{"date":"Thu Dec 24 00:56:10 EST 2015","genome":"NEB 10 ∆(ara-leu) 7697 araD139 fhuA ∆lacX74 galK16 galE15 e14- φ80dlacZ∆M15  recA1 relA1 endA1 nupG  rpsL (StrR) rph spoT1 ∆(mrr-hsdRMS-mcrBC)","organism":"Escherichia coli NEB 10-beta","author":["Bryan Der"],"temperature":"37","description":"TetR homologs: PhlF, SrpR, BM3R1, HlyIIR, BetI, AmtR, AmeR, QacR, IcaRA, LitR, PsrA, LmrA","growth":"Inoculation: Individual colonies into M9 media, 16 hours overnight in plate shaker.  Dilution: Next day, cells dilute ~200-fold into M9 media with antibiotics, growth for 3 hours.  Induction: Cells diluted ~650-fold into M9 media with antibiotics.  Growth: shaking incubator for 5 hours.  Arrest protein production: PBS and 2mg\/ml kanamycin.  Measurement: flow cytometry, data processing for RPU normalization.","collection":"header","media":"M9 minimal media composed of M9 media salts (6.78 g\/L Na2HPO4, 3 g\/L KH2PO4, 1 g\/L NH4Cl, 0.5 g\/L NaCl, 0.34 g\/L thiamine hydrochloride, 0.4% D-glucose, 0.2% Casamino acids, 2 mM MgSO4, and 0.1 mM CaCl2; kanamycin (50 ug\/ml), spectinomycin (50 ug\/ml)","version":"Eco1C1G1T1"}