LOCUS g531_big_boy_ci 7953 bp DNA linear 11-Apr-2026 ACCESSION g531_big_boy_cir_synthetic_circular_DNA_comp VERSION g531_big_boy_cir_synthetic_circular_DNA_comp.1 FEATURES Location/Qualifiers misc_feature 2035..2110 /label=BydvJ terminator 3549..3682 /label=DT56 terminator complement(172..256) /label=DT65 terminator 5048..5150 /label=DT86 terminator 6018..6061 /label=ECK120017009 terminator 2968..3057 /label=ECK120029600 promoter 1945..2034 /label=PCymRC promoter complement(1074..1135) /label=PJR1 promoter 3062..3141 /label=PJR3b promoter 3687..3754 /label=PJR4 promoter 4583..4650 /label=PJR4 promoter 5155..5222 /label=PJR7 promoter 1140..1213 /label=Ptet misc_feature 6220..6669 /label=R6K misc_feature 3146..3224 /label=RiboJ60 misc_feature 4651..4725 /label=RiboJ64 misc_feature 1218..1292 /label=RiboJ69 misc_feature 3759..3833 /label=RiboJ71 misc_feature 6066..6128 /label=attP2 terminator 4494..4582 /label=ilvGEDA CDS 3858..4493 /label=jr2 CDS 1315..1857 /label=jr3 CDS 2131..2967 /label=jr4 CDS 3249..3548 /label=jr8 CDS 4748..5047 /label=jr9 CDS complement(7046..7861) /label=kanR misc_feature 6675..6934 /label=oriT CDS 5328..6005 /label=rfp terminator 1858..1940 /label=rpoC CDS complement(257..976) /label=yfp ORIGIN 1 TAGGCATGGT CTAAGAAACC ATTATTATCA TGACATTAAC CTATAAAAAT AGAAGTTCCT 61 ATTCCGAAGT TCCTATTCTC TAGAAAGTAT AGGAACTTCG GATCCGAATT CGAGCTCCGT 121 CGACACTAGT GCGGCCGCAC TCGAGGGCAT CAGAGCAGAT TGTACTGGTA CGCGGAGAAA 181 AGGTCCGAAA ATTCGGACCC GATGGAATTG GATAAAAAAA GGCCCCTGTT GAAATTGCAG 241 GGGCCTGGTA CGAGCATTAC TTGTACAGCT CGTCCATGCC GAGAGTGATC CCGGCGGCGG 301 TCACGAACTC CAGCAGGACC ATGTGATCGC GCTTCTCGTT GGGGTCTTTG CTCAGGGCGG 361 ACTGGTAGCT AAGGTAGTGG TTGTCGGGCA GCAGCACGGG GCCGTCGCCG ATTGGGGTGT 421 TCTGCTGGTA GTGGTCGGCG AGCTGCACGC TGCCGTCCTC GATGTTGTGG CGGATCTTGA 481 AGTTCACCTT GATGCCGTTC TTCTGCTTGT CGGCCATGAT ATAGACGTTG TGGCTGTTGT 541 AGTTGTACTC CAGCTTGTGC CCCAGGATGT TGCCGTCCTC CTTGAAGTCG ATGCCCTTCA 601 GCTCGATGCG GTTCACCAGG GTGTCGCCCT CGAACTTCAC CTCGGCGCGG GTCTTGTAGT 661 TGCCGTCGTC CTTGAAGAAG ATGGTGCGCT CCTGGACGTA GCCTTCGGGC ATGGCGGACT 721 TGAAGAAGTC GTGCAGCTTC ATGTGGTCGG GGTAGCGGGC