type: SO:0000987
role: SO:0000804
sequence annotation: The trc promoter combines the -35 region from the trp promoter and the -10 region from the lacUV5 promoter
sequence annotation: L3S3P22 Terminator (synthetic)
sequence annotation: Promoter 3BCD2
sequence annotation: lacO
sequence annotation: lacI
sequence annotation: low TIR
sequence annotation: pstS
sequence annotation: glmS
sequence annotation: GFP
sequence annotation: lacI promoter
sequence annotation: rrnC
sequence annotation: L3S2P11 Terminator
sequence annotation: BCD
sequence annotation: TcR
sequence: https://synbiohub.org/public/SEGA/SEGA020_seq/1

The trc promoter combines the -35 region from the trp promoter and the -10 region from the lacUV5 promoter
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
note: Ptrc
note: The trc promoter combines the -35 region from the trp promoter and the -10 region from the lacUV5 promoter
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA020/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA020/annotation4/range/1

https://synbiohub.org/public/SEGA/SEGA020/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA020/1
start: 1795
end: 1824
orientation: inline

L3S3P22 Terminator (synthetic)
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
label: L3S3P22 Terminator (synthetic)
note: L3S3P22 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA020/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA020/annotation9/range/1

https://synbiohub.org/public/SEGA/SEGA020/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA020/1
start: 2648
end: 2696
orientation: inline

Promoter 3BCD2
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
label: Promoter 3BCD2
note: Promoter 3BCD2
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA020/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA020/annotation10/range/1

https://synbiohub.org/public/SEGA/SEGA020/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA020/1
start: 2707
end: 2735
orientation: inline

lacO
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
allele: lac operator
bound moiety: lac repressor
feature type: protein_bind
label: lacO
note: lac operator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA020/1
role: SO:0000110
location: https://synbiohub.org/public/SEGA/SEGA020/annotation5/range/1

https://synbiohub.org/public/SEGA/SEGA020/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA020/1
start: 1825
end: 1848
orientation: inline

lacI
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
codon start: 1
gene: lacI
label: lacI
note: The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG).
note: lacI
product: lac repressor
translation: MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA020/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA020/annotation2/range/1

https://synbiohub.org/public/SEGA/SEGA020/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA020/1
start: 604
end: 1686
orientation: reverseComplement

low TIR
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
note: TIR
note: low TIR
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA020/1
role: SO:0000139
location: https://synbiohub.org/public/SEGA/SEGA020/annotation6/range/1

https://synbiohub.org/public/SEGA/SEGA020/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA020/1
start: 1860
end: 1886
orientation: inline

pstS
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
codon start: 1
label: pstS
note: pstS
translation: MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKWADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA020/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA020/annotation13/range/1

https://synbiohub.org/public/SEGA/SEGA020/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA020/1
start: 4211
end: 4458
orientation: inline

glmS
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
codon start: 1
label: glmS
note: glmS
translation: VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA020/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA020/annotation0/range/1

https://synbiohub.org/public/SEGA/SEGA020/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA020/1
start: 1
end: 528
orientation: inline

GFP
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
codon start: 1
label: GFP
note: GFP
product: green fluorescent protein
translation: SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYK
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA020/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA020/annotation8/range/1

https://synbiohub.org/public/SEGA/SEGA020/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA020/1
start: 1934
end: 2644
orientation: inline

lacI promoter
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
gene: lacI
label: lacI promoter
note: lacI promoter
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA020/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA020/annotation3/range/1

https://synbiohub.org/public/SEGA/SEGA020/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA020/1
start: 1687
end: 1764
orientation: reverseComplement

rrnC
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
label: rrnC
note: rrnC
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA020/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA020/annotation12/range/1

https://synbiohub.org/public/SEGA/SEGA020/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA020/1
start: 3976
end: 4016
orientation: inline

L3S2P11 Terminator
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
label: L3S2P11 Terminator
note: L3S2P11 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA020/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA020/annotation1/range/1

https://synbiohub.org/public/SEGA/SEGA020/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA020/1
start: 538
end: 594
orientation: inline

BCD
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
codon start: 1
label: BCD
note: BCD
translation: MKAIFVLKHLNHAKEVF
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA020/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA020/annotation7/range/1

https://synbiohub.org/public/SEGA/SEGA020/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA020/1
start: 1878
end: 1933
orientation: inline

TcR
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
codon start: 1
gene: tet
label: TcR
note: TcR
note: confers resistance to tetracycline
product: tetracycline efflux protein
translation: MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHYGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVIFGEDRFRWSATMSGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA020/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA020/annotation11/range/1

https://synbiohub.org/public/SEGA/SEGA020/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA020/1
start: 2775
end: 3965
orientation: inline

https://synbiohub.org/public/SEGA/SEGA095/1
type: ComponentDefinition
persistent identity: SEGA095
display id: SEGA095
version: 1
description: natural linear DNA.
owned by: carbay
owned by: morno
date: 09-SEP-2021
division: UNA
keywords: .
locus: SEGA095
molecule: ds-DNA
organism: unspecified
reference: https://synbiohub.org/public/SEGA/reference0_1/1
source: natural DNA sequence
type: DnaRegion
type: SO:0000987
role: SO:0000804
sequence annotation: L3S2P11 Terminator
sequence annotation: Terminator rrnB T1
sequence annotation: pstS
sequence annotation: Promoter 3BCD2
sequence annotation: EM7 promoter
sequence annotation: TcR
sequence annotation: rrnC
sequence annotation: sfGFP
sequence annotation: glmS
sequence annotation: YebFss
sequence annotation: galK
sequence annotation: high TIR
sequence annotation: YebF
sequence annotation: rhaS
sequence annotation: L3S3P22 Terminator (synthetic)
sequence annotation: rhaR
sequence annotation: rhaB promoter
sequence: https://synbiohub.org/public/SEGA/SEGA095_seq/1

L3S2P11 Terminator
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
label: L3S2P11 Terminator
note: L3S2P11 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA095/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA095/annotation1/range/1

https://synbiohub.org/public/SEGA/SEGA095/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA095/1
start: 538
end: 594
orientation: inline

Terminator rrnB T1
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
label: Terminator rrnB T1
note: Terminator rrnB T1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA095/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA095/annotation4/range/1

https://synbiohub.org/public/SEGA/SEGA095/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA095/1
start: 1843
end: 1886
orientation: inline

pstS
type: SequenceAnnotation
persistent identity: annotation16
display id: annotation16
version: 1
codon start: 1
label: pstS
note: pstS
translation: MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKWADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA095/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA095/annotation16/range/1

https://synbiohub.org/public/SEGA/SEGA095/annotation16/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA095/1
start: 6579
end: 6826
orientation: inline

Promoter 3BCD2
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
label: Promoter 3BCD2
note: Promoter 3BCD2
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA095/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA095/annotation13/range/1

https://synbiohub.org/public/SEGA/SEGA095/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA095/1
start: 5075
end: 5103
orientation: inline

EM7 promoter
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
label: EM7 promoter
note: EM7 promoter
note: synthetic bacterial promoter
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA095/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA095/annotation3/range/1

https://synbiohub.org/public/SEGA/SEGA095/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA095/1
start: 1783
end: 1830
orientation: reverseComplement

TcR
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
codon start: 1
gene: tet
label: TcR
note: TcR
note: confers resistance to tetracycline
product: tetracycline efflux protein
translation: MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHYGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA095/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA095/annotation14/range/1

https://synbiohub.org/public/SEGA/SEGA095/annotation14/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA095/1
start: 5143
end: 6333
orientation: inline

rrnC
type: SequenceAnnotation
persistent identity: annotation15
display id: annotation15
version: 1
label: rrnC
note: rrnC
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA095/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA095/annotation15/range/1

https://synbiohub.org/public/SEGA/SEGA095/annotation15/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA095/1
start: 6344
end: 6384
orientation: inline

sfGFP
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
codon start: 1
label: sfGFP
note: sfGFP
product: superfolder GFP
translation: RKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDGTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEFVTAAGITHGMDELYK
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA095/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA095/annotation11/range/1

https://synbiohub.org/public/SEGA/SEGA095/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA095/1
start: 4302
end: 5012
orientation: inline

glmS
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
codon start: 1
label: glmS
note: glmS
translation: VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA095/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA095/annotation0/range/1

https://synbiohub.org/public/SEGA/SEGA095/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA095/1
start: 1
end: 528
orientation: inline

YebFss
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
codon start: 1
db xref: ASAP:ABE-0006141
db xref: EcoGene:EG11807
db xref: GI:145698278
db xref: GeneID:946363
db xref: UniProtKB/Swiss-Prot:P33219
gene: yebF
gene synonym: ECK1848; JW1836
label: YebFss
locus tag: b1847
note: YebFss
product: yebF signal sequence
protein id: NP_416361.2
translation: MKKRGAFLGLLLVSACASVFA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA095/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA095/annotation9/range/1

https://synbiohub.org/public/SEGA/SEGA095/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA095/1
start: 3948
end: 4010
orientation: inline

galK
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
EC number: 2.7.1.6
codon start: 1
db xref: ASAP:ABE-0002568
db xref: EcoGene:EG10363
db xref: GI:16128725
db xref: GeneID:945358
db xref: UniProtKB/Swiss-Prot:P0A6T3
function: enzyme; Degradation of small molecules: Carbon compounds
gene: galK
gene synonym: ECK0746; galA; JW0740
label: galK
locus tag: b0757
note: galK
product: galactokinase
protein id: NP_415278.1
translation: MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVLPCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLDAPIVAHENYQWANYVRGVVKHLQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQEAENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTLVGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTVEAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGGGFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA095/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA095/annotation2/range/1

https://synbiohub.org/public/SEGA/SEGA095/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA095/1
start: 605
end: 1753
orientation: reverseComplement

high TIR
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
note: TIR
note: high TIR
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA095/1
role: SO:0000139
location: https://synbiohub.org/public/SEGA/SEGA095/annotation8/range/1

https://synbiohub.org/public/SEGA/SEGA095/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA095/1
start: 3930
end: 3956
orientation: inline

YebF
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
codon start: 1
label: YebF
note: YebF
product: full size yebF protein
translation: ANNETSKSVTFPKCEDLDAAGIAASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHYQVSVDCKAGMAEYQRR
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA095/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA095/annotation10/range/1

https://synbiohub.org/public/SEGA/SEGA095/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA095/1
start: 4011
end: 4301
orientation: inline

rhaS
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
codon start: 1
gene: E. coli rhaS
label: rhaS
note: activated by L-rhamnose
note: rhaS
product: positive regulator of the rhaB promoter
translation: MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHGTGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLLPQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKSSLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQRYLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGFLQ
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA095/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA095/annotation6/range/1

https://synbiohub.org/public/SEGA/SEGA095/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA095/1
start: 2818
end: 3654
orientation: reverseComplement

L3S3P22 Terminator (synthetic)
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
label: L3S3P22 Terminator (synthetic)
note: L3S3P22 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA095/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA095/annotation12/range/1

https://synbiohub.org/public/SEGA/SEGA095/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA095/1
start: 5016
end: 5064
orientation: inline

rhaR
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
codon start: 1
gene: E. coli rhaR
label: rhaR
note: needed for rhamnose-dependent activation of the rhaB promoter
note: rhaR
product: transcriptional activator of rhaR and rhaS
translation: MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQGAIPGFNASAGQPHWRLGSMGMAQARQVIGQLEHESSQHVPFANEMAELLFGQLVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRHLNSQKD
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA095/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA095/annotation5/range/1

https://synbiohub.org/public/SEGA/SEGA095/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA095/1
start: 1899
end: 2744
orientation: reverseComplement

rhaB promoter
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
label: rhaB promoter
note: promoter of the E. coli rhaBAD operon, conferring tight induction with L-rhamnose and repression with D-glucose in the presence of RhaR and RhaS (Giacalone et al., 2006)
note: rhaB promoter
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA095/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA095/annotation7/range/1

https://synbiohub.org/public/SEGA/SEGA095/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA095/1
start: 3800
end: 3918
orientation: inline

https://synbiohub.org/public/SEGA/SEGA098/1
type: ComponentDefinition
persistent identity: SEGA098
display id: SEGA098
version: 1
description: natural linear DNA.
owned by: carbay
owned by: morno
date: 09-SEP-2021
division: UNA
keywords: .
locus: SEGA098
molecule: ds-DNA
organism: unspecified
reference: https://synbiohub.org/public/SEGA/reference0_1/1
source: natural DNA sequence
type: DnaRegion
type: SO:0000987
role: SO:0000804
sequence annotation: P-J23100
sequence annotation: high TIR
sequence annotation: rrnC
sequence annotation: GFP
sequence annotation: BCD
sequence annotation: pstS
sequence annotation: TcR
sequence annotation: L3S2P11 Terminator
sequence annotation: glmS
sequence: https://synbiohub.org/public/SEGA/SEGA098_seq/1

P-J23100
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
label: P-J23100
note: P-J23100
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA098/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA098/annotation2/range/1

https://synbiohub.org/public/SEGA/SEGA098/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA098/1
start: 604
end: 638
orientation: inline

high TIR
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
note: TIR
note: high TIR
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA098/1
role: SO:0000139
location: https://synbiohub.org/public/SEGA/SEGA098/annotation3/range/1

https://synbiohub.org/public/SEGA/SEGA098/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA098/1
start: 650
end: 676
orientation: inline

rrnC
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
label: rrnC
note: rrnC
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA098/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA098/annotation7/range/1

https://synbiohub.org/public/SEGA/SEGA098/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA098/1
start: 2564
end: 2604
orientation: inline

GFP
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
codon start: 1
label: GFP
note: GFP
product: green fluorescent protein
translation: SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYK
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA098/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA098/annotation5/range/1

https://synbiohub.org/public/SEGA/SEGA098/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA098/1
start: 724
end: 1434
orientation: inline

BCD
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
codon start: 1
label: BCD
note: BCD
translation: MKAIFVLKHLNHAKEVF
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA098/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA098/annotation4/range/1

https://synbiohub.org/public/SEGA/SEGA098/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA098/1
start: 668
end: 723
orientation: inline

pstS
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
codon start: 1
label: pstS
note: pstS
translation: MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKWADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA098/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA098/annotation8/range/1

https://synbiohub.org/public/SEGA/SEGA098/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA098/1
start: 2799
end: 3046
orientation: inline

TcR
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
codon start: 1
gene: tet
label: TcR
note: TcR
note: confers resistance to tetracycline
product: tetracycline efflux protein
translation: VLPGLLRDIVHSDSIASHYGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVIFGEDRFRWSATMSGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA098/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA098/annotation6/range/1

https://synbiohub.org/public/SEGA/SEGA098/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA098/1
start: 1441
end: 2553
orientation: inline

L3S2P11 Terminator
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
label: L3S2P11 Terminator
note: L3S2P11 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA098/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA098/annotation1/range/1

https://synbiohub.org/public/SEGA/SEGA098/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA098/1
start: 538
end: 594
orientation: inline

glmS
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
codon start: 1
label: glmS
note: glmS
translation: VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA098/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA098/annotation0/range/1

https://synbiohub.org/public/SEGA/SEGA098/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA098/1
start: 1
end: 528
orientation: inline

https://synbiohub.org/public/SEGA/SEGA093/1
type: ComponentDefinition
persistent identity: SEGA093
display id: SEGA093
version: 1
description: natural linear DNA.
owned by: carbay
owned by: morno
date: 09-SEP-2021
division: UNA
keywords: .
locus: SEGA093
molecule: ds-DNA
organism: unspecified
reference: https://synbiohub.org/user/carbay/SEGA/SEGA093_reference0/1
source: natural DNA sequence
type: DnaRegion
type: SO:0000987
role: SO:0000804
sequence annotation: glmS
sequence annotation: YebFss
sequence annotation: pstS
sequence annotation: Terminator rrnB T1
sequence annotation: YebF
sequence annotation: rrnC
sequence annotation: EM7 promoter
sequence annotation: rhaS
sequence annotation: rhaB promoter
sequence annotation: rhaR
sequence annotation: L3S3P22 Terminator (synthetic)
sequence annotation: sfGFP
sequence annotation: low TIR
sequence annotation: L3S2P11 Terminator
sequence annotation: TcR
sequence annotation: Promoter 3BCD2
sequence annotation: galK
sequence: https://synbiohub.org/public/SEGA/SEGA093_seq/1

glmS
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
codon start: 1
label: glmS
note: glmS
translation: VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA093/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA093/annotation0/range/1

https://synbiohub.org/public/SEGA/SEGA093/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA093/1
start: 1
end: 528
orientation: inline

YebFss
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
codon start: 1
db xref: ASAP:ABE-0006141
db xref: EcoGene:EG11807
db xref: GI:145698278
db xref: GeneID:946363
db xref: UniProtKB/Swiss-Prot:P33219
gene: yebF
gene synonym: ECK1848; JW1836
label: YebFss
locus tag: b1847
note: YebFss
product: yebF signal sequence
protein id: NP_416361.2
translation: MKKRGAFLGLLLVSACASVFA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA093/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA093/annotation9/range/1

https://synbiohub.org/public/SEGA/SEGA093/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA093/1
start: 3948
end: 4010
orientation: inline

pstS
type: SequenceAnnotation
persistent identity: annotation16
display id: annotation16
version: 1
codon start: 1
label: pstS
note: pstS
translation: MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKWADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA093/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA093/annotation16/range/1

https://synbiohub.org/public/SEGA/SEGA093/annotation16/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA093/1
start: 6579
end: 6826
orientation: inline

Terminator rrnB T1
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
label: Terminator rrnB T1
note: Terminator rrnB T1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA093/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA093/annotation4/range/1

https://synbiohub.org/public/SEGA/SEGA093/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA093/1
start: 1843
end: 1886
orientation: inline

YebF
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
codon start: 1
label: YebF
note: YebF
product: full size yebF protein
translation: ANNETSKSVTFPKCEDLDAAGIAASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHYQVSVDCKAGMAEYQRR
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA093/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA093/annotation10/range/1

https://synbiohub.org/public/SEGA/SEGA093/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA093/1
start: 4011
end: 4301
orientation: inline

rrnC
type: SequenceAnnotation
persistent identity: annotation15
display id: annotation15
version: 1
label: rrnC
note: rrnC
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA093/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA093/annotation15/range/1

https://synbiohub.org/public/SEGA/SEGA093/annotation15/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA093/1
start: 6344
end: 6384
orientation: inline

EM7 promoter
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
label: EM7 promoter
note: EM7 promoter
note: synthetic bacterial promoter
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA093/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA093/annotation3/range/1

https://synbiohub.org/public/SEGA/SEGA093/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA093/1
start: 1783
end: 1830
orientation: reverseComplement

rhaS
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
codon start: 1
gene: E. coli rhaS
label: rhaS
note: activated by L-rhamnose
note: rhaS
product: positive regulator of the rhaB promoter
translation: MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHGTGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLLPQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKSSLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQRYLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGFLQ
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA093/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA093/annotation6/range/1

https://synbiohub.org/public/SEGA/SEGA093/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA093/1
start: 2818
end: 3654
orientation: reverseComplement

rhaB promoter
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
label: rhaB promoter
note: promoter of the E. coli rhaBAD operon, conferring tight induction with L-rhamnose and repression with D-glucose in the presence of RhaR and RhaS (Giacalone et al., 2006)
note: rhaB promoter
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA093/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA093/annotation7/range/1

https://synbiohub.org/public/SEGA/SEGA093/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA093/1
start: 3800
end: 3918
orientation: inline

rhaR
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
codon start: 1
gene: E. coli rhaR
label: rhaR
note: needed for rhamnose-dependent activation of the rhaB promoter
note: rhaR
product: transcriptional activator of rhaR and rhaS
translation: MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQGAIPGFNASAGQPHWRLGSMGMAQARQVIGQLEHESSQHVPFANEMAELLFGQLVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRHLNSQKD
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA093/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA093/annotation5/range/1

https://synbiohub.org/public/SEGA/SEGA093/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA093/1
start: 1899
end: 2744
orientation: reverseComplement

L3S3P22 Terminator (synthetic)
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
label: L3S3P22 Terminator (synthetic)
note: L3S3P22 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA093/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA093/annotation12/range/1

https://synbiohub.org/public/SEGA/SEGA093/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA093/1
start: 5016
end: 5064
orientation: inline

sfGFP
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
codon start: 1
label: sfGFP
note: sfGFP
product: superfolder GFP
translation: RKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDGTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEFVTAAGITHGMDELYK
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA093/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA093/annotation11/range/1

https://synbiohub.org/public/SEGA/SEGA093/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA093/1
start: 4302
end: 5012
orientation: inline

low TIR
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
note: TIR
note: low TIR
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA093/1
role: SO:0000139
location: https://synbiohub.org/public/SEGA/SEGA093/annotation8/range/1

https://synbiohub.org/public/SEGA/SEGA093/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA093/1
start: 3930
end: 3956
orientation: inline

L3S2P11 Terminator
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
label: L3S2P11 Terminator
note: L3S2P11 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA093/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA093/annotation1/range/1

https://synbiohub.org/public/SEGA/SEGA093/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA093/1
start: 538
end: 594
orientation: inline

TcR
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
codon start: 1
gene: tet
label: TcR
note: TcR
note: confers resistance to tetracycline
product: tetracycline efflux protein
translation: MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHYGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA093/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA093/annotation14/range/1

https://synbiohub.org/public/SEGA/SEGA093/annotation14/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA093/1
start: 5143
end: 6333
orientation: inline

Promoter 3BCD2
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
label: Promoter 3BCD2
note: Promoter 3BCD2
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA093/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA093/annotation13/range/1

https://synbiohub.org/public/SEGA/SEGA093/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA093/1
start: 5075
end: 5103
orientation: inline

galK
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
EC number: 2.7.1.6
codon start: 1
db xref: ASAP:ABE-0002568
db xref: EcoGene:EG10363
db xref: GI:16128725
db xref: GeneID:945358
db xref: UniProtKB/Swiss-Prot:P0A6T3
function: enzyme; Degradation of small molecules: Carbon compounds
gene: galK
gene synonym: ECK0746; galA; JW0740
label: galK
locus tag: b0757
note: galK
product: galactokinase
protein id: NP_415278.1
translation: MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVLPCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLDAPIVAHENYQWANYVRGVVKHLQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQEAENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTLVGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTVEAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGGGFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA093/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA093/annotation2/range/1

https://synbiohub.org/public/SEGA/SEGA093/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA093/1
start: 605
end: 1753
orientation: reverseComplement

https://synbiohub.org/public/SEGA/SEGA072/1
type: ComponentDefinition
persistent identity: SEGA072
display id: SEGA072
version: 1
description: natural linear DNA.
owned by: carbay
owned by: morno
date: 08-SEP-2021
division: UNA
keywords: .
locus: SEGA072
molecule: ds-DNA
organism: unspecified
reference: https://synbiohub.org/user/carbay/SEGA/SEGA072_reference0/1
source: natural DNA sequence
type: DnaRegion
type: SO:0000987
role: SO:0000804
sequence annotation: lacI
sequence annotation: glmS
sequence annotation: lac operator
sequence annotation: yebF
sequence annotation: lacI promoter
sequence annotation: L3S2P11 Terminator
sequence annotation: YebF
sequence annotation: rrnC
sequence annotation: L3S3P22 Terminator (synthetic)
sequence annotation: Promoter 3BCD2
sequence annotation: sfGFP
sequence annotation: medium TIR
sequence annotation: T7 promoter
sequence annotation: pstS
sequence annotation: TcR
sequence: https://synbiohub.org/public/SEGA/SEGA072_seq/1

lacI
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
codon start: 1
gene: lacI
label: lacI
note: The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG).
note: lacI
product: lac repressor
translation: MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA072/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA072/annotation2/range/1

https://synbiohub.org/public/SEGA/SEGA072/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA072/1
start: 604
end: 1686
orientation: reverseComplement

glmS
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
codon start: 1
label: glmS
note: glmS
translation: VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA072/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA072/annotation0/range/1

https://synbiohub.org/public/SEGA/SEGA072/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA072/1
start: 1
end: 528
orientation: inline

lac operator
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
bound moiety: lac repressor encoded by lacI
feature type: protein_bind
label: lac operator
note: The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG).
note: lac operator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA072/1
role: SO:0000110
location: https://synbiohub.org/public/SEGA/SEGA072/annotation5/range/1

https://synbiohub.org/public/SEGA/SEGA072/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA072/1
start: 1814
end: 1838
orientation: inline

yebF
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
codon start: 1
db xref: ASAP:ABE-0006141
db xref: EcoGene:EG11807
db xref: GI:145698278
db xref: GeneID:946363
db xref: UniProtKB/Swiss-Prot:P33219
gene: yebF
gene synonym: ECK1848; JW1836
label: yebF
locus tag: b1847
note: yebFss
product: yebF signal sequence
protein id: NP_416361.2
translation: MKKRGAFLGLLLVSACASVFA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA072/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA072/annotation7/range/1

https://synbiohub.org/public/SEGA/SEGA072/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA072/1
start: 1868
end: 1930
orientation: inline

lacI promoter
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
gene: lacI
label: lacI promoter
note: lacI promoter
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA072/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA072/annotation3/range/1

https://synbiohub.org/public/SEGA/SEGA072/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA072/1
start: 1687
end: 1764
orientation: reverseComplement

L3S2P11 Terminator
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
label: L3S2P11 Terminator
note: L3S2P11 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA072/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA072/annotation1/range/1

https://synbiohub.org/public/SEGA/SEGA072/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA072/1
start: 538
end: 594
orientation: inline

YebF
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
codon start: 1
label: YebF
note: YebF
product: full size yebF protein
translation: ANNETSKSVTFPKCEDLDAAGIAASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHYQVSVDCKAGMAEYQRR
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA072/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA072/annotation8/range/1

https://synbiohub.org/public/SEGA/SEGA072/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA072/1
start: 1931
end: 2221
orientation: inline

rrnC
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
label: rrnC
note: rrnC
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA072/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA072/annotation13/range/1

https://synbiohub.org/public/SEGA/SEGA072/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA072/1
start: 4264
end: 4304
orientation: inline

L3S3P22 Terminator (synthetic)
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
label: L3S3P22 Terminator (synthetic)
note: L3S3P22 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA072/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA072/annotation10/range/1

https://synbiohub.org/public/SEGA/SEGA072/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA072/1
start: 2936
end: 2984
orientation: inline

Promoter 3BCD2
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
label: Promoter 3BCD2
note: Promoter 3BCD2
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA072/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA072/annotation11/range/1

https://synbiohub.org/public/SEGA/SEGA072/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA072/1
start: 2995
end: 3023
orientation: inline

sfGFP
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
codon start: 1
label: sfGFP
note: sfGFP
product: superfolder GFP
translation: RKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDGTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEFVTAAGITHGMDELYK
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA072/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA072/annotation9/range/1

https://synbiohub.org/public/SEGA/SEGA072/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA072/1
start: 2222
end: 2932
orientation: inline

medium TIR
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
note: TIR
note: medium TIR
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA072/1
role: SO:0000139
location: https://synbiohub.org/public/SEGA/SEGA072/annotation6/range/1

https://synbiohub.org/public/SEGA/SEGA072/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA072/1
start: 1850
end: 1876
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
label: T7 promoter
note: T7 promoter
note: promoter for bacteriophage T7 RNA polymerase
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA072/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA072/annotation4/range/1

https://synbiohub.org/public/SEGA/SEGA072/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA072/1
start: 1795
end: 1813
orientation: inline

pstS
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
codon start: 1
label: pstS
note: pstS
translation: MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKWADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA072/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA072/annotation14/range/1

https://synbiohub.org/public/SEGA/SEGA072/annotation14/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA072/1
start: 4499
end: 4746
orientation: inline

TcR
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
codon start: 1
gene: tet
label: TcR
note: TcR
note: confers resistance to tetracycline
product: tetracycline efflux protein
translation: MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHYGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVIFGEDRFRWSATMSGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA072/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA072/annotation12/range/1

https://synbiohub.org/public/SEGA/SEGA072/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA072/1
start: 3063
end: 4253
orientation: inline

https://synbiohub.org/public/SEGA/SEGA041/1
type: ComponentDefinition
persistent identity: SEGA041
display id: SEGA041
version: 1
description: natural linear DNA.
owned by: carbay
owned by: morno
date: 08-SEP-2021
division: UNA
keywords: .
locus: SEGA041
molecule: ds-DNA
organism: unspecified
reference: https://synbiohub.org/public/SEGA/reference0_1/1
source: natural DNA sequence
type: DnaRegion
type: SO:0000987
role: SO:0000804
sequence annotation: GFP
sequence annotation: pstS
sequence annotation: rrnC
sequence annotation: low TIR
sequence annotation: Ubiquitin
sequence annotation: glmS
sequence annotation: rhaS
sequence annotation: Promoter 3BCD2
sequence annotation: L3S3P22 Terminator (synthetic)
sequence annotation: rhaB promoter
sequence annotation: rhaR
sequence annotation: TcR
sequence annotation: L3S2P11 Terminator
sequence: https://synbiohub.org/public/SEGA/SEGA041_seq/1

GFP
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
codon start: 1
label: GFP
note: GFP
product: green fluorescent protein
translation: SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYK
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA041/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA041/annotation7/range/1

https://synbiohub.org/public/SEGA/SEGA041/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA041/1
start: 2965
end: 3675
orientation: inline

pstS
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
codon start: 1
label: pstS
note: pstS
translation: MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKWADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA041/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA041/annotation12/range/1

https://synbiohub.org/public/SEGA/SEGA041/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA041/1
start: 5242
end: 5489
orientation: inline

rrnC
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
label: rrnC
note: rrnC
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA041/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA041/annotation11/range/1

https://synbiohub.org/public/SEGA/SEGA041/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA041/1
start: 5007
end: 5047
orientation: inline

low TIR
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
note: TIR
note: low TIR
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA041/1
role: SO:0000139
location: https://synbiohub.org/public/SEGA/SEGA041/annotation5/range/1

https://synbiohub.org/public/SEGA/SEGA041/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA041/1
start: 2638
end: 2664
orientation: inline

Ubiquitin
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
codon start: 1
label: Ubiquitin
note: Ubiquitin
translation: MQIFVKTLTGKTITLEVEPGSAHHHHHHHHHHAGSSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQEKSTLELVLELQNETQSATSDA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA041/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA041/annotation6/range/1

https://synbiohub.org/public/SEGA/SEGA041/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA041/1
start: 2656
end: 2955
orientation: inline

glmS
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
codon start: 1
label: glmS
note: glmS
translation: VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA041/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA041/annotation0/range/1

https://synbiohub.org/public/SEGA/SEGA041/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA041/1
start: 1
end: 528
orientation: inline

rhaS
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
codon start: 1
gene: E. coli rhaS
label: rhaS
note: activated by L-rhamnose
note: rhaS
product: positive regulator of the rhaB promoter
translation: MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHGTGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLLPQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKSSLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQRYLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGFLQ
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA041/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA041/annotation3/range/1

https://synbiohub.org/public/SEGA/SEGA041/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA041/1
start: 1526
end: 2362
orientation: reverseComplement

Promoter 3BCD2
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
label: Promoter 3BCD2
note: Promoter 3BCD2
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA041/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA041/annotation9/range/1

https://synbiohub.org/public/SEGA/SEGA041/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA041/1
start: 3738
end: 3766
orientation: inline

L3S3P22 Terminator (synthetic)
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
label: L3S3P22 Terminator (synthetic)
note: L3S3P22 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA041/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA041/annotation8/range/1

https://synbiohub.org/public/SEGA/SEGA041/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA041/1
start: 3679
end: 3727
orientation: inline

rhaB promoter
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
label: rhaB promoter
note: promoter of the E. coli rhaBAD operon, conferring tight induction with L-rhamnose and repression with D-glucose in the presence of RhaR and RhaS (Giacalone et al., 2006)
note: rhaB promoter
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA041/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA041/annotation4/range/1

https://synbiohub.org/public/SEGA/SEGA041/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA041/1
start: 2508
end: 2626
orientation: inline

rhaR
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
codon start: 1
gene: E. coli rhaR
label: rhaR
note: needed for rhamnose-dependent activation of the rhaB promoter
note: rhaR
product: transcriptional activator of rhaR and rhaS
translation: MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQGAIPGFNASAGQPHWRLGSMGMAQARQVIGQLEHESSQHVPFANEMAELLFGQLVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRHLNSQKD
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA041/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA041/annotation2/range/1

https://synbiohub.org/public/SEGA/SEGA041/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA041/1
start: 607
end: 1452
orientation: reverseComplement

TcR
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
codon start: 1
gene: tet
label: TcR
note: TcR
note: confers resistance to tetracycline
product: tetracycline efflux protein
translation: MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHYGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVIFGEDRFRWSATMSGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA041/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA041/annotation10/range/1

https://synbiohub.org/public/SEGA/SEGA041/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA041/1
start: 3806
end: 4996
orientation: inline

L3S2P11 Terminator
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
label: L3S2P11 Terminator
note: L3S2P11 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA041/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA041/annotation1/range/1

https://synbiohub.org/public/SEGA/SEGA041/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA041/1
start: 538
end: 594
orientation: inline

https://synbiohub.org/public/SEGA/SEGA023/1
type: ComponentDefinition
persistent identity: SEGA023
display id: SEGA023
version: 1
description: natural linear DNA.
owned by: carbay
owned by: morno
date: 08-SEP-2021
division: UNA
keywords: .
locus: SEGA023
molecule: ds-DNA
organism: unspecified
reference: https://synbiohub.org/public/SEGA/reference0_1/1
source: natural DNA sequence
type: DnaRegion
type: SO:0000987
role: SO:0000804
sequence annotation: pstS
sequence annotation: lacI promoter
sequence annotation: lacI
sequence annotation: glmS
sequence annotation: trc promoter
sequence annotation: Promoter 3BCD2
sequence annotation: GFP
sequence annotation: L3S2P11 Terminator
sequence annotation: low TIR
sequence annotation: rrnC
sequence annotation: TcR
sequence annotation: Ubiquitin
sequence annotation: lacO
sequence annotation: L3S3P22 Terminator (synthetic)
sequence: https://synbiohub.org/public/SEGA/SEGA023_seq/1

pstS
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
codon start: 1
label: pstS
note: pstS
translation: MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKWADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA023/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA023/annotation13/range/1

https://synbiohub.org/public/SEGA/SEGA023/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA023/1
start: 4464
end: 4711
orientation: inline

lacI promoter
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
gene: lacI
label: lacI promoter
note: lacI promoter
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA023/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA023/annotation3/range/1

https://synbiohub.org/public/SEGA/SEGA023/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA023/1
start: 1687
end: 1764
orientation: reverseComplement

lacI
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
codon start: 1
gene: lacI
label: lacI
note: The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG).
note: lacI
product: lac repressor
translation: MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA023/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA023/annotation2/range/1

https://synbiohub.org/public/SEGA/SEGA023/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA023/1
start: 604
end: 1686
orientation: reverseComplement

glmS
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
codon start: 1
label: glmS
note: glmS
translation: VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA023/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA023/annotation0/range/1

https://synbiohub.org/public/SEGA/SEGA023/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA023/1
start: 1
end: 528
orientation: inline

trc promoter
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
label: trc promoter
note: strong E. coli promoter; hybrid between the trp and lac UV5 promoters
note: trc promoter
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA023/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA023/annotation4/range/1

https://synbiohub.org/public/SEGA/SEGA023/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA023/1
start: 1795
end: 1824
orientation: inline

Promoter 3BCD2
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
label: Promoter 3BCD2
note: Promoter 3BCD2
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA023/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA023/annotation10/range/1

https://synbiohub.org/public/SEGA/SEGA023/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA023/1
start: 2960
end: 2988
orientation: inline

GFP
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
codon start: 1
label: GFP
note: GFP
product: green fluorescent protein
translation: SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYK
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA023/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA023/annotation8/range/1

https://synbiohub.org/public/SEGA/SEGA023/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA023/1
start: 2187
end: 2897
orientation: inline

L3S2P11 Terminator
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
label: L3S2P11 Terminator
note: L3S2P11 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA023/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA023/annotation1/range/1

https://synbiohub.org/public/SEGA/SEGA023/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA023/1
start: 538
end: 594
orientation: inline

low TIR
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
note: TIR
note: low TIR
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA023/1
role: SO:0000139
location: https://synbiohub.org/public/SEGA/SEGA023/annotation6/range/1

https://synbiohub.org/public/SEGA/SEGA023/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA023/1
start: 1860
end: 1886
orientation: inline

rrnC
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
label: rrnC
note: rrnC
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA023/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA023/annotation12/range/1

https://synbiohub.org/public/SEGA/SEGA023/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA023/1
start: 4229
end: 4269
orientation: inline

TcR
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
codon start: 1
gene: tet
label: TcR
note: TcR
note: confers resistance to tetracycline
product: tetracycline efflux protein
translation: MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHYGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVIFGEDRFRWSATMSGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA023/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA023/annotation11/range/1

https://synbiohub.org/public/SEGA/SEGA023/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA023/1
start: 3028
end: 4218
orientation: inline

Ubiquitin
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
codon start: 1
label: Ubiquitin
note: Ubiquitin
translation: MQIFVKTLTGKTITLEVEPGSAHHHHHHHHHHAGSSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQEKSTLELVLELQNETQSATSDA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA023/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA023/annotation7/range/1

https://synbiohub.org/public/SEGA/SEGA023/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA023/1
start: 1878
end: 2177
orientation: inline

lacO
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
bound moiety: lac repressor
feature type: protein_bind
label: lacO
note: lac operator
note: lacO
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA023/1
role: SO:0000110
location: https://synbiohub.org/public/SEGA/SEGA023/annotation5/range/1

https://synbiohub.org/public/SEGA/SEGA023/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA023/1
start: 1825
end: 1848
orientation: inline

L3S3P22 Terminator (synthetic)
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
label: L3S3P22 Terminator (synthetic)
note: L3S3P22 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA023/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA023/annotation9/range/1

https://synbiohub.org/public/SEGA/SEGA023/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA023/1
start: 2901
end: 2949
orientation: inline

https://synbiohub.org/public/SEGA/SEGA092/1
type: ComponentDefinition
persistent identity: SEGA092
display id: SEGA092
version: 1
description: natural linear DNA.
owned by: carbay
owned by: morno
date: 09-SEP-2021
division: UNA
keywords: .
locus: SEGA092
molecule: ds-DNA
organism: unspecified
reference: https://synbiohub.org/public/SEGA/reference0_1/1
source: natural DNA sequence
type: DnaRegion
type: SO:0000987
role: SO:0000804
sequence annotation: galK
sequence annotation: rhaB promoter
sequence annotation: EM7 promoter
sequence annotation: rhaS
sequence annotation: Promoter 3BCD2
sequence annotation: Terminator rrnB T1
sequence annotation: L3S2P11 Terminator
sequence annotation: pelB signal sequence
sequence annotation: glmS
sequence annotation: high TIR
sequence annotation: rrnC
sequence annotation: TcR
sequence annotation: rhaR
sequence annotation: sfGFP
sequence annotation: pstS
sequence annotation: L3S3P22 Terminator (synthetic)
sequence: https://synbiohub.org/public/SEGA/SEGA092_seq/1

galK
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
EC number: 2.7.1.6
codon start: 1
db xref: ASAP:ABE-0002568
db xref: EcoGene:EG10363
db xref: GI:16128725
db xref: GeneID:945358
db xref: UniProtKB/Swiss-Prot:P0A6T3
function: enzyme; Degradation of small molecules: Carbon compounds
gene: galK
gene synonym: ECK0746; galA; JW0740
label: galK
locus tag: b0757
note: galK
product: galactokinase
protein id: NP_415278.1
translation: MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVLPCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLDAPIVAHENYQWANYVRGVVKHLQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQEAENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTLVGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTVEAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGGGFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA092/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA092/annotation2/range/1

https://synbiohub.org/public/SEGA/SEGA092/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA092/1
start: 605
end: 1753
orientation: reverseComplement

rhaB promoter
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
label: rhaB promoter
note: promoter of the E. coli rhaBAD operon, conferring tight induction with L-rhamnose and repression with D-glucose in the presence of RhaR and RhaS (Giacalone et al., 2006)
note: rhaB promoter
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA092/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA092/annotation7/range/1

https://synbiohub.org/public/SEGA/SEGA092/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA092/1
start: 3800
end: 3918
orientation: inline

EM7 promoter
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
label: EM7 promoter
note: EM7 promoter
note: synthetic bacterial promoter
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA092/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA092/annotation3/range/1

https://synbiohub.org/public/SEGA/SEGA092/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA092/1
start: 1783
end: 1830
orientation: reverseComplement

rhaS
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
codon start: 1
gene: E. coli rhaS
label: rhaS
note: activated by L-rhamnose
note: rhaS
product: positive regulator of the rhaB promoter
translation: MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHGTGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLLPQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKSSLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQRYLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGFLQ
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA092/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA092/annotation6/range/1

https://synbiohub.org/public/SEGA/SEGA092/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA092/1
start: 2818
end: 3654
orientation: reverseComplement

Promoter 3BCD2
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
label: Promoter 3BCD2
note: Promoter 3BCD2
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA092/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA092/annotation12/range/1

https://synbiohub.org/public/SEGA/SEGA092/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA092/1
start: 4787
end: 4815
orientation: inline

Terminator rrnB T1
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
label: Terminator rrnB T1
note: Terminator rrnB T1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA092/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA092/annotation4/range/1

https://synbiohub.org/public/SEGA/SEGA092/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA092/1
start: 1843
end: 1886
orientation: inline

L3S2P11 Terminator
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
label: L3S2P11 Terminator
note: L3S2P11 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA092/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA092/annotation1/range/1

https://synbiohub.org/public/SEGA/SEGA092/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA092/1
start: 538
end: 594
orientation: inline

pelB signal sequence
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
codon start: 1
gene: pelB (fragment)
label: pelB signal sequence
note: pelB signal sequence
product: leader peptide for secretion
translation: MKYLLPTAAAGLLLLAAQPAMA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA092/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA092/annotation9/range/1

https://synbiohub.org/public/SEGA/SEGA092/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA092/1
start: 3948
end: 4013
orientation: inline

glmS
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
codon start: 1
label: glmS
note: glmS
translation: VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA092/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA092/annotation0/range/1

https://synbiohub.org/public/SEGA/SEGA092/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA092/1
start: 1
end: 528
orientation: inline

high TIR
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
note: TIR
note: high TIR
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA092/1
role: SO:0000139
location: https://synbiohub.org/public/SEGA/SEGA092/annotation8/range/1

https://synbiohub.org/public/SEGA/SEGA092/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA092/1
start: 3930
end: 3956
orientation: inline

rrnC
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
label: rrnC
note: rrnC
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA092/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA092/annotation14/range/1

https://synbiohub.org/public/SEGA/SEGA092/annotation14/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA092/1
start: 6057
end: 6097
orientation: inline

TcR
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
codon start: 1
gene: tet
label: TcR
note: TcR
note: confers resistance to tetracycline
product: tetracycline efflux protein
translation: MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHYGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA092/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA092/annotation13/range/1

https://synbiohub.org/public/SEGA/SEGA092/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA092/1
start: 4855
end: 6045
orientation: inline

rhaR
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
codon start: 1
gene: E. coli rhaR
label: rhaR
note: needed for rhamnose-dependent activation of the rhaB promoter
note: rhaR
product: transcriptional activator of rhaR and rhaS
translation: MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQGAIPGFNASAGQPHWRLGSMGMAQARQVIGQLEHESSQHVPFANEMAELLFGQLVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRHLNSQKD
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA092/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA092/annotation5/range/1

https://synbiohub.org/public/SEGA/SEGA092/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA092/1
start: 1899
end: 2744
orientation: reverseComplement

sfGFP
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
codon start: 1
label: sfGFP
note: sfGFP
product: superfolder GFP
translation: RKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDGTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEFVTAAGITHGMDELYK
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA092/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA092/annotation10/range/1

https://synbiohub.org/public/SEGA/SEGA092/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA092/1
start: 4014
end: 4724
orientation: inline

pstS
type: SequenceAnnotation
persistent identity: annotation15
display id: annotation15
version: 1
codon start: 1
label: pstS
note: pstS
translation: MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKWADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA092/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA092/annotation15/range/1

https://synbiohub.org/public/SEGA/SEGA092/annotation15/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA092/1
start: 6292
end: 6539
orientation: inline

L3S3P22 Terminator (synthetic)
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
label: L3S3P22 Terminator (synthetic)
note: L3S3P22 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA092/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA092/annotation11/range/1

https://synbiohub.org/public/SEGA/SEGA092/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA092/1
start: 4728
end: 4776
orientation: inline

https://synbiohub.org/public/SEGA/SEGA036/1
type: ComponentDefinition
persistent identity: SEGA036
display id: SEGA036
version: 1
description: natural linear DNA.
owned by: carbay
owned by: morno
date: 09-SEP-2021
division: UNA
keywords: .
locus: SEGA036
molecule: ds-DNA
organism: unspecified
reference: https://synbiohub.org/user/carbay/SEGA/SEGA036_reference0/1
source: natural DNA sequence
type: DnaRegion
type: SO:0000987
role: SO:0000804
sequence annotation: Promoter 3BCD2
sequence annotation: lacO
sequence annotation: pstS
sequence annotation: lacI
sequence annotation: trc promoter
sequence annotation: L3S3P22 Terminator (synthetic)
sequence annotation: lacI promoter
sequence annotation: glmS
sequence annotation: TcR
sequence annotation: medium TIR
sequence annotation: L3S2P11 Terminator
sequence annotation: YebFss
sequence annotation: sfGFP
sequence annotation: rrnC
sequence annotation: YebF
sequence: https://synbiohub.org/public/SEGA/SEGA036_seq/1

Promoter 3BCD2
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
label: Promoter 3BCD2
note: Promoter 3BCD2
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA036/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA036/annotation11/range/1

https://synbiohub.org/public/SEGA/SEGA036/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA036/1
start: 3005
end: 3033
orientation: inline

lacO
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
bound moiety: lac repressor
feature type: protein_bind
label: lacO
note: lac operator
note: lacO
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA036/1
role: SO:0000110
location: https://synbiohub.org/public/SEGA/SEGA036/annotation5/range/1

https://synbiohub.org/public/SEGA/SEGA036/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA036/1
start: 1825
end: 1848
orientation: inline

pstS
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
codon start: 1
label: pstS
note: pstS
translation: MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKWADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA036/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA036/annotation14/range/1

https://synbiohub.org/public/SEGA/SEGA036/annotation14/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA036/1
start: 4509
end: 4756
orientation: inline

lacI
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
codon start: 1
gene: lacI
label: lacI
note: The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG).
note: lacI
product: lac repressor
translation: MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA036/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA036/annotation2/range/1

https://synbiohub.org/public/SEGA/SEGA036/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA036/1
start: 604
end: 1686
orientation: reverseComplement

trc promoter
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
label: trc promoter
note: strong E. coli promoter; hybrid between the trp and lac UV5 promoters
note: trc promoter
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA036/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA036/annotation4/range/1

https://synbiohub.org/public/SEGA/SEGA036/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA036/1
start: 1795
end: 1824
orientation: inline

L3S3P22 Terminator (synthetic)
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
label: L3S3P22 Terminator (synthetic)
note: L3S3P22 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA036/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA036/annotation10/range/1

https://synbiohub.org/public/SEGA/SEGA036/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA036/1
start: 2946
end: 2994
orientation: inline

lacI promoter
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
gene: lacI
label: lacI promoter
note: lacI promoter
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA036/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA036/annotation3/range/1

https://synbiohub.org/public/SEGA/SEGA036/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA036/1
start: 1687
end: 1764
orientation: reverseComplement

glmS
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
codon start: 1
label: glmS
note: glmS
translation: VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA036/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA036/annotation0/range/1

https://synbiohub.org/public/SEGA/SEGA036/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA036/1
start: 1
end: 528
orientation: inline

TcR
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
codon start: 1
gene: tet
label: TcR
note: TcR
note: confers resistance to tetracycline
product: tetracycline efflux protein
translation: MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHYGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVIFGEDRFRWSATMSGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA036/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA036/annotation12/range/1

https://synbiohub.org/public/SEGA/SEGA036/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA036/1
start: 3073
end: 4263
orientation: inline

medium TIR
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
note: TIR
note: medium TIR
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA036/1
role: SO:0000139
location: https://synbiohub.org/public/SEGA/SEGA036/annotation6/range/1

https://synbiohub.org/public/SEGA/SEGA036/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA036/1
start: 1860
end: 1886
orientation: inline

L3S2P11 Terminator
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
label: L3S2P11 Terminator
note: L3S2P11 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA036/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA036/annotation1/range/1

https://synbiohub.org/public/SEGA/SEGA036/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA036/1
start: 538
end: 594
orientation: inline

YebFss
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
codon start: 1
db xref: ASAP:ABE-0006141
db xref: EcoGene:EG11807
db xref: GI:145698278
db xref: GeneID:946363
db xref: UniProtKB/Swiss-Prot:P33219
gene: yebF
gene synonym: ECK1848; JW1836
label: YebFss
locus tag: b1847
note: YebFss
product: yebF signal sequence
protein id: NP_416361.2
translation: MKKRGAFLGLLLVSACASVFA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA036/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA036/annotation7/range/1

https://synbiohub.org/public/SEGA/SEGA036/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA036/1
start: 1878
end: 1940
orientation: inline

sfGFP
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
codon start: 1
label: sfGFP
note: sfGFP
product: superfolder GFP
translation: RKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDGTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEFVTAAGITHGMDELYK
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA036/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA036/annotation9/range/1

https://synbiohub.org/public/SEGA/SEGA036/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA036/1
start: 2232
end: 2942
orientation: inline

rrnC
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
label: rrnC
note: rrnC
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA036/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA036/annotation13/range/1

https://synbiohub.org/public/SEGA/SEGA036/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA036/1
start: 4274
end: 4314
orientation: inline

YebF
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
codon start: 1
label: YebF
note: YebF
product: full size yebF protein
translation: ANNETSKSVTFPKCEDLDAAGIAASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHYQVSVDCKAGMAEYQRR
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA036/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA036/annotation8/range/1

https://synbiohub.org/public/SEGA/SEGA036/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA036/1
start: 1941
end: 2231
orientation: inline

https://synbiohub.org/public/SEGA/SEGA103/1
type: ComponentDefinition
persistent identity: SEGA103
display id: SEGA103
version: 1
description: natural linear DNA.
owned by: carbay
owned by: morno
date: 09-SEP-2021
division: UNA
keywords: .
locus: SEGA103
molecule: ds-DNA
organism: unspecified
reference: https://synbiohub.org/user/carbay/SEGA/SEGA103_reference0/1
source: natural DNA sequence
type: DnaRegion
type: SO:0000987
role: SO:0000804
sequence annotation: low TIR
sequence annotation: lacI
sequence annotation: BCD
sequence annotation: TcR
sequence annotation: T7 promoter
sequence annotation: lac operator
sequence annotation: lacI promoter
sequence annotation: L3S2P11 Terminator
sequence annotation: rrnC
sequence annotation: glmS
sequence annotation: pstS
sequence: https://synbiohub.org/public/SEGA/SEGA103_seq/1

low TIR
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
note: TIR
note: low TIR
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA103/1
role: SO:0000139
location: https://synbiohub.org/public/SEGA/SEGA103/annotation6/range/1

https://synbiohub.org/public/SEGA/SEGA103/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA103/1
start: 1850
end: 1876
orientation: inline

lacI
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
codon start: 1
gene: lacI
label: lacI
note: The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG).
note: lacI
product: lac repressor
translation: MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA103/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA103/annotation2/range/1

https://synbiohub.org/public/SEGA/SEGA103/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA103/1
start: 604
end: 1686
orientation: reverseComplement

BCD
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
codon start: 1
label: BCD
note: BCD
translation: MKAIFVLKHLNHAKEVF
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA103/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA103/annotation7/range/1

https://synbiohub.org/public/SEGA/SEGA103/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA103/1
start: 1868
end: 1923
orientation: inline

TcR
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
codon start: 1
gene: tet
label: TcR
note: TcR
note: confers resistance to tetracycline
product: tetracycline efflux protein
translation: VLPGLLRDIVHSDSIASHYGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVIFGEDRFRWSATMSGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA103/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA103/annotation8/range/1

https://synbiohub.org/public/SEGA/SEGA103/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA103/1
start: 1924
end: 3036
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
label: T7 promoter
note: T7 promoter
note: promoter for bacteriophage T7 RNA polymerase
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA103/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA103/annotation4/range/1

https://synbiohub.org/public/SEGA/SEGA103/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA103/1
start: 1795
end: 1813
orientation: inline

lac operator
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
bound moiety: lac repressor encoded by lacI
feature type: protein_bind
label: lac operator
note: The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG).
note: lac operator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA103/1
role: SO:0000110
location: https://synbiohub.org/public/SEGA/SEGA103/annotation5/range/1

https://synbiohub.org/public/SEGA/SEGA103/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA103/1
start: 1814
end: 1838
orientation: inline

lacI promoter
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
gene: lacI
label: lacI promoter
note: lacI promoter
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA103/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA103/annotation3/range/1

https://synbiohub.org/public/SEGA/SEGA103/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA103/1
start: 1687
end: 1764
orientation: reverseComplement

L3S2P11 Terminator
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
label: L3S2P11 Terminator
note: L3S2P11 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA103/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA103/annotation1/range/1

https://synbiohub.org/public/SEGA/SEGA103/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA103/1
start: 538
end: 594
orientation: inline

rrnC
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
label: rrnC
note: rrnC
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA103/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA103/annotation9/range/1

https://synbiohub.org/public/SEGA/SEGA103/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA103/1
start: 3047
end: 3087
orientation: inline

glmS
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
codon start: 1
label: glmS
note: glmS
translation: VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA103/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA103/annotation0/range/1

https://synbiohub.org/public/SEGA/SEGA103/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA103/1
start: 1
end: 528
orientation: inline

pstS
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
codon start: 1
label: pstS
note: pstS
translation: MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKWADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA103/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA103/annotation10/range/1

https://synbiohub.org/public/SEGA/SEGA103/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA103/1
start: 3282
end: 3529
orientation: inline

https://synbiohub.org/public/SEGA/SEGA046/1
type: ComponentDefinition
persistent identity: SEGA046
display id: SEGA046
version: 1
description: natural linear DNA.
owned by: carbay
owned by: morno
date: 08-SEP-2021
division: UNA
keywords: .
locus: SEGA046
molecule: ds-DNA
organism: unspecified
reference: https://synbiohub.org/user/carbay/SEGA/SEGA046_reference0/1
source: natural DNA sequence
type: DnaRegion
type: SO:0000987
role: SO:0000804
sequence annotation: rhaR
sequence annotation: rrnC
sequence annotation: glmS
sequence annotation: rhaS
sequence annotation: L3S2P11 Terminator
sequence annotation: TEV site
sequence annotation: TcR
sequence annotation: high TIR
sequence annotation: pstS
sequence annotation: L3S3P22 Terminator (synthetic)
sequence annotation: GFP
sequence annotation: Promoter 3BCD2
sequence annotation: rhaB promoter
sequence: https://synbiohub.org/public/SEGA/SEGA046_seq/1

rhaR
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
codon start: 1
gene: E. coli rhaR
label: rhaR
note: needed for rhamnose-dependent activation of the rhaB promoter
note: rhaR
product: transcriptional activator of rhaR and rhaS
translation: MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQGAIPGFNASAGQPHWRLGSMGMAQARQVIGQLEHESSQHVPFANEMAELLFGQLVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRHLNSQKD
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA046/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA046/annotation2/range/1

https://synbiohub.org/public/SEGA/SEGA046/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA046/1
start: 607
end: 1452
orientation: reverseComplement

rrnC
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
label: rrnC
note: rrnC
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA046/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA046/annotation11/range/1

https://synbiohub.org/public/SEGA/SEGA046/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA046/1
start: 4719
end: 4759
orientation: inline

glmS
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
codon start: 1
label: glmS
note: glmS
translation: VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA046/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA046/annotation0/range/1

https://synbiohub.org/public/SEGA/SEGA046/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA046/1
start: 1
end: 528
orientation: inline

rhaS
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
codon start: 1
gene: E. coli rhaS
label: rhaS
note: activated by L-rhamnose
note: rhaS
product: positive regulator of the rhaB promoter
translation: MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHGTGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLLPQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKSSLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQRYLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGFLQ
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA046/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA046/annotation3/range/1

https://synbiohub.org/public/SEGA/SEGA046/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA046/1
start: 1526
end: 2362
orientation: reverseComplement

L3S2P11 Terminator
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
label: L3S2P11 Terminator
note: L3S2P11 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA046/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA046/annotation1/range/1

https://synbiohub.org/public/SEGA/SEGA046/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA046/1
start: 538
end: 594
orientation: inline

TEV site
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
codon start: 1
label: TEV site
note: TEV site
product: tobacco etch virus (TEV) protease recognition and cleavage site
translation: MENLYFQ
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA046/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA046/annotation6/range/1

https://synbiohub.org/public/SEGA/SEGA046/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA046/1
start: 2656
end: 2676
orientation: inline

TcR
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
codon start: 1
gene: tet
label: TcR
note: TcR
note: confers resistance to tetracycline
product: tetracycline efflux protein
translation: MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHYGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVIFGEDRFRWSATMSGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA046/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA046/annotation10/range/1

https://synbiohub.org/public/SEGA/SEGA046/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA046/1
start: 3518
end: 4708
orientation: inline

high TIR
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
note: TIR
note: high TIR
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA046/1
role: SO:0000139
location: https://synbiohub.org/public/SEGA/SEGA046/annotation5/range/1

https://synbiohub.org/public/SEGA/SEGA046/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA046/1
start: 2638
end: 2664
orientation: inline

pstS
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
codon start: 1
label: pstS
note: pstS
translation: MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKWADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA046/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA046/annotation12/range/1

https://synbiohub.org/public/SEGA/SEGA046/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA046/1
start: 4954
end: 5201
orientation: inline

L3S3P22 Terminator (synthetic)
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
label: L3S3P22 Terminator (synthetic)
note: L3S3P22 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA046/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA046/annotation8/range/1

https://synbiohub.org/public/SEGA/SEGA046/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA046/1
start: 3391
end: 3439
orientation: inline

GFP
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
codon start: 1
label: GFP
note: GFP
product: green fluorescent protein
translation: SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSTLSKDPNEKRDHMVLLEFVTAAGITHGMDELYK
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA046/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA046/annotation7/range/1

https://synbiohub.org/public/SEGA/SEGA046/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA046/1
start: 2677
end: 3387
orientation: inline

Promoter 3BCD2
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
label: Promoter 3BCD2
note: Promoter 3BCD2
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA046/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA046/annotation9/range/1

https://synbiohub.org/public/SEGA/SEGA046/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA046/1
start: 3450
end: 3478
orientation: inline

rhaB promoter
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
label: rhaB promoter
note: promoter of the E. coli rhaBAD operon, conferring tight induction with L-rhamnose and repression with D-glucose in the presence of RhaR and RhaS (Giacalone et al., 2006)
note: rhaB promoter
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA046/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA046/annotation4/range/1

https://synbiohub.org/public/SEGA/SEGA046/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA046/1
start: 2508
end: 2626
orientation: inline

https://synbiohub.org/public/SEGA/SEGA034/1
type: ComponentDefinition
persistent identity: SEGA034
display id: SEGA034
version: 1
description: natural linear DNA.
owned by: carbay
owned by: morno
date: 08-SEP-2021
division: UNA
keywords: .
locus: SEGA034
molecule: ds-DNA
organism: unspecified
reference: https://synbiohub.org/user/carbay/SEGA/SEGA034_reference0/1
source: natural DNA sequence
type: DnaRegion
type: SO:0000987
role: SO:0000804
sequence annotation: L3S2P11 Terminator
sequence annotation: TcR
sequence annotation: Promoter 3BCD2
sequence annotation: rrnC
sequence annotation: glmS
sequence annotation: pstS
sequence annotation: high TIR
sequence annotation: lacO
sequence annotation: lacI
sequence annotation: sfGFP
sequence annotation: trc promoter
sequence annotation: lacI promoter
sequence annotation: pelB signal sequence
sequence annotation: L3S3P22 Terminator (synthetic)
sequence: https://synbiohub.org/public/SEGA/SEGA034_seq/1

L3S2P11 Terminator
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
label: L3S2P11 Terminator
note: L3S2P11 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA034/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA034/annotation1/range/1

https://synbiohub.org/public/SEGA/SEGA034/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA034/1
start: 538
end: 594
orientation: inline

TcR
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
codon start: 1
gene: tet
label: TcR
note: TcR
note: confers resistance to tetracycline
product: tetracycline efflux protein
translation: MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHYGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVIFGEDRFRWSATMSGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA034/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA034/annotation11/range/1

https://synbiohub.org/public/SEGA/SEGA034/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA034/1
start: 2785
end: 3975
orientation: inline

Promoter 3BCD2
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
label: Promoter 3BCD2
note: Promoter 3BCD2
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA034/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA034/annotation10/range/1

https://synbiohub.org/public/SEGA/SEGA034/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA034/1
start: 2717
end: 2745
orientation: inline

rrnC
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
label: rrnC
note: rrnC
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA034/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA034/annotation12/range/1

https://synbiohub.org/public/SEGA/SEGA034/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA034/1
start: 3986
end: 4026
orientation: inline

glmS
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
codon start: 1
label: glmS
note: glmS
translation: VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA034/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA034/annotation0/range/1

https://synbiohub.org/public/SEGA/SEGA034/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA034/1
start: 1
end: 528
orientation: inline

pstS
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
codon start: 1
label: pstS
note: pstS
translation: MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKWADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA034/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA034/annotation13/range/1

https://synbiohub.org/public/SEGA/SEGA034/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA034/1
start: 4221
end: 4468
orientation: inline

high TIR
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
note: TIR
note: high TIR
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA034/1
role: SO:0000139
location: https://synbiohub.org/public/SEGA/SEGA034/annotation6/range/1

https://synbiohub.org/public/SEGA/SEGA034/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA034/1
start: 1860
end: 1886
orientation: inline

lacO
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
bound moiety: lac repressor
feature type: protein_bind
label: lacO
note: lac operator
note: lacO
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA034/1
role: SO:0000110
location: https://synbiohub.org/public/SEGA/SEGA034/annotation5/range/1

https://synbiohub.org/public/SEGA/SEGA034/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA034/1
start: 1825
end: 1848
orientation: inline

lacI
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
codon start: 1
gene: lacI
label: lacI
note: The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG).
note: lacI
product: lac repressor
translation: MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA034/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA034/annotation2/range/1

https://synbiohub.org/public/SEGA/SEGA034/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA034/1
start: 604
end: 1686
orientation: reverseComplement

sfGFP
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
codon start: 1
label: sfGFP
note: sfGFP
product: superfolder GFP
translation: RKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDGTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEFVTAAGITHGMDELYK
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA034/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA034/annotation8/range/1

https://synbiohub.org/public/SEGA/SEGA034/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA034/1
start: 1944
end: 2654
orientation: inline

trc promoter
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
label: trc promoter
note: strong E. coli promoter; hybrid between the trp and lac UV5 promoters
note: trc promoter
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA034/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA034/annotation4/range/1

https://synbiohub.org/public/SEGA/SEGA034/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA034/1
start: 1795
end: 1824
orientation: inline

lacI promoter
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
gene: lacI
label: lacI promoter
note: lacI promoter
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA034/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA034/annotation3/range/1

https://synbiohub.org/public/SEGA/SEGA034/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA034/1
start: 1687
end: 1764
orientation: reverseComplement

pelB signal sequence
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
codon start: 1
gene: pelB (fragment)
label: pelB signal sequence
note: pelB signal sequence
product: leader peptide for secretion
translation: MKYLLPTAAAGLLLLAAQPAMA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA034/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA034/annotation7/range/1

https://synbiohub.org/public/SEGA/SEGA034/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA034/1
start: 1878
end: 1943
orientation: inline

L3S3P22 Terminator (synthetic)
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
label: L3S3P22 Terminator (synthetic)
note: L3S3P22 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA034/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA034/annotation9/range/1

https://synbiohub.org/public/SEGA/SEGA034/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA034/1
start: 2658
end: 2706
orientation: inline

https://synbiohub.org/public/SEGA/SEGA032/1
type: ComponentDefinition
persistent identity: SEGA032
display id: SEGA032
version: 1
description: natural linear DNA.
owned by: carbay
owned by: morno
date: 08-SEP-2021
division: UNA
keywords: .
locus: SEGA032
molecule: ds-DNA
organism: unspecified
reference: https://synbiohub.org/public/SEGA/reference0_1/1
source: natural DNA sequence
type: DnaRegion
type: SO:0000987
role: SO:0000804
sequence annotation: lacI
sequence annotation: TcR
sequence annotation: low TIR
sequence annotation: pstS
sequence annotation: glmS
sequence annotation: L3S2P11 Terminator
sequence annotation: L3S3P22 Terminator (synthetic)
sequence annotation: Promoter 3BCD2
sequence annotation: lacO
sequence annotation: lacI promoter
sequence annotation: sfGFP
sequence annotation: pelB signal sequence
sequence annotation: rrnC
sequence annotation: trc promoter
sequence: https://synbiohub.org/public/SEGA/SEGA032_seq/1

lacI
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
codon start: 1
gene: lacI
label: lacI
note: The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG).
note: lacI
product: lac repressor
translation: MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA032/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA032/annotation2/range/1

https://synbiohub.org/public/SEGA/SEGA032/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA032/1
start: 604
end: 1686
orientation: reverseComplement

TcR
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
codon start: 1
gene: tet
label: TcR
note: TcR
note: confers resistance to tetracycline
product: tetracycline efflux protein
translation: MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHYGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVIFGEDRFRWSATMSGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA032/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA032/annotation11/range/1

https://synbiohub.org/public/SEGA/SEGA032/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA032/1
start: 2785
end: 3975
orientation: inline

low TIR
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
note: TIR
note: low TIR
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA032/1
role: SO:0000139
location: https://synbiohub.org/public/SEGA/SEGA032/annotation6/range/1

https://synbiohub.org/public/SEGA/SEGA032/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA032/1
start: 1860
end: 1886
orientation: inline

pstS
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
codon start: 1
label: pstS
note: pstS
translation: MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKWADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA032/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA032/annotation13/range/1

https://synbiohub.org/public/SEGA/SEGA032/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA032/1
start: 4221
end: 4468
orientation: inline

glmS
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
codon start: 1
label: glmS
note: glmS
translation: VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA032/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA032/annotation0/range/1

https://synbiohub.org/public/SEGA/SEGA032/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA032/1
start: 1
end: 528
orientation: inline

L3S2P11 Terminator
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
label: L3S2P11 Terminator
note: L3S2P11 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA032/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA032/annotation1/range/1

https://synbiohub.org/public/SEGA/SEGA032/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA032/1
start: 538
end: 594
orientation: inline

L3S3P22 Terminator (synthetic)
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
label: L3S3P22 Terminator (synthetic)
note: L3S3P22 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA032/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA032/annotation9/range/1

https://synbiohub.org/public/SEGA/SEGA032/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA032/1
start: 2658
end: 2706
orientation: inline

