Digitalizer
type: Collection
persistent identity: Digitalizer collection
display id: Digitalizer_collection
version: 1
description: A genetic device to digitalize gene expression into a sharp on/off signal.
OBI_0001617: 31885200
creator: Angel Goni-Moreno
creator: Chris Myers
created: 2019-10-28T11:43:58.357Z
created: 2020-06-19T14:23:27.234Z
modified: 2020-06-19T14:36:52
mutable description: Digitalizing heterologous gene expression in Gram-negative bacteria with a portable on/off module
mutable notes: Preprint at: https://www.biorxiv.org/content/10.1101/783506v1
owned by: Angel
owned by: myers
member: https://synbiohub.org/public/Digitalizer/ATGSequence/1
member: T500
member: SpeI
member: https://synbiohub.org/public/Digitalizer/PA1_04sSequence/1
member: https://synbiohub.org/public/Digitalizer/LacISequence/1
member: https://synbiohub.org/public/Digitalizer/CouplingSequence/1
member: LacI
member: https://synbiohub.org/public/Digitalizer/gfp_cassetteSequence/1
member: https://synbiohub.org/public/Digitalizer/RBS_cassetteSequence/1
member: XylSPm
member: https://synbiohub.org/public/Digitalizer/all_or_nothing/1
member: https://synbiohub.org/public/Digitalizer/Ter/1
member: https://synbiohub.org/public/Digitalizer/EcoRISequence/1
member: https://synbiohub.org/public/Digitalizer/Restriction_sitesSequence/1
member: all_or_nothing_Pm
member: T1_TE
member: NheI
member: https://synbiohub.org/public/Digitalizer/msfGFPSequence/1
member: PBS_F24
member: https://synbiohub.org/public/Digitalizer/T500Sequence/1
member: all_or_nothing_PA1_04s
member: https://synbiohub.org/public/Digitalizer/CDS/1
member: https://synbiohub.org/public/Digitalizer/MCSSequence/1
member: LacI_cassette
member: ATG
member: https://synbiohub.org/public/Digitalizer/MICc_scaffoldSequence/1
member: https://synbiohub.org/public/Digitalizer/XylSPmSequence/1
member: https://synbiohub.org/public/Digitalizer/all_or_nothing_PA1_04sSequence/1
member: Spacer
member: https://synbiohub.org/public/Digitalizer/LacI_cassetteSequence/1
member: Restriction_sites
member: https://synbiohub.org/public/Digitalizer/SpeISequence/1
member: gfp_cassette
member: https://synbiohub.org/public/Digitalizer/all_or_nothing_PmSequence/1
member: https://synbiohub.org/public/Digitalizer/sRNA_cassetteSequence/1
member: EcoRI
member: https://synbiohub.org/public/Digitalizer/SpacerSequence/1
member: PacI
member: https://synbiohub.org/public/Digitalizer/PacISequence1/1
member: Coupling
member: https://synbiohub.org/public/Digitalizer/NheISequence/1
member: sRNA_cassette
member: MICc_scaffold
member: RBS_cassette
member: https://synbiohub.org/public/Digitalizer/PBS_F24Sequence/1
member: msfGFP
member: sRNA_target
member: https://synbiohub.org/public/Digitalizer/all_or_nothingSequence/1
member: MCS
member: https://synbiohub.org/public/Digitalizer/T1_TESequence/1
member: PA1_04s_cassette
member: PA1_04s
member: https://synbiohub.org/public/Digitalizer/sRNA_targetSequence/1

Coupling
type: ComponentDefinition
persistent identity: Coupling
display id: Coupling
version: 1
description: Translational coupler
owned by: Angel
owned by: myers
type: DnaRegion
role: SO:0000804
sequence: https://synbiohub.org/public/Digitalizer/CouplingSequence/1

PBS_F24
type: ComponentDefinition
persistent identity: PBS F24
display id: PBS_F24
version: 1
owned by: Angel
owned by: myers
type: DnaRegion
role: SO:0005850
sequence: https://synbiohub.org/public/Digitalizer/PBS_F24Sequence/1

https://synbiohub.org/public/Digitalizer/Ter/1
type: ComponentDefinition
persistent identity: Ter
display id: Ter
owned by: Angel
type: DnaRegion
role: SO:0000141

RBS_cassette
type: ComponentDefinition
persistent identity: RBS cassette
display id: RBS_cassette
version: 1
description: AvrII restriction site (cctagg), overlapped with RBS (aggagga)
owned by: Angel
owned by: myers
type: DnaRegion
role: SO:0000110
sequence: https://synbiohub.org/public/Digitalizer/RBS_cassetteSequence/1

LacI
type: ComponentDefinition
persistent identity: LacI
display id: LacI
version: 1
owned by: Angel
owned by: myers
type: DnaRegion
role: SO:0000316
sequence: https://synbiohub.org/public/Digitalizer/LacISequence/1

SpeI
type: ComponentDefinition
persistent identity: SpeI
display id: SpeI
version: 1
owned by: Angel
owned by: myers
type: DnaRegion
role: SO:0001692
sequence: https://synbiohub.org/public/Digitalizer/SpeISequence/1

all_or_nothing_Pm
type: ComponentDefinition
persistent identity: all or nothing Pm
display id: all_or_nothing_Pm
version: 1
owned by: Angel
owned by: myers
type: DnaRegion
role: SO:0000804
component: https://synbiohub.org/public/Digitalizer/all_or_nothing_Pm/RBSComponent/1
component: https://synbiohub.org/public/Digitalizer/all_or_nothing_Pm/CDSComponent/1
component: https://synbiohub.org/public/Digitalizer/all_or_nothing_Pm/TerComponent/1
component: https://synbiohub.org/public/Digitalizer/all_or_nothing_Pm/MCSComponent/1
component: https://synbiohub.org/public/Digitalizer/all_or_nothing_Pm/CDS2Component/1
component: https://synbiohub.org/public/Digitalizer/all_or_nothing_Pm/Pro2Component/1
sequence annotation: https://synbiohub.org/public/Digitalizer/all_or_nothing_Pm/all_or_nothing_PmSequenceAnnotation1/1
sequence annotation: https://synbiohub.org/public/Digitalizer/all_or_nothing_Pm/all_or_nothing_PmSequenceAnnotation4/1
sequence annotation: https://synbiohub.org/public/Digitalizer/all_or_nothing_Pm/all_or_nothing_PmSequenceAnnotation3/1
sequence annotation: https://synbiohub.org/public/Digitalizer/all_or_nothing_Pm/all_or_nothing_PmSequenceAnnotation5/1
sequence annotation: https://synbiohub.org/public/Digitalizer/all_or_nothing_Pm/all_or_nothing_PmSequenceAnnotation2/1
sequence annotation: https://synbiohub.org/public/Digitalizer/all_or_nothing_Pm/all_or_nothing_PmSequenceAnnotation/1
sequence constraint: https://synbiohub.org/public/Digitalizer/all_or_nothing_Pm/SequenceConstraint1/1
sequence constraint: https://synbiohub.org/public/Digitalizer/all_or_nothing_Pm/SequenceConstraint4/1
sequence constraint: https://synbiohub.org/public/Digitalizer/all_or_nothing_Pm/SequenceConstraint/1
sequence constraint: https://synbiohub.org/public/Digitalizer/all_or_nothing_Pm/SequenceConstraint2/1
sequence constraint: https://synbiohub.org/public/Digitalizer/all_or_nothing_Pm/SequenceConstraint3/1
sequence: https://synbiohub.org/public/Digitalizer/all_or_nothing_PmSequence/1

https://synbiohub.org/public/Digitalizer/all_or_nothing_Pm/RBSComponent/1
type: Component
persistent identity: RBSComponent
display id: RBSComponent
version: 1
owned by: Angel
owned by: myers
top level: all_or_nothing_Pm
access: public
definition: RBS_cassette

https://synbiohub.org/public/Digitalizer/all_or_nothing_Pm/CDSComponent/1
type: Component
persistent identity: CDSComponent
display id: CDSComponent
version: 1
owned by: Angel
owned by: myers
top level: all_or_nothing_Pm
access: public
definition: LacI_cassette

https://synbiohub.org/public/Digitalizer/all_or_nothing_Pm/TerComponent/1
type: Component
persistent identity: TerComponent
display id: TerComponent
version: 1
owned by: Angel
owned by: myers
top level: all_or_nothing_Pm
access: public
definition: T500

https://synbiohub.org/public/Digitalizer/all_or_nothing_Pm/MCSComponent/1
type: Component
persistent identity: MCSComponent
display id: MCSComponent
version: 1
owned by: Angel
owned by: myers
top level: all_or_nothing_Pm
access: public
definition: MCS

https://synbiohub.org/public/Digitalizer/all_or_nothing_Pm/CDS2Component/1
type: Component
persistent identity: CDS2Component
display id: CDS2Component
version: 1
owned by: Angel
owned by: myers
top level: all_or_nothing_Pm
access: public
definition: gfp_cassette

https://synbiohub.org/public/Digitalizer/all_or_nothing_Pm/Pro2Component/1
type: Component
persistent identity: Pro2Component
display id: Pro2Component
version: 1
owned by: Angel
owned by: myers
top level: all_or_nothing_Pm
access: public
definition: XylSPm

https://synbiohub.org/public/Digitalizer/all_or_nothing_Pm/all_or_nothing_PmSequenceAnnotation1/1
type: SequenceAnnotation
persistent identity: all or nothing PmSequenceAnnotation1
display id: all_or_nothing_PmSequenceAnnotation1
version: 1
owned by: Angel
owned by: myers
top level: all_or_nothing_Pm
location: https://synbiohub.org/public/Digitalizer/all_or_nothing_Pm/all_or_nothing_PmSequenceAnnotation1/all_or_nothing_PmSequenceAnnotation1Range/1
component: https://synbiohub.org/public/Digitalizer/all_or_nothing_Pm/RBSComponent/1

https://synbiohub.org/public/Digitalizer/all_or_nothing_Pm/all_or_nothing_PmSequenceAnnotation1/all_or_nothing_PmSequenceAnnotation1Range/1
type: Range
version: 1
owned by: Angel
owned by: myers
top level: all_or_nothing_Pm
start: 1988
end: 1997
orientation: inline