GAAGCATTGC AGGCCGTAGC 781 CGAAGGTGGT CACGAGGGTG GGCCAGGGCA CGGGCAGCTT GCCTGTGGTG CAGATGAACT 841 TCAGGGTCAG CTTGCCGTAG GTGGCATCGC CCTCGCCCTC GCCGGACACG CTGAACTTGT 901 GGCCGTTTAC GTCGCCGTCC AGCTCGACCA GGATGGGCAC CACCCCGGTG AACAGCTCCT 961 CGCCCTTGCT CACCATCTAG TATTTCTCCT CTTTCTCTAG TATTAAACAA AATTATTTGT 1021 AGAGGCTGTT TCGTCCTCAC GGACTCATCA GACCGGAAAG CACATCCGGT GACAGCTATT 1081 ATCACCGCCA GAGGTAAAAT AGTCAACACG CACGGTGTTA CCCGGCCTGA AAGAAAAGTT 1141 ACTCCACCGT TGGCTTTTTT CCCTATCAGT GATAGAGATT GACATCCCTA TCAGTGATAG 1201 AGATAATGAG CACTCAAAGC TGTCAAGGCG TGTGCTTCGC CTTCTGATGA GCCCGTGAGG 1261 GCGAAACAGC CTCTACAAAT AATTTTGTTT AAGCTGGTAC TGCTCGAGTA TCTTATGAAG 1321 ACTGACACAT CAAATCGCTT AAAGCAGATC ATGGCTGAGC GCAACCTTAA ACAAGTGGAT 1381 ATCCTGAATC TTAGCATCCC TTTTCAAAAA AAATTCGGGA TCAAATTGAG CAAGTCCACC 1441 CTTTCGCAAT ACGTCAACTC GGTACAATCC CCAGACCAGA ACCGTATTTA CCTGTTAGCC 1501 AAGACCTTGG GGGTAAGTGA AGCATGGTTG ATGGGATTCG ACGTTCCAAT GGTGGAATCC 1561 TCGAAGATTG AGAATGATTC AGAGAACATT GAGGAGACGA TCACCGTCAT GAAGAAGCTG 1621 GAAGAGCCGC GTCAGAAAGT GGTCTTGGAT ACAGCAAAAA TTCAGTTAAA GGAGCAGGAT 1681 GAACAGAATA AGGTAAAGCA GATCGAGGAC TATCGCTTAT CCGACGAATA TTTGGAGGAG 1741 CAGATCAGCA AGGCGTCGGC TTACGGAGGG GGGCAACTTA ACGATAATGA TAAGGAATTT 1801 TTCAAACGTC TGCTGAAAAA CACCCTTAAG GAGAAAATTG ACAAAGGCGA TCTTTGAGTA 1861 ATCGTTAATC CGCAAATAAC GTAAAAACCC GCTTCGGCGG GTTTTTTTAT GGGGGGAGTT 1921 TAGGGAAAGA GCATTTGTCA GGAAAACAAA CAGACAATCT GGTCTGTTTG TATTATGGAA 1981 AATTTTTCTG TATAATAGAT TCAACAAACA GACAATCTGG TCTGTTTGTA TTATAGGGTG 2041 TCTCAAGGTG CGTACCTTGA CTGATGAGTC CGAAAGGACG AAACACCCCT CTACAAATAA 2101 TTTTGTTTAA TATTTTTAAT AAATAGGGTT ATGAAGAAGA TTCGCCTTCC CGAAATGATT 2161 GACTACTTCC GTAAGGAAAA CGGCTGGACT ATGAAAGAGT TTGGTGAAAA ATTAGGGAAG 2221 AGTGAGAGCG CTATTAGCAA GTGGATCAAG GGGGTACGCT CCCCTATGGT AGAGGATTTT 2281 GACAAGATGG TGAATTTATT TAACACTGAC CCAGAAACCC TGATGTATGG TGCGTCAGAT 2341 TTATCTACGA CGTTGAGCGA AATCAATAAG ATTTCGAGCC AACTTGAGGA ACCGCGCCAG 2401 AAGGTTGTAC TGAATACGGC AAATAATCAG CTTGACGAAC AAAATCAGGA AAAGAAAAAG 2461 GAATCTAAAG TCATCCCTAT TAACAAAATC CCAGATGACT TACCACCCTA CATTAGTCGC 2521 AAAATTCTGG AAAATTTTGT AATGCCGACT AACACTATGG