Promoter 3BCD2
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
label: Promoter 3BCD2
note: Promoter 3BCD2
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA032/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA032/annotation10/range/1

https://synbiohub.org/public/SEGA/SEGA032/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA032/1
start: 2717
end: 2745
orientation: inline

lacO
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
bound moiety: lac represspr
feature type: protein_bind
label: lacO
note: lac operator
note: lacO
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA032/1
role: SO:0000110
location: https://synbiohub.org/public/SEGA/SEGA032/annotation5/range/1

https://synbiohub.org/public/SEGA/SEGA032/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA032/1
start: 1825
end: 1848
orientation: inline

lacI promoter
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
gene: lacI
label: lacI promoter
note: lacI promoter
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA032/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA032/annotation3/range/1

https://synbiohub.org/public/SEGA/SEGA032/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA032/1
start: 1687
end: 1764
orientation: reverseComplement

sfGFP
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
codon start: 1
label: sfGFP
note: sfGFP
product: superfolder GFP
translation: RKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDGTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEFVTAAGITHGMDELYK
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA032/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA032/annotation8/range/1

https://synbiohub.org/public/SEGA/SEGA032/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA032/1
start: 1944
end: 2654
orientation: inline

pelB signal sequence
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
codon start: 1
gene: pelB (fragment)
label: pelB signal sequence
note: pelB signal sequence
product: leader peptide for secretion
translation: MKYLLPTAAAGLLLLAAQPAMA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA032/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA032/annotation7/range/1

https://synbiohub.org/public/SEGA/SEGA032/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA032/1
start: 1878
end: 1943
orientation: inline

rrnC
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
label: rrnC
note: rrnC
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA032/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA032/annotation12/range/1

https://synbiohub.org/public/SEGA/SEGA032/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA032/1
start: 3986
end: 4026
orientation: inline

trc promoter
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
label: trc promoter
note: strong E. coli promoter; hybrid between the trp and lac UV5 promoters
note: trc promoter
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA032/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA032/annotation4/range/1

https://synbiohub.org/public/SEGA/SEGA032/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA032/1
start: 1795
end: 1824
orientation: inline

https://synbiohub.org/public/SEGA/SEGA027/1
type: ComponentDefinition
persistent identity: SEGA027
display id: SEGA027
version: 1
description: natural linear DNA.
owned by: carbay
owned by: morno
date: 08-SEP-2021
division: UNA
keywords: .
locus: SEGA027
molecule: ds-DNA
organism: unspecified
reference: https://synbiohub.org/user/carbay/SEGA/SEGA027_reference0/1
source: natural DNA sequence
type: DnaRegion
type: SO:0000987
role: SO:0000804
sequence annotation: TEV site
sequence annotation: glmS
sequence annotation: TcR
sequence annotation: lacI
sequence annotation: GFP
sequence annotation: L3S3P22 Terminator (synthetic)
sequence annotation: rrnC
sequence annotation: lacI promoter
sequence annotation: medium TIR
sequence annotation: L3S2P11 Terminator
sequence annotation: Promoter 3BCD2
sequence annotation: pstS
sequence annotation: lacO
sequence annotation: trc promoter
sequence: https://synbiohub.org/public/SEGA/SEGA027_seq/1

TEV site
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
codon start: 1
label: TEV site
note: TEV site
product: tobacco etch virus (TEV) protease recognition and cleavage site
translation: MENLYFQ
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA027/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA027/annotation7/range/1

https://synbiohub.org/public/SEGA/SEGA027/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA027/1
start: 1878
end: 1898
orientation: inline

glmS
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
codon start: 1
label: glmS
note: glmS
translation: VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA027/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA027/annotation0/range/1

https://synbiohub.org/public/SEGA/SEGA027/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA027/1
start: 1
end: 528
orientation: inline

TcR
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
codon start: 1
gene: tet
label: TcR
note: TcR
note: confers resistance to tetracycline
product: tetracycline efflux protein
translation: MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHYGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVIFGEDRFRWSATMSGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA027/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA027/annotation11/range/1

https://synbiohub.org/public/SEGA/SEGA027/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA027/1
start: 2740
end: 3930
orientation: inline

lacI
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
codon start: 1
gene: lacI
label: lacI
note: The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG).
note: lacI
product: lac repressor
translation: MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA027/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA027/annotation2/range/1

https://synbiohub.org/public/SEGA/SEGA027/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA027/1
start: 604
end: 1686
orientation: reverseComplement

GFP
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
codon start: 1
label: GFP
note: GFP
product: green fluorescent protein
translation: SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYK
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA027/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA027/annotation8/range/1

https://synbiohub.org/public/SEGA/SEGA027/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA027/1
start: 1899
end: 2609
orientation: inline

L3S3P22 Terminator (synthetic)
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
label: L3S3P22 Terminator (synthetic)
note: L3S3P22 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA027/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA027/annotation9/range/1

https://synbiohub.org/public/SEGA/SEGA027/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA027/1
start: 2613
end: 2661
orientation: inline

rrnC
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
label: rrnC
note: rrnC
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA027/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA027/annotation12/range/1

https://synbiohub.org/public/SEGA/SEGA027/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA027/1
start: 3941
end: 3981
orientation: inline

lacI promoter
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
gene: lacI
label: lacI promoter
note: lacI promoter
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA027/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA027/annotation3/range/1

https://synbiohub.org/public/SEGA/SEGA027/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA027/1
start: 1687
end: 1764
orientation: reverseComplement

medium TIR
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
note: TIR
note: medium TIR
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA027/1
role: SO:0000139
location: https://synbiohub.org/public/SEGA/SEGA027/annotation6/range/1

https://synbiohub.org/public/SEGA/SEGA027/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA027/1
start: 1860
end: 1886
orientation: inline

L3S2P11 Terminator
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
label: L3S2P11 Terminator
note: L3S2P11 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA027/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA027/annotation1/range/1

https://synbiohub.org/public/SEGA/SEGA027/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA027/1
start: 538
end: 594
orientation: inline

Promoter 3BCD2
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
label: Promoter 3BCD2
note: Promoter 3BCD2
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA027/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA027/annotation10/range/1

https://synbiohub.org/public/SEGA/SEGA027/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA027/1
start: 2672
end: 2700
orientation: inline

pstS
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
codon start: 1
label: pstS
note: pstS
translation: MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKWADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA027/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA027/annotation13/range/1

https://synbiohub.org/public/SEGA/SEGA027/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA027/1
start: 4176
end: 4423
orientation: inline

lacO
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
bound moiety: lac repressor
feature type: protein_bind
label: lacO
note: lac operator
note: lacO
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA027/1
role: SO:0000110
location: https://synbiohub.org/public/SEGA/SEGA027/annotation5/range/1

https://synbiohub.org/public/SEGA/SEGA027/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA027/1
start: 1825
end: 1848
orientation: inline

trc promoter
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
label: trc promoter
note: strong E. coli promoter; hybrid between the trp and lac UV5 promoters
note: trc promoter
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA027/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA027/annotation4/range/1

https://synbiohub.org/public/SEGA/SEGA027/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA027/1
start: 1795
end: 1824
orientation: inline

https://synbiohub.org/public/SEGA/SEGA018/1
type: ComponentDefinition
persistent identity: SEGA018
display id: SEGA018
version: 1
description: natural linear DNA.
owned by: carbay
owned by: morno
date: 09-SEP-2021
division: UNA
keywords: .
locus: SEGA018
molecule: ds-DNA
organism: unspecified
reference: https://synbiohub.org/user/carbay/SEGA/SEGA018_reference0/1
source: natural DNA sequence
type: DnaRegion
type: SO:0000987
role: SO:0000804
sequence annotation: L3S2P11 Terminator
sequence annotation: glmS
sequence annotation: pstS
sequence annotation: medium TIR
sequence annotation: sfGFP
sequence annotation: pelB signal sequence
sequence annotation: P-J23100
sequence annotation: Promoter 3BCD2
sequence annotation: L3S3P22 Terminator (synthetic)
sequence annotation: rrnC
sequence annotation: TcR
sequence: https://synbiohub.org/public/SEGA/SEGA018_seq/1

L3S2P11 Terminator
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
label: L3S2P11 Terminator
note: L3S2P11 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA018/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA018/annotation1/range/1

https://synbiohub.org/public/SEGA/SEGA018/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA018/1
start: 538
end: 594
orientation: inline

glmS
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
codon start: 1
label: glmS
note: glmS
translation: VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA018/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA018/annotation0/range/1

https://synbiohub.org/public/SEGA/SEGA018/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA018/1
start: 1
end: 528
orientation: inline

pstS
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
codon start: 1
label: pstS
note: pstS
translation: MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKWADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA018/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA018/annotation10/range/1

https://synbiohub.org/public/SEGA/SEGA018/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA018/1
start: 3011
end: 3258
orientation: inline

medium TIR
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
note: TIR
note: medium TIR
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA018/1
role: SO:0000139
location: https://synbiohub.org/public/SEGA/SEGA018/annotation3/range/1

https://synbiohub.org/public/SEGA/SEGA018/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA018/1
start: 650
end: 676
orientation: inline

sfGFP
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
codon start: 1
label: sfGFP
note: sfGFP
product: superfolder GFP
translation: RKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDGTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEFVTAAGITHGMDELYK
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA018/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA018/annotation5/range/1

https://synbiohub.org/public/SEGA/SEGA018/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA018/1
start: 734
end: 1444
orientation: inline

pelB signal sequence
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
codon start: 1
gene: pelB (fragment)
label: pelB signal sequence
note: pelB signal sequence
product: leader peptide for secretion
translation: MKYLLPTAAAGLLLLAAQPAMA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA018/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA018/annotation4/range/1

https://synbiohub.org/public/SEGA/SEGA018/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA018/1
start: 668
end: 733
orientation: inline

P-J23100
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
label: P-J23100
note: P-J23100
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA018/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA018/annotation2/range/1

https://synbiohub.org/public/SEGA/SEGA018/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA018/1
start: 604
end: 638
orientation: inline

Promoter 3BCD2
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
label: Promoter 3BCD2
note: Promoter 3BCD2
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA018/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA018/annotation7/range/1

https://synbiohub.org/public/SEGA/SEGA018/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA018/1
start: 1507
end: 1535
orientation: inline

L3S3P22 Terminator (synthetic)
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
label: L3S3P22 Terminator (synthetic)
note: L3S3P22 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA018/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA018/annotation6/range/1

https://synbiohub.org/public/SEGA/SEGA018/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA018/1
start: 1448
end: 1496
orientation: inline

rrnC
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
label: rrnC
note: rrnC
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA018/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA018/annotation9/range/1

https://synbiohub.org/public/SEGA/SEGA018/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA018/1
start: 2776
end: 2816
orientation: inline

TcR
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
codon start: 1
gene: tet
label: TcR
note: TcR
note: confers resistance to tetracycline
product: tetracycline efflux protein
translation: MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHYGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVIFGEDRFRWSATMSGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA018/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA018/annotation8/range/1

https://synbiohub.org/public/SEGA/SEGA018/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA018/1
start: 1575
end: 2765
orientation: inline

https://synbiohub.org/public/SEGA/SEGA022/1
type: ComponentDefinition
persistent identity: SEGA022
display id: SEGA022
version: 1
description: natural linear DNA.
owned by: carbay
owned by: morno
date: 09-SEP-2021
division: UNA
keywords: .
locus: SEGA022
molecule: ds-DNA
organism: unspecified
reference: https://synbiohub.org/public/SEGA/reference0_1/1
source: natural DNA sequence
type: DnaRegion
type: SO:0000987
role: SO:0000804
sequence annotation: pstS
sequence annotation: lacO
sequence annotation: Promoter 3BCD2
sequence annotation: lacI
sequence annotation: L3S3P22 Terminator (synthetic)
sequence annotation: high TIR
sequence annotation: L3S2P11 Terminator
sequence annotation: GFP
sequence annotation: glmS
sequence annotation: BCD
sequence annotation: rrnC
sequence annotation: lacI promoter
sequence annotation: trc promoter
sequence annotation: TcR
sequence: https://synbiohub.org/public/SEGA/SEGA022_seq/1

pstS
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
codon start: 1
label: pstS
note: pstS
translation: MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKWADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA022/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA022/annotation13/range/1

https://synbiohub.org/public/SEGA/SEGA022/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA022/1
start: 4211
end: 4458
orientation: inline

lacO
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
bound moiety: lac repressor
feature type: protein_bind
label: lacO
note: lac operator
note: lacO
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA022/1
role: SO:0000110
location: https://synbiohub.org/public/SEGA/SEGA022/annotation5/range/1

https://synbiohub.org/public/SEGA/SEGA022/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA022/1
start: 1825
end: 1848
orientation: inline

Promoter 3BCD2
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
label: Promoter 3BCD2
note: Promoter 3BCD2
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA022/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA022/annotation10/range/1

https://synbiohub.org/public/SEGA/SEGA022/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA022/1
start: 2707
end: 2735
orientation: inline

lacI
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
codon start: 1
gene: lacI
label: lacI
note: The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG).
note: lacI
product: lac repressor
translation: MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA022/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA022/annotation2/range/1

https://synbiohub.org/public/SEGA/SEGA022/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA022/1
start: 604
end: 1686
orientation: reverseComplement

L3S3P22 Terminator (synthetic)
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
label: L3S3P22 Terminator (synthetic)
note: L3S3P22 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA022/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA022/annotation9/range/1

https://synbiohub.org/public/SEGA/SEGA022/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA022/1
start: 2648
end: 2696
orientation: inline

high TIR
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
note: TIR
note: high TIR
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA022/1
role: SO:0000139
location: https://synbiohub.org/public/SEGA/SEGA022/annotation6/range/1

https://synbiohub.org/public/SEGA/SEGA022/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA022/1
start: 1860
end: 1886
orientation: inline

L3S2P11 Terminator
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
label: L3S2P11 Terminator
note: L3S2P11 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA022/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA022/annotation1/range/1

https://synbiohub.org/public/SEGA/SEGA022/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA022/1
start: 538
end: 594
orientation: inline

GFP
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
codon start: 1
label: GFP
note: GFP
product: green fluorescent protein
translation: SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYK
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA022/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA022/annotation8/range/1

https://synbiohub.org/public/SEGA/SEGA022/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA022/1
start: 1934
end: 2644
orientation: inline

glmS
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
codon start: 1
label: glmS
note: glmS
translation: VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA022/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA022/annotation0/range/1

https://synbiohub.org/public/SEGA/SEGA022/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA022/1
start: 1
end: 528
orientation: inline

BCD
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
codon start: 1
label: BCD
note: BCD
translation: MKAIFVLKHLNHAKEVF
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA022/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA022/annotation7/range/1

https://synbiohub.org/public/SEGA/SEGA022/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA022/1
start: 1878
end: 1933
orientation: inline

rrnC
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
label: rrnC
note: rrnC
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA022/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA022/annotation12/range/1

https://synbiohub.org/public/SEGA/SEGA022/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA022/1
start: 3976
end: 4016
orientation: inline

lacI promoter
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
gene: lacI
label: lacI promoter
note: lacI promoter
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA022/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA022/annotation3/range/1

https://synbiohub.org/public/SEGA/SEGA022/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA022/1
start: 1687
end: 1764
orientation: reverseComplement

trc promoter
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
label: trc promoter
note: strong E. coli promoter; hybrid between the trp and lac UV5 promoters
note: trc promoter
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA022/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA022/annotation4/range/1

https://synbiohub.org/public/SEGA/SEGA022/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA022/1
start: 1795
end: 1824
orientation: inline

TcR
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
codon start: 1
gene: tet
label: TcR
note: TcR
note: confers resistance to tetracycline
product: tetracycline efflux protein
translation: MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHYGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVIFGEDRFRWSATMSGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA022/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA022/annotation11/range/1

https://synbiohub.org/public/SEGA/SEGA022/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA022/1
start: 2775
end: 3965
orientation: inline

https://synbiohub.org/public/SEGA/SEGA100/1
type: ComponentDefinition
persistent identity: SEGA100
display id: SEGA100
version: 1
description: natural linear DNA.
owned by: carbay
owned by: morno
date: 09-SEP-2021
division: UNA
keywords: .
locus: SEGA100
molecule: ds-DNA
organism: unspecified
reference: https://synbiohub.org/user/carbay/SEGA/SEGA100_reference0/1
source: natural DNA sequence
type: DnaRegion
type: SO:0000987
role: SO:0000804
sequence annotation: rrnC
sequence annotation: TcR
sequence annotation: high TIR
sequence annotation: lacI promoter
sequence annotation: pstS
sequence annotation: lacO
sequence annotation: glmS
sequence annotation: L3S2P11 Terminator
sequence annotation: BCD
sequence annotation: trc promoter
sequence annotation: lacI
sequence: https://synbiohub.org/public/SEGA/SEGA100_seq/1

rrnC
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
label: rrnC
note: rrnC
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA100/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA100/annotation9/range/1

https://synbiohub.org/public/SEGA/SEGA100/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA100/1
start: 3057
end: 3097
orientation: inline

TcR
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
codon start: 1
gene: tet
label: TcR
note: TcR
note: confers resistance to tetracycline
product: tetracycline efflux protein
translation: VLPGLLRDIVHSDSIASHYGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVIFGEDRFRWSATMSGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA100/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA100/annotation8/range/1

https://synbiohub.org/public/SEGA/SEGA100/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA100/1
start: 1934
end: 3046
orientation: inline

high TIR
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
note: TIR
note: high TIR
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA100/1
role: SO:0000139
location: https://synbiohub.org/public/SEGA/SEGA100/annotation6/range/1

https://synbiohub.org/public/SEGA/SEGA100/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA100/1
start: 1860
end: 1886
orientation: inline

lacI promoter
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
gene: lacI
label: lacI promoter
note: lacI promoter
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA100/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA100/annotation3/range/1

https://synbiohub.org/public/SEGA/SEGA100/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA100/1
start: 1687
end: 1764
orientation: reverseComplement

pstS
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
codon start: 1
label: pstS
note: pstS
translation: MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKWADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA100/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA100/annotation10/range/1

https://synbiohub.org/public/SEGA/SEGA100/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA100/1
start: 3292
end: 3539
orientation: inline

lacO
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
bound moiety: lac repressor
feature type: protein_bind
label: lacO
note: lac operator
note: lacO
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA100/1
role: SO:0000110
location: https://synbiohub.org/public/SEGA/SEGA100/annotation5/range/1

https://synbiohub.org/public/SEGA/SEGA100/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA100/1
start: 1825
end: 1848
orientation: inline

glmS
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
codon start: 1
label: glmS
note: glmS
translation: VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA100/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA100/annotation0/range/1

https://synbiohub.org/public/SEGA/SEGA100/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA100/1
start: 1
end: 528
orientation: inline

L3S2P11 Terminator
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
label: L3S2P11 Terminator
note: L3S2P11 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA100/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA100/annotation1/range/1

https://synbiohub.org/public/SEGA/SEGA100/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA100/1
start: 538
end: 594
orientation: inline

BCD
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
codon start: 1
label: BCD
note: BCD
translation: MKAIFVLKHLNHAKEVF
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA100/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA100/annotation7/range/1

https://synbiohub.org/public/SEGA/SEGA100/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA100/1
start: 1878
end: 1933
orientation: inline

trc promoter
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
label: trc promoter
note: strong E. coli promoter; hybrid between the trp and lac UV5 promoters
note: trc promoter
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA100/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA100/annotation4/range/1

https://synbiohub.org/public/SEGA/SEGA100/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA100/1
start: 1795
end: 1824
orientation: inline

lacI
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
codon start: 1
gene: lacI
label: lacI
note: The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG).
note: lacI
product: lac repressor
translation: MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA100/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA100/annotation2/range/1

https://synbiohub.org/public/SEGA/SEGA100/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA100/1
start: 604
end: 1686
orientation: reverseComplement

https://synbiohub.org/public/SEGA/SEGA068/1
type: ComponentDefinition
persistent identity: SEGA068
display id: SEGA068
version: 1
description: natural linear DNA.
owned by: carbay
owned by: morno
date: 08-SEP-2021
division: UNA
keywords: .
locus: SEGA068
molecule: ds-DNA
organism: unspecified
reference: https://synbiohub.org/public/SEGA/reference0_1/1
source: natural DNA sequence
type: DnaRegion
type: SO:0000987
role: SO:0000804
sequence annotation: sfGFP
sequence annotation: Promoter 3BCD2
sequence annotation: L3S3P22 Terminator (synthetic)
sequence annotation: low TIR
sequence annotation: TcR
sequence annotation: rrnC
sequence annotation: T7 promoter
sequence annotation: pstS
sequence annotation: lac operator
sequence annotation: L3S2P11 Terminator
sequence annotation: lacI promoter
sequence annotation: pelB signal sequence
sequence annotation: glmS
sequence annotation: lacI
sequence: https://synbiohub.org/public/SEGA/SEGA068_seq/1

sfGFP
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
codon start: 1
label: sfGFP
note: sfGFP
translation: RKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDGTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEFVTAAGITHGMDELYK
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA068/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA068/annotation8/range/1

https://synbiohub.org/public/SEGA/SEGA068/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA068/1
start: 1934
end: 2644
orientation: inline

Promoter 3BCD2
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
label: Promoter 3BCD2
note: Promoter 3BCD2
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA068/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA068/annotation10/range/1

https://synbiohub.org/public/SEGA/SEGA068/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA068/1
start: 2707
end: 2735
orientation: inline

L3S3P22 Terminator (synthetic)
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
label: L3S3P22 Terminator (synthetic)
note: L3S3P22 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA068/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA068/annotation9/range/1

https://synbiohub.org/public/SEGA/SEGA068/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA068/1
start: 2648
end: 2696
orientation: inline

low TIR
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
note: TIR
note: low TIR
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA068/1
role: SO:0000139
location: https://synbiohub.org/public/SEGA/SEGA068/annotation6/range/1

https://synbiohub.org/public/SEGA/SEGA068/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA068/1
start: 1850
end: 1876
orientation: inline

TcR
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
codon start: 1
gene: tet
label: TcR
note: TcR
note: confers resistance to tetracycline
product: tetracycline efflux protein
translation: MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHYGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVIFGEDRFRWSATMSGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA068/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA068/annotation11/range/1

https://synbiohub.org/public/SEGA/SEGA068/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA068/1
start: 2775
end: 3965
orientation: inline

rrnC
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
label: rrnC
note: rrnC
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA068/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA068/annotation12/range/1

https://synbiohub.org/public/SEGA/SEGA068/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA068/1
start: 3976
end: 4016
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
label: T7 promoter
note: T7 promoter
note: promoter for bacteriophage T7 RNA polymerase
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA068/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA068/annotation4/range/1

https://synbiohub.org/public/SEGA/SEGA068/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA068/1
start: 1795
end: 1813
orientation: inline

pstS
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
codon start: 1
label: pstS
note: pstS
translation: MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKWADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA068/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA068/annotation13/range/1

https://synbiohub.org/public/SEGA/SEGA068/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA068/1
start: 4211
end: 4458
orientation: inline

lac operator
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
bound moiety: lac repressor encoded by lacI
feature type: protein_bind
label: lac operator
note: The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG).
note: lac operator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA068/1
role: SO:0000110
location: https://synbiohub.org/public/SEGA/SEGA068/annotation5/range/1

https://synbiohub.org/public/SEGA/SEGA068/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA068/1
start: 1814
end: 1838
orientation: inline

L3S2P11 Terminator
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
label: L3S2P11 Terminator
note: L3S2P11 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA068/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA068/annotation1/range/1

https://synbiohub.org/public/SEGA/SEGA068/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA068/1
start: 538
end: 594
orientation: inline

lacI promoter
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
gene: lacI
label: lacI promoter
note: lacI promoter
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA068/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA068/annotation3/range/1

https://synbiohub.org/public/SEGA/SEGA068/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA068/1
start: 1687
end: 1764
orientation: reverseComplement

pelB signal sequence
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
codon start: 1
gene: pelB (fragment)
label: pelB signal sequence
note: pelB signal sequence
product: leader peptide for secretion
translation: MKYLLPTAAAGLLLLAAQPAMA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA068/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA068/annotation7/range/1

https://synbiohub.org/public/SEGA/SEGA068/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA068/1
start: 1868
end: 1933
orientation: inline

glmS
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
codon start: 1
label: glmS
note: glmS
translation: VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA068/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA068/annotation0/range/1

https://synbiohub.org/public/SEGA/SEGA068/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA068/1
start: 1
end: 528
orientation: inline

lacI
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
codon start: 1
gene: lacI
label: lacI
note: The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG).
note: lacI
product: lac repressor
translation: MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA068/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA068/annotation2/range/1

https://synbiohub.org/public/SEGA/SEGA068/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA068/1
start: 604
end: 1686
orientation: reverseComplement

https://synbiohub.org/public/SEGA/SEGA076/1
type: ComponentDefinition
persistent identity: SEGA076
display id: SEGA076
version: 1
description: natural linear DNA.
owned by: carbay
owned by: morno
date: 09-SEP-2021
division: UNA
keywords: .
locus: SEGA076
molecule: ds-DNA
organism: unspecified
reference: https://synbiohub.org/public/SEGA/reference0_1/1
source: natural DNA sequence
type: DnaRegion
type: SO:0000987
role: SO:0000804
sequence annotation: L3S2P11 Terminator
sequence annotation: L3S3P22 Terminator (synthetic)
sequence annotation: TetR
sequence annotation: pstS
sequence annotation: RiboJ
sequence annotation: rrnC
sequence annotation: glmS
sequence annotation: Promoter 3BCD2
sequence annotation: low TIR
sequence annotation: tet1
sequence annotation: pTet*
sequence annotation: TcR
sequence annotation: pLacI
sequence annotation: GFP
sequence annotation: BCD
sequence: https://synbiohub.org/public/SEGA/SEGA076_seq/1

L3S2P11 Terminator
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
label: L3S2P11 Terminator
note: L3S2P11 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA076/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA076/annotation1/range/1

https://synbiohub.org/public/SEGA/SEGA076/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA076/1
start: 538
end: 594
orientation: inline

L3S3P22 Terminator (synthetic)
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
label: L3S3P22 Terminator (synthetic)
note: L3S3P22 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA076/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA076/annotation10/range/1

https://synbiohub.org/public/SEGA/SEGA076/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA076/1
start: 2293
end: 2341
orientation: inline

TetR
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
label: TetR
note: Geneious type: CDS
note: TetR
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA076/1
role: SO:0001411
location: https://synbiohub.org/public/SEGA/SEGA076/annotation2/range/1

https://synbiohub.org/public/SEGA/SEGA076/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA076/1
start: 611
end: 1234
orientation: reverseComplement

pstS
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
codon start: 1
label: pstS
note: pstS
translation: MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKWADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA076/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA076/annotation14/range/1

https://synbiohub.org/public/SEGA/SEGA076/annotation14/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA076/1
start: 3856
end: 4103
orientation: inline

RiboJ
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
label: RiboJ
note: RiboJ
note: insulator: self-cleaving ribozymes RiboJ
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA076/1
role: SO:0001411
location: https://synbiohub.org/public/SEGA/SEGA076/annotation6/range/1

https://synbiohub.org/public/SEGA/SEGA076/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA076/1
start: 1419
end: 1493
orientation: inline

rrnC
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
label: rrnC
note: rrnC
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA076/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA076/annotation13/range/1

https://synbiohub.org/public/SEGA/SEGA076/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA076/1
start: 3621
end: 3661
orientation: inline

glmS
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
codon start: 1
label: glmS
note: glmS
translation: VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA076/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA076/annotation0/range/1

https://synbiohub.org/public/SEGA/SEGA076/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA076/1
start: 1
end: 528
orientation: inline

Promoter 3BCD2
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
label: Promoter 3BCD2
note: Promoter 3BCD2
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA076/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA076/annotation11/range/1

https://synbiohub.org/public/SEGA/SEGA076/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA076/1
start: 2352
end: 2380
orientation: inline

low TIR
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
note: TIR
note: low TIR
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA076/1
role: SO:0000139
location: https://synbiohub.org/public/SEGA/SEGA076/annotation7/range/1

https://synbiohub.org/public/SEGA/SEGA076/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA076/1
start: 1505
end: 1531
orientation: inline

tet1
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
label: tet1
note: tet1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA076/1
role: SO:0000139
location: https://synbiohub.org/public/SEGA/SEGA076/annotation3/range/1

https://synbiohub.org/public/SEGA/SEGA076/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA076/1
start: 1235
end: 1262
orientation: inline

pTet*
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
label: pTet*
note: pTet*
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA076/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA076/annotation5/range/1

https://synbiohub.org/public/SEGA/SEGA076/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA076/1
start: 1343
end: 1418
orientation: inline

TcR
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
codon start: 1
gene: tet
label: TcR
note: TcR
note: confers resistance to tetracycline
product: tetracycline efflux protein
translation: MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHYGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVIFGEDRFRWSATMSGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA076/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA076/annotation12/range/1

https://synbiohub.org/public/SEGA/SEGA076/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA076/1
start: 2420
end: 3610
orientation: inline

pLacI
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
label: pLacI
note: pLacI
note: promoter
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA076/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA076/annotation4/range/1

https://synbiohub.org/public/SEGA/SEGA076/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA076/1
start: 1263
end: 1316
orientation: reverseComplement

GFP
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
codon start: 1
label: GFP
note: GFP
product: green fluorescent protein
translation: SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYK
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA076/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA076/annotation9/range/1

https://synbiohub.org/public/SEGA/SEGA076/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA076/1
start: 1579
end: 2289
orientation: inline

BCD
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
codon start: 1
label: BCD
note: BCD
translation: MKAIFVLKHLNHAKEVF
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA076/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA076/annotation8/range/1

https://synbiohub.org/public/SEGA/SEGA076/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA076/1
start: 1523
end: 1578
orientation: inline

https://synbiohub.org/public/SEGA/SEGA078/1
type: ComponentDefinition
persistent identity: SEGA078
display id: SEGA078
version: 1
description: natural linear DNA.
owned by: carbay
owned by: morno
date: 09-SEP-2021
division: UNA
keywords: .
locus: SEGA078
molecule: ds-DNA
organism: unspecified
reference: https://synbiohub.org/public/SEGA/reference0_1/1
source: natural DNA sequence
type: DnaRegion
type: SO:0000987
role: SO:0000804
sequence annotation: rhaR
sequence annotation: L3S3P22
sequence annotation: GFP
sequence annotation: rhaB promoter
sequence annotation: Promoter 3BCD2
sequence annotation: Terminator rrnB T1
sequence annotation: low TIR
sequence annotation: glmS
sequence annotation: EM7 promoter
sequence annotation: BCD
sequence annotation: L3S2P11 Terminator
sequence annotation: rhaS
sequence annotation: TcR
sequence annotation: pstS
sequence annotation: rrnC
sequence annotation: galK
sequence: https://synbiohub.org/public/SEGA/SEGA078_seq/1

rhaR
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
codon start: 1
gene: E. coli rhaR
label: rhaR
note: needed for rhamnose-dependent activation of the rhaB promoter
note: rhaR
product: transcriptional activator of rhaR and rhaS
translation: MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQGAIPGFNASAGQPHWRLGSMGMAQARQVIGQLEHESSQHVPFANEMAELLFGQLVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRHLNSQKD
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA078/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA078/annotation5/range/1

https://synbiohub.org/public/SEGA/SEGA078/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA078/1
start: 1899
end: 2744
orientation: reverseComplement

L3S3P22
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
label: L3S3P22
note: L3S3P22
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA078/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA078/annotation11/range/1

https://synbiohub.org/public/SEGA/SEGA078/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA078/1
start: 4718
end: 4766
orientation: inline

GFP
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
codon start: 1
label: GFP
note: GFP
product: green fluorescent protein
translation: SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYK
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA078/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA078/annotation10/range/1

https://synbiohub.org/public/SEGA/SEGA078/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA078/1
start: 4004
end: 4714
orientation: inline

rhaB promoter
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
label: rhaB promoter
note: promoter of the E. coli rhaBAD operon, conferring tight induction with L-rhamnose and repression with D-glucose in the presence of RhaR and RhaS (Giacalone et al., 2006)
note: rhaB promoter
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA078/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA078/annotation7/range/1

https://synbiohub.org/public/SEGA/SEGA078/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA078/1
start: 3800
end: 3918
orientation: inline

Promoter 3BCD2
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
label: Promoter 3BCD2
note: Promoter 3BCD2
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA078/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA078/annotation12/range/1

https://synbiohub.org/public/SEGA/SEGA078/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA078/1
start: 4777
end: 4805
orientation: inline

Terminator rrnB T1
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
label: Terminator rrnB T1
label: rrnB
note: rrnB
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA078/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA078/annotation4/range/1

https://synbiohub.org/public/SEGA/SEGA078/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA078/1
start: 1843
end: 1886
orientation: inline

low TIR
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
note: TIR
note: low TIR
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA078/1
role: SO:0000139
location: https://synbiohub.org/public/SEGA/SEGA078/annotation8/range/1

https://synbiohub.org/public/SEGA/SEGA078/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA078/1
start: 3930
end: 3956
orientation: inline

glmS
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
codon start: 1
label: glmS
note: glmS
translation: VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA078/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA078/annotation0/range/1

https://synbiohub.org/public/SEGA/SEGA078/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA078/1
start: 1
end: 528
orientation: inline

EM7 promoter
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
label: EM7 promoter
note: EM7 promoter
note: synthetic bacterial promoter
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA078/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA078/annotation3/range/1

https://synbiohub.org/public/SEGA/SEGA078/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA078/1
start: 1783
end: 1830
orientation: reverseComplement

BCD
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
codon start: 1
label: BCD
note: BCD
translation: MKAIFVLKHLNHAKEVF
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA078/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA078/annotation9/range/1

https://synbiohub.org/public/SEGA/SEGA078/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA078/1
start: 3948
end: 4003
orientation: inline

L3S2P11 Terminator
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
label: L3S2P11 Terminator
note: L3S2P11 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA078/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA078/annotation1/range/1

https://synbiohub.org/public/SEGA/SEGA078/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA078/1
start: 538
end: 594
orientation: inline

rhaS
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
codon start: 1
gene: E. coli rhaS
label: rhaS
note: activated by L-rhamnose
note: rhaS
product: positive regulator of the rhaB promoter
translation: MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHGTGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLLPQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKSSLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQRYLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGFLQ
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA078/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA078/annotation6/range/1

https://synbiohub.org/public/SEGA/SEGA078/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA078/1
start: 2818
end: 3654
orientation: reverseComplement

TcR
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
codon start: 1
gene: tet
label: TcR
note: TcR
note: confers resistance to tetracycline
product: tetracycline efflux protein
translation: MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHYGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVIFGEDRFRWSATMSGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA078/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA078/annotation13/range/1

https://synbiohub.org/public/SEGA/SEGA078/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA078/1
start: 4845
end: 6035
orientation: inline

pstS
type: SequenceAnnotation
persistent identity: annotation15
display id: annotation15
version: 1
codon start: 1
label: pstS
note: pstS
translation: MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKWADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA078/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA078/annotation15/range/1

https://synbiohub.org/public/SEGA/SEGA078/annotation15/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA078/1
start: 6281
end: 6528
orientation: inline

rrnC
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
label: rrnC
note: rrnC
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA078/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA078/annotation14/range/1

https://synbiohub.org/public/SEGA/SEGA078/annotation14/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA078/1
start: 6046
end: 6086
orientation: inline

galK
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
EC number: 2.7.1.6
codon start: 1
db xref: ASAP:ABE-0002568
db xref: EcoGene:EG10363
db xref: GI:16128725
db xref: GeneID:945358
db xref: UniProtKB/Swiss-Prot:P0A6T3
function: enzyme; Degradation of small molecules: Carbon compounds
gene: galK
gene synonym: ECK0746; galA; JW0740
label: galK
locus tag: b0757
note: galK
product: galactokinase
protein id: NP_415278.1
translation: MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVLPCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLDAPIVAHENYQWANYVRGVVKHLQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQEAENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTLVGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTVEAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGGGFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETLYVCKPSQGAGQC
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA078/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA078/annotation2/range/1

https://synbiohub.org/public/SEGA/SEGA078/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA078/1
start: 605
end: 1753
orientation: reverseComplement

https://synbiohub.org/public/SEGA/SEGA042/1
type: ComponentDefinition
persistent identity: SEGA042
display id: SEGA042
version: 1
description: natural linear DNA.
owned by: carbay
owned by: morno
date: 08-SEP-2021
division: UNA
keywords: .
locus: SEGA042
molecule: ds-DNA
organism: unspecified
reference: https://synbiohub.org/user/carbay/SEGA/SEGA042_reference0/1
source: natural DNA sequence
type: DnaRegion
type: SO:0000987
role: SO:0000804
sequence annotation: medium TIR
sequence annotation: GFP
sequence annotation: pstS
sequence annotation: L3S3P22 Terminator (synthetic)
sequence annotation: rrnC
sequence annotation: glmS
sequence annotation: L3S2P11 Terminator
sequence annotation: rhaR
sequence annotation: rhaB promoter
sequence annotation: rhaS
sequence annotation: Promoter 3BCD2
sequence annotation: TcR
sequence annotation: Ubiquitin
sequence: https://synbiohub.org/public/SEGA/SEGA042_seq/1

medium TIR
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
note: TIR
note: medium TIR
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA042/1
role: SO:0000139
location: https://synbiohub.org/public/SEGA/SEGA042/annotation5/range/1

https://synbiohub.org/public/SEGA/SEGA042/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA042/1
start: 2638
end: 2664
orientation: inline

GFP
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
codon start: 1
label: GFP
note: GFP
product: green fluorescent protein
translation: SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYK
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA042/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA042/annotation7/range/1

https://synbiohub.org/public/SEGA/SEGA042/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA042/1
start: 2965
end: 3675
orientation: inline

pstS
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
codon start: 1
label: pstS
note: pstS
translation: MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKWADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA042/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA042/annotation12/range/1

https://synbiohub.org/public/SEGA/SEGA042/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA042/1
start: 5242
end: 5489
orientation: inline

L3S3P22 Terminator (synthetic)
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
label: L3S3P22 Terminator (synthetic)
note: L3S3P22 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA042/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA042/annotation8/range/1

https://synbiohub.org/public/SEGA/SEGA042/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA042/1
start: 3679
end: 3727
orientation: inline

rrnC
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
label: rrnC
note: rrnC
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA042/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA042/annotation11/range/1

https://synbiohub.org/public/SEGA/SEGA042/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA042/1
start: 5007
end: 5047
orientation: inline

glmS
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
codon start: 1
label: glmS
note: glmS
translation: VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA042/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA042/annotation0/range/1

https://synbiohub.org/public/SEGA/SEGA042/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA042/1
start: 1
end: 528
orientation: inline

L3S2P11 Terminator
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
label: L3S2P11 Terminator
note: L3S2P11 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA042/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA042/annotation1/range/1

https://synbiohub.org/public/SEGA/SEGA042/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA042/1
start: 538
end: 594
orientation: inline

rhaR
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
codon start: 1
gene: E. coli rhaR
label: rhaR
note: needed for rhamnose-dependent activation of the rhaB promoter
note: rhaR
product: transcriptional activator of rhaR and rhaS
translation: MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQGAIPGFNASAGQPHWRLGSMGMAQARQVIGQLEHESSQHVPFANEMAELLFGQLVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRHLNSQKD
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA042/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA042/annotation2/range/1

https://synbiohub.org/public/SEGA/SEGA042/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA042/1
start: 607
end: 1452
orientation: reverseComplement

rhaB promoter
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
label: rhaB promoter
note: promoter of the E. coli rhaBAD operon, conferring tight induction with L-rhamnose and repression with D-glucose in the presence of RhaR and RhaS (Giacalone et al., 2006)
note: rhaB promoter
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA042/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA042/annotation4/range/1

https://synbiohub.org/public/SEGA/SEGA042/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA042/1
start: 2508
end: 2626
orientation: inline

rhaS
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
codon start: 1
gene: E. coli rhaS
label: rhaS
note: activated by L-rhamnose
note: rhaS
product: positive regulator of the rhaB promoter
translation: MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHGTGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLLPQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKSSLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQRYLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGFLQ
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA042/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA042/annotation3/range/1

https://synbiohub.org/public/SEGA/SEGA042/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA042/1
start: 1526
end: 2362
orientation: reverseComplement

Promoter 3BCD2
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
label: Promoter 3BCD2
note: Promoter 3BCD2
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA042/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA042/annotation9/range/1

https://synbiohub.org/public/SEGA/SEGA042/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA042/1
start: 3738
end: 3766
orientation: inline

TcR
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
codon start: 1
gene: tet
label: TcR
note: TcR
note: confers resistance to tetracycline
product: tetracycline efflux protein
translation: MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHYGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVIFGEDRFRWSATMSGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA042/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA042/annotation10/range/1

https://synbiohub.org/public/SEGA/SEGA042/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA042/1
start: 3806
end: 4996
orientation: inline

Ubiquitin
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
codon start: 1
label: Ubiquitin
note: Ubiquitin
translation: MQIFVKTLTGKTITLEVEPGSAHHHHHHHHHHAGSSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQEKSTLELVLELQNETQSATSDA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA042/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA042/annotation6/range/1

https://synbiohub.org/public/SEGA/SEGA042/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA042/1
start: 2656
end: 2955
orientation: inline

https://synbiohub.org/public/SEGA/SEGA067/1
type: ComponentDefinition
persistent identity: SEGA067
display id: SEGA067
version: 1
description: natural linear DNA.
owned by: carbay
owned by: morno
date: 08-SEP-2021
division: UNA
keywords: .
locus: SEGA067
molecule: ds-DNA
organism: unspecified
reference: https://synbiohub.org/public/SEGA/reference0_1/1
source: natural DNA sequence
type: DnaRegion
type: SO:0000987
role: SO:0000804
sequence annotation: lacI
sequence annotation: lacI promoter
sequence annotation: L3S3P22 Terminator (synthetic)
sequence annotation: high TIR
sequence annotation: lac operator
sequence annotation: Promoter 3BCD2
sequence annotation: TEV site
sequence annotation: glmS
sequence annotation: L3S2P11 Terminator
sequence annotation: T7 promoter
sequence annotation: pstS
sequence annotation: GFP
sequence annotation: rrnC
sequence annotation: PROTi-Linker
sequence annotation: TcR
sequence: https://synbiohub.org/public/SEGA/SEGA067_seq/1

lacI
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
codon start: 1
gene: lacI
label: lacI
note: The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG).
note: lacI
product: lac repressor
translation: MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA067/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA067/annotation2/range/1

https://synbiohub.org/public/SEGA/SEGA067/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA067/1
start: 604
end: 1686
orientation: reverseComplement

lacI promoter
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
gene: lacI
label: lacI promoter
note: lacI promoter
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA067/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA067/annotation3/range/1

https://synbiohub.org/public/SEGA/SEGA067/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA067/1
start: 1687
end: 1764
orientation: reverseComplement

L3S3P22 Terminator (synthetic)
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
label: L3S3P22 Terminator (synthetic)
note: L3S3P22 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA067/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA067/annotation10/range/1

https://synbiohub.org/public/SEGA/SEGA067/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA067/1
start: 2639
end: 2687
orientation: inline

high TIR
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
note: TIR
note: high TIR
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA067/1
role: SO:0000139
location: https://synbiohub.org/public/SEGA/SEGA067/annotation6/range/1

https://synbiohub.org/public/SEGA/SEGA067/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA067/1
start: 1850
end: 1876
orientation: inline

lac operator
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
bound moiety: lac repressor encoded by lacI
feature type: protein_bind
label: lac operator
note: The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG).
note: lac operator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA067/1
role: SO:0000110
location: https://synbiohub.org/public/SEGA/SEGA067/annotation5/range/1

https://synbiohub.org/public/SEGA/SEGA067/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA067/1
start: 1814
end: 1838
orientation: inline

Promoter 3BCD2
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
label: Promoter 3BCD2
note: Promoter 3BCD2
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA067/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA067/annotation11/range/1

https://synbiohub.org/public/SEGA/SEGA067/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA067/1
start: 2698
end: 2726
orientation: inline

TEV site
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
codon start: 1
label: TEV site
note: TEV site
product: tobacco etch virus (TEV) protease recognition and cleavage site
translation: MENLYFQ
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA067/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA067/annotation7/range/1

https://synbiohub.org/public/SEGA/SEGA067/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA067/1
start: 1868
end: 1888
orientation: inline

glmS
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
codon start: 1
label: glmS
note: glmS
translation: VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA067/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA067/annotation0/range/1

https://synbiohub.org/public/SEGA/SEGA067/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA067/1
start: 1
end: 528
orientation: inline

L3S2P11 Terminator
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
label: L3S2P11 Terminator
note: L3S2P11 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA067/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA067/annotation1/range/1

https://synbiohub.org/public/SEGA/SEGA067/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA067/1
start: 538
end: 594
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
label: T7 promoter
note: T7 promoter
note: promoter for bacteriophage T7 RNA polymerase
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA067/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA067/annotation4/range/1

https://synbiohub.org/public/SEGA/SEGA067/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA067/1
start: 1795
end: 1813
orientation: inline

pstS
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
codon start: 1
label: pstS
note: pstS
translation: MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKWADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA067/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA067/annotation14/range/1

https://synbiohub.org/public/SEGA/SEGA067/annotation14/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA067/1
start: 4202
end: 4449
orientation: inline

GFP
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
codon start: 1
label: GFP
note: GFP
product: green fluorescent protein
translation: SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYK
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA067/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA067/annotation9/range/1

https://synbiohub.org/public/SEGA/SEGA067/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA067/1
start: 1925
end: 2635
orientation: inline

rrnC
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
label: rrnC
note: rrnC
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA067/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA067/annotation13/range/1

https://synbiohub.org/public/SEGA/SEGA067/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA067/1
start: 3967
end: 4007
orientation: inline

PROTi-Linker
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
label: PROTi-Linker
note: PROTi-Linker
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA067/1
role: SO:0001411
location: https://synbiohub.org/public/SEGA/SEGA067/annotation8/range/1

https://synbiohub.org/public/SEGA/SEGA067/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA067/1
start: 1889
end: 1924
orientation: inline

TcR
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
codon start: 1
gene: tet
label: TcR
note: TcR
note: confers resistance to tetracycline
product: tetracycline efflux protein
translation: MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHYGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVIFGEDRFRWSATMSGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA067/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA067/annotation12/range/1

https://synbiohub.org/public/SEGA/SEGA067/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA067/1
start: 2766
end: 3956
orientation: inline

https://synbiohub.org/public/SEGA/SEGA008/1
type: ComponentDefinition
persistent identity: SEGA008
display id: SEGA008
version: 1
description: natural linear DNA.
owned by: carbay
owned by: morno
date: 09-SEP-2021
division: UNA
keywords: .
locus: SEGA008
molecule: ds-DNA
organism: unspecified
reference: https://synbiohub.org/user/carbay/SEGA/SEGA008_reference0/1
source: natural DNA sequence
type: DnaRegion
type: SO:0000987
role: SO:0000804
sequence annotation: Promoter 3BCD2
sequence annotation: pstS
sequence annotation: Ubiquitin
sequence annotation: TcR
sequence annotation: GFP
sequence annotation: P-J23100
sequence annotation: low TIR
sequence annotation: L3S2P11 Terminator
sequence annotation: glmS
sequence annotation: L3S3P22 Terminator (synthetic)
sequence annotation: rrnC
sequence: https://synbiohub.org/public/SEGA/SEGA008_seq/1

Promoter 3BCD2
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
label: Promoter 3BCD2
note: Promoter 3BCD2
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA008/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA008/annotation7/range/1

https://synbiohub.org/public/SEGA/SEGA008/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA008/1
start: 1750
end: 1778
orientation: inline

pstS
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
codon start: 1
label: pstS
note: pstS
translation: MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKWADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA008/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA008/annotation10/range/1

https://synbiohub.org/public/SEGA/SEGA008/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA008/1
start: 3254
end: 3501
orientation: inline

Ubiquitin
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
codon start: 1
label: Ubiquitin
note: Ubiquitin
translation: MQIFVKTLTGKTITLEVEPGSAHHHHHHHHHHAGSSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQEKSTLELVLELQNETQSATSDA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA008/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA008/annotation4/range/1

https://synbiohub.org/public/SEGA/SEGA008/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA008/1
start: 668
end: 967
orientation: inline

TcR
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
codon start: 1
gene: tet
label: TcR
note: TcR
note: confers resistance to tetracycline
product: tetracycline efflux protein
translation: MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHYGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVIFGEDRFRWSATMSGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA008/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA008/annotation8/range/1

https://synbiohub.org/public/SEGA/SEGA008/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA008/1
start: 1818
end: 3008
orientation: inline

GFP
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
codon start: 1
label: GFP
note: GFP
product: green fluorescent protein
translation: SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYK
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA008/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA008/annotation5/range/1

https://synbiohub.org/public/SEGA/SEGA008/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA008/1
start: 977
end: 1687
orientation: inline

P-J23100
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
label: P-J23100
note: P-J23100
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA008/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA008/annotation2/range/1

https://synbiohub.org/public/SEGA/SEGA008/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA008/1
start: 604
end: 638
orientation: inline

low TIR
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
note: TIR
note: low TIR
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA008/1
role: SO:0000139
location: https://synbiohub.org/public/SEGA/SEGA008/annotation3/range/1

https://synbiohub.org/public/SEGA/SEGA008/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA008/1
start: 650
end: 676
orientation: inline

L3S2P11 Terminator
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
label: L3S2P11 Terminator
note: L3S2P11 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA008/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA008/annotation1/range/1

https://synbiohub.org/public/SEGA/SEGA008/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA008/1
start: 538
end: 594
orientation: inline

glmS
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
codon start: 1
label: glmS
note: glmS
translation: VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA008/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA008/annotation0/range/1

https://synbiohub.org/public/SEGA/SEGA008/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA008/1
start: 1
end: 528
orientation: inline

L3S3P22 Terminator (synthetic)
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
label: L3S3P22 Terminator (synthetic)
note: L3S3P22 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA008/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA008/annotation6/range/1

https://synbiohub.org/public/SEGA/SEGA008/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA008/1
start: 1691
end: 1739
orientation: inline

rrnC
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
label: rrnC
note: rrnC
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA008/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA008/annotation9/range/1

https://synbiohub.org/public/SEGA/SEGA008/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA008/1
start: 3019
end: 3059
orientation: inline

https://synbiohub.org/public/SEGA/SEGA007/1
type: ComponentDefinition
persistent identity: SEGA007
display id: SEGA007
version: 1
description: natural linear DNA.
owned by: carbay
owned by: morno
date: 09-SEP-2021
division: UNA
keywords: .
locus: SEGA007
molecule: ds-DNA
organism: unspecified
reference: https://synbiohub.org/user/carbay/SEGA/SEGA007_reference0/1
source: natural DNA sequence
type: DnaRegion
type: SO:0000987
role: SO:0000804
sequence annotation: TcR
sequence annotation: rrnC
sequence annotation: L3S2P11 Terminator
sequence annotation: L3S3P22 Terminator (synthetic)
sequence annotation: GFP
sequence annotation: glmS
sequence annotation: Promoter 3BCD2
sequence annotation: pstS
sequence annotation: high TIR
sequence annotation: P-J23100
sequence annotation: BCD
sequence: https://synbiohub.org/public/SEGA/SEGA007_seq/1

TcR
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
codon start: 1
gene: tet
label: TcR
note: TcR
note: confers resistance to tetracycline
product: tetracycline efflux protein
translation: MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHYGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVIFGEDRFRWSATMSGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA007/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA007/annotation8/range/1

https://synbiohub.org/public/SEGA/SEGA007/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA007/1
start: 1565
end: 2755
orientation: inline

rrnC
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
label: rrnC
note: rrnC
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA007/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA007/annotation9/range/1

https://synbiohub.org/public/SEGA/SEGA007/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA007/1
start: 2766
end: 2806
orientation: inline

L3S2P11 Terminator
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
label: L3S2P11 Terminator
note: L3S2P11 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA007/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA007/annotation1/range/1

https://synbiohub.org/public/SEGA/SEGA007/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA007/1
start: 538
end: 594
orientation: inline

L3S3P22 Terminator (synthetic)
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
label: L3S3P22 Terminator (synthetic)
note: L3S3P22 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA007/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA007/annotation6/range/1

https://synbiohub.org/public/SEGA/SEGA007/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA007/1
start: 1438
end: 1486
orientation: inline

GFP
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
codon start: 1
label: GFP
note: GFP
product: green fluorescent protein
translation: SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYK
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA007/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA007/annotation5/range/1

https://synbiohub.org/public/SEGA/SEGA007/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA007/1
start: 724
end: 1434
orientation: inline

glmS
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
codon start: 1
label: glmS
note: glmS
translation: VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA007/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA007/annotation0/range/1

https://synbiohub.org/public/SEGA/SEGA007/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA007/1
start: 1
end: 528
orientation: inline

Promoter 3BCD2
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
label: Promoter 3BCD2
note: Promoter 3BCD2
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA007/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA007/annotation7/range/1

https://synbiohub.org/public/SEGA/SEGA007/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA007/1
start: 1497
end: 1525
orientation: inline

pstS
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
codon start: 1
label: pstS
note: pstS
translation: MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKWADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA007/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA007/annotation10/range/1

https://synbiohub.org/public/SEGA/SEGA007/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA007/1
start: 3001
end: 3248
orientation: inline

high TIR
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
note: TIR
note: high TIR
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA007/1
role: SO:0000139
location: https://synbiohub.org/public/SEGA/SEGA007/annotation3/range/1

https://synbiohub.org/public/SEGA/SEGA007/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA007/1
start: 650
end: 676
orientation: inline

P-J23100
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
label: P-J23100
note: P-J23100
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA007/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA007/annotation2/range/1

https://synbiohub.org/public/SEGA/SEGA007/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA007/1
start: 604
end: 638
orientation: inline

BCD
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
codon start: 1
label: BCD
note: BCD
translation: MKAIFVLKHLNHAKEVF
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA007/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA007/annotation4/range/1

https://synbiohub.org/public/SEGA/SEGA007/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA007/1
start: 668
end: 723
orientation: inline

https://synbiohub.org/public/SEGA/SEGA045/1
type: ComponentDefinition
persistent identity: SEGA045
display id: SEGA045
version: 1
description: natural linear DNA.
owned by: carbay
owned by: morno
date: 08-SEP-2021
division: UNA
keywords: .
locus: SEGA045
molecule: ds-DNA
organism: unspecified
reference: https://synbiohub.org/user/carbay/SEGA/SEGA045_reference0/1
source: natural DNA sequence
type: DnaRegion
type: SO:0000987
role: SO:0000804
sequence annotation: rhaB promoter
sequence annotation: rhaS
sequence annotation: TEV site
sequence annotation: Promoter 3BCD2
sequence annotation: glmS
sequence annotation: rrnC
sequence annotation: TcR
sequence annotation: medium TIR
sequence annotation: L3S3P22 Terminator (synthetic)
sequence annotation: GFP
sequence annotation: rhaR
sequence annotation: pstS
sequence annotation: L3S2P11 Terminator
sequence: https://synbiohub.org/public/SEGA/SEGA045_seq/1

rhaB promoter
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
label: rhaB promoter
note: promoter of the E. coli rhaBAD operon, conferring tight induction with L-rhamnose and repression with D-glucose in the presence of RhaR and RhaS (Giacalone et al., 2006)
note: rhaB promoter
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA045/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA045/annotation4/range/1

https://synbiohub.org/public/SEGA/SEGA045/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA045/1
start: 2508
end: 2626
orientation: inline

rhaS
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
codon start: 1
gene: E. coli rhaS
label: rhaS
note: activated by L-rhamnose
note: rhaS
product: positive regulator of the rhaB promoter
translation: MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHGTGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLLPQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKSSLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQRYLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGFLQ
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA045/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA045/annotation3/range/1

https://synbiohub.org/public/SEGA/SEGA045/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA045/1
start: 1526
end: 2362
orientation: reverseComplement

TEV site
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
codon start: 1
label: TEV site
note: TEV site
product: tobacco etch virus (TEV) protease recognition and cleavage site
translation: MENLYFQ
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA045/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA045/annotation6/range/1

https://synbiohub.org/public/SEGA/SEGA045/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA045/1
start: 2656
end: 2676
orientation: inline

Promoter 3BCD2
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
label: Promoter 3BCD2
note: Promoter 3BCD2
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA045/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA045/annotation9/range/1

https://synbiohub.org/public/SEGA/SEGA045/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA045/1
start: 3450
end: 3478
orientation: inline

glmS
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
codon start: 1
label: glmS
note: glmS
translation: VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA045/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA045/annotation0/range/1

https://synbiohub.org/public/SEGA/SEGA045/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA045/1
start: 1
end: 528
orientation: inline

rrnC
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
label: rrnC
note: rrnC
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA045/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA045/annotation11/range/1

https://synbiohub.org/public/SEGA/SEGA045/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA045/1
start: 4719
end: 4759
orientation: inline

TcR
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
codon start: 1
gene: tet
label: TcR
note: TcR
note: confers resistance to tetracycline
product: tetracycline efflux protein
translation: MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHYGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVIFGEDRFRWSATMSGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA045/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA045/annotation10/range/1

https://synbiohub.org/public/SEGA/SEGA045/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA045/1
start: 3518
end: 4708
orientation: inline

medium TIR
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
note: TIR
note: medium TIR
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA045/1
role: SO:0000139
location: https://synbiohub.org/public/SEGA/SEGA045/annotation5/range/1

https://synbiohub.org/public/SEGA/SEGA045/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA045/1
start: 2638
end: 2664
orientation: inline

L3S3P22 Terminator (synthetic)
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
label: L3S3P22 Terminator (synthetic)
note: L3S3P22 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA045/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA045/annotation8/range/1

https://synbiohub.org/public/SEGA/SEGA045/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA045/1
start: 3391
end: 3439
orientation: inline

GFP
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
codon start: 1
label: GFP
note: GFP
product: greeen fluorescent protein
translation: SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYK
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA045/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA045/annotation7/range/1

https://synbiohub.org/public/SEGA/SEGA045/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA045/1
start: 2677
end: 3387
orientation: inline

rhaR
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
codon start: 1
gene: E. coli rhaR
label: rhaR
note: needed for rhamnose-dependent activation of the rhaB promoter
note: rhaR
product: transcriptional activator of rhaR and rhaS
translation: MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQGAIPGFNASAGQPHWRLGSMGMAQARQVIGQLEHESSQHVPFANEMAELLFGQLVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRHLNSQKD
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA045/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA045/annotation2/range/1

https://synbiohub.org/public/SEGA/SEGA045/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA045/1
start: 607
end: 1452
orientation: reverseComplement

pstS
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
codon start: 1
label: pstS
note: pstS
translation: MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKWADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA045/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA045/annotation12/range/1

https://synbiohub.org/public/SEGA/SEGA045/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA045/1
start: 4954
end: 5201
orientation: inline

L3S2P11 Terminator
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
label: L3S2P11 Terminator
note: L3S2P11 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA045/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA045/annotation1/range/1

https://synbiohub.org/public/SEGA/SEGA045/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA045/1
start: 538
end: 594
orientation: inline

https://synbiohub.org/public/SEGA/SEGA026/1
type: ComponentDefinition
persistent identity: SEGA026
display id: SEGA026
version: 1
description: natural linear DNA.
owned by: carbay
owned by: morno
date: 09-SEP-2021
division: UNA
keywords: .
locus: SEGA026
molecule: ds-DNA
organism: unspecified
reference: https://synbiohub.org/user/carbay/SEGA/SEGA026_reference0/1
source: natural DNA sequence
type: DnaRegion
type: SO:0000987
role: SO:0000804
sequence annotation: lacO
sequence annotation: TEV site
sequence annotation: lacI
sequence annotation: rrnC
sequence annotation: trc promoter
sequence annotation: GFP
sequence annotation: lacI promoter
sequence annotation: L3S2P11 Terminator
sequence annotation: low TIR
sequence annotation: Promoter 3BCD2
sequence annotation: L3S3P22 Terminator (synthetic)
sequence annotation: TcR
sequence annotation: glmS
sequence annotation: pstS
sequence: https://synbiohub.org/public/SEGA/SEGA026_seq/1

lacO
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
bound moiety: lac repressor
feature type: protein_bind
label: lacO
note: lac operator
note: lacO
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA026/1
role: SO:0000110
location: https://synbiohub.org/public/SEGA/SEGA026/annotation5/range/1

https://synbiohub.org/public/SEGA/SEGA026/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA026/1
start: 1825
end: 1848
orientation: inline

TEV site
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
codon start: 1
label: TEV site
note: TEV site
product: tobacco etch virus (TEV) protease recognition and cleavage site
translation: MENLYFQ
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA026/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA026/annotation7/range/1

https://synbiohub.org/public/SEGA/SEGA026/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA026/1
start: 1878
end: 1898
orientation: inline

lacI
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
codon start: 1
gene: lacI
label: lacI
note: The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG).
note: lacI
product: lac repressor
translation: MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA026/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA026/annotation2/range/1

https://synbiohub.org/public/SEGA/SEGA026/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA026/1
start: 604
end: 1686
orientation: reverseComplement

rrnC
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
label: rrnC
note: rrnC
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA026/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA026/annotation12/range/1

https://synbiohub.org/public/SEGA/SEGA026/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA026/1
start: 3941
end: 3981
orientation: inline

trc promoter
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
label: trc promoter
note: strong E. coli promoter; hybrid between the trp and lac UV5 promoters
note: trc promoter
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA026/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA026/annotation4/range/1

https://synbiohub.org/public/SEGA/SEGA026/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA026/1
start: 1795
end: 1824
orientation: inline

GFP
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
codon start: 1
label: GFP
note: GFP
product: green fluorescent protein
translation: SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYK
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA026/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA026/annotation8/range/1

https://synbiohub.org/public/SEGA/SEGA026/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA026/1
start: 1899
end: 2609
orientation: inline

lacI promoter
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
gene: lacI
label: lacI promoter
note: lacI promoter
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA026/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA026/annotation3/range/1

https://synbiohub.org/public/SEGA/SEGA026/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA026/1
start: 1687
end: 1764
orientation: reverseComplement

L3S2P11 Terminator
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
label: L3S2P11 Terminator
note: L3S2P11 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA026/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA026/annotation1/range/1

https://synbiohub.org/public/SEGA/SEGA026/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA026/1
start: 538
end: 594
orientation: inline

low TIR
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
note: TIR
note: low TIR
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA026/1
role: SO:0000139
location: https://synbiohub.org/public/SEGA/SEGA026/annotation6/range/1

https://synbiohub.org/public/SEGA/SEGA026/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA026/1
start: 1862
end: 1886
orientation: inline

Promoter 3BCD2
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
label: Promoter 3BCD2
note: Promoter 3BCD2
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA026/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA026/annotation10/range/1

https://synbiohub.org/public/SEGA/SEGA026/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA026/1
start: 2672
end: 2700
orientation: inline

L3S3P22 Terminator (synthetic)
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
label: L3S3P22 Terminator (synthetic)
note: L3S3P22 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA026/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA026/annotation9/range/1

https://synbiohub.org/public/SEGA/SEGA026/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA026/1
start: 2613
end: 2661
orientation: inline

TcR
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
codon start: 1
gene: tet
label: TcR
note: TcR
note: confers resistance to tetracycline
product: tetracycline efflux protein
translation: MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHYGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVIFGEDRFRWSATMSGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA026/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA026/annotation11/range/1

https://synbiohub.org/public/SEGA/SEGA026/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA026/1
start: 2740
end: 3930
orientation: inline

glmS
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
codon start: 1
label: glmS
note: glmS
translation: VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA026/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA026/annotation0/range/1

https://synbiohub.org/public/SEGA/SEGA026/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA026/1
start: 1
end: 528
orientation: inline

pstS
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
codon start: 1
label: pstS
note: pstS
translation: MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKWADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA026/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA026/annotation13/range/1

https://synbiohub.org/public/SEGA/SEGA026/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA026/1
start: 4176
end: 4423
orientation: inline

https://synbiohub.org/public/SEGA/SEGA030/1
type: ComponentDefinition
persistent identity: SEGA030
display id: SEGA030
version: 1
description: natural linear DNA.
owned by: carbay
owned by: morno
date: 08-SEP-2021
division: UNA
keywords: .
locus: SEGA030
molecule: ds-DNA
organism: unspecified
reference: https://synbiohub.org/user/carbay/SEGA/SEGA030_reference0/1
source: natural DNA sequence
type: DnaRegion
type: SO:0000987
role: SO:0000804
sequence annotation: L3S2P11 Terminator
sequence annotation: PROTi-Linker
sequence annotation: medium TIR
sequence annotation: pstS
sequence annotation: lacI
sequence annotation: TEV site
sequence annotation: Promoter 3BCD2
sequence annotation: lacI promoter
sequence annotation: rrnC
sequence annotation: TcR
sequence annotation: glmS
sequence annotation: The trc promoter combines the -35 region from the trp promoter and the -10 region from the lacUV5 promoter
sequence annotation: L3S3P22 Terminator (synthetic)
sequence annotation: lacO
sequence annotation: GFP
sequence: https://synbiohub.org/public/SEGA/SEGA030_seq/1

L3S2P11 Terminator
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
label: L3S2P11 Terminator
note: L3S2P11 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA030/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA030/annotation1/range/1

https://synbiohub.org/public/SEGA/SEGA030/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA030/1
start: 538
end: 594
orientation: inline

PROTi-Linker
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
label: PROTi-Linker
note: PROTi-Linker
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA030/1
role: SO:0001411
location: https://synbiohub.org/public/SEGA/SEGA030/annotation8/range/1

https://synbiohub.org/public/SEGA/SEGA030/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA030/1
start: 1899
end: 1934
orientation: inline

medium TIR
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
note: TIR
note: medium TIR
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA030/1
role: SO:0000139
location: https://synbiohub.org/public/SEGA/SEGA030/annotation6/range/1

https://synbiohub.org/public/SEGA/SEGA030/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA030/1
start: 1860
end: 1886
orientation: inline

pstS
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
codon start: 1
label: pstS
note: pstS
translation: MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKWADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA030/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA030/annotation14/range/1

https://synbiohub.org/public/SEGA/SEGA030/annotation14/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA030/1
start: 4212
end: 4459
orientation: inline

lacI
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
codon start: 1
gene: lacI
label: lacI
note: The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG).
note: lacI
product: lac repressor
translation: MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA030/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA030/annotation2/range/1

https://synbiohub.org/public/SEGA/SEGA030/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA030/1
start: 604
end: 1686
orientation: reverseComplement

TEV site
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
codon start: 1
label: TEV site
note: TEV site
product: tobacco etch virus (TEV) protease recognition and cleavage site
translation: MENLYFQ
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA030/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA030/annotation7/range/1

https://synbiohub.org/public/SEGA/SEGA030/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA030/1
start: 1878
end: 1898
orientation: inline

Promoter 3BCD2
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
label: Promoter 3BCD2
note: Promoter 3BCD2
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA030/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA030/annotation11/range/1

https://synbiohub.org/public/SEGA/SEGA030/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA030/1
start: 2708
end: 2736
orientation: inline

lacI promoter
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
gene: lacI
label: lacI promoter
note: lacI promoter
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA030/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA030/annotation3/range/1

https://synbiohub.org/public/SEGA/SEGA030/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA030/1
start: 1687
end: 1764
orientation: reverseComplement

rrnC
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
label: rrnC
note: rrnC
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA030/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA030/annotation13/range/1

https://synbiohub.org/public/SEGA/SEGA030/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA030/1
start: 3977
end: 4017
orientation: inline

