##gff-version 3 ##sequence-region IBMc394 56 5740 IBMc394 . terminator 2184 2236 . + 0 ID=annotation12;Name=ECK120033736 IBMc394 . biological_region 56 77 . + 0 ID=annotation9;Name=RFC10 Prefix (full) IBMc394 . CDS 1300 1317 . + 0 ID=annotation1;Name=H6 IBMc394 . promoter 2459 2556 . + 0 ID=annotation4;Name=cat promoter IBMc394 . promoter 984 1267 . + 0 ID=annotation15;Name=PBAD(SapI-) IBMc394 . CDS 78 956 . - 0 ID=annotation5;Name=araC IBMc394 . CDS 1852 1893 . + 0 ID=annotation3;Name=Long Linker IBMc394 . biological_region 2237 2257 . + 0 ID=annotation7;Name=RFC10 Suffix IBMc394 . terminator 2312 2406 . + 0 ID=annotation0;Name=lambda t0 terminator IBMc394 . ribosome_entry_site 1276 1290 . + 0 ID=annotation11;Name=BBa_B0030 IBMc394 . primer_binding_site 5723 5740 . + 0 ID=annotation8;Name=pSEVA PS1 Fwd IBMc394 . CDS 1318 1851 . + 0 ID=annotation13;Name=29-1 PACE T7 RNAP 1-179 variant: L32S, E35G, A83A, K98R, L106L, Q107K, T122S, A144T IBMc394 . primer_binding_site 2366 2382 . - 0 ID=annotation10;Name=pSEVA PS2 Rev IBMc394 . CDS 1894 2172 . + 0 ID=annotation2;Name=FRB E coli optimized IBMc394 . CDS 3534 4682 . - 0 ID=annotation14;Name=trfA CDS (replication protein) IBMc394 . CDS 2557 3216 . + 0 ID=annotation6;Name=cat >IBMc394 ttaattaaagcggataacaatttcacacaggaggccgcctaggccgcggccgcgcgaattcgcggccgcttctagagtt atgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattt tttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtg ctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaa gatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatc gctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgc agtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcc caaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagtt aagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccg tagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcacca ccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataacc gttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgct tcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcg tcttttactggcActtctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagc catgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactt tgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctc cattactagagattaaagaggagaaaTACTAGATGcatcatcaccatcaccacaacacgattaacatcgctaagaacga cttctctgacatcgaactggctgctatcccgttcaacactctggctgaccattacggtgagcgtTCAgctcgcGGAcag ttggcccttgagcatgagtcttacgagatgggtgaagcacgcttccgcaagatgtttgagcgtcaacttaaagctggtg aggttgcggataacgctgccgccaagcctctcatcactaccctactccctaagatgattGCCcgcatcaacgactggtt tgaggaagtgaaagctaagcgcggcAGGcgcccgacagccttccagttcCTAAAAgaaatcaagccggaagccgtagcg tacatcaccattaagaccTCTctggcttgcctaaccagtgctgacaatacaaccgttcaggctgtagcaagcgcaatcg gtcggACCattgaggacgaggctcgcttcggtcgtatccgtgaccttgaagctaagcacttcaagaaaaacgttgagga acaactcaacaagcgcgtagggcacgtctacaagGGTGGCTCTGGCTCGAGCGGTGGCTCTGGCTCTGGCTCGAGCGCC GTGTTATGGCACGAACAGTGGTATGAGGGTTTGGATGACGCATCGCGCCAGTTCTTCGGAGAACATAACACCGAAAAAA TGTTTGCGGCACTGGAACCTCTTTATGAGATGCTTAAACGTGGGCCTGAAACGTTGCGTGAGATTTCTTTTCAGAACTC ATTTGGGCGTGATTTGAACGATGCCTACGAATGGCTGATGAATTACAAAAAGTCGAAAGATGTATCTAACCTGAATCAA GCATGGGATATTTACTATAATGTCTTTCGTAAAATCGGCtaatactagagaacgcatgagAAAGCCCCCGGAAGATCAC CTTCCGGGGGCTTTtttattgcgctactagtagcggccgctgcaggcatgcaagcttgcggccgcgtcgtgactgggaa aaccctggcgactagtcttggactcctgttgatagatccagtaatgacctcagaactccatctggatttgttcagaacg ctcggttgccgccgggcgttttttattggtgagaatccaggggtccccaataattacgatttaaattggcgaaaatgag acgttgatcggcacgtaagaggttccaactttcaccataatgaaataagatcactaccgggcgtattttttgagttatc gagattttcaggagctaaggaagctaaaatggagaaaaaaatcactggatataccaccgttgatatatcccaatggcat cgtaaagaacattttgaggcatttcagtcagttgctcaatgtacctataaccagaccgttcagctggatattacggcct ttttaaagaccgtaaagaaaaataagcacaagttttatccggcctttattcacattcttgcccgcctgatgaatgctca tccggaatttcgtatggcaatgaaagacggtgagctggtgatatgggatagtgttcacccttgttacaccgttttccat gagcaaactgaaacgttttcatcgctctggagtgaataccacgacgatttccggcagtttctacacatatattcgcaag atgtggcgtgttacggtgaaaacctggcctatttccctaaagggtttattgagaatatgtttttcgtctcagccaatcc ctgggtgagtttcaccagttttgatttaaacgtggccaatatggacaacttcttcgcccccgttttcaccatgggcaaa tattatacgcaaggcgacaaggtgctgatgccgctggcgattcaggttcatcatgccgtttgtgatggcttccatgtcg gcagaatgcttaatgaattacaacagtactgcgatgagtggcagggcggggcgtaatttgacttttgtccttttccgct gcataaccctgcttcggggtcattatagcgattttttcggtatatccatcctttttcgcacgatatacaggattttgcc aaagggttcgtgtagactttccttggtgtatccaacggcgtcagccgggcaggataggtgaagtaggcccacccgcgag cgggtgttccttcttcactgtcccttattcgcacctggcggtgctcaacgggaatcctgctctgcgaggctggccgtag gccggccgcgatgcaggtggctgctgaacccccagccggaactgaccccacaaggccctagcgtttgcaatgcaccagg tcatcattgacccaggcgtgttccaccaggccgctgcctcgcaactcttcgcaggcttcgccgacctgctcgcgccact tcttcacgcgggtggaatccgatccgcacatgaggcggaaggtttccagcttgagcgggtacggctcccggtgcgagct gaaatagtcgaacatccgtcgggccgtcggcgacagcttgcggtacttctcccatatgaatttcgtgtagtggtcgcca gcaaacagcacgacgatttcctcgtcgatcaggacctggcaacgggacgttttcttgccacggtccaggacgcggaagc ggtgcagcagcgacaccgattccaggtgcccaacgcggtcggacgtgaagcccatcgccgtcgcctgtaggcgcgacag gcattcctcggccttcgtgtaataccggccattgatcgaccagcccaggtcctggcaaagctcgtagaacgtgaaggtg atcggctcgccgataggggtgcgcttcgcgtactccaacacctgctgccacaccagttcgtcatcgtcggcccgcagct cgacgccggtgtaggtgatcttcacgtccttgttgacgtggaaaatgaccttgttttgcagcgcctcgcgcgggatttt cttgttgcgcgtggtgaacagggcagagcgggccgtgtcgtttggcatcgctcgcatcgtgtccggccacggcgcaata tcgaacaaggaaagctgcatttccttgatctgctgcttcgtgtgtttcagcaacgcggcctgcttggcttcgctgacct gttttgccaggtcctcgccggcggtttttcgcttcttggtcgtcatagttcctcgcgtgtcgatggtcatcgacttcgc caaacctgccgcctcctgttcgagacgacgcgaacgctccacggcggccgatggcgcgggcagggcagggggagccagt tgcacgctgtcgcgctcgatcttggccgtagcttgctggactatcgagccgacggactggaaggtttcgcggggcgcac gcatgacggtgcggcttgcgatggtttcggcatcctcggcggaaaaccccgcgtcgatcagttcttgcctgtatgcctt ccggtcaaacgtccgattcattcaccctccttgcgggattgccccggaattaattccccggatcgatccgtcgatcttg atcccctgcgccatcagatccttggcggcaagaaagccatccagtttactttgcagggcttcccaaccttaccagaggg cgccccagctggcaattccggttcgcttgctgtccataaaaccgcccagtctagctatcgccatgtaagcccactgcaa gctacctgctttctctttgcgcttgcgttttcccttgtccagatagcccagtagctgacattcatccggggtcagcacc gtttctgcggactggctttctacgtggctgccatttttggggtgaggccgttcgcggccgaggggcgcagcccctgggg ggatgggaggcccgcgttagcgggccgggagggttcgagaagggggggcaccccccttcggcgtgcgcggtcacgcgca cagggcgcagccctggttaaaaacaaggtttataaatattggtttaaaagcaggttaaaagacaggttagcggtggccg aaaaacgggcggaaacccttgcaaatgctggattttctgcctgtggacagcccctcaaatgtcaataggtgcgcccctc atctgtcagcactctgcccctcaagtgtcaaggatcgcgcccctcatctgtcagtagtcgcgcccctcaagtgtcaata ccgcagggcacttatccccaggcttgtccacatcatctgtgggaaactcgcgtaaaatcaggcgttttcgccgatttgc gaggctggccagctccacgtcgccggccgaaatcgagcctgcccctcatctgtcaacgccgcgccgggtgagtcggccc ctcaagtgtcaacgtccgcccctcatctgtcagtgagggccaagttttccgcgaggtatccacaacgccggcggcccta catggctctgctgtagtgagtgggttgcgctccggcagcggtcctgatcccccgcagaaaaaaaggatctcaagaagat cctttgatcttttctacggcgcgcccagctgtctagggcggcggatttgtcctactcaggagagcgttcaccgacaaac aacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcct