##gff-version 3
##sequence-region g531_big_boy_cir_synthetic_circular_DNA_comp 172 7861
g531_big_boy_cir_synthetic_circular_DNA_comp	.	sequence_feature	3759	3833	.	+	0	ID=RiboJ71;Name=RiboJ71
g531_big_boy_cir_synthetic_circular_DNA_comp	.	CDS	4748	5047	.	+	0	ID=jr9;Name=jr9
g531_big_boy_cir_synthetic_circular_DNA_comp	.	CDS	5328	6005	.	+	0	ID=rfp;Name=rfp
g531_big_boy_cir_synthetic_circular_DNA_comp	.	promoter	4583	4650	.	+	0	ID=PJR4;Name=PJR4
g531_big_boy_cir_synthetic_circular_DNA_comp	.	sequence_feature	6220	6669	.	+	0	ID=R6K;Name=R6K
g531_big_boy_cir_synthetic_circular_DNA_comp	.	terminator	5048	5150	.	+	0	ID=DT86;Name=DT86
g531_big_boy_cir_synthetic_circular_DNA_comp	.	promoter	3062	3141	.	+	0	ID=PJR3b;Name=PJR3b
g531_big_boy_cir_synthetic_circular_DNA_comp	.	terminator	2968	3057	.	+	0	ID=ECK120029600;Name=ECK120029600
g531_big_boy_cir_synthetic_circular_DNA_comp	.	terminator	4494	4582	.	+	0	ID=ilvGEDA;Name=ilvGEDA
g531_big_boy_cir_synthetic_circular_DNA_comp	.	sequence_feature	3146	3224	.	+	0	ID=RiboJ60;Name=RiboJ60
g531_big_boy_cir_synthetic_circular_DNA_comp	.	terminator	6018	6061	.	+	0	ID=ECK120017009;Name=ECK120017009
g531_big_boy_cir_synthetic_circular_DNA_comp	.	sequence_feature	4651	4725	.	+	0	ID=RiboJ64;Name=RiboJ64
g531_big_boy_cir_synthetic_circular_DNA_comp	.	CDS	257	976	.	-	0	ID=yfp;Name=yfp
g531_big_boy_cir_synthetic_circular_DNA_comp	.	CDS	3249	3548	.	+	0	ID=jr8;Name=jr8
g531_big_boy_cir_synthetic_circular_DNA_comp	.	terminator	172	256	.	-	0	ID=DT65;Name=DT65
g531_big_boy_cir_synthetic_circular_DNA_comp	.	promoter	5155	5222	.	+	0	ID=PJR7;Name=PJR7
g531_big_boy_cir_synthetic_circular_DNA_comp	.	CDS	3858	4493	.	+	0	ID=jr2;Name=jr2
g531_big_boy_cir_synthetic_circular_DNA_comp	.	sequence_feature	1218	1292	.	+	0	ID=RiboJ69;Name=RiboJ69
g531_big_boy_cir_synthetic_circular_DNA_comp	.	sequence_feature	6066	6128	.	+	0	ID=attP2;Name=attP2
g531_big_boy_cir_synthetic_circular_DNA_comp	.	terminator	3549	3682	.	+	0	ID=DT56;Name=DT56
g531_big_boy_cir_synthetic_circular_DNA_comp	.	sequence_feature	6675	6934	.	+	0	ID=oriT;Name=oriT
g531_big_boy_cir_synthetic_circular_DNA_comp	.	CDS	2131	2967	.	+	0	ID=jr4;Name=jr4
g531_big_boy_cir_synthetic_circular_DNA_comp	.	terminator	1858	1940	.	+	0	ID=rpoC;Name=rpoC