TcR
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
codon start: 1
gene: tet
label: TcR
note: TcR
note: confers resistance to tetracycline
product: tetracycline efflux protein
translation: MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHYGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVIFGEDRFRWSATMSGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA030/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA030/annotation12/range/1

https://synbiohub.org/public/SEGA/SEGA030/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA030/1
start: 2776
end: 3966
orientation: inline

glmS
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
codon start: 1
label: glmS
note: glmS
translation: VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA030/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA030/annotation0/range/1

https://synbiohub.org/public/SEGA/SEGA030/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA030/1
start: 1
end: 528
orientation: inline

The trc promoter combines the -35 region from the trp promoter and the -10 region from the lacUV5 promoter
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
note: Ptrc
note: The trc promoter combines the -35 region from the trp promoter and the -10 region from the lacUV5 promoter
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA030/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA030/annotation4/range/1

https://synbiohub.org/public/SEGA/SEGA030/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA030/1
start: 1795
end: 1824
orientation: inline

L3S3P22 Terminator (synthetic)
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
label: L3S3P22 Terminator (synthetic)
note: L3S3P22 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA030/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA030/annotation10/range/1

https://synbiohub.org/public/SEGA/SEGA030/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA030/1
start: 2649
end: 2697
orientation: inline

lacO
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
bound moiety: lac repressor
feature type: protein_bind
label: lacO
note: lacO
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA030/1
role: SO:0000110
location: https://synbiohub.org/public/SEGA/SEGA030/annotation5/range/1

https://synbiohub.org/public/SEGA/SEGA030/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA030/1
start: 1825
end: 1848
orientation: inline

GFP
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
codon start: 1
label: GFP
note: GFP
product: green fluorescent protein
translation: SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYK
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA030/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA030/annotation9/range/1

https://synbiohub.org/public/SEGA/SEGA030/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA030/1
start: 1935
end: 2645
orientation: inline

https://synbiohub.org/public/SEGA/SEGA021/1
type: ComponentDefinition
persistent identity: SEGA021
display id: SEGA021
version: 1
description: natural linear DNA.
owned by: carbay
owned by: morno
date: 08-SEP-2021
division: UNA
keywords: .
locus: SEGA021
molecule: ds-DNA
organism: unspecified
reference: https://synbiohub.org/public/SEGA/reference0_1/1
source: natural DNA sequence
type: DnaRegion
type: SO:0000987
role: SO:0000804
sequence annotation: lacI
sequence annotation: medium TIR
sequence annotation: L3S3P22 Terminator (synthetic)
sequence annotation: Promoter 3BCD2
sequence annotation: trc promoter
sequence annotation: rrnC
sequence annotation: BCD
sequence annotation: pstS
sequence annotation: lacI promoter
sequence annotation: GFP
sequence annotation: lacO
sequence annotation: L3S2P11 Terminator
sequence annotation: TcR
sequence annotation: glmS
sequence: https://synbiohub.org/public/SEGA/SEGA021_seq/1

https://synbiohub.org/public/SEGA/reference0_1/1
type: Reference
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA021/1
top level: https://synbiohub.org/public/SEGA/SEGA022/1
authors: Morten Norholm Group
journal: Exported Sep 9, 2021 from SnapGene 4.2.11 http://www.snapgene.com
label: 1 (bases 1 to 4458)
title: Direct Submission

lacI
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
codon start: 1
gene: lacI
label: lacI
note: The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG).
note: lacI
product: lac repressor
translation: MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA021/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA021/annotation2/range/1

https://synbiohub.org/public/SEGA/SEGA021/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA021/1
start: 604
end: 1686
orientation: reverseComplement

medium TIR
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
note: TIR
note: medium TIR
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA021/1
role: SO:0000139
location: https://synbiohub.org/public/SEGA/SEGA021/annotation6/range/1

https://synbiohub.org/public/SEGA/SEGA021/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA021/1
start: 1860
end: 1886
orientation: inline

L3S3P22 Terminator (synthetic)
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
label: L3S3P22 Terminator (synthetic)
note: L3S3P22 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA021/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA021/annotation9/range/1

https://synbiohub.org/public/SEGA/SEGA021/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA021/1
start: 2648
end: 2696
orientation: inline

Promoter 3BCD2
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
label: Promoter 3BCD2
note: Promoter 3BCD2
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA021/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA021/annotation10/range/1

https://synbiohub.org/public/SEGA/SEGA021/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA021/1
start: 2707
end: 2735
orientation: inline

trc promoter
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
label: trc promoter
note: strong E. coli promoter; hybrid between the trp and lac UV5 promoters
note: trc promoter
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA021/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA021/annotation4/range/1

https://synbiohub.org/public/SEGA/SEGA021/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA021/1
start: 1795
end: 1824
orientation: inline

rrnC
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
label: rrnC
note: rrnC
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA021/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA021/annotation12/range/1

https://synbiohub.org/public/SEGA/SEGA021/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA021/1
start: 3976
end: 4016
orientation: inline

BCD
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
codon start: 1
label: BCD
note: BCD
translation: MKAIFVLKHLNHAKEVF
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA021/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA021/annotation7/range/1

https://synbiohub.org/public/SEGA/SEGA021/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA021/1
start: 1878
end: 1933
orientation: inline

pstS
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
codon start: 1
label: pstS
note: pstS
translation: MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKWADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA021/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA021/annotation13/range/1

https://synbiohub.org/public/SEGA/SEGA021/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA021/1
start: 4211
end: 4458
orientation: inline

lacI promoter
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
gene: lacI
label: lacI promoter
note: lacI promoter
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA021/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA021/annotation3/range/1

https://synbiohub.org/public/SEGA/SEGA021/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA021/1
start: 1687
end: 1764
orientation: reverseComplement

GFP
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
codon start: 1
label: GFP
note: GFP
product: green fluorescent protein
translation: SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYK
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA021/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA021/annotation8/range/1

https://synbiohub.org/public/SEGA/SEGA021/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA021/1
start: 1934
end: 2644
orientation: inline

lacO
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
bound moiety: lac repressor
feature type: protein_bind
label: lacO
note: lac operator
note: lacO
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA021/1
role: SO:0000110
location: https://synbiohub.org/public/SEGA/SEGA021/annotation5/range/1

https://synbiohub.org/public/SEGA/SEGA021/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA021/1
start: 1825
end: 1848
orientation: inline

L3S2P11 Terminator
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
label: L3S2P11 Terminator
note: L3S2P11 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA021/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA021/annotation1/range/1

https://synbiohub.org/public/SEGA/SEGA021/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA021/1
start: 538
end: 594
orientation: inline

TcR
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
codon start: 1
gene: tet
label: TcR
note: TcR
note: confers resistance to tetracycline
product: tetracycline efflux protein
translation: MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHYGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVIFGEDRFRWSATMSGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA021/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA021/annotation11/range/1

https://synbiohub.org/public/SEGA/SEGA021/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA021/1
start: 2775
end: 3965
orientation: inline

glmS
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
codon start: 1
label: glmS
note: glmS
translation: VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA021/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA021/annotation0/range/1

https://synbiohub.org/public/SEGA/SEGA021/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA021/1
start: 1
end: 528
orientation: inline

https://synbiohub.org/public/SEGA/SEGA084/1
type: ComponentDefinition
persistent identity: SEGA084
display id: SEGA084
version: 1
description: natural linear DNA.
owned by: carbay
owned by: morno
date: 09-SEP-2021
division: UNA
keywords: .
locus: SEGA084
molecule: ds-DNA
organism: unspecified
reference: https://synbiohub.org/user/carbay/SEGA/SEGA084_reference0/1
source: natural DNA sequence
type: DnaRegion
type: SO:0000987
role: SO:0000804
sequence annotation: low TIR
sequence annotation: TEV site
sequence annotation: pstS
sequence annotation: L3S3P22
sequence annotation: Terminator rrnB T1
sequence annotation: rhaS
sequence annotation: glmS
sequence annotation: L3S2P11 Terminator
sequence annotation: rhaB promoter
sequence annotation: rrnC
sequence annotation: rhaR
sequence annotation: TcR
sequence annotation: Promoter 3BCD2
sequence annotation: GFP
sequence annotation: EM7 promoter
sequence annotation: galK
sequence: https://synbiohub.org/public/SEGA/SEGA084_seq/1

low TIR
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
note: TIR
note: low TIR
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA084/1
role: SO:0000139
location: https://synbiohub.org/public/SEGA/SEGA084/annotation8/range/1

https://synbiohub.org/public/SEGA/SEGA084/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA084/1
start: 3930
end: 3956
orientation: inline

TEV site
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
codon start: 1
label: TEV site
note: TEV site
product: tobacco etch virus (TEV) protease recognition and cleavage site
translation: MENLYFQ
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA084/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA084/annotation9/range/1

https://synbiohub.org/public/SEGA/SEGA084/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA084/1
start: 3948
end: 3968
orientation: inline

pstS
type: SequenceAnnotation
persistent identity: annotation15
display id: annotation15
version: 1
codon start: 1
label: pstS
note: pstS
translation: MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKWADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA084/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA084/annotation15/range/1

https://synbiohub.org/public/SEGA/SEGA084/annotation15/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA084/1
start: 6246
end: 6493
orientation: inline

L3S3P22
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
label: L3S3P22
note: L3S3P22 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA084/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA084/annotation11/range/1

https://synbiohub.org/public/SEGA/SEGA084/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA084/1
start: 4683
end: 4731
orientation: inline

Terminator rrnB T1
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
label: Terminator rrnB T1
note: Terminator rrnB T1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA084/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA084/annotation4/range/1

https://synbiohub.org/public/SEGA/SEGA084/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA084/1
start: 1843
end: 1886
orientation: inline

rhaS
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
codon start: 1
gene: E. coli rhaS
label: rhaS
note: activated by L-rhamnose
note: rhaS
product: positive regulator of the rhaB promoter
translation: MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHGTGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLLPQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKSSLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQRYLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGFLQ
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA084/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA084/annotation6/range/1

https://synbiohub.org/public/SEGA/SEGA084/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA084/1
start: 2818
end: 3654
orientation: reverseComplement

glmS
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
codon start: 1
label: glmS
note: glmS
translation: VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA084/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA084/annotation0/range/1

https://synbiohub.org/public/SEGA/SEGA084/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA084/1
start: 1
end: 528
orientation: inline

L3S2P11 Terminator
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
label: L3S2P11 Terminator
note: L3S2P11 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA084/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA084/annotation1/range/1

https://synbiohub.org/public/SEGA/SEGA084/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA084/1
start: 538
end: 594
orientation: inline

rhaB promoter
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
label: rhaB promoter
note: promoter of the E. coli rhaBAD operon, conferring tight induction with L-rhamnose and repression with D-glucose in the presence of RhaR and RhaS (Giacalone et al., 2006)
note: rhaB promoter
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA084/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA084/annotation7/range/1

https://synbiohub.org/public/SEGA/SEGA084/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA084/1
start: 3800
end: 3918
orientation: inline

rrnC
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
label: rrnC
note: rrnC
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA084/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA084/annotation14/range/1

https://synbiohub.org/public/SEGA/SEGA084/annotation14/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA084/1
start: 6011
end: 6051
orientation: inline

rhaR
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
codon start: 1
gene: E. coli rhaR
label: rhaR
note: needed for rhamnose-dependent activation of the rhaB promoter
note: rhaR
product: transcriptional activator of rhaR and rhaS
translation: MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQGAIPGFNASAGQPHWRLGSMGMAQARQVIGQLEHESSQHVPFANEMAELLFGQLVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRHLNSQKD
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA084/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA084/annotation5/range/1

https://synbiohub.org/public/SEGA/SEGA084/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA084/1
start: 1899
end: 2744
orientation: reverseComplement

TcR
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
codon start: 1
gene: tet
label: TcR
note: TcR
note: confers resistance to tetracycline
product: tetracycline efflux protein
translation: MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHYGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA084/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA084/annotation13/range/1

https://synbiohub.org/public/SEGA/SEGA084/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA084/1
start: 4810
end: 6000
orientation: inline

Promoter 3BCD2
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
label: Promoter 3BCD2
note: Promoter 3BCD2
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA084/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA084/annotation12/range/1

https://synbiohub.org/public/SEGA/SEGA084/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA084/1
start: 4742
end: 4770
orientation: inline

GFP
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
codon start: 1
label: GFP
note: GFP
product: green fluorescent protein
translation: SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYK
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA084/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA084/annotation10/range/1

https://synbiohub.org/public/SEGA/SEGA084/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA084/1
start: 3969
end: 4679
orientation: inline

EM7 promoter
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
label: EM7 promoter
note: EM7 promoter
note: synthetic bacterial promoter
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA084/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA084/annotation3/range/1

https://synbiohub.org/public/SEGA/SEGA084/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA084/1
start: 1783
end: 1830
orientation: reverseComplement

galK
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
EC number: 2.7.1.6
codon start: 1
db xref: ASAP:ABE-0002568
db xref: EcoGene:EG10363
db xref: GI:16128725
db xref: GeneID:945358
db xref: UniProtKB/Swiss-Prot:P0A6T3
function: enzyme; Degradation of small molecules: Carbon compounds
gene: galK
gene synonym: ECK0746; galA; JW0740
label: galK
locus tag: b0757
note: galK
product: galactokinase
protein id: NP_415278.1
translation: MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVLPCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLDAPIVAHENYQWANYVRGVVKHLQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQEAENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTLVGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTVEAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGGGFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA084/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA084/annotation2/range/1

https://synbiohub.org/public/SEGA/SEGA084/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA084/1
start: 605
end: 1753
orientation: reverseComplement

https://synbiohub.org/public/SEGA/SEGA016/1
type: ComponentDefinition
persistent identity: SEGA016
display id: SEGA016
version: 1
description: natural linear DNA.
owned by: carbay
owned by: morno
date: 09-SEP-2021
division: UNA
keywords: .
locus: SEGA016
molecule: ds-DNA
organism: unspecified
reference: https://synbiohub.org/user/carbay/SEGA/SEGA016_reference0/1
source: natural DNA sequence
type: DnaRegion
type: SO:0000987
role: SO:0000804
sequence annotation: PROTi-Linker
sequence annotation: L3S3P22 Terminator (synthetic)
sequence annotation: pstS
sequence annotation: TEV site
sequence annotation: high TIR
sequence annotation: P-J23100
sequence annotation: GFP
sequence annotation: glmS
sequence annotation: L3S2P11 Terminator
sequence annotation: rrnC
sequence annotation: Promoter 3BCD2
sequence annotation: TcR
sequence: https://synbiohub.org/public/SEGA/SEGA016_seq/1

PROTi-Linker
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
label: PROTi-Linker
note: PROTi-Linker
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA016/1
role: SO:0001411
location: https://synbiohub.org/public/SEGA/SEGA016/annotation5/range/1

https://synbiohub.org/public/SEGA/SEGA016/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA016/1
start: 689
end: 724
orientation: inline

L3S3P22 Terminator (synthetic)
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
label: L3S3P22 Terminator (synthetic)
note: L3S3P22 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA016/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA016/annotation7/range/1

https://synbiohub.org/public/SEGA/SEGA016/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA016/1
start: 1439
end: 1487
orientation: inline

pstS
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
codon start: 1
label: pstS
note: pstS
translation: MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKWADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA016/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA016/annotation11/range/1

https://synbiohub.org/public/SEGA/SEGA016/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA016/1
start: 3002
end: 3249
orientation: inline

TEV site
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
codon start: 1
label: TEV site
note: TEV site
product: tobacco etch virus (TEV) protease recognition and cleavage site
translation: MENLYFQ
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA016/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA016/annotation4/range/1

https://synbiohub.org/public/SEGA/SEGA016/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA016/1
start: 668
end: 688
orientation: inline

high TIR
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
note: TIR
note: high TIR
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA016/1
role: SO:0000139
location: https://synbiohub.org/public/SEGA/SEGA016/annotation3/range/1

https://synbiohub.org/public/SEGA/SEGA016/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA016/1
start: 650
end: 676
orientation: inline

P-J23100
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
label: P-J23100
note: P-J23100
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA016/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA016/annotation2/range/1

https://synbiohub.org/public/SEGA/SEGA016/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA016/1
start: 604
end: 638
orientation: inline

GFP
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
codon start: 1
label: GFP
note: GFP
product: green fluorescent protein
translation: SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYK
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA016/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA016/annotation6/range/1

https://synbiohub.org/public/SEGA/SEGA016/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA016/1
start: 725
end: 1435
orientation: inline

glmS
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
codon start: 1
label: glmS
note: glmS
translation: VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA016/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA016/annotation0/range/1

https://synbiohub.org/public/SEGA/SEGA016/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA016/1
start: 1
end: 528
orientation: inline

L3S2P11 Terminator
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
label: L3S2P11 Terminator
note: L3S2P11 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA016/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA016/annotation1/range/1

https://synbiohub.org/public/SEGA/SEGA016/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA016/1
start: 538
end: 594
orientation: inline

rrnC
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
label: rrnC
note: rrnC
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA016/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA016/annotation10/range/1

https://synbiohub.org/public/SEGA/SEGA016/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA016/1
start: 2767
end: 2807
orientation: inline

Promoter 3BCD2
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
label: Promoter 3BCD2
note: Promoter 3BCD2
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA016/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA016/annotation8/range/1

https://synbiohub.org/public/SEGA/SEGA016/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA016/1
start: 1498
end: 1526
orientation: inline

TcR
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
codon start: 1
gene: tet
label: TcR
note: TcR
note: confers resistance to tetracycline
product: tetracycline efflux protein
translation: MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHYGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVIFGEDRFRWSATMSGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA016/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA016/annotation9/range/1

https://synbiohub.org/public/SEGA/SEGA016/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA016/1
start: 1566
end: 2756
orientation: inline

https://synbiohub.org/public/SEGA/SEGA064/1
type: ComponentDefinition
persistent identity: SEGA064
display id: SEGA064
version: 1
description: natural linear DNA.
owned by: carbay
owned by: morno
date: 08-SEP-2021
division: UNA
keywords: .
locus: SEGA064
molecule: ds-DNA
organism: unspecified
reference: https://synbiohub.org/public/SEGA/reference0_1/1
source: natural DNA sequence
type: DnaRegion
type: SO:0000987
role: SO:0000804
sequence annotation: L3S3P22 Terminator (synthetic)
sequence annotation: L3S2P11 Terminator
sequence annotation: pstS
sequence annotation: T7 promoter
sequence annotation: GFP
sequence annotation: TEV site
sequence annotation: lacI
sequence annotation: lacI promoter
sequence annotation: glmS
sequence annotation: Promoter 3BCD2
sequence annotation: lac operator
sequence annotation: high TIR
sequence annotation: rrnC
sequence annotation: TcR
sequence: https://synbiohub.org/public/SEGA/SEGA064_seq/1

L3S3P22 Terminator (synthetic)
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
label: L3S3P22 Terminator (synthetic)
note: L3S3P22 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA064/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA064/annotation9/range/1

https://synbiohub.org/public/SEGA/SEGA064/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA064/1
start: 2603
end: 2651
orientation: inline

L3S2P11 Terminator
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
label: L3S2P11 Terminator
note: L3S2P11 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA064/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA064/annotation1/range/1

https://synbiohub.org/public/SEGA/SEGA064/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA064/1
start: 538
end: 594
orientation: inline

pstS
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
codon start: 1
label: pstS
note: pstS
translation: MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKWADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA064/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA064/annotation13/range/1

https://synbiohub.org/public/SEGA/SEGA064/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA064/1
start: 4166
end: 4413
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
label: T7 promoter
note: T7 promoter
note: promoter for bacteriophage T7 RNA polymerase
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA064/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA064/annotation4/range/1

https://synbiohub.org/public/SEGA/SEGA064/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA064/1
start: 1795
end: 1813
orientation: inline

GFP
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
codon start: 1
label: GFP
note: GFP
product: green fluorescent protein
translation: SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSTLSKDPNEKRDHMVLLEFVTAAGITHGMDELYK
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA064/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA064/annotation8/range/1

https://synbiohub.org/public/SEGA/SEGA064/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA064/1
start: 1889
end: 2599
orientation: inline

TEV site
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
codon start: 1
label: TEV site
note: TEV site
product: tobacco etch virus (TEV) protease recognition and cleavage site
translation: MENLYFQ
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA064/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA064/annotation7/range/1

https://synbiohub.org/public/SEGA/SEGA064/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA064/1
start: 1868
end: 1888
orientation: inline

lacI
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
codon start: 1
gene: lacI
label: lacI
note: The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG).
note: lacI
product: lac repressor
translation: MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA064/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA064/annotation2/range/1

https://synbiohub.org/public/SEGA/SEGA064/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA064/1
start: 604
end: 1686
orientation: reverseComplement

lacI promoter
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
gene: lacI
label: lacI promoter
note: lacI promoter
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA064/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA064/annotation3/range/1

https://synbiohub.org/public/SEGA/SEGA064/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA064/1
start: 1687
end: 1764
orientation: reverseComplement

glmS
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
codon start: 1
label: glmS
note: glmS
translation: VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA064/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA064/annotation0/range/1

https://synbiohub.org/public/SEGA/SEGA064/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA064/1
start: 1
end: 528
orientation: inline

Promoter 3BCD2
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
label: Promoter 3BCD2
note: Promoter 3BCD2
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA064/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA064/annotation10/range/1

https://synbiohub.org/public/SEGA/SEGA064/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA064/1
start: 2662
end: 2690
orientation: inline

lac operator
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
bound moiety: lac repressor encoded by lacI
feature type: protein_bind
label: lac operator
note: The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG).
note: lac operator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA064/1
role: SO:0000110
location: https://synbiohub.org/public/SEGA/SEGA064/annotation5/range/1

https://synbiohub.org/public/SEGA/SEGA064/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA064/1
start: 1814
end: 1838
orientation: inline

high TIR
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
note: TIR
note: high TIR
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA064/1
role: SO:0000139
location: https://synbiohub.org/public/SEGA/SEGA064/annotation6/range/1

https://synbiohub.org/public/SEGA/SEGA064/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA064/1
start: 1850
end: 1876
orientation: inline

rrnC
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
label: rrnC
note: rrnC
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA064/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA064/annotation12/range/1

https://synbiohub.org/public/SEGA/SEGA064/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA064/1
start: 3931
end: 3971
orientation: inline

TcR
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
codon start: 1
gene: tet
label: TcR
note: TcR
note: confers resistance to tetracycline
product: tetracycline efflux protein
translation: MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHYGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVIFGEDRFRWSATMSGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA064/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA064/annotation11/range/1

https://synbiohub.org/public/SEGA/SEGA064/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA064/1
start: 2730
end: 3920
orientation: inline

https://synbiohub.org/public/SEGA/SEGA029/1
type: ComponentDefinition
persistent identity: SEGA029
display id: SEGA029
version: 1
description: natural linear DNA.
owned by: carbay
owned by: morno
date: 08-SEP-2021
division: UNA
keywords: .
locus: SEGA029
molecule: ds-DNA
organism: unspecified
reference: https://synbiohub.org/user/carbay/SEGA/SEGA029_reference0/1
source: natural DNA sequence
type: DnaRegion
type: SO:0000987
role: SO:0000804
sequence annotation: The trc promoter combines the -35 region from the trp promoter and the -10 region from the lacUV5 promoter
sequence annotation: lacI promoter
sequence annotation: lacO
sequence annotation: pstS
sequence annotation: rrnC
sequence annotation: Promoter 3BCD2
sequence annotation: GFP
sequence annotation: L3S3P22 Terminator (synthetic)
sequence annotation: L3S2P11 Terminator
sequence annotation: low TIR
sequence annotation: lacI
sequence annotation: TEV site
sequence annotation: glmS
sequence annotation: TcR
sequence annotation: PROTi-Linker
sequence: https://synbiohub.org/public/SEGA/SEGA029_seq/1

The trc promoter combines the -35 region from the trp promoter and the -10 region from the lacUV5 promoter
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
note: Ptrc
note: The trc promoter combines the -35 region from the trp promoter and the -10 region from the lacUV5 promoter
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA029/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA029/annotation4/range/1

https://synbiohub.org/public/SEGA/SEGA029/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA029/1
start: 1795
end: 1824
orientation: inline

lacI promoter
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
gene: lacI
label: lacI promoter
note: lacI promoter
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA029/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA029/annotation3/range/1

https://synbiohub.org/public/SEGA/SEGA029/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA029/1
start: 1687
end: 1764
orientation: reverseComplement

lacO
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
allele: lac operator
bound moiety: lac repressor
feature type: protein_bind
label: lacO
note: lacO
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA029/1
role: SO:0000110
location: https://synbiohub.org/public/SEGA/SEGA029/annotation5/range/1

https://synbiohub.org/public/SEGA/SEGA029/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA029/1
start: 1825
end: 1848
orientation: inline

pstS
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
codon start: 1
label: pstS
note: pstS
translation: MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKWADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA029/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA029/annotation14/range/1

https://synbiohub.org/public/SEGA/SEGA029/annotation14/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA029/1
start: 4212
end: 4459
orientation: inline

rrnC
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
label: rrnC
note: rrnC
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA029/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA029/annotation13/range/1

https://synbiohub.org/public/SEGA/SEGA029/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA029/1
start: 3977
end: 4017
orientation: inline

Promoter 3BCD2
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
label: Promoter 3BCD2
note: Promoter 3BCD2
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA029/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA029/annotation11/range/1

https://synbiohub.org/public/SEGA/SEGA029/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA029/1
start: 2708
end: 2736
orientation: inline

GFP
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
codon start: 1
label: GFP
note: GFP
product: green fluoresent protein
translation: SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYK
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA029/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA029/annotation9/range/1

https://synbiohub.org/public/SEGA/SEGA029/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA029/1
start: 1935
end: 2645
orientation: inline

L3S3P22 Terminator (synthetic)
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
label: L3S3P22 Terminator (synthetic)
note: L3S3P22 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA029/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA029/annotation10/range/1

https://synbiohub.org/public/SEGA/SEGA029/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA029/1
start: 2649
end: 2697
orientation: inline

L3S2P11 Terminator
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
label: L3S2P11 Terminator
note: L3S2P11 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA029/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA029/annotation1/range/1

https://synbiohub.org/public/SEGA/SEGA029/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA029/1
start: 538
end: 594
orientation: inline

low TIR
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
note: TIR
note: low TIR
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA029/1
role: SO:0000139
location: https://synbiohub.org/public/SEGA/SEGA029/annotation6/range/1

https://synbiohub.org/public/SEGA/SEGA029/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA029/1
start: 1860
end: 1886
orientation: inline

lacI
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
codon start: 1
gene: lacI
label: lacI
note: The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG).
note: lacI
product: lac repressor
translation: MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA029/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA029/annotation2/range/1

https://synbiohub.org/public/SEGA/SEGA029/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA029/1
start: 604
end: 1686
orientation: reverseComplement

TEV site
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
codon start: 1
label: TEV site
note: TEV site
product: tobacco etch virus (TEV) protease recognition and cleavage site
translation: MENLYFQ
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA029/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA029/annotation7/range/1

https://synbiohub.org/public/SEGA/SEGA029/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA029/1
start: 1878
end: 1898
orientation: inline

glmS
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
codon start: 1
label: glmS
note: glmS
translation: VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA029/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA029/annotation0/range/1

https://synbiohub.org/public/SEGA/SEGA029/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA029/1
start: 1
end: 528
orientation: inline

TcR
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
codon start: 1
gene: tet
label: TcR
note: TcR
note: confers resistance to tetracycline
product: tetracycline efflux protein
translation: MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHYGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVIFGEDRFRWSATMSGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA029/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA029/annotation12/range/1

https://synbiohub.org/public/SEGA/SEGA029/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA029/1
start: 2776
end: 3966
orientation: inline

PROTi-Linker
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
label: PROTi-Linker
note: PROTi-Linker
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA029/1
role: SO:0001411
location: https://synbiohub.org/public/SEGA/SEGA029/annotation8/range/1

https://synbiohub.org/public/SEGA/SEGA029/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA029/1
start: 1899
end: 1934
orientation: inline

https://synbiohub.org/public/SEGA/SEGA015/1
type: ComponentDefinition
persistent identity: SEGA015
display id: SEGA015
version: 1
description: natural linear DNA.
owned by: carbay
owned by: morno
date: 09-SEP-2021
division: UNA
keywords: .
locus: SEGA015
molecule: ds-DNA
organism: unspecified
reference: https://synbiohub.org/user/carbay/SEGA/SEGA015_reference0/1
source: natural DNA sequence
type: DnaRegion
type: SO:0000987
role: SO:0000804
sequence annotation: rrnC
sequence annotation: TcR
sequence annotation: Promoter 3BCD2
sequence annotation: P-J23100
sequence annotation: L3S2P11 Terminator
sequence annotation: pstS
sequence annotation: L3S3P22 Terminator (synthetic)
sequence annotation: PROTi-Linker
sequence annotation: glmS
sequence annotation: TEV site
sequence annotation: GFP
sequence annotation: medium TIR
sequence: https://synbiohub.org/public/SEGA/SEGA015_seq/1

rrnC
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
label: rrnC
note: rrnC
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA015/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA015/annotation10/range/1

https://synbiohub.org/public/SEGA/SEGA015/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA015/1
start: 2767
end: 2807
orientation: inline

TcR
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
codon start: 1
gene: tet
label: TcR
note: TcR
note: confers resistance to tetracycline
product: tetracycline efflux protein
translation: MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHYGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVIFGEDRFRWSATMSGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA015/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA015/annotation9/range/1

https://synbiohub.org/public/SEGA/SEGA015/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA015/1
start: 1566
end: 2756
orientation: inline

Promoter 3BCD2
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
label: Promoter 3BCD2
note: Promoter 3BCD2
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA015/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA015/annotation8/range/1

https://synbiohub.org/public/SEGA/SEGA015/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA015/1
start: 1498
end: 1526
orientation: inline

P-J23100
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
label: P-J23100
note: P-J23100
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA015/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA015/annotation2/range/1

https://synbiohub.org/public/SEGA/SEGA015/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA015/1
start: 604
end: 638
orientation: inline

L3S2P11 Terminator
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
label: L3S2P11 Terminator
note: L3S2P11 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA015/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA015/annotation1/range/1

https://synbiohub.org/public/SEGA/SEGA015/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA015/1
start: 538
end: 594
orientation: inline

pstS
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
codon start: 1
label: pstS
note: pstS
translation: MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKWADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA015/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA015/annotation11/range/1

https://synbiohub.org/public/SEGA/SEGA015/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA015/1
start: 3002
end: 3249
orientation: inline

L3S3P22 Terminator (synthetic)
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
label: L3S3P22 Terminator (synthetic)
note: L3S3P22 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA015/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA015/annotation7/range/1

https://synbiohub.org/public/SEGA/SEGA015/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA015/1
start: 1439
end: 1487
orientation: inline

PROTi-Linker
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
label: PROTi-Linker
note: PROTi-Linker
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA015/1
role: SO:0001411
location: https://synbiohub.org/public/SEGA/SEGA015/annotation5/range/1

https://synbiohub.org/public/SEGA/SEGA015/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA015/1
start: 689
end: 724
orientation: inline

glmS
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
codon start: 1
label: glmS
note: glmS
translation: VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA015/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA015/annotation0/range/1

https://synbiohub.org/public/SEGA/SEGA015/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA015/1
start: 1
end: 528
orientation: inline

TEV site
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
codon start: 1
label: TEV site
note: TEV site
product: tobacco etch virus (TEV) protease recognition and cleavage site
translation: MENLYFQ
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA015/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA015/annotation4/range/1

https://synbiohub.org/public/SEGA/SEGA015/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA015/1
start: 668
end: 688
orientation: inline

GFP
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
codon start: 1
label: GFP
note: GFP
product: green fluorescent protein
translation: SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYK
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA015/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA015/annotation6/range/1

https://synbiohub.org/public/SEGA/SEGA015/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA015/1
start: 725
end: 1435
orientation: inline

medium TIR
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
note: TIR
note: medium TIR
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA015/1
role: SO:0000139
location: https://synbiohub.org/public/SEGA/SEGA015/annotation3/range/1

https://synbiohub.org/public/SEGA/SEGA015/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA015/1
start: 650
end: 676
orientation: inline

https://synbiohub.org/public/SEGA/SEGA017/1
type: ComponentDefinition
persistent identity: SEGA017
display id: SEGA017
version: 1
description: natural linear DNA.
owned by: carbay
owned by: morno
date: 09-SEP-2021
division: UNA
keywords: .
locus: SEGA017
molecule: ds-DNA
organism: unspecified
reference: https://synbiohub.org/public/SEGA/reference0_1/1
source: natural DNA sequence
type: DnaRegion
type: SO:0000987
role: SO:0000804
sequence annotation: P-J23100
sequence annotation: L3S2P11 Terminator
sequence annotation: L3S3P22 Terminator (synthetic)
sequence annotation: rrnC
sequence annotation: low TIR
sequence annotation: glmS
sequence annotation: TcR
sequence annotation: Promoter 3BCD2
sequence annotation: sfGFP
sequence annotation: pelB signal sequence
sequence annotation: pstS
sequence: https://synbiohub.org/public/SEGA/SEGA017_seq/1

P-J23100
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
label: P-J23100
note: P-J23100
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA017/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA017/annotation2/range/1

https://synbiohub.org/public/SEGA/SEGA017/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA017/1
start: 604
end: 638
orientation: inline

L3S2P11 Terminator
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
label: L3S2P11 Terminator
note: L3S2P11 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA017/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA017/annotation1/range/1

https://synbiohub.org/public/SEGA/SEGA017/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA017/1
start: 538
end: 594
orientation: inline

L3S3P22 Terminator (synthetic)
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
label: L3S3P22 Terminator (synthetic)
note: L3S3P22 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA017/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA017/annotation6/range/1

https://synbiohub.org/public/SEGA/SEGA017/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA017/1
start: 1448
end: 1496
orientation: inline

rrnC
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
label: rrnC
note: rrnC
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA017/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA017/annotation9/range/1

https://synbiohub.org/public/SEGA/SEGA017/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA017/1
start: 2776
end: 2816
orientation: inline

low TIR
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
note: TIR
note: low TIR
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA017/1
role: SO:0000139
location: https://synbiohub.org/public/SEGA/SEGA017/annotation3/range/1

https://synbiohub.org/public/SEGA/SEGA017/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA017/1
start: 650
end: 676
orientation: inline

glmS
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
codon start: 1
label: glmS
note: glmS
translation: VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA017/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA017/annotation0/range/1

https://synbiohub.org/public/SEGA/SEGA017/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA017/1
start: 1
end: 528
orientation: inline

TcR
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
codon start: 1
gene: tet
label: TcR
note: TcR
note: confers resistance to tetracycline
product: tetracycline efflux protein
translation: MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHYGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVIFGEDRFRWSATMSGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA017/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA017/annotation8/range/1

https://synbiohub.org/public/SEGA/SEGA017/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA017/1
start: 1575
end: 2765
orientation: inline

Promoter 3BCD2
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
label: Promoter 3BCD2
note: Promoter 3BCD2
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA017/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA017/annotation7/range/1

https://synbiohub.org/public/SEGA/SEGA017/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA017/1
start: 1507
end: 1535
orientation: inline

sfGFP
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
codon start: 1
label: sfGFP
note: sfGFP
product: superfolder GFP
translation: RKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDGTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEFVTAAGITHGMDELYK
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA017/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA017/annotation5/range/1

https://synbiohub.org/public/SEGA/SEGA017/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA017/1
start: 734
end: 1444
orientation: inline

pelB signal sequence
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
codon start: 1
gene: pelB (fragment)
label: pelB signal sequence
note: pelB signal sequence
product: leader peptide for secretion
translation: MKYLLPTAAAGLLLLAAQPAMA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA017/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA017/annotation4/range/1

https://synbiohub.org/public/SEGA/SEGA017/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA017/1
start: 668
end: 733
orientation: inline

pstS
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
codon start: 1
label: pstS
note: pstS
translation: MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKWADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA017/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA017/annotation10/range/1

https://synbiohub.org/public/SEGA/SEGA017/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA017/1
start: 3011
end: 3258
orientation: inline

https://synbiohub.org/public/SEGA/SEGA079/1
type: ComponentDefinition
persistent identity: SEGA079
display id: SEGA079
version: 1
description: natural linear DNA.
owned by: carbay
owned by: morno
date: 09-SEP-2021
division: UNA
keywords: .
locus: SEGA079
molecule: ds-DNA
organism: unspecified
reference: https://synbiohub.org/user/carbay/SEGA/SEGA079_reference0/1
source: natural DNA sequence
type: DnaRegion
type: SO:0000987
role: SO:0000804
sequence annotation: L3S2P11 Terminator
sequence annotation: rhaB promoter
sequence annotation: Promoter 3BCD2
sequence annotation: Terminator rrnB T1
sequence annotation: rrnC
sequence annotation: L3S3P22
sequence annotation: pstS
sequence annotation: rhaR
sequence annotation: GFP
sequence annotation: TcR
sequence annotation: BCD
sequence annotation: medium TIR
sequence annotation: glmS
sequence annotation: EM7 promoter
sequence annotation: rhaS
sequence annotation: galK
sequence: https://synbiohub.org/public/SEGA/SEGA079_seq/1

L3S2P11 Terminator
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
label: L3S2P11 Terminator
note: L3S2P11 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA079/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA079/annotation1/range/1

https://synbiohub.org/public/SEGA/SEGA079/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA079/1
start: 538
end: 594
orientation: inline

rhaB promoter
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
label: rhaB promoter
note: promoter of the E. coli rhaBAD operon, conferring tight induction with L-rhamnose and repression with D-glucose in the presence of RhaR and RhaS (Giacalone et al., 2006)
note: rhaB promoter
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA079/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA079/annotation7/range/1

https://synbiohub.org/public/SEGA/SEGA079/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA079/1
start: 3800
end: 3918
orientation: inline

Promoter 3BCD2
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
label: Promoter 3BCD2
note: Promoter 3BCD2
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA079/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA079/annotation12/range/1

https://synbiohub.org/public/SEGA/SEGA079/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA079/1
start: 4777
end: 4805
orientation: inline

Terminator rrnB T1
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
label: Terminator rrnB T1
label: rrnB
note: rrnB
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA079/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA079/annotation4/range/1

https://synbiohub.org/public/SEGA/SEGA079/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA079/1
start: 1843
end: 1886
orientation: inline

rrnC
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
label: rrnC
note: rrnC
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA079/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA079/annotation14/range/1

https://synbiohub.org/public/SEGA/SEGA079/annotation14/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA079/1
start: 6046
end: 6086
orientation: inline

L3S3P22
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
label: L3S3P22
note: L3S3P22
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA079/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA079/annotation11/range/1

https://synbiohub.org/public/SEGA/SEGA079/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA079/1
start: 4718
end: 4766
orientation: inline

pstS
type: SequenceAnnotation
persistent identity: annotation15
display id: annotation15
version: 1
codon start: 1
label: pstS
note: pstS
translation: MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKWADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA079/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA079/annotation15/range/1

https://synbiohub.org/public/SEGA/SEGA079/annotation15/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA079/1
start: 6281
end: 6528
orientation: inline

rhaR
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
codon start: 1
gene: E. coli rhaR
label: rhaR
note: needed for rhamnose-dependent activation of the rhaB promoter
note: rhaR
product: transcriptional activator of rhaR and rhaS
translation: MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQGAIPGFNASAGQPHWRLGSMGMAQARQVIGQLEHESSQHVPFANEMAELLFGQLVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRHLNSQKD
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA079/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA079/annotation5/range/1

https://synbiohub.org/public/SEGA/SEGA079/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA079/1
start: 1899
end: 2744
orientation: reverseComplement

GFP
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
codon start: 1
label: GFP
note: GFP
product: green fluorescent protein
translation: SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYK
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA079/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA079/annotation10/range/1

https://synbiohub.org/public/SEGA/SEGA079/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA079/1
start: 4004
end: 4714
orientation: inline

TcR
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
codon start: 1
gene: tet
label: TcR
note: TcR
note: confers resistance to tetracycline
product: tetracycline efflux protein
translation: MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHYGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVIFGEDRFRWSATMSGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA079/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA079/annotation13/range/1

https://synbiohub.org/public/SEGA/SEGA079/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA079/1
start: 4845
end: 6035
orientation: inline

BCD
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
codon start: 1
label: BCD
note: BCD
translation: MKAIFVLKHLNHAKEVF
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA079/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA079/annotation9/range/1

https://synbiohub.org/public/SEGA/SEGA079/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA079/1
start: 3948
end: 4003
orientation: inline

medium TIR
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
note: TIR
note: medium TIR
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA079/1
role: SO:0000139
location: https://synbiohub.org/public/SEGA/SEGA079/annotation8/range/1

https://synbiohub.org/public/SEGA/SEGA079/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA079/1
start: 3930
end: 3956
orientation: inline

glmS
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
codon start: 1
label: glmS
note: glmS
translation: VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA079/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA079/annotation0/range/1

https://synbiohub.org/public/SEGA/SEGA079/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA079/1
start: 1
end: 528
orientation: inline

EM7 promoter
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
label: EM7 promoter
note: EM7 promoter
note: synthetic bacterial promoter
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA079/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA079/annotation3/range/1

https://synbiohub.org/public/SEGA/SEGA079/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA079/1
start: 1783
end: 1830
orientation: reverseComplement

rhaS
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
codon start: 1
gene: E. coli rhaS
label: rhaS
note: activated by L-rhamnose
note: rhaS
product: positive regulator of the rhaB promoter
translation: MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHGTGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLLPQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKSSLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQRYLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGFLQ
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA079/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA079/annotation6/range/1

https://synbiohub.org/public/SEGA/SEGA079/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA079/1
start: 2818
end: 3654
orientation: reverseComplement

galK
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
EC number: 2.7.1.6
codon start: 1
db xref: ASAP:ABE-0002568
db xref: EcoGene:EG10363
db xref: GI:16128725
db xref: GeneID:945358
db xref: UniProtKB/Swiss-Prot:P0A6T3
function: enzyme; Degradation of small molecules: Carbon compounds
gene: galK
gene synonym: ECK0746; galA; JW0740
label: galK
locus tag: b0757
note: galK
product: galactokinase
protein id: NP_415278.1
translation: MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVLPCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLDAPIVAHENYQWANYVRGVVKHLQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQEAENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTLVGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTVEAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGGGFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETLYVCKPSQGAGQC
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA079/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA079/annotation2/range/1

https://synbiohub.org/public/SEGA/SEGA079/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA079/1
start: 605
end: 1753
orientation: reverseComplement

https://synbiohub.org/public/SEGA/SEGA031/1
type: ComponentDefinition
persistent identity: SEGA031
display id: SEGA031
version: 1
description: natural linear DNA.
owned by: carbay
owned by: morno
date: 08-SEP-2021
division: UNA
keywords: .
locus: SEGA031
molecule: ds-DNA
organism: unspecified
reference: https://synbiohub.org/public/SEGA/reference0_1/1
source: natural DNA sequence
type: DnaRegion
type: SO:0000987
role: SO:0000804
sequence annotation: lacI
sequence annotation: pstS
sequence annotation: high TIR
sequence annotation: L3S3P22 Terminator (synthetic)
sequence annotation: TEV site
sequence annotation: GFP
sequence annotation: Promoter 3BCD2
sequence annotation: trc promoter
sequence annotation: lacO
sequence annotation: glmS
sequence annotation: L3S2P11 Terminator
sequence annotation: lacI promoter
sequence annotation: PROTi-Linker
sequence annotation: TcR
sequence annotation: rrnC
sequence: https://synbiohub.org/public/SEGA/SEGA031_seq/1

lacI
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
codon start: 1
gene: lacI
label: lacI
note: The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG).
note: lacI
product: lac repressor
translation: MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA031/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA031/annotation2/range/1

https://synbiohub.org/public/SEGA/SEGA031/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA031/1
start: 604
end: 1686
orientation: reverseComplement

pstS
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
codon start: 1
label: pstS
note: pstS
translation: MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKWADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA031/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA031/annotation14/range/1

https://synbiohub.org/public/SEGA/SEGA031/annotation14/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA031/1
start: 4212
end: 4459
orientation: inline

high TIR
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
note: TIR
note: high TIR
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA031/1
role: SO:0000139
location: https://synbiohub.org/public/SEGA/SEGA031/annotation6/range/1

https://synbiohub.org/public/SEGA/SEGA031/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA031/1
start: 1860
end: 1886
orientation: inline

L3S3P22 Terminator (synthetic)
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
label: L3S3P22 Terminator (synthetic)
note: L3S3P22 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA031/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA031/annotation10/range/1

https://synbiohub.org/public/SEGA/SEGA031/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA031/1
start: 2649
end: 2697
orientation: inline

TEV site
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
codon start: 1
label: TEV site
note: TEV site
product: tobacco etch virus (TEV) protease recognition and cleavage site
translation: MENLYFQ
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA031/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA031/annotation7/range/1

https://synbiohub.org/public/SEGA/SEGA031/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA031/1
start: 1878
end: 1898
orientation: inline

GFP
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
codon start: 1
label: GFP
note: GFP
product: green fluorescent protein
translation: SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYK
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA031/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA031/annotation9/range/1

https://synbiohub.org/public/SEGA/SEGA031/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA031/1
start: 1935
end: 2645
orientation: inline

Promoter 3BCD2
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
label: Promoter 3BCD2
note: Promoter 3BCD2
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA031/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA031/annotation11/range/1

https://synbiohub.org/public/SEGA/SEGA031/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA031/1
start: 2708
end: 2736
orientation: inline

trc promoter
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
label: trc promoter
note: strong E. coli promoter; hybrid between the trp and lac UV5 promoters
note: trc promoter
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA031/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA031/annotation4/range/1

https://synbiohub.org/public/SEGA/SEGA031/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA031/1
start: 1795
end: 1824
orientation: inline

lacO
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
bound moiety: lac repressor
feature type: protein_bind
label: lacO
note: lac operator
note: lacO
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA031/1
role: SO:0000110
location: https://synbiohub.org/public/SEGA/SEGA031/annotation5/range/1

https://synbiohub.org/public/SEGA/SEGA031/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA031/1
start: 1825
end: 1848
orientation: inline

glmS
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
codon start: 1
label: glmS
note: glmS
translation: VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA031/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA031/annotation0/range/1

https://synbiohub.org/public/SEGA/SEGA031/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA031/1
start: 1
end: 528
orientation: inline

L3S2P11 Terminator
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
label: L3S2P11 Terminator
note: L3S2P11 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA031/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA031/annotation1/range/1

https://synbiohub.org/public/SEGA/SEGA031/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA031/1
start: 538
end: 594
orientation: inline

lacI promoter
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
gene: lacI
label: lacI promoter
note: lacI promoter
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA031/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA031/annotation3/range/1

https://synbiohub.org/public/SEGA/SEGA031/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA031/1
start: 1687
end: 1764
orientation: reverseComplement

PROTi-Linker
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
label: PROTi-Linker
note: PROTi-Linker
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA031/1
role: SO:0001411
location: https://synbiohub.org/public/SEGA/SEGA031/annotation8/range/1

https://synbiohub.org/public/SEGA/SEGA031/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA031/1
start: 1899
end: 1934
orientation: inline

TcR
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
codon start: 1
gene: tet
label: TcR
note: TcR
note: confers resistance to tetracycline
product: tetracycline efflux protein
translation: MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHYGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVIFGEDRFRWSATMSGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA031/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA031/annotation12/range/1

https://synbiohub.org/public/SEGA/SEGA031/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA031/1
start: 2776
end: 3966
orientation: inline

rrnC
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
label: rrnC
note: rrnC
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA031/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA031/annotation13/range/1

https://synbiohub.org/public/SEGA/SEGA031/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA031/1
start: 3977
end: 4017
orientation: inline

https://synbiohub.org/public/SEGA/SEGA050/1
type: ComponentDefinition
persistent identity: SEGA050
display id: SEGA050
version: 1
description: natural linear DNA.
owned by: carbay
owned by: morno
date: 08-SEP-2021
division: UNA
keywords: .
locus: SEGA050
molecule: ds-DNA
organism: unspecified
reference: https://synbiohub.org/user/carbay/SEGA/SEGA050_reference0/1
source: natural DNA sequence
type: DnaRegion
type: SO:0000987
role: SO:0000804
sequence annotation: pstS
sequence annotation: glmS
sequence annotation: rhaS
sequence annotation: L3S2P11 Terminator
sequence annotation: pelB signal sequence
sequence annotation: TcR
sequence annotation: Promoter 3BCD2
sequence annotation: L3S3P22 Terminator (synthetic)
sequence annotation: rrnC
sequence annotation: rhaR
sequence annotation: low TIR
sequence annotation: rhaB promoter
sequence annotation: sfGFP
sequence: https://synbiohub.org/public/SEGA/SEGA050_seq/1

pstS
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
codon start: 1
label: pstS
note: pstS
translation: MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKWADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA050/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA050/annotation12/range/1

https://synbiohub.org/public/SEGA/SEGA050/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA050/1
start: 4999
end: 5246
orientation: inline

glmS
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
codon start: 1
label: glmS
note: glmS
translation: VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA050/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA050/annotation0/range/1

https://synbiohub.org/public/SEGA/SEGA050/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA050/1
start: 1
end: 528
orientation: inline

rhaS
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
codon start: 1
gene: E. coli rhaS
label: rhaS
note: activated by L-rhamnose
note: rhaS
product: positive regulator of the rhaB promoter
translation: MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHGTGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLLPQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKSSLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQRYLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGFLQ
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA050/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA050/annotation3/range/1

https://synbiohub.org/public/SEGA/SEGA050/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA050/1
start: 1526
end: 2362
orientation: reverseComplement

L3S2P11 Terminator
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
label: L3S2P11 Terminator
note: L3S2P11 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA050/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA050/annotation1/range/1

https://synbiohub.org/public/SEGA/SEGA050/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA050/1
start: 538
end: 594
orientation: inline

pelB signal sequence
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
codon start: 1
gene: pelB (fragment)
label: pelB signal sequence
note: pelB signal sequence
product: leader peptide for secretion
translation: MKYLLPTAAAGLLLLAAQPAMA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA050/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA050/annotation6/range/1

https://synbiohub.org/public/SEGA/SEGA050/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA050/1
start: 2656
end: 2721
orientation: inline

TcR
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
codon start: 1
gene: tet
label: TcR
note: TcR
note: confers resistance to tetracycline
product: tetracycline efflux protein
translation: MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHYGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVIFGEDRFRWSATMSGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA050/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA050/annotation10/range/1

https://synbiohub.org/public/SEGA/SEGA050/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA050/1
start: 3563
end: 4753
orientation: inline

Promoter 3BCD2
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
label: Promoter 3BCD2
note: Promoter 3BCD2
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA050/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA050/annotation9/range/1

https://synbiohub.org/public/SEGA/SEGA050/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA050/1
start: 3495
end: 3523
orientation: inline

L3S3P22 Terminator (synthetic)
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
label: L3S3P22 Terminator (synthetic)
note: L3S3P22 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA050/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA050/annotation8/range/1

https://synbiohub.org/public/SEGA/SEGA050/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA050/1
start: 3436
end: 3484
orientation: inline

rrnC
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
label: rrnC
note: rrnC
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA050/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA050/annotation11/range/1

https://synbiohub.org/public/SEGA/SEGA050/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA050/1
start: 4764
end: 4804
orientation: inline

rhaR
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
codon start: 1
gene: E. coli rhaR
label: rhaR
note: needed for rhamnose-dependent activation of the rhaB promoter
note: rhaR
product: transcriptional activator of rhaR and rhaS
translation: MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQGAIPGFNASAGQPHWRLGSMGMAQARQVIGQLEHESSQHVPFANEMAELLFGQLVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRHLNSQKD
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA050/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA050/annotation2/range/1

https://synbiohub.org/public/SEGA/SEGA050/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA050/1
start: 607
end: 1452
orientation: reverseComplement

low TIR
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
note: TIR
note: low TIR
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA050/1
role: SO:0000139
location: https://synbiohub.org/public/SEGA/SEGA050/annotation5/range/1

https://synbiohub.org/public/SEGA/SEGA050/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA050/1
start: 2638
end: 2664
orientation: inline

rhaB promoter
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
label: rhaB promoter
note: promoter of the E. coli rhaBAD operon, conferring tight induction with L-rhamnose and repression with D-glucose in the presence of RhaR and RhaS (Giacalone et al., 2006)
note: rhaB promoter
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA050/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA050/annotation4/range/1

https://synbiohub.org/public/SEGA/SEGA050/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA050/1
start: 2508
end: 2626
orientation: inline

sfGFP
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
codon start: 1
label: sfGFP
note: sfGFP
product: superfolder GFP
translation: RKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDGTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEFVTAAGITHGMDELYK
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA050/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA050/annotation7/range/1

https://synbiohub.org/public/SEGA/SEGA050/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA050/1
start: 2722
end: 3432
orientation: inline

https://synbiohub.org/public/SEGA/SEGA003/1
type: ComponentDefinition
persistent identity: SEGA003
display id: SEGA003
version: 1
description: natural linear DNA.
owned by: carbay
owned by: morno
date: 08-SEP-2021
division: UNA
keywords: .
locus: SEGA003
molecule: ds-DNA
organism: unspecified
reference: https://synbiohub.org/user/carbay/SEGA/SEGA003_reference0/1
source: natural DNA sequence
type: DnaRegion
type: SO:0000987
role: SO:0000804
sequence annotation: RBS
sequence annotation: glmS
sequence annotation: rrnC
sequence annotation: pstS
sequence annotation: TcR
sequence annotation: rhaR
sequence annotation: L3S2P11 Terminator
sequence annotation: rhaB promoter
sequence annotation: rhaS
sequence: https://synbiohub.org/public/SEGA/SEGA003_seq/1

RBS
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
note: RBS
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA003/1
role: SO:0000139
location: https://synbiohub.org/public/SEGA/SEGA003/annotation5/range/1

https://synbiohub.org/public/SEGA/SEGA003/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA003/1
start: 2638
end: 2649
orientation: inline

glmS
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
codon start: 1
label: glmS
note: glmS
translation: VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA003/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA003/annotation0/range/1

https://synbiohub.org/public/SEGA/SEGA003/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA003/1
start: 1
end: 528
orientation: inline

rrnC
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
label: rrnC
note: rrnC
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA003/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA003/annotation7/range/1

https://synbiohub.org/public/SEGA/SEGA003/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA003/1
start: 3773
end: 3813
orientation: inline

pstS
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
codon start: 1
label: pstS
note: pstS
translation: MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKWADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA003/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA003/annotation8/range/1

https://synbiohub.org/public/SEGA/SEGA003/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA003/1
start: 4008
end: 4255
orientation: inline

TcR
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
codon start: 1
gene: tet
label: TcR
note: TcR
note: confers resistance to tetracycline
product: tetracycline efflux protein
translation: VLPGLLRDIVHSDSIASHYGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVIFGEDRFRWSATMSGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA003/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA003/annotation6/range/1

https://synbiohub.org/public/SEGA/SEGA003/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA003/1
start: 2650
end: 3762
orientation: inline

rhaR
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
codon start: 1
gene: E. coli rhaR
label: rhaR
note: needed for rhamnose-dependent activation of the rhaB promoter
note: rhaR
product: transcriptional activator of rhaR and rhaS
translation: MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQGAIPGFNASAGQPHWRLGSMGMAQARQVIGQLEHESSQHVPFANEMAELLFGQLVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRHLNSQKD
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA003/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA003/annotation2/range/1

https://synbiohub.org/public/SEGA/SEGA003/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA003/1
start: 607
end: 1452
orientation: reverseComplement

L3S2P11 Terminator
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
label: L3S2P11 Terminator
note: L3S2P11 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA003/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA003/annotation1/range/1

https://synbiohub.org/public/SEGA/SEGA003/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA003/1
start: 538
end: 594
orientation: inline

rhaB promoter
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
label: rhaB promoter
note: promoter of the E. coli rhaBAD operon, conferring tight induction with L-rhamnose and repression with D-glucose in the presence of RhaR and RhaS
note: rhaB promoter
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA003/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA003/annotation4/range/1

https://synbiohub.org/public/SEGA/SEGA003/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA003/1
start: 2508
end: 2626
orientation: inline

rhaS
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
codon start: 1
gene: E. coli rhaS
label: rhaS
note: activated by L-rhamnose
note: rhaS
product: positive regulator of the rhaB promoter
translation: MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHGTGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLLPQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKSSLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQRYLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGFLQ
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA003/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA003/annotation3/range/1

https://synbiohub.org/public/SEGA/SEGA003/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA003/1
start: 1526
end: 2362
orientation: reverseComplement

https://synbiohub.org/public/SEGA/SEGA039/1
type: ComponentDefinition
persistent identity: SEGA039
display id: SEGA039
version: 1
description: natural linear DNA.
owned by: carbay
owned by: morno
date: 08-SEP-2021
division: UNA
keywords: .
locus: SEGA039
molecule: ds-DNA
organism: unspecified
reference: https://synbiohub.org/user/carbay/SEGA/SEGA039_reference0/1
source: natural DNA sequence
type: DnaRegion
type: SO:0000987
role: SO:0000804
sequence annotation: rhaS
sequence annotation: TcR
sequence annotation: L3S3P22 Terminator (synthetic)
sequence annotation: rrnC
sequence annotation: rhaR
sequence annotation: glmS
sequence annotation: GFP
sequence annotation: L3S2P11 Terminator
sequence annotation: pstS
sequence annotation: BCD
sequence annotation: Promoter 3BCD2
sequence annotation: rhaB promoter
sequence annotation: medium TIR
sequence: https://synbiohub.org/public/SEGA/SEGA039_seq/1

rhaS
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
codon start: 1
gene: E. coli rhaS
label: rhaS
note: activated by L-rhamnose
note: rhaS
product: positive regulator of the rhaB promoter
translation: MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHGTGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLLPQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKSSLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQRYLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGFLQ
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA039/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA039/annotation3/range/1

https://synbiohub.org/public/SEGA/SEGA039/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA039/1
start: 1526
end: 2362
orientation: reverseComplement

TcR
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
codon start: 1
gene: tet
label: TcR
note: TcR
note: confers resistance to tetracycline
product: tetracycline efflux protein
translation: MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHYGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVIFGEDRFRWSATMSGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA039/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA039/annotation10/range/1

https://synbiohub.org/public/SEGA/SEGA039/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA039/1
start: 3553
end: 4743
orientation: inline

L3S3P22 Terminator (synthetic)
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
label: L3S3P22 Terminator (synthetic)
note: L3S3P22 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA039/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA039/annotation8/range/1

https://synbiohub.org/public/SEGA/SEGA039/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA039/1
start: 3426
end: 3474
orientation: inline

rrnC
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
label: rrnC
note: rrnC
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA039/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA039/annotation11/range/1

https://synbiohub.org/public/SEGA/SEGA039/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA039/1
start: 4754
end: 4794
orientation: inline

rhaR
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
codon start: 1
gene: E. coli rhaR
label: rhaR
note: needed for rhamnose-dependent activation of the rhaB promoter
note: rhaR
product: transcriptional activator of rhaR and rhaS
translation: MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQGAIPGFNASAGQPHWRLGSMGMAQARQVIGQLEHESSQHVPFANEMAELLFGQLVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRHLNSQKD
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA039/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA039/annotation2/range/1

https://synbiohub.org/public/SEGA/SEGA039/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA039/1
start: 607
end: 1452
orientation: reverseComplement

glmS
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
codon start: 1
label: glmS
note: glmS
translation: VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA039/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA039/annotation0/range/1

https://synbiohub.org/public/SEGA/SEGA039/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA039/1
start: 1
end: 528
orientation: inline

GFP
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
codon start: 1
label: GFP
note: GFP
product: green fluorescent protein
translation: SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYK
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA039/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA039/annotation7/range/1

https://synbiohub.org/public/SEGA/SEGA039/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA039/1
start: 2712
end: 3422
orientation: inline

L3S2P11 Terminator
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
label: L3S2P11 Terminator
note: L3S2P11 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA039/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA039/annotation1/range/1

https://synbiohub.org/public/SEGA/SEGA039/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA039/1
start: 538
end: 594
orientation: inline

pstS
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
codon start: 1
label: pstS
note: pstS
translation: MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKWADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA039/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA039/annotation12/range/1

https://synbiohub.org/public/SEGA/SEGA039/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA039/1
start: 4989
end: 5236
orientation: inline

BCD
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
codon start: 1
label: BCD
note: BCD
translation: MKAIFVLKHLNHAKEVF
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA039/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA039/annotation6/range/1

https://synbiohub.org/public/SEGA/SEGA039/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA039/1
start: 2656
end: 2711
orientation: inline

Promoter 3BCD2
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
label: Promoter 3BCD2
note: Promoter 3BCD2
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA039/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA039/annotation9/range/1

https://synbiohub.org/public/SEGA/SEGA039/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA039/1
start: 3485
end: 3513
orientation: inline

rhaB promoter
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
label: rhaB promoter
note: promoter of the E. coli rhaBAD operon, conferring tight induction with L-rhamnose and repression with D-glucose in the presence of RhaR and RhaS (Giacalone et al., 2006)
note: rhaB promoter
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA039/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA039/annotation4/range/1

https://synbiohub.org/public/SEGA/SEGA039/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA039/1
start: 2508
end: 2626
orientation: inline

medium TIR
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
note: TIR
note: medium TIR
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA039/1
role: SO:0000139
location: https://synbiohub.org/public/SEGA/SEGA039/annotation5/range/1

https://synbiohub.org/public/SEGA/SEGA039/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA039/1
start: 2638
end: 2664
orientation: inline

https://synbiohub.org/public/SEGA/SEGA075/1
type: ComponentDefinition
persistent identity: SEGA075
display id: SEGA075
version: 1
description: natural linear DNA.
owned by: carbay
owned by: morno
date: 09-SEP-2021
division: UNA
keywords: .
locus: SEGA075
molecule: ds-DNA
organism: unspecified
reference: https://synbiohub.org/public/SEGA/reference0_1/1
source: natural DNA sequence
type: DnaRegion
type: SO:0000987
role: SO:0000804
sequence annotation: TcR
sequence annotation: NahR-AM
sequence annotation: GFP
sequence annotation: pstS
sequence annotation: Promoter 3BCD2
sequence annotation: L3S2P11 Terminator
sequence annotation: pLacIQ
sequence annotation: high TIR
sequence annotation: RiboJ
sequence annotation: L3S3P22 Terminator (synthetic)
sequence annotation: BCD
sequence annotation: glmS
sequence annotation: nah2
sequence annotation: pSalTTC
sequence annotation: rrnC
sequence: https://synbiohub.org/public/SEGA/SEGA075_seq/1

TcR
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
codon start: 1
gene: tet
label: TcR
note: TcR
note: confers resistance to tetracycline
product: tetracycline efflux protein
translation: MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHYGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVIFGEDRFRWSATMSGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA075/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA075/annotation12/range/1

https://synbiohub.org/public/SEGA/SEGA075/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA075/1
start: 2740
end: 3930
orientation: inline

NahR-AM
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
label: NahR-AM
note: NahR-AM
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA075/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA075/annotation2/range/1

https://synbiohub.org/public/SEGA/SEGA075/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA075/1
start: 611
end: 1513
orientation: reverseComplement

GFP
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
codon start: 1
label: GFP
note: GFP
product: green fluorescent protein
translation: SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYK
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA075/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA075/annotation9/range/1

https://synbiohub.org/public/SEGA/SEGA075/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA075/1
start: 1899
end: 2609
orientation: inline

pstS
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
codon start: 1
label: pstS
note: pstS
translation: MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKWADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA075/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA075/annotation14/range/1

https://synbiohub.org/public/SEGA/SEGA075/annotation14/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA075/1
start: 4176
end: 4423
orientation: inline

Promoter 3BCD2
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
label: Promoter 3BCD2
note: Promoter 3BCD2
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA075/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA075/annotation11/range/1

https://synbiohub.org/public/SEGA/SEGA075/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA075/1
start: 2672
end: 2700
orientation: inline

L3S2P11 Terminator
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
label: L3S2P11 Terminator
note: L3S2P11 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA075/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA075/annotation1/range/1

https://synbiohub.org/public/SEGA/SEGA075/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA075/1
start: 538
end: 594
orientation: inline

pLacIQ
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
label: pLacIQ
note: pLacIQ
note: promoter
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA075/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA075/annotation4/range/1

https://synbiohub.org/public/SEGA/SEGA075/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA075/1
start: 1543
end: 1596
orientation: reverseComplement

high TIR
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
note: TIR
note: high TIR
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA075/1
role: SO:0000139
location: https://synbiohub.org/public/SEGA/SEGA075/annotation7/range/1

https://synbiohub.org/public/SEGA/SEGA075/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA075/1
start: 1825
end: 1851
orientation: inline

RiboJ
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
label: RiboJ
note: RiboJ
note: insulator: self-cleaving ribozyme RiboJ
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA075/1
role: SO:0001411
location: https://synbiohub.org/public/SEGA/SEGA075/annotation6/range/1

https://synbiohub.org/public/SEGA/SEGA075/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA075/1
start: 1739
end: 1813
orientation: inline

L3S3P22 Terminator (synthetic)
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
label: L3S3P22 Terminator (synthetic)
note: L3S3P22 Terminator
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA075/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA075/annotation10/range/1

https://synbiohub.org/public/SEGA/SEGA075/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA075/1
start: 2613
end: 2661
orientation: inline

BCD
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
codon start: 1
label: BCD
note: BCD
translation: MKAIFVLKHLNHAKEVF
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA075/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA075/annotation8/range/1

https://synbiohub.org/public/SEGA/SEGA075/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA075/1
start: 1843
end: 1898
orientation: inline

glmS
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
codon start: 1
label: glmS
note: glmS
translation: VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA075/1
role: SO:0000316
location: https://synbiohub.org/public/SEGA/SEGA075/annotation0/range/1

https://synbiohub.org/public/SEGA/SEGA075/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA075/1
start: 1
end: 528
orientation: inline

nah2
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
label: nah2
note: nah2
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA075/1
role: SO:0000139
location: https://synbiohub.org/public/SEGA/SEGA075/annotation3/range/1

https://synbiohub.org/public/SEGA/SEGA075/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA075/1
start: 1514
end: 1542
orientation: inline

pSalTTC
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
label: pSalTTC
note: pSalTTC
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA075/1
role: SO:0000167
location: https://synbiohub.org/public/SEGA/SEGA075/annotation5/range/1

https://synbiohub.org/public/SEGA/SEGA075/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA075/1
start: 1623
end: 1738
orientation: inline

rrnC
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
label: rrnC
note: rrnC
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA075/1
role: SO:0000141
location: https://synbiohub.org/public/SEGA/SEGA075/annotation13/range/1

https://synbiohub.org/public/SEGA/SEGA075/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: carbay
owned by: morno
top level: https://synbiohub.org/public/SEGA/SEGA075/1
start: 3941
end: 3981
orientation: inline

https://synbiohub.org/public/SEGA/SEGA097_seq/1
type: Sequence
persistent identity: SEGA097 seq
display id: SEGA097_seq
version: 1
owned by: carbay
owned by: morno
elements: gtgcatggtctgcaggcgctgccgagccgtattgagcagatgctgtctcaggacaaacgcattgaagcgctggcagaagatttctctgacaaacatcacgcgctgttcctgggccgtggcgatcagtacccaatcgcgctggaaggcgcattgaagttgaaagagatctcttacattcacgctgaagcctacgctgctggcgaactgaaacacggtccgctggcgctaattgatgccgatatgccggttattgttgttgcaccgaacaacgaattgctggaaaaactgaaatccaacattgaagaagttcgcgcgcgtggcggtcagttgtatgtcttcgccgatcaggatgcgggttttgtaagtagcgataacatgcacatcatcgagatgccgcatgtggaagaggtgattgcaccgatcttctacaccgttccgctgcagctgctggcttaccatgtcgcgctgatcaaaggcaccgacgttgaccagccgcgtaacctggcaaaatcggttacggttgagtaataaatggatctcggtaccaaattccagaaaagagacgctttcgagcgtcttttttcgttttggtccgcgttgcgcttgacggctagctcagtcctaggtacagtgctagctactagagattaaagaggagaaactatatatgaaagcaattttcgtactgaaacatcttaatcatgctaaggaggttttctaatggtactgccgggcctcttgcgggatatcgtccattccgacagcatcgccagtcactatggcgtgctgctagcgctatatgcgttgatgcaatttctatgcgcacccgttctcggagcactgtccgaccgctttggccgccgcccagtcctgctcgcttcgctacttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggattctctacgccggacgcatcgtggccggcatcaccggcgccacaggtgcggttgctggcgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggcgccatctccctgcacgcaccattccttgcggcggcggtgctcaacggcctcaacctactactgggctgcttcctaatgcaggagtcgcataagggagagcgccgtccgatgcccttgagagccttcaacccagtcagctccttccggtgggcgcggggcatgaccattgtggccgcacttatgactgtcttctttatcatgcaactcgtaggacaggtgccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgagcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcactggtcccgccaccaaacgtttcggcgagaagcaggccattatcgccggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgaggctggatggccttccccattatgattcttctcgcttccggcggcatcgggatgcccgcgttgcaggccatgctgtccaggcaggtagatgacgaccatcagggacagcttcaaggatcgctcgcggctcttaccagcctaacttcgatcactggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggcgccgccctataccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctgagacaattgtccagataaaaaaaatccttagctttcgctaaggatgatttctaaaatttttctgtcatctcttcgttattaatgtttgtaattgactgaatatcaacgcttatttaaatcagactgaagactttatctctctgtcataaaactgtcatattcctt…
encoding: naseq.html

https://synbiohub.org/public/SEGA/SEGA028_seq/1
type: Sequence
persistent identity: SEGA028 seq
display id: SEGA028_seq
version: 1
owned by: carbay
owned by: morno
elements: gtgcatggtctgcaggcgctgccgagccgtattgagcagatgctgtctcaggacaaacgcattgaagcgctggcagaagatttctctgacaaacatcacgcgctgttcctgggccgtggcgatcagtacccaatcgcgctggaaggcgcattgaagttgaaagagatctcttacattcacgctgaagcctacgctgctggcgaactgaaacacggtccgctggcgctaattgatgccgatatgccggttattgttgttgcaccgaacaacgaattgctggaaaaactgaaatccaacattgaagaagttcgcgcgcgtggcggtcagttgtatgtcttcgccgatcaggatgcgggttttgtaagtagcgataacatgcacatcatcgagatgccgcatgtggaagaggtgattgcaccgatcttctacaccgttccgctgcagctgctggcttaccatgtcgcgctgatcaaaggcaccgacgttgaccagccgcgtaacctggcaaaatcggttacggttgagtaataaatggatctcggtaccaaattccagaaaagagacgctttcgagcgtcttttttcgttttggtccgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgccagggtggtttttcttttcaccagtgagacgggcaacagctgattgcccttcaccgcctggccctgagagagttgcagcaagcggtccacgctggtttgccccagcaggcgaaaatcctgtttgatggtggttaacggcgggatataacatgagctgtcttcggtatcgtcgtatcccactaccgagatgtccgcaccaacgcgcagcccggactcggtaatggcgcgcattgcgcccagcgccatctgatcgttggcaaccagcatcgcagtgggaacgatgccctcattcagcatttgcatggtttgttgaaaaccggacatggcactccagtcgccttcccgttccgctatcggctgaatttgattgcgagtgagatatttatgccagccagccagacgcagacgcgccgagacagaacttaatgggcccgctaacagcgcgatttgctggtgacccaatgcgaccagatgctccacgcccagtcgcgtaccgtcttcatgggagaaaataatactgttgatgggtgtctggtcagagacatcaagaaataacgccggaacattagtgcaggcagcttccacagcaatggcatcctggtcatccagcggatagttaatgatcagcccactgacgcgttgcgcgagaagattgtgcaccgccgctttacaggcttcgacgccgcttcgttctaccatcgacaccaccacgctggcacccagttgatcggcgcgagatttaatcgccgcgacaatttgcgacggcgcgtgcagggccagactggaggtggcaacgccaatcagcaacgactgtttgcccgccagttgttgtgccacgcggttgggaatgtaattcagctccgccatcgccgcttccactttttcccgcgttttcgcagaaacgtggctggcctggttcaccacgcgggaaacggtctgataagagacaccggcatactctgcgacatcgtataacgttactggtttcacattcaccaccctgaattgactctcttccgggcgctatcatgccataccgcgaaaggttttgcgccattcgatggtgtcggatcgagatcgatctcgatcccgcgaaatttgacaattaatcatccggctcgtataatggaattgtgagcggataacaattcctactagagattaaagaggagaaaatatcgatggagaacctgtacttccagagcaaaggagaagaacttttcactggagttgtgccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggt…
encoding: naseq.html

https://synbiohub.org/public/SEGA/SEGA100_seq/1
type: Sequence
persistent identity: SEGA100 seq
display id: SEGA100_seq
version: 1
owned by: carbay
owned by: morno
elements: gtgcatggtctgcaggcgctgccgagccgtattgagcagatgctgtctcaggacaaacgcattgaagcgctggcagaagatttctctgacaaacatcacgcgctgttcctgggccgtggcgatcagtacccaatcgcgctggaaggcgcattgaagttgaaagagatctcttacattcacgctgaagcctacgctgctggcgaactgaaacacggtccgctggcgctaattgatgccgatatgccggttattgttgttgcaccgaacaacgaattgctggaaaaactgaaatccaacattgaagaagttcgcgcgcgtggcggtcagttgtatgtcttcgccgatcaggatgcgggttttgtaagtagcgataacatgcacatcatcgagatgccgcatgtggaagaggtgattgcaccgatcttctacaccgttccgctgcagctgctggcttaccatgtcgcgctgatcaaaggcaccgacgttgaccagccgcgtaacctggcaaaatcggttacggttgagtaataaatggatctcggtaccaaattccagaaaagagacgctttcgagcgtcttttttcgttttggtccgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgccagggtggtttttcttttcaccagtgagacgggcaacagctgattgcccttcaccgcctggccctgagagagttgcagcaagcggtccacgctggtttgccccagcaggcgaaaatcctgtttgatggtggttaacggcgggatataacatgagctgtcttcggtatcgtcgtatcccactaccgagatgtccgcaccaacgcgcagcccggactcggtaatggcgcgcattgcgcccagcgccatctgatcgttggcaaccagcatcgcagtgggaacgatgccctcattcagcatttgcatggtttgttgaaaaccggacatggcactccagtcgccttcccgttccgctatcggctgaatttgattgcgagtgagatatttatgccagccagccagacgcagacgcgccgagacagaacttaatgggcccgctaacagcgcgatttgctggtgacccaatgcgaccagatgctccacgcccagtcgcgtaccgtcttcatgggagaaaataatactgttgatgggtgtctggtcagagacatcaagaaataacgccggaacattagtgcaggcagcttccacagcaatggcatcctggtcatccagcggatagttaatgatcagcccactgacgcgttgcgcgagaagattgtgcaccgccgctttacaggcttcgacgccgcttcgttctaccatcgacaccaccacgctggcacccagttgatcggcgcgagatttaatcgccgcgacaatttgcgacggcgcgtgcagggccagactggaggtggcaacgccaatcagcaacgactgtttgcccgccagttgttgtgccacgcggttgggaatgtaattcagctccgccatcgccgcttccactttttcccgcgttttcgcagaaacgtggctggcctggttcaccacgcgggaaacggtctgataagagacaccggcatactctgcgacatcgtataacgttactggtttcacattcaccaccctgaattgactctcttccgggcgctatcatgccataccgcgaaaggttttgcgccattcgatggtgtcggatcgagatcgatctcgatcccgcgaaatttgacaattaatcatccggctcgtataatggaattgtgagcggataacaattcctactagagattaaagaggagaaactatatatgaaagcaattttcgtactgaaacatcttaatcatgctaaggaggttttctaatggtactgccgggcctcttgcgggatatcgtccattccgacagcatcgccagtcactatggcgtgctgc…
encoding: naseq.html

https://synbiohub.org/public/SEGA/SEGA016_seq/1
type: Sequence
persistent identity: SEGA016 seq
display id: SEGA016_seq
version: 1
owned by: carbay
owned by: morno
elements: gtgcatggtctgcaggcgctgccgagccgtattgagcagatgctgtctcaggacaaacgcattgaagcgctggcagaagatttctctgacaaacatcacgcgctgttcctgggccgtggcgatcagtacccaatcgcgctggaaggcgcattgaagttgaaagagatctcttacattcacgctgaagcctacgctgctggcgaactgaaacacggtccgctggcgctaattgatgccgatatgccggttattgttgttgcaccgaacaacgaattgctggaaaaactgaaatccaacattgaagaagttcgcgcgcgtggcggtcagttgtatgtcttcgccgatcaggatgcgggttttgtaagtagcgataacatgcacatcatcgagatgccgcatgtggaagaggtgattgcaccgatcttctacaccgttccgctgcagctgctggcttaccatgtcgcgctgatcaaaggcaccgacgttgaccagccgcgtaacctggcaaaatcggttacggttgagtaataaatggatctcggtaccaaattccagaaaagagacgctttcgagcgtcttttttcgttttggtccgcgttgcgcttgacggctagctcagtcctaggtacagtgctagctactagagattaaagaggagaaaatatcgatggagaacctgtacttccagtttcgtagcaaaggagaagaacttgtcactggagttagcaaaggagaagaacttttcactggagttgtgccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgctacatacggaaaactcacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactctgacctatggcgttcaatgcttttcccgttatccggatcacatgaaacggcatgactttttcaagagtgccatgcccgaaggttatgtacaggaacgcactatatctttcaaagatgacgggaactacaagacgcgtgctgaagtcaagtttgaaggtgatacccttgttaatcgtatcgagttaaagggtattgattttaaagaagatggaaacattctcggacacaaactagagtacaactataactcacacaatgtatacatcacggcagacaaacaaaagaatggaatcaaagctaacttcaaaattcgccacaacattgaagatggttccgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtcgacacaatctgccctttcgaaagatcccaacgaaaagcgtgaccacatggtccttcttgagtttgtaactgctgctgggattacacatggcatggatgagctctacaaatgaccaattattgaaggccgctaacgcggcctttttttgtttctggtctcccaaaaatttatttgcttattaatcatccggctcgtataatgtgtggagggcccaagttcacttaaaaaggagaatagttatgaaatcgaacaatgcgctcatcgtcatcctcggcaccgtcaccctggatgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagcatcgccagtcactatggcgtgctgctagcgctatatgcgttgatgcaatttctatgcgcacccgttctcggagcactgtccgaccgctttggccgccgcccagtcctgctcgcttcgctacttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggattctctacgccggacgcatcgtggccggcatcaccggcgccacaggtgcggttgctggcgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtg…
encoding: naseq.html

https://synbiohub.org/public/SEGA/SEGA089_seq/1
type: Sequence
persistent identity: SEGA089 seq
display id: SEGA089_seq
version: 1
owned by: carbay
owned by: morno
elements: gtgcatggtctgcaggcgctgccgagccgtattgagcagatgctgtctcaggacaaacgcattgaagcgctggcagaagatttctctgacaaacatcacgcgctgttcctgggccgtggcgatcagtacccaatcgcgctggaaggcgcattgaagttgaaagagatctcttacattcacgctgaagcctacgctgctggcgaactgaaacacggtccgctggcgctaattgatgccgatatgccggttattgttgttgcaccgaacaacgaattgctggaaaaactgaaatccaacattgaagaagttcgcgcgcgtggcggtcagttgtatgtcttcgccgatcaggatgcgggttttgtaagtagcgataacatgcacatcatcgagatgccgcatgtggaagaggtgattgcaccgatcttctacaccgttccgctgcagctgctggcttaccatgtcgcgctgatcaaaggcaccgacgttgaccagccgcgtaacctggcaaaatcggttacggttgagtaataaatggatctcggtaccaaattccagaaaagagacgctttcgagcgtcttttttcgttttggtccaccgtattcatcagcactgtcctgctccttgtgatggtttacaaacgtaaaaagtctctttaatacctgtttttgcttcatattgttcagcgacagcttgctgtacggcaggcaccagctcttccgggatcagcgcgacgatacagccgccaaatccgccgccggtcatgcgtacgccacctttgtcgccaatcacagctttgacgatttctaccagagtgtcaatttgcggcacggtgatttcgaaatcatcgcgcatagaggcatgagactccgccatcaactcgcccatacgtttcaggtcgccttgctccagcgcgctggcagcttcaacggtgcgggcgttttcagtcagtatatgacgcacgcgttttgccacgatcgggtccagttcatgcgcaacagcgttgaactcttcaatggtgacatcacgcagggctggctgctggaagaaacgcgcaccggtttcgcactgttcacgacgggtgttgtattcgctgccaaccagggtacgtttgaagttactgttgatgatgacgacagccacacctttgggcatggaaactgctttggtccccagtgagcggcaatcgatcagcaaggcatgatctttcttgccgagcgcggaaattagctgatccatgatcccgcagttacagcctacaaactggttttctgcttcctgaccgttaagcgcgatttgtgcgccgtccagcggcagatgataaagctgctgcaatacggttccgaccgcgacttccagtgaagcggaagaacttaacccggcaccctgcggcacattgccgctgatcaccatgtccacgccgccgaagctgttgttacgcagttgcagatgtttcaccacgccacgaacgtagttagcccattgatagttttcatgtgcgacaatgggcgcatcgagggaaaactcgtcgagctgattttcataatcggctgccatcacgcgaactttacggtcatcgcgtggtgcacaactgatcacggtttgataatcaatcgcgcagggcagaacgaaaccgtcgttgtagtcggtgtgttcaccaatcaaattcacgcggccaggcgcctgaatggtgtgagtggcagggtagccaaatgcgttggcaaacagagattgtgttttttctttcagactcatgattgcctcctgggttagttcctcaccttgtcgtattatactatgccgatatactatgccgatgattaattgtcaacctttaatgaattataaaacgaaaggctcagtcgaaagactgggcctttcgttttatgcgttgcgcttaatctttctgcgaattgagatgacgccactggctgggcgtcatcccggtttcccgggtaaacaccaccgaaaaatagttactatcttcaaagccacattcggt…
encoding: naseq.html

https://synbiohub.org/public/SEGA/SEGA044_seq/1
type: Sequence
persistent identity: SEGA044 seq
display id: SEGA044_seq
version: 1
owned by: carbay
owned by: morno
elements: gtgcatggtctgcaggcgctgccgagccgtattgagcagatgctgtctcaggacaaacgcattgaagcgctggcagaagatttctctgacaaacatcacgcgctgttcctgggccgtggcgatcagtacccaatcgcgctggaaggcgcattgaagttgaaagagatctcttacattcacgctgaagcctacgctgctggcgaactgaaacacggtccgctggcgctaattgatgccgatatgccggttattgttgttgcaccgaacaacgaattgctggaaaaactgaaatccaacattgaagaagttcgcgcgcgtggcggtcagttgtatgtcttcgccgatcaggatgcgggttttgtaagtagcgataacatgcacatcatcgagatgccgcatgtggaagaggtgattgcaccgatcttctacaccgttccgctgcagctgctggcttaccatgtcgcgctgatcaaaggcaccgacgttgaccagccgcgtaacctggcaaaatcggttacggttgagtaataaatggatctcggtaccaaattccagaaaagagacgctttcgagcgtcttttttcgttttggtccgcgttgcgcttaatctttctgcgaattgagatgacgccactggctgggcgtcatcccggtttcccgggtaaacaccaccgaaaaatagttactatcttcaaagccacattcggtcgaaatatcactgattaacaggcggctatgctggagaagatattgcgcatgacacactctgacctgtcgcagatattgattgatggtcattccagtctgctggcgaaattgctgacgcaaaacgcgctcactgcacgatgcctcatcacaaaatttatccagcgcaaagggacttttcaggctagccgccagccgggtaatcagcttatccagcaacgtttcgctggatgttggcggcaacgaatcactggtgtaacgatggcgattcagcaacatcaccaactgcccgaacagcaactcagccatttcgttagcaaacggcacatgctgactactttcatgctcaagctgaccaataacctgccgcgcctgcgccatccccatgctacctaagcgccagtgtggttgccctgcgctggcgttaaatcccggaatcgccccctgccagtcaagattcagcttcagacgctccgggcaataaataatattctgcaaaaccagatcgttaacggaagcgtaggagtgtttatcatcagcatgaatgtaaaagagatcgccacgggtaatgcgataagggcgatcgttgagtacatgcaggccattaccgcgccagacaatcaccagctcacaaaaatcatgtgtatgttcagcaaagacatcttgcggataacggtcagccacagcgactgcctgctggtcgctggcaaaaaaatcatctttgagaagttttaactgatgcgccaccgtggctacctcggccagagaacgaagttgattattcgcaatatggcgtacaaatacgttgagaagattcgcgttattgcagaaagccatcccgtccctggcgaatatcacgcggtgaccagttaaactctcggcgaaaaagcgtcgaaaagtggttactgtcgctgaatccacagcgataggcgatgtcagtaacgctggcctcgctgtggcgtagcagatgtcgggctttcatcagtcgcaggcggttcaggtatcgctgaggcgtcagtcccgtttgctgcttaagctgccgatgtagcgtacgcagtgaaagagaaaattgatccgccacggcatcccaattcacctcatcggcaaaatggtcctccagccaggccagaagcaagttgagacgtgatgcgctgttttccaggttctcctgcaaactgcttttacgcagcaagagcagtaattgcataaacaagatctcgcgactggcggtcgagggtaaatcattttccccttcctgctgttccatctgtgcaaccagctgtcgcacctgctgc…
encoding: naseq.html

https://synbiohub.org/public/SEGA/SEGA029_seq/1
type: Sequence
persistent identity: SEGA029 seq
display id: SEGA029_seq
version: 1
owned by: carbay
owned by: morno
elements: gtgcatggtctgcaggcgctgccgagccgtattgagcagatgctgtctcaggacaaacgcattgaagcgctggcagaagatttctctgacaaacatcacgcgctgttcctgggccgtggcgatcagtacccaatcgcgctggaaggcgcattgaagttgaaagagatctcttacattcacgctgaagcctacgctgctggcgaactgaaacacggtccgctggcgctaattgatgccgatatgccggttattgttgttgcaccgaacaacgaattgctggaaaaactgaaatccaacattgaagaagttcgcgcgcgtggcggtcagttgtatgtcttcgccgatcaggatgcgggttttgtaagtagcgataacatgcacatcatcgagatgccgcatgtggaagaggtgattgcaccgatcttctacaccgttccgctgcagctgctggcttaccatgtcgcgctgatcaaaggcaccgacgttgaccagccgcgtaacctggcaaaatcggttacggttgagtaataaatggatctcggtaccaaattccagaaaagagacgctttcgagcgtcttttttcgttttggtccgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgccagggtggtttttcttttcaccagtgagacgggcaacagctgattgcccttcaccgcctggccctgagagagttgcagcaagcggtccacgctggtttgccccagcaggcgaaaatcctgtttgatggtggttaacggcgggatataacatgagctgtcttcggtatcgtcgtatcccactaccgagatgtccgcaccaacgcgcagcccggactcggtaatggcgcgcattgcgcccagcgccatctgatcgttggcaaccagcatcgcagtgggaacgatgccctcattcagcatttgcatggtttgttgaaaaccggacatggcactccagtcgccttcccgttccgctatcggctgaatttgattgcgagtgagatatttatgccagccagccagacgcagacgcgccgagacagaacttaatgggcccgctaacagcgcgatttgctggtgacccaatgcgaccagatgctccacgcccagtcgcgtaccgtcttcatgggagaaaataatactgttgatgggtgtctggtcagagacatcaagaaataacgccggaacattagtgcaggcagcttccacagcaatggcatcctggtcatccagcggatagttaatgatcagcccactgacgcgttgcgcgagaagattgtgcaccgccgctttacaggcttcgacgccgcttcgttctaccatcgacaccaccacgctggcacccagttgatcggcgcgagatttaatcgccgcgacaatttgcgacggcgcgtgcagggccagactggaggtggcaacgccaatcagcaacgactgtttgcccgccagttgttgtgccacgcggttgggaatgtaattcagctccgccatcgccgcttccactttttcccgcgttttcgcagaaacgtggctggcctggttcaccacgcgggaaacggtctgataagagacaccggcatactctgcgacatcgtataacgttactggtttcacattcaccaccctgaattgactctcttccgggcgctatcatgccataccgcgaaaggttttgcgccattcgatggtgtcggatcgagatcgatctcgatcccgcgaaatttgacaattaatcatccggctcgtataatggaattgtgagcggataacaattcctactagagattaaagaggagaaacgggggatggagaacctgtacttccagtttcgtagcaaaggagaagaacttgtcactggagttagcaaaggagaagaacttttcactggagttgtgccaattcttgttgaattagatggtgatgttaat…
encoding: naseq.html

https://synbiohub.org/public/SEGA/SEGA005_seq/1
type: Sequence
persistent identity: SEGA005 seq
display id: SEGA005_seq
version: 1
owned by: carbay
owned by: morno
elements: gtgcatggtctgcaggcgctgccgagccgtattgagcagatgctgtctcaggacaaacgcattgaagcgctggcagaagatttctctgacaaacatcacgcgctgttcctgggccgtggcgatcagtacccaatcgcgctggaaggcgcattgaagttgaaagagatctcttacattcacgctgaagcctacgctgctggcgaactgaaacacggtccgctggcgctaattgatgccgatatgccggttattgttgttgcaccgaacaacgaattgctggaaaaactgaaatccaacattgaagaagttcgcgcgcgtggcggtcagttgtatgtcttcgccgatcaggatgcgggttttgtaagtagcgataacatgcacatcatcgagatgccgcatgtggaagaggtgattgcaccgatcttctacaccgttccgctgcagctgctggcttaccatgtcgcgctgatcaaaggcaccgacgttgaccagccgcgtaacctggcaaaatcggttacggttgagtaataaatggatctcggtaccaaattccagaaaagagacgctttcgagcgtcttttttcgttttggtccgcgttgcgcttgacggctagctcagtcctaggtacagtgctagctactagagattaaagaggagaaaggggggatgaaggctattttcgtactgaaacatcttaatcatgctaaggaggttttctaatgagcaaaggagaagaacttttcactggagttgtgccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgctacatacggaaaactcacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactctgacctatggcgttcaatgcttttcccgttatccggatcacatgaaacggcatgactttttcaagagtgccatgcccgaaggttatgtacaggaacgcactatatctttcaaagatgacgggaactacaagacgcgtgctgaagtcaagtttgaaggtgatacccttgttaatcgtatcgagttaaagggtattgattttaaagaagatggaaacattctcggacacaaactagagtacaactataactcacacaatgtatacatcacggcagacaaacaaaagaatggaatcaaagctaacttcaaaattcgccacaacattgaagatggttccgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtcgacacaatctgccctttcgaaagatcccaacgaaaagcgtgaccacatggtccttcttgagtttgtaactgctgctgggattacacatggcatggatgagctctacaaatgaccaattattgaaggccgctaacgcggcctttttttgtttctggtctcccaaaaatttatttgcttattaatcatccggctcgtataatgtgtggagggcccaagttcacttaaaaaggagaatagttatgaaatcgaacaatgcgctcatcgtcatcctcggcaccgtcaccctggatgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagcatcgccagtcactatggcgtgctgctagcgctatatgcgttgatgcaatttctatgcgcacccgttctcggagcactgtccgaccgctttggccgccgcccagtcctgctcgcttcgctacttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggattctctacgccggacgcatcgtggccggcatcaccggcgccacaggtgcggttgctggcgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtgg…
encoding: naseq.html

https://synbiohub.org/public/SEGA/SEGA031_seq/1
type: Sequence
persistent identity: SEGA031 seq
display id: SEGA031_seq
version: 1
owned by: carbay
owned by: morno
elements: gtgcatggtctgcaggcgctgccgagccgtattgagcagatgctgtctcaggacaaacgcattgaagcgctggcagaagatttctctgacaaacatcacgcgctgttcctgggccgtggcgatcagtacccaatcgcgctggaaggcgcattgaagttgaaagagatctcttacattcacgctgaagcctacgctgctggcgaactgaaacacggtccgctggcgctaattgatgccgatatgccggttattgttgttgcaccgaacaacgaattgctggaaaaactgaaatccaacattgaagaagttcgcgcgcgtggcggtcagttgtatgtcttcgccgatcaggatgcgggttttgtaagtagcgataacatgcacatcatcgagatgccgcatgtggaagaggtgattgcaccgatcttctacaccgttccgctgcagctgctggcttaccatgtcgcgctgatcaaaggcaccgacgttgaccagccgcgtaacctggcaaaatcggttacggttgagtaataaatggatctcggtaccaaattccagaaaagagacgctttcgagcgtcttttttcgttttggtccgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgccagggtggtttttcttttcaccagtgagacgggcaacagctgattgcccttcaccgcctggccctgagagagttgcagcaagcggtccacgctggtttgccccagcaggcgaaaatcctgtttgatggtggttaacggcgggatataacatgagctgtcttcggtatcgtcgtatcccactaccgagatgtccgcaccaacgcgcagcccggactcggtaatggcgcgcattgcgcccagcgccatctgatcgttggcaaccagcatcgcagtgggaacgatgccctcattcagcatttgcatggtttgttgaaaaccggacatggcactccagtcgccttcccgttccgctatcggctgaatttgattgcgagtgagatatttatgccagccagccagacgcagacgcgccgagacagaacttaatgggcccgctaacagcgcgatttgctggtgacccaatgcgaccagatgctccacgcccagtcgcgtaccgtcttcatgggagaaaataatactgttgatgggtgtctggtcagagacatcaagaaataacgccggaacattagtgcaggcagcttccacagcaatggcatcctggtcatccagcggatagttaatgatcagcccactgacgcgttgcgcgagaagattgtgcaccgccgctttacaggcttcgacgccgcttcgttctaccatcgacaccaccacgctggcacccagttgatcggcgcgagatttaatcgccgcgacaatttgcgacggcgcgtgcagggccagactggaggtggcaacgccaatcagcaacgactgtttgcccgccagttgttgtgccacgcggttgggaatgtaattcagctccgccatcgccgcttccactttttcccgcgttttcgcagaaacgtggctggcctggttcaccacgcgggaaacggtctgataagagacaccggcatactctgcgacatcgtataacgttactggtttcacattcaccaccctgaattgactctcttccgggcgctatcatgccataccgcgaaaggttttgcgccattcgatggtgtcggatcgagatcgatctcgatcccgcgaaatttgacaattaatcatccggctcgtataatggaattgtgagcggataacaattcctactagagattaaagaggagaaaatatcgatggagaacctgtacttccagtttcgtagcaaaggagaagaacttgtcactggagttagcaaaggagaagaacttttcactggagttgtgccaattcttgttgaattagatggtgatgttaat…
encoding: naseq.html

https://synbiohub.org/public/SEGA/SEGA087_seq/1
type: Sequence
persistent identity: SEGA087 seq
display id: SEGA087_seq
version: 1
owned by: carbay
owned by: morno
elements: gtgcatggtctgcaggcgctgccgagccgtattgagcagatgctgtctcaggacaaacgcattgaagcgctggcagaagatttctctgacaaacatcacgcgctgttcctgggccgtggcgatcagtacccaatcgcgctggaaggcgcattgaagttgaaagagatctcttacattcacgctgaagcctacgctgctggcgaactgaaacacggtccgctggcgctaattgatgccgatatgccggttattgttgttgcaccgaacaacgaattgctggaaaaactgaaatccaacattgaagaagttcgcgcgcgtggcggtcagttgtatgtcttcgccgatcaggatgcgggttttgtaagtagcgataacatgcacatcatcgagatgccgcatgtggaagaggtgattgcaccgatcttctacaccgttccgctgcagctgctggcttaccatgtcgcgctgatcaaaggcaccgacgttgaccagccgcgtaacctggcaaaatcggttacggttgagtaataaatggatctcggtaccaaattccagaaaagagacgctttcgagcgtcttttttcgttttggtccaccgtattcatcagcactgtcctgctccttgtgatggtttacaaacgtaaaaagtctctttaatacctgtttttgcttcatattgttcagcgacagcttgctgtacggcaggcaccagctcttccgggatcagcgcgacgatacagccgccaaatccgccgccggtcatgcgtacgccacctttgtcgccaatcacagctttgacgatttctaccagagtgtcaatttgcggcacggtgatttcgaaatcatcgcgcatagaggcatgagactccgccatcaactcgcccatacgtttcaggtcgccttgctccagcgcgctggcagcttcaacggtgcgggcgttttcagtcagtatatgacgcacgcgttttgccacgatcgggtccagttcatgcgcaacagcgttgaactcttcaatggtgacatcacgcagggctggctgctggaagaaacgcgcaccggtttcgcactgttcacgacgggtgttgtattcgctgccaaccagggtacgtttgaagttactgttgatgatgacgacagccacacctttgggcatggaaactgctttggtccccagtgagcggcaatcgatcagcaaggcatgatctttcttgccgagcgcggaaattagctgatccatgatcccgcagttacagcctacaaactggttttctgcttcctgaccgttaagcgcgatttgtgcgccgtccagcggcagatgataaagctgctgcaatacggttccgaccgcgacttccagtgaagcggaagaacttaacccggcaccctgcggcacattgccgctgatcaccatgtccacgccgccgaagctgttgttacgcagttgcagatgtttcaccacgccacgaacgtagttagcccattgatagttttcatgtgcgacaatgggcgcatcgagggaaaactcgtcgagctgattttcataatcggctgccatcacgcgaactttacggtcatcgcgtggtgcacaactgatcacggtttgataatcaatcgcgcagggcagaacgaaaccgtcgttgtagtcggtgtgttcaccaatcaaattcacgcggccaggcgcctgaatggtgtgagtggcagggtagccaaatgcgttggcaaacagagattgtgttttttctttcagactcatgattgcctcctgggttagttcctcaccttgtcgtattatactatgccgatatactatgccgatgattaattgtcaacctttaatgaattataaaacgaaaggctcagtcgaaagactgggcctttcgttttatgcgttgcgcttaatctttctgcgaattgagatgacgccactggctgggcgtcatcccggtttcccgggtaaacaccaccgaaaaatagttactatcttcaaagccacattcggt…
encoding: naseq.html

https://synbiohub.org/public/SEGA/SEGA034_seq/1
type: Sequence
persistent identity: SEGA034 seq
display id: SEGA034_seq
version: 1
owned by: carbay
owned by: morno
elements: gtgcatggtctgcaggcgctgccgagccgtattgagcagatgctgtctcaggacaaacgcattgaagcgctggcagaagatttctctgacaaacatcacgcgctgttcctgggccgtggcgatcagtacccaatcgcgctggaaggcgcattgaagttgaaagagatctcttacattcacgctgaagcctacgctgctggcgaactgaaacacggtccgctggcgctaattgatgccgatatgccggttattgttgttgcaccgaacaacgaattgctggaaaaactgaaatccaacattgaagaagttcgcgcgcgtggcggtcagttgtatgtcttcgccgatcaggatgcgggttttgtaagtagcgataacatgcacatcatcgagatgccgcatgtggaagaggtgattgcaccgatcttctacaccgttccgctgcagctgctggcttaccatgtcgcgctgatcaaaggcaccgacgttgaccagccgcgtaacctggcaaaatcggttacggttgagtaataaatggatctcggtaccaaattccagaaaagagacgctttcgagcgtcttttttcgttttggtccgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgccagggtggtttttcttttcaccagtgagacgggcaacagctgattgcccttcaccgcctggccctgagagagttgcagcaagcggtccacgctggtttgccccagcaggcgaaaatcctgtttgatggtggttaacggcgggatataacatgagctgtcttcggtatcgtcgtatcccactaccgagatgtccgcaccaacgcgcagcccggactcggtaatggcgcgcattgcgcccagcgccatctgatcgttggcaaccagcatcgcagtgggaacgatgccctcattcagcatttgcatggtttgttgaaaaccggacatggcactccagtcgccttcccgttccgctatcggctgaatttgattgcgagtgagatatttatgccagccagccagacgcagacgcgccgagacagaacttaatgggcccgctaacagcgcgatttgctggtgacccaatgcgaccagatgctccacgcccagtcgcgtaccgtcttcatgggagaaaataatactgttgatgggtgtctggtcagagacatcaagaaataacgccggaacattagtgcaggcagcttccacagcaatggcatcctggtcatccagcggatagttaatgatcagcccactgacgcgttgcgcgagaagattgtgcaccgccgctttacaggcttcgacgccgcttcgttctaccatcgacaccaccacgctggcacccagttgatcggcgcgagatttaatcgccgcgacaatttgcgacggcgcgtgcagggccagactggaggtggcaacgccaatcagcaacgactgtttgcccgccagttgttgtgccacgcggttgggaatgtaattcagctccgccatcgccgcttccactttttcccgcgttttcgcagaaacgtggctggcctggttcaccacgcgggaaacggtctgataagagacaccggcatactctgcgacatcgtataacgttactggtttcacattcaccaccctgaattgactctcttccgggcgctatcatgccataccgcgaaaggttttgcgccattcgatggtgtcggatcgagatcgatctcgatcccgcgaaatttgacaattaatcatccggctcgtataatggaattgtgagcggataacaattcctactagagattaaagaggagaaattaattatgaaatatctgctgccgaccgctgctgctggtctgctgctcctcgctgcccagccggcgatggcccgtaaaggcgaagagctgttcactggtgtcgtccctattctggtggaactggatggt…
encoding: naseq.html

https://synbiohub.org/public/SEGA/SEGA014_seq/1
type: Sequence
persistent identity: SEGA014 seq
display id: SEGA014_seq
version: 1
owned by: carbay
owned by: morno
elements: gtgcatggtctgcaggcgctgccgagccgtattgagcagatgctgtctcaggacaaacgcattgaagcgctggcagaagatttctctgacaaacatcacgcgctgttcctgggccgtggcgatcagtacccaatcgcgctggaaggcgcattgaagttgaaagagatctcttacattcacgctgaagcctacgctgctggcgaactgaaacacggtccgctggcgctaattgatgccgatatgccggttattgttgttgcaccgaacaacgaattgctggaaaaactgaaatccaacattgaagaagttcgcgcgcgtggcggtcagttgtatgtcttcgccgatcaggatgcgggttttgtaagtagcgataacatgcacatcatcgagatgccgcatgtggaagaggtgattgcaccgatcttctacaccgttccgctgcagctgctggcttaccatgtcgcgctgatcaaaggcaccgacgttgaccagccgcgtaacctggcaaaatcggttacggttgagtaataaatggatctcggtaccaaattccagaaaagagacgctttcgagcgtcttttttcgttttggtccgcgttgcgcttgacggctagctcagtcctaggtacagtgctagctactagagattaaagaggagaaacgggggatggagaacctgtacttccagtttcgtagcaaaggagaagaacttgtcactggagttagcaaaggagaagaacttttcactggagttgtgccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgctacatacggaaaactcacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactctgacctatggcgttcaatgcttttcccgttatccggatcacatgaaacggcatgactttttcaagagtgccatgcccgaaggttatgtacaggaacgcactatatctttcaaagatgacgggaactacaagacgcgtgctgaagtcaagtttgaaggtgatacccttgttaatcgtatcgagttaaagggtattgattttaaagaagatggaaacattctcggacacaaactagagtacaactataactcacacaatgtatacatcacggcagacaaacaaaagaatggaatcaaagctaacttcaaaattcgccacaacattgaagatggttccgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtcgacacaatctgccctttcgaaagatcccaacgaaaagcgtgaccacatggtccttcttgagtttgtaactgctgctgggattacacatggcatggatgagctctacaaatgaccaattattgaaggccgctaacgcggcctttttttgtttctggtctcccaaaaatttatttgcttattaatcatccggctcgtataatgtgtggagggcccaagttcacttaaaaaggagaatagttatgaaatcgaacaatgcgctcatcgtcatcctcggcaccgtcaccctggatgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagcatcgccagtcactatggcgtgctgctagcgctatatgcgttgatgcaatttctatgcgcacccgttctcggagcactgtccgaccgctttggccgccgcccagtcctgctcgcttcgctacttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggattctctacgccggacgcatcgtggccggcatcaccggcgccacaggtgcggttgctggcgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtg…
encoding: naseq.html

https://synbiohub.org/public/SEGA/SEGA062_seq/1
type: Sequence
persistent identity: SEGA062 seq
display id: SEGA062_seq
version: 1
owned by: carbay
owned by: morno
elements: gtgcatggtctgcaggcgctgccgagccgtattgagcagatgctgtctcaggacaaacgcattgaagcgctggcagaagatttctctgacaaacatcacgcgctgttcctgggccgtggcgatcagtacccaatcgcgctggaaggcgcattgaagttgaaagagatctcttacattcacgctgaagcctacgctgctggcgaactgaaacacggtccgctggcgctaattgatgccgatatgccggttattgttgttgcaccgaacaacgaattgctggaaaaactgaaatccaacattgaagaagttcgcgcgcgtggcggtcagttgtatgtcttcgccgatcaggatgcgggttttgtaagtagcgataacatgcacatcatcgagatgccgcatgtggaagaggtgattgcaccgatcttctacaccgttccgctgcagctgctggcttaccatgtcgcgctgatcaaaggcaccgacgttgaccagccgcgtaacctggcaaaatcggttacggttgagtaataaatggatctcggtaccaaattccagaaaagagacgctttcgagcgtcttttttcgttttggtccgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgccagggtggtttttcttttcaccagtgagacgggcaacagctgattgcccttcaccgcctggccctgagagagttgcagcaagcggtccacgctggtttgccccagcaggcgaaaatcctgtttgatggtggttaacggcgggatataacatgagctgtcttcggtatcgtcgtatcccactaccgagatgtccgcaccaacgcgcagcccggactcggtaatggcgcgcattgcgcccagcgccatctgatcgttggcaaccagcatcgcagtgggaacgatgccctcattcagcatttgcatggtttgttgaaaaccggacatggcactccagtcgccttcccgttccgctatcggctgaatttgattgcgagtgagatatttatgccagccagccagacgcagacgcgccgagacagaacttaatgggcccgctaacagcgcgatttgctggtgacccaatgcgaccagatgctccacgcccagtcgcgtaccgtcttcatgggagaaaataatactgttgatgggtgtctggtcagagacatcaagaaataacgccggaacattagtgcaggcagcttccacagcaatggcatcctggtcatccagcggatagttaatgatcagcccactgacgcgttgcgcgagaagattgtgcaccgccgctttacaggcttcgacgccgcttcgttctaccatcgacaccaccacgctggcacccagttgatcggcgcgagatttaatcgccgcgacaatttgcgacggcgcgtgcagggccagactggaggtggcaacgccaatcagcaacgactgtttgcccgccagttgttgtgccacgcggttgggaatgtaattcagctccgccatcgccgcttccactttttcccgcgttttcgcagaaacgtggctggcctggttcaccacgcgggaaacggtctgataagagacaccggcatactctgcgacatcgtataacgttactggtttcacattcaccaccctgaattgactctcttccgggcgctatcatgccataccgcgaaaggttttgcgccattcgatggtgtcggatcgagatcgatctcgatcccgcgaaattaatacgactcactataggggaattgtgagcggataacaattcctactagagattaaagaggagaaacgggggatggagaacctgtacttccagagcaaaggagaagaacttttcactggagttgtgccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgctacat…
encoding: naseq.html

https://synbiohub.org/public/SEGA/SEGA007_seq/1
type: Sequence
persistent identity: SEGA007 seq
display id: SEGA007_seq
version: 1
owned by: carbay
owned by: morno
elements: gtgcatggtctgcaggcgctgccgagccgtattgagcagatgctgtctcaggacaaacgcattgaagcgctggcagaagatttctctgacaaacatcacgcgctgttcctgggccgtggcgatcagtacccaatcgcgctggaaggcgcattgaagttgaaagagatctcttacattcacgctgaagcctacgctgctggcgaactgaaacacggtccgctggcgctaattgatgccgatatgccggttattgttgttgcaccgaacaacgaattgctggaaaaactgaaatccaacattgaagaagttcgcgcgcgtggcggtcagttgtatgtcttcgccgatcaggatgcgggttttgtaagtagcgataacatgcacatcatcgagatgccgcatgtggaagaggtgattgcaccgatcttctacaccgttccgctgcagctgctggcttaccatgtcgcgctgatcaaaggcaccgacgttgaccagccgcgtaacctggcaaaatcggttacggttgagtaataaatggatctcggtaccaaattccagaaaagagacgctttcgagcgtcttttttcgttttggtccgcgttgcgcttgacggctagctcagtcctaggtacagtgctagctactagagattaaagaggagaaactatatatgaaagcaattttcgtactgaaacatcttaatcatgctaaggaggttttctaatgagcaaaggagaagaacttttcactggagttgtgccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgctacatacggaaaactcacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactctgacctatggcgttcaatgcttttcccgttatccggatcacatgaaacggcatgactttttcaagagtgccatgcccgaaggttatgtacaggaacgcactatatctttcaaagatgacgggaactacaagacgcgtgctgaagtcaagtttgaaggtgatacccttgttaatcgtatcgagttaaagggtattgattttaaagaagatggaaacattctcggacacaaactagagtacaactataactcacacaatgtatacatcacggcagacaaacaaaagaatggaatcaaagctaacttcaaaattcgccacaacattgaagatggttccgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtcgacacaatctgccctttcgaaagatcccaacgaaaagcgtgaccacatggtccttcttgagtttgtaactgctgctgggattacacatggcatggatgagctctacaaatgaccaattattgaaggccgctaacgcggcctttttttgtttctggtctcccaaaaatttatttgcttattaatcatccggctcgtataatgtgtggagggcccaagttcacttaaaaaggagaatagttatgaaatcgaacaatgcgctcatcgtcatcctcggcaccgtcaccctggatgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagcatcgccagtcactatggcgtgctgctagcgctatatgcgttgatgcaatttctatgcgcacccgttctcggagcactgtccgaccgctttggccgccgcccagtcctgctcgcttcgctacttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggattctctacgccggacgcatcgtggccggcatcaccggcgccacaggtgcggttgctggcgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtgg…
encoding: naseq.html

https://synbiohub.org/public/SEGA/SEGA071_seq/1
type: Sequence
persistent identity: SEGA071 seq
display id: SEGA071_seq
version: 1
owned by: carbay
owned by: morno
elements: gtgcatggtctgcaggcgctgccgagccgtattgagcagatgctgtctcaggacaaacgcattgaagcgctggcagaagatttctctgacaaacatcacgcgctgttcctgggccgtggcgatcagtacccaatcgcgctggaaggcgcattgaagttgaaagagatctcttacattcacgctgaagcctacgctgctggcgaactgaaacacggtccgctggcgctaattgatgccgatatgccggttattgttgttgcaccgaacaacgaattgctggaaaaactgaaatccaacattgaagaagttcgcgcgcgtggcggtcagttgtatgtcttcgccgatcaggatgcgggttttgtaagtagcgataacatgcacatcatcgagatgccgcatgtggaagaggtgattgcaccgatcttctacaccgttccgctgcagctgctggcttaccatgtcgcgctgatcaaaggcaccgacgttgaccagccgcgtaacctggcaaaatcggttacggttgagtaataaatggatctcggtaccaaattccagaaaagagacgctttcgagcgtcttttttcgttttggtccgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgccagggtggtttttcttttcaccagtgagacgggcaacagctgattgcccttcaccgcctggccctgagagagttgcagcaagcggtccacgctggtttgccccagcaggcgaaaatcctgtttgatggtggttaacggcgggatataacatgagctgtcttcggtatcgtcgtatcccactaccgagatgtccgcaccaacgcgcagcccggactcggtaatggcgcgcattgcgcccagcgccatctgatcgttggcaaccagcatcgcagtgggaacgatgccctcattcagcatttgcatggtttgttgaaaaccggacatggcactccagtcgccttcccgttccgctatcggctgaatttgattgcgagtgagatatttatgccagccagccagacgcagacgcgccgagacagaacttaatgggcccgctaacagcgcgatttgctggtgacccaatgcgaccagatgctccacgcccagtcgcgtaccgtcttcatgggagaaaataatactgttgatgggtgtctggtcagagacatcaagaaataacgccggaacattagtgcaggcagcttccacagcaatggcatcctggtcatccagcggatagttaatgatcagcccactgacgcgttgcgcgagaagattgtgcaccgccgctttacaggcttcgacgccgcttcgttctaccatcgacaccaccacgctggcacccagttgatcggcgcgagatttaatcgccgcgacaatttgcgacggcgcgtgcagggccagactggaggtggcaacgccaatcagcaacgactgtttgcccgccagttgttgtgccacgcggttgggaatgtaattcagctccgccatcgccgcttccactttttcccgcgttttcgcagaaacgtggctggcctggttcaccacgcgggaaacggtctgataagagacaccggcatactctgcgacatcgtataacgttactggtttcacattcaccaccctgaattgactctcttccgggcgctatcatgccataccgcgaaaggttttgcgccattcgatggtgtcggatcgagatcgatctcgatcccgcgaaattaatacgactcactataggggaattgtgagcggataacaattcctactagagattaaagaggagaaaccctcgatgaagaaaagaggggcgtttttagggctgttgttggtttctgcctgcgcatcagttttcgctgcgaataacgaaacgagcaagagcgtcaccttcccgaagtgcgaggatctggacgcagcgggcattgctg…
encoding: naseq.html

https://synbiohub.org/public/SEGA/SEGA073_seq/1
type: Sequence
persistent identity: SEGA073 seq
display id: SEGA073_seq
version: 1
owned by: carbay
owned by: morno
elements: gtgcatggtctgcaggcgctgccgagccgtattgagcagatgctgtctcaggacaaacgcattgaagcgctggcagaagatttctctgacaaacatcacgcgctgttcctgggccgtggcgatcagtacccaatcgcgctggaaggcgcattgaagttgaaagagatctcttacattcacgctgaagcctacgctgctggcgaactgaaacacggtccgctggcgctaattgatgccgatatgccggttattgttgttgcaccgaacaacgaattgctggaaaaactgaaatccaacattgaagaagttcgcgcgcgtggcggtcagttgtatgtcttcgccgatcaggatgcgggttttgtaagtagcgataacatgcacatcatcgagatgccgcatgtggaagaggtgattgcaccgatcttctacaccgttccgctgcagctgctggcttaccatgtcgcgctgatcaaaggcaccgacgttgaccagccgcgtaacctggcaaaatcggttacggttgagtaataaatggatctcggtaccaaattccagaaaagagacgctttcgagcgtcttttttcgttttggtccgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgccagggtggtttttcttttcaccagtgagacgggcaacagctgattgcccttcaccgcctggccctgagagagttgcagcaagcggtccacgctggtttgccccagcaggcgaaaatcctgtttgatggtggttaacggcgggatataacatgagctgtcttcggtatcgtcgtatcccactaccgagatgtccgcaccaacgcgcagcccggactcggtaatggcgcgcattgcgcccagcgccatctgatcgttggcaaccagcatcgcagtgggaacgatgccctcattcagcatttgcatggtttgttgaaaaccggacatggcactccagtcgccttcccgttccgctatcggctgaatttgattgcgagtgagatatttatgccagccagccagacgcagacgcgccgagacagaacttaatgggcccgctaacagcgcgatttgctggtgacccaatgcgaccagatgctccacgcccagtcgcgtaccgtcttcatgggagaaaataatactgttgatgggtgtctggtcagagacatcaagaaataacgccggaacattagtgcaggcagcttccacagcaatggcatcctggtcatccagcggatagttaatgatcagcccactgacgcgttgcgcgagaagattgtgcaccgccgctttacaggcttcgacgccgcttcgttctaccatcgacaccaccacgctggcacccagttgatcggcgcgagatttaatcgccgcgacaatttgcgacggcgcgtgcagggccagactggaggtggcaacgccaatcagcaacgactgtttgcccgccagttgttgtgccacgcggttgggaatgtaattcagctccgccatcgccgcttccactttttcccgcgttttcgcagaaacgtggctggcctggttcaccacgcgggaaacggtctgataagagacaccggcatactctgcgacatcgtataacgttactggtttcacattcaccaccctgaattgactctcttccgggcgctatcatgccataccgcgaaaggttttgcgccattcgatggtgtcggatcgagatcgatctcgatcccgcgaaattaatacgactcactataggggaattgtgagcggataacaattcctactagagattaaagaggagaaaggggctatgaaaaagagaggggcgtttttagggctgttgttggtttctgcctgcgcatcagttttcgctgcgaataacgaaacgagcaagagcgtcaccttcccgaagtgcgaggatctggacgcagcgggcattgctg…
encoding: naseq.html

https://synbiohub.org/public/SEGA/SEGA098_seq/1
type: Sequence
persistent identity: SEGA098 seq
display id: SEGA098_seq
version: 1
owned by: carbay
owned by: morno
elements: gtgcatggtctgcaggcgctgccgagccgtattgagcagatgctgtctcaggacaaacgcattgaagcgctggcagaagatttctctgacaaacatcacgcgctgttcctgggccgtggcgatcagtacccaatcgcgctggaaggcgcattgaagttgaaagagatctcttacattcacgctgaagcctacgctgctggcgaactgaaacacggtccgctggcgctaattgatgccgatatgccggttattgttgttgcaccgaacaacgaattgctggaaaaactgaaatccaacattgaagaagttcgcgcgcgtggcggtcagttgtatgtcttcgccgatcaggatgcgggttttgtaagtagcgataacatgcacatcatcgagatgccgcatgtggaagaggtgattgcaccgatcttctacaccgttccgctgcagctgctggcttaccatgtcgcgctgatcaaaggcaccgacgttgaccagccgcgtaacctggcaaaatcggttacggttgagtaataaatggatctcggtaccaaattccagaaaagagacgctttcgagcgtcttttttcgttttggtccgcgttgcgcttgacggctagctcagtcctaggtacagtgctagctactagagattaaagaggagaaactatatatgaaagcaattttcgtactgaaacatcttaatcatgctaaggaggttttctaatgagcaaaggagaagaacttttcactggagttgtgccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgctacatacggaaaactcacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactctgacctatggcgttcaatgcttttcccgttatccggatcacatgaaacggcatgactttttcaagagtgccatgcccgaaggttatgtacaggaacgcactatatctttcaaagatgacgggaactacaagacgcgtgctgaagtcaagtttgaaggtgatacccttgttaatcgtatcgagttaaagggtattgattttaaagaagatggaaacattctcggacacaaactagagtacaactataactcacacaatgtatacatcacggcagacaaacaaaagaatggaatcaaagctaacttcaaaattcgccacaacattgaagatggttccgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtcgacacaatctgccctttcgaaagatcccaacgaaaagcgtgaccacatggtccttcttgagtttgtaactgctgctgggattacacatggcatggatgagctctacaaatgataagtactgccgggcctcttgcgggatatcgtccattccgacagcatcgccagtcactatggcgtgctgctagcgctatatgcgttgatgcaatttctatgcgcacccgttctcggagcactgtccgaccgctttggccgccgcccagtcctgctcgcttcgctacttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggattctctacgccggacgcatcgtggccggcatcaccggcgccacaggtgcggttgctggcgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggcgccatctccctgcacgcaccattccttgcggcggcggtgctcaacggcctcaacctactactgggctgcttcctaatgcaggagtcgcataagggagagcgccgtccgatgcccttgagagccttcaacccagtcagctccttccggtgggcgcggggcatgaccattgtggc…
encoding: naseq.html

https://synbiohub.org/public/SEGA/SEGA019_seq/1
type: Sequence
persistent identity: SEGA019 seq
display id: SEGA019_seq
version: 1
owned by: carbay
owned by: morno
elements: gtgcatggtctgcaggcgctgccgagccgtattgagcagatgctgtctcaggacaaacgcattgaagcgctggcagaagatttctctgacaaacatcacgcgctgttcctgggccgtggcgatcagtacccaatcgcgctggaaggcgcattgaagttgaaagagatctcttacattcacgctgaagcctacgctgctggcgaactgaaacacggtccgctggcgctaattgatgccgatatgccggttattgttgttgcaccgaacaacgaattgctggaaaaactgaaatccaacattgaagaagttcgcgcgcgtggcggtcagttgtatgtcttcgccgatcaggatgcgggttttgtaagtagcgataacatgcacatcatcgagatgccgcatgtggaagaggtgattgcaccgatcttctacaccgttccgctgcagctgctggcttaccatgtcgcgctgatcaaaggcaccgacgttgaccagccgcgtaacctggcaaaatcggttacggttgagtaataaatggatctcggtaccaaattccagaaaagagacgctttcgagcgtcttttttcgttttggtccgcgttgcgcttgacggctagctcagtcctaggtacagtgctagctactagagattaaagaggagaaattaattatgaaatatctgctgccgaccgctgctgctggtctgctgctcctcgctgcccagccggcgatggcccgtaaaggcgaagagctgttcactggtgtcgtccctattctggtggaactggatggtgatgtcaacggtcataagttttccgtgcgtggcgagggtgaaggtgacgcaactaatggtaaactgacgctgaagttcatctgtactactggtaaactgccggtaccttggccgactctggtaacgacgctgacttatggtgttcagtgctttgctcgttatccggaccacatgaagcagcatgacttcttcaagtccgccatgccggaaggctatgtgcaggaacgcacgatttcctttaaggatgacggcacgtacaaaacgcgtgcggaagtgaaatttgaaggcgataccctggtaaaccgcattgagctgaaaggcattgactttaaagaagacggcaatatcctgggccataagctggaatacaattttaacagccacaatgtttacatcaccgccgataaacaaaaaaatggcattaaagcgaattttaaaattcgccacaacgtggaggatggcagcgtgcagctggctgatcactaccagcaaaacactccaatcggtgatggtcctgttctgctgccagacaatcactatctgagcacgcaaagcgttctgtctaaagatccgaacgagaaacgcgatcacatggttctgctggagttcgtaaccgcagcgggcatcacgcatggtatggatgaactgtacaaatgaccaattattgaaggccgctaacgcggcctttttttgtttctggtctcccaaaaatttatttgcttattaatcatccggctcgtataatgtgtggagggcccaagttcacttaaaaaggagaatagttatgaaatcgaacaatgcgctcatcgtcatcctcggcaccgtcaccctggatgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagcatcgccagtcactatggcgtgctgctagcgctatatgcgttgatgcaatttctatgcgcacccgttctcggagcactgtccgaccgctttggccgccgcccagtcctgctcgcttcgctacttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggattctctacgccggacgcatcgtggccggcatcaccggcgccacaggtgcggttgctggcgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtg…
encoding: naseq.html

https://synbiohub.org/public/SEGA/SEGA059_seq/1
type: Sequence
persistent identity: SEGA059 seq
display id: SEGA059_seq
version: 1
owned by: carbay
owned by: morno
elements: gtgcatggtctgcaggcgctgccgagccgtattgagcagatgctgtctcaggacaaacgcattgaagcgctggcagaagatttctctgacaaacatcacgcgctgttcctgggccgtggcgatcagtacccaatcgcgctggaaggcgcattgaagttgaaagagatctcttacattcacgctgaagcctacgctgctggcgaactgaaacacggtccgctggcgctaattgatgccgatatgccggttattgttgttgcaccgaacaacgaattgctggaaaaactgaaatccaacattgaagaagttcgcgcgcgtggcggtcagttgtatgtcttcgccgatcaggatgcgggttttgtaagtagcgataacatgcacatcatcgagatgccgcatgtggaagaggtgattgcaccgatcttctacaccgttccgctgcagctgctggcttaccatgtcgcgctgatcaaaggcaccgacgttgaccagccgcgtaacctggcaaaatcggttacggttgagtaataaatggatctcggtaccaaattccagaaaagagacgctttcgagcgtcttttttcgttttggtccgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgccagggtggtttttcttttcaccagtgagacgggcaacagctgattgcccttcaccgcctggccctgagagagttgcagcaagcggtccacgctggtttgccccagcaggcgaaaatcctgtttgatggtggttaacggcgggatataacatgagctgtcttcggtatcgtcgtatcccactaccgagatgtccgcaccaacgcgcagcccggactcggtaatggcgcgcattgcgcccagcgccatctgatcgttggcaaccagcatcgcagtgggaacgatgccctcattcagcatttgcatggtttgttgaaaaccggacatggcactccagtcgccttcccgttccgctatcggctgaatttgattgcgagtgagatatttatgccagccagccagacgcagacgcgccgagacagaacttaatgggcccgctaacagcgcgatttgctggtgacccaatgcgaccagatgctccacgcccagtcgcgtaccgtcttcatgggagaaaataatactgttgatgggtgtctggtcagagacatcaagaaataacgccggaacattagtgcaggcagcttccacagcaatggcatcctggtcatccagcggatagttaatgatcagcccactgacgcgttgcgcgagaagattgtgcaccgccgctttacaggcttcgacgccgcttcgttctaccatcgacaccaccacgctggcacccagttgatcggcgcgagatttaatcgccgcgacaatttgcgacggcgcgtgcagggccagactggaggtggcaacgccaatcagcaacgactgtttgcccgccagttgttgtgccacgcggttgggaatgtaattcagctccgccatcgccgcttccactttttcccgcgttttcgcagaaacgtggctggcctggttcaccacgcgggaaacggtctgataagagacaccggcatactctgcgacatcgtataacgttactggtttcacattcaccaccctgaattgactctcttccgggcgctatcatgccataccgcgaaaggttttgcgccattcgatggtgtcggatcgagatcgatctcgatcccgcgaaattaatacgactcactataggggaattgtgagcggataacaattcctactagagattaaagaggagaaaggggggatgcaaattttcgtgaaaacactgaccggcaaaaccattaccctggaggtggagcctggcagcgcccatcatcatcaccatcaccatcaccatcatgctggtagcagcgatacgatcgaaaacgtcaaagcca…
encoding: naseq.html

https://synbiohub.org/public/SEGA/SEGA055_seq/1
type: Sequence
persistent identity: SEGA055 seq
display id: SEGA055_seq
version: 1
owned by: carbay
owned by: morno
elements: gtgcatggtctgcaggcgctgccgagccgtattgagcagatgctgtctcaggacaaacgcattgaagcgctggcagaagatttctctgacaaacatcacgcgctgttcctgggccgtggcgatcagtacccaatcgcgctggaaggcgcattgaagttgaaagagatctcttacattcacgctgaagcctacgctgctggcgaactgaaacacggtccgctggcgctaattgatgccgatatgccggttattgttgttgcaccgaacaacgaattgctggaaaaactgaaatccaacattgaagaagttcgcgcgcgtggcggtcagttgtatgtcttcgccgatcaggatgcgggttttgtaagtagcgataacatgcacatcatcgagatgccgcatgtggaagaggtgattgcaccgatcttctacaccgttccgctgcagctgctggcttaccatgtcgcgctgatcaaaggcaccgacgttgaccagccgcgtaacctggcaaaatcggttacggttgagtaataaatggatctcggtaccaaattccagaaaagagacgctttcgagcgtcttttttcgttttggtccgcgttgcgcttaatctttctgcgaattgagatgacgccactggctgggcgtcatcccggtttcccgggtaaacaccaccgaaaaatagttactatcttcaaagccacattcggtcgaaatatcactgattaacaggcggctatgctggagaagatattgcgcatgacacactctgacctgtcgcagatattgattgatggtcattccagtctgctggcgaaattgctgacgcaaaacgcgctcactgcacgatgcctcatcacaaaatttatccagcgcaaagggacttttcaggctagccgccagccgggtaatcagcttatccagcaacgtttcgctggatgttggcggcaacgaatcactggtgtaacgatggcgattcagcaacatcaccaactgcccgaacagcaactcagccatttcgttagcaaacggcacatgctgactactttcatgctcaagctgaccaataacctgccgcgcctgcgccatccccatgctacctaagcgccagtgtggttgccctgcgctggcgttaaatcccggaatcgccccctgccagtcaagattcagcttcagacgctccgggcaataaataatattctgcaaaaccagatcgttaacggaagcgtaggagtgtttatcatcagcatgaatgtaaaagagatcgccacgggtaatgcgataagggcgatcgttgagtacatgcaggccattaccgcgccagacaatcaccagctcacaaaaatcatgtgtatgttcagcaaagacatcttgcggataacggtcagccacagcgactgcctgctggtcgctggcaaaaaaatcatctttgagaagttttaactgatgcgccaccgtggctacctcggccagagaacgaagttgattattcgcaatatggcgtacaaatacgttgagaagattcgcgttattgcagaaagccatcccgtccctggcgaatatcacgcggtgaccagttaaactctcggcgaaaaagcgtcgaaaagtggttactgtcgctgaatccacagcgataggcgatgtcagtaacgctggcctcgctgtggcgtagcagatgtcgggctttcatcagtcgcaggcggttcaggtatcgctgaggcgtcagtcccgtttgctgcttaagctgccgatgtagcgtacgcagtgaaagagaaaattgatccgccacggcatcccaattcacctcatcggcaaaatggtcctccagccaggccagaagcaagttgagacgtgatgcgctgttttccaggttctcctgcaaactgcttttacgcagcaagagcagtaattgcataaacaagatctcgcgactggcggtcgagggtaaatcattttccccttcctgctgttccatctgtgcaaccagctgtcgcacctgctgc…
encoding: naseq.html

https://synbiohub.org/public/SEGA/SEGA095_seq/1
type: Sequence
persistent identity: SEGA095 seq
display id: SEGA095_seq
version: 1
owned by: carbay
owned by: morno
elements: gtgcatggtctgcaggcgctgccgagccgtattgagcagatgctgtctcaggacaaacgcattgaagcgctggcagaagatttctctgacaaacatcacgcgctgttcctgggccgtggcgatcagtacccaatcgcgctggaaggcgcattgaagttgaaagagatctcttacattcacgctgaagcctacgctgctggcgaactgaaacacggtccgctggcgctaattgatgccgatatgccggttattgttgttgcaccgaacaacgaattgctggaaaaactgaaatccaacattgaagaagttcgcgcgcgtggcggtcagttgtatgtcttcgccgatcaggatgcgggttttgtaagtagcgataacatgcacatcatcgagatgccgcatgtggaagaggtgattgcaccgatcttctacaccgttccgctgcagctgctggcttaccatgtcgcgctgatcaaaggcaccgacgttgaccagccgcgtaacctggcaaaatcggttacggttgagtaataaatggatctcggtaccaaattccagaaaagagacgctttcgagcgtcttttttcgttttggtccaccgtattcatcagcactgtcctgctccttgtgatggtttacaaacgtaaaaagtctctttaatacctgtttttgcttcatattgttcagcgacagcttgctgtacggcaggcaccagctcttccgggatcagcgcgacgatacagccgccaaatccgccgccggtcatgcgtacgccacctttgtcgccaatcacagctttgacgatttctaccagagtgtcaatttgcggcacggtgatttcgaaatcatcgcgcatagaggcatgagactccgccatcaactcgcccatacgtttcaggtcgccttgctccagcgcgctggcagcttcaacggtgcgggcgttttcagtcagtatatgacgcacgcgttttgccacgatcgggtccagttcatgcgcaacagcgttgaactcttcaatggtgacatcacgcagggctggctgctggaagaaacgcgcaccggtttcgcactgttcacgacgggtgttgtattcgctgccaaccagggtacgtttgaagttactgttgatgatgacgacagccacacctttgggcatggaaactgctttggtccccagtgagcggcaatcgatcagcaaggcatgatctttcttgccgagcgcggaaattagctgatccatgatcccgcagttacagcctacaaactggttttctgcttcctgaccgttaagcgcgatttgtgcgccgtccagcggcagatgataaagctgctgcaatacggttccgaccgcgacttccagtgaagcggaagaacttaacccggcaccctgcggcacattgccgctgatcaccatgtccacgccgccgaagctgttgttacgcagttgcagatgtttcaccacgccacgaacgtagttagcccattgatagttttcatgtgcgacaatgggcgcatcgagggaaaactcgtcgagctgattttcataatcggctgccatcacgcgaactttacggtcatcgcgtggtgcacaactgatcacggtttgataatcaatcgcgcagggcagaacgaaaccgtcgttgtagtcggtgtgttcaccaatcaaattcacgcggccaggcgcctgaatggtgtgagtggcagggtagccaaatgcgttggcaaacagagattgtgttttttctttcagactcatgattgcctcctgggttagttcctcaccttgtcgtattatactatgccgatatactatgccgatgattaattgtcaacctttaatgaattataaaacgaaaggctcagtcgaaagactgggcctttcgttttatgcgttgcgcttaatctttctgcgaattgagatgacgccactggctgggcgtcatcccggtttcccgggtaaacaccaccgaaaaatagttactatcttcaaagccacattcggt…
encoding: naseq.html