AATACGAAGC TGATGAAGAT 2581 ATGGTGGACG TACCCATCCT TGGGCGCATC GCGGCGGGCC TTCCGCTTGA CGCGGTAGAA 2641 AACTTTGATG GAACGCGCCC AGTCCCTGCA CATTTCCTTT CGAGTGCCCG TGATTACTAT 2701 TGGCTTATGG TGGACGGCCA TTCTATGGAA CCTAAGATTC CGTACGGAGC ATACGTCTTG 2761 ATCGAAGCCG TCCCCGATGT GAGCGATGGG ACCATTGGTG CCGTCTTATT CCATGATGAT 2821 TGTCAGGCGA CGCTGAAAAA GGTATATCAC GAGATCGACT GTTTGCGCTT AGTCTCAATT 2881 AACAAAGAAT TTAAGGACCA ATTCGCCACG CAAGATAATC CAGCTGCAGT GATTGGTCAA 2941 GCAGTTAAGG TTGAAATCGA CTTGTAATTC AGCCAAAAAA CTTAAGACCG CCGGTCTTGT 3001 CCACTACCTT GCAGTAATGC GGTGGACAGG ATCGGCGGTT TTCTTTTCTC TTCTCAAAGG 3061 AGCCACAAGC TCGAAAAGTT CATGAAACGT GAACTTGACA GCTAGCTCAG TCCTAGGTAT 3121 AAAGTTCATG AAACGTGAAC TCCACCTGAA GTCGTCAAGT GCTGTGCTTG CACTTCTGAT 3181 GAGGCAGTGA TGCCGAAACG ACCTCTACAA ATAATTTTGT TTAATTATCT TAGGTACAGG 3241 GCACATAAAT GTCAATAGAC GTTTCGGAGA AGTTGAAGCT AATCCGTGAA TCTGAAAGGT 3301 TAAACCGTAA AGAATTCAGT GAATTAACTG GTGTAGCCTA CAGCTCACTT TCGAGCTATG 3361 AGAGCCGGTC AAAAAACGCT GGAGTTGAAG CCATAATGAA GGTCTTACAA CATCCTAGAT 3421 TTACTAAATA TACTTTGTGG TTCATGACTG ATCAGGTAGC TCCAGAAGCC GGGCAAATTG 3481 CGCCCGCTCT CGCACACTTT GGGCAAAACG AAACAACGTC GCCCCACTCC GGTCAAAAGA 3541 CTGGTTAATA CCACCGTCAA AAAAAACGGC GCTTTTTAGC GCCGTTTTTA TTTTTCAACC 3601 TTCCAGGCAT CAAATAAAAC GAAAGGCTCA GTCGAAAGAC TGGGCCTTTC GTTTTATCTG 3661 TTGTTTGTCG GTGAACGCTC TCAACCGAGA GCTAACTTTA TAACTTGACA AATTGGATAG 3721 TTTTAGGTAT AATAACTTGA CAAATTGGAT AGTTGCCCAG TTGTCAGCCA CTGTGCTTGT 3781 GGCTCTGATG AGACGGTGAC GTCGAAACAA CCTCTACAAA TAATTTTGTT TAAGACTTTT 3841 AGCTAAAGGA ATGATTGATG GGAGAGCGTA TCCGTGCGCG CCGTAAAAAA TTAAAAATCC 3901 GCCAGGCAGC GTTAGGCAAG ATGGTCGGTG TTTCGAATGT AGCGATCTCT CAGTGGGAGC 3961 GTAGCGAAAC TGAACCGAAT GGAGAAAATT TATTGGCTTT ATCTAAGGCA CTGCAGTGTA 4021 GTCCGGACTA CTTGTTGAAA GGTGATCTGA GTCAAACAAA CGTCGCGTAC CATTCTCGCC 4081 ATGAACCACG TGGCTCTTAT CCTCTGATTA GCTGGGTGTC TGCGGGGCAG TGGATGGAGG 4141 CGGTCGAACC GTATCACAAG CGTGCAATCG AGAATTGGCA TGATACGACT GTAGACTGTA 4201 GCGAGGATTC GTTCTGGTTG GATGTTCAAG GGGATAGCAT GACCGCACCC GCCGGATTAA 4261 GCATCCCCGA AGGCATGATT ATTTTGGTCG ATCCGGAAGT GGAGCCTCGC AACGGCAAAC 4321 TTGTCGTGGC TAAGTTGGAA GGCGAGAATG AGGCGACGTT