https://synbiohub.org/public/Digitalizer/all_or_nothing_Pm/all_or_nothing_PmSequenceAnnotation4/1
type: SequenceAnnotation
persistent identity: all or nothing PmSequenceAnnotation4
display id: all_or_nothing_PmSequenceAnnotation4
version: 1
owned by: Angel
owned by: myers
top level: all_or_nothing_Pm
location: https://synbiohub.org/public/Digitalizer/all_or_nothing_Pm/all_or_nothing_PmSequenceAnnotation4/all_or_nothing_PmSequenceAnnotation4Range/1
component: https://synbiohub.org/public/Digitalizer/all_or_nothing_Pm/MCSComponent/1

https://synbiohub.org/public/Digitalizer/all_or_nothing_Pm/all_or_nothing_PmSequenceAnnotation4/all_or_nothing_PmSequenceAnnotation4Range/1
type: Range
version: 1
owned by: Angel
owned by: myers
top level: all_or_nothing_Pm
start: 3855
end: 3937
orientation: inline

https://synbiohub.org/public/Digitalizer/all_or_nothing_Pm/all_or_nothing_PmSequenceAnnotation3/1
type: SequenceAnnotation
persistent identity: all or nothing PmSequenceAnnotation3
display id: all_or_nothing_PmSequenceAnnotation3
version: 1
owned by: Angel
owned by: myers
top level: all_or_nothing_Pm
location: https://synbiohub.org/public/Digitalizer/all_or_nothing_Pm/all_or_nothing_PmSequenceAnnotation3/all_or_nothing_PmSequenceAnnotation3Range/1
component: https://synbiohub.org/public/Digitalizer/all_or_nothing_Pm/CDS2Component/1

https://synbiohub.org/public/Digitalizer/all_or_nothing_Pm/all_or_nothing_PmSequenceAnnotation3/all_or_nothing_PmSequenceAnnotation3Range/1
type: Range
version: 1
owned by: Angel
owned by: myers
top level: all_or_nothing_Pm
start: 3126
end: 3854
orientation: inline

https://synbiohub.org/public/Digitalizer/all_or_nothing_Pm/all_or_nothing_PmSequenceAnnotation5/1
type: SequenceAnnotation
persistent identity: all or nothing PmSequenceAnnotation5
display id: all_or_nothing_PmSequenceAnnotation5
version: 1
owned by: Angel
owned by: myers
top level: all_or_nothing_Pm
location: https://synbiohub.org/public/Digitalizer/all_or_nothing_Pm/all_or_nothing_PmSequenceAnnotation5/all_or_nothing_PmSequenceAnnotation5Range/1
component: https://synbiohub.org/public/Digitalizer/all_or_nothing_Pm/TerComponent/1

https://synbiohub.org/public/Digitalizer/all_or_nothing_Pm/all_or_nothing_PmSequenceAnnotation5/all_or_nothing_PmSequenceAnnotation5Range/1
type: Range
version: 1
owned by: Angel
owned by: myers
top level: all_or_nothing_Pm
start: 3938
end: 3967
orientation: inline

https://synbiohub.org/public/Digitalizer/all_or_nothing_Pm/all_or_nothing_PmSequenceAnnotation2/1
type: SequenceAnnotation
persistent identity: all or nothing PmSequenceAnnotation2
display id: all_or_nothing_PmSequenceAnnotation2
version: 1
owned by: Angel
owned by: myers
top level: all_or_nothing_Pm
location: https://synbiohub.org/public/Digitalizer/all_or_nothing_Pm/all_or_nothing_PmSequenceAnnotation2/all_or_nothing_PmSequenceAnnotation2Range/1
component: https://synbiohub.org/public/Digitalizer/all_or_nothing_Pm/CDSComponent/1

https://synbiohub.org/public/Digitalizer/all_or_nothing_Pm/all_or_nothing_PmSequenceAnnotation2/all_or_nothing_PmSequenceAnnotation2Range/1
type: Range
version: 1
owned by: Angel
owned by: myers
top level: all_or_nothing_Pm
start: 1998
end: 3125
orientation: inline

https://synbiohub.org/public/Digitalizer/all_or_nothing_Pm/all_or_nothing_PmSequenceAnnotation/1
type: SequenceAnnotation
persistent identity: all or nothing PmSequenceAnnotation
display id: all_or_nothing_PmSequenceAnnotation
version: 1
owned by: Angel
owned by: myers
top level: all_or_nothing_Pm
location: https://synbiohub.org/public/Digitalizer/all_or_nothing_Pm/all_or_nothing_PmSequenceAnnotation/all_or_nothing_PmSequenceAnnotationRange/1
component: https://synbiohub.org/public/Digitalizer/all_or_nothing_Pm/Pro2Component/1

https://synbiohub.org/public/Digitalizer/all_or_nothing_Pm/all_or_nothing_PmSequenceAnnotation/all_or_nothing_PmSequenceAnnotationRange/1
type: Range
persistent identity: all or nothing PmSequenceAnnotationRange
display id: all_or_nothing_PmSequenceAnnotationRange
version: 1
owned by: Angel
owned by: myers
top level: all_or_nothing_Pm
start: 1
end: 1987
orientation: inline

https://synbiohub.org/public/Digitalizer/all_or_nothing_Pm/SequenceConstraint1/1
type: SequenceConstraint
persistent identity: SequenceConstraint1
display id: SequenceConstraint1
version: 1
owned by: Angel
owned by: myers
top level: all_or_nothing_Pm
restriction: precedes
subject: https://synbiohub.org/public/Digitalizer/all_or_nothing_Pm/RBSComponent/1
object: https://synbiohub.org/public/Digitalizer/all_or_nothing_Pm/CDSComponent/1

https://synbiohub.org/public/Digitalizer/all_or_nothing_Pm/SequenceConstraint4/1
type: SequenceConstraint
persistent identity: SequenceConstraint4
display id: SequenceConstraint4
version: 1
owned by: Angel
owned by: myers
top level: all_or_nothing_Pm
restriction: precedes
subject: https://synbiohub.org/public/Digitalizer/all_or_nothing_Pm/MCSComponent/1
object: https://synbiohub.org/public/Digitalizer/all_or_nothing_Pm/TerComponent/1

https://synbiohub.org/public/Digitalizer/all_or_nothing_Pm/SequenceConstraint/1
type: SequenceConstraint
persistent identity: SequenceConstraint
display id: SequenceConstraint
version: 1
owned by: Angel
owned by: myers
top level: all_or_nothing_Pm
restriction: precedes
subject: https://synbiohub.org/public/Digitalizer/all_or_nothing_Pm/Pro2Component/1
object: https://synbiohub.org/public/Digitalizer/all_or_nothing_Pm/RBSComponent/1

https://synbiohub.org/public/Digitalizer/all_or_nothing_Pm/SequenceConstraint2/1
type: SequenceConstraint
persistent identity: SequenceConstraint2
display id: SequenceConstraint2
version: 1
owned by: Angel
owned by: myers
top level: all_or_nothing_Pm
restriction: precedes
subject: https://synbiohub.org/public/Digitalizer/all_or_nothing_Pm/CDSComponent/1
object: https://synbiohub.org/public/Digitalizer/all_or_nothing_Pm/CDS2Component/1

https://synbiohub.org/public/Digitalizer/all_or_nothing_Pm/SequenceConstraint3/1
type: SequenceConstraint
persistent identity: SequenceConstraint3
display id: SequenceConstraint3
version: 1
owned by: Angel
owned by: myers
top level: all_or_nothing_Pm
restriction: precedes
subject: https://synbiohub.org/public/Digitalizer/all_or_nothing_Pm/CDS2Component/1
object: https://synbiohub.org/public/Digitalizer/all_or_nothing_Pm/MCSComponent/1

Restriction_sites
type: ComponentDefinition
persistent identity: Restriction sites
display id: Restriction_sites
version: 1
description: SacI - KpnI - SmaI - BamHI - XbaI - SalI - PstI - SphI - HindIII - NotI
owned by: Angel
owned by: myers
type: DnaRegion
role: SO:0000804
sequence: https://synbiohub.org/public/Digitalizer/Restriction_sitesSequence/1

PacI
type: ComponentDefinition
persistent identity: PacI
display id: PacI
version: 1
owned by: Angel
owned by: myers
type: DnaRegion
role: SO:0001692
sequence: https://synbiohub.org/public/Digitalizer/PacISequence1/1

gfp_cassette
type: ComponentDefinition
persistent identity: gfp cassette
display id: gfp_cassette
version: 1
owned by: Angel
owned by: myers
type: DnaRegion
role: SO:0000316
component: https://synbiohub.org/public/Digitalizer/gfp_cassette/ATGComponent/1
component: https://synbiohub.org/public/Digitalizer/gfp_cassette/CUT3Component/1
component: https://synbiohub.org/public/Digitalizer/gfp_cassette/CUT2Component/1
component: https://synbiohub.org/public/Digitalizer/gfp_cassette/CDS3Component/1
sequence annotation: https://synbiohub.org/public/Digitalizer/gfp_cassette/gfp_cassetteSequenceAnnotation3/1
sequence annotation: https://synbiohub.org/public/Digitalizer/gfp_cassette/gfp_cassetteSequenceAnnotation1/1
sequence annotation: https://synbiohub.org/public/Digitalizer/gfp_cassette/gfp_cassetteSequenceAnnotation2/1
sequence annotation: https://synbiohub.org/public/Digitalizer/gfp_cassette/gfp_cassetteSequenceAnnotation/1
sequence constraint: https://synbiohub.org/public/Digitalizer/gfp_cassette/SequenceConstraint2/1
sequence constraint: https://synbiohub.org/public/Digitalizer/gfp_cassette/SequenceConstraint1/1
sequence constraint: https://synbiohub.org/public/Digitalizer/gfp_cassette/SequenceConstraint/1
sequence: https://synbiohub.org/public/Digitalizer/gfp_cassetteSequence/1

https://synbiohub.org/public/Digitalizer/gfp_cassette/ATGComponent/1
type: Component
persistent identity: ATGComponent
display id: ATGComponent
version: 1
owned by: Angel
owned by: myers
top level: gfp_cassette
access: public
definition: ATG

https://synbiohub.org/public/Digitalizer/gfp_cassette/CUT3Component/1
type: Component
persistent identity: CUT3Component
display id: CUT3Component
version: 1
owned by: Angel
owned by: myers
top level: gfp_cassette
access: public
definition: EcoRI

https://synbiohub.org/public/Digitalizer/gfp_cassette/CUT2Component/1
type: Component
persistent identity: CUT2Component
display id: CUT2Component
version: 1
owned by: Angel
owned by: myers
top level: gfp_cassette
access: public
definition: NheI