g531_big_boy_cir_synthetic_circular_DNA_comp	.	promoter	1074	1135	.	-	0	ID=PJR1;Name=PJR1
g531_big_boy_cir_synthetic_circular_DNA_comp	.	promoter	1945	2034	.	+	0	ID=PCymRC;Name=PCymRC
g531_big_boy_cir_synthetic_circular_DNA_comp	.	sequence_feature	2035	2110	.	+	0	ID=BydvJ;Name=BydvJ
g531_big_boy_cir_synthetic_circular_DNA_comp	.	promoter	1140	1213	.	+	0	ID=Ptet;Name=Ptet
g531_big_boy_cir_synthetic_circular_DNA_comp	.	promoter	3687	3754	.	+	0	ID=PJR4;Name=PJR4
g531_big_boy_cir_synthetic_circular_DNA_comp	.	CDS	7046	7861	.	-	0	ID=kanR;Name=kanR
g531_big_boy_cir_synthetic_circular_DNA_comp	.	CDS	1315	1857	.	+	0	ID=jr3;Name=jr3
>g531_big_boy_cir_synthetic_circular_DNA_comp
TAGGCATGGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGAAGTTCCTATTCCGAAGTTCCTATTCT
CTAGAAAGTATAGGAACTTCGGATCCGAATTCGAGCTCCGTCGACACTAGTGCGGCCGCACTCGAGGGCATCAGAGCAG
ATTGTACTGGTACGCGGAGAAAAGGTCCGAAAATTCGGACCCGATGGAATTGGATAAAAAAAGGCCCCTGTTGAAATTG
CAGGGGCCTGGTACGAGCATTACTTGTACAGCTCGTCCATGCCGAGAGTGATCCCGGCGGCGGTCACGAACTCCAGCAG
GACCATGTGATCGCGCTTCTCGTTGGGGTCTTTGCTCAGGGCGGACTGGTAGCTAAGGTAGTGGTTGTCGGGCAGCAGC
ACGGGGCCGTCGCCGATTGGGGTGTTCTGCTGGTAGTGGTCGGCGAGCTGCACGCTGCCGTCCTCGATGTTGTGGCGGA
TCTTGAAGTTCACCTTGATGCCGTTCTTCTGCTTGTCGGCCATGATATAGACGTTGTGGCTGTTGTAGTTGTACTCCAG
CTTGTGCCCCAGGATGTTGCCGTCCTCCTTGAAGTCGATGCCCTTCAGCTCGATGCGGTTCACCAGGGTGTCGCCCTCG
AACTTCACCTCGGCGCGGGTCTTGTAGTTGCCGTCGTCCTTGAAGAAGATGGTGCGCTCCTGGACGTAGCCTTCGGGCA
TGGCGGACTTGAAGAAGTCGTGCAGCTTCATGTGGTCGGGGTAGCGGGCGAAGCATTGCAGGCCGTAGCCGAAGGTGGT
CACGAGGGTGGGCCAGGGCACGGGCAGCTTGCCTGTGGTGCAGATGAACTTCAGGGTCAGCTTGCCGTAGGTGGCATCG
CCCTCGCCCTCGCCGGACACGCTGAACTTGTGGCCGTTTACGTCGCCGTCCAGCTCGACCAGGATGGGCACCACCCCGG
TGAACAGCTCCTCGCCCTTGCTCACCATCTAGTATTTCTCCTCTTTCTCTAGTATTAAACAAAATTATTTGTAGAGGCT
GTTTCGTCCTCACGGACTCATCAGACCGGAAAGCACATCCGGTGACAGCTATTATCACCGCCAGAGGTAAAATAGTCAA
CACGCACGGTGTTACCCGGCCTGAAAGAAAAGTTACTCCACCGTTGGCTTTTTTCCCTATCAGTGATAGAGATTGACAT
CCCTATCAGTGATAGAGATAATGAGCACTCAAAGCTGTCAAGGCGTGTGCTTCGCCTTCTGATGAGCCCGTGAGGGCGA
AACAGCCTCTACAAATAATTTTGTTTAAGCTGGTACTGCTCGAGTATCTTATGAAGACTGACACATCAAATCGCTTAAA
GCAGATCATGGCTGAGCGCAACCTTAAACAAGTGGATATCCTGAATCTTAGCATCCCTTTTCAAAAAAAATTCGGGATC
