[ { "collection": "header", "description": "cI homologs: JR1, JR2, JR3, JR4, JR5, JR6, JR7, JR12, N15, JR8, JR9, JR10, JR11", "version": "Eco2C1G5T1", "date": "Tue April 13 16:01:11 EDT 2021", "author": [ "Jai Padmakumar", "Alexander J. Triassi", "Hamid Doosthosseini", "Samuel M.D. Oliveira" ], "organism": "Escherichia coli MG1655 (Extended #Gates))", "genome": "Wildtype (NCBI RefSeq: CP007799) with three landing pads integrated", "media": "M9 minimal media composed of M9 media salts (6.78 g/L Na2HPO4, 3 g/L KH2PO4, 1 g/L NH4Cl, 0.5 g/L NaCl, 0.34 g/L thiamine hydrochloride, 0.4% D-glucose, 0.2% Casamino acids, 2 mM MgSO4, and 0.1 mM CaCl2", "temperature": "37", "growth": "Inoculation: Individual colonies into M9 media, 16 hours overnight in plate shaker.\tDilution: Next day, cells diluted 150-fold into M9 media without antibiotics, growth for 1.5 hours.\tInduction: Cells diluted into M9 media without antibiotics (starting OD600 = 0.0033).\tGrowth: shaking incubator for 5 hours.\tArrest protein production: PBS and 1 mg/ml kanamycin. Measurement: flow cytometry, data processing for RPU normalization." }, { "collection": "measurement_std", "signal_carrier_units": "RPUG", "normalization_instructions": "The following equation converts the median YFP fluorescence to RPU. RPU = (YFP - YFP0)/(YFPRPUG - YFP0), where YFP is the median fluorescence of the cells of interest, YFP0 is the median fluorescence of the cells containing full sensor array with two other landing pads empty, and YFPRPUG is the median fluorescence of the cells containing the measurement standard on the genome landing pad", "plasmid_description": "Genome integrated RPU standard where YFP fluorescent protein is driven by J23101 promoter and B0034 RBS. RiboJ was used as a ribozyme insulator. A bidirectional terminator DT3 was used to insulate YFP from the genome. The insertion is in the landing pad #2, at the position of 4633382" }, { "collection": "input_composition", "signal_carrier_units": "RPUG", "normalization_instructions": "The following equation converts the median YFP fluorescence to RPU. RPU = (YFP - YFP0)/(YFPRPUG - YFP0), where YFP is the median fluorescence of the cells of interest, YFP0 is the median fluorescence of the cells containing full sensor array with two other landing pads empty, and YFPRPUG is the median fluorescence of the cells containing the measurement standard on the genome landing pad.", "plasmid_description": "We used the pRpa_A (pC_HSL inducer) sensor cassette, which was cloned alongside the circuit. The DNA sequence of this particular input sensor includes a promoter, ribozyme, RBS, sensor protein, and terminator (all required components)" }, { "collection": "genetic_locations", "locations": [ { "symbol": "attB2", "description": "", "locus": { "start": 0, "end": 0 }, "sequence": { "type": "", "ref": "" } }, { "symbol": "attB7", "description": "", "locus": { "start": 0, "end": 0 }, "sequence": { "type": "", "ref": "" } }, { "symbol": "attB5", "description": "", "locus": { "start": 0, "end": 0 }, "sequence": { "type": "", "ref": "" } } ] }, { "collection": "logic_constraints", "available_gates": [ { "type": "NOR", "max_instances": 12 }, { "type": "OUTPUT_OR", "max_instances": true } ] }, { "collection": "gates", "name": "Rj13_JR1", "system": "TetR", "group": "JR1", "regulator": "JR1", "gate_type": "NOR", "color": "F9A427", "model": "Rj13_JR1_model", "structure": "Rj13_JR1_structure" }, { "collection": "gates", "name": "Rj14_JR1", "system": "TetR", "group": "JR1", "regulator": "JR1", "gate_type": "NOR", "color": "3BA9E0", "model": "Rj14_JR1_model", "structure": "Rj14_JR1_structure" }, { "collection": "gates", "name": "Rj21_JR2", "system": "TetR", "group": "JR2", "regulator": "JR2", "gate_type": "NOR", "color": "EE2F2B", "model": "Rj21_JR2_model", "structure": "Rj21_JR2_structure" }, { "collection": "gates", "name": "Rj31_JR3", "system": "TetR", "group": "JR3", "regulator": "JR3", "gate_type": "NOR", "color": "6FCDE1", "model": "Rj31_JR3_model", "structure": "Rj31_JR3_structure" }, { "collection": "gates", "name": "Rj41_JR4", "system": "TetR", "group": "JR4", "regulator": "JR4", "gate_type": "NOR", "color": "C24B9C", "model": "Rj41_JR4_model", "structure": "Rj41_JR4_structure" }, { "collection": "gates", "name": "Rj71_JR7", "system": "TetR", "group": "JR7", "regulator": "JR7", "gate_type": "NOR", "color": "3A563F", "model": "Rj71_JR7_model", "structure": "Rj71_JR7_structure" }, { "collection": "gates", "name": "Rj72_JR7", "system": "TetR", "group": "JR7", "regulator": "JR7", "gate_type": "NOR", "color": "9635CF", "model": "Rj72_JR7_model", "structure": "Rj72_JR7_structure" }, { "collection": "gates", "name": "Rj81_JR8", "system": "TetR", "group": "JR8", "regulator": "JR8", "gate_type": "NOR", "color": "261239", "model": "Rj81_JR8_model", "structure": "Rj81_JR8_structure" }, { "collection": "gates", "name": "Rj91_JR9", "system": "TetR", "group": "JR9", "regulator": "JR9", "gate_type": "NOR", "color": "CA30C6", "model": "Rj91_JR9_model", "structure": "Rj91_JR9_structure" }, { "collection": "gates", "name": "Rj92_JR9", "system": "TetR", "group": "JR9", "regulator": "JR9", "gate_type": "NOR", "color": "08A297", "model": "Rj92_JR9_model", "structure": "Rj92_JR9_structure" }, { "collection": "gates", "name": "Rj93_JR9", "system": "TetR", "group": "JR9", "regulator": "JR9", "gate_type": "NOR", "color": "81BC02", "model": "Rj93_JR9_model", "structure": "Rj93_JR9_structure" }, { "collection": "gates", "name": "Rj111_JR11", "system": "TetR", "group": "JR11", "regulator": "JR11", "gate_type": "NOR", "color": "D3799B", "model": "Rj111_JR11_model", "structure": "Rj111_JR11_structure" }, { "collection": "structures", "name": "Rj13_JR1_structure", "inputs": [ { "name": "in1", "part_type": "promoter" }, { "name": "in2", "part_type": "promoter" } ], "outputs": [ "pJR1" ], "devices": [ { "name": "Rj13_JR1_a", "components": [ "#in1", "Rj13_JR1_a_cassette" ] }, { "name": "Rj13_JR1_a_cassette", "components": [ "RiboJ54", "Rj13", "JR1", "Ret0" ] }, { "name": "Rj13_JR1_b", "components": [ "#in2", "Rj13_JR1_b_cassette" ] }, { "name": "Rj13_JR1_b_cassette", "components": [ "RiboJ54", "Rj13", "JR1", "Ret0" ] } ] }, { "collection": "structures", "name": "Rj14_JR1_structure", "inputs": [ { "name": "in1", "part_type": "promoter" }, { "name": "in2", "part_type": "promoter" } ], "outputs": [ "pJR1" ], "devices": [ { "name": "Rj14_JR1_a", "components": [ "#in1", "Rj14_JR1_a_cassette" ] }, { "name": "Rj14_JR1_a_cassette", "components": [ "RiboJ54", "Rj14", "JR1", "Ret0" ] }, { "name": "Rj14_JR1_b", "components": [ "#in2", "Rj14_JR1_b_cassette" ] }, { "name": "Rj14_JR1_b_cassette", "components": [ "RiboJ54", "Rj14", "JR1", "Ret0" ] } ] }, { "collection": "structures", "name": "Rj21_JR2_structure", "inputs": [ { "name": "in1", "part_type": "promoter" }, { "name": "in2", "part_type": "promoter" } ], "outputs": [ "pJR2" ], "devices": [ { "name": "Rj21_JR2_a", "components": [ "#in1", "Rj21_JR2_a_cassette" ] }, { "name": "Rj21_JR2_a_cassette", "components": [ "RiboJ71", "Rj21", "JR2", "IlvGEDA" ] }, { "name": "Rj21_JR2_b", "components": [ "#in2", "Rj21_JR2_b_cassette" ] }, { "name": "Rj21_JR2_b_cassette", "components": [ "RiboJ71", "Rj21", "JR2", "IlvGEDA" ] } ] }, { "collection": "structures", "name": "Rj31_JR3_structure", "inputs": [ { "name": "in1", "part_type": "promoter" }, { "name": "in2", "part_type": "promoter" } ], "outputs": [ "pJR3" ], "devices": [ { "name": "Rj31_JR3_a", "components": [ "#in1", "Rj31_JR3_a_cassette" ] }, { "name": "Rj31_JR3_a_cassette", "components": [ "RiboJ69", "Rj31", "JR3", "RpoC" ] }, { "name": "Rj31_JR3_b", "components": [ "#in2", "Rj31_JR3_b_cassette" ] }, { "name": "Rj31_JR3_b_cassette", "components": [ "RiboJ69", "Rj31", "JR3", "RpoC" ] } ] }, { "collection": "structures", "name": "Rj41_JR4_structure", "inputs": [ { "name": "in1", "part_type": "promoter" }, { "name": "in2", "part_type": "promoter" } ], "outputs": [ "pJR4" ], "devices": [ { "name": "Rj41_JR4_a", "components": [ "#in1", "Rj41_JR4_a_cassette" ] }, { "name": "Rj41_JR4_a_cassette", "components": [ "BydvJ", "Rj41", "JR4", "ECK120029600" ] }, { "name": "Rj41_JR4_b", "components": [ "#in2", "Rj41_JR4_b_cassette" ] }, { "name": "Rj41_JR4_b_cassette", "components": [ "BydvJ", "Rj41", "JR4", "ECK120029600" ] } ] }, { "collection": "structures", "name": "Rj71_JR7_structure", "inputs": [ { "name": "in1", "part_type": "promoter" }, { "name": "in2", "part_type": "promoter" } ], "outputs": [ "pJR7" ], "devices": [ { "name": "Rj71_JR7_a", "components": [ "#in1", "Rj71_JR7_a_cassette" ] }, { "name": "Rj71_JR7_a_cassette", "components": [ "PlmJ", "Rj71", "JR7", "DT36" ] }, { "name": "Rj71_JR7_b", "components": [ "#in2", "Rj71_JR7_b_cassette" ] }, { "name": "Rj71_JR7_b_cassette", "components": [ "PlmJ", "Rj71", "JR7", "DT36" ] } ] }, { "collection": "structures", "name": "Rj72_JR7_structure", "inputs": [ { "name": "in1", "part_type": "promoter" }, { "name": "in2", "part_type": "promoter" } ], "outputs": [ "pJR7" ], "devices": [ { "name": "Rj72_JR7_a", "components": [ "#in1", "Rj72_JR7_a_cassette" ] }, { "name": "Rj72_JR7_a_cassette", "components": [ "PlmJ", "Rj72", "JR7", "DT36" ] }, { "name": "Rj72_JR7_b", "components": [ "#in2", "Rj72_JR7_b_cassette" ] }, { "name": "Rj72_JR7_b_cassette", "components": [ "PlmJ", "Rj72", "JR7", "DT36" ] } ] }, { "collection": "structures", "name": "Rj81_JR8_structure", "inputs": [ { "name": "in1", "part_type": "promoter" }, { "name": "in2", "part_type": "promoter" } ], "outputs": [ "pJR8" ], "devices": [ { "name": "Rj81_JR8_a", "components": [ "#in1", "Rj81_JR8_a_cassette" ] }, { "name": "Rj81_JR8_a_cassette", "components": [ "RiboJ60", "Rj81", "JR8", "DT56" ] }, { "name": "Rj81_JR8_b", "components": [ "#in2", "Rj81_JR8_b_cassette" ] }, { "name": "Rj81_JR8_b_cassette", "components": [ "RiboJ60", "Rj81", "JR8", "DT56" ] } ] }, { "collection": "structures", "name": "Rj91_JR9_structure", "inputs": [ { "name": "in1", "part_type": "promoter" }, { "name": "in2", "part_type": "promoter" } ], "outputs": [ "pJR9" ], "devices": [ { "name": "Rj91_JR9_a", "components": [ "#in1", "Rj91_JR9_a_cassette" ] }, { "name": "Rj91_JR9_a_cassette", "components": [ "RiboJ64", "Rj91", "JR9", "DT86" ] }, { "name": "Rj91_JR9_b", "components": [ "#in2", "Rj91_JR9_b_cassette" ] }, { "name": "Rj91_JR9_b_cassette", "components": [ "RiboJ64", "Rj91", "JR9", "DT86" ] } ] }, { "collection": "structures", "name": "Rj92_JR9_structure", "inputs": [ { "name": "in1", "part_type": "promoter" }, { "name": "in2", "part_type": "promoter" } ], "outputs": [ "pJR9" ], "devices": [ { "name": "Rj92_JR9_a", "components": [ "#in1", "Rj92_JR9_a_cassette" ] }, { "name": "Rj92_JR9_a_cassette", "components": [ "RiboJ64", "Rj92", "JR9", "DT86" ] }, { "name": "Rj92_JR9_b", "components": [ "#in2", "Rj92_JR9_b_cassette" ] }, { "name": "Rj92_JR9_b_cassette", "components": [ "RiboJ64", "Rj92", "JR9", "DT86" ] } ] }, { "collection": "structures", "name": "Rj93_JR9_structure", "inputs": [ { "name": "in1", "part_type": "promoter" }, { "name": "in2", "part_type": "promoter" } ], "outputs": [ "pJR9" ], "devices": [ { "name": "Rj93_JR9_a", "components": [ "#in1", "Rj93_JR9_a_cassette" ] }, { "name": "Rj93_JR9_a_cassette", "components": [ "RiboJ64", "Rj93", "JR9", "DT86" ] }, { "name": "Rj93_JR9_b", "components": [ "#in2", "Rj93_JR9_b_cassette" ] }, { "name": "Rj93_JR9_b_cassette", "components": [ "RiboJ64", "Rj93", "JR9", "DT86" ] } ] }, { "collection": "structures", "name": "Rj111_JR11_structure", "inputs": [ { "name": "in1", "part_type": "promoter" }, { "name": "in2", "part_type": "promoter" } ], "outputs": [ "pJR11" ], "devices": [ { "name": "Rj111_JR11_a", "components": [ "#in1", "Rj111_JR11_a_cassette" ] }, { "name": "Rj111_JR11_a_cassette", "components": [ "SarJ", "Rj111", "JR11", "DT19" ] }, { "name": "Rj111_JR11_b", "components": [ "#in2", "Rj111_JR11_b_cassette" ] }, { "name": "Rj111_JR11_b_cassette", "components": [ "SarJ", "Rj111", "JR11", "DT19" ] } ] }, { "collection": "models", "name": "Rj13_JR1_model", "functions": { "response_function": "Hill_response", "input_composition": "linear_input_composition", "toxicity": "Rj13_JR1_toxicity", "cytometry": "Rj13_JR1_cytometry" }, "parameters": [ { "name": "ymax", "value": 3.779420838, "description": "Maximal transcription" }, { "name": "ymin", "value": 0.035229117, "description": "Minimal transcription" }, { "name": "K", "value": 0.043390621, "description": "Half-maximum" }, { "name": "n", "value": 