https://synbiohub.org/public/Digitalizer/gfp_cassette/CDS3Component/1
type: Component
persistent identity: CDS3Component
display id: CDS3Component
version: 1
owned by: Angel
owned by: myers
top level: gfp_cassette
access: public
definition: msfGFP

https://synbiohub.org/public/Digitalizer/gfp_cassette/gfp_cassetteSequenceAnnotation3/1
type: SequenceAnnotation
persistent identity: gfp cassetteSequenceAnnotation3
display id: gfp_cassetteSequenceAnnotation3
version: 1
owned by: Angel
owned by: myers
top level: gfp_cassette
location: https://synbiohub.org/public/Digitalizer/gfp_cassette/gfp_cassetteSequenceAnnotation3/gfp_cassetteSequenceAnnotation3Range/1
component: https://synbiohub.org/public/Digitalizer/gfp_cassette/CUT3Component/1

https://synbiohub.org/public/Digitalizer/gfp_cassette/gfp_cassetteSequenceAnnotation3/gfp_cassetteSequenceAnnotation3Range/1
type: Range
persistent identity: gfp cassetteSequenceAnnotation3Range
display id: gfp_cassetteSequenceAnnotation3Range
version: 1
owned by: Angel
owned by: myers
top level: gfp_cassette
start: 724
end: 729
orientation: inline

https://synbiohub.org/public/Digitalizer/gfp_cassette/gfp_cassetteSequenceAnnotation1/1
type: SequenceAnnotation
persistent identity: gfp cassetteSequenceAnnotation1
display id: gfp_cassetteSequenceAnnotation1
version: 1
owned by: Angel
owned by: myers
top level: gfp_cassette
location: https://synbiohub.org/public/Digitalizer/gfp_cassette/gfp_cassetteSequenceAnnotation1/gfp_cassetteSequenceAnnotation1Range/1
component: https://synbiohub.org/public/Digitalizer/gfp_cassette/CUT2Component/1

https://synbiohub.org/public/Digitalizer/gfp_cassette/gfp_cassetteSequenceAnnotation1/gfp_cassetteSequenceAnnotation1Range/1
type: Range
persistent identity: gfp cassetteSequenceAnnotation1Range
display id: gfp_cassetteSequenceAnnotation1Range
version: 1
owned by: Angel
owned by: myers
top level: gfp_cassette
start: 4
end: 9
orientation: inline

https://synbiohub.org/public/Digitalizer/gfp_cassette/gfp_cassetteSequenceAnnotation2/1
type: SequenceAnnotation
persistent identity: gfp cassetteSequenceAnnotation2
display id: gfp_cassetteSequenceAnnotation2
version: 1
owned by: Angel
owned by: myers
top level: gfp_cassette
location: https://synbiohub.org/public/Digitalizer/gfp_cassette/gfp_cassetteSequenceAnnotation2/gfp_cassetteSequenceAnnotation2Range/1
component: https://synbiohub.org/public/Digitalizer/gfp_cassette/CDS3Component/1

https://synbiohub.org/public/Digitalizer/gfp_cassette/gfp_cassetteSequenceAnnotation2/gfp_cassetteSequenceAnnotation2Range/1
type: Range
persistent identity: gfp cassetteSequenceAnnotation2Range
display id: gfp_cassetteSequenceAnnotation2Range
version: 1
owned by: Angel
owned by: myers
top level: gfp_cassette
start: 10
end: 723
orientation: inline

https://synbiohub.org/public/Digitalizer/gfp_cassette/gfp_cassetteSequenceAnnotation/1
type: SequenceAnnotation
persistent identity: gfp cassetteSequenceAnnotation
display id: gfp_cassetteSequenceAnnotation
version: 1
owned by: Angel
owned by: myers
top level: gfp_cassette
location: https://synbiohub.org/public/Digitalizer/gfp_cassette/gfp_cassetteSequenceAnnotation/gfp_cassetteSequenceAnnotationRange/1
component: https://synbiohub.org/public/Digitalizer/gfp_cassette/ATGComponent/1

https://synbiohub.org/public/Digitalizer/gfp_cassette/gfp_cassetteSequenceAnnotation/gfp_cassetteSequenceAnnotationRange/1
type: Range
persistent identity: gfp cassetteSequenceAnnotationRange
display id: gfp_cassetteSequenceAnnotationRange
version: 1
owned by: Angel
owned by: myers
top level: gfp_cassette
start: 1
end: 3
orientation: inline

https://synbiohub.org/public/Digitalizer/gfp_cassette/SequenceConstraint2/1
type: SequenceConstraint
persistent identity: SequenceConstraint2
display id: SequenceConstraint2
version: 1
owned by: Angel
owned by: myers
top level: gfp_cassette
restriction: precedes
subject: https://synbiohub.org/public/Digitalizer/gfp_cassette/CDS3Component/1
object: https://synbiohub.org/public/Digitalizer/gfp_cassette/CUT3Component/1

https://synbiohub.org/public/Digitalizer/gfp_cassette/SequenceConstraint1/1
type: SequenceConstraint
persistent identity: SequenceConstraint1
display id: SequenceConstraint1
version: 1
owned by: Angel
owned by: myers
top level: gfp_cassette
restriction: precedes
subject: https://synbiohub.org/public/Digitalizer/gfp_cassette/CUT2Component/1
object: https://synbiohub.org/public/Digitalizer/gfp_cassette/CDS3Component/1

https://synbiohub.org/public/Digitalizer/gfp_cassette/SequenceConstraint/1
type: SequenceConstraint
persistent identity: SequenceConstraint
display id: SequenceConstraint
version: 1
owned by: Angel
owned by: myers
top level: gfp_cassette
restriction: precedes
subject: https://synbiohub.org/public/Digitalizer/gfp_cassette/ATGComponent/1
object: https://synbiohub.org/public/Digitalizer/gfp_cassette/CUT2Component/1

sRNA_target
type: ComponentDefinition
persistent identity: sRNA target
display id: sRNA_target
version: 1
owned by: Angel
owned by: myers
type: DnaRegion
role: SO:0000804
sequence: https://synbiohub.org/public/Digitalizer/sRNA_targetSequence/1

sRNA_cassette
type: ComponentDefinition
persistent identity: sRNA cassette
display id: sRNA_cassette
version: 1
owned by: Angel
owned by: myers
type: DnaRegion
role: SO:0000316
component: https://synbiohub.org/public/Digitalizer/sRNA_cassette/MICc_scaffoldComponent/1
component: https://synbiohub.org/public/Digitalizer/sRNA_cassette/sRNA_targetComponent/1
sequence annotation: https://synbiohub.org/public/Digitalizer/sRNA_cassette/sRNA_cassetteSequenceAnnotation1/1
sequence annotation: https://synbiohub.org/public/Digitalizer/sRNA_cassette/sRNA_cassetteSequenceAnnotation/1
sequence constraint: https://synbiohub.org/public/Digitalizer/sRNA_cassette/SequenceConstraint/1
sequence: https://synbiohub.org/public/Digitalizer/sRNA_cassetteSequence/1

https://synbiohub.org/public/Digitalizer/sRNA_cassette/MICc_scaffoldComponent/1
type: Component
persistent identity: MICc scaffoldComponent
display id: MICc_scaffoldComponent
version: 1
owned by: Angel
owned by: myers
top level: sRNA_cassette
access: public
definition: MICc_scaffold

https://synbiohub.org/public/Digitalizer/sRNA_cassette/sRNA_targetComponent/1
type: Component
persistent identity: sRNA targetComponent
display id: sRNA_targetComponent
version: 1
owned by: Angel
owned by: myers
top level: sRNA_cassette
access: public
definition: sRNA_target

https://synbiohub.org/public/Digitalizer/sRNA_cassette/sRNA_cassetteSequenceAnnotation1/1
type: SequenceAnnotation
persistent identity: sRNA cassetteSequenceAnnotation1
display id: sRNA_cassetteSequenceAnnotation1
version: 1
owned by: Angel
owned by: myers
top level: sRNA_cassette
location: https://synbiohub.org/public/Digitalizer/sRNA_cassette/sRNA_cassetteSequenceAnnotation1/sRNA_cassetteSequenceAnnotation1Range/1
component: https://synbiohub.org/public/Digitalizer/sRNA_cassette/MICc_scaffoldComponent/1

https://synbiohub.org/public/Digitalizer/sRNA_cassette/sRNA_cassetteSequenceAnnotation1/sRNA_cassetteSequenceAnnotation1Range/1
type: Range
persistent identity: sRNA cassetteSequenceAnnotation1Range
display id: sRNA_cassetteSequenceAnnotation1Range
version: 1
owned by: Angel
owned by: myers
top level: sRNA_cassette
start: 24
end: 108
orientation: inline

https://synbiohub.org/public/Digitalizer/sRNA_cassette/sRNA_cassetteSequenceAnnotation/1
type: SequenceAnnotation
persistent identity: sRNA cassetteSequenceAnnotation
display id: sRNA_cassetteSequenceAnnotation
version: 1
owned by: Angel
owned by: myers
top level: sRNA_cassette
location: https://synbiohub.org/public/Digitalizer/sRNA_cassette/sRNA_cassetteSequenceAnnotation/sRNA_cassetteSequenceAnnotationRange/1
component: https://synbiohub.org/public/Digitalizer/sRNA_cassette/sRNA_targetComponent/1

https://synbiohub.org/public/Digitalizer/sRNA_cassette/sRNA_cassetteSequenceAnnotation/sRNA_cassetteSequenceAnnotationRange/1
type: Range
persistent identity: sRNA cassetteSequenceAnnotationRange
display id: sRNA_cassetteSequenceAnnotationRange
version: 1
owned by: Angel
owned by: myers
top level: sRNA_cassette
start: 1
end: 23
orientation: inline

https://synbiohub.org/public/Digitalizer/sRNA_cassette/SequenceConstraint/1
type: SequenceConstraint
persistent identity: SequenceConstraint
display id: SequenceConstraint
version: 1
owned by: Angel
owned by: myers
top level: sRNA_cassette
restriction: precedes
subject: https://synbiohub.org/public/Digitalizer/sRNA_cassette/sRNA_targetComponent/1
object: https://synbiohub.org/public/Digitalizer/sRNA_cassette/MICc_scaffoldComponent/1

https://synbiohub.org/public/Digitalizer/CDS/1
type: ComponentDefinition
persistent identity: CDS
display id: CDS
owned by: Angel
type: DnaRegion
role: SO:0000316

msfGFP
type: ComponentDefinition
persistent identity: msfGFP
display id: msfGFP
version: 1
owned by: Angel
owned by: myers
type: DnaRegion
role: SO:0000316
sequence: https://synbiohub.org/public/Digitalizer/msfGFPSequence/1