TAAGAAACTT GTCATGGATG 4381 CCGGACGTAA GTTTTTGAAA CCCTTAAATC CGCAATATCC GATGATCGAG ATCAATGGAA 4441 ACTGCAAAAT TATCGGTGTA GTAGTTGATG CCAAGCTGGC TAACTTACCG TAGTAGAGAT 4501 CAAGCCTTAA CGAACTAAGA CCCCCGCACC GAAAGGTCCG GGGGTTTTTT TTGACCTTAA 4561 AAACATAACC GAGGAGCAGA CAGAGAGCTA ACTTTATAAC TTGACAAATT GGATAGTTTT 4621 AGGTATAATA ACTTGACAAA TTGGATAGTT AGGAGTCAAT TAATGTGCTT TTAATTCTGA 4681 TGAGACGGTG ACGTCGAAAC TCCCTCTACA AATAATTTTG TTTAACAGGT TATAAGGCCT 4741 GCAGCGTATG TCAAACACGA TAAGCGAGAA GATAGTCTTA ATGCGAAAAT CAGAGTATTT 4801 GAGCAGACAA CAACTTGCTG ATTTAACAGG GGTTCCGTAT GGCACGCTGA GTTACTATGA 4861 AAGTGGTCGT TCAACACCTC CAACAGATGT CATGATGAAC ATCCTGCAGA CCCCACAATT 4921 CACCAAATAC ACTTTATGGT TCATGACCAA TCAGATCGCT CCTGAGTCCG GGCAAATTGC 4981 GCCCGCTCTC GCACACTTTG GGCAAAACGA AACAACGTCG CCCCACTCCG GTCAAAAGAC 5041 TGGTTAATAA TCATTCTTAG CGTGACCGGG AAGTCGGTCA CGCTACCTCT TCTGAAGAAA 5101 CAGCAAACAA TCCAAAACGC CGCGTTCAGC GGCGTTTTTT CTGCTTTTCT AGTGATTGTC 5161 AGAAATGGCA CTTGCAAAAA CTTTCAGTTC AACCATAATA CGTACTGAAA GTACGAAAAA 5221 GGAAGTAGAA GTCAATTAAT GTGCTTTTAA TTCTGATGAG TCGGTGACGA CGAAACTTCC 5281 TCTACAAATA ATTTTGTTTA ATACTAGAGA AAGAGGGGAA ATACTAGATG GCTTCCTCCG 5341 AAGACGTTAT CAAAGAGTTC ATGCGTTTCA AAGTTCGTAT GGAAGGTTCC GTTAACGGTC 5401 ACGAGTTCGA AATCGAAGGT GAAGGTGAAG GTCGTCCGTA CGAAGGTACC CAGACCGCTA 5461 AACTGAAAGT TACCAAAGGT GGTCCGCTGC CGTTCGCTTG GGACATCCTG TCCCCGCAGT 5521 TCCAGTACGG TTCCAAAGCT TACGTTAAAC ACCCGGCTGA CATCCCGGAC TACCTGAAAC 5581 TGTCCTTCCC GGAAGGTTTC AAATGGGAAC GTGTTATGAA CTTCGAAGAC GGTGGTGTTG 5641 TTACCGTTAC CCAGGACTCC TCCCTGCAAG ACGGTGAGTT CATCTACAAA GTTAAACTGC 5701 GTGGTACCAA CTTCCCGTCC GACGGTCCGG TTATGCAGAA AAAAACCATG GGTTGGGAAG 5761 CTTCCACCGA ACGTATGTAC CCGGAAGACG GTGCTCTGAA AGGTGAAATC AAAATGCGTC 5821 TGAAACTGAA AGACGGTGGT CACTACGACG CTGAAGTTAA AACCACCTAC ATGGCTAAAA 5881 AACCGGTTCA GCTGCCGGGT GCTTACAAAA CCGACATCAA ACTGGACATC ACCTCCCACA 5941 ACGAAGACTA CACCATCGTT GAACAGTACG AACGTGCTGA AGGTCGTCAC TCCACCGGTG 6001 CTTAAGATGT GGGGTCCGAT CTAACTAAAA AGGCCGCTCT GCGGCCTTTT TTCTTTTCAC 6061 TTCGAGCTCA TGTATGTGTC TACGCGAGAT TCTCGCCCGA GAACTTCTGC AAGGCACTGC 6121 TCTTGGCTAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 