2.25481011, "description": "Cooperativety" } ] }, { "collection": "models", "name": "Rj14_JR1_model", "functions": { "response_function": "Hill_response", "input_composition": "linear_input_composition", "toxicity": "Rj14_JR1_toxicity", "cytometry": "Rj14_JR1_cytometry" }, "parameters": [ { "name": "ymax", "value": 5.216970779, "description": "Maximal transcription" }, { "name": "ymin", "value": 0.04588324, "description": "Minimal transcription" }, { "name": "K", "value": 0.162932102, "description": "Half-maximum" }, { "name": "n", "value": 2.090738632, "description": "Cooperativety" } ] }, { "collection": "models", "name": "Rj21_JR2_model", "functions": { "response_function": "Hill_response", "input_composition": "linear_input_composition", "toxicity": "Rj21_JR2_toxicity", "cytometry": "Rj21_JR2_cytometry" }, "parameters": [ { "name": "ymax", "value": 2.4406312, "description": "Maximal transcription" }, { "name": "ymin", "value": 0.103434868, "description": "Minimal transcription" }, { "name": "K", "value": 0.217708584, "description": "Half-maximum" }, { "name": "n", "value": 1.5512218, "description": "Cooperativety" } ] }, { "collection": "models", "name": "Rj31_JR3_model", "functions": { "response_function": "Hill_response", "input_composition": "linear_input_composition", "toxicity": "Rj31_JR3_toxicity", "cytometry": "Rj31_JR3_cytometry" }, "parameters": [ { "name": "ymax", "value": 3.268995418, "description": "Maximal transcription" }, { "name": "ymin", "value": 0.031118983, "description": "Minimal transcription" }, { "name": "K", "value": 0.032539108, "description": "Half-maximum" }, { "name": "n", "value": 1.529782691, "description": "Cooperativety" } ] }, { "collection": "models", "name": "Rj41_JR4_model", "functions": { "response_function": "Hill_response", "input_composition": "linear_input_composition", "toxicity": "Rj41_JR4_toxicity", "cytometry": "Rj41_JR4_cytometry" }, "parameters": [ { "name": "ymax", "value": 3.542309059, "description": "Maximal transcription" }, { "name": "ymin", "value": 0.058783804, "description": "Minimal transcription" }, { "name": "K", "value": 0.054176575, "description": "Half-maximum" }, { "name": "n", "value": 1.819793098, "description": "Cooperativety" } ] }, { "collection": "models", "name": "Rj71_JR7_model", "functions": { "response_function": "Hill_response", "input_composition": "linear_input_composition", "toxicity": "Rj71_JR7_toxicity", "cytometry": "Rj71_JR7_cytometry" }, "parameters": [ { "name": "ymax", "value": 1.576950293, "description": "Maximal transcription" }, { "name": "ymin", "value": 0.021819985, "description": "Minimal transcription" }, { "name": "K", "value": 0.02909727, "description": "Half-maximum" }, { "name": "n", "value": 2.548280961, "description": "Cooperativety" } ] }, { "collection": "models", "name": "Rj72_JR7_model", "functions": { "response_function": "Hill_response", "input_composition": "linear_input_composition", "toxicity": "Rj72_JR7_toxicity", "cytometry": "Rj72_JR7_cytometry" }, "parameters": [ { "name": "ymax", "value": 3.75792433, "description": "Maximal transcription" }, { "name": "ymin", "value": 0.026776673, "description": "Minimal transcription" }, { "name": "K", "value": 0.078436787, "description": "Half-maximum" }, { "name": "n", "value": 2.058969157, "description": "Cooperativety" } ] }, { "collection": "models", "name": "Rj81_JR8_model", "functions": { "response_function": "Hill_response", "input_composition": "linear_input_composition", "toxicity": "Rj81_JR8_toxicity", "cytometry": "Rj81_JR8_cytometry" }, "parameters": [ { "name": "ymax", "value": 1.488230227, "description": "Maximal transcription" }, { "name": "ymin", "value": 0.013941128, "description": "Minimal transcription" }, { "name": "K", "value": 0.074900767, "description": "Half-maximum" }, { "name": "n", "value": 1.860140268, "description": "Cooperativety" } ] }, { "collection": "models", "name": "Rj91_JR9_model", "functions": { "response_function": "Hill_response", "input_composition": "linear_input_composition", "toxicity": "Rj91_JR9_toxicity", "cytometry": "Rj91_JR9_cytometry" }, "parameters": [ { "name": "ymax", "value": 1.896985729, "description": "Maximal transcription" }, { "name": "ymin", "value": 0.031406958, "description": "Minimal transcription" }, { "name": "K", "value": 0.481807912, "description": "Half-maximum" }, { "name": "n", "value": 2.659059916, "description": "Cooperativety" } ] }, { "collection": "models", "name": "Rj92_JR9_model", "functions": { "response_function": "Hill_response", "input_composition": "linear_input_composition", "toxicity": "Rj92_JR9_toxicity", "cytometry": "Rj92_JR9_cytometry" }, "parameters": [ { "name": "ymax", "value": 1.819126514, "description": "Maximal transcription" }, { "name": "ymin", "value": 0.024877707, "description": "Minimal transcription" }, { "name": "K", "value": 0.092760168, "description": "Half-maximum" }, { "name": "n", "value": 2.18283262, "description": "Cooperativety" } ] }, { "collection": "models", "name": "Rj93_JR9_model", "functions": { "response_function": "Hill_response", "input_composition": "linear_input_composition", "toxicity": "Rj93_JR9_toxicity", "cytometry": "Rj93_JR9_cytometry" }, "parameters": [ { "name": "ymax", "value": 1.023211547, "description": "Maximal transcription" }, { "name": "ymin", "value": 0.021905529, "description": "Minimal transcription" }, { "name": "K", "value": 0.032525222, "description": "Half-maximum" }, { "name": "n", "value": 2.521017808, "description": "Cooperativety" } ] }, { "collection": "models", "name": "Rj111_JR11_model", "functions": { "response_function": "Hill_response", "input_composition": "linear_input_composition", "toxicity": "Rj111_JR11_toxicity", "cytometry": "Rj111_JR11_cytometry" }, "parameters": [ { "name": "ymax", "value": 2.636669224, "description": "Maximal transcription" }, { "name": "ymin", "value": 0.018283171, "description": "Minimal transcription" }, { "name": "K", "value": 0.103894911, "description": "Half-maximum" }, { "name": "n", "value": 2.04086841, "description": "Cooperativety" } ] }, { "collection": "functions", "name": "Hill_response", "equation": "ymin + (ymax - ymin) / (1.0 + (x / K)^n)", "variables": [ { "name": "x", "map": "#//model/functions/input_composition" } ], "parameters": [ { "name": "ymax", "map": "#//model/parameters/ymax" }, { "name": "ymin", "map": "#//model/parameters/ymin" }, { "name": "K", "map": "#//model/parameters/K" }, { "name": "n", "map": "#//model/parameters/n" } ] }, { "collection": "functions", "name": "linear_input_composition", "equation": "x1 + x2", "variables": [ { "name": "x1", "map": "#//structure/inputs/in1/model/functions/response_function" }, { "name": "x2", "map": "#//structure/inputs/in2/model/functions/response_function" } ] }, { "collection": "circuit_rules", "rules": { "function": "AND", "rules": [ { "function": "AND", "rules": [ "STARTSWITH attB2", "JR3_1 NEXTTO L1", "attB7 BEFORE attB5", "Rj13_JR1 BEFORE attB5", "Rj14_JR1 BEFORE attB5", "Rj21_JR2 BEFORE attB5", "Rj31_JR3 BEFORE attB5", "Rj41_JR4 BEFORE attB5", "Rj71_JR7 BEFORE attB5", "Rj72_JR7 BEFORE attB5", "Rj81_JR8 BEFORE attB5", "Rj91_JR9 BEFORE attB5", "Rj92_JR9 BEFORE attB5", "Rj93_JR9 BEFORE attB5", "Rj111_JR11 BEFORE attB5", "Rj51_JR5 BEFORE attB5", "Rj61_JR6 BEFORE attB5", "Rj121_JR12 BEFORE attB5", "R11_N15 BEFORE attB5", "Rj101_JR10 BEFORE attB5", "OC6_out AFTER attB5", "pC_HSL_out AFTER attB5", "DAPG_out AFTER attB5", "OHC12_out AFTER attB5", "ALL_FORWARD" ] }, { "function": "OR", "rules": [ { "function": "AND", "rules": [ "P1_PhlF_a AFTER L2", "P1_PhlF_a BEFORE L3", "P1_PhlF_b AFTER L3" ] }, { "function": "AND", "rules": [ "P1_PhlF_a AFTER L2", "P1_PhlF_a BEFORE L3", "P1_PhlF_b BEFORE L2" ] }, { "function": "AND", "rules": [ "P1_PhlF_b AFTER L2", "P1_PhlF_b BEFORE L3", "P1_PhlF_a BEFORE L2" ] }, { "function": "AND", "rules": [ "P1_PhlF_b AFTER L2", "P1_PhlF_b BEFORE L3", "P1_PhlF_a BEFORE L2" ] } ] }, { "function": "OR", "rules": [ { "function": "AND", "rules": [ "Q1_QacR_a AFTER L2", "Q1_QacR_a BEFORE L3", "Q1_QacR_b AFTER L3" ] }, { "function": "AND", "rules": [ "Q1_QacR_a AFTER L2", "Q1_QacR_a BEFORE L3", "Q1_QacR_b BEFORE L2" ] }, { "function": "AND", "rules": [ "Q1_QacR_b AFTER L2", "Q1_QacR_b BEFORE L3", "Q1_QacR_a BEFORE L2" ] }, { "function": "AND", "rules": [ "Q1_QacR_b AFTER L2", "Q1_QacR_b BEFORE L3", "Q1_QacR_a BEFORE L2" ] } ] }, { "function": "OR", "rules": [ { "function": "AND", "rules": [ "A1_AmtR_a AFTER L2", "A1_AmtR_a BEFORE L3", "A1_AmtR_b AFTER L3" ] }, { "function": "AND", "rules": [ "A1_AmtR_a AFTER L2", "A1_AmtR_a BEFORE L3", "A1_AmtR_b BEFORE L2" ] }, { "function": "AND", "rules": [ "A1_AmtR_b AFTER L2", "A1_AmtR_b BEFORE L3", "A1_AmtR_a BEFORE L2" ] }, { "function": "AND", "rules": [ "A1_AmtR_b AFTER L2", "A1_AmtR_b BEFORE L3", "A1_AmtR_a BEFORE L2" ] } ] }, { "function": "OR", "rules": [ { "function": "AND", "rules": [ "B3_BM3R1_a AFTER L2", "B3_BM3R1_a BEFORE L3", "B3_BM3R1_b AFTER L3" ] }, { "function": "AND", "rules": [ "B3_BM3R1_a AFTER L2", "B3_BM3R1_a BEFORE L3", "B3_BM3R1_b BEFORE L2" ] }, { "function": "AND", "rules": [ "B3_BM3R1_b AFTER L2", "B3_BM3R1_b BEFORE L3", "B3_BM3R1_a BEFORE L2" ] }, { "function": "AND", "rules": [ "B3_BM3R1_b AFTER L2", "B3_BM3R1_b BEFORE L3", "B3_BM3R1_a BEFORE L2" ] } ] }, { "function": "OR", "rules": [ { "function": "AND", "rules": [ "F2_AmeRs_a AFTER L2", "F2_AmeRs_a BEFORE L3", "F2_AmeRs_b AFTER L3" ] }, { "function": "AND", "rules": [ "F2_AmeRs_a AFTER L2", "F2_AmeRs_a BEFORE L3", "F2_AmeRs_b BEFORE L2" ] }, { "function": "AND", "rules": [ "F2_AmeRs_b AFTER L2", "F2_AmeRs_b BEFORE L3", "F2_AmeRs_a BEFORE L2" ] }, { "function": "AND", "rules": [ "F2_AmeRs_b AFTER L2", "F2_AmeRs_b BEFORE L3", "F2_AmeRs_a BEFORE L2" ] } ] }, { "function": "OR", "rules": [ { "function": "AND", "rules": [ "E1_BetI_a AFTER L2", "E1_BetI_a BEFORE L3", "E1_BetI_b AFTER L3" ] }, { "function": "AND", "rules": [ "E1_BetI_a AFTER L2", "E1_BetI_a BEFORE L3", "E1_BetI_b BEFORE L2" ] }, { "function": "AND", "rules": [ "E1_BetI_b AFTER L2", "E1_BetI_b BEFORE L3", "E1_BetI_a BEFORE L2" ] }, { "function": "AND", "rules": [ "E1_BetI_b AFTER L2", "E1_BetI_b BEFORE L3", "E1_BetI_a BEFORE L2" ] } ] } ] } }, { "collection": "device_rules", "rules": { "function": "AND", "rules": [ "ALL_FORWARD" ] } }, { "collection": "parts", "type": "promoter", "name": "pJR1", "dnasequence": "TTCTTTCAGGCCGGGtaacaccgtgcgtgttgactattttacctctggcggtgataatagct" }, { "collection": "parts", "type": "cds", "name": "JR1", "dnasequence": "atgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcTGA" }, { "collection": "parts", "type": "rbs", "name": "Rj13", "dnasequence": "AACATCACACGTTAGCCTCTAC" }, { "collection": "parts", "type": "rbs", "name": "Rj14", "dnasequence": "AACATCCCTCGTTAGCCTCTTC" }, { "collection": "parts", "type": "ribozyme", "name": "RiboJ", "dnasequence": "AGCTGTCACCGGATGTGCTTTCCGGTCTGATGAGTCCGTGAGGACGAAACAGCCTCTACAAATAATTTTGTTTAA" }, { "collection": "parts", "type": "ribozyme", "name": "RiboJ54", "dnasequence": "aggggtcagttgatgtgctttcaactctgatgagtcagtgatgacgaaaccccctctacaaataattttgtttaa" }, { "collection": "parts", "type": "terminator", "name": "Ret0", "dnasequence": "GCAGACAAAAAAAATGGCGCACAATGTGCGCCATTTTTCACTTCACAGGTACTATTGTTTTGAATTGAAAAGGGCGCTTCGGCGCCCTTTTTGCATTTGTTGACGGCATATATTTGTATATCGAAGCGCCCTGATGGGCGCTTTTTTTATTTAATCGATAACCAGA" }, { "collection": "parts", "type": "promoter", "name": "pJR2", "dnasequence": "GATGAGGCGGTCAGGaataaacttgactaaagattcctttagtagataatttaagtgttctttaatttc" }, { "collection": "parts", "type": "cds", "name": "JR2", "dnasequence": "ATGGGAGAGCGTATCCGTGCGCGCCGTAAAAAATTAAAAATCCGCCAGGCAGCGTTAGGCAAGATGGTCGGTGTTTCGAATGTAGCGATCTCTCAGTGGGAGCGTAGCGAAACTGAACCGAATGGAGAAAATTTATTGGCTTTATCTAAGGCACTGCAGTGTAGTCCGGACTACTTGTTGAAAGGTGATCTGAGTCAAACAAACGTCGCGTACCATTCTCGCCATGAACCACGTGGCTCTTATCCTCTGATTAGCTGGGTGTCTGCGGGGCAGTGGATGGAGGCGGTCGAACCGTATCACAAGCGTGCAATCGAGAATTGGCATGATACGACTGTAGACTGTAGCGAGGATTCGTTCTGGTTGGATGTTCAAGGGGATAGCATGACCGCACCCGCCGGATTAAGCATCCCCGAAGGCATGATTATTTTGGTCGATCCGGAAGTGGAGCCTCGCAACGGCAAACTTGTCGTGGCTAAGTTGGAAGGCGAGAATGAGGCGACGTTTAAGAAACTTGTCATGGATGCCGGACGTAAGTTTTTGAAACCCTTAAATCCGCAATATCCGATGATCGAGATCAATGGAAACTGCAAAATTATCGGTGTAGTAGTTGATGCCAAGCTGGCTAACTTACCGTAG" }, { "collection": "parts", "type": "rbs", "name": "Rj21", "dnasequence": "ACTTTTAGCTAAAGGAATGATTG" }, { "collection": "parts", "type": "rbs", "name": "Rj11", "dnasequence": "CAGGTTTTGAGGCGTGTATTGGCAGGCTACTAG" }, { "collection": "parts", "type": "rbs", "name": "Rj12", "dnasequence": "CAGGTTTTGAGCCGTGTATCGTCAGGCTACTAG" }, { "collection": "parts", "type": "rbs", "name": "Rj22", "dnasequence": "GTTCGGGATTAGTCGTCTTTGGAA" }, { "collection": "parts", "type": "rbs", "name": "Rj42", "dnasequence": "TTCATCACTTCGCCGTTGATCG" }, { "collection": "parts", "type": "ribozyme", "name": "RiboJ71", "dnasequence": "AGTTGTCAGCCACTGTGCTTGTGGCTCTGATGAGACGGTGACGTCGAAACAACCTCTACAAATAATTTTGTTTAA" }, { "collection": "parts", "type": "terminator", "name": "IlvGEDA", "dnasequence": "TAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACA" }, { "collection": "parts", "type": "promoter", "name": "pJR3", "dnasequence": "GCCACAAGCTCGAAAagttcatgaaacgtgaacttgacagctagctcagtcctaggtataaagttcatgaaacgtgaact" }, { "collection": "parts", "type": "cds", "name": "JR3", "dnasequence": "ATGAAGACTGACACATCAAATCGCTTAAAGCAGATCATGGCTGAGCGCAACCTTAAACAAGTGGATATCCTGAATCTTAGCATCCCTTTTCAAAAAAAATTCGGGATCAAATTGAGCAAGTCCACCCTTTCGCAATACGTCAACTCGGTACAATCCCCAGACCAGAACCGTATTTACCTGTTAGCCAAGACCTTGGGGGTAAGTGAAGCATGGTTGATGGGATTCGACGTTCCAATGGTGGAATCCTCGAAGATTGAGAATGATTCAGAGAACATTGAGGAGACGATCACCGTCATGAAGAAGCTGGAAGAGCCGCGTCAGAAAGTGGTCTTGGATACAGCAAAAATTCAGTTAAAGGAGCAGGATGAACAGAATAAGGTAAAGCAGATCGAGGACTATCGCTTATCCGACGAATATTTGGAGGAGCAGATCAGCAAGGCGTCGGCTTACGGAGGGGGGCAACTTAACGATAATGATAAGGAATTTTTCAAACGTCTGCTGAAAAACACCCTTAAGGAGAAAATTGACAAAGGCGATCTTTGA" }, { "collection": "parts", "type": "rbs", "name": "Rj31", "dnasequence": "GCTGGTACTGCTCGAGTATCTT" }, { "collection": "parts", "type": "ribozyme", "name": "RiboJ69", "dnasequence": "AGCTGTCAAGGCGTGTGCTTCGCCTTCTGATGAGCCCGTGAGGGCGAAACAGCCTCTACAAATAATTTTGTTTAA" }, { "collection": "parts", "type": "terminator", "name": "RpoC", "dnasequence": "GTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCA" }, { "collection": "parts", "type": "promoter", "name": "pJR4", "dnasequence": "GAGAGCTAACTTTATaacttgacaaattggatagttttaggtataataacttgacaaattggatagtt" }, { "collection": "parts", "type": "cds", "name": "JR4", "dnasequence": "ATGAAGAAGATTCGCCTTCCCGAAATGATTGACTACTTCCGTAAGGAAAACGGCTGGACTATGAAAGAGTTTGGTGAAAAATTAGGGAAGAGTGAGAGCGCTATTAGCAAGTGGATCAAGGGGGTACGCTCCCCTATGGTAGAGGATTTTGACAAGATGGTGAATTTATTTAACACTGACCCAGAAACCCTGATGTATGGTGCGTCAGATTTATCTACGACGTTGAGCGAAATCAATAAGATTTCGAGCCAACTTGAGGAACCGCGCCAGAAGGTTGTACTGAATACGGCAAATAATCAGCTTGACGAACAAAATCAGGAAAAGAAAAAGGAATCTAAAGTCATCCCTATTAACAAAATCCCAGATGACTTACCACCCTACATTAGTCGCAAAATTCTGGAAAATTTTGTAATGCCGACTAACACTATGGAATACGAAGCTGATGAAGATATGGTGGACGTACCCATCCTTGGGCGCATCGCGGCGGGCCTTCCGCTTGACGCGGTAGAAAACTTTGATGGAACGCGCCCAGTCCCTGCACATTTCCTTTCGAGTGCCCGTGATTACTATTGGCTTATGGTGGACGGCCATTCTATGGAACCTAAGATTCCGTACGGAGCATACGTCTTGATCGAAGCCGTCCCCGATGTGAGCGATGGGACCATTGGTGCCGTCTTATTCCATGATGATTGTCAGGCGACGCTGAAAAAGGTATATCACGAGATCGACTGTTTGCGCTTAGTCTCAATTAACAAAGAATTTAAGGACCAATTCGCCACGCAAGATAATCCAGCTGCAGTGATTGGTCAAGCAGTTAAGGTTGAAATCGACTTGTAA" }, { "collection": "parts", "type": "rbs", "name": "Rj41", "dnasequence": "TATTTTTAATAAATAGGGTT" }, { "collection": "parts", "type": "ribozyme", "name": "BydvJ", "dnasequence": "agggtgtctcaaggtgcgtaccttgactgatgagtccgaaaggacgaaacacccctctacaaataattttgtttaa" }, { "collection": "parts", "type": "terminator", "name": "ECK120029600", "dnasequence": "TTCAGCCAAAAAACTTAAGACCGCCGGTCTTGTCCACTACCTTGCAGTAATGCGGTGGACAGGATCGGCGGTTTTCTTTTCTCTTCTCAA" }, { "collection": "parts", "type": "ribozyme", "name": "ElvJ", "dnasequence": "agccccatagggtggtgtgtaccacccctgatgagtccaaaaggacgaaatggggcctctacaaataattttgtttaa" }, { "collection": "parts", "type": "promoter", "name": "pJR5", "dnasequence": "AGTCTGGGAGGTACTtttcttgacaaaccacgaaaagtggactataattaag" }, { "collection": "parts", "type": "cds", "name": "JR5", "dnasequence": "ATGGTTATCGAACAAATCAACAAGTACGTTGGAAGCAAGATTAAGGATTATCGTAAATCATTTGGACTTTCCCAGGAAGAATTGGCTAAAAAAATTGGAGTCGGCAAAACCACTATCTCTAATTATGAAGTAGGGATCCGCTCACCAAAAAAGCCTCAGCTTATTAAATTATCCGAGGTGTTCGATGTAGCAATTGATGATTTTTTTCCTCAGACTGATAGTACACGCATGAACGTGTCTAGCATCCTGTCCGAAATCAACAAGATTTCCTCCCAGCTTGAAGAACCGCGCCAAAAGATCGTGCTTAATACCGCTACCAACCAACTTGATGAGCAGAATCAAGAAAAGAAAAAGGAAAGTAAGGTCATTCCTATCAATAAGATCCCTGACGATCTTCCACCTTACATTTCCCGCAAAATTTTAGAGAATTTTATTATGCCTTCCAATACGATGGAATATGAACCCGACGAGGACATGGTAGACGTTCCGATTTTGGGGCGTATTGCCGCCGGGTTACCACTGGATGCGGTCGAAAACTTCGATGGAACCCGTCCCGTACCAGCCCATTTCTTATCAAGTGCCCGTGATTACTACTGGTTAATGGTAGACGGTCACAGTATGGAACCGAAGATTCCTTATGGAGCCTACGTGCTGATTGAGGCGGTTCCCGATGTGTCAGACGGTACTATTGGTGCTGTACTTTTCCAAGATGACTGCCAGGCCACGTTAAAGAAGGTATATCACGAGATCGACTGTTTACGCCTGGTGTCCATTAACAAAGAGTTTAAGGATCAGTTTGCCACGCAAGACAATCCTGCTGCTGTTATCGGGCAGGCAGTGAAAGTAGAAATCGATTTGTAA" }, { "collection": "parts", "type": "rbs", "name": "Rj51", "dnasequence": "AGAATATTATTGAAGATAATAGGA" }, { "collection": "parts", "type": "promoter", "name": "pJR10", "dnasequence": "GGTGCAGTTTTGTGCgattgcaaaatttgcatttacttttattatcttttttgataaaaact" }, { "collection": "parts", "type": "cds", "name": "JR10", "dnasequence": "atgaaggaaaataagaggatgatagaaatagatattggcccggtattaaagcgcatccgttatgagcgagggctaacgctacaaaagctgtcacggcttacggatgacaaagtgctaccaagcaacatttcccgcatcgagagtgcgggcgctggcgctacgcttaaaaccttgaccaccttggcgaatgcgctggggacttctccttctgacattcttcgtgaagctgagggcggtgataaggtgatcaccaagccgcagcaagtcctttatgtacctgttttgtcttgggttcaggctggcacctggactgaatcaccagagcagcctgccgatggtgactatgatgaatgggtagaggcaccaagaggcgcatccaggaaggctttcggccttcgagttcaaggggatagtatgcaagcccctatcggcaagtcgttcccggaaggatgctgcatagttgttgacccaaccaaacaagcagataaccgctcgtttgttgttgctcgcctggcagacacaggagagcacacgttcaagcagctgatcattgacgggccgcaccaatatctaaagccgctaaacccaagttaccgaaccatagaagtgaacagcgaagtacacgtctgcggggtcgttttagcatggggtgaagggtacacggtgaacggtatttaa" }, { "collection": "parts", "type": "rbs", "name": "Rj101", "dnasequence": "CGGATCTAGTAGGTACAGTGCAATC" }, { "collection": "parts", "type": "ribozyme", "name": "SarJ", "dnasequence": "gactgtcgccggatgtgtatccgacctgacgatggcccaaaagggccgaaacagtcctctacaaataattttgtttaa" }, { "collection": "parts", "type": "terminator", "name": "DT19", "dnasequence": "TTCAGCCAAAAAACTTAAGACCGCCGGTCTTGTCCACTACCTTGCAGTAATGCGGTGGACAGGATCGGCGGTTTTCTTTTCTCTTCTCAACTCGGTACCAAAGACGAACAATAAGACGCTGAAAAGCGTCTTTTTTCGTTTTGGTCC" }, { "collection": "parts", "type": "promoter", "name": "pJR6", "dnasequence": "TTGTTACCCATACTGtttcgataaaacctatttactatctctcaattgggagatatattttggctaaaccca" }, { "collection": "parts", "type": "cds", "name": "JR6", "dnasequence": "atgagaatagattctttaggatggagcaacgtggatgtacttgaccgcatctgcgaggcgtacggattttcgcagaaaattcaattagctaaccacttcgatatcgcgtccagctcattgtcaaacaggtacacccgaggcgctatctcctatgactttgcggctcactgtgcccttgaaacaggtgctaatctccagtggttacttaccggagaaggagaagcatttgtaaataacagagaatcgagcgacgcaaaaaggattgagggattcacattaagtgaagaaatcctcaaatccgataaacaattgagtgttgatgcccaatttttcacaaaaccgctcacagatgggatggctatccgttccgaggggaaaatttattttgtggacaagcaagcatcattgtctgacggcttatggctagtcgacattgagggagcaataagcattcgagagttaacaaaactaccgggtagaaaactacatgttgcaggcgggaaggttcctttcgaatgcggcattgatgatattaaaacgttggggcgtgtggtgggtgtatacagcgaggttaactaa" }, { "collection": "parts", "type": "rbs", "name": "Rj61", "dnasequence": "AAGAGGCGACGTAACATCCTGGAGGCAAGTAATAACT" }, { "collection": "parts", "type": "ribozyme", "name": "RiboJ10", "dnasequence": "agcgctcaacgggtgtgcttcccgttctgatgagtccgtgaggacgaaagcgcctctacaaataattttgtttaa" }, { "collection": "parts", "type": "terminator", "name": "DT83", "dnasequence": "AGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTAGGCAGCATGCTGCCAGGTGATCCCCCTGGCCACCTCTTTT" }, { "collection": "parts", "type": "promoter", "name": "pJR7", "dnasequence": "ATTGTCAGAAATGGCacttgcaaaaactttcagttcaaccataatacgtactgaaagtacgaaaaagg" }, { "collection": "parts", "type": "cds", "name": "JR7", "dnasequence": "atggttcagaatgaaaaagtgcgcaaagaattcgcccagcggctagcgcaagcctgtaaagaagctggtcttgatgaacatggtaggggaatggctatagcccgtgccctttctctttcgtccaaaggcgttagcaaatggtttaatgctgagtctttaccgcgtcaggaaaaaatgaatgcgcttgcgaaatttctaaacgttgatgttgtttggcttcagcacggcacttcgttaaatggagcgaatgatgaagatactctttcatttgttggcaaattaaaaaaagggttagtgcgcgtggttggtgaggcaattcttggtgttgatggtgccatcgagatgaccgaagaacgcgatgggtggctcaaaatttatagcgatgatccagatgcctttggtctgcgtgtgaaaggagacagcatgtggcccagaataaaatcaggagaatatgtactcattgagcctaacaccaaagtattcccgggtgatgaggtgtttgtcagaaccgttgaaggacacaacatgattaaggttcttggctatgacagagatggagaataccaatttacaagcattaaccaggatcacaggcctataacgttgccttatcatcaagtagcaaaggtggagtatgtagctggtattctgaagcaatctcgccatctggatgacatcgaggcaagggagtggctgaaaagttcgtga" }, { "collection": "parts", "type": "rbs", "name": "Rj71", "dnasequence": "GCACTACGGAATTAAGCGCTCAA" }, { "collection": "parts", "type": "rbs", "name": "Rj72", "dnasequence": "GCACTACGGCATTAAGCGCTCAA" }, { "collection": "parts", "type": "rbs", "name": "Rj73", "dnasequence": "ACTTCGGCGCACCGGCTAGGACTAC" }, { "collection": "parts", "type": "ribozyme", "name": "PlmJ", "dnasequence": "agtcataagtctgggctaagcccactgatgagtcgctgaaatgcgacgaaacttatgacctctacaaataattttgtttaa" }, { "collection": "parts", "type": "terminator", "name": "DT36", "dnasequence": "GATCTAACTAAAAAGGCCGCTCTGCGGCCTTTTTTCTTTTCACTGTAACAACGGAAACCGGCCATTGCGCCGGTTTTTTTTGGCCT" }, { "collection": "parts", "type": "promoter", "name": "pJR12", "dnasequence": "TGTTAATGTAATCTGcttgacagccacctcagtcctaggtactgtgctagcttgacagccacc" }, { "collection": "parts", "type": "cds", "name": "JR12", "dnasequence": "atgagcggcaaaatccaacacaaagcagtggttccggcccctagccgaataccactcactctcagcgagatcgaagatcttcgcaggaaggggtttaatcagaccgaaatcgcagagctgtatggcgtgactcgacaagcggtgtcgtggcacaagaaaacctacggaggacggttgaccaccaggcagatcgtccagcagaactggccgtgggacacgcggaaacctcacgataagtcgaaggcttttcagaggctccgggatcacggcgagtacatgcgagtgggtagcttccgcacgatgtccgaagataagaagaagcgcctattgtcgtggtggaagatgctccgcgacgacgacttagtgctcgagttcgacccatccatcgagccctacgaaggtatggccggcggcgggttcaggtacgtcccccgaggcatcgaggatgacgatctcctgatccgggtgaacgagcacaccaacctcaccgccgagggtgaactcctctggtcgtggccagacgacatcgaggagcttctctcggagccctaa" }, { "collection": "parts", "type": "rbs", "name": "Rj121", "dnasequence": "CAATTATAAGGCCTGACGCGT" }, { "collection": "parts", "type": "ribozyme", "name": "RiboJ60", "dnasequence": "ctgaagtcgtcaagtgctgtgcttgcacttctgatgaggcagtgatgccgaaacgacctctacaaataattttgtttaa" }, { "collection": "parts", "type": "ribozyme", "name": "RiboJ53", "dnasequence": "AGCGGTCAACGCATGTGCTTTGCGTTCTGATGAGACAGTGATGTCGAAACCGCCTCTACAAATAATTTTGTTTAA" }, { "collection": "parts", "type": "ribozyme", "name": "SccJ", "dnasequence": "AGATGCTGTAGTGGGATGTGTGTCTCACCTGAAGAGTACAAAAGTCCGAAACGGTATCCTCTACAAATAATTTTGTTTAA" }, { "collection": "parts", "type": "terminator", "name": "L3S2P11", "dnasequence": "ctcggtaccaaattccagaaaagagacgctttcgagcgtcttttttcgttttggtcc" }, { "collection": "parts", "type": "promoter", "name": "pN15", "dnasequence": "ACGTTAAAACCCACTaaattatacctagctttaattttcacttattgattataataatccc" }, { "collection": "parts", "type": "cds", "name": "N15", "dnasequence": "atgattaagggtatgaaaacacgaggcgaacgactgaaagcacgccgtttagagctgaaactgacgctgaagcaagtggcagaagctgtaggaatctctcttccgggcgtccaaaacttagaacgtggcgacgtgatgccgtcgctggagatcgggctatcgctggcaaaatgcctgcgtaagcccgtgcaatggatactgtatggcactgaatctgatccagaccgcgttcctgttattggtacgacagaaaccggaccggatagagactggcagcctggagaacctgccaacacagagcgattcctgccgttcgtgagtcaacggaacaccgtttacgcactgacggtcgggaaccagattcagcgaaactaccagccgggagacgttatcctggtcgattcttcacttacgcttgtaccgggtgaggatgtactggtttgtgataataacggcgagatcacaattcaacgattagcccgttatgacgagcagcattactaccttgatagtgctaactctcaacgggttatccatgataaaagtgatcttcaatttgtgcaccaagtagtcggtacgatcaaatcgttcatggttgagggtagatga" }, { "collection": "parts", "type": "rbs", "name": "R11", "dnasequence": "CAAGGGAGCAGGAAGAGACTTCGTAAACTGCG" }, { "collection": "parts", "type": "ribozyme", "name": "ScmJ", "dnasequence": "agcgctgtctgtacttgtatcagtacactgacgagtccctaaaggacgaaacaccgcctctacaaataattttgtttaa" }, { "collection": "parts", "type": "terminator", "name": "DT42", "dnasequence": "AGTTAACCAAAAAGGGGGGATTTTATCTCCCCTTTAATTTTTCCTCGCAGATAGCAAAAAAGCGCCTTTAGGGCGCTTTTTTACATTGGTGG" }, { "collection": "parts", "type": "promoter", "name": "pJR8", "dnasequence": "AACTACTACGCAAAAttcaacttctccgaaacgtctattgacataattactccgattgcgtaatttcttg" }, { "collection": "parts", "type": "promoter", "name": "pJR9", "dnasequence": "ATGATTGGCGACTCGgtttagatctcattgacatggtgtttagatctcaagtactgttagtttagatgtacaattttgttagtttagat" }, { "collection": "parts", "type": "promoter", "name": "pJR11", "dnasequence": "TCCTATAAGGACACGcatttgacagtaacctattggtgacttatattcccgtcaaaaggttgtgtattggtgacctt" }, { "collection": "parts", "type": "cds", "name": "JR8", "dnasequence": "atgtcaatagacgtttcggagaagttgaagctaatccgtgaatctgaaaggttaaaccgtaaagaattcagtgaattaactggtgtagcctacagctcactttcgagctatgagagccggtcaaaaaacgctggagttgaagccataatgaaggtcttacaacatcctagatttactaaatatactttgtggttcatgactgatcaggtagctccagaagccgggcaaattgcgcccgctctcgcacactttgggcaaaacgaaacaacgtcgccccactccggtcaaaagactggttaa" }, { "collection": "parts", "type": "cds", "name": "JR9", "dnasequence": "atgtcaaacacgataagcgagaagatagtcttaatgcgaaaatcagagtatttgagcagacaacaacttgctgatttaacaggggttccgtatggcacgctgagttactatgaaagtggtcgttcaacacctccaacagatgtcatgatgaacatcctgcagaccccacaattcaccaaatacactttatggttcatgaccaatcagatcgctcctgagtccgggcaaattgcgcccgctctcgcacactttgggcaaaacgaaacaacgtcgccccactccggtcaaaagactggttaa" }, { "collection": "parts", "type": "cds", "name": "JR11", "dnasequence": "atgcagacattcgaaaaactgaaagcgattaggaaagcagaaggcttaacacaggcgaaattcagcgaaattagcgggatagctctaggaacagtcaaaaattacgaaagtgggcataaagaccctggtctgagcatcgttatgcgagtcacaaatacgcctttatttaaaaaatatacgctctggttaatgactggtgatacgtcaccacaagctggtcagatcgcgccggctctcgcacacattgggcaaaaaccaacagaatcagaccactccgaaaaacagactggttaa" }, { "collection": "parts", "type": "promoter", "name": "pTP901", "dnasequence": "AAAAAAATTCATATTTCGTGAACTTTTTTGTTGACAAAGATAAAAACACATGATATACTAATTTCATAAAGTTCATGAAACGTGAACTGAAAGA" }, { "collection": "parts", "type": "promoter", "name": "pJR3_2", "dnasequence": "GAAAGAAGTTCATGAAACGTGAACTACAGTTCATGAAACGTGAACTTGACAGCTAGCTCAGTCCTAGGTATAAAGTTCATGAAACGTGAACTGTTAGTTCATGAAACGTGAACTAGCT" }, { "collection": "parts", "type": "promoter", "name": "pr1t", "dnasequence": "TTAACTTGACAAATTGGATAGTTAAGGTTATAATT" }, { "collection": "parts", "type": "promoter", "name": "pP2", "dnasequence": "TTGACATGGTGTTTAGATCTCAATAGTATTTAGTTTAGATGTA" }, { "collection": "parts", "type": "promoter", "name": "pL5", "dnasequence": "CTTGACAGCCACCCGGCCAGTAGTGCATTCTTGT" }, { "collection": "parts", "type": "rbs", "name": "Rj81", "dnasequence": "TTATCTTAGGTACAGGGCACATAA" }, { "collection": "parts", "type": "rbs", "name": "Rj82", "dnasequence": "TGGCGGCAACACAAATACTTG" }, { "collection": "parts", "type": "rbs", "name": "Rj91", "dnasequence": "CAGGTTATAAGGCCTGCAGCGT" }, { "collection": "parts", "type": "rbs", "name": "Rj92", "dnasequence": "TTTTTCTCCACTACGATGGTAAT" }, { "collection": "parts", "type": "rbs", "name": "Rj93", "dnasequence": "TTTGTCCCTTCCACTAGGCTGGTAAT" }, { "collection": "parts", "type": "rbs", "name": "Rj94", "dnasequence": "AAGCACGCGCGTATTTGTCCTT" }, { "collection": "parts", "type": "rbs", "name": "Rj111", "dnasequence": "TGCCACTTACATTAGCGTTTTTGA" }, { "collection": "parts", "type": "rbs", "name": "Rj112", "dnasequence": "AATTATCTAATTCCTTTCTGAATCCGCC" }, { "collection": "parts", "type": "rbs", "name": "Rj122", "dnasequence": "ATCGCACAATAACTCATTTTAA" }, { "collection": "parts", "type": "rbs", "name": "CHRBS3t", "dnasequence": "TGGTTCCAAAGCCAGATACTAAGGAGGTCCCA" }, { "collection": "parts", "type": "rbs", "name": "CHRBS3c", "dnasequence": "CGGTTCCAAAGCCAGATACTAAGGAGGTCCCAC" }, { "collection": "parts", "type": "rbs", "name": "RBS640", "dnasequence": "AAGCGATACTTAGGGTAACAGTA" }, { "collection": "parts", "type": "rbs", "name": "B0034", "dnasequence": "TACTAGAGAAAGAGGAGAAATACTAG" }, { "collection": "parts", "type": "rbs", "name": "B0032", "dnasequence": "TCACACAGGAAAGTACTAG" }, { "collection": "parts", "type": "rbs", "name": "B0064", "dnasequence": "TACTAGAGAAAGAGGGGAAATACTAG" }, { "collection": "parts", "type": "ribozyme", "name": "RiboJ64", "dnasequence": "aggagtcaattaatgtgcttttaattctgatgagacggtgacgtcgaaactccctctacaaataattttgtttaa" }, { "collection": "parts", "type": "terminator", "name": "DT183", "dnasequence": "AGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTAGGCAGCATGCTGCCAGGTGATCCCCCTGGCCACCTCTTTT" }, { "collection": "parts", "type": "terminator", "name": "DT65", "dnasequence": "TGCTCGTACCAGGCCCCTGCAATTTCAACAGGGGCCTTTTTTTATCCAATTCCATCGGGTCCGAATTTTCGGACCTTTTCTCCGC" }, { "collection": "parts", "type": "terminator", "name": "DT56", "dnasequence": "TACCACCGTCAAAAAAAACGGCGCTTTTTAGCGCCGTTTTTATTTTTCAACCTTCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTC" }, { "collection": "parts", "type": "terminator", "name": "DT86", "dnasequence": "TAATCATTCTTAGCGTGACCGGGAAGTCGGTCACGCTACCTCTTCTGAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCT" }, { "collection": "parts", "type": "terminator", "name": "M13", "dnasequence": "GGCTCCTTTTGGAGCCTTTTTTTTT" }, { "collection": "parts", "type": "terminator", "name": "B0015", "dnasequence": "CCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCTACTAGAGTCACACTGGCTCACCTTCGGGTGGGCCTTTCTGCGTTTATA" }, { "collection": "parts", "type": "terminator", "name": "ECK120010850", "dnasequence": "AGTTAACCAAAAAGGGGGGATTTTATCTCCCCTTTAATTTTTCCT" }, { "collection": "parts", "type": "terminator", "name": "ECK120017009", "dnasequence": "GATCTAACTAAAAAGGCCGCTCTGCGGCCTTTTTTCTTTTCACT" }, { "collection": "parts", "type": "terminator", "name": "ECK120033737", "dnasequence": "GGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGG" }, { "collection": "parts", "type": "terminator", "name": "B1006", "dnasequence": "TAAAAAAAAAAACCCCGCCCCTGACAGGGCGGGGTTTTTTTTTT" }, { "collection": "parts", "type": "terminator", "name": "tR3", "dnasequence": "CCGTTACAAAGCGAGGCTGGGTATTTCCCGGCCTTTCTGTTATCCGAAATCCACTGAAAGCACAGCGG" }, { "collection": "parts", "type": "terminator", "name": "rrnB", "dnasequence": "CAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTAT" }, { "collection": "parts", "type": "scar", "name": "F_scar", "dnasequence": "CGCT" }, { "collection": "parts", "type": "scar", "name": "E_scar", "dnasequence": "GCTT" }, { "collection": "parts", "type": "scar", "name": "D_scar", "dnasequence": "AGGT" }, { "collection": "parts", "type": "scar", "name": "C_scar", "dnasequence": "AATG" }, { "collection": "parts", "type": "scar", "name": "B_scar", "dnasequence": "TACG" }, { "collection": "parts", "type": "scar", "name": "A_scar", "dnasequence": "GGAG" }, { "collection": "parts", "type": "scar", "name": "Y_scar", "dnasequence": "ATTG" }, { "collection": "parts", "type": "scar", "name": "X_scar", "dnasequence": "TGTC" }, { "collection": "parts", "type": "scar", "name": "V_scar", "dnasequence": "TCTG" }, { "collection": "parts", "type": "scar", "name": "U_scar", "dnasequence": "GGGC" }, { "collection": "parts", "type": "scar", "name": "R_scar", "dnasequence": "GTAA" }, { "collection": "parts", "type": "scar", "name": "Q_scar", "dnasequence": "GAGT" }, { "collection": "parts", "type": "scar", "name": "P_scar", "dnasequence": "CCTA" }, { "collection": "parts", "type": "scar", "name": "O_scar", "dnasequence": "ATGC" }, { "collection": "parts", "type": "scar", "name": "G_scar", "dnasequence": "ATAG" }, { "collection": "parts", "type": "ori", "name": "R6K", "dnasequence": "TGATTCGCACGGGCCCATGGCTAATTCCCATGTCAGCCGTTAAGTGTTCCTGTGTCACTCAAAATTGCTTTGAGAGGCTCTAAGGGCTTCTCAGTGCGTTACATCCCTGGCTTGTTGTCCACAACCGTTAAACCTTAAAAGCTTTAAAAGCCTTATATATTCTTTTTTTTCTTATAAAACTTAAAACCTTAGAGGCTATTTAAGTTGCTGATTTATATTAATTTTATTGTTCAAACATGAGAGCTTAGTACGTGAAACATGAGAGCTTAGTACGTTAGCCATGAGAGCTTAGTACGTTAGCCATGAGGGTTTAGTTCGTTAAACATGAGAGCTTAGTACGTTAAACATGAGAGCTTAGTACGTGAAACATGAGAGCTTAGTACGTACTATCAACAGGTTGAACTGCTGATCTTCAGATCCTCTACGCCGGACGCATCGTGGCCGGATCTT" }, { "collection": "parts", "type": "ori", "name": "p15a", "dnasequence": "GACCTCAGCGCTAGCGGAGTGTATACTGGCTTACTATGTTGGCACTGATGAGGGTGTCAGTGAAGTGCTTCATGTGGCAGGAGAAAAAAGGCTGCACCGGTGCGTCAGCAGAATATGTGATACAGGATATATTCCGCTTCCTCGCTCACTGACTCGCTACGCTCGGTCGTTCGACTGCGGCGAGCGGAAATGGCTTACGAACGGGGCGGAGATTTCCTGGAAGATGCCAGGAAGATACTTAACAGGGAAGTGAGAGGGCCGCGGCAAAGCCGTTTTTCCATAGGCTCCGCCCCCCTGACAAGCATCACGAAATCTGACGCTCAAATCAGTGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGCGGCTCCCTCGTGCGCTCTCCTGTTCCTGCCTTTCGGTTTACCGGTGTCATTCCGCTGTTATGGCCGCGTTTGTCTCATTCCACGCCTGACACTCAGTTCCGGGTAGGCAGTTCGCTCCAAGCTGGACTGTATGCACGAACCCCCCGTTCAGTCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGAAAGACATGCAAAAGCACCACTGGCAGCAGCCACTGGTAATTGATTTAGAGGAGTTAGTCTTGAAGTCATGCGCCGGTTAAGGCTAAACTGAAAGGACAAGTTTTGGTGACTGCGCTCCTCCAAGCCAGTTACCTCGGTTCAAAGAGTTGGTAGCTCAGAGAACCTTCGAAAAACCGCCCTGCAAGGCGGTTTTTTCGTTTTCAGAGCAAGAGATTACGCGCAGACCAAAACGATCTCAAGAAGATCATCTTATTAA" }, { "collection": "parts", "type": "ori", "name": "pSC101", "dnasequence": "AGTAAGACGGGTAAGCCTGTTGATGATACCGCTGCCTTACTGGGTGCATTAGCCAGTCTGAATGACCTGTCACGGGATAATCCGAAGTGGTCAGACTGGAAAATCAGAGGGCAGGAACTGCTGAACAGCAAAAAGTCAGATAGCACCACATAGCAGACCCGCCATAAAACGCCCTGAGAAGCCCGTGACGGGCTTTTCTTGTATTATGGGTAGTTTCCTTGCATGAATCCATAAAAGGCGCCTGTAGTGCCATTTACCCCCATTCACTGCCAGAGCCGTGAGCGCAGCGAACTGAATGTCACGAAAAAGACAGCGACTCAGGTGCCTGATGGTCGGAGACAAAAGGAATATTCAGCGATTTGCCCGAGCTTGCGAGGGTGCTACTTAAGCCTTTAGGGTTTTAAGGTCTGTTTTGTAGAGGAGCAAACAGCGTTTGCGACATCCTTTTGTAATACTGCGGAACTGACTAAAGTAGTGAGTTATACACAGGGCTGGGATCTATTCTTTTTATCTTTTTTTATTCTTTCTTTATTCTATAAATTATAACCACTTGAATATAAACAAAAAAAACACACAAAGGTCTAGCGGAATTTACAGAGGGTCTAGCAGAATTTACAAGTTTTCCAGCAAAGGTCTAGCAGAATTTACAGATACCCACAACTCAAAGGAAAAGGACTAGTAATTATCATTGACTAGCCCATCTCAATTGGTATAGTGATTAAAATCACCTAGACCAATTGAGATGTATGTCTGAATTAGTTGTTTTCAAAGCAAATGAACTAGCGATTAGTCGCTATGACTTAACGGAGCATGAAACCAAGCTAATTTTATGCTGTGTGGCACTACTCAACCCCACGATTGAAAACCCTACAAGGAAAGAACGGACGGTATCGTTCACTTATAACCAATACGCTCAGATGATGAACATCAGTAGGGAAAATGCTTATGGTGTATTAGCTAAAGCAACCAGAGAGCTGATGACGAGAACTGTGGAAATCAGGAATCCTTTGGTTAAAGGCTTTGAGATTTTCCAGTGGACAAACTATGCCAAGTTCTCAAGCGAAAAATTAGAATTAGTTTTTAGTGAAGAGATATTGCCTTATCTTTTCCAGTTAAAAAAATTCATAAAATATAATCTGGAACATGTTAAGTCTTTTGAAAACAAATACTCTATGAGGATTTATGAGTGGTTATTAAAAGAACTAACACAAAAGAAAACTCACAAGGCAAATATAGAGATTAGCCTTGATGAATTTAAGTTCATGTTAATGCTTGAAAATAACTACCATGAGTTTAAAAGGCTTAACCAATGGGTTTTGAAACCAATAAGTAAAGATTTAAACACTTACAGCAATATGAAATTGGTGGTTGATAAGCGAGGCCGCCCGACTGATACGTTGATTTTCCAAGTTGAACTAGATAGACAAATGGATCTCGTAACCGAACTTGAGAACAACCAGATAAAAATGAATGGTGACAAAATACCAACAACCATTACATCAGATTCCTACCTACATAACGGACTAAGAAAAACACTACACGATGCTTTAACTGCAAAAATTCAGCTCACCAGTTTTGAGGCAAAATTTTTGAGTGACATGCAAAGTAAGTATGATCTCAATGGTTCGTTCTCATGGCTCACGCAAAAACAACGAACCACACTAGAGAACATACTGGCTAAATACGGAAGGATCTGAGGTTCTTATGGCTCTTGTATCTATCAGTGAAGCATCAAGACTAACAAACAAAAGTAGAACAACTGTTCACCGTTACATATCAAAGGGAAAACTGTCCATATGCACAGATGAAAACGGTGTAAAAAAGATAGATACATCAGAGCTTTTACGAGTTTTTGGTGCATTCAAAGCTGTTCACCATGAACAGATCGACAATGTAACAGATGAACAGCATGTAACACCTAATAGAACAGGTGAAACCAGTAAAACAAAGCAACTAGAACATGAAATTGAACACCTGAGACAACTTGTTACAGCTCAACAGTCACACATAGACAGCCTGAAACAGGCGATGCTGCTTATCGAATCAAAGCTGCCGACAACACGGGAGCCAGTGACGCCTCCCGTGGGGAAAAAATCATGGCAATTCTGGAAGAAATAGCGCTTTCAGCCGGCAAACCGGCTGAAGCCGGATCTGCGATTCTGATAACAAACTAGCAACACCAGAACAGCCCGTTTGCGGGCAGCAAAACCCGTAC" }, { "collection": "parts", "type": "ori", "name": "pSC101ts", "dnasequence": "AGTAAGACGGGTAAGCCTGTTGATGATACCGCTGCCTTACTGGGTGCATTAGCCAGTCTGAATGACCTGTCACGGGATAATCCGAAGTGGTCAGACTGGAAAATCAGAGGGCAGGAACTGCTGAACAGCAAAAAGTCAGATAGCACCACATAGCAGACCCGCCATAAAACGCCCTGAGAAGCCCGTGACGGGCTTTTCTTGTATTATGGGTAGTTTCCTTGCATGAATCCATAAAAGGCGCCTGTAGTGCCATTTACCCCCATTCACTGCCAGAGCCGTGAGCGCAGCGAACTGAATGTCACGAAAAAGACAGCGACTCAGGTGCCTGATGGTCGGAGACAAAAGGAATATTCAGCGATTTGCCCGAGCTTGCGAGGGTGCTACTTAAGCCTTTAGGGTTTTAAGGTCTGTTTTGTAGAGGAGCAAACAGCGTTTGCGACATCCTTTTGTAATACTGCGGAACTGACTAAAGTAGTGAGTTATACACAGGGCTGGGATCTATTCTTTTTATCTTTTTTTATTCTTTCTTTATTCTATAAATTATAACCACTTGAATATAAACAAAAAAAACACACAAAGGTCTAGCGGAATTTACAGAGGGTCTAGCAGAATTTACAAGTTTTCCAGCAAAGGTCTAGCAGAATTTACAGATACCCACAACTCAAAGGAAAAGGACTAGTAATTATCATTGACTAGCCCATCTCAATTGGTATAGTGATTAAAATCACCTAGACCAATTGAGATGTATGTCTGAATTAGTTGTTTTCAAAGCAAATGAACTAGCGATTAGTCGCTATGACTTAACGGAGCATGAAACCAAGCTAATTTTATGCTGTGTGGCACTACTCAACCCCACGATTGAAAACCCTACAAGGAAAGAACGGACGGTATCGTTCACTTATAACCAATACGtTCAGATGATGAACATCAGTAGGGAAAATGCTTATGGTGTATTAGCTAAAGCAACCAGAGAGCTGATGACGAGAACTGTGGAAATCAGGAATCCTTTGGTTAAAGGCTTTGAGATTTTCCAGTGGACAAACTATGCCAAGTTCTCAAGCGAAAAATTAGAATTAGTTTTTAGTGAAGAGATATTGCCTTATCTTTTCCAGTTAAAAAAATTCATAAAATATAATCTGGAACATGTTAAGTCTTTTGAAAACAAATACTCTATGAGGATTTATGAGTGGTTATTAAAAGAACTAACACAAAAGAAAACTCACAAGGCAAATATAGAGATTAGCCTTGATGAATTTAAGTTCATGTTAATGCTTGAAAATAACTACCATGAGTTTAAAAGGCTTAACCAATGGGTTTTGAAACCAATAAGTAAAGATTTAAACACTTACAGCAATATGAAATTGGTGGTTGATAAGCGAGGCCGCCCGACTGATACGTTGATTTTCCAAGTTGAACTAGATAGACAAATGGATCTCGTAACCGAACTTGAGAACAACCAGATAAAAATGAATGGTGACAAAATACCAACAACCATTACATCAGATTCCTACCTACATAACGGACTAAGAAAAACACTACACGATGCTTTAACTGCAAAAATTCAGCTCACCAGTTTTGAGGCAAAATTTTTGAGTGACATGCAAAGTAAGTATGATCTCAATGGTTCGTTCTCATGGCTCACGCAAAAACAACGAACCACACTAGAGAACATACTGGCTAAATACGGAAGGATCTGAGGTTCTTATGGCTCTTG" }, { "collection": "parts", "type": "ori", "name": "colE1", "dnasequence": "TTTCCACAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAA" }, { "collection": "parts", "type": "recomb", "name": "attP2", "dnasequence": "GCTCATGTATGTGTCTACGCGAGATTCTCGCCCGAGAACTTCTGCAAGGCACTGCTCTTGGCT" }, { "collection": "parts", "type": "recomb", "name": "attP7", "dnasequence": "GTGTTATAAACCTGTGTGAGAGTTAAGTTTACATGCCTAACCTTAACTTTTACGCAGGTTCAGCTT" }, { "collection": "parts", "type": "recomb", "name": "attP5", "dnasequence": "CCCTAATACGCAAGTCGATAACTCTCCTGGGAGCGTTGACAACTTGCGCACCCTGATCTG" }, { "collection": "parts", "type": "oriT", "name": "oriT", "dnasequence": "AGCTTATCGGCCAGCCTCGCAGAGCAGGATTCCCGTTGAGCACCGCCAGGTGCGAATAAGGGACAGTGAAGAAGGAACACCCGCTCGCGGGTGGGCCTACTTCACCTATCCTGCCCGGCTGACGCCGTTGGATACACCAAGGAAAGTCTACACGAACCCTTTGGCAAAATCCTGTATATCGTGCGAAAAAGGATGGATATACCGAAAAAATCGCTATAATGACCCCGAAGCAGGGTTATGCAGCGGAAAGTATACCTTAA" } ]