MICc_scaffold
type: ComponentDefinition
persistent identity: MICc scaffold
display id: MICc_scaffold
version: 1
owned by: Angel
owned by: myers
type: DnaRegion
role: SO:0000804
sequence: https://synbiohub.org/public/Digitalizer/MICc_scaffoldSequence/1

PA1_04s
type: ComponentDefinition
persistent identity: PA1 04s
display id: PA1_04s
version: 1
owned by: Angel
owned by: myers
type: DnaRegion
role: SO:0000167
sequence: https://synbiohub.org/public/Digitalizer/PA1_04sSequence/1

https://synbiohub.org/public/Digitalizer/all_or_nothing/1
type: ComponentDefinition
persistent identity: all or nothing
display id: all_or_nothing
version: 1
owned by: Angel
owned by: myers
type: DnaRegion
role: SO:0000804
component: https://synbiohub.org/public/Digitalizer/all_or_nothing/PA1_04s_cassetteComponent/1
component: https://synbiohub.org/public/Digitalizer/all_or_nothing/CUT1Component/1
component: https://synbiohub.org/public/Digitalizer/all_or_nothing/Pm_cassetteComponent/1
component: https://synbiohub.org/public/Digitalizer/all_or_nothing/CUTComponent/1
sequence annotation: https://synbiohub.org/public/Digitalizer/all_or_nothing/all_or_nothingSequenceAnnotation2/1
sequence annotation: https://synbiohub.org/public/Digitalizer/all_or_nothing/all_or_nothingSequenceAnnotation3/1
sequence annotation: https://synbiohub.org/public/Digitalizer/all_or_nothing/all_or_nothingSequenceAnnotation1/1
sequence annotation: https://synbiohub.org/public/Digitalizer/all_or_nothing/all_or_nothingSequenceAnnotation/1
sequence constraint: https://synbiohub.org/public/Digitalizer/all_or_nothing/SequenceConstraint1/1
sequence constraint: https://synbiohub.org/public/Digitalizer/all_or_nothing/SequenceConstraint/1
sequence constraint: https://synbiohub.org/public/Digitalizer/all_or_nothing/SequenceConstraint2/1
sequence: https://synbiohub.org/public/Digitalizer/all_or_nothingSequence/1

https://synbiohub.org/public/Digitalizer/all_or_nothing/PA1_04s_cassetteComponent/1
type: Component
persistent identity: PA1 04s cassetteComponent
display id: PA1_04s_cassetteComponent
version: 1
owned by: Angel
owned by: myers
top level: https://synbiohub.org/public/Digitalizer/all_or_nothing/1
access: public
definition: all_or_nothing_PA1_04s

https://synbiohub.org/public/Digitalizer/all_or_nothing/CUT1Component/1
type: Component
persistent identity: CUT1Component
display id: CUT1Component
version: 1
owned by: Angel
owned by: myers
top level: https://synbiohub.org/public/Digitalizer/all_or_nothing/1
access: public
definition: PacI

https://synbiohub.org/public/Digitalizer/all_or_nothing/Pm_cassetteComponent/1
type: Component
persistent identity: Pm cassetteComponent
display id: Pm_cassetteComponent
version: 1
owned by: Angel
owned by: myers
top level: https://synbiohub.org/public/Digitalizer/all_or_nothing/1
access: public
definition: all_or_nothing_Pm

https://synbiohub.org/public/Digitalizer/all_or_nothing/CUTComponent/1
type: Component
persistent identity: CUTComponent
display id: CUTComponent
version: 1
owned by: Angel
owned by: myers
top level: https://synbiohub.org/public/Digitalizer/all_or_nothing/1
access: public
definition: SpeI

https://synbiohub.org/public/Digitalizer/all_or_nothing/all_or_nothingSequenceAnnotation2/1
type: SequenceAnnotation
persistent identity: all or nothingSequenceAnnotation2
display id: all_or_nothingSequenceAnnotation2
version: 1
owned by: Angel
owned by: myers
top level: https://synbiohub.org/public/Digitalizer/all_or_nothing/1
location: https://synbiohub.org/public/Digitalizer/all_or_nothing/all_or_nothingSequenceAnnotation2/all_or_nothingSequenceAnnotation2Range/1
component: https://synbiohub.org/public/Digitalizer/all_or_nothing/CUTComponent/1

https://synbiohub.org/public/Digitalizer/all_or_nothing/all_or_nothingSequenceAnnotation2/all_or_nothingSequenceAnnotation2Range/1
type: Range
persistent identity: all or nothingSequenceAnnotation2Range
display id: all_or_nothingSequenceAnnotation2Range
version: 1
owned by: Angel
owned by: myers
top level: https://synbiohub.org/public/Digitalizer/all_or_nothing/1
start: 4271
end: 4276
orientation: inline

https://synbiohub.org/public/Digitalizer/all_or_nothing/all_or_nothingSequenceAnnotation3/1
type: SequenceAnnotation
persistent identity: all or nothingSequenceAnnotation3
display id: all_or_nothingSequenceAnnotation3
version: 1
owned by: Angel
owned by: myers
top level: https://synbiohub.org/public/Digitalizer/all_or_nothing/1
location: https://synbiohub.org/public/Digitalizer/all_or_nothing/all_or_nothingSequenceAnnotation3/all_or_nothingSequenceAnnotation3Range/1
component: https://synbiohub.org/public/Digitalizer/all_or_nothing/CUT1Component/1

https://synbiohub.org/public/Digitalizer/all_or_nothing/all_or_nothingSequenceAnnotation3/all_or_nothingSequenceAnnotation3Range/1
type: Range
persistent identity: all or nothingSequenceAnnotation3Range
display id: all_or_nothingSequenceAnnotation3Range
version: 1
owned by: Angel
owned by: myers
top level: https://synbiohub.org/public/Digitalizer/all_or_nothing/1
start: 1
end: 8
orientation: inline

https://synbiohub.org/public/Digitalizer/all_or_nothing/all_or_nothingSequenceAnnotation1/1
type: SequenceAnnotation
persistent identity: all or nothingSequenceAnnotation1
display id: all_or_nothingSequenceAnnotation1
version: 1
owned by: Angel
owned by: myers
top level: https://synbiohub.org/public/Digitalizer/all_or_nothing/1
location: https://synbiohub.org/public/Digitalizer/all_or_nothing/all_or_nothingSequenceAnnotation1/all_or_nothingSequenceAnnotation1Range/1
component: https://synbiohub.org/public/Digitalizer/all_or_nothing/PA1_04s_cassetteComponent/1

https://synbiohub.org/public/Digitalizer/all_or_nothing/all_or_nothingSequenceAnnotation1/all_or_nothingSequenceAnnotation1Range/1
type: Range
persistent identity: all or nothingSequenceAnnotation1Range
display id: all_or_nothingSequenceAnnotation1Range
version: 1
owned by: Angel
owned by: myers
top level: https://synbiohub.org/public/Digitalizer/all_or_nothing/1
start: 3976
end: 4270
orientation: reverseComplement

https://synbiohub.org/public/Digitalizer/all_or_nothing/all_or_nothingSequenceAnnotation/1
type: SequenceAnnotation
persistent identity: all or nothingSequenceAnnotation
display id: all_or_nothingSequenceAnnotation
version: 1
owned by: Angel
owned by: myers
top level: https://synbiohub.org/public/Digitalizer/all_or_nothing/1
location: https://synbiohub.org/public/Digitalizer/all_or_nothing/all_or_nothingSequenceAnnotation/all_or_nothingSequenceAnnotationRange/1
component: https://synbiohub.org/public/Digitalizer/all_or_nothing/Pm_cassetteComponent/1

https://synbiohub.org/public/Digitalizer/all_or_nothing/all_or_nothingSequenceAnnotation/all_or_nothingSequenceAnnotationRange/1
type: Range
persistent identity: all or nothingSequenceAnnotationRange
display id: all_or_nothingSequenceAnnotationRange
version: 1
owned by: Angel
owned by: myers
top level: https://synbiohub.org/public/Digitalizer/all_or_nothing/1
start: 9
end: 3975
orientation: inline

https://synbiohub.org/public/Digitalizer/all_or_nothing/SequenceConstraint1/1
type: SequenceConstraint
persistent identity: SequenceConstraint1
display id: SequenceConstraint1
version: 1
owned by: Angel
owned by: myers
top level: https://synbiohub.org/public/Digitalizer/all_or_nothing/1
restriction: precedes
subject: https://synbiohub.org/public/Digitalizer/all_or_nothing/Pm_cassetteComponent/1
object: https://synbiohub.org/public/Digitalizer/all_or_nothing/PA1_04s_cassetteComponent/1

https://synbiohub.org/public/Digitalizer/all_or_nothing/SequenceConstraint/1
type: SequenceConstraint
persistent identity: SequenceConstraint
display id: SequenceConstraint
version: 1
owned by: Angel
owned by: myers
top level: https://synbiohub.org/public/Digitalizer/all_or_nothing/1
restriction: precedes
subject: https://synbiohub.org/public/Digitalizer/all_or_nothing/CUT1Component/1
object: https://synbiohub.org/public/Digitalizer/all_or_nothing/Pm_cassetteComponent/1

https://synbiohub.org/public/Digitalizer/all_or_nothing/SequenceConstraint2/1
type: SequenceConstraint
persistent identity: SequenceConstraint2
display id: SequenceConstraint2
version: 1
owned by: Angel
owned by: myers
top level: https://synbiohub.org/public/Digitalizer/all_or_nothing/1
restriction: precedes
subject: https://synbiohub.org/public/Digitalizer/all_or_nothing/PA1_04s_cassetteComponent/1
object: https://synbiohub.org/public/Digitalizer/all_or_nothing/CUTComponent/1

Spacer
type: ComponentDefinition
persistent identity: Spacer
display id: Spacer
version: 1
owned by: Angel
owned by: myers
type: DnaRegion
role: SO:0000804
sequence: https://synbiohub.org/public/Digitalizer/SpacerSequence/1

all_or_nothing_PA1_04s
type: ComponentDefinition
persistent identity: all or nothing PA1 04s
display id: all_or_nothing_PA1_04s
version: 1
owned by: Angel
owned by: myers
type: DnaRegion
role: SO:0000804
component: https://synbiohub.org/public/Digitalizer/all_or_nothing_PA1_04s/CDSComponent1/1
component: https://synbiohub.org/public/Digitalizer/all_or_nothing_PA1_04s/TerComponent/1
component: https://synbiohub.org/public/Digitalizer/all_or_nothing_PA1_04s/ProComponent/1
sequence annotation: https://synbiohub.org/public/Digitalizer/all_or_nothing_PA1_04s/all_or_nothing_PA1_04sSequenceAnnotation1/1
sequence annotation: https://synbiohub.org/public/Digitalizer/all_or_nothing_PA1_04s/all_or_nothing_PA1_04sSequenceAnnotation2/1
sequence annotation: https://synbiohub.org/public/Digitalizer/all_or_nothing_PA1_04s/all_or_nothing_PA1_04sSequenceAnnotation/1
sequence constraint: https://synbiohub.org/public/Digitalizer/all_or_nothing_PA1_04s/SequenceConstraint1/1
sequence constraint: https://synbiohub.org/public/Digitalizer/all_or_nothing_PA1_04s/SequenceConstraint/1
sequence: https://synbiohub.org/public/Digitalizer/all_or_nothing_PA1_04sSequence/1

https://synbiohub.org/public/Digitalizer/all_or_nothing_PA1_04s/CDSComponent1/1
type: Component
persistent identity: CDSComponent1
display id: CDSComponent1
version: 1
owned by: Angel
owned by: myers
top level: all_or_nothing_PA1_04s
access: public
definition: sRNA_cassette

https://synbiohub.org/public/Digitalizer/all_or_nothing_PA1_04s/TerComponent/1
type: Component
persistent identity: TerComponent
display id: TerComponent
version: 1
owned by: Angel
owned by: myers
top level: all_or_nothing_PA1_04s
access: public
definition: T1_TE

https://synbiohub.org/public/Digitalizer/all_or_nothing_PA1_04s/ProComponent/1
type: Component
persistent identity: ProComponent
display id: ProComponent
version: 1
owned by: Angel
owned by: myers
top level: all_or_nothing_PA1_04s
access: public
definition: PA1_04s

https://synbiohub.org/public/Digitalizer/all_or_nothing_PA1_04s/all_or_nothing_PA1_04sSequenceAnnotation1/1
type: SequenceAnnotation
version: 1
owned by: Angel
owned by: myers
top level: all_or_nothing_PA1_04s
location: https://synbiohub.org/public/Digitalizer/all_or_nothing_PA1_04s/all_or_nothing_PA1_04sSequenceAnnotation1/all_or_nothing_PA1_04sSequenceAnnotation1Range/1
component: https://synbiohub.org/public/Digitalizer/all_or_nothing_PA1_04s/CDSComponent1/1

https://synbiohub.org/public/Digitalizer/all_or_nothing_PA1_04s/all_or_nothing_PA1_04sSequenceAnnotation1/all_or_nothing_PA1_04sSequenceAnnotation1Range/1
type: Range
version: 1
owned by: Angel
owned by: myers
top level: all_or_nothing_PA1_04s
start: 54
end: 161
orientation: inline

https://synbiohub.org/public/Digitalizer/all_or_nothing_PA1_04s/all_or_nothing_PA1_04sSequenceAnnotation2/1
type: SequenceAnnotation
version: 1
owned by: Angel
owned by: myers
top level: all_or_nothing_PA1_04s
location: https://synbiohub.org/public/Digitalizer/all_or_nothing_PA1_04s/all_or_nothing_PA1_04sSequenceAnnotation2/all_or_nothing_PA1_04sSequenceAnnotation2Range/1
component: https://synbiohub.org/public/Digitalizer/all_or_nothing_PA1_04s/TerComponent/1

https://synbiohub.org/public/Digitalizer/all_or_nothing_PA1_04s/all_or_nothing_PA1_04sSequenceAnnotation2/all_or_nothing_PA1_04sSequenceAnnotation2Range/1
type: Range
version: 1
owned by: Angel
owned by: myers
top level: all_or_nothing_PA1_04s
start: 162
end: 295
orientation: inline

https://synbiohub.org/public/Digitalizer/all_or_nothing_PA1_04s/all_or_nothing_PA1_04sSequenceAnnotation/1
type: SequenceAnnotation
version: 1
owned by: Angel
owned by: myers
top level: all_or_nothing_PA1_04s
location: https://synbiohub.org/public/Digitalizer/all_or_nothing_PA1_04s/all_or_nothing_PA1_04sSequenceAnnotation/all_or_nothing_PA1_04sSequenceAnnotationRange/1
component: https://synbiohub.org/public/Digitalizer/all_or_nothing_PA1_04s/ProComponent/1

https://synbiohub.org/public/Digitalizer/all_or_nothing_PA1_04s/all_or_nothing_PA1_04sSequenceAnnotation/all_or_nothing_PA1_04sSequenceAnnotationRange/1
type: Range
version: 1
owned by: Angel
owned by: myers
top level: all_or_nothing_PA1_04s
start: 1
end: 53
orientation: inline

https://synbiohub.org/public/Digitalizer/all_or_nothing_PA1_04s/SequenceConstraint1/1
type: SequenceConstraint
persistent identity: SequenceConstraint1
display id: SequenceConstraint1
version: 1
owned by: Angel
owned by: myers
top level: all_or_nothing_PA1_04s
restriction: precedes
subject: https://synbiohub.org/public/Digitalizer/all_or_nothing_PA1_04s/CDSComponent1/1
object: https://synbiohub.org/public/Digitalizer/all_or_nothing_PA1_04s/TerComponent/1

https://synbiohub.org/public/Digitalizer/all_or_nothing_PA1_04s/SequenceConstraint/1
type: SequenceConstraint
persistent identity: SequenceConstraint
display id: SequenceConstraint
version: 1
owned by: Angel
owned by: myers
top level: all_or_nothing_PA1_04s
restriction: precedes
subject: https://synbiohub.org/public/Digitalizer/all_or_nothing_PA1_04s/ProComponent/1
object: https://synbiohub.org/public/Digitalizer/all_or_nothing_PA1_04s/CDSComponent1/1

PA1_04s_cassette
type: ComponentDefinition
persistent identity: PA1 04s cassette
display id: PA1_04s_cassette
owned by: Angel
type: DnaRegion
role: SO:0000167
component: https://synbiohub.org/public/Digitalizer/PA1_04s_cassette/CDSComponent/1
component: https://synbiohub.org/public/Digitalizer/PA1_04s_cassette/TerComponent/1
component: https://synbiohub.org/public/Digitalizer/PA1_04s_cassette/ProComponent/1
sequence annotation: https://synbiohub.org/public/Digitalizer/PA1_04s_cassette/PA1_04s_cassetteSequenceAnnotation2/1
sequence annotation: https://synbiohub.org/public/Digitalizer/PA1_04s_cassette/PA1_04s_cassetteSequenceAnnotation1/1
sequence annotation: https://synbiohub.org/public/Digitalizer/PA1_04s_cassette/PA1_04s_cassetteSequenceAnnotation/1
sequence constraint: https://synbiohub.org/public/Digitalizer/PA1_04s_cassette/SequenceConstraint1/1
sequence constraint: https://synbiohub.org/public/Digitalizer/PA1_04s_cassette/SequenceConstraint/1

https://synbiohub.org/public/Digitalizer/PA1_04s_cassette/CDSComponent/1
type: Component
persistent identity: CDSComponent
display id: CDSComponent
owned by: Angel
top level: PA1_04s_cassette
access: public
definition: https://synbiohub.org/public/Digitalizer/CDS/1

https://synbiohub.org/public/Digitalizer/PA1_04s_cassette/TerComponent/1
type: Component
persistent identity: TerComponent
display id: TerComponent
owned by: Angel
top level: PA1_04s_cassette
access: public
definition: https://synbiohub.org/public/Digitalizer/Ter/1

https://synbiohub.org/public/Digitalizer/PA1_04s_cassette/ProComponent/1
type: Component
persistent identity: ProComponent
display id: ProComponent
owned by: Angel
top level: PA1_04s_cassette
access: public
definition: PA1_04s

https://synbiohub.org/public/Digitalizer/PA1_04s_cassette/PA1_04s_cassetteSequenceAnnotation2/1
type: SequenceAnnotation
persistent identity: PA1 04s cassetteSequenceAnnotation2
display id: PA1_04s_cassetteSequenceAnnotation2
owned by: Angel
top level: PA1_04s_cassette
location: https://synbiohub.org/public/Digitalizer/PA1_04s_cassette/PA1_04s_cassetteSequenceAnnotation2/GenericLocation/1
component: https://synbiohub.org/public/Digitalizer/PA1_04s_cassette/TerComponent/1

https://synbiohub.org/public/Digitalizer/PA1_04s_cassette/PA1_04s_cassetteSequenceAnnotation2/GenericLocation/1
type: GenericLocation
persistent identity: GenericLocation
display id: GenericLocation
owned by: Angel
top level: PA1_04s_cassette
orientation: inline

https://synbiohub.org/public/Digitalizer/PA1_04s_cassette/PA1_04s_cassetteSequenceAnnotation1/1
type: SequenceAnnotation
persistent identity: PA1 04s cassetteSequenceAnnotation1
display id: PA1_04s_cassetteSequenceAnnotation1
owned by: Angel
top level: PA1_04s_cassette
location: https://synbiohub.org/public/Digitalizer/PA1_04s_cassette/PA1_04s_cassetteSequenceAnnotation1/GenericLocation/1
component: https://synbiohub.org/public/Digitalizer/PA1_04s_cassette/CDSComponent/1

https://synbiohub.org/public/Digitalizer/PA1_04s_cassette/PA1_04s_cassetteSequenceAnnotation1/GenericLocation/1
type: GenericLocation
persistent identity: GenericLocation
display id: GenericLocation
owned by: Angel
top level: PA1_04s_cassette
orientation: inline

https://synbiohub.org/public/Digitalizer/PA1_04s_cassette/PA1_04s_cassetteSequenceAnnotation/1
type: SequenceAnnotation
persistent identity: PA1 04s cassetteSequenceAnnotation
display id: PA1_04s_cassetteSequenceAnnotation
owned by: Angel
top level: PA1_04s_cassette
location: https://synbiohub.org/public/Digitalizer/PA1_04s_cassette/PA1_04s_cassetteSequenceAnnotation/GenericLocation/1
component: https://synbiohub.org/public/Digitalizer/PA1_04s_cassette/ProComponent/1

https://synbiohub.org/public/Digitalizer/PA1_04s_cassette/PA1_04s_cassetteSequenceAnnotation/GenericLocation/1
type: GenericLocation
persistent identity: GenericLocation
display id: GenericLocation
owned by: Angel
top level: PA1_04s_cassette
orientation: inline

https://synbiohub.org/public/Digitalizer/PA1_04s_cassette/SequenceConstraint1/1
type: SequenceConstraint
persistent identity: SequenceConstraint1
display id: SequenceConstraint1
owned by: Angel
top level: PA1_04s_cassette
restriction: precedes
subject: https://synbiohub.org/public/Digitalizer/PA1_04s_cassette/CDSComponent/1
object: https://synbiohub.org/public/Digitalizer/PA1_04s_cassette/TerComponent/1

https://synbiohub.org/public/Digitalizer/PA1_04s_cassette/SequenceConstraint/1
type: SequenceConstraint
persistent identity: SequenceConstraint
display id: SequenceConstraint
owned by: Angel
top level: PA1_04s_cassette
restriction: precedes
subject: https://synbiohub.org/public/Digitalizer/PA1_04s_cassette/ProComponent/1
object: https://synbiohub.org/public/Digitalizer/PA1_04s_cassette/CDSComponent/1

LacI_cassette
type: ComponentDefinition
persistent identity: LacI cassette
display id: LacI_cassette
version: 1
owned by: Angel
owned by: myers
type: DnaRegion
role: SO:0000316
component: https://synbiohub.org/public/Digitalizer/LacI_cassette/CDS1Component/1
component: https://synbiohub.org/public/Digitalizer/LacI_cassette/SpacerComponent/1
component: https://synbiohub.org/public/Digitalizer/LacI_cassette/CouplingComponent/1
sequence annotation: https://synbiohub.org/public/Digitalizer/LacI_cassette/LacI_cassetteSequenceAnnotation/1
sequence annotation: https://synbiohub.org/public/Digitalizer/LacI_cassette/LacI_cassetteSequenceAnnotation2/1
sequence annotation: https://synbiohub.org/public/Digitalizer/LacI_cassette/LacI_cassetteSequenceAnnotation1/1
sequence constraint: https://synbiohub.org/public/Digitalizer/LacI_cassette/SequenceConstraint/1
sequence constraint: https://synbiohub.org/public/Digitalizer/LacI_cassette/SequenceConstraint1/1
sequence: https://synbiohub.org/public/Digitalizer/LacI_cassetteSequence/1

https://synbiohub.org/public/Digitalizer/LacI_cassette/CDS1Component/1
type: Component
persistent identity: CDS1Component
display id: CDS1Component
version: 1
owned by: Angel
owned by: myers
top level: LacI_cassette
access: public
definition: LacI

https://synbiohub.org/public/Digitalizer/LacI_cassette/SpacerComponent/1
type: Component
persistent identity: SpacerComponent
display id: SpacerComponent
version: 1
owned by: Angel
owned by: myers
top level: LacI_cassette
access: public
definition: Spacer

https://synbiohub.org/public/Digitalizer/LacI_cassette/CouplingComponent/1
type: Component
persistent identity: CouplingComponent
display id: CouplingComponent
version: 1
owned by: Angel
owned by: myers
top level: LacI_cassette
access: public
definition: Coupling

https://synbiohub.org/public/Digitalizer/LacI_cassette/LacI_cassetteSequenceAnnotation/1
type: SequenceAnnotation
persistent identity: LacI cassetteSequenceAnnotation
display id: LacI_cassetteSequenceAnnotation
version: 1
owned by: Angel
owned by: myers
top level: LacI_cassette
location: https://synbiohub.org/public/Digitalizer/LacI_cassette/LacI_cassetteSequenceAnnotation/LacI_cassetteSequenceAnnotationRange/1
component: https://synbiohub.org/public/Digitalizer/LacI_cassette/SpacerComponent/1

https://synbiohub.org/public/Digitalizer/LacI_cassette/LacI_cassetteSequenceAnnotation/LacI_cassetteSequenceAnnotationRange/1
type: Range
persistent identity: LacI cassetteSequenceAnnotationRange
display id: LacI_cassetteSequenceAnnotationRange
version: 1
owned by: Angel
owned by: myers
top level: LacI_cassette
start: 1
end: 11
orientation: inline

https://synbiohub.org/public/Digitalizer/LacI_cassette/LacI_cassetteSequenceAnnotation2/1
type: SequenceAnnotation
persistent identity: LacI cassetteSequenceAnnotation2
display id: LacI_cassetteSequenceAnnotation2
version: 1
owned by: Angel
owned by: myers
top level: LacI_cassette
location: https://synbiohub.org/public/Digitalizer/LacI_cassette/LacI_cassetteSequenceAnnotation2/LacI_cassetteSequenceAnnotation2Range/1
component: https://synbiohub.org/public/Digitalizer/LacI_cassette/CouplingComponent/1

https://synbiohub.org/public/Digitalizer/LacI_cassette/LacI_cassetteSequenceAnnotation2/LacI_cassetteSequenceAnnotation2Range/1
type: Range
persistent identity: LacI cassetteSequenceAnnotation2Range
display id: LacI_cassetteSequenceAnnotation2Range
version: 1
owned by: Angel
owned by: myers
top level: LacI_cassette
start: 1092
end: 1128
orientation: inline

https://synbiohub.org/public/Digitalizer/LacI_cassette/LacI_cassetteSequenceAnnotation1/1
type: SequenceAnnotation
persistent identity: LacI cassetteSequenceAnnotation1
display id: LacI_cassetteSequenceAnnotation1
version: 1
owned by: Angel
owned by: myers
top level: LacI_cassette
location: https://synbiohub.org/public/Digitalizer/LacI_cassette/LacI_cassetteSequenceAnnotation1/LacI_cassetteSequenceAnnotation1Range/1
component: https://synbiohub.org/public/Digitalizer/LacI_cassette/CDS1Component/1

https://synbiohub.org/public/Digitalizer/LacI_cassette/LacI_cassetteSequenceAnnotation1/LacI_cassetteSequenceAnnotation1Range/1
type: Range
persistent identity: LacI cassetteSequenceAnnotation1Range
display id: LacI_cassetteSequenceAnnotation1Range
version: 1
owned by: Angel
owned by: myers
top level: LacI_cassette
start: 12
end: 1091
orientation: inline

https://synbiohub.org/public/Digitalizer/LacI_cassette/SequenceConstraint/1
type: SequenceConstraint
persistent identity: SequenceConstraint
display id: SequenceConstraint
version: 1
owned by: Angel
owned by: myers
top level: LacI_cassette
restriction: precedes
subject: https://synbiohub.org/public/Digitalizer/LacI_cassette/SpacerComponent/1
object: https://synbiohub.org/public/Digitalizer/LacI_cassette/CDS1Component/1

https://synbiohub.org/public/Digitalizer/LacI_cassette/SequenceConstraint1/1
type: SequenceConstraint
persistent identity: SequenceConstraint1
display id: SequenceConstraint1
version: 1
owned by: Angel
owned by: myers
top level: LacI_cassette
restriction: precedes
subject: https://synbiohub.org/public/Digitalizer/LacI_cassette/CDS1Component/1
object: https://synbiohub.org/public/Digitalizer/LacI_cassette/CouplingComponent/1

ATG
type: ComponentDefinition
persistent identity: ATG
display id: ATG
version: 1
owned by: Angel
owned by: myers
type: DnaRegion
role: SO:0000804
sequence: https://synbiohub.org/public/Digitalizer/ATGSequence/1

T1_TE
type: ComponentDefinition
persistent identity: T1 TE
display id: T1_TE
version: 1
owned by: Angel
owned by: myers
type: DnaRegion
role: SO:0000141
sequence: https://synbiohub.org/public/Digitalizer/T1_TESequence/1

T500
type: ComponentDefinition
persistent identity: T500
display id: T500
version: 1
owned by: Angel
owned by: myers
type: DnaRegion
role: SO:0000141
sequence: https://synbiohub.org/public/Digitalizer/T500Sequence/1

XylSPm
type: ComponentDefinition
persistent identity: XylSPm
display id: XylSPm
version: 1
owned by: Angel
owned by: myers
type: DnaRegion
role: SO:0000167
sequence: https://synbiohub.org/public/Digitalizer/XylSPmSequence/1

EcoRI
type: ComponentDefinition
persistent identity: EcoRI
display id: EcoRI
version: 1
owned by: Angel
owned by: myers
type: DnaRegion
role: SO:0001692
sequence: https://synbiohub.org/public/Digitalizer/EcoRISequence/1

NheI
type: ComponentDefinition
persistent identity: NheI
display id: NheI
version: 1
owned by: Angel
owned by: myers
type: DnaRegion
role: SO:0001692
sequence: https://synbiohub.org/public/Digitalizer/NheISequence/1

MCS
type: ComponentDefinition
persistent identity: MCS
display id: MCS
version: 1
owned by: Angel
owned by: myers
type: DnaRegion
role: SO:0000804
component: https://synbiohub.org/public/Digitalizer/MCS/Restriction_sitesComponent/1
component: https://synbiohub.org/public/Digitalizer/MCS/PBSComponent/1
sequence annotation: https://synbiohub.org/public/Digitalizer/MCS/MCSSequenceAnnotation/1
sequence annotation: https://synbiohub.org/public/Digitalizer/MCS/MCSSequenceAnnotation1/1
sequence constraint: https://synbiohub.org/public/Digitalizer/MCS/SequenceConstraint/1
sequence: https://synbiohub.org/public/Digitalizer/MCSSequence/1

https://synbiohub.org/public/Digitalizer/MCS/Restriction_sitesComponent/1
type: Component
persistent identity: Restriction sitesComponent
display id: Restriction_sitesComponent
version: 1
owned by: Angel
owned by: myers
top level: MCS
access: public
definition: Restriction_sites

https://synbiohub.org/public/Digitalizer/MCS/PBSComponent/1
type: Component
persistent identity: PBSComponent
display id: PBSComponent
version: 1
owned by: Angel
owned by: myers
top level: MCS
access: public
definition: PBS_F24

https://synbiohub.org/public/Digitalizer/MCS/MCSSequenceAnnotation/1
type: SequenceAnnotation
persistent identity: MCSSequenceAnnotation
display id: MCSSequenceAnnotation
version: 1
owned by: Angel
owned by: myers
top level: MCS
location: https://synbiohub.org/public/Digitalizer/MCS/MCSSequenceAnnotation/MCSSequenceAnnotationRange/1
component: https://synbiohub.org/public/Digitalizer/MCS/Restriction_sitesComponent/1

https://synbiohub.org/public/Digitalizer/MCS/MCSSequenceAnnotation/MCSSequenceAnnotationRange/1
type: Range
persistent identity: MCSSequenceAnnotationRange
display id: MCSSequenceAnnotationRange
version: 1
owned by: Angel
owned by: myers
top level: MCS
start: 1
end: 59
orientation: inline

https://synbiohub.org/public/Digitalizer/MCS/MCSSequenceAnnotation1/1
type: SequenceAnnotation
persistent identity: MCSSequenceAnnotation1
display id: MCSSequenceAnnotation1
version: 1
owned by: Angel
owned by: myers
top level: MCS
location: https://synbiohub.org/public/Digitalizer/MCS/MCSSequenceAnnotation1/MCSSequenceAnnotation1Range/1
component: https://synbiohub.org/public/Digitalizer/MCS/PBSComponent/1

https://synbiohub.org/public/Digitalizer/MCS/MCSSequenceAnnotation1/MCSSequenceAnnotation1Range/1
type: Range
persistent identity: MCSSequenceAnnotation1Range
display id: MCSSequenceAnnotation1Range
version: 1
owned by: Angel
owned by: myers
top level: MCS
start: 60
end: 83
orientation: inline

https://synbiohub.org/public/Digitalizer/MCS/SequenceConstraint/1
type: SequenceConstraint
persistent identity: SequenceConstraint
display id: SequenceConstraint
version: 1
owned by: Angel
owned by: myers
top level: MCS
restriction: precedes
subject: https://synbiohub.org/public/Digitalizer/MCS/Restriction_sitesComponent/1
object: https://synbiohub.org/public/Digitalizer/MCS/PBSComponent/1

https://synbiohub.org/public/Digitalizer/SpacerSequence/1
type: Sequence
persistent identity: SpacerSequence
display id: SpacerSequence
version: 1
owned by: Angel
owned by: myers
elements: aaaaacatatg
encoding: naseq.html

https://synbiohub.org/public/Digitalizer/LacI_cassetteSequence/1
type: Sequence
persistent identity: LacI cassetteSequence
display id: LacI_cassetteSequence
version: 1
owned by: Angel
owned by: myers
elements: aaaaacatatggtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagcatcatcaccaccatcattaggatggtggtgatgata
encoding: naseq.html

https://synbiohub.org/public/Digitalizer/all_or_nothing_PA1_04sSequence/1
type: Sequence
persistent identity: all or nothing PA1 04sSequence
display id: all_or_nothing_PA1_04sSequence
version: 1
owned by: Angel
owned by: myers
elements: tttatcaaaaagagtgttgacttgtgagcgctcacattgatacttagattcagtataacgttactggtttcaccattttctgttgggccattgcattgccactgattttccaacatataaaaagacaagcccgaacagtcgtccgggctttttttctcgagctcgagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgtttttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/Digitalizer/LacISequence/1
type: Sequence
persistent identity: LacISequence
display id: LacISequence
version: 1
owned by: Angel
owned by: myers
elements: gtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcag
encoding: naseq.html

https://synbiohub.org/public/Digitalizer/RBS_cassetteSequence/1
type: Sequence
persistent identity: RBS cassetteSequence
display id: RBS_cassetteSequence
version: 1
owned by: Angel
owned by: myers
elements: cctaggagga
encoding: naseq.html

https://synbiohub.org/public/Digitalizer/PBS_F24Sequence/1
type: Sequence
persistent identity: PBS F24Sequence
display id: PBS_F24Sequence
version: 1
owned by: Angel
owned by: myers
elements: gtcgtgactgggaaaaccctggcg
encoding: naseq.html

https://synbiohub.org/public/Digitalizer/PacISequence1/1
type: Sequence
persistent identity: PacISequence1
display id: PacISequence1
version: 1
owned by: Angel
owned by: myers
elements: ttaattaa
encoding: naseq.html

https://synbiohub.org/public/Digitalizer/all_or_nothingSequence/1
type: Sequence
persistent identity: all or nothingSequence
display id: all_or_nothingSequence
version: 1
owned by: Angel
owned by: myers
elements: ttaattaaacgttcgtaatcaagccacttcctttttgcattgacgcagggtgtcggaaggcaactcgccgaacgcgctcctatagttttcagcgaagcgtcccaaatgtaagaagccgtagtctagggctatctcagttatactacgcacattggcactgggatcgttcaagcaggcgcggatgctttcgagcttgcggttgcggatgtagttcttcggcgtggtgccggcgtgcttctcgaacaaattgtagagcgagcgtggactcatcatcgccagctccgctaaccgctcaaggctgatattccgtttgagattctcctcaatgaattgaacgactcgctcgaaagacgggttacctttgctgaaaatttcacggctgacattgctgcccagcatttcgagcagcttggaagcgatgatccccgcatagtgctcttggacccgaggcatcgactttgtatgttccgcttcgtcacaaactaacccgagtagattgataaagccatcgagttgctggagattgtgtcgcgcggcgaaacggataccctccctcggcttgtgccaattgttgtcactgcacgcccgatcaaggaccactgagggcaatttaacgataaatttctcgcaatcttctgaataggtcaggtcggcttggtcatccggattgagcagcaatagttcgcccggcgcaaaatagtgctcctggccatggccacgccacaggcaatggcctttgagtattatttgcagatgataacaggtctctaatccaggcgagattaccctcacgctaccgccgtagctgattcgacacagatcgaggcatccgaagattctgtggtgcagcctgcctgccgggcgcccgcccttgggcaggcgaatagagtgcgtaccgacatactggttaacataatcggagactgcatagggctcggcgtggacgaagatctgacttttctcgttcaataagcaaaaatccatagttcacggttctcttattttaatgtgggctgcttggtgtgatgtagaaaggcgccaagtcgatgaaaatgcatctcgacgtgatgcgtatacgggttacccccattgccacgttgcgccatcctttttgcaatcagtgaccacttttccaagcaaaaataacgccaagcagaacgaagacgttctttttaagaagcgagaacaccagaagttcgtgctgtcggggcatggggcgacgaattggcggataaaggggatctgctggatattacggcctttttaaagaccgtaaagaaaaataagcacaagttttatccggcctttattcacattcttgcccgcctgatgaatgctcatccgtaattacgtatggcaatgaaagacggtgagctggtgatatgggatagtgttcacccttgttacaccgttttccatgagcaaactgaaacgttttcatcgctctggagtgaataccacgacgatttccggcagtttctacacatatattcgcaagatgtggcgtgttacggtgaaaacctggcctatttccctaaagggtttattgagaatatgtttttcgtctcagccaatccctgggtgagtttcaccagttttgatttaaacgtggccaatatggacaacttcttcgcccccgttttcaccatgcatgggaattagcttgatctgaccaacgaccggtagcggagctatccaacggcggtataccaggaaaacacacagcaggtacatcagaacagtaccatgactgaagaacaaatagttttttcctgatccataaagcagaacggcctgctccatgacaaatctggctccccaactaatgccccatgcagccagcataaccagcataaagtgcagtgtccggtttgatagggataagtccagccttgcaagaagcggatacaggagtgcaaaaaatggctatctctagtaaggcctaccccttaggctttatgcaacagaaacaataataatggagtcatgaccatgcctag…
encoding: naseq.html

https://synbiohub.org/public/Digitalizer/sRNA_cassetteSequence/1
type: Sequence
persistent identity: sRNA cassetteSequence
display id: sRNA_cassetteSequence
version: 1
owned by: Angel
owned by: myers
elements: tataacgttactggtttcaccattttctgttgggccattgcattgccactgattttccaacatataaaaagacaagcccgaacagtcgtccgggctttttttctcgag
encoding: naseq.html

https://synbiohub.org/public/Digitalizer/MICc_scaffoldSequence/1
type: Sequence
persistent identity: MICc scaffoldSequence
display id: MICc_scaffoldSequence
version: 1
owned by: Angel
owned by: myers
elements: tttctgttgggccattgcattgccactgattttccaacatataaaaagacaagcccgaacagtcgtccgggctttttttctcgag
encoding: naseq.html

https://synbiohub.org/public/Digitalizer/NheISequence/1
type: Sequence
persistent identity: NheISequence
display id: NheISequence
version: 1
owned by: Angel
owned by: myers
elements: gctagc
encoding: naseq.html

https://synbiohub.org/public/Digitalizer/EcoRISequence/1
type: Sequence
persistent identity: EcoRISequence
display id: EcoRISequence
version: 1
owned by: Angel
owned by: myers
elements: gaattc
encoding: naseq.html

https://synbiohub.org/public/Digitalizer/T1_TESequence/1
type: Sequence
persistent identity: T1 TESequence
display id: T1_TESequence
version: 1
owned by: Angel
owned by: myers
elements: ctcgagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgtttttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/Digitalizer/sRNA_targetSequence/1
type: Sequence
persistent identity: sRNA targetSequence
display id: sRNA_targetSequence
version: 1
owned by: Angel
owned by: myers
elements: tataacgttactggtttcaccat
encoding: naseq.html

https://synbiohub.org/public/Digitalizer/PA1_04sSequence/1
type: Sequence
persistent identity: PA1 04sSequence
display id: PA1_04sSequence
version: 1
owned by: Angel
owned by: myers
elements: tttatcaaaaagagtgttgacttgtgagcgctcacattgatacttagattcag
encoding: naseq.html

https://synbiohub.org/public/Digitalizer/gfp_cassetteSequence/1
type: Sequence
persistent identity: gfp cassetteSequence
display id: gfp_cassetteSequence
version: 1
owned by: Angel
owned by: myers
elements: atggctagccgtaaaggtgaagaactgttcaccggtgttgttccgatcctggttgaactggatggtgatgttaacggccacaaattctctgttcgtggtgaaggtgaaggtgatgcaaccaacggtaaactgaccctgaaattcatctgcactaccggtaaactgccggttccatggccgactctggtgactaccctgacctatggtgttcagtgtttttctcgttacccggatcacatgaagcagcatgatttcttcaaatctgcaatgccggaaggttatgtacaggagcgcaccatttctttcaaagacgatggcacctacaaaacccgtgcagaggttaaatttgaaggtgatactctggtgaaccgtattgaactgaaaggcattgatttcaaagaggacggcaacatcctgggccacaaactggaatataacttcaactcccataacgtttacatcaccgcagacaaacagaagaacggtatcaaagctaacttcaaaattcgccataacgttgaagacggtagcgtacagctggcggaccactaccagcagaacactccgatcggtgatggtccggttctgctgccggataaccactacctgtccacccagtctaaactgtccaaagacccgaacgaaaagcgcgaccacatggtgctgctggagttcgttactgccgcaggtatcacgcacggcatggatgaactctacaaataagaattc
encoding: naseq.html