6181 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAT GATTCGCACG GGCCCATGGC 6241 TAATTCCCAT GTCAGCCGTT AAGTGTTCCT GTGTCACTCA AAATTGCTTT GAGAGGCTCT 6301 AAGGGCTTCT CAGTGCGTTA CATCCCTGGC TTGTTGTCCA CAACCGTTAA ACCTTAAAAG 6361 CTTTAAAAGC CTTATATATT CTTTTTTTTC TTATAAAACT TAAAACCTTA GAGGCTATTT 6421 AAGTTGCTGA TTTATATTAA TTTTATTGTT CAAACATGAG AGCTTAGTAC GTGAAACATG 6481 AGAGCTTAGT ACGTTAGCCA TGAGAGCTTA GTACGTTAGC CATGAGGGTT TAGTTCGTTA 6541 AACATGAGAG CTTAGTACGT TAAACATGAG AGCTTAGTAC GTGAAACATG AGAGCTTAGT 6601 ACGTACTATC AACAGGTTGA ACTGCTGATC TTCAGATCCT CTACGCCGGA CGCATCGTGG 6661 CCGGATCTTC TTGTAGCTTA TCGGCCAGCC TCGCAGAGCA GGATTCCCGT TGAGCACCGC 6721 CAGGTGCGAA TAAGGGACAG TGAAGAAGGA ACACCCGCTC GCGGGTGGGC CTACTTCACC 6781 TATCCTGCCC GGCTGACGCC GTTGGATACA CCAAGGAAAG TCTACACGAA CCCTTTGGCA 6841 AAATCCTGTA TATCGTGCGA AAAAGGATGG ATATACCGAA AAAATCGCTA TAATGACCCC 6901 GAAGCAGGGT TATGCAGCGG AAAGTATACC TTAAGCTTGG ATTCTCACCA ATAAAAAACG 6961 CCCGGCGGCA ACCGAGCGTT CTGAACAAAT CCAGATGGAG TTCTGAGGTC ATTACTGGAT 7021 CTATCAACAG GAGTCCAAGC GAGCTTTAGA AAAACTCATC GAGCATCAAA TGAAACTGCA 7081 ATTTATTCAT ATCAGGATTA TCAATACCAT ATTTTTGAAA AAGCCGTTTC TGTAATGAAG 7141 GAGAAAACTC ACCGAGGCAG TTCCATAGGA TGGCAAGATC CTGGTATCGG TCTGCGATTC 7201 CGACTCGTCC AACATCAATA CAACCTATTA ATTTCCCCTC GTCAAAAATA AGGTTATCAA 7261 GTGAGAAATC ACCATGAGTG ACGACTGAAT CCGGTGAGAA TGGCAAAAGT TTATGCATTT 7321 CTTTCCAGAC TTGTTCAACA GGCCAGCCAT TACGCTCGTC ATCAAAATCA CTCGCATCAA 7381 CCAAACCGTT ATTCATTCGT GATTGCGCCT GAGCGAGACG AAATACGCGG TCGCTGTTAA 7441 AAGGACAATT ACAAACAGGA ATCGAATGCA ACCGGCGCAG GAACACTGCC AGCGCATCAA 7501 CAATATTTTC ACCTGAATCA GGATATTCTT CTAATACCTG GAATGCTGTT TTCCCGGGGA 7561 TCGCAGTGGT GAGTAACCAT GCATCATCAG GAGTACGGAT AAAATGCTTG ATGGTCGGAA 7621 GAGGCATAAA TTCCGTCAGC CAGTTTAGTC TGACCATCTC ATCTGTAACA TCATTGGCAA 7681 CGCTACCTTT GCCATGTTTC AGAAACAACT CTGGCGCATC GGGCTTCCCA TACAATCGAT 7741 AGATTGTCGC ACCTGATTGC CCGACATTAT CGCGAGCCCA TTTATACCCA TATAAATCAG 7801 CATCCATGTT GGAATTTAAT CGCGGCCTAG AGCAAGACGT TTCCCGTTGA ATATGGCTCA 7861 TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT 7921 ACATATTTGA ATGTATTTAG AAAAATAAAC AAA //