https://synbiohub.org/public/SEGA/SEGA030_seq/1
type: Sequence
persistent identity: SEGA030 seq
display id: SEGA030_seq
version: 1
owned by: carbay
owned by: morno
elements: gtgcatggtctgcaggcgctgccgagccgtattgagcagatgctgtctcaggacaaacgcattgaagcgctggcagaagatttctctgacaaacatcacgcgctgttcctgggccgtggcgatcagtacccaatcgcgctggaaggcgcattgaagttgaaagagatctcttacattcacgctgaagcctacgctgctggcgaactgaaacacggtccgctggcgctaattgatgccgatatgccggttattgttgttgcaccgaacaacgaattgctggaaaaactgaaatccaacattgaagaagttcgcgcgcgtggcggtcagttgtatgtcttcgccgatcaggatgcgggttttgtaagtagcgataacatgcacatcatcgagatgccgcatgtggaagaggtgattgcaccgatcttctacaccgttccgctgcagctgctggcttaccatgtcgcgctgatcaaaggcaccgacgttgaccagccgcgtaacctggcaaaatcggttacggttgagtaataaatggatctcggtaccaaattccagaaaagagacgctttcgagcgtcttttttcgttttggtccgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgccagggtggtttttcttttcaccagtgagacgggcaacagctgattgcccttcaccgcctggccctgagagagttgcagcaagcggtccacgctggtttgccccagcaggcgaaaatcctgtttgatggtggttaacggcgggatataacatgagctgtcttcggtatcgtcgtatcccactaccgagatgtccgcaccaacgcgcagcccggactcggtaatggcgcgcattgcgcccagcgccatctgatcgttggcaaccagcatcgcagtgggaacgatgccctcattcagcatttgcatggtttgttgaaaaccggacatggcactccagtcgccttcccgttccgctatcggctgaatttgattgcgagtgagatatttatgccagccagccagacgcagacgcgccgagacagaacttaatgggcccgctaacagcgcgatttgctggtgacccaatgcgaccagatgctccacgcccagtcgcgtaccgtcttcatgggagaaaataatactgttgatgggtgtctggtcagagacatcaagaaataacgccggaacattagtgcaggcagcttccacagcaatggcatcctggtcatccagcggatagttaatgatcagcccactgacgcgttgcgcgagaagattgtgcaccgccgctttacaggcttcgacgccgcttcgttctaccatcgacaccaccacgctggcacccagttgatcggcgcgagatttaatcgccgcgacaatttgcgacggcgcgtgcagggccagactggaggtggcaacgccaatcagcaacgactgtttgcccgccagttgttgtgccacgcggttgggaatgtaattcagctccgccatcgccgcttccactttttcccgcgttttcgcagaaacgtggctggcctggttcaccacgcgggaaacggtctgataagagacaccggcatactctgcgacatcgtataacgttactggtttcacattcaccaccctgaattgactctcttccgggcgctatcatgccataccgcgaaaggttttgcgccattcgatggtgtcggatcgagatcgatctcgatcccgcgaaatttgacaattaatcatccggctcgtataatggaattgtgagcggataacaattcctactagagattaaagaggagaaaaactacatggaaaacctgtacttccagtttcgtagcaaaggagaagaacttgtcactggagttagcaaaggagaagaacttttcactggagttgtgccaattcttgttgaattagatggtgatgttaat…
encoding: naseq.html

https://synbiohub.org/public/SEGA/SEGA020_seq/1
type: Sequence
persistent identity: SEGA020 seq
display id: SEGA020_seq
version: 1
owned by: carbay
owned by: morno
elements: gtgcatggtctgcaggcgctgccgagccgtattgagcagatgctgtctcaggacaaacgcattgaagcgctggcagaagatttctctgacaaacatcacgcgctgttcctgggccgtggcgatcagtacccaatcgcgctggaaggcgcattgaagttgaaagagatctcttacattcacgctgaagcctacgctgctggcgaactgaaacacggtccgctggcgctaattgatgccgatatgccggttattgttgttgcaccgaacaacgaattgctggaaaaactgaaatccaacattgaagaagttcgcgcgcgtggcggtcagttgtatgtcttcgccgatcaggatgcgggttttgtaagtagcgataacatgcacatcatcgagatgccgcatgtggaagaggtgattgcaccgatcttctacaccgttccgctgcagctgctggcttaccatgtcgcgctgatcaaaggcaccgacgttgaccagccgcgtaacctggcaaaatcggttacggttgagtaataaatggatctcggtaccaaattccagaaaagagacgctttcgagcgtcttttttcgttttggtccgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgccagggtggtttttcttttcaccagtgagacgggcaacagctgattgcccttcaccgcctggccctgagagagttgcagcaagcggtccacgctggtttgccccagcaggcgaaaatcctgtttgatggtggttaacggcgggatataacatgagctgtcttcggtatcgtcgtatcccactaccgagatgtccgcaccaacgcgcagcccggactcggtaatggcgcgcattgcgcccagcgccatctgatcgttggcaaccagcatcgcagtgggaacgatgccctcattcagcatttgcatggtttgttgaaaaccggacatggcactccagtcgccttcccgttccgctatcggctgaatttgattgcgagtgagatatttatgccagccagccagacgcagacgcgccgagacagaacttaatgggcccgctaacagcgcgatttgctggtgacccaatgcgaccagatgctccacgcccagtcgcgtaccgtcttcatgggagaaaataatactgttgatgggtgtctggtcagagacatcaagaaataacgccggaacattagtgcaggcagcttccacagcaatggcatcctggtcatccagcggatagttaatgatcagcccactgacgcgttgcgcgagaagattgtgcaccgccgctttacaggcttcgacgccgcttcgttctaccatcgacaccaccacgctggcacccagttgatcggcgcgagatttaatcgccgcgacaatttgcgacggcgcgtgcagggccagactggaggtggcaacgccaatcagcaacgactgtttgcccgccagttgttgtgccacgcggttgggaatgtaattcagctccgccatcgccgcttccactttttcccgcgttttcgcagaaacgtggctggcctggttcaccacgcgggaaacggtctgataagagacaccggcatactctgcgacatcgtataacgttactggtttcacattcaccaccctgaattgactctcttccgggcgctatcatgccataccgcgaaaggttttgcgccattcgatggtgtcggatcgagatcgatctcgatcccgcgaaatttgacaattaatcatccggctcgtataatggaattgtgagcggataacaattcctactagagattaaagaggagaaaggggggatgaaggctattttcgtactgaaacatcttaatcatgctaaggaggttttctaatgagcaaaggagaagaacttttcactggagttgtgccaattcttgttgaattagatggtgatgttaatg…
encoding: naseq.html

https://synbiohub.org/public/SEGA/SEGA101_seq/1
type: Sequence
persistent identity: SEGA101 seq
display id: SEGA101_seq
version: 1
owned by: carbay
owned by: morno
elements: gtgcatggtctgcaggcgctgccgagccgtattgagcagatgctgtctcaggacaaacgcattgaagcgctggcagaagatttctctgacaaacatcacgcgctgttcctgggccgtggcgatcagtacccaatcgcgctggaaggcgcattgaagttgaaagagatctcttacattcacgctgaagcctacgctgctggcgaactgaaacacggtccgctggcgctaattgatgccgatatgccggttattgttgttgcaccgaacaacgaattgctggaaaaactgaaatccaacattgaagaagttcgcgcgcgtggcggtcagttgtatgtcttcgccgatcaggatgcgggttttgtaagtagcgataacatgcacatcatcgagatgccgcatgtggaagaggtgattgcaccgatcttctacaccgttccgctgcagctgctggcttaccatgtcgcgctgatcaaaggcaccgacgttgaccagccgcgtaacctggcaaaatcggttacggttgagtaataaatggatctcggtaccaaattccagaaaagagacgctttcgagcgtcttttttcgttttggtccgcgttgcgcttaatctttctgcgaattgagatgacgccactggctgggcgtcatcccggtttcccgggtaaacaccaccgaaaaatagttactatcttcaaagccacattcggtcgaaatatcactgattaacaggcggctatgctggagaagatattgcgcatgacacactctgacctgtcgcagatattgattgatggtcattccagtctgctggcgaaattgctgacgcaaaacgcgctcactgcacgatgcctcatcacaaaatttatccagcgcaaagggacttttcaggctagccgccagccgggtaatcagcttatccagcaacgtttcgctggatgttggcggcaacgaatcactggtgtaacgatggcgattcagcaacatcaccaactgcccgaacagcaactcagccatttcgttagcaaacggcacatgctgactactttcatgctcaagctgaccaataacctgccgcgcctgcgccatccccatgctacctaagcgccagtgtggttgccctgcgctggcgttaaatcccggaatcgccccctgccagtcaagattcagcttcagacgctccgggcaataaataatattctgcaaaaccagatcgttaacggaagcgtaggagtgtttatcatcagcatgaatgtaaaagagatcgccacgggtaatgcgataagggcgatcgttgagtacatgcaggccattaccgcgccagacaatcaccagctcacaaaaatcatgtgtatgttcagcaaagacatcttgcggataacggtcagccacagcgactgcctgctggtcgctggcaaaaaaatcatctttgagaagttttaactgatgcgccaccgtggctacctcggccagagaacgaagttgattattcgcaatatggcgtacaaatacgttgagaagattcgcgttattgcagaaagccatcccgtccctggcgaatatcacgcggtgaccagttaaactctcggcgaaaaagcgtcgaaaagtggttactgtcgctgaatccacagcgataggcgatgtcagtaacgctggcctcgctgtggcgtagcagatgtcgggctttcatcagtcgcaggcggttcaggtatcgctgaggcgtcagtcccgtttgctgcttaagctgccgatgtagcgtacgcagtgaaagagaaaattgatccgccacggcatcccaattcacctcatcggcaaaatggtcctccagccaggccagaagcaagttgagacgtgatgcgctgttttccaggttctcctgcaaactgcttttacgcagcaagagcagtaattgcataaacaagatctcgcgactggcggtcgagggtaaatcattttccccttcctgctgttccatctgtgcaaccagctgtcgcacctgctgc…
encoding: naseq.html

https://synbiohub.org/public/SEGA/SEGA053_seq/1
type: Sequence
persistent identity: SEGA053 seq
display id: SEGA053_seq
version: 1
owned by: carbay
owned by: morno
elements: gtgcatggtctgcaggcgctgccgagccgtattgagcagatgctgtctcaggacaaacgcattgaagcgctggcagaagatttctctgacaaacatcacgcgctgttcctgggccgtggcgatcagtacccaatcgcgctggaaggcgcattgaagttgaaagagatctcttacattcacgctgaagcctacgctgctggcgaactgaaacacggtccgctggcgctaattgatgccgatatgccggttattgttgttgcaccgaacaacgaattgctggaaaaactgaaatccaacattgaagaagttcgcgcgcgtggcggtcagttgtatgtcttcgccgatcaggatgcgggttttgtaagtagcgataacatgcacatcatcgagatgccgcatgtggaagaggtgattgcaccgatcttctacaccgttccgctgcagctgctggcttaccatgtcgcgctgatcaaaggcaccgacgttgaccagccgcgtaacctggcaaaatcggttacggttgagtaataaatggatctcggtaccaaattccagaaaagagacgctttcgagcgtcttttttcgttttggtccgcgttgcgcttaatctttctgcgaattgagatgacgccactggctgggcgtcatcccggtttcccgggtaaacaccaccgaaaaatagttactatcttcaaagccacattcggtcgaaatatcactgattaacaggcggctatgctggagaagatattgcgcatgacacactctgacctgtcgcagatattgattgatggtcattccagtctgctggcgaaattgctgacgcaaaacgcgctcactgcacgatgcctcatcacaaaatttatccagcgcaaagggacttttcaggctagccgccagccgggtaatcagcttatccagcaacgtttcgctggatgttggcggcaacgaatcactggtgtaacgatggcgattcagcaacatcaccaactgcccgaacagcaactcagccatttcgttagcaaacggcacatgctgactactttcatgctcaagctgaccaataacctgccgcgcctgcgccatccccatgctacctaagcgccagtgtggttgccctgcgctggcgttaaatcccggaatcgccccctgccagtcaagattcagcttcagacgctccgggcaataaataatattctgcaaaaccagatcgttaacggaagcgtaggagtgtttatcatcagcatgaatgtaaaagagatcgccacgggtaatgcgataagggcgatcgttgagtacatgcaggccattaccgcgccagacaatcaccagctcacaaaaatcatgtgtatgttcagcaaagacatcttgcggataacggtcagccacagcgactgcctgctggtcgctggcaaaaaaatcatctttgagaagttttaactgatgcgccaccgtggctacctcggccagagaacgaagttgattattcgcaatatggcgtacaaatacgttgagaagattcgcgttattgcagaaagccatcccgtccctggcgaatatcacgcggtgaccagttaaactctcggcgaaaaagcgtcgaaaagtggttactgtcgctgaatccacagcgataggcgatgtcagtaacgctggcctcgctgtggcgtagcagatgtcgggctttcatcagtcgcaggcggttcaggtatcgctgaggcgtcagtcccgtttgctgcttaagctgccgatgtagcgtacgcagtgaaagagaaaattgatccgccacggcatcccaattcacctcatcggcaaaatggtcctccagccaggccagaagcaagttgagacgtgatgcgctgttttccaggttctcctgcaaactgcttttacgcagcaagagcagtaattgcataaacaagatctcgcgactggcggtcgagggtaaatcattttccccttcctgctgttccatctgtgcaaccagctgtcgcacctgctgc…
encoding: naseq.html

https://synbiohub.org/public/SEGA/SEGA102_seq/1
type: Sequence
persistent identity: SEGA102 seq
display id: SEGA102_seq
version: 1
owned by: carbay
owned by: morno
elements: gtgcatggtctgcaggcgctgccgagccgtattgagcagatgctgtctcaggacaaacgcattgaagcgctggcagaagatttctctgacaaacatcacgcgctgttcctgggccgtggcgatcagtacccaatcgcgctggaaggcgcattgaagttgaaagagatctcttacattcacgctgaagcctacgctgctggcgaactgaaacacggtccgctggcgctaattgatgccgatatgccggttattgttgttgcaccgaacaacgaattgctggaaaaactgaaatccaacattgaagaagttcgcgcgcgtggcggtcagttgtatgtcttcgccgatcaggatgcgggttttgtaagtagcgataacatgcacatcatcgagatgccgcatgtggaagaggtgattgcaccgatcttctacaccgttccgctgcagctgctggcttaccatgtcgcgctgatcaaaggcaccgacgttgaccagccgcgtaacctggcaaaatcggttacggttgagtaataaatggatctcggtaccaaattccagaaaagagacgctttcgagcgtcttttttcgttttggtccgcgttgcgcttaatctttctgcgaattgagatgacgccactggctgggcgtcatcccggtttcccgggtaaacaccaccgaaaaatagttactatcttcaaagccacattcggtcgaaatatcactgattaacaggcggctatgctggagaagatattgcgcatgacacactctgacctgtcgcagatattgattgatggtcattccagtctgctggcgaaattgctgacgcaaaacgcgctcactgcacgatgcctcatcacaaaatttatccagcgcaaagggacttttcaggctagccgccagccgggtaatcagcttatccagcaacgtttcgctggatgttggcggcaacgaatcactggtgtaacgatggcgattcagcaacatcaccaactgcccgaacagcaactcagccatttcgttagcaaacggcacatgctgactactttcatgctcaagctgaccaataacctgccgcgcctgcgccatccccatgctacctaagcgccagtgtggttgccctgcgctggcgttaaatcccggaatcgccccctgccagtcaagattcagcttcagacgctccgggcaataaataatattctgcaaaaccagatcgttaacggaagcgtaggagtgtttatcatcagcatgaatgtaaaagagatcgccacgggtaatgcgataagggcgatcgttgagtacatgcaggccattaccgcgccagacaatcaccagctcacaaaaatcatgtgtatgttcagcaaagacatcttgcggataacggtcagccacagcgactgcctgctggtcgctggcaaaaaaatcatctttgagaagttttaactgatgcgccaccgtggctacctcggccagagaacgaagttgattattcgcaatatggcgtacaaatacgttgagaagattcgcgttattgcagaaagccatcccgtccctggcgaatatcacgcggtgaccagttaaactctcggcgaaaaagcgtcgaaaagtggttactgtcgctgaatccacagcgataggcgatgtcagtaacgctggcctcgctgtggcgtagcagatgtcgggctttcatcagtcgcaggcggttcaggtatcgctgaggcgtcagtcccgtttgctgcttaagctgccgatgtagcgtacgcagtgaaagagaaaattgatccgccacggcatcccaattcacctcatcggcaaaatggtcctccagccaggccagaagcaagttgagacgtgatgcgctgttttccaggttctcctgcaaactgcttttacgcagcaagagcagtaattgcataaacaagatctcgcgactggcggtcgagggtaaatcattttccccttcctgctgttccatctgtgcaaccagctgtcgcacctgctgc…
encoding: naseq.html

https://synbiohub.org/public/SEGA/SEGA041_seq/1
type: Sequence
persistent identity: SEGA041 seq
display id: SEGA041_seq
version: 1
owned by: carbay
owned by: morno
elements: gtgcatggtctgcaggcgctgccgagccgtattgagcagatgctgtctcaggacaaacgcattgaagcgctggcagaagatttctctgacaaacatcacgcgctgttcctgggccgtggcgatcagtacccaatcgcgctggaaggcgcattgaagttgaaagagatctcttacattcacgctgaagcctacgctgctggcgaactgaaacacggtccgctggcgctaattgatgccgatatgccggttattgttgttgcaccgaacaacgaattgctggaaaaactgaaatccaacattgaagaagttcgcgcgcgtggcggtcagttgtatgtcttcgccgatcaggatgcgggttttgtaagtagcgataacatgcacatcatcgagatgccgcatgtggaagaggtgattgcaccgatcttctacaccgttccgctgcagctgctggcttaccatgtcgcgctgatcaaaggcaccgacgttgaccagccgcgtaacctggcaaaatcggttacggttgagtaataaatggatctcggtaccaaattccagaaaagagacgctttcgagcgtcttttttcgttttggtccgcgttgcgcttaatctttctgcgaattgagatgacgccactggctgggcgtcatcccggtttcccgggtaaacaccaccgaaaaatagttactatcttcaaagccacattcggtcgaaatatcactgattaacaggcggctatgctggagaagatattgcgcatgacacactctgacctgtcgcagatattgattgatggtcattccagtctgctggcgaaattgctgacgcaaaacgcgctcactgcacgatgcctcatcacaaaatttatccagcgcaaagggacttttcaggctagccgccagccgggtaatcagcttatccagcaacgtttcgctggatgttggcggcaacgaatcactggtgtaacgatggcgattcagcaacatcaccaactgcccgaacagcaactcagccatttcgttagcaaacggcacatgctgactactttcatgctcaagctgaccaataacctgccgcgcctgcgccatccccatgctacctaagcgccagtgtggttgccctgcgctggcgttaaatcccggaatcgccccctgccagtcaagattcagcttcagacgctccgggcaataaataatattctgcaaaaccagatcgttaacggaagcgtaggagtgtttatcatcagcatgaatgtaaaagagatcgccacgggtaatgcgataagggcgatcgttgagtacatgcaggccattaccgcgccagacaatcaccagctcacaaaaatcatgtgtatgttcagcaaagacatcttgcggataacggtcagccacagcgactgcctgctggtcgctggcaaaaaaatcatctttgagaagttttaactgatgcgccaccgtggctacctcggccagagaacgaagttgattattcgcaatatggcgtacaaatacgttgagaagattcgcgttattgcagaaagccatcccgtccctggcgaatatcacgcggtgaccagttaaactctcggcgaaaaagcgtcgaaaagtggttactgtcgctgaatccacagcgataggcgatgtcagtaacgctggcctcgctgtggcgtagcagatgtcgggctttcatcagtcgcaggcggttcaggtatcgctgaggcgtcagtcccgtttgctgcttaagctgccgatgtagcgtacgcagtgaaagagaaaattgatccgccacggcatcccaattcacctcatcggcaaaatggtcctccagccaggccagaagcaagttgagacgtgatgcgctgttttccaggttctcctgcaaactgcttttacgcagcaagagcagtaattgcataaacaagatctcgcgactggcggtcgagggtaaatcattttccccttcctgctgttccatctgtgcaaccagctgtcgcacctgctgc…
encoding: naseq.html

https://synbiohub.org/public/SEGA/SEGA083_seq/1
type: Sequence
persistent identity: SEGA083 seq
display id: SEGA083_seq
version: 1
owned by: carbay
owned by: morno
elements: gtgcatggtctgcaggcgctgccgagccgtattgagcagatgctgtctcaggacaaacgcattgaagcgctggcagaagatttctctgacaaacatcacgcgctgttcctgggccgtggcgatcagtacccaatcgcgctggaaggcgcattgaagttgaaagagatctcttacattcacgctgaagcctacgctgctggcgaactgaaacacggtccgctggcgctaattgatgccgatatgccggttattgttgttgcaccgaacaacgaattgctggaaaaactgaaatccaacattgaagaagttcgcgcgcgtggcggtcagttgtatgtcttcgccgatcaggatgcgggttttgtaagtagcgataacatgcacatcatcgagatgccgcatgtggaagaggtgattgcaccgatcttctacaccgttccgctgcagctgctggcttaccatgtcgcgctgatcaaaggcaccgacgttgaccagccgcgtaacctggcaaaatcggttacggttgagtaataaatggatctcggtaccaaattccagaaaagagacgctttcgagcgtcttttttcgttttggtccaccgtattcatcagcactgtcctgctccttgtgatggtttacaaacgtaaaaagtctctttaatacctgtttttgcttcatattgttcagcgacagcttgctgtacggcaggcaccagctcttccgggatcagcgcgacgatacagccgccaaatccgccgccggtcatgcgtacgccacctttgtcgccaatcacagctttgacgatttctaccagagtgtcaatttgcggcacggtgatttcgaaatcatcgcgcatagaggcatgagactccgccatcaactcgcccatacgtttcaggtcgccttgctccagcgcgctggcagcttcaacggtgcgggcgttttcagtcagtatatgacgcacgcgttttgccacgatcgggtccagttcatgcgcaacagcgttgaactcttcaatggtgacatcacgcagggctggctgctggaagaaacgcgcaccggtttcgcactgttcacgacgggtgttgtattcgctgccaaccagggtacgtttgaagttactgttgatgatgacgacagccacacctttgggcatggaaactgctttggtccccagtgagcggcaatcgatcagcaaggcatgatctttcttgccgagcgcggaaattagctgatccatgatcccgcagttacagcctacaaactggttttctgcttcctgaccgttaagcgcgatttgtgcgccgtccagcggcagatgataaagctgctgcaatacggttccgaccgcgacttccagtgaagcggaagaacttaacccggcaccctgcggcacattgccgctgatcaccatgtccacgccgccgaagctgttgttacgcagttgcagatgtttcaccacgccacgaacgtagttagcccattgatagttttcatgtgcgacaatgggcgcatcgagggaaaactcgtcgagctgattttcataatcggctgccatcacgcgaactttacggtcatcgcgtggtgcacaactgatcacggtttgataatcaatcgcgcagggcagaacgaaaccgtcgttgtagtcggtgtgttcaccaatcaaattcacgcggccaggcgcctgaatggtgtgagtggcagggtagccaaatgcgttggcaaacagagattgtgttttttctttcagactcatgattgcctcctgggttagttcctcaccttgtcgtattatactatgccgatatactatgccgatgattaattgtcaacctttaatgaattataaaacgaaaggctcagtcgaaagactgggcctttcgttttatgcgttgcgcttaatctttctgcgaattgagatgacgccactggctgggcgtcatcccggtttcccgggtaaacaccaccgaaaaatagttactatcttcaaagccacattcggt…
encoding: naseq.html

https://synbiohub.org/public/SEGA/SEGA065_seq/1
type: Sequence
persistent identity: SEGA065 seq
display id: SEGA065_seq
version: 1
owned by: carbay
owned by: morno
elements: gtgcatggtctgcaggcgctgccgagccgtattgagcagatgctgtctcaggacaaacgcattgaagcgctggcagaagatttctctgacaaacatcacgcgctgttcctgggccgtggcgatcagtacccaatcgcgctggaaggcgcattgaagttgaaagagatctcttacattcacgctgaagcctacgctgctggcgaactgaaacacggtccgctggcgctaattgatgccgatatgccggttattgttgttgcaccgaacaacgaattgctggaaaaactgaaatccaacattgaagaagttcgcgcgcgtggcggtcagttgtatgtcttcgccgatcaggatgcgggttttgtaagtagcgataacatgcacatcatcgagatgccgcatgtggaagaggtgattgcaccgatcttctacaccgttccgctgcagctgctggcttaccatgtcgcgctgatcaaaggcaccgacgttgaccagccgcgtaacctggcaaaatcggttacggttgagtaataaatggatctcggtaccaaattccagaaaagagacgctttcgagcgtcttttttcgttttggtccgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgccagggtggtttttcttttcaccagtgagacgggcaacagctgattgcccttcaccgcctggccctgagagagttgcagcaagcggtccacgctggtttgccccagcaggcgaaaatcctgtttgatggtggttaacggcgggatataacatgagctgtcttcggtatcgtcgtatcccactaccgagatgtccgcaccaacgcgcagcccggactcggtaatggcgcgcattgcgcccagcgccatctgatcgttggcaaccagcatcgcagtgggaacgatgccctcattcagcatttgcatggtttgttgaaaaccggacatggcactccagtcgccttcccgttccgctatcggctgaatttgattgcgagtgagatatttatgccagccagccagacgcagacgcgccgagacagaacttaatgggcccgctaacagcgcgatttgctggtgacccaatgcgaccagatgctccacgcccagtcgcgtaccgtcttcatgggagaaaataatactgttgatgggtgtctggtcagagacatcaagaaataacgccggaacattagtgcaggcagcttccacagcaatggcatcctggtcatccagcggatagttaatgatcagcccactgacgcgttgcgcgagaagattgtgcaccgccgctttacaggcttcgacgccgcttcgttctaccatcgacaccaccacgctggcacccagttgatcggcgcgagatttaatcgccgcgacaatttgcgacggcgcgtgcagggccagactggaggtggcaacgccaatcagcaacgactgtttgcccgccagttgttgtgccacgcggttgggaatgtaattcagctccgccatcgccgcttccactttttcccgcgttttcgcagaaacgtggctggcctggttcaccacgcgggaaacggtctgataagagacaccggcatactctgcgacatcgtataacgttactggtttcacattcaccaccctgaattgactctcttccgggcgctatcatgccataccgcgaaaggttttgcgccattcgatggtgtcggatcgagatcgatctcgatcccgcgaaattaatacgactcactataggggaattgtgagcggataacaattcctactagagattaaagaggagaaacgggggatggagaacctgtacttccagtttcgtagcaaaggagaagaacttgtcactggagttagcaaaggagaagaacttttcactggagttgtgccaattcttgttgaattagatggtgatgttaatgggcacaaat…
encoding: naseq.html

https://synbiohub.org/public/SEGA/SEGA070_seq/1
type: Sequence
persistent identity: SEGA070 seq
display id: SEGA070_seq
version: 1
owned by: carbay
owned by: morno
elements: gtgcatggtctgcaggcgctgccgagccgtattgagcagatgctgtctcaggacaaacgcattgaagcgctggcagaagatttctctgacaaacatcacgcgctgttcctgggccgtggcgatcagtacccaatcgcgctggaaggcgcattgaagttgaaagagatctcttacattcacgctgaagcctacgctgctggcgaactgaaacacggtccgctggcgctaattgatgccgatatgccggttattgttgttgcaccgaacaacgaattgctggaaaaactgaaatccaacattgaagaagttcgcgcgcgtggcggtcagttgtatgtcttcgccgatcaggatgcgggttttgtaagtagcgataacatgcacatcatcgagatgccgcatgtggaagaggtgattgcaccgatcttctacaccgttccgctgcagctgctggcttaccatgtcgcgctgatcaaaggcaccgacgttgaccagccgcgtaacctggcaaaatcggttacggttgagtaataaatggatctcggtaccaaattccagaaaagagacgctttcgagcgtcttttttcgttttggtccgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgccagggtggtttttcttttcaccagtgagacgggcaacagctgattgcccttcaccgcctggccctgagagagttgcagcaagcggtccacgctggtttgccccagcaggcgaaaatcctgtttgatggtggttaacggcgggatataacatgagctgtcttcggtatcgtcgtatcccactaccgagatgtccgcaccaacgcgcagcccggactcggtaatggcgcgcattgcgcccagcgccatctgatcgttggcaaccagcatcgcagtgggaacgatgccctcattcagcatttgcatggtttgttgaaaaccggacatggcactccagtcgccttcccgttccgctatcggctgaatttgattgcgagtgagatatttatgccagccagccagacgcagacgcgccgagacagaacttaatgggcccgctaacagcgcgatttgctggtgacccaatgcgaccagatgctccacgcccagtcgcgtaccgtcttcatgggagaaaataatactgttgatgggtgtctggtcagagacatcaagaaataacgccggaacattagtgcaggcagcttccacagcaatggcatcctggtcatccagcggatagttaatgatcagcccactgacgcgttgcgcgagaagattgtgcaccgccgctttacaggcttcgacgccgcttcgttctaccatcgacaccaccacgctggcacccagttgatcggcgcgagatttaatcgccgcgacaatttgcgacggcgcgtgcagggccagactggaggtggcaacgccaatcagcaacgactgtttgcccgccagttgttgtgccacgcggttgggaatgtaattcagctccgccatcgccgcttccactttttcccgcgttttcgcagaaacgtggctggcctggttcaccacgcgggaaacggtctgataagagacaccggcatactctgcgacatcgtataacgttactggtttcacattcaccaccctgaattgactctcttccgggcgctatcatgccataccgcgaaaggttttgcgccattcgatggtgtcggatcgagatcgatctcgatcccgcgaaattaatacgactcactataggggaattgtgagcggataacaattcctactagagattaaagaggagaaattaattatgaaatatctgctgccgaccgctgctgctggtctgctgctcctcgctgcccagccggcgatggcccgtaaaggcgaagagctgttcactggtgtcgtccctattctggtggaactggatggtgatgtcaacg…
encoding: naseq.html

https://synbiohub.org/public/SEGA/SEGA090_seq/1
type: Sequence
persistent identity: SEGA090 seq
display id: SEGA090_seq
version: 1
owned by: carbay
owned by: morno
elements: gtgcatggtctgcaggcgctgccgagccgtattgagcagatgctgtctcaggacaaacgcattgaagcgctggcagaagatttctctgacaaacatcacgcgctgttcctgggccgtggcgatcagtacccaatcgcgctggaaggcgcattgaagttgaaagagatctcttacattcacgctgaagcctacgctgctggcgaactgaaacacggtccgctggcgctaattgatgccgatatgccggttattgttgttgcaccgaacaacgaattgctggaaaaactgaaatccaacattgaagaagttcgcgcgcgtggcggtcagttgtatgtcttcgccgatcaggatgcgggttttgtaagtagcgataacatgcacatcatcgagatgccgcatgtggaagaggtgattgcaccgatcttctacaccgttccgctgcagctgctggcttaccatgtcgcgctgatcaaaggcaccgacgttgaccagccgcgtaacctggcaaaatcggttacggttgagtaataaatggatctcggtaccaaattccagaaaagagacgctttcgagcgtcttttttcgttttggtccaccgtattcatcagcactgtcctgctccttgtgatggtttacaaacgtaaaaagtctctttaatacctgtttttgcttcatattgttcagcgacagcttgctgtacggcaggcaccagctcttccgggatcagcgcgacgatacagccgccaaatccgccgccggtcatgcgtacgccacctttgtcgccaatcacagctttgacgatttctaccagagtgtcaatttgcggcacggtgatttcgaaatcatcgcgcatagaggcatgagactccgccatcaactcgcccatacgtttcaggtcgccttgctccagcgcgctggcagcttcaacggtgcgggcgttttcagtcagtatatgacgcacgcgttttgccacgatcgggtccagttcatgcgcaacagcgttgaactcttcaatggtgacatcacgcagggctggctgctggaagaaacgcgcaccggtttcgcactgttcacgacgggtgttgtattcgctgccaaccagggtacgtttgaagttactgttgatgatgacgacagccacacctttgggcatggaaactgctttggtccccagtgagcggcaatcgatcagcaaggcatgatctttcttgccgagcgcggaaattagctgatccatgatcccgcagttacagcctacaaactggttttctgcttcctgaccgttaagcgcgatttgtgcgccgtccagcggcagatgataaagctgctgcaatacggttccgaccgcgacttccagtgaagcggaagaacttaacccggcaccctgcggcacattgccgctgatcaccatgtccacgccgccgaagctgttgttacgcagttgcagatgtttcaccacgccacgaacgtagttagcccattgatagttttcatgtgcgacaatgggcgcatcgagggaaaactcgtcgagctgattttcataatcggctgccatcacgcgaactttacggtcatcgcgtggtgcacaactgatcacggtttgataatcaatcgcgcagggcagaacgaaaccgtcgttgtagtcggtgtgttcaccaatcaaattcacgcggccaggcgcctgaatggtgtgagtggcagggtagccaaatgcgttggcaaacagagattgtgttttttctttcagactcatgattgcctcctgggttagttcctcaccttgtcgtattatactatgccgatatactatgccgatgattaattgtcaacctttaatgaattataaaacgaaaggctcagtcgaaagactgggcctttcgttttatgcgttgcgcttaatctttctgcgaattgagatgacgccactggctgggcgtcatcccggtttcccgggtaaacaccaccgaaaaatagttactatcttcaaagccacattcggt…
encoding: naseq.html

https://synbiohub.org/public/SEGA/SEGA011_seq/1
type: Sequence
persistent identity: SEGA011 seq
display id: SEGA011_seq
version: 1
owned by: carbay
owned by: morno
elements: gtgcatggtctgcaggcgctgccgagccgtattgagcagatgctgtctcaggacaaacgcattgaagcgctggcagaagatttctctgacaaacatcacgcgctgttcctgggccgtggcgatcagtacccaatcgcgctggaaggcgcattgaagttgaaagagatctcttacattcacgctgaagcctacgctgctggcgaactgaaacacggtccgctggcgctaattgatgccgatatgccggttattgttgttgcaccgaacaacgaattgctggaaaaactgaaatccaacattgaagaagttcgcgcgcgtggcggtcagttgtatgtcttcgccgatcaggatgcgggttttgtaagtagcgataacatgcacatcatcgagatgccgcatgtggaagaggtgattgcaccgatcttctacaccgttccgctgcagctgctggcttaccatgtcgcgctgatcaaaggcaccgacgttgaccagccgcgtaacctggcaaaatcggttacggttgagtaataaatggatctcggtaccaaattccagaaaagagacgctttcgagcgtcttttttcgttttggtccgcgttgcgcttgacggctagctcagtcctaggtacagtgctagctactagagattaaagaggagaaacgggggatggagaacctgtacttccagagcaaaggagaagaacttttcactggagttgtgccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgctacatacggaaaactcacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactctgacctatggcgttcaatgcttttcccgttatccggatcacatgaaacggcatgactttttcaagagtgccatgcccgaaggttatgtacaggaacgcactatatctttcaaagatgacgggaactacaagacgcgtgctgaagtcaagtttgaaggtgatacccttgttaatcgtatcgagttaaagggtattgattttaaagaagatggaaacattctcggacacaaactagagtacaactataactcacacaatgtatacatcacggcagacaaacaaaagaatggaatcaaagctaacttcaaaattcgccacaacattgaagatggttccgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtcgacacaatctgccctttcgaaagatcccaacgaaaagcgtgaccacatggtccttcttgagtttgtaactgctgctgggattacacatggcatggatgagctctacaaatgaccaattattgaaggccgctaacgcggcctttttttgtttctggtctcccaaaaatttatttgcttattaatcatccggctcgtataatgtgtggagggcccaagttcacttaaaaaggagaatagttatgaaatcgaacaatgcgctcatcgtcatcctcggcaccgtcaccctggatgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagcatcgccagtcactatggcgtgctgctagcgctatatgcgttgatgcaatttctatgcgcacccgttctcggagcactgtccgaccgctttggccgccgcccagtcctgctcgcttcgctacttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggattctctacgccggacgcatcgtggccggcatcaccggcgccacaggtgcggttgctggcgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggcgccatc…
encoding: naseq.html

https://synbiohub.org/public/SEGA/SEGA026_seq/1
type: Sequence
persistent identity: SEGA026 seq
display id: SEGA026_seq
version: 1
owned by: carbay
owned by: morno
elements: gtgcatggtctgcaggcgctgccgagccgtattgagcagatgctgtctcaggacaaacgcattgaagcgctggcagaagatttctctgacaaacatcacgcgctgttcctgggccgtggcgatcagtacccaatcgcgctggaaggcgcattgaagttgaaagagatctcttacattcacgctgaagcctacgctgctggcgaactgaaacacggtccgctggcgctaattgatgccgatatgccggttattgttgttgcaccgaacaacgaattgctggaaaaactgaaatccaacattgaagaagttcgcgcgcgtggcggtcagttgtatgtcttcgccgatcaggatgcgggttttgtaagtagcgataacatgcacatcatcgagatgccgcatgtggaagaggtgattgcaccgatcttctacaccgttccgctgcagctgctggcttaccatgtcgcgctgatcaaaggcaccgacgttgaccagccgcgtaacctggcaaaatcggttacggttgagtaataaatggatctcggtaccaaattccagaaaagagacgctttcgagcgtcttttttcgttttggtccgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgccagggtggtttttcttttcaccagtgagacgggcaacagctgattgcccttcaccgcctggccctgagagagttgcagcaagcggtccacgctggtttgccccagcaggcgaaaatcctgtttgatggtggttaacggcgggatataacatgagctgtcttcggtatcgtcgtatcccactaccgagatgtccgcaccaacgcgcagcccggactcggtaatggcgcgcattgcgcccagcgccatctgatcgttggcaaccagcatcgcagtgggaacgatgccctcattcagcatttgcatggtttgttgaaaaccggacatggcactccagtcgccttcccgttccgctatcggctgaatttgattgcgagtgagatatttatgccagccagccagacgcagacgcgccgagacagaacttaatgggcccgctaacagcgcgatttgctggtgacccaatgcgaccagatgctccacgcccagtcgcgtaccgtcttcatgggagaaaataatactgttgatgggtgtctggtcagagacatcaagaaataacgccggaacattagtgcaggcagcttccacagcaatggcatcctggtcatccagcggatagttaatgatcagcccactgacgcgttgcgcgagaagattgtgcaccgccgctttacaggcttcgacgccgcttcgttctaccatcgacaccaccacgctggcacccagttgatcggcgcgagatttaatcgccgcgacaatttgcgacggcgcgtgcagggccagactggaggtggcaacgccaatcagcaacgactgtttgcccgccagttgttgtgccacgcggttgggaatgtaattcagctccgccatcgccgcttccactttttcccgcgttttcgcagaaacgtggctggcctggttcaccacgcgggaaacggtctgataagagacaccggcatactctgcgacatcgtataacgttactggtttcacattcaccaccctgaattgactctcttccgggcgctatcatgccataccgcgaaaggttttgcgccattcgatggtgtcggatcgagatcgatctcgatcccgcgaaatttgacaattaatcatccggctcgtataatggaattgtgagcggataacaattcctactagagattaaagaggagaaacgggggatggagaacctgtacttccagagcaaaggagaagaacttttcactggagttgtgccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggt…
encoding: naseq.html

https://synbiohub.org/public/SEGA/SEGA099_seq/1
type: Sequence
persistent identity: SEGA099 seq
display id: SEGA099_seq
version: 1
owned by: carbay
owned by: morno
elements: gtgcatggtctgcaggcgctgccgagccgtattgagcagatgctgtctcaggacaaacgcattgaagcgctggcagaagatttctctgacaaacatcacgcgctgttcctgggccgtggcgatcagtacccaatcgcgctggaaggcgcattgaagttgaaagagatctcttacattcacgctgaagcctacgctgctggcgaactgaaacacggtccgctggcgctaattgatgccgatatgccggttattgttgttgcaccgaacaacgaattgctggaaaaactgaaatccaacattgaagaagttcgcgcgcgtggcggtcagttgtatgtcttcgccgatcaggatgcgggttttgtaagtagcgataacatgcacatcatcgagatgccgcatgtggaagaggtgattgcaccgatcttctacaccgttccgctgcagctgctggcttaccatgtcgcgctgatcaaaggcaccgacgttgaccagccgcgtaacctggcaaaatcggttacggttgagtaataaatggatctcggtaccaaattccagaaaagagacgctttcgagcgtcttttttcgttttggtccgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgccagggtggtttttcttttcaccagtgagacgggcaacagctgattgcccttcaccgcctggccctgagagagttgcagcaagcggtccacgctggtttgccccagcaggcgaaaatcctgtttgatggtggttaacggcgggatataacatgagctgtcttcggtatcgtcgtatcccactaccgagatgtccgcaccaacgcgcagcccggactcggtaatggcgcgcattgcgcccagcgccatctgatcgttggcaaccagcatcgcagtgggaacgatgccctcattcagcatttgcatggtttgttgaaaaccggacatggcactccagtcgccttcccgttccgctatcggctgaatttgattgcgagtgagatatttatgccagccagccagacgcagacgcgccgagacagaacttaatgggcccgctaacagcgcgatttgctggtgacccaatgcgaccagatgctccacgcccagtcgcgtaccgtcttcatgggagaaaataatactgttgatgggtgtctggtcagagacatcaagaaataacgccggaacattagtgcaggcagcttccacagcaatggcatcctggtcatccagcggatagttaatgatcagcccactgacgcgttgcgcgagaagattgtgcaccgccgctttacaggcttcgacgccgcttcgttctaccatcgacaccaccacgctggcacccagttgatcggcgcgagatttaatcgccgcgacaatttgcgacggcgcgtgcagggccagactggaggtggcaacgccaatcagcaacgactgtttgcccgccagttgttgtgccacgcggttgggaatgtaattcagctccgccatcgccgcttccactttttcccgcgttttcgcagaaacgtggctggcctggttcaccacgcgggaaacggtctgataagagacaccggcatactctgcgacatcgtataacgttactggtttcacattcaccaccctgaattgactctcttccgggcgctatcatgccataccgcgaaaggttttgcgccattcgatggtgtcggatcgagatcgatctcgatcccgcgaaatttgacaattaatcatccggctcgtataatggaattgtgagcggataacaattcctactagagattaaagaggagaaaggggggatgaaggctattttcgtactgaaacatcttaatcatgctaaggaggttttctaatggtactgccgggcctcttgcgggatatcgtccattccgacagcatcgccagtcactatggcgtgctgc…
encoding: naseq.html

https://synbiohub.org/public/SEGA/SEGA004_seq/1
type: Sequence
persistent identity: SEGA004 seq
display id: SEGA004_seq
version: 1
owned by: carbay
owned by: morno
elements: gtgcatggtctgcaggcgctgccgagccgtattgagcagatgctgtctcaggacaaacgcattgaagcgctggcagaagatttctctgacaaacatcacgcgctgttcctgggccgtggcgatcagtacccaatcgcgctggaaggcgcattgaagttgaaagagatctcttacattcacgctgaagcctacgctgctggcgaactgaaacacggtccgctggcgctaattgatgccgatatgccggttattgttgttgcaccgaacaacgaattgctggaaaaactgaaatccaacattgaagaagttcgcgcgcgtggcggtcagttgtatgtcttcgccgatcaggatgcgggttttgtaagtagcgataacatgcacatcatcgagatgccgcatgtggaagaggtgattgcaccgatcttctacaccgttccgctgcagctgctggcttaccatgtcgcgctgatcaaaggcaccgacgttgaccagccgcgtaacctggcaaaatcggttacggttgagtaataaatggatctcggtaccaaattccagaaaagagacgctttcgagcgtcttttttcgttttggtccgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgccagggtggtttttcttttcaccagtgagacgggcaacagctgattgcccttcaccgcctggccctgagagagttgcagcaagcggtccacgctggtttgccccagcaggcgaaaatcctgtttgatggtggttaacggcgggatataacatgagctgtcttcggtatcgtcgtatcccactaccgagatgtccgcaccaacgcgcagcccggactcggtaatggcgcgcattgcgcccagcgccatctgatcgttggcaaccagcatcgcagtgggaacgatgccctcattcagcatttgcatggtttgttgaaaaccggacatggcactccagtcgccttcccgttccgctatcggctgaatttgattgcgagtgagatatttatgccagccagccagacgcagacgcgccgagacagaacttaatgggcccgctaacagcgcgatttgctggtgacccaatgcgaccagatgctccacgcccagtcgcgtaccgtcttcatgggagaaaataatactgttgatgggtgtctggtcagagacatcaagaaataacgccggaacattagtgcaggcagcttccacagcaatggcatcctggtcatccagcggatagttaatgatcagcccactgacgcgttgcgcgagaagattgtgcaccgccgctttacaggcttcgacgccgcttcgttctaccatcgacaccaccacgctggcacccagttgatcggcgcgagatttaatcgccgcgacaatttgcgacggcgcgtgcagggccagactggaggtggcaacgccaatcagcaacgactgtttgcccgccagttgttgtgccacgcggttgggaatgtaattcagctccgccatcgccgcttccactttttcccgcgttttcgcagaaacgtggctggcctggttcaccacgcgggaaacggtctgataagagacaccggcatactctgcgacatcgtataacgttactggtttcacattcaccaccctgaattgactctcttccgggcgctatcatgccataccgcgaaaggttttgcgccattcgatggtgtcggatcgagatcgatctcgatcccgcgaaattaatacgactcactataggggaattgtgagcggataacaattcctactagagattaaagaggagaaagtactgccgggcctcttgcgggatatcgtccattccgacagcatcgccagtcactatggcgtgctgctagcgctatatgcgttgatgcaatttctatgcgcacccgttctcggagcactgtccgaccgctttggccgcc…
encoding: naseq.html

https://synbiohub.org/public/SEGA/SEGA040_seq/1
type: Sequence
persistent identity: SEGA040 seq
display id: SEGA040_seq
version: 1
owned by: carbay
owned by: morno
elements: gtgcatggtctgcaggcgctgccgagccgtattgagcagatgctgtctcaggacaaacgcattgaagcgctggcagaagatttctctgacaaacatcacgcgctgttcctgggccgtggcgatcagtacccaatcgcgctggaaggcgcattgaagttgaaagagatctcttacattcacgctgaagcctacgctgctggcgaactgaaacacggtccgctggcgctaattgatgccgatatgccggttattgttgttgcaccgaacaacgaattgctggaaaaactgaaatccaacattgaagaagttcgcgcgcgtggcggtcagttgtatgtcttcgccgatcaggatgcgggttttgtaagtagcgataacatgcacatcatcgagatgccgcatgtggaagaggtgattgcaccgatcttctacaccgttccgctgcagctgctggcttaccatgtcgcgctgatcaaaggcaccgacgttgaccagccgcgtaacctggcaaaatcggttacggttgagtaataaatggatctcggtaccaaattccagaaaagagacgctttcgagcgtcttttttcgttttggtccgcgttgcgcttaatctttctgcgaattgagatgacgccactggctgggcgtcatcccggtttcccgggtaaacaccaccgaaaaatagttactatcttcaaagccacattcggtcgaaatatcactgattaacaggcggctatgctggagaagatattgcgcatgacacactctgacctgtcgcagatattgattgatggtcattccagtctgctggcgaaattgctgacgcaaaacgcgctcactgcacgatgcctcatcacaaaatttatccagcgcaaagggacttttcaggctagccgccagccgggtaatcagcttatccagcaacgtttcgctggatgttggcggcaacgaatcactggtgtaacgatggcgattcagcaacatcaccaactgcccgaacagcaactcagccatttcgttagcaaacggcacatgctgactactttcatgctcaagctgaccaataacctgccgcgcctgcgccatccccatgctacctaagcgccagtgtggttgccctgcgctggcgttaaatcccggaatcgccccctgccagtcaagattcagcttcagacgctccgggcaataaataatattctgcaaaaccagatcgttaacggaagcgtaggagtgtttatcatcagcatgaatgtaaaagagatcgccacgggtaatgcgataagggcgatcgttgagtacatgcaggccattaccgcgccagacaatcaccagctcacaaaaatcatgtgtatgttcagcaaagacatcttgcggataacggtcagccacagcgactgcctgctggtcgctggcaaaaaaatcatctttgagaagttttaactgatgcgccaccgtggctacctcggccagagaacgaagttgattattcgcaatatggcgtacaaatacgttgagaagattcgcgttattgcagaaagccatcccgtccctggcgaatatcacgcggtgaccagttaaactctcggcgaaaaagcgtcgaaaagtggttactgtcgctgaatccacagcgataggcgatgtcagtaacgctggcctcgctgtggcgtagcagatgtcgggctttcatcagtcgcaggcggttcaggtatcgctgaggcgtcagtcccgtttgctgcttaagctgccgatgtagcgtacgcagtgaaagagaaaattgatccgccacggcatcccaattcacctcatcggcaaaatggtcctccagccaggccagaagcaagttgagacgtgatgcgctgttttccaggttctcctgcaaactgcttttacgcagcaagagcagtaattgcataaacaagatctcgcgactggcggtcgagggtaaatcattttccccttcctgctgttccatctgtgcaaccagctgtcgcacctgctgc…
encoding: naseq.html

https://synbiohub.org/public/SEGA/SEGA001_seq/1
type: Sequence
persistent identity: SEGA001 seq
display id: SEGA001_seq
version: 1
owned by: carbay
owned by: morno
elements: gtgcatggtctgcaggcgctgccgagccgtattgagcagatgctgtctcaggacaaacgcattgaagcgctggcagaagatttctctgacaaacatcacgcgctgttcctgggccgtggcgatcagtacccaatcgcgctggaaggcgcattgaagttgaaagagatctcttacattcacgctgaagcctacgctgctggcgaactgaaacacggtccgctggcgctaattgatgccgatatgccggttattgttgttgcaccgaacaacgaattgctggaaaaactgaaatccaacattgaagaagttcgcgcgcgtggcggtcagttgtatgtcttcgccgatcaggatgcgggttttgtaagtagcgataacatgcacatcatcgagatgccgcatgtggaagaggtgattgcaccgatcttctacaccgttccgctgcagctgctggcttaccatgtcgcgctgatcaaaggcaccgacgttgaccagccgcgtaacctggcaaaatcggttacggttgagtaataaatggatctcggtaccaaattccagaaaagagacgctttcgagcgtcttttttcgttttggtccgcgttgcgcttgacggctagctcagtcctaggtacagtgctagctactagagattaaagaggagaaagtactgccgggcctcttgcgggatatcgtccattccgacagcatcgccagtcactatggcgtgctgctagcgctatatgcgttgatgcaatttctatgcgcacccgttctcggagcactgtccgaccgctttggccgccgcccagtcctgctcgcttcgctacttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggattctctacgccggacgcatcgtggccggcatcaccggcgccacaggtgcggttgctggcgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggcgccatctccctgcacgcaccattccttgcggcggcggtgctcaacggcctcaacctactactgggctgcttcctaatgcaggagtcgcataagggagagcgccgtccgatgcccttgagagccttcaacccagtcagctccttccggtgggcgcggggcatgaccattgtggccgcacttatgactgtcttctttatcatgcaactcgtaggacaggtgccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgagcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcactggtcccgccaccaaacgtttcggcgagaagcaggccattatcgccggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgaggctggatggccttccccattatgattcttctcgcttccggcggcatcgggatgcccgcgttgcaggccatgctgtccaggcaggtagatgacgaccatcagggacagcttcaaggatcgctcgcggctcttaccagcctaacttcgatcactggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggcgccgccctataccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctgagacaattgtccagataaaaaaaatccttagctttcgctaaggatgatttctaaaatttttctgtcatctcttcgttattaatgtttgtaattgactgaatatcaacgcttatttaaatcagactgaagactttatctctctgtcataaaactgtcatattccttacatataactgtcacctgtttgtcctattttgcttctcgtagccaacaaacaatgctttatg…
encoding: naseq.html

https://synbiohub.org/public/SEGA/SEGA066_seq/1
type: Sequence
persistent identity: SEGA066 seq
display id: SEGA066_seq
version: 1
owned by: carbay
owned by: morno
elements: gtgcatggtctgcaggcgctgccgagccgtattgagcagatgctgtctcaggacaaacgcattgaagcgctggcagaagatttctctgacaaacatcacgcgctgttcctgggccgtggcgatcagtacccaatcgcgctggaaggcgcattgaagttgaaagagatctcttacattcacgctgaagcctacgctgctggcgaactgaaacacggtccgctggcgctaattgatgccgatatgccggttattgttgttgcaccgaacaacgaattgctggaaaaactgaaatccaacattgaagaagttcgcgcgcgtggcggtcagttgtatgtcttcgccgatcaggatgcgggttttgtaagtagcgataacatgcacatcatcgagatgccgcatgtggaagaggtgattgcaccgatcttctacaccgttccgctgcagctgctggcttaccatgtcgcgctgatcaaaggcaccgacgttgaccagccgcgtaacctggcaaaatcggttacggttgagtaataaatggatctcggtaccaaattccagaaaagagacgctttcgagcgtcttttttcgttttggtccgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgccagggtggtttttcttttcaccagtgagacgggcaacagctgattgcccttcaccgcctggccctgagagagttgcagcaagcggtccacgctggtttgccccagcaggcgaaaatcctgtttgatggtggttaacggcgggatataacatgagctgtcttcggtatcgtcgtatcccactaccgagatgtccgcaccaacgcgcagcccggactcggtaatggcgcgcattgcgcccagcgccatctgatcgttggcaaccagcatcgcagtgggaacgatgccctcattcagcatttgcatggtttgttgaaaaccggacatggcactccagtcgccttcccgttccgctatcggctgaatttgattgcgagtgagatatttatgccagccagccagacgcagacgcgccgagacagaacttaatgggcccgctaacagcgcgatttgctggtgacccaatgcgaccagatgctccacgcccagtcgcgtaccgtcttcatgggagaaaataatactgttgatgggtgtctggtcagagacatcaagaaataacgccggaacattagtgcaggcagcttccacagcaatggcatcctggtcatccagcggatagttaatgatcagcccactgacgcgttgcgcgagaagattgtgcaccgccgctttacaggcttcgacgccgcttcgttctaccatcgacaccaccacgctggcacccagttgatcggcgcgagatttaatcgccgcgacaatttgcgacggcgcgtgcagggccagactggaggtggcaacgccaatcagcaacgactgtttgcccgccagttgttgtgccacgcggttgggaatgtaattcagctccgccatcgccgcttccactttttcccgcgttttcgcagaaacgtggctggcctggttcaccacgcgggaaacggtctgataagagacaccggcatactctgcgacatcgtataacgttactggtttcacattcaccaccctgaattgactctcttccgggcgctatcatgccataccgcgaaaggttttgcgccattcgatggtgtcggatcgagatcgatctcgatcccgcgaaattaatacgactcactataggggaattgtgagcggataacaattcctactagagattaaagaggagaaaaactacatggaaaacctgtacttccagtttcgtagcaaaggagaagaacttgtcactggagttagcaaaggagaagaacttttcactggagttgtgccaattcttgttgaattagatggtgatgttaatgggcacaaat…
encoding: naseq.html

https://synbiohub.org/public/SEGA/SEGA025_seq/1
type: Sequence
persistent identity: SEGA025 seq
display id: SEGA025_seq
version: 1
owned by: carbay
owned by: morno
elements: gtgcatggtctgcaggcgctgccgagccgtattgagcagatgctgtctcaggacaaacgcattgaagcgctggcagaagatttctctgacaaacatcacgcgctgttcctgggccgtggcgatcagtacccaatcgcgctggaaggcgcattgaagttgaaagagatctcttacattcacgctgaagcctacgctgctggcgaactgaaacacggtccgctggcgctaattgatgccgatatgccggttattgttgttgcaccgaacaacgaattgctggaaaaactgaaatccaacattgaagaagttcgcgcgcgtggcggtcagttgtatgtcttcgccgatcaggatgcgggttttgtaagtagcgataacatgcacatcatcgagatgccgcatgtggaagaggtgattgcaccgatcttctacaccgttccgctgcagctgctggcttaccatgtcgcgctgatcaaaggcaccgacgttgaccagccgcgtaacctggcaaaatcggttacggttgagtaataaatggatctcggtaccaaattccagaaaagagacgctttcgagcgtcttttttcgttttggtccgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgccagggtggtttttcttttcaccagtgagacgggcaacagctgattgcccttcaccgcctggccctgagagagttgcagcaagcggtccacgctggtttgccccagcaggcgaaaatcctgtttgatggtggttaacggcgggatataacatgagctgtcttcggtatcgtcgtatcccactaccgagatgtccgcaccaacgcgcagcccggactcggtaatggcgcgcattgcgcccagcgccatctgatcgttggcaaccagcatcgcagtgggaacgatgccctcattcagcatttgcatggtttgttgaaaaccggacatggcactccagtcgccttcccgttccgctatcggctgaatttgattgcgagtgagatatttatgccagccagccagacgcagacgcgccgagacagaacttaatgggcccgctaacagcgcgatttgctggtgacccaatgcgaccagatgctccacgcccagtcgcgtaccgtcttcatgggagaaaataatactgttgatgggtgtctggtcagagacatcaagaaataacgccggaacattagtgcaggcagcttccacagcaatggcatcctggtcatccagcggatagttaatgatcagcccactgacgcgttgcgcgagaagattgtgcaccgccgctttacaggcttcgacgccgcttcgttctaccatcgacaccaccacgctggcacccagttgatcggcgcgagatttaatcgccgcgacaatttgcgacggcgcgtgcagggccagactggaggtggcaacgccaatcagcaacgactgtttgcccgccagttgttgtgccacgcggttgggaatgtaattcagctccgccatcgccgcttccactttttcccgcgttttcgcagaaacgtggctggcctggttcaccacgcgggaaacggtctgataagagacaccggcatactctgcgacatcgtataacgttactggtttcacattcaccaccctgaattgactctcttccgggcgctatcatgccataccgcgaaaggttttgcgccattcgatggtgtcggatcgagatcgatctcgatcccgcgaaatttgacaattaatcatccggctcgtataatggaattgtgagcggataacaattcctactagagattaaagaggagaaacattgtatgcagatattcgtgaaaacactgaccggcaaaaccattaccctggaggtggagcctggcagcgcccatcatcatcaccatcaccatcaccatcatgctggtagcagcgatacgatcgaaaac…
encoding: naseq.html

https://synbiohub.org/public/SEGA/SEGA006_seq/1
type: Sequence
persistent identity: SEGA006 seq
display id: SEGA006_seq
version: 1
owned by: carbay
owned by: morno
elements: gtgcatggtctgcaggcgctgccgagccgtattgagcagatgctgtctcaggacaaacgcattgaagcgctggcagaagatttctctgacaaacatcacgcgctgttcctgggccgtggcgatcagtacccaatcgcgctggaaggcgcattgaagttgaaagagatctcttacattcacgctgaagcctacgctgctggcgaactgaaacacggtccgctggcgctaattgatgccgatatgccggttattgttgttgcaccgaacaacgaattgctggaaaaactgaaatccaacattgaagaagttcgcgcgcgtggcggtcagttgtatgtcttcgccgatcaggatgcgggttttgtaagtagcgataacatgcacatcatcgagatgccgcatgtggaagaggtgattgcaccgatcttctacaccgttccgctgcagctgctggcttaccatgtcgcgctgatcaaaggcaccgacgttgaccagccgcgtaacctggcaaaatcggttacggttgagtaataaatggatctcggtaccaaattccagaaaagagacgctttcgagcgtcttttttcgttttggtccgcgttgcgcttgacggctagctcagtcctaggtacagtgctagctactagagattaaagaggagaaagagctaatgaaggctattttcgtactgaaacatcttaatcatgctaaggaggttttctaatgagcaaaggagaagaacttttcactggagttgtgccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgctacatacggaaaactcacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactctgacctatggcgttcaatgcttttcccgttatccggatcacatgaaacggcatgactttttcaagagtgccatgcccgaaggttatgtacaggaacgcactatatctttcaaagatgacgggaactacaagacgcgtgctgaagtcaagtttgaaggtgatacccttgttaatcgtatcgagttaaagggtattgattttaaagaagatggaaacattctcggacacaaactagagtacaactataactcacacaatgtatacatcacggcagacaaacaaaagaatggaatcaaagctaacttcaaaattcgccacaacattgaagatggttccgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtcgacacaatctgccctttcgaaagatcccaacgaaaagcgtgaccacatggtccttcttgagtttgtaactgctgctgggattacacatggcatggatgagctctacaaatgaccaattattgaaggccgctaacgcggcctttttttgtttctggtctcccaaaaatttatttgcttattaatcatccggctcgtataatgtgtggagggcccaagttcacttaaaaaggagaatagttatgaaatcgaacaatgcgctcatcgtcatcctcggcaccgtcaccctggatgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagcatcgccagtcactatggcgtgctgctagcgctatatgcgttgatgcaatttctatgcgcacccgttctcggagcactgtccgaccgctttggccgccgcccagtcctgctcgcttcgctacttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggattctctacgccggacgcatcgtggccggcatcaccggcgccacaggtgcggttgctggcgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtgg…
encoding: naseq.html

https://synbiohub.org/public/SEGA/SEGA036_seq/1
type: Sequence
persistent identity: SEGA036 seq
display id: SEGA036_seq
version: 1
owned by: carbay
owned by: morno
elements: gtgcatggtctgcaggcgctgccgagccgtattgagcagatgctgtctcaggacaaacgcattgaagcgctggcagaagatttctctgacaaacatcacgcgctgttcctgggccgtggcgatcagtacccaatcgcgctggaaggcgcattgaagttgaaagagatctcttacattcacgctgaagcctacgctgctggcgaactgaaacacggtccgctggcgctaattgatgccgatatgccggttattgttgttgcaccgaacaacgaattgctggaaaaactgaaatccaacattgaagaagttcgcgcgcgtggcggtcagttgtatgtcttcgccgatcaggatgcgggttttgtaagtagcgataacatgcacatcatcgagatgccgcatgtggaagaggtgattgcaccgatcttctacaccgttccgctgcagctgctggcttaccatgtcgcgctgatcaaaggcaccgacgttgaccagccgcgtaacctggcaaaatcggttacggttgagtaataaatggatctcggtaccaaattccagaaaagagacgctttcgagcgtcttttttcgttttggtccgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgccagggtggtttttcttttcaccagtgagacgggcaacagctgattgcccttcaccgcctggccctgagagagttgcagcaagcggtccacgctggtttgccccagcaggcgaaaatcctgtttgatggtggttaacggcgggatataacatgagctgtcttcggtatcgtcgtatcccactaccgagatgtccgcaccaacgcgcagcccggactcggtaatggcgcgcattgcgcccagcgccatctgatcgttggcaaccagcatcgcagtgggaacgatgccctcattcagcatttgcatggtttgttgaaaaccggacatggcactccagtcgccttcccgttccgctatcggctgaatttgattgcgagtgagatatttatgccagccagccagacgcagacgcgccgagacagaacttaatgggcccgctaacagcgcgatttgctggtgacccaatgcgaccagatgctccacgcccagtcgcgtaccgtcttcatgggagaaaataatactgttgatgggtgtctggtcagagacatcaagaaataacgccggaacattagtgcaggcagcttccacagcaatggcatcctggtcatccagcggatagttaatgatcagcccactgacgcgttgcgcgagaagattgtgcaccgccgctttacaggcttcgacgccgcttcgttctaccatcgacaccaccacgctggcacccagttgatcggcgcgagatttaatcgccgcgacaatttgcgacggcgcgtgcagggccagactggaggtggcaacgccaatcagcaacgactgtttgcccgccagttgttgtgccacgcggttgggaatgtaattcagctccgccatcgccgcttccactttttcccgcgttttcgcagaaacgtggctggcctggttcaccacgcgggaaacggtctgataagagacaccggcatactctgcgacatcgtataacgttactggtttcacattcaccaccctgaattgactctcttccgggcgctatcatgccataccgcgaaaggttttgcgccattcgatggtgtcggatcgagatcgatctcgatcccgcgaaatttgacaattaatcatccggctcgtataatggaattgtgagcggataacaattcctactagagattaaagaggagaaagactggatgaaaaagagaggggcgtttttagggctgttgttggtttctgcctgcgcatcagttttcgctgcgaataacgaaacgagcaagagcgtcaccttcccgaagtgcgaggatctggacgcagcg…
encoding: naseq.html

https://synbiohub.org/public/SEGA/SEGA074_seq/1
type: Sequence
persistent identity: SEGA074 seq
display id: SEGA074_seq
version: 1
owned by: carbay
owned by: morno
elements: gtgcatggtctgcaggcgctgccgagccgtattgagcagatgctgtctcaggacaaacgcattgaagcgctggcagaagatttctctgacaaacatcacgcgctgttcctgggccgtggcgatcagtacccaatcgcgctggaaggcgcattgaagttgaaagagatctcttacattcacgctgaagcctacgctgctggcgaactgaaacacggtccgctggcgctaattgatgccgatatgccggttattgttgttgcaccgaacaacgaattgctggaaaaactgaaatccaacattgaagaagttcgcgcgcgtggcggtcagttgtatgtcttcgccgatcaggatgcgggttttgtaagtagcgataacatgcacatcatcgagatgccgcatgtggaagaggtgattgcaccgatcttctacaccgttccgctgcagctgctggcttaccatgtcgcgctgatcaaaggcaccgacgttgaccagccgcgtaacctggcaaaatcggttacggttgagtaataaatggatctcggtaccaaattccagaaaagagacgctttcgagcgtcttttttcgttttggtccccaattaggatccttatcaatccgtaaacaggtcaaacatcagttgccgcaaccaaatattggctaggtccttgtggtacttcgcatgccagaacatgttgatggctatttcaggcaagacgactgggtgcggcaaggcgcttaggccgaagggctctacgcagcagtcggctaaacatatcggcacagtggcgagcagatcggtgcgctggaggatgtggccaacggcggcgaagtgcggcacttccagacggatgtcgcgccggatgccgacccgtgtcatgtacgtgtccacctcgccgtggccggtgccagcggcgatgacacgcacgtggccgtaggaacagaagcgctccagagtcaggggttcgcgggtgactggatggtccttgcgacataggcacacgtagtgattctggagcagccggcgctgaaagaagccagtttgcagattgggaagcaggcccacggccaagtccacggttccgttctgcaaggcctgcatcaggctcatcgaactgtcgcgcaccgtactgatcacgcaattgggggcctggtgagccagcgcatccatcagccgcggcatgaagtagatctcgccaatgtcggtcatggccagggtgaaggtacgctcgctggtcagcggatcgaagctttcatggtgctgtagggcgttgcgcagtgcgtgcatggccgaagtgacgtgctcggccagatgcgcggcatagggtgtgggttccattccctgatgtgtgcgcacgaagagtgggtcctgtagcgaggtgcgcaggcgtttcagcgcattgctcacggcaggctgggtcaggcccaggttctccgcagtgacagagacgcgtctgtcgaccagcaactggttgaacaccaccagcaggtttaaatccaggtcacgcagttccatattcaacccccatgaattgactctcttccgggcgctatcatgccataccgcgaaaggttttgcaccattcgatggcgcgccgccattggagcttaacgatcgttggctgggggcctcgcttgggttattgctggtgcccggccgggcgcaatattcatgttgatgatttattatatatcgagtggtgtatttatttatattgtttgctccgttaccgttattaacagctgtcaccggatgtgctttccggtctgatgagtccgtgaggacgaaacagcctctacaaataattttgtttaatactagagattaaagaggagaaaggggggatgaaggctattttcgtactgaaacatcttaatcatgctaaggaggttttctaatgagcaaaggagaagaacttttcactggagttgtgccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggt…
encoding: naseq.html