https://synbiohub.org/public/Digitalizer/msfGFPSequence/1
type: Sequence
persistent identity: msfGFPSequence
display id: msfGFPSequence
version: 1
owned by: Angel
owned by: myers
elements: cgtaaaggtgaagaactgttcaccggtgttgttccgatcctggttgaactggatggtgatgttaacggccacaaattctctgttcgtggtgaaggtgaaggtgatgcaaccaacggtaaactgaccctgaaattcatctgcactaccggtaaactgccggttccatggccgactctggtgactaccctgacctatggtgttcagtgtttttctcgttacccggatcacatgaagcagcatgatttcttcaaatctgcaatgccggaaggttatgtacaggagcgcaccatttctttcaaagacgatggcacctacaaaacccgtgcagaggttaaatttgaaggtgatactctggtgaaccgtattgaactgaaaggcattgatttcaaagaggacggcaacatcctgggccacaaactggaatataacttcaactcccataacgtttacatcaccgcagacaaacagaagaacggtatcaaagctaacttcaaaattcgccataacgttgaagacggtagcgtacagctggcggaccactaccagcagaacactccgatcggtgatggtccggttctgctgccggataaccactacctgtccacccagtctaaactgtccaaagacccgaacgaaaagcgcgaccacatggtgctgctggagttcgttactgccgcaggtatcacgcacggcatggatgaactctacaaataa
encoding: naseq.html

https://synbiohub.org/public/Digitalizer/ATGSequence/1
type: Sequence
persistent identity: ATGSequence
display id: ATGSequence
version: 1
owned by: Angel
owned by: myers
elements: atg
encoding: naseq.html

https://synbiohub.org/public/Digitalizer/CouplingSequence/1
type: Sequence
persistent identity: CouplingSequence
display id: CouplingSequence
version: 1
owned by: Angel
owned by: myers
elements: catcatcaccaccatcattaggatggtggtgatgata
encoding: naseq.html

https://synbiohub.org/public/Digitalizer/XylSPmSequence/1
type: Sequence
persistent identity: XylSPmSequence
display id: XylSPmSequence
version: 1
owned by: Angel
owned by: myers
elements: acgttcgtaatcaagccacttcctttttgcattgacgcagggtgtcggaaggcaactcgccgaacgcgctcctatagttttcagcgaagcgtcccaaatgtaagaagccgtagtctagggctatctcagttatactacgcacattggcactgggatcgttcaagcaggcgcggatgctttcgagcttgcggttgcggatgtagttcttcggcgtggtgccggcgtgcttctcgaacaaattgtagagcgagcgtggactcatcatcgccagctccgctaaccgctcaaggctgatattccgtttgagattctcctcaatgaattgaacgactcgctcgaaagacgggttacctttgctgaaaatttcacggctgacattgctgcccagcatttcgagcagcttggaagcgatgatccccgcatagtgctcttggacccgaggcatcgactttgtatgttccgcttcgtcacaaactaacccgagtagattgataaagccatcgagttgctggagattgtgtcgcgcggcgaaacggataccctccctcggcttgtgccaattgttgtcactgcacgcccgatcaaggaccactgagggcaatttaacgataaatttctcgcaatcttctgaataggtcaggtcggcttggtcatccggattgagcagcaatagttcgcccggcgcaaaatagtgctcctggccatggccacgccacaggcaatggcctttgagtattatttgcagatgataacaggtctctaatccaggcgagattaccctcacgctaccgccgtagctgattcgacacagatcgaggcatccgaagattctgtggtgcagcctgcctgccgggcgcccgcccttgggcaggcgaatagagtgcgtaccgacatactggttaacataatcggagactgcatagggctcggcgtggacgaagatctgacttttctcgttcaataagcaaaaatccatagttcacggttctcttattttaatgtgggctgcttggtgtgatgtagaaaggcgccaagtcgatgaaaatgcatctcgacgtgatgcgtatacgggttacccccattgccacgttgcgccatcctttttgcaatcagtgaccacttttccaagcaaaaataacgccaagcagaacgaagacgttctttttaagaagcgagaacaccagaagttcgtgctgtcggggcatggggcgacgaattggcggataaaggggatctgctggatattacggcctttttaaagaccgtaaagaaaaataagcacaagttttatccggcctttattcacattcttgcccgcctgatgaatgctcatccgtaattacgtatggcaatgaaagacggtgagctggtgatatgggatagtgttcacccttgttacaccgttttccatgagcaaactgaaacgttttcatcgctctggagtgaataccacgacgatttccggcagtttctacacatatattcgcaagatgtggcgtgttacggtgaaaacctggcctatttccctaaagggtttattgagaatatgtttttcgtctcagccaatccctgggtgagtttcaccagttttgatttaaacgtggccaatatggacaacttcttcgcccccgttttcaccatgcatgggaattagcttgatctgaccaacgaccggtagcggagctatccaacggcggtataccaggaaaacacacagcaggtacatcagaacagtaccatgactgaagaacaaatagttttttcctgatccataaagcagaacggcctgctccatgacaaatctggctccccaactaatgccccatgcagccagcataaccagcataaagtgcagtgtccggtttgatagggataagtccagccttgcaagaagcggatacaggagtgcaaaaaatggctatctctagtaaggcctaccccttaggctttatgcaacagaaacaataataatggagtcatgaccatg
encoding: naseq.html

https://synbiohub.org/public/Digitalizer/MCSSequence/1
type: Sequence
persistent identity: MCSSequence
display id: MCSSequence
version: 1
owned by: Angel
owned by: myers
elements: gagctcggtacccggggatcctctagagtcgacctgcaggcatgcaagcttgcggccgcgtcgtgactgggaaaaccctggcg
encoding: naseq.html

https://synbiohub.org/public/Digitalizer/Restriction_sitesSequence/1
type: Sequence
persistent identity: Restriction sitesSequence
display id: Restriction_sitesSequence
version: 1
owned by: Angel
owned by: myers
elements: gagctcggtacccggggatcctctagagtcgacctgcaggcatgcaagcttgcggccgc
encoding: naseq.html

https://synbiohub.org/public/Digitalizer/all_or_nothing_PmSequence/1
type: Sequence
persistent identity: all or nothing PmSequence
display id: all_or_nothing_PmSequence
version: 1
owned by: Angel
owned by: myers
elements: acgttcgtaatcaagccacttcctttttgcattgacgcagggtgtcggaaggcaactcgccgaacgcgctcctatagttttcagcgaagcgtcccaaatgtaagaagccgtagtctagggctatctcagttatactacgcacattggcactgggatcgttcaagcaggcgcggatgctttcgagcttgcggttgcggatgtagttcttcggcgtggtgccggcgtgcttctcgaacaaattgtagagcgagcgtggactcatcatcgccagctccgctaaccgctcaaggctgatattccgtttgagattctcctcaatgaattgaacgactcgctcgaaagacgggttacctttgctgaaaatttcacggctgacattgctgcccagcatttcgagcagcttggaagcgatgatccccgcatagtgctcttggacccgaggcatcgactttgtatgttccgcttcgtcacaaactaacccgagtagattgataaagccatcgagttgctggagattgtgtcgcgcggcgaaacggataccctccctcggcttgtgccaattgttgtcactgcacgcccgatcaaggaccactgagggcaatttaacgataaatttctcgcaatcttctgaataggtcaggtcggcttggtcatccggattgagcagcaatagttcgcccggcgcaaaatagtgctcctggccatggccacgccacaggcaatggcctttgagtattatttgcagatgataacaggtctctaatccaggcgagattaccctcacgctaccgccgtagctgattcgacacagatcgaggcatccgaagattctgtggtgcagcctgcctgccgggcgcccgcccttgggcaggcgaatagagtgcgtaccgacatactggttaacataatcggagactgcatagggctcggcgtggacgaagatctgacttttctcgttcaataagcaaaaatccatagttcacggttctcttattttaatgtgggctgcttggtgtgatgtagaaaggcgccaagtcgatgaaaatgcatctcgacgtgatgcgtatacgggttacccccattgccacgttgcgccatcctttttgcaatcagtgaccacttttccaagcaaaaataacgccaagcagaacgaagacgttctttttaagaagcgagaacaccagaagttcgtgctgtcggggcatggggcgacgaattggcggataaaggggatctgctggatattacggcctttttaaagaccgtaaagaaaaataagcacaagttttatccggcctttattcacattcttgcccgcctgatgaatgctcatccgtaattacgtatggcaatgaaagacggtgagctggtgatatgggatagtgttcacccttgttacaccgttttccatgagcaaactgaaacgttttcatcgctctggagtgaataccacgacgatttccggcagtttctacacatatattcgcaagatgtggcgtgttacggtgaaaacctggcctatttccctaaagggtttattgagaatatgtttttcgtctcagccaatccctgggtgagtttcaccagttttgatttaaacgtggccaatatggacaacttcttcgcccccgttttcaccatgcatgggaattagcttgatctgaccaacgaccggtagcggagctatccaacggcggtataccaggaaaacacacagcaggtacatcagaacagtaccatgactgaagaacaaatagttttttcctgatccataaagcagaacggcctgctccatgacaaatctggctccccaactaatgccccatgcagccagcataaccagcataaagtgcagtgtccggtttgatagggataagtccagccttgcaagaagcggatacaggagtgcaaaaaatggctatctctagtaaggcctaccccttaggctttatgcaacagaaacaataataatggagtcatgaccatgcctaggaggaaaa…
encoding: naseq.html

https://synbiohub.org/public/Digitalizer/SpeISequence/1
type: Sequence
persistent identity: SpeISequence
display id: SpeISequence
version: 1
owned by: Angel
owned by: myers
elements: actagt
encoding: naseq.html

https://synbiohub.org/public/Digitalizer/T500Sequence/1
type: Sequence
persistent identity: T500Sequence
display id: T500Sequence
version: 1
owned by: Angel
owned by: myers
elements: caaagcccgccgaaaggcgggcttttctgt
encoding: naseq.html