AAATTGAGCAAGTCCACCCTTTCGCAATACGTCAACTCGGTACAATCCCCAGACCAGAACCGTATTTACCTGTTAGCCA
AGACCTTGGGGGTAAGTGAAGCATGGTTGATGGGATTCGACGTTCCAATGGTGGAATCCTCGAAGATTGAGAATGATTC
AGAGAACATTGAGGAGACGATCACCGTCATGAAGAAGCTGGAAGAGCCGCGTCAGAAAGTGGTCTTGGATACAGCAAAA
ATTCAGTTAAAGGAGCAGGATGAACAGAATAAGGTAAAGCAGATCGAGGACTATCGCTTATCCGACGAATATTTGGAGG
AGCAGATCAGCAAGGCGTCGGCTTACGGAGGGGGGCAACTTAACGATAATGATAAGGAATTTTTCAAACGTCTGCTGAA
AAACACCCTTAAGGAGAAAATTGACAAAGGCGATCTTTGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCG
GCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAGGAAAACAAACAGACAATCTGGTCTGTTTGTATTA
TGGAAAATTTTTCTGTATAATAGATTCAACAAACAGACAATCTGGTCTGTTTGTATTATAGGGTGTCTCAAGGTGCGTA
CCTTGACTGATGAGTCCGAAAGGACGAAACACCCCTCTACAAATAATTTTGTTTAATATTTTTAATAAATAGGGTTATG
AAGAAGATTCGCCTTCCCGAAATGATTGACTACTTCCGTAAGGAAAACGGCTGGACTATGAAAGAGTTTGGTGAAAAAT
TAGGGAAGAGTGAGAGCGCTATTAGCAAGTGGATCAAGGGGGTACGCTCCCCTATGGTAGAGGATTTTGACAAGATGGT
GAATTTATTTAACACTGACCCAGAAACCCTGATGTATGGTGCGTCAGATTTATCTACGACGTTGAGCGAAATCAATAAG
ATTTCGAGCCAACTTGAGGAACCGCGCCAGAAGGTTGTACTGAATACGGCAAATAATCAGCTTGACGAACAAAATCAGG
AAAAGAAAAAGGAATCTAAAGTCATCCCTATTAACAAAATCCCAGATGACTTACCACCCTACATTAGTCGCAAAATTCT
GGAAAATTTTGTAATGCCGACTAACACTATGGAATACGAAGCTGATGAAGATATGGTGGACGTACCCATCCTTGGGCGC
ATCGCGGCGGGCCTTCCGCTTGACGCGGTAGAAAACTTTGATGGAACGCGCCCAGTCCCTGCACATTTCCTTTCGAGTG
CCCGTGATTACTATTGGCTTATGGTGGACGGCCATTCTATGGAACCTAAGATTCCGTACGGAGCATACGTCTTGATCGA
AGCCGTCCCCGATGTGAGCGATGGGACCATTGGTGCCGTCTTATTCCATGATGATTGTCAGGCGACGCTGAAAAAGGTA
TATCACGAGATCGACTGTTTGCGCTTAGTCTCAATTAACAAAGAATTTAAGGACCAATTCGCCACGCAAGATAATCCAG
CTGCAGTGATTGGTCAAGCAGTTAAGGTTGAAATCGACTTGTAATTCAGCCAAAAAACTTAAGACCGCCGGTCTTGTCC
ACTACCTTGCAGTAATGCGGTGGACAGGATCGGCGGTTTTCTTTTCTCTTCTCAAAGGAGCCACAAGCTCGAAAAGTTC
ATGAAACGTGAACTTGACAGCTAGCTCAGTCCTAGGTATAAAGTTCATGAAACGTGAACTCCACCTGAAGTCGTCAAGT
GCTGTGCTTGCACTTCTGATGAGGCAGTGATGCCGAAACGACCTCTACAAATAATTTTGTTTAATTATCTTAGGTACAG
GGCACATAAATGTCAATAGACGTTTCGGAGAAGTTGAAGCTAATCCGTGAATCTGAAAGGTTAAACCGTAAAGAATTCA
GTGAATTAACTGGTGTAGCCTACAGCTCACTTTCGAGCTATGAGAGCCGGTCAAAAAACGCTGGAGTTGAAGCCATAAT
GAAGGTCTTACAACATCCTAGATTTACTAAATATACTTTGTGGTTCATGACTGATCAGGTAGCTCCAGAAGCCGGGCAA
ATTGCGCCCGCTCTCGCACACTTTGGGCAAAACGAAACAACGTCGCCCCACTCCGGTCAAAAGACTGGTTAATACCACC
GTCAAAAAAAACGGCGCTTTTTAGCGCCGTTTTTATTTTTCAACCTTCCAGGCATCAAATAAAACGAAAGGCTCAGTCG
AAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCAACCGAGAGCTAACTTTATAACTTGACAAAT
TGGATAGTTTTAGGTATAATAACTTGACAAATTGGATAGTTGCCCAGTTGTCAGCCACTGTGCTTGTGGCTCTGATGAG
ACGGTGACGTCGAAACAACCTCTACAAATAATTTTGTTTAAGACTTTTAGCTAAAGGAATGATTGATGGGAGAGCGTAT
CCGTGCGCGCCGTAAAAAATTAAAAATCCGCCAGGCAGCGTTAGGCAAGATGGTCGGTGTTTCGAATGTAGCGATCTCT
CAGTGGGAGCGTAGCGAAACTGAACCGAATGGAGAAAATTTATTGGCTTTATCTAAGGCACTGCAGTGTAGTCCGGACT
ACTTGTTGAAAGGTGATCTGAGTCAAACAAACGTCGCGTACCATTCTCGCCATGAACCACGTGGCTCTTATCCTCTGAT
TAGCTGGGTGTCTGCGGGGCAGTGGATGGAGGCGGTCGAACCGTATCACAAGCGTGCAATCGAGAATTGGCATGATACG
ACTGTAGACTGTAGCGAGGATTCGTTCTGGTTGGATGTTCAAGGGGATAGCATGACCGCACCCGCCGGATTAAGCATCC
CCGAAGGCATGATTATTTTGGTCGATCCGGAAGTGGAGCCTCGCAACGGCAAACTTGTCGTGGCTAAGTTGGAAGGCGA
GAATGAGGCGACGTTTAAGAAACTTGTCATGGATGCCGGACGTAAGTTTTTGAAACCCTTAAATCCGCAATATCCGATG
ATCGAGATCAATGGAAACTGCAAAATTATCGGTGTAGTAGTTGATGCCAAGCTGGCTAACTTACCGTAGTAGAGATCAA
GCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACA
GAGAGCTAACTTTATAACTTGACAAATTGGATAGTTTTAGGTATAATAACTTGACAAATTGGATAGTTAGGAGTCAATT
AATGTGCTTTTAATTCTGATGAGACGGTGACGTCGAAACTCCCTCTACAAATAATTTTGTTTAACAGGTTATAAGGCCT
GCAGCGTATGTCAAACACGATAAGCGAGAAGATAGTCTTAATGCGAAAATCAGAGTATTTGAGCAGACAACAACTTGCT
GATTTAACAGGGGTTCCGTATGGCACGCTGAGTTACTATGAAAGTGGTCGTTCAACACCTCCAACAGATGTCATGATGA
ACATCCTGCAGACCCCACAATTCACCAAATACACTTTATGGTTCATGACCAATCAGATCGCTCCTGAGTCCGGGCAAAT
TGCGCCCGCTCTCGCACACTTTGGGCAAAACGAAACAACGTCGCCCCACTCCGGTCAAAAGACTGGTTAATAATCATTC
TTAGCGTGACCGGGAAGTCGGTCACGCTACCTCTTCTGAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGT
TTTTTCTGCTTTTCTAGTGATTGTCAGAAATGGCACTTGCAAAAACTTTCAGTTCAACCATAATACGTACTGAAAGTAC
GAAAAAGGAAGTAGAAGTCAATTAATGTGCTTTTAATTCTGATGAGTCGGTGACGACGAAACTTCCTCTACAAATAATT
TTGTTTAATACTAGAGAAAGAGGGGAAATACTAGATGGCTTCCTCCGAAGACGTTATCAAAGAGTTCATGCGTTTCAAA
GTTCGTATGGAAGGTTCCGTTAACGGTCACGAGTTCGAAATCGAAGGTGAAGGTGAAGGTCGTCCGTACGAAGGTACCC
AGACCGCTAAACTGAAAGTTACCAAAGGTGGTCCGCTGCCGTTCGCTTGGGACATCCTGTCCCCGCAGTTCCAGTACGG
TTCCAAAGCTTACGTTAAACACCCGGCTGACATCCCGGACTACCTGAAACTGTCCTTCCCGGAAGGTTTCAAATGGGAA
CGTGTTATGAACTTCGAAGACGGTGGTGTTGTTACCGTTACCCAGGACTCCTCCCTGCAAGACGGTGAGTTCATCTACA
AAGTTAAACTGCGTGGTACCAACTTCCCGTCCGACGGTCCGGTTATGCAGAAAAAAACCATGGGTTGGGAAGCTTCCAC
