[ { "collection": "measurement_std", "signal_carrier_units": "RNAP/s", "normalization_instructions": "The values are converted from RPUG (from the Eco2C1G3T1 UCF) to RNAP/s with the following conversion factor: RPU = 0.019 RNAP/s (see 10.15252/msb.20209584). Note: change unit conversion factors accordingly if using a different or modified UCF." }, { "collection": "output_devices", "name": "BFP_reporter", "model": "BFP_reporter_model", "structure": "BFP_reporter_structure" }, { "collection": "models", "name": "BFP_reporter_model", "functions": { "response_function": "linear_response", "input_composition": "linear_input_composition" }, "parameters": [ { "name": "unit_conversion", "value": 0.019, "description": "Unit conversion" } ] }, { "collection": "structures", "name": "BFP_reporter_structure", "inputs": [ { "name": "in1", "part_type": "promoter" }, { "name": "in2", "part_type": "promoter" } ], "devices": [ { "name": "BFP_reporter_a", "components": [ "#in1", "BFP_reporter_cassette" ] }, { "name": "BFP_reporter_b", "components": [ "#in2", "BFP_reporter_cassette" ] } ] }, { "collection": "parts", "name": "BFP_reporter_cassette", "type": "cassette", "dnasequence": "GCTGTCACCGGATGTGCTTTCCGGTCTGATGAGTCCGTGAGGACGAAACAGCCTCTACAAATAATTTTGTTTAATACTAGAGaaagaggagaaaTACTAGATGGTGTCTAAGGGCGAAGAGCTGATTAAGGAGAACATGCACATGAAGCTGTACATGGAGGGCACCGTGGACAACCATCACTTCAAGTGCACATCCGAGGGCGAAGGCAAGCCCTACGAGGGCACCCAGACCATGCGCATCAAGGTGGTCGAGGGCGGCCCTCTCCCCTTCGCCTTCGACATCCTGGCTACTAGCTTCCTCTACGGCAGCAAGACCTTCATCAACCACACCCAGGGCATCCCCGACTTCTTCAAGCAGTCCTTCCCTGAGGGCTTCACATGGGAGCGCGTCACCACATACGAGGACGGGGGCGTGCTGACCGCTACCCAGGACACCAGCCTCCAGGACGGCTGCCTCATCTACAACGTCAAGATCCGGGGGGTGAACTTCACATCCAACGGCCCTGTGATGCAGAAGAAAACACTCGGCTGGGAGGCCTTCACCGAAACCCTGTACCCCGCTGACGGCGGCCTGGAAGGCCGTAACGACATGGCCCTGAAGCTCGTGGGCGGGAGCCATCTGATCGCAAACGCCAAGACCACATATCGTTCCAAGAAACCCGCTAAGAACCTCAAGATGCCTGGCGTCTACTATGTGGACTACCGCCTGGAACGTATCAAGGAGGCCAACAACGAAACGTACGTCGAGCAGCACGAGGTGGCAGTGGCCCGGTACTGCGACCTCCCTAGCAAACTGGGGCACAAGCTTAATTAAAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGG" }, { "collection": "output_devices", "name": "RFP_reporter", "model": "RFP_reporter_model", "structure": "RFP_reporter_structure" }, { "collection": "models", "name": "RFP_reporter_model", "functions": { "response_function": "linear_response", "input_composition": "linear_input_composition" }, "parameters": [ { "name": "unit_conversion", "value": 0.019, "description": "Unit conversion" } ] }, { "collection": "structures", "name": "RFP_reporter_structure", "inputs": [ { "name": "in1", "part_type": "promoter" }, { "name": "in2", "part_type": "promoter" } ], "devices": [ { "name": "RFP_reporter_a", "components": [ "#in1", "RFP_reporter_cassette" ] }, { "name": "RFP_reporter_b", "components": [ "#in2", "RFP_reporter_cassette" ] } ] }, { "collection": "parts", "name": "RFP_reporter_cassette", "type": "cassette", "dnasequence": "agaagtcaattaatgtgcttttaattctgatgagtcggtgacgacgaaacttcctctacaaataattttgtttaaTACTAGAGAAAGAGGGGAAATACTAGATGGCTTCCTCCGAAGACGTTATCAAAGAGTTCATGCGTTTCAAAGTTCGTATGGAAGGTTCCGTTAACGGTCACGAGTTCGAAATCGAAGGTGAAGGTGAAGGTCGTCCGTACGAAGGTACCCAGACCGCTAAACTGAAAGTTACCAAAGGTGGTCCGCTGCCGTTCGCTTGGGACATCCTGTCCCCGCAGTTCCAGTACGGTTCCAAAGCTTACGTTAAACACCCGGCTGACATCCCGGACTACCTGAAACTGTCCTTCCCGGAAGGTTTCAAATGGGAACGTGTTATGAACTTCGAAGACGGTGGTGTTGTTACCGTTACCCAGGACTCCTCCCTGCAAGACGGTGAGTTCATCTACAAAGTTAAACTGCGTGGTACCAACTTCCCGTCCGACGGTCCGGTTATGCAGAAAAAAACCATGGGTTGGGAAGCTTCCACCGAACGTATGTACCCGGAAGACGGTGCTCTGAAAGGTGAAATCAAAATGCGTCTGAAACTGAAAGACGGTGGTCACTACGACGCTGAAGTTAAAACCACCTACATGGCTAAAAAACCGGTTCAGCTGCCGGGTGCTTACAAAACCGACATCAAACTGGACATCACCTCCCACAACGAAGACTACACCATCGTTGAACAGTACGAACGTGCTGAAGGTCGTCACTCCACCGGTGCTTAAGATGTGGGGTCCGATCTAACTAAAAAGGCCgctCTGCGGCCTTTTTTCTTTTCACT" }, { "collection": "output_devices", "name": "YFP_reporter", "model": "YFP_reporter_model", "structure": "YFP_reporter_structure" }, { "collection": "models", "name": "YFP_reporter_model", "functions": { "response_function": "linear_response", "input_composition": "linear_input_composition" }, "parameters": [ { "name": "unit_conversion", "value": 0.019, "description": "Unit conversion" } ] }, { "collection": "structures", "name": "YFP_reporter_structure", "inputs": [ { "name": "in1", "part_type": "promoter" }, { "name": "in2", "part_type": "promoter" } ], "devices": [ { "name": "YFP_reporter_a", "components": [ "#in1", "YFP_reporter_cassette" ] }, { "name": "YFP_reporter_b", "components": [ "#in2", "YFP_reporter_cassette" ] } ] }, { "collection": "parts", "name": "YFP_reporter_cassette", "type": "cassette", "dnasequence": "AGCTGTCACCGGATGTGCTTTCCGGTCTGATGAGTCCGTGAGGACGAAACAGCCTCTACAAATAATTTTGTTTAATACTAGAGAAAGAGGAGAAATACTAGATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACAGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCTTCGGCTACGGCCTGCAATGCTTCGCCCGCTACCCCGACCACATGAAGCTGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCAATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTTAGCTACCAGTCCGCCCTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTGTACAAGTAAGCTCATGTATGTGTCTACGCGAGATTCTCGCCCGAGAACTTCTGCAAGGCACTGCTCTTGGCT" }, { "collection": "functions", "name": "linear_response", "equation": "c * x", "variables": [ { "name": "x", "map": "#//model/functions/input_composition" } ], "parameters": [ { "name": "c", "map": "#//model/parameters/unit_conversion" } ] }, { "collection": "output_devices", "name": "OC6_out", "model": "OC6_out_model", "structure": "OC6_out_structure", "description": "comms_device" }, { "collection": "models", "name": "OC6_out_model", "functions": { "response_function": "Hill_response", "input_composition": "linear_input_composition" }, "parameters": [ { "name": "ymax", "value": 0.1425, "description": "Maximal transcription (7.5 RPU * 0.019 for conversion to RNAP/s)" }, { "name": "ymin", "value": 0.00114, "description": "Minimal transcription (0.06 RPU * 0.019 for conversion to RNAP/s)" }, { "name": "kd", "value": 0.1, "description": "Dissociation equilibrium constant" }, { "name": "n", "value": 1.9, "description": "Cooperativety" }, { "name": "unit_conversion", "value": 0.102, "description": "Unit conversion" } ] }, { "collection": "structures", "name": "OC6_out_structure", "inputs": [ { "name": "in1", "part_type": "promoter" }, { "name": "in2", "part_type": "promoter" } ], "devices": [ { "name": "OC6_out_a", "components": [ "#in1", "#in2", "sLux_cassette" ] } ] }, { "collection": "parts", "type": "cassette", "name": "sLux_cassette", "dnasequence": "agcgctcaacgggtgtgcttcccgttctgatgagtccgtgaggacgaaagcgcctctacaaataattttgtttaacggttccaaagccagatactaaggaggtcccAcATGactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagccttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaattaatagCTTATTCCATAACAAAGCCGGGTAATTCCCGGCTTTGTTGTATCTGAACAATAAATGGATGCCCTGCGTAAGCGGGGCATTTTTCTTCCT" }, { "collection": "parts", "type": "cds", "name": "LuxI", "dnasequence": "ATGACTATAATGATAAAAAAATCGGATTTTTTGGCAATTCCATCGGAGGAGTATAAAGGTATTCTAAGCCTTCGTTATCAAGTGTTTAAGCAAAGACTTGAGTGGGACTTAGTTGTAGAAAATAACCTTGAATCAGATGAGTATGATAACTCAAATGCAGAATATATTTATGCTTGTGATGATACTGAAAATGTAAGTGGATGCTGGCGTTTATTACCTACAACAGGTGATTATATGCTGAAAAGTGTTTTTCCTGAATTGCTTGGTCAACAGAGTGCTCCCAAAGATCCTAATATAGTCGAATTAAGTCGTTTTGCTGTAGGTAAAAATAGCTCAAAGATAAATAACTCTGCTAGTGAAATTACAATGAAACTATTTGAAGCTATATATAAACACGCTGTTAGTCAAGGTATTACAGAATATGTAACAGTAACATCAACAGCAATAGAGCGATTTTTAAAGCGTATTAAAGTTCCTTGTCATCGTATTGGAGACAAAGAAATTCATGTATTAGGTGATACTAAATCGGTTGTATTGTCTATGCCTATTAATGAACAGTTTAAAAAAGCAGTCTTAAATTAA" }, { "collection": "output_devices", "name": "OHC12_out", "model": "OHC12_out_model", "structure": "OHC12_out_structure", "description": "comms_device" }, { "collection": "models", "name": "OHC12_out_model", "functions": { "response_function": "Hill_response", "input_composition": "linear_input_composition" }, "parameters": [ { "name": "ymax", "value": 0.0646, "description": "Maximal transcription (3.4 RPU * 0.019 for conversion to RNAP/s)" }, { "name": "ymin", "value": 0.00019, "description": "Minimal transcription (0.01 RPU * 0.019 for conversion to RNAP/s)" }, { "name": "kd", "value": 0.3, "description": "Dissociation equilibrium constant" }, { "name": "n", "value": 3.9, "description": "Cooperativety" }, { "name": "unit_conversion", "value": 0.102, "description": "Unit conversion" } ] }, { "collection": "structures", "name": "OHC12_out_structure", "inputs": [ { "name": "in1", "part_type": "promoter" }, { "name": "in2", "part_type": "promoter" } ], "devices": [ { "name": "OHC12_out_a", "components": [ "#in1", "#in2", "sCin_cassette" ] } ] }, { "collection": "parts", "type": "cassette", "name": "sCin_cassette", "dnasequence": "gctgtcaccggatgtgctttccggtctgatgagtccgtgaggacgaaacagcctctacaaataattttgtttaatggttccaaagccagatactaaggaggtcccAATGttcgttatcatccaggctcacgaataccagaaatacgctgctgttctggaccagatgttccgtctgcgtaaaaaagttttcgctgacaccctgtgctgggacgttccggttatcggtccgtacgaacgtgactcctacgactccctggctccggcttacctggtttggtgcaacgactcccgtacccgtctgtacggtggtatgcgtctgatgccgaccaccggtccgaccctgctgtacgacgttttccgtgaaaccttcccggacgctgctgacctgatcgctccgggtatctgggaaggtacccgtatgtgcatcgacgaagaagctatcgctaaagacttcccggaaatcgacgctggtcgtgctttctccatgatgctgctggctctgtgcgaatgcgctctggaccacggtatccacaccatgatctccaactacgaaccgtacctgaaacgtgtttacaaacgtgctggtgctgaagttgaagaactgggtcgtgctgacggttacggtaaatacccggtttgctgcggtgctttcgaagtttccgaccgtgttctgcgtaaaatgcgtgctgctctgggtctgaccctgccgctgtacgttcgtcacgttccggctcgttccgttgttacccagttcctggaaatggctgcttaatagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata" }, { "collection": "parts", "type": "cds", "name": "cinI", "dnasequence": "ATGTTCGTTATCATCCAGGCTCACGAATACCAGAAATACGCTGCTGTTCTGGACCAGATGTTCCGTCTGCGTAAAAAAGTTTTCGCTGACACCCTGTGCTGGGACGTTCCGGTTATCGGTCCGTACGAACGTGACTCCTACGACTCCCTGGCTCCGGCTTACCTGGTTTGGTGCAACGACTCCCGTACCCGTCTGTACGGTGGTATGCGTCTGATGCCGACCACCGGTCCGACCCTGCTGTACGACGTTTTCCGTGAAACCTTCCCGGACGCTGCTGACCTGATCGCTCCGGGTATCTGGGAAGGTACCCGTATGTGCATCGACGAAGAAGCTATCGCTAAAGACTTCCCGGAAATCGACGCTGGTCGTGCTTTCTCCATGATGCTGCTGGCTCTGTGCGAATGCGCTCTGGACCACGGTATCCACACCATGATCTCCAACTACGAACCGTACCTGAAACGTGTTTACAAACGTGCTGGTGCTGAAGTTGAAGAACTGGGTCGTGCTGACGGTTACGGTAAATACCCGGTTTGCTGCGGTGCTTTCGAAGTTTCCGACCGTGTTCTGCGTAAAATGCGTGCTGCTCTGGGTCTGACCCTGCCGCTGTACGTTCGTCACGTTCCGGCTCGTTCCGTTGTTACCCAGTTCCTGGAAATGGCTGCTTAA" }, { "collection": "output_devices", "name": "pC_HSL_out", "model": "pC_HSL_out_model", "structure": "pC_HSL_out_structure", "description": "comms_device" }, { "collection": "models", "name": "pC_HSL_out_model", "functions": { "response_function": "Hill_response", "input_composition": "linear_input_composition" }, "parameters": [ { "name": "ymax", "value": 0.114, "description": "Maximal transcription (6 RPU * 0.019 for conversion to RNAP/s)" }, { "name": "ymin", "value": 0.00038, "description": "Minimal transcription (0.02 RPU * 0.019 for conversion to RNAP/s)" }, { "name": "kd", "value": 0.2, "description": "Dissociation equilibrium constant" }, { "name": "n", "value": 2.0, "description": "Cooperativety" }, { "name": "unit_conversion", "value": 0.102, "description": "Unit conversion" } ] }, { "collection": "structures", "name": "pC_HSL_out_structure", "inputs": [ { "name": "in1", "part_type": "promoter" }, { "name": "in2", "part_type": "promoter" } ], "devices": [ { "name": "pC_HSL_out_a", "components": [ "#in1", "#in2", "sRpa_cassette" ] } ] }, { "collection": "parts", "type": "cassette", "name": "sRpa_cassette", "dnasequence": "AGTGGTCGTGATCTGAAACTCGATCACCTGATGAGCTCAAGGCAGAGCGAAACCACCTCTACAAATAATTTTGTTTAAtggttccaaagccagatactaaggaggtcccAATGcaggttcatgttattcgtcgtgaaaatcgtgcactgtatgcaggtctgctggaaaaatactttcgtattcgccatcagatctatgttgttgaacgtggttggaaagaactggatcgtccggatggtcgtgaaattgatcagtttgataccgaagatgcagtttatctgctgggtgttgataacgatgatattgttgcaggtatgcgtatggttccgaccaccagtccgaccctgctgagtgatgtttttccgcagctggcactggcaggtccggttcgtcgtcctgatgcatatgaactgagccgtatttttgttgttccgcgtaaacgtggtgaacatggtggtccgcgtgcagaagcagttattcaggcagcagcaatggaatatggtctgagcattggtctgagtgcatttaccattgttctggaaacctggtggctgcctcgtctggttgatcagggctggaaagcaaaaccgctgggtctgccgcaggatattaatggttttagcaccaccgcagttattgtggatgttgatgatgatgcatgggtgggtatttgtaatcgtcgtagcgttccgggtccgacactggaatggcgtggtctggaagcaattcgtcgtcatagcctgccggaatttcaggttattagcTAAAGTCGCGGCAGCAGCACAAGAAGATGGAGAAGGACACGAAACAAGTCGACATCATTTTTCGTTCGAAGCTCCCCGACATTTACATCCCCAACCATCTTCCGCTTCACTCGTACTGTTTTGAAAACATTTCCGAATTCAGCAGCCGGCCCTGTCTCATTAACGGCGCAAATAAGCAGATTTACACCTACGCGGATGTTGAACTTAATTCGCGCAAAGTCGCCGCCGGCCTCCATAAGCAGGGTATCCAGCCCAAAGATACGATCATGATCCTGCTTCCCAACTCGCCCGAATTCGTTTTCGCGTTTATTGGCGCGTCGTACCTGGGCGCAATCAGCACGATGGCCAACCCCCTTTTTACCCCCGCCGAGGTGGTTAAGCAAGCCAAGGCGAGCTCCGCCAAGATTATTGTCACGCAGGCATGCCACGTCAATAAAGTTAAGGATTACGCATTTGAAAATGATGTGAAGATTATCTGCATCGACAGCGCCCCCGAGGGCTGCCTGCACTTCTCGGTCCTTACGCAGGCCAATGAGCACGACATTCCCGAGGTGGAGATTCAGCCCGATGACGTCGTCGCGCTCCCCTATAGCAGCGGCACCACGGGTCTGCCCAAAGGTGTCATGCTCACCCACAAAGGCCTCGTGACCAGCGTGGCACAACAGGTGGACGGCGAAAACCCCAACCTTTACATCCATTCCGAGGACGTTATGCTGTGTGTGCTCCCCCTGTTTCACATTTACTCCCTTAACTCGGTCCTTCTTTGCGGCCTCCGTGTTGGCGCAGCAATCCTTATTATGCAAAAGTTCGATATTGTCAGCTTCCTGGAGCTCATTCAACGCTACAAAGTCACGATTGGCCCCTTCGTCCCCCCGATTGTGCTCGCGATTGCGAAGTCCCCCATGGTGGATGACTACGATCTCAGCAGCGTCCGTACGGTCATGTCCGGCGCGGCACCGCTCGGTAAAGAACTGGAGGATACGGTCCGGGCCAAATTCCCGAATGCGAAGCTGGGTCAGGGTTACGGCATGACCGAGGCAGGTCCCGTGCTCGCCATGTGCCTGGCATTCGCGAAGGAGCCCTTCGAGATCAAGAGCGGTGCGTGCGGCACGGTCGTTCGGAACGCGGAAATGAAAATCGTGGACCCCAAAACCGGCAATTCCCTTCCCCGCAACCAATCCGGCGAAATTTGCATCCGGGGTGACCAGATTATGAAGGGCTATCTTAATGACCCGGAAGCCACGGCGCGGACGATTGACAAAGAAGGTTGGCTGTATACCGGTGACATTGGTTACATCGACGATGACGACGAGCTTTTTATTGTGGACCGGCTCAAAGAACTCATCAAATATAAAGGCTTTCAAGTTGCGCCCGCGGAGCTGGAGGCACTGCTTCTTAACCATCCCAACATCTCGGATGCCGCGGTCGTGCCGATGAAGGATGAACAAGCGGGTGAAGTGCCGGTCGCGTTTGTGGTTCGGAGCAACGGCTCCACGATTACGGAGGATGAGGTTAAGGATTTTATTTCCAAGCAGGTTATCTTTTATAAACGGATTAAACGCGTTTTCTTCGTCGATGCCATCCCCAAATCCCCCAGCGGCAAGATCCTTCGGAAAGATCTTCGTGCGAAACTGGCAGCAGGcCTTCCGAATTAAAAGAGGAGAAAGGTACCATGCTGGCCATGAGCCCGCCGAAACCGGCAGTGGAACTGGATCGTCATATTGATCTGGACCAGGCACACGCTGTTGCAAGCGGCGGTGCACGTATCGTCCTGGCACCTCCGGCCCGTGATCGCTGCCGTGCATCCGAAGCTCGTCTGGGTGCAGTCATTCGTGAAGCTCGCCATGTGTATGGCCTGACCACGGGTTTTGGTCCGCTGGCAAACCGTCTGATCAGCGGTGAAAATGTGCGCACCCTGCAAGCTAACCTGGTTCATCACCTGGCCAGCGGCGTGGGTCCGGTTCTGGATTGGACCACGGCACGTGCAATGGTCCTGGCACGCCTGGTGAGCATTGCACAAGGTGCCAGCGGTGCATCCGAAGGCACCATTGCGCGTCTGATCGATCTGCTGAACAGCGAACTGGCTCCTGCGGTGCCGAGCCGCGGTACCGTTGGTGCAAGCGGCGACCTGACGCCGCTGGCACATATGGTGCTGTGTCTGCAAGGCCGCGGTGATTTCCTGGATCGTGACGGTACCCGCCTGGACGGTGCAGAAGGCCTGCGTCGCGGCCGTCTGCAACCGCTGGATCTGAGCCACCGCGACGCCCTGGCACTGGTGAACGGTACCAGCGCGATGACGGGCATCGCCCTGGTTAATGCTCATGCGTGCCGTCACCTGGGTAACTGGGCAGTTGCACTGACCGCTCTGCTGGCAGAATGTCTGCGTGGTCGTACGGAAGCATGGGCGGCCGCACTGAGCGATCTGCGCCCGCATCCGGGTCAGAAAGACGCTGCGGCCCGCCTGCGTGCACGTGTTGATGGCTCCGCTCGTGTGGTTCGCCACGTCATTGCGGAACGTCGCCTGGATGCCGGTGACATCGGCACCGAACCGGAAGCCGGTCAGGATGCATACAGCCTGCGTTGCGCACCGCAAGTGCTGGGTGCTGGTTTTGATACCCTGGCGTGGCATGACCGCGTTCTGACCATTGAACTGAACGCGGTTACGGATAATCCGGTCTTTCCGCCGGACGGTAGCGTCCCGGCACTGCATGGCGGTAATTTCATGGGCCAGCACGTTGCACTGACCTCGGATGCTCTGGCAACCGCCGTTACGGTCCTGGCAGGTCTGGCAGAACGTCAAATCGCGCGCCTGACGGACGAACGTCTGAATCGCGGCCTGCCGCCGTTTCTGCATCGTGGTCCGGCGGGCCTGAACAGCGGTTTCATGGGTGCACAGGTGACCGCAACGGCTCTGCTGGCTGAAATGCGTGCAACCGGTCCGGCAAGCATTCACAGCATCAGCACGAACGCAGCTAATCAAGATGTCGTGTCGCTGGGCACCATTGCGGCCCGTCTGTGTCGCGAAAAAATCGACCGTTGGGCAGAAATTCTGGCTATCCTGGCACTGTGCCTGGCACAGGCAGCTGAACTGCGCTGTGGCAGCGGTCTGGATGGTGTCTCGCCGGCAGGCAAAAAACTGGTGCAGGCGCTGCGTGAACAATTCCCGCCGCTGGAAACCGATCGTCCGCTGGGTCAGGAAATTGCAGCACTGGCGACGCATCTGCTGCAACAAAGCCCGGTGTAaGGCTCCTTTTGGAGCCTTTTTTTTT" }, { "collection": "parts", "type": "cds", "name": "RpaI", "dnasequence": "ATGCAGGTTCATGTTATTCGTCGTGAAAATCGTGCACTGTATGCAGGTCTGCTGGAAAAATACTTTCGTATTCGCCATCAGATCTATGTTGTTGAACGTGGTTGGAAAGAACTGGATCGTCCGGATGGTCGTGAAATTGATCAGTTTGATACCGAAGATGCAGTTTATCTGCTGGGTGTTGATAACGATGATATTGTTGCAGGTATGCGTATGGTTCCGACCACCAGTCCGACCCTGCTGAGTGATGTTTTTCCGCAGCTGGCACTGGCAGGTCCGGTTCGTCGTCCTGATGCATATGAACTGAGCCGTATTTTTGTTGTTCCGCGTAAACGTGGTGAACATGGTGGTCCGCGTGCAGAAGCAGTTATTCAGGCAGCAGCAATGGAATATGGTCTGAGCATTGGTCTGAGTGCATTTACCATTGTTCTGGAAACCTGGTGGCTGCCTCGTCTGGTTGATCAGGGCTGGAAAGCAAAACCGCTGGGTCTGCCGCAGGATATTAATGGTTTTAGCACCACCGCAGTTATTGTGGATGTTGATGATGATGCATGGGTGGGTATTTGTAATCGTCGTAGCGTTCCGGGTCCGACACTGGAATGGCGTGGTCTGGAAGCAATTCGTCGTCATAGCCTGCCGGAATTTCAGGTTATTAGCTAA" }, { "collection": "parts", "type": "cds", "name": "RpaL", "dnasequence": "ATGGAGAAGGACACGAAACAAGTCGACATCATTTTTCGTTCGAAGCTCCCCGACATTTACATCCCCAACCATCTTCCGCTTCACTCGTACTGTTTTGAAAACATTTCCGAATTCAGCAGCCGGCCCTGTCTCATTAACGGCGCAAATAAGCAGATTTACACCTACGCGGATGTTGAACTTAATTCGCGCAAAGTCGCCGCCGGCCTCCATAAGCAGGGTATCCAGCCCAAAGATACGATCATGATCCTGCTTCCCAACTCGCCCGAATTCGTTTTCGCGTTTATTGGCGCGTCGTACCTGGGCGCAATCAGCACGATGGCCAACCCCCTTTTTACCCCCGCCGAGGTGGTTAAGCAAGCCAAGGCGAGCTCCGCCAAGATTATTGTCACGCAGGCATGCCACGTCAATAAAGTTAAGGATTACGCATTTGAAAATGATGTGAAGATTATCTGCATCGACAGCGCCCCCGAGGGCTGCCTGCACTTCTCGGTCCTTACGCAGGCCAATGAGCACGACATTCCCGAGGTGGAGATTCAGCCCGATGACGTCGTCGCGCTCCCCTATAGCAGCGGCACCACGGGTCTGCCCAAAGGTGTCATGCTCACCCACAAAGGCCTCGTGACCAGCGTGGCACAACAGGTGGACGGCGAAAACCCCAACCTTTACATCCATTCCGAGGACGTTATGCTGTGTGTGCTCCCCCTGTTTCACATTTACTCCCTTAACTCGGTCCTTCTTTGCGGCCTCCGTGTTGGCGCAGCAATCCTTATTATGCAAAAGTTCGATATTGTCAGCTTCCTGGAGCTCATTCAACGCTACAAAGTCACGATTGGCCCCTTCGTCCCCCCGATTGTGCTCGCGATTGCGAAGTCCCCCATGGTGGATGACTACGATCTCAGCAGCGTCCGTACGGTCATGTCCGGCGCGGCACCGCTCGGTAAAGAACTGGAGGATACGGTCCGGGCCAAATTCCCGAATGCGAAGCTGGGTCAGGGTTACGGCATGACCGAGGCAGGTCCCGTGCTCGCCATGTGCCTGGCATTCGCGAAGGAGCCCTTCGAGATCAAGAGCGGTGCGTGCGGCACGGTCGTTCGGAACGCGGAAATGAAAATCGTGGACCCCAAAACCGGCAATTCCCTTCCCCGCAACCAATCCGGCGAAATTTGCATCCGGGGTGACCAGATTATGAAGGGCTATCTTAATGACCCGGAAGCCACGGCGCGGACGATTGACAAAGAAGGTTGGCTGTATACCGGTGACATTGGTTACATCGACGATGACGACGAGCTTTTTATTGTGGACCGGCTCAAAGAACTCATCAAATATAAAGGCTTTCAAGTTGCGCCCGCGGAGCTGGAGGCACTGCTTCTTAACCATCCCAACATCTCGGATGCCGCGGTCGTGCCGATGAAGGATGAACAAGCGGGTGAAGTGCCGGTCGCGTTTGTGGTTCGGAGCAACGGCTCCACGATTACGGAGGATGAGGTTAAGGATTTTATTTCCAAGCAGGTTATCTTTTATAAACGGATTAAACGCGTTTTCTTCGTCGATGCCATCCCCAAATCCCCCAGCGGCAAGATCCTTCGGAAAGATCTTCGTGCGAAACTGGCAGCAGGCCTTCCGAATTAA" }, { "collection": "parts", "type": "cds", "name": "talS", "dnasequence": "ATGCTGGCCATGAGCCCGCCGAAACCGGCAGTGGAACTGGATCGTCATATTGATCTGGACCAGGCACACGCTGTTGCAAGCGGCGGTGCACGTATCGTCCTGGCACCTCCGGCCCGTGATCGCTGCCGTGCATCCGAAGCTCGTCTGGGTGCAGTCATTCGTGAAGCTCGCCATGTGTATGGCCTGACCACGGGTTTTGGTCCGCTGGCAAACCGTCTGATCAGCGGTGAAAATGTGCGCACCCTGCAAGCTAACCTGGTTCATCACCTGGCCAGCGGCGTGGGTCCGGTTCTGGATTGGACCACGGCACGTGCAATGGTCCTGGCACGCCTGGTGAGCATTGCACAAGGTGCCAGCGGTGCATCCGAAGGCACCATTGCGCGTCTGATCGATCTGCTGAACAGCGAACTGGCTCCTGCGGTGCCGAGCCGCGGTACCGTTGGTGCAAGCGGCGACCTGACGCCGCTGGCACATATGGTGCTGTGTCTGCAAGGCCGCGGTGATTTCCTGGATCGTGACGGTACCCGCCTGGACGGTGCAGAAGGCCTGCGTCGCGGCCGTCTGCAACCGCTGGATCTGAGCCACCGCGACGCCCTGGCACTGGTGAACGGTACCAGCGCGATGACGGGCATCGCCCTGGTTAATGCTCATGCGTGCCGTCACCTGGGTAACTGGGCAGTTGCACTGACCGCTCTGCTGGCAGAATGTCTGCGTGGTCGTACGGAAGCATGGGCGGCCGCACTGAGCGATCTGCGCCCGCATCCGGGTCAGAAAGACGCTGCGGCCCGCCTGCGTGCACGTGTTGATGGCTCCGCTCGTGTGGTTCGCCACGTCATTGCGGAACGTCGCCTGGATGCCGGTGACATCGGCACCGAACCGGAAGCCGGTCAGGATGCATACAGCCTGCGTTGCGCACCGCAAGTGCTGGGTGCTGGTTTTGATACCCTGGCGTGGCATGACCGCGTTCTGACCATTGAACTGAACGCGGTTACGGATAATCCGGTCTTTCCGCCGGACGGTAGCGTCCCGGCACTGCATGGCGGTAATTTCATGGGCCAGCACGTTGCACTGACCTCGGATGCTCTGGCAACCGCCGTTACGGTCCTGGCAGGTCTGGCAGAACGTCAAATCGCGCGCCTGACGGACGAACGTCTGAATCGCGGCCTGCCGCCGTTTCTGCATCGTGGTCCGGCGGGCCTGAACAGCGGTTTCATGGGTGCACAGGTGACCGCAACGGCTCTGCTGGCTGAAATGCGTGCAACCGGTCCGGCAAGCATTCACAGCATCAGCACGAACGCAGCTAATCAAGATGTCGTGTCGCTGGGCACCATTGCGGCCCGTCTGTGTCGCGAAAAAATCGACCGTTGGGCAGAAATTCTGGCTATCCTGGCACTGTGCCTGGCACAGGCAGCTGAACTGCGCTGTGGCAGCGGTCTGGATGGTGTCTCGCCGGCAGGCAAAAAACTGGTGCAGGCGCTGCGTGAACAATTCCCGCCGCTGGAAACCGATCGTCCGCTGGGTCAGGAAATTGCAGCACTGGCGACGCATCTGCTGCAACAAAGCCCGGTGTAA" }, { "collection": "output_devices", "name": "DAPG_out", "model": "DAPG_out_model", "structure": "DAPG_out_structure", "description": "comms_device" }, { "collection": "models", "name": "DAPG_out_model", "functions": { "response_function": "Hill_response", "input_composition": "linear_input_composition" }, "parameters": [ { "name": "ymax", "value": 0.0646, "description": "Maximal transcription (3.4 RPU * 0.019 for conversion to RNAP/s)" }, { "name": "ymin", "value": 0.00038, "description": "Minimal transcription (0.02 RPU * 0.019 for conversion to RNAP/s)" }, { "name": "kd", "value": 0.15, "description": "Dissociation equilibrium constant" }, { "name": "n", "value": 4.0, "description": "Cooperativety" }, { "name": "unit_conversion", "value": 0.102, "description": "Unit conversion" } ] }, { "collection": "structures", "name": "DAPG_out_structure", "inputs": [ { "name": "in1", "part_type": "promoter" }, { "name": "in2", "part_type": "promoter" } ], "devices": [ { "name": "DAPG_out_a", "components": [ "#in1", "#in2", "sPhl_cassette" ] } ] }, { "collection": "parts", "type": "cassette", "name": "sPhl_cassette", "dnasequence": "actcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgTACATCAGAGATTTTGAGACACAaGTACNNNNNNNNNNNNNNNNNNNNCGTAagctgtcaccggatgtgctttccggtctgatgagtccgtgaggacgaaacagcctctacaaataattttgtttaaGTGAAAGAACGGAATCTGGAGGATGTACACATGAATAAGGTAGGAATTGTGAGCTATGGCGCGGGTATCCCGGTCTGCCGGCTCAAGGTGGACGACGTGATCCAGGTGTGGAAAAACACCGATCTGAGTTTGGTAAAGGGCCAATTGGGCGTTATTGAAAGGGCCGTCCTGCAACCCGACGAAGATGTCATAACCTTGGGTGTCCTCGCCGCCCAACGCGCGCTGGACAAGGCACCACCTTGTTCCCTCGAAGCGCTTTATCTCGGCACCTGCACCAATCCTTATGACTCCCGGGCCTCGGCCGCAATCATCCTGGAAATGCTCGGCTGCGGCTATGACGCCTTTTGCGCAGACGTGCAGTTTGCCGGCAAATCAGGCACCAGCGCCCTCCAGATCGCATACGCCTTGGTAGCGTCGGGCATGGTGGGCAACGCTTTGGCGGTGGGTGCCGATACGATCAACCGCAACACCGCCCCCGGTGACTTGACGGAGTCCTATGCCGGAGCCGGGGCCGCGGCCTTGCTGTTGGGGACAGAAAATGTGATCGCGCATTTTGACGCAAGTTTTTCTTGCGCGGCGGATGTCGCTGACAACATCAGGCCTCAAGGGGACCGCTATATCCGCTCGGGAATGGGATTGGGCTCGGACAAGAACAGTATCGGCCTCGAGGACCAGACTCGCCGCGCCGCCTCGGGATTGATGGCCAAGATCCATGCGCAAGCCGATGATTTCGATTACGTCGTTTTCCAACAAAACCTGGTGTCGACCCCCTACTCTCTCGGCAAGCACCTGGGATTCACGAAGGCCCAAATCGAACCTGGCATCTACGCCCAGAGTGTCGGCGATGCTGGAGCCGCGAGTCCTTTACTGGGCTTGGTCAACGTACTCGATCAAGCGCGTCCTGGCGAAAGAATCCTGGTGGTTTCCTATGGCTTCGGTGCGGGCAGCGATGCCATCGCCTTGACCGTCACCGATGCCATCGAAGCCTATCAAAAGACCAACGTTCCGCTGCGCACGCAGCTGGAGGATAAGTACTACGTGGATTACGGGACGTCGATCAAGTACGAGTTCAAATACTTGCGGCCCGACTATGCCCTGACGGCCTATCTCTGATTCCAATCATCCAGCAAGGAGCACCTTTTTATGTGCGCACGACGCGTTGCTATCGTTTCGGCTGCCTATACCCCTAAACCTGGCAGTTCCAGAGTTCGCCAGACTTTCAAGGAGATGATCGTCGAGTCCGCCTACCAGGCACTCAACGCCATCAAGATGCACCCTCGCGAACTTCAGGCAGTTGCCTATGGGTACCATGGTGAAGGTATTTCTGAATACGGAGGTCTGGGCCCAACCATCTCCGATGCCTTGGGGATCAGCCCTGCCCCCACGTTCATGAGTACGGCCAACTGCACCAGCAGTTCGGTGTCTTTCCAGATGGCTCACCAAATGGTCGCCTCAGGTGAATACGACATCGTTCTGTGTGGCGGCTTTGAAAAAATGACCGATCACTTCAACTATGCCGAGTACATTGGCTCGAGCACTGAATGTGAATACGATTATTTTCTAGGCATCTCTCACACAGACGCCTTCGCCCTGGCGACCGCCGAATATTTTGAAAAGTACGGCTATGCAGGACGCGAAGCGGATGTATTGGCCACTTTCGGGCGGCAGATGCGCATTTATGCCCATAACACTCCCACGGCTACCCGTTATGGCGTGCCGATCCCGTCTCTCGAAACACTCAAAAGCAGCGAAGCATGCGGCTCCATGCTGGCCTGGGGTGAAGCCAGCGGTTGCGCCATCCTGGTTGCCGAACACCTTGTGCACCGCTACACGACCCAACCGGTATTTGTCCGTGGCTGTGCCTACACCGGTGTGTCGCATTATTTCGGCACGCGCTATCACAACCCGACGTTGCAATTTCCGGGGCTGCCAAAAGATGTCGGCATGGCGGTTTCCGCCAACTCGCTGGCCTGCGCCGAAATCGCCTACAAAAAAGCCGGTATCACCGCAAAGGATATCGATGTGGCGCAAGTCTACGACTTGCTGGGCGCCGGGTTGATTCAAATGGAGTCCATGGGCGTATGTGGTCCAGGCCAGGCAGGCGACTTTGTATTGGAAGGCGGCATAGCCCTTGATGGGCAACTTCCCCTGAACACTGACGGCGGCAACATCGGACGCGGCCACGCTTCCGGTTGCGATGGGATTTTGCACATTACTGAATTGTTCCGGCAGCTTCGCGGCGAGTCCAACAATCAAGTCAAAGGCGCGCGTATTGGCGTCTCGCAAAACCTGGGCGGTTACGCCGCGCACAACAGCGTCATTGTGCTTTCCAACGATTAAGGAGCCGACCATGTCCCTTTACCCTGAACAAATTCACCGCATGACCACCGCCAGCATGTTGCGCGAATGGCGCGAACACGGTGGCAAATACCGCCTCGAAGGCAGCCGATGCCAGGACTGCGAAGAAATCTTCTTCCCTCGGCGCACAGTTTGCGGTGCGTGCAACTCGTTGAATGTAGAACCGTATCGCTGCGCGCGCACCGGCACTATCCAGGTCAGCGCACACGCCGAGAACCCCATTCTTGCGGCCATGGGCTACGGCGAAACCGTGCCCCGCTTCATGGCGATGGTGCGCCTGGATGATGGTCTGGTGATTGCCTCGGAAATAGTTGACGTCATCGATCCGCGACAAGTCGTCAGCGGTGCGCCCGTGCGCATGGTCATTCGAAAACATGTCCGGGAAAGTAACTTGGCCTGGCAATATGCCTATAAGTTTGTATTGGTGAAATAACAAACTTAGACGCCGCCATACCCCGCGTTATCGATAACAACAGTCAAGTTTTTGGCCTCCAAGCCCGTTGCTTTGATTCTGTCTTTCAGAGTCCAGCTTTGCTGTTATTGCCTTAAACATGTTTCTAACTTTATTGGCTTTTAGCCGAGGACTTGTCATGTCTACTCTTTGCAAACCCAGTCTGCTGTTCCCGCAATATAAGATCACCCAGCAACAAATGATCGAGCATCTAGAGCAGTTGCATGATGATCACCCGAGAATGGCTCTTGCCAAACGCATGATTCAAAACACACAGGTGAACGAACGATACTTGGTCCTGCCCATTGATGAACTGGCGGTGCATACCGGCTTCACCCATCGCAGCATTGTTTATGAGCGCGAGGCCCGCCGCATGTCATCCATCGCGGCGCGCCAAGCCATCGAGAATGCCGGGCTGACCACCGACGACATTCGAATGGTCGCTGTGACATCGTGCACGGGTTTCATGATGCCTTCGCTGACGGCCCACTTGATCAACGACCTGGGCCTGCGAACATCGACCGTACAACTGCCTATCGCTCAATTGGGATGCGTGGCAGGCGCTGCGGCGATCAATCGAGCCAATGACTTCGCCAGCCTGGCGCCGGACAACCATGTGCTCATCGTCTCCCTGGAGTTCTCGTCGCTCTGCTATCAACCCCAGGACACCAAGTTGCACGCATTCATCTCAGCGGCATTATTCGGCGATGCGGTATCAGCCTGCGTAATGCGAGCCGACGATCAGGCGTCTGGTTTCAAGATCGCCAAGACGGGGTCTTACTTCCTGCCTGATAGCGAGCACTATATTAAATACGACGTCAAAGACAGTGGCTTTCACTTCACCCTGGACAAGGCGGTCATGAACTCCATTAAAGATGTCGCACCCATGATGGAGGAATTGAACTACGAAACCTTCAATCAACATTGCGCTCAAAATGACTTTTTCATCTTCCATACAGGCGGACGGAAAATTCTTGATGAACTGGTCCTGCAATTGGACTTGGAACCCGGCAGGGTCGCGCAATCCCGCGACAGTTTGAGCGAAGCCGGGAACATCGCCAGCGTGGTGGTTTTCGATGTGCTCAAGCGCCAGTTCGACAGCGGACCTGTCAATGGCGCGACGGGCATGTTGGCGGCCTTCGGCCCGGGTTTCACCGCTGAAATGGCCGTGGGCAAGTGGGTCGCCTGAcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttataAGGTGCTTCCTCGCTCACTGactcgctgcacgaggcagacctcagcgctagcggagtgtatactggcttactatgttggcactgatgagggtgtcagtgaagtgcttcatgtggcaggagaaaaaaggctgcaccggtgcgtcagcagaatatgtgatacaggatatattccgcttcctcgctcactgactcgctacgctcggtcgttcgactgcggcgagcggaaatggcttacgaacggggcggagatttcctggaagatgccaggaagatacttaacagggaagtgagagggccgcggcaaagccgtttttccataggctccgcccccctgacaagcatcacgaaatctgacgctcaaatcagtggtggcgaaacccgacaggactataaagataccaggcgtttccccCtggcggctccctcgtgcgctctcctgttcctgcctttcggtttaccggtgtcattccgctgttatggccgcgtttgtctcattccacgcctgacactcagttccgggtaggcagttcgctccaagctggactgtatgcacgaaccccccgttcagtccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggaaagacatgcaaaagcaccactggcagcagccactggtaattgatttagaggagttagtcttgaagtcatgcgccggttaaggctaaactgaaaggacaagttttggtgactgcgctcctccaagccagttacctcggttcaaagagttggtagctcagagaaccttcgaaaaaccgccctgcaaggcggttttttcgttttcagagcaagagattacgcgcagaccaaaacgatctcaagaagatcatcttattaaATTCTCACCAATAAAAAACGCCCGGCGGCAACCGAGCGTTCTGAACAAATCCAGATGGAGTTCTGAGGTCATTACTGGATCTATCAACAGGAGTCCAAGCGAGCTTTAGAAAAACTCATCGAGgttaggtggcggtacttgggtcgatatcaaagtgcatcacttcttcccgtatgcccaactttgtatagagagccactgcgggatcgtcaccgtaatctgcttgcacgtagatcacataagcaccaagcgcgttggcctcatgcttgaggagattgatgagcgcggtggcaatgccctgcctccggtgctcgccggagactgcgagatcatagatatagatctcactacgcggctgctcaaacttgggcagaacgtaagccgcgagagcgccaacaaccgcttcttggtcgaaggcagcaagcgcgatgaatgtcttactacggagcaagttcccgaggtaatcggagtccggctgatgttgggagtaggtggctacgtctccgaactcacgaccgaaaagatcaagagcagtccgcatggatttgacttggtcagggccgagcctacatgtgcgaatgatgcccatacttgagccacctaactttgttttagggcgactgccctgctgcgtaacatcgttgctgctgcgtaacatcgttgctgctccataacatcaaacatcgacccacggcgtaacgcgcttgctgcttggatgcccgaggcatagactgtacaaaaaaacagtcataacaagccatgaaaaccgccactgcgccgttaccaccgctgcgttcggtcaaggttctggaccagttgcgtgagcgcatacgctacttgcattacagtttacgaaccgaacaggcttatgtcaattcggttgaatactcat" }, { "collection": "parts", "type": "cds", "name": "phlA", "dnasequence": "ATGAATAAGGTAGGAATTGTGAGCTATGGCGCGGGTATCCCGGTCTGCCGGCTCAAGGTGGACGACGTGATCCAGGTGTGGAAAAACACCGATCTGAGTTTGGTAAAGGGCCAATTGGGCGTTATTGAAAGGGCCGTCCTGCAACCCGACGAAGATGTCATAACCTTGGGTGTCCTCGCCGCCCAACGCGCGCTGGACAAGGCACCACCTTGTTCCCTCGAAGCGCTTTATCTCGGCACCTGCACCAATCCTTATGACTCCCGGGCCTCGGCCGCAATCATCCTGGAAATGCTCGGCTGCGGCTATGACGCCTTTTGCGCAGACGTGCAGTTTGCCGGCAAATCAGGCACCAGCGCCCTCCAGATCGCATACGCCTTGGTAGCGTCGGGCATGGTGGGCAACGCTTTGGCGGTGGGTGCCGATACGATCAACCGCAACACCGCCCCCGGTGACTTGACGGAGTCCTATGCCGGAGCCGGGGCCGCGGCCTTGCTGTTGGGGACAGAAAATGTGATCGCGCATTTTGACGCAAGTTTTTCTTGCGCGGCGGATGTCGCTGACAACATCAGGCCTCAAGGGGACCGCTATATCCGCTCGGGAATGGGATTGGGCTCGGACAAGAACAGTATCGGCCTCGAGGACCAGACTCGCCGCGCCGCCTCGGGATTGATGGCCAAGATCCATGCGCAAGCCGATGATTTCGATTACGTCGTTTTCCAACAAAACCTGGTGTCGACCCCCTACTCTCTCGGCAAGCACCTGGGATTCACGAAGGCCCAAATCGAACCTGGCATCTACGCCCAGAGTGTCGGCGATGCTGGAGCCGCGAGTCCTTTACTGGGCTTGGTCAACGTACTCGATCAAGCGCGTCCTGGCGAAAGAATCCTGGTGGTTTCCTATGGCTTCGGTGCGGGCAGCGATGCCATCGCCTTGACCGTCACCGATGCCATCGAAGCCTATCAAAAGACCAACGTTCCGCTGCGCACGCAGCTGGAGGATAAGTACTACGTGGATTACGGGACGTCGATCAAGTACGAGTTCAAATACTTGCGGCCCGACTATGCCCTGACGGCCTATCTCTGA" }, { "collection": "parts", "type": "cds", "name": "phlC", "dnasequence": "ATGTGCGCACGACGCGTTGCTATCGTTTCGGCTGCCTATACCCCTAAACCTGGCAGTTCCAGAGTTCGCCAGACTTTCAAGGAGATGATCGTCGAGTCCGCCTACCAGGCACTCAACGCCATCAAGATGCACCCTCGCGAACTTCAGGCAGTTGCCTATGGGTACCATGGTGAAGGTATTTCTGAATACGGAGGTCTGGGCCCAACCATCTCCGATGCCTTGGGGATCAGCCCTGCCCCCACGTTCATGAGTACGGCCAACTGCACCAGCAGTTCGGTGTCTTTCCAGATGGCTCACCAAATGGTCGCCTCAGGTGAATACGACATCGTTCTGTGTGGCGGCTTTGAAAAAATGACCGATCACTTCAACTATGCCGAGTACATTGGCTCGAGCACTGAATGTGAATACGATTATTTTCTAGGCATCTCTCACACAGACGCCTTCGCCCTGGCGACCGCCGAATATTTTGAAAAGTACGGCTATGCAGGACGCGAAGCGGATGTATTGGCCACTTTCGGGCGGCAGATGCGCATTTATGCCCATAACACTCCCACGGCTACCCGTTATGGCGTGCCGATCCCGTCTCTCGAAACACTCAAAAGCAGCGAAGCATGCGGCTCCATGCTGGCCTGGGGTGAAGCCAGCGGTTGCGCCATCCTGGTTGCCGAACACCTTGTGCACCGCTACACGACCCAACCGGTATTTGTCCGTGGCTGTGCCTACACCGGTGTGTCGCATTATTTCGGCACGCGCTATCACAACCCGACGTTGCAATTTCCGGGGCTGCCAAAAGATGTCGGCATGGCGGTTTCCGCCAACTCGCTGGCCTGCGCCGAAATCGCCTACAAAAAAGCCGGTATCACCGCAAAGGATATCGATGTGGCGCAAGTCTACGACTTGCTGGGCGCCGGGTTGATTCAAATGGAGTCCATGGGCGTATGTGGTCCAGGCCAGGCAGGCGACTTTGTATTGGAAGGCGGCATAGCCCTTGATGGGCAACTTCCCCTGAACACTGACGGCGGCAACATCGGACGCGGCCACGCTTCCGGTTGCGATGGGATTTTGCACATTACTGAATTGTTCCGGCAGCTTCGCGGCGAGTCCAACAATCAAGTCAAAGGCGCGCGTATTGGCGTCTCGCAAAACCTGGGCGGTTACGCCGCGCACAACAGCGTCATTGTGCTTTCCAACGATTAA" }, { "collection": "parts", "type": "cds", "name": "phlB", "dnasequence": "ATGTCCCTTTACCCTGAACAAATTCACCGCATGACCACCGCCAGCATGTTGCGCGAATGGCGCGAACACGGTGGCAAATACCGCCTCGAAGGCAGCCGATGCCAGGACTGCGAAGAAATCTTCTTCCCTCGGCGCACAGTTTGCGGTGCGTGCAACTCGTTGAATGTAGAACCGTATCGCTGCGCGCGCACCGGCACTATCCAGGTCAGCGCACACGCCGAGAACCCCATTCTTGCGGCCATGGGCTACGGCGAAACCGTGCCCCGCTTCATGGCGATGGTGCGCCTGGATGATGGTCTGGTGATTGCCTCGGAAATAGTTGACGTCATCGATCCGCGACAAGTCGTCAGCGGTGCGCCCGTGCGCATGGTCATTCGAAAACATGTCCGGGAAAGTAACTTGGCCTGGCAATATGCCTATAAGTTTGTATTGGTGAAATAA" }, { "collection": "parts", "type": "cds", "name": "phlD", "dnasequence": "ATGTTTCTAACTTTATTGGCTTTTAGCCGAGGACTTGTCATGTCTACTCTTTGCAAACCCAGTCTGCTGTTCCCGCAATATAAGATCACCCAGCAACAAATGATCGAGCATCTAGAGCAGTTGCATGATGATCACCCGAGAATGGCTCTTGCCAAACGCATGATTCAAAACACACAGGTGAACGAACGATACTTGGTCCTGCCCATTGATGAACTGGCGGTGCATACCGGCTTCACCCATCGCAGCATTGTTTATGAGCGCGAGGCCCGCCGCATGTCATCCATCGCGGCGCGCCAAGCCATCGAGAATGCCGGGCTGACCACCGACGACATTCGAATGGTCGCTGTGACATCGTGCACGGGTTTCATGATGCCTTCGCTGACGGCCCACTTGATCAACGACCTGGGCCTGCGAACATCGACCGTACAACTGCCTATCGCTCAATTGGGATGCGTGGCAGGCGCTGCGGCGATCAATCGAGCCAATGACTTCGCCAGCCTGGCGCCGGACAACCATGTGCTCATCGTCTCCCTGGAGTTCTCGTCGCTCTGCTATCAACCCCAGGACACCAAGTTGCACGCATTCATCTCAGCGGCATTATTCGGCGATGCGGTATCAGCCTGCGTAATGCGAGCCGACGATCAGGCGTCTGGTTTCAAGATCGCCAAGACGGGGTCTTACTTCCTGCCTGATAGCGAGCACTATATTAAATACGACGTCAAAGACAGTGGCTTTCACTTCACCCTGGACAAGGCGGTCATGAACTCCATTAAAGATGTCGCACCCATGATGGAGGAATTGAACTACGAAACCTTCAATCAACATTGCGCTCAAAATGACTTTTTCATCTTCCATACAGGCGGACGGAAAATTCTTGATGAACTGGTCCTGCAATTGGACTTGGAACCCGGCAGGGTCGCGCAATCCCGCGACAGTTTGAGCGAAGCCGGGAACATCGCCAGCGTGGTGGTTTTCGATGTGCTCAAGCGCCAGTTCGACAGCGGACCTGTCAATGGCGCGACGGGCATGTTGGCGGCCTTCGGCCCGGGTTTCACCGCTGAAATGGCCGTGGGCAAGTGGGTCGCCTGA" }, { "collection": "parts", "type": "cds", "name": "yfp", "dnasequence": "ATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACAGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCTTCGGCTACGGCCTGCAATGCTTCGCCCGCTACCCCGACCACATGAAGCTGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCAATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTTAGCTACCAGTCCGCCCTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTGTACAAGTAA" }, { "collection": "parts", "type": "cds", "name": "rfp", "dnasequence": "ATGGCTTCCTCCGAAGACGTTATCAAAGAGTTCATGCGTTTCAAAGTTCGTATGGAAGGTTCCGTTAACGGTCACGAGTTCGAAATCGAAGGTGAAGGTGAAGGTCGTCCGTACGAAGGTACCCAGACCGCTAAACTGAAAGTTACCAAAGGTGGTCCGCTGCCGTTCGCTTGGGACATCCTGTCCCCGCAGTTCCAGTACGGTTCCAAAGCTTACGTTAAACACCCGGCTGACATCCCGGACTACCTGAAACTGTCCTTCCCGGAAGGTTTCAAATGGGAACGTGTTATGAACTTCGAAGACGGTGGTGTTGTTACCGTTACCCAGGACTCCTCCCTGCAAGACGGTGAGTTCATCTACAAAGTTAAACTGCGTGGTACCAACTTCCCGTCCGACGGTCCGGTTATGCAGAAAAAAACCATGGGTTGGGAAGCTTCCACCGAACGTATGTACCCGGAAGACGGTGCTCTGAAAGGTGAAATCAAAATGCGTCTGAAACTGAAAGACGGTGGTCACTACGACGCTGAAGTTAAAACCACCTACATGGCTAAAAAACCGGTTCAGCTGCCGGGTGCTTACAAAACCGACATCAAACTGGACATCACCTCCCACAACGAAGACTACACCATCGTTGAACAGTACGAACGTGCTGAAGGTCGTCACTCCACCGGTGCTTAA" }, { "collection": "parts", "type": "cds", "name": "bfp", "dnasequence": "ATGGTGTCTAAGGGCGAAGAGCTGATTAAGGAGAACATGCACATGAAGCTGTACATGGAGGGCACCGTGGACAACCATCACTTCAAGTGCACATCCGAGGGCGAAGGCAAGCCCTACGAGGGCACCCAGACCATGCGCATCAAGGTGGTCGAGGGCGGCCCTCTCCCCTTCGCCTTCGACATCCTGGCTACTAGCTTCCTCTACGGCAGCAAGACCTTCATCAACCACACCCAGGGCATCCCCGACTTCTTCAAGCAGTCCTTCCCTGAGGGCTTCACATGGGAGCGCGTCACCACATACGAGGACGGGGGCGTGCTGACCGCTACCCAGGACACCAGCCTCCAGGACGGCTGCCTCATCTACAACGTCAAGATCCGGGGGGTGAACTTCACATCCAACGGCCCTGTGATGCAGAAGAAAACACTCGGCTGGGAGGCCTTCACCGAAACCCTGTACCCCGCTGACGGCGGCCTGGAAGGCCGTAACGACATGGCCCTGAAGCTCGTGGGCGGGAGCCATCTGATCGCAAACGCCAAGACCACATATCGTTCCAAGAAACCCGCTAAGAACCTCAAGATGCCTGGCGTCTACTATGTGGACTACCGCCTGGAACGTATCAAGGAGGCCAACAACGAAACGTACGTCGAGCAGCACGAGGTGGCAGTGGCCCGGTACTGCGACCTCCCTAGCAAACTGGGGCACAAGCTTAATTAA" }, { "collection": "parts", "type": "cds", "name": "cas9", "dnasequence": "ATGGATAAGAAATACTCAATAGGCTTAGATATCGGCACAAATAGCGTCGGATGGGCGGTGATCACTGATGAATATAAGGTTCCGTCTAAAAAGTTCAAGGTTCTGGGAAATACAGACCGCCACAGTATCAAAAAAAATCTTATAGGGGCTCTTTTATTTGACAGTGGAGAGACAGCGGAAGCGACTCGTCTCAAACGGACAGCTCGTAGAAGGTATACACGTCGGAAGAATCGTATTTGTTATCTACAGGAGATTTTTTCAAATGAGATGGCGAAAGTAGATGATAGTTTCTTTCATCGACTTGAAGAGTCTTTTTTGGTGGAAGAAGACAAGAAGCATGAACGTCATCCTATTTTTGGAAATATAGTAGATGAAGTTGCTTATCATGAGAAATATCCAACTATCTATCATCTGCGAAAAAAATTGGTAGATTCTACTGATAAAGCGGATTTGCGCTTAATCTATTTGGCCTTAGCGCATATGATTAAGTTTCGTGGTCATTTTTTGATTGAGGGAGATTTAAATCCTGATAATAGTGATGTGGACAAACTATTTATCCAGTTGGTACAAACCTACAATCAATTATTTGAAGAAAACCCTATTAACGCAAGTGGAGTAGATGCTAAAGCGATTCTTTCTGCACGATTGAGTAAATCAAGACGATTAGAAAATCTCATTGCTCAGCTCCCCGGTGAGAAGAAAAATGGCTTATTTGGGAATCTCATTGCTTTGTCATTGGGTTTGACCCCTAATTTTAAATCAAATTTTGATTTGGCAGAAGATGCTAAATTACAGCTTTCAAAAGATACTTACGATGATGATTTAGATAATTTATTGGCGCAAATTGGAGATCAATATGCTGATTTGTTTTTGGCAGCTAAGAATTTATCAGATGCTATTTTACTTTCAGATATCCTAAGAGTAAATACTGAAATAACTAAGGCTCCCCTATCAGCTTCAATGATTAAACGCTACGATGAACATCATCAAGACTTGACTCTTTTAAAAGCTTTAGTTCGACAACAACTTCCAGAAAAGTATAAAGAAATCTTTTTTGATCAATCAAAAAACGGATATGCAGGTTATATTGATGGGGGAGCTAGCCAAGAAGAATTTTATAAATTTATCAAACCAATTTTAGAAAAAATGGATGGTACTGAGGAATTATTGGTGAAACTAAATCGTGAAGATTTGCTGCGCAAGCAACGGACCTTTGACAACGGCTCTATTCCCCATCAAATTCACTTGGGTGAGCTGCATGCTATTTTGAGAAGACAAGAAGACTTTTATCCATTTTTAAAAGACAATCGTGAGAAGATTGAAAAAATCTTGACTTTTCGAATTCCTTATTATGTTGGTCCATTGGCGCGTGGCAATAGTCGTTTTGCATGGATGACTCGGAAGTCTGAAGAAACAATTACCCCATGGAATTTTGAAGAAGTTGTCGATAAAGGTGCTTCAGCTCAATCATTTATTGAACGCATGACAAACTTTGATAAAAATCTTCCAAATGAAAAAGTACTACCAAAACATAGTTTGCTTTATGAGTATTTTACGGTTTATAACGAATTGACAAAGGTCAAATATGTTACTGAAGGAATGCGAAAACCAGCATTTCTTTCAGGTGAACAGAAGAAAGCCATTGTTGATTTACTCTTCAAAACAAATCGAAAAGTAACCGTTAAGCAATTAAAAGAAGATTATTTCAAAAAAATAGAATGTTTTGATAGTGTTGAAATTTCAGGAGTTGAAGATAGATTTAATGCTTCATTAGGTACCTACCATGATTTGCTAAAAATTATTAAAGATAAAGATTTTTTGGATAATGAAGAAAATGAAGATATCTTAGAGGATATTGTTTTAACATTGACCTTATTTGAAGATAGGGAGATGATTGAGGAAAGACTTAAAACATATGCTCACCTCTTTGATGATAAGGTGATGAAACAGCTTAAACGTCGCCGTTATACTGGTTGGGGACGTTTGTCTCGAAAATTGATTAATGGTATTAGGGATAAGCAATCTGGCAAAACAATATTAGATTTTTTGAAATCAGATGGTTTTGCCAATCGCAATTTTATGCAGCTGATCCATGATGATAGTTTGACATTTAAAGAAGACATTCAAAAAGCACAAGTGTCTGGACAAGGCGATAGTTTACATGAACATATTGCAAATTTAGCTGGTAGCCCTGCTATTAAAAAAGGTATTTTACAGACTGTAAAAGTTGTTGATGAATTGGTCAAAGTAATGGGGCGGCATAAGCCAGAAAATATCGTTATTGAAATGGCACGTGAAAATCAGACAACTCAAAAGGGCCAGAAAAATTCGCGAGAGCGTATGAAACGAATCGAAGAAGGTATCAAAGAATTAGGAAGTCAGATTCTTAAAGAGCATCCTGTTGAAAATACTCAATTGCAAAATGAAAAGCTCTATCTCTATTATCTCCAAAATGGAAGAGACATGTATGTGGACCAAGAATTAGATATTAATCGTTTAAGTGATTATGATGTCGATCACATTGTTCCACAAAGTTTCCTTAAAGACGATTCAATAGACAATAAGGTCTTAACGCGTTCTGATAAAAATCGTGGTAAATCGGATAACGTTCCAAGTGAAGAAGTAGTCAAAAAGATGAAAAACTATTGGAGACAACTTCTAAACGCCAAGTTAATCACTCAACGTAAGTTTGATAATTTAACGAAAGCTGAACGTGGAGGTTTGAGTGAACTTGATAAAGCTGGTTTTATCAAACGCCAATTGGTTGAAACTCGCCAAATCACTAAGCATGTGGCACAAATTTTGGATAGTCGCATGAATACTAAATACGATGAAAATGATAAACTTATTCGAGAGGTTAAAGTGATTACCTTAAAATCTAAATTAGTTTCTGACTTCCGAAAAGATTTCCAATTCTATAAAGTACGTGAGATTAACAATTACCATCATGCCCATGATGCGTATCTAAATGCCGTCGTTGGAACTGCTTTGATTAAGAAATATCCAAAACTTGAATCGGAGTTTGTCTATGGTGATTATAAAGTTTATGATGTTCGTAAAATGATTGCTAAGTCTGAGCAAGAAATAGGCAAAGCAACCGCAAAATATTTCTTTTACTCTAATATCATGAACTTCTTCAAAACAGAAATTACACTTGCAAATGGAGAGATTCGCAAACGCCCTCTAATCGAAACTAATGGGGAAACTGGAGAAATTGTCTGGGATAAAGGGCGAGATTTTGCCACAGTGCGCAAAGTATTGTCCATGCCCCAAGTCAATATTGTCAAGAAAACAGAAGTACAGACAGGCGGATTCTCCAAGGAGTCAATTTTACCAAAAAGAAATTCGGACAAGCTTATTGCTCGTAAAAAAGACTGGGATCCAAAAAAATATGGTGGTTTTGATAGTCCAACGGTAGCTTATTCAGTCCTAGTGGTTGCTAAGGTGGAAAAAGGGAAATCGAAGAAGTTAAAATCCGTTAAAGAGTTACTAGGGATCACAATTATGGAAAGAAGTTCCTTTGAAAAAAATCCGATTGACTTTTTAGAAGCTAAAGGATATAAGGAAGTTAAAAAAGACTTAATCATTAAACTACCTAAATATAGTCTTTTTGAGTTAGAAAACGGTCGTAAACGGATGCTGGCTAGTGCCGGAGAATTACAAAAAGGAAATGAGCTGGCTCTGCCAAGCAAATATGTGAATTTTTTATATTTAGCTAGTCATTATGAAAAGTTGAAGGGTAGTCCAGAAGATAACGAACAAAAACAATTGTTTGTGGAGCAGCATAAGCATTATTTAGATGAGATTATTGAGCAAATCAGTGAATTTTCTAAGCGTGTTATTTTAGCAGATGCCAATTTAGATAAAGTTCTTAGTGCATATAACAAACATAGAGACAAACCAATACGTGAACAAGCAGAAAATATTATTCATTTATTTACGTTGACGAATCTTGGAGCTCCCGCTGCTTTTAAATATTTTGATACAACAATTGATCGTAAACGATATACGTCTACAAAAGAAGTTTTAGATGCCACTCTTATCCATCAATCCATCACTGGTCTTTATGAAACACGCATTGATTTGAGTCAGCTAGGAGGTGACTGA" }, { "collection": "parts", "type": "cds", "name": "gam", "dnasequence": "CTAATCAGCCCGGCATTTCGCGGGCGATATTTTCACAGCTATTTCAGGAGTTCAGCCATGAACGCTTATTACATTCAGGATCGTCTTGAGGCTCAGAGCTGGGCGCGTCACTACCAGCAGCTCGCCCGTGAAGAGAAAGAGGCAGAACTGGCAGACGACATGGAAAAAGGCCTGCCCCAGCACCTGTTTGAATCGCTATGCATCGATCATTTGCAACGCCACGGGGCCAGCAAAAAATCCATTACCCGTGCGTTTGATGACGATGTTGAGTTTCAGGAGCGCATGGCAGAACACATCCGGTACATGGTTGAAACCATTGCTCACCACCAGGTTGATATTGATTCAGAGGTATAA" }, { "collection": "parts", "type": "cds", "name": "gam", "dnasequence": "ATGAGTACTGCACTCGCAACGCTGGCTGGGAAGCTGGCTGAACGTGTCGGCATGGATTCTGTCGACCCACAGGAACTGATCACCACTCTTCGCCAGACGGCATTTAAAGGTGATGCCAGCGATGCGCAGTTCATCGCATTACTGATCGTTGCCAACCAGTACGGCCTTAATCCGTGGACGAAAGAAATTTACGCCTTTCCTGATAAGCAGAATGGCATCGTTCCGGTGGTGGGCGTTGATGGCTGGTCCCGCATCATCAATGAAAACCAGCAGTTTGATGGCATGGACTTTGAGCAGGACAATGAATCCTGTACATGCCGGATTTACCGCAAGGACCGTAATCATCCGATCTGCGTTACCGAATGGATGGATGAATGCCGCCGCGAACCATTCAAAACTCGCGAAGGCAGAGAAATCACGGGGCCGTGGCAGTCGCATCCCAAACGGATGTTACGTCATAAAGCCATGATTCAGTGTGCCCGTCTGGCCTTCGGATTTGCTGGTATCTATGACAAGGATGAAGCCGAGCGCATTGTCGAAAATACTGCATACACTGCAGAACGTCAGCCGGAACGCGACATCACTCCGGTTAACGATGAAACCATGCAGGAGATTAACACTCTGCTGATCGCCCTGGATAAAACATGGGATGACGACTTATTGCCGCTCTGTTCCCAGATATTTCGCCGCGACATTCGTGCATCGTCAGAACTGACACAGGCCGAAGCAGTAAAAGCTCTTGGATTCCTGAAACAGAAAGCCGCAGAGCAGAAGGTGGCAGCATGA" }, { "collection": "parts", "type": "cds", "name": "exo", "dnasequence": "ATGACACCGGACATTATCCTGCAGCGTACCGGGATCGATGTGAGAGCTGTCGAACAGGGGGAAGATGCGTGGCACAAATTACGGCTCGGCGTCATCACCGCTTCAGAAGTTCACAACGTGATAGCAAAACCCCGCTCCGGAAAGAAGTGGCCTGACATGAAAATGTCCTACTTCCACACCCTGCTTGCTGAGGTTTGCACCGGTGTGGCTCCGGAAGTTAACGCTAAAGCACTGGCCTGGGGAAAACAGTACGAGAACGACGCCAGAACCCTGTTTGAATTTACTTCCGGCGTGAATGTTACTGAATCCCCGATCATCTATCGCGACGAAAGTATGCGTACCGCCTGCTCTCCCGATGGTTTATGCAGTGACGGCAACGGCCTTGAACTGAAATGCCCGTTTACCTCCCGGGATTTCATGAAGTTCCGGCTCGGTGGTTTCGAGGCCATAAAGTCAGCTTACATGGCCCAGGTGCAGTACAGCATGTGGGTGACGCGAAAAAATGCCTGGTACTTTGCCAACTATGACCCGCGTATGAAGCGTGAAGGCCTGCATTATGTCGTGATTGAGCGGGATGAAAAGTACATGGCGAGTTTTGACGAGATCGTGCCGGAGTTCATCGAAAAAATGGACGAGGCACTGGCTGAAATTGGTTTTGTATTTGGGGAGCAATGGCGATGA" }, { "collection": "parts", "type": "cds", "name": "hsvtk", "dnasequence": "ATGGCGAGCTATCCGTGTCACCAGCATGCATCTGCTTTCGATCAGGCAGCGCGCAGCCGTGGTCATTCTAATCGTCGTACCGCACTGCGTCCGCGTCGTCAGCAGGAGGCCACTGAGGTTCGTCTGGAGCAAAAGATGCCGACCCTGTTACGCGTATACATTGATGGGCCGCATGGTATGGGTAAAACCACCACGACCCAATTACTGGTTGCGCTGGGCAGCCGTGATGATATTGTTTATGTGCCTGAACCGATGACGTATTGGCAGGTGCTGGGCGCGAGTGAAACTATTGCTAATATCTATACGACCCAGCATCGTCTGGACCAAGGGGAAATCAGCGCCGGTGATGCAGCCGTAGTGATGACCAGTGCGCAAATCACGATGGGTATGCCTTACGCAGTAACCGATGCGGTTCTGGCGCCGCATATTGGTGGTGAAGCCGGCAGTAGCCATGCGCCGCCGCCTGCCCTGACCCTGATTTTTGATCGTCACCCGATTGCGGCTCTGCTGTGCTATCCTGCTGCACGTTATCTGATGGGTTCTATGACCCCACAGGCCGTCCTGGCATTCGTTGCACTGATTCCGCCTACTCTGCCTGGGACCAATATCGTGCTGGGGGCGCTGCCAGAAGATCGTCATATCGACCGTCTGGCGAAACGTCAACGTCCTGGTGAACGCCTGGATCTGGCGATGCTGGCAGCGATTCGTCGTGTATATGGCCTGCTGGCGAACACTGTCCGTTACCTGCAAGGCGGTGGCAGTTGGCGTGAAGATTGGGGTCAACTGAGCGGTACGGCAGTTCCTCCGCAGGGTGCGGAACCTCAGTCTAACGCAGGTCCGCGTCCGCACATTGGTGATACCCTGTTCACCCTGTTCCGTGCGCCGGAGCTGCTGGCACCAAATGGGGATCTGTACAATGTTTTCGCGTGGGCGCTGGATGTTCTGGCTAAGCGTCTGCGCCCGATGCATGTTTTTATTCTGGATTATGATCAAAGCCCAGCAGGCTGTCGTGATGCGCTGCTTCAACTGACTAGCGGCATGGTGCAAACGCATGTGACGACGCCTGGGAGTATCCCGACCATCTGTGATCTTGCCCGTACCTTCGCACGTGAAATGGGTGAAGCGAATTAA" }, { "collection": "parts", "type": "cds", "name": "kanr", "dnasequence": "ATGAGCCATATTCAACGGGAAACGTCTTGCTCTAGGCCGCGATTAAATTCCAACATGGATGCTGATTTATATGGGTATAAATGGGCTCGCGATAATGTCGGGCAATCAGGTGCGACAATCTATCGATTGTATGGGAAGCCCGATGCGCCAGAGTTGTTTCTGAAACATGGCAAAGGTAGCGTTGCCAATGATGTTACAGATGAGATGGTCAGACTAAACTGGCTGACGGAATTTATGCCTCTTCCGACCATCAAGCATTTTATCCGTACTCCTGATGATGCATGGTTACTCACCACTGCGATCCCCGGGAAAACAGCATTCCAGGTATTAGAAGAATATCCTGATTCAGGTGAAAATATTGTTGATGCGCTGGCAGTGTTCCTGCGCCGGTTGCATTCGATTCCTGTTTGTAATTGTCCTTTTAACAGCGACCGCGTATTTCGTCTCGCTCAGGCGCAATCACGAATGAATAACGGTTTGGTTGATGCGAGTGATTTTGATGACGAGCGTAATGGCTGGCCTGTTGAACAAGTCTGGAAAGAAATGCATAAACTTTTGCCATTCTCACCGGATTCAGTCGTCACTCATGGTGATTTCTCACTTGATAACCTTATTTTTGACGAGGGGAAATTAATAGGTTGTATTGATGTTGGACGAGTCGGAATCGCAGACCGATACCAGGATCTTGCCATCCTATGGAACTGCCTCGGTGAGTTTTCTCCTTCATTACAGAAACGGCTTTTTCAAAAATATGGTATTGATAATCCTGATATGAATAAATTGCAGTTTCATTTGATGCTCGATGAGTTTTTCTAA" }, { "collection": "parts", "type": "cds", "name": "ampr", "dnasequence": "ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTATATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGTTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAA" }, { "collection": "parts", "type": "cds", "name": "tetA", "dnasequence": "ATGAAATCTAACAATGCGCTCATCGTCATCCTCGGCACCGTCACCCTGGATGCTGTAGGCATAGGCTTGGTTATGCCGGTACTGCCGGGCCTCTTGCGGGATATCGTCCATTCCGACAGCATCGCCAGTCACTATGGCGTGCTGCTAGCGCTATATGCGTTGATGCAATTTCTATGCGCACCCGTTCTCGGAGCACTGTCCGACCGCTTTGGCCGCCGCCCAGTCCTGCTCGCTTCGCTACTTGGAGCCACTATCGACTACGCGATCATGGCGACCACACCCGTCCTGTGGATTCTCTACGCCGGACGCATCGTGGCCGGCATCACCGGCGCCACAGGTGCGGTTGCTGGCGCCTATATCGCCGACATCACCGATGGGGAAGATCGGGCTCGCCACTTCGGGCTCATGAGCGCTTGTTTCGGCGTGGGTATGGTGGCAGGCCCCGTGGCCGGGGGACTGTTGGGCGCCATCTCCCTGCACGCACCATTCCTTGCGGCGGCGGTGCTCAACGGCCTCAACCTACTACTGGGCTGCTTCCTAATGCAGGAGTCGCATAAGGGAGAGCGCCGTCCGATGCCCTTGAGAGCCTTCAACCCAGTCAGCTCCTTCCGGTGGGCGCGGGGCATGACCATTGTGGCCGCACTTATGACTGTCTTCTTTATCATGCAACTCGTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGCGCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCCTTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATGGCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTCCCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTGTCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACCAGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGCCTCGGCGAGCACATGGAACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGTCGCGGTGCATGGAGCCGGGCCACCTCGACCTGA" }, { "collection": "parts", "type": "cds", "name": "gmR", "dnasequence": "ATGTTACGCAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAGGTGGCTCAAGTATGGGCATCATTCGCACATGTAGGCTCGGCCCTGACCAAGTCAAATCCATGCGGACTGCTCTTGATCTTTTCGGTCGTGAGTTCGGAGACGTAGCCACCTACTCCCAACATCAGCCGGACTCCGATTACCTCGGGAACTTGCTCCGTAGTAAGACATTCATCGCGCTTGCTGCCTTCGACCAAGAAGCGGTTGTTGGCGCTCTCGCGGCTTACGTTCTGCCCAAGTTTGAGCAGCCGCGTAGTGAGATCTATATCTATGATCTCGCAGTCTCCGGCGAGCACCGGAGGCAGGGCATTGCCACCGCGCTCATCAATCTCCTCAAGCATGAGGCCAACGCGCTTGGTGCTTATGTGATCTACGTGCAAGCAGATTACGGTGACGATCCCGCAGTGGCTCTCTATACAAAGTTGGGCATACGGGAAGAAGTGATGCACTTTGATATCGACCCAAGTACCGCCACCTAA" }, { "collection": "parts", "type": "cds", "name": "specR", "dnasequence": "ATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCACAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAA" }, { "collection": "functions", "name": "Hill_response", "equation": "ymin + (ymax - ymin) / (1.0 + (kd / (unit_conversion * x))^n)", "variables": [ { "name": "x", "map": "#//model/functions/input_composition" } ], "parameters": [ { "name": "ymax", "map": "#//model/parameters/ymax" }, { "name": "ymin", "map": "#//model/parameters/ymin" }, { "name": "kd", "map": "#//model/parameters/kd" }, { "name": "n", "map": "#//model/parameters/n" } ] }, { "collection": "functions", "name": "linear_input_composition", "equation": "x", "variables": [ { "name": "x", "map": "#//structure/inputs/in1/model/functions/response_function" } ] }, { "collection": "circuit_rules", "rules": { "function": "AND", "rules": [ "OC6_out_a AFTER attB5", "OHC12_out_a AFTER attB5", "DAPG_out_a AFTER attB5", "pC_HSL_out_a AFTER attB5", "ALL_FORWARD" ] } }, { "collection": "device_rules", "rules": { "function": "AND", "rules": [ "ALL_FORWARD" ] } } ]