##gff-version 3
##sequence-region IBMc418 9 5502
IBMc418	.	primer_binding_site	4212	4231	.	-	0	ID=annotation19;Name=VR
IBMc418	.	ribosome_entry_site	3369	3381	.	+	0	ID=annotation8;Name=BBa_B0032
IBMc418	.	insulator	3286	3360	.	+	0	ID=annotation15;Name=RiboJ
IBMc418	.	primer_binding_site	1371	1390	.	+	0	ID=annotation17;Name=VF2
IBMc418	.	terminator	2134	2194	.	+	0	ID=annotation16;Name=L3S2P21
IBMc418	.	CDS	2281	2883	.	+	0	ID=annotation13;Name=PhlF
IBMc418	.	promoter	2036	2133	.	-	0	ID=annotation1;Name=cat promoter
IBMc418	.	terminator	4044	4096	.	+	0	ID=annotation5;Name=ECK120033736
IBMc418	.	promoter	2199	2252	.	+	0	ID=annotation10;Name=PlacIQ
IBMc418	.	CDS	3391	3552	.	+	0	ID=annotation0;Name=M86C
IBMc418	.	terminator	3148	3200	.	+	0	ID=annotation4;Name=L3S3P21
IBMc418	.	biological_region	4097	4117	.	+	0	ID=annotation7;Name=RFC10 Suffix
IBMc418	.	terminator	2893	3147	.	+	0	ID=annotation14;Name=IOT
IBMc418	.	promoter	9	97	.	+	0	ID=annotation2;Name=tetR promoter
IBMc418	.	CDS	3556	4032	.	+	0	ID=annotation12;Name=SrpR
IBMc418	.	ribosome_entry_site	2253	2280	.	+	0	ID=annotation20;Name=phl2
IBMc418	.	CDS	98	1291	.	+	0	ID=annotation18;Name=tetR
IBMc418	.	origin_of_replication	4678	5502	.	+	0	ID=annotation9;Name=p15A origin of replication
IBMc418	.	biological_region	1494	1494	.	+	0	ID=annotation11;Name=RFC10 Prefix (full)
IBMc418	.	CDS	1517	2035	.	-	0	ID=annotation6;Name=cat (BsmBI-)
IBMc418	.	promoter	3220	3285	.	+	0	ID=annotation3;Name=PPhlF
>IBMc418
tactagaggagattctcatgtttgacagcttatcatcgataagctttaatgcggtagtttatcacagttaaattgctaa
cgcagtcaggcaccgtgtatgaaatctaacaatgcgctcatcgtcattctcggcaccgtcaccctggacgctgtaggca
taggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagtattgccagtcactatggcgt
gctgcttgcgctctatgcgttgatgcaatttctttgcgcacccgttctcggagccctgtccgaccgctttggccgccgt
ccagtcctgctcgcttcgctccttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggattctct
acgccggacgcatcgtggcgggcatcacgggtgccacaggtgcggttgctggtgcctatatcgccgacatcaccgatgg
ggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccggggga
ctgttgggtgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctcctcctgggctgct
tccttatgcaggaatcgcataagggagagcgccgtccgatgcccttgcgtgccttcaatccagtcagctccttccggtg
ggcgcggggcatgactatcgtcgccgcacttatgactgttttctttatcatgcaactcgtaggacaggttccggcagcg
ctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatct
tgcacgccctcgctcaagccttcgtcacgggccccgccaccaaacgtttcggcgagaagcaggccattatcgcgggcat
ggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgcggctggatggccttccccattatgattcttctc
gcttccggcggcatcggtatgcccgcgttgcaggccatgctgtcccgccaagtagatgacgaccatcagggacagcttc
aagggtcgctcgcggctcttaccagcctcacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggc
gagcacatggaacgggttggcatggattgtaggtgccgccctttaccttgtctgcctccccgcgttgcgtcgcggtgca
tggagccgggccacctcgacctaataatactagctccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttct
ttaaaaccgaaaagattacttcgcgtttgccacctgacgtctaagaaaaggaatattcagcaatttgcccgtgccgaag
aaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaattagttagttagcccttagtgactcgaattcgc
ggccgcttctagagCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAA
CTCACCCAGGGATTGGCTGAAACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAAC
ACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTC
AGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGA
AATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGG
TCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATG
TTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCCT
GAAAATCTCGATAACTCAAAAAATACGCCCGGTAGTGATCTTATTTCATTATGGTGAAAGTTGGAACCTCTTACGTGCC
ctcggtaccaaattccagaaaagaggcctcccgaaaggggggccttttttcgttttggtccaatggcggcgcgccatcg
aatggtgcaaaacctttcgcggtatggcatgatagcgcccggaagagagtcaattcatgggggtgaatatggcacgtac
cccgagccgtagcagcattggtagcctgcgtagtccgcatacccataaagcaattctgaccagcaccattgaaatcctg
aaagaatgtggttatagcggtctgagcattgaaagcgtggcacgtcgcgccggtgcaggcaaaccgaccatttatcgtt
ggtggaccaacaaagcagcactgattgccgaagtgtatgaaaatgaaatcgaacaggtacgtaaatttccggatttggg
tagctttaaagccgatctggattttctgctgcataatctgtggaaagtttggcgtgaaaccatttgtggtgaagcattt
cgttgtgttattgcagaagcacagttggaccctgtaaccctgacccaactgaaagatcagtttatggaacgtcgtcgtg
agataccgaaaaaactggttgaagatgccattagcaatggtgaactgccgaaagatatcaatcgtgaactgctgctgga
tatgatttttggtttttgttggtatcgcctgctgaccgaacagttgaccgttgaacaggatattgaagaatttaccttc
ctgctgattaatggtgtttgtccgggtacacagtgttgataaggatcctaattggtaacgaatcagacaattgacggct
cgagggagtagcatagggtttgcagaatccctgcttcgtccatttgacaggcacattatgcatcgatgataagctgtca
aacatgagcagatcctctacgccggacgcatcgtggccggcatcaccggcgccacaggtgcggttgctggcgcctatat
cgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcaaatattttatctgccaattattgaag
gcctccctaacggggggcctttttttgtttctggtctcccgcttaacgatcgttggctgcgacgtacggtggaatctga
ttcgttaccaattgacatgatacgaaacgtaccgtatcgttaaggtagctgtcaccggatgtgctttccggtctgatga
gtccgtgaggacgaaacagcctctacaaataattttgtttaatactagagtcacacaggaaagtactagATGagctcct
ctttacaattggcaccagaaatagaaaagttgcctcagagtgatatttactgggaccccatcgtttctattacggagac
tggagtcgaagaggtttttgatttgactgtgccaggactacgtaactttgtcgccaatgacatcattgtacataacTCA
AAACTGGAAGTTCTGCAAGCAGTTCTGGCAAGCCGTCAGCATCCGCTGGAACTGGATTTTACACCGGATCTGGGTATTG
AACGTAGCTGGGAAGCAGTTGTTGTTGCAATGCTGGATGCAGTTCATAGTCCGCAGAGCAAACAGTTTAGCGAAATTCT
GATTTATCAGGGTCTGGATGAAAGCGGTCTGATTCATAATCGTATGGTTCAGGCAAGCGATCGTTTTCTGCAATATATT
CATCAGGTTCTGCGTCATGCAGTTACCCAGGGTGAACTGCCGATTAATCTGGATCTGCAAACCAGCATTGGTGTTTTTA
AAGGTCTGATTACCGGTCTGCTGTATGAAGGTCTGCGTAGCAAAGATCAGCAGGCACAGATTATCAAAGTTGCACTGGG
TAGCTTTTGGGCACTGCTGCGTGAACCGCCTCGTTTTCTGCTGTGTGAAGAAGCACAGATTAAACAGGTGAAATCCTTC
GAATAAtactagagaacgcatgagAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTtttattgcgctactagtagcgg
ccgctgcaggagtcactaagggttagttagttagattagcagaaagtcaaaagcctccgaccggaggcttttgactaaa
acttcccttggggttatcattggggctcactcaaaggcggtaatcagataaaaaaaatccttagctttcgctaaggatg
atttctgctagagatggaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctg
gctggtttattgctgataaatctggagccggtgagcgtggatctcgcggtatcattgcagcactggggccagatggtaa
gccctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagata
ggtgcctcactgattaagcattggtaactgtcagaccaagtttactcatatatactttagattgatttaaaacttcatt
tttaatttaaaaggatctaggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgagttttcgttcca
ctgagcgtcagaccccttaataagatgatcttcttgagatcgttttggtctgcgcgtaatctcttgctctgaaaacgaa
aaaaccgccttgcagggcggtttttcgaaggttctctgagctaccaactctttgaaccgaggtaactggcttggaggag
cgcagtcaccaaaacttgtcctttcagtttagccttaaccggcgcatgacttcaagactaactcctctaaatcaattac
cagtggctgctgccagtggtgcttttgcatgtctttccgggttggactcaagacgatagttaccggataaggcgcagcg
gtcggactgaacggggggttcgtgcatacagtccagcttggagcgaactgcctacccggaactgagtgtcaggcgtgga
atgagacaaacgcggccataacagcggaatgacaccggtaaaccgaaaggcaggaacaggagagcgcacgagggagccg
ccaggggaaacgcctggtatctttatagtcctgtcgggtttcgccaccactgatttgagcgtcagatttcgtgatgctt
gtcaggggggcggagcctatggaaaaacggctttgccgcggccctctcacttccctgttaagtatcttcctggcatctt
ccaggaaatctccgccccgttcgtaagccatttccgctcgccgcagtcgaacgaccgagcgtagcgagtcagtgagcga
ggaagcggaatatatcctgtatcacatattctgctgacgcaccggtgcagccttttttctcctgccacatgaagcactt
cactgacaccctcatcagtgccaacatagtaagccagtatacactccgctagcgctgaggtctgcctcgtgaagaaggt
gttgctgactcataccaggcctgaatcgccccatcatccagccagaaagtgagggagccacggttgatgagagctttgt
tgtaggtggaccagttggtgattttgaacttttgctttgccacggaacggtctgcgttgtcgggaagatgcgtgatctg
atccttcaactcagcaaaagttcgatttattcaacaaagccacgttgtgtctcaaaatctctgatgttacattgcacaa
gataaaaatatatcatcatgaacaataaaactgtctgcttacataaacagtaatacaaggggtgtt