https://synbiohub.org/public/SEGA/SEGA042_seq/1
type: Sequence
persistent identity: SEGA042 seq
display id: SEGA042_seq
version: 1
owned by: carbay
owned by: morno
elements: gtgcatggtctgcaggcgctgccgagccgtattgagcagatgctgtctcaggacaaacgcattgaagcgctggcagaagatttctctgacaaacatcacgcgctgttcctgggccgtggcgatcagtacccaatcgcgctggaaggcgcattgaagttgaaagagatctcttacattcacgctgaagcctacgctgctggcgaactgaaacacggtccgctggcgctaattgatgccgatatgccggttattgttgttgcaccgaacaacgaattgctggaaaaactgaaatccaacattgaagaagttcgcgcgcgtggcggtcagttgtatgtcttcgccgatcaggatgcgggttttgtaagtagcgataacatgcacatcatcgagatgccgcatgtggaagaggtgattgcaccgatcttctacaccgttccgctgcagctgctggcttaccatgtcgcgctgatcaaaggcaccgacgttgaccagccgcgtaacctggcaaaatcggttacggttgagtaataaatggatctcggtaccaaattccagaaaagagacgctttcgagcgtcttttttcgttttggtccgcgttgcgcttaatctttctgcgaattgagatgacgccactggctgggcgtcatcccggtttcccgggtaaacaccaccgaaaaatagttactatcttcaaagccacattcggtcgaaatatcactgattaacaggcggctatgctggagaagatattgcgcatgacacactctgacctgtcgcagatattgattgatggtcattccagtctgctggcgaaattgctgacgcaaaacgcgctcactgcacgatgcctcatcacaaaatttatccagcgcaaagggacttttcaggctagccgccagccgggtaatcagcttatccagcaacgtttcgctggatgttggcggcaacgaatcactggtgtaacgatggcgattcagcaacatcaccaactgcccgaacagcaactcagccatttcgttagcaaacggcacatgctgactactttcatgctcaagctgaccaataacctgccgcgcctgcgccatccccatgctacctaagcgccagtgtggttgccctgcgctggcgttaaatcccggaatcgccccctgccagtcaagattcagcttcagacgctccgggcaataaataatattctgcaaaaccagatcgttaacggaagcgtaggagtgtttatcatcagcatgaatgtaaaagagatcgccacgggtaatgcgataagggcgatcgttgagtacatgcaggccattaccgcgccagacaatcaccagctcacaaaaatcatgtgtatgttcagcaaagacatcttgcggataacggtcagccacagcgactgcctgctggtcgctggcaaaaaaatcatctttgagaagttttaactgatgcgccaccgtggctacctcggccagagaacgaagttgattattcgcaatatggcgtacaaatacgttgagaagattcgcgttattgcagaaagccatcccgtccctggcgaatatcacgcggtgaccagttaaactctcggcgaaaaagcgtcgaaaagtggttactgtcgctgaatccacagcgataggcgatgtcagtaacgctggcctcgctgtggcgtagcagatgtcgggctttcatcagtcgcaggcggttcaggtatcgctgaggcgtcagtcccgtttgctgcttaagctgccgatgtagcgtacgcagtgaaagagaaaattgatccgccacggcatcccaattcacctcatcggcaaaatggtcctccagccaggccagaagcaagttgagacgtgatgcgctgttttccaggttctcctgcaaactgcttttacgcagcaagagcagtaattgcataaacaagatctcgcgactggcggtcgagggtaaatcattttccccttcctgctgttccatctgtgcaaccagctgtcgcacctgctgc…
encoding: naseq.html

https://synbiohub.org/public/SEGA/SEGA046_seq/1
type: Sequence
persistent identity: SEGA046 seq
display id: SEGA046_seq
version: 1
owned by: carbay
owned by: morno
elements: gtgcatggtctgcaggcgctgccgagccgtattgagcagatgctgtctcaggacaaacgcattgaagcgctggcagaagatttctctgacaaacatcacgcgctgttcctgggccgtggcgatcagtacccaatcgcgctggaaggcgcattgaagttgaaagagatctcttacattcacgctgaagcctacgctgctggcgaactgaaacacggtccgctggcgctaattgatgccgatatgccggttattgttgttgcaccgaacaacgaattgctggaaaaactgaaatccaacattgaagaagttcgcgcgcgtggcggtcagttgtatgtcttcgccgatcaggatgcgggttttgtaagtagcgataacatgcacatcatcgagatgccgcatgtggaagaggtgattgcaccgatcttctacaccgttccgctgcagctgctggcttaccatgtcgcgctgatcaaaggcaccgacgttgaccagccgcgtaacctggcaaaatcggttacggttgagtaataaatggatctcggtaccaaattccagaaaagagacgctttcgagcgtcttttttcgttttggtccgcgttgcgcttaatctttctgcgaattgagatgacgccactggctgggcgtcatcccggtttcccgggtaaacaccaccgaaaaatagttactatcttcaaagccacattcggtcgaaatatcactgattaacaggcggctatgctggagaagatattgcgcatgacacactctgacctgtcgcagatattgattgatggtcattccagtctgctggcgaaattgctgacgcaaaacgcgctcactgcacgatgcctcatcacaaaatttatccagcgcaaagggacttttcaggctagccgccagccgggtaatcagcttatccagcaacgtttcgctggatgttggcggcaacgaatcactggtgtaacgatggcgattcagcaacatcaccaactgcccgaacagcaactcagccatttcgttagcaaacggcacatgctgactactttcatgctcaagctgaccaataacctgccgcgcctgcgccatccccatgctacctaagcgccagtgtggttgccctgcgctggcgttaaatcccggaatcgccccctgccagtcaagattcagcttcagacgctccgggcaataaataatattctgcaaaaccagatcgttaacggaagcgtaggagtgtttatcatcagcatgaatgtaaaagagatcgccacgggtaatgcgataagggcgatcgttgagtacatgcaggccattaccgcgccagacaatcaccagctcacaaaaatcatgtgtatgttcagcaaagacatcttgcggataacggtcagccacagcgactgcctgctggtcgctggcaaaaaaatcatctttgagaagttttaactgatgcgccaccgtggctacctcggccagagaacgaagttgattattcgcaatatggcgtacaaatacgttgagaagattcgcgttattgcagaaagccatcccgtccctggcgaatatcacgcggtgaccagttaaactctcggcgaaaaagcgtcgaaaagtggttactgtcgctgaatccacagcgataggcgatgtcagtaacgctggcctcgctgtggcgtagcagatgtcgggctttcatcagtcgcaggcggttcaggtatcgctgaggcgtcagtcccgtttgctgcttaagctgccgatgtagcgtacgcagtgaaagagaaaattgatccgccacggcatcccaattcacctcatcggcaaaatggtcctccagccaggccagaagcaagttgagacgtgatgcgctgttttccaggttctcctgcaaactgcttttacgcagcaagagcagtaattgcataaacaagatctcgcgactggcggtcgagggtaaatcattttccccttcctgctgttccatctgtgcaaccagctgtcgcacctgctgc…
encoding: naseq.html

https://synbiohub.org/public/SEGA/SEGA003_seq/1
type: Sequence
persistent identity: SEGA003 seq
display id: SEGA003_seq
version: 1
owned by: carbay
owned by: morno
elements: gtgcatggtctgcaggcgctgccgagccgtattgagcagatgctgtctcaggacaaacgcattgaagcgctggcagaagatttctctgacaaacatcacgcgctgttcctgggccgtggcgatcagtacccaatcgcgctggaaggcgcattgaagttgaaagagatctcttacattcacgctgaagcctacgctgctggcgaactgaaacacggtccgctggcgctaattgatgccgatatgccggttattgttgttgcaccgaacaacgaattgctggaaaaactgaaatccaacattgaagaagttcgcgcgcgtggcggtcagttgtatgtcttcgccgatcaggatgcgggttttgtaagtagcgataacatgcacatcatcgagatgccgcatgtggaagaggtgattgcaccgatcttctacaccgttccgctgcagctgctggcttaccatgtcgcgctgatcaaaggcaccgacgttgaccagccgcgtaacctggcaaaatcggttacggttgagtaataaatggatctcggtaccaaattccagaaaagagacgctttcgagcgtcttttttcgttttggtccgcgttgcgcttaatctttctgcgaattgagatgacgccactggctgggcgtcatcccggtttcccgggtaaacaccaccgaaaaatagttactatcttcaaagccacattcggtcgaaatatcactgattaacaggcggctatgctggagaagatattgcgcatgacacactctgacctgtcgcagatattgattgatggtcattccagtctgctggcgaaattgctgacgcaaaacgcgctcactgcacgatgcctcatcacaaaatttatccagcgcaaagggacttttcaggctagccgccagccgggtaatcagcttatccagcaacgtttcgctggatgttggcggcaacgaatcactggtgtaacgatggcgattcagcaacatcaccaactgcccgaacagcaactcagccatttcgttagcaaacggcacatgctgactactttcatgctcaagctgaccaataacctgccgcgcctgcgccatccccatgctacctaagcgccagtgtggttgccctgcgctggcgttaaatcccggaatcgccccctgccagtcaagattcagcttcagacgctccgggcaataaataatattctgcaaaaccagatcgttaacggaagcgtaggagtgtttatcatcagcatgaatgtaaaagagatcgccacgggtaatgcgataagggcgatcgttgagtacatgcaggccattaccgcgccagacaatcaccagctcacaaaaatcatgtgtatgttcagcaaagacatcttgcggataacggtcagccacagcgactgcctgctggtcgctggcaaaaaaatcatctttgagaagttttaactgatgcgccaccgtggctacctcggccagagaacgaagttgattattcgcaatatggcgtacaaatacgttgagaagattcgcgttattgcagaaagccatcccgtccctggcgaatatcacgcggtgaccagttaaactctcggcgaaaaagcgtcgaaaagtggttactgtcgctgaatccacagcgataggcgatgtcagtaacgctggcctcgctgtggcgtagcagatgtcgggctttcatcagtcgcaggcggttcaggtatcgctgaggcgtcagtcccgtttgctgcttaagctgccgatgtagcgtacgcagtgaaagagaaaattgatccgccacggcatcccaattcacctcatcggcaaaatggtcctccagccaggccagaagcaagttgagacgtgatgcgctgttttccaggttctcctgcaaactgcttttacgcagcaagagcagtaattgcataaacaagatctcgcgactggcggtcgagggtaaatcattttccccttcctgctgttccatctgtgcaaccagctgtcgcacctgctgc…
encoding: naseq.html

https://synbiohub.org/public/SEGA/SEGA032_seq/1
type: Sequence
persistent identity: SEGA032 seq
display id: SEGA032_seq
version: 1
owned by: carbay
owned by: morno
elements: gtgcatggtctgcaggcgctgccgagccgtattgagcagatgctgtctcaggacaaacgcattgaagcgctggcagaagatttctctgacaaacatcacgcgctgttcctgggccgtggcgatcagtacccaatcgcgctggaaggcgcattgaagttgaaagagatctcttacattcacgctgaagcctacgctgctggcgaactgaaacacggtccgctggcgctaattgatgccgatatgccggttattgttgttgcaccgaacaacgaattgctggaaaaactgaaatccaacattgaagaagttcgcgcgcgtggcggtcagttgtatgtcttcgccgatcaggatgcgggttttgtaagtagcgataacatgcacatcatcgagatgccgcatgtggaagaggtgattgcaccgatcttctacaccgttccgctgcagctgctggcttaccatgtcgcgctgatcaaaggcaccgacgttgaccagccgcgtaacctggcaaaatcggttacggttgagtaataaatggatctcggtaccaaattccagaaaagagacgctttcgagcgtcttttttcgttttggtccgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgccagggtggtttttcttttcaccagtgagacgggcaacagctgattgcccttcaccgcctggccctgagagagttgcagcaagcggtccacgctggtttgccccagcaggcgaaaatcctgtttgatggtggttaacggcgggatataacatgagctgtcttcggtatcgtcgtatcccactaccgagatgtccgcaccaacgcgcagcccggactcggtaatggcgcgcattgcgcccagcgccatctgatcgttggcaaccagcatcgcagtgggaacgatgccctcattcagcatttgcatggtttgttgaaaaccggacatggcactccagtcgccttcccgttccgctatcggctgaatttgattgcgagtgagatatttatgccagccagccagacgcagacgcgccgagacagaacttaatgggcccgctaacagcgcgatttgctggtgacccaatgcgaccagatgctccacgcccagtcgcgtaccgtcttcatgggagaaaataatactgttgatgggtgtctggtcagagacatcaagaaataacgccggaacattagtgcaggcagcttccacagcaatggcatcctggtcatccagcggatagttaatgatcagcccactgacgcgttgcgcgagaagattgtgcaccgccgctttacaggcttcgacgccgcttcgttctaccatcgacaccaccacgctggcacccagttgatcggcgcgagatttaatcgccgcgacaatttgcgacggcgcgtgcagggccagactggaggtggcaacgccaatcagcaacgactgtttgcccgccagttgttgtgccacgcggttgggaatgtaattcagctccgccatcgccgcttccactttttcccgcgttttcgcagaaacgtggctggcctggttcaccacgcgggaaacggtctgataagagacaccggcatactctgcgacatcgtataacgttactggtttcacattcaccaccctgaattgactctcttccgggcgctatcatgccataccgcgaaaggttttgcgccattcgatggtgtcggatcgagatcgatctcgatcccgcgaaatttgacaattaatcatccggctcgtataatggaattgtgagcggataacaattcctactagagattaaagaggagaaacggacgatgaagtacctgctgccgaccgctgctgctggtctgctgctcctcgctgcccagccggcgatggcccgtaaaggcgaagagctgttcactggtgtcgtccctattctggtggaactggatggt…
encoding: naseq.html

https://synbiohub.org/public/SEGA/SEGA096_seq/1
type: Sequence
persistent identity: SEGA096 seq
display id: SEGA096_seq
version: 1
owned by: carbay
owned by: morno
elements: gtgcatggtctgcaggcgctgccgagccgtattgagcagatgctgtctcaggacaaacgcattgaagcgctggcagaagatttctctgacaaacatcacgcgctgttcctgggccgtggcgatcagtacccaatcgcgctggaaggcgcattgaagttgaaagagatctcttacattcacgctgaagcctacgctgctggcgaactgaaacacggtccgctggcgctaattgatgccgatatgccggttattgttgttgcaccgaacaacgaattgctggaaaaactgaaatccaacattgaagaagttcgcgcgcgtggcggtcagttgtatgtcttcgccgatcaggatgcgggttttgtaagtagcgataacatgcacatcatcgagatgccgcatgtggaagaggtgattgcaccgatcttctacaccgttccgctgcagctgctggcttaccatgtcgcgctgatcaaaggcaccgacgttgaccagccgcgtaacctggcaaaatcggttacggttgagtaataaatggatctcggtaccaaattccagaaaagagacgctttcgagcgtcttttttcgttttggtccgcgttgcgcttgacggctagctcagtcctaggtacagtgctagctactagagattaaagaggagaaaggggggatgaaggctattttcgtactgaaacatcttaatcatgctaaggaggttttctaatggtactgccgggcctcttgcgggatatcgtccattccgacagcatcgccagtcactatggcgtgctgctagcgctatatgcgttgatgcaatttctatgcgcacccgttctcggagcactgtccgaccgctttggccgccgcccagtcctgctcgcttcgctacttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggattctctacgccggacgcatcgtggccggcatcaccggcgccacaggtgcggttgctggcgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggcgccatctccctgcacgcaccattccttgcggcggcggtgctcaacggcctcaacctactactgggctgcttcctaatgcaggagtcgcataagggagagcgccgtccgatgcccttgagagccttcaacccagtcagctccttccggtgggcgcggggcatgaccattgtggccgcacttatgactgtcttctttatcatgcaactcgtaggacaggtgccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgagcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcactggtcccgccaccaaacgtttcggcgagaagcaggccattatcgccggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgaggctggatggccttccccattatgattcttctcgcttccggcggcatcgggatgcccgcgttgcaggccatgctgtccaggcaggtagatgacgaccatcagggacagcttcaaggatcgctcgcggctcttaccagcctaacttcgatcactggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggcgccgccctataccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctgagacaattgtccagataaaaaaaatccttagctttcgctaaggatgatttctaaaatttttctgtcatctcttcgttattaatgtttgtaattgactgaatatcaacgcttatttaaatcagactgaagactttatctctctgtcataaaactgtcatattcctt…
encoding: naseq.html

https://synbiohub.org/public/SEGA/SEGA067_seq/1
type: Sequence
persistent identity: SEGA067 seq
display id: SEGA067_seq
version: 1
owned by: carbay
owned by: morno
elements: gtgcatggtctgcaggcgctgccgagccgtattgagcagatgctgtctcaggacaaacgcattgaagcgctggcagaagatttctctgacaaacatcacgcgctgttcctgggccgtggcgatcagtacccaatcgcgctggaaggcgcattgaagttgaaagagatctcttacattcacgctgaagcctacgctgctggcgaactgaaacacggtccgctggcgctaattgatgccgatatgccggttattgttgttgcaccgaacaacgaattgctggaaaaactgaaatccaacattgaagaagttcgcgcgcgtggcggtcagttgtatgtcttcgccgatcaggatgcgggttttgtaagtagcgataacatgcacatcatcgagatgccgcatgtggaagaggtgattgcaccgatcttctacaccgttccgctgcagctgctggcttaccatgtcgcgctgatcaaaggcaccgacgttgaccagccgcgtaacctggcaaaatcggttacggttgagtaataaatggatctcggtaccaaattccagaaaagagacgctttcgagcgtcttttttcgttttggtccgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgccagggtggtttttcttttcaccagtgagacgggcaacagctgattgcccttcaccgcctggccctgagagagttgcagcaagcggtccacgctggtttgccccagcaggcgaaaatcctgtttgatggtggttaacggcgggatataacatgagctgtcttcggtatcgtcgtatcccactaccgagatgtccgcaccaacgcgcagcccggactcggtaatggcgcgcattgcgcccagcgccatctgatcgttggcaaccagcatcgcagtgggaacgatgccctcattcagcatttgcatggtttgttgaaaaccggacatggcactccagtcgccttcccgttccgctatcggctgaatttgattgcgagtgagatatttatgccagccagccagacgcagacgcgccgagacagaacttaatgggcccgctaacagcgcgatttgctggtgacccaatgcgaccagatgctccacgcccagtcgcgtaccgtcttcatgggagaaaataatactgttgatgggtgtctggtcagagacatcaagaaataacgccggaacattagtgcaggcagcttccacagcaatggcatcctggtcatccagcggatagttaatgatcagcccactgacgcgttgcgcgagaagattgtgcaccgccgctttacaggcttcgacgccgcttcgttctaccatcgacaccaccacgctggcacccagttgatcggcgcgagatttaatcgccgcgacaatttgcgacggcgcgtgcagggccagactggaggtggcaacgccaatcagcaacgactgtttgcccgccagttgttgtgccacgcggttgggaatgtaattcagctccgccatcgccgcttccactttttcccgcgttttcgcagaaacgtggctggcctggttcaccacgcgggaaacggtctgataagagacaccggcatactctgcgacatcgtataacgttactggtttcacattcaccaccctgaattgactctcttccgggcgctatcatgccataccgcgaaaggttttgcgccattcgatggtgtcggatcgagatcgatctcgatcccgcgaaattaatacgactcactataggggaattgtgagcggataacaattcctactagagattaaagaggagaaaatatcgatggagaacctgtacttccagtttcgtagcaaaggagaagaacttgtcactggagttagcaaaggagaagaacttttcactggagttgtgccaattcttgttgaattagatggtgatgttaatgggcacaaat…
encoding: naseq.html

https://synbiohub.org/public/SEGA/SEGA092_seq/1
type: Sequence
persistent identity: SEGA092 seq
display id: SEGA092_seq
version: 1
owned by: carbay
owned by: morno
elements: gtgcatggtctgcaggcgctgccgagccgtattgagcagatgctgtctcaggacaaacgcattgaagcgctggcagaagatttctctgacaaacatcacgcgctgttcctgggccgtggcgatcagtacccaatcgcgctggaaggcgcattgaagttgaaagagatctcttacattcacgctgaagcctacgctgctggcgaactgaaacacggtccgctggcgctaattgatgccgatatgccggttattgttgttgcaccgaacaacgaattgctggaaaaactgaaatccaacattgaagaagttcgcgcgcgtggcggtcagttgtatgtcttcgccgatcaggatgcgggttttgtaagtagcgataacatgcacatcatcgagatgccgcatgtggaagaggtgattgcaccgatcttctacaccgttccgctgcagctgctggcttaccatgtcgcgctgatcaaaggcaccgacgttgaccagccgcgtaacctggcaaaatcggttacggttgagtaataaatggatctcggtaccaaattccagaaaagagacgctttcgagcgtcttttttcgttttggtccaccgtattcatcagcactgtcctgctccttgtgatggtttacaaacgtaaaaagtctctttaatacctgtttttgcttcatattgttcagcgacagcttgctgtacggcaggcaccagctcttccgggatcagcgcgacgatacagccgccaaatccgccgccggtcatgcgtacgccacctttgtcgccaatcacagctttgacgatttctaccagagtgtcaatttgcggcacggtgatttcgaaatcatcgcgcatagaggcatgagactccgccatcaactcgcccatacgtttcaggtcgccttgctccagcgcgctggcagcttcaacggtgcgggcgttttcagtcagtatatgacgcacgcgttttgccacgatcgggtccagttcatgcgcaacagcgttgaactcttcaatggtgacatcacgcagggctggctgctggaagaaacgcgcaccggtttcgcactgttcacgacgggtgttgtattcgctgccaaccagggtacgtttgaagttactgttgatgatgacgacagccacacctttgggcatggaaactgctttggtccccagtgagcggcaatcgatcagcaaggcatgatctttcttgccgagcgcggaaattagctgatccatgatcccgcagttacagcctacaaactggttttctgcttcctgaccgttaagcgcgatttgtgcgccgtccagcggcagatgataaagctgctgcaatacggttccgaccgcgacttccagtgaagcggaagaacttaacccggcaccctgcggcacattgccgctgatcaccatgtccacgccgccgaagctgttgttacgcagttgcagatgtttcaccacgccacgaacgtagttagcccattgatagttttcatgtgcgacaatgggcgcatcgagggaaaactcgtcgagctgattttcataatcggctgccatcacgcgaactttacggtcatcgcgtggtgcacaactgatcacggtttgataatcaatcgcgcagggcagaacgaaaccgtcgttgtagtcggtgtgttcaccaatcaaattcacgcggccaggcgcctgaatggtgtgagtggcagggtagccaaatgcgttggcaaacagagattgtgttttttctttcagactcatgattgcctcctgggttagttcctcaccttgtcgtattatactatgccgatatactatgccgatgattaattgtcaacctttaatgaattataaaacgaaaggctcagtcgaaagactgggcctttcgttttatgcgttgcgcttaatctttctgcgaattgagatgacgccactggctgggcgtcatcccggtttcccgggtaaacaccaccgaaaaatagttactatcttcaaagccacattcggt…
encoding: naseq.html

https://synbiohub.org/public/SEGA/SEGA078_seq/1
type: Sequence
persistent identity: SEGA078 seq
display id: SEGA078_seq
version: 1
owned by: carbay
owned by: morno
elements: gtgcatggtctgcaggcgctgccgagccgtattgagcagatgctgtctcaggacaaacgcattgaagcgctggcagaagatttctctgacaaacatcacgcgctgttcctgggccgtggcgatcagtacccaatcgcgctggaaggcgcattgaagttgaaagagatctcttacattcacgctgaagcctacgctgctggcgaactgaaacacggtccgctggcgctaattgatgccgatatgccggttattgttgttgcaccgaacaacgaattgctggaaaaactgaaatccaacattgaagaagttcgcgcgcgtggcggtcagttgtatgtcttcgccgatcaggatgcgggttttgtaagtagcgataacatgcacatcatcgagatgccgcatgtggaagaggtgattgcaccgatcttctacaccgttccgctgcagctgctggcttaccatgtcgcgctgatcaaaggcaccgacgttgaccagccgcgtaacctggcaaaatcggttacggttgagtaataaatggatctcggtaccaaattccagaaaagagacgctttcgagcgtcttttttcgttttggtccaccgtattcatcagcactgtcctgctccttgtgatggtttacaaacgtaaagagtctctttaatacctgtttttgcttcatattgttcagcgacagcttgctgtacggcaggcaccagctcttccgggatcagcgcgacgatacagccgccaaatccgccgccggtcatgcgtacgccacctttgtcgccaatcacagctttgacgatttctaccagagtgtcaatttgcggcacggtgatttcgaaatcatcgcgcatagaggcatgagactccgccatcaactcgcccatacgtttcaggtcgccttgctccagcgcgctggcagcttcaacggtgcgggcgttttcagtcagtatatgacgcacgcgttttgccacgatcgggtccagttcatgcgcaacagcgttgaactcttcaatggtgacatcacgcagggctggctgctggaagaaacgcgcaccggtttcgcactgttcacgacgggtgttgtattcgctgccaaccagggtacgtttgaagttactgttgatgatgacgacagccacacctttgggcatggaaactgctttggtccccagtgagcggcaatcgatcagcaaggcatgatctttcttgccgagcgcggaaattagctgatccatgatcccgcagttacagcctacaaactggttttctgcttcctgaccgttaagcgcgatttgtgcgccgtccagcggcagatgataaagctgctgcaatacggttccgaccgcgacttccagtgaagcggaagaacttaacccggcaccctgcggcacattgccgctgatcaccatgtccacgccgccgaagctgttgttacgcagttgcagatgtttcaccacgccacgaacgtagttagcccattgatagttttcatgtgcgacaatgggcgcatcgagggaaaactcgtcgagctgattttcataatcggctgccatcacgcgaactttacggtcatcgcgtggtgcacaactgatcacggtttgataatcaatcgcgcagggcagaacgaaaccgtcgttgtagtcggtgtgttcaccaatcaaattcacgcggccaggcgcctgaatggtgtgagtggcagggtagccaaatgcgttggcaaacagagattgtgttttttctttcagactcatgattgcctcctgggttagttcctcaccttgtcgtattatactatgccgatatactatgccgatgattaattgtcaacctttaatgaattataaaacgaaaggctcagtcgaaagactgggcctttcgttttatgcgttgcgcttaatctttctgcgaattgagatgacgccactggctgggcgtcatcccggtttcccgggtaaacaccaccgaaaaatagttactatcttcaaagccacattcggt…
encoding: naseq.html

https://synbiohub.org/public/SEGA/SEGA068_seq/1
type: Sequence
persistent identity: SEGA068 seq
display id: SEGA068_seq
version: 1
owned by: carbay
owned by: morno
elements: gtgcatggtctgcaggcgctgccgagccgtattgagcagatgctgtctcaggacaaacgcattgaagcgctggcagaagatttctctgacaaacatcacgcgctgttcctgggccgtggcgatcagtacccaatcgcgctggaaggcgcattgaagttgaaagagatctcttacattcacgctgaagcctacgctgctggcgaactgaaacacggtccgctggcgctaattgatgccgatatgccggttattgttgttgcaccgaacaacgaattgctggaaaaactgaaatccaacattgaagaagttcgcgcgcgtggcggtcagttgtatgtcttcgccgatcaggatgcgggttttgtaagtagcgataacatgcacatcatcgagatgccgcatgtggaagaggtgattgcaccgatcttctacaccgttccgctgcagctgctggcttaccatgtcgcgctgatcaaaggcaccgacgttgaccagccgcgtaacctggcaaaatcggttacggttgagtaataaatggatctcggtaccaaattccagaaaagagacgctttcgagcgtcttttttcgttttggtccgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgccagggtggtttttcttttcaccagtgagacgggcaacagctgattgcccttcaccgcctggccctgagagagttgcagcaagcggtccacgctggtttgccccagcaggcgaaaatcctgtttgatggtggttaacggcgggatataacatgagctgtcttcggtatcgtcgtatcccactaccgagatgtccgcaccaacgcgcagcccggactcggtaatggcgcgcattgcgcccagcgccatctgatcgttggcaaccagcatcgcagtgggaacgatgccctcattcagcatttgcatggtttgttgaaaaccggacatggcactccagtcgccttcccgttccgctatcggctgaatttgattgcgagtgagatatttatgccagccagccagacgcagacgcgccgagacagaacttaatgggcccgctaacagcgcgatttgctggtgacccaatgcgaccagatgctccacgcccagtcgcgtaccgtcttcatgggagaaaataatactgttgatgggtgtctggtcagagacatcaagaaataacgccggaacattagtgcaggcagcttccacagcaatggcatcctggtcatccagcggatagttaatgatcagcccactgacgcgttgcgcgagaagattgtgcaccgccgctttacaggcttcgacgccgcttcgttctaccatcgacaccaccacgctggcacccagttgatcggcgcgagatttaatcgccgcgacaatttgcgacggcgcgtgcagggccagactggaggtggcaacgccaatcagcaacgactgtttgcccgccagttgttgtgccacgcggttgggaatgtaattcagctccgccatcgccgcttccactttttcccgcgttttcgcagaaacgtggctggcctggttcaccacgcgggaaacggtctgataagagacaccggcatactctgcgacatcgtataacgttactggtttcacattcaccaccctgaattgactctcttccgggcgctatcatgccataccgcgaaaggttttgcgccattcgatggtgtcggatcgagatcgatctcgatcccgcgaaattaatacgactcactataggggaattgtgagcggataacaattcctactagagattaaagaggagaaacggacgatgaagtacctgctgccgaccgctgctgctggtctgctgctcctcgctgcccagccggcgatggcccgtaaaggcgaagagctgttcactggtgtcgtccctattctggtggaactggatggtgatgtcaacg…
encoding: naseq.html

https://synbiohub.org/public/SEGA/SEGA009_seq/1
type: Sequence
persistent identity: SEGA009 seq
display id: SEGA009_seq
version: 1
owned by: carbay
owned by: morno
elements: gtgcatggtctgcaggcgctgccgagccgtattgagcagatgctgtctcaggacaaacgcattgaagcgctggcagaagatttctctgacaaacatcacgcgctgttcctgggccgtggcgatcagtacccaatcgcgctggaaggcgcattgaagttgaaagagatctcttacattcacgctgaagcctacgctgctggcgaactgaaacacggtccgctggcgctaattgatgccgatatgccggttattgttgttgcaccgaacaacgaattgctggaaaaactgaaatccaacattgaagaagttcgcgcgcgtggcggtcagttgtatgtcttcgccgatcaggatgcgggttttgtaagtagcgataacatgcacatcatcgagatgccgcatgtggaagaggtgattgcaccgatcttctacaccgttccgctgcagctgctggcttaccatgtcgcgctgatcaaaggcaccgacgttgaccagccgcgtaacctggcaaaatcggttacggttgagtaataaatggatctcggtaccaaattccagaaaagagacgctttcgagcgtcttttttcgttttggtccgcgttgcgcttgacggctagctcagtcctaggtacagtgctagctactagagattaaagaggagaaaggctggatgcaaatattcgtgaaaacactgaccggcaaaaccattaccctggaggtggagcctggcagcgcccatcatcatcaccatcaccatcaccatcatgctggtagcagcgatacgatcgaaaacgtcaaagccaaaattcaggacaaagagggcattccgcctgatcaacaacgtctgatcttcgccggaaaacagctggaggatggccgtacactgagtgactataacattcaagagaaatcgacactggaactggttctggagctgcagaatgaaacccagtcagcaacttctgacgcttctggcggtagcaaaggagaagaacttttcactggagttgtgccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgctacatacggaaaactcacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactctgacctatggcgttcaatgcttttcccgttatccggatcacatgaaacggcatgactttttcaagagtgccatgcccgaaggttatgtacaggaacgcactatatctttcaaagatgacgggaactacaagacgcgtgctgaagtcaagtttgaaggtgatacccttgttaatcgtatcgagttaaagggtattgattttaaagaagatggaaacattctcggacacaaactagagtacaactataactcacacaatgtatacatcacggcagacaaacaaaagaatggaatcaaagctaacttcaaaattcgccacaacattgaagatggttccgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtcgacacaatctgccctttcgaaagatcccaacgaaaagcgtgaccacatggtccttcttgagtttgtaactgctgctgggattacacatggcatggatgagctctacaaatgaccaattattgaaggccgctaacgcggcctttttttgtttctggtctcccaaaaatttatttgcttattaatcatccggctcgtataatgtgtggagggcccaagttcacttaaaaaggagaatagttatgaaatcgaacaatgcgctcatcgtcatcctcggcaccgtcaccctggatgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagcatcgccagtcactatggcgtgctgctagcgctatatgcgttgatgcaatttctatgcgcaccc…
encoding: naseq.html

https://synbiohub.org/public/SEGA/SEGA088_seq/1
type: Sequence
persistent identity: SEGA088 seq
display id: SEGA088_seq
version: 1
owned by: carbay
owned by: morno
elements: gtgcatggtctgcaggcgctgccgagccgtattgagcagatgctgtctcaggacaaacgcattgaagcgctggcagaagatttctctgacaaacatcacgcgctgttcctgggccgtggcgatcagtacccaatcgcgctggaaggcgcattgaagttgaaagagatctcttacattcacgctgaagcctacgctgctggcgaactgaaacacggtccgctggcgctaattgatgccgatatgccggttattgttgttgcaccgaacaacgaattgctggaaaaactgaaatccaacattgaagaagttcgcgcgcgtggcggtcagttgtatgtcttcgccgatcaggatgcgggttttgtaagtagcgataacatgcacatcatcgagatgccgcatgtggaagaggtgattgcaccgatcttctacaccgttccgctgcagctgctggcttaccatgtcgcgctgatcaaaggcaccgacgttgaccagccgcgtaacctggcaaaatcggttacggttgagtaataaatggatctcggtaccaaattccagaaaagagacgctttcgagcgtcttttttcgttttggtccaccgtattcatcagcactgtcctgctccttgtgatggtttacaaacgtaaaaagtctctttaatacctgtttttgcttcatattgttcagcgacagcttgctgtacggcaggcaccagctcttccgggatcagcgcgacgatacagccgccaaatccgccgccggtcatgcgtacgccacctttgtcgccaatcacagctttgacgatttctaccagagtgtcaatttgcggcacggtgatttcgaaatcatcgcgcatagaggcatgagactccgccatcaactcgcccatacgtttcaggtcgccttgctccagcgcgctggcagcttcaacggtgcgggcgttttcagtcagtatatgacgcacgcgttttgccacgatcgggtccagttcatgcgcaacagcgttgaactcttcaatggtgacatcacgcagggctggctgctggaagaaacgcgcaccggtttcgcactgttcacgacgggtgttgtattcgctgccaaccagggtacgtttgaagttactgttgatgatgacgacagccacacctttgggcatggaaactgctttggtccccagtgagcggcaatcgatcagcaaggcatgatctttcttgccgagcgcggaaattagctgatccatgatcccgcagttacagcctacaaactggttttctgcttcctgaccgttaagcgcgatttgtgcgccgtccagcggcagatgataaagctgctgcaatacggttccgaccgcgacttccagtgaagcggaagaacttaacccggcaccctgcggcacattgccgctgatcaccatgtccacgccgccgaagctgttgttacgcagttgcagatgtttcaccacgccacgaacgtagttagcccattgatagttttcatgtgcgacaatgggcgcatcgagggaaaactcgtcgagctgattttcataatcggctgccatcacgcgaactttacggtcatcgcgtggtgcacaactgatcacggtttgataatcaatcgcgcagggcagaacgaaaccgtcgttgtagtcggtgtgttcaccaatcaaattcacgcggccaggcgcctgaatggtgtgagtggcagggtagccaaatgcgttggcaaacagagattgtgttttttctttcagactcatgattgcctcctgggttagttcctcaccttgtcgtattatactatgccgatatactatgccgatgattaattgtcaacctttaatgaattataaaacgaaaggctcagtcgaaagactgggcctttcgttttatgcgttgcgcttaatctttctgcgaattgagatgacgccactggctgggcgtcatcccggtttcccgggtaaacaccaccgaaaaatagttactatcttcaaagccacattcggt…
encoding: naseq.html

https://synbiohub.org/public/SEGA/SEGA024_seq/1
type: Sequence
persistent identity: SEGA024 seq
display id: SEGA024_seq
version: 1
owned by: carbay
owned by: morno
elements: gtgcatggtctgcaggcgctgccgagccgtattgagcagatgctgtctcaggacaaacgcattgaagcgctggcagaagatttctctgacaaacatcacgcgctgttcctgggccgtggcgatcagtacccaatcgcgctggaaggcgcattgaagttgaaagagatctcttacattcacgctgaagcctacgctgctggcgaactgaaacacggtccgctggcgctaattgatgccgatatgccggttattgttgttgcaccgaacaacgaattgctggaaaaactgaaatccaacattgaagaagttcgcgcgcgtggcggtcagttgtatgtcttcgccgatcaggatgcgggttttgtaagtagcgataacatgcacatcatcgagatgccgcatgtggaagaggtgattgcaccgatcttctacaccgttccgctgcagctgctggcttaccatgtcgcgctgatcaaaggcaccgacgttgaccagccgcgtaacctggcaaaatcggttacggttgagtaataaatggatctcggtaccaaattccagaaaagagacgctttcgagcgtcttttttcgttttggtccgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgccagggtggtttttcttttcaccagtgagacgggcaacagctgattgcccttcaccgcctggccctgagagagttgcagcaagcggtccacgctggtttgccccagcaggcgaaaatcctgtttgatggtggttaacggcgggatataacatgagctgtcttcggtatcgtcgtatcccactaccgagatgtccgcaccaacgcgcagcccggactcggtaatggcgcgcattgcgcccagcgccatctgatcgttggcaaccagcatcgcagtgggaacgatgccctcattcagcatttgcatggtttgttgaaaaccggacatggcactccagtcgccttcccgttccgctatcggctgaatttgattgcgagtgagatatttatgccagccagccagacgcagacgcgccgagacagaacttaatgggcccgctaacagcgcgatttgctggtgacccaatgcgaccagatgctccacgcccagtcgcgtaccgtcttcatgggagaaaataatactgttgatgggtgtctggtcagagacatcaagaaataacgccggaacattagtgcaggcagcttccacagcaatggcatcctggtcatccagcggatagttaatgatcagcccactgacgcgttgcgcgagaagattgtgcaccgccgctttacaggcttcgacgccgcttcgttctaccatcgacaccaccacgctggcacccagttgatcggcgcgagatttaatcgccgcgacaatttgcgacggcgcgtgcagggccagactggaggtggcaacgccaatcagcaacgactgtttgcccgccagttgttgtgccacgcggttgggaatgtaattcagctccgccatcgccgcttccactttttcccgcgttttcgcagaaacgtggctggcctggttcaccacgcgggaaacggtctgataagagacaccggcatactctgcgacatcgtataacgttactggtttcacattcaccaccctgaattgactctcttccgggcgctatcatgccataccgcgaaaggttttgcgccattcgatggtgtcggatcgagatcgatctcgatcccgcgaaatttgacaattaatcatccggctcgtataatggaattgtgagcggataacaattcctactagagattaaagaggagaaaggctggatgcaaatattcgtgaaaacactgaccggcaaaaccattaccctggaggtggagcctggcagcgcccatcatcatcaccatcaccatcaccatcatgctggtagcagcgatacgatcgaaaac…
encoding: naseq.html

https://synbiohub.org/public/SEGA/SEGA093_seq/1
type: Sequence
persistent identity: SEGA093 seq
display id: SEGA093_seq
version: 1
owned by: carbay
owned by: morno
elements: gtgcatggtctgcaggcgctgccgagccgtattgagcagatgctgtctcaggacaaacgcattgaagcgctggcagaagatttctctgacaaacatcacgcgctgttcctgggccgtggcgatcagtacccaatcgcgctggaaggcgcattgaagttgaaagagatctcttacattcacgctgaagcctacgctgctggcgaactgaaacacggtccgctggcgctaattgatgccgatatgccggttattgttgttgcaccgaacaacgaattgctggaaaaactgaaatccaacattgaagaagttcgcgcgcgtggcggtcagttgtatgtcttcgccgatcaggatgcgggttttgtaagtagcgataacatgcacatcatcgagatgccgcatgtggaagaggtgattgcaccgatcttctacaccgttccgctgcagctgctggcttaccatgtcgcgctgatcaaaggcaccgacgttgaccagccgcgtaacctggcaaaatcggttacggttgagtaataaatggatctcggtaccaaattccagaaaagagacgctttcgagcgtcttttttcgttttggtccaccgtattcatcagcactgtcctgctccttgtgatggtttacaaacgtaaaaagtctctttaatacctgtttttgcttcatattgttcagcgacagcttgctgtacggcaggcaccagctcttccgggatcagcgcgacgatacagccgccaaatccgccgccggtcatgcgtacgccacctttgtcgccaatcacagctttgacgatttctaccagagtgtcaatttgcggcacggtgatttcgaaatcatcgcgcatagaggcatgagactccgccatcaactcgcccatacgtttcaggtcgccttgctccagcgcgctggcagcttcaacggtgcgggcgttttcagtcagtatatgacgcacgcgttttgccacgatcgggtccagttcatgcgcaacagcgttgaactcttcaatggtgacatcacgcagggctggctgctggaagaaacgcgcaccggtttcgcactgttcacgacgggtgttgtattcgctgccaaccagggtacgtttgaagttactgttgatgatgacgacagccacacctttgggcatggaaactgctttggtccccagtgagcggcaatcgatcagcaaggcatgatctttcttgccgagcgcggaaattagctgatccatgatcccgcagttacagcctacaaactggttttctgcttcctgaccgttaagcgcgatttgtgcgccgtccagcggcagatgataaagctgctgcaatacggttccgaccgcgacttccagtgaagcggaagaacttaacccggcaccctgcggcacattgccgctgatcaccatgtccacgccgccgaagctgttgttacgcagttgcagatgtttcaccacgccacgaacgtagttagcccattgatagttttcatgtgcgacaatgggcgcatcgagggaaaactcgtcgagctgattttcataatcggctgccatcacgcgaactttacggtcatcgcgtggtgcacaactgatcacggtttgataatcaatcgcgcagggcagaacgaaaccgtcgttgtagtcggtgtgttcaccaatcaaattcacgcggccaggcgcctgaatggtgtgagtggcagggtagccaaatgcgttggcaaacagagattgtgttttttctttcagactcatgattgcctcctgggttagttcctcaccttgtcgtattatactatgccgatatactatgccgatgattaattgtcaacctttaatgaattataaaacgaaaggctcagtcgaaagactgggcctttcgttttatgcgttgcgcttaatctttctgcgaattgagatgacgccactggctgggcgtcatcccggtttcccgggtaaacaccaccgaaaaatagttactatcttcaaagccacattcggt…
encoding: naseq.html

https://synbiohub.org/public/SEGA/SEGA015_seq/1
type: Sequence
persistent identity: SEGA015 seq
display id: SEGA015_seq
version: 1
owned by: carbay
owned by: morno
elements: gtgcatggtctgcaggcgctgccgagccgtattgagcagatgctgtctcaggacaaacgcattgaagcgctggcagaagatttctctgacaaacatcacgcgctgttcctgggccgtggcgatcagtacccaatcgcgctggaaggcgcattgaagttgaaagagatctcttacattcacgctgaagcctacgctgctggcgaactgaaacacggtccgctggcgctaattgatgccgatatgccggttattgttgttgcaccgaacaacgaattgctggaaaaactgaaatccaacattgaagaagttcgcgcgcgtggcggtcagttgtatgtcttcgccgatcaggatgcgggttttgtaagtagcgataacatgcacatcatcgagatgccgcatgtggaagaggtgattgcaccgatcttctacaccgttccgctgcagctgctggcttaccatgtcgcgctgatcaaaggcaccgacgttgaccagccgcgtaacctggcaaaatcggttacggttgagtaataaatggatctcggtaccaaattccagaaaagagacgctttcgagcgtcttttttcgttttggtccgcgttgcgcttgacggctagctcagtcctaggtacagtgctagctactagagattaaagaggagaaaaactacatggaaaacctgtacttccagtttcgtagcaaaggagaagaacttgtcactggagttagcaaaggagaagaacttttcactggagttgtgccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgctacatacggaaaactcacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactctgacctatggcgttcaatgcttttcccgttatccggatcacatgaaacggcatgactttttcaagagtgccatgcccgaaggttatgtacaggaacgcactatatctttcaaagatgacgggaactacaagacgcgtgctgaagtcaagtttgaaggtgatacccttgttaatcgtatcgagttaaagggtattgattttaaagaagatggaaacattctcggacacaaactagagtacaactataactcacacaatgtatacatcacggcagacaaacaaaagaatggaatcaaagctaacttcaaaattcgccacaacattgaagatggttccgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtcgacacaatctgccctttcgaaagatcccaacgaaaagcgtgaccacatggtccttcttgagtttgtaactgctgctgggattacacatggcatggatgagctctacaaatgaccaattattgaaggccgctaacgcggcctttttttgtttctggtctcccaaaaatttatttgcttattaatcatccggctcgtataatgtgtggagggcccaagttcacttaaaaaggagaatagttatgaaatcgaacaatgcgctcatcgtcatcctcggcaccgtcaccctggatgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagcatcgccagtcactatggcgtgctgctagcgctatatgcgttgatgcaatttctatgcgcacccgttctcggagcactgtccgaccgctttggccgccgcccagtcctgctcgcttcgctacttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggattctctacgccggacgcatcgtggccggcatcaccggcgccacaggtgcggttgctggcgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtg…
encoding: naseq.html

https://synbiohub.org/public/SEGA/SEGA084_seq/1
type: Sequence
persistent identity: SEGA084 seq
display id: SEGA084_seq
version: 1
owned by: carbay
owned by: morno
elements: gtgcatggtctgcaggcgctgccgagccgtattgagcagatgctgtctcaggacaaacgcattgaagcgctggcagaagatttctctgacaaacatcacgcgctgttcctgggccgtggcgatcagtacccaatcgcgctggaaggcgcattgaagttgaaagagatctcttacattcacgctgaagcctacgctgctggcgaactgaaacacggtccgctggcgctaattgatgccgatatgccggttattgttgttgcaccgaacaacgaattgctggaaaaactgaaatccaacattgaagaagttcgcgcgcgtggcggtcagttgtatgtcttcgccgatcaggatgcgggttttgtaagtagcgataacatgcacatcatcgagatgccgcatgtggaagaggtgattgcaccgatcttctacaccgttccgctgcagctgctggcttaccatgtcgcgctgatcaaaggcaccgacgttgaccagccgcgtaacctggcaaaatcggttacggttgagtaataaatggatctcggtaccaaattccagaaaagagacgctttcgagcgtcttttttcgttttggtccaccgtattcatcagcactgtcctgctccttgtgatggtttacaaacgtaaaaagtctctttaatacctgtttttgcttcatattgttcagcgacagcttgctgtacggcaggcaccagctcttccgggatcagcgcgacgatacagccgccaaatccgccgccggtcatgcgtacgccacctttgtcgccaatcacagctttgacgatttctaccagagtgtcaatttgcggcacggtgatttcgaaatcatcgcgcatagaggcatgagactccgccatcaactcgcccatacgtttcaggtcgccttgctccagcgcgctggcagcttcaacggtgcgggcgttttcagtcagtatatgacgcacgcgttttgccacgatcgggtccagttcatgcgcaacagcgttgaactcttcaatggtgacatcacgcagggctggctgctggaagaaacgcgcaccggtttcgcactgttcacgacgggtgttgtattcgctgccaaccagggtacgtttgaagttactgttgatgatgacgacagccacacctttgggcatggaaactgctttggtccccagtgagcggcaatcgatcagcaaggcatgatctttcttgccgagcgcggaaattagctgatccatgatcccgcagttacagcctacaaactggttttctgcttcctgaccgttaagcgcgatttgtgcgccgtccagcggcagatgataaagctgctgcaatacggttccgaccgcgacttccagtgaagcggaagaacttaacccggcaccctgcggcacattgccgctgatcaccatgtccacgccgccgaagctgttgttacgcagttgcagatgtttcaccacgccacgaacgtagttagcccattgatagttttcatgtgcgacaatgggcgcatcgagggaaaactcgtcgagctgattttcataatcggctgccatcacgcgaactttacggtcatcgcgtggtgcacaactgatcacggtttgataatcaatcgcgcagggcagaacgaaaccgtcgttgtagtcggtgtgttcaccaatcaaattcacgcggccaggcgcctgaatggtgtgagtggcagggtagccaaatgcgttggcaaacagagattgtgttttttctttcagactcatgattgcctcctgggttagttcctcaccttgtcgtattatactatgccgatatactatgccgatgattaattgtcaacctttaatgaattataaaacgaaaggctcagtcgaaagactgggcctttcgttttatgcgttgcgcttaatctttctgcgaattgagatgacgccactggctgggcgtcatcccggtttcccgggtaaacaccaccgaaaaatagttactatcttcaaagccacattcggt…
encoding: naseq.html

https://synbiohub.org/public/SEGA/SEGA033_seq/1
type: Sequence
persistent identity: SEGA033 seq
display id: SEGA033_seq
version: 1
owned by: carbay
owned by: morno
elements: gtgcatggtctgcaggcgctgccgagccgtattgagcagatgctgtctcaggacaaacgcattgaagcgctggcagaagatttctctgacaaacatcacgcgctgttcctgggccgtggcgatcagtacccaatcgcgctggaaggcgcattgaagttgaaagagatctcttacattcacgctgaagcctacgctgctggcgaactgaaacacggtccgctggcgctaattgatgccgatatgccggttattgttgttgcaccgaacaacgaattgctggaaaaactgaaatccaacattgaagaagttcgcgcgcgtggcggtcagttgtatgtcttcgccgatcaggatgcgggttttgtaagtagcgataacatgcacatcatcgagatgccgcatgtggaagaggtgattgcaccgatcttctacaccgttccgctgcagctgctggcttaccatgtcgcgctgatcaaaggcaccgacgttgaccagccgcgtaacctggcaaaatcggttacggttgagtaataaatggatctcggtaccaaattccagaaaagagacgctttcgagcgtcttttttcgttttggtccgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgccagggtggtttttcttttcaccagtgagacgggcaacagctgattgcccttcaccgcctggccctgagagagttgcagcaagcggtccacgctggtttgccccagcaggcgaaaatcctgtttgatggtggttaacggcgggatataacatgagctgtcttcggtatcgtcgtatcccactaccgagatgtccgcaccaacgcgcagcccggactcggtaatggcgcgcattgcgcccagcgccatctgatcgttggcaaccagcatcgcagtgggaacgatgccctcattcagcatttgcatggtttgttgaaaaccggacatggcactccagtcgccttcccgttccgctatcggctgaatttgattgcgagtgagatatttatgccagccagccagacgcagacgcgccgagacagaacttaatgggcccgctaacagcgcgatttgctggtgacccaatgcgaccagatgctccacgcccagtcgcgtaccgtcttcatgggagaaaataatactgttgatgggtgtctggtcagagacatcaagaaataacgccggaacattagtgcaggcagcttccacagcaatggcatcctggtcatccagcggatagttaatgatcagcccactgacgcgttgcgcgagaagattgtgcaccgccgctttacaggcttcgacgccgcttcgttctaccatcgacaccaccacgctggcacccagttgatcggcgcgagatttaatcgccgcgacaatttgcgacggcgcgtgcagggccagactggaggtggcaacgccaatcagcaacgactgtttgcccgccagttgttgtgccacgcggttgggaatgtaattcagctccgccatcgccgcttccactttttcccgcgttttcgcagaaacgtggctggcctggttcaccacgcgggaaacggtctgataagagacaccggcatactctgcgacatcgtataacgttactggtttcacattcaccaccctgaattgactctcttccgggcgctatcatgccataccgcgaaaggttttgcgccattcgatggtgtcggatcgagatcgatctcgatcccgcgaaatttgacaattaatcatccggctcgtataatggaattgtgagcggataacaattcctactagagattaaagaggagaaatcaatgatgaagtacctgctgccgaccgctgctgctggtctgctgctcctcgctgcccagccggcgatggcccgtaaaggcgaagagctgttcactggtgtcgtccctattctggtggaactggatggt…
encoding: naseq.html

https://synbiohub.org/public/SEGA/SEGA045_seq/1
type: Sequence
persistent identity: SEGA045 seq
display id: SEGA045_seq
version: 1
owned by: carbay
owned by: morno
elements: gtgcatggtctgcaggcgctgccgagccgtattgagcagatgctgtctcaggacaaacgcattgaagcgctggcagaagatttctctgacaaacatcacgcgctgttcctgggccgtggcgatcagtacccaatcgcgctggaaggcgcattgaagttgaaagagatctcttacattcacgctgaagcctacgctgctggcgaactgaaacacggtccgctggcgctaattgatgccgatatgccggttattgttgttgcaccgaacaacgaattgctggaaaaactgaaatccaacattgaagaagttcgcgcgcgtggcggtcagttgtatgtcttcgccgatcaggatgcgggttttgtaagtagcgataacatgcacatcatcgagatgccgcatgtggaagaggtgattgcaccgatcttctacaccgttccgctgcagctgctggcttaccatgtcgcgctgatcaaaggcaccgacgttgaccagccgcgtaacctggcaaaatcggttacggttgagtaataaatggatctcggtaccaaattccagaaaagagacgctttcgagcgtcttttttcgttttggtccgcgttgcgcttaatctttctgcgaattgagatgacgccactggctgggcgtcatcccggtttcccgggtaaacaccaccgaaaaatagttactatcttcaaagccacattcggtcgaaatatcactgattaacaggcggctatgctggagaagatattgcgcatgacacactctgacctgtcgcagatattgattgatggtcattccagtctgctggcgaaattgctgacgcaaaacgcgctcactgcacgatgcctcatcacaaaatttatccagcgcaaagggacttttcaggctagccgccagccgggtaatcagcttatccagcaacgtttcgctggatgttggcggcaacgaatcactggtgtaacgatggcgattcagcaacatcaccaactgcccgaacagcaactcagccatttcgttagcaaacggcacatgctgactactttcatgctcaagctgaccaataacctgccgcgcctgcgccatccccatgctacctaagcgccagtgtggttgccctgcgctggcgttaaatcccggaatcgccccctgccagtcaagattcagcttcagacgctccgggcaataaataatattctgcaaaaccagatcgttaacggaagcgtaggagtgtttatcatcagcatgaatgtaaaagagatcgccacgggtaatgcgataagggcgatcgttgagtacatgcaggccattaccgcgccagacaatcaccagctcacaaaaatcatgtgtatgttcagcaaagacatcttgcggataacggtcagccacagcgactgcctgctggtcgctggcaaaaaaatcatctttgagaagttttaactgatgcgccaccgtggctacctcggccagagaacgaagttgattattcgcaatatggcgtacaaatacgttgagaagattcgcgttattgcagaaagccatcccgtccctggcgaatatcacgcggtgaccagttaaactctcggcgaaaaagcgtcgaaaagtggttactgtcgctgaatccacagcgataggcgatgtcagtaacgctggcctcgctgtggcgtagcagatgtcgggctttcatcagtcgcaggcggttcaggtatcgctgaggcgtcagtcccgtttgctgcttaagctgccgatgtagcgtacgcagtgaaagagaaaattgatccgccacggcatcccaattcacctcatcggcaaaatggtcctccagccaggccagaagcaagttgagacgtgatgcgctgttttccaggttctcctgcaaactgcttttacgcagcaagagcagtaattgcataaacaagatctcgcgactggcggtcgagggtaaatcattttccccttcctgctgttccatctgtgcaaccagctgtcgcacctgctgc…
encoding: naseq.html

https://synbiohub.org/public/SEGA/SEGA103_seq/1
type: Sequence
persistent identity: SEGA103 seq
display id: SEGA103_seq
version: 1
owned by: carbay
owned by: morno
elements: gtgcatggtctgcaggcgctgccgagccgtattgagcagatgctgtctcaggacaaacgcattgaagcgctggcagaagatttctctgacaaacatcacgcgctgttcctgggccgtggcgatcagtacccaatcgcgctggaaggcgcattgaagttgaaagagatctcttacattcacgctgaagcctacgctgctggcgaactgaaacacggtccgctggcgctaattgatgccgatatgccggttattgttgttgcaccgaacaacgaattgctggaaaaactgaaatccaacattgaagaagttcgcgcgcgtggcggtcagttgtatgtcttcgccgatcaggatgcgggttttgtaagtagcgataacatgcacatcatcgagatgccgcatgtggaagaggtgattgcaccgatcttctacaccgttccgctgcagctgctggcttaccatgtcgcgctgatcaaaggcaccgacgttgaccagccgcgtaacctggcaaaatcggttacggttgagtaataaatggatctcggtaccaaattccagaaaagagacgctttcgagcgtcttttttcgttttggtccgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgccagggtggtttttcttttcaccagtgagacgggcaacagctgattgcccttcaccgcctggccctgagagagttgcagcaagcggtccacgctggtttgccccagcaggcgaaaatcctgtttgatggtggttaacggcgggatataacatgagctgtcttcggtatcgtcgtatcccactaccgagatgtccgcaccaacgcgcagcccggactcggtaatggcgcgcattgcgcccagcgccatctgatcgttggcaaccagcatcgcagtgggaacgatgccctcattcagcatttgcatggtttgttgaaaaccggacatggcactccagtcgccttcccgttccgctatcggctgaatttgattgcgagtgagatatttatgccagccagccagacgcagacgcgccgagacagaacttaatgggcccgctaacagcgcgatttgctggtgacccaatgcgaccagatgctccacgcccagtcgcgtaccgtcttcatgggagaaaataatactgttgatgggtgtctggtcagagacatcaagaaataacgccggaacattagtgcaggcagcttccacagcaatggcatcctggtcatccagcggatagttaatgatcagcccactgacgcgttgcgcgagaagattgtgcaccgccgctttacaggcttcgacgccgcttcgttctaccatcgacaccaccacgctggcacccagttgatcggcgcgagatttaatcgccgcgacaatttgcgacggcgcgtgcagggccagactggaggtggcaacgccaatcagcaacgactgtttgcccgccagttgttgtgccacgcggttgggaatgtaattcagctccgccatcgccgcttccactttttcccgcgttttcgcagaaacgtggctggcctggttcaccacgcgggaaacggtctgataagagacaccggcatactctgcgacatcgtataacgttactggtttcacattcaccaccctgaattgactctcttccgggcgctatcatgccataccgcgaaaggttttgcgccattcgatggtgtcggatcgagatcgatctcgatcccgcgaaattaatacgactcactataggggaattgtgagcggataacaattcctactagagattaaagaggagaaaggggggatgaaggctattttcgtactgaaacatcttaatcatgctaaggaggttttctaatggtactgccgggcctcttgcgggatatcgtccattccgacagcatcgccagtcactatggcgtgctgctagcgctata…
encoding: naseq.html

https://synbiohub.org/public/SEGA/SEGA039_seq/1
type: Sequence
persistent identity: SEGA039 seq
display id: SEGA039_seq
version: 1
owned by: carbay
owned by: morno
elements: gtgcatggtctgcaggcgctgccgagccgtattgagcagatgctgtctcaggacaaacgcattgaagcgctggcagaagatttctctgacaaacatcacgcgctgttcctgggccgtggcgatcagtacccaatcgcgctggaaggcgcattgaagttgaaagagatctcttacattcacgctgaagcctacgctgctggcgaactgaaacacggtccgctggcgctaattgatgccgatatgccggttattgttgttgcaccgaacaacgaattgctggaaaaactgaaatccaacattgaagaagttcgcgcgcgtggcggtcagttgtatgtcttcgccgatcaggatgcgggttttgtaagtagcgataacatgcacatcatcgagatgccgcatgtggaagaggtgattgcaccgatcttctacaccgttccgctgcagctgctggcttaccatgtcgcgctgatcaaaggcaccgacgttgaccagccgcgtaacctggcaaaatcggttacggttgagtaataaatggatctcggtaccaaattccagaaaagagacgctttcgagcgtcttttttcgttttggtccgcgttgcgcttaatctttctgcgaattgagatgacgccactggctgggcgtcatcccggtttcccgggtaaacaccaccgaaaaatagttactatcttcaaagccacattcggtcgaaatatcactgattaacaggcggctatgctggagaagatattgcgcatgacacactctgacctgtcgcagatattgattgatggtcattccagtctgctggcgaaattgctgacgcaaaacgcgctcactgcacgatgcctcatcacaaaatttatccagcgcaaagggacttttcaggctagccgccagccgggtaatcagcttatccagcaacgtttcgctggatgttggcggcaacgaatcactggtgtaacgatggcgattcagcaacatcaccaactgcccgaacagcaactcagccatttcgttagcaaacggcacatgctgactactttcatgctcaagctgaccaataacctgccgcgcctgcgccatccccatgctacctaagcgccagtgtggttgccctgcgctggcgttaaatcccggaatcgccccctgccagtcaagattcagcttcagacgctccgggcaataaataatattctgcaaaaccagatcgttaacggaagcgtaggagtgtttatcatcagcatgaatgtaaaagagatcgccacgggtaatgcgataagggcgatcgttgagtacatgcaggccattaccgcgccagacaatcaccagctcacaaaaatcatgtgtatgttcagcaaagacatcttgcggataacggtcagccacagcgactgcctgctggtcgctggcaaaaaaatcatctttgagaagttttaactgatgcgccaccgtggctacctcggccagagaacgaagttgattattcgcaatatggcgtacaaatacgttgagaagattcgcgttattgcagaaagccatcccgtccctggcgaatatcacgcggtgaccagttaaactctcggcgaaaaagcgtcgaaaagtggttactgtcgctgaatccacagcgataggcgatgtcagtaacgctggcctcgctgtggcgtagcagatgtcgggctttcatcagtcgcaggcggttcaggtatcgctgaggcgtcagtcccgtttgctgcttaagctgccgatgtagcgtacgcagtgaaagagaaaattgatccgccacggcatcccaattcacctcatcggcaaaatggtcctccagccaggccagaagcaagttgagacgtgatgcgctgttttccaggttctcctgcaaactgcttttacgcagcaagagcagtaattgcataaacaagatctcgcgactggcggtcgagggtaaatcattttccccttcctgctgttccatctgtgcaaccagctgtcgcacctgctgc…
encoding: naseq.html

https://synbiohub.org/public/SEGA/SEGA008_seq/1
type: Sequence
persistent identity: SEGA008 seq
display id: SEGA008_seq
version: 1
owned by: carbay
owned by: morno
elements: gtgcatggtctgcaggcgctgccgagccgtattgagcagatgctgtctcaggacaaacgcattgaagcgctggcagaagatttctctgacaaacatcacgcgctgttcctgggccgtggcgatcagtacccaatcgcgctggaaggcgcattgaagttgaaagagatctcttacattcacgctgaagcctacgctgctggcgaactgaaacacggtccgctggcgctaattgatgccgatatgccggttattgttgttgcaccgaacaacgaattgctggaaaaactgaaatccaacattgaagaagttcgcgcgcgtggcggtcagttgtatgtcttcgccgatcaggatgcgggttttgtaagtagcgataacatgcacatcatcgagatgccgcatgtggaagaggtgattgcaccgatcttctacaccgttccgctgcagctgctggcttaccatgtcgcgctgatcaaaggcaccgacgttgaccagccgcgtaacctggcaaaatcggttacggttgagtaataaatggatctcggtaccaaattccagaaaagagacgctttcgagcgtcttttttcgttttggtccgcgttgcgcttgacggctagctcagtcctaggtacagtgctagctactagagattaaagaggagaaaggggggatgcaaatattcgtgaaaacactgaccggcaaaaccattaccctggaggtggagcctggcagcgcccatcatcatcaccatcaccatcaccatcatgctggtagcagcgatacgatcgaaaacgtcaaagccaaaattcaggacaaagagggcattccgcctgatcaacaacgtctgatcttcgccggaaaacagctggaggatggccgtacactgagtgactataacattcaagagaaatcgacactggaactggttctggagctgcagaatgaaacccagtcagcaacttctgacgcttctggcggtagcaaaggagaagaacttttcactggagttgtgccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgctacatacggaaaactcacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactctgacctatggcgttcaatgcttttcccgttatccggatcacatgaaacggcatgactttttcaagagtgccatgcccgaaggttatgtacaggaacgcactatatctttcaaagatgacgggaactacaagacgcgtgctgaagtcaagtttgaaggtgatacccttgttaatcgtatcgagttaaagggtattgattttaaagaagatggaaacattctcggacacaaactagagtacaactataactcacacaatgtatacatcacggcagacaaacaaaagaatggaatcaaagctaacttcaaaattcgccacaacattgaagatggttccgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtcgacacaatctgccctttcgaaagatcccaacgaaaagcgtgaccacatggtccttcttgagtttgtaactgctgctgggattacacatggcatggatgagctctacaaatgaccaattattgaaggccgctaacgcggcctttttttgtttctggtctcccaaaaatttatttgcttattaatcatccggctcgtataatgtgtggagggcccaagttcacttaaaaaggagaatagttatgaaatcgaacaatgcgctcatcgtcatcctcggcaccgtcaccctggatgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagcatcgccagtcactatggcgtgctgctagcgctatatgcgttgatgcaatttctatgcgcaccc…
encoding: naseq.html

https://synbiohub.org/public/SEGA/SEGA047_seq/1
type: Sequence
persistent identity: SEGA047 seq
display id: SEGA047_seq
version: 1
owned by: carbay
owned by: morno
elements: gtgcatggtctgcaggcgctgccgagccgtattgagcagatgctgtctcaggacaaacgcattgaagcgctggcagaagatttctctgacaaacatcacgcgctgttcctgggccgtggcgatcagtacccaatcgcgctggaaggcgcattgaagttgaaagagatctcttacattcacgctgaagcctacgctgctggcgaactgaaacacggtccgctggcgctaattgatgccgatatgccggttattgttgttgcaccgaacaacgaattgctggaaaaactgaaatccaacattgaagaagttcgcgcgcgtggcggtcagttgtatgtcttcgccgatcaggatgcgggttttgtaagtagcgataacatgcacatcatcgagatgccgcatgtggaagaggtgattgcaccgatcttctacaccgttccgctgcagctgctggcttaccatgtcgcgctgatcaaaggcaccgacgttgaccagccgcgtaacctggcaaaatcggttacggttgagtaataaatggatctcggtaccaaattccagaaaagagacgctttcgagcgtcttttttcgttttggtccgcgttgcgcttaatctttctgcgaattgagatgacgccactggctgggcgtcatcccggtttcccgggtaaacaccaccgaaaaatagttactatcttcaaagccacattcggtcgaaatatcactgattaacaggcggctatgctggagaagatattgcgcatgacacactctgacctgtcgcagatattgattgatggtcattccagtctgctggcgaaattgctgacgcaaaacgcgctcactgcacgatgcctcatcacaaaatttatccagcgcaaagggacttttcaggctagccgccagccgggtaatcagcttatccagcaacgtttcgctggatgttggcggcaacgaatcactggtgtaacgatggcgattcagcaacatcaccaactgcccgaacagcaactcagccatttcgttagcaaacggcacatgctgactactttcatgctcaagctgaccaataacctgccgcgcctgcgccatccccatgctacctaagcgccagtgtggttgccctgcgctggcgttaaatcccggaatcgccccctgccagtcaagattcagcttcagacgctccgggcaataaataatattctgcaaaaccagatcgttaacggaagcgtaggagtgtttatcatcagcatgaatgtaaaagagatcgccacgggtaatgcgataagggcgatcgttgagtacatgcaggccattaccgcgccagacaatcaccagctcacaaaaatcatgtgtatgttcagcaaagacatcttgcggataacggtcagccacagcgactgcctgctggtcgctggcaaaaaaatcatctttgagaagttttaactgatgcgccaccgtggctacctcggccagagaacgaagttgattattcgcaatatggcgtacaaatacgttgagaagattcgcgttattgcagaaagccatcccgtccctggcgaatatcacgcggtgaccagttaaactctcggcgaaaaagcgtcgaaaagtggttactgtcgctgaatccacagcgataggcgatgtcagtaacgctggcctcgctgtggcgtagcagatgtcgggctttcatcagtcgcaggcggttcaggtatcgctgaggcgtcagtcccgtttgctgcttaagctgccgatgtagcgtacgcagtgaaagagaaaattgatccgccacggcatcccaattcacctcatcggcaaaatggtcctccagccaggccagaagcaagttgagacgtgatgcgctgttttccaggttctcctgcaaactgcttttacgcagcaagagcagtaattgcataaacaagatctcgcgactggcggtcgagggtaaatcattttccccttcctgctgttccatctgtgcaaccagctgtcgcacctgctgc…
encoding: naseq.html