CGAACGTATGTACCCGGAAGACGGTGCTCTGAAAGGTGAAATCAAAATGCGTCTGAAACTGAAAGACGGTGGTCACTAC
GACGCTGAAGTTAAAACCACCTACATGGCTAAAAAACCGGTTCAGCTGCCGGGTGCTTACAAAACCGACATCAAACTGG
ACATCACCTCCCACAACGAAGACTACACCATCGTTGAACAGTACGAACGTGCTGAAGGTCGTCACTCCACCGGTGCTTA
AGATGTGGGGTCCGATCTAACTAAAAAGGCCGCTCTGCGGCCTTTTTTCTTTTCACTTCGAGCTCATGTATGTGTCTAC
GCGAGATTCTCGCCCGAGAACTTCTGCAAGGCACTGCTCTTGGCTAGGCGGTAATACGGTTATCCACAGAATCAGGGGA
TAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAATGATTCGCACGGGCCCATGGCT
AATTCCCATGTCAGCCGTTAAGTGTTCCTGTGTCACTCAAAATTGCTTTGAGAGGCTCTAAGGGCTTCTCAGTGCGTTA
CATCCCTGGCTTGTTGTCCACAACCGTTAAACCTTAAAAGCTTTAAAAGCCTTATATATTCTTTTTTTTCTTATAAAAC
TTAAAACCTTAGAGGCTATTTAAGTTGCTGATTTATATTAATTTTATTGTTCAAACATGAGAGCTTAGTACGTGAAACA
TGAGAGCTTAGTACGTTAGCCATGAGAGCTTAGTACGTTAGCCATGAGGGTTTAGTTCGTTAAACATGAGAGCTTAGTA
CGTTAAACATGAGAGCTTAGTACGTGAAACATGAGAGCTTAGTACGTACTATCAACAGGTTGAACTGCTGATCTTCAGA
TCCTCTACGCCGGACGCATCGTGGCCGGATCTTCTTGTAGCTTATCGGCCAGCCTCGCAGAGCAGGATTCCCGTTGAGC
ACCGCCAGGTGCGAATAAGGGACAGTGAAGAAGGAACACCCGCTCGCGGGTGGGCCTACTTCACCTATCCTGCCCGGCT
GACGCCGTTGGATACACCAAGGAAAGTCTACACGAACCCTTTGGCAAAATCCTGTATATCGTGCGAAAAAGGATGGATA
TACCGAAAAAATCGCTATAATGACCCCGAAGCAGGGTTATGCAGCGGAAAGTATACCTTAAGCTTGGATTCTCACCAAT
AAAAAACGCCCGGCGGCAACCGAGCGTTCTGAACAAATCCAGATGGAGTTCTGAGGTCATTACTGGATCTATCAACAGG
AGTCCAAGCGAGCTTTAGAAAAACTCATCGAGCATCAAATGAAACTGCAATTTATTCATATCAGGATTATCAATACCAT
ATTTTTGAAAAAGCCGTTTCTGTAATGAAGGAGAAAACTCACCGAGGCAGTTCCATAGGATGGCAAGATCCTGGTATCG
GTCTGCGATTCCGACTCGTCCAACATCAATACAACCTATTAATTTCCCCTCGTCAAAAATAAGGTTATCAAGTGAGAAA
TCACCATGAGTGACGACTGAATCCGGTGAGAATGGCAAAAGTTTATGCATTTCTTTCCAGACTTGTTCAACAGGCCAGC
CATTACGCTCGTCATCAAAATCACTCGCATCAACCAAACCGTTATTCATTCGTGATTGCGCCTGAGCGAGACGAAATAC
GCGGTCGCTGTTAAAAGGACAATTACAAACAGGAATCGAATGCAACCGGCGCAGGAACACTGCCAGCGCATCAACAATA
TTTTCACCTGAATCAGGATATTCTTCTAATACCTGGAATGCTGTTTTCCCGGGGATCGCAGTGGTGAGTAACCATGCAT
CATCAGGAGTACGGATAAAATGCTTGATGGTCGGAAGAGGCATAAATTCCGTCAGCCAGTTTAGTCTGACCATCTCATC
TGTAACATCATTGGCAACGCTACCTTTGCCATGTTTCAGAAACAACTCTGGCGCATCGGGCTTCCCATACAATCGATAG
ATTGTCGCACCTGATTGCCCGACATTATCGCGAGCCCATTTATACCCATATAAATCAGCATCCATGTTGGAATTTAATC
GCGGCCTAGAGCAAGACGTTTCCCGTTGAATATGGCTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGG
TTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAA