##gff-version 3
##sequence-region IBMc451 9 4612
IBMc451	.	promoter	1581	1634	.	+	0	ID=annotation17;Name=PlacIQ
IBMc451	.	primer_binding_site	3322	3341	.	-	0	ID=annotation7;Name=VR
IBMc451	.	ribosome_entry_site	2765	2772	.	+	0	ID=annotation2;Name=RBS for CDS 1
IBMc451	.	biological_region	2746	2830	.	+	0	ID=annotation8;Name=BCD1
IBMc451	.	primer_binding_site	1371	1390	.	+	0	ID=annotation1;Name=VF2
IBMc451	.	promoter	9	97	.	+	0	ID=annotation5;Name=tetR promoter
IBMc451	.	terminator	2530	2582	.	+	0	ID=annotation9;Name=L3S3P21
IBMc451	.	CDS	1663	2265	.	+	0	ID=annotation13;Name=PhlF
IBMc451	.	biological_region	3207	3227	.	+	0	ID=annotation12;Name=RFC10 Suffix
IBMc451	.	ribosome_entry_site	1635	1662	.	+	0	ID=annotation15;Name=phl2
IBMc451	.	CDS	98	1291	.	+	0	ID=annotation3;Name=tetR
IBMc451	.	origin_of_replication	3788	4612	.	+	0	ID=annotation10;Name=p15A origin of replication
IBMc451	.	promoter	2602	2667	.	+	0	ID=annotation6;Name=PPhlF
IBMc451	.	insulator	2668	2742	.	+	0	ID=annotation4;Name=RiboJ
IBMc451	.	biological_region	1494	1515	.	+	0	ID=annotation21;Name=RFC10 Prefix (full)
IBMc451	.	terminator	2275	2529	.	+	0	ID=annotation11;Name=IOT
IBMc451	.	CDS	2831	2956	.	+	0	ID=annotation20;Name=SYNZIP18
IBMc451	.	ribosome_entry_site	2816	2824	.	+	0	ID=annotation0;Name=RBS for CDS2
IBMc451	.	terminator	3154	3206	.	+	0	ID=annotation14;Name=ECK120033736
IBMc451	.	CDS	2972	3124	.	+	0	ID=annotation18;Name=mCherry*(186-236)
IBMc451	.	terminator	1516	1576	.	+	0	ID=annotation19;Name=L3S2P21
IBMc451	.	CDS	3125	3142	.	+	0	ID=annotation16;Name=H6
>IBMc451
tactagaggagattctcatgtttgacagcttatcatcgataagctttaatgcggtagtttatcacagttaaattgctaa
cgcagtcaggcaccgtgtatgaaatctaacaatgcgctcatcgtcattctcggcaccgtcaccctggacgctgtaggca
taggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagtattgccagtcactatggcgt
gctgcttgcgctctatgcgttgatgcaatttctttgcgcacccgttctcggagccctgtccgaccgctttggccgccgt
ccagtcctgctcgcttcgctccttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggattctct
acgccggacgcatcgtggcgggcatcacgggtgccacaggtgcggttgctggtgcctatatcgccgacatcaccgatgg
ggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccggggga
ctgttgggtgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctcctcctgggctgct
tccttatgcaggaatcgcataagggagagcgccgtccgatgcccttgcgtgccttcaatccagtcagctccttccggtg
ggcgcggggcatgactatcgtcgccgcacttatgactgttttctttatcatgcaactcgtaggacaggttccggcagcg
ctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatct
tgcacgccctcgctcaagccttcgtcacgggccccgccaccaaacgtttcggcgagaagcaggccattatcgcgggcat
ggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgcggctggatggccttccccattatgattcttctc
gcttccggcggcatcggtatgcccgcgttgcaggccatgctgtcccgccaagtagatgacgaccatcagggacagcttc
aagggtcgctcgcggctcttaccagcctcacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggc
gagcacatggaacgggttggcatggattgtaggtgccgccctttaccttgtctgcctccccgcgttgcgtcgcggtgca
tggagccgggccacctcgacctaataatactagctccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttct
ttaaaaccgaaaagattacttcgcgtttgccacctgacgtctaagaaaaggaatattcagcaatttgcccgtgccgaag
aaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaattagttagttagcccttagtgactcgaattcgc
ggccgcttctagagctcggtaccaaattccagaaaagaggcctcccgaaaggggggccttttttcgttttggtccaatg
gcggcgcgccatcgaatggtgcaaaacctttcgcggtatggcatgatagcgcccggaagagagtcaattcatgggggtg
aatatggcacgtaccccgagccgtagcagcattggtagcctgcgtagtccgcatacccataaagcaattctgaccagca
ccattgaaatcctgaaagaatgtggttatagcggtctgagcattgaaagcgtggcacgtcgcgccggtgcaggcaaacc
gaccatttatcgttggtggaccaacaaagcagcactgattgccgaagtgtatgaaaatgaaatcgaacaggtacgtaaa
tttccggatttgggtagctttaaagccgatctggattttctgctgcataatctgtggaaagtttggcgtgaaaccattt
gtggtgaagcatttcgttgtgttattgcagaagcacagttggaccctgtaaccctgacccaactgaaagatcagtttat
ggaacgtcgtcgtgagataccgaaaaaactggttgaagatgccattagcaatggtgaactgccgaaagatatcaatcgt
gaactgctgctggatatgatttttggtttttgttggtatcgcctgctgaccgaacagttgaccgttgaacaggatattg
aagaatttaccttcctgctgattaatggtgtttgtccgggtacacagtgttgataaggatcctaattggtaacgaatca
gacaattgacggctcgagggagtagcatagggtttgcagaatccctgcttcgtccatttgacaggcacattatgcatcg
atgataagctgtcaaacatgagcagatcctctacgccggacgcatcgtggccggcatcaccggcgccacaggtgcggtt
gctggcgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcaaatattttatct
gccaattattgaaggcctccctaacggggggcctttttttgtttctggtctcccgcttaacgatcgttggctgcgacgt
acggtggaatctgattcgttaccaattgacatgatacgaaacgtaccgtatcgttaaggtagctgtcaccggatgtgct
ttccggtctgatgagtccgtgaggacgaaacagcctctacaaataattttgtttaataaGGGCCCAAGTTCACTTAAAA
AGGAGATCAACAATGAAAGCAATTTTCGTACTGAAACATCTTAATCATGCACAGGAGACTTTCTAatgagcatcgcggc
gaccctggagaacgatctggcgcgtctggaaaacgaaaacgctcgtctcgaaaaagacatcgcgaacctggaacgtgac
ctggcgaaactggagcgtgaagaagcgtacttcggaggttcaggtggttacaaggccaagaagcccgtgcagctgcccg
gcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgc
cgagggccgccactccaccggcggcatggacgagctgtacaagcatcatcaccatcaccactaatactagagaacgcat
gagAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTtttattgcgctactagtagcggccgctgcaggagtcactaagg
gttagttagttagattagcagaaagtcaaaagcctccgaccggaggcttttgactaaaacttcccttggggttatcatt
ggggctcactcaaaggcggtaatcagataaaaaaaatccttagctttcgctaaggatgatttctgctagagatggaata
gactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaat
ctggagccggtgagcgtggatctcgcggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagttat
ctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaagcat
tggtaactgtcagaccaagtttactcatatatactttagattgatttaaaacttcatttttaatttaaaaggatctagg
tgaagatcctttttgataatctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccttaat
aagatgatcttcttgagatcgttttggtctgcgcgtaatctcttgctctgaaaacgaaaaaaccgccttgcagggcggt
ttttcgaaggttctctgagctaccaactctttgaaccgaggtaactggcttggaggagcgcagtcaccaaaacttgtcc
tttcagtttagccttaaccggcgcatgacttcaagactaactcctctaaatcaattaccagtggctgctgccagtggtg
cttttgcatgtctttccgggttggactcaagacgatagttaccggataaggcgcagcggtcggactgaacggggggttc
gtgcatacagtccagcttggagcgaactgcctacccggaactgagtgtcaggcgtggaatgagacaaacgcggccataa
cagcggaatgacaccggtaaaccgaaaggcaggaacaggagagcgcacgagggagccgccaggggaaacgcctggtatc
tttatagtcctgtcgggtttcgccaccactgatttgagcgtcagatttcgtgatgcttgtcaggggggcggagcctatg
gaaaaacggctttgccgcggccctctcacttccctgttaagtatcttcctggcatcttccaggaaatctccgccccgtt
cgtaagccatttccgctcgccgcagtcgaacgaccgagcgtagcgagtcagtgagcgaggaagcggaatatatcctgta
tcacatattctgctgacgcaccggtgcagccttttttctcctgccacatgaagcacttcactgacaccctcatcagtgc
caacatagtaagccagtatacactccgctagcgctgaggtctgcctcgtgaagaaggtgttgctgactcataccaggcc
tgaatcgccccatcatccagccagaaagtgagggagccacggttgatgagagctttgttgtaggtggaccagttggtga
ttttgaacttttgctttgccacggaacggtctgcgttgtcgggaagatgcgtgatctgatccttcaactcagcaaaagt
tcgatttattcaacaaagccacgttgtgtctcaaaatctctgatgttacattgcacaagataaaaatatatcatcatga
acaataaaactgtctgcttacataaacagtaatacaaggggtgtt