https://synbiohub.org/public/SEGA/SEGA043_seq/1
type: Sequence
persistent identity: SEGA043 seq
display id: SEGA043_seq
version: 1
owned by: carbay
owned by: morno
elements: gtgcatggtctgcaggcgctgccgagccgtattgagcagatgctgtctcaggacaaacgcattgaagcgctggcagaagatttctctgacaaacatcacgcgctgttcctgggccgtggcgatcagtacccaatcgcgctggaaggcgcattgaagttgaaagagatctcttacattcacgctgaagcctacgctgctggcgaactgaaacacggtccgctggcgctaattgatgccgatatgccggttattgttgttgcaccgaacaacgaattgctggaaaaactgaaatccaacattgaagaagttcgcgcgcgtggcggtcagttgtatgtcttcgccgatcaggatgcgggttttgtaagtagcgataacatgcacatcatcgagatgccgcatgtggaagaggtgattgcaccgatcttctacaccgttccgctgcagctgctggcttaccatgtcgcgctgatcaaaggcaccgacgttgaccagccgcgtaacctggcaaaatcggttacggttgagtaataaatggatctcggtaccaaattccagaaaagagacgctttcgagcgtcttttttcgttttggtccgcgttgcgcttaatctttctgcgaattgagatgacgccactggctgggcgtcatcccggtttcccgggtaaacaccaccgaaaaatagttactatcttcaaagccacattcggtcgaaatatcactgattaacaggcggctatgctggagaagatattgcgcatgacacactctgacctgtcgcagatattgattgatggtcattccagtctgctggcgaaattgctgacgcaaaacgcgctcactgcacgatgcctcatcacaaaatttatccagcgcaaagggacttttcaggctagccgccagccgggtaatcagcttatccagcaacgtttcgctggatgttggcggcaacgaatcactggtgtaacgatggcgattcagcaacatcaccaactgcccgaacagcaactcagccatttcgttagcaaacggcacatgctgactactttcatgctcaagctgaccaataacctgccgcgcctgcgccatccccatgctacctaagcgccagtgtggttgccctgcgctggcgttaaatcccggaatcgccccctgccagtcaagattcagcttcagacgctccgggcaataaataatattctgcaaaaccagatcgttaacggaagcgtaggagtgtttatcatcagcatgaatgtaaaagagatcgccacgggtaatgcgataagggcgatcgttgagtacatgcaggccattaccgcgccagacaatcaccagctcacaaaaatcatgtgtatgttcagcaaagacatcttgcggataacggtcagccacagcgactgcctgctggtcgctggcaaaaaaatcatctttgagaagttttaactgatgcgccaccgtggctacctcggccagagaacgaagttgattattcgcaatatggcgtacaaatacgttgagaagattcgcgttattgcagaaagccatcccgtccctggcgaatatcacgcggtgaccagttaaactctcggcgaaaaagcgtcgaaaagtggttactgtcgctgaatccacagcgataggcgatgtcagtaacgctggcctcgctgtggcgtagcagatgtcgggctttcatcagtcgcaggcggttcaggtatcgctgaggcgtcagtcccgtttgctgcttaagctgccgatgtagcgtacgcagtgaaagagaaaattgatccgccacggcatcccaattcacctcatcggcaaaatggtcctccagccaggccagaagcaagttgagacgtgatgcgctgttttccaggttctcctgcaaactgcttttacgcagcaagagcagtaattgcataaacaagatctcgcgactggcggtcgagggtaaatcattttccccttcctgctgttccatctgtgcaaccagctgtcgcacctgctgc…
encoding: naseq.html

https://synbiohub.org/public/SEGA/SEGA049_seq/1
type: Sequence
persistent identity: SEGA049 seq
display id: SEGA049_seq
version: 1
owned by: carbay
owned by: morno
elements: gtgcatggtctgcaggcgctgccgagccgtattgagcagatgctgtctcaggacaaacgcattgaagcgctggcagaagatttctctgacaaacatcacgcgctgttcctgggccgtggcgatcagtacccaatcgcgctggaaggcgcattgaagttgaaagagatctcttacattcacgctgaagcctacgctgctggcgaactgaaacacggtccgctggcgctaattgatgccgatatgccggttattgttgttgcaccgaacaacgaattgctggaaaaactgaaatccaacattgaagaagttcgcgcgcgtggcggtcagttgtatgtcttcgccgatcaggatgcgggttttgtaagtagcgataacatgcacatcatcgagatgccgcatgtggaagaggtgattgcaccgatcttctacaccgttccgctgcagctgctggcttaccatgtcgcgctgatcaaaggcaccgacgttgaccagccgcgtaacctggcaaaatcggttacggttgagtaataaatggatctcggtaccaaattccagaaaagagacgctttcgagcgtcttttttcgttttggtccgcgttgcgcttaatctttctgcgaattgagatgacgccactggctgggcgtcatcccggtttcccgggtaaacaccaccgaaaaatagttactatcttcaaagccacattcggtcgaaatatcactgattaacaggcggctatgctggagaagatattgcgcatgacacactctgacctgtcgcagatattgattgatggtcattccagtctgctggcgaaattgctgacgcaaaacgcgctcactgcacgatgcctcatcacaaaatttatccagcgcaaagggacttttcaggctagccgccagccgggtaatcagcttatccagcaacgtttcgctggatgttggcggcaacgaatcactggtgtaacgatggcgattcagcaacatcaccaactgcccgaacagcaactcagccatttcgttagcaaacggcacatgctgactactttcatgctcaagctgaccaataacctgccgcgcctgcgccatccccatgctacctaagcgccagtgtggttgccctgcgctggcgttaaatcccggaatcgccccctgccagtcaagattcagcttcagacgctccgggcaataaataatattctgcaaaaccagatcgttaacggaagcgtaggagtgtttatcatcagcatgaatgtaaaagagatcgccacgggtaatgcgataagggcgatcgttgagtacatgcaggccattaccgcgccagacaatcaccagctcacaaaaatcatgtgtatgttcagcaaagacatcttgcggataacggtcagccacagcgactgcctgctggtcgctggcaaaaaaatcatctttgagaagttttaactgatgcgccaccgtggctacctcggccagagaacgaagttgattattcgcaatatggcgtacaaatacgttgagaagattcgcgttattgcagaaagccatcccgtccctggcgaatatcacgcggtgaccagttaaactctcggcgaaaaagcgtcgaaaagtggttactgtcgctgaatccacagcgataggcgatgtcagtaacgctggcctcgctgtggcgtagcagatgtcgggctttcatcagtcgcaggcggttcaggtatcgctgaggcgtcagtcccgtttgctgcttaagctgccgatgtagcgtacgcagtgaaagagaaaattgatccgccacggcatcccaattcacctcatcggcaaaatggtcctccagccaggccagaagcaagttgagacgtgatgcgctgttttccaggttctcctgcaaactgcttttacgcagcaagagcagtaattgcataaacaagatctcgcgactggcggtcgagggtaaatcattttccccttcctgctgttccatctgtgcaaccagctgtcgcacctgctgc…
encoding: naseq.html

https://synbiohub.org/public/SEGA/SEGA063_seq/1
type: Sequence
persistent identity: SEGA063 seq
display id: SEGA063_seq
version: 1
owned by: carbay
owned by: morno
elements: gtgcatggtctgcaggcgctgccgagccgtattgagcagatgctgtctcaggacaaacgcattgaagcgctggcagaagatttctctgacaaacatcacgcgctgttcctgggccgtggcgatcagtacccaatcgcgctggaaggcgcattgaagttgaaagagatctcttacattcacgctgaagcctacgctgctggcgaactgaaacacggtccgctggcgctaattgatgccgatatgccggttattgttgttgcaccgaacaacgaattgctggaaaaactgaaatccaacattgaagaagttcgcgcgcgtggcggtcagttgtatgtcttcgccgatcaggatgcgggttttgtaagtagcgataacatgcacatcatcgagatgccgcatgtggaagaggtgattgcaccgatcttctacaccgttccgctgcagctgctggcttaccatgtcgcgctgatcaaaggcaccgacgttgaccagccgcgtaacctggcaaaatcggttacggttgagtaataaatggatctcggtaccaaattccagaaaagagacgctttcgagcgtcttttttcgttttggtccgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgccagggtggtttttcttttcaccagtgagacgggcaacagctgattgcccttcaccgcctggccctgagagagttgcagcaagcggtccacgctggtttgccccagcaggcgaaaatcctgtttgatggtggttaacggcgggatataacatgagctgtcttcggtatcgtcgtatcccactaccgagatgtccgcaccaacgcgcagcccggactcggtaatggcgcgcattgcgcccagcgccatctgatcgttggcaaccagcatcgcagtgggaacgatgccctcattcagcatttgcatggtttgttgaaaaccggacatggcactccagtcgccttcccgttccgctatcggctgaatttgattgcgagtgagatatttatgccagccagccagacgcagacgcgccgagacagaacttaatgggcccgctaacagcgcgatttgctggtgacccaatgcgaccagatgctccacgcccagtcgcgtaccgtcttcatgggagaaaataatactgttgatgggtgtctggtcagagacatcaagaaataacgccggaacattagtgcaggcagcttccacagcaatggcatcctggtcatccagcggatagttaatgatcagcccactgacgcgttgcgcgagaagattgtgcaccgccgctttacaggcttcgacgccgcttcgttctaccatcgacaccaccacgctggcacccagttgatcggcgcgagatttaatcgccgcgacaatttgcgacggcgcgtgcagggccagactggaggtggcaacgccaatcagcaacgactgtttgcccgccagttgttgtgccacgcggttgggaatgtaattcagctccgccatcgccgcttccactttttcccgcgttttcgcagaaacgtggctggcctggttcaccacgcgggaaacggtctgataagagacaccggcatactctgcgacatcgtataacgttactggtttcacattcaccaccctgaattgactctcttccgggcgctatcatgccataccgcgaaaggttttgcgccattcgatggtgtcggatcgagatcgatctcgatcccgcgaaattaatacgactcactataggggaattgtgagcggataacaattcctactagagattaaagaggagaaaaactacatggaaaacctgtacttccagagcaaaggagaagaacttttcactggagttgtgccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgctacat…
encoding: naseq.html

https://synbiohub.org/public/SEGA/SEGA077_seq/1
type: Sequence
persistent identity: SEGA077 seq
display id: SEGA077_seq
version: 1
owned by: carbay
owned by: morno
elements: gtgcatggtctgcaggcgctgccgagccgtattgagcagatgctgtctcaggacaaacgcattgaagcgctggcagaagatttctctgacaaacatcacgcgctgttcctgggccgtggcgatcagtacccaatcgcgctggaaggcgcattgaagttgaaagagatctcttacattcacgctgaagcctacgctgctggcgaactgaaacacggtccgctggcgctaattgatgccgatatgccggttattgttgttgcaccgaacaacgaattgctggaaaaactgaaatccaacattgaagaagttcgcgcgcgtggcggtcagttgtatgtcttcgccgatcaggatgcgggttttgtaagtagcgataacatgcacatcatcgagatgccgcatgtggaagaggtgattgcaccgatcttctacaccgttccgctgcagctgctggcttaccatgtcgcgctgatcaaaggcaccgacgttgaccagccgcgtaacctggcaaaatcggttacggttgagtaataaatggatctcggtaccaaattccagaaaagagacgctttcgagcgtcttttttcgttttggtccccaattaggatccttatcaggacccactttcacatttaagttgtttttctaatccgcatatgatcaattcaaggccgaataagaaggctggctctgcaccttggtgatcaaataattcgatagcttgtcgtaataatggcggcatactatcagtagtaggtgtttccctttcttctttagcgacttgatgctcttgatcttccaatacgcaacctaaagtaaaatgccccacagcgctgagtgcatataatgcattctctagtgaaaaaccttgttggcataaaaaggctaattgattttcgagagtttcatactgtttttctgtaggccgtgtacctaaatgtacttttgctccatcgcgatgacttagtaaagcacatctaaaacttttagcgttattacgtaaaaaatcttgccagctttccccttctaaagggcaaaagtgagtatggtgcctatctaacatctcaatggctaaggcgtcgagcaaagcccgcttattttttacatgccaatacaatgtaggctgctctacacctagcttctgggcgagtttacgggttgttaaaccttcgattccgacctcattaagcagctctaatgcgctgttaatcactttacttttatctaatctggacatattcaccaccctgaattgactctcttccgggcgctatcatgccataccgcgaaaggttttgcgccattcgatggcgcgccgccattggagcttaacgatcgttggctgttttcagcaggacgcactgacctccctatcagtgatagagattgacatccctatcagtgatagagatactgagcacagctgtcaccggatgtgctttccggtctgatgagtccgtgaggacgaaacagcctctacaaataattttgtttaatactagagattaaagaggagaaactatatatgaaagcaattttcgtactgaaacatcttaatcatgctaaggaggttttctaatgagcaaaggagaagaacttttcactggagttgtgccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgctacatacggaaaactcacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactctgacctatggcgttcaatgcttttcccgttatccggatcacatgaaacggcatgactttttcaagagtgccatgcccgaaggttatgtacaggaacgcactatatctttcaaagatgacgggaactacaagacgcgtgctgaagtcaagtttgaaggtgatacccttgttaatcgtatcgagttaaagggtattgattttaaagaagatggaaacattctcggacacaaactaga…
encoding: naseq.html

https://synbiohub.org/public/SEGA/SEGA076_seq/1
type: Sequence
persistent identity: SEGA076 seq
display id: SEGA076_seq
version: 1
owned by: carbay
owned by: morno
elements: gtgcatggtctgcaggcgctgccgagccgtattgagcagatgctgtctcaggacaaacgcattgaagcgctggcagaagatttctctgacaaacatcacgcgctgttcctgggccgtggcgatcagtacccaatcgcgctggaaggcgcattgaagttgaaagagatctcttacattcacgctgaagcctacgctgctggcgaactgaaacacggtccgctggcgctaattgatgccgatatgccggttattgttgttgcaccgaacaacgaattgctggaaaaactgaaatccaacattgaagaagttcgcgcgcgtggcggtcagttgtatgtcttcgccgatcaggatgcgggttttgtaagtagcgataacatgcacatcatcgagatgccgcatgtggaagaggtgattgcaccgatcttctacaccgttccgctgcagctgctggcttaccatgtcgcgctgatcaaaggcaccgacgttgaccagccgcgtaacctggcaaaatcggttacggttgagtaataaatggatctcggtaccaaattccagaaaagagacgctttcgagcgtcttttttcgttttggtccccaattaggatccttatcaggacccactttcacatttaagttgtttttctaatccgcatatgatcaattcaaggccgaataagaaggctggctctgcaccttggtgatcaaataattcgatagcttgtcgtaataatggcggcatactatcagtagtaggtgtttccctttcttctttagcgacttgatgctcttgatcttccaatacgcaacctaaagtaaaatgccccacagcgctgagtgcatataatgcattctctagtgaaaaaccttgttggcataaaaaggctaattgattttcgagagtttcatactgtttttctgtaggccgtgtacctaaatgtacttttgctccatcgcgatgacttagtaaagcacatctaaaacttttagcgttattacgtaaaaaatcttgccagctttccccttctaaagggcaaaagtgagtatggtgcctatctaacatctcaatggctaaggcgtcgagcaaagcccgcttattttttacatgccaatacaatgtaggctgctctacacctagcttctgggcgagtttacgggttgttaaaccttcgattccgacctcattaagcagctctaatgcgctgttaatcactttacttttatctaatctggacatattcaccaccctgaattgactctcttccgggcgctatcatgccataccgcgaaaggttttgcgccattcgatggcgcgccgccattggagcttaacgatcgttggctgttttcagcaggacgcactgacctccctatcagtgatagagattgacatccctatcagtgatagagatactgagcacagctgtcaccggatgtgctttccggtctgatgagtccgtgaggacgaaacagcctctacaaataattttgtttaatactagagattaaagaggagaaaggggggatgaaggctattttcgtactgaaacatcttaatcatgctaaggaggttttctaatgagcaaaggagaagaacttttcactggagttgtgccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgctacatacggaaaactcacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactctgacctatggcgttcaatgcttttcccgttatccggatcacatgaaacggcatgactttttcaagagtgccatgcccgaaggttatgtacaggaacgcactatatctttcaaagatgacgggaactacaagacgcgtgctgaagtcaagtttgaaggtgatacccttgttaatcgtatcgagttaaagggtattgattttaaagaagatggaaacattctcggacacaaactaga…
encoding: naseq.html

https://synbiohub.org/public/SEGA/SEGA018_seq/1
type: Sequence
persistent identity: SEGA018 seq
display id: SEGA018_seq
version: 1
owned by: carbay
owned by: morno
elements: gtgcatggtctgcaggcgctgccgagccgtattgagcagatgctgtctcaggacaaacgcattgaagcgctggcagaagatttctctgacaaacatcacgcgctgttcctgggccgtggcgatcagtacccaatcgcgctggaaggcgcattgaagttgaaagagatctcttacattcacgctgaagcctacgctgctggcgaactgaaacacggtccgctggcgctaattgatgccgatatgccggttattgttgttgcaccgaacaacgaattgctggaaaaactgaaatccaacattgaagaagttcgcgcgcgtggcggtcagttgtatgtcttcgccgatcaggatgcgggttttgtaagtagcgataacatgcacatcatcgagatgccgcatgtggaagaggtgattgcaccgatcttctacaccgttccgctgcagctgctggcttaccatgtcgcgctgatcaaaggcaccgacgttgaccagccgcgtaacctggcaaaatcggttacggttgagtaataaatggatctcggtaccaaattccagaaaagagacgctttcgagcgtcttttttcgttttggtccgcgttgcgcttgacggctagctcagtcctaggtacagtgctagctactagagattaaagaggagaaatcaatgatgaagtacctgctgccgaccgctgctgctggtctgctgctcctcgctgcccagccggcgatggcccgtaaaggcgaagagctgttcactggtgtcgtccctattctggtggaactggatggtgatgtcaacggtcataagttttccgtgcgtggcgagggtgaaggtgacgcaactaatggtaaactgacgctgaagttcatctgtactactggtaaactgccggtaccttggccgactctggtaacgacgctgacttatggtgttcagtgctttgctcgttatccggaccacatgaagcagcatgacttcttcaagtccgccatgccggaaggctatgtgcaggaacgcacgatttcctttaaggatgacggcacgtacaaaacgcgtgcggaagtgaaatttgaaggcgataccctggtaaaccgcattgagctgaaaggcattgactttaaagaagacggcaatatcctgggccataagctggaatacaattttaacagccacaatgtttacatcaccgccgataaacaaaaaaatggcattaaagcgaattttaaaattcgccacaacgtggaggatggcagcgtgcagctggctgatcactaccagcaaaacactccaatcggtgatggtcctgttctgctgccagacaatcactatctgagcacgcaaagcgttctgtctaaagatccgaacgagaaacgcgatcacatggttctgctggagttcgtaaccgcagcgggcatcacgcatggtatggatgaactgtacaaatgaccaattattgaaggccgctaacgcggcctttttttgtttctggtctcccaaaaatttatttgcttattaatcatccggctcgtataatgtgtggagggcccaagttcacttaaaaaggagaatagttatgaaatcgaacaatgcgctcatcgtcatcctcggcaccgtcaccctggatgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagcatcgccagtcactatggcgtgctgctagcgctatatgcgttgatgcaatttctatgcgcacccgttctcggagcactgtccgaccgctttggccgccgcccagtcctgctcgcttcgctacttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggattctctacgccggacgcatcgtggccggcatcaccggcgccacaggtgcggttgctggcgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtg…
encoding: naseq.html

https://synbiohub.org/public/SEGA/SEGA002_seq/1
type: Sequence
persistent identity: SEGA002 seq
display id: SEGA002_seq
version: 1
owned by: carbay
owned by: morno
elements: gtgcatggtctgcaggcgctgccgagccgtattgagcagatgctgtctcaggacaaacgcattgaagcgctggcagaagatttctctgacaaacatcacgcgctgttcctgggccgtggcgatcagtacccaatcgcgctggaaggcgcattgaagttgaaagagatctcttacattcacgctgaagcctacgctgctggcgaactgaaacacggtccgctggcgctaattgatgccgatatgccggttattgttgttgcaccgaacaacgaattgctggaaaaactgaaatccaacattgaagaagttcgcgcgcgtggcggtcagttgtatgtcttcgccgatcaggatgcgggttttgtaagtagcgataacatgcacatcatcgagatgccgcatgtggaagaggtgattgcaccgatcttctacaccgttccgctgcagctgctggcttaccatgtcgcgctgatcaaaggcaccgacgttgaccagccgcgtaacctggcaaaatcggttacggttgagtaataaatggatctcggtaccaaattccagaaaagagacgctttcgagcgtcttttttcgttttggtccgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgccagggtggtttttcttttcaccagtgagacgggcaacagctgattgcccttcaccgcctggccctgagagagttgcagcaagcggtccacgctggtttgccccagcaggcgaaaatcctgtttgatggtggttaacggcgggatataacatgagctgtcttcggtatcgtcgtatcccactaccgagatgtccgcaccaacgcgcagcccggactcggtaatggcgcgcattgcgcccagcgccatctgatcgttggcaaccagcatcgcagtgggaacgatgccctcattcagcatttgcatggtttgttgaaaaccggacatggcactccagtcgccttcccgttccgctatcggctgaatttgattgcgagtgagatatttatgccagccagccagacgcagacgcgccgagacagaacttaatgggcccgctaacagcgcgatttgctggtgacccaatgcgaccagatgctccacgcccagtcgcgtaccgtcttcatgggagaaaataatactgttgatgggtgtctggtcagagacatcaagaaataacgccggaacattagtgcaggcagcttccacagcaatggcatcctggtcatccagcggatagttaatgatcagcccactgacgcgttgcgcgagaagattgtgcaccgccgctttacaggcttcgacgccgcttcgttctaccatcgacaccaccacgctggcacccagttgatcggcgcgagatttaatcgccgcgacaatttgcgacggcgcgtgcagggccagactggaggtggcaacgccaatcagcaacgactgtttgcccgccagttgttgtgccacgcggttgggaatgtaattcagctccgccatcgccgcttccactttttcccgcgttttcgcagaaacgtggctggcctggttcaccacgcgggaaacggtctgataagagacaccggcatactctgcgacatcgtataacgttactggtttcacattcaccaccctgaattgactctcttccgggcgctatcatgccataccgcgaaaggttttgcgccattcgatggtgtcggatcgagatcgatctcgatcccgcgaaatttgacaattaatcatccggctcgtataatggaattgtgagcggataacaattcctactagagattaaagaggagaaagtactgccgggcctcttgcgggatatcgtccattccgacagcatcgccagtcactatggcgtgctgctagcgctatatgcgttgatgcaatttctatgcgcacccgttctcggagcactgtccgaccgc…
encoding: naseq.html

https://synbiohub.org/public/SEGA/SEGA061_seq/1
type: Sequence
persistent identity: SEGA061 seq
display id: SEGA061_seq
version: 1
owned by: carbay
owned by: morno
elements: gtgcatggtctgcaggcgctgccgagccgtattgagcagatgctgtctcaggacaaacgcattgaagcgctggcagaagatttctctgacaaacatcacgcgctgttcctgggccgtggcgatcagtacccaatcgcgctggaaggcgcattgaagttgaaagagatctcttacattcacgctgaagcctacgctgctggcgaactgaaacacggtccgctggcgctaattgatgccgatatgccggttattgttgttgcaccgaacaacgaattgctggaaaaactgaaatccaacattgaagaagttcgcgcgcgtggcggtcagttgtatgtcttcgccgatcaggatgcgggttttgtaagtagcgataacatgcacatcatcgagatgccgcatgtggaagaggtgattgcaccgatcttctacaccgttccgctgcagctgctggcttaccatgtcgcgctgatcaaaggcaccgacgttgaccagccgcgtaacctggcaaaatcggttacggttgagtaataaatggatctcggtaccaaattccagaaaagagacgctttcgagcgtcttttttcgttttggtccgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgccagggtggtttttcttttcaccagtgagacgggcaacagctgattgcccttcaccgcctggccctgagagagttgcagcaagcggtccacgctggtttgccccagcaggcgaaaatcctgtttgatggtggttaacggcgggatataacatgagctgtcttcggtatcgtcgtatcccactaccgagatgtccgcaccaacgcgcagcccggactcggtaatggcgcgcattgcgcccagcgccatctgatcgttggcaaccagcatcgcagtgggaacgatgccctcattcagcatttgcatggtttgttgaaaaccggacatggcactccagtcgccttcccgttccgctatcggctgaatttgattgcgagtgagatatttatgccagccagccagacgcagacgcgccgagacagaacttaatgggcccgctaacagcgcgatttgctggtgacccaatgcgaccagatgctccacgcccagtcgcgtaccgtcttcatgggagaaaataatactgttgatgggtgtctggtcagagacatcaagaaataacgccggaacattagtgcaggcagcttccacagcaatggcatcctggtcatccagcggatagttaatgatcagcccactgacgcgttgcgcgagaagattgtgcaccgccgctttacaggcttcgacgccgcttcgttctaccatcgacaccaccacgctggcacccagttgatcggcgcgagatttaatcgccgcgacaatttgcgacggcgcgtgcagggccagactggaggtggcaacgccaatcagcaacgactgtttgcccgccagttgttgtgccacgcggttgggaatgtaattcagctccgccatcgccgcttccactttttcccgcgttttcgcagaaacgtggctggcctggttcaccacgcgggaaacggtctgataagagacaccggcatactctgcgacatcgtataacgttactggtttcacattcaccaccctgaattgactctcttccgggcgctatcatgccataccgcgaaaggttttgcgccattcgatggtgtcggatcgagatcgatctcgatcccgcgaaattaatacgactcactataggggaattgtgagcggataacaattcctactagagattaaagaggagaaacattgtatgcagatattcgtgaaaacactgaccggcaaaaccattaccctggaggtggagcctggcagcgcccatcatcatcaccatcaccatcaccatcatgctggtagcagcgatacgatcgaaaacgtcaaagcca…
encoding: naseq.html

https://synbiohub.org/public/SEGA/SEGA050_seq/1
type: Sequence
persistent identity: SEGA050 seq
display id: SEGA050_seq
version: 1
owned by: carbay
owned by: morno
elements: gtgcatggtctgcaggcgctgccgagccgtattgagcagatgctgtctcaggacaaacgcattgaagcgctggcagaagatttctctgacaaacatcacgcgctgttcctgggccgtggcgatcagtacccaatcgcgctggaaggcgcattgaagttgaaagagatctcttacattcacgctgaagcctacgctgctggcgaactgaaacacggtccgctggcgctaattgatgccgatatgccggttattgttgttgcaccgaacaacgaattgctggaaaaactgaaatccaacattgaagaagttcgcgcgcgtggcggtcagttgtatgtcttcgccgatcaggatgcgggttttgtaagtagcgataacatgcacatcatcgagatgccgcatgtggaagaggtgattgcaccgatcttctacaccgttccgctgcagctgctggcttaccatgtcgcgctgatcaaaggcaccgacgttgaccagccgcgtaacctggcaaaatcggttacggttgagtaataaatggatctcggtaccaaattccagaaaagagacgctttcgagcgtcttttttcgttttggtccgcgttgcgcttaatctttctgcgaattgagatgacgccactggctgggcgtcatcccggtttcccgggtaaacaccaccgaaaaatagttactatcttcaaagccacattcggtcgaaatatcactgattaacaggcggctatgctggagaagatattgcgcatgacacactctgacctgtcgcagatattgattgatggtcattccagtctgctggcgaaattgctgacgcaaaacgcgctcactgcacgatgcctcatcacaaaatttatccagcgcaaagggacttttcaggctagccgccagccgggtaatcagcttatccagcaacgtttcgctggatgttggcggcaacgaatcactggtgtaacgatggcgattcagcaacatcaccaactgcccgaacagcaactcagccatttcgttagcaaacggcacatgctgactactttcatgctcaagctgaccaataacctgccgcgcctgcgccatccccatgctacctaagcgccagtgtggttgccctgcgctggcgttaaatcccggaatcgccccctgccagtcaagattcagcttcagacgctccgggcaataaataatattctgcaaaaccagatcgttaacggaagcgtaggagtgtttatcatcagcatgaatgtaaaagagatcgccacgggtaatgcgataagggcgatcgttgagtacatgcaggccattaccgcgccagacaatcaccagctcacaaaaatcatgtgtatgttcagcaaagacatcttgcggataacggtcagccacagcgactgcctgctggtcgctggcaaaaaaatcatctttgagaagttttaactgatgcgccaccgtggctacctcggccagagaacgaagttgattattcgcaatatggcgtacaaatacgttgagaagattcgcgttattgcagaaagccatcccgtccctggcgaatatcacgcggtgaccagttaaactctcggcgaaaaagcgtcgaaaagtggttactgtcgctgaatccacagcgataggcgatgtcagtaacgctggcctcgctgtggcgtagcagatgtcgggctttcatcagtcgcaggcggttcaggtatcgctgaggcgtcagtcccgtttgctgcttaagctgccgatgtagcgtacgcagtgaaagagaaaattgatccgccacggcatcccaattcacctcatcggcaaaatggtcctccagccaggccagaagcaagttgagacgtgatgcgctgttttccaggttctcctgcaaactgcttttacgcagcaagagcagtaattgcataaacaagatctcgcgactggcggtcgagggtaaatcattttccccttcctgctgttccatctgtgcaaccagctgtcgcacctgctgc…
encoding: naseq.html

https://synbiohub.org/public/SEGA/SEGA048_seq/1
type: Sequence
persistent identity: SEGA048 seq
display id: SEGA048_seq
version: 1
owned by: carbay
owned by: morno
elements: gtgcatggtctgcaggcgctgccgagccgtattgagcagatgctgtctcaggacaaacgcattgaagcgctggcagaagatttctctgacaaacatcacgcgctgttcctgggccgtggcgatcagtacccaatcgcgctggaaggcgcattgaagttgaaagagatctcttacattcacgctgaagcctacgctgctggcgaactgaaacacggtccgctggcgctaattgatgccgatatgccggttattgttgttgcaccgaacaacgaattgctggaaaaactgaaatccaacattgaagaagttcgcgcgcgtggcggtcagttgtatgtcttcgccgatcaggatgcgggttttgtaagtagcgataacatgcacatcatcgagatgccgcatgtggaagaggtgattgcaccgatcttctacaccgttccgctgcagctgctggcttaccatgtcgcgctgatcaaaggcaccgacgttgaccagccgcgtaacctggcaaaatcggttacggttgagtaataaatggatctcggtaccaaattccagaaaagagacgctttcgagcgtcttttttcgttttggtccgcgttgcgcttaatctttctgcgaattgagatgacgccactggctgggcgtcatcccggtttcccgggtaaacaccaccgaaaaatagttactatcttcaaagccacattcggtcgaaatatcactgattaacaggcggctatgctggagaagatattgcgcatgacacactctgacctgtcgcagatattgattgatggtcattccagtctgctggcgaaattgctgacgcaaaacgcgctcactgcacgatgcctcatcacaaaatttatccagcgcaaagggacttttcaggctagccgccagccgggtaatcagcttatccagcaacgtttcgctggatgttggcggcaacgaatcactggtgtaacgatggcgattcagcaacatcaccaactgcccgaacagcaactcagccatttcgttagcaaacggcacatgctgactactttcatgctcaagctgaccaataacctgccgcgcctgcgccatccccatgctacctaagcgccagtgtggttgccctgcgctggcgttaaatcccggaatcgccccctgccagtcaagattcagcttcagacgctccgggcaataaataatattctgcaaaaccagatcgttaacggaagcgtaggagtgtttatcatcagcatgaatgtaaaagagatcgccacgggtaatgcgataagggcgatcgttgagtacatgcaggccattaccgcgccagacaatcaccagctcacaaaaatcatgtgtatgttcagcaaagacatcttgcggataacggtcagccacagcgactgcctgctggtcgctggcaaaaaaatcatctttgagaagttttaactgatgcgccaccgtggctacctcggccagagaacgaagttgattattcgcaatatggcgtacaaatacgttgagaagattcgcgttattgcagaaagccatcccgtccctggcgaatatcacgcggtgaccagttaaactctcggcgaaaaagcgtcgaaaagtggttactgtcgctgaatccacagcgataggcgatgtcagtaacgctggcctcgctgtggcgtagcagatgtcgggctttcatcagtcgcaggcggttcaggtatcgctgaggcgtcagtcccgtttgctgcttaagctgccgatgtagcgtacgcagtgaaagagaaaattgatccgccacggcatcccaattcacctcatcggcaaaatggtcctccagccaggccagaagcaagttgagacgtgatgcgctgttttccaggttctcctgcaaactgcttttacgcagcaagagcagtaattgcataaacaagatctcgcgactggcggtcgagggtaaatcattttccccttcctgctgttccatctgtgcaaccagctgtcgcacctgctgc…
encoding: naseq.html

https://synbiohub.org/public/SEGA/SEGA035_seq/1
type: Sequence
persistent identity: SEGA035 seq
display id: SEGA035_seq
version: 1
owned by: carbay
owned by: morno
elements: gtgcatggtctgcaggcgctgccgagccgtattgagcagatgctgtctcaggacaaacgcattgaagcgctggcagaagatttctctgacaaacatcacgcgctgttcctgggccgtggcgatcagtacccaatcgcgctggaaggcgcattgaagttgaaagagatctcttacattcacgctgaagcctacgctgctggcgaactgaaacacggtccgctggcgctaattgatgccgatatgccggttattgttgttgcaccgaacaacgaattgctggaaaaactgaaatccaacattgaagaagttcgcgcgcgtggcggtcagttgtatgtcttcgccgatcaggatgcgggttttgtaagtagcgataacatgcacatcatcgagatgccgcatgtggaagaggtgattgcaccgatcttctacaccgttccgctgcagctgctggcttaccatgtcgcgctgatcaaaggcaccgacgttgaccagccgcgtaacctggcaaaatcggttacggttgagtaataaatggatctcggtaccaaattccagaaaagagacgctttcgagcgtcttttttcgttttggtccgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgccagggtggtttttcttttcaccagtgagacgggcaacagctgattgcccttcaccgcctggccctgagagagttgcagcaagcggtccacgctggtttgccccagcaggcgaaaatcctgtttgatggtggttaacggcgggatataacatgagctgtcttcggtatcgtcgtatcccactaccgagatgtccgcaccaacgcgcagcccggactcggtaatggcgcgcattgcgcccagcgccatctgatcgttggcaaccagcatcgcagtgggaacgatgccctcattcagcatttgcatggtttgttgaaaaccggacatggcactccagtcgccttcccgttccgctatcggctgaatttgattgcgagtgagatatttatgccagccagccagacgcagacgcgccgagacagaacttaatgggcccgctaacagcgcgatttgctggtgacccaatgcgaccagatgctccacgcccagtcgcgtaccgtcttcatgggagaaaataatactgttgatgggtgtctggtcagagacatcaagaaataacgccggaacattagtgcaggcagcttccacagcaatggcatcctggtcatccagcggatagttaatgatcagcccactgacgcgttgcgcgagaagattgtgcaccgccgctttacaggcttcgacgccgcttcgttctaccatcgacaccaccacgctggcacccagttgatcggcgcgagatttaatcgccgcgacaatttgcgacggcgcgtgcagggccagactggaggtggcaacgccaatcagcaacgactgtttgcccgccagttgttgtgccacgcggttgggaatgtaattcagctccgccatcgccgcttccactttttcccgcgttttcgcagaaacgtggctggcctggttcaccacgcgggaaacggtctgataagagacaccggcatactctgcgacatcgtataacgttactggtttcacattcaccaccctgaattgactctcttccgggcgctatcatgccataccgcgaaaggttttgcgccattcgatggtgtcggatcgagatcgatctcgatcccgcgaaatttgacaattaatcatccggctcgtataatggaattgtgagcggataacaattcctactagagattaaagaggagaaaccctcgatgaagaaaagaggggcgtttttagggctgttgttggtttctgcctgcgcatcagttttcgctgcgaataacgaaacgagcaagagcgtcaccttcccgaagtgcgaggatctggacgcagcg…
encoding: naseq.html

https://synbiohub.org/public/SEGA/SEGA021_seq/1
type: Sequence
persistent identity: SEGA021 seq
display id: SEGA021_seq
version: 1
owned by: carbay
owned by: morno
elements: gtgcatggtctgcaggcgctgccgagccgtattgagcagatgctgtctcaggacaaacgcattgaagcgctggcagaagatttctctgacaaacatcacgcgctgttcctgggccgtggcgatcagtacccaatcgcgctggaaggcgcattgaagttgaaagagatctcttacattcacgctgaagcctacgctgctggcgaactgaaacacggtccgctggcgctaattgatgccgatatgccggttattgttgttgcaccgaacaacgaattgctggaaaaactgaaatccaacattgaagaagttcgcgcgcgtggcggtcagttgtatgtcttcgccgatcaggatgcgggttttgtaagtagcgataacatgcacatcatcgagatgccgcatgtggaagaggtgattgcaccgatcttctacaccgttccgctgcagctgctggcttaccatgtcgcgctgatcaaaggcaccgacgttgaccagccgcgtaacctggcaaaatcggttacggttgagtaataaatggatctcggtaccaaattccagaaaagagacgctttcgagcgtcttttttcgttttggtccgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgccagggtggtttttcttttcaccagtgagacgggcaacagctgattgcccttcaccgcctggccctgagagagttgcagcaagcggtccacgctggtttgccccagcaggcgaaaatcctgtttgatggtggttaacggcgggatataacatgagctgtcttcggtatcgtcgtatcccactaccgagatgtccgcaccaacgcgcagcccggactcggtaatggcgcgcattgcgcccagcgccatctgatcgttggcaaccagcatcgcagtgggaacgatgccctcattcagcatttgcatggtttgttgaaaaccggacatggcactccagtcgccttcccgttccgctatcggctgaatttgattgcgagtgagatatttatgccagccagccagacgcagacgcgccgagacagaacttaatgggcccgctaacagcgcgatttgctggtgacccaatgcgaccagatgctccacgcccagtcgcgtaccgtcttcatgggagaaaataatactgttgatgggtgtctggtcagagacatcaagaaataacgccggaacattagtgcaggcagcttccacagcaatggcatcctggtcatccagcggatagttaatgatcagcccactgacgcgttgcgcgagaagattgtgcaccgccgctttacaggcttcgacgccgcttcgttctaccatcgacaccaccacgctggcacccagttgatcggcgcgagatttaatcgccgcgacaatttgcgacggcgcgtgcagggccagactggaggtggcaacgccaatcagcaacgactgtttgcccgccagttgttgtgccacgcggttgggaatgtaattcagctccgccatcgccgcttccactttttcccgcgttttcgcagaaacgtggctggcctggttcaccacgcgggaaacggtctgataagagacaccggcatactctgcgacatcgtataacgttactggtttcacattcaccaccctgaattgactctcttccgggcgctatcatgccataccgcgaaaggttttgcgccattcgatggtgtcggatcgagatcgatctcgatcccgcgaaatttgacaattaatcatccggctcgtataatggaattgtgagcggataacaattcctactagagattaaagaggagaaagagctaatgaaggctattttcgtactgaaacatcttaatcatgctaaggaggttttctaatgagcaaaggagaagaacttttcactggagttgtgccaattcttgttgaattagatggtgatgttaatg…
encoding: naseq.html

https://synbiohub.org/public/SEGA/SEGA057_seq/1
type: Sequence
persistent identity: SEGA057 seq
display id: SEGA057_seq
version: 1
owned by: carbay
owned by: morno
elements: gtgcatggtctgcaggcgctgccgagccgtattgagcagatgctgtctcaggacaaacgcattgaagcgctggcagaagatttctctgacaaacatcacgcgctgttcctgggccgtggcgatcagtacccaatcgcgctggaaggcgcattgaagttgaaagagatctcttacattcacgctgaagcctacgctgctggcgaactgaaacacggtccgctggcgctaattgatgccgatatgccggttattgttgttgcaccgaacaacgaattgctggaaaaactgaaatccaacattgaagaagttcgcgcgcgtggcggtcagttgtatgtcttcgccgatcaggatgcgggttttgtaagtagcgataacatgcacatcatcgagatgccgcatgtggaagaggtgattgcaccgatcttctacaccgttccgctgcagctgctggcttaccatgtcgcgctgatcaaaggcaccgacgttgaccagccgcgtaacctggcaaaatcggttacggttgagtaataaatggatctcggtaccaaattccagaaaagagacgctttcgagcgtcttttttcgttttggtccgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgccagggtggtttttcttttcaccagtgagacgggcaacagctgattgcccttcaccgcctggccctgagagagttgcagcaagcggtccacgctggtttgccccagcaggcgaaaatcctgtttgatggtggttaacggcgggatataacatgagctgtcttcggtatcgtcgtatcccactaccgagatgtccgcaccaacgcgcagcccggactcggtaatggcgcgcattgcgcccagcgccatctgatcgttggcaaccagcatcgcagtgggaacgatgccctcattcagcatttgcatggtttgttgaaaaccggacatggcactccagtcgccttcccgttccgctatcggctgaatttgattgcgagtgagatatttatgccagccagccagacgcagacgcgccgagacagaacttaatgggcccgctaacagcgcgatttgctggtgacccaatgcgaccagatgctccacgcccagtcgcgtaccgtcttcatgggagaaaataatactgttgatgggtgtctggtcagagacatcaagaaataacgccggaacattagtgcaggcagcttccacagcaatggcatcctggtcatccagcggatagttaatgatcagcccactgacgcgttgcgcgagaagattgtgcaccgccgctttacaggcttcgacgccgcttcgttctaccatcgacaccaccacgctggcacccagttgatcggcgcgagatttaatcgccgcgacaatttgcgacggcgcgtgcagggccagactggaggtggcaacgccaatcagcaacgactgtttgcccgccagttgttgtgccacgcggttgggaatgtaattcagctccgccatcgccgcttccactttttcccgcgttttcgcagaaacgtggctggcctggttcaccacgcgggaaacggtctgataagagacaccggcatactctgcgacatcgtataacgttactggtttcacattcaccaccctgaattgactctcttccgggcgctatcatgccataccgcgaaaggttttgcgccattcgatggtgtcggatcgagatcgatctcgatcccgcgaaattaatacgactcactataggggaattgtgagcggataacaattcctactagagattaaagaggagaaagagctaatgaaggctattttcgtactgaaacatcttaatcatgctaaggaggttttctaatgagcaaaggagaagaacttttcactggagttgtgccaattcttgttgaattagatggtgatgttaatgggcacaaatt…
encoding: naseq.html

https://synbiohub.org/public/SEGA/SEGA056_seq/1
type: Sequence
persistent identity: SEGA056 seq
display id: SEGA056_seq
version: 1
owned by: carbay
owned by: morno
elements: gtgcatggtctgcaggcgctgccgagccgtattgagcagatgctgtctcaggacaaacgcattgaagcgctggcagaagatttctctgacaaacatcacgcgctgttcctgggccgtggcgatcagtacccaatcgcgctggaaggcgcattgaagttgaaagagatctcttacattcacgctgaagcctacgctgctggcgaactgaaacacggtccgctggcgctaattgatgccgatatgccggttattgttgttgcaccgaacaacgaattgctggaaaaactgaaatccaacattgaagaagttcgcgcgcgtggcggtcagttgtatgtcttcgccgatcaggatgcgggttttgtaagtagcgataacatgcacatcatcgagatgccgcatgtggaagaggtgattgcaccgatcttctacaccgttccgctgcagctgctggcttaccatgtcgcgctgatcaaaggcaccgacgttgaccagccgcgtaacctggcaaaatcggttacggttgagtaataaatggatctcggtaccaaattccagaaaagagacgctttcgagcgtcttttttcgttttggtccgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgccagggtggtttttcttttcaccagtgagacgggcaacagctgattgcccttcaccgcctggccctgagagagttgcagcaagcggtccacgctggtttgccccagcaggcgaaaatcctgtttgatggtggttaacggcgggatataacatgagctgtcttcggtatcgtcgtatcccactaccgagatgtccgcaccaacgcgcagcccggactcggtaatggcgcgcattgcgcccagcgccatctgatcgttggcaaccagcatcgcagtgggaacgatgccctcattcagcatttgcatggtttgttgaaaaccggacatggcactccagtcgccttcccgttccgctatcggctgaatttgattgcgagtgagatatttatgccagccagccagacgcagacgcgccgagacagaacttaatgggcccgctaacagcgcgatttgctggtgacccaatgcgaccagatgctccacgcccagtcgcgtaccgtcttcatgggagaaaataatactgttgatgggtgtctggtcagagacatcaagaaataacgccggaacattagtgcaggcagcttccacagcaatggcatcctggtcatccagcggatagttaatgatcagcccactgacgcgttgcgcgagaagattgtgcaccgccgctttacaggcttcgacgccgcttcgttctaccatcgacaccaccacgctggcacccagttgatcggcgcgagatttaatcgccgcgacaatttgcgacggcgcgtgcagggccagactggaggtggcaacgccaatcagcaacgactgtttgcccgccagttgttgtgccacgcggttgggaatgtaattcagctccgccatcgccgcttccactttttcccgcgttttcgcagaaacgtggctggcctggttcaccacgcgggaaacggtctgataagagacaccggcatactctgcgacatcgtataacgttactggtttcacattcaccaccctgaattgactctcttccgggcgctatcatgccataccgcgaaaggttttgcgccattcgatggtgtcggatcgagatcgatctcgatcccgcgaaattaatacgactcactataggggaattgtgagcggataacaattcctactagagattaaagaggagaaaggggggatgaaggctattttcgtactgaaacatcttaatcatgctaaggaggttttctaatgagcaaaggagaagaacttttcactggagttgtgccaattcttgttgaattagatggtgatgttaatgggcacaaatt…
encoding: naseq.html

https://synbiohub.org/public/SEGA/SEGA072_seq/1
type: Sequence
persistent identity: SEGA072 seq
display id: SEGA072_seq
version: 1
owned by: carbay
owned by: morno
elements: gtgcatggtctgcaggcgctgccgagccgtattgagcagatgctgtctcaggacaaacgcattgaagcgctggcagaagatttctctgacaaacatcacgcgctgttcctgggccgtggcgatcagtacccaatcgcgctggaaggcgcattgaagttgaaagagatctcttacattcacgctgaagcctacgctgctggcgaactgaaacacggtccgctggcgctaattgatgccgatatgccggttattgttgttgcaccgaacaacgaattgctggaaaaactgaaatccaacattgaagaagttcgcgcgcgtggcggtcagttgtatgtcttcgccgatcaggatgcgggttttgtaagtagcgataacatgcacatcatcgagatgccgcatgtggaagaggtgattgcaccgatcttctacaccgttccgctgcagctgctggcttaccatgtcgcgctgatcaaaggcaccgacgttgaccagccgcgtaacctggcaaaatcggttacggttgagtaataaatggatctcggtaccaaattccagaaaagagacgctttcgagcgtcttttttcgttttggtccgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgccagggtggtttttcttttcaccagtgagacgggcaacagctgattgcccttcaccgcctggccctgagagagttgcagcaagcggtccacgctggtttgccccagcaggcgaaaatcctgtttgatggtggttaacggcgggatataacatgagctgtcttcggtatcgtcgtatcccactaccgagatgtccgcaccaacgcgcagcccggactcggtaatggcgcgcattgcgcccagcgccatctgatcgttggcaaccagcatcgcagtgggaacgatgccctcattcagcatttgcatggtttgttgaaaaccggacatggcactccagtcgccttcccgttccgctatcggctgaatttgattgcgagtgagatatttatgccagccagccagacgcagacgcgccgagacagaacttaatgggcccgctaacagcgcgatttgctggtgacccaatgcgaccagatgctccacgcccagtcgcgtaccgtcttcatgggagaaaataatactgttgatgggtgtctggtcagagacatcaagaaataacgccggaacattagtgcaggcagcttccacagcaatggcatcctggtcatccagcggatagttaatgatcagcccactgacgcgttgcgcgagaagattgtgcaccgccgctttacaggcttcgacgccgcttcgttctaccatcgacaccaccacgctggcacccagttgatcggcgcgagatttaatcgccgcgacaatttgcgacggcgcgtgcagggccagactggaggtggcaacgccaatcagcaacgactgtttgcccgccagttgttgtgccacgcggttgggaatgtaattcagctccgccatcgccgcttccactttttcccgcgttttcgcagaaacgtggctggcctggttcaccacgcgggaaacggtctgataagagacaccggcatactctgcgacatcgtataacgttactggtttcacattcaccaccctgaattgactctcttccgggcgctatcatgccataccgcgaaaggttttgcgccattcgatggtgtcggatcgagatcgatctcgatcccgcgaaattaatacgactcactataggggaattgtgagcggataacaattcctactagagattaaagaggagaaagactggatgaaaaagagaggggcgtttttagggctgttgttggtttctgcctgcgcatcagttttcgctgcgaataacgaaacgagcaagagcgtcaccttcccgaagtgcgaggatctggacgcagcgggcattgctg…
encoding: naseq.html

https://synbiohub.org/public/SEGA/SEGA054_seq/1
type: Sequence
persistent identity: SEGA054 seq
display id: SEGA054_seq
version: 1
owned by: carbay
owned by: morno
elements: gtgcatggtctgcaggcgctgccgagccgtattgagcagatgctgtctcaggacaaacgcattgaagcgctggcagaagatttctctgacaaacatcacgcgctgttcctgggccgtggcgatcagtacccaatcgcgctggaaggcgcattgaagttgaaagagatctcttacattcacgctgaagcctacgctgctggcgaactgaaacacggtccgctggcgctaattgatgccgatatgccggttattgttgttgcaccgaacaacgaattgctggaaaaactgaaatccaacattgaagaagttcgcgcgcgtggcggtcagttgtatgtcttcgccgatcaggatgcgggttttgtaagtagcgataacatgcacatcatcgagatgccgcatgtggaagaggtgattgcaccgatcttctacaccgttccgctgcagctgctggcttaccatgtcgcgctgatcaaaggcaccgacgttgaccagccgcgtaacctggcaaaatcggttacggttgagtaataaatggatctcggtaccaaattccagaaaagagacgctttcgagcgtcttttttcgttttggtccgcgttgcgcttaatctttctgcgaattgagatgacgccactggctgggcgtcatcccggtttcccgggtaaacaccaccgaaaaatagttactatcttcaaagccacattcggtcgaaatatcactgattaacaggcggctatgctggagaagatattgcgcatgacacactctgacctgtcgcagatattgattgatggtcattccagtctgctggcgaaattgctgacgcaaaacgcgctcactgcacgatgcctcatcacaaaatttatccagcgcaaagggacttttcaggctagccgccagccgggtaatcagcttatccagcaacgtttcgctggatgttggcggcaacgaatcactggtgtaacgatggcgattcagcaacatcaccaactgcccgaacagcaactcagccatttcgttagcaaacggcacatgctgactactttcatgctcaagctgaccaataacctgccgcgcctgcgccatccccatgctacctaagcgccagtgtggttgccctgcgctggcgttaaatcccggaatcgccccctgccagtcaagattcagcttcagacgctccgggcaataaataatattctgcaaaaccagatcgttaacggaagcgtaggagtgtttatcatcagcatgaatgtaaaagagatcgccacgggtaatgcgataagggcgatcgttgagtacatgcaggccattaccgcgccagacaatcaccagctcacaaaaatcatgtgtatgttcagcaaagacatcttgcggataacggtcagccacagcgactgcctgctggtcgctggcaaaaaaatcatctttgagaagttttaactgatgcgccaccgtggctacctcggccagagaacgaagttgattattcgcaatatggcgtacaaatacgttgagaagattcgcgttattgcagaaagccatcccgtccctggcgaatatcacgcggtgaccagttaaactctcggcgaaaaagcgtcgaaaagtggttactgtcgctgaatccacagcgataggcgatgtcagtaacgctggcctcgctgtggcgtagcagatgtcgggctttcatcagtcgcaggcggttcaggtatcgctgaggcgtcagtcccgtttgctgcttaagctgccgatgtagcgtacgcagtgaaagagaaaattgatccgccacggcatcccaattcacctcatcggcaaaatggtcctccagccaggccagaagcaagttgagacgtgatgcgctgttttccaggttctcctgcaaactgcttttacgcagcaagagcagtaattgcataaacaagatctcgcgactggcggtcgagggtaaatcattttccccttcctgctgttccatctgtgcaaccagctgtcgcacctgctgc…
encoding: naseq.html

https://synbiohub.org/public/SEGA/SEGA023_seq/1
type: Sequence
persistent identity: SEGA023 seq
display id: SEGA023_seq
version: 1
owned by: carbay
owned by: morno
elements: gtgcatggtctgcaggcgctgccgagccgtattgagcagatgctgtctcaggacaaacgcattgaagcgctggcagaagatttctctgacaaacatcacgcgctgttcctgggccgtggcgatcagtacccaatcgcgctggaaggcgcattgaagttgaaagagatctcttacattcacgctgaagcctacgctgctggcgaactgaaacacggtccgctggcgctaattgatgccgatatgccggttattgttgttgcaccgaacaacgaattgctggaaaaactgaaatccaacattgaagaagttcgcgcgcgtggcggtcagttgtatgtcttcgccgatcaggatgcgggttttgtaagtagcgataacatgcacatcatcgagatgccgcatgtggaagaggtgattgcaccgatcttctacaccgttccgctgcagctgctggcttaccatgtcgcgctgatcaaaggcaccgacgttgaccagccgcgtaacctggcaaaatcggttacggttgagtaataaatggatctcggtaccaaattccagaaaagagacgctttcgagcgtcttttttcgttttggtccgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgccagggtggtttttcttttcaccagtgagacgggcaacagctgattgcccttcaccgcctggccctgagagagttgcagcaagcggtccacgctggtttgccccagcaggcgaaaatcctgtttgatggtggttaacggcgggatataacatgagctgtcttcggtatcgtcgtatcccactaccgagatgtccgcaccaacgcgcagcccggactcggtaatggcgcgcattgcgcccagcgccatctgatcgttggcaaccagcatcgcagtgggaacgatgccctcattcagcatttgcatggtttgttgaaaaccggacatggcactccagtcgccttcccgttccgctatcggctgaatttgattgcgagtgagatatttatgccagccagccagacgcagacgcgccgagacagaacttaatgggcccgctaacagcgcgatttgctggtgacccaatgcgaccagatgctccacgcccagtcgcgtaccgtcttcatgggagaaaataatactgttgatgggtgtctggtcagagacatcaagaaataacgccggaacattagtgcaggcagcttccacagcaatggcatcctggtcatccagcggatagttaatgatcagcccactgacgcgttgcgcgagaagattgtgcaccgccgctttacaggcttcgacgccgcttcgttctaccatcgacaccaccacgctggcacccagttgatcggcgcgagatttaatcgccgcgacaatttgcgacggcgcgtgcagggccagactggaggtggcaacgccaatcagcaacgactgtttgcccgccagttgttgtgccacgcggttgggaatgtaattcagctccgccatcgccgcttccactttttcccgcgttttcgcagaaacgtggctggcctggttcaccacgcgggaaacggtctgataagagacaccggcatactctgcgacatcgtataacgttactggtttcacattcaccaccctgaattgactctcttccgggcgctatcatgccataccgcgaaaggttttgcgccattcgatggtgtcggatcgagatcgatctcgatcccgcgaaatttgacaattaatcatccggctcgtataatggaattgtgagcggataacaattcctactagagattaaagaggagaaaggggggatgcaaattttcgtgaaaacactgaccggcaaaaccattaccctggaggtggagcctggcagcgcccatcatcatcaccatcaccatcaccatcatgctggtagcagcgatacgatcgaaaac…
encoding: naseq.html

https://synbiohub.org/public/SEGA/SEGA064_seq/1
type: Sequence
persistent identity: SEGA064 seq
display id: SEGA064_seq
version: 1
owned by: carbay
owned by: morno
elements: gtgcatggtctgcaggcgctgccgagccgtattgagcagatgctgtctcaggacaaacgcattgaagcgctggcagaagatttctctgacaaacatcacgcgctgttcctgggccgtggcgatcagtacccaatcgcgctggaaggcgcattgaagttgaaagagatctcttacattcacgctgaagcctacgctgctggcgaactgaaacacggtccgctggcgctaattgatgccgatatgccggttattgttgttgcaccgaacaacgaattgctggaaaaactgaaatccaacattgaagaagttcgcgcgcgtggcggtcagttgtatgtcttcgccgatcaggatgcgggttttgtaagtagcgataacatgcacatcatcgagatgccgcatgtggaagaggtgattgcaccgatcttctacaccgttccgctgcagctgctggcttaccatgtcgcgctgatcaaaggcaccgacgttgaccagccgcgtaacctggcaaaatcggttacggttgagtaataaatggatctcggtaccaaattccagaaaagagacgctttcgagcgtcttttttcgttttggtccgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgccagggtggtttttcttttcaccagtgagacgggcaacagctgattgcccttcaccgcctggccctgagagagttgcagcaagcggtccacgctggtttgccccagcaggcgaaaatcctgtttgatggtggttaacggcgggatataacatgagctgtcttcggtatcgtcgtatcccactaccgagatgtccgcaccaacgcgcagcccggactcggtaatggcgcgcattgcgcccagcgccatctgatcgttggcaaccagcatcgcagtgggaacgatgccctcattcagcatttgcatggtttgttgaaaaccggacatggcactccagtcgccttcccgttccgctatcggctgaatttgattgcgagtgagatatttatgccagccagccagacgcagacgcgccgagacagaacttaatgggcccgctaacagcgcgatttgctggtgacccaatgcgaccagatgctccacgcccagtcgcgtaccgtcttcatgggagaaaataatactgttgatgggtgtctggtcagagacatcaagaaataacgccggaacattagtgcaggcagcttccacagcaatggcatcctggtcatccagcggatagttaatgatcagcccactgacgcgttgcgcgagaagattgtgcaccgccgctttacaggcttcgacgccgcttcgttctaccatcgacaccaccacgctggcacccagttgatcggcgcgagatttaatcgccgcgacaatttgcgacggcgcgtgcagggccagactggaggtggcaacgccaatcagcaacgactgtttgcccgccagttgttgtgccacgcggttgggaatgtaattcagctccgccatcgccgcttccactttttcccgcgttttcgcagaaacgtggctggcctggttcaccacgcgggaaacggtctgataagagacaccggcatactctgcgacatcgtataacgttactggtttcacattcaccaccctgaattgactctcttccgggcgctatcatgccataccgcgaaaggttttgcgccattcgatggtgtcggatcgagatcgatctcgatcccgcgaaattaatacgactcactataggggaattgtgagcggataacaattcctactagagattaaagaggagaaaatatcgatggagaacctgtacttccagagcaaaggagaagaacttttcactggagttgtgccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgctacat…
encoding: naseq.html

https://synbiohub.org/public/SEGA/SEGA052_seq/1
type: Sequence
persistent identity: SEGA052 seq
display id: SEGA052_seq
version: 1
owned by: carbay
owned by: morno
elements: gtgcatggtctgcaggcgctgccgagccgtattgagcagatgctgtctcaggacaaacgcattgaagcgctggcagaagatttctctgacaaacatcacgcgctgttcctgggccgtggcgatcagtacccaatcgcgctggaaggcgcattgaagttgaaagagatctcttacattcacgctgaagcctacgctgctggcgaactgaaacacggtccgctggcgctaattgatgccgatatgccggttattgttgttgcaccgaacaacgaattgctggaaaaactgaaatccaacattgaagaagttcgcgcgcgtggcggtcagttgtatgtcttcgccgatcaggatgcgggttttgtaagtagcgataacatgcacatcatcgagatgccgcatgtggaagaggtgattgcaccgatcttctacaccgttccgctgcagctgctggcttaccatgtcgcgctgatcaaaggcaccgacgttgaccagccgcgtaacctggcaaaatcggttacggttgagtaataaatggatctcggtaccaaattccagaaaagagacgctttcgagcgtcttttttcgttttggtccgcgttgcgcttaatctttctgcgaattgagatgacgccactggctgggcgtcatcccggtttcccgggtaaacaccaccgaaaaatagttactatcttcaaagccacattcggtcgaaatatcactgattaacaggcggctatgctggagaagatattgcgcatgacacactctgacctgtcgcagatattgattgatggtcattccagtctgctggcgaaattgctgacgcaaaacgcgctcactgcacgatgcctcatcacaaaatttatccagcgcaaagggacttttcaggctagccgccagccgggtaatcagcttatccagcaacgtttcgctggatgttggcggcaacgaatcactggtgtaacgatggcgattcagcaacatcaccaactgcccgaacagcaactcagccatttcgttagcaaacggcacatgctgactactttcatgctcaagctgaccaataacctgccgcgcctgcgccatccccatgctacctaagcgccagtgtggttgccctgcgctggcgttaaatcccggaatcgccccctgccagtcaagattcagcttcagacgctccgggcaataaataatattctgcaaaaccagatcgttaacggaagcgtaggagtgtttatcatcagcatgaatgtaaaagagatcgccacgggtaatgcgataagggcgatcgttgagtacatgcaggccattaccgcgccagacaatcaccagctcacaaaaatcatgtgtatgttcagcaaagacatcttgcggataacggtcagccacagcgactgcctgctggtcgctggcaaaaaaatcatctttgagaagttttaactgatgcgccaccgtggctacctcggccagagaacgaagttgattattcgcaatatggcgtacaaatacgttgagaagattcgcgttattgcagaaagccatcccgtccctggcgaatatcacgcggtgaccagttaaactctcggcgaaaaagcgtcgaaaagtggttactgtcgctgaatccacagcgataggcgatgtcagtaacgctggcctcgctgtggcgtagcagatgtcgggctttcatcagtcgcaggcggttcaggtatcgctgaggcgtcagtcccgtttgctgcttaagctgccgatgtagcgtacgcagtgaaagagaaaattgatccgccacggcatcccaattcacctcatcggcaaaatggtcctccagccaggccagaagcaagttgagacgtgatgcgctgttttccaggttctcctgcaaactgcttttacgcagcaagagcagtaattgcataaacaagatctcgcgactggcggtcgagggtaaatcattttccccttcctgctgttccatctgtgcaaccagctgtcgcacctgctgc…
encoding: naseq.html

https://synbiohub.org/public/SEGA/SEGA075_seq/1
type: Sequence
persistent identity: SEGA075 seq
display id: SEGA075_seq
version: 1
owned by: carbay
owned by: morno
elements: gtgcatggtctgcaggcgctgccgagccgtattgagcagatgctgtctcaggacaaacgcattgaagcgctggcagaagatttctctgacaaacatcacgcgctgttcctgggccgtggcgatcagtacccaatcgcgctggaaggcgcattgaagttgaaagagatctcttacattcacgctgaagcctacgctgctggcgaactgaaacacggtccgctggcgctaattgatgccgatatgccggttattgttgttgcaccgaacaacgaattgctggaaaaactgaaatccaacattgaagaagttcgcgcgcgtggcggtcagttgtatgtcttcgccgatcaggatgcgggttttgtaagtagcgataacatgcacatcatcgagatgccgcatgtggaagaggtgattgcaccgatcttctacaccgttccgctgcagctgctggcttaccatgtcgcgctgatcaaaggcaccgacgttgaccagccgcgtaacctggcaaaatcggttacggttgagtaataaatggatctcggtaccaaattccagaaaagagacgctttcgagcgtcttttttcgttttggtccccaattaggatccttatcaatccgtaaacaggtcaaacatcagttgccgcaaccaaatattggctaggtccttgtggtacttcgcatgccagaacatgttgatggctatttcaggcaagacgactgggtgcggcaaggcgcttaggccgaagggctctacgcagcagtcggctaaacatatcggcacagtggcgagcagatcggtgcgctggaggatgtggccaacggcggcgaagtgcggcacttccagacggatgtcgcgccggatgccgacccgtgtcatgtacgtgtccacctcgccgtggccggtgccagcggcgatgacacgcacgtggccgtaggaacagaagcgctccagagtcaggggttcgcgggtgactggatggtccttgcgacataggcacacgtagtgattctggagcagccggcgctgaaagaagccagtttgcagattgggaagcaggcccacggccaagtccacggttccgttctgcaaggcctgcatcaggctcatcgaactgtcgcgcaccgtactgatcacgcaattgggggcctggtgagccagcgcatccatcagccgcggcatgaagtagatctcgccaatgtcggtcatggccagggtgaaggtacgctcgctggtcagcggatcgaagctttcatggtgctgtagggcgttgcgcagtgcgtgcatggccgaagtgacgtgctcggccagatgcgcggcatagggtgtgggttccattccctgatgtgtgcgcacgaagagtgggtcctgtagcgaggtgcgcaggcgtttcagcgcattgctcacggcaggctgggtcaggcccaggttctccgcagtgacagagacgcgtctgtcgaccagcaactggttgaacaccaccagcaggtttaaatccaggtcacgcagttccatattcaacccccatgaattgactctcttccgggcgctatcatgccataccgcgaaaggttttgcaccattcgatggcgcgccgccattggagcttaacgatcgttggctgggggcctcgcttgggttattgctggtgcccggccgggcgcaatattcatgttgatgatttattatatatcgagtggtgtatttatttatattgtttgctccgttaccgttattaacagctgtcaccggatgtgctttccggtctgatgagtccgtgaggacgaaacagcctctacaaataattttgtttaatactagagattaaagaggagaaactatatatgaaagcaattttcgtactgaaacatcttaatcatgctaaggaggttttctaatgagcaaaggagaagaacttttcactggagttgtgccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggt…
encoding: naseq.html

https://synbiohub.org/public/SEGA/SEGA017_seq/1
type: Sequence
persistent identity: SEGA017 seq
display id: SEGA017_seq
version: 1
owned by: carbay
owned by: morno
elements: gtgcatggtctgcaggcgctgccgagccgtattgagcagatgctgtctcaggacaaacgcattgaagcgctggcagaagatttctctgacaaacatcacgcgctgttcctgggccgtggcgatcagtacccaatcgcgctggaaggcgcattgaagttgaaagagatctcttacattcacgctgaagcctacgctgctggcgaactgaaacacggtccgctggcgctaattgatgccgatatgccggttattgttgttgcaccgaacaacgaattgctggaaaaactgaaatccaacattgaagaagttcgcgcgcgtggcggtcagttgtatgtcttcgccgatcaggatgcgggttttgtaagtagcgataacatgcacatcatcgagatgccgcatgtggaagaggtgattgcaccgatcttctacaccgttccgctgcagctgctggcttaccatgtcgcgctgatcaaaggcaccgacgttgaccagccgcgtaacctggcaaaatcggttacggttgagtaataaatggatctcggtaccaaattccagaaaagagacgctttcgagcgtcttttttcgttttggtccgcgttgcgcttgacggctagctcagtcctaggtacagtgctagctactagagattaaagaggagaaacggacgatgaagtacctgctgccgaccgctgctgctggtctgctgctcctcgctgcccagccggcgatggcccgtaaaggcgaagagctgttcactggtgtcgtccctattctggtggaactggatggtgatgtcaacggtcataagttttccgtgcgtggcgagggtgaaggtgacgcaactaatggtaaactgacgctgaagttcatctgtactactggtaaactgccggtaccttggccgactctggtaacgacgctgacttatggtgttcagtgctttgctcgttatccggaccacatgaagcagcatgacttcttcaagtccgccatgccggaaggctatgtgcaggaacgcacgatttcctttaaggatgacggcacgtacaaaacgcgtgcggaagtgaaatttgaaggcgataccctggtaaaccgcattgagctgaaaggcattgactttaaagaagacggcaatatcctgggccataagctggaatacaattttaacagccacaatgtttacatcaccgccgataaacaaaaaaatggcattaaagcgaattttaaaattcgccacaacgtggaggatggcagcgtgcagctggctgatcactaccagcaaaacactccaatcggtgatggtcctgttctgctgccagacaatcactatctgagcacgcaaagcgttctgtctaaagatccgaacgagaaacgcgatcacatggttctgctggagttcgtaaccgcagcgggcatcacgcatggtatggatgaactgtacaaatgaccaattattgaaggccgctaacgcggcctttttttgtttctggtctcccaaaaatttatttgcttattaatcatccggctcgtataatgtgtggagggcccaagttcacttaaaaaggagaatagttatgaaatcgaacaatgcgctcatcgtcatcctcggcaccgtcaccctggatgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagcatcgccagtcactatggcgtgctgctagcgctatatgcgttgatgcaatttctatgcgcacccgttctcggagcactgtccgaccgctttggccgccgcccagtcctgctcgcttcgctacttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggattctctacgccggacgcatcgtggccggcatcaccggcgccacaggtgcggttgctggcgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtg…
encoding: naseq.html

https://synbiohub.org/public/SEGA/SEGA038_seq/1
type: Sequence
persistent identity: SEGA038 seq
display id: SEGA038_seq
version: 1
owned by: carbay
owned by: morno
elements: gtgcatggtctgcaggcgctgccgagccgtattgagcagatgctgtctcaggacaaacgcattgaagcgctggcagaagatttctctgacaaacatcacgcgctgttcctgggccgtggcgatcagtacccaatcgcgctggaaggcgcattgaagttgaaagagatctcttacattcacgctgaagcctacgctgctggcgaactgaaacacggtccgctggcgctaattgatgccgatatgccggttattgttgttgcaccgaacaacgaattgctggaaaaactgaaatccaacattgaagaagttcgcgcgcgtggcggtcagttgtatgtcttcgccgatcaggatgcgggttttgtaagtagcgataacatgcacatcatcgagatgccgcatgtggaagaggtgattgcaccgatcttctacaccgttccgctgcagctgctggcttaccatgtcgcgctgatcaaaggcaccgacgttgaccagccgcgtaacctggcaaaatcggttacggttgagtaataaatggatctcggtaccaaattccagaaaagagacgctttcgagcgtcttttttcgttttggtccgcgttgcgcttaatctttctgcgaattgagatgacgccactggctgggcgtcatcccggtttcccgggtaaacaccaccgaaaaatagttactatcttcaaagccacattcggtcgaaatatcactgattaacaggcggctatgctggagaagatattgcgcatgacacactctgacctgtcgcagatattgattgatggtcattccagtctgctggcgaaattgctgacgcaaaacgcgctcactgcacgatgcctcatcacaaaatttatccagcgcaaagggacttttcaggctagccgccagccgggtaatcagcttatccagcaacgtttcgctggatgttggcggcaacgaatcactggtgtaacgatggcgattcagcaacatcaccaactgcccgaacagcaactcagccatttcgttagcaaacggcacatgctgactactttcatgctcaagctgaccaataacctgccgcgcctgcgccatccccatgctacctaagcgccagtgtggttgccctgcgctggcgttaaatcccggaatcgccccctgccagtcaagattcagcttcagacgctccgggcaataaataatattctgcaaaaccagatcgttaacggaagcgtaggagtgtttatcatcagcatgaatgtaaaagagatcgccacgggtaatgcgataagggcgatcgttgagtacatgcaggccattaccgcgccagacaatcaccagctcacaaaaatcatgtgtatgttcagcaaagacatcttgcggataacggtcagccacagcgactgcctgctggtcgctggcaaaaaaatcatctttgagaagttttaactgatgcgccaccgtggctacctcggccagagaacgaagttgattattcgcaatatggcgtacaaatacgttgagaagattcgcgttattgcagaaagccatcccgtccctggcgaatatcacgcggtgaccagttaaactctcggcgaaaaagcgtcgaaaagtggttactgtcgctgaatccacagcgataggcgatgtcagtaacgctggcctcgctgtggcgtagcagatgtcgggctttcatcagtcgcaggcggttcaggtatcgctgaggcgtcagtcccgtttgctgcttaagctgccgatgtagcgtacgcagtgaaagagaaaattgatccgccacggcatcccaattcacctcatcggcaaaatggtcctccagccaggccagaagcaagttgagacgtgatgcgctgttttccaggttctcctgcaaactgcttttacgcagcaagagcagtaattgcataaacaagatctcgcgactggcggtcgagggtaaatcattttccccttcctgctgttccatctgtgcaaccagctgtcgcacctgctgc…
encoding: naseq.html

https://synbiohub.org/public/SEGA/SEGA012_seq/1
type: Sequence
persistent identity: SEGA012 seq
display id: SEGA012_seq
version: 1
owned by: carbay
owned by: morno
elements: gtgcatggtctgcaggcgctgccgagccgtattgagcagatgctgtctcaggacaaacgcattgaagcgctggcagaagatttctctgacaaacatcacgcgctgttcctgggccgtggcgatcagtacccaatcgcgctggaaggcgcattgaagttgaaagagatctcttacattcacgctgaagcctacgctgctggcgaactgaaacacggtccgctggcgctaattgatgccgatatgccggttattgttgttgcaccgaacaacgaattgctggaaaaactgaaatccaacattgaagaagttcgcgcgcgtggcggtcagttgtatgtcttcgccgatcaggatgcgggttttgtaagtagcgataacatgcacatcatcgagatgccgcatgtggaagaggtgattgcaccgatcttctacaccgttccgctgcagctgctggcttaccatgtcgcgctgatcaaaggcaccgacgttgaccagccgcgtaacctggcaaaatcggttacggttgagtaataaatggatctcggtaccaaattccagaaaagagacgctttcgagcgtcttttttcgttttggtccgcgttgcgcttgacggctagctcagtcctaggtacagtgctagctactagagattaaagaggagaaaaactacatggaaaacctgtacttccagagcaaaggagaagaacttttcactggagttgtgccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgctacatacggaaaactcacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactctgacctatggcgttcaatgcttttcccgttatccggatcacatgaaacggcatgactttttcaagagtgccatgcccgaaggttatgtacaggaacgcactatatctttcaaagatgacgggaactacaagacgcgtgctgaagtcaagtttgaaggtgatacccttgttaatcgtatcgagttaaagggtattgattttaaagaagatggaaacattctcggacacaaactagagtacaactataactcacacaatgtatacatcacggcagacaaacaaaagaatggaatcaaagctaacttcaaaattcgccacaacattgaagatggttccgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtcgacacaatctgccctttcgaaagatcccaacgaaaagcgtgaccacatggtccttcttgagtttgtaactgctgctgggattacacatggcatggatgagctctacaaatgaccaattattgaaggccgctaacgcggcctttttttgtttctggtctcccaaaaatttatttgcttattaatcatccggctcgtataatgtgtggagggcccaagttcacttaaaaaggagaatagttatgaaatcgaacaatgcgctcatcgtcatcctcggcaccgtcaccctggatgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagcatcgccagtcactatggcgtgctgctagcgctatatgcgttgatgcaatttctatgcgcacccgttctcggagcactgtccgaccgctttggccgccgcccagtcctgctcgcttcgctacttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggattctctacgccggacgcatcgtggccggcatcaccggcgccacaggtgcggttgctggcgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggcgccatc…
encoding: naseq.html

https://synbiohub.org/public/SEGA/SEGA069_seq/1
type: Sequence
persistent identity: SEGA069 seq
display id: SEGA069_seq
version: 1
owned by: carbay
owned by: morno
elements: gtgcatggtctgcaggcgctgccgagccgtattgagcagatgctgtctcaggacaaacgcattgaagcgctggcagaagatttctctgacaaacatcacgcgctgttcctgggccgtggcgatcagtacccaatcgcgctggaaggcgcattgaagttgaaagagatctcttacattcacgctgaagcctacgctgctggcgaactgaaacacggtccgctggcgctaattgatgccgatatgccggttattgttgttgcaccgaacaacgaattgctggaaaaactgaaatccaacattgaagaagttcgcgcgcgtggcggtcagttgtatgtcttcgccgatcaggatgcgggttttgtaagtagcgataacatgcacatcatcgagatgccgcatgtggaagaggtgattgcaccgatcttctacaccgttccgctgcagctgctggcttaccatgtcgcgctgatcaaaggcaccgacgttgaccagccgcgtaacctggcaaaatcggttacggttgagtaataaatggatctcggtaccaaattccagaaaagagacgctttcgagcgtcttttttcgttttggtccgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgccagggtggtttttcttttcaccagtgagacgggcaacagctgattgcccttcaccgcctggccctgagagagttgcagcaagcggtccacgctggtttgccccagcaggcgaaaatcctgtttgatggtggttaacggcgggatataacatgagctgtcttcggtatcgtcgtatcccactaccgagatgtccgcaccaacgcgcagcccggactcggtaatggcgcgcattgcgcccagcgccatctgatcgttggcaaccagcatcgcagtgggaacgatgccctcattcagcatttgcatggtttgttgaaaaccggacatggcactccagtcgccttcccgttccgctatcggctgaatttgattgcgagtgagatatttatgccagccagccagacgcagacgcgccgagacagaacttaatgggcccgctaacagcgcgatttgctggtgacccaatgcgaccagatgctccacgcccagtcgcgtaccgtcttcatgggagaaaataatactgttgatgggtgtctggtcagagacatcaagaaataacgccggaacattagtgcaggcagcttccacagcaatggcatcctggtcatccagcggatagttaatgatcagcccactgacgcgttgcgcgagaagattgtgcaccgccgctttacaggcttcgacgccgcttcgttctaccatcgacaccaccacgctggcacccagttgatcggcgcgagatttaatcgccgcgacaatttgcgacggcgcgtgcagggccagactggaggtggcaacgccaatcagcaacgactgtttgcccgccagttgttgtgccacgcggttgggaatgtaattcagctccgccatcgccgcttccactttttcccgcgttttcgcagaaacgtggctggcctggttcaccacgcgggaaacggtctgataagagacaccggcatactctgcgacatcgtataacgttactggtttcacattcaccaccctgaattgactctcttccgggcgctatcatgccataccgcgaaaggttttgcgccattcgatggtgtcggatcgagatcgatctcgatcccgcgaaattaatacgactcactataggggaattgtgagcggataacaattcctactagagattaaagaggagaaatcaatgatgaagtacctgctgccgaccgctgctgctggtctgctgctcctcgctgcccagccggcgatggcccgtaaaggcgaagagctgttcactggtgtcgtccctattctggtggaactggatggtgatgtcaacg…
encoding: naseq.html

https://synbiohub.org/public/SEGA/SEGA086_seq/1
type: Sequence
persistent identity: SEGA086 seq
display id: SEGA086_seq
version: 1
owned by: carbay
owned by: morno
elements: gtgcatggtctgcaggcgctgccgagccgtattgagcagatgctgtctcaggacaaacgcattgaagcgctggcagaagatttctctgacaaacatcacgcgctgttcctgggccgtggcgatcagtacccaatcgcgctggaaggcgcattgaagttgaaagagatctcttacattcacgctgaagcctacgctgctggcgaactgaaacacggtccgctggcgctaattgatgccgatatgccggttattgttgttgcaccgaacaacgaattgctggaaaaactgaaatccaacattgaagaagttcgcgcgcgtggcggtcagttgtatgtcttcgccgatcaggatgcgggttttgtaagtagcgataacatgcacatcatcgagatgccgcatgtggaagaggtgattgcaccgatcttctacaccgttccgctgcagctgctggcttaccatgtcgcgctgatcaaaggcaccgacgttgaccagccgcgtaacctggcaaaatcggttacggttgagtaataaatggatctcggtaccaaattccagaaaagagacgctttcgagcgtcttttttcgttttggtccaccgtattcatcagcactgtcctgctccttgtgatggtttacaaacgtaaaaagtctctttaatacctgtttttgcttcatattgttcagcgacagcttgctgtacggcaggcaccagctcttccgggatcagcgcgacgatacagccgccaaatccgccgccggtcatgcgtacgccacctttgtcgccaatcacagctttgacgatttctaccagagtgtcaatttgcggcacggtgatttcgaaatcatcgcgcatagaggcatgagactccgccatcaactcgcccatacgtttcaggtcgccttgctccagcgcgctggcagcttcaacggtgcgggcgttttcagtcagtatatgacgcacgcgttttgccacgatcgggtccagttcatgcgcaacagcgttgaactcttcaatggtgacatcacgcagggctggctgctggaagaaacgcgcaccggtttcgcactgttcacgacgggtgttgtattcgctgccaaccagggtacgtttgaagttactgttgatgatgacgacagccacacctttgggcatggaaactgctttggtccccagtgagcggcaatcgatcagcaaggcatgatctttcttgccgagcgcggaaattagctgatccatgatcccgcagttacagcctacaaactggttttctgcttcctgaccgttaagcgcgatttgtgcgccgtccagcggcagatgataaagctgctgcaatacggttccgaccgcgacttccagtgaagcggaagaacttaacccggcaccctgcggcacattgccgctgatcaccatgtccacgccgccgaagctgttgttacgcagttgcagatgtttcaccacgccacgaacgtagttagcccattgatagttttcatgtgcgacaatgggcgcatcgagggaaaactcgtcgagctgattttcataatcggctgccatcacgcgaactttacggtcatcgcgtggtgcacaactgatcacggtttgataatcaatcgcgcagggcagaacgaaaccgtcgttgtagtcggtgtgttcaccaatcaaattcacgcggccaggcgcctgaatggtgtgagtggcagggtagccaaatgcgttggcaaacagagattgtgttttttctttcagactcatgattgcctcctgggttagttcctcaccttgtcgtattatactatgccgatatactatgccgatgattaattgtcaacctttaatgaattataaaacgaaaggctcagtcgaaagactgggcctttcgttttatgcgttgcgcttaatctttctgcgaattgagatgacgccactggctgggcgtcatcccggtttcccgggtaaacaccaccgaaaaatagttactatcttcaaagccacattcggt…
encoding: naseq.html

https://synbiohub.org/public/SEGA/SEGA037_seq/1
type: Sequence
persistent identity: SEGA037 seq
display id: SEGA037_seq
version: 1
owned by: carbay
owned by: morno
elements: gtgcatggtctgcaggcgctgccgagccgtattgagcagatgctgtctcaggacaaacgcattgaagcgctggcagaagatttctctgacaaacatcacgcgctgttcctgggccgtggcgatcagtacccaatcgcgctggaaggcgcattgaagttgaaagagatctcttacattcacgctgaagcctacgctgctggcgaactgaaacacggtccgctggcgctaattgatgccgatatgccggttattgttgttgcaccgaacaacgaattgctggaaaaactgaaatccaacattgaagaagttcgcgcgcgtggcggtcagttgtatgtcttcgccgatcaggatgcgggttttgtaagtagcgataacatgcacatcatcgagatgccgcatgtggaagaggtgattgcaccgatcttctacaccgttccgctgcagctgctggcttaccatgtcgcgctgatcaaaggcaccgacgttgaccagccgcgtaacctggcaaaatcggttacggttgagtaataaatggatctcggtaccaaattccagaaaagagacgctttcgagcgtcttttttcgttttggtccgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgccagggtggtttttcttttcaccagtgagacgggcaacagctgattgcccttcaccgcctggccctgagagagttgcagcaagcggtccacgctggtttgccccagcaggcgaaaatcctgtttgatggtggttaacggcgggatataacatgagctgtcttcggtatcgtcgtatcccactaccgagatgtccgcaccaacgcgcagcccggactcggtaatggcgcgcattgcgcccagcgccatctgatcgttggcaaccagcatcgcagtgggaacgatgccctcattcagcatttgcatggtttgttgaaaaccggacatggcactccagtcgccttcccgttccgctatcggctgaatttgattgcgagtgagatatttatgccagccagccagacgcagacgcgccgagacagaacttaatgggcccgctaacagcgcgatttgctggtgacccaatgcgaccagatgctccacgcccagtcgcgtaccgtcttcatgggagaaaataatactgttgatgggtgtctggtcagagacatcaagaaataacgccggaacattagtgcaggcagcttccacagcaatggcatcctggtcatccagcggatagttaatgatcagcccactgacgcgttgcgcgagaagattgtgcaccgccgctttacaggcttcgacgccgcttcgttctaccatcgacaccaccacgctggcacccagttgatcggcgcgagatttaatcgccgcgacaatttgcgacggcgcgtgcagggccagactggaggtggcaacgccaatcagcaacgactgtttgcccgccagttgttgtgccacgcggttgggaatgtaattcagctccgccatcgccgcttccactttttcccgcgttttcgcagaaacgtggctggcctggttcaccacgcgggaaacggtctgataagagacaccggcatactctgcgacatcgtataacgttactggtttcacattcaccaccctgaattgactctcttccgggcgctatcatgccataccgcgaaaggttttgcgccattcgatggtgtcggatcgagatcgatctcgatcccgcgaaatttgacaattaatcatccggctcgtataatggaattgtgagcggataacaattcctactagagattaaagaggagaaaggggctatgaaaaagagaggggcgtttttagggctgttgttggtttctgcctgcgcatcagttttcgctgcgaataacgaaacgagcaagagcgtcaccttcccgaagtgcgaggatctggacgcagcg…
encoding: naseq.html

https://synbiohub.org/public/SEGA/SEGA027_seq/1
type: Sequence
persistent identity: SEGA027 seq
display id: SEGA027_seq
version: 1
owned by: carbay
owned by: morno
elements: gtgcatggtctgcaggcgctgccgagccgtattgagcagatgctgtctcaggacaaacgcattgaagcgctggcagaagatttctctgacaaacatcacgcgctgttcctgggccgtggcgatcagtacccaatcgcgctggaaggcgcattgaagttgaaagagatctcttacattcacgctgaagcctacgctgctggcgaactgaaacacggtccgctggcgctaattgatgccgatatgccggttattgttgttgcaccgaacaacgaattgctggaaaaactgaaatccaacattgaagaagttcgcgcgcgtggcggtcagttgtatgtcttcgccgatcaggatgcgggttttgtaagtagcgataacatgcacatcatcgagatgccgcatgtggaagaggtgattgcaccgatcttctacaccgttccgctgcagctgctggcttaccatgtcgcgctgatcaaaggcaccgacgttgaccagccgcgtaacctggcaaaatcggttacggttgagtaataaatggatctcggtaccaaattccagaaaagagacgctttcgagcgtcttttttcgttttggtccgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgccagggtggtttttcttttcaccagtgagacgggcaacagctgattgcccttcaccgcctggccctgagagagttgcagcaagcggtccacgctggtttgccccagcaggcgaaaatcctgtttgatggtggttaacggcgggatataacatgagctgtcttcggtatcgtcgtatcccactaccgagatgtccgcaccaacgcgcagcccggactcggtaatggcgcgcattgcgcccagcgccatctgatcgttggcaaccagcatcgcagtgggaacgatgccctcattcagcatttgcatggtttgttgaaaaccggacatggcactccagtcgccttcccgttccgctatcggctgaatttgattgcgagtgagatatttatgccagccagccagacgcagacgcgccgagacagaacttaatgggcccgctaacagcgcgatttgctggtgacccaatgcgaccagatgctccacgcccagtcgcgtaccgtcttcatgggagaaaataatactgttgatgggtgtctggtcagagacatcaagaaataacgccggaacattagtgcaggcagcttccacagcaatggcatcctggtcatccagcggatagttaatgatcagcccactgacgcgttgcgcgagaagattgtgcaccgccgctttacaggcttcgacgccgcttcgttctaccatcgacaccaccacgctggcacccagttgatcggcgcgagatttaatcgccgcgacaatttgcgacggcgcgtgcagggccagactggaggtggcaacgccaatcagcaacgactgtttgcccgccagttgttgtgccacgcggttgggaatgtaattcagctccgccatcgccgcttccactttttcccgcgttttcgcagaaacgtggctggcctggttcaccacgcgggaaacggtctgataagagacaccggcatactctgcgacatcgtataacgttactggtttcacattcaccaccctgaattgactctcttccgggcgctatcatgccataccgcgaaaggttttgcgccattcgatggtgtcggatcgagatcgatctcgatcccgcgaaatttgacaattaatcatccggctcgtataatggaattgtgagcggataacaattcctactagagattaaagaggagaaaaactacatggaaaacctgtacttccagagcaaaggagaagaacttttcactggagttgtgccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggt…
encoding: naseq.html

https://synbiohub.org/public/SEGA/SEGA079_seq/1
type: Sequence
persistent identity: SEGA079 seq
display id: SEGA079_seq
version: 1
owned by: carbay
owned by: morno
elements: gtgcatggtctgcaggcgctgccgagccgtattgagcagatgctgtctcaggacaaacgcattgaagcgctggcagaagatttctctgacaaacatcacgcgctgttcctgggccgtggcgatcagtacccaatcgcgctggaaggcgcattgaagttgaaagagatctcttacattcacgctgaagcctacgctgctggcgaactgaaacacggtccgctggcgctaattgatgccgatatgccggttattgttgttgcaccgaacaacgaattgctggaaaaactgaaatccaacattgaagaagttcgcgcgcgtggcggtcagttgtatgtcttcgccgatcaggatgcgggttttgtaagtagcgataacatgcacatcatcgagatgccgcatgtggaagaggtgattgcaccgatcttctacaccgttccgctgcagctgctggcttaccatgtcgcgctgatcaaaggcaccgacgttgaccagccgcgtaacctggcaaaatcggttacggttgagtaataaatggatctcggtaccaaattccagaaaagagacgctttcgagcgtcttttttcgttttggtccaccgtattcatcagcactgtcctgctccttgtgatggtttacaaacgtaaagagtctctttaatacctgtttttgcttcatattgttcagcgacagcttgctgtacggcaggcaccagctcttccgggatcagcgcgacgatacagccgccaaatccgccgccggtcatgcgtacgccacctttgtcgccaatcacagctttgacgatttctaccagagtgtcaatttgcggcacggtgatttcgaaatcatcgcgcatagaggcatgagactccgccatcaactcgcccatacgtttcaggtcgccttgctccagcgcgctggcagcttcaacggtgcgggcgttttcagtcagtatatgacgcacgcgttttgccacgatcgggtccagttcatgcgcaacagcgttgaactcttcaatggtgacatcacgcagggctggctgctggaagaaacgcgcaccggtttcgcactgttcacgacgggtgttgtattcgctgccaaccagggtacgtttgaagttactgttgatgatgacgacagccacacctttgggcatggaaactgctttggtccccagtgagcggcaatcgatcagcaaggcatgatctttcttgccgagcgcggaaattagctgatccatgatcccgcagttacagcctacaaactggttttctgcttcctgaccgttaagcgcgatttgtgcgccgtccagcggcagatgataaagctgctgcaatacggttccgaccgcgacttccagtgaagcggaagaacttaacccggcaccctgcggcacattgccgctgatcaccatgtccacgccgccgaagctgttgttacgcagttgcagatgtttcaccacgccacgaacgtagttagcccattgatagttttcatgtgcgacaatgggcgcatcgagggaaaactcgtcgagctgattttcataatcggctgccatcacgcgaactttacggtcatcgcgtggtgcacaactgatcacggtttgataatcaatcgcgcagggcagaacgaaaccgtcgttgtagtcggtgtgttcaccaatcaaattcacgcggccaggcgcctgaatggtgtgagtggcagggtagccaaatgcgttggcaaacagagattgtgttttttctttcagactcatgattgcctcctgggttagttcctcaccttgtcgtattatactatgccgatatactatgccgatgattaattgtcaacctttaatgaattataaaacgaaaggctcagtcgaaagactgggcctttcgttttatgcgttgcgcttaatctttctgcgaattgagatgacgccactggctgggcgtcatcccggtttcccgggtaaacaccaccgaaaaatagttactatcttcaaagccacattcggt…
encoding: naseq.html

https://synbiohub.org/public/SEGA/SEGA085_seq/1
type: Sequence
persistent identity: SEGA085 seq
display id: SEGA085_seq
version: 1
owned by: carbay
owned by: morno
elements: gtgcatggtctgcaggcgctgccgagccgtattgagcagatgctgtctcaggacaaacgcattgaagcgctggcagaagatttctctgacaaacatcacgcgctgttcctgggccgtggcgatcagtacccaatcgcgctggaaggcgcattgaagttgaaagagatctcttacattcacgctgaagcctacgctgctggcgaactgaaacacggtccgctggcgctaattgatgccgatatgccggttattgttgttgcaccgaacaacgaattgctggaaaaactgaaatccaacattgaagaagttcgcgcgcgtggcggtcagttgtatgtcttcgccgatcaggatgcgggttttgtaagtagcgataacatgcacatcatcgagatgccgcatgtggaagaggtgattgcaccgatcttctacaccgttccgctgcagctgctggcttaccatgtcgcgctgatcaaaggcaccgacgttgaccagccgcgtaacctggcaaaatcggttacggttgagtaataaatggatctcggtaccaaattccagaaaagagacgctttcgagcgtcttttttcgttttggtccaccgtattcatcagcactgtcctgctccttgtgatggtttacaaacgtaaaaagtctctttaatacctgtttttgcttcatattgttcagcgacagcttgctgtacggcaggcaccagctcttccgggatcagcgcgacgatacagccgccaaatccgccgccggtcatgcgtacgccacctttgtcgccaatcacagctttgacgatttctaccagagtgtcaatttgcggcacggtgatttcgaaatcatcgcgcatagaggcatgagactccgccatcaactcgcccatacgtttcaggtcgccttgctccagcgcgctggcagcttcaacggtgcgggcgttttcagtcagtatatgacgcacgcgttttgccacgatcgggtccagttcatgcgcaacagcgttgaactcttcaatggtgacatcacgcagggctggctgctggaagaaacgcgcaccggtttcgcactgttcacgacgggtgttgtattcgctgccaaccagggtacgtttgaagttactgttgatgatgacgacagccacacctttgggcatggaaactgctttggtccccagtgagcggcaatcgatcagcaaggcatgatctttcttgccgagcgcggaaattagctgatccatgatcccgcagttacagcctacaaactggttttctgcttcctgaccgttaagcgcgatttgtgcgccgtccagcggcagatgataaagctgctgcaatacggttccgaccgcgacttccagtgaagcggaagaacttaacccggcaccctgcggcacattgccgctgatcaccatgtccacgccgccgaagctgttgttacgcagttgcagatgtttcaccacgccacgaacgtagttagcccattgatagttttcatgtgcgacaatgggcgcatcgagggaaaactcgtcgagctgattttcataatcggctgccatcacgcgaactttacggtcatcgcgtggtgcacaactgatcacggtttgataatcaatcgcgcagggcagaacgaaaccgtcgttgtagtcggtgtgttcaccaatcaaattcacgcggccaggcgcctgaatggtgtgagtggcagggtagccaaatgcgttggcaaacagagattgtgttttttctttcagactcatgattgcctcctgggttagttcctcaccttgtcgtattatactatgccgatatactatgccgatgattaattgtcaacctttaatgaattataaaacgaaaggctcagtcgaaagactgggcctttcgttttatgcgttgcgcttaatctttctgcgaattgagatgacgccactggctgggcgtcatcccggtttcccgggtaaacaccaccgaaaaatagttactatcttcaaagccacattcggt…
encoding: naseq.html

https://synbiohub.org/public/SEGA/SEGA082_seq/1
type: Sequence
persistent identity: SEGA082 seq
display id: SEGA082_seq
version: 1
owned by: carbay
owned by: morno
elements: gtgcatggtctgcaggcgctgccgagccgtattgagcagatgctgtctcaggacaaacgcattgaagcgctggcagaagatttctctgacaaacatcacgcgctgttcctgggccgtggcgatcagtacccaatcgcgctggaaggcgcattgaagttgaaagagatctcttacattcacgctgaagcctacgctgctggcgaactgaaacacggtccgctggcgctaattgatgccgatatgccggttattgttgttgcaccgaacaacgaattgctggaaaaactgaaatccaacattgaagaagttcgcgcgcgtggcggtcagttgtatgtcttcgccgatcaggatgcgggttttgtaagtagcgataacatgcacatcatcgagatgccgcatgtggaagaggtgattgcaccgatcttctacaccgttccgctgcagctgctggcttaccatgtcgcgctgatcaaaggcaccgacgttgaccagccgcgtaacctggcaaaatcggttacggttgagtaataaatggatctcggtaccaaattccagaaaagagacgctttcgagcgtcttttttcgttttggtccaccgtattcatcagcactgtcctgctccttgtgatggtttacaaacgtaaaaagtctctttaatacctgtttttgcttcatattgttcagcgacagcttgctgtacggcaggcaccagctcttccgggatcagcgcgacgatacagccgccaaatccgccgccggtcatgcgtacgccacctttgtcgccaatcacagctttgacgatttctaccagagtgtcaatttgcggcacggtgatttcgaaatcatcgcgcatagaggcatgagactccgccatcaactcgcccatacgtttcaggtcgccttgctccagcgcgctggcagcttcaacggtgcgggcgttttcagtcagtatatgacgcacgcgttttgccacgatcgggtccagttcatgcgcaacagcgttgaactcttcaatggtgacatcacgcagggctggctgctggaagaaacgcgcaccggtttcgcactgttcacgacgggtgttgtattcgctgccaaccagggtacgtttgaagttactgttgatgatgacgacagccacacctttgggcatggaaactgctttggtccccagtgagcggcaatcgatcagcaaggcatgatctttcttgccgagcgcggaaattagctgatccatgatcccgcagttacagcctacaaactggttttctgcttcctgaccgttaagcgcgatttgtgcgccgtccagcggcagatgataaagctgctgcaatacggttccgaccgcgacttccagtgaagcggaagaacttaacccggcaccctgcggcacattgccgctgatcaccatgtccacgccgccgaagctgttgttacgcagttgcagatgtttcaccacgccacgaacgtagttagcccattgatagttttcatgtgcgacaatgggcgcatcgagggaaaactcgtcgagctgattttcataatcggctgccatcacgcgaactttacggtcatcgcgtggtgcacaactgatcacggtttgataatcaatcgcgcagggcagaacgaaaccgtcgttgtagtcggtgtgttcaccaatcaaattcacgcggccaggcgcctgaatggtgtgagtggcagggtagccaaatgcgttggcaaacagagattgtgttttttctttcagactcatgattgcctcctgggttagttcctcaccttgtcgtattatactatgccgatatactatgccgatgattaattgtcaacctttaatgaattataaaacgaaaggctcagtcgaaagactgggcctttcgttttatgcgttgcgcttaatctttctgcgaattgagatgacgccactggctgggcgtcatcccggtttcccgggtaaacaccaccgaaaaatagttactatcttcaaagccacattcggt…
encoding: naseq.html

https://synbiohub.org/public/SEGA/SEGA091_seq/1
type: Sequence
persistent identity: SEGA091 seq
display id: SEGA091_seq
version: 1
owned by: carbay
owned by: morno
elements: gtgcatggtctgcaggcgctgccgagccgtattgagcagatgctgtctcaggacaaacgcattgaagcgctggcagaagatttctctgacaaacatcacgcgctgttcctgggccgtggcgatcagtacccaatcgcgctggaaggcgcattgaagttgaaagagatctcttacattcacgctgaagcctacgctgctggcgaactgaaacacggtccgctggcgctaattgatgccgatatgccggttattgttgttgcaccgaacaacgaattgctggaaaaactgaaatccaacattgaagaagttcgcgcgcgtggcggtcagttgtatgtcttcgccgatcaggatgcgggttttgtaagtagcgataacatgcacatcatcgagatgccgcatgtggaagaggtgattgcaccgatcttctacaccgttccgctgcagctgctggcttaccatgtcgcgctgatcaaaggcaccgacgttgaccagccgcgtaacctggcaaaatcggttacggttgagtaataaatggatctcggtaccaaattccagaaaagagacgctttcgagcgtcttttttcgttttggtccaccgtattcatcagcactgtcctgctccttgtgatggtttacaaacgtaaaaagtctctttaatacctgtttttgcttcatattgttcagcgacagcttgctgtacggcaggcaccagctcttccgggatcagcgcgacgatacagccgccaaatccgccgccggtcatgcgtacgccacctttgtcgccaatcacagctttgacgatttctaccagagtgtcaatttgcggcacggtgatttcgaaatcatcgcgcatagaggcatgagactccgccatcaactcgcccatacgtttcaggtcgccttgctccagcgcgctggcagcttcaacggtgcgggcgttttcagtcagtatatgacgcacgcgttttgccacgatcgggtccagttcatgcgcaacagcgttgaactcttcaatggtgacatcacgcagggctggctgctggaagaaacgcgcaccggtttcgcactgttcacgacgggtgttgtattcgctgccaaccagggtacgtttgaagttactgttgatgatgacgacagccacacctttgggcatggaaactgctttggtccccagtgagcggcaatcgatcagcaaggcatgatctttcttgccgagcgcggaaattagctgatccatgatcccgcagttacagcctacaaactggttttctgcttcctgaccgttaagcgcgatttgtgcgccgtccagcggcagatgataaagctgctgcaatacggttccgaccgcgacttccagtgaagcggaagaacttaacccggcaccctgcggcacattgccgctgatcaccatgtccacgccgccgaagctgttgttacgcagttgcagatgtttcaccacgccacgaacgtagttagcccattgatagttttcatgtgcgacaatgggcgcatcgagggaaaactcgtcgagctgattttcataatcggctgccatcacgcgaactttacggtcatcgcgtggtgcacaactgatcacggtttgataatcaatcgcgcagggcagaacgaaaccgtcgttgtagtcggtgtgttcaccaatcaaattcacgcggccaggcgcctgaatggtgtgagtggcagggtagccaaatgcgttggcaaacagagattgtgttttttctttcagactcatgattgcctcctgggttagttcctcaccttgtcgtattatactatgccgatatactatgccgatgattaattgtcaacctttaatgaattataaaacgaaaggctcagtcgaaagactgggcctttcgttttatgcgttgcgcttaatctttctgcgaattgagatgacgccactggctgggcgtcatcccggtttcccgggtaaacaccaccgaaaaatagttactatcttcaaagccacattcggt…
encoding: naseq.html

https://synbiohub.org/public/SEGA/SEGA081_seq/1
type: Sequence
persistent identity: SEGA081 seq
display id: SEGA081_seq
version: 1
owned by: carbay
owned by: morno
elements: gtgcatggtctgcaggcgctgccgagccgtattgagcagatgctgtctcaggacaaacgcattgaagcgctggcagaagatttctctgacaaacatcacgcgctgttcctgggccgtggcgatcagtacccaatcgcgctggaaggcgcattgaagttgaaagagatctcttacattcacgctgaagcctacgctgctggcgaactgaaacacggtccgctggcgctaattgatgccgatatgccggttattgttgttgcaccgaacaacgaattgctggaaaaactgaaatccaacattgaagaagttcgcgcgcgtggcggtcagttgtatgtcttcgccgatcaggatgcgggttttgtaagtagcgataacatgcacatcatcgagatgccgcatgtggaagaggtgattgcaccgatcttctacaccgttccgctgcagctgctggcttaccatgtcgcgctgatcaaaggcaccgacgttgaccagccgcgtaacctggcaaaatcggttacggttgagtaataaatggatctcggtaccaaattccagaaaagagacgctttcgagcgtcttttttcgttttggtccaccgtattcatcagcactgtcctgctccttgtgatggtttacaaacgtaaaaagtctctttaatacctgtttttgcttcatattgttcagcgacagcttgctgtacggcaggcaccagctcttccgggatcagcgcgacgatacagccgccaaatccgccgccggtcatgcgtacgccacctttgtcgccaatcacagctttgacgatttctaccagagtgtcaatttgcggcacggtgatttcgaaatcatcgcgcatagaggcatgagactccgccatcaactcgcccatacgtttcaggtcgccttgctccagcgcgctggcagcttcaacggtgcgggcgttttcagtcagtatatgacgcacgcgttttgccacgatcgggtccagttcatgcgcaacagcgttgaactcttcaatggtgacatcacgcagggctggctgctggaagaaacgcgcaccggtttcgcactgttcacgacgggtgttgtattcgctgccaaccagggtacgtttgaagttactgttgatgatgacgacagccacacctttgggcatggaaactgctttggtccccagtgagcggcaatcgatcagcaaggcatgatctttcttgccgagcgcggaaattagctgatccatgatcccgcagttacagcctacaaactggttttctgcttcctgaccgttaagcgcgatttgtgcgccgtccagcggcagatgataaagctgctgcaatacggttccgaccgcgacttccagtgaagcggaagaacttaacccggcaccctgcggcacattgccgctgatcaccatgtccacgccgccgaagctgttgttacgcagttgcagatgtttcaccacgccacgaacgtagttagcccattgatagttttcatgtgcgacaatgggcgcatcgagggaaaactcgtcgagctgattttcataatcggctgccatcacgcgaactttacggtcatcgcgtggtgcacaactgatcacggtttgataatcaatcgcgcagggcagaacgaaaccgtcgttgtagtcggtgtgttcaccaatcaaattcacgcggccaggcgcctgaatggtgtgagtggcagggtagccaaatgcgttggcaaacagagattgtgttttttctttcagactcatgattgcctcctgggttagttcctcaccttgtcgtattatactatgccgatatactatgccgatgattaattgtcaacctttaatgaattataaaacgaaaggctcagtcgaaagactgggcctttcgttttatgcgttgcgcttaatctttctgcgaattgagatgacgccactggctgggcgtcatcccggtttcccgggtaaacaccaccgaaaaatagttactatcttcaaagccacattcggt…
encoding: naseq.html

https://synbiohub.org/public/SEGA/SEGA022_seq/1
type: Sequence
persistent identity: SEGA022 seq
display id: SEGA022_seq
version: 1
owned by: carbay
owned by: morno
elements: gtgcatggtctgcaggcgctgccgagccgtattgagcagatgctgtctcaggacaaacgcattgaagcgctggcagaagatttctctgacaaacatcacgcgctgttcctgggccgtggcgatcagtacccaatcgcgctggaaggcgcattgaagttgaaagagatctcttacattcacgctgaagcctacgctgctggcgaactgaaacacggtccgctggcgctaattgatgccgatatgccggttattgttgttgcaccgaacaacgaattgctggaaaaactgaaatccaacattgaagaagttcgcgcgcgtggcggtcagttgtatgtcttcgccgatcaggatgcgggttttgtaagtagcgataacatgcacatcatcgagatgccgcatgtggaagaggtgattgcaccgatcttctacaccgttccgctgcagctgctggcttaccatgtcgcgctgatcaaaggcaccgacgttgaccagccgcgtaacctggcaaaatcggttacggttgagtaataaatggatctcggtaccaaattccagaaaagagacgctttcgagcgtcttttttcgttttggtccgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgccagggtggtttttcttttcaccagtgagacgggcaacagctgattgcccttcaccgcctggccctgagagagttgcagcaagcggtccacgctggtttgccccagcaggcgaaaatcctgtttgatggtggttaacggcgggatataacatgagctgtcttcggtatcgtcgtatcccactaccgagatgtccgcaccaacgcgcagcccggactcggtaatggcgcgcattgcgcccagcgccatctgatcgttggcaaccagcatcgcagtgggaacgatgccctcattcagcatttgcatggtttgttgaaaaccggacatggcactccagtcgccttcccgttccgctatcggctgaatttgattgcgagtgagatatttatgccagccagccagacgcagacgcgccgagacagaacttaatgggcccgctaacagcgcgatttgctggtgacccaatgcgaccagatgctccacgcccagtcgcgtaccgtcttcatgggagaaaataatactgttgatgggtgtctggtcagagacatcaagaaataacgccggaacattagtgcaggcagcttccacagcaatggcatcctggtcatccagcggatagttaatgatcagcccactgacgcgttgcgcgagaagattgtgcaccgccgctttacaggcttcgacgccgcttcgttctaccatcgacaccaccacgctggcacccagttgatcggcgcgagatttaatcgccgcgacaatttgcgacggcgcgtgcagggccagactggaggtggcaacgccaatcagcaacgactgtttgcccgccagttgttgtgccacgcggttgggaatgtaattcagctccgccatcgccgcttccactttttcccgcgttttcgcagaaacgtggctggcctggttcaccacgcgggaaacggtctgataagagacaccggcatactctgcgacatcgtataacgttactggtttcacattcaccaccctgaattgactctcttccgggcgctatcatgccataccgcgaaaggttttgcgccattcgatggtgtcggatcgagatcgatctcgatcccgcgaaatttgacaattaatcatccggctcgtataatggaattgtgagcggataacaattcctactagagattaaagaggagaaactatatatgaaagcaattttcgtactgaaacatcttaatcatgctaaggaggttttctaatgagcaaaggagaagaacttttcactggagttgtgccaattcttgttgaattagatggtgatgttaatg…
encoding: naseq.html

https://synbiohub.org/public/SEGA/SEGA013_seq/1
type: Sequence
persistent identity: SEGA013 seq
display id: SEGA013_seq
version: 1
owned by: carbay
owned by: morno
elements: gtgcatggtctgcaggcgctgccgagccgtattgagcagatgctgtctcaggacaaacgcattgaagcgctggcagaagatttctctgacaaacatcacgcgctgttcctgggccgtggcgatcagtacccaatcgcgctggaaggcgcattgaagttgaaagagatctcttacattcacgctgaagcctacgctgctggcgaactgaaacacggtccgctggcgctaattgatgccgatatgccggttattgttgttgcaccgaacaacgaattgctggaaaaactgaaatccaacattgaagaagttcgcgcgcgtggcggtcagttgtatgtcttcgccgatcaggatgcgggttttgtaagtagcgataacatgcacatcatcgagatgccgcatgtggaagaggtgattgcaccgatcttctacaccgttccgctgcagctgctggcttaccatgtcgcgctgatcaaaggcaccgacgttgaccagccgcgtaacctggcaaaatcggttacggttgagtaataaatggatctcggtaccaaattccagaaaagagacgctttcgagcgtcttttttcgttttggtccgcgttgcgcttgacggctagctcagtcctaggtacagtgctagctactagagattaaagaggagaaaatatcgatggagaacctgtacttccagagcaaaggagaagaacttttcactggagttgtgccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgctacatacggaaaactcacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactctgacctatggcgttcaatgcttttcccgttatccggatcacatgaaacggcatgactttttcaagagtgccatgcccgaaggttatgtacaggaacgcactatatctttcaaagatgacgggaactacaagacgcgtgctgaagtcaagtttgaaggtgatacccttgttaatcgtatcgagttaaagggtattgattttaaagaagatggaaacattctcggacacaaactagagtacaactataactcacacaatgtatacatcacggcagacaaacaaaagaatggaatcaaagctaacttcaaaattcgccacaacattgaagatggttccgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtcgacacaatctaccctttcgaaagatcccaacgaaaagcgtgaccacatggtccttcttgagtttgtaactgctgctgggattacacatggcatggatgagctctacaaatgaccaattattgaaggccgctaacgcggcctttttttgtttctggtctcccaaaaatttatttgcttattaatcatccggctcgtataatgtgtggagggcccaagttcacttaaaaaggagaatagttatgaaatcgaacaatgcgctcatcgtcatcctcggcaccgtcaccctggatgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagcatcgccagtcactatggcgtgctgctagcgctatatgcgttgatgcaatttctatgcgcacccgttctcggagcactgtccgaccgctttggccgccgcccagtcctgctcgcttcgctacttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggattctctacgccggacgcatcgtggccggcatcaccggcgccacaggtgcggttgctggcgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggcgccatc…
encoding: naseq.html

https://synbiohub.org/public/SEGA/SEGA094_seq/1
type: Sequence
persistent identity: SEGA094 seq
display id: SEGA094_seq
version: 1
owned by: carbay
owned by: morno
elements: gtgcatggtctgcaggcgctgccgagccgtattgagcagatgctgtctcaggacaaacgcattgaagcgctggcagaagatttctctgacaaacatcacgcgctgttcctgggccgtggcgatcagtacccaatcgcgctggaaggcgcattgaagttgaaagagatctcttacattcacgctgaagcctacgctgctggcgaactgaaacacggtccgctggcgctaattgatgccgatatgccggttattgttgttgcaccgaacaacgaattgctggaaaaactgaaatccaacattgaagaagttcgcgcgcgtggcggtcagttgtatgtcttcgccgatcaggatgcgggttttgtaagtagcgataacatgcacatcatcgagatgccgcatgtggaagaggtgattgcaccgatcttctacaccgttccgctgcagctgctggcttaccatgtcgcgctgatcaaaggcaccgacgttgaccagccgcgtaacctggcaaaatcggttacggttgagtaataaatggatctcggtaccaaattccagaaaagagacgctttcgagcgtcttttttcgttttggtccaccgtattcatcagcactgtcctgctccttgtgatggtttacaaacgtaaaaagtctctttaatacctgtttttgcttcatattgttcagcgacagcttgctgtacggcaggcaccagctcttccgggatcagcgcgacgatacagccgccaaatccgccgccggtcatgcgtacgccacctttgtcgccaatcacagctttgacgatttctaccagagtgtcaatttgcggcacggtgatttcgaaatcatcgcgcatagaggcatgagactccgccatcaactcgcccatacgtttcaggtcgccttgctccagcgcgctggcagcttcaacggtgcgggcgttttcagtcagtatatgacgcacgcgttttgccacgatcgggtccagttcatgcgcaacagcgttgaactcttcaatggtgacatcacgcagggctggctgctggaagaaacgcgcaccggtttcgcactgttcacgacgggtgttgtattcgctgccaaccagggtacgtttgaagttactgttgatgatgacgacagccacacctttgggcatggaaactgctttggtccccagtgagcggcaatcgatcagcaaggcatgatctttcttgccgagcgcggaaattagctgatccatgatcccgcagttacagcctacaaactggttttctgcttcctgaccgttaagcgcgatttgtgcgccgtccagcggcagatgataaagctgctgcaatacggttccgaccgcgacttccagtgaagcggaagaacttaacccggcaccctgcggcacattgccgctgatcaccatgtccacgccgccgaagctgttgttacgcagttgcagatgtttcaccacgccacgaacgtagttagcccattgatagttttcatgtgcgacaatgggcgcatcgagggaaaactcgtcgagctgattttcataatcggctgccatcacgcgaactttacggtcatcgcgtggtgcacaactgatcacggtttgataatcaatcgcgcagggcagaacgaaaccgtcgttgtagtcggtgtgttcaccaatcaaattcacgcggccaggcgcctgaatggtgtgagtggcagggtagccaaatgcgttggcaaacagagattgtgttttttctttcagactcatgattgcctcctgggttagttcctcaccttgtcgtattatactatgccgatatactatgccgatgattaattgtcaacctttaatgaattataaaacgaaaggctcagtcgaaagactgggcctttcgttttatgcgttgcgcttaatctttctgcgaattgagatgacgccactggctgggcgtcatcccggtttcccgggtaaacaccaccgaaaaatagttactatcttcaaagccacattcggt…
encoding: naseq.html

https://synbiohub.org/public/SEGA/SEGA010_seq/1
type: Sequence
persistent identity: SEGA010 seq
display id: SEGA010_seq
version: 1
owned by: carbay
owned by: morno
elements: gtgcatggtctgcaggcgctgccgagccgtattgagcagatgctgtctcaggacaaacgcattgaagcgctggcagaagatttctctgacaaacatcacgcgctgttcctgggccgtggcgatcagtacccaatcgcgctggaaggcgcattgaagttgaaagagatctcttacattcacgctgaagcctacgctgctggcgaactgaaacacggtccgctggcgctaattgatgccgatatgccggttattgttgttgcaccgaacaacgaattgctggaaaaactgaaatccaacattgaagaagttcgcgcgcgtggcggtcagttgtatgtcttcgccgatcaggatgcgggttttgtaagtagcgataacatgcacatcatcgagatgccgcatgtggaagaggtgattgcaccgatcttctacaccgttccgctgcagctgctggcttaccatgtcgcgctgatcaaaggcaccgacgttgaccagccgcgtaacctggcaaaatcggttacggttgagtaataaatggatctcggtaccaaattccagaaaagagacgctttcgagcgtcttttttcgttttggtccgcgttgcgcttgacggctagctcagtcctaggtacagtgctagctactagagattaaagaggagaaacattgtatgcagatattcgtgaaaacactgaccggcaaaaccattaccctggaggtggagcctggcagcgcccatcatcatcaccatcaccatcaccatcatgctggtagcagcgatacgatcgaaaacgtcaaagccaaaattcaggacaaagagggcattccgcctgatcaacaacgtctgatcttcgccggaaaacagctggaggatggccgtacactgagtgactataacattcaagagaaatcgacactggaactggttctggagctgcagaatgaaacccagtcagcaacttctgacgcttctggcggtagcaaaggagaagaacttttcactggagttgtgccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgctacatacggaaaactcacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactctgacctatggcgttcaatgcttttcccgttatccggatcacatgaaacggcatgactttttcaagagtgccatgcccgaaggttatgtacaggaacgcactatatctttcaaagatgacgggaactacaagacgcgtgctgaagtcaagtttgaaggtgatacccttgttaatcgtatcgagttaaagggtattgattttaaagaagatggaaacattctcggacacaaactagagtacaactataactcacacaatgtatacatcacggcagacaaacaaaagaatggaatcaaagctaacttcaaaattcgccacaacattgaagatggttccgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtcgacacaatctgccctttcgaaagatcccaacgaaaagcgtgaccacatggtccttcttgagtttgtaactgctgctgggattacacatggcatggatgagctctacaaatgaccaattattgaaggccgctaacgcggcctttttttgtttctggtctcccaaaaatttatttgcttattaatcatccggctcgtataatgtgtggagggcccaagttcacttaaaaaggagaatagttatgaaatcgaacaatgcgctcatcgtcatcctcggcaccgtcaccctggatgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagcatcgccagtcactatggcgtgctgctagcgctatatgcgttgatgcaatttctatgcgcaccc…
encoding: naseq.html

https://synbiohub.org/public/SEGA/SEGA060_seq/1
type: Sequence
persistent identity: SEGA060 seq
display id: SEGA060_seq
version: 1
owned by: carbay
owned by: morno
elements: gtgcatggtctgcaggcgctgccgagccgtattgagcagatgctgtctcaggacaaacgcattgaagcgctggcagaagatttctctgacaaacatcacgcgctgttcctgggccgtggcgatcagtacccaatcgcgctggaaggcgcattgaagttgaaagagatctcttacattcacgctgaagcctacgctgctggcgaactgaaacacggtccgctggcgctaattgatgccgatatgccggttattgttgttgcaccgaacaacgaattgctggaaaaactgaaatccaacattgaagaagttcgcgcgcgtggcggtcagttgtatgtcttcgccgatcaggatgcgggttttgtaagtagcgataacatgcacatcatcgagatgccgcatgtggaagaggtgattgcaccgatcttctacaccgttccgctgcagctgctggcttaccatgtcgcgctgatcaaaggcaccgacgttgaccagccgcgtaacctggcaaaatcggttacggttgagtaataaatggatctcggtaccaaattccagaaaagagacgctttcgagcgtcttttttcgttttggtccgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgccagggtggtttttcttttcaccagtgagacgggcaacagctgattgcccttcaccgcctggccctgagagagttgcagcaagcggtccacgctggtttgccccagcaggcgaaaatcctgtttgatggtggttaacggcgggatataacatgagctgtcttcggtatcgtcgtatcccactaccgagatgtccgcaccaacgcgcagcccggactcggtaatggcgcgcattgcgcccagcgccatctgatcgttggcaaccagcatcgcagtgggaacgatgccctcattcagcatttgcatggtttgttgaaaaccggacatggcactccagtcgccttcccgttccgctatcggctgaatttgattgcgagtgagatatttatgccagccagccagacgcagacgcgccgagacagaacttaatgggcccgctaacagcgcgatttgctggtgacccaatgcgaccagatgctccacgcccagtcgcgtaccgtcttcatgggagaaaataatactgttgatgggtgtctggtcagagacatcaagaaataacgccggaacattagtgcaggcagcttccacagcaatggcatcctggtcatccagcggatagttaatgatcagcccactgacgcgttgcgcgagaagattgtgcaccgccgctttacaggcttcgacgccgcttcgttctaccatcgacaccaccacgctggcacccagttgatcggcgcgagatttaatcgccgcgacaatttgcgacggcgcgtgcagggccagactggaggtggcaacgccaatcagcaacgactgtttgcccgccagttgttgtgccacgcggttgggaatgtaattcagctccgccatcgccgcttccactttttcccgcgttttcgcagaaacgtggctggcctggttcaccacgcgggaaacggtctgataagagacaccggcatactctgcgacatcgtataacgttactggtttcacattcaccaccctgaattgactctcttccgggcgctatcatgccataccgcgaaaggttttgcgccattcgatggtgtcggatcgagatcgatctcgatcccgcgaaattaatacgactcactataggggaattgtgagcggataacaattcctactagagattaaagaggagaaaggctggatgcaaatattcgtgaaaacactgaccggcaaaaccattaccctggaggtggagcctggcagcgcccatcatcatcaccatcaccatcaccatcatgctggtagcagcgatacgatcgaaaacgtcaaagcca…
encoding: naseq.html

https://synbiohub.org/public/SEGA/SEGA104_seq/1
type: Sequence
persistent identity: SEGA104 seq
display id: SEGA104_seq
version: 1
owned by: carbay
owned by: morno
elements: gtgcatggtctgcaggcgctgccgagccgtattgagcagatgctgtctcaggacaaacgcattgaagcgctggcagaagatttctctgacaaacatcacgcgctgttcctgggccgtggcgatcagtacccaatcgcgctggaaggcgcattgaagttgaaagagatctcttacattcacgctgaagcctacgctgctggcgaactgaaacacggtccgctggcgctaattgatgccgatatgccggttattgttgttgcaccgaacaacgaattgctggaaaaactgaaatccaacattgaagaagttcgcgcgcgtggcggtcagttgtatgtcttcgccgatcaggatgcgggttttgtaagtagcgataacatgcacatcatcgagatgccgcatgtggaagaggtgattgcaccgatcttctacaccgttccgctgcagctgctggcttaccatgtcgcgctgatcaaaggcaccgacgttgaccagccgcgtaacctggcaaaatcggttacggttgagtaataaatggatctcggtaccaaattccagaaaagagacgctttcgagcgtcttttttcgttttggtccgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgccagggtggtttttcttttcaccagtgagacgggcaacagctgattgcccttcaccgcctggccctgagagagttgcagcaagcggtccacgctggtttgccccagcaggcgaaaatcctgtttgatggtggttaacggcgggatataacatgagctgtcttcggtatcgtcgtatcccactaccgagatgtccgcaccaacgcgcagcccggactcggtaatggcgcgcattgcgcccagcgccatctgatcgttggcaaccagcatcgcagtgggaacgatgccctcattcagcatttgcatggtttgttgaaaaccggacatggcactccagtcgccttcccgttccgctatcggctgaatttgattgcgagtgagatatttatgccagccagccagacgcagacgcgccgagacagaacttaatgggcccgctaacagcgcgatttgctggtgacccaatgcgaccagatgctccacgcccagtcgcgtaccgtcttcatgggagaaaataatactgttgatgggtgtctggtcagagacatcaagaaataacgccggaacattagtgcaggcagcttccacagcaatggcatcctggtcatccagcggatagttaatgatcagcccactgacgcgttgcgcgagaagattgtgcaccgccgctttacaggcttcgacgccgcttcgttctaccatcgacaccaccacgctggcacccagttgatcggcgcgagatttaatcgccgcgacaatttgcgacggcgcgtgcagggccagactggaggtggcaacgccaatcagcaacgactgtttgcccgccagttgttgtgccacgcggttgggaatgtaattcagctccgccatcgccgcttccactttttcccgcgttttcgcagaaacgtggctggcctggttcaccacgcgggaaacggtctgataagagacaccggcatactctgcgacatcgtataacgttactggtttcacattcaccaccctgaattgactctcttccgggcgctatcatgccataccgcgaaaggttttgcgccattcgatggtgtcggatcgagatcgatctcgatcccgcgaaattaatacgactcactataggggaattgtgagcggataacaattcctactagagattaaagaggagaaactatatatgaaagcaattttcgtactgaaacatcttaatcatgctaaggaggttttctaatggtactgccgggcctcttgcgggatatcgtccattccgacagcatcgccagtcactatggcgtgctgctagcgctata…
encoding: naseq.html

https://synbiohub.org/public/SEGA/SEGA058_seq/1
type: Sequence
persistent identity: SEGA058 seq
display id: SEGA058_seq
version: 1
owned by: carbay
owned by: morno
elements: gtgcatggtctgcaggcgctgccgagccgtattgagcagatgctgtctcaggacaaacgcattgaagcgctggcagaagatttctctgacaaacatcacgcgctgttcctgggccgtggcgatcagtacccaatcgcgctggaaggcgcattgaagttgaaagagatctcttacattcacgctgaagcctacgctgctggcgaactgaaacacggtccgctggcgctaattgatgccgatatgccggttattgttgttgcaccgaacaacgaattgctggaaaaactgaaatccaacattgaagaagttcgcgcgcgtggcggtcagttgtatgtcttcgccgatcaggatgcgggttttgtaagtagcgataacatgcacatcatcgagatgccgcatgtggaagaggtgattgcaccgatcttctacaccgttccgctgcagctgctggcttaccatgtcgcgctgatcaaaggcaccgacgttgaccagccgcgtaacctggcaaaatcggttacggttgagtaataaatggatctcggtaccaaattccagaaaagagacgctttcgagcgtcttttttcgttttggtccgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgccagggtggtttttcttttcaccagtgagacgggcaacagctgattgcccttcaccgcctggccctgagagagttgcagcaagcggtccacgctggtttgccccagcaggcgaaaatcctgtttgatggtggttaacggcgggatataacatgagctgtcttcggtatcgtcgtatcccactaccgagatgtccgcaccaacgcgcagcccggactcggtaatggcgcgcattgcgcccagcgccatctgatcgttggcaaccagcatcgcagtgggaacgatgccctcattcagcatttgcatggtttgttgaaaaccggacatggcactccagtcgccttcccgttccgctatcggctgaatttgattgcgagtgagatatttatgccagccagccagacgcagacgcgccgagacagaacttaatgggcccgctaacagcgcgatttgctggtgacccaatgcgaccagatgctccacgcccagtcgcgtaccgtcttcatgggagaaaataatactgttgatgggtgtctggtcagagacatcaagaaataacgccggaacattagtgcaggcagcttccacagcaatggcatcctggtcatccagcggatagttaatgatcagcccactgacgcgttgcgcgagaagattgtgcaccgccgctttacaggcttcgacgccgcttcgttctaccatcgacaccaccacgctggcacccagttgatcggcgcgagatttaatcgccgcgacaatttgcgacggcgcgtgcagggccagactggaggtggcaacgccaatcagcaacgactgtttgcccgccagttgttgtgccacgcggttgggaatgtaattcagctccgccatcgccgcttccactttttcccgcgttttcgcagaaacgtggctggcctggttcaccacgcgggaaacggtctgataagagacaccggcatactctgcgacatcgtataacgttactggtttcacattcaccaccctgaattgactctcttccgggcgctatcatgccataccgcgaaaggttttgcgccattcgatggtgtcggatcgagatcgatctcgatcccgcgaaattaatacgactcactataggggaattgtgagcggataacaattcctactagagattaaagaggagaaactatatatgaaagcaattttcgtactgaaacatcttaatcatgctaaggaggttttctaatgagcaaaggagaagaacttttcactggagttgtgccaattcttgttgaattagatggtgatgttaatgggcacaaatt…
encoding: naseq.html

https://synbiohub.org/public/SEGA/SEGA051_seq/1
type: Sequence
persistent identity: SEGA051 seq
display id: SEGA051_seq
version: 1
owned by: carbay
owned by: morno
elements: gtgcatggtctgcaggcgctgccgagccgtattgagcagatgctgtctcaggacaaacgcattgaagcgctggcagaagatttctctgacaaacatcacgcgctgttcctgggccgtggcgatcagtacccaatcgcgctggaaggcgcattgaagttgaaagagatctcttacattcacgctgaagcctacgctgctggcgaactgaaacacggtccgctggcgctaattgatgccgatatgccggttattgttgttgcaccgaacaacgaattgctggaaaaactgaaatccaacattgaagaagttcgcgcgcgtggcggtcagttgtatgtcttcgccgatcaggatgcgggttttgtaagtagcgataacatgcacatcatcgagatgccgcatgtggaagaggtgattgcaccgatcttctacaccgttccgctgcagctgctggcttaccatgtcgcgctgatcaaaggcaccgacgttgaccagccgcgtaacctggcaaaatcggttacggttgagtaataaatggatctcggtaccaaattccagaaaagagacgctttcgagcgtcttttttcgttttggtccgcgttgcgcttaatctttctgcgaattgagatgacgccactggctgggcgtcatcccggtttcccgggtaaacaccaccgaaaaatagttactatcttcaaagccacattcggtcgaaatatcactgattaacaggcggctatgctggagaagatattgcgcatgacacactctgacctgtcgcagatattgattgatggtcattccagtctgctggcgaaattgctgacgcaaaacgcgctcactgcacgatgcctcatcacaaaatttatccagcgcaaagggacttttcaggctagccgccagccgggtaatcagcttatccagcaacgtttcgctggatgttggcggcaacgaatcactggtgtaacgatggcgattcagcaacatcaccaactgcccgaacagcaactcagccatttcgttagcaaacggcacatgctgactactttcatgctcaagctgaccaataacctgccgcgcctgcgccatccccatgctacctaagcgccagtgtggttgccctgcgctggcgttaaatcccggaatcgccccctgccagtcaagattcagcttcagacgctccgggcaataaataatattctgcaaaaccagatcgttaacggaagcgtaggagtgtttatcatcagcatgaatgtaaaagagatcgccacgggtaatgcgataagggcgatcgttgagtacatgcaggccattaccgcgccagacaatcaccagctcacaaaaatcatgtgtatgttcagcaaagacatcttgcggataacggtcagccacagcgactgcctgctggtcgctggcaaaaaaatcatctttgagaagttttaactgatgcgccaccgtggctacctcggccagagaacgaagttgattattcgcaatatggcgtacaaatacgttgagaagattcgcgttattgcagaaagccatcccgtccctggcgaatatcacgcggtgaccagttaaactctcggcgaaaaagcgtcgaaaagtggttactgtcgctgaatccacagcgataggcgatgtcagtaacgctggcctcgctgtggcgtagcagatgtcgggctttcatcagtcgcaggcggttcaggtatcgctgaggcgtcagtcccgtttgctgcttaagctgccgatgtagcgtacgcagtgaaagagaaaattgatccgccacggcatcccaattcacctcatcggcaaaatggtcctccagccaggccagaagcaagttgagacgtgatgcgctgttttccaggttctcctgcaaactgcttttacgcagcaagagcagtaattgcataaacaagatctcgcgactggcggtcgagggtaaatcattttccccttcctgctgttccatctgtgcaaccagctgtcgcacctgctgc…
encoding: naseq.html

https://synbiohub.org/public/SEGA/SEGA080_seq/1
type: Sequence
persistent identity: SEGA080 seq
display id: SEGA080_seq
version: 1
owned by: carbay
owned by: morno
elements: gtgcatggtctgcaggcgctgccgagccgtattgagcagatgctgtctcaggacaaacgcattgaagcgctggcagaagatttctctgacaaacatcacgcgctgttcctgggccgtggcgatcagtacccaatcgcgctggaaggcgcattgaagttgaaagagatctcttacattcacgctgaagcctacgctgctggcgaactgaaacacggtccgctggcgctaattgatgccgatatgccggttattgttgttgcaccgaacaacgaattgctggaaaaactgaaatccaacattgaagaagttcgcgcgcgtggcggtcagttgtatgtcttcgccgatcaggatgcgggttttgtaagtagcgataacatgcacatcatcgagatgccgcatgtggaagaggtgattgcaccgatcttctacaccgttccgctgcagctgctggcttaccatgtcgcgctgatcaaaggcaccgacgttgaccagccgcgtaacctggcaaaatcggttacggttgagtaataaatggatctcggtaccaaattccagaaaagagacgctttcgagcgtcttttttcgttttggtccaccgtattcatcagcactgtcctgctccttgtgatggtttacaaacgtaaagagtctctttaatacctgtttttgcttcatattgttcagcgacagcttgctgtacggcaggcaccagctcttccgggatcagcgcgacgatacagccgccaaatccgccgccggtcatgcgtacgccacctttgtcgccaatcacagctttgacgatttctaccagagtgtcaatttgcggcacggtgatttcgaaatcatcgcgcatagaggcatgagactccgccatcaactcgcccatacgtttcaggtcgccttgctccagcgcgctggcagcttcaacggtgcgggcgttttcagtcagtatatgacgcacgcgttttgccacgatcgggtccagttcatgcgcaacagcgttgaactcttcaatggtgacatcacgcagggctggctgctggaagaaacgcgcaccggtttcgcactgttcacgacgggtgttgtattcgctgccaaccagggtacgtttgaagttactgttgatgatgacgacagccacacctttgggcatggaaactgctttggtccccagtgagcggcaatcgatcagcaaggcatgatctttcttgccgagcgcggaaattagctgatccatgatcccgcagttacagcctacaaactggttttctgcttcctgaccgttaagcgcgatttgtgcgccgtccagcggcagatgataaagctgctgcaatacggttccgaccgcgacttccagtgaagcggaagaacttaacccggcaccctgcggcacattgccgctgatcaccatgtccacgccgccgaagctgttgttacgcagttgcagatgtttcaccacgccacgaacgtagttagcccattgatagttttcatgtgcgacaatgggcgcatcgagggaaaactcgtcgagctgattttcataatcggctgccatcacgcgaactttacggtcatcgcgtggtgcacaactgatcacggtttgataatcaatcgcgcagggcagaacgaaaccgtcgttgtagtcggtgtgttcaccaatcaaattcacgcggccaggcgcctgaatggtgtgagtggcagggtagccaaatgcgttggcaaacagagattgtgttttttctttcagactcatgattgcctcctgggttagttcctcaccttgtcgtattatactatgccgatatactatgccgatgattaattgtcaacctttaatgaattataaaacgaaaggctcagtcgaaagactgggcctttcgttttatgcgttgcgcttaatctttctgcgaattgagatgacgccactggctgggcgtcatcccggtttcccgggtaaacaccaccgaaaaatagttactatcttcaaagccacattcggt…
